ORF » Species Summary » Cryptococcus neoformans var. neoformans JEC21 » CNA05130 cDNA ORF clone

CNA05130 cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

The following CNA05130 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNA05130 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OCn00157 XM_566813.2
Latest version!		 Cryptococcus neoformans var. neoformans JEC21 enolase 1, putative (CNA05130), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
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OCn00157 XM_566813.1 Cryptococcus neoformans var. neoformans JEC21 hypothetical protein (CNA05130), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CNA05130 ( XM_566813.1 ) cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21) -> XP_566813.1 Cryptococcus neoformans var. neoformans JEC21 hypothetical protein (CNA05130), partial mRNA.
    Price & Availability↑
    CloneID OCn00157
    Clone ID Related Accession (Same CDS sequence) XM_566813.1 , XM_566813.2
    Accession Version XM_566813.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1345046400000
    Organism Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
    Product hypothetical protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_006670). The reference sequence was derived from mrna.CNA05130.1. This record represents the complete annotated sequence of the C. neoformans JEC21 genome. A small number of gaps exist which are represented by NNNNs within the final sequence. The genome has been annotated by TIGR and the annotation data is available for viewing and download from the TIGR database (http://www.tigr.org/tdb/e2k1/cna1/). COMPLETENESS: incomplete on the 5' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCTTCTG AGAAAGGACA GTCAAACACA AACGAGAAAC AACTTATTAA CCGCGCCGAC 
    ACTGGCAAGA CTGCAGTGTC AGACTCTCCT CTCCCTTTCA AACCCCATAC CGCTCTCTCT 
    GGCAAGATCC TCGAGGCTTT AGGGAGTGAT GCAGCTTCTG GTCTATCAGA TGAGGAAGTA 
    TCAAGGAGAC TCCAACAGTA TGGTCCCAAT AGGCTGAAGC CCCCAAAGAG ACCCAGTATT 
    CTCAAGATCA TCGCTAGACA AGTGGGCAAT GCTATGACTC TTATCCTCAT CGCTGCCATG 
    GCAACTTCAT TGGGTACCAT GGACTGGATC AGCGGTGGCG TTATTGCGGC TCTGGTTATC 
    CTCAATGTAT CAGTGGGAGC CTACACAGAA TGGCAAGCCG AAAAGACCGT GGCCAGCCTC 
    GAGTCTGTGG GAGCTCCGCA AGCTACTGTA GTCCGAACTC GCAAAGGTTC TCGAGACCCT 
    ACCATCAGCA TTATCCCCGT CGAGGAAGTC GTACCCGGCG ACATCATTCA ACTCAAAAAT 
    GGTGATATCG TTCCTGCGGA CGGGAGAATC CTTGACGGGC ATCTGAGTAA CTTGGAAGCT 
    GATGAGGCTT TCCTCACTGG TGAAAGTCTG CCGGTTGCAA AGCAGACTGA GCCTATCGAT 
    GAAGAGGATT GTCCTGTTGG CGACCGTATT TGTATGGTCT TTTCTGGTTC CCAGATCACC 
    AAAGGTCGAG CTCGTGTTGT CATTACCAGC ACTGGTATGG GGACAGAGAT TGGAAAAATT 
    GCTCAAGCTC TTGAATCTAA AGCTCAAAAC AAGAATCGTG GATTTGCAGC TTTCTGGTGG 
    AAACTTAAAG TTATTTTGGG CGTCGAGGAG ACCACTCCTT TGCAAATGAA ACTTAACAAG 
    CTCGCATACT TCCTTTTGGC GTGTGCCCTC GTCATAGCCG TCATTGTTGT CGCCTCCACC 
    GGTTTTAATG ATGTTCCCCT CTCTATTGCC ACCTACGCTG TCGCTGCCGC CGTCTCCATC 
    CTTCCGGCCT CTTTGATTGC GGTTGTCAGT TTGACTTTGG CGCGTGCGTC AACTGATTTA 
    GCATCTCGAC ATGCTTTGGT CAGACGAATG GATGCTATTG AGGCTTTAGC TGGTGTTGAG 
    AATGTGTGCT CGGACAAGAC CGGTACCCTT ACTGTTGGCC GCATGGTAGT TCGCAAAGTC 
    TGGGTTCCTG CTCTCGACTG GCGCCCCAAT GATTTTGCTC CCCTCGACAC TAGTGGTGGT 
    CAAGCATACA GTTTTGAGAC CGGATCTGAC CCTTTCTATC CTCGTGGTGA AGTTCGGGCC 
    GATCCCCAAA AGATCTCTGG GACTGCAGAG ACCCTCGATC TCAAGCAACC CCGTGATCAA 
    TCTGACTCTT CCTCTTCCGA CACTGACCCC GAGGAGCGAG ATGTAGAGTA TCAAGAACGA 
    GTCATCCATG TGGAAGAAAT GGAGAACAAC CTTCGAGATC TTGCTCTCTG TATTTCGCTC 
    TGTAATCAAG CAACTCTCAG TCGTCCCGTA GACCAAGACG GCCAATGGGA AGCAAATGGT 
    GATCCTACCG AAACGGCCCT TCAAGTTGCT GCCCACAAGC TTGGTCATGG CAAGCCTTTT 
    CTTACTCATG TGGCCAAGCC AAGCCATCGT GCGGATTCTA TCCGATCTGG TCAGAGTTCT 
    CGTCCCCTTG TCGCTGGTAT TCGTGGGCAC TTTGTTCAGA TCATTGAGCA TCCTTTCGAT 
    TCCACCGTCA AACGGATGTC TATCGCCTAT AAATTTGTAG GCGAGGATCC TCAGGATTCC 
    CACGTCCTCT GTCTCCTTAA GGGTGCCATC GAGCGTGTCT TTGAGCGATG CACCAAGATC 
    CAAGGACAGC CAATCACCGA AGAGCATAAG AAGGATATCA TGATCAAAGT CGATGCTCTC 
    GCCGCTCAAG GTCTTCGGGT CCTCGCTCTT TGTGGAAAGC GACTTCCTGT CAGCATGGTC 
    GACGAAGTCA AATCTACCCC TCGAGACGCA TTCGAAGCCG ATTTTCACTT CCTCGGTCTT 
    GCTGGTATCT TCGACCCACC CAGAAAGGAA TCTGCTGGCG CTGTTGCTGA TTGTTTCAGG 
    GCTGGTATCA CCCCTCGGAT GTTGACAGGC GATCACCCTG CTACCGCGAC AGCTATCGCT 
    CTCAACATTG GTATTCTTGA TAAGACTTAC TCAAAGACTT CCGTCATGAC AGGTCAGCAA 
    TTCGACTCTT TGAGCGAAGA CGAAATTGAT CAACTGCCCG AGTTGCCTCT TGTCGTTGCT 
    CGCTGCGCCC CCGAGACCAA GGTTCGAATG GTCGATGCCA TTCATCGACG AGGACAAAGC 
    ACAGTCATGA CTGGTGATGG CGTCAACGAC TCCCCCGCCC TCAAGCGTGC CGATGTGGGC 
    GTTGGCATGG GTACTGGTTC CGATGTTGCC AAGCAGTCAG CGCGTATCGT CCTCAGTGAT 
    GACAACTTCA GCACCATCAT TCGAGCTATT AGGAAGGGTC GTTCTGTCTT CAAGAATTTG 
    TCTAAATTCT TGCTCTACTT GCTTTCGGGT AACTTGGCTG AAATCATCGT TCTGATGATT 
    GGTCTCGCTT TCAAGGATGA GAATGGTCAG GCTGTGTTCC CCCTGTCACC TGTTGCCGCT 
    CTTTGGATCA ACACTCTCGC TGCCGGACCT CCAGCCCTTG CCCTAGGTCT TGAACCTACA 
    GCTATCGACG CCATGGAGCA GGGGCCCGAA GTATACCATC AAATCTTTAC CCTCGAATTT 
    TACATCGACC TGATCTTCTA CGGCTTCCTG ATGGGTTCCA TCAGTTTGGT CAACTTCGTC 
    ATTGTACTCT GGGGATACTA TCCTGGAGAT TTAGGTCGTT TTTGTAACGA AGATGATCCC 
    AGCATCTGTG ATCCCGTCTA TCAGGCTCGA GCTGCCTGTT TTGCCACCCT CGTTATTGTC 
    CTCATGATCC ATGCTTTGGA GTGTAAGCAC TTGAACAAAG GGTTGGCCCA AATCAATTTG 
    CGTGACAACA AGGTGTTGCT GTGGTGTGTC GTTGTCCTCA GTCTTTCCGC TTTCCCTGTC 
    GTGTACATTC CTGTGATTAA TAACAAGGTG TTTTTGCTCG ACGGTCCCAG GTGGGAATGG 
    GGTATTATCT TCGGCATGAT CATTGTGTAT GTCGGTGCTA CTGAGCTCTA CAAGTGGCTC 
    AAAAGAATTT GGATCCGAAG ACATGCCCCC CCTTCCAAGG GACCTACCGA CAAGACCCTT 
    AGGATGGAGA GTACCATTGC TCCTCCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_566813.1
    CDS1..3273
    Translation

    Target ORF information:

    RefSeq Version XM_566813.1
    Organism Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
    Definition Cryptococcus neoformans var. neoformans JEC21 hypothetical protein (CNA05130), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_566813.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCTTCTG AGAAAGGACA GTCAAACACA AACGAGAAAC AACTTATTAA CCGCGCCGAC 
    ACTGGCAAGA CTGCAGTGTC AGACTCTCCT CTCCCTTTCA AACCCCATAC CGCTCTCTCT 
    GGCAAGATCC TCGAGGCTTT AGGGAGTGAT GCAGCTTCTG GTCTATCAGA TGAGGAAGTA 
    TCAAGGAGAC TCCAACAGTA TGGTCCCAAT AGGCTGAAGC CCCCAAAGAG ACCCAGTATT 
    CTCAAGATCA TCGCTAGACA AGTGGGCAAT GCTATGACTC TTATCCTCAT CGCTGCCATG 
    GCAACTTCAT TGGGTACCAT GGACTGGATC AGCGGTGGCG TTATTGCGGC TCTGGTTATC 
    CTCAATGTAT CAGTGGGAGC CTACACAGAA TGGCAAGCCG AAAAGACCGT GGCCAGCCTC 
    GAGTCTGTGG GAGCTCCGCA AGCTACTGTA GTCCGAACTC GCAAAGGTTC TCGAGACCCT 
    ACCATCAGCA TTATCCCCGT CGAGGAAGTC GTACCCGGCG ACATCATTCA ACTCAAAAAT 
    GGTGATATCG TTCCTGCGGA CGGGAGAATC CTTGACGGGC ATCTGAGTAA CTTGGAAGCT 
    GATGAGGCTT TCCTCACTGG TGAAAGTCTG CCGGTTGCAA AGCAGACTGA GCCTATCGAT 
    GAAGAGGATT GTCCTGTTGG CGACCGTATT TGTATGGTCT TTTCTGGTTC CCAGATCACC 
    AAAGGTCGAG CTCGTGTTGT CATTACCAGC ACTGGTATGG GGACAGAGAT TGGAAAAATT 
    GCTCAAGCTC TTGAATCTAA AGCTCAAAAC AAGAATCGTG GATTTGCAGC TTTCTGGTGG 
    AAACTTAAAG TTATTTTGGG CGTCGAGGAG ACCACTCCTT TGCAAATGAA ACTTAACAAG 
    CTCGCATACT TCCTTTTGGC GTGTGCCCTC GTCATAGCCG TCATTGTTGT CGCCTCCACC 
    GGTTTTAATG ATGTTCCCCT CTCTATTGCC ACCTACGCTG TCGCTGCCGC CGTCTCCATC 
    CTTCCGGCCT CTTTGATTGC GGTTGTCAGT TTGACTTTGG CGCGTGCGTC AACTGATTTA 
    GCATCTCGAC ATGCTTTGGT CAGACGAATG GATGCTATTG AGGCTTTAGC TGGTGTTGAG 
    AATGTGTGCT CGGACAAGAC CGGTACCCTT ACTGTTGGCC GCATGGTAGT TCGCAAAGTC 
    TGGGTTCCTG CTCTCGACTG GCGCCCCAAT GATTTTGCTC CCCTCGACAC TAGTGGTGGT 
    CAAGCATACA GTTTTGAGAC CGGATCTGAC CCTTTCTATC CTCGTGGTGA AGTTCGGGCC 
    GATCCCCAAA AGATCTCTGG GACTGCAGAG ACCCTCGATC TCAAGCAACC CCGTGATCAA 
    TCTGACTCTT CCTCTTCCGA CACTGACCCC GAGGAGCGAG ATGTAGAGTA TCAAGAACGA 
    GTCATCCATG TGGAAGAAAT GGAGAACAAC CTTCGAGATC TTGCTCTCTG TATTTCGCTC 
    TGTAATCAAG CAACTCTCAG TCGTCCCGTA GACCAAGACG GCCAATGGGA AGCAAATGGT 
    GATCCTACCG AAACGGCCCT TCAAGTTGCT GCCCACAAGC TTGGTCATGG CAAGCCTTTT 
    CTTACTCATG TGGCCAAGCC AAGCCATCGT GCGGATTCTA TCCGATCTGG TCAGAGTTCT 
    CGTCCCCTTG TCGCTGGTAT TCGTGGGCAC TTTGTTCAGA TCATTGAGCA TCCTTTCGAT 
    TCCACCGTCA AACGGATGTC TATCGCCTAT AAATTTGTAG GCGAGGATCC TCAGGATTCC 
    CACGTCCTCT GTCTCCTTAA GGGTGCCATC GAGCGTGTCT TTGAGCGATG CACCAAGATC 
    CAAGGACAGC CAATCACCGA AGAGCATAAG AAGGATATCA TGATCAAAGT CGATGCTCTC 
    GCCGCTCAAG GTCTTCGGGT CCTCGCTCTT TGTGGAAAGC GACTTCCTGT CAGCATGGTC 
    GACGAAGTCA AATCTACCCC TCGAGACGCA TTCGAAGCCG ATTTTCACTT CCTCGGTCTT 
    GCTGGTATCT TCGACCCACC CAGAAAGGAA TCTGCTGGCG CTGTTGCTGA TTGTTTCAGG 
    GCTGGTATCA CCCCTCGGAT GTTGACAGGC GATCACCCTG CTACCGCGAC AGCTATCGCT 
    CTCAACATTG GTATTCTTGA TAAGACTTAC TCAAAGACTT CCGTCATGAC AGGTCAGCAA 
    TTCGACTCTT TGAGCGAAGA CGAAATTGAT CAACTGCCCG AGTTGCCTCT TGTCGTTGCT 
    CGCTGCGCCC CCGAGACCAA GGTTCGAATG GTCGATGCCA TTCATCGACG AGGACAAAGC 
    ACAGTCATGA CTGGTGATGG CGTCAACGAC TCCCCCGCCC TCAAGCGTGC CGATGTGGGC 
    GTTGGCATGG GTACTGGTTC CGATGTTGCC AAGCAGTCAG CGCGTATCGT CCTCAGTGAT 
    GACAACTTCA GCACCATCAT TCGAGCTATT AGGAAGGGTC GTTCTGTCTT CAAGAATTTG 
    TCTAAATTCT TGCTCTACTT GCTTTCGGGT AACTTGGCTG AAATCATCGT TCTGATGATT 
    GGTCTCGCTT TCAAGGATGA GAATGGTCAG GCTGTGTTCC CCCTGTCACC TGTTGCCGCT 
    CTTTGGATCA ACACTCTCGC TGCCGGACCT CCAGCCCTTG CCCTAGGTCT TGAACCTACA 
    GCTATCGACG CCATGGAGCA GGGGCCCGAA GTATACCATC AAATCTTTAC CCTCGAATTT 
    TACATCGACC TGATCTTCTA CGGCTTCCTG ATGGGTTCCA TCAGTTTGGT CAACTTCGTC 
    ATTGTACTCT GGGGATACTA TCCTGGAGAT TTAGGTCGTT TTTGTAACGA AGATGATCCC 
    AGCATCTGTG ATCCCGTCTA TCAGGCTCGA GCTGCCTGTT TTGCCACCCT CGTTATTGTC 
    CTCATGATCC ATGCTTTGGA GTGTAAGCAC TTGAACAAAG GGTTGGCCCA AATCAATTTG 
    CGTGACAACA AGGTGTTGCT GTGGTGTGTC GTTGTCCTCA GTCTTTCCGC TTTCCCTGTC 
    GTGTACATTC CTGTGATTAA TAACAAGGTG TTTTTGCTCG ACGGTCCCAG GTGGGAATGG 
    GGTATTATCT TCGGCATGAT CATTGTGTAT GTCGGTGCTA CTGAGCTCTA CAAGTGGCTC 
    AAAAGAATTT GGATCCGAAG ACATGCCCCC CCTTCCAAGG GACCTACCGA CAAGACCCTT 
    AGGATGGAGA GTACCATTGC TCCTCCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CNA05130 ( XM_566813.2 ) cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21) -> XP_566813.1 Cryptococcus neoformans var. neoformans JEC21 enolase 1, putative (CNA05130), mRNA.
    Price & Availability↑
    CloneID OCn00157
    Clone ID Related Accession (Same CDS sequence) XM_566813.1 , XM_566813.2
    Accession Version XM_566813.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1523289600000
    Organism Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
    Product hypothetical protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_006670). On Apr 11, 2018 this sequence version replaced XM_566813.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCTTCTG AGAAAGGACA GTCAAACACA AACGAGAAAC AACTTATTAA CCGCGCCGAC 
    ACTGGCAAGA CTGCAGTGTC AGACTCTCCT CTCCCTTTCA AACCCCATAC CGCTCTCTCT 
    GGCAAGATCC TCGAGGCTTT AGGGAGTGAT GCAGCTTCTG GTCTATCAGA TGAGGAAGTA 
    TCAAGGAGAC TCCAACAGTA TGGTCCCAAT AGGCTGAAGC CCCCAAAGAG ACCCAGTATT 
    CTCAAGATCA TCGCTAGACA AGTGGGCAAT GCTATGACTC TTATCCTCAT CGCTGCCATG 
    GCAACTTCAT TGGGTACCAT GGACTGGATC AGCGGTGGCG TTATTGCGGC TCTGGTTATC 
    CTCAATGTAT CAGTGGGAGC CTACACAGAA TGGCAAGCCG AAAAGACCGT GGCCAGCCTC 
    GAGTCTGTGG GAGCTCCGCA AGCTACTGTA GTCCGAACTC GCAAAGGTTC TCGAGACCCT 
    ACCATCAGCA TTATCCCCGT CGAGGAAGTC GTACCCGGCG ACATCATTCA ACTCAAAAAT 
    GGTGATATCG TTCCTGCGGA CGGGAGAATC CTTGACGGGC ATCTGAGTAA CTTGGAAGCT 
    GATGAGGCTT TCCTCACTGG TGAAAGTCTG CCGGTTGCAA AGCAGACTGA GCCTATCGAT 
    GAAGAGGATT GTCCTGTTGG CGACCGTATT TGTATGGTCT TTTCTGGTTC CCAGATCACC 
    AAAGGTCGAG CTCGTGTTGT CATTACCAGC ACTGGTATGG GGACAGAGAT TGGAAAAATT 
    GCTCAAGCTC TTGAATCTAA AGCTCAAAAC AAGAATCGTG GATTTGCAGC TTTCTGGTGG 
    AAACTTAAAG TTATTTTGGG CGTCGAGGAG ACCACTCCTT TGCAAATGAA ACTTAACAAG 
    CTCGCATACT TCCTTTTGGC GTGTGCCCTC GTCATAGCCG TCATTGTTGT CGCCTCCACC 
    GGTTTTAATG ATGTTCCCCT CTCTATTGCC ACCTACGCTG TCGCTGCCGC CGTCTCCATC 
    CTTCCGGCCT CTTTGATTGC GGTTGTCAGT TTGACTTTGG CGCGTGCGTC AACTGATTTA 
    GCATCTCGAC ATGCTTTGGT CAGACGAATG GATGCTATTG AGGCTTTAGC TGGTGTTGAG 
    AATGTGTGCT CGGACAAGAC CGGTACCCTT ACTGTTGGCC GCATGGTAGT TCGCAAAGTC 
    TGGGTTCCTG CTCTCGACTG GCGCCCCAAT GATTTTGCTC CCCTCGACAC TAGTGGTGGT 
    CAAGCATACA GTTTTGAGAC CGGATCTGAC CCTTTCTATC CTCGTGGTGA AGTTCGGGCC 
    GATCCCCAAA AGATCTCTGG GACTGCAGAG ACCCTCGATC TCAAGCAACC CCGTGATCAA 
    TCTGACTCTT CCTCTTCCGA CACTGACCCC GAGGAGCGAG ATGTAGAGTA TCAAGAACGA 
    GTCATCCATG TGGAAGAAAT GGAGAACAAC CTTCGAGATC TTGCTCTCTG TATTTCGCTC 
    TGTAATCAAG CAACTCTCAG TCGTCCCGTA GACCAAGACG GCCAATGGGA AGCAAATGGT 
    GATCCTACCG AAACGGCCCT TCAAGTTGCT GCCCACAAGC TTGGTCATGG CAAGCCTTTT 
    CTTACTCATG TGGCCAAGCC AAGCCATCGT GCGGATTCTA TCCGATCTGG TCAGAGTTCT 
    CGTCCCCTTG TCGCTGGTAT TCGTGGGCAC TTTGTTCAGA TCATTGAGCA TCCTTTCGAT 
    TCCACCGTCA AACGGATGTC TATCGCCTAT AAATTTGTAG GCGAGGATCC TCAGGATTCC 
    CACGTCCTCT GTCTCCTTAA GGGTGCCATC GAGCGTGTCT TTGAGCGATG CACCAAGATC 
    CAAGGACAGC CAATCACCGA AGAGCATAAG AAGGATATCA TGATCAAAGT CGATGCTCTC 
    GCCGCTCAAG GTCTTCGGGT CCTCGCTCTT TGTGGAAAGC GACTTCCTGT CAGCATGGTC 
    GACGAAGTCA AATCTACCCC TCGAGACGCA TTCGAAGCCG ATTTTCACTT CCTCGGTCTT 
    GCTGGTATCT TCGACCCACC CAGAAAGGAA TCTGCTGGCG CTGTTGCTGA TTGTTTCAGG 
    GCTGGTATCA CCCCTCGGAT GTTGACAGGC GATCACCCTG CTACCGCGAC AGCTATCGCT 
    CTCAACATTG GTATTCTTGA TAAGACTTAC TCAAAGACTT CCGTCATGAC AGGTCAGCAA 
    TTCGACTCTT TGAGCGAAGA CGAAATTGAT CAACTGCCCG AGTTGCCTCT TGTCGTTGCT 
    CGCTGCGCCC CCGAGACCAA GGTTCGAATG GTCGATGCCA TTCATCGACG AGGACAAAGC 
    ACAGTCATGA CTGGTGATGG CGTCAACGAC TCCCCCGCCC TCAAGCGTGC CGATGTGGGC 
    GTTGGCATGG GTACTGGTTC CGATGTTGCC AAGCAGTCAG CGCGTATCGT CCTCAGTGAT 
    GACAACTTCA GCACCATCAT TCGAGCTATT AGGAAGGGTC GTTCTGTCTT CAAGAATTTG 
    TCTAAATTCT TGCTCTACTT GCTTTCGGGT AACTTGGCTG AAATCATCGT TCTGATGATT 
    GGTCTCGCTT TCAAGGATGA GAATGGTCAG GCTGTGTTCC CCCTGTCACC TGTTGCCGCT 
    CTTTGGATCA ACACTCTCGC TGCCGGACCT CCAGCCCTTG CCCTAGGTCT TGAACCTACA 
    GCTATCGACG CCATGGAGCA GGGGCCCGAA GTATACCATC AAATCTTTAC CCTCGAATTT 
    TACATCGACC TGATCTTCTA CGGCTTCCTG ATGGGTTCCA TCAGTTTGGT CAACTTCGTC 
    ATTGTACTCT GGGGATACTA TCCTGGAGAT TTAGGTCGTT TTTGTAACGA AGATGATCCC 
    AGCATCTGTG ATCCCGTCTA TCAGGCTCGA GCTGCCTGTT TTGCCACCCT CGTTATTGTC 
    CTCATGATCC ATGCTTTGGA GTGTAAGCAC TTGAACAAAG GGTTGGCCCA AATCAATTTG 
    CGTGACAACA AGGTGTTGCT GTGGTGTGTC GTTGTCCTCA GTCTTTCCGC TTTCCCTGTC 
    GTGTACATTC CTGTGATTAA TAACAAGGTG TTTTTGCTCG ACGGTCCCAG GTGGGAATGG 
    GGTATTATCT TCGGCATGAT CATTGTGTAT GTCGGTGCTA CTGAGCTCTA CAAGTGGCTC 
    AAAAGAATTT GGATCCGAAG ACATGCCCCC CCTTCCAAGG GACCTACCGA CAAGACCCTT 
    AGGATGGAGA GTACCATTGC TCCTCCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_566813.1
    CDS25..3297
    Translation

    Target ORF information:

    RefSeq Version XM_566813.2
    Organism Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
    Definition Cryptococcus neoformans var. neoformans JEC21 enolase 1, putative (CNA05130), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_566813.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCTTCTG AGAAAGGACA GTCAAACACA AACGAGAAAC AACTTATTAA CCGCGCCGAC 
    ACTGGCAAGA CTGCAGTGTC AGACTCTCCT CTCCCTTTCA AACCCCATAC CGCTCTCTCT 
    GGCAAGATCC TCGAGGCTTT AGGGAGTGAT GCAGCTTCTG GTCTATCAGA TGAGGAAGTA 
    TCAAGGAGAC TCCAACAGTA TGGTCCCAAT AGGCTGAAGC CCCCAAAGAG ACCCAGTATT 
    CTCAAGATCA TCGCTAGACA AGTGGGCAAT GCTATGACTC TTATCCTCAT CGCTGCCATG 
    GCAACTTCAT TGGGTACCAT GGACTGGATC AGCGGTGGCG TTATTGCGGC TCTGGTTATC 
    CTCAATGTAT CAGTGGGAGC CTACACAGAA TGGCAAGCCG AAAAGACCGT GGCCAGCCTC 
    GAGTCTGTGG GAGCTCCGCA AGCTACTGTA GTCCGAACTC GCAAAGGTTC TCGAGACCCT 
    ACCATCAGCA TTATCCCCGT CGAGGAAGTC GTACCCGGCG ACATCATTCA ACTCAAAAAT 
    GGTGATATCG TTCCTGCGGA CGGGAGAATC CTTGACGGGC ATCTGAGTAA CTTGGAAGCT 
    GATGAGGCTT TCCTCACTGG TGAAAGTCTG CCGGTTGCAA AGCAGACTGA GCCTATCGAT 
    GAAGAGGATT GTCCTGTTGG CGACCGTATT TGTATGGTCT TTTCTGGTTC CCAGATCACC 
    AAAGGTCGAG CTCGTGTTGT CATTACCAGC ACTGGTATGG GGACAGAGAT TGGAAAAATT 
    GCTCAAGCTC TTGAATCTAA AGCTCAAAAC AAGAATCGTG GATTTGCAGC TTTCTGGTGG 
    AAACTTAAAG TTATTTTGGG CGTCGAGGAG ACCACTCCTT TGCAAATGAA ACTTAACAAG 
    CTCGCATACT TCCTTTTGGC GTGTGCCCTC GTCATAGCCG TCATTGTTGT CGCCTCCACC 
    GGTTTTAATG ATGTTCCCCT CTCTATTGCC ACCTACGCTG TCGCTGCCGC CGTCTCCATC 
    CTTCCGGCCT CTTTGATTGC GGTTGTCAGT TTGACTTTGG CGCGTGCGTC AACTGATTTA 
    GCATCTCGAC ATGCTTTGGT CAGACGAATG GATGCTATTG AGGCTTTAGC TGGTGTTGAG 
    AATGTGTGCT CGGACAAGAC CGGTACCCTT ACTGTTGGCC GCATGGTAGT TCGCAAAGTC 
    TGGGTTCCTG CTCTCGACTG GCGCCCCAAT GATTTTGCTC CCCTCGACAC TAGTGGTGGT 
    CAAGCATACA GTTTTGAGAC CGGATCTGAC CCTTTCTATC CTCGTGGTGA AGTTCGGGCC 
    GATCCCCAAA AGATCTCTGG GACTGCAGAG ACCCTCGATC TCAAGCAACC CCGTGATCAA 
    TCTGACTCTT CCTCTTCCGA CACTGACCCC GAGGAGCGAG ATGTAGAGTA TCAAGAACGA 
    GTCATCCATG TGGAAGAAAT GGAGAACAAC CTTCGAGATC TTGCTCTCTG TATTTCGCTC 
    TGTAATCAAG CAACTCTCAG TCGTCCCGTA GACCAAGACG GCCAATGGGA AGCAAATGGT 
    GATCCTACCG AAACGGCCCT TCAAGTTGCT GCCCACAAGC TTGGTCATGG CAAGCCTTTT 
    CTTACTCATG TGGCCAAGCC AAGCCATCGT GCGGATTCTA TCCGATCTGG TCAGAGTTCT 
    CGTCCCCTTG TCGCTGGTAT TCGTGGGCAC TTTGTTCAGA TCATTGAGCA TCCTTTCGAT 
    TCCACCGTCA AACGGATGTC TATCGCCTAT AAATTTGTAG GCGAGGATCC TCAGGATTCC 
    CACGTCCTCT GTCTCCTTAA GGGTGCCATC GAGCGTGTCT TTGAGCGATG CACCAAGATC 
    CAAGGACAGC CAATCACCGA AGAGCATAAG AAGGATATCA TGATCAAAGT CGATGCTCTC 
    GCCGCTCAAG GTCTTCGGGT CCTCGCTCTT TGTGGAAAGC GACTTCCTGT CAGCATGGTC 
    GACGAAGTCA AATCTACCCC TCGAGACGCA TTCGAAGCCG ATTTTCACTT CCTCGGTCTT 
    GCTGGTATCT TCGACCCACC CAGAAAGGAA TCTGCTGGCG CTGTTGCTGA TTGTTTCAGG 
    GCTGGTATCA CCCCTCGGAT GTTGACAGGC GATCACCCTG CTACCGCGAC AGCTATCGCT 
    CTCAACATTG GTATTCTTGA TAAGACTTAC TCAAAGACTT CCGTCATGAC AGGTCAGCAA 
    TTCGACTCTT TGAGCGAAGA CGAAATTGAT CAACTGCCCG AGTTGCCTCT TGTCGTTGCT 
    CGCTGCGCCC CCGAGACCAA GGTTCGAATG GTCGATGCCA TTCATCGACG AGGACAAAGC 
    ACAGTCATGA CTGGTGATGG CGTCAACGAC TCCCCCGCCC TCAAGCGTGC CGATGTGGGC 
    GTTGGCATGG GTACTGGTTC CGATGTTGCC AAGCAGTCAG CGCGTATCGT CCTCAGTGAT 
    GACAACTTCA GCACCATCAT TCGAGCTATT AGGAAGGGTC GTTCTGTCTT CAAGAATTTG 
    TCTAAATTCT TGCTCTACTT GCTTTCGGGT AACTTGGCTG AAATCATCGT TCTGATGATT 
    GGTCTCGCTT TCAAGGATGA GAATGGTCAG GCTGTGTTCC CCCTGTCACC TGTTGCCGCT 
    CTTTGGATCA ACACTCTCGC TGCCGGACCT CCAGCCCTTG CCCTAGGTCT TGAACCTACA 
    GCTATCGACG CCATGGAGCA GGGGCCCGAA GTATACCATC AAATCTTTAC CCTCGAATTT 
    TACATCGACC TGATCTTCTA CGGCTTCCTG ATGGGTTCCA TCAGTTTGGT CAACTTCGTC 
    ATTGTACTCT GGGGATACTA TCCTGGAGAT TTAGGTCGTT TTTGTAACGA AGATGATCCC 
    AGCATCTGTG ATCCCGTCTA TCAGGCTCGA GCTGCCTGTT TTGCCACCCT CGTTATTGTC 
    CTCATGATCC ATGCTTTGGA GTGTAAGCAC TTGAACAAAG GGTTGGCCCA AATCAATTTG 
    CGTGACAACA AGGTGTTGCT GTGGTGTGTC GTTGTCCTCA GTCTTTCCGC TTTCCCTGTC 
    GTGTACATTC CTGTGATTAA TAACAAGGTG TTTTTGCTCG ACGGTCCCAG GTGGGAATGG 
    GGTATTATCT TCGGCATGAT CATTGTGTAT GTCGGTGCTA CTGAGCTCTA CAAGTGGCTC 
    AAAAGAATTT GGATCCGAAG ACATGCCCCC CCTTCCAAGG GACCTACCGA CAAGACCCTT 
    AGGATGGAGA GTACCATTGC TCCTCCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.
    Science (New York, N.Y.)307(5713)1321-4(2005 Feb)
    Loftus BJ,Fung E,Roncaglia P,Rowley D,Amedeo P,Bruno D,Vamathevan J,Miranda M,Anderson IJ,Fraser JA,Allen JE,Bosdet IE,Brent MR,Chiu R,Doering TL,Donlin MJ,D'Souza CA,Fox DS,Grinberg V,Fu J,Fukushima M,Haas BJ,Huang JC,Janbon G,Jones SJ,Koo HL,Krzywinski MI,Kwon-Chung JK,Lengeler KB,Maiti R,Marra MA,Marra RE,Mathewson CA,Mitchell TG,Pertea M,Riggs FR,Salzberg SL,Schein JE,Shvartsbeyn A,Shin H,Shumway M,Specht CA,Suh BB,Tenney A,Utterback TR,Wickes BL,Wortman JR,Wye NH,Kronstad JW,Lodge JK,Heitman J,Davis RW,Fraser CM,Hyman RW


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