GUCY2C cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUCY2C
Entrez Gene ID	103218671
Full Name	guanylate cyclase 2C
Gene Type	protein-coding
Organism	Chlorocebus sabaeus(green monkey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007967738.1	XP_007965929.1	heat-stable enterotoxin receptor isoform X1
XM_007967739.1	XP_007965930.1	heat-stable enterotoxin receptor isoform X2

Pathways from BioSystems
Gene Ontology

The following GUCY2C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCY2C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCh96430	XM_007967738.1 Latest version!	Chlorocebus sabaeus guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCh96431	XM_007967739.1 Latest version!	Chlorocebus sabaeus guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUCY2C ( XM_007967738.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007965929.1 Chlorocebus sabaeus guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCh96430
Clone ID Related Accession (Same CDS sequence)	XM_007967738.1
Accession Version	XM_007967738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	heat-stable enterotoxin receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735253.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGT TGCTGTTGGA CTTGGTTTTG TGGTCACTGC TCTTCCAGCC TGGGTGGCTG 
TACCTGACTT CCCGGGTGAG TCAGAACTGC CACAATGGCA GCTATGAAAT CAGCGTCCTG 
ATGATGGACA ACTCAGCCTT TGCAGAGCCC CTGGAAAACG TGGAAGATGC AGTGAATGAG 
GGGCTGGAAA TAGTGAGAGG ACGTCTGCAA AACGCTGGCC TAAATGTGAC TGTGAATGCT 
TCTTTCATGT ATTCGGATGG TCTGATTCAT AACTCCGGCG ACTGCCGGAG CAGCACCTGT 
GAAGGCCTTG ACCTACTCAG GAAAATTTCA AATGCAAAAC GGATGGGCTG TGTCCTCATG 
GGGCCCTCAT GTACATACTC CACCTTCCAG ATGTACCTTG ACACGGAATT GAGCTACCCC 
ATGATCTCAG CTGGAAGTTT TGGATTGTCA TGTGACTATA AAGAAACCTT AACCCGGCTG 
ATGTCTCCAG CTAGAAAGTT GACATACTTC TTGGTTAACT TTTGGAAAAC CAATGATCTA 
CCCTTCAAAA CTTATTCCTG GAGCACTTCG TATGTTTACA AGAATGGTAC GGAGTCCGAG 
GACTGTTTCT GGTACCTTAA CGCTCTGGAG GCCAGTGTTT CCTATTTCTC CCACGAACTC 
AGTTTTAAGT TGGTGTTAAG ACAAGATAAG GAGTTTCAGG ATATCTTAAT GGACCACAAC 
AGGAAAAGCA ATGTGATTGT TATGTGTGGT GGTCCAGAGT TCCTCTACAA GTTGAAGGGT 
GACCGAGCAG TGGCTGAAGA CATTGTCATT ATTCTAGTGG ATCTCTTCAA TGACCAGTAC 
TTTGAGGACA ATGTCACAGC CCCTGACTAT ATGAAAAATG TCCTTGTTCT GACGCAGTCT 
CCTGGGAATT CCCTCCTAAA TAGCTCTTTC TCCAGGAATC TATCCCCAAC AAAACGAGAC 
TTTGCTCTTG CCTATTTGAA TGGAATCCTG CTCTTTGGAC ATGTGCTGAA GATATTTCTT 
GAAAATGGAG AAAATATTAC CACCCCCAAA TTTGCTCATG CTTTCAGGAA TCTCACTTTT 
GAAGGGTATA ATGGTCCAGT GACCTTGGAT GACTGGGGAG ATGTGGACAG TACCATGGTG 
CTTCTGTATA CGTCTGTGAA CACCAAGAAA TACAAGGTTC TTTTGACCTA TGATACCCAC 
GTAAATCAGA CCCACCCTGT GGATATGAGC CCCACATTCA CTTGGAAGAA CTCTAAACTT 
CCTAATGATA TTACAGACCG GGGCCCTCAG ATCCTGATGA TTGCAGTCTT CACCCTCACC 
GGAGCTGTGG TGCTGCTCCT GCTTGTCGCT CTCCTGATGC TCAGAAAATA CAAAAAAAAT 
TATGAACTTC GTCAGAAAAA ATGGTCCCAC ATTCCTCCTG AAAATATCTT TCCTCTGGAG 
ACCAATGAGA CCAATCACGT TAGCCTGAAG ATCGATGATG ACAAAAGACG AGATACAATC 
CAGAGACTAC GACAGTGCAA ATACGACAAA AAGCGAGTGA TTCTCAAAGA TCTCAAGCAC 
AATGATGGTA ATTTCACTGA AAAACAGAAG ATAGAATTGA ACAAGTTGCT TCAGATTGAC 
TATTACAACC TGACCAAGTT CTATGGCACA GTGAAACTTG ATACCATGAT CTTCGGGGTG 
ATAGAATACT GTGAGAGAGG ATCCCTCCGG GAAGTTTTAA ATGACACAAT TTCCTACCCT 
GATGGCACAT TCATGGATTG GGAGTTTAAG ATCTCTGTCT TGTATGACAT TGCTAAGGGA 
ATGTCATATC TGCACTCCAG TAAGACAGAA GTCCATGGTC GTCTGAAATC TACCAACTGC 
GTAGTGGACA GTAGAATGGT GGTGAAGATC ACTGATTTTG GCTGCAATTC CATTTTACCT 
CCAAAAAAAG ACCTGTGGAC AGCTCCAGAG CACCTCCGCC AAGCCAACGT CTCTCAGAAA 
GGAGATGTGT ACAGCTATGG GATCATCGCA CAGGAGATCA TCCTGCGGAA AGAAACCTTC 
TACACTTCTA GCTGTCGAGA CCGGAACGAG AAGATTTTCA GAGTGGAAAA TTCCAATGGA 
ATGAAACCCT TCCGCCCAGA TTTATTCTTG GAAACGGCAG AGGAAAAAGA GCTAGAAGTG 
TACCTACTTG TAAAAAGCTG TTGGGAAGAA GATCCAGAAA AGAGACCAGA TTTCAAAAAA 
ATTGAGACTA CACTTGCCAA GATATTTGGA CTTTTTCATG ACCAAAAAAA TGAAAGCTAT 
ATGGATACCT TGATCCGACG TCTACAGCTA TATTCTCGAA ACCTGGAACA TCTGGTAGAG 
GAAAGGACAC AGCTATACAA GGCAGAGAGG GACAGGGCTG ACAGACTTAA CTTTATGTTG 
CTTCCAAGGC TAGTGGTAAA GTCTCTGAAG GAGAAAGGCT TTGTAGAACC GGAACTATAT 
GAGGAAGTTA CAATCTACTT CAGTGACATT GTAGGTTTCA CTACTATCTG CAAATACAGC 
ACCCCCATGG AAGTGGTGGA CATGCTTAAT GACATCTATA AGAGTTTTGA CCACATTGTT 
GATCATCATG ATGTCTACAA GGTGGAAACC ATTGGCGATG CCTACATGGT GGCTAGTGGT 
TTGCCTAAGA GAAATGGCAA TCGGCATGCA ATAGACATTG CCAAGATGGC CTTGGAAATC 
CTCAGCTTCA TGGGGACCTT TGAGCTGGAG CATCTTCCTG GCCTCCCAAT ATGGATTCGC 
ATTGGCGTTC ACTCTGGTCC CTGCGCTGCT GGAGTTGTGG GAATCAAGAT GCCTCGTTAT 
TGTCTATTCG GAGATACAGT CAACACAGCC TCTAGGATGG AATCCACTGG CCTCCCTTTG 
AGAATTCATG TGAGTGGCTC CACCATAGCC ATTCTGAAGA GAACTGAGTG CCAGTTCCTG 
TATGAAGTGA GAGGAGAAAC GTACTTAAAG GGAAGAGGAA ATGAGACTAC CTACTGGCTG 
ACCGGGATGA AGGACCAGAA ATTCAACCTG CCAACCCCTC CTACTGTGGA GAATCAACAG 
CGTTTGCAAG CAGAATTTTC AGATATGATT GCCAACTCTT TACAGAAAAG ACAGGCAGCA 
GGGATAAGAA GCCAAAAACC CAGACGAGTA GCCAGCTATA AAAAAGGCAC TCTGGAATAC 
TTGCAACTGA ATACCACAGA CAAGGAGAGC ACCTATTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007965929.1
CDS	574..3795
Translation	MKTLLLDLVLWSLLFQPGWLYLTSRVSQNCHNGSYEISVLMMDNSAFAEPLENVEDAVNEGLEIVRGRLQNAGLNVTVNASFMYSDGLIHNSGDCRSSTCEGLDLLRKISNAKRMGCVLMGPSCTYSTFQMYLDTELSYPMISAGSFGLSCDYKETLTRLMSPARKLTYFLVNFWKTNDLPFKTYSWSTSYVYKNGTESEDCFWYLNALEASVSYFSHELSFKLVLRQDKEFQDILMDHNRKSNVIVMCGGPEFLYKLKGDRAVAEDIVIILVDLFNDQYFEDNVTAPDYMKNVLVLTQSPGNSLLNSSFSRNLSPTKRDFALAYLNGILLFGHVLKIFLENGENITTPKFAHAFRNLTFEGYNGPVTLDDWGDVDSTMVLLYTSVNTKKYKVLLTYDTHVNQTHPVDMSPTFTWKNSKLPNDITDRGPQILMIAVFTLTGAVVLLLLVALLMLRKYKKNYELRQKKWSHIPPENIFPLETNETNHVSLKIDDDKRRDTIQRLRQCKYDKKRVILKDLKHNDGNFTEKQKIELNKLLQIDYYNLTKFYGTVKLDTMIFGVIEYCERGSLREVLNDTISYPDGTFMDWEFKISVLYDIAKGMSYLHSSKTEVHGRLKSTNCVVDSRMVVKITDFGCNSILPPKKDLWTAPEHLRQANVSQKGDVYSYGIIAQEIILRKETFYTSSCRDRNEKIFRVENSNGMKPFRPDLFLETAEEKELEVYLLVKSCWEEDPEKRPDFKKIETTLAKIFGLFHDQKNESYMDTLIRRLQLYSRNLEHLVEERTQLYKAERDRADRLNFMLLPRLVVKSLKEKGFVEPELYEEVTIYFSDIVGFTTICKYSTPMEVVDMLNDIYKSFDHIVDHHDVYKVETIGDAYMVASGLPKRNGNRHAIDIAKMALEILSFMGTFELEHLPGLPIWIRIGVHSGPCAAGVVGIKMPRYCLFGDTVNTASRMESTGLPLRIHVSGSTIAILKRTECQFLYEVRGETYLKGRGNETTYWLTGMKDQKFNLPTPPTVENQQRLQAEFSDMIANSLQKRQAAGIRSQKPRRVASYKKGTLEYLQLNTTDKESTYF

Target ORF information:

RefSeq Version	XM_007967738.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007967738.1

ORF Insert Sequence:

ATGAAGACGT TGCTGTTGGA CTTGGTTTTG TGGTCACTGC TCTTCCAGCC TGGGTGGCTG 
TACCTGACTT CCCGGGTGAG TCAGAACTGC CACAATGGCA GCTATGAAAT CAGCGTCCTG 
ATGATGGACA ACTCAGCCTT TGCAGAGCCC CTGGAAAACG TGGAAGATGC AGTGAATGAG 
GGGCTGGAAA TAGTGAGAGG ACGTCTGCAA AACGCTGGCC TAAATGTGAC TGTGAATGCT 
TCTTTCATGT ATTCGGATGG TCTGATTCAT AACTCCGGCG ACTGCCGGAG CAGCACCTGT 
GAAGGCCTTG ACCTACTCAG GAAAATTTCA AATGCAAAAC GGATGGGCTG TGTCCTCATG 
GGGCCCTCAT GTACATACTC CACCTTCCAG ATGTACCTTG ACACGGAATT GAGCTACCCC 
ATGATCTCAG CTGGAAGTTT TGGATTGTCA TGTGACTATA AAGAAACCTT AACCCGGCTG 
ATGTCTCCAG CTAGAAAGTT GACATACTTC TTGGTTAACT TTTGGAAAAC CAATGATCTA 
CCCTTCAAAA CTTATTCCTG GAGCACTTCG TATGTTTACA AGAATGGTAC GGAGTCCGAG 
GACTGTTTCT GGTACCTTAA CGCTCTGGAG GCCAGTGTTT CCTATTTCTC CCACGAACTC 
AGTTTTAAGT TGGTGTTAAG ACAAGATAAG GAGTTTCAGG ATATCTTAAT GGACCACAAC 
AGGAAAAGCA ATGTGATTGT TATGTGTGGT GGTCCAGAGT TCCTCTACAA GTTGAAGGGT 
GACCGAGCAG TGGCTGAAGA CATTGTCATT ATTCTAGTGG ATCTCTTCAA TGACCAGTAC 
TTTGAGGACA ATGTCACAGC CCCTGACTAT ATGAAAAATG TCCTTGTTCT GACGCAGTCT 
CCTGGGAATT CCCTCCTAAA TAGCTCTTTC TCCAGGAATC TATCCCCAAC AAAACGAGAC 
TTTGCTCTTG CCTATTTGAA TGGAATCCTG CTCTTTGGAC ATGTGCTGAA GATATTTCTT 
GAAAATGGAG AAAATATTAC CACCCCCAAA TTTGCTCATG CTTTCAGGAA TCTCACTTTT 
GAAGGGTATA ATGGTCCAGT GACCTTGGAT GACTGGGGAG ATGTGGACAG TACCATGGTG 
CTTCTGTATA CGTCTGTGAA CACCAAGAAA TACAAGGTTC TTTTGACCTA TGATACCCAC 
GTAAATCAGA CCCACCCTGT GGATATGAGC CCCACATTCA CTTGGAAGAA CTCTAAACTT 
CCTAATGATA TTACAGACCG GGGCCCTCAG ATCCTGATGA TTGCAGTCTT CACCCTCACC 
GGAGCTGTGG TGCTGCTCCT GCTTGTCGCT CTCCTGATGC TCAGAAAATA CAAAAAAAAT 
TATGAACTTC GTCAGAAAAA ATGGTCCCAC ATTCCTCCTG AAAATATCTT TCCTCTGGAG 
ACCAATGAGA CCAATCACGT TAGCCTGAAG ATCGATGATG ACAAAAGACG AGATACAATC 
CAGAGACTAC GACAGTGCAA ATACGACAAA AAGCGAGTGA TTCTCAAAGA TCTCAAGCAC 
AATGATGGTA ATTTCACTGA AAAACAGAAG ATAGAATTGA ACAAGTTGCT TCAGATTGAC 
TATTACAACC TGACCAAGTT CTATGGCACA GTGAAACTTG ATACCATGAT CTTCGGGGTG 
ATAGAATACT GTGAGAGAGG ATCCCTCCGG GAAGTTTTAA ATGACACAAT TTCCTACCCT 
GATGGCACAT TCATGGATTG GGAGTTTAAG ATCTCTGTCT TGTATGACAT TGCTAAGGGA 
ATGTCATATC TGCACTCCAG TAAGACAGAA GTCCATGGTC GTCTGAAATC TACCAACTGC 
GTAGTGGACA GTAGAATGGT GGTGAAGATC ACTGATTTTG GCTGCAATTC CATTTTACCT 
CCAAAAAAAG ACCTGTGGAC AGCTCCAGAG CACCTCCGCC AAGCCAACGT CTCTCAGAAA 
GGAGATGTGT ACAGCTATGG GATCATCGCA CAGGAGATCA TCCTGCGGAA AGAAACCTTC 
TACACTTCTA GCTGTCGAGA CCGGAACGAG AAGATTTTCA GAGTGGAAAA TTCCAATGGA 
ATGAAACCCT TCCGCCCAGA TTTATTCTTG GAAACGGCAG AGGAAAAAGA GCTAGAAGTG 
TACCTACTTG TAAAAAGCTG TTGGGAAGAA GATCCAGAAA AGAGACCAGA TTTCAAAAAA 
ATTGAGACTA CACTTGCCAA GATATTTGGA CTTTTTCATG ACCAAAAAAA TGAAAGCTAT 
ATGGATACCT TGATCCGACG TCTACAGCTA TATTCTCGAA ACCTGGAACA TCTGGTAGAG 
GAAAGGACAC AGCTATACAA GGCAGAGAGG GACAGGGCTG ACAGACTTAA CTTTATGTTG 
CTTCCAAGGC TAGTGGTAAA GTCTCTGAAG GAGAAAGGCT TTGTAGAACC GGAACTATAT 
GAGGAAGTTA CAATCTACTT CAGTGACATT GTAGGTTTCA CTACTATCTG CAAATACAGC 
ACCCCCATGG AAGTGGTGGA CATGCTTAAT GACATCTATA AGAGTTTTGA CCACATTGTT 
GATCATCATG ATGTCTACAA GGTGGAAACC ATTGGCGATG CCTACATGGT GGCTAGTGGT 
TTGCCTAAGA GAAATGGCAA TCGGCATGCA ATAGACATTG CCAAGATGGC CTTGGAAATC 
CTCAGCTTCA TGGGGACCTT TGAGCTGGAG CATCTTCCTG GCCTCCCAAT ATGGATTCGC 
ATTGGCGTTC ACTCTGGTCC CTGCGCTGCT GGAGTTGTGG GAATCAAGAT GCCTCGTTAT 
TGTCTATTCG GAGATACAGT CAACACAGCC TCTAGGATGG AATCCACTGG CCTCCCTTTG 
AGAATTCATG TGAGTGGCTC CACCATAGCC ATTCTGAAGA GAACTGAGTG CCAGTTCCTG 
TATGAAGTGA GAGGAGAAAC GTACTTAAAG GGAAGAGGAA ATGAGACTAC CTACTGGCTG 
ACCGGGATGA AGGACCAGAA ATTCAACCTG CCAACCCCTC CTACTGTGGA GAATCAACAG 
CGTTTGCAAG CAGAATTTTC AGATATGATT GCCAACTCTT TACAGAAAAG ACAGGCAGCA 
GGGATAAGAA GCCAAAAACC CAGACGAGTA GCCAGCTATA AAAAAGGCAC TCTGGAATAC 
TTGCAACTGA ATACCACAGA CAAGGAGAGC ACCTATTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY2C ( XM_007967739.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007965930.1 Chlorocebus sabaeus guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCh96431
Clone ID Related Accession (Same CDS sequence)	XM_007967739.1
Accession Version	XM_007967739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	heat-stable enterotoxin receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTCGG ATGGTCTGAT TCATAACTCC GGCGACTGCC GGAGCAGCAC CTGTGAAGGC 
CTTGACCTAC TCAGGAAAAT TTCAAATGCA AAACGGATGG GCTGTGTCCT CATGGGGCCC 
TCATGTACAT ACTCCACCTT CCAGATGTAC CTTGACACGG AATTGAGCTA CCCCATGATC 
TCAGCTGGAA GTTTTGGATT GTCATGTGAC TATAAAGAAA CCTTAACCCG GCTGATGTCT 
CCAGCTAGAA AGTTGACATA CTTCTTGGTT AACTTTTGGA AAACCAATGA TCTACCCTTC 
AAAACTTATT CCTGGAGCAC TTCGTATGTT TACAAGAATG GTACGGAGTC CGAGGACTGT 
TTCTGGTACC TTAACGCTCT GGAGGCCAGT GTTTCCTATT TCTCCCACGA ACTCAGTTTT 
AAGTTGGTGT TAAGACAAGA TAAGGAGTTT CAGGATATCT TAATGGACCA CAACAGGAAA 
AGCAATGTGA TTGTTATGTG TGGTGGTCCA GAGTTCCTCT ACAAGTTGAA GGGTGACCGA 
GCAGTGGCTG AAGACATTGT CATTATTCTA GTGGATCTCT TCAATGACCA GTACTTTGAG 
GACAATGTCA CAGCCCCTGA CTATATGAAA AATGTCCTTG TTCTGACGCA GTCTCCTGGG 
AATTCCCTCC TAAATAGCTC TTTCTCCAGG AATCTATCCC CAACAAAACG AGACTTTGCT 
CTTGCCTATT TGAATGGAAT CCTGCTCTTT GGACATGTGC TGAAGATATT TCTTGAAAAT 
GGAGAAAATA TTACCACCCC CAAATTTGCT CATGCTTTCA GGAATCTCAC TTTTGAAGGG 
TATAATGGTC CAGTGACCTT GGATGACTGG GGAGATGTGG ACAGTACCAT GGTGCTTCTG 
TATACGTCTG TGAACACCAA GAAATACAAG GTTCTTTTGA CCTATGATAC CCACGTAAAT 
CAGACCCACC CTGTGGATAT GAGCCCCACA TTCACTTGGA AGAACTCTAA ACTTCCTAAT 
GATATTACAG ACCGGGGCCC TCAGATCCTG ATGATTGCAG TCTTCACCCT CACCGGAGCT 
GTGGTGCTGC TCCTGCTTGT CGCTCTCCTG ATGCTCAGAA AATACAAAAA AAATTATGAA 
CTTCGTCAGA AAAAATGGTC CCACATTCCT CCTGAAAATA TCTTTCCTCT GGAGACCAAT 
GAGACCAATC ACGTTAGCCT GAAGATCGAT GATGACAAAA GACGAGATAC AATCCAGAGA 
CTACGACAGT GCAAATACGA CAAAAAGCGA GTGATTCTCA AAGATCTCAA GCACAATGAT 
GGTAATTTCA CTGAAAAACA GAAGATAGAA TTGAACAAGT TGCTTCAGAT TGACTATTAC 
AACCTGACCA AGTTCTATGG CACAGTGAAA CTTGATACCA TGATCTTCGG GGTGATAGAA 
TACTGTGAGA GAGGATCCCT CCGGGAAGTT TTAAATGACA CAATTTCCTA CCCTGATGGC 
ACATTCATGG ATTGGGAGTT TAAGATCTCT GTCTTGTATG ACATTGCTAA GGGAATGTCA 
TATCTGCACT CCAGTAAGAC AGAAGTCCAT GGTCGTCTGA AATCTACCAA CTGCGTAGTG 
GACAGTAGAA TGGTGGTGAA GATCACTGAT TTTGGCTGCA ATTCCATTTT ACCTCCAAAA 
AAAGACCTGT GGACAGCTCC AGAGCACCTC CGCCAAGCCA ACGTCTCTCA GAAAGGAGAT 
GTGTACAGCT ATGGGATCAT CGCACAGGAG ATCATCCTGC GGAAAGAAAC CTTCTACACT 
TCTAGCTGTC GAGACCGGAA CGAGAAGATT TTCAGAGTGG AAAATTCCAA TGGAATGAAA 
CCCTTCCGCC CAGATTTATT CTTGGAAACG GCAGAGGAAA AAGAGCTAGA AGTGTACCTA 
CTTGTAAAAA GCTGTTGGGA AGAAGATCCA GAAAAGAGAC CAGATTTCAA AAAAATTGAG 
ACTACACTTG CCAAGATATT TGGACTTTTT CATGACCAAA AAAATGAAAG CTATATGGAT 
ACCTTGATCC GACGTCTACA GCTATATTCT CGAAACCTGG AACATCTGGT AGAGGAAAGG 
ACACAGCTAT ACAAGGCAGA GAGGGACAGG GCTGACAGAC TTAACTTTAT GTTGCTTCCA 
AGGCTAGTGG TAAAGTCTCT GAAGGAGAAA GGCTTTGTAG AACCGGAACT ATATGAGGAA 
GTTACAATCT ACTTCAGTGA CATTGTAGGT TTCACTACTA TCTGCAAATA CAGCACCCCC 
ATGGAAGTGG TGGACATGCT TAATGACATC TATAAGAGTT TTGACCACAT TGTTGATCAT 
CATGATGTCT ACAAGGTGGA AACCATTGGC GATGCCTACA TGGTGGCTAG TGGTTTGCCT 
AAGAGAAATG GCAATCGGCA TGCAATAGAC ATTGCCAAGA TGGCCTTGGA AATCCTCAGC 
TTCATGGGGA CCTTTGAGCT GGAGCATCTT CCTGGCCTCC CAATATGGAT TCGCATTGGC 
GTTCACTCTG GTCCCTGCGC TGCTGGAGTT GTGGGAATCA AGATGCCTCG TTATTGTCTA 
TTCGGAGATA CAGTCAACAC AGCCTCTAGG ATGGAATCCA CTGGCCTCCC TTTGAGAATT 
CATGTGAGTG GCTCCACCAT AGCCATTCTG AAGAGAACTG AGTGCCAGTT CCTGTATGAA 
GTGAGAGGAG AAACGTACTT AAAGGTAGGG AGTCCACAGA GACAATACAT GGAAAAAGCT 
CTCAGACATG CTTGGGCCAC CTCCTTTGAG CCTTGGCCAG GAAAGTTGAG GAGCATGCTC 
ATCCTTTTCT GTACTCGTCA CAGTGAGCAC CCTCCATCCT CTGCAAGAGA AATGACCACC 
AAAAACTGTG AACTCAGAAT TTTCCTTTAC TCCAAAGAGC CCTTTGGACA TTCTGAACTC 
TGCCACCATT TCCCCTGCCA TATCCAGGTT TTGTATCCAG TTCCTCCTCC AAACACAAGA 
GCTACATACT GTCTGGTCCA GGACTTATTG AATTTAAATC AAAACCCTTA TCCCCCCACT 
TACTTCCCTA CTCTTACTAC ACAACGCTGC TATGATCTGA GGTCACCTCT CTTGCCAGTT 
GCTTTACTAT TCCGAAGCTC TAGAATCACA AGGAATGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007965930.1
CDS	336..3497
Translation	MYSDGLIHNSGDCRSSTCEGLDLLRKISNAKRMGCVLMGPSCTYSTFQMYLDTELSYPMISAGSFGLSCDYKETLTRLMSPARKLTYFLVNFWKTNDLPFKTYSWSTSYVYKNGTESEDCFWYLNALEASVSYFSHELSFKLVLRQDKEFQDILMDHNRKSNVIVMCGGPEFLYKLKGDRAVAEDIVIILVDLFNDQYFEDNVTAPDYMKNVLVLTQSPGNSLLNSSFSRNLSPTKRDFALAYLNGILLFGHVLKIFLENGENITTPKFAHAFRNLTFEGYNGPVTLDDWGDVDSTMVLLYTSVNTKKYKVLLTYDTHVNQTHPVDMSPTFTWKNSKLPNDITDRGPQILMIAVFTLTGAVVLLLLVALLMLRKYKKNYELRQKKWSHIPPENIFPLETNETNHVSLKIDDDKRRDTIQRLRQCKYDKKRVILKDLKHNDGNFTEKQKIELNKLLQIDYYNLTKFYGTVKLDTMIFGVIEYCERGSLREVLNDTISYPDGTFMDWEFKISVLYDIAKGMSYLHSSKTEVHGRLKSTNCVVDSRMVVKITDFGCNSILPPKKDLWTAPEHLRQANVSQKGDVYSYGIIAQEIILRKETFYTSSCRDRNEKIFRVENSNGMKPFRPDLFLETAEEKELEVYLLVKSCWEEDPEKRPDFKKIETTLAKIFGLFHDQKNESYMDTLIRRLQLYSRNLEHLVEERTQLYKAERDRADRLNFMLLPRLVVKSLKEKGFVEPELYEEVTIYFSDIVGFTTICKYSTPMEVVDMLNDIYKSFDHIVDHHDVYKVETIGDAYMVASGLPKRNGNRHAIDIAKMALEILSFMGTFELEHLPGLPIWIRIGVHSGPCAAGVVGIKMPRYCLFGDTVNTASRMESTGLPLRIHVSGSTIAILKRTECQFLYEVRGETYLKVGSPQRQYMEKALRHAWATSFEPWPGKLRSMLILFCTRHSEHPPSSAREMTTKNCELRIFLYSKEPFGHSELCHHFPCHIQVLYPVPPPNTRATYCLVQDLLNLNQNPYPPTYFPTLTTQRCYDLRSPLLPVALLFRSSRITRNE

Target ORF information:

RefSeq Version	XM_007967739.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007967739.1

ORF Insert Sequence:

ATGTATTCGG ATGGTCTGAT TCATAACTCC GGCGACTGCC GGAGCAGCAC CTGTGAAGGC 
CTTGACCTAC TCAGGAAAAT TTCAAATGCA AAACGGATGG GCTGTGTCCT CATGGGGCCC 
TCATGTACAT ACTCCACCTT CCAGATGTAC CTTGACACGG AATTGAGCTA CCCCATGATC 
TCAGCTGGAA GTTTTGGATT GTCATGTGAC TATAAAGAAA CCTTAACCCG GCTGATGTCT 
CCAGCTAGAA AGTTGACATA CTTCTTGGTT AACTTTTGGA AAACCAATGA TCTACCCTTC 
AAAACTTATT CCTGGAGCAC TTCGTATGTT TACAAGAATG GTACGGAGTC CGAGGACTGT 
TTCTGGTACC TTAACGCTCT GGAGGCCAGT GTTTCCTATT TCTCCCACGA ACTCAGTTTT 
AAGTTGGTGT TAAGACAAGA TAAGGAGTTT CAGGATATCT TAATGGACCA CAACAGGAAA 
AGCAATGTGA TTGTTATGTG TGGTGGTCCA GAGTTCCTCT ACAAGTTGAA GGGTGACCGA 
GCAGTGGCTG AAGACATTGT CATTATTCTA GTGGATCTCT TCAATGACCA GTACTTTGAG 
GACAATGTCA CAGCCCCTGA CTATATGAAA AATGTCCTTG TTCTGACGCA GTCTCCTGGG 
AATTCCCTCC TAAATAGCTC TTTCTCCAGG AATCTATCCC CAACAAAACG AGACTTTGCT 
CTTGCCTATT TGAATGGAAT CCTGCTCTTT GGACATGTGC TGAAGATATT TCTTGAAAAT 
GGAGAAAATA TTACCACCCC CAAATTTGCT CATGCTTTCA GGAATCTCAC TTTTGAAGGG 
TATAATGGTC CAGTGACCTT GGATGACTGG GGAGATGTGG ACAGTACCAT GGTGCTTCTG 
TATACGTCTG TGAACACCAA GAAATACAAG GTTCTTTTGA CCTATGATAC CCACGTAAAT 
CAGACCCACC CTGTGGATAT GAGCCCCACA TTCACTTGGA AGAACTCTAA ACTTCCTAAT 
GATATTACAG ACCGGGGCCC TCAGATCCTG ATGATTGCAG TCTTCACCCT CACCGGAGCT 
GTGGTGCTGC TCCTGCTTGT CGCTCTCCTG ATGCTCAGAA AATACAAAAA AAATTATGAA 
CTTCGTCAGA AAAAATGGTC CCACATTCCT CCTGAAAATA TCTTTCCTCT GGAGACCAAT 
GAGACCAATC ACGTTAGCCT GAAGATCGAT GATGACAAAA GACGAGATAC AATCCAGAGA 
CTACGACAGT GCAAATACGA CAAAAAGCGA GTGATTCTCA AAGATCTCAA GCACAATGAT 
GGTAATTTCA CTGAAAAACA GAAGATAGAA TTGAACAAGT TGCTTCAGAT TGACTATTAC 
AACCTGACCA AGTTCTATGG CACAGTGAAA CTTGATACCA TGATCTTCGG GGTGATAGAA 
TACTGTGAGA GAGGATCCCT CCGGGAAGTT TTAAATGACA CAATTTCCTA CCCTGATGGC 
ACATTCATGG ATTGGGAGTT TAAGATCTCT GTCTTGTATG ACATTGCTAA GGGAATGTCA 
TATCTGCACT CCAGTAAGAC AGAAGTCCAT GGTCGTCTGA AATCTACCAA CTGCGTAGTG 
GACAGTAGAA TGGTGGTGAA GATCACTGAT TTTGGCTGCA ATTCCATTTT ACCTCCAAAA 
AAAGACCTGT GGACAGCTCC AGAGCACCTC CGCCAAGCCA ACGTCTCTCA GAAAGGAGAT 
GTGTACAGCT ATGGGATCAT CGCACAGGAG ATCATCCTGC GGAAAGAAAC CTTCTACACT 
TCTAGCTGTC GAGACCGGAA CGAGAAGATT TTCAGAGTGG AAAATTCCAA TGGAATGAAA 
CCCTTCCGCC CAGATTTATT CTTGGAAACG GCAGAGGAAA AAGAGCTAGA AGTGTACCTA 
CTTGTAAAAA GCTGTTGGGA AGAAGATCCA GAAAAGAGAC CAGATTTCAA AAAAATTGAG 
ACTACACTTG CCAAGATATT TGGACTTTTT CATGACCAAA AAAATGAAAG CTATATGGAT 
ACCTTGATCC GACGTCTACA GCTATATTCT CGAAACCTGG AACATCTGGT AGAGGAAAGG 
ACACAGCTAT ACAAGGCAGA GAGGGACAGG GCTGACAGAC TTAACTTTAT GTTGCTTCCA 
AGGCTAGTGG TAAAGTCTCT GAAGGAGAAA GGCTTTGTAG AACCGGAACT ATATGAGGAA 
GTTACAATCT ACTTCAGTGA CATTGTAGGT TTCACTACTA TCTGCAAATA CAGCACCCCC 
ATGGAAGTGG TGGACATGCT TAATGACATC TATAAGAGTT TTGACCACAT TGTTGATCAT 
CATGATGTCT ACAAGGTGGA AACCATTGGC GATGCCTACA TGGTGGCTAG TGGTTTGCCT 
AAGAGAAATG GCAATCGGCA TGCAATAGAC ATTGCCAAGA TGGCCTTGGA AATCCTCAGC 
TTCATGGGGA CCTTTGAGCT GGAGCATCTT CCTGGCCTCC CAATATGGAT TCGCATTGGC 
GTTCACTCTG GTCCCTGCGC TGCTGGAGTT GTGGGAATCA AGATGCCTCG TTATTGTCTA 
TTCGGAGATA CAGTCAACAC AGCCTCTAGG ATGGAATCCA CTGGCCTCCC TTTGAGAATT 
CATGTGAGTG GCTCCACCAT AGCCATTCTG AAGAGAACTG AGTGCCAGTT CCTGTATGAA 
GTGAGAGGAG AAACGTACTT AAAGGTAGGG AGTCCACAGA GACAATACAT GGAAAAAGCT 
CTCAGACATG CTTGGGCCAC CTCCTTTGAG CCTTGGCCAG GAAAGTTGAG GAGCATGCTC 
ATCCTTTTCT GTACTCGTCA CAGTGAGCAC CCTCCATCCT CTGCAAGAGA AATGACCACC 
AAAAACTGTG AACTCAGAAT TTTCCTTTAC TCCAAAGAGC CCTTTGGACA TTCTGAACTC 
TGCCACCATT TCCCCTGCCA TATCCAGGTT TTGTATCCAG TTCCTCCTCC AAACACAAGA 
GCTACATACT GTCTGGTCCA GGACTTATTG AATTTAAATC AAAACCCTTA TCCCCCCACT 
TACTTCCCTA CTCTTACTAC ACAACGCTGC TATGATCTGA GGTCACCTCT CTTGCCAGTT 
GCTTTACTAT TCCGAAGCTC TAGAATCACA AGGAATGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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GUCY2C cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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