HECW2 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW2
Entrez Gene ID	103217568
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Chlorocebus sabaeus(green monkey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007965718.1	XP_007963909.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_007965715.1	XP_007963906.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_007965717.1	XP_007963908.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_007965716.1	XP_007963907.1	E3 ubiquitin-protein ligase HECW2 isoform X1

Pathways from BioSystems
Gene Ontology

The following HECW2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCh94921	XM_007965718.1 Latest version!	Chlorocebus sabaeus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCh94920	XM_007965716.1 Latest version!	Chlorocebus sabaeus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCh94920	XM_007965717.1 Latest version!	Chlorocebus sabaeus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCh94920	XM_007965715.1 Latest version!	Chlorocebus sabaeus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW2 ( XM_007965718.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007963909.1 Chlorocebus sabaeus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCh94921
Clone ID Related Accession (Same CDS sequence)	XM_007965718.1
Accession Version	XM_007965718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735252.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGG AGATTAAAAT CTGTTTTAAA TATTACCACG GCATTAGTGG GGCCCTGCGA 
GCCACGACCC CCTGCATCAC CGTGAAGAAC CCGGCTGTAA TGATGGGGGC AGAAGGCATG 
GAGGGAGGTG CTTCAGGAAA CCTGCATTCT CGAAAACTCG TTAGCTTTAC ATTGTCAGAT 
CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCAATTCAGC CAGGAAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGA 
CGGTCTACTA TCATCAGTAA CACCACCAAT CCAATTTGGC ACCGAGAGAA ATATTCCTTT 
TTTGCACTTC TTACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGCCGT 
CCCATCATCA AGCGTTTTCT GGGGAAACTA ACCATTCCAG TCCAGAGGCT GCTGGAGCGA 
CAGGCTATCG GTGATCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTTCC AGCTGACCAC 
GTGAGTGGGT ACCTCCAGTT TAAAGTGGAG GTTACATCTT CTGTTCATGA AGATGCCTCT 
CCAGAAGCTG TTGGCACAAT ACTTGGAGTC AATTCTGTGA ATGGAGACTT AGGTAGCCCT 
TCCGATGACG AGGACATGCC AGGGAGCCAT CACGACAGCC AGTTGTGCTC TAATGGGCCA 
GTTTCTGAGG ACAGCGCTGC CGATGGGGCG CCCAAACATT CATTTAGGAC TAGCTCCACG 
CTGGAAATAG ACACCGAGGA ATTAACTTCC ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATCGAAC ACAACGGCCA CTCCAGGCCA 
GGGACAGCGA CCTGCTCTGA GAGGTCCATG GGCGCCTCTC CGAAACTGAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCCATGCTT CATATTGACT CAGATGAAGA AGATCACGAG 
TTCCAGCAAG ACCTGGGTTA CCCATCTTCC TTAGAGGAGG AGGGAGGCCT GATCATGTTT 
AGCAGGGCAT CCAGAGCTGA TGATGGAAGC CTGACATCTC AGACAAAACT GGAGGACAAC 
CCTGTTGAGA ATGAGGAAGC CTCCACACAC GAAGCCGCTT CCTTGGAGGA TAAGCCAGAA 
AATCTTCCAG AGCTTGCAGA AAGCTCCTTA CCTGCAGGCC GAGCCCCAGA GGAAGGTGAA 
GGCGGCCCAG AGTCTCAACC CAGTGCTGAC CAGGCCAGTG CTGAACTGTG TGGCTCTCAA 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGGACTT CTGATGCATC AGGAGGAAGC 
CGAAGAGCTG TCAGTGAGAC CGAGTCTCTC GATCAGGGCT CCGAGCCTTC CCAGGTGTCC 
TCTGAAACAG AACCCAGTGA TCCTGCCAGG ACAGAGAGTG TGAGCGAAGC CAGCACCAGG 
CCCGAGGGAG AGAGTGACCT GGAATGCGCT GACAGCTCTT GCAATGAGAG TGTGACCACG 
CAGCTGTCCT CTGTGGAGAC GCGGTGCTCC TCTCTTGAGA GTGCACGGTT TCCTGAAACC 
CCAGCCTTCT CTTCTCAGGA GGAGGAAGAC GGAGCCTGTG CAGCAGAGCC CACCAGCAGC 
GGCCCTGCCG AAGGGTCGCA GGATTCCGTG TGCACTGCTG GTTCTTTACC TGTGGTACAG 
GTGCCCAGTG GGGAGGAGGA AGGGCCAGGG GCAGAATCGG CCACTGTACC TGACCAGGAG 
GAGCTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCAGC TGCTGCCGAG 
AGCCCTCCGC AAGAAGAGGG CAGTGCTGGG GAGGCCCAAG GCACCTGTGA AGGGGCAACT 
GCCCAGGAGG AGGGCGCTAC TGGAGGTTCT CAAGCCAACG GCCACCAGCC ACTGCGATCA 
CTACCTTCAG TGCGCCAGGA TGTTAGCCGG TACCAGAGGG TGGACGAGGC TCTTCCACCA 
AACTGGGAGG CACGCATTGA CAGCCACGGC AGGATCTTCT ACGTGGATCA CGTAAACAGA 
ACCACAACGT GGCAGCGACC GACAGCTCCC CCAGCCCCGC AGGTGCTGCA GAGATCTAAC 
TCCATACAGC AAATGGAGCA GCTGAACCGG CGATATCAGA GCATCCGCAG AACCATGACC 
AATGAGAGGC CTGAGGAAAA TACCAATGCT ATCGATGGGG CAGGGGAGGA AACTGACTTT 
CACCAAGCCA GTGCAGATTT CCGACGGGAG AACATCCTGC CTCACTCTAC CTCCAGATCC 
AGGCTCACGT TGCTGTTACA GTCTCCCCCC GTGAAGTTCC TCATCAGCCC AGAGTTCTTC 
ACCGTGCTGC ATTCTAACCC TAGTGCCTAC CGCATGTTTA CAAACAACAC GTGTTTGAAG 
CACATGATCA CCAAAGTCCG GCGGGACACC CACCACTTTG AACGCTACCA GCATAACCGG 
GACCTTGTGG GATTCCTCAA CATGTTTGCC AACAAACAGC TAGAGCTGCC GCGGGGATGG 
GAAATGAAAC ATGATCACCA GGGCAAGGCG TTCTTTGTGG ACCACAACTC CCGTACCACC 
ACTTTCATTG ATCCCCGGCT CCCACTTCAG AGCAGTAGAC CTACAAGTGC ACTGGTTCAT 
CGGCAACACC TGACAAGGCA ACGCAGCCAC AGTGCGGGTG AGGTAGGAGA AGATTCTCGA 
CATGCAGGAC CACCAGTTCT TCCCAGGCCA TCCAGTACAT TCAATACAGT CAGTAGGCCA 
CAGTACCAGG ACACGGTTCC AGTGGCTTAC AATGACAAGA TTGTTGCATT TCTACGTCAA 
CCCAATATCT TTGAAATTCT ACAGGAGCGT CAACCTGATC TTACCAGGAA TCACTCACTC 
AGGGAGAAGA TCCAATTTAT CCGAACTGAA GGGACTCCAG GATTGGTGCG CCTTTCAAGC 
GATGCAGACC TCGTTATGTT GCTGAGCTTA TTTGAAGAAG AGATAATGTC ATATGTGCCT 
CCACATGCCT TACTCTACCC CAGCTACTGT CAGTCCCCAC GTGGCTCTCC CGTGTCATCT 
CCTCAGAACT CACCAGGTAC CCAGCGTGCC AATGCCCGGG CTCCAGCCCC TTACAAGCGG 
GATTTCGAAG CCAAACTGAG GAACTTTTAC AGGAAGTTAG AGACTAAAGG ATATGGACAA 
GGCCCAGGGA AGTTAAAGTT AATTATCCGA AGAGATCACT TACTAGAAGA TGCTTTTAAT 
CAGATTATGG GCTACTCCAG AAAAGACCTG CAGAGAAATA AGCTATATGT CACCTTCGTC 
GGGGAGGAAG GGCTGGATTA CAGTGGGCCT TCCAGAGAGT TTTTCTTCCT GGTATCCAGA 
GAACTCTTTA ACCCATATTA TGGCTTATTT GAATATTCAG CCAATGACAC ATACACAGTA 
CAAATAAGTC CTATGTCTGC TTTTGTAGAC AATCACCATG AATGGTTCCG ATTCAGTGGT 
AGGATCCTTG GTCTTGCACT AATACACCAG TATTTGTTGG ATGCCTTCTT CACACGGCCC 
TTTTATAAGG CTCTTCTCAG AATTCTATGT GACCTGAGTG ATCTAGAATA CCTTGATGAA 
GAGTTCCATC AGAGCCTGCA GTGGATGAAA GACAATGATA TCCATGACAT CCTAGACCTC 
ACGTTCACTG TGAACGAAGA AGTATTTGGG CAGATAACTG AACGAGAATT AAAGCCTGGG 
GGTGCCAATA TCCCAGTTAC AGAGAAGAAC AAGAAGGAGT ACATCGAGAG GATGGTGAAG 
TGGAGGATTG AGAGGGGTGT TGTACAACAA ACAGAGAGTT TAGTGCGTGG CTTCTATGAG 
GTGGTGGATG CCAGGCTGGT ATCTGTATTT GATGCAAGAG AACTGGAATT GGTCATCGCA 
GGCACAGCTG AAATAGACCT AAGTGATTGG AGAAACAACA CAGAATATAG AGGAGGATAC 
CATGACAATC ATATTGTAAT TCGGTGGTTC TGGGCTGCTG TGGAGAGATT CAACAATGAA 
CAACGACTAA GGTTGTTACA GTTTGTTACG GGCACATCCA GCATTCCCTA CGAAGGATTT 
GCTTCTCTCC GAGGGAGTAA CGGCCCAAGA AGATTCTGTG TGGAGAAATG GGGGAAAATC 
ACTGCTCTTC CCAGAGCGCA CACATGTTTT AACCGTCTGG ATCTGCCCCC CTACCCATCC 
TTTTCCATGC TTTATGAGAA ACTGTTGACA GCAGTTGAAG AAACCAGTAC TTTTGGACTT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007963909.1
CDS	1860..6185
Translation	MEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQLCSNGPVSEDSAADGAPKHSFRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTHEAASLEDKPENLPELAESSLPAGRAPEEGEGGPESQPSADQASAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSVCTAGSLPVVQVPSGEEEGPGAESATVPDQEELGEVWQRRGSLEGAAAAAESPPQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEETDFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDTVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLYPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_007965718.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007965718.1

ORF Insert Sequence:

ATGGAACCGG AGATTAAAAT CTGTTTTAAA TATTACCACG GCATTAGTGG GGCCCTGCGA 
GCCACGACCC CCTGCATCAC CGTGAAGAAC CCGGCTGTAA TGATGGGGGC AGAAGGCATG 
GAGGGAGGTG CTTCAGGAAA CCTGCATTCT CGAAAACTCG TTAGCTTTAC ATTGTCAGAT 
CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCAATTCAGC CAGGAAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGA 
CGGTCTACTA TCATCAGTAA CACCACCAAT CCAATTTGGC ACCGAGAGAA ATATTCCTTT 
TTTGCACTTC TTACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGCCGT 
CCCATCATCA AGCGTTTTCT GGGGAAACTA ACCATTCCAG TCCAGAGGCT GCTGGAGCGA 
CAGGCTATCG GTGATCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTTCC AGCTGACCAC 
GTGAGTGGGT ACCTCCAGTT TAAAGTGGAG GTTACATCTT CTGTTCATGA AGATGCCTCT 
CCAGAAGCTG TTGGCACAAT ACTTGGAGTC AATTCTGTGA ATGGAGACTT AGGTAGCCCT 
TCCGATGACG AGGACATGCC AGGGAGCCAT CACGACAGCC AGTTGTGCTC TAATGGGCCA 
GTTTCTGAGG ACAGCGCTGC CGATGGGGCG CCCAAACATT CATTTAGGAC TAGCTCCACG 
CTGGAAATAG ACACCGAGGA ATTAACTTCC ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATCGAAC ACAACGGCCA CTCCAGGCCA 
GGGACAGCGA CCTGCTCTGA GAGGTCCATG GGCGCCTCTC CGAAACTGAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCCATGCTT CATATTGACT CAGATGAAGA AGATCACGAG 
TTCCAGCAAG ACCTGGGTTA CCCATCTTCC TTAGAGGAGG AGGGAGGCCT GATCATGTTT 
AGCAGGGCAT CCAGAGCTGA TGATGGAAGC CTGACATCTC AGACAAAACT GGAGGACAAC 
CCTGTTGAGA ATGAGGAAGC CTCCACACAC GAAGCCGCTT CCTTGGAGGA TAAGCCAGAA 
AATCTTCCAG AGCTTGCAGA AAGCTCCTTA CCTGCAGGCC GAGCCCCAGA GGAAGGTGAA 
GGCGGCCCAG AGTCTCAACC CAGTGCTGAC CAGGCCAGTG CTGAACTGTG TGGCTCTCAA 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGGACTT CTGATGCATC AGGAGGAAGC 
CGAAGAGCTG TCAGTGAGAC CGAGTCTCTC GATCAGGGCT CCGAGCCTTC CCAGGTGTCC 
TCTGAAACAG AACCCAGTGA TCCTGCCAGG ACAGAGAGTG TGAGCGAAGC CAGCACCAGG 
CCCGAGGGAG AGAGTGACCT GGAATGCGCT GACAGCTCTT GCAATGAGAG TGTGACCACG 
CAGCTGTCCT CTGTGGAGAC GCGGTGCTCC TCTCTTGAGA GTGCACGGTT TCCTGAAACC 
CCAGCCTTCT CTTCTCAGGA GGAGGAAGAC GGAGCCTGTG CAGCAGAGCC CACCAGCAGC 
GGCCCTGCCG AAGGGTCGCA GGATTCCGTG TGCACTGCTG GTTCTTTACC TGTGGTACAG 
GTGCCCAGTG GGGAGGAGGA AGGGCCAGGG GCAGAATCGG CCACTGTACC TGACCAGGAG 
GAGCTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCAGC TGCTGCCGAG 
AGCCCTCCGC AAGAAGAGGG CAGTGCTGGG GAGGCCCAAG GCACCTGTGA AGGGGCAACT 
GCCCAGGAGG AGGGCGCTAC TGGAGGTTCT CAAGCCAACG GCCACCAGCC ACTGCGATCA 
CTACCTTCAG TGCGCCAGGA TGTTAGCCGG TACCAGAGGG TGGACGAGGC TCTTCCACCA 
AACTGGGAGG CACGCATTGA CAGCCACGGC AGGATCTTCT ACGTGGATCA CGTAAACAGA 
ACCACAACGT GGCAGCGACC GACAGCTCCC CCAGCCCCGC AGGTGCTGCA GAGATCTAAC 
TCCATACAGC AAATGGAGCA GCTGAACCGG CGATATCAGA GCATCCGCAG AACCATGACC 
AATGAGAGGC CTGAGGAAAA TACCAATGCT ATCGATGGGG CAGGGGAGGA AACTGACTTT 
CACCAAGCCA GTGCAGATTT CCGACGGGAG AACATCCTGC CTCACTCTAC CTCCAGATCC 
AGGCTCACGT TGCTGTTACA GTCTCCCCCC GTGAAGTTCC TCATCAGCCC AGAGTTCTTC 
ACCGTGCTGC ATTCTAACCC TAGTGCCTAC CGCATGTTTA CAAACAACAC GTGTTTGAAG 
CACATGATCA CCAAAGTCCG GCGGGACACC CACCACTTTG AACGCTACCA GCATAACCGG 
GACCTTGTGG GATTCCTCAA CATGTTTGCC AACAAACAGC TAGAGCTGCC GCGGGGATGG 
GAAATGAAAC ATGATCACCA GGGCAAGGCG TTCTTTGTGG ACCACAACTC CCGTACCACC 
ACTTTCATTG ATCCCCGGCT CCCACTTCAG AGCAGTAGAC CTACAAGTGC ACTGGTTCAT 
CGGCAACACC TGACAAGGCA ACGCAGCCAC AGTGCGGGTG AGGTAGGAGA AGATTCTCGA 
CATGCAGGAC CACCAGTTCT TCCCAGGCCA TCCAGTACAT TCAATACAGT CAGTAGGCCA 
CAGTACCAGG ACACGGTTCC AGTGGCTTAC AATGACAAGA TTGTTGCATT TCTACGTCAA 
CCCAATATCT TTGAAATTCT ACAGGAGCGT CAACCTGATC TTACCAGGAA TCACTCACTC 
AGGGAGAAGA TCCAATTTAT CCGAACTGAA GGGACTCCAG GATTGGTGCG CCTTTCAAGC 
GATGCAGACC TCGTTATGTT GCTGAGCTTA TTTGAAGAAG AGATAATGTC ATATGTGCCT 
CCACATGCCT TACTCTACCC CAGCTACTGT CAGTCCCCAC GTGGCTCTCC CGTGTCATCT 
CCTCAGAACT CACCAGGTAC CCAGCGTGCC AATGCCCGGG CTCCAGCCCC TTACAAGCGG 
GATTTCGAAG CCAAACTGAG GAACTTTTAC AGGAAGTTAG AGACTAAAGG ATATGGACAA 
GGCCCAGGGA AGTTAAAGTT AATTATCCGA AGAGATCACT TACTAGAAGA TGCTTTTAAT 
CAGATTATGG GCTACTCCAG AAAAGACCTG CAGAGAAATA AGCTATATGT CACCTTCGTC 
GGGGAGGAAG GGCTGGATTA CAGTGGGCCT TCCAGAGAGT TTTTCTTCCT GGTATCCAGA 
GAACTCTTTA ACCCATATTA TGGCTTATTT GAATATTCAG CCAATGACAC ATACACAGTA 
CAAATAAGTC CTATGTCTGC TTTTGTAGAC AATCACCATG AATGGTTCCG ATTCAGTGGT 
AGGATCCTTG GTCTTGCACT AATACACCAG TATTTGTTGG ATGCCTTCTT CACACGGCCC 
TTTTATAAGG CTCTTCTCAG AATTCTATGT GACCTGAGTG ATCTAGAATA CCTTGATGAA 
GAGTTCCATC AGAGCCTGCA GTGGATGAAA GACAATGATA TCCATGACAT CCTAGACCTC 
ACGTTCACTG TGAACGAAGA AGTATTTGGG CAGATAACTG AACGAGAATT AAAGCCTGGG 
GGTGCCAATA TCCCAGTTAC AGAGAAGAAC AAGAAGGAGT ACATCGAGAG GATGGTGAAG 
TGGAGGATTG AGAGGGGTGT TGTACAACAA ACAGAGAGTT TAGTGCGTGG CTTCTATGAG 
GTGGTGGATG CCAGGCTGGT ATCTGTATTT GATGCAAGAG AACTGGAATT GGTCATCGCA 
GGCACAGCTG AAATAGACCT AAGTGATTGG AGAAACAACA CAGAATATAG AGGAGGATAC 
CATGACAATC ATATTGTAAT TCGGTGGTTC TGGGCTGCTG TGGAGAGATT CAACAATGAA 
CAACGACTAA GGTTGTTACA GTTTGTTACG GGCACATCCA GCATTCCCTA CGAAGGATTT 
GCTTCTCTCC GAGGGAGTAA CGGCCCAAGA AGATTCTGTG TGGAGAAATG GGGGAAAATC 
ACTGCTCTTC CCAGAGCGCA CACATGTTTT AACCGTCTGG ATCTGCCCCC CTACCCATCC 
TTTTCCATGC TTTATGAGAA ACTGTTGACA GCAGTTGAAG AAACCAGTAC TTTTGGACTT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_007965715.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007963906.1 Chlorocebus sabaeus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCh94920
Clone ID Related Accession (Same CDS sequence)	XM_007965715.1 , XM_007965717.1 , XM_007965716.1
Accession Version	XM_007965715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735252.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTACTT TTTGTGCGGC GTCGAAATCC CCAGATGCGG 
TACACGCTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATTAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGGGCCCTG CGAGCCACGA CCCCCTGCAT CACCGTGAAG 
AACCCGGCTG TAATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TCGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAGGCTA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT TCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAATTCTG TGAATGGAGA CTTAGGTAGC CCTTCCGATG ACGAGGACAT GCCAGGGAGC 
CATCACGACA GCCAGTTGTG CTCTAATGGG CCAGTTTCTG AGGACAGCGC TGCCGATGGG 
GCGCCCAAAC ATTCATTTAG GACTAGCTCC ACGCTGGAAA TAGACACCGA GGAATTAACT 
TCCACTTCTT CAAGGACCTC ACCTCCCAGA GGACGTCAGG ATTCACTCAA TGATTACTTA 
GATGCTATCG AACACAACGG CCACTCCAGG CCAGGGACAG CGACCTGCTC TGAGAGGTCC 
ATGGGCGCCT CTCCGAAACT GAGGAGTAGT TTTCCCACCG ACACAAGACT CAATGCCATG 
CTTCATATTG ACTCAGATGA AGAAGATCAC GAGTTCCAGC AAGACCTGGG TTACCCATCT 
TCCTTAGAGG AGGAGGGAGG CCTGATCATG TTTAGCAGGG CATCCAGAGC TGATGATGGA 
AGCCTGACAT CTCAGACAAA ACTGGAGGAC AACCCTGTTG AGAATGAGGA AGCCTCCACA 
CACGAAGCCG CTTCCTTGGA GGATAAGCCA GAAAATCTTC CAGAGCTTGC AGAAAGCTCC 
TTACCTGCAG GCCGAGCCCC AGAGGAAGGT GAAGGCGGCC CAGAGTCTCA ACCCAGTGCT 
GACCAGGCCA GTGCTGAACT GTGTGGCTCT CAAGAGGTAG ATCAGCCCAC AAGTGGGGCA 
GACACGGGGA CTTCTGATGC ATCAGGAGGA AGCCGAAGAG CTGTCAGTGA GACCGAGTCT 
CTCGATCAGG GCTCCGAGCC TTCCCAGGTG TCCTCTGAAA CAGAACCCAG TGATCCTGCC 
AGGACAGAGA GTGTGAGCGA AGCCAGCACC AGGCCCGAGG GAGAGAGTGA CCTGGAATGC 
GCTGACAGCT CTTGCAATGA GAGTGTGACC ACGCAGCTGT CCTCTGTGGA GACGCGGTGC 
TCCTCTCTTG AGAGTGCACG GTTTCCTGAA ACCCCAGCCT TCTCTTCTCA GGAGGAGGAA 
GACGGAGCCT GTGCAGCAGA GCCCACCAGC AGCGGCCCTG CCGAAGGGTC GCAGGATTCC 
GTGTGCACTG CTGGTTCTTT ACCTGTGGTA CAGGTGCCCA GTGGGGAGGA GGAAGGGCCA 
GGGGCAGAAT CGGCCACTGT ACCTGACCAG GAGGAGCTGG GGGAGGTCTG GCAGCGGAGG 
GGGAGCCTGG AGGGAGCTGC AGCTGCTGCC GAGAGCCCTC CGCAAGAAGA GGGCAGTGCT 
GGGGAGGCCC AAGGCACCTG TGAAGGGGCA ACTGCCCAGG AGGAGGGCGC TACTGGAGGT 
TCTCAAGCCA ACGGCCACCA GCCACTGCGA TCACTACCTT CAGTGCGCCA GGATGTTAGC 
CGGTACCAGA GGGTGGACGA GGCTCTTCCA CCAAACTGGG AGGCACGCAT TGACAGCCAC 
GGCAGGATCT TCTACGTGGA TCACGTAAAC AGAACCACAA CGTGGCAGCG ACCGACAGCT 
CCCCCAGCCC CGCAGGTGCT GCAGAGATCT AACTCCATAC AGCAAATGGA GCAGCTGAAC 
CGGCGATATC AGAGCATCCG CAGAACCATG ACCAATGAGA GGCCTGAGGA AAATACCAAT 
GCTATCGATG GGGCAGGGGA GGAAACTGAC TTTCACCAAG CCAGTGCAGA TTTCCGACGG 
GAGAACATCC TGCCTCACTC TACCTCCAGA TCCAGGCTCA CGTTGCTGTT ACAGTCTCCC 
CCCGTGAAGT TCCTCATCAG CCCAGAGTTC TTCACCGTGC TGCATTCTAA CCCTAGTGCC 
TACCGCATGT TTACAAACAA CACGTGTTTG AAGCACATGA TCACCAAAGT CCGGCGGGAC 
ACCCACCACT TTGAACGCTA CCAGCATAAC CGGGACCTTG TGGGATTCCT CAACATGTTT 
GCCAACAAAC AGCTAGAGCT GCCGCGGGGA TGGGAAATGA AACATGATCA CCAGGGCAAG 
GCGTTCTTTG TGGACCACAA CTCCCGTACC ACCACTTTCA TTGATCCCCG GCTCCCACTT 
CAGAGCAGTA GACCTACAAG TGCACTGGTT CATCGGCAAC ACCTGACAAG GCAACGCAGC 
CACAGTGCGG GTGAGGTAGG AGAAGATTCT CGACATGCAG GACCACCAGT TCTTCCCAGG 
CCATCCAGTA CATTCAATAC AGTCAGTAGG CCACAGTACC AGGACACGGT TCCAGTGGCT 
TACAATGACA AGATTGTTGC ATTTCTACGT CAACCCAATA TCTTTGAAAT TCTACAGGAG 
CGTCAACCTG ATCTTACCAG GAATCACTCA CTCAGGGAGA AGATCCAATT TATCCGAACT 
GAAGGGACTC CAGGATTGGT GCGCCTTTCA AGCGATGCAG ACCTCGTTAT GTTGCTGAGC 
TTATTTGAAG AAGAGATAAT GTCATATGTG CCTCCACATG CCTTACTCTA CCCCAGCTAC 
TGTCAGTCCC CACGTGGCTC TCCCGTGTCA TCTCCTCAGA ACTCACCAGG TACCCAGCGT 
GCCAATGCCC GGGCTCCAGC CCCTTACAAG CGGGATTTCG AAGCCAAACT GAGGAACTTT 
TACAGGAAGT TAGAGACTAA AGGATATGGA CAAGGCCCAG GGAAGTTAAA GTTAATTATC 
CGAAGAGATC ACTTACTAGA AGATGCTTTT AATCAGATTA TGGGCTACTC CAGAAAAGAC 
CTGCAGAGAA ATAAGCTATA TGTCACCTTC GTCGGGGAGG AAGGGCTGGA TTACAGTGGG 
CCTTCCAGAG AGTTTTTCTT CCTGGTATCC AGAGAACTCT TTAACCCATA TTATGGCTTA 
TTTGAATATT CAGCCAATGA CACATACACA GTACAAATAA GTCCTATGTC TGCTTTTGTA 
GACAATCACC ATGAATGGTT CCGATTCAGT GGTAGGATCC TTGGTCTTGC ACTAATACAC 
CAGTATTTGT TGGATGCCTT CTTCACACGG CCCTTTTATA AGGCTCTTCT CAGAATTCTA 
TGTGACCTGA GTGATCTAGA ATACCTTGAT GAAGAGTTCC ATCAGAGCCT GCAGTGGATG 
AAAGACAATG ATATCCATGA CATCCTAGAC CTCACGTTCA CTGTGAACGA AGAAGTATTT 
GGGCAGATAA CTGAACGAGA ATTAAAGCCT GGGGGTGCCA ATATCCCAGT TACAGAGAAG 
AACAAGAAGG AGTACATCGA GAGGATGGTG AAGTGGAGGA TTGAGAGGGG TGTTGTACAA 
CAAACAGAGA GTTTAGTGCG TGGCTTCTAT GAGGTGGTGG ATGCCAGGCT GGTATCTGTA 
TTTGATGCAA GAGAACTGGA ATTGGTCATC GCAGGCACAG CTGAAATAGA CCTAAGTGAT 
TGGAGAAACA ACACAGAATA TAGAGGAGGA TACCATGACA ATCATATTGT AATTCGGTGG 
TTCTGGGCTG CTGTGGAGAG ATTCAACAAT GAACAACGAC TAAGGTTGTT ACAGTTTGTT 
ACGGGCACAT CCAGCATTCC CTACGAAGGA TTTGCTTCTC TCCGAGGGAG TAACGGCCCA 
AGAAGATTCT GTGTGGAGAA ATGGGGGAAA ATCACTGCTC TTCCCAGAGC GCACACATGT 
TTTAACCGTC TGGATCTGCC CCCCTACCCA TCCTTTTCCA TGCTTTATGA GAAACTGTTG 
ACAGCAGTTG AAGAAACCAG TACTTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007963906.1
CDS	108..4826
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQLCSNGPVSEDSAADGAPKHSFRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTHEAASLEDKPENLPELAESSLPAGRAPEEGEGGPESQPSADQASAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSVCTAGSLPVVQVPSGEEEGPGAESATVPDQEELGEVWQRRGSLEGAAAAAESPPQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEETDFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDTVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLYPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_007965715.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007965715.1

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTACTT TTTGTGCGGC GTCGAAATCC CCAGATGCGG 
TACACGCTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATTAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGGGCCCTG CGAGCCACGA CCCCCTGCAT CACCGTGAAG 
AACCCGGCTG TAATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TCGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAGGCTA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT TCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAATTCTG TGAATGGAGA CTTAGGTAGC CCTTCCGATG ACGAGGACAT GCCAGGGAGC 
CATCACGACA GCCAGTTGTG CTCTAATGGG CCAGTTTCTG AGGACAGCGC TGCCGATGGG 
GCGCCCAAAC ATTCATTTAG GACTAGCTCC ACGCTGGAAA TAGACACCGA GGAATTAACT 
TCCACTTCTT CAAGGACCTC ACCTCCCAGA GGACGTCAGG ATTCACTCAA TGATTACTTA 
GATGCTATCG AACACAACGG CCACTCCAGG CCAGGGACAG CGACCTGCTC TGAGAGGTCC 
ATGGGCGCCT CTCCGAAACT GAGGAGTAGT TTTCCCACCG ACACAAGACT CAATGCCATG 
CTTCATATTG ACTCAGATGA AGAAGATCAC GAGTTCCAGC AAGACCTGGG TTACCCATCT 
TCCTTAGAGG AGGAGGGAGG CCTGATCATG TTTAGCAGGG CATCCAGAGC TGATGATGGA 
AGCCTGACAT CTCAGACAAA ACTGGAGGAC AACCCTGTTG AGAATGAGGA AGCCTCCACA 
CACGAAGCCG CTTCCTTGGA GGATAAGCCA GAAAATCTTC CAGAGCTTGC AGAAAGCTCC 
TTACCTGCAG GCCGAGCCCC AGAGGAAGGT GAAGGCGGCC CAGAGTCTCA ACCCAGTGCT 
GACCAGGCCA GTGCTGAACT GTGTGGCTCT CAAGAGGTAG ATCAGCCCAC AAGTGGGGCA 
GACACGGGGA CTTCTGATGC ATCAGGAGGA AGCCGAAGAG CTGTCAGTGA GACCGAGTCT 
CTCGATCAGG GCTCCGAGCC TTCCCAGGTG TCCTCTGAAA CAGAACCCAG TGATCCTGCC 
AGGACAGAGA GTGTGAGCGA AGCCAGCACC AGGCCCGAGG GAGAGAGTGA CCTGGAATGC 
GCTGACAGCT CTTGCAATGA GAGTGTGACC ACGCAGCTGT CCTCTGTGGA GACGCGGTGC 
TCCTCTCTTG AGAGTGCACG GTTTCCTGAA ACCCCAGCCT TCTCTTCTCA GGAGGAGGAA 
GACGGAGCCT GTGCAGCAGA GCCCACCAGC AGCGGCCCTG CCGAAGGGTC GCAGGATTCC 
GTGTGCACTG CTGGTTCTTT ACCTGTGGTA CAGGTGCCCA GTGGGGAGGA GGAAGGGCCA 
GGGGCAGAAT CGGCCACTGT ACCTGACCAG GAGGAGCTGG GGGAGGTCTG GCAGCGGAGG 
GGGAGCCTGG AGGGAGCTGC AGCTGCTGCC GAGAGCCCTC CGCAAGAAGA GGGCAGTGCT 
GGGGAGGCCC AAGGCACCTG TGAAGGGGCA ACTGCCCAGG AGGAGGGCGC TACTGGAGGT 
TCTCAAGCCA ACGGCCACCA GCCACTGCGA TCACTACCTT CAGTGCGCCA GGATGTTAGC 
CGGTACCAGA GGGTGGACGA GGCTCTTCCA CCAAACTGGG AGGCACGCAT TGACAGCCAC 
GGCAGGATCT TCTACGTGGA TCACGTAAAC AGAACCACAA CGTGGCAGCG ACCGACAGCT 
CCCCCAGCCC CGCAGGTGCT GCAGAGATCT AACTCCATAC AGCAAATGGA GCAGCTGAAC 
CGGCGATATC AGAGCATCCG CAGAACCATG ACCAATGAGA GGCCTGAGGA AAATACCAAT 
GCTATCGATG GGGCAGGGGA GGAAACTGAC TTTCACCAAG CCAGTGCAGA TTTCCGACGG 
GAGAACATCC TGCCTCACTC TACCTCCAGA TCCAGGCTCA CGTTGCTGTT ACAGTCTCCC 
CCCGTGAAGT TCCTCATCAG CCCAGAGTTC TTCACCGTGC TGCATTCTAA CCCTAGTGCC 
TACCGCATGT TTACAAACAA CACGTGTTTG AAGCACATGA TCACCAAAGT CCGGCGGGAC 
ACCCACCACT TTGAACGCTA CCAGCATAAC CGGGACCTTG TGGGATTCCT CAACATGTTT 
GCCAACAAAC AGCTAGAGCT GCCGCGGGGA TGGGAAATGA AACATGATCA CCAGGGCAAG 
GCGTTCTTTG TGGACCACAA CTCCCGTACC ACCACTTTCA TTGATCCCCG GCTCCCACTT 
CAGAGCAGTA GACCTACAAG TGCACTGGTT CATCGGCAAC ACCTGACAAG GCAACGCAGC 
CACAGTGCGG GTGAGGTAGG AGAAGATTCT CGACATGCAG GACCACCAGT TCTTCCCAGG 
CCATCCAGTA CATTCAATAC AGTCAGTAGG CCACAGTACC AGGACACGGT TCCAGTGGCT 
TACAATGACA AGATTGTTGC ATTTCTACGT CAACCCAATA TCTTTGAAAT TCTACAGGAG 
CGTCAACCTG ATCTTACCAG GAATCACTCA CTCAGGGAGA AGATCCAATT TATCCGAACT 
GAAGGGACTC CAGGATTGGT GCGCCTTTCA AGCGATGCAG ACCTCGTTAT GTTGCTGAGC 
TTATTTGAAG AAGAGATAAT GTCATATGTG CCTCCACATG CCTTACTCTA CCCCAGCTAC 
TGTCAGTCCC CACGTGGCTC TCCCGTGTCA TCTCCTCAGA ACTCACCAGG TACCCAGCGT 
GCCAATGCCC GGGCTCCAGC CCCTTACAAG CGGGATTTCG AAGCCAAACT GAGGAACTTT 
TACAGGAAGT TAGAGACTAA AGGATATGGA CAAGGCCCAG GGAAGTTAAA GTTAATTATC 
CGAAGAGATC ACTTACTAGA AGATGCTTTT AATCAGATTA TGGGCTACTC CAGAAAAGAC 
CTGCAGAGAA ATAAGCTATA TGTCACCTTC GTCGGGGAGG AAGGGCTGGA TTACAGTGGG 
CCTTCCAGAG AGTTTTTCTT CCTGGTATCC AGAGAACTCT TTAACCCATA TTATGGCTTA 
TTTGAATATT CAGCCAATGA CACATACACA GTACAAATAA GTCCTATGTC TGCTTTTGTA 
GACAATCACC ATGAATGGTT CCGATTCAGT GGTAGGATCC TTGGTCTTGC ACTAATACAC 
CAGTATTTGT TGGATGCCTT CTTCACACGG CCCTTTTATA AGGCTCTTCT CAGAATTCTA 
TGTGACCTGA GTGATCTAGA ATACCTTGAT GAAGAGTTCC ATCAGAGCCT GCAGTGGATG 
AAAGACAATG ATATCCATGA CATCCTAGAC CTCACGTTCA CTGTGAACGA AGAAGTATTT 
GGGCAGATAA CTGAACGAGA ATTAAAGCCT GGGGGTGCCA ATATCCCAGT TACAGAGAAG 
AACAAGAAGG AGTACATCGA GAGGATGGTG AAGTGGAGGA TTGAGAGGGG TGTTGTACAA 
CAAACAGAGA GTTTAGTGCG TGGCTTCTAT GAGGTGGTGG ATGCCAGGCT GGTATCTGTA 
TTTGATGCAA GAGAACTGGA ATTGGTCATC GCAGGCACAG CTGAAATAGA CCTAAGTGAT 
TGGAGAAACA ACACAGAATA TAGAGGAGGA TACCATGACA ATCATATTGT AATTCGGTGG 
TTCTGGGCTG CTGTGGAGAG ATTCAACAAT GAACAACGAC TAAGGTTGTT ACAGTTTGTT 
ACGGGCACAT CCAGCATTCC CTACGAAGGA TTTGCTTCTC TCCGAGGGAG TAACGGCCCA 
AGAAGATTCT GTGTGGAGAA ATGGGGGAAA ATCACTGCTC TTCCCAGAGC GCACACATGT 
TTTAACCGTC TGGATCTGCC CCCCTACCCA TCCTTTTCCA TGCTTTATGA GAAACTGTTG 
ACAGCAGTTG AAGAAACCAG TACTTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_007965717.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007963908.1 Chlorocebus sabaeus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

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CloneID	OCh94920
Clone ID Related Accession (Same CDS sequence)	XM_007965715.1 , XM_007965717.1 , XM_007965716.1
Accession Version	XM_007965717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735252.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTACTT TTTGTGCGGC GTCGAAATCC CCAGATGCGG 
TACACGCTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATTAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGGGCCCTG CGAGCCACGA CCCCCTGCAT CACCGTGAAG 
AACCCGGCTG TAATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TCGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAGGCTA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT TCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAATTCTG TGAATGGAGA CTTAGGTAGC CCTTCCGATG ACGAGGACAT GCCAGGGAGC 
CATCACGACA GCCAGTTGTG CTCTAATGGG CCAGTTTCTG AGGACAGCGC TGCCGATGGG 
GCGCCCAAAC ATTCATTTAG GACTAGCTCC ACGCTGGAAA TAGACACCGA GGAATTAACT 
TCCACTTCTT CAAGGACCTC ACCTCCCAGA GGACGTCAGG ATTCACTCAA TGATTACTTA 
GATGCTATCG AACACAACGG CCACTCCAGG CCAGGGACAG CGACCTGCTC TGAGAGGTCC 
ATGGGCGCCT CTCCGAAACT GAGGAGTAGT TTTCCCACCG ACACAAGACT CAATGCCATG 
CTTCATATTG ACTCAGATGA AGAAGATCAC GAGTTCCAGC AAGACCTGGG TTACCCATCT 
TCCTTAGAGG AGGAGGGAGG CCTGATCATG TTTAGCAGGG CATCCAGAGC TGATGATGGA 
AGCCTGACAT CTCAGACAAA ACTGGAGGAC AACCCTGTTG AGAATGAGGA AGCCTCCACA 
CACGAAGCCG CTTCCTTGGA GGATAAGCCA GAAAATCTTC CAGAGCTTGC AGAAAGCTCC 
TTACCTGCAG GCCGAGCCCC AGAGGAAGGT GAAGGCGGCC CAGAGTCTCA ACCCAGTGCT 
GACCAGGCCA GTGCTGAACT GTGTGGCTCT CAAGAGGTAG ATCAGCCCAC AAGTGGGGCA 
GACACGGGGA CTTCTGATGC ATCAGGAGGA AGCCGAAGAG CTGTCAGTGA GACCGAGTCT 
CTCGATCAGG GCTCCGAGCC TTCCCAGGTG TCCTCTGAAA CAGAACCCAG TGATCCTGCC 
AGGACAGAGA GTGTGAGCGA AGCCAGCACC AGGCCCGAGG GAGAGAGTGA CCTGGAATGC 
GCTGACAGCT CTTGCAATGA GAGTGTGACC ACGCAGCTGT CCTCTGTGGA GACGCGGTGC 
TCCTCTCTTG AGAGTGCACG GTTTCCTGAA ACCCCAGCCT TCTCTTCTCA GGAGGAGGAA 
GACGGAGCCT GTGCAGCAGA GCCCACCAGC AGCGGCCCTG CCGAAGGGTC GCAGGATTCC 
GTGTGCACTG CTGGTTCTTT ACCTGTGGTA CAGGTGCCCA GTGGGGAGGA GGAAGGGCCA 
GGGGCAGAAT CGGCCACTGT ACCTGACCAG GAGGAGCTGG GGGAGGTCTG GCAGCGGAGG 
GGGAGCCTGG AGGGAGCTGC AGCTGCTGCC GAGAGCCCTC CGCAAGAAGA GGGCAGTGCT 
GGGGAGGCCC AAGGCACCTG TGAAGGGGCA ACTGCCCAGG AGGAGGGCGC TACTGGAGGT 
TCTCAAGCCA ACGGCCACCA GCCACTGCGA TCACTACCTT CAGTGCGCCA GGATGTTAGC 
CGGTACCAGA GGGTGGACGA GGCTCTTCCA CCAAACTGGG AGGCACGCAT TGACAGCCAC 
GGCAGGATCT TCTACGTGGA TCACGTAAAC AGAACCACAA CGTGGCAGCG ACCGACAGCT 
CCCCCAGCCC CGCAGGTGCT GCAGAGATCT AACTCCATAC AGCAAATGGA GCAGCTGAAC 
CGGCGATATC AGAGCATCCG CAGAACCATG ACCAATGAGA GGCCTGAGGA AAATACCAAT 
GCTATCGATG GGGCAGGGGA GGAAACTGAC TTTCACCAAG CCAGTGCAGA TTTCCGACGG 
GAGAACATCC TGCCTCACTC TACCTCCAGA TCCAGGCTCA CGTTGCTGTT ACAGTCTCCC 
CCCGTGAAGT TCCTCATCAG CCCAGAGTTC TTCACCGTGC TGCATTCTAA CCCTAGTGCC 
TACCGCATGT TTACAAACAA CACGTGTTTG AAGCACATGA TCACCAAAGT CCGGCGGGAC 
ACCCACCACT TTGAACGCTA CCAGCATAAC CGGGACCTTG TGGGATTCCT CAACATGTTT 
GCCAACAAAC AGCTAGAGCT GCCGCGGGGA TGGGAAATGA AACATGATCA CCAGGGCAAG 
GCGTTCTTTG TGGACCACAA CTCCCGTACC ACCACTTTCA TTGATCCCCG GCTCCCACTT 
CAGAGCAGTA GACCTACAAG TGCACTGGTT CATCGGCAAC ACCTGACAAG GCAACGCAGC 
CACAGTGCGG GTGAGGTAGG AGAAGATTCT CGACATGCAG GACCACCAGT TCTTCCCAGG 
CCATCCAGTA CATTCAATAC AGTCAGTAGG CCACAGTACC AGGACACGGT TCCAGTGGCT 
TACAATGACA AGATTGTTGC ATTTCTACGT CAACCCAATA TCTTTGAAAT TCTACAGGAG 
CGTCAACCTG ATCTTACCAG GAATCACTCA CTCAGGGAGA AGATCCAATT TATCCGAACT 
GAAGGGACTC CAGGATTGGT GCGCCTTTCA AGCGATGCAG ACCTCGTTAT GTTGCTGAGC 
TTATTTGAAG AAGAGATAAT GTCATATGTG CCTCCACATG CCTTACTCTA CCCCAGCTAC 
TGTCAGTCCC CACGTGGCTC TCCCGTGTCA TCTCCTCAGA ACTCACCAGG TACCCAGCGT 
GCCAATGCCC GGGCTCCAGC CCCTTACAAG CGGGATTTCG AAGCCAAACT GAGGAACTTT 
TACAGGAAGT TAGAGACTAA AGGATATGGA CAAGGCCCAG GGAAGTTAAA GTTAATTATC 
CGAAGAGATC ACTTACTAGA AGATGCTTTT AATCAGATTA TGGGCTACTC CAGAAAAGAC 
CTGCAGAGAA ATAAGCTATA TGTCACCTTC GTCGGGGAGG AAGGGCTGGA TTACAGTGGG 
CCTTCCAGAG AGTTTTTCTT CCTGGTATCC AGAGAACTCT TTAACCCATA TTATGGCTTA 
TTTGAATATT CAGCCAATGA CACATACACA GTACAAATAA GTCCTATGTC TGCTTTTGTA 
GACAATCACC ATGAATGGTT CCGATTCAGT GGTAGGATCC TTGGTCTTGC ACTAATACAC 
CAGTATTTGT TGGATGCCTT CTTCACACGG CCCTTTTATA AGGCTCTTCT CAGAATTCTA 
TGTGACCTGA GTGATCTAGA ATACCTTGAT GAAGAGTTCC ATCAGAGCCT GCAGTGGATG 
AAAGACAATG ATATCCATGA CATCCTAGAC CTCACGTTCA CTGTGAACGA AGAAGTATTT 
GGGCAGATAA CTGAACGAGA ATTAAAGCCT GGGGGTGCCA ATATCCCAGT TACAGAGAAG 
AACAAGAAGG AGTACATCGA GAGGATGGTG AAGTGGAGGA TTGAGAGGGG TGTTGTACAA 
CAAACAGAGA GTTTAGTGCG TGGCTTCTAT GAGGTGGTGG ATGCCAGGCT GGTATCTGTA 
TTTGATGCAA GAGAACTGGA ATTGGTCATC GCAGGCACAG CTGAAATAGA CCTAAGTGAT 
TGGAGAAACA ACACAGAATA TAGAGGAGGA TACCATGACA ATCATATTGT AATTCGGTGG 
TTCTGGGCTG CTGTGGAGAG ATTCAACAAT GAACAACGAC TAAGGTTGTT ACAGTTTGTT 
ACGGGCACAT CCAGCATTCC CTACGAAGGA TTTGCTTCTC TCCGAGGGAG TAACGGCCCA 
AGAAGATTCT GTGTGGAGAA ATGGGGGAAA ATCACTGCTC TTCCCAGAGC GCACACATGT 
TTTAACCGTC TGGATCTGCC CCCCTACCCA TCCTTTTCCA TGCTTTATGA GAAACTGTTG 
ACAGCAGTTG AAGAAACCAG TACTTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007963908.1
CDS	184..4902
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQLCSNGPVSEDSAADGAPKHSFRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTHEAASLEDKPENLPELAESSLPAGRAPEEGEGGPESQPSADQASAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSVCTAGSLPVVQVPSGEEEGPGAESATVPDQEELGEVWQRRGSLEGAAAAAESPPQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEETDFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDTVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLYPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_007965717.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007965717.1

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTACTT TTTGTGCGGC GTCGAAATCC CCAGATGCGG 
TACACGCTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATTAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGGGCCCTG CGAGCCACGA CCCCCTGCAT CACCGTGAAG 
AACCCGGCTG TAATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TCGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAGGCTA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT TCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAATTCTG TGAATGGAGA CTTAGGTAGC CCTTCCGATG ACGAGGACAT GCCAGGGAGC 
CATCACGACA GCCAGTTGTG CTCTAATGGG CCAGTTTCTG AGGACAGCGC TGCCGATGGG 
GCGCCCAAAC ATTCATTTAG GACTAGCTCC ACGCTGGAAA TAGACACCGA GGAATTAACT 
TCCACTTCTT CAAGGACCTC ACCTCCCAGA GGACGTCAGG ATTCACTCAA TGATTACTTA 
GATGCTATCG AACACAACGG CCACTCCAGG CCAGGGACAG CGACCTGCTC TGAGAGGTCC 
ATGGGCGCCT CTCCGAAACT GAGGAGTAGT TTTCCCACCG ACACAAGACT CAATGCCATG 
CTTCATATTG ACTCAGATGA AGAAGATCAC GAGTTCCAGC AAGACCTGGG TTACCCATCT 
TCCTTAGAGG AGGAGGGAGG CCTGATCATG TTTAGCAGGG CATCCAGAGC TGATGATGGA 
AGCCTGACAT CTCAGACAAA ACTGGAGGAC AACCCTGTTG AGAATGAGGA AGCCTCCACA 
CACGAAGCCG CTTCCTTGGA GGATAAGCCA GAAAATCTTC CAGAGCTTGC AGAAAGCTCC 
TTACCTGCAG GCCGAGCCCC AGAGGAAGGT GAAGGCGGCC CAGAGTCTCA ACCCAGTGCT 
GACCAGGCCA GTGCTGAACT GTGTGGCTCT CAAGAGGTAG ATCAGCCCAC AAGTGGGGCA 
GACACGGGGA CTTCTGATGC ATCAGGAGGA AGCCGAAGAG CTGTCAGTGA GACCGAGTCT 
CTCGATCAGG GCTCCGAGCC TTCCCAGGTG TCCTCTGAAA CAGAACCCAG TGATCCTGCC 
AGGACAGAGA GTGTGAGCGA AGCCAGCACC AGGCCCGAGG GAGAGAGTGA CCTGGAATGC 
GCTGACAGCT CTTGCAATGA GAGTGTGACC ACGCAGCTGT CCTCTGTGGA GACGCGGTGC 
TCCTCTCTTG AGAGTGCACG GTTTCCTGAA ACCCCAGCCT TCTCTTCTCA GGAGGAGGAA 
GACGGAGCCT GTGCAGCAGA GCCCACCAGC AGCGGCCCTG CCGAAGGGTC GCAGGATTCC 
GTGTGCACTG CTGGTTCTTT ACCTGTGGTA CAGGTGCCCA GTGGGGAGGA GGAAGGGCCA 
GGGGCAGAAT CGGCCACTGT ACCTGACCAG GAGGAGCTGG GGGAGGTCTG GCAGCGGAGG 
GGGAGCCTGG AGGGAGCTGC AGCTGCTGCC GAGAGCCCTC CGCAAGAAGA GGGCAGTGCT 
GGGGAGGCCC AAGGCACCTG TGAAGGGGCA ACTGCCCAGG AGGAGGGCGC TACTGGAGGT 
TCTCAAGCCA ACGGCCACCA GCCACTGCGA TCACTACCTT CAGTGCGCCA GGATGTTAGC 
CGGTACCAGA GGGTGGACGA GGCTCTTCCA CCAAACTGGG AGGCACGCAT TGACAGCCAC 
GGCAGGATCT TCTACGTGGA TCACGTAAAC AGAACCACAA CGTGGCAGCG ACCGACAGCT 
CCCCCAGCCC CGCAGGTGCT GCAGAGATCT AACTCCATAC AGCAAATGGA GCAGCTGAAC 
CGGCGATATC AGAGCATCCG CAGAACCATG ACCAATGAGA GGCCTGAGGA AAATACCAAT 
GCTATCGATG GGGCAGGGGA GGAAACTGAC TTTCACCAAG CCAGTGCAGA TTTCCGACGG 
GAGAACATCC TGCCTCACTC TACCTCCAGA TCCAGGCTCA CGTTGCTGTT ACAGTCTCCC 
CCCGTGAAGT TCCTCATCAG CCCAGAGTTC TTCACCGTGC TGCATTCTAA CCCTAGTGCC 
TACCGCATGT TTACAAACAA CACGTGTTTG AAGCACATGA TCACCAAAGT CCGGCGGGAC 
ACCCACCACT TTGAACGCTA CCAGCATAAC CGGGACCTTG TGGGATTCCT CAACATGTTT 
GCCAACAAAC AGCTAGAGCT GCCGCGGGGA TGGGAAATGA AACATGATCA CCAGGGCAAG 
GCGTTCTTTG TGGACCACAA CTCCCGTACC ACCACTTTCA TTGATCCCCG GCTCCCACTT 
CAGAGCAGTA GACCTACAAG TGCACTGGTT CATCGGCAAC ACCTGACAAG GCAACGCAGC 
CACAGTGCGG GTGAGGTAGG AGAAGATTCT CGACATGCAG GACCACCAGT TCTTCCCAGG 
CCATCCAGTA CATTCAATAC AGTCAGTAGG CCACAGTACC AGGACACGGT TCCAGTGGCT 
TACAATGACA AGATTGTTGC ATTTCTACGT CAACCCAATA TCTTTGAAAT TCTACAGGAG 
CGTCAACCTG ATCTTACCAG GAATCACTCA CTCAGGGAGA AGATCCAATT TATCCGAACT 
GAAGGGACTC CAGGATTGGT GCGCCTTTCA AGCGATGCAG ACCTCGTTAT GTTGCTGAGC 
TTATTTGAAG AAGAGATAAT GTCATATGTG CCTCCACATG CCTTACTCTA CCCCAGCTAC 
TGTCAGTCCC CACGTGGCTC TCCCGTGTCA TCTCCTCAGA ACTCACCAGG TACCCAGCGT 
GCCAATGCCC GGGCTCCAGC CCCTTACAAG CGGGATTTCG AAGCCAAACT GAGGAACTTT 
TACAGGAAGT TAGAGACTAA AGGATATGGA CAAGGCCCAG GGAAGTTAAA GTTAATTATC 
CGAAGAGATC ACTTACTAGA AGATGCTTTT AATCAGATTA TGGGCTACTC CAGAAAAGAC 
CTGCAGAGAA ATAAGCTATA TGTCACCTTC GTCGGGGAGG AAGGGCTGGA TTACAGTGGG 
CCTTCCAGAG AGTTTTTCTT CCTGGTATCC AGAGAACTCT TTAACCCATA TTATGGCTTA 
TTTGAATATT CAGCCAATGA CACATACACA GTACAAATAA GTCCTATGTC TGCTTTTGTA 
GACAATCACC ATGAATGGTT CCGATTCAGT GGTAGGATCC TTGGTCTTGC ACTAATACAC 
CAGTATTTGT TGGATGCCTT CTTCACACGG CCCTTTTATA AGGCTCTTCT CAGAATTCTA 
TGTGACCTGA GTGATCTAGA ATACCTTGAT GAAGAGTTCC ATCAGAGCCT GCAGTGGATG 
AAAGACAATG ATATCCATGA CATCCTAGAC CTCACGTTCA CTGTGAACGA AGAAGTATTT 
GGGCAGATAA CTGAACGAGA ATTAAAGCCT GGGGGTGCCA ATATCCCAGT TACAGAGAAG 
AACAAGAAGG AGTACATCGA GAGGATGGTG AAGTGGAGGA TTGAGAGGGG TGTTGTACAA 
CAAACAGAGA GTTTAGTGCG TGGCTTCTAT GAGGTGGTGG ATGCCAGGCT GGTATCTGTA 
TTTGATGCAA GAGAACTGGA ATTGGTCATC GCAGGCACAG CTGAAATAGA CCTAAGTGAT 
TGGAGAAACA ACACAGAATA TAGAGGAGGA TACCATGACA ATCATATTGT AATTCGGTGG 
TTCTGGGCTG CTGTGGAGAG ATTCAACAAT GAACAACGAC TAAGGTTGTT ACAGTTTGTT 
ACGGGCACAT CCAGCATTCC CTACGAAGGA TTTGCTTCTC TCCGAGGGAG TAACGGCCCA 
AGAAGATTCT GTGTGGAGAA ATGGGGGAAA ATCACTGCTC TTCCCAGAGC GCACACATGT 
TTTAACCGTC TGGATCTGCC CCCCTACCCA TCCTTTTCCA TGCTTTATGA GAAACTGTTG 
ACAGCAGTTG AAGAAACCAG TACTTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_007965716.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007963907.1 Chlorocebus sabaeus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

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CloneID	OCh94920
Clone ID Related Accession (Same CDS sequence)	XM_007965715.1 , XM_007965717.1 , XM_007965716.1
Accession Version	XM_007965716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735252.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGTT CAGCCCGGGA GCACCTACTT TTTGTGCGGC GTCGAAATCC CCAGATGCGG 
TACACGCTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATTAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGGGCCCTG CGAGCCACGA CCCCCTGCAT CACCGTGAAG 
AACCCGGCTG TAATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TCGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAGGCTA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT TCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAATTCTG TGAATGGAGA CTTAGGTAGC CCTTCCGATG ACGAGGACAT GCCAGGGAGC 
CATCACGACA GCCAGTTGTG CTCTAATGGG CCAGTTTCTG AGGACAGCGC TGCCGATGGG 
GCGCCCAAAC ATTCATTTAG GACTAGCTCC ACGCTGGAAA TAGACACCGA GGAATTAACT 
TCCACTTCTT CAAGGACCTC ACCTCCCAGA GGACGTCAGG ATTCACTCAA TGATTACTTA 
GATGCTATCG AACACAACGG CCACTCCAGG CCAGGGACAG CGACCTGCTC TGAGAGGTCC 
ATGGGCGCCT CTCCGAAACT GAGGAGTAGT TTTCCCACCG ACACAAGACT CAATGCCATG 
CTTCATATTG ACTCAGATGA AGAAGATCAC GAGTTCCAGC AAGACCTGGG TTACCCATCT 
TCCTTAGAGG AGGAGGGAGG CCTGATCATG TTTAGCAGGG CATCCAGAGC TGATGATGGA 
AGCCTGACAT CTCAGACAAA ACTGGAGGAC AACCCTGTTG AGAATGAGGA AGCCTCCACA 
CACGAAGCCG CTTCCTTGGA GGATAAGCCA GAAAATCTTC CAGAGCTTGC AGAAAGCTCC 
TTACCTGCAG GCCGAGCCCC AGAGGAAGGT GAAGGCGGCC CAGAGTCTCA ACCCAGTGCT 
GACCAGGCCA GTGCTGAACT GTGTGGCTCT CAAGAGGTAG ATCAGCCCAC AAGTGGGGCA 
GACACGGGGA CTTCTGATGC ATCAGGAGGA AGCCGAAGAG CTGTCAGTGA GACCGAGTCT 
CTCGATCAGG GCTCCGAGCC TTCCCAGGTG TCCTCTGAAA CAGAACCCAG TGATCCTGCC 
AGGACAGAGA GTGTGAGCGA AGCCAGCACC AGGCCCGAGG GAGAGAGTGA CCTGGAATGC 
GCTGACAGCT CTTGCAATGA GAGTGTGACC ACGCAGCTGT CCTCTGTGGA GACGCGGTGC 
TCCTCTCTTG AGAGTGCACG GTTTCCTGAA ACCCCAGCCT TCTCTTCTCA GGAGGAGGAA 
GACGGAGCCT GTGCAGCAGA GCCCACCAGC AGCGGCCCTG CCGAAGGGTC GCAGGATTCC 
GTGTGCACTG CTGGTTCTTT ACCTGTGGTA CAGGTGCCCA GTGGGGAGGA GGAAGGGCCA 
GGGGCAGAAT CGGCCACTGT ACCTGACCAG GAGGAGCTGG GGGAGGTCTG GCAGCGGAGG 
GGGAGCCTGG AGGGAGCTGC AGCTGCTGCC GAGAGCCCTC CGCAAGAAGA GGGCAGTGCT 
GGGGAGGCCC AAGGCACCTG TGAAGGGGCA ACTGCCCAGG AGGAGGGCGC TACTGGAGGT 
TCTCAAGCCA ACGGCCACCA GCCACTGCGA TCACTACCTT CAGTGCGCCA GGATGTTAGC 
CGGTACCAGA GGGTGGACGA GGCTCTTCCA CCAAACTGGG AGGCACGCAT TGACAGCCAC 
GGCAGGATCT TCTACGTGGA TCACGTAAAC AGAACCACAA CGTGGCAGCG ACCGACAGCT 
CCCCCAGCCC CGCAGGTGCT GCAGAGATCT AACTCCATAC AGCAAATGGA GCAGCTGAAC 
CGGCGATATC AGAGCATCCG CAGAACCATG ACCAATGAGA GGCCTGAGGA AAATACCAAT 
GCTATCGATG GGGCAGGGGA GGAAACTGAC TTTCACCAAG CCAGTGCAGA TTTCCGACGG 
GAGAACATCC TGCCTCACTC TACCTCCAGA TCCAGGCTCA CGTTGCTGTT ACAGTCTCCC 
CCCGTGAAGT TCCTCATCAG CCCAGAGTTC TTCACCGTGC TGCATTCTAA CCCTAGTGCC 
TACCGCATGT TTACAAACAA CACGTGTTTG AAGCACATGA TCACCAAAGT CCGGCGGGAC 
ACCCACCACT TTGAACGCTA CCAGCATAAC CGGGACCTTG TGGGATTCCT CAACATGTTT 
GCCAACAAAC AGCTAGAGCT GCCGCGGGGA TGGGAAATGA AACATGATCA CCAGGGCAAG 
GCGTTCTTTG TGGACCACAA CTCCCGTACC ACCACTTTCA TTGATCCCCG GCTCCCACTT 
CAGAGCAGTA GACCTACAAG TGCACTGGTT CATCGGCAAC ACCTGACAAG GCAACGCAGC 
CACAGTGCGG GTGAGGTAGG AGAAGATTCT CGACATGCAG GACCACCAGT TCTTCCCAGG 
CCATCCAGTA CATTCAATAC AGTCAGTAGG CCACAGTACC AGGACACGGT TCCAGTGGCT 
TACAATGACA AGATTGTTGC ATTTCTACGT CAACCCAATA TCTTTGAAAT TCTACAGGAG 
CGTCAACCTG ATCTTACCAG GAATCACTCA CTCAGGGAGA AGATCCAATT TATCCGAACT 
GAAGGGACTC CAGGATTGGT GCGCCTTTCA AGCGATGCAG ACCTCGTTAT GTTGCTGAGC 
TTATTTGAAG AAGAGATAAT GTCATATGTG CCTCCACATG CCTTACTCTA CCCCAGCTAC 
TGTCAGTCCC CACGTGGCTC TCCCGTGTCA TCTCCTCAGA ACTCACCAGG TACCCAGCGT 
GCCAATGCCC GGGCTCCAGC CCCTTACAAG CGGGATTTCG AAGCCAAACT GAGGAACTTT 
TACAGGAAGT TAGAGACTAA AGGATATGGA CAAGGCCCAG GGAAGTTAAA GTTAATTATC 
CGAAGAGATC ACTTACTAGA AGATGCTTTT AATCAGATTA TGGGCTACTC CAGAAAAGAC 
CTGCAGAGAA ATAAGCTATA TGTCACCTTC GTCGGGGAGG AAGGGCTGGA TTACAGTGGG 
CCTTCCAGAG AGTTTTTCTT CCTGGTATCC AGAGAACTCT TTAACCCATA TTATGGCTTA 
TTTGAATATT CAGCCAATGA CACATACACA GTACAAATAA GTCCTATGTC TGCTTTTGTA 
GACAATCACC ATGAATGGTT CCGATTCAGT GGTAGGATCC TTGGTCTTGC ACTAATACAC 
CAGTATTTGT TGGATGCCTT CTTCACACGG CCCTTTTATA AGGCTCTTCT CAGAATTCTA 
TGTGACCTGA GTGATCTAGA ATACCTTGAT GAAGAGTTCC ATCAGAGCCT GCAGTGGATG 
AAAGACAATG ATATCCATGA CATCCTAGAC CTCACGTTCA CTGTGAACGA AGAAGTATTT 
GGGCAGATAA CTGAACGAGA ATTAAAGCCT GGGGGTGCCA ATATCCCAGT TACAGAGAAG 
AACAAGAAGG AGTACATCGA GAGGATGGTG AAGTGGAGGA TTGAGAGGGG TGTTGTACAA 
CAAACAGAGA GTTTAGTGCG TGGCTTCTAT GAGGTGGTGG ATGCCAGGCT GGTATCTGTA 
TTTGATGCAA GAGAACTGGA ATTGGTCATC GCAGGCACAG CTGAAATAGA CCTAAGTGAT 
TGGAGAAACA ACACAGAATA TAGAGGAGGA TACCATGACA ATCATATTGT AATTCGGTGG 
TTCTGGGCTG CTGTGGAGAG ATTCAACAAT GAACAACGAC TAAGGTTGTT ACAGTTTGTT 
ACGGGCACAT CCAGCATTCC CTACGAAGGA TTTGCTTCTC TCCGAGGGAG TAACGGCCCA 
AGAAGATTCT GTGTGGAGAA ATGGGGGAAA ATCACTGCTC TTCCCAGAGC GCACACATGT 
TTTAACCGTC TGGATCTGCC CCCCTACCCA TCCTTTTCCA TGCTTTATGA GAAACTGTTG 
ACAGCAGTTG AAGAAACCAG TACTTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007963907.1
CDS	108..4826
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQLCSNGPVSEDSAADGAPKHSFRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTHEAASLEDKPENLPELAESSLPAGRAPEEGEGGPESQPSADQASAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSVCTAGSLPVVQVPSGEEEGPGAESATVPDQEELGEVWQRRGSLEGAAAAAESPPQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEETDFHQASADFRRENILPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDTVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLYPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_007965716.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007965716.1

ORF Insert Sequence:

ATGGCTAGTT CAGCCCGGGA GCACCTACTT TTTGTGCGGC GTCGAAATCC CCAGATGCGG 
TACACGCTGA GCCCAGAGAA CCTCCAGAGC CTCGCCGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTGC AGCGGGCCAA CAGCGACACC GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTAACTGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACCCA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATTAA AATCTGTTTT 
AAATATTACC ACGGCATTAG TGGGGCCCTG CGAGCCACGA CCCCCTGCAT CACCGTGAAG 
AACCCGGCTG TAATGATGGG GGCAGAAGGC ATGGAGGGAG GTGCTTCAGG AAACCTGCAT 
TCTCGAAAAC TCGTTAGCTT TACATTGTCA GATCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGACGGTCTA CTATCATCAG TAACACCACC 
AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGACAGGCTA TCGGTGATCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT TCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAATTCTG TGAATGGAGA CTTAGGTAGC CCTTCCGATG ACGAGGACAT GCCAGGGAGC 
CATCACGACA GCCAGTTGTG CTCTAATGGG CCAGTTTCTG AGGACAGCGC TGCCGATGGG 
GCGCCCAAAC ATTCATTTAG GACTAGCTCC ACGCTGGAAA TAGACACCGA GGAATTAACT 
TCCACTTCTT CAAGGACCTC ACCTCCCAGA GGACGTCAGG ATTCACTCAA TGATTACTTA 
GATGCTATCG AACACAACGG CCACTCCAGG CCAGGGACAG CGACCTGCTC TGAGAGGTCC 
ATGGGCGCCT CTCCGAAACT GAGGAGTAGT TTTCCCACCG ACACAAGACT CAATGCCATG 
CTTCATATTG ACTCAGATGA AGAAGATCAC GAGTTCCAGC AAGACCTGGG TTACCCATCT 
TCCTTAGAGG AGGAGGGAGG CCTGATCATG TTTAGCAGGG CATCCAGAGC TGATGATGGA 
AGCCTGACAT CTCAGACAAA ACTGGAGGAC AACCCTGTTG AGAATGAGGA AGCCTCCACA 
CACGAAGCCG CTTCCTTGGA GGATAAGCCA GAAAATCTTC CAGAGCTTGC AGAAAGCTCC 
TTACCTGCAG GCCGAGCCCC AGAGGAAGGT GAAGGCGGCC CAGAGTCTCA ACCCAGTGCT 
GACCAGGCCA GTGCTGAACT GTGTGGCTCT CAAGAGGTAG ATCAGCCCAC AAGTGGGGCA 
GACACGGGGA CTTCTGATGC ATCAGGAGGA AGCCGAAGAG CTGTCAGTGA GACCGAGTCT 
CTCGATCAGG GCTCCGAGCC TTCCCAGGTG TCCTCTGAAA CAGAACCCAG TGATCCTGCC 
AGGACAGAGA GTGTGAGCGA AGCCAGCACC AGGCCCGAGG GAGAGAGTGA CCTGGAATGC 
GCTGACAGCT CTTGCAATGA GAGTGTGACC ACGCAGCTGT CCTCTGTGGA GACGCGGTGC 
TCCTCTCTTG AGAGTGCACG GTTTCCTGAA ACCCCAGCCT TCTCTTCTCA GGAGGAGGAA 
GACGGAGCCT GTGCAGCAGA GCCCACCAGC AGCGGCCCTG CCGAAGGGTC GCAGGATTCC 
GTGTGCACTG CTGGTTCTTT ACCTGTGGTA CAGGTGCCCA GTGGGGAGGA GGAAGGGCCA 
GGGGCAGAAT CGGCCACTGT ACCTGACCAG GAGGAGCTGG GGGAGGTCTG GCAGCGGAGG 
GGGAGCCTGG AGGGAGCTGC AGCTGCTGCC GAGAGCCCTC CGCAAGAAGA GGGCAGTGCT 
GGGGAGGCCC AAGGCACCTG TGAAGGGGCA ACTGCCCAGG AGGAGGGCGC TACTGGAGGT 
TCTCAAGCCA ACGGCCACCA GCCACTGCGA TCACTACCTT CAGTGCGCCA GGATGTTAGC 
CGGTACCAGA GGGTGGACGA GGCTCTTCCA CCAAACTGGG AGGCACGCAT TGACAGCCAC 
GGCAGGATCT TCTACGTGGA TCACGTAAAC AGAACCACAA CGTGGCAGCG ACCGACAGCT 
CCCCCAGCCC CGCAGGTGCT GCAGAGATCT AACTCCATAC AGCAAATGGA GCAGCTGAAC 
CGGCGATATC AGAGCATCCG CAGAACCATG ACCAATGAGA GGCCTGAGGA AAATACCAAT 
GCTATCGATG GGGCAGGGGA GGAAACTGAC TTTCACCAAG CCAGTGCAGA TTTCCGACGG 
GAGAACATCC TGCCTCACTC TACCTCCAGA TCCAGGCTCA CGTTGCTGTT ACAGTCTCCC 
CCCGTGAAGT TCCTCATCAG CCCAGAGTTC TTCACCGTGC TGCATTCTAA CCCTAGTGCC 
TACCGCATGT TTACAAACAA CACGTGTTTG AAGCACATGA TCACCAAAGT CCGGCGGGAC 
ACCCACCACT TTGAACGCTA CCAGCATAAC CGGGACCTTG TGGGATTCCT CAACATGTTT 
GCCAACAAAC AGCTAGAGCT GCCGCGGGGA TGGGAAATGA AACATGATCA CCAGGGCAAG 
GCGTTCTTTG TGGACCACAA CTCCCGTACC ACCACTTTCA TTGATCCCCG GCTCCCACTT 
CAGAGCAGTA GACCTACAAG TGCACTGGTT CATCGGCAAC ACCTGACAAG GCAACGCAGC 
CACAGTGCGG GTGAGGTAGG AGAAGATTCT CGACATGCAG GACCACCAGT TCTTCCCAGG 
CCATCCAGTA CATTCAATAC AGTCAGTAGG CCACAGTACC AGGACACGGT TCCAGTGGCT 
TACAATGACA AGATTGTTGC ATTTCTACGT CAACCCAATA TCTTTGAAAT TCTACAGGAG 
CGTCAACCTG ATCTTACCAG GAATCACTCA CTCAGGGAGA AGATCCAATT TATCCGAACT 
GAAGGGACTC CAGGATTGGT GCGCCTTTCA AGCGATGCAG ACCTCGTTAT GTTGCTGAGC 
TTATTTGAAG AAGAGATAAT GTCATATGTG CCTCCACATG CCTTACTCTA CCCCAGCTAC 
TGTCAGTCCC CACGTGGCTC TCCCGTGTCA TCTCCTCAGA ACTCACCAGG TACCCAGCGT 
GCCAATGCCC GGGCTCCAGC CCCTTACAAG CGGGATTTCG AAGCCAAACT GAGGAACTTT 
TACAGGAAGT TAGAGACTAA AGGATATGGA CAAGGCCCAG GGAAGTTAAA GTTAATTATC 
CGAAGAGATC ACTTACTAGA AGATGCTTTT AATCAGATTA TGGGCTACTC CAGAAAAGAC 
CTGCAGAGAA ATAAGCTATA TGTCACCTTC GTCGGGGAGG AAGGGCTGGA TTACAGTGGG 
CCTTCCAGAG AGTTTTTCTT CCTGGTATCC AGAGAACTCT TTAACCCATA TTATGGCTTA 
TTTGAATATT CAGCCAATGA CACATACACA GTACAAATAA GTCCTATGTC TGCTTTTGTA 
GACAATCACC ATGAATGGTT CCGATTCAGT GGTAGGATCC TTGGTCTTGC ACTAATACAC 
CAGTATTTGT TGGATGCCTT CTTCACACGG CCCTTTTATA AGGCTCTTCT CAGAATTCTA 
TGTGACCTGA GTGATCTAGA ATACCTTGAT GAAGAGTTCC ATCAGAGCCT GCAGTGGATG 
AAAGACAATG ATATCCATGA CATCCTAGAC CTCACGTTCA CTGTGAACGA AGAAGTATTT 
GGGCAGATAA CTGAACGAGA ATTAAAGCCT GGGGGTGCCA ATATCCCAGT TACAGAGAAG 
AACAAGAAGG AGTACATCGA GAGGATGGTG AAGTGGAGGA TTGAGAGGGG TGTTGTACAA 
CAAACAGAGA GTTTAGTGCG TGGCTTCTAT GAGGTGGTGG ATGCCAGGCT GGTATCTGTA 
TTTGATGCAA GAGAACTGGA ATTGGTCATC GCAGGCACAG CTGAAATAGA CCTAAGTGAT 
TGGAGAAACA ACACAGAATA TAGAGGAGGA TACCATGACA ATCATATTGT AATTCGGTGG 
TTCTGGGCTG CTGTGGAGAG ATTCAACAAT GAACAACGAC TAAGGTTGTT ACAGTTTGTT 
ACGGGCACAT CCAGCATTCC CTACGAAGGA TTTGCTTCTC TCCGAGGGAG TAACGGCCCA 
AGAAGATTCT GTGTGGAGAA ATGGGGGAAA ATCACTGCTC TTCCCAGAGC GCACACATGT 
TTTAACCGTC TGGATCTGCC CCCCTACCCA TCCTTTTCCA TGCTTTATGA GAAACTGTTG 
ACAGCAGTTG AAGAAACCAG TACTTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HECW2 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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