SPHKAP cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPHKAP
Entrez Gene ID	609866
Full Name	SPHK1 interactor, AKAP domain containing
General protein information	Preferred Names SPHK1 interactor, AKAP domain containing Names A-kinase anchor protein SPHKAP
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005635796.1	XP_005635853.1	A-kinase anchor protein SPHKAP isoform X2
XM_005635796.2	XP_005635853.1	A-kinase anchor protein SPHKAP isoform X2
XM_005635795.1	XP_005635852.1	A-kinase anchor protein SPHKAP isoform X1
XM_022409759.1	XP_022265467.1	A-kinase anchor protein SPHKAP isoform X3
XM_005635795.2	XP_005635852.1	A-kinase anchor protein SPHKAP isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPHKAP	XP_005635852.1
Gallus gallus (chicken)	SPHKAP	NP_001171003.1
Xenopus tropicalis (tropical clawed frog)	sphkap	XP_002933413.2
Bos taurus (cattle)	SPHKAP	XP_002685689.2
Mus musculus (house mouse)	Sphkap	NP_766018.3
Danio rerio (zebrafish)	sphkap	NP_001076514.1
Homo sapiens (human)	SPHKAP	NP_001136116.1
Pan troglodytes (chimpanzee)	SPHKAP	XP_001137841.1
Macaca mulatta (Rhesus monkey)	SPHKAP	XP_001111587.1
Rattus norvegicus (Norway rat)	Sphkap	NP_001120964.1

Gene Ontology

The following SPHKAP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPHKAP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe25756	XM_005635795.2 Latest version!	Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe25757	XM_005635796.2 Latest version!	Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe297119	XM_022409759.1 Latest version!	Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCe25757	XM_005635796.1	Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe25756	XM_005635795.1	Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPHKAP ( XM_005635795.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635852.1 Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

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CloneID	OCe25756
Clone ID Related Accession (Same CDS sequence)	XM_005635795.1 , XM_005635795.2
Accession Version	XM_005635795.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CCGGCTCTCC GTGCCCAGCC ATGTGGGTAG TTGAACCAAA GTCACCTACC 
AACCTCTCTG CTGCCTTGTC CACCAGGAGA CACTCCCAGG AATCCAGCAA TTTGGAGTCA 
CCACTGATGT ACGAGGTTTC AGCGCCACAG CAGGGCAGCG CCAGCGGCAG CTTGAGAGGC 
AGCCTGGGTA GCTCCATCAC AGCGTGTAAG AAGGTTCTTT GCAGCAACAG CCTGCTTGAG 
TCTACAGACT ACTGGTTGCA GAATCAAAGG ACACCTTGCC AGATTGGTTT TGTGGAAGAC 
AAGTCTGAAA ACTGCGCTTC TGTTTGCTTC GTGAACCTGG ATGTGAACAA GGATGCATGC 
AGCACAGAGC ACCTGCAGCA GAAATTGGTC AATGTTTCAC CAGATCTTCC AAAACTCATC 
AGCTCCATGA ACGTCCAACA GCCAAAAGAA AATGAAATTG TCCTTCTAAG TGGGTTAACA 
TCTGGAAATA TCCAGGCAGA TTTTGAAGTT TCACAGTGTC CTTGGCTGCC AGATATCTGC 
TTGGTCCAGT GTGCGAGAGG GAACAGGGCA AACAGTACCA ATTGCATCAT CTTTGAGATC 
AACAAATTTT TGATTGGTCT GGAACTGGTG CAGGAGCGGC AGCTCCACCT GGAAACAAAC 
GTCCTGAAAG TGGAGGATGA CACGAACTGT TCCCTGTCTT CCATCGAGGA GGACTTTCTC 
ACCGCCTCTG AGCACTTGGA GGAGGAAAGT GAGGTGGAAG ATTTGAGGAA CGGTTATGAA 
AACATAAGTG TCTCAGCTAA TGTTTTGGAA AATAAAAAAA CAAAGGAAGC CACTCAGGAA 
GAATGGAATT ACAACAAGGA AAAGTTACTT TATGCTTTGG AAGACAAATA TATTGGCAAA 
TATCATCCGA CGTTGATGAA AGCAGAAGGA TCTCTGGAAA AAGTAGAAGA CACCACTTTG 
CAGAGCCTGG GCACATCAGC TGAGCCATTG GGACAGAAAG GTGAAGCTGT GTGGAATGAG 
AGGCCAGCCC CAAATTATTA TTATTCGGAA AATGTTAAAG GTCACGTGGA AAAATCTCAG 
GCACCACATA CTCCCGGGGA TGCTTATTTC TCCAGGATAG TTAAGAACAG TGGGCCTCCT 
GTATATGAGC ATGACAACAG CTTACACCCA GGGGACCATG AAGATGGCAC AAACCCCCTT 
CCTCCCAAAC AAGATGGAGA AGCCACAACT GGCGAGTATG CTACAAATTT AGCAGAATCC 
GTGCTGCAAG ATGCATTTAT TCGGTTGTCC CAGTGTCAGC CTACACCTCC CCAGGAATCG 
GCAGTCAGTG TTTCTGTCGG AAATGCTGTG CTACCCAGTG GCTGTTCCAC AAAAGGTATG 
GTGGTCCCTC GGTCATGGAA TGAGCTCCCC AAAATTGTCA TTGTTCAGAG TCCAGATGGC 
AGTGACGTCG CCCCGGAGGC AGCCATCACC TCATGGTCTG ACATGGAAGT CTCTGTTGAA 
ACCTCAGGTG TCTTCTCTGG AGAGAACTCC AGCAGACACC CTCAGAGTGC TCTAGAAGTT 
GCCTTGGCCT GTGCGGCCAC TGTAATTGGA ACCATTTCCA GTCCACAGGC CACAGAAAGG 
CTCAAAATGG AGCAAGAATC TTTGGTTTCA ACGCATCCAC TGGGAGGCAG TGAACCACTG 
CAAACTCAAA TATCCCAAGT ACTCAAGGAG CCTTCCATCA GCGAATACTC CTTTCCATCT 
GCTTTGTGTG GCATGACTCA GGTGGCGAGT GCCATTGCTG TTTGTGGCCT GGGTGAAACA 
GGAGAGGCCA AGGCTCCCAG TGGCCTCTCG GGTACAGCTG AGGCCTGTGC AGTAGTTCCC 
ACACCTTGCA GTCTGGCGAC AGGGAGGAGC ATGGAGCTGG GAAATGAGGC CATTGCACGA 
GCATTGCTCA AGGAGGCCGC TCTGCTTTTA ACAAGGCCAG AATCTTGCAG CAGCGTAGGA 
GACCTCATGG AATCAATGAG CGAGAGAATC ATTGAGGCTG CTTCAAAGCC TCAGACCTTG 
TGCTCAGAAA ACGTCCTCAG GAATGAAGTA GCACAGACCC TGTCCAGTGT TATTCTGAAG 
CATTCCATTG ATGAAGCTCG CCAGAAAAGC AAAATAATTG ATCCCAACGA TACTAGGCAT 
TCTCCAGAGA CTCTTGACAC CTGGATGGAA AGTACAAATC AACTGCTCTT CAGTGTGCTT 
TGCTTCACAT TCAAGAAGAT GAGCCATTTT GTACAGCTTG GTGAGTGCCC CGCCGTCCTT 
GTTAATGAGA CCAGCAACTG GAAAGAACCA GAATTAGGCT ACTGGCCGCC TGACCAGGCT 
GCCAGTCAAG CATGGACCAA AGCCTCTGAA TATTCCAGCA GCCATCCTCT TAGCAGTCCA 
CGCAAAACTA GTCTTGTTAT CAATGATCTC GGGGATGAAG TTCATCTCAA GCCAGATAGC 
AAGGGCCCGG CGAAGCCAGG CCTGTTCAAG AACCCCATGC TGCAGTCTGA ATTGCCCTGT 
AGTCCCAGAG TGCCTGACTC TTTGGCTGCT AAAACAGCCC CCAAAGAAAT ATACCTAAAA 
AGAGCAGCGG GAGAAGATCC AGGCAACCCT CATCCTGCGC GAAGGCGCAA TGAGAAGGAG 
TACAGAAGCC CTGCAGAAGG AGAAAGGGCG CCGGCGGGGG CCCAGGGCTG CAGAGGCCCC 
CAGGATGCAG AGGACAGCGC AGGTGCCGAC ACCCAGGAGA AGTCTAACCG TGTGTTGCCC 
CTGAACAGTG AAGGTCAAGT GAACCTGTCT TGGTTAGGGA ATGATGCGCT GCTTCCTGCC 
CAACCCGTGC TGCAGGCAAC ACGTGCAGAA CTCTACTGCA TCACAGACTT TGCGGAAGAA 
TTAGCCGAGA TGATTGTCTC CATGGCAACC GAAATCGCAG CAATTTGCCT TGACAACTCA 
AATGGAAAAC AACCCTGGTT TTGTGCATGG AAGAGAGGGA ATGAGTTTCT GGTTACCCCC 
AACGTATCCT GCCGGTCCCT GAAGAGGAAG AAGGAAAGCC AGCCCGGCGG GGCCACCGTG 
AGGAAGCACA AGCCACCCCG GCTCAGCGAG ATCAAGAGGA AAACGGATGA GCACCCGGAG 
CTTAAGGAGA AGCTGATGAA CAGGGTCGTG GACGAGTCGA TGAACCTCGA GGACGTCCCA 
GATTCTGTCA ATATTTTTGC GAACGAAGTG GCCGCCAAGA TCATGAACCT AACGGACTTC 
TCCATGGTAG ACGGTGTCTG GCAAGCCCAG AGCCACCCCC GCACCCGGTT ACTCAGCGGA 
GACAGGTGGA GCCGACTGAA GGCCTCCAGC TGTGAAAGCA TTCCAGAGGA GGACTCTAAC 
GCCAGGGCTT ATGTAAATAG CCTGGGTTTC ATGAGTACCC TAAGTCAGCC AGTGAGCAGG 
GCCAGCTCTG TCTCCAAGCA GTCCAGCTGT GAGAGCATCA CTGAGGAGTT TTCCAGGTTC 
ATGGTGAACC AGATGGAAAA TGAAGGAAGA GGGTTTGAGT TACTGCTGGA TTACTATGCG 
GGCAAGAATG CCACCAGCAT CCTGAACTCA GCCATGCACC AGGCCTGCCA TAAAAATGAC 
CACCTCAGTG TGAGGCCGAG CTGTCCCTCG AAGCAGTCCA GCACCGAGAG CATAACCGAG 
GAGTTTTATA GGTACATGCT GAGGGACATT GAAAGAGAAA GCAGGGACAG CGCCTCCTCC 
AGACGAAGCA GCCAAGATTG GACAGTTGGC TTACTGTCAC CCTCTCTGCG ATCCCCATTG 
TGTTACAGAC AGTCATCTGT GCCCGATAGC CGATCCCTGG GTGCCAGGCT GACGGTGAAC 
CCACCCATCA AAGCCAACTC TTTAGATGGC TTCGCTCAAA ACAGCCAGCA AGATTTCCTA 
AGTGTACATC CAGTCAGTAG CACTTCCTCC TCGGGTCTCT GCAAATCTGA CTCTTGCTTG 
TATCGCAGAG GCGGAACTGA CCACATCACC AACATGTTAA TTCATGAGAC CTGGGCAAAC 
TCCATCAAGG CCCTCATGCA GAAGAACAAA ATTCTAGTGG ACGATGCCGA GGCAGCCGAT 
GCTGAGCCCG TTCCTGGTGG CTCTCCCATG CAAGTGGAGA AATGTGCAAA CAGATCGGCT 
TTGAGCAGAG TGCAAGGTGG GCCAGTTCTT CTTGTTCAGG AGTCTGTTGA TACCCCCAGG 
AAAAACTCCA TTACCGACAG CAAACATCCC CCAGTATCAT CTAAGAGCAA AGCTGCTCCT 
CTGACAAACC ACAACAGTTT AGATTCTAAG AAAGAATCTT CTTCGTGCCA TGGATCTGTG 
CCTGTCAGCC ACACCAAGCG GTCACTTTGC TCAAGGGAAG TGCCTTTAAT TCAGATTGAA 
ACAGATCAGA GAGAAGAGTG TGTTGGAGAA CCTGAACATT CCCCTTCCAA AAGCAGCCCC 
CTAGAGGAAG CAGAGGAGCA TTTGAAAGAT GCAAAAGTCC CAGAGGTGGC GAGGGGTGGA 
GACACTGCTG AGAGTGCCTG CCAAAGTCCT AGTGACAATC TTGATGCCAG CGATGTACCA 
GGGGCTGAAG TCTCGGCGGA AGGCAGAGCC CCCGATGAGT TCCCCAACCT TTCCGGCAGC 
AGTGGGGAGA GCACAGACAG CTGGTCCCAG CTCGCCAATG AGGAGGACAA TCCAGATGAC 
ACAAGTAGTT TTCTGCAGCT CAGTGAGCGA TCCATGAGCA ATGGCAACAG TAGTGCCACT 
AGCAGTCTTG GCACTATGGA CCTGGACATT TATCAGGAAA GCATGCCATC TTCTCCCATG 
ATTAATGAAT TGGTGGAAGA AAAGGAGATT CTTAAAGGAC AGTCAGAAAA CGTAGAGGAA 
GGTGCCTCTG GAGCGCCAAT GGGAGTAGCC AGCTCCCAGG GGAGCCTGCT GGTGATCAAC 
TTTGACCTGG AGCCAGAATG TCCTGATGCT GAGCTTCGAG CCACTCTGCA GTGGATAGCA 
GCCTCTGAAC TTGGGATCCC CACCATCTAC TTTAAGAAAT CTCAGGAAAA CAGAATTGAA 
AAGTTTCTAG ATGTTGTGCG ACTGGTTCAG CAGAAGTCCT GGAAAGTGGG AGATATCTTC 
CATGCGGTTG TTCAGTACTG CAAAATGCAT GAGGAGCAGA AGGAGGGGAC CCCAAGTCTC 
TTTGACTGGC TCTTGGAACT GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635852.1
CDS	308..5494
Translation	MATPGSPCPAMWVVEPKSPTNLSAALSTRRHSQESSNLESPLMYEVSAPQQGSASGSLRGSLGSSITACKKVLCSNSLLESTDYWLQNQRTPCQIGFVEDKSENCASVCFVNLDVNKDACSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVLLSGLTSGNIQADFEVSQCPWLPDICLVQCARGNRANSTNCIIFEINKFLIGLELVQERQLHLETNVLKVEDDTNCSLSSIEEDFLTASEHLEEESEVEDLRNGYENISVSANVLENKKTKEATQEEWNYNKEKLLYALEDKYIGKYHPTLMKAEGSLEKVEDTTLQSLGTSAEPLGQKGEAVWNERPAPNYYYSENVKGHVEKSQAPHTPGDAYFSRIVKNSGPPVYEHDNSLHPGDHEDGTNPLPPKQDGEATTGEYATNLAESVLQDAFIRLSQCQPTPPQESAVSVSVGNAVLPSGCSTKGMVVPRSWNELPKIVIVQSPDGSDVAPEAAITSWSDMEVSVETSGVFSGENSSRHPQSALEVALACAATVIGTISSPQATERLKMEQESLVSTHPLGGSEPLQTQISQVLKEPSISEYSFPSALCGMTQVASAIAVCGLGETGEAKAPSGLSGTAEACAVVPTPCSLATGRSMELGNEAIARALLKEAALLLTRPESCSSVGDLMESMSERIIEAASKPQTLCSENVLRNEVAQTLSSVILKHSIDEARQKSKIIDPNDTRHSPETLDTWMESTNQLLFSVLCFTFKKMSHFVQLGECPAVLVNETSNWKEPELGYWPPDQAASQAWTKASEYSSSHPLSSPRKTSLVINDLGDEVHLKPDSKGPAKPGLFKNPMLQSELPCSPRVPDSLAAKTAPKEIYLKRAAGEDPGNPHPARRRNEKEYRSPAEGERAPAGAQGCRGPQDAEDSAGADTQEKSNRVLPLNSEGQVNLSWLGNDALLPAQPVLQATRAELYCITDFAEELAEMIVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLVTPNVSCRSLKRKKESQPGGATVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNIFANEVAAKIMNLTDFSMVDGVWQAQSHPRTRLLSGDRWSRLKASSCESIPEEDSNARAYVNSLGFMSTLSQPVSRASSVSKQSSCESITEEFSRFMVNQMENEGRGFELLLDYYAGKNATSILNSAMHQACHKNDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERESRDSASSRRSSQDWTVGLLSPSLRSPLCYRQSSVPDSRSLGARLTVNPPIKANSLDGFAQNSQQDFLSVHPVSSTSSSGLCKSDSCLYRRGGTDHITNMLIHETWANSIKALMQKNKILVDDAEAADAEPVPGGSPMQVEKCANRSALSRVQGGPVLLVQESVDTPRKNSITDSKHPPVSSKSKAAPLTNHNSLDSKKESSSCHGSVPVSHTKRSLCSREVPLIQIETDQREECVGEPEHSPSKSSPLEEAEEHLKDAKVPEVARGGDTAESACQSPSDNLDASDVPGAEVSAEGRAPDEFPNLSGSSGESTDSWSQLANEEDNPDDTSSFLQLSERSMSNGNSSATSSLGTMDLDIYQESMPSSPMINELVEEKEILKGQSENVEEGASGAPMGVASSQGSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVRLVQQKSWKVGDIFHAVVQYCKMHEEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_005635795.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635795.1

ORF Insert Sequence:

ATGGCAACTC CCGGCTCTCC GTGCCCAGCC ATGTGGGTAG TTGAACCAAA GTCACCTACC 
AACCTCTCTG CTGCCTTGTC CACCAGGAGA CACTCCCAGG AATCCAGCAA TTTGGAGTCA 
CCACTGATGT ACGAGGTTTC AGCGCCACAG CAGGGCAGCG CCAGCGGCAG CTTGAGAGGC 
AGCCTGGGTA GCTCCATCAC AGCGTGTAAG AAGGTTCTTT GCAGCAACAG CCTGCTTGAG 
TCTACAGACT ACTGGTTGCA GAATCAAAGG ACACCTTGCC AGATTGGTTT TGTGGAAGAC 
AAGTCTGAAA ACTGCGCTTC TGTTTGCTTC GTGAACCTGG ATGTGAACAA GGATGCATGC 
AGCACAGAGC ACCTGCAGCA GAAATTGGTC AATGTTTCAC CAGATCTTCC AAAACTCATC 
AGCTCCATGA ACGTCCAACA GCCAAAAGAA AATGAAATTG TCCTTCTAAG TGGGTTAACA 
TCTGGAAATA TCCAGGCAGA TTTTGAAGTT TCACAGTGTC CTTGGCTGCC AGATATCTGC 
TTGGTCCAGT GTGCGAGAGG GAACAGGGCA AACAGTACCA ATTGCATCAT CTTTGAGATC 
AACAAATTTT TGATTGGTCT GGAACTGGTG CAGGAGCGGC AGCTCCACCT GGAAACAAAC 
GTCCTGAAAG TGGAGGATGA CACGAACTGT TCCCTGTCTT CCATCGAGGA GGACTTTCTC 
ACCGCCTCTG AGCACTTGGA GGAGGAAAGT GAGGTGGAAG ATTTGAGGAA CGGTTATGAA 
AACATAAGTG TCTCAGCTAA TGTTTTGGAA AATAAAAAAA CAAAGGAAGC CACTCAGGAA 
GAATGGAATT ACAACAAGGA AAAGTTACTT TATGCTTTGG AAGACAAATA TATTGGCAAA 
TATCATCCGA CGTTGATGAA AGCAGAAGGA TCTCTGGAAA AAGTAGAAGA CACCACTTTG 
CAGAGCCTGG GCACATCAGC TGAGCCATTG GGACAGAAAG GTGAAGCTGT GTGGAATGAG 
AGGCCAGCCC CAAATTATTA TTATTCGGAA AATGTTAAAG GTCACGTGGA AAAATCTCAG 
GCACCACATA CTCCCGGGGA TGCTTATTTC TCCAGGATAG TTAAGAACAG TGGGCCTCCT 
GTATATGAGC ATGACAACAG CTTACACCCA GGGGACCATG AAGATGGCAC AAACCCCCTT 
CCTCCCAAAC AAGATGGAGA AGCCACAACT GGCGAGTATG CTACAAATTT AGCAGAATCC 
GTGCTGCAAG ATGCATTTAT TCGGTTGTCC CAGTGTCAGC CTACACCTCC CCAGGAATCG 
GCAGTCAGTG TTTCTGTCGG AAATGCTGTG CTACCCAGTG GCTGTTCCAC AAAAGGTATG 
GTGGTCCCTC GGTCATGGAA TGAGCTCCCC AAAATTGTCA TTGTTCAGAG TCCAGATGGC 
AGTGACGTCG CCCCGGAGGC AGCCATCACC TCATGGTCTG ACATGGAAGT CTCTGTTGAA 
ACCTCAGGTG TCTTCTCTGG AGAGAACTCC AGCAGACACC CTCAGAGTGC TCTAGAAGTT 
GCCTTGGCCT GTGCGGCCAC TGTAATTGGA ACCATTTCCA GTCCACAGGC CACAGAAAGG 
CTCAAAATGG AGCAAGAATC TTTGGTTTCA ACGCATCCAC TGGGAGGCAG TGAACCACTG 
CAAACTCAAA TATCCCAAGT ACTCAAGGAG CCTTCCATCA GCGAATACTC CTTTCCATCT 
GCTTTGTGTG GCATGACTCA GGTGGCGAGT GCCATTGCTG TTTGTGGCCT GGGTGAAACA 
GGAGAGGCCA AGGCTCCCAG TGGCCTCTCG GGTACAGCTG AGGCCTGTGC AGTAGTTCCC 
ACACCTTGCA GTCTGGCGAC AGGGAGGAGC ATGGAGCTGG GAAATGAGGC CATTGCACGA 
GCATTGCTCA AGGAGGCCGC TCTGCTTTTA ACAAGGCCAG AATCTTGCAG CAGCGTAGGA 
GACCTCATGG AATCAATGAG CGAGAGAATC ATTGAGGCTG CTTCAAAGCC TCAGACCTTG 
TGCTCAGAAA ACGTCCTCAG GAATGAAGTA GCACAGACCC TGTCCAGTGT TATTCTGAAG 
CATTCCATTG ATGAAGCTCG CCAGAAAAGC AAAATAATTG ATCCCAACGA TACTAGGCAT 
TCTCCAGAGA CTCTTGACAC CTGGATGGAA AGTACAAATC AACTGCTCTT CAGTGTGCTT 
TGCTTCACAT TCAAGAAGAT GAGCCATTTT GTACAGCTTG GTGAGTGCCC CGCCGTCCTT 
GTTAATGAGA CCAGCAACTG GAAAGAACCA GAATTAGGCT ACTGGCCGCC TGACCAGGCT 
GCCAGTCAAG CATGGACCAA AGCCTCTGAA TATTCCAGCA GCCATCCTCT TAGCAGTCCA 
CGCAAAACTA GTCTTGTTAT CAATGATCTC GGGGATGAAG TTCATCTCAA GCCAGATAGC 
AAGGGCCCGG CGAAGCCAGG CCTGTTCAAG AACCCCATGC TGCAGTCTGA ATTGCCCTGT 
AGTCCCAGAG TGCCTGACTC TTTGGCTGCT AAAACAGCCC CCAAAGAAAT ATACCTAAAA 
AGAGCAGCGG GAGAAGATCC AGGCAACCCT CATCCTGCGC GAAGGCGCAA TGAGAAGGAG 
TACAGAAGCC CTGCAGAAGG AGAAAGGGCG CCGGCGGGGG CCCAGGGCTG CAGAGGCCCC 
CAGGATGCAG AGGACAGCGC AGGTGCCGAC ACCCAGGAGA AGTCTAACCG TGTGTTGCCC 
CTGAACAGTG AAGGTCAAGT GAACCTGTCT TGGTTAGGGA ATGATGCGCT GCTTCCTGCC 
CAACCCGTGC TGCAGGCAAC ACGTGCAGAA CTCTACTGCA TCACAGACTT TGCGGAAGAA 
TTAGCCGAGA TGATTGTCTC CATGGCAACC GAAATCGCAG CAATTTGCCT TGACAACTCA 
AATGGAAAAC AACCCTGGTT TTGTGCATGG AAGAGAGGGA ATGAGTTTCT GGTTACCCCC 
AACGTATCCT GCCGGTCCCT GAAGAGGAAG AAGGAAAGCC AGCCCGGCGG GGCCACCGTG 
AGGAAGCACA AGCCACCCCG GCTCAGCGAG ATCAAGAGGA AAACGGATGA GCACCCGGAG 
CTTAAGGAGA AGCTGATGAA CAGGGTCGTG GACGAGTCGA TGAACCTCGA GGACGTCCCA 
GATTCTGTCA ATATTTTTGC GAACGAAGTG GCCGCCAAGA TCATGAACCT AACGGACTTC 
TCCATGGTAG ACGGTGTCTG GCAAGCCCAG AGCCACCCCC GCACCCGGTT ACTCAGCGGA 
GACAGGTGGA GCCGACTGAA GGCCTCCAGC TGTGAAAGCA TTCCAGAGGA GGACTCTAAC 
GCCAGGGCTT ATGTAAATAG CCTGGGTTTC ATGAGTACCC TAAGTCAGCC AGTGAGCAGG 
GCCAGCTCTG TCTCCAAGCA GTCCAGCTGT GAGAGCATCA CTGAGGAGTT TTCCAGGTTC 
ATGGTGAACC AGATGGAAAA TGAAGGAAGA GGGTTTGAGT TACTGCTGGA TTACTATGCG 
GGCAAGAATG CCACCAGCAT CCTGAACTCA GCCATGCACC AGGCCTGCCA TAAAAATGAC 
CACCTCAGTG TGAGGCCGAG CTGTCCCTCG AAGCAGTCCA GCACCGAGAG CATAACCGAG 
GAGTTTTATA GGTACATGCT GAGGGACATT GAAAGAGAAA GCAGGGACAG CGCCTCCTCC 
AGACGAAGCA GCCAAGATTG GACAGTTGGC TTACTGTCAC CCTCTCTGCG ATCCCCATTG 
TGTTACAGAC AGTCATCTGT GCCCGATAGC CGATCCCTGG GTGCCAGGCT GACGGTGAAC 
CCACCCATCA AAGCCAACTC TTTAGATGGC TTCGCTCAAA ACAGCCAGCA AGATTTCCTA 
AGTGTACATC CAGTCAGTAG CACTTCCTCC TCGGGTCTCT GCAAATCTGA CTCTTGCTTG 
TATCGCAGAG GCGGAACTGA CCACATCACC AACATGTTAA TTCATGAGAC CTGGGCAAAC 
TCCATCAAGG CCCTCATGCA GAAGAACAAA ATTCTAGTGG ACGATGCCGA GGCAGCCGAT 
GCTGAGCCCG TTCCTGGTGG CTCTCCCATG CAAGTGGAGA AATGTGCAAA CAGATCGGCT 
TTGAGCAGAG TGCAAGGTGG GCCAGTTCTT CTTGTTCAGG AGTCTGTTGA TACCCCCAGG 
AAAAACTCCA TTACCGACAG CAAACATCCC CCAGTATCAT CTAAGAGCAA AGCTGCTCCT 
CTGACAAACC ACAACAGTTT AGATTCTAAG AAAGAATCTT CTTCGTGCCA TGGATCTGTG 
CCTGTCAGCC ACACCAAGCG GTCACTTTGC TCAAGGGAAG TGCCTTTAAT TCAGATTGAA 
ACAGATCAGA GAGAAGAGTG TGTTGGAGAA CCTGAACATT CCCCTTCCAA AAGCAGCCCC 
CTAGAGGAAG CAGAGGAGCA TTTGAAAGAT GCAAAAGTCC CAGAGGTGGC GAGGGGTGGA 
GACACTGCTG AGAGTGCCTG CCAAAGTCCT AGTGACAATC TTGATGCCAG CGATGTACCA 
GGGGCTGAAG TCTCGGCGGA AGGCAGAGCC CCCGATGAGT TCCCCAACCT TTCCGGCAGC 
AGTGGGGAGA GCACAGACAG CTGGTCCCAG CTCGCCAATG AGGAGGACAA TCCAGATGAC 
ACAAGTAGTT TTCTGCAGCT CAGTGAGCGA TCCATGAGCA ATGGCAACAG TAGTGCCACT 
AGCAGTCTTG GCACTATGGA CCTGGACATT TATCAGGAAA GCATGCCATC TTCTCCCATG 
ATTAATGAAT TGGTGGAAGA AAAGGAGATT CTTAAAGGAC AGTCAGAAAA CGTAGAGGAA 
GGTGCCTCTG GAGCGCCAAT GGGAGTAGCC AGCTCCCAGG GGAGCCTGCT GGTGATCAAC 
TTTGACCTGG AGCCAGAATG TCCTGATGCT GAGCTTCGAG CCACTCTGCA GTGGATAGCA 
GCCTCTGAAC TTGGGATCCC CACCATCTAC TTTAAGAAAT CTCAGGAAAA CAGAATTGAA 
AAGTTTCTAG ATGTTGTGCG ACTGGTTCAG CAGAAGTCCT GGAAAGTGGG AGATATCTTC 
CATGCGGTTG TTCAGTACTG CAAAATGCAT GAGGAGCAGA AGGAGGGGAC CCCAAGTCTC 
TTTGACTGGC TCTTGGAACT GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_005635795.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635852.1 Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

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CloneID	OCe25756
Clone ID Related Accession (Same CDS sequence)	XM_005635795.1 , XM_005635795.2
Accession Version	XM_005635795.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CCGGCTCTCC GTGCCCAGCC ATGTGGGTAG TTGAACCAAA GTCACCTACC 
AACCTCTCTG CTGCCTTGTC CACCAGGAGA CACTCCCAGG AATCCAGCAA TTTGGAGTCA 
CCACTGATGT ACGAGGTTTC AGCGCCACAG CAGGGCAGCG CCAGCGGCAG CTTGAGAGGC 
AGCCTGGGTA GCTCCATCAC AGCGTGTAAG AAGGTTCTTT GCAGCAACAG CCTGCTTGAG 
TCTACAGACT ACTGGTTGCA GAATCAAAGG ACACCTTGCC AGATTGGTTT TGTGGAAGAC 
AAGTCTGAAA ACTGCGCTTC TGTTTGCTTC GTGAACCTGG ATGTGAACAA GGATGCATGC 
AGCACAGAGC ACCTGCAGCA GAAATTGGTC AATGTTTCAC CAGATCTTCC AAAACTCATC 
AGCTCCATGA ACGTCCAACA GCCAAAAGAA AATGAAATTG TCCTTCTAAG TGGGTTAACA 
TCTGGAAATA TCCAGGCAGA TTTTGAAGTT TCACAGTGTC CTTGGCTGCC AGATATCTGC 
TTGGTCCAGT GTGCGAGAGG GAACAGGGCA AACAGTACCA ATTGCATCAT CTTTGAGATC 
AACAAATTTT TGATTGGTCT GGAACTGGTG CAGGAGCGGC AGCTCCACCT GGAAACAAAC 
GTCCTGAAAG TGGAGGATGA CACGAACTGT TCCCTGTCTT CCATCGAGGA GGACTTTCTC 
ACCGCCTCTG AGCACTTGGA GGAGGAAAGT GAGGTGGAAG ATTTGAGGAA CGGTTATGAA 
AACATAAGTG TCTCAGCTAA TGTTTTGGAA AATAAAAAAA CAAAGGAAGC CACTCAGGAA 
GAATGGAATT ACAACAAGGA AAAGTTACTT TATGCTTTGG AAGACAAATA TATTGGCAAA 
TATCATCCGA CGTTGATGAA AGCAGAAGGA TCTCTGGAAA AAGTAGAAGA CACCACTTTG 
CAGAGCCTGG GCACATCAGC TGAGCCATTG GGACAGAAAG GTGAAGCTGT GTGGAATGAG 
AGGCCAGCCC CAAATTATTA TTATTCGGAA AATGTTAAAG GTCACGTGGA AAAATCTCAG 
GCACCACATA CTCCCGGGGA TGCTTATTTC TCCAGGATAG TTAAGAACAG TGGGCCTCCT 
GTATATGAGC ATGACAACAG CTTACACCCA GGGGACCATG AAGATGGCAC AAACCCCCTT 
CCTCCCAAAC AAGATGGAGA AGCCACAACT GGCGAGTATG CTACAAATTT AGCAGAATCC 
GTGCTGCAAG ATGCATTTAT TCGGTTGTCC CAGTGTCAGC CTACACCTCC CCAGGAATCG 
GCAGTCAGTG TTTCTGTCGG AAATGCTGTG CTACCCAGTG GCTGTTCCAC AAAAGGTATG 
GTGGTCCCTC GGTCATGGAA TGAGCTCCCC AAAATTGTCA TTGTTCAGAG TCCAGATGGC 
AGTGACGTCG CCCCGGAGGC AGCCATCACC TCATGGTCTG ACATGGAAGT CTCTGTTGAA 
ACCTCAGGTG TCTTCTCTGG AGAGAACTCC AGCAGACACC CTCAGAGTGC TCTAGAAGTT 
GCCTTGGCCT GTGCGGCCAC TGTAATTGGA ACCATTTCCA GTCCACAGGC CACAGAAAGG 
CTCAAAATGG AGCAAGAATC TTTGGTTTCA ACGCATCCAC TGGGAGGCAG TGAACCACTG 
CAAACTCAAA TATCCCAAGT ACTCAAGGAG CCTTCCATCA GCGAATACTC CTTTCCATCT 
GCTTTGTGTG GCATGACTCA GGTGGCGAGT GCCATTGCTG TTTGTGGCCT GGGTGAAACA 
GGAGAGGCCA AGGCTCCCAG TGGCCTCTCG GGTACAGCTG AGGCCTGTGC AGTAGTTCCC 
ACACCTTGCA GTCTGGCGAC AGGGAGGAGC ATGGAGCTGG GAAATGAGGC CATTGCACGA 
GCATTGCTCA AGGAGGCCGC TCTGCTTTTA ACAAGGCCAG AATCTTGCAG CAGCGTAGGA 
GACCTCATGG AATCAATGAG CGAGAGAATC ATTGAGGCTG CTTCAAAGCC TCAGACCTTG 
TGCTCAGAAA ACGTCCTCAG GAATGAAGTA GCACAGACCC TGTCCAGTGT TATTCTGAAG 
CATTCCATTG ATGAAGCTCG CCAGAAAAGC AAAATAATTG ATCCCAACGA TACTAGGCAT 
TCTCCAGAGA CTCTTGACAC CTGGATGGAA AGTACAAATC AACTGCTCTT CAGTGTGCTT 
TGCTTCACAT TCAAGAAGAT GAGCCATTTT GTACAGCTTG GTGAGTGCCC CGCCGTCCTT 
GTTAATGAGA CCAGCAACTG GAAAGAACCA GAATTAGGCT ACTGGCCGCC TGACCAGGCT 
GCCAGTCAAG CATGGACCAA AGCCTCTGAA TATTCCAGCA GCCATCCTCT TAGCAGTCCA 
CGCAAAACTA GTCTTGTTAT CAATGATCTC GGGGATGAAG TTCATCTCAA GCCAGATAGC 
AAGGGCCCGG CGAAGCCAGG CCTGTTCAAG AACCCCATGC TGCAGTCTGA ATTGCCCTGT 
AGTCCCAGAG TGCCTGACTC TTTGGCTGCT AAAACAGCCC CCAAAGAAAT ATACCTAAAA 
AGAGCAGCGG GAGAAGATCC AGGCAACCCT CATCCTGCGC GAAGGCGCAA TGAGAAGGAG 
TACAGAAGCC CTGCAGAAGG AGAAAGGGCG CCGGCGGGGG CCCAGGGCTG CAGAGGCCCC 
CAGGATGCAG AGGACAGCGC AGGTGCCGAC ACCCAGGAGA AGTCTAACCG TGTGTTGCCC 
CTGAACAGTG AAGGTCAAGT GAACCTGTCT TGGTTAGGGA ATGATGCGCT GCTTCCTGCC 
CAACCCGTGC TGCAGGCAAC ACGTGCAGAA CTCTACTGCA TCACAGACTT TGCGGAAGAA 
TTAGCCGAGA TGATTGTCTC CATGGCAACC GAAATCGCAG CAATTTGCCT TGACAACTCA 
AATGGAAAAC AACCCTGGTT TTGTGCATGG AAGAGAGGGA ATGAGTTTCT GGTTACCCCC 
AACGTATCCT GCCGGTCCCT GAAGAGGAAG AAGGAAAGCC AGCCCGGCGG GGCCACCGTG 
AGGAAGCACA AGCCACCCCG GCTCAGCGAG ATCAAGAGGA AAACGGATGA GCACCCGGAG 
CTTAAGGAGA AGCTGATGAA CAGGGTCGTG GACGAGTCGA TGAACCTCGA GGACGTCCCA 
GATTCTGTCA ATATTTTTGC GAACGAAGTG GCCGCCAAGA TCATGAACCT AACGGACTTC 
TCCATGGTAG ACGGTGTCTG GCAAGCCCAG AGCCACCCCC GCACCCGGTT ACTCAGCGGA 
GACAGGTGGA GCCGACTGAA GGCCTCCAGC TGTGAAAGCA TTCCAGAGGA GGACTCTAAC 
GCCAGGGCTT ATGTAAATAG CCTGGGTTTC ATGAGTACCC TAAGTCAGCC AGTGAGCAGG 
GCCAGCTCTG TCTCCAAGCA GTCCAGCTGT GAGAGCATCA CTGAGGAGTT TTCCAGGTTC 
ATGGTGAACC AGATGGAAAA TGAAGGAAGA GGGTTTGAGT TACTGCTGGA TTACTATGCG 
GGCAAGAATG CCACCAGCAT CCTGAACTCA GCCATGCACC AGGCCTGCCA TAAAAATGAC 
CACCTCAGTG TGAGGCCGAG CTGTCCCTCG AAGCAGTCCA GCACCGAGAG CATAACCGAG 
GAGTTTTATA GGTACATGCT GAGGGACATT GAAAGAGAAA GCAGGGACAG CGCCTCCTCC 
AGACGAAGCA GCCAAGATTG GACAGTTGGC TTACTGTCAC CCTCTCTGCG ATCCCCATTG 
TGTTACAGAC AGTCATCTGT GCCCGATAGC CGATCCCTGG GTGCCAGGCT GACGGTGAAC 
CCACCCATCA AAGCCAACTC TTTAGATGGC TTCGCTCAAA ACAGCCAGCA AGATTTCCTA 
AGTGTACATC CAGTCAGTAG CACTTCCTCC TCGGGTCTCT GCAAATCTGA CTCTTGCTTG 
TATCGCAGAG GCGGAACTGA CCACATCACC AACATGTTAA TTCATGAGAC CTGGGCAAAC 
TCCATCAAGG CCCTCATGCA GAAGAACAAA ATTCTAGTGG ACGATGCCGA GGCAGCCGAT 
GCTGAGCCCG TTCCTGGTGG CTCTCCCATG CAAGTGGAGA AATGTGCAAA CAGATCGGCT 
TTGAGCAGAG TGCAAGGTGG GCCAGTTCTT CTTGTTCAGG AGTCTGTTGA TACCCCCAGG 
AAAAACTCCA TTACCGACAG CAAACATCCC CCAGTATCAT CTAAGAGCAA AGCTGCTCCT 
CTGACAAACC ACAACAGTTT AGATTCTAAG AAAGAATCTT CTTCGTGCCA TGGATCTGTG 
CCTGTCAGCC ACACCAAGCG GTCACTTTGC TCAAGGGAAG TGCCTTTAAT TCAGATTGAA 
ACAGATCAGA GAGAAGAGTG TGTTGGAGAA CCTGAACATT CCCCTTCCAA AAGCAGCCCC 
CTAGAGGAAG CAGAGGAGCA TTTGAAAGAT GCAAAAGTCC CAGAGGTGGC GAGGGGTGGA 
GACACTGCTG AGAGTGCCTG CCAAAGTCCT AGTGACAATC TTGATGCCAG CGATGTACCA 
GGGGCTGAAG TCTCGGCGGA AGGCAGAGCC CCCGATGAGT TCCCCAACCT TTCCGGCAGC 
AGTGGGGAGA GCACAGACAG CTGGTCCCAG CTCGCCAATG AGGAGGACAA TCCAGATGAC 
ACAAGTAGTT TTCTGCAGCT CAGTGAGCGA TCCATGAGCA ATGGCAACAG TAGTGCCACT 
AGCAGTCTTG GCACTATGGA CCTGGACATT TATCAGGAAA GCATGCCATC TTCTCCCATG 
ATTAATGAAT TGGTGGAAGA AAAGGAGATT CTTAAAGGAC AGTCAGAAAA CGTAGAGGAA 
GGTGCCTCTG GAGCGCCAAT GGGAGTAGCC AGCTCCCAGG GGAGCCTGCT GGTGATCAAC 
TTTGACCTGG AGCCAGAATG TCCTGATGCT GAGCTTCGAG CCACTCTGCA GTGGATAGCA 
GCCTCTGAAC TTGGGATCCC CACCATCTAC TTTAAGAAAT CTCAGGAAAA CAGAATTGAA 
AAGTTTCTAG ATGTTGTGCG ACTGGTTCAG CAGAAGTCCT GGAAAGTGGG AGATATCTTC 
CATGCGGTTG TTCAGTACTG CAAAATGCAT GAGGAGCAGA AGGAGGGGAC CCCAAGTCTC 
TTTGACTGGC TCTTGGAACT GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635852.1
CDS	271..5457
Translation	MATPGSPCPAMWVVEPKSPTNLSAALSTRRHSQESSNLESPLMYEVSAPQQGSASGSLRGSLGSSITACKKVLCSNSLLESTDYWLQNQRTPCQIGFVEDKSENCASVCFVNLDVNKDACSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVLLSGLTSGNIQADFEVSQCPWLPDICLVQCARGNRANSTNCIIFEINKFLIGLELVQERQLHLETNVLKVEDDTNCSLSSIEEDFLTASEHLEEESEVEDLRNGYENISVSANVLENKKTKEATQEEWNYNKEKLLYALEDKYIGKYHPTLMKAEGSLEKVEDTTLQSLGTSAEPLGQKGEAVWNERPAPNYYYSENVKGHVEKSQAPHTPGDAYFSRIVKNSGPPVYEHDNSLHPGDHEDGTNPLPPKQDGEATTGEYATNLAESVLQDAFIRLSQCQPTPPQESAVSVSVGNAVLPSGCSTKGMVVPRSWNELPKIVIVQSPDGSDVAPEAAITSWSDMEVSVETSGVFSGENSSRHPQSALEVALACAATVIGTISSPQATERLKMEQESLVSTHPLGGSEPLQTQISQVLKEPSISEYSFPSALCGMTQVASAIAVCGLGETGEAKAPSGLSGTAEACAVVPTPCSLATGRSMELGNEAIARALLKEAALLLTRPESCSSVGDLMESMSERIIEAASKPQTLCSENVLRNEVAQTLSSVILKHSIDEARQKSKIIDPNDTRHSPETLDTWMESTNQLLFSVLCFTFKKMSHFVQLGECPAVLVNETSNWKEPELGYWPPDQAASQAWTKASEYSSSHPLSSPRKTSLVINDLGDEVHLKPDSKGPAKPGLFKNPMLQSELPCSPRVPDSLAAKTAPKEIYLKRAAGEDPGNPHPARRRNEKEYRSPAEGERAPAGAQGCRGPQDAEDSAGADTQEKSNRVLPLNSEGQVNLSWLGNDALLPAQPVLQATRAELYCITDFAEELAEMIVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLVTPNVSCRSLKRKKESQPGGATVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNIFANEVAAKIMNLTDFSMVDGVWQAQSHPRTRLLSGDRWSRLKASSCESIPEEDSNARAYVNSLGFMSTLSQPVSRASSVSKQSSCESITEEFSRFMVNQMENEGRGFELLLDYYAGKNATSILNSAMHQACHKNDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERESRDSASSRRSSQDWTVGLLSPSLRSPLCYRQSSVPDSRSLGARLTVNPPIKANSLDGFAQNSQQDFLSVHPVSSTSSSGLCKSDSCLYRRGGTDHITNMLIHETWANSIKALMQKNKILVDDAEAADAEPVPGGSPMQVEKCANRSALSRVQGGPVLLVQESVDTPRKNSITDSKHPPVSSKSKAAPLTNHNSLDSKKESSSCHGSVPVSHTKRSLCSREVPLIQIETDQREECVGEPEHSPSKSSPLEEAEEHLKDAKVPEVARGGDTAESACQSPSDNLDASDVPGAEVSAEGRAPDEFPNLSGSSGESTDSWSQLANEEDNPDDTSSFLQLSERSMSNGNSSATSSLGTMDLDIYQESMPSSPMINELVEEKEILKGQSENVEEGASGAPMGVASSQGSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVRLVQQKSWKVGDIFHAVVQYCKMHEEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_005635795.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635795.2

ORF Insert Sequence:

ATGGCAACTC CCGGCTCTCC GTGCCCAGCC ATGTGGGTAG TTGAACCAAA GTCACCTACC 
AACCTCTCTG CTGCCTTGTC CACCAGGAGA CACTCCCAGG AATCCAGCAA TTTGGAGTCA 
CCACTGATGT ACGAGGTTTC AGCGCCACAG CAGGGCAGCG CCAGCGGCAG CTTGAGAGGC 
AGCCTGGGTA GCTCCATCAC AGCGTGTAAG AAGGTTCTTT GCAGCAACAG CCTGCTTGAG 
TCTACAGACT ACTGGTTGCA GAATCAAAGG ACACCTTGCC AGATTGGTTT TGTGGAAGAC 
AAGTCTGAAA ACTGCGCTTC TGTTTGCTTC GTGAACCTGG ATGTGAACAA GGATGCATGC 
AGCACAGAGC ACCTGCAGCA GAAATTGGTC AATGTTTCAC CAGATCTTCC AAAACTCATC 
AGCTCCATGA ACGTCCAACA GCCAAAAGAA AATGAAATTG TCCTTCTAAG TGGGTTAACA 
TCTGGAAATA TCCAGGCAGA TTTTGAAGTT TCACAGTGTC CTTGGCTGCC AGATATCTGC 
TTGGTCCAGT GTGCGAGAGG GAACAGGGCA AACAGTACCA ATTGCATCAT CTTTGAGATC 
AACAAATTTT TGATTGGTCT GGAACTGGTG CAGGAGCGGC AGCTCCACCT GGAAACAAAC 
GTCCTGAAAG TGGAGGATGA CACGAACTGT TCCCTGTCTT CCATCGAGGA GGACTTTCTC 
ACCGCCTCTG AGCACTTGGA GGAGGAAAGT GAGGTGGAAG ATTTGAGGAA CGGTTATGAA 
AACATAAGTG TCTCAGCTAA TGTTTTGGAA AATAAAAAAA CAAAGGAAGC CACTCAGGAA 
GAATGGAATT ACAACAAGGA AAAGTTACTT TATGCTTTGG AAGACAAATA TATTGGCAAA 
TATCATCCGA CGTTGATGAA AGCAGAAGGA TCTCTGGAAA AAGTAGAAGA CACCACTTTG 
CAGAGCCTGG GCACATCAGC TGAGCCATTG GGACAGAAAG GTGAAGCTGT GTGGAATGAG 
AGGCCAGCCC CAAATTATTA TTATTCGGAA AATGTTAAAG GTCACGTGGA AAAATCTCAG 
GCACCACATA CTCCCGGGGA TGCTTATTTC TCCAGGATAG TTAAGAACAG TGGGCCTCCT 
GTATATGAGC ATGACAACAG CTTACACCCA GGGGACCATG AAGATGGCAC AAACCCCCTT 
CCTCCCAAAC AAGATGGAGA AGCCACAACT GGCGAGTATG CTACAAATTT AGCAGAATCC 
GTGCTGCAAG ATGCATTTAT TCGGTTGTCC CAGTGTCAGC CTACACCTCC CCAGGAATCG 
GCAGTCAGTG TTTCTGTCGG AAATGCTGTG CTACCCAGTG GCTGTTCCAC AAAAGGTATG 
GTGGTCCCTC GGTCATGGAA TGAGCTCCCC AAAATTGTCA TTGTTCAGAG TCCAGATGGC 
AGTGACGTCG CCCCGGAGGC AGCCATCACC TCATGGTCTG ACATGGAAGT CTCTGTTGAA 
ACCTCAGGTG TCTTCTCTGG AGAGAACTCC AGCAGACACC CTCAGAGTGC TCTAGAAGTT 
GCCTTGGCCT GTGCGGCCAC TGTAATTGGA ACCATTTCCA GTCCACAGGC CACAGAAAGG 
CTCAAAATGG AGCAAGAATC TTTGGTTTCA ACGCATCCAC TGGGAGGCAG TGAACCACTG 
CAAACTCAAA TATCCCAAGT ACTCAAGGAG CCTTCCATCA GCGAATACTC CTTTCCATCT 
GCTTTGTGTG GCATGACTCA GGTGGCGAGT GCCATTGCTG TTTGTGGCCT GGGTGAAACA 
GGAGAGGCCA AGGCTCCCAG TGGCCTCTCG GGTACAGCTG AGGCCTGTGC AGTAGTTCCC 
ACACCTTGCA GTCTGGCGAC AGGGAGGAGC ATGGAGCTGG GAAATGAGGC CATTGCACGA 
GCATTGCTCA AGGAGGCCGC TCTGCTTTTA ACAAGGCCAG AATCTTGCAG CAGCGTAGGA 
GACCTCATGG AATCAATGAG CGAGAGAATC ATTGAGGCTG CTTCAAAGCC TCAGACCTTG 
TGCTCAGAAA ACGTCCTCAG GAATGAAGTA GCACAGACCC TGTCCAGTGT TATTCTGAAG 
CATTCCATTG ATGAAGCTCG CCAGAAAAGC AAAATAATTG ATCCCAACGA TACTAGGCAT 
TCTCCAGAGA CTCTTGACAC CTGGATGGAA AGTACAAATC AACTGCTCTT CAGTGTGCTT 
TGCTTCACAT TCAAGAAGAT GAGCCATTTT GTACAGCTTG GTGAGTGCCC CGCCGTCCTT 
GTTAATGAGA CCAGCAACTG GAAAGAACCA GAATTAGGCT ACTGGCCGCC TGACCAGGCT 
GCCAGTCAAG CATGGACCAA AGCCTCTGAA TATTCCAGCA GCCATCCTCT TAGCAGTCCA 
CGCAAAACTA GTCTTGTTAT CAATGATCTC GGGGATGAAG TTCATCTCAA GCCAGATAGC 
AAGGGCCCGG CGAAGCCAGG CCTGTTCAAG AACCCCATGC TGCAGTCTGA ATTGCCCTGT 
AGTCCCAGAG TGCCTGACTC TTTGGCTGCT AAAACAGCCC CCAAAGAAAT ATACCTAAAA 
AGAGCAGCGG GAGAAGATCC AGGCAACCCT CATCCTGCGC GAAGGCGCAA TGAGAAGGAG 
TACAGAAGCC CTGCAGAAGG AGAAAGGGCG CCGGCGGGGG CCCAGGGCTG CAGAGGCCCC 
CAGGATGCAG AGGACAGCGC AGGTGCCGAC ACCCAGGAGA AGTCTAACCG TGTGTTGCCC 
CTGAACAGTG AAGGTCAAGT GAACCTGTCT TGGTTAGGGA ATGATGCGCT GCTTCCTGCC 
CAACCCGTGC TGCAGGCAAC ACGTGCAGAA CTCTACTGCA TCACAGACTT TGCGGAAGAA 
TTAGCCGAGA TGATTGTCTC CATGGCAACC GAAATCGCAG CAATTTGCCT TGACAACTCA 
AATGGAAAAC AACCCTGGTT TTGTGCATGG AAGAGAGGGA ATGAGTTTCT GGTTACCCCC 
AACGTATCCT GCCGGTCCCT GAAGAGGAAG AAGGAAAGCC AGCCCGGCGG GGCCACCGTG 
AGGAAGCACA AGCCACCCCG GCTCAGCGAG ATCAAGAGGA AAACGGATGA GCACCCGGAG 
CTTAAGGAGA AGCTGATGAA CAGGGTCGTG GACGAGTCGA TGAACCTCGA GGACGTCCCA 
GATTCTGTCA ATATTTTTGC GAACGAAGTG GCCGCCAAGA TCATGAACCT AACGGACTTC 
TCCATGGTAG ACGGTGTCTG GCAAGCCCAG AGCCACCCCC GCACCCGGTT ACTCAGCGGA 
GACAGGTGGA GCCGACTGAA GGCCTCCAGC TGTGAAAGCA TTCCAGAGGA GGACTCTAAC 
GCCAGGGCTT ATGTAAATAG CCTGGGTTTC ATGAGTACCC TAAGTCAGCC AGTGAGCAGG 
GCCAGCTCTG TCTCCAAGCA GTCCAGCTGT GAGAGCATCA CTGAGGAGTT TTCCAGGTTC 
ATGGTGAACC AGATGGAAAA TGAAGGAAGA GGGTTTGAGT TACTGCTGGA TTACTATGCG 
GGCAAGAATG CCACCAGCAT CCTGAACTCA GCCATGCACC AGGCCTGCCA TAAAAATGAC 
CACCTCAGTG TGAGGCCGAG CTGTCCCTCG AAGCAGTCCA GCACCGAGAG CATAACCGAG 
GAGTTTTATA GGTACATGCT GAGGGACATT GAAAGAGAAA GCAGGGACAG CGCCTCCTCC 
AGACGAAGCA GCCAAGATTG GACAGTTGGC TTACTGTCAC CCTCTCTGCG ATCCCCATTG 
TGTTACAGAC AGTCATCTGT GCCCGATAGC CGATCCCTGG GTGCCAGGCT GACGGTGAAC 
CCACCCATCA AAGCCAACTC TTTAGATGGC TTCGCTCAAA ACAGCCAGCA AGATTTCCTA 
AGTGTACATC CAGTCAGTAG CACTTCCTCC TCGGGTCTCT GCAAATCTGA CTCTTGCTTG 
TATCGCAGAG GCGGAACTGA CCACATCACC AACATGTTAA TTCATGAGAC CTGGGCAAAC 
TCCATCAAGG CCCTCATGCA GAAGAACAAA ATTCTAGTGG ACGATGCCGA GGCAGCCGAT 
GCTGAGCCCG TTCCTGGTGG CTCTCCCATG CAAGTGGAGA AATGTGCAAA CAGATCGGCT 
TTGAGCAGAG TGCAAGGTGG GCCAGTTCTT CTTGTTCAGG AGTCTGTTGA TACCCCCAGG 
AAAAACTCCA TTACCGACAG CAAACATCCC CCAGTATCAT CTAAGAGCAA AGCTGCTCCT 
CTGACAAACC ACAACAGTTT AGATTCTAAG AAAGAATCTT CTTCGTGCCA TGGATCTGTG 
CCTGTCAGCC ACACCAAGCG GTCACTTTGC TCAAGGGAAG TGCCTTTAAT TCAGATTGAA 
ACAGATCAGA GAGAAGAGTG TGTTGGAGAA CCTGAACATT CCCCTTCCAA AAGCAGCCCC 
CTAGAGGAAG CAGAGGAGCA TTTGAAAGAT GCAAAAGTCC CAGAGGTGGC GAGGGGTGGA 
GACACTGCTG AGAGTGCCTG CCAAAGTCCT AGTGACAATC TTGATGCCAG CGATGTACCA 
GGGGCTGAAG TCTCGGCGGA AGGCAGAGCC CCCGATGAGT TCCCCAACCT TTCCGGCAGC 
AGTGGGGAGA GCACAGACAG CTGGTCCCAG CTCGCCAATG AGGAGGACAA TCCAGATGAC 
ACAAGTAGTT TTCTGCAGCT CAGTGAGCGA TCCATGAGCA ATGGCAACAG TAGTGCCACT 
AGCAGTCTTG GCACTATGGA CCTGGACATT TATCAGGAAA GCATGCCATC TTCTCCCATG 
ATTAATGAAT TGGTGGAAGA AAAGGAGATT CTTAAAGGAC AGTCAGAAAA CGTAGAGGAA 
GGTGCCTCTG GAGCGCCAAT GGGAGTAGCC AGCTCCCAGG GGAGCCTGCT GGTGATCAAC 
TTTGACCTGG AGCCAGAATG TCCTGATGCT GAGCTTCGAG CCACTCTGCA GTGGATAGCA 
GCCTCTGAAC TTGGGATCCC CACCATCTAC TTTAAGAAAT CTCAGGAAAA CAGAATTGAA 
AAGTTTCTAG ATGTTGTGCG ACTGGTTCAG CAGAAGTCCT GGAAAGTGGG AGATATCTTC 
CATGCGGTTG TTCAGTACTG CAAAATGCAT GAGGAGCAGA AGGAGGGGAC CCCAAGTCTC 
TTTGACTGGC TCTTGGAACT GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_005635796.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635853.1 Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe25757
Clone ID Related Accession (Same CDS sequence)	XM_005635796.1 , XM_005635796.2
Accession Version	XM_005635796.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CCGGCTCTCC GTGCCCAGCC ATGTGGGTAG TTGAACCAAA GTCACCTACC 
AACCTCTCTG CTGCCTTGTC CACCAGGAGA CACTCCCAGG AATCCAGCAA TTTGGAGTCA 
CCACTGATGT ACGAGGTTTC AGCGCCACAG CAGGGCAGCG CCAGCGGCAG CTTGAGAGGC 
AGCCTGGGTA GCTCCATCAC AGCGTGTAAG AAGGTTCTTT GCAGCAACAG CCTGCTTGAG 
TCTACAGACT ACTGGTTGCA GAATCAAAGG ACACCTTGCC AGATTGGTTT TGTGGAAGAC 
AAGTCTGAAA ACTGCGCTTC TGTTTGCTTC GTGAACCTGG ATGTGAACAA GGATGCATGC 
AGCACAGAGC ACCTGCAGCA GAAATTGGTC AATGTTTCAC CAGATCTTCC AAAACTCATC 
AGCTCCATGA ACGTCCAACA GCCAAAAGAA AATGAAATTG TCCTTCTAAG TGGGTTAACA 
TCTGGAAATA TCCAGGCAGA TTTTGAAGTT TCACAGTGTC CTTGGCTGCC AGATATCTGC 
TTGGTCCAGT GTGCGAGAGG GAACAGGGCA AACAGTACCA ATTGCATCAT CTTTGAGATC 
AACAAATTTT TGATTGGTCT GGAACTGGTG CAGGAGCGGC AGCTCCACCT GGAAACAAAC 
GTCCTGAAAG TGGAGGATGA CACGAACTGT TCCCTGTCTT CCATCGAGGA GGACTTTCTC 
ACCGCCTCTG AGCACTTGGA GGAGGAAAGT GAGGTGGAAG ATTTGAGGAA CGGTTATGAA 
AACATAAGTG TCTCAGCTAA TGTTTTGGAA AATAAAAAAA CAAAGGAAGC CACTCAGGAA 
GAATGGAATT ACAACAAGGA AAAGTTACTT TATGCTTTGG AAGACAAATA TATTGGCAAA 
TATCATCCGA CGTTGATGAA AGCAGAAGGA TCTCTGGAAA AAGTAGAAGA CACCACTTTG 
CAGAGCCTGG GCACATCAGC TGAGCCATTG GGACAGAAAG GTGAAGCTGT GTGGAATGAG 
AGGCCAGCCC CAAATTATTA TTATTCGGAA AATGTTAAAG GTCACGTGGA AAAATCTCAG 
GCACCACATA CTCCCGGGGA TGCTTATTTC TCCAGGATAG TTAAGAACAG TGGGCCTCCT 
GTATATGAGC ATGACAACAG CTTACACCCA GGGGACCATG AAGATGGCAC AAACCCCCTT 
CCTCCCAAAC AAGATGGAGA AGCCACAACT GGCGAGTATG CTACAAATTT AGCAGAATCC 
GTGCTGCAAG ATGCATTTAT TCGGTTGTCC CAGTGTCAGC CTACACCTCC CCAGGAATCG 
GCAGTCAGTG TTTCTGTCGG AAATGCTGTG CTACCCAGTG GCTGTTCCAC AAAAGGTATG 
GTGGTCCCTC GGTCATGGAA TGAGCTCCCC AAAATTGTCA TTGTTCAGAG TCCAGATGGC 
AGTGACGTCG CCCCGGAGGC AGCCATCACC TCATGGTCTG ACATGGAAGT CTCTGTTGAA 
ACCTCAGGTG TCTTCTCTGG AGAGAACTCC AGCAGACACC CTCAGAGTGC TCTAGAAGTT 
GCCTTGGCCT GTGCGGCCAC TGTAATTGGA ACCATTTCCA GTCCACAGGC CACAGAAAGG 
CTCAAAATGG AGCAAGAATC TTTGGTTTCA ACGCATCCAC TGGGAGGCAG TGAACCACTG 
CAAACTCAAA TATCCCAAGT ACTCAAGGAG CCTTCCATCA GCGAATACTC CTTTCCATCT 
GCTTTGTGTG GCATGACTCA GGTGGCGAGT GCCATTGCTG TTTGTGGCCT GGGTGAAACA 
GGAGAGGCCA AGGCTCCCAG TGGCCTCTCG GGTACAGCTG AGGCCTGTGC AGTAGTTCCC 
ACACCTTGCA GTCTGGCGAC AGGGAGGAGC ATGGAGCTGG GAAATGAGGC CATTGCACGA 
GCATTGCTCA AGGAGGCCGC TCTGCTTTTA ACAAGGCCAG AATCTTGCAG CAGCGTAGGA 
GACCTCATGG AATCAATGAG CGAGAGAATC ATTGAGGCTG CTTCAAAGCC TCAGACCTTG 
TGCTCAGAAA ACGTCCTCAG GAATGAAGTA GCACAGACCC TGTCCAGTGT TATTCTGAAG 
CATTCCATTG ATGAAGCTCG CCAGAAAAGC AAAATAATTG ATCCCAACGA TACTAGGCAT 
TCTCCAGAGA CTCTTGACAC CTGGATGGAA AGTACAAATC AACTGCTCTT CAGTGTGCTT 
TGCTTCACAT TCAAGAAGAT GAGCCATTTT GTACAGCTTG GTGAGTGCCC CGCCGTCCTT 
GTTAATGAGA CCAGCAACTG GAAAGAACCA GAATTAGGCT ACTGGCCGCC TGACCAGGCT 
GCCAGTCAAG CATGGACCAA AGCCTCTGAA TATTCCAGCA GCCATCCTCT TAGCAGTCCA 
CGCAAAACTA GTCTTGTTAT CAATGATCTC GGGGATGAAG TTCATCTCAA GCCAGATAGC 
AAGGGCCCGG CGAAGCCAGG CCTGTTCAAG AACCCCATGC TGCAGTCTGA ATTGCCCTGT 
AGTCCCAGAG TGCCTGACTC TTTGGCTGCT AAAACAGCCC CCAAAGAAAT ATACCTAAAA 
AGAGCAGCGG GAGAAGATCC AGGCAACCCT CATCCTGCGC GAAGGCGCAA TGAGAAGGAG 
TACAGAAGCC CTGCAGAAGG AGAAAGGGCG CCGGCGGGGG CCCAGGGCTG CAGAGGCCCC 
CAGGATGCAG AGGACAGCGC AGGTGCCGAC ACCCAGGAGA AGTCTAACCG TGTGTTGCCC 
CTGAACAGTG AAGGTCAAGT GAACCTGTCT TGGTTAGGGA ATGATGCGCT GCTTCCTGCC 
CAACCCGTGC TGCAGGCAAC ACGTGCAGAA CTCTACTGCA TCACAGACTT TGCGGAAGAA 
TTAGCCGAGA TGATTGTCTC CATGGCAACC GAAATCGCAG CAATTTGCCT TGACAACTCA 
AATGGAAAAC AACCCTGGTT TTGTGCATGG AAGAGAGGGA ATGAGTTTCT GGTTACCCCC 
AACGTATCCT GCCGGTCCCT GAAGAGGAAG AAGGAAAGCC AGCCCGGCGG GGCCACCGTG 
AGGAAGCACA AGCCACCCCG GCTCAGCGAG ATCAAGAGGA AAACGGATGA GCACCCGGAG 
CTTAAGGAGA AGCTGATGAA CAGGGTCGTG GACGAGTCGA TGAACCTCGA GGACGTCCCA 
GATTCTGTCA ATATTTTTGC GAACGAAGTG GCCGCCAAGA TCATGAACCT AACGGACTTC 
TCCATGGTAG ACGGTGTCTG GCAAGCCCAG AGCCACCCCC GCACCCGGTT ACTCAGCGGA 
GACAGGTGGA GCCGACTGAA GGCCTCCAGC TGTGAAAGCA TTCCAGAGGA GGACTCTAAC 
GCCAGGGCTT ATGTAAATAG CCTGGGTTTC ATGAGTACCC TAAGTCAGCC AGTGAGCAGG 
GCCAGCTCTG TCTCCAAGCA GTCCAGCTGT GAGAGCATCA CTGAGGAGTT TTCCAGGTTC 
ATGGTGAACC AGATGGAAAA TGAAGGAAGA GGGTTTGAGT TACTGCTGGA TTACTATGCG 
GGCAAGAATG CCACCAGCAT CCTGAACTCA GCCATGCACC AGGCCTGCCA TAAAAATGAC 
CACCTCAGTG TGAGGCCGAG CTGTCCCTCG AAGCAGTCCA GCACCGAGAG CATAACCGAG 
GAGTTTTATA GGTACATGCT GAGGGACATT GAAAGAGAAA GCAGGGACAG CGCCTCCTCC 
AGACGAAGCA GCCAAGATTG GACAGTTGGC TTACTGTCAC CCTCTCTGCG ATCCCCATTG 
TGTTACAGAC AGTCATCTGT GCCCGATAGC CGATCCCTGG GTGCCAGGCT GACGGTGAAC 
CCACCCATCA AAGCCAACTC TTTAGATGGC TTCGCTCAAA ACAGCCAGCA AGATTTCCTA 
AGTGTACATC CAGTCAGTAG CACTTCCTCC TCGGGTCTCT GCAAATCTGA CTCTTGCTTG 
TATCGCAGAG GCGGAACTGA CCACATCACC AACATGTTAA TTCATGAGAC CTGGGCAAAC 
TCCATCAAGG CCCTCATGCA GAAGAACAAA ATTCTAGTGG ACGATGCCGA GGCAGCCGAT 
GCTGAGCCCG TTCCTGGTGG CTCTCCCATG CAAGTGGAGA AATGTGCAAA CAGATCGGCT 
TTGAGCAGAG TGCAAGGTGG GCCAGTTCTT CTTGTTCAGG AGTCTGTTGA TACCCCCAGG 
AAAAACTCCA TTACCGACAG CAAACATCCC CCAGTATCAT CTAAGAGCAA AGCTGCTCCT 
CTGACAAACC ACAACAGTTT AGATTCTAAG AAAGAATCTT CTTCGTGCCA TGGATCTGTG 
CCTGTCAGCC ACACCAAGCG GTCACTTTGC TCAAGGGAAG TGCCTTTAAT TCAGATTGAA 
ACAGATCAGA GAGAAGAGTG TGTTGGAGAA CCTGAACATT CCCCTTCCAA AAGCAGCCCC 
CTAGAGGAAG CAGAGGAGCA TTTGAAAGAT GCAAAAGTCC CAGAGGTGGC GAGGGGTGGA 
GACACTGCTG AGAGTGCCTG CCAAAGTCCT AGTGACAATC TTGATGCCAG CGATGTACCA 
GGGGCTGAAG TCTCGGCGGA AGGCAGAGCC CCCGATGAGT TCCCCAACCT TTCCGGCAGC 
AGTGGGGAGA GCACAGACAG CTGGTCCCAG CTCGCCAATG AGGAGGACAA TCCAGATGAC 
ACAAGTAGTT TTCTGCAGCT CAGTGAGCGA TCCATGAGTG AATTGGTGGA AGAAAAGGAG 
ATTCTTAAAG GACAGTCAGA AAACGTAGAG GAAGGTGCCT CTGGAGCGCC AATGGGAGTA 
GCCAGCTCCC AGGGGAGCCT GCTGGTGATC AACTTTGACC TGGAGCCAGA ATGTCCTGAT 
GCTGAGCTTC GAGCCACTCT GCAGTGGATA GCAGCCTCTG AACTTGGGAT CCCCACCATC 
TACTTTAAGA AATCTCAGGA AAACAGAATT GAAAAGTTTC TAGATGTTGT GCGACTGGTT 
CAGCAGAAGT CCTGGAAAGT GGGAGATATC TTCCATGCGG TTGTTCAGTA CTGCAAAATG 
CATGAGGAGC AGAAGGAGGG GACCCCAAGT CTCTTTGACT GGCTCTTGGA ACTGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635853.1
CDS	308..5407
Translation	MATPGSPCPAMWVVEPKSPTNLSAALSTRRHSQESSNLESPLMYEVSAPQQGSASGSLRGSLGSSITACKKVLCSNSLLESTDYWLQNQRTPCQIGFVEDKSENCASVCFVNLDVNKDACSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVLLSGLTSGNIQADFEVSQCPWLPDICLVQCARGNRANSTNCIIFEINKFLIGLELVQERQLHLETNVLKVEDDTNCSLSSIEEDFLTASEHLEEESEVEDLRNGYENISVSANVLENKKTKEATQEEWNYNKEKLLYALEDKYIGKYHPTLMKAEGSLEKVEDTTLQSLGTSAEPLGQKGEAVWNERPAPNYYYSENVKGHVEKSQAPHTPGDAYFSRIVKNSGPPVYEHDNSLHPGDHEDGTNPLPPKQDGEATTGEYATNLAESVLQDAFIRLSQCQPTPPQESAVSVSVGNAVLPSGCSTKGMVVPRSWNELPKIVIVQSPDGSDVAPEAAITSWSDMEVSVETSGVFSGENSSRHPQSALEVALACAATVIGTISSPQATERLKMEQESLVSTHPLGGSEPLQTQISQVLKEPSISEYSFPSALCGMTQVASAIAVCGLGETGEAKAPSGLSGTAEACAVVPTPCSLATGRSMELGNEAIARALLKEAALLLTRPESCSSVGDLMESMSERIIEAASKPQTLCSENVLRNEVAQTLSSVILKHSIDEARQKSKIIDPNDTRHSPETLDTWMESTNQLLFSVLCFTFKKMSHFVQLGECPAVLVNETSNWKEPELGYWPPDQAASQAWTKASEYSSSHPLSSPRKTSLVINDLGDEVHLKPDSKGPAKPGLFKNPMLQSELPCSPRVPDSLAAKTAPKEIYLKRAAGEDPGNPHPARRRNEKEYRSPAEGERAPAGAQGCRGPQDAEDSAGADTQEKSNRVLPLNSEGQVNLSWLGNDALLPAQPVLQATRAELYCITDFAEELAEMIVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLVTPNVSCRSLKRKKESQPGGATVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNIFANEVAAKIMNLTDFSMVDGVWQAQSHPRTRLLSGDRWSRLKASSCESIPEEDSNARAYVNSLGFMSTLSQPVSRASSVSKQSSCESITEEFSRFMVNQMENEGRGFELLLDYYAGKNATSILNSAMHQACHKNDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERESRDSASSRRSSQDWTVGLLSPSLRSPLCYRQSSVPDSRSLGARLTVNPPIKANSLDGFAQNSQQDFLSVHPVSSTSSSGLCKSDSCLYRRGGTDHITNMLIHETWANSIKALMQKNKILVDDAEAADAEPVPGGSPMQVEKCANRSALSRVQGGPVLLVQESVDTPRKNSITDSKHPPVSSKSKAAPLTNHNSLDSKKESSSCHGSVPVSHTKRSLCSREVPLIQIETDQREECVGEPEHSPSKSSPLEEAEEHLKDAKVPEVARGGDTAESACQSPSDNLDASDVPGAEVSAEGRAPDEFPNLSGSSGESTDSWSQLANEEDNPDDTSSFLQLSERSMSELVEEKEILKGQSENVEEGASGAPMGVASSQGSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVRLVQQKSWKVGDIFHAVVQYCKMHEEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_005635796.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635796.1

ORF Insert Sequence:

ATGGCAACTC CCGGCTCTCC GTGCCCAGCC ATGTGGGTAG TTGAACCAAA GTCACCTACC 
AACCTCTCTG CTGCCTTGTC CACCAGGAGA CACTCCCAGG AATCCAGCAA TTTGGAGTCA 
CCACTGATGT ACGAGGTTTC AGCGCCACAG CAGGGCAGCG CCAGCGGCAG CTTGAGAGGC 
AGCCTGGGTA GCTCCATCAC AGCGTGTAAG AAGGTTCTTT GCAGCAACAG CCTGCTTGAG 
TCTACAGACT ACTGGTTGCA GAATCAAAGG ACACCTTGCC AGATTGGTTT TGTGGAAGAC 
AAGTCTGAAA ACTGCGCTTC TGTTTGCTTC GTGAACCTGG ATGTGAACAA GGATGCATGC 
AGCACAGAGC ACCTGCAGCA GAAATTGGTC AATGTTTCAC CAGATCTTCC AAAACTCATC 
AGCTCCATGA ACGTCCAACA GCCAAAAGAA AATGAAATTG TCCTTCTAAG TGGGTTAACA 
TCTGGAAATA TCCAGGCAGA TTTTGAAGTT TCACAGTGTC CTTGGCTGCC AGATATCTGC 
TTGGTCCAGT GTGCGAGAGG GAACAGGGCA AACAGTACCA ATTGCATCAT CTTTGAGATC 
AACAAATTTT TGATTGGTCT GGAACTGGTG CAGGAGCGGC AGCTCCACCT GGAAACAAAC 
GTCCTGAAAG TGGAGGATGA CACGAACTGT TCCCTGTCTT CCATCGAGGA GGACTTTCTC 
ACCGCCTCTG AGCACTTGGA GGAGGAAAGT GAGGTGGAAG ATTTGAGGAA CGGTTATGAA 
AACATAAGTG TCTCAGCTAA TGTTTTGGAA AATAAAAAAA CAAAGGAAGC CACTCAGGAA 
GAATGGAATT ACAACAAGGA AAAGTTACTT TATGCTTTGG AAGACAAATA TATTGGCAAA 
TATCATCCGA CGTTGATGAA AGCAGAAGGA TCTCTGGAAA AAGTAGAAGA CACCACTTTG 
CAGAGCCTGG GCACATCAGC TGAGCCATTG GGACAGAAAG GTGAAGCTGT GTGGAATGAG 
AGGCCAGCCC CAAATTATTA TTATTCGGAA AATGTTAAAG GTCACGTGGA AAAATCTCAG 
GCACCACATA CTCCCGGGGA TGCTTATTTC TCCAGGATAG TTAAGAACAG TGGGCCTCCT 
GTATATGAGC ATGACAACAG CTTACACCCA GGGGACCATG AAGATGGCAC AAACCCCCTT 
CCTCCCAAAC AAGATGGAGA AGCCACAACT GGCGAGTATG CTACAAATTT AGCAGAATCC 
GTGCTGCAAG ATGCATTTAT TCGGTTGTCC CAGTGTCAGC CTACACCTCC CCAGGAATCG 
GCAGTCAGTG TTTCTGTCGG AAATGCTGTG CTACCCAGTG GCTGTTCCAC AAAAGGTATG 
GTGGTCCCTC GGTCATGGAA TGAGCTCCCC AAAATTGTCA TTGTTCAGAG TCCAGATGGC 
AGTGACGTCG CCCCGGAGGC AGCCATCACC TCATGGTCTG ACATGGAAGT CTCTGTTGAA 
ACCTCAGGTG TCTTCTCTGG AGAGAACTCC AGCAGACACC CTCAGAGTGC TCTAGAAGTT 
GCCTTGGCCT GTGCGGCCAC TGTAATTGGA ACCATTTCCA GTCCACAGGC CACAGAAAGG 
CTCAAAATGG AGCAAGAATC TTTGGTTTCA ACGCATCCAC TGGGAGGCAG TGAACCACTG 
CAAACTCAAA TATCCCAAGT ACTCAAGGAG CCTTCCATCA GCGAATACTC CTTTCCATCT 
GCTTTGTGTG GCATGACTCA GGTGGCGAGT GCCATTGCTG TTTGTGGCCT GGGTGAAACA 
GGAGAGGCCA AGGCTCCCAG TGGCCTCTCG GGTACAGCTG AGGCCTGTGC AGTAGTTCCC 
ACACCTTGCA GTCTGGCGAC AGGGAGGAGC ATGGAGCTGG GAAATGAGGC CATTGCACGA 
GCATTGCTCA AGGAGGCCGC TCTGCTTTTA ACAAGGCCAG AATCTTGCAG CAGCGTAGGA 
GACCTCATGG AATCAATGAG CGAGAGAATC ATTGAGGCTG CTTCAAAGCC TCAGACCTTG 
TGCTCAGAAA ACGTCCTCAG GAATGAAGTA GCACAGACCC TGTCCAGTGT TATTCTGAAG 
CATTCCATTG ATGAAGCTCG CCAGAAAAGC AAAATAATTG ATCCCAACGA TACTAGGCAT 
TCTCCAGAGA CTCTTGACAC CTGGATGGAA AGTACAAATC AACTGCTCTT CAGTGTGCTT 
TGCTTCACAT TCAAGAAGAT GAGCCATTTT GTACAGCTTG GTGAGTGCCC CGCCGTCCTT 
GTTAATGAGA CCAGCAACTG GAAAGAACCA GAATTAGGCT ACTGGCCGCC TGACCAGGCT 
GCCAGTCAAG CATGGACCAA AGCCTCTGAA TATTCCAGCA GCCATCCTCT TAGCAGTCCA 
CGCAAAACTA GTCTTGTTAT CAATGATCTC GGGGATGAAG TTCATCTCAA GCCAGATAGC 
AAGGGCCCGG CGAAGCCAGG CCTGTTCAAG AACCCCATGC TGCAGTCTGA ATTGCCCTGT 
AGTCCCAGAG TGCCTGACTC TTTGGCTGCT AAAACAGCCC CCAAAGAAAT ATACCTAAAA 
AGAGCAGCGG GAGAAGATCC AGGCAACCCT CATCCTGCGC GAAGGCGCAA TGAGAAGGAG 
TACAGAAGCC CTGCAGAAGG AGAAAGGGCG CCGGCGGGGG CCCAGGGCTG CAGAGGCCCC 
CAGGATGCAG AGGACAGCGC AGGTGCCGAC ACCCAGGAGA AGTCTAACCG TGTGTTGCCC 
CTGAACAGTG AAGGTCAAGT GAACCTGTCT TGGTTAGGGA ATGATGCGCT GCTTCCTGCC 
CAACCCGTGC TGCAGGCAAC ACGTGCAGAA CTCTACTGCA TCACAGACTT TGCGGAAGAA 
TTAGCCGAGA TGATTGTCTC CATGGCAACC GAAATCGCAG CAATTTGCCT TGACAACTCA 
AATGGAAAAC AACCCTGGTT TTGTGCATGG AAGAGAGGGA ATGAGTTTCT GGTTACCCCC 
AACGTATCCT GCCGGTCCCT GAAGAGGAAG AAGGAAAGCC AGCCCGGCGG GGCCACCGTG 
AGGAAGCACA AGCCACCCCG GCTCAGCGAG ATCAAGAGGA AAACGGATGA GCACCCGGAG 
CTTAAGGAGA AGCTGATGAA CAGGGTCGTG GACGAGTCGA TGAACCTCGA GGACGTCCCA 
GATTCTGTCA ATATTTTTGC GAACGAAGTG GCCGCCAAGA TCATGAACCT AACGGACTTC 
TCCATGGTAG ACGGTGTCTG GCAAGCCCAG AGCCACCCCC GCACCCGGTT ACTCAGCGGA 
GACAGGTGGA GCCGACTGAA GGCCTCCAGC TGTGAAAGCA TTCCAGAGGA GGACTCTAAC 
GCCAGGGCTT ATGTAAATAG CCTGGGTTTC ATGAGTACCC TAAGTCAGCC AGTGAGCAGG 
GCCAGCTCTG TCTCCAAGCA GTCCAGCTGT GAGAGCATCA CTGAGGAGTT TTCCAGGTTC 
ATGGTGAACC AGATGGAAAA TGAAGGAAGA GGGTTTGAGT TACTGCTGGA TTACTATGCG 
GGCAAGAATG CCACCAGCAT CCTGAACTCA GCCATGCACC AGGCCTGCCA TAAAAATGAC 
CACCTCAGTG TGAGGCCGAG CTGTCCCTCG AAGCAGTCCA GCACCGAGAG CATAACCGAG 
GAGTTTTATA GGTACATGCT GAGGGACATT GAAAGAGAAA GCAGGGACAG CGCCTCCTCC 
AGACGAAGCA GCCAAGATTG GACAGTTGGC TTACTGTCAC CCTCTCTGCG ATCCCCATTG 
TGTTACAGAC AGTCATCTGT GCCCGATAGC CGATCCCTGG GTGCCAGGCT GACGGTGAAC 
CCACCCATCA AAGCCAACTC TTTAGATGGC TTCGCTCAAA ACAGCCAGCA AGATTTCCTA 
AGTGTACATC CAGTCAGTAG CACTTCCTCC TCGGGTCTCT GCAAATCTGA CTCTTGCTTG 
TATCGCAGAG GCGGAACTGA CCACATCACC AACATGTTAA TTCATGAGAC CTGGGCAAAC 
TCCATCAAGG CCCTCATGCA GAAGAACAAA ATTCTAGTGG ACGATGCCGA GGCAGCCGAT 
GCTGAGCCCG TTCCTGGTGG CTCTCCCATG CAAGTGGAGA AATGTGCAAA CAGATCGGCT 
TTGAGCAGAG TGCAAGGTGG GCCAGTTCTT CTTGTTCAGG AGTCTGTTGA TACCCCCAGG 
AAAAACTCCA TTACCGACAG CAAACATCCC CCAGTATCAT CTAAGAGCAA AGCTGCTCCT 
CTGACAAACC ACAACAGTTT AGATTCTAAG AAAGAATCTT CTTCGTGCCA TGGATCTGTG 
CCTGTCAGCC ACACCAAGCG GTCACTTTGC TCAAGGGAAG TGCCTTTAAT TCAGATTGAA 
ACAGATCAGA GAGAAGAGTG TGTTGGAGAA CCTGAACATT CCCCTTCCAA AAGCAGCCCC 
CTAGAGGAAG CAGAGGAGCA TTTGAAAGAT GCAAAAGTCC CAGAGGTGGC GAGGGGTGGA 
GACACTGCTG AGAGTGCCTG CCAAAGTCCT AGTGACAATC TTGATGCCAG CGATGTACCA 
GGGGCTGAAG TCTCGGCGGA AGGCAGAGCC CCCGATGAGT TCCCCAACCT TTCCGGCAGC 
AGTGGGGAGA GCACAGACAG CTGGTCCCAG CTCGCCAATG AGGAGGACAA TCCAGATGAC 
ACAAGTAGTT TTCTGCAGCT CAGTGAGCGA TCCATGAGTG AATTGGTGGA AGAAAAGGAG 
ATTCTTAAAG GACAGTCAGA AAACGTAGAG GAAGGTGCCT CTGGAGCGCC AATGGGAGTA 
GCCAGCTCCC AGGGGAGCCT GCTGGTGATC AACTTTGACC TGGAGCCAGA ATGTCCTGAT 
GCTGAGCTTC GAGCCACTCT GCAGTGGATA GCAGCCTCTG AACTTGGGAT CCCCACCATC 
TACTTTAAGA AATCTCAGGA AAACAGAATT GAAAAGTTTC TAGATGTTGT GCGACTGGTT 
CAGCAGAAGT CCTGGAAAGT GGGAGATATC TTCCATGCGG TTGTTCAGTA CTGCAAAATG 
CATGAGGAGC AGAAGGAGGG GACCCCAAGT CTCTTTGACT GGCTCTTGGA ACTGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_005635796.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635853.1 Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

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CloneID	OCe25757
Clone ID Related Accession (Same CDS sequence)	XM_005635796.1 , XM_005635796.2
Accession Version	XM_005635796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CCGGCTCTCC GTGCCCAGCC ATGTGGGTAG TTGAACCAAA GTCACCTACC 
AACCTCTCTG CTGCCTTGTC CACCAGGAGA CACTCCCAGG AATCCAGCAA TTTGGAGTCA 
CCACTGATGT ACGAGGTTTC AGCGCCACAG CAGGGCAGCG CCAGCGGCAG CTTGAGAGGC 
AGCCTGGGTA GCTCCATCAC AGCGTGTAAG AAGGTTCTTT GCAGCAACAG CCTGCTTGAG 
TCTACAGACT ACTGGTTGCA GAATCAAAGG ACACCTTGCC AGATTGGTTT TGTGGAAGAC 
AAGTCTGAAA ACTGCGCTTC TGTTTGCTTC GTGAACCTGG ATGTGAACAA GGATGCATGC 
AGCACAGAGC ACCTGCAGCA GAAATTGGTC AATGTTTCAC CAGATCTTCC AAAACTCATC 
AGCTCCATGA ACGTCCAACA GCCAAAAGAA AATGAAATTG TCCTTCTAAG TGGGTTAACA 
TCTGGAAATA TCCAGGCAGA TTTTGAAGTT TCACAGTGTC CTTGGCTGCC AGATATCTGC 
TTGGTCCAGT GTGCGAGAGG GAACAGGGCA AACAGTACCA ATTGCATCAT CTTTGAGATC 
AACAAATTTT TGATTGGTCT GGAACTGGTG CAGGAGCGGC AGCTCCACCT GGAAACAAAC 
GTCCTGAAAG TGGAGGATGA CACGAACTGT TCCCTGTCTT CCATCGAGGA GGACTTTCTC 
ACCGCCTCTG AGCACTTGGA GGAGGAAAGT GAGGTGGAAG ATTTGAGGAA CGGTTATGAA 
AACATAAGTG TCTCAGCTAA TGTTTTGGAA AATAAAAAAA CAAAGGAAGC CACTCAGGAA 
GAATGGAATT ACAACAAGGA AAAGTTACTT TATGCTTTGG AAGACAAATA TATTGGCAAA 
TATCATCCGA CGTTGATGAA AGCAGAAGGA TCTCTGGAAA AAGTAGAAGA CACCACTTTG 
CAGAGCCTGG GCACATCAGC TGAGCCATTG GGACAGAAAG GTGAAGCTGT GTGGAATGAG 
AGGCCAGCCC CAAATTATTA TTATTCGGAA AATGTTAAAG GTCACGTGGA AAAATCTCAG 
GCACCACATA CTCCCGGGGA TGCTTATTTC TCCAGGATAG TTAAGAACAG TGGGCCTCCT 
GTATATGAGC ATGACAACAG CTTACACCCA GGGGACCATG AAGATGGCAC AAACCCCCTT 
CCTCCCAAAC AAGATGGAGA AGCCACAACT GGCGAGTATG CTACAAATTT AGCAGAATCC 
GTGCTGCAAG ATGCATTTAT TCGGTTGTCC CAGTGTCAGC CTACACCTCC CCAGGAATCG 
GCAGTCAGTG TTTCTGTCGG AAATGCTGTG CTACCCAGTG GCTGTTCCAC AAAAGGTATG 
GTGGTCCCTC GGTCATGGAA TGAGCTCCCC AAAATTGTCA TTGTTCAGAG TCCAGATGGC 
AGTGACGTCG CCCCGGAGGC AGCCATCACC TCATGGTCTG ACATGGAAGT CTCTGTTGAA 
ACCTCAGGTG TCTTCTCTGG AGAGAACTCC AGCAGACACC CTCAGAGTGC TCTAGAAGTT 
GCCTTGGCCT GTGCGGCCAC TGTAATTGGA ACCATTTCCA GTCCACAGGC CACAGAAAGG 
CTCAAAATGG AGCAAGAATC TTTGGTTTCA ACGCATCCAC TGGGAGGCAG TGAACCACTG 
CAAACTCAAA TATCCCAAGT ACTCAAGGAG CCTTCCATCA GCGAATACTC CTTTCCATCT 
GCTTTGTGTG GCATGACTCA GGTGGCGAGT GCCATTGCTG TTTGTGGCCT GGGTGAAACA 
GGAGAGGCCA AGGCTCCCAG TGGCCTCTCG GGTACAGCTG AGGCCTGTGC AGTAGTTCCC 
ACACCTTGCA GTCTGGCGAC AGGGAGGAGC ATGGAGCTGG GAAATGAGGC CATTGCACGA 
GCATTGCTCA AGGAGGCCGC TCTGCTTTTA ACAAGGCCAG AATCTTGCAG CAGCGTAGGA 
GACCTCATGG AATCAATGAG CGAGAGAATC ATTGAGGCTG CTTCAAAGCC TCAGACCTTG 
TGCTCAGAAA ACGTCCTCAG GAATGAAGTA GCACAGACCC TGTCCAGTGT TATTCTGAAG 
CATTCCATTG ATGAAGCTCG CCAGAAAAGC AAAATAATTG ATCCCAACGA TACTAGGCAT 
TCTCCAGAGA CTCTTGACAC CTGGATGGAA AGTACAAATC AACTGCTCTT CAGTGTGCTT 
TGCTTCACAT TCAAGAAGAT GAGCCATTTT GTACAGCTTG GTGAGTGCCC CGCCGTCCTT 
GTTAATGAGA CCAGCAACTG GAAAGAACCA GAATTAGGCT ACTGGCCGCC TGACCAGGCT 
GCCAGTCAAG CATGGACCAA AGCCTCTGAA TATTCCAGCA GCCATCCTCT TAGCAGTCCA 
CGCAAAACTA GTCTTGTTAT CAATGATCTC GGGGATGAAG TTCATCTCAA GCCAGATAGC 
AAGGGCCCGG CGAAGCCAGG CCTGTTCAAG AACCCCATGC TGCAGTCTGA ATTGCCCTGT 
AGTCCCAGAG TGCCTGACTC TTTGGCTGCT AAAACAGCCC CCAAAGAAAT ATACCTAAAA 
AGAGCAGCGG GAGAAGATCC AGGCAACCCT CATCCTGCGC GAAGGCGCAA TGAGAAGGAG 
TACAGAAGCC CTGCAGAAGG AGAAAGGGCG CCGGCGGGGG CCCAGGGCTG CAGAGGCCCC 
CAGGATGCAG AGGACAGCGC AGGTGCCGAC ACCCAGGAGA AGTCTAACCG TGTGTTGCCC 
CTGAACAGTG AAGGTCAAGT GAACCTGTCT TGGTTAGGGA ATGATGCGCT GCTTCCTGCC 
CAACCCGTGC TGCAGGCAAC ACGTGCAGAA CTCTACTGCA TCACAGACTT TGCGGAAGAA 
TTAGCCGAGA TGATTGTCTC CATGGCAACC GAAATCGCAG CAATTTGCCT TGACAACTCA 
AATGGAAAAC AACCCTGGTT TTGTGCATGG AAGAGAGGGA ATGAGTTTCT GGTTACCCCC 
AACGTATCCT GCCGGTCCCT GAAGAGGAAG AAGGAAAGCC AGCCCGGCGG GGCCACCGTG 
AGGAAGCACA AGCCACCCCG GCTCAGCGAG ATCAAGAGGA AAACGGATGA GCACCCGGAG 
CTTAAGGAGA AGCTGATGAA CAGGGTCGTG GACGAGTCGA TGAACCTCGA GGACGTCCCA 
GATTCTGTCA ATATTTTTGC GAACGAAGTG GCCGCCAAGA TCATGAACCT AACGGACTTC 
TCCATGGTAG ACGGTGTCTG GCAAGCCCAG AGCCACCCCC GCACCCGGTT ACTCAGCGGA 
GACAGGTGGA GCCGACTGAA GGCCTCCAGC TGTGAAAGCA TTCCAGAGGA GGACTCTAAC 
GCCAGGGCTT ATGTAAATAG CCTGGGTTTC ATGAGTACCC TAAGTCAGCC AGTGAGCAGG 
GCCAGCTCTG TCTCCAAGCA GTCCAGCTGT GAGAGCATCA CTGAGGAGTT TTCCAGGTTC 
ATGGTGAACC AGATGGAAAA TGAAGGAAGA GGGTTTGAGT TACTGCTGGA TTACTATGCG 
GGCAAGAATG CCACCAGCAT CCTGAACTCA GCCATGCACC AGGCCTGCCA TAAAAATGAC 
CACCTCAGTG TGAGGCCGAG CTGTCCCTCG AAGCAGTCCA GCACCGAGAG CATAACCGAG 
GAGTTTTATA GGTACATGCT GAGGGACATT GAAAGAGAAA GCAGGGACAG CGCCTCCTCC 
AGACGAAGCA GCCAAGATTG GACAGTTGGC TTACTGTCAC CCTCTCTGCG ATCCCCATTG 
TGTTACAGAC AGTCATCTGT GCCCGATAGC CGATCCCTGG GTGCCAGGCT GACGGTGAAC 
CCACCCATCA AAGCCAACTC TTTAGATGGC TTCGCTCAAA ACAGCCAGCA AGATTTCCTA 
AGTGTACATC CAGTCAGTAG CACTTCCTCC TCGGGTCTCT GCAAATCTGA CTCTTGCTTG 
TATCGCAGAG GCGGAACTGA CCACATCACC AACATGTTAA TTCATGAGAC CTGGGCAAAC 
TCCATCAAGG CCCTCATGCA GAAGAACAAA ATTCTAGTGG ACGATGCCGA GGCAGCCGAT 
GCTGAGCCCG TTCCTGGTGG CTCTCCCATG CAAGTGGAGA AATGTGCAAA CAGATCGGCT 
TTGAGCAGAG TGCAAGGTGG GCCAGTTCTT CTTGTTCAGG AGTCTGTTGA TACCCCCAGG 
AAAAACTCCA TTACCGACAG CAAACATCCC CCAGTATCAT CTAAGAGCAA AGCTGCTCCT 
CTGACAAACC ACAACAGTTT AGATTCTAAG AAAGAATCTT CTTCGTGCCA TGGATCTGTG 
CCTGTCAGCC ACACCAAGCG GTCACTTTGC TCAAGGGAAG TGCCTTTAAT TCAGATTGAA 
ACAGATCAGA GAGAAGAGTG TGTTGGAGAA CCTGAACATT CCCCTTCCAA AAGCAGCCCC 
CTAGAGGAAG CAGAGGAGCA TTTGAAAGAT GCAAAAGTCC CAGAGGTGGC GAGGGGTGGA 
GACACTGCTG AGAGTGCCTG CCAAAGTCCT AGTGACAATC TTGATGCCAG CGATGTACCA 
GGGGCTGAAG TCTCGGCGGA AGGCAGAGCC CCCGATGAGT TCCCCAACCT TTCCGGCAGC 
AGTGGGGAGA GCACAGACAG CTGGTCCCAG CTCGCCAATG AGGAGGACAA TCCAGATGAC 
ACAAGTAGTT TTCTGCAGCT CAGTGAGCGA TCCATGAGTG AATTGGTGGA AGAAAAGGAG 
ATTCTTAAAG GACAGTCAGA AAACGTAGAG GAAGGTGCCT CTGGAGCGCC AATGGGAGTA 
GCCAGCTCCC AGGGGAGCCT GCTGGTGATC AACTTTGACC TGGAGCCAGA ATGTCCTGAT 
GCTGAGCTTC GAGCCACTCT GCAGTGGATA GCAGCCTCTG AACTTGGGAT CCCCACCATC 
TACTTTAAGA AATCTCAGGA AAACAGAATT GAAAAGTTTC TAGATGTTGT GCGACTGGTT 
CAGCAGAAGT CCTGGAAAGT GGGAGATATC TTCCATGCGG TTGTTCAGTA CTGCAAAATG 
CATGAGGAGC AGAAGGAGGG GACCCCAAGT CTCTTTGACT GGCTCTTGGA ACTGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635853.1
CDS	271..5370
Translation	MATPGSPCPAMWVVEPKSPTNLSAALSTRRHSQESSNLESPLMYEVSAPQQGSASGSLRGSLGSSITACKKVLCSNSLLESTDYWLQNQRTPCQIGFVEDKSENCASVCFVNLDVNKDACSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVLLSGLTSGNIQADFEVSQCPWLPDICLVQCARGNRANSTNCIIFEINKFLIGLELVQERQLHLETNVLKVEDDTNCSLSSIEEDFLTASEHLEEESEVEDLRNGYENISVSANVLENKKTKEATQEEWNYNKEKLLYALEDKYIGKYHPTLMKAEGSLEKVEDTTLQSLGTSAEPLGQKGEAVWNERPAPNYYYSENVKGHVEKSQAPHTPGDAYFSRIVKNSGPPVYEHDNSLHPGDHEDGTNPLPPKQDGEATTGEYATNLAESVLQDAFIRLSQCQPTPPQESAVSVSVGNAVLPSGCSTKGMVVPRSWNELPKIVIVQSPDGSDVAPEAAITSWSDMEVSVETSGVFSGENSSRHPQSALEVALACAATVIGTISSPQATERLKMEQESLVSTHPLGGSEPLQTQISQVLKEPSISEYSFPSALCGMTQVASAIAVCGLGETGEAKAPSGLSGTAEACAVVPTPCSLATGRSMELGNEAIARALLKEAALLLTRPESCSSVGDLMESMSERIIEAASKPQTLCSENVLRNEVAQTLSSVILKHSIDEARQKSKIIDPNDTRHSPETLDTWMESTNQLLFSVLCFTFKKMSHFVQLGECPAVLVNETSNWKEPELGYWPPDQAASQAWTKASEYSSSHPLSSPRKTSLVINDLGDEVHLKPDSKGPAKPGLFKNPMLQSELPCSPRVPDSLAAKTAPKEIYLKRAAGEDPGNPHPARRRNEKEYRSPAEGERAPAGAQGCRGPQDAEDSAGADTQEKSNRVLPLNSEGQVNLSWLGNDALLPAQPVLQATRAELYCITDFAEELAEMIVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLVTPNVSCRSLKRKKESQPGGATVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNIFANEVAAKIMNLTDFSMVDGVWQAQSHPRTRLLSGDRWSRLKASSCESIPEEDSNARAYVNSLGFMSTLSQPVSRASSVSKQSSCESITEEFSRFMVNQMENEGRGFELLLDYYAGKNATSILNSAMHQACHKNDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERESRDSASSRRSSQDWTVGLLSPSLRSPLCYRQSSVPDSRSLGARLTVNPPIKANSLDGFAQNSQQDFLSVHPVSSTSSSGLCKSDSCLYRRGGTDHITNMLIHETWANSIKALMQKNKILVDDAEAADAEPVPGGSPMQVEKCANRSALSRVQGGPVLLVQESVDTPRKNSITDSKHPPVSSKSKAAPLTNHNSLDSKKESSSCHGSVPVSHTKRSLCSREVPLIQIETDQREECVGEPEHSPSKSSPLEEAEEHLKDAKVPEVARGGDTAESACQSPSDNLDASDVPGAEVSAEGRAPDEFPNLSGSSGESTDSWSQLANEEDNPDDTSSFLQLSERSMSELVEEKEILKGQSENVEEGASGAPMGVASSQGSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVRLVQQKSWKVGDIFHAVVQYCKMHEEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_005635796.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635796.2

ORF Insert Sequence:

ATGGCAACTC CCGGCTCTCC GTGCCCAGCC ATGTGGGTAG TTGAACCAAA GTCACCTACC 
AACCTCTCTG CTGCCTTGTC CACCAGGAGA CACTCCCAGG AATCCAGCAA TTTGGAGTCA 
CCACTGATGT ACGAGGTTTC AGCGCCACAG CAGGGCAGCG CCAGCGGCAG CTTGAGAGGC 
AGCCTGGGTA GCTCCATCAC AGCGTGTAAG AAGGTTCTTT GCAGCAACAG CCTGCTTGAG 
TCTACAGACT ACTGGTTGCA GAATCAAAGG ACACCTTGCC AGATTGGTTT TGTGGAAGAC 
AAGTCTGAAA ACTGCGCTTC TGTTTGCTTC GTGAACCTGG ATGTGAACAA GGATGCATGC 
AGCACAGAGC ACCTGCAGCA GAAATTGGTC AATGTTTCAC CAGATCTTCC AAAACTCATC 
AGCTCCATGA ACGTCCAACA GCCAAAAGAA AATGAAATTG TCCTTCTAAG TGGGTTAACA 
TCTGGAAATA TCCAGGCAGA TTTTGAAGTT TCACAGTGTC CTTGGCTGCC AGATATCTGC 
TTGGTCCAGT GTGCGAGAGG GAACAGGGCA AACAGTACCA ATTGCATCAT CTTTGAGATC 
AACAAATTTT TGATTGGTCT GGAACTGGTG CAGGAGCGGC AGCTCCACCT GGAAACAAAC 
GTCCTGAAAG TGGAGGATGA CACGAACTGT TCCCTGTCTT CCATCGAGGA GGACTTTCTC 
ACCGCCTCTG AGCACTTGGA GGAGGAAAGT GAGGTGGAAG ATTTGAGGAA CGGTTATGAA 
AACATAAGTG TCTCAGCTAA TGTTTTGGAA AATAAAAAAA CAAAGGAAGC CACTCAGGAA 
GAATGGAATT ACAACAAGGA AAAGTTACTT TATGCTTTGG AAGACAAATA TATTGGCAAA 
TATCATCCGA CGTTGATGAA AGCAGAAGGA TCTCTGGAAA AAGTAGAAGA CACCACTTTG 
CAGAGCCTGG GCACATCAGC TGAGCCATTG GGACAGAAAG GTGAAGCTGT GTGGAATGAG 
AGGCCAGCCC CAAATTATTA TTATTCGGAA AATGTTAAAG GTCACGTGGA AAAATCTCAG 
GCACCACATA CTCCCGGGGA TGCTTATTTC TCCAGGATAG TTAAGAACAG TGGGCCTCCT 
GTATATGAGC ATGACAACAG CTTACACCCA GGGGACCATG AAGATGGCAC AAACCCCCTT 
CCTCCCAAAC AAGATGGAGA AGCCACAACT GGCGAGTATG CTACAAATTT AGCAGAATCC 
GTGCTGCAAG ATGCATTTAT TCGGTTGTCC CAGTGTCAGC CTACACCTCC CCAGGAATCG 
GCAGTCAGTG TTTCTGTCGG AAATGCTGTG CTACCCAGTG GCTGTTCCAC AAAAGGTATG 
GTGGTCCCTC GGTCATGGAA TGAGCTCCCC AAAATTGTCA TTGTTCAGAG TCCAGATGGC 
AGTGACGTCG CCCCGGAGGC AGCCATCACC TCATGGTCTG ACATGGAAGT CTCTGTTGAA 
ACCTCAGGTG TCTTCTCTGG AGAGAACTCC AGCAGACACC CTCAGAGTGC TCTAGAAGTT 
GCCTTGGCCT GTGCGGCCAC TGTAATTGGA ACCATTTCCA GTCCACAGGC CACAGAAAGG 
CTCAAAATGG AGCAAGAATC TTTGGTTTCA ACGCATCCAC TGGGAGGCAG TGAACCACTG 
CAAACTCAAA TATCCCAAGT ACTCAAGGAG CCTTCCATCA GCGAATACTC CTTTCCATCT 
GCTTTGTGTG GCATGACTCA GGTGGCGAGT GCCATTGCTG TTTGTGGCCT GGGTGAAACA 
GGAGAGGCCA AGGCTCCCAG TGGCCTCTCG GGTACAGCTG AGGCCTGTGC AGTAGTTCCC 
ACACCTTGCA GTCTGGCGAC AGGGAGGAGC ATGGAGCTGG GAAATGAGGC CATTGCACGA 
GCATTGCTCA AGGAGGCCGC TCTGCTTTTA ACAAGGCCAG AATCTTGCAG CAGCGTAGGA 
GACCTCATGG AATCAATGAG CGAGAGAATC ATTGAGGCTG CTTCAAAGCC TCAGACCTTG 
TGCTCAGAAA ACGTCCTCAG GAATGAAGTA GCACAGACCC TGTCCAGTGT TATTCTGAAG 
CATTCCATTG ATGAAGCTCG CCAGAAAAGC AAAATAATTG ATCCCAACGA TACTAGGCAT 
TCTCCAGAGA CTCTTGACAC CTGGATGGAA AGTACAAATC AACTGCTCTT CAGTGTGCTT 
TGCTTCACAT TCAAGAAGAT GAGCCATTTT GTACAGCTTG GTGAGTGCCC CGCCGTCCTT 
GTTAATGAGA CCAGCAACTG GAAAGAACCA GAATTAGGCT ACTGGCCGCC TGACCAGGCT 
GCCAGTCAAG CATGGACCAA AGCCTCTGAA TATTCCAGCA GCCATCCTCT TAGCAGTCCA 
CGCAAAACTA GTCTTGTTAT CAATGATCTC GGGGATGAAG TTCATCTCAA GCCAGATAGC 
AAGGGCCCGG CGAAGCCAGG CCTGTTCAAG AACCCCATGC TGCAGTCTGA ATTGCCCTGT 
AGTCCCAGAG TGCCTGACTC TTTGGCTGCT AAAACAGCCC CCAAAGAAAT ATACCTAAAA 
AGAGCAGCGG GAGAAGATCC AGGCAACCCT CATCCTGCGC GAAGGCGCAA TGAGAAGGAG 
TACAGAAGCC CTGCAGAAGG AGAAAGGGCG CCGGCGGGGG CCCAGGGCTG CAGAGGCCCC 
CAGGATGCAG AGGACAGCGC AGGTGCCGAC ACCCAGGAGA AGTCTAACCG TGTGTTGCCC 
CTGAACAGTG AAGGTCAAGT GAACCTGTCT TGGTTAGGGA ATGATGCGCT GCTTCCTGCC 
CAACCCGTGC TGCAGGCAAC ACGTGCAGAA CTCTACTGCA TCACAGACTT TGCGGAAGAA 
TTAGCCGAGA TGATTGTCTC CATGGCAACC GAAATCGCAG CAATTTGCCT TGACAACTCA 
AATGGAAAAC AACCCTGGTT TTGTGCATGG AAGAGAGGGA ATGAGTTTCT GGTTACCCCC 
AACGTATCCT GCCGGTCCCT GAAGAGGAAG AAGGAAAGCC AGCCCGGCGG GGCCACCGTG 
AGGAAGCACA AGCCACCCCG GCTCAGCGAG ATCAAGAGGA AAACGGATGA GCACCCGGAG 
CTTAAGGAGA AGCTGATGAA CAGGGTCGTG GACGAGTCGA TGAACCTCGA GGACGTCCCA 
GATTCTGTCA ATATTTTTGC GAACGAAGTG GCCGCCAAGA TCATGAACCT AACGGACTTC 
TCCATGGTAG ACGGTGTCTG GCAAGCCCAG AGCCACCCCC GCACCCGGTT ACTCAGCGGA 
GACAGGTGGA GCCGACTGAA GGCCTCCAGC TGTGAAAGCA TTCCAGAGGA GGACTCTAAC 
GCCAGGGCTT ATGTAAATAG CCTGGGTTTC ATGAGTACCC TAAGTCAGCC AGTGAGCAGG 
GCCAGCTCTG TCTCCAAGCA GTCCAGCTGT GAGAGCATCA CTGAGGAGTT TTCCAGGTTC 
ATGGTGAACC AGATGGAAAA TGAAGGAAGA GGGTTTGAGT TACTGCTGGA TTACTATGCG 
GGCAAGAATG CCACCAGCAT CCTGAACTCA GCCATGCACC AGGCCTGCCA TAAAAATGAC 
CACCTCAGTG TGAGGCCGAG CTGTCCCTCG AAGCAGTCCA GCACCGAGAG CATAACCGAG 
GAGTTTTATA GGTACATGCT GAGGGACATT GAAAGAGAAA GCAGGGACAG CGCCTCCTCC 
AGACGAAGCA GCCAAGATTG GACAGTTGGC TTACTGTCAC CCTCTCTGCG ATCCCCATTG 
TGTTACAGAC AGTCATCTGT GCCCGATAGC CGATCCCTGG GTGCCAGGCT GACGGTGAAC 
CCACCCATCA AAGCCAACTC TTTAGATGGC TTCGCTCAAA ACAGCCAGCA AGATTTCCTA 
AGTGTACATC CAGTCAGTAG CACTTCCTCC TCGGGTCTCT GCAAATCTGA CTCTTGCTTG 
TATCGCAGAG GCGGAACTGA CCACATCACC AACATGTTAA TTCATGAGAC CTGGGCAAAC 
TCCATCAAGG CCCTCATGCA GAAGAACAAA ATTCTAGTGG ACGATGCCGA GGCAGCCGAT 
GCTGAGCCCG TTCCTGGTGG CTCTCCCATG CAAGTGGAGA AATGTGCAAA CAGATCGGCT 
TTGAGCAGAG TGCAAGGTGG GCCAGTTCTT CTTGTTCAGG AGTCTGTTGA TACCCCCAGG 
AAAAACTCCA TTACCGACAG CAAACATCCC CCAGTATCAT CTAAGAGCAA AGCTGCTCCT 
CTGACAAACC ACAACAGTTT AGATTCTAAG AAAGAATCTT CTTCGTGCCA TGGATCTGTG 
CCTGTCAGCC ACACCAAGCG GTCACTTTGC TCAAGGGAAG TGCCTTTAAT TCAGATTGAA 
ACAGATCAGA GAGAAGAGTG TGTTGGAGAA CCTGAACATT CCCCTTCCAA AAGCAGCCCC 
CTAGAGGAAG CAGAGGAGCA TTTGAAAGAT GCAAAAGTCC CAGAGGTGGC GAGGGGTGGA 
GACACTGCTG AGAGTGCCTG CCAAAGTCCT AGTGACAATC TTGATGCCAG CGATGTACCA 
GGGGCTGAAG TCTCGGCGGA AGGCAGAGCC CCCGATGAGT TCCCCAACCT TTCCGGCAGC 
AGTGGGGAGA GCACAGACAG CTGGTCCCAG CTCGCCAATG AGGAGGACAA TCCAGATGAC 
ACAAGTAGTT TTCTGCAGCT CAGTGAGCGA TCCATGAGTG AATTGGTGGA AGAAAAGGAG 
ATTCTTAAAG GACAGTCAGA AAACGTAGAG GAAGGTGCCT CTGGAGCGCC AATGGGAGTA 
GCCAGCTCCC AGGGGAGCCT GCTGGTGATC AACTTTGACC TGGAGCCAGA ATGTCCTGAT 
GCTGAGCTTC GAGCCACTCT GCAGTGGATA GCAGCCTCTG AACTTGGGAT CCCCACCATC 
TACTTTAAGA AATCTCAGGA AAACAGAATT GAAAAGTTTC TAGATGTTGT GCGACTGGTT 
CAGCAGAAGT CCTGGAAAGT GGGAGATATC TTCCATGCGG TTGTTCAGTA CTGCAAAATG 
CATGAGGAGC AGAAGGAGGG GACCCCAAGT CTCTTTGACT GGCTCTTGGA ACTGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_022409759.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265467.1 Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.

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CloneID	OCe297119
Clone ID Related Accession (Same CDS sequence)	XM_022409759.1
Accession Version	XM_022409759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	A-kinase anchor protein SPHKAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGAGG TTTCAGCGCC ACAGCAGGGC AGCGCCAGCG GCAGCTTGAG AGGCAGCCTG 
GGTAGCTCCA TCACAGCGTG TAAGAAGGTT CTTTGCAGCA ACAGCCTGCT TGAGTCTACA 
GACTACTGGT TGCAGAATCA AAGGACACCT TGCCAGATTG GTTTTGTGGA AGACAAGTCT 
GAAAACTGCG CTTCTGTTTG CTTCGTGAAC CTGGATGTGA ACAAGGATGC ATGCAGCACA 
GAGCACCTGC AGCAGAAATT GGTCAATGTT TCACCAGATC TTCCAAAACT CATCAGCTCC 
ATGAACGTCC AACAGCCAAA AGAAAATGAA ATTGTCCTTC TAAGTGGGTT AACATCTGGA 
AATATCCAGG CAGATTTTGA AGTTTCACAG TGTCCTTGGC TGCCAGATAT CTGCTTGGTC 
CAGTGTGCGA GAGGGAACAG GGCAAACAGT ACCAATTGCA TCATCTTTGA GATCAACAAA 
TTTTTGATTG GTCTGGAACT GGTGCAGGAG CGGCAGCTCC ACCTGGAAAC AAACGTCCTG 
AAAGTGGAGG ATGACACGAA CTGTTCCCTG TCTTCCATCG AGGAGGACTT TCTCACCGCC 
TCTGAGCACT TGGAGGAGGA AAGTGAGGTG GAAGATTTGA GGAACGGTTA TGAAAACATA 
AGTGTCTCAG CTAATGTTTT GGAAAATAAA AAAACAAAGG AAGCCACTCA GGAAGAATGG 
AATTACAACA AGGAAAAGTT ACTTTATGCT TTGGAAGACA AATATATTGG CAAATATCAT 
CCGACGTTGA TGAAAGCAGA AGGATCTCTG GAAAAAGTAG AAGACACCAC TTTGCAGAGC 
CTGGGCACAT CAGCTGAGCC ATTGGGACAG AAAGGTGAAG CTGTGTGGAA TGAGAGGCCA 
GCCCCAAATT ATTATTATTC GGAAAATGTT AAAGGTCACG TGGAAAAATC TCAGGCACCA 
CATACTCCCG GGGATGCTTA TTTCTCCAGG ATAGTTAAGA ACAGTGGGCC TCCTGTATAT 
GAGCATGACA ACAGCTTACA CCCAGGGGAC CATGAAGATG GCACAAACCC CCTTCCTCCC 
AAACAAGATG GAGAAGCCAC AACTGGCGAG TATGCTACAA ATTTAGCAGA ATCCGTGCTG 
CAAGATGCAT TTATTCGGTT GTCCCAGTGT CAGCCTACAC CTCCCCAGGA ATCGGCAGTC 
AGTGTTTCTG TCGGAAATGC TGTGCTACCC AGTGGCTGTT CCACAAAAGG TATGGTGGTC 
CCTCGGTCAT GGAATGAGCT CCCCAAAATT GTCATTGTTC AGAGTCCAGA TGGCAGTGAC 
GTCGCCCCGG AGGCAGCCAT CACCTCATGG TCTGACATGG AAGTCTCTGT TGAAACCTCA 
GGTGTCTTCT CTGGAGAGAA CTCCAGCAGA CACCCTCAGA GTGCTCTAGA AGTTGCCTTG 
GCCTGTGCGG CCACTGTAAT TGGAACCATT TCCAGTCCAC AGGCCACAGA AAGGCTCAAA 
ATGGAGCAAG AATCTTTGGT TTCAACGCAT CCACTGGGAG GCAGTGAACC ACTGCAAACT 
CAAATATCCC AAGTACTCAA GGAGCCTTCC ATCAGCGAAT ACTCCTTTCC ATCTGCTTTG 
TGTGGCATGA CTCAGGTGGC GAGTGCCATT GCTGTTTGTG GCCTGGGTGA AACAGGAGAG 
GCCAAGGCTC CCAGTGGCCT CTCGGGTACA GCTGAGGCCT GTGCAGTAGT TCCCACACCT 
TGCAGTCTGG CGACAGGGAG GAGCATGGAG CTGGGAAATG AGGCCATTGC ACGAGCATTG 
CTCAAGGAGG CCGCTCTGCT TTTAACAAGG CCAGAATCTT GCAGCAGCGT AGGAGACCTC 
ATGGAATCAA TGAGCGAGAG AATCATTGAG GCTGCTTCAA AGCCTCAGAC CTTGTGCTCA 
GAAAACGTCC TCAGGAATGA AGTAGCACAG ACCCTGTCCA GTGTTATTCT GAAGCATTCC 
ATTGATGAAG CTCGCCAGAA AAGCAAAATA ATTGATCCCA ACGATACTAG GCATTCTCCA 
GAGACTCTTG ACACCTGGAT GGAAAGTACA AATCAACTGC TCTTCAGTGT GCTTTGCTTC 
ACATTCAAGA AGATGAGCCA TTTTGTACAG CTTGGTGAGT GCCCCGCCGT CCTTGTTAAT 
GAGACCAGCA ACTGGAAAGA ACCAGAATTA GGCTACTGGC CGCCTGACCA GGCTGCCAGT 
CAAGCATGGA CCAAAGCCTC TGAATATTCC AGCAGCCATC CTCTTAGCAG TCCACGCAAA 
ACTAGTCTTG TTATCAATGA TCTCGGGGAT GAAGTTCATC TCAAGCCAGA TAGCAAGGGC 
CCGGCGAAGC CAGGCCTGTT CAAGAACCCC ATGCTGCAGT CTGAATTGCC CTGTAGTCCC 
AGAGTGCCTG ACTCTTTGGC TGCTAAAACA GCCCCCAAAG AAATATACCT AAAAAGAGCA 
GCGGGAGAAG ATCCAGGCAA CCCTCATCCT GCGCGAAGGC GCAATGAGAA GGAGTACAGA 
AGCCCTGCAG AAGGAGAAAG GGCGCCGGCG GGGGCCCAGG GCTGCAGAGG CCCCCAGGAT 
GCAGAGGACA GCGCAGGTGC CGACACCCAG GAGAAGTCTA ACCGTGTGTT GCCCCTGAAC 
AGTGAAGGTC AAGTGAACCT GTCTTGGTTA GGGAATGATG CGCTGCTTCC TGCCCAACCC 
GTGCTGCAGG CAACACGTGC AGAACTCTAC TGCATCACAG ACTTTGCGGA AGAATTAGCC 
GAGATGATTG TCTCCATGGC AACCGAAATC GCAGCAATTT GCCTTGACAA CTCAAATGGA 
AAACAACCCT GGTTTTGTGC ATGGAAGAGA GGGAATGAGT TTCTGGTTAC CCCCAACGTA 
TCCTGCCGGT CCCTGAAGAG GAAGAAGGAA AGCCAGCCCG GCGGGGCCAC CGTGAGGAAG 
CACAAGCCAC CCCGGCTCAG CGAGATCAAG AGGAAAACGG ATGAGCACCC GGAGCTTAAG 
GAGAAGCTGA TGAACAGGGT CGTGGACGAG TCGATGAACC TCGAGGACGT CCCAGATTCT 
GTCAATATTT TTGCGAACGA AGTGGCCGCC AAGATCATGA ACCTAACGGA CTTCTCCATG 
GTAGACGGTG TCTGGCAAGC CCAGAGCCAC CCCCGCACCC GGTTACTCAG CGGAGACAGG 
TGGAGCCGAC TGAAGGCCTC CAGCTGTGAA AGCATTCCAG AGGAGGACTC TAACGCCAGG 
GCTTATGTAA ATAGCCTGGG TTTCATGAGT ACCCTAAGTC AGCCAGTGAG CAGGGCCAGC 
TCTGTCTCCA AGCAGTCCAG CTGTGAGAGC ATCACTGAGG AGTTTTCCAG GTTCATGGTG 
AACCAGATGG AAAATGAAGG AAGAGGGTTT GAGTTACTGC TGGATTACTA TGCGGGCAAG 
AATGCCACCA GCATCCTGAA CTCAGCCATG CACCAGGCCT GCCATAAAAA TGACCACCTC 
AGTGTGAGGC CGAGCTGTCC CTCGAAGCAG TCCAGCACCG AGAGCATAAC CGAGGAGTTT 
TATAGGTACA TGCTGAGGGA CATTGAAAGA GAAAGCAGGG ACAGCGCCTC CTCCAGACGA 
AGCAGCCAAG ATTGGACAGT TGGCTTACTG TCACCCTCTC TGCGATCCCC ATTGTGTTAC 
AGACAGTCAT CTGTGCCCGA TAGCCGATCC CTGGGTGCCA GGCTGACGGT GAACCCACCC 
ATCAAAGCCA ACTCTTTAGA TGGCTTCGCT CAAAACAGCC AGCAAGATTT CCTAAGTGTA 
CATCCAGTCA GTAGCACTTC CTCCTCGGGT CTCTGCAAAT CTGACTCTTG CTTGTATCGC 
AGAGGCGGAA CTGACCACAT CACCAACATG TTAATTCATG AGACCTGGGC AAACTCCATC 
AAGGCCCTCA TGCAGAAGAA CAAAATTCTA GTGGACGATG CCGAGGCAGC CGATGCTGAG 
CCCGTTCCTG GTGGCTCTCC CATGCAAGTG GAGAAATGTG CAAACAGATC GGCTTTGAGC 
AGAGTGCAAG GTGGGCCAGT TCTTCTTGTT CAGGAGTCTG TTGATACCCC CAGGAAAAAC 
TCCATTACCG ACAGCAAACA TCCCCCAGTA TCATCTAAGA GCAAAGCTGC TCCTCTGACA 
AACCACAACA GTTTAGATTC TAAGAAAGAA TCTTCTTCGT GCCATGGATC TGTGCCTGTC 
AGCCACACCA AGCGGTCACT TTGCTCAAGG GAAGTGCCTT TAATTCAGAT TGAAACAGAT 
CAGAGAGAAG AGTGTGTTGG AGAACCTGAA CATTCCCCTT CCAAAAGCAG CCCCCTAGAG 
GAAGCAGAGG AGCATTTGAA AGATGCAAAA GTCCCAGAGG TGGCGAGGGG TGGAGACACT 
GCTGAGAGTG CCTGCCAAAG TCCTAGTGAC AATCTTGATG CCAGCGATGT ACCAGGGGCT 
GAAGTCTCGG CGGAAGGCAG AGCCCCCGAT GAGTTCCCCA ACCTTTCCGG CAGCAGTGGG 
GAGAGCACAG ACAGCTGGTC CCAGCTCGCC AATGAGGAGG ACAATCCAGA TGACACAAGT 
AGTTTTCTGC AGCTCAGTGA GCGATCCATG AGATGCAACT CAGGCTGGGT TTACATGAGC 
AATGGCAACA GTAGTGCCAC TAGCAGTCTT GGCACTATGG ACCTGGACAT TTATCAGGAA 
AGCATGCCAT CTTCTCCCAT GATTAATGAA TTGGTGGAAG AAAAGGAGAT TCTTAAAGGA 
CAGTCAGAAA ACGTAGAGGA AGGTGCCTCT GGAGCGCCAA TGGGAGTAGC CAGCTCCCAG 
GGGAGCCTGC TGGTGATCAA CTTTGACCTG GAGCCAGAAT GTCCTGATGC TGAGCTTCGA 
GCCACTCTGC AGTGGATAGC AGCCTCTGAA CTTGGGATCC CCACCATCTA CTTTAAGAAA 
TCTCAGGAAA ACAGAATTGA AAAGTTTCTA GATGTTGTGC GACTGGTTCA GCAGAAGTCC 
TGGAAAGTGG GAGATATCTT CCATGCGGTT GTTCAGTACT GCAAAATGCA TGAGGAGCAG 
AAGGAGGGGA CCCCAAGTCT CTTTGACTGG CTCTTGGAAC TGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265467.1
CDS	136..5223
Translation	MYEVSAPQQGSASGSLRGSLGSSITACKKVLCSNSLLESTDYWLQNQRTPCQIGFVEDKSENCASVCFVNLDVNKDACSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVLLSGLTSGNIQADFEVSQCPWLPDICLVQCARGNRANSTNCIIFEINKFLIGLELVQERQLHLETNVLKVEDDTNCSLSSIEEDFLTASEHLEEESEVEDLRNGYENISVSANVLENKKTKEATQEEWNYNKEKLLYALEDKYIGKYHPTLMKAEGSLEKVEDTTLQSLGTSAEPLGQKGEAVWNERPAPNYYYSENVKGHVEKSQAPHTPGDAYFSRIVKNSGPPVYEHDNSLHPGDHEDGTNPLPPKQDGEATTGEYATNLAESVLQDAFIRLSQCQPTPPQESAVSVSVGNAVLPSGCSTKGMVVPRSWNELPKIVIVQSPDGSDVAPEAAITSWSDMEVSVETSGVFSGENSSRHPQSALEVALACAATVIGTISSPQATERLKMEQESLVSTHPLGGSEPLQTQISQVLKEPSISEYSFPSALCGMTQVASAIAVCGLGETGEAKAPSGLSGTAEACAVVPTPCSLATGRSMELGNEAIARALLKEAALLLTRPESCSSVGDLMESMSERIIEAASKPQTLCSENVLRNEVAQTLSSVILKHSIDEARQKSKIIDPNDTRHSPETLDTWMESTNQLLFSVLCFTFKKMSHFVQLGECPAVLVNETSNWKEPELGYWPPDQAASQAWTKASEYSSSHPLSSPRKTSLVINDLGDEVHLKPDSKGPAKPGLFKNPMLQSELPCSPRVPDSLAAKTAPKEIYLKRAAGEDPGNPHPARRRNEKEYRSPAEGERAPAGAQGCRGPQDAEDSAGADTQEKSNRVLPLNSEGQVNLSWLGNDALLPAQPVLQATRAELYCITDFAEELAEMIVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLVTPNVSCRSLKRKKESQPGGATVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNIFANEVAAKIMNLTDFSMVDGVWQAQSHPRTRLLSGDRWSRLKASSCESIPEEDSNARAYVNSLGFMSTLSQPVSRASSVSKQSSCESITEEFSRFMVNQMENEGRGFELLLDYYAGKNATSILNSAMHQACHKNDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERESRDSASSRRSSQDWTVGLLSPSLRSPLCYRQSSVPDSRSLGARLTVNPPIKANSLDGFAQNSQQDFLSVHPVSSTSSSGLCKSDSCLYRRGGTDHITNMLIHETWANSIKALMQKNKILVDDAEAADAEPVPGGSPMQVEKCANRSALSRVQGGPVLLVQESVDTPRKNSITDSKHPPVSSKSKAAPLTNHNSLDSKKESSSCHGSVPVSHTKRSLCSREVPLIQIETDQREECVGEPEHSPSKSSPLEEAEEHLKDAKVPEVARGGDTAESACQSPSDNLDASDVPGAEVSAEGRAPDEFPNLSGSSGESTDSWSQLANEEDNPDDTSSFLQLSERSMRCNSGWVYMSNGNSSATSSLGTMDLDIYQESMPSSPMINELVEEKEILKGQSENVEEGASGAPMGVASSQGSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVRLVQQKSWKVGDIFHAVVQYCKMHEEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_022409759.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409759.1

ORF Insert Sequence:

ATGTACGAGG TTTCAGCGCC ACAGCAGGGC AGCGCCAGCG GCAGCTTGAG AGGCAGCCTG 
GGTAGCTCCA TCACAGCGTG TAAGAAGGTT CTTTGCAGCA ACAGCCTGCT TGAGTCTACA 
GACTACTGGT TGCAGAATCA AAGGACACCT TGCCAGATTG GTTTTGTGGA AGACAAGTCT 
GAAAACTGCG CTTCTGTTTG CTTCGTGAAC CTGGATGTGA ACAAGGATGC ATGCAGCACA 
GAGCACCTGC AGCAGAAATT GGTCAATGTT TCACCAGATC TTCCAAAACT CATCAGCTCC 
ATGAACGTCC AACAGCCAAA AGAAAATGAA ATTGTCCTTC TAAGTGGGTT AACATCTGGA 
AATATCCAGG CAGATTTTGA AGTTTCACAG TGTCCTTGGC TGCCAGATAT CTGCTTGGTC 
CAGTGTGCGA GAGGGAACAG GGCAAACAGT ACCAATTGCA TCATCTTTGA GATCAACAAA 
TTTTTGATTG GTCTGGAACT GGTGCAGGAG CGGCAGCTCC ACCTGGAAAC AAACGTCCTG 
AAAGTGGAGG ATGACACGAA CTGTTCCCTG TCTTCCATCG AGGAGGACTT TCTCACCGCC 
TCTGAGCACT TGGAGGAGGA AAGTGAGGTG GAAGATTTGA GGAACGGTTA TGAAAACATA 
AGTGTCTCAG CTAATGTTTT GGAAAATAAA AAAACAAAGG AAGCCACTCA GGAAGAATGG 
AATTACAACA AGGAAAAGTT ACTTTATGCT TTGGAAGACA AATATATTGG CAAATATCAT 
CCGACGTTGA TGAAAGCAGA AGGATCTCTG GAAAAAGTAG AAGACACCAC TTTGCAGAGC 
CTGGGCACAT CAGCTGAGCC ATTGGGACAG AAAGGTGAAG CTGTGTGGAA TGAGAGGCCA 
GCCCCAAATT ATTATTATTC GGAAAATGTT AAAGGTCACG TGGAAAAATC TCAGGCACCA 
CATACTCCCG GGGATGCTTA TTTCTCCAGG ATAGTTAAGA ACAGTGGGCC TCCTGTATAT 
GAGCATGACA ACAGCTTACA CCCAGGGGAC CATGAAGATG GCACAAACCC CCTTCCTCCC 
AAACAAGATG GAGAAGCCAC AACTGGCGAG TATGCTACAA ATTTAGCAGA ATCCGTGCTG 
CAAGATGCAT TTATTCGGTT GTCCCAGTGT CAGCCTACAC CTCCCCAGGA ATCGGCAGTC 
AGTGTTTCTG TCGGAAATGC TGTGCTACCC AGTGGCTGTT CCACAAAAGG TATGGTGGTC 
CCTCGGTCAT GGAATGAGCT CCCCAAAATT GTCATTGTTC AGAGTCCAGA TGGCAGTGAC 
GTCGCCCCGG AGGCAGCCAT CACCTCATGG TCTGACATGG AAGTCTCTGT TGAAACCTCA 
GGTGTCTTCT CTGGAGAGAA CTCCAGCAGA CACCCTCAGA GTGCTCTAGA AGTTGCCTTG 
GCCTGTGCGG CCACTGTAAT TGGAACCATT TCCAGTCCAC AGGCCACAGA AAGGCTCAAA 
ATGGAGCAAG AATCTTTGGT TTCAACGCAT CCACTGGGAG GCAGTGAACC ACTGCAAACT 
CAAATATCCC AAGTACTCAA GGAGCCTTCC ATCAGCGAAT ACTCCTTTCC ATCTGCTTTG 
TGTGGCATGA CTCAGGTGGC GAGTGCCATT GCTGTTTGTG GCCTGGGTGA AACAGGAGAG 
GCCAAGGCTC CCAGTGGCCT CTCGGGTACA GCTGAGGCCT GTGCAGTAGT TCCCACACCT 
TGCAGTCTGG CGACAGGGAG GAGCATGGAG CTGGGAAATG AGGCCATTGC ACGAGCATTG 
CTCAAGGAGG CCGCTCTGCT TTTAACAAGG CCAGAATCTT GCAGCAGCGT AGGAGACCTC 
ATGGAATCAA TGAGCGAGAG AATCATTGAG GCTGCTTCAA AGCCTCAGAC CTTGTGCTCA 
GAAAACGTCC TCAGGAATGA AGTAGCACAG ACCCTGTCCA GTGTTATTCT GAAGCATTCC 
ATTGATGAAG CTCGCCAGAA AAGCAAAATA ATTGATCCCA ACGATACTAG GCATTCTCCA 
GAGACTCTTG ACACCTGGAT GGAAAGTACA AATCAACTGC TCTTCAGTGT GCTTTGCTTC 
ACATTCAAGA AGATGAGCCA TTTTGTACAG CTTGGTGAGT GCCCCGCCGT CCTTGTTAAT 
GAGACCAGCA ACTGGAAAGA ACCAGAATTA GGCTACTGGC CGCCTGACCA GGCTGCCAGT 
CAAGCATGGA CCAAAGCCTC TGAATATTCC AGCAGCCATC CTCTTAGCAG TCCACGCAAA 
ACTAGTCTTG TTATCAATGA TCTCGGGGAT GAAGTTCATC TCAAGCCAGA TAGCAAGGGC 
CCGGCGAAGC CAGGCCTGTT CAAGAACCCC ATGCTGCAGT CTGAATTGCC CTGTAGTCCC 
AGAGTGCCTG ACTCTTTGGC TGCTAAAACA GCCCCCAAAG AAATATACCT AAAAAGAGCA 
GCGGGAGAAG ATCCAGGCAA CCCTCATCCT GCGCGAAGGC GCAATGAGAA GGAGTACAGA 
AGCCCTGCAG AAGGAGAAAG GGCGCCGGCG GGGGCCCAGG GCTGCAGAGG CCCCCAGGAT 
GCAGAGGACA GCGCAGGTGC CGACACCCAG GAGAAGTCTA ACCGTGTGTT GCCCCTGAAC 
AGTGAAGGTC AAGTGAACCT GTCTTGGTTA GGGAATGATG CGCTGCTTCC TGCCCAACCC 
GTGCTGCAGG CAACACGTGC AGAACTCTAC TGCATCACAG ACTTTGCGGA AGAATTAGCC 
GAGATGATTG TCTCCATGGC AACCGAAATC GCAGCAATTT GCCTTGACAA CTCAAATGGA 
AAACAACCCT GGTTTTGTGC ATGGAAGAGA GGGAATGAGT TTCTGGTTAC CCCCAACGTA 
TCCTGCCGGT CCCTGAAGAG GAAGAAGGAA AGCCAGCCCG GCGGGGCCAC CGTGAGGAAG 
CACAAGCCAC CCCGGCTCAG CGAGATCAAG AGGAAAACGG ATGAGCACCC GGAGCTTAAG 
GAGAAGCTGA TGAACAGGGT CGTGGACGAG TCGATGAACC TCGAGGACGT CCCAGATTCT 
GTCAATATTT TTGCGAACGA AGTGGCCGCC AAGATCATGA ACCTAACGGA CTTCTCCATG 
GTAGACGGTG TCTGGCAAGC CCAGAGCCAC CCCCGCACCC GGTTACTCAG CGGAGACAGG 
TGGAGCCGAC TGAAGGCCTC CAGCTGTGAA AGCATTCCAG AGGAGGACTC TAACGCCAGG 
GCTTATGTAA ATAGCCTGGG TTTCATGAGT ACCCTAAGTC AGCCAGTGAG CAGGGCCAGC 
TCTGTCTCCA AGCAGTCCAG CTGTGAGAGC ATCACTGAGG AGTTTTCCAG GTTCATGGTG 
AACCAGATGG AAAATGAAGG AAGAGGGTTT GAGTTACTGC TGGATTACTA TGCGGGCAAG 
AATGCCACCA GCATCCTGAA CTCAGCCATG CACCAGGCCT GCCATAAAAA TGACCACCTC 
AGTGTGAGGC CGAGCTGTCC CTCGAAGCAG TCCAGCACCG AGAGCATAAC CGAGGAGTTT 
TATAGGTACA TGCTGAGGGA CATTGAAAGA GAAAGCAGGG ACAGCGCCTC CTCCAGACGA 
AGCAGCCAAG ATTGGACAGT TGGCTTACTG TCACCCTCTC TGCGATCCCC ATTGTGTTAC 
AGACAGTCAT CTGTGCCCGA TAGCCGATCC CTGGGTGCCA GGCTGACGGT GAACCCACCC 
ATCAAAGCCA ACTCTTTAGA TGGCTTCGCT CAAAACAGCC AGCAAGATTT CCTAAGTGTA 
CATCCAGTCA GTAGCACTTC CTCCTCGGGT CTCTGCAAAT CTGACTCTTG CTTGTATCGC 
AGAGGCGGAA CTGACCACAT CACCAACATG TTAATTCATG AGACCTGGGC AAACTCCATC 
AAGGCCCTCA TGCAGAAGAA CAAAATTCTA GTGGACGATG CCGAGGCAGC CGATGCTGAG 
CCCGTTCCTG GTGGCTCTCC CATGCAAGTG GAGAAATGTG CAAACAGATC GGCTTTGAGC 
AGAGTGCAAG GTGGGCCAGT TCTTCTTGTT CAGGAGTCTG TTGATACCCC CAGGAAAAAC 
TCCATTACCG ACAGCAAACA TCCCCCAGTA TCATCTAAGA GCAAAGCTGC TCCTCTGACA 
AACCACAACA GTTTAGATTC TAAGAAAGAA TCTTCTTCGT GCCATGGATC TGTGCCTGTC 
AGCCACACCA AGCGGTCACT TTGCTCAAGG GAAGTGCCTT TAATTCAGAT TGAAACAGAT 
CAGAGAGAAG AGTGTGTTGG AGAACCTGAA CATTCCCCTT CCAAAAGCAG CCCCCTAGAG 
GAAGCAGAGG AGCATTTGAA AGATGCAAAA GTCCCAGAGG TGGCGAGGGG TGGAGACACT 
GCTGAGAGTG CCTGCCAAAG TCCTAGTGAC AATCTTGATG CCAGCGATGT ACCAGGGGCT 
GAAGTCTCGG CGGAAGGCAG AGCCCCCGAT GAGTTCCCCA ACCTTTCCGG CAGCAGTGGG 
GAGAGCACAG ACAGCTGGTC CCAGCTCGCC AATGAGGAGG ACAATCCAGA TGACACAAGT 
AGTTTTCTGC AGCTCAGTGA GCGATCCATG AGATGCAACT CAGGCTGGGT TTACATGAGC 
AATGGCAACA GTAGTGCCAC TAGCAGTCTT GGCACTATGG ACCTGGACAT TTATCAGGAA 
AGCATGCCAT CTTCTCCCAT GATTAATGAA TTGGTGGAAG AAAAGGAGAT TCTTAAAGGA 
CAGTCAGAAA ACGTAGAGGA AGGTGCCTCT GGAGCGCCAA TGGGAGTAGC CAGCTCCCAG 
GGGAGCCTGC TGGTGATCAA CTTTGACCTG GAGCCAGAAT GTCCTGATGC TGAGCTTCGA 
GCCACTCTGC AGTGGATAGC AGCCTCTGAA CTTGGGATCC CCACCATCTA CTTTAAGAAA 
TCTCAGGAAA ACAGAATTGA AAAGTTTCTA GATGTTGTGC GACTGGTTCA GCAGAAGTCC 
TGGAAAGTGG GAGATATCTT CCATGCGGTT GTTCAGTACT GCAAAATGCA TGAGGAGCAG 
AAGGAGGGGA CCCCAAGTCT CTTTGACTGG CTCTTGGAAC TGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SPHKAP cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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