neu3.5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	neu3.5
Entrez Gene ID	100000684
Full Name	sialidase 3 (membrane sialidase), tandem duplicate 5
Synonyms	si:dkey-11j5.6
General protein information	Preferred Names sialidase 3 (membrane sialidase), tandem duplicate 5 Names uncharacterized protein LOC100000684 sialidase 3, tandem duplicate 5 sialidase 3.5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005161128.3	XP_005161185.1	uncharacterized protein LOC100000684 isoform X1
NM_001109733.1	NP_001103203.1	uncharacterized protein LOC100000684

Pathways from BioSystems

Homologies

Homo sapiens (human)	NEU3	NP_006647.3
Canis lupus familiaris (dog)	NEU3	XP_005633558.1
Gallus gallus (chicken)	NEU3	XP_428099.4
Danio rerio (zebrafish)	neu3.4	NP_001103193.1
Bos taurus (cattle)	NEU3	NP_776547.2
Danio rerio (zebrafish)	neu3.1	NP_001073133.1
Rattus norvegicus (Norway rat)	Neu3	NP_446462.1
Macaca mulatta (Rhesus monkey)	LOC694310	XP_002808128.1
Danio rerio (zebrafish)	neu3.2	NP_001003644.2
Danio rerio (zebrafish)	neu3.5	NP_001103203.1
Pan troglodytes (chimpanzee)	NEU3	XP_003952031.1
Mus musculus (house mouse)	Neu3	NP_057929.1
Danio rerio (zebrafish)	neu3.3	NP_001071006.1
Xenopus tropicalis (tropical clawed frog)	LOC100496715	XP_002942460.1

Gene Ontology
Bibliography

Related articles in PubMed

Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns.
Yamakawa N, Vanbeselaere J, Chang LY, Yu SY, Ducrocq L, Harduin-Lepers A, Kurata J, Aoki-Kinoshita KF, Sato C, Khoo KH, Kitajima K, Guerardel Y
Nature communications9(1)4647(2018 11)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Molecular cloning and biochemical characterization of two novel Neu3 sialidases, neu3a and neu3b, from medaka (Oryzias latipes).
Shiozaki K, Takeshita K, Ikeda M, Ikeda A, Harasaki Y, Komatsu M, Yamada S, Yamaguchi K, Miyagi T
Biochimie95(2)280-9(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following neu3.5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the neu3.5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03667	NM_001109733.1 Latest version!	Danio rerio sialidase 3 (membrane sialidase), tandem duplicate 5 (neu3.5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa25717	XM_005161128.3 Latest version!	Danio rerio sialidase 3 (membrane sialidase), tandem duplicate 5 (neu3.5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

neu3.5 ( NM_001109733.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001103203.1 Danio rerio sialidase 3 (membrane sialidase), tandem duplicate 5 (neu3.5), mRNA.

Price & Availability↑

CloneID	ODa03667
Clone ID Related Accession (Same CDS sequence)	NM_001109733.1
Accession Version	NM_001109733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550332800000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100000684
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from EF107694.1. On Oct 5, 2007 this sequence version replaced XM_001340799.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATGGGTT GCTGTTGCCT GTCACGCCAT CCATCAAGCT ATTCAGCTCC TCCCGAAATA 
TCAATATTAT TCAAACAGGA ACTGCCTCAA TGTAAAAAAC AAGTGACCTA CAGGATTCCA 
GCTCTCATAT ATATCAGTGA TAAACAAACC TTCCTTGCCT TTGCTGAAAG GCGGAAAACC 
CCAAGTGACA CAGATGCAGA ATTGCTTGTG ATGAGAAAAG GATTGTGGAA GAATGACAAT 
AATCCAGTTA AGTGGGTCAG TGGTCAACAG GTGTTTACGT CAGCTTGTCT CCCAAACTAC 
CGCAGCATGA ATCCATGTCC GGTCTATGAG AGAAAGTCCA AAACCCTCTT TCTGTTTTTC 
ATTTGTGTCC CTGTTGGGGT CTCTGAACAG CAACAAAAAA ACGAGAAAAA GAATCAGGCA 
CGCCTTTGTT ACATAACCAG TAAGGACACT GGTGAAACCT GGAGTGCTGT TACTGATTTG 
ACAGCAGATG TGATCGGTGA GCAGGTGAAG AACTGGGCCA CCTTTGCTGT TGGACCAGGA 
CATGGAATTC AGATGAAGAG TGGGAGACTG ATTATCCCAG CGTACACGTA TTATTTCACC 
TCAGAAAGTC CACCCACATC GCATGCACTT GCAATCTACA GTGATGATAA AGGGAGCACT 
TGGCATATCG GAAAGTGTAT GAATGGTCTA CCTTCAAATG AATGTCAGAT GGCTGAGATC 
ATCGATGATG AAGGCAACAG TAAACTTTAC TGCAATGCTC GAAGCACAAT TCACTACAGG 
GTCCAGGCTC TAAGTGAAAG TAAAGGAACA GATTTTGACA CGTCTTCATC TGTAAATAAA 
CTGATAGAGA CTGGAAATGG TTGCCAAGGA AGCGTGTTGA GTTTTACCCC TGATCAAGCA 
TCTGAAAAAA CATGGCTGCT ATACTGCCAC CCAACCAATG CAACCAATCG ATTAACCCTT 
GGCATATACT TAAATAAATG CGCGAGTGAC TTGTCTGGAT GGGGTAATCC TGTGATTCTG 
CATGAGGGTC CAAGTGGATA CAGTGATCTG ACTCAGTGTG GGGATGGAAA ACACTTTGCC 
TGTCTCATGG AATGTGGAGA GAAAAGTGAG CTTGAAGGAA TAGGCTTTAT CCTGTTTGAA 
CAGAAAGATG ACAAGGCCGA AAAATTACAA ATTACCAAAT TTACTAAACA GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103203.1
CDS	1..1197
Translation	MMGCCCLSRHPSSYSAPPEISILFKQELPQCKKQVTYRIPALIYISDKQTFLAFAERRKTPSDTDAELLVMRKGLWKNDNNPVKWVSGQQVFTSACLPNYRSMNPCPVYERKSKTLFLFFICVPVGVSEQQQKNEKKNQARLCYITSKDTGETWSAVTDLTADVIGEQVKNWATFAVGPGHGIQMKSGRLIIPAYTYYFTSESPPTSHALAIYSDDKGSTWHIGKCMNGLPSNECQMAEIIDDEGNSKLYCNARSTIHYRVQALSESKGTDFDTSSSVNKLIETGNGCQGSVLSFTPDQASEKTWLLYCHPTNATNRLTLGIYLNKCASDLSGWGNPVILHEGPSGYSDLTQCGDGKHFACLMECGEKSELEGIGFILFEQKDDKAEKLQITKFTKQN

Target ORF information:

RefSeq Version	NM_001109733.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sialidase 3 (membrane sialidase), tandem duplicate 5 (neu3.5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109733.1

ORF Insert Sequence:

ATGATGGGTT GCTGTTGCCT GTCACGCCAT CCATCAAGCT ATTCAGCTCC TCCCGAAATA 
TCAATATTAT TCAAACAGGA ACTGCCTCAA TGTAAAAAAC AAGTGACCTA CAGGATTCCA 
GCTCTCATAT ATATCAGTGA TAAACAAACC TTCCTTGCCT TTGCTGAAAG GCGGAAAACC 
CCAAGTGACA CAGATGCAGA ATTGCTTGTG ATGAGAAAAG GATTGTGGAA GAATGACAAT 
AATCCAGTTA AGTGGGTCAG TGGTCAACAG GTGTTTACGT CAGCTTGTCT CCCAAACTAC 
CGCAGCATGA ATCCATGTCC GGTCTATGAG AGAAAGTCCA AAACCCTCTT TCTGTTTTTC 
ATTTGTGTCC CTGTTGGGGT CTCTGAACAG CAACAAAAAA ACGAGAAAAA GAATCAGGCA 
CGCCTTTGTT ACATAACCAG TAAGGACACT GGTGAAACCT GGAGTGCTGT TACTGATTTG 
ACAGCAGATG TGATCGGTGA GCAGGTGAAG AACTGGGCCA CCTTTGCTGT TGGACCAGGA 
CATGGAATTC AGATGAAGAG TGGGAGACTG ATTATCCCAG CGTACACGTA TTATTTCACC 
TCAGAAAGTC CACCCACATC GCATGCACTT GCAATCTACA GTGATGATAA AGGGAGCACT 
TGGCATATCG GAAAGTGTAT GAATGGTCTA CCTTCAAATG AATGTCAGAT GGCTGAGATC 
ATCGATGATG AAGGCAACAG TAAACTTTAC TGCAATGCTC GAAGCACAAT TCACTACAGG 
GTCCAGGCTC TAAGTGAAAG TAAAGGAACA GATTTTGACA CGTCTTCATC TGTAAATAAA 
CTGATAGAGA CTGGAAATGG TTGCCAAGGA AGCGTGTTGA GTTTTACCCC TGATCAAGCA 
TCTGAAAAAA CATGGCTGCT ATACTGCCAC CCAACCAATG CAACCAATCG ATTAACCCTT 
GGCATATACT TAAATAAATG CGCGAGTGAC TTGTCTGGAT GGGGTAATCC TGTGATTCTG 
CATGAGGGTC CAAGTGGATA CAGTGATCTG ACTCAGTGTG GGGATGGAAA ACACTTTGCC 
TGTCTCATGG AATGTGGAGA GAAAAGTGAG CTTGAAGGAA TAGGCTTTAT CCTGTTTGAA 
CAGAAAGATG ACAAGGCCGA AAAATTACAA ATTACCAAAT TTACTAAACA GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neu3.5 ( XM_005161128.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161185.1 Danio rerio sialidase 3 (membrane sialidase), tandem duplicate 5 (neu3.5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25717
Clone ID Related Accession (Same CDS sequence)	XM_005161128.3
Accession Version	XM_005161128.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100000684 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161128.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTT GCTGTTGCCT GTCACGCCAT TCATCAAGCT ATTCAGCTCC TCCCGAAATA 
TCAATATTAT TCAAACAGGA ACTGCCTCAA TGTAAAAAAC AAGTGACCTA CAGGATTCCA 
GCTCTCATAT ATATCAGTGA TAAACAAACC TTCCTTGCCT TTGCTGAAAG GCGGAAAACC 
CCAAGTGACA CAGATGCAGA ATTGCTTGTG ATGAAAAAAG GATTGTGGAA GAATGACAAT 
AATCCAGTTA AGTGGGTCAG TGGTCAACAG GTGTTTACGT CAGCTTGTCT CCCAAACCAC 
CGCAGCATGA ATCCATGTCC GGTCTATGAG AGAAAGTCCA AAACCCTCTT TCTGTTTTTC 
ATTTGTGTCC CTGTTGGGGT CTCTGAACAG CAACAAAAAA ATGAGAAAAA GAATCAGGCA 
CGCCTTTGTT ACATAACCAG TAAGGACACT GGTGAAACCT GGAGTGCTGT TACTGATTTA 
ACAGCAGATG TGATCGGTGA GCAGGAGAAG AACTGGGCCA CCTTTGCTGT TGGACCAGGA 
CATGGAATTC AGATGAAGAG TGGGAGACTG ATTATCCCAG CGTACACATA TTTTTTCACC 
TCAGAAAATC CACCCACATC GCATGCATTT GCAATCTACA GTGATGATAA AGGGAGCACT 
TGGCATATCG GAAAGTGTAT GAATGGTCTA CCTTCAAATG AATGTCAGAT GGCTGAGATC 
ATCGATGATG AAGGCAACAG TAAACTTTAC TGCAATGCTC GAAGCACAAT TCACTACAGG 
GTCCAGGCTC TAAGTGAAAG TAAAGGAACA GATTTTGACA CGTCTTCATC TGTAAATAAA 
CTGATAGAGA CTGGAAATGG TTGCCAAGGA AGCGTGTTGA GTTTTACCCC TGATCAAGCA 
TCTGAAAAAA CATGGCTGCT ATACTGCCAC CCAACCAATG CAACCAATCG ATTAACCCTT 
GGCGTATACT TAAATAAATG CGCGAGTGAC TTGTCTGGAT GGGGTAATCC TGTGATTCTG 
CATGAGGGTC CAAGTGGATA CAGTGATCTG ACTCAATGTG GGGATGGAAA ACACTTTGCC 
TGTCTCATGG AATGTGGAGA GAAAAGTGAG CTTGAAGGAA TAGGCTTTAT CCTGTTTGAA 
CAGAAAGATG ACAAGGCCGA AAAATTACAA ATTACCAAAT TTACTAAACA GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161185.1
CDS	89..1285
Translation	MMGCCCLSRHSSSYSAPPEISILFKQELPQCKKQVTYRIPALIYISDKQTFLAFAERRKTPSDTDAELLVMKKGLWKNDNNPVKWVSGQQVFTSACLPNHRSMNPCPVYERKSKTLFLFFICVPVGVSEQQQKNEKKNQARLCYITSKDTGETWSAVTDLTADVIGEQEKNWATFAVGPGHGIQMKSGRLIIPAYTYFFTSENPPTSHAFAIYSDDKGSTWHIGKCMNGLPSNECQMAEIIDDEGNSKLYCNARSTIHYRVQALSESKGTDFDTSSSVNKLIETGNGCQGSVLSFTPDQASEKTWLLYCHPTNATNRLTLGVYLNKCASDLSGWGNPVILHEGPSGYSDLTQCGDGKHFACLMECGEKSELEGIGFILFEQKDDKAEKLQITKFTKQN

Target ORF information:

RefSeq Version	XM_005161128.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sialidase 3 (membrane sialidase), tandem duplicate 5 (neu3.5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161128.3

ORF Insert Sequence:

ATGATGGGTT GCTGTTGCCT GTCACGCCAT TCATCAAGCT ATTCAGCTCC TCCCGAAATA 
TCAATATTAT TCAAACAGGA ACTGCCTCAA TGTAAAAAAC AAGTGACCTA CAGGATTCCA 
GCTCTCATAT ATATCAGTGA TAAACAAACC TTCCTTGCCT TTGCTGAAAG GCGGAAAACC 
CCAAGTGACA CAGATGCAGA ATTGCTTGTG ATGAAAAAAG GATTGTGGAA GAATGACAAT 
AATCCAGTTA AGTGGGTCAG TGGTCAACAG GTGTTTACGT CAGCTTGTCT CCCAAACCAC 
CGCAGCATGA ATCCATGTCC GGTCTATGAG AGAAAGTCCA AAACCCTCTT TCTGTTTTTC 
ATTTGTGTCC CTGTTGGGGT CTCTGAACAG CAACAAAAAA ATGAGAAAAA GAATCAGGCA 
CGCCTTTGTT ACATAACCAG TAAGGACACT GGTGAAACCT GGAGTGCTGT TACTGATTTA 
ACAGCAGATG TGATCGGTGA GCAGGAGAAG AACTGGGCCA CCTTTGCTGT TGGACCAGGA 
CATGGAATTC AGATGAAGAG TGGGAGACTG ATTATCCCAG CGTACACATA TTTTTTCACC 
TCAGAAAATC CACCCACATC GCATGCATTT GCAATCTACA GTGATGATAA AGGGAGCACT 
TGGCATATCG GAAAGTGTAT GAATGGTCTA CCTTCAAATG AATGTCAGAT GGCTGAGATC 
ATCGATGATG AAGGCAACAG TAAACTTTAC TGCAATGCTC GAAGCACAAT TCACTACAGG 
GTCCAGGCTC TAAGTGAAAG TAAAGGAACA GATTTTGACA CGTCTTCATC TGTAAATAAA 
CTGATAGAGA CTGGAAATGG TTGCCAAGGA AGCGTGTTGA GTTTTACCCC TGATCAAGCA 
TCTGAAAAAA CATGGCTGCT ATACTGCCAC CCAACCAATG CAACCAATCG ATTAACCCTT 
GGCGTATACT TAAATAAATG CGCGAGTGAC TTGTCTGGAT GGGGTAATCC TGTGATTCTG 
CATGAGGGTC CAAGTGGATA CAGTGATCTG ACTCAATGTG GGGATGGAAA ACACTTTGCC 
TGTCTCATGG AATGTGGAGA GAAAAGTGAG CTTGAAGGAA TAGGCTTTAT CCTGTTTGAA 
CAGAAAGATG ACAAGGCCGA AAAATTACAA ATTACCAAAT TTACTAAACA GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns.
Nature communications9(1)4647(2018 11)
Yamakawa N,Vanbeselaere J,Chang LY,Yu SY,Ducrocq L,Harduin-Lepers A,Kurata J,Aoki-Kinoshita KF,Sato C,Khoo KH,Kitajima K,Guerardel Y

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Molecular cloning and biochemical characterization of two novel Neu3 sialidases, neu3a and neu3b, from medaka (Oryzias latipes).
Biochimie95(2)280-9(2013 Feb)
Shiozaki K,Takeshita K,Ikeda M,Ikeda A,Harasaki Y,Komatsu M,Yamada S,Yamaguchi K,Miyagi T

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neu3.5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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