ORF » Species Summary » Chelonia mydas » NGLY1 cDNA ORF clone

NGLY1 cDNA ORF clone, Chelonia mydas(Green sea turtle)

The following NGLY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NGLY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCh376747 XM_007068513.2
Latest version!		 Chelonia mydas N-glycanase 1 (NGLY1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OCh77364 XM_027830671.1
Latest version!		 Chelonia mydas N-glycanase 1 (NGLY1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OCh77364 XM_007068513.1
Latest version!		 Chelonia mydas N-glycanase 1 (NGLY1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    NGLY1 ( XM_007068513.2 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_007068575.2 Chelonia mydas N-glycanase 1 (NGLY1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OCh376747
    Clone ID Related Accession (Same CDS sequence) XM_007068513.2
    Accession Version XM_007068513.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006670053.1) and transcript sequence (GGMX01202746.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2019 this sequence version replaced XM_007068513.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 148 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCGGCCG CGGGGGGTCC CTCCCCGCCA CCGCGCTCTC GAGCCGTGAG TGAGCTCTGC 
    CAGAACGCTC GAGACACCTT CCTGGAGGCC TCGCGGTTAC TGCTCACCTA CGCGGACAAT 
    ATCCTCAGAC ACCCAAATGA AGAAAAATAT AGGTCTATTC GGATTGGAAA TCCAGCGTTT 
    TCTACTCGGT TGTTACCTGT TAGAGGAGCA GTTGAATGTT TGTTTGAAAT GGGATTTGAA 
    GAGGGAGAAA CTCATCTTGT TTTTCCGAAG GAAGCCTCAA TTGAACAACT ACACAAAATC 
    CGAGATCTGA TTGCTGGAGA GAGAAATAGC AGATTAAGTG AATCAAGTCA AACCCAAAGA 
    TCAGGATCTT CTCAAACTGT AACTAATATG CAGCATATTG TACCTCAGTC TTCAAGAGCT 
    GCTGACTCAA CCCTAATCCC TGTCAATAGG CAACTACAAA CATCAGCTGC ACAATCTGCT 
    GAAATGGAGT CTGAAACAAT CTTCTTTAAA ACCCTTCAAT CAAATTTTCA GCATGTGCAA 
    GTGTATGAGA ACCAGTCTCT TCAGAAGAAA GCATTGGCTT CTATTCCAGT CCAAGCATTG 
    AAAGAGAAAG CTCAAGAAAA GTTAGCACAA GCCAAAAAAG TGGATGAAGG AACCCATGTG 
    AATGAAGATG ATTTTTTGTT GTTGGAGCTC TTGCAGTGGT TTAAGGGTGA ATTTTTTCAG 
    TGGGTGAATA ACCTTGCTTG CAGCAAATGT GGTGGTCAGA CAGAGCCTAA AGGTCTCTTA 
    TCACCTAACG ATGAAGATCT GAGATGGAAT GCAAACAGAG TTGAAAATCA TCATTGTAAC 
    CAGTGCCAGT TCAGCAATAG ATTCCCAAGG TATAATAATC CTGAGAAACT TCTGGAAACA 
    AGACGTGGGC GTTGTGGAGA GTGGGCTAAC TGTTTTACAC TGTGCTGCAG AGCTATAGGA 
    TTTGAAGCTA GATATGTCTG GGATTCAACA GATCATGTGT GGACTGAGGT ATATTCTTCA 
    TCCCAGCAGA GATGGCTTCA TTGTGACCCT TGTGAAACTG TCTGTGATAA ACCTCTTCTC 
    TATGAGATTG GCTGGGGAAA GAAGCTCTCC TATGTAATTG CATTCTCCAA GGATGAGGTT 
    GTTGATGTCA CCTGGAGATA TTCCTGTAAA CACGAAGAAT TACTTTCTAG AAGGACACAA 
    GTCAAAGAAG CAGCATTGCG TGAAACCATA GAGAGGCTTA ATAAACTGAG ACAACAATCC 
    TTGTCAGAAA GCAGAAAGAG AGTGCTCCTG GAGAGGATAA TTGTGGAGCT TGTTGAGTTC 
    ATTTCTCCCA AAACTCCTAA ACCTGGTGAA CTTGGTGGAA GGATATCTGG TTCAAAGGCT 
    TGGAGAGTAG CCAGAGGTGA AATTGGTTCA GAGAGGAGAA AAGAAGTCAT TTTTATTCCA 
    TCTGGGAAAG AGAAGACCTC TAAACTCTTC CACCTCACTT ACAATATAGT GGAAGACTGC 
    TATACACGAC TTTCCAATAA TAATGAAAAA ATCACTGGCT GGGAGGAAGG TGTGTGGAAG 
    ACCGAGTCTC TATGGAGAAA GGTTGAAACG GATTGGAAAA TGGTTTATTT AGCCCGAAAA 
    GAAGGGTCAT CCTCTTCTTA CATCTGCTGG AAGTTTGAGT GTGGGTCAGT TGGTCTAAAA 
    ATAGACAACA TTTCCATTAG AACGAACAGT CAGACATTTC ACAGTGGAAG AATAAGGTGG 
    AGATTGCGCT CCAACATAGC ACAAGTTGAT CTGACTGGAG ATAAAATTCT TCGCTCCTAT 
    TCAGATTTCT CTGATGCAAC AGAAGTTATT TTGGAAGCTG AACTAAGTGG AGGGGATGGT 
    GATATTGCAT GGCAGCACAC CCAGCTGTTT AGAGAGAGCT TAAGTAACTC TGGAGAAAAT 
    TGTTTGGAGA TAATTATAAA ACTCATTGGT CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007068575.2
    CDS140..2095
    Translation

    Target ORF information:

    RefSeq Version XM_007068513.2
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas N-glycanase 1 (NGLY1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_007068513.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCGGCCG CGGGGGGTCC CTCCCCGCCA CCGCGCTCTC GAGCCGTGAG TGAGCTCTGC 
    CAGAACGCTC GAGACACCTT CCTGGAGGCC TCGCGGTTAC TGCTCACCTA CGCGGACAAT 
    ATCCTCAGAC ACCCAAATGA AGAAAAATAT AGGTCTATTC GGATTGGAAA TCCAGCGTTT 
    TCTACTCGGT TGTTACCTGT TAGAGGAGCA GTTGAATGTT TGTTTGAAAT GGGATTTGAA 
    GAGGGAGAAA CTCATCTTGT TTTTCCGAAG GAAGCCTCAA TTGAACAACT ACACAAAATC 
    CGAGATCTGA TTGCTGGAGA GAGAAATAGC AGATTAAGTG AATCAAGTCA AACCCAAAGA 
    TCAGGATCTT CTCAAACTGT AACTAATATG CAGCATATTG TACCTCAGTC TTCAAGAGCT 
    GCTGACTCAA CCCTAATCCC TGTCAATAGG CAACTACAAA CATCAGCTGC ACAATCTGCT 
    GAAATGGAGT CTGAAACAAT CTTCTTTAAA ACCCTTCAAT CAAATTTTCA GCATGTGCAA 
    GTGTATGAGA ACCAGTCTCT TCAGAAGAAA GCATTGGCTT CTATTCCAGT CCAAGCATTG 
    AAAGAGAAAG CTCAAGAAAA GTTAGCACAA GCCAAAAAAG TGGATGAAGG AACCCATGTG 
    AATGAAGATG ATTTTTTGTT GTTGGAGCTC TTGCAGTGGT TTAAGGGTGA ATTTTTTCAG 
    TGGGTGAATA ACCTTGCTTG CAGCAAATGT GGTGGTCAGA CAGAGCCTAA AGGTCTCTTA 
    TCACCTAACG ATGAAGATCT GAGATGGAAT GCAAACAGAG TTGAAAATCA TCATTGTAAC 
    CAGTGCCAGT TCAGCAATAG ATTCCCAAGG TATAATAATC CTGAGAAACT TCTGGAAACA 
    AGACGTGGGC GTTGTGGAGA GTGGGCTAAC TGTTTTACAC TGTGCTGCAG AGCTATAGGA 
    TTTGAAGCTA GATATGTCTG GGATTCAACA GATCATGTGT GGACTGAGGT ATATTCTTCA 
    TCCCAGCAGA GATGGCTTCA TTGTGACCCT TGTGAAACTG TCTGTGATAA ACCTCTTCTC 
    TATGAGATTG GCTGGGGAAA GAAGCTCTCC TATGTAATTG CATTCTCCAA GGATGAGGTT 
    GTTGATGTCA CCTGGAGATA TTCCTGTAAA CACGAAGAAT TACTTTCTAG AAGGACACAA 
    GTCAAAGAAG CAGCATTGCG TGAAACCATA GAGAGGCTTA ATAAACTGAG ACAACAATCC 
    TTGTCAGAAA GCAGAAAGAG AGTGCTCCTG GAGAGGATAA TTGTGGAGCT TGTTGAGTTC 
    ATTTCTCCCA AAACTCCTAA ACCTGGTGAA CTTGGTGGAA GGATATCTGG TTCAAAGGCT 
    TGGAGAGTAG CCAGAGGTGA AATTGGTTCA GAGAGGAGAA AAGAAGTCAT TTTTATTCCA 
    TCTGGGAAAG AGAAGACCTC TAAACTCTTC CACCTCACTT ACAATATAGT GGAAGACTGC 
    TATACACGAC TTTCCAATAA TAATGAAAAA ATCACTGGCT GGGAGGAAGG TGTGTGGAAG 
    ACCGAGTCTC TATGGAGAAA GGTTGAAACG GATTGGAAAA TGGTTTATTT AGCCCGAAAA 
    GAAGGGTCAT CCTCTTCTTA CATCTGCTGG AAGTTTGAGT GTGGGTCAGT TGGTCTAAAA 
    ATAGACAACA TTTCCATTAG AACGAACAGT CAGACATTTC ACAGTGGAAG AATAAGGTGG 
    AGATTGCGCT CCAACATAGC ACAAGTTGAT CTGACTGGAG ATAAAATTCT TCGCTCCTAT 
    TCAGATTTCT CTGATGCAAC AGAAGTTATT TTGGAAGCTG AACTAAGTGG AGGGGATGGT 
    GATATTGCAT GGCAGCACAC CCAGCTGTTT AGAGAGAGCT TAAGTAACTC TGGAGAAAAT 
    TGTTTGGAGA TAATTATAAA ACTCATTGGT CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NGLY1 ( XM_007068513.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_007068575.1 Chelonia mydas N-glycanase 1 (NGLY1), mRNA.
    Price & Availability↑
    CloneID OCh77364
    Clone ID Related Accession (Same CDS sequence) XM_007068513.1 , XM_027830671.1
    Accession Version XM_007068513.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1728bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1394035200000
    Organism Chelonia mydas(Green sea turtle)
    Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006670053.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chelonia mydas Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGATTTG AAGAGGGAGA AACTCATCTT GTTTTTCCGA AGGAAGCCTC AATTGAACAA 
    CTACACAAAA TCCGAGATCT GATTGCTGGA GAGAGAAATA GCAGATTAAG TGAATCAAGT 
    CAAACCCAAA GATCAGGATC TTCTCAAACT GTAACTAATA TGCAGCATAT TGTACCTCAG 
    TCTTCAAGAG CTGCTGACTC AACCCTAATC CCTGTCAATA GGCAACTACA AACATCAGCT 
    GCACAATCTG CTGAAATGGA GTCTGAAACA ATCTTCTTTA AAACCCTTCA ATCAAATTTT 
    CAGCATGTGC AAGTGTATGA GAACCAGTCT CTTCAGAAGA AAGCATTGGC TTCTATTCCA 
    GTCCAAGCAT TGAAAGAGAA AGCTCAAGAA AAGTTAGCAC AAGCCAAAAA AGTGGATGAA 
    GGAACCCATG TGAATGAAGA TGATTTTTTG TTGTTGGAGC TCTTGCAGTG GTTTAAGGGT 
    GAATTTTTTC AGTGGGTGAA TAACCTTGCT TGCAGCAAAT GTGGTGGTCA GACAGAGCCT 
    AAAGGTCTCT TATCACCTAA CGATGAAGAT CTGAGATGGA ATGCAAACAG AGTTGAAAAT 
    CATCATTGTA ACCAGTGCCA GTTCAGCAAT AGATTCCCAA GGTATAATAA TCCTGAGAAA 
    CTTCTGGAAA CAAGACGTGG GCGTTGTGGA GAGTGGGCTA ACTGTTTTAC ACTGTGCTGC 
    AGAGCTATAG GATTTGAAGC TAGATATGTC TGGGATTCAA CAGATCATGT GTGGACTGAG 
    GTATATTCTT CATCCCAGCA GAGATGGCTT CATTGTGACC CTTGTGAAAC TGTCTGTGAT 
    AAACCTCTTC TCTATGAGAT TGGCTGGGGA AAGAAGCTCT CCTATGTAAT TGCATTCTCC 
    AAGGATGAGG TTGTTGATGT CACCTGGAGA TATTCCTGTA AACACGAAGA ATTACTTTCT 
    AGAAGGACAC AAGTCAAAGA AGCAGCATTG CGTGAAACCA TAGAGAGGCT TAATAAACTG 
    AGACAACAAT CCTTGTCAGA AAGCAGAAAG AGAGTGCTCC TGGAGAGGAT AATTGTGGAG 
    CTTGTTGAGT TCATTTCTCC CAAAACTCCT AAACCTGGTG AACTTGGTGG AAGGATATCT 
    GGTTCAAAGG CTTGGAGAGT AGCCAGAGGT GAAATTGGTT CAGAGAGGAG AAAAGAAGTC 
    ATTTTTATTC CATCTGGGAA AGAGAAGACC TCTAAACTCT TCCACCTCAC TTACAATATA 
    GTGGAAGACT GCTATACACG ACTTTCCAAT AATAATGAAA AAATCACTGG CTGGGAGGAA 
    GGTGTGTGGA AGACCGAGTC TCTATGGAGA AAGGTTGAAA CGGATTGGAA AATGGTTTAT 
    TTAGCCCGAA AAGAAGGGTC ATCCTCTTCT TACATCTGCT GGAAGTTTGA GTGTGGGTCA 
    GTTGGTCTAA AAATAGACAA CATTTCCATT AGAACGAACA GTCAGACATT TCACAGTGGA 
    AGAATAAGGT GGAGATTGCG CTCCAACATA GCACAAGTTG ATCTGACTGG AGATAAAATT 
    CTTCGCTCCT ATTCAGATTT CTCTGATGCA ACAGAAGTTA TTTTGGAAGC TGAACTAAGT 
    GGAGGGGATG GTGATATTGC ATGGCAGCAC ACCCAGCTGT TTAGAGAGAG CTTAAGTAAC 
    TCTGGAGAAA ATTGTTTGGA GATAATTATA AAACTCATTG GTCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007068575.1
    CDS214..1941
    Translation

    Target ORF information:

    RefSeq Version XM_007068513.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas N-glycanase 1 (NGLY1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_007068513.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGATTTG AAGAGGGAGA AACTCATCTT GTTTTTCCGA AGGAAGCCTC AATTGAACAA 
    CTACACAAAA TCCGAGATCT GATTGCTGGA GAGAGAAATA GCAGATTAAG TGAATCAAGT 
    CAAACCCAAA GATCAGGATC TTCTCAAACT GTAACTAATA TGCAGCATAT TGTACCTCAG 
    TCTTCAAGAG CTGCTGACTC AACCCTAATC CCTGTCAATA GGCAACTACA AACATCAGCT 
    GCACAATCTG CTGAAATGGA GTCTGAAACA ATCTTCTTTA AAACCCTTCA ATCAAATTTT 
    CAGCATGTGC AAGTGTATGA GAACCAGTCT CTTCAGAAGA AAGCATTGGC TTCTATTCCA 
    GTCCAAGCAT TGAAAGAGAA AGCTCAAGAA AAGTTAGCAC AAGCCAAAAA AGTGGATGAA 
    GGAACCCATG TGAATGAAGA TGATTTTTTG TTGTTGGAGC TCTTGCAGTG GTTTAAGGGT 
    GAATTTTTTC AGTGGGTGAA TAACCTTGCT TGCAGCAAAT GTGGTGGTCA GACAGAGCCT 
    AAAGGTCTCT TATCACCTAA CGATGAAGAT CTGAGATGGA ATGCAAACAG AGTTGAAAAT 
    CATCATTGTA ACCAGTGCCA GTTCAGCAAT AGATTCCCAA GGTATAATAA TCCTGAGAAA 
    CTTCTGGAAA CAAGACGTGG GCGTTGTGGA GAGTGGGCTA ACTGTTTTAC ACTGTGCTGC 
    AGAGCTATAG GATTTGAAGC TAGATATGTC TGGGATTCAA CAGATCATGT GTGGACTGAG 
    GTATATTCTT CATCCCAGCA GAGATGGCTT CATTGTGACC CTTGTGAAAC TGTCTGTGAT 
    AAACCTCTTC TCTATGAGAT TGGCTGGGGA AAGAAGCTCT CCTATGTAAT TGCATTCTCC 
    AAGGATGAGG TTGTTGATGT CACCTGGAGA TATTCCTGTA AACACGAAGA ATTACTTTCT 
    AGAAGGACAC AAGTCAAAGA AGCAGCATTG CGTGAAACCA TAGAGAGGCT TAATAAACTG 
    AGACAACAAT CCTTGTCAGA AAGCAGAAAG AGAGTGCTCC TGGAGAGGAT AATTGTGGAG 
    CTTGTTGAGT TCATTTCTCC CAAAACTCCT AAACCTGGTG AACTTGGTGG AAGGATATCT 
    GGTTCAAAGG CTTGGAGAGT AGCCAGAGGT GAAATTGGTT CAGAGAGGAG AAAAGAAGTC 
    ATTTTTATTC CATCTGGGAA AGAGAAGACC TCTAAACTCT TCCACCTCAC TTACAATATA 
    GTGGAAGACT GCTATACACG ACTTTCCAAT AATAATGAAA AAATCACTGG CTGGGAGGAA 
    GGTGTGTGGA AGACCGAGTC TCTATGGAGA AAGGTTGAAA CGGATTGGAA AATGGTTTAT 
    TTAGCCCGAA AAGAAGGGTC ATCCTCTTCT TACATCTGCT GGAAGTTTGA GTGTGGGTCA 
    GTTGGTCTAA AAATAGACAA CATTTCCATT AGAACGAACA GTCAGACATT TCACAGTGGA 
    AGAATAAGGT GGAGATTGCG CTCCAACATA GCACAAGTTG ATCTGACTGG AGATAAAATT 
    CTTCGCTCCT ATTCAGATTT CTCTGATGCA ACAGAAGTTA TTTTGGAAGC TGAACTAAGT 
    GGAGGGGATG GTGATATTGC ATGGCAGCAC ACCCAGCTGT TTAGAGAGAG CTTAAGTAAC 
    TCTGGAGAAA ATTGTTTGGA GATAATTATA AAACTCATTG GTCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NGLY1 ( XM_027830671.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027686472.1 Chelonia mydas N-glycanase 1 (NGLY1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OCh77364
    Clone ID Related Accession (Same CDS sequence) XM_007068513.1 , XM_027830671.1
    Accession Version XM_027830671.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1728bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006670053.1) and transcript sequence (GGMX01202746.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 148 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGATTTG AAGAGGGAGA AACTCATCTT GTTTTTCCGA AGGAAGCCTC AATTGAACAA 
    CTACACAAAA TCCGAGATCT GATTGCTGGA GAGAGAAATA GCAGATTAAG TGAATCAAGT 
    CAAACCCAAA GATCAGGATC TTCTCAAACT GTAACTAATA TGCAGCATAT TGTACCTCAG 
    TCTTCAAGAG CTGCTGACTC AACCCTAATC CCTGTCAATA GGCAACTACA AACATCAGCT 
    GCACAATCTG CTGAAATGGA GTCTGAAACA ATCTTCTTTA AAACCCTTCA ATCAAATTTT 
    CAGCATGTGC AAGTGTATGA GAACCAGTCT CTTCAGAAGA AAGCATTGGC TTCTATTCCA 
    GTCCAAGCAT TGAAAGAGAA AGCTCAAGAA AAGTTAGCAC AAGCCAAAAA AGTGGATGAA 
    GGAACCCATG TGAATGAAGA TGATTTTTTG TTGTTGGAGC TCTTGCAGTG GTTTAAGGGT 
    GAATTTTTTC AGTGGGTGAA TAACCTTGCT TGCAGCAAAT GTGGTGGTCA GACAGAGCCT 
    AAAGGTCTCT TATCACCTAA CGATGAAGAT CTGAGATGGA ATGCAAACAG AGTTGAAAAT 
    CATCATTGTA ACCAGTGCCA GTTCAGCAAT AGATTCCCAA GGTATAATAA TCCTGAGAAA 
    CTTCTGGAAA CAAGACGTGG GCGTTGTGGA GAGTGGGCTA ACTGTTTTAC ACTGTGCTGC 
    AGAGCTATAG GATTTGAAGC TAGATATGTC TGGGATTCAA CAGATCATGT GTGGACTGAG 
    GTATATTCTT CATCCCAGCA GAGATGGCTT CATTGTGACC CTTGTGAAAC TGTCTGTGAT 
    AAACCTCTTC TCTATGAGAT TGGCTGGGGA AAGAAGCTCT CCTATGTAAT TGCATTCTCC 
    AAGGATGAGG TTGTTGATGT CACCTGGAGA TATTCCTGTA AACACGAAGA ATTACTTTCT 
    AGAAGGACAC AAGTCAAAGA AGCAGCATTG CGTGAAACCA TAGAGAGGCT TAATAAACTG 
    AGACAACAAT CCTTGTCAGA AAGCAGAAAG AGAGTGCTCC TGGAGAGGAT AATTGTGGAG 
    CTTGTTGAGT TCATTTCTCC CAAAACTCCT AAACCTGGTG AACTTGGTGG AAGGATATCT 
    GGTTCAAAGG CTTGGAGAGT AGCCAGAGGT GAAATTGGTT CAGAGAGGAG AAAAGAAGTC 
    ATTTTTATTC CATCTGGGAA AGAGAAGACC TCTAAACTCT TCCACCTCAC TTACAATATA 
    GTGGAAGACT GCTATACACG ACTTTCCAAT AATAATGAAA AAATCACTGG CTGGGAGGAA 
    GGTGTGTGGA AGACCGAGTC TCTATGGAGA AAGGTTGAAA CGGATTGGAA AATGGTTTAT 
    TTAGCCCGAA AAGAAGGGTC ATCCTCTTCT TACATCTGCT GGAAGTTTGA GTGTGGGTCA 
    GTTGGTCTAA AAATAGACAA CATTTCCATT AGAACGAACA GTCAGACATT TCACAGTGGA 
    AGAATAAGGT GGAGATTGCG CTCCAACATA GCACAAGTTG ATCTGACTGG AGATAAAATT 
    CTTCGCTCCT ATTCAGATTT CTCTGATGCA ACAGAAGTTA TTTTGGAAGC TGAACTAAGT 
    GGAGGGGATG GTGATATTGC ATGGCAGCAC ACCCAGCTGT TTAGAGAGAG CTTAAGTAAC 
    TCTGGAGAAA ATTGTTTGGA GATAATTATA AAACTCATTG GTCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027686472.1
    CDS418..2145
    Translation

    Target ORF information:

    RefSeq Version XM_027830671.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas N-glycanase 1 (NGLY1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027830671.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGATTTG AAGAGGGAGA AACTCATCTT GTTTTTCCGA AGGAAGCCTC AATTGAACAA 
    CTACACAAAA TCCGAGATCT GATTGCTGGA GAGAGAAATA GCAGATTAAG TGAATCAAGT 
    CAAACCCAAA GATCAGGATC TTCTCAAACT GTAACTAATA TGCAGCATAT TGTACCTCAG 
    TCTTCAAGAG CTGCTGACTC AACCCTAATC CCTGTCAATA GGCAACTACA AACATCAGCT 
    GCACAATCTG CTGAAATGGA GTCTGAAACA ATCTTCTTTA AAACCCTTCA ATCAAATTTT 
    CAGCATGTGC AAGTGTATGA GAACCAGTCT CTTCAGAAGA AAGCATTGGC TTCTATTCCA 
    GTCCAAGCAT TGAAAGAGAA AGCTCAAGAA AAGTTAGCAC AAGCCAAAAA AGTGGATGAA 
    GGAACCCATG TGAATGAAGA TGATTTTTTG TTGTTGGAGC TCTTGCAGTG GTTTAAGGGT 
    GAATTTTTTC AGTGGGTGAA TAACCTTGCT TGCAGCAAAT GTGGTGGTCA GACAGAGCCT 
    AAAGGTCTCT TATCACCTAA CGATGAAGAT CTGAGATGGA ATGCAAACAG AGTTGAAAAT 
    CATCATTGTA ACCAGTGCCA GTTCAGCAAT AGATTCCCAA GGTATAATAA TCCTGAGAAA 
    CTTCTGGAAA CAAGACGTGG GCGTTGTGGA GAGTGGGCTA ACTGTTTTAC ACTGTGCTGC 
    AGAGCTATAG GATTTGAAGC TAGATATGTC TGGGATTCAA CAGATCATGT GTGGACTGAG 
    GTATATTCTT CATCCCAGCA GAGATGGCTT CATTGTGACC CTTGTGAAAC TGTCTGTGAT 
    AAACCTCTTC TCTATGAGAT TGGCTGGGGA AAGAAGCTCT CCTATGTAAT TGCATTCTCC 
    AAGGATGAGG TTGTTGATGT CACCTGGAGA TATTCCTGTA AACACGAAGA ATTACTTTCT 
    AGAAGGACAC AAGTCAAAGA AGCAGCATTG CGTGAAACCA TAGAGAGGCT TAATAAACTG 
    AGACAACAAT CCTTGTCAGA AAGCAGAAAG AGAGTGCTCC TGGAGAGGAT AATTGTGGAG 
    CTTGTTGAGT TCATTTCTCC CAAAACTCCT AAACCTGGTG AACTTGGTGG AAGGATATCT 
    GGTTCAAAGG CTTGGAGAGT AGCCAGAGGT GAAATTGGTT CAGAGAGGAG AAAAGAAGTC 
    ATTTTTATTC CATCTGGGAA AGAGAAGACC TCTAAACTCT TCCACCTCAC TTACAATATA 
    GTGGAAGACT GCTATACACG ACTTTCCAAT AATAATGAAA AAATCACTGG CTGGGAGGAA 
    GGTGTGTGGA AGACCGAGTC TCTATGGAGA AAGGTTGAAA CGGATTGGAA AATGGTTTAT 
    TTAGCCCGAA AAGAAGGGTC ATCCTCTTCT TACATCTGCT GGAAGTTTGA GTGTGGGTCA 
    GTTGGTCTAA AAATAGACAA CATTTCCATT AGAACGAACA GTCAGACATT TCACAGTGGA 
    AGAATAAGGT GGAGATTGCG CTCCAACATA GCACAAGTTG ATCTGACTGG AGATAAAATT 
    CTTCGCTCCT ATTCAGATTT CTCTGATGCA ACAGAAGTTA TTTTGGAAGC TGAACTAAGT 
    GGAGGGGATG GTGATATTGC ATGGCAGCAC ACCCAGCTGT TTAGAGAGAG CTTAAGTAAC 
    TCTGGAGAAA ATTGTTTGGA GATAATTATA AAACTCATTG GTCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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