CACNA1G cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1G
Entrez Gene ID	491083
Full Name	calcium voltage-gated channel subunit alpha1 G
Synonyms	Ca(v)3.1
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 G Names voltage-dependent T-type calcium channel subunit alpha-1G
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014116649.1	XP_013972124.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X28
XM_014116642.1	XP_013972117.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
XM_014116648.1	XP_013972123.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X25
XM_005624648.2	XP_005624705.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
XM_005624649.2	XP_005624706.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
XM_005624649.3	XP_005624706.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
XM_005624656.2	XP_005624713.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X21
XM_005624657.2	XP_005624714.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X22
XM_005624660.2	XP_005624717.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X27
XM_005624660.3	XP_005624717.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X23
XM_860610.4	XP_865703.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X24
XM_860625.4	XP_865718.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X17
XM_860714.5	XP_865807.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
XM_860802.5	XP_865895.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X27
XM_014116644.1	XP_013972119.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X14
XM_014116647.1	XP_013972122.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X23
XM_014116647.2	XP_013972122.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X19
XM_014116643.1	XP_013972118.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
XM_014116649.2	XP_013972124.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X24
XM_005624657.3	XP_005624714.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X18
XM_860640.5	XP_865733.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
XM_860654.5	XP_865747.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X25
XM_860688.5	XP_865781.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X17
XM_860703.5	XP_865796.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X16
XM_003435267.3	XP_003435315.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X26
XM_005624647.3	XP_005624704.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
XM_005624650.2	XP_005624707.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
XM_005624651.3	XP_005624708.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
XM_005624652.2	XP_005624709.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
XM_005624655.3	XP_005624712.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X15
XM_022423110.1	XP_022278818.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X30
XM_860594.5	XP_865687.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X26
XM_860610.5	XP_865703.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X20
XM_860640.4	XP_865733.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X15
XM_860703.4	XP_865796.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X19
XM_860714.4	XP_865807.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
XM_005624651.2	XP_005624708.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
XM_005624653.2	XP_005624710.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
XM_005624653.3	XP_005624710.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
XM_005624655.2	XP_005624712.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X18
XM_005624663.2	XP_005624720.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X32
XM_014116641.1	XP_013972116.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
XM_014116643.2	XP_013972118.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
XM_014116645.1	XP_013972120.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X16
XM_014116645.2	XP_013972120.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
XM_014116648.2	XP_013972123.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X21
XM_860688.4	XP_865781.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X20
XM_860769.4	XP_865862.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X33
XM_860769.5	XP_865862.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X29
XM_548203.6	XP_548203.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
XM_022423107.1	XP_022278815.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
XM_003435267.4	XP_003435315.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X22
XM_005624647.2	XP_005624704.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
XM_005624652.3	XP_005624709.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
XM_005624654.2	XP_005624711.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
XM_005624663.3	XP_005624720.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X28
XM_022423104.1	XP_022278812.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
XM_022423105.1	XP_022278813.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
XM_022423111.1	XP_022278819.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X31
XM_860802.4	XP_865895.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X31
XM_548203.5	XP_548203.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
XM_860594.4	XP_865687.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X30
XM_860625.5	XP_865718.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X14
XM_860654.4	XP_865747.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X29

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA1G	NP_938191.2
Danio rerio (zebrafish)	cacna1g	XP_005156372.1
Bos taurus (cattle)	CACNA1G	NP_001180069.1
Xenopus tropicalis (tropical clawed frog)	cacna1g	XP_004918544.1
Mus musculus (house mouse)	Cacna1g	NP_001106284.1
Rattus norvegicus (Norway rat)	Cacna1g	NP_113789.2
Pan troglodytes (chimpanzee)	CACNA1G	XP_511884.2
Gallus gallus (chicken)	CACNA1G	XP_004946372.1
Canis lupus familiaris (dog)	CACNA1G	XP_005624708.1

Gene Ontology

The following CACNA1G gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1G cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe290665	XM_022423110.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe290666	XM_022423111.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe11910	XM_005624647.3 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11911	XM_005624648.2 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11912	XM_005624649.3 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11913	XM_548203.6 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11914	XM_005624650.2 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11915	XM_005624651.3 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11916	XM_005624652.3 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11917	XM_005624653.3 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11918	XM_860714.5 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11919	XM_005624654.2 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11920	XM_860640.5 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11921	XM_860625.5 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11922	XM_005624655.3 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11923	XM_860703.5 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11924	XM_860688.5 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11925	XM_005624656.2 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11926	XM_005624657.3 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11927	XM_860610.5 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11929	XM_003435267.4 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11931	XM_005624660.3 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11932	XM_860654.5 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11933	XM_860594.5 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11936	XM_860802.5 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11937	XM_005624663.3 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11939	XM_860769.5 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe290662	XM_022423104.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe290663	XM_022423105.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe290664	XM_022423107.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe38390	XM_014116641.1 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe38391	XM_014116642.1 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe38392	XM_014116643.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe38393	XM_014116644.1 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe38394	XM_014116645.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe38395	XM_014116647.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe38396	XM_014116648.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe38397	XM_014116649.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCe11912	XM_005624649.2	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11931	XM_005624660.2	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe38395	XM_014116647.1	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe38397	XM_014116649.1	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11926	XM_005624657.2	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11927	XM_860610.4	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11920	XM_860640.4	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11923	XM_860703.4	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11918	XM_860714.4	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11915	XM_005624651.2	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11917	XM_005624653.2	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11922	XM_005624655.2	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe38392	XM_014116643.1	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe38394	XM_014116645.1	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe38396	XM_014116648.1	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11924	XM_860688.4	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11939	XM_860769.4	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11929	XM_003435267.3	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11910	XM_005624647.2	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11916	XM_005624652.2	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11937	XM_005624663.2	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11936	XM_860802.4	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11913	XM_548203.5	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe11933	XM_860594.4	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11921	XM_860625.4	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe11932	XM_860654.4	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1G ( XM_022423110.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022278818.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X30, mRNA.

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CloneID	OCe290665
Clone ID Related Accession (Same CDS sequence)	XM_022423110.1
Accession Version	XM_022423110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAATCT AATGCTGGAC GATGTAATTG CTTCCGGCAG CTCATCCAGC 
GCGGCGCCAG AGGCCCAGTG TAAACCCTAC TACTCAGACT ACTCCCGATT CCGGCTCCTC 
GTCCACCACT TGTGTACCAG CCACTATCTG GACCTCTTCA TCACGGGTGT CATTGGTCTG 
AATGTGGTCA CCATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA CGAGGCCTTG 
AAGATCTGCA ACTATATCTT CACCGTCATC TTTGTCTTGG AGTCAGTTTT TAAACTCGTG 
GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATCGTG 
CTGCTGTCCA TCATGGGCAT CACGCTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGAGTG CTGCGCATTG CCCGAGTGCT GAAGCTGCTG 
AAGATGGCTG TGGGCATGCG GGCGCTGCTG GACACAGTCA TGCAGGCCCT GCCTCAGCTC 
TGGGTGTGGA GCTCTTTGGA GACCTGGAGT GTGATGAGAC GCACCCCTGC GAGGGCCTGG 
GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022278818.1
CDS	504..5765
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQLWVWSSLETWSVMRRTPARAWAGMPPFGTLAWPS

Target ORF information:

RefSeq Version	XM_022423110.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423110.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAATCT AATGCTGGAC GATGTAATTG CTTCCGGCAG CTCATCCAGC 
GCGGCGCCAG AGGCCCAGTG TAAACCCTAC TACTCAGACT ACTCCCGATT CCGGCTCCTC 
GTCCACCACT TGTGTACCAG CCACTATCTG GACCTCTTCA TCACGGGTGT CATTGGTCTG 
AATGTGGTCA CCATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA CGAGGCCTTG 
AAGATCTGCA ACTATATCTT CACCGTCATC TTTGTCTTGG AGTCAGTTTT TAAACTCGTG 
GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATCGTG 
CTGCTGTCCA TCATGGGCAT CACGCTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGAGTG CTGCGCATTG CCCGAGTGCT GAAGCTGCTG 
AAGATGGCTG TGGGCATGCG GGCGCTGCTG GACACAGTCA TGCAGGCCCT GCCTCAGCTC 
TGGGTGTGGA GCTCTTTGGA GACCTGGAGT GTGATGAGAC GCACCCCTGC GAGGGCCTGG 
GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_022423111.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022278819.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X31, mRNA.

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CloneID	OCe290666
Clone ID Related Accession (Same CDS sequence)	XM_022423111.1
Accession Version	XM_022423111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAAGGC CCAGTGTAAA CCCTACTACT CAGACTACTC CCGATTCCGG 
CTCCTCGTCC ACCACTTGTG TACCAGCCAC TATCTGGACC TCTTCATCAC GGGTGTCATT 
GGTCTGAATG TGGTCACCAT GGCCATGGAG CACTACCAGC AGCCCCAGAT CCTGGACGAG 
GCCTTGAAGA TCTGCAACTA TATCTTCACC GTCATCTTTG TCTTGGAGTC AGTTTTTAAA 
CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC CAGGACAGGT GGAACCAGCT GGACCTGGCC 
ATCGTGCTGC TGTCCATCAT GGGCATCACG CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG 
CCCATCAACC CCACCATCAT CCGCATCATG AGAGTGCTGC GCATTGCCCG AGTGCTGAAG 
CTGCTGAAGA TGGCTGTGGG CATGCGGGCG CTGCTGGACA CAGTCATGCA GGCCCTGCCT 
CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC CCCTGCGAGG 
GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022278819.1
CDS	504..5711
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQLWVWSSLETWSVMRRTPARAWAGMPPFGTLAWPS

Target ORF information:

RefSeq Version	XM_022423111.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423111.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAAGGC CCAGTGTAAA CCCTACTACT CAGACTACTC CCGATTCCGG 
CTCCTCGTCC ACCACTTGTG TACCAGCCAC TATCTGGACC TCTTCATCAC GGGTGTCATT 
GGTCTGAATG TGGTCACCAT GGCCATGGAG CACTACCAGC AGCCCCAGAT CCTGGACGAG 
GCCTTGAAGA TCTGCAACTA TATCTTCACC GTCATCTTTG TCTTGGAGTC AGTTTTTAAA 
CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC CAGGACAGGT GGAACCAGCT GGACCTGGCC 
ATCGTGCTGC TGTCCATCAT GGGCATCACG CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG 
CCCATCAACC CCACCATCAT CCGCATCATG AGAGTGCTGC GCATTGCCCG AGTGCTGAAG 
CTGCTGAAGA TGGCTGTGGG CATGCGGGCG CTGCTGGACA CAGTCATGCA GGCCCTGCCT 
CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC CCCTGCGAGG 
GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624647.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624704.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X2, mRNA.

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CloneID	OCe11910
Clone ID Related Accession (Same CDS sequence)	XM_005624647.3 , XM_005624647.2
Accession Version	XM_005624647.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624647.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGAGGCCCA GTGTAAACCC 
TACTACTCAG ACTACTCCCG ATTCCGGCTC CTCGTCCACC ACTTGTGTAC CAGCCACTAT 
CTGGACCTCT TCATCACGGG TGTCATTGGT CTGAATGTGG TCACCATGGC CATGGAGCAC 
TACCAGCAGC CCCAGATCCT GGACGAGGCC TTGAAGATCT GCAACTATAT CTTCACCGTC 
ATCTTTGTCT TGGAGTCAGT TTTTAAACTC GTGGCCTTTG GCTTCCGTCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCCATC GTGCTGCTGT CCATCATGGG CATCACGCTG 
GAGGAGATTG AGGTCAATGC CTCGCTGCCC ATCAACCCCA CCATCATCCG CATCATGAGA 
GTGCTGCGCA TTGCCCGAGT GCTGAAGCTG CTGAAGATGG CTGTGGGCAT GCGGGCGCTG 
CTGGACACAG TCATGCAGGC CCTGCCTCAG GTGGGGAACC TGGGACTTCT CTTCATGTTG 
TTGTTTTTCA TCTTTGCAGC TCTGGGTGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACGCACCCCT GCGAGGGCCT GGGCCGGCAT GCCACCTTTC GGAACTTTGG CATGGCCTTC 
CTGACCCTCT TCCGAGTGTC CACAGGTGAC AACTGGAACG GCATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACATGCTAC AATACAGTCA TCTCCCCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TGATCGCTGT GCTGATGAAG 
CACCTGGAGG AAAGCAACAA GGAGGCCAAA GAGGAGGCCG AGCTGGAGGC TGAGCTGGAG 
CTGGAGATGA AGACGCTCAG CCCACAGCCC CACTCGCCAC TGGGCAGCCC CTTCCTCTGG 
CCGGGGGTCG AGGGCCCCGA CAGCCCCGAT AGCCCCAAGC TCAGGGCCCC GCACACCGCG 
GCCCACACGG GAGCAGCCTC GCGCTTCTCC CTGGAACACC CCACGGACAG ACAGCTGTTT 
GACACCATAT CCCTGCTGAT CCAGGGCTCC CTGGAAGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTCCCG CCCGCAGCCT GGGCGGCTCC 
GACCCACAGA TCCCTCTAGC TGAGATGGAG GCTCTGTCTC TGACGTCAGA GATTGTGTCT 
GAACCGTCCC ACTCTCTAGC TCTGACGGAT GACTCTTTGC CTGATGACAC TCACACACTC 
TTACCTAGTG CCCTGGACAG CAATATGGAG CCCCACCCCG AGGAGATGCC CCTTGCACTG 
GGACTGGACC TGCTGACTGT GAGGAAGTCT GGGGTCAGCC GGACACACTC CCTGCCCAAT 
GACAGCTACA TGTTCCGGGA CGGAGGCACC GCTGAGGGGT CCCTAGGATG CAGAAGCTGG 
GGGCTCCCCA AAGCTCAGTC AGGCTCTGTC TTATCTGTCC ACTCGCAGCC AGCAGACACC 
AGCTACATCC TGCAGCTTCC CAAAGACACA CCTCACCTGC TCCAGCCCCA CAGCGCTCCC 
ACCTGGGGCA CCATTCCCAA ACTGCCCCCA CCAGGCCGCT CCCCCTTGGC TCAGAGGCCA 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAT TCTCTGGACG TTCAGGGCCT GGGCAGCCGG 
GAAGACCTGC TGTCCGAGGT GAGTGGGCCC TCCCCACCTC TGGCCCGGGC CTCCTCCTTC 
TGGGGCCACT TGGGCGCCCA GATGCAGCAG CATCCCCACC GCCAGAGCAA GATCTCGAAG 
CACATGCCCC CGCCAGCCCC CCGCCCAGGC TCAGAAACCA CCTGGAGCAA AGACCCACCC 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
ACCGTGGGCA GCCAGGAGGA GCCCCCGTCC CCACGGGACC TGAAGAAGTG TCACAGCGTG 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGCG TCCTGGCTGG ATGAACAGAG GAGACACTCC 
ATCGCGGTCA GCTGCCTGGA CGGCGGCTCC CAACCCAGCC TGGGCCCCGG CCCCTCTAGC 
CTTGGCGGCC AGCCTCTTGG GGGACCTGGA AGCCGGCCCA AGAAAAAACT CAGCCCACCT 
AGTATCTCCA TAGACCCCCC AGAGAGTCAG GGCCCTCGGC CCCCACCCAG CCCCGGCGTC 
TGCCTCCGGC GGAGGGCTCC GTCCAGCGAC TCCAGGGACC CCTCGGCCTC CGGCCCCCCT 
GACAGCATGG CTGCCTCGCC CCCCCCAAAG AAAGACGTGC TGAGTCTCTC TGGTTTAGCC 
TCTGACCCAG CAGACCTGGA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624704.1
CDS	505..7671
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624647.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624647.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGAGGCCCA GTGTAAACCC 
TACTACTCAG ACTACTCCCG ATTCCGGCTC CTCGTCCACC ACTTGTGTAC CAGCCACTAT 
CTGGACCTCT TCATCACGGG TGTCATTGGT CTGAATGTGG TCACCATGGC CATGGAGCAC 
TACCAGCAGC CCCAGATCCT GGACGAGGCC TTGAAGATCT GCAACTATAT CTTCACCGTC 
ATCTTTGTCT TGGAGTCAGT TTTTAAACTC GTGGCCTTTG GCTTCCGTCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCCATC GTGCTGCTGT CCATCATGGG CATCACGCTG 
GAGGAGATTG AGGTCAATGC CTCGCTGCCC ATCAACCCCA CCATCATCCG CATCATGAGA 
GTGCTGCGCA TTGCCCGAGT GCTGAAGCTG CTGAAGATGG CTGTGGGCAT GCGGGCGCTG 
CTGGACACAG TCATGCAGGC CCTGCCTCAG GTGGGGAACC TGGGACTTCT CTTCATGTTG 
TTGTTTTTCA TCTTTGCAGC TCTGGGTGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACGCACCCCT GCGAGGGCCT GGGCCGGCAT GCCACCTTTC GGAACTTTGG CATGGCCTTC 
CTGACCCTCT TCCGAGTGTC CACAGGTGAC AACTGGAACG GCATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACATGCTAC AATACAGTCA TCTCCCCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TGATCGCTGT GCTGATGAAG 
CACCTGGAGG AAAGCAACAA GGAGGCCAAA GAGGAGGCCG AGCTGGAGGC TGAGCTGGAG 
CTGGAGATGA AGACGCTCAG CCCACAGCCC CACTCGCCAC TGGGCAGCCC CTTCCTCTGG 
CCGGGGGTCG AGGGCCCCGA CAGCCCCGAT AGCCCCAAGC TCAGGGCCCC GCACACCGCG 
GCCCACACGG GAGCAGCCTC GCGCTTCTCC CTGGAACACC CCACGGACAG ACAGCTGTTT 
GACACCATAT CCCTGCTGAT CCAGGGCTCC CTGGAAGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTCCCG CCCGCAGCCT GGGCGGCTCC 
GACCCACAGA TCCCTCTAGC TGAGATGGAG GCTCTGTCTC TGACGTCAGA GATTGTGTCT 
GAACCGTCCC ACTCTCTAGC TCTGACGGAT GACTCTTTGC CTGATGACAC TCACACACTC 
TTACCTAGTG CCCTGGACAG CAATATGGAG CCCCACCCCG AGGAGATGCC CCTTGCACTG 
GGACTGGACC TGCTGACTGT GAGGAAGTCT GGGGTCAGCC GGACACACTC CCTGCCCAAT 
GACAGCTACA TGTTCCGGGA CGGAGGCACC GCTGAGGGGT CCCTAGGATG CAGAAGCTGG 
GGGCTCCCCA AAGCTCAGTC AGGCTCTGTC TTATCTGTCC ACTCGCAGCC AGCAGACACC 
AGCTACATCC TGCAGCTTCC CAAAGACACA CCTCACCTGC TCCAGCCCCA CAGCGCTCCC 
ACCTGGGGCA CCATTCCCAA ACTGCCCCCA CCAGGCCGCT CCCCCTTGGC TCAGAGGCCA 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAT TCTCTGGACG TTCAGGGCCT GGGCAGCCGG 
GAAGACCTGC TGTCCGAGGT GAGTGGGCCC TCCCCACCTC TGGCCCGGGC CTCCTCCTTC 
TGGGGCCACT TGGGCGCCCA GATGCAGCAG CATCCCCACC GCCAGAGCAA GATCTCGAAG 
CACATGCCCC CGCCAGCCCC CCGCCCAGGC TCAGAAACCA CCTGGAGCAA AGACCCACCC 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
ACCGTGGGCA GCCAGGAGGA GCCCCCGTCC CCACGGGACC TGAAGAAGTG TCACAGCGTG 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGCG TCCTGGCTGG ATGAACAGAG GAGACACTCC 
ATCGCGGTCA GCTGCCTGGA CGGCGGCTCC CAACCCAGCC TGGGCCCCGG CCCCTCTAGC 
CTTGGCGGCC AGCCTCTTGG GGGACCTGGA AGCCGGCCCA AGAAAAAACT CAGCCCACCT 
AGTATCTCCA TAGACCCCCC AGAGAGTCAG GGCCCTCGGC CCCCACCCAG CCCCGGCGTC 
TGCCTCCGGC GGAGGGCTCC GTCCAGCGAC TCCAGGGACC CCTCGGCCTC CGGCCCCCCT 
GACAGCATGG CTGCCTCGCC CCCCCCAAAG AAAGACGTGC TGAGTCTCTC TGGTTTAGCC 
TCTGACCCAG CAGACCTGGA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624647.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624704.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X1, mRNA.

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CloneID	OCe11910
Clone ID Related Accession (Same CDS sequence)	XM_005624647.3 , XM_005624647.2
Accession Version	XM_005624647.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624647.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGAGGCCCA GTGTAAACCC 
TACTACTCAG ACTACTCCCG ATTCCGGCTC CTCGTCCACC ACTTGTGTAC CAGCCACTAT 
CTGGACCTCT TCATCACGGG TGTCATTGGT CTGAATGTGG TCACCATGGC CATGGAGCAC 
TACCAGCAGC CCCAGATCCT GGACGAGGCC TTGAAGATCT GCAACTATAT CTTCACCGTC 
ATCTTTGTCT TGGAGTCAGT TTTTAAACTC GTGGCCTTTG GCTTCCGTCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCCATC GTGCTGCTGT CCATCATGGG CATCACGCTG 
GAGGAGATTG AGGTCAATGC CTCGCTGCCC ATCAACCCCA CCATCATCCG CATCATGAGA 
GTGCTGCGCA TTGCCCGAGT GCTGAAGCTG CTGAAGATGG CTGTGGGCAT GCGGGCGCTG 
CTGGACACAG TCATGCAGGC CCTGCCTCAG GTGGGGAACC TGGGACTTCT CTTCATGTTG 
TTGTTTTTCA TCTTTGCAGC TCTGGGTGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACGCACCCCT GCGAGGGCCT GGGCCGGCAT GCCACCTTTC GGAACTTTGG CATGGCCTTC 
CTGACCCTCT TCCGAGTGTC CACAGGTGAC AACTGGAACG GCATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACATGCTAC AATACAGTCA TCTCCCCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TGATCGCTGT GCTGATGAAG 
CACCTGGAGG AAAGCAACAA GGAGGCCAAA GAGGAGGCCG AGCTGGAGGC TGAGCTGGAG 
CTGGAGATGA AGACGCTCAG CCCACAGCCC CACTCGCCAC TGGGCAGCCC CTTCCTCTGG 
CCGGGGGTCG AGGGCCCCGA CAGCCCCGAT AGCCCCAAGC TCAGGGCCCC GCACACCGCG 
GCCCACACGG GAGCAGCCTC GCGCTTCTCC CTGGAACACC CCACGGACAG ACAGCTGTTT 
GACACCATAT CCCTGCTGAT CCAGGGCTCC CTGGAAGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTCCCG CCCGCAGCCT GGGCGGCTCC 
GACCCACAGA TCCCTCTAGC TGAGATGGAG GCTCTGTCTC TGACGTCAGA GATTGTGTCT 
GAACCGTCCC ACTCTCTAGC TCTGACGGAT GACTCTTTGC CTGATGACAC TCACACACTC 
TTACCTAGTG CCCTGGACAG CAATATGGAG CCCCACCCCG AGGAGATGCC CCTTGCACTG 
GGACTGGACC TGCTGACTGT GAGGAAGTCT GGGGTCAGCC GGACACACTC CCTGCCCAAT 
GACAGCTACA TGTTCCGGGA CGGAGGCACC GCTGAGGGGT CCCTAGGATG CAGAAGCTGG 
GGGCTCCCCA AAGCTCAGTC AGGCTCTGTC TTATCTGTCC ACTCGCAGCC AGCAGACACC 
AGCTACATCC TGCAGCTTCC CAAAGACACA CCTCACCTGC TCCAGCCCCA CAGCGCTCCC 
ACCTGGGGCA CCATTCCCAA ACTGCCCCCA CCAGGCCGCT CCCCCTTGGC TCAGAGGCCA 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAT TCTCTGGACG TTCAGGGCCT GGGCAGCCGG 
GAAGACCTGC TGTCCGAGGT GAGTGGGCCC TCCCCACCTC TGGCCCGGGC CTCCTCCTTC 
TGGGGCCACT TGGGCGCCCA GATGCAGCAG CATCCCCACC GCCAGAGCAA GATCTCGAAG 
CACATGCCCC CGCCAGCCCC CCGCCCAGGC TCAGAAACCA CCTGGAGCAA AGACCCACCC 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
ACCGTGGGCA GCCAGGAGGA GCCCCCGTCC CCACGGGACC TGAAGAAGTG TCACAGCGTG 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGCG TCCTGGCTGG ATGAACAGAG GAGACACTCC 
ATCGCGGTCA GCTGCCTGGA CGGCGGCTCC CAACCCAGCC TGGGCCCCGG CCCCTCTAGC 
CTTGGCGGCC AGCCTCTTGG GGGACCTGGA AGCCGGCCCA AGAAAAAACT CAGCCCACCT 
AGTATCTCCA TAGACCCCCC AGAGAGTCAG GGCCCTCGGC CCCCACCCAG CCCCGGCGTC 
TGCCTCCGGC GGAGGGCTCC GTCCAGCGAC TCCAGGGACC CCTCGGCCTC CGGCCCCCCT 
GACAGCATGG CTGCCTCGCC CCCCCCAAAG AAAGACGTGC TGAGTCTCTC TGGTTTAGCC 
TCTGACCCAG CAGACCTGGA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624704.1
CDS	527..7693
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624647.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624647.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGAGGCCCA GTGTAAACCC 
TACTACTCAG ACTACTCCCG ATTCCGGCTC CTCGTCCACC ACTTGTGTAC CAGCCACTAT 
CTGGACCTCT TCATCACGGG TGTCATTGGT CTGAATGTGG TCACCATGGC CATGGAGCAC 
TACCAGCAGC CCCAGATCCT GGACGAGGCC TTGAAGATCT GCAACTATAT CTTCACCGTC 
ATCTTTGTCT TGGAGTCAGT TTTTAAACTC GTGGCCTTTG GCTTCCGTCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCCATC GTGCTGCTGT CCATCATGGG CATCACGCTG 
GAGGAGATTG AGGTCAATGC CTCGCTGCCC ATCAACCCCA CCATCATCCG CATCATGAGA 
GTGCTGCGCA TTGCCCGAGT GCTGAAGCTG CTGAAGATGG CTGTGGGCAT GCGGGCGCTG 
CTGGACACAG TCATGCAGGC CCTGCCTCAG GTGGGGAACC TGGGACTTCT CTTCATGTTG 
TTGTTTTTCA TCTTTGCAGC TCTGGGTGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACGCACCCCT GCGAGGGCCT GGGCCGGCAT GCCACCTTTC GGAACTTTGG CATGGCCTTC 
CTGACCCTCT TCCGAGTGTC CACAGGTGAC AACTGGAACG GCATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACATGCTAC AATACAGTCA TCTCCCCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TGATCGCTGT GCTGATGAAG 
CACCTGGAGG AAAGCAACAA GGAGGCCAAA GAGGAGGCCG AGCTGGAGGC TGAGCTGGAG 
CTGGAGATGA AGACGCTCAG CCCACAGCCC CACTCGCCAC TGGGCAGCCC CTTCCTCTGG 
CCGGGGGTCG AGGGCCCCGA CAGCCCCGAT AGCCCCAAGC TCAGGGCCCC GCACACCGCG 
GCCCACACGG GAGCAGCCTC GCGCTTCTCC CTGGAACACC CCACGGACAG ACAGCTGTTT 
GACACCATAT CCCTGCTGAT CCAGGGCTCC CTGGAAGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTCCCG CCCGCAGCCT GGGCGGCTCC 
GACCCACAGA TCCCTCTAGC TGAGATGGAG GCTCTGTCTC TGACGTCAGA GATTGTGTCT 
GAACCGTCCC ACTCTCTAGC TCTGACGGAT GACTCTTTGC CTGATGACAC TCACACACTC 
TTACCTAGTG CCCTGGACAG CAATATGGAG CCCCACCCCG AGGAGATGCC CCTTGCACTG 
GGACTGGACC TGCTGACTGT GAGGAAGTCT GGGGTCAGCC GGACACACTC CCTGCCCAAT 
GACAGCTACA TGTTCCGGGA CGGAGGCACC GCTGAGGGGT CCCTAGGATG CAGAAGCTGG 
GGGCTCCCCA AAGCTCAGTC AGGCTCTGTC TTATCTGTCC ACTCGCAGCC AGCAGACACC 
AGCTACATCC TGCAGCTTCC CAAAGACACA CCTCACCTGC TCCAGCCCCA CAGCGCTCCC 
ACCTGGGGCA CCATTCCCAA ACTGCCCCCA CCAGGCCGCT CCCCCTTGGC TCAGAGGCCA 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAT TCTCTGGACG TTCAGGGCCT GGGCAGCCGG 
GAAGACCTGC TGTCCGAGGT GAGTGGGCCC TCCCCACCTC TGGCCCGGGC CTCCTCCTTC 
TGGGGCCACT TGGGCGCCCA GATGCAGCAG CATCCCCACC GCCAGAGCAA GATCTCGAAG 
CACATGCCCC CGCCAGCCCC CCGCCCAGGC TCAGAAACCA CCTGGAGCAA AGACCCACCC 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
ACCGTGGGCA GCCAGGAGGA GCCCCCGTCC CCACGGGACC TGAAGAAGTG TCACAGCGTG 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGCG TCCTGGCTGG ATGAACAGAG GAGACACTCC 
ATCGCGGTCA GCTGCCTGGA CGGCGGCTCC CAACCCAGCC TGGGCCCCGG CCCCTCTAGC 
CTTGGCGGCC AGCCTCTTGG GGGACCTGGA AGCCGGCCCA AGAAAAAACT CAGCCCACCT 
AGTATCTCCA TAGACCCCCC AGAGAGTCAG GGCCCTCGGC CCCCACCCAG CCCCGGCGTC 
TGCCTCCGGC GGAGGGCTCC GTCCAGCGAC TCCAGGGACC CCTCGGCCTC CGGCCCCCCT 
GACAGCATGG CTGCCTCGCC CCCCCCAAAG AAAGACGTGC TGAGTCTCTC TGGTTTAGCC 
TCTGACCCAG CAGACCTGGA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624648.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624705.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X2, mRNA.

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CloneID	OCe11911
Clone ID Related Accession (Same CDS sequence)	XM_005624648.2
Accession Version	XM_005624648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGATCT AATGCTGGAC 
GATGTAATTG CTTCCGGCAG CTCATCCAGC GCGGCGCCAG AGGCCCAGTG TAAACCCTAC 
TACTCAGACT ACTCCCGATT CCGGCTCCTC GTCCACCACT TGTGTACCAG CCACTATCTG 
GACCTCTTCA TCACGGGTGT CATTGGTCTG AATGTGGTCA CCATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCCTTG AAGATCTGCA ACTATATCTT CACCGTCATC 
TTTGTCTTGG AGTCAGTTTT TAAACTCGTG GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATCGTG CTGCTGTCCA TCATGGGCAT CACGCTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGAGTG 
CTGCGCATTG CCCGAGTGCT GAAGCTGCTG AAGATGGCTG TGGGCATGCG GGCGCTGCTG 
GACACAGTCA TGCAGGCCCT GCCTCAGGTG GGGAACCTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGTGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG 
ACCCTCTTCC GAGTGTCCAC AGGTGACAAC TGGAACGGCA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC ATGCTACAAT ACAGTCATCT CCCCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTGA TCGCTGTGCT GATGAAGCAC 
CTGGAGGAAA GCAACAAGGA GGCCAAAGAG GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG 
GAGATGAAGA CGCTCAGCCC ACAGCCCCAC TCGCCACTGG GCAGCCCCTT CCTCTGGCCG 
GGGGTCGAGG GCCCCGACAG CCCCGATAGC CCCAAGCTCA GGGCCCCGCA CACCGCGGCC 
CACACGGGAG CAGCCTCGCG CTTCTCCCTG GAACACCCCA CGGACAGACA GCTGTTTGAC 
ACCATATCCC TGCTGATCCA GGGCTCCCTG GAAGGGGAGC TGAAGCTGAT GGACGAGCTG 
GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCCGCCC GCAGCCTGGG CGGCTCCGAC 
CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTCTGA CGTCAGAGAT TGTGTCTGAA 
CCGTCCCACT CTCTAGCTCT GACGGATGAC TCTTTGCCTG ATGACACTCA CACACTCTTA 
CCTAGTGCCC TGGACAGCAA TATGGAGCCC CACCCCGAGG AGATGCCCCT TGCACTGGGA 
CTGGACCTGC TGACTGTGAG GAAGTCTGGG GTCAGCCGGA CACACTCCCT GCCCAATGAC 
AGCTACATGT TCCGGGACGG AGGCACCGCT GAGGGGTCCC TAGGATGCAG AAGCTGGGGG 
CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA TCTGTCCACT CGCAGCCAGC AGACACCAGC 
TACATCCTGC AGCTTCCCAA AGACACACCT CACCTGCTCC AGCCCCACAG CGCTCCCACC 
TGGGGCACCA TTCCCAAACT GCCCCCACCA GGCCGCTCCC CCTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATAAG GACTGATTCT CTGGACGTTC AGGGCCTGGG CAGCCGGGAA 
GACCTGCTGT CCGAGGTGAG TGGGCCCTCC CCACCTCTGG CCCGGGCCTC CTCCTTCTGG 
GGCCACTTGG GCGCCCAGAT GCAGCAGCAT CCCCACCGCC AGAGCAAGAT CTCGAAGCAC 
ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA GAAACCACCT GGAGCAAAGA CCCACCCGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGACC 
GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA CGGGACCTGA AGAAGTGTCA CAGCGTGGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC TGGCTGGATG AACAGAGGAG ACACTCCATC 
GCGGTCAGCT GCCTGGACGG CGGCTCCCAA CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT 
GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC CGGCCCAAGA AAAAACTCAG CCCACCTAGT 
ATCTCCATAG ACCCCCCAGA GAGTCAGGGC CCTCGGCCCC CACCCAGCCC CGGCGTCTGC 
CTCCGGCGGA GGGCTCCGTC CAGCGACTCC AGGGACCCCT CGGCCTCCGG CCCCCCTGAC 
AGCATGGCTG CCTCGCCCCC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTAGCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624705.1
CDS	527..7690
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624648.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624648.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGATCT AATGCTGGAC 
GATGTAATTG CTTCCGGCAG CTCATCCAGC GCGGCGCCAG AGGCCCAGTG TAAACCCTAC 
TACTCAGACT ACTCCCGATT CCGGCTCCTC GTCCACCACT TGTGTACCAG CCACTATCTG 
GACCTCTTCA TCACGGGTGT CATTGGTCTG AATGTGGTCA CCATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCCTTG AAGATCTGCA ACTATATCTT CACCGTCATC 
TTTGTCTTGG AGTCAGTTTT TAAACTCGTG GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATCGTG CTGCTGTCCA TCATGGGCAT CACGCTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGAGTG 
CTGCGCATTG CCCGAGTGCT GAAGCTGCTG AAGATGGCTG TGGGCATGCG GGCGCTGCTG 
GACACAGTCA TGCAGGCCCT GCCTCAGGTG GGGAACCTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGTGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG 
ACCCTCTTCC GAGTGTCCAC AGGTGACAAC TGGAACGGCA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC ATGCTACAAT ACAGTCATCT CCCCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTGA TCGCTGTGCT GATGAAGCAC 
CTGGAGGAAA GCAACAAGGA GGCCAAAGAG GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG 
GAGATGAAGA CGCTCAGCCC ACAGCCCCAC TCGCCACTGG GCAGCCCCTT CCTCTGGCCG 
GGGGTCGAGG GCCCCGACAG CCCCGATAGC CCCAAGCTCA GGGCCCCGCA CACCGCGGCC 
CACACGGGAG CAGCCTCGCG CTTCTCCCTG GAACACCCCA CGGACAGACA GCTGTTTGAC 
ACCATATCCC TGCTGATCCA GGGCTCCCTG GAAGGGGAGC TGAAGCTGAT GGACGAGCTG 
GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCCGCCC GCAGCCTGGG CGGCTCCGAC 
CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTCTGA CGTCAGAGAT TGTGTCTGAA 
CCGTCCCACT CTCTAGCTCT GACGGATGAC TCTTTGCCTG ATGACACTCA CACACTCTTA 
CCTAGTGCCC TGGACAGCAA TATGGAGCCC CACCCCGAGG AGATGCCCCT TGCACTGGGA 
CTGGACCTGC TGACTGTGAG GAAGTCTGGG GTCAGCCGGA CACACTCCCT GCCCAATGAC 
AGCTACATGT TCCGGGACGG AGGCACCGCT GAGGGGTCCC TAGGATGCAG AAGCTGGGGG 
CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA TCTGTCCACT CGCAGCCAGC AGACACCAGC 
TACATCCTGC AGCTTCCCAA AGACACACCT CACCTGCTCC AGCCCCACAG CGCTCCCACC 
TGGGGCACCA TTCCCAAACT GCCCCCACCA GGCCGCTCCC CCTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATAAG GACTGATTCT CTGGACGTTC AGGGCCTGGG CAGCCGGGAA 
GACCTGCTGT CCGAGGTGAG TGGGCCCTCC CCACCTCTGG CCCGGGCCTC CTCCTTCTGG 
GGCCACTTGG GCGCCCAGAT GCAGCAGCAT CCCCACCGCC AGAGCAAGAT CTCGAAGCAC 
ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA GAAACCACCT GGAGCAAAGA CCCACCCGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGACC 
GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA CGGGACCTGA AGAAGTGTCA CAGCGTGGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC TGGCTGGATG AACAGAGGAG ACACTCCATC 
GCGGTCAGCT GCCTGGACGG CGGCTCCCAA CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT 
GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC CGGCCCAAGA AAAAACTCAG CCCACCTAGT 
ATCTCCATAG ACCCCCCAGA GAGTCAGGGC CCTCGGCCCC CACCCAGCCC CGGCGTCTGC 
CTCCGGCGGA GGGCTCCGTC CAGCGACTCC AGGGACCCCT CGGCCTCCGG CCCCCCTGAC 
AGCATGGCTG CCTCGCCCCC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTAGCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624649.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624706.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X3, mRNA.

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CloneID	OCe11912
Clone ID Related Accession (Same CDS sequence)	XM_005624649.2 , XM_005624649.3
Accession Version	XM_005624649.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAT CTAATGCTGG ACGATGTAAT TGCTTCCGGC 
AGCTCATCCA GCGCGGCGCC AGAGGCCCAG TGTAAACCCT ACTACTCAGA CTACTCCCGA 
TTCCGGCTCC TCGTCCACCA CTTGTGTACC AGCCACTATC TGGACCTCTT CATCACGGGT 
GTCATTGGTC TGAATGTGGT CACCATGGCC ATGGAGCACT ACCAGCAGCC CCAGATCCTG 
GACGAGGCCT TGAAGATCTG CAACTATATC TTCACCGTCA TCTTTGTCTT GGAGTCAGTT 
TTTAAACTCG TGGCCTTTGG CTTCCGTCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTGCTGTC CATCATGGGC ATCACGCTGG AGGAGATTGA GGTCAATGCC 
TCGCTGCCCA TCAACCCCAC CATCATCCGC ATCATGAGAG TGCTGCGCAT TGCCCGAGTG 
CTGAAGCTGC TGAAGATGGC TGTGGGCATG CGGGCGCTGC TGGACACAGT CATGCAGGCC 
CTGCCTCAGG TGGGGAACCT GGGACTTCTC TTCATGTTGT TGTTTTTCAT CTTTGCAGCT 
CTGGGTGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CGCACCCCTG CGAGGGCCTG 
GGCCGGCATG CCACCTTTCG GAACTTTGGC ATGGCCTTCC TGACCCTCTT CCGAGTGTCC 
ACAGGTGACA ACTGGAACGG CATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACATGCTACA ATACAGTCAT CTCCCCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT GATCGCTGTG CTGATGAAGC ACCTGGAGGA AAGCAACAAG 
GAGGCCAAAG AGGAGGCCGA GCTGGAGGCT GAGCTGGAGC TGGAGATGAA GACGCTCAGC 
CCACAGCCCC ACTCGCCACT GGGCAGCCCC TTCCTCTGGC CGGGGGTCGA GGGCCCCGAC 
AGCCCCGATA GCCCCAAGCT CAGGGCCCCG CACACCGCGG CCCACACGGG AGCAGCCTCG 
CGCTTCTCCC TGGAACACCC CACGGACAGA CAGCTGTTTG ACACCATATC CCTGCTGATC 
CAGGGCTCCC TGGAAGGGGA GCTGAAGCTG ATGGACGAGC TGGCAGGCCC AGGGGGCCAG 
CCCTCTGCCT TCCCTCCCGC CCGCAGCCTG GGCGGCTCCG ACCCACAGAT CCCTCTAGCT 
GAGATGGAGG CTCTGTCTCT GACGTCAGAG ATTGTGTCTG AACCGTCCCA CTCTCTAGCT 
CTGACGGATG ACTCTTTGCC TGATGACACT CACACACTCT TACCTAGTGC CCTGGACAGC 
AATATGGAGC CCCACCCCGA GGAGATGCCC CTTGCACTGG GACTGGACCT GCTGACTGTG 
AGGAAGTCTG GGGTCAGCCG GACACACTCC CTGCCCAATG ACAGCTACAT GTTCCGGGAC 
GGAGGCACCG CTGAGGGGTC CCTAGGATGC AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA 
GGCTCTGTCT TATCTGTCCA CTCGCAGCCA GCAGACACCA GCTACATCCT GCAGCTTCCC 
AAAGACACAC CTCACCTGCT CCAGCCCCAC AGCGCTCCCA CCTGGGGCAC CATTCCCAAA 
CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT CAGAGGCCAC TCAGGCGCCA GGCAGCAATA 
AGGACTGATT CTCTGGACGT TCAGGGCCTG GGCAGCCGGG AAGACCTGCT GTCCGAGGTG 
AGTGGGCCCT CCCCACCTCT GGCCCGGGCC TCCTCCTTCT GGGGCCACTT GGGCGCCCAG 
ATGCAGCAGC ATCCCCACCG CCAGAGCAAG ATCTCGAAGC ACATGCCCCC GCCAGCCCCC 
CGCCCAGGCT CAGAAACCAC CTGGAGCAAA GACCCACCCG AGACCAGAAG CAGCTTAGAG 
CTGGACACGG AGCTGAGCTG GATTTCAGGA GACCTCCTGA CCGTGGGCAG CCAGGAGGAG 
CCCCCGTCCC CACGGGACCT GAAGAAGTGT CACAGCGTGG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGCGT CCTGGCTGGA TGAACAGAGG AGACACTCCA TCGCGGTCAG CTGCCTGGAC 
GGCGGCTCCC AACCCAGCCT GGGCCCCGGC CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG 
GGACCTGGAA GCCGGCCCAA GAAAAAACTC AGCCCACCTA GTATCTCCAT AGACCCCCCA 
GAGAGTCAGG GCCCTCGGCC CCCACCCAGC CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG 
TCCAGCGACT CCAGGGACCC CTCGGCCTCC GGCCCCCCTG ACAGCATGGC TGCCTCGCCC 
CCCCCAAAGA AAGACGTGCT GAGTCTCTCT GGTTTAGCCT CTGACCCAGC AGACCTGGAC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624706.1
CDS	527..7672
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624649.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624649.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAT CTAATGCTGG ACGATGTAAT TGCTTCCGGC 
AGCTCATCCA GCGCGGCGCC AGAGGCCCAG TGTAAACCCT ACTACTCAGA CTACTCCCGA 
TTCCGGCTCC TCGTCCACCA CTTGTGTACC AGCCACTATC TGGACCTCTT CATCACGGGT 
GTCATTGGTC TGAATGTGGT CACCATGGCC ATGGAGCACT ACCAGCAGCC CCAGATCCTG 
GACGAGGCCT TGAAGATCTG CAACTATATC TTCACCGTCA TCTTTGTCTT GGAGTCAGTT 
TTTAAACTCG TGGCCTTTGG CTTCCGTCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTGCTGTC CATCATGGGC ATCACGCTGG AGGAGATTGA GGTCAATGCC 
TCGCTGCCCA TCAACCCCAC CATCATCCGC ATCATGAGAG TGCTGCGCAT TGCCCGAGTG 
CTGAAGCTGC TGAAGATGGC TGTGGGCATG CGGGCGCTGC TGGACACAGT CATGCAGGCC 
CTGCCTCAGG TGGGGAACCT GGGACTTCTC TTCATGTTGT TGTTTTTCAT CTTTGCAGCT 
CTGGGTGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CGCACCCCTG CGAGGGCCTG 
GGCCGGCATG CCACCTTTCG GAACTTTGGC ATGGCCTTCC TGACCCTCTT CCGAGTGTCC 
ACAGGTGACA ACTGGAACGG CATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACATGCTACA ATACAGTCAT CTCCCCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT GATCGCTGTG CTGATGAAGC ACCTGGAGGA AAGCAACAAG 
GAGGCCAAAG AGGAGGCCGA GCTGGAGGCT GAGCTGGAGC TGGAGATGAA GACGCTCAGC 
CCACAGCCCC ACTCGCCACT GGGCAGCCCC TTCCTCTGGC CGGGGGTCGA GGGCCCCGAC 
AGCCCCGATA GCCCCAAGCT CAGGGCCCCG CACACCGCGG CCCACACGGG AGCAGCCTCG 
CGCTTCTCCC TGGAACACCC CACGGACAGA CAGCTGTTTG ACACCATATC CCTGCTGATC 
CAGGGCTCCC TGGAAGGGGA GCTGAAGCTG ATGGACGAGC TGGCAGGCCC AGGGGGCCAG 
CCCTCTGCCT TCCCTCCCGC CCGCAGCCTG GGCGGCTCCG ACCCACAGAT CCCTCTAGCT 
GAGATGGAGG CTCTGTCTCT GACGTCAGAG ATTGTGTCTG AACCGTCCCA CTCTCTAGCT 
CTGACGGATG ACTCTTTGCC TGATGACACT CACACACTCT TACCTAGTGC CCTGGACAGC 
AATATGGAGC CCCACCCCGA GGAGATGCCC CTTGCACTGG GACTGGACCT GCTGACTGTG 
AGGAAGTCTG GGGTCAGCCG GACACACTCC CTGCCCAATG ACAGCTACAT GTTCCGGGAC 
GGAGGCACCG CTGAGGGGTC CCTAGGATGC AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA 
GGCTCTGTCT TATCTGTCCA CTCGCAGCCA GCAGACACCA GCTACATCCT GCAGCTTCCC 
AAAGACACAC CTCACCTGCT CCAGCCCCAC AGCGCTCCCA CCTGGGGCAC CATTCCCAAA 
CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT CAGAGGCCAC TCAGGCGCCA GGCAGCAATA 
AGGACTGATT CTCTGGACGT TCAGGGCCTG GGCAGCCGGG AAGACCTGCT GTCCGAGGTG 
AGTGGGCCCT CCCCACCTCT GGCCCGGGCC TCCTCCTTCT GGGGCCACTT GGGCGCCCAG 
ATGCAGCAGC ATCCCCACCG CCAGAGCAAG ATCTCGAAGC ACATGCCCCC GCCAGCCCCC 
CGCCCAGGCT CAGAAACCAC CTGGAGCAAA GACCCACCCG AGACCAGAAG CAGCTTAGAG 
CTGGACACGG AGCTGAGCTG GATTTCAGGA GACCTCCTGA CCGTGGGCAG CCAGGAGGAG 
CCCCCGTCCC CACGGGACCT GAAGAAGTGT CACAGCGTGG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGCGT CCTGGCTGGA TGAACAGAGG AGACACTCCA TCGCGGTCAG CTGCCTGGAC 
GGCGGCTCCC AACCCAGCCT GGGCCCCGGC CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG 
GGACCTGGAA GCCGGCCCAA GAAAAAACTC AGCCCACCTA GTATCTCCAT AGACCCCCCA 
GAGAGTCAGG GCCCTCGGCC CCCACCCAGC CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG 
TCCAGCGACT CCAGGGACCC CTCGGCCTCC GGCCCCCCTG ACAGCATGGC TGCCTCGCCC 
CCCCCAAAGA AAGACGTGCT GAGTCTCTCT GGTTTAGCCT CTGACCCAGC AGACCTGGAC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624649.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624706.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X3, mRNA.

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CloneID	OCe11912
Clone ID Related Accession (Same CDS sequence)	XM_005624649.2 , XM_005624649.3
Accession Version	XM_005624649.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAT CTAATGCTGG ACGATGTAAT TGCTTCCGGC 
AGCTCATCCA GCGCGGCGCC AGAGGCCCAG TGTAAACCCT ACTACTCAGA CTACTCCCGA 
TTCCGGCTCC TCGTCCACCA CTTGTGTACC AGCCACTATC TGGACCTCTT CATCACGGGT 
GTCATTGGTC TGAATGTGGT CACCATGGCC ATGGAGCACT ACCAGCAGCC CCAGATCCTG 
GACGAGGCCT TGAAGATCTG CAACTATATC TTCACCGTCA TCTTTGTCTT GGAGTCAGTT 
TTTAAACTCG TGGCCTTTGG CTTCCGTCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTGCTGTC CATCATGGGC ATCACGCTGG AGGAGATTGA GGTCAATGCC 
TCGCTGCCCA TCAACCCCAC CATCATCCGC ATCATGAGAG TGCTGCGCAT TGCCCGAGTG 
CTGAAGCTGC TGAAGATGGC TGTGGGCATG CGGGCGCTGC TGGACACAGT CATGCAGGCC 
CTGCCTCAGG TGGGGAACCT GGGACTTCTC TTCATGTTGT TGTTTTTCAT CTTTGCAGCT 
CTGGGTGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CGCACCCCTG CGAGGGCCTG 
GGCCGGCATG CCACCTTTCG GAACTTTGGC ATGGCCTTCC TGACCCTCTT CCGAGTGTCC 
ACAGGTGACA ACTGGAACGG CATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACATGCTACA ATACAGTCAT CTCCCCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT GATCGCTGTG CTGATGAAGC ACCTGGAGGA AAGCAACAAG 
GAGGCCAAAG AGGAGGCCGA GCTGGAGGCT GAGCTGGAGC TGGAGATGAA GACGCTCAGC 
CCACAGCCCC ACTCGCCACT GGGCAGCCCC TTCCTCTGGC CGGGGGTCGA GGGCCCCGAC 
AGCCCCGATA GCCCCAAGCT CAGGGCCCCG CACACCGCGG CCCACACGGG AGCAGCCTCG 
CGCTTCTCCC TGGAACACCC CACGGACAGA CAGCTGTTTG ACACCATATC CCTGCTGATC 
CAGGGCTCCC TGGAAGGGGA GCTGAAGCTG ATGGACGAGC TGGCAGGCCC AGGGGGCCAG 
CCCTCTGCCT TCCCTCCCGC CCGCAGCCTG GGCGGCTCCG ACCCACAGAT CCCTCTAGCT 
GAGATGGAGG CTCTGTCTCT GACGTCAGAG ATTGTGTCTG AACCGTCCCA CTCTCTAGCT 
CTGACGGATG ACTCTTTGCC TGATGACACT CACACACTCT TACCTAGTGC CCTGGACAGC 
AATATGGAGC CCCACCCCGA GGAGATGCCC CTTGCACTGG GACTGGACCT GCTGACTGTG 
AGGAAGTCTG GGGTCAGCCG GACACACTCC CTGCCCAATG ACAGCTACAT GTTCCGGGAC 
GGAGGCACCG CTGAGGGGTC CCTAGGATGC AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA 
GGCTCTGTCT TATCTGTCCA CTCGCAGCCA GCAGACACCA GCTACATCCT GCAGCTTCCC 
AAAGACACAC CTCACCTGCT CCAGCCCCAC AGCGCTCCCA CCTGGGGCAC CATTCCCAAA 
CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT CAGAGGCCAC TCAGGCGCCA GGCAGCAATA 
AGGACTGATT CTCTGGACGT TCAGGGCCTG GGCAGCCGGG AAGACCTGCT GTCCGAGGTG 
AGTGGGCCCT CCCCACCTCT GGCCCGGGCC TCCTCCTTCT GGGGCCACTT GGGCGCCCAG 
ATGCAGCAGC ATCCCCACCG CCAGAGCAAG ATCTCGAAGC ACATGCCCCC GCCAGCCCCC 
CGCCCAGGCT CAGAAACCAC CTGGAGCAAA GACCCACCCG AGACCAGAAG CAGCTTAGAG 
CTGGACACGG AGCTGAGCTG GATTTCAGGA GACCTCCTGA CCGTGGGCAG CCAGGAGGAG 
CCCCCGTCCC CACGGGACCT GAAGAAGTGT CACAGCGTGG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGCGT CCTGGCTGGA TGAACAGAGG AGACACTCCA TCGCGGTCAG CTGCCTGGAC 
GGCGGCTCCC AACCCAGCCT GGGCCCCGGC CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG 
GGACCTGGAA GCCGGCCCAA GAAAAAACTC AGCCCACCTA GTATCTCCAT AGACCCCCCA 
GAGAGTCAGG GCCCTCGGCC CCCACCCAGC CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG 
TCCAGCGACT CCAGGGACCC CTCGGCCTCC GGCCCCCCTG ACAGCATGGC TGCCTCGCCC 
CCCCCAAAGA AAGACGTGCT GAGTCTCTCT GGTTTAGCCT CTGACCCAGC AGACCTGGAC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624706.1
CDS	505..7650
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624649.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624649.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAT CTAATGCTGG ACGATGTAAT TGCTTCCGGC 
AGCTCATCCA GCGCGGCGCC AGAGGCCCAG TGTAAACCCT ACTACTCAGA CTACTCCCGA 
TTCCGGCTCC TCGTCCACCA CTTGTGTACC AGCCACTATC TGGACCTCTT CATCACGGGT 
GTCATTGGTC TGAATGTGGT CACCATGGCC ATGGAGCACT ACCAGCAGCC CCAGATCCTG 
GACGAGGCCT TGAAGATCTG CAACTATATC TTCACCGTCA TCTTTGTCTT GGAGTCAGTT 
TTTAAACTCG TGGCCTTTGG CTTCCGTCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTGCTGTC CATCATGGGC ATCACGCTGG AGGAGATTGA GGTCAATGCC 
TCGCTGCCCA TCAACCCCAC CATCATCCGC ATCATGAGAG TGCTGCGCAT TGCCCGAGTG 
CTGAAGCTGC TGAAGATGGC TGTGGGCATG CGGGCGCTGC TGGACACAGT CATGCAGGCC 
CTGCCTCAGG TGGGGAACCT GGGACTTCTC TTCATGTTGT TGTTTTTCAT CTTTGCAGCT 
CTGGGTGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CGCACCCCTG CGAGGGCCTG 
GGCCGGCATG CCACCTTTCG GAACTTTGGC ATGGCCTTCC TGACCCTCTT CCGAGTGTCC 
ACAGGTGACA ACTGGAACGG CATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACATGCTACA ATACAGTCAT CTCCCCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT GATCGCTGTG CTGATGAAGC ACCTGGAGGA AAGCAACAAG 
GAGGCCAAAG AGGAGGCCGA GCTGGAGGCT GAGCTGGAGC TGGAGATGAA GACGCTCAGC 
CCACAGCCCC ACTCGCCACT GGGCAGCCCC TTCCTCTGGC CGGGGGTCGA GGGCCCCGAC 
AGCCCCGATA GCCCCAAGCT CAGGGCCCCG CACACCGCGG CCCACACGGG AGCAGCCTCG 
CGCTTCTCCC TGGAACACCC CACGGACAGA CAGCTGTTTG ACACCATATC CCTGCTGATC 
CAGGGCTCCC TGGAAGGGGA GCTGAAGCTG ATGGACGAGC TGGCAGGCCC AGGGGGCCAG 
CCCTCTGCCT TCCCTCCCGC CCGCAGCCTG GGCGGCTCCG ACCCACAGAT CCCTCTAGCT 
GAGATGGAGG CTCTGTCTCT GACGTCAGAG ATTGTGTCTG AACCGTCCCA CTCTCTAGCT 
CTGACGGATG ACTCTTTGCC TGATGACACT CACACACTCT TACCTAGTGC CCTGGACAGC 
AATATGGAGC CCCACCCCGA GGAGATGCCC CTTGCACTGG GACTGGACCT GCTGACTGTG 
AGGAAGTCTG GGGTCAGCCG GACACACTCC CTGCCCAATG ACAGCTACAT GTTCCGGGAC 
GGAGGCACCG CTGAGGGGTC CCTAGGATGC AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA 
GGCTCTGTCT TATCTGTCCA CTCGCAGCCA GCAGACACCA GCTACATCCT GCAGCTTCCC 
AAAGACACAC CTCACCTGCT CCAGCCCCAC AGCGCTCCCA CCTGGGGCAC CATTCCCAAA 
CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT CAGAGGCCAC TCAGGCGCCA GGCAGCAATA 
AGGACTGATT CTCTGGACGT TCAGGGCCTG GGCAGCCGGG AAGACCTGCT GTCCGAGGTG 
AGTGGGCCCT CCCCACCTCT GGCCCGGGCC TCCTCCTTCT GGGGCCACTT GGGCGCCCAG 
ATGCAGCAGC ATCCCCACCG CCAGAGCAAG ATCTCGAAGC ACATGCCCCC GCCAGCCCCC 
CGCCCAGGCT CAGAAACCAC CTGGAGCAAA GACCCACCCG AGACCAGAAG CAGCTTAGAG 
CTGGACACGG AGCTGAGCTG GATTTCAGGA GACCTCCTGA CCGTGGGCAG CCAGGAGGAG 
CCCCCGTCCC CACGGGACCT GAAGAAGTGT CACAGCGTGG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGCGT CCTGGCTGGA TGAACAGAGG AGACACTCCA TCGCGGTCAG CTGCCTGGAC 
GGCGGCTCCC AACCCAGCCT GGGCCCCGGC CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG 
GGACCTGGAA GCCGGCCCAA GAAAAAACTC AGCCCACCTA GTATCTCCAT AGACCCCCCA 
GAGAGTCAGG GCCCTCGGCC CCCACCCAGC CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG 
TCCAGCGACT CCAGGGACCC CTCGGCCTCC GGCCCCCCTG ACAGCATGGC TGCCTCGCCC 
CCCCCAAAGA AAGACGTGCT GAGTCTCTCT GGTTTAGCCT CTGACCCAGC AGACCTGGAC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_548203.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_548203.2 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X4, mRNA.

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CloneID	OCe11913
Clone ID Related Accession (Same CDS sequence)	XM_548203.6 , XM_548203.5
Accession Version	XM_548203.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_548203.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG 
GGCTCCCTGG AAGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC GGCTCCGACC CACAGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTCTGAC GTCAGAGATT GTGTCTGAAC CGTCCCACTC TCTAGCTCTG 
ACGGATGACT CTTTGCCTGA TGACACTCAC ACACTCTTAC CTAGTGCCCT GGACAGCAAT 
ATGGAGCCCC ACCCCGAGGA GATGCCCCTT GCACTGGGAC TGGACCTGCT GACTGTGAGG 
AAGTCTGGGG TCAGCCGGAC ACACTCCCTG CCCAATGACA GCTACATGTT CCGGGACGGA 
GGCACCGCTG AGGGGTCCCT AGGATGCAGA AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC 
TCTGTCTTAT CTGTCCACTC GCAGCCAGCA GACACCAGCT ACATCCTGCA GCTTCCCAAA 
GACACACCTC ACCTGCTCCA GCCCCACAGC GCTCCCACCT GGGGCACCAT TCCCAAACTG 
CCCCCACCAG GCCGCTCCCC CTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATAAGG 
ACTGATTCTC TGGACGTTCA GGGCCTGGGC AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT 
GGGCCCTCCC CACCTCTGGC CCGGGCCTCC TCCTTCTGGG GCCACTTGGG CGCCCAGATG 
CAGCAGCATC CCCACCGCCA GAGCAAGATC TCGAAGCACA TGCCCCCGCC AGCCCCCCGC 
CCAGGCTCAG AAACCACCTG GAGCAAAGAC CCACCCGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGACCG TGGGCAGCCA GGAGGAGCCC 
CCGTCCCCAC GGGACCTGAA GAAGTGTCAC AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG 
CCTGCGTCCT GGCTGGATGA ACAGAGGAGA CACTCCATCG CGGTCAGCTG CCTGGACGGC 
GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA 
CCTGGAAGCC GGCCCAAGAA AAAACTCAGC CCACCTAGTA TCTCCATAGA CCCCCCAGAG 
AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC 
AGCGACTCCA GGGACCCCTC GGCCTCCGGC CCCCCTGACA GCATGGCTGC CTCGCCCCCC 
CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTAGCCTCTG ACCCAGCAGA CCTGGACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_548203.2
CDS	505..7647
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_548203.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_548203.6

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG 
GGCTCCCTGG AAGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC GGCTCCGACC CACAGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTCTGAC GTCAGAGATT GTGTCTGAAC CGTCCCACTC TCTAGCTCTG 
ACGGATGACT CTTTGCCTGA TGACACTCAC ACACTCTTAC CTAGTGCCCT GGACAGCAAT 
ATGGAGCCCC ACCCCGAGGA GATGCCCCTT GCACTGGGAC TGGACCTGCT GACTGTGAGG 
AAGTCTGGGG TCAGCCGGAC ACACTCCCTG CCCAATGACA GCTACATGTT CCGGGACGGA 
GGCACCGCTG AGGGGTCCCT AGGATGCAGA AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC 
TCTGTCTTAT CTGTCCACTC GCAGCCAGCA GACACCAGCT ACATCCTGCA GCTTCCCAAA 
GACACACCTC ACCTGCTCCA GCCCCACAGC GCTCCCACCT GGGGCACCAT TCCCAAACTG 
CCCCCACCAG GCCGCTCCCC CTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATAAGG 
ACTGATTCTC TGGACGTTCA GGGCCTGGGC AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT 
GGGCCCTCCC CACCTCTGGC CCGGGCCTCC TCCTTCTGGG GCCACTTGGG CGCCCAGATG 
CAGCAGCATC CCCACCGCCA GAGCAAGATC TCGAAGCACA TGCCCCCGCC AGCCCCCCGC 
CCAGGCTCAG AAACCACCTG GAGCAAAGAC CCACCCGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGACCG TGGGCAGCCA GGAGGAGCCC 
CCGTCCCCAC GGGACCTGAA GAAGTGTCAC AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG 
CCTGCGTCCT GGCTGGATGA ACAGAGGAGA CACTCCATCG CGGTCAGCTG CCTGGACGGC 
GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA 
CCTGGAAGCC GGCCCAAGAA AAAACTCAGC CCACCTAGTA TCTCCATAGA CCCCCCAGAG 
AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC 
AGCGACTCCA GGGACCCCTC GGCCTCCGGC CCCCCTGACA GCATGGCTGC CTCGCCCCCC 
CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTAGCCTCTG ACCCAGCAGA CCTGGACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_548203.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_548203.2 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X4, mRNA.

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CloneID	OCe11913
Clone ID Related Accession (Same CDS sequence)	XM_548203.6 , XM_548203.5
Accession Version	XM_548203.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_548203.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG 
GGCTCCCTGG AAGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC GGCTCCGACC CACAGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTCTGAC GTCAGAGATT GTGTCTGAAC CGTCCCACTC TCTAGCTCTG 
ACGGATGACT CTTTGCCTGA TGACACTCAC ACACTCTTAC CTAGTGCCCT GGACAGCAAT 
ATGGAGCCCC ACCCCGAGGA GATGCCCCTT GCACTGGGAC TGGACCTGCT GACTGTGAGG 
AAGTCTGGGG TCAGCCGGAC ACACTCCCTG CCCAATGACA GCTACATGTT CCGGGACGGA 
GGCACCGCTG AGGGGTCCCT AGGATGCAGA AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC 
TCTGTCTTAT CTGTCCACTC GCAGCCAGCA GACACCAGCT ACATCCTGCA GCTTCCCAAA 
GACACACCTC ACCTGCTCCA GCCCCACAGC GCTCCCACCT GGGGCACCAT TCCCAAACTG 
CCCCCACCAG GCCGCTCCCC CTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATAAGG 
ACTGATTCTC TGGACGTTCA GGGCCTGGGC AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT 
GGGCCCTCCC CACCTCTGGC CCGGGCCTCC TCCTTCTGGG GCCACTTGGG CGCCCAGATG 
CAGCAGCATC CCCACCGCCA GAGCAAGATC TCGAAGCACA TGCCCCCGCC AGCCCCCCGC 
CCAGGCTCAG AAACCACCTG GAGCAAAGAC CCACCCGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGACCG TGGGCAGCCA GGAGGAGCCC 
CCGTCCCCAC GGGACCTGAA GAAGTGTCAC AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG 
CCTGCGTCCT GGCTGGATGA ACAGAGGAGA CACTCCATCG CGGTCAGCTG CCTGGACGGC 
GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA 
CCTGGAAGCC GGCCCAAGAA AAAACTCAGC CCACCTAGTA TCTCCATAGA CCCCCCAGAG 
AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC 
AGCGACTCCA GGGACCCCTC GGCCTCCGGC CCCCCTGACA GCATGGCTGC CTCGCCCCCC 
CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTAGCCTCTG ACCCAGCAGA CCTGGACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_548203.2
CDS	527..7669
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_548203.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_548203.5

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG 
GGCTCCCTGG AAGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC GGCTCCGACC CACAGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTCTGAC GTCAGAGATT GTGTCTGAAC CGTCCCACTC TCTAGCTCTG 
ACGGATGACT CTTTGCCTGA TGACACTCAC ACACTCTTAC CTAGTGCCCT GGACAGCAAT 
ATGGAGCCCC ACCCCGAGGA GATGCCCCTT GCACTGGGAC TGGACCTGCT GACTGTGAGG 
AAGTCTGGGG TCAGCCGGAC ACACTCCCTG CCCAATGACA GCTACATGTT CCGGGACGGA 
GGCACCGCTG AGGGGTCCCT AGGATGCAGA AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC 
TCTGTCTTAT CTGTCCACTC GCAGCCAGCA GACACCAGCT ACATCCTGCA GCTTCCCAAA 
GACACACCTC ACCTGCTCCA GCCCCACAGC GCTCCCACCT GGGGCACCAT TCCCAAACTG 
CCCCCACCAG GCCGCTCCCC CTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATAAGG 
ACTGATTCTC TGGACGTTCA GGGCCTGGGC AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT 
GGGCCCTCCC CACCTCTGGC CCGGGCCTCC TCCTTCTGGG GCCACTTGGG CGCCCAGATG 
CAGCAGCATC CCCACCGCCA GAGCAAGATC TCGAAGCACA TGCCCCCGCC AGCCCCCCGC 
CCAGGCTCAG AAACCACCTG GAGCAAAGAC CCACCCGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGACCG TGGGCAGCCA GGAGGAGCCC 
CCGTCCCCAC GGGACCTGAA GAAGTGTCAC AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG 
CCTGCGTCCT GGCTGGATGA ACAGAGGAGA CACTCCATCG CGGTCAGCTG CCTGGACGGC 
GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA 
CCTGGAAGCC GGCCCAAGAA AAAACTCAGC CCACCTAGTA TCTCCATAGA CCCCCCAGAG 
AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC 
AGCGACTCCA GGGACCCCTC GGCCTCCGGC CCCCCTGACA GCATGGCTGC CTCGCCCCCC 
CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTAGCCTCTG ACCCAGCAGA CCTGGACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624650.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624707.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X5, mRNA.

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CloneID	OCe11914
Clone ID Related Accession (Same CDS sequence)	XM_005624650.2
Accession Version	XM_005624650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
TTCTCAGAGA CCAAGCAGCG GGAAAGCCAG CTGATGCGAG AGCAACGTGT GCGGTTCCTG 
TCCAACGCCA GCACCCTGGC CAGCTTCTCT GAGCCGGGCA GCTGCTACGA GGAGCTGCTC 
AAGTACCTGG TGTACATCCT CCGTAAAGCG GCCCGCAGGC TGGCCCAGGT CTCCCGGGCG 
GCGGGCGTGC GGGTCGGCCT GCTGAGCAGC CCAGTGCCCC GTGGAGGCCA GGAGCCCCAG 
TCTGGCGGCA GCTGCTCTCG CTCCCACCGC CGTCCATCCG TCCACCACCT GGTGCACCAC 
CACCACCACC ATCACCACCA CTACCATCTG GGCAATGGGA CGCTGAGGGC CCCCCGGGCC 
AGCCCGGAGA TTCAGGACAA GGATGCCAGT GGGTCCCGCC GGCTCATGCT GCCACCACCC 
TCGACGCCAG CCCTCTCTGG GGGCCCTCCA GGGGGTGCAG AATCTGTGCA CAGCTTCTAC 
CATGCCGACT GCCACCTGGA GCCGGTCCGC TGCCAGGCAC CCCCTCCCAG GTCACCATCT 
GAGGCGTCGG GCAGGACTGT GGGCAGCGGG AAGGTGTACC CCACTGTGCA CACCAGCCCT 
CCACCAGAGA TGCTGAAGGA GAAAGCGCTA GTGGAGGTGG CCCCCACTTC TGGACCCCCC 
ACCCTCACCA GCCTCAACAT CCCGCCCGGG CCCTACAGCT CCATGCACAA GCTACTAGAG 
ACACAGAGCA CAGGCGCCTG CCAAAGCTCT TGCAAGATCT CCAGCCCTTG CCTGAAGGCA 
GACAGTGGAG CCGGTGGCCC AGACAGCTGC CCCTACTGTG CCCGGGCGGG GGCAGGGCAG 
GTGGAGCTCG CCGGCCACGA GATGCCTGAC TCAGACAGCG AGGCAGTTTA TGAGCTCACA 
CAAGACGTCC ACCACGGTGA TCTCCGGGAC CCCCACAGCC GGCACCGCCA GAGCCTGGGG 
CTGGACGTGG AGCCCAACTC TGTGCTGGCC TTCTGGAGGC TGATCTGTGA CACTTTCCGG 
AAGATTGTGG ACAGCAAGTA CTTTGGTCGG GGAATCATGA TTGCTATCCT GGTCAACACG 
CTCAGCATGG GCATCGAGTA CCATGAACAG CCCGAGGAGC TGACCAACGC CCTAGAAATT 
AGCAACATCG TCTTCACCAG CCTCTTTGCC CTGGAGATGC TGCTGAAGCT GCTGGTGTAC 
GGTCCCTTTG GCTACATCAA GAACCCCTAC AATATCTTCG ATGGCGTCAT TGTTGTCATC 
AGCGTGTGGG AGATCGTGGG CCAGCAGGGG GGTGGCCTGT CGGTGCTGCG GACCTTCCGC 
CTGATGCGGG TGCTGAAGCT GGTGCGCTTC CTGCCGGCAC TGCAGCGGCA GCTCGTGGTG 
CTCATGAAGA CCATGGACAA CGTGGCCACC TTCTGCATGC TACTCATGCT CTTCATCTTC 
ATCTTCAGCA TCCTGGGCAT GCATCTCTTT GGCTGCAAAT TTGCATCTGA GCGGGACGGG 
GACACGCTGC CGGACCGGAA GAATTTTGAC TCCTTGCTCT GGGCCATCGT CACCGTCTTT 
CAGATCCTGA CCCAGGAGGA CTGGAACAAG GTGCTCTACA ATGGAATGGC CTCCACATCA 
TCCTGGGCCG CCCTTTATTT CATTGCCCTC ATGACCTTTG GCAACTACGT GCTCTTTAAT 
CTGCTTGTCG CCATTCTGGT GGAGGGCTTC CAGGCGGAGG AAATCAGCAA ACGGGAAGAT 
GCGAGTGGAC AGTTAAGCTG TATTCAGCTG CCTGTCGACT CCCAGGGGGG AGATGCCACC 
AAGTCCGAAT CAGAGCCAGA TTTCTTCTCA CCCAGCCTGG AGGGCAATGG GGACAGGAAG 
AAGCGCTTGG CCTCAGTGGT GTCCCTGGGA GAGCACCCAG AGCTGCGGAG GAACCTGCTG 
CCACCTCTTA TCATCCACAC GGCGGCCACA CCCATGTCGC TCCCCAAGAG TTCCAGCACT 
GGCCTGGCCG AGGTGCTGGG CCCTGCCTCC CGCCGCACCA GCAGCAGCGG GTCTGCTGAG 
CCAGGGGCGG CCCATGAGAT GAAGTCACCG CCGAGCGCCC GCAGCACTCC GCACAGCCCC 
TGGAGTGCAG CAAGCAGCTG GACCAGCAGG CGCTCCAGCC GGAACAGCCT GGGCCGAGCC 
CCCAGCCTGA AGCGGAGGAG CCCGAGTGGG GAGCGGCGGT CCCTGTTGTC CGGCGAGGGT 
CGGGAGAGCC AGGATGAGGA GGAGAGCTCT GAAGAGGAGC GGGCCAGCCC AGCGGGCAGC 
GACCGGCGCC ATGGAGGCTC CCTGGAGCGG GAGGCCAAGA GCTCCTTCGA CCTGCCGGAC 
ACGCTGCAAG TGCCCGGACT GCACCGCACG GCCAGTGGCC GCAGCTCAGC CTCTGAGCAC 
CAGGACTGCA ACGGCAAGTC GGCCTCGGGG CGCCTGGCCC GGGCCCTGCG GCCCGACGAC 
CCCCCACTGG ATGGGGATGA TGGCGATGAC GAGGGCAACC TGAGCAGAGG GGAGCGGATA 
AGAGTGTGGA TCCGAGCCCG GCTGCCTGCC TGCTGCCGCG AGCGTGATTC CTGGTCCGCG 
TACATCTTCC CTCCTCAGTC CAGGTTCCGC CTCTTATGTC ACCGAATCAT CACCCACAAG 
ATGTTTGACC ATGTGGTCCT TGTCATCATC TTCCTCAACT GCATCACCAT CGCCATGGAG 
CGTCCCAAGA TCGACCCCCA CAGTGCTGAG CGCATCTTCC TGACCCTCTC CAATTACATC 
TTTACCGCAG TCTTTCTGGC AGAGATGACC GTGAAGGTGG TAGCGCTGGG CTGGTGCTTC 
GGGGAGCAGG CGTACCTGCG TAGCAGCTGG AACGTTCTCG ACGGGCTGCT GGTGCTCATC 
TCCATCATCG ACATCCTGGT GTCCATGGTC TCCGACAGCG GCACCAAGAT CCTGGGCATG 
TTGAGGGTGC TGCGGCTGCT ACGGACCCTG CGCCCACTCA GGGTTATTAG CAGAGCCCAG 
GGGCTGAAGC TGGTAGTGGA AACGCTGATG TCCTCACTGA AGCCCATCGG CAACATCGTG 
GTCATTTGTT GTGCCTTCTT CATCATATTT GGCATCCTGG GGGTGCAGCT CTTCAAAGGG 
AAGTTCTTCG TGTGCCAGGG CGAGGACACC AGGAACATCA CCAACAAGTC TGACTGCACC 
GAGGCCAGTT ACCGGTGGGT CCGGCACAAG TACAATTTTG ACAACCTCGG CCAGGCCCTG 
ATGTCCCTGT TCGTACTGGC CTCCAAGGAC GGCTGGGTGG ACATCATGTA CGACGGGCTG 
GACGCCGTGG GTGTGGACCA ACAGCCCATC ATGAACCACA ACCCCTGGAT GCTGCTGTAC 
TTCATCTCGT TCCTGCTCAT TGTGGCCTTC TTTGTCCTGA ACATGTTTGT GGGTGTGGTG 
GTGGAGAACT TTCACAAGTG TCGGCAGCAC CAGGAGGAGG AGGAGGCCCG GCGGCGAGAG 
GAGAAACGTC TGCGGAGACT GGAGAAAAAG AGAAGGAGTA AGGAGAAGCA GATGGCTGAT 
CTAATGCTGG ACGATGTAAT TGCTTCCGGC AGCTCATCCA GCGCGGCGCC AGAGGCCCAG 
TGTAAACCCT ACTACTCAGA CTACTCCCGA TTCCGGCTCC TCGTCCACCA CTTGTGTACC 
AGCCACTATC TGGACCTCTT CATCACGGGT GTCATTGGTC TGAATGTGGT CACCATGGCC 
ATGGAGCACT ACCAGCAGCC CCAGATCCTG GACGAGGCCT TGAAGATCTG CAACTATATC 
TTCACCGTCA TCTTTGTCTT GGAGTCAGTT TTTAAACTCG TGGCCTTTGG CTTCCGTCGG 
TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC CTGGCCATCG TGCTGCTGTC CATCATGGGC 
ATCACGCTGG AGGAGATTGA GGTCAATGCC TCGCTGCCCA TCAACCCCAC CATCATCCGC 
ATCATGAGAG TGCTGCGCAT TGCCCGAGTG CTGAAGCTGC TGAAGATGGC TGTGGGCATG 
CGGGCGCTGC TGGACACAGT CATGCAGGCC CTGCCTCAGG TGGGGAACCT GGGACTTCTC 
TTCATGTTGT TGTTTTTCAT CTTTGCAGCT CTGGGTGTGG AGCTCTTTGG AGACCTGGAG 
TGTGATGAGA CGCACCCCTG CGAGGGCCTG GGCCGGCATG CCACCTTTCG GAACTTTGGC 
ATGGCCTTCC TGACCCTCTT CCGAGTGTCC ACAGGTGACA ACTGGAACGG CATTATGAAG 
GACACCCTCC GGGACTGTGA CCAGGAGTCC ACATGCTACA ATACAGTCAT CTCCCCCATC 
TACTTCGTGT CCTTCGTGCT GACGGCCCAG TTTGTGCTGG TCAACGTGGT GATCGCTGTG 
CTGATGAAGC ACCTGGAGGA AAGCAACAAG GAGGCCAAAG AGGAGGCCGA GCTGGAGGCT 
GAGCTGGAGC TGGAGATGAA GACGCTCAGC CCACAGCCCC ACTCGCCACT GGGCAGCCCC 
TTCCTCTGGC CGGGGGTCGA GGGCCCCGAC AGCCCCGATA GCCCCAAGCT CAGGGCCCCG 
CACACCGCGG CCCACACGGG AGCAGCCTCG CGCTTCTCCC TGGAACACCC CACGGACAGA 
CAGCTGTTTG ACACCATATC CCTGCTGATC CAGGGCTCCC TGGAAGGGGA GCTGAAGCTG 
ATGGACGAGC TGGCAGGCCC AGGGGGCCAG CCCTCTGCCT TCCCTCCCGC CCGCAGCCTG 
GGCGGCTCCG ACCCACAGAT CCCTCTAGCT GAGATGGAGG CTCTGTCTCT GACGTCAGAG 
ATTGTGTCTG AACCGTCCCA CTCTCTAGCT CTGACGGATG ACTCTTTGCC TGATGACACT 
CACACACTCT TACCTAGTGC CCTGGACAGC AATATGGAGC CCCACCCCGA GGAGATGCCC 
CTTGCACTGG GACTGGACCT GCTGACTGTG AGGAAGTCTG GGGTCAGCCG GACACACTCC 
CTGCCCAATG ACAGCTACAT GTTCCGGGAC GGAGGCACCG CTGAGGGGTC CCTAGGATGC 
AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA GGCTCTGTCT TATCTGTCCA CTCGCAGCCA 
GCAGACACCA GCTACATCCT GCAGCTTCCC AAAGACACAC CTCACCTGCT CCAGCCCCAC 
AGCGCTCCCA CCTGGGGCAC CATTCCCAAA CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT 
CAGAGGCCAC TCAGGCGCCA GGCAGCAATA AGGACTGATT CTCTGGACGT TCAGGGCCTG 
GGCAGCCGGG AAGACCTGCT GTCCGAGGTG AGTGGGCCCT CCCCACCTCT GGCCCGGGCC 
TCCTCCTTCT GGGGCCACTT GGGCGCCCAG ATGCAGCAGC ATCCCCACCG CCAGAGCAAG 
ATCTCGAAGC ACATGCCCCC GCCAGCCCCC CGCCCAGGCT CAGAAACCAC CTGGAGCAAA 
GACCCACCCG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGA CCGTGGGCAG CCAGGAGGAG CCCCCGTCCC CACGGGACCT GAAGAAGTGT 
CACAGCGTGG AGGCCCAGAG CTGCCGGCGC AGGCCTGCGT CCTGGCTGGA TGAACAGAGG 
AGACACTCCA TCGCGGTCAG CTGCCTGGAC GGCGGCTCCC AACCCAGCCT GGGCCCCGGC 
CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG GGACCTGGAA GCCGGCCCAA GAAAAAACTC 
AGCCCACCTA GTATCTCCAT AGACCCCCCA GAGAGTCAGG GCCCTCGGCC CCCACCCAGC 
CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG TCCAGCGACT CCAGGGACCC CTCGGCCTCC 
GGCCCCCCTG ACAGCATGGC TGCCTCGCCC CCCCCAAAGA AAGACGTGCT GAGTCTCTCT 
GGTTTAGCCT CTGACCCAGC AGACCTGGAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624707.1
CDS	527..7642
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624650.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624650.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
TTCTCAGAGA CCAAGCAGCG GGAAAGCCAG CTGATGCGAG AGCAACGTGT GCGGTTCCTG 
TCCAACGCCA GCACCCTGGC CAGCTTCTCT GAGCCGGGCA GCTGCTACGA GGAGCTGCTC 
AAGTACCTGG TGTACATCCT CCGTAAAGCG GCCCGCAGGC TGGCCCAGGT CTCCCGGGCG 
GCGGGCGTGC GGGTCGGCCT GCTGAGCAGC CCAGTGCCCC GTGGAGGCCA GGAGCCCCAG 
TCTGGCGGCA GCTGCTCTCG CTCCCACCGC CGTCCATCCG TCCACCACCT GGTGCACCAC 
CACCACCACC ATCACCACCA CTACCATCTG GGCAATGGGA CGCTGAGGGC CCCCCGGGCC 
AGCCCGGAGA TTCAGGACAA GGATGCCAGT GGGTCCCGCC GGCTCATGCT GCCACCACCC 
TCGACGCCAG CCCTCTCTGG GGGCCCTCCA GGGGGTGCAG AATCTGTGCA CAGCTTCTAC 
CATGCCGACT GCCACCTGGA GCCGGTCCGC TGCCAGGCAC CCCCTCCCAG GTCACCATCT 
GAGGCGTCGG GCAGGACTGT GGGCAGCGGG AAGGTGTACC CCACTGTGCA CACCAGCCCT 
CCACCAGAGA TGCTGAAGGA GAAAGCGCTA GTGGAGGTGG CCCCCACTTC TGGACCCCCC 
ACCCTCACCA GCCTCAACAT CCCGCCCGGG CCCTACAGCT CCATGCACAA GCTACTAGAG 
ACACAGAGCA CAGGCGCCTG CCAAAGCTCT TGCAAGATCT CCAGCCCTTG CCTGAAGGCA 
GACAGTGGAG CCGGTGGCCC AGACAGCTGC CCCTACTGTG CCCGGGCGGG GGCAGGGCAG 
GTGGAGCTCG CCGGCCACGA GATGCCTGAC TCAGACAGCG AGGCAGTTTA TGAGCTCACA 
CAAGACGTCC ACCACGGTGA TCTCCGGGAC CCCCACAGCC GGCACCGCCA GAGCCTGGGG 
CTGGACGTGG AGCCCAACTC TGTGCTGGCC TTCTGGAGGC TGATCTGTGA CACTTTCCGG 
AAGATTGTGG ACAGCAAGTA CTTTGGTCGG GGAATCATGA TTGCTATCCT GGTCAACACG 
CTCAGCATGG GCATCGAGTA CCATGAACAG CCCGAGGAGC TGACCAACGC CCTAGAAATT 
AGCAACATCG TCTTCACCAG CCTCTTTGCC CTGGAGATGC TGCTGAAGCT GCTGGTGTAC 
GGTCCCTTTG GCTACATCAA GAACCCCTAC AATATCTTCG ATGGCGTCAT TGTTGTCATC 
AGCGTGTGGG AGATCGTGGG CCAGCAGGGG GGTGGCCTGT CGGTGCTGCG GACCTTCCGC 
CTGATGCGGG TGCTGAAGCT GGTGCGCTTC CTGCCGGCAC TGCAGCGGCA GCTCGTGGTG 
CTCATGAAGA CCATGGACAA CGTGGCCACC TTCTGCATGC TACTCATGCT CTTCATCTTC 
ATCTTCAGCA TCCTGGGCAT GCATCTCTTT GGCTGCAAAT TTGCATCTGA GCGGGACGGG 
GACACGCTGC CGGACCGGAA GAATTTTGAC TCCTTGCTCT GGGCCATCGT CACCGTCTTT 
CAGATCCTGA CCCAGGAGGA CTGGAACAAG GTGCTCTACA ATGGAATGGC CTCCACATCA 
TCCTGGGCCG CCCTTTATTT CATTGCCCTC ATGACCTTTG GCAACTACGT GCTCTTTAAT 
CTGCTTGTCG CCATTCTGGT GGAGGGCTTC CAGGCGGAGG AAATCAGCAA ACGGGAAGAT 
GCGAGTGGAC AGTTAAGCTG TATTCAGCTG CCTGTCGACT CCCAGGGGGG AGATGCCACC 
AAGTCCGAAT CAGAGCCAGA TTTCTTCTCA CCCAGCCTGG AGGGCAATGG GGACAGGAAG 
AAGCGCTTGG CCTCAGTGGT GTCCCTGGGA GAGCACCCAG AGCTGCGGAG GAACCTGCTG 
CCACCTCTTA TCATCCACAC GGCGGCCACA CCCATGTCGC TCCCCAAGAG TTCCAGCACT 
GGCCTGGCCG AGGTGCTGGG CCCTGCCTCC CGCCGCACCA GCAGCAGCGG GTCTGCTGAG 
CCAGGGGCGG CCCATGAGAT GAAGTCACCG CCGAGCGCCC GCAGCACTCC GCACAGCCCC 
TGGAGTGCAG CAAGCAGCTG GACCAGCAGG CGCTCCAGCC GGAACAGCCT GGGCCGAGCC 
CCCAGCCTGA AGCGGAGGAG CCCGAGTGGG GAGCGGCGGT CCCTGTTGTC CGGCGAGGGT 
CGGGAGAGCC AGGATGAGGA GGAGAGCTCT GAAGAGGAGC GGGCCAGCCC AGCGGGCAGC 
GACCGGCGCC ATGGAGGCTC CCTGGAGCGG GAGGCCAAGA GCTCCTTCGA CCTGCCGGAC 
ACGCTGCAAG TGCCCGGACT GCACCGCACG GCCAGTGGCC GCAGCTCAGC CTCTGAGCAC 
CAGGACTGCA ACGGCAAGTC GGCCTCGGGG CGCCTGGCCC GGGCCCTGCG GCCCGACGAC 
CCCCCACTGG ATGGGGATGA TGGCGATGAC GAGGGCAACC TGAGCAGAGG GGAGCGGATA 
AGAGTGTGGA TCCGAGCCCG GCTGCCTGCC TGCTGCCGCG AGCGTGATTC CTGGTCCGCG 
TACATCTTCC CTCCTCAGTC CAGGTTCCGC CTCTTATGTC ACCGAATCAT CACCCACAAG 
ATGTTTGACC ATGTGGTCCT TGTCATCATC TTCCTCAACT GCATCACCAT CGCCATGGAG 
CGTCCCAAGA TCGACCCCCA CAGTGCTGAG CGCATCTTCC TGACCCTCTC CAATTACATC 
TTTACCGCAG TCTTTCTGGC AGAGATGACC GTGAAGGTGG TAGCGCTGGG CTGGTGCTTC 
GGGGAGCAGG CGTACCTGCG TAGCAGCTGG AACGTTCTCG ACGGGCTGCT GGTGCTCATC 
TCCATCATCG ACATCCTGGT GTCCATGGTC TCCGACAGCG GCACCAAGAT CCTGGGCATG 
TTGAGGGTGC TGCGGCTGCT ACGGACCCTG CGCCCACTCA GGGTTATTAG CAGAGCCCAG 
GGGCTGAAGC TGGTAGTGGA AACGCTGATG TCCTCACTGA AGCCCATCGG CAACATCGTG 
GTCATTTGTT GTGCCTTCTT CATCATATTT GGCATCCTGG GGGTGCAGCT CTTCAAAGGG 
AAGTTCTTCG TGTGCCAGGG CGAGGACACC AGGAACATCA CCAACAAGTC TGACTGCACC 
GAGGCCAGTT ACCGGTGGGT CCGGCACAAG TACAATTTTG ACAACCTCGG CCAGGCCCTG 
ATGTCCCTGT TCGTACTGGC CTCCAAGGAC GGCTGGGTGG ACATCATGTA CGACGGGCTG 
GACGCCGTGG GTGTGGACCA ACAGCCCATC ATGAACCACA ACCCCTGGAT GCTGCTGTAC 
TTCATCTCGT TCCTGCTCAT TGTGGCCTTC TTTGTCCTGA ACATGTTTGT GGGTGTGGTG 
GTGGAGAACT TTCACAAGTG TCGGCAGCAC CAGGAGGAGG AGGAGGCCCG GCGGCGAGAG 
GAGAAACGTC TGCGGAGACT GGAGAAAAAG AGAAGGAGTA AGGAGAAGCA GATGGCTGAT 
CTAATGCTGG ACGATGTAAT TGCTTCCGGC AGCTCATCCA GCGCGGCGCC AGAGGCCCAG 
TGTAAACCCT ACTACTCAGA CTACTCCCGA TTCCGGCTCC TCGTCCACCA CTTGTGTACC 
AGCCACTATC TGGACCTCTT CATCACGGGT GTCATTGGTC TGAATGTGGT CACCATGGCC 
ATGGAGCACT ACCAGCAGCC CCAGATCCTG GACGAGGCCT TGAAGATCTG CAACTATATC 
TTCACCGTCA TCTTTGTCTT GGAGTCAGTT TTTAAACTCG TGGCCTTTGG CTTCCGTCGG 
TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC CTGGCCATCG TGCTGCTGTC CATCATGGGC 
ATCACGCTGG AGGAGATTGA GGTCAATGCC TCGCTGCCCA TCAACCCCAC CATCATCCGC 
ATCATGAGAG TGCTGCGCAT TGCCCGAGTG CTGAAGCTGC TGAAGATGGC TGTGGGCATG 
CGGGCGCTGC TGGACACAGT CATGCAGGCC CTGCCTCAGG TGGGGAACCT GGGACTTCTC 
TTCATGTTGT TGTTTTTCAT CTTTGCAGCT CTGGGTGTGG AGCTCTTTGG AGACCTGGAG 
TGTGATGAGA CGCACCCCTG CGAGGGCCTG GGCCGGCATG CCACCTTTCG GAACTTTGGC 
ATGGCCTTCC TGACCCTCTT CCGAGTGTCC ACAGGTGACA ACTGGAACGG CATTATGAAG 
GACACCCTCC GGGACTGTGA CCAGGAGTCC ACATGCTACA ATACAGTCAT CTCCCCCATC 
TACTTCGTGT CCTTCGTGCT GACGGCCCAG TTTGTGCTGG TCAACGTGGT GATCGCTGTG 
CTGATGAAGC ACCTGGAGGA AAGCAACAAG GAGGCCAAAG AGGAGGCCGA GCTGGAGGCT 
GAGCTGGAGC TGGAGATGAA GACGCTCAGC CCACAGCCCC ACTCGCCACT GGGCAGCCCC 
TTCCTCTGGC CGGGGGTCGA GGGCCCCGAC AGCCCCGATA GCCCCAAGCT CAGGGCCCCG 
CACACCGCGG CCCACACGGG AGCAGCCTCG CGCTTCTCCC TGGAACACCC CACGGACAGA 
CAGCTGTTTG ACACCATATC CCTGCTGATC CAGGGCTCCC TGGAAGGGGA GCTGAAGCTG 
ATGGACGAGC TGGCAGGCCC AGGGGGCCAG CCCTCTGCCT TCCCTCCCGC CCGCAGCCTG 
GGCGGCTCCG ACCCACAGAT CCCTCTAGCT GAGATGGAGG CTCTGTCTCT GACGTCAGAG 
ATTGTGTCTG AACCGTCCCA CTCTCTAGCT CTGACGGATG ACTCTTTGCC TGATGACACT 
CACACACTCT TACCTAGTGC CCTGGACAGC AATATGGAGC CCCACCCCGA GGAGATGCCC 
CTTGCACTGG GACTGGACCT GCTGACTGTG AGGAAGTCTG GGGTCAGCCG GACACACTCC 
CTGCCCAATG ACAGCTACAT GTTCCGGGAC GGAGGCACCG CTGAGGGGTC CCTAGGATGC 
AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA GGCTCTGTCT TATCTGTCCA CTCGCAGCCA 
GCAGACACCA GCTACATCCT GCAGCTTCCC AAAGACACAC CTCACCTGCT CCAGCCCCAC 
AGCGCTCCCA CCTGGGGCAC CATTCCCAAA CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT 
CAGAGGCCAC TCAGGCGCCA GGCAGCAATA AGGACTGATT CTCTGGACGT TCAGGGCCTG 
GGCAGCCGGG AAGACCTGCT GTCCGAGGTG AGTGGGCCCT CCCCACCTCT GGCCCGGGCC 
TCCTCCTTCT GGGGCCACTT GGGCGCCCAG ATGCAGCAGC ATCCCCACCG CCAGAGCAAG 
ATCTCGAAGC ACATGCCCCC GCCAGCCCCC CGCCCAGGCT CAGAAACCAC CTGGAGCAAA 
GACCCACCCG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGA CCGTGGGCAG CCAGGAGGAG CCCCCGTCCC CACGGGACCT GAAGAAGTGT 
CACAGCGTGG AGGCCCAGAG CTGCCGGCGC AGGCCTGCGT CCTGGCTGGA TGAACAGAGG 
AGACACTCCA TCGCGGTCAG CTGCCTGGAC GGCGGCTCCC AACCCAGCCT GGGCCCCGGC 
CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG GGACCTGGAA GCCGGCCCAA GAAAAAACTC 
AGCCCACCTA GTATCTCCAT AGACCCCCCA GAGAGTCAGG GCCCTCGGCC CCCACCCAGC 
CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG TCCAGCGACT CCAGGGACCC CTCGGCCTCC 
GGCCCCCCTG ACAGCATGGC TGCCTCGCCC CCCCCAAAGA AAGACGTGCT GAGTCTCTCT 
GGTTTAGCCT CTGACCCAGC AGACCTGGAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624651.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624708.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X6, mRNA.

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CloneID	OCe11915
Clone ID Related Accession (Same CDS sequence)	XM_005624651.3 , XM_005624651.2
Accession Version	XM_005624651.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624651.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA GGCCCAGTGT 
AAACCCTACT ACTCAGACTA CTCCCGATTC CGGCTCCTCG TCCACCACTT GTGTACCAGC 
CACTATCTGG ACCTCTTCAT CACGGGTGTC ATTGGTCTGA ATGTGGTCAC CATGGCCATG 
GAGCACTACC AGCAGCCCCA GATCCTGGAC GAGGCCTTGA AGATCTGCAA CTATATCTTC 
ACCGTCATCT TTGTCTTGGA GTCAGTTTTT AAACTCGTGG CCTTTGGCTT CCGTCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCCATCGTGC TGCTGTCCAT CATGGGCATC 
ACGCTGGAGG AGATTGAGGT CAATGCCTCG CTGCCCATCA ACCCCACCAT CATCCGCATC 
ATGAGAGTGC TGCGCATTGC CCGAGTGCTG AAGCTGCTGA AGATGGCTGT GGGCATGCGG 
GCGCTGCTGG ACACAGTCAT GCAGGCCCTG CCTCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGTTGTTGT TTTTCATCTT TGCAGCTCTG GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACGC ACCCCTGCGA GGGCCTGGGC CGGCATGCCA CCTTTCGGAA CTTTGGCATG 
GCCTTCCTGA CCCTCTTCCG AGTGTCCACA GGTGACAACT GGAACGGCAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACA TGCTACAATA CAGTCATCTC CCCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG 
ATGAAGCACC TGGAGGAAAG CAACAAGGAG GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG 
CTGGAGCTGG AGATGAAGAC GCTCAGCCCA CAGCCCCACT CGCCACTGGG CAGCCCCTTC 
CTCTGGCCGG GGGTCGAGGG CCCCGACAGC CCCGATAGCC CCAAGCTCAG GGCCCCGCAC 
ACCGCGGCCC ACACGGGAGC AGCCTCGCGC TTCTCCCTGG AACACCCCAC GGACAGACAG 
CTGTTTGACA CCATATCCCT GCTGATCCAG GGCTCCCTGG AAGGGGAGCT GAAGCTGATG 
GACGAGCTGG CAGGCCCAGG GGGCCAGCCC TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC 
GGCTCCGACC CACAGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTCTGAC GTCAGAGATT 
GTGTCTGAAC CGTCCCACTC TCTAGCTCTG ACGGATGACT CTTTGCCTGA TGACACTCAC 
ACACTCTTAC CTAGTGCCCT GGACAGCAAT ATGGAGCCCC ACCCCGAGGA GATGCCCCTT 
GCACTGGGAC TGGACCTGCT GACTGTGAGG AAGTCTGGGG TCAGCCGGAC ACACTCCCTG 
CCCAATGACA GCTACATGTT CCGGGACGGA GGCACCGCTG AGGGGTCCCT AGGATGCAGA 
AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC TCTGTCTTAT CTGTCCACTC GCAGCCAGCA 
GACACCAGCT ACATCCTGCA GCTTCCCAAA GACACACCTC ACCTGCTCCA GCCCCACAGC 
GCTCCCACCT GGGGCACCAT TCCCAAACTG CCCCCACCAG GCCGCTCCCC CTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATAAGG ACTGATTCTC TGGACGTTCA GGGCCTGGGC 
AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT GGGCCCTCCC CACCTCTGGC CCGGGCCTCC 
TCCTTCTGGG GCCACTTGGG CGCCCAGATG CAGCAGCATC CCCACCGCCA GAGCAAGATC 
TCGAAGCACA TGCCCCCGCC AGCCCCCCGC CCAGGCTCAG AAACCACCTG GAGCAAAGAC 
CCACCCGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGACCG TGGGCAGCCA GGAGGAGCCC CCGTCCCCAC GGGACCTGAA GAAGTGTCAC 
AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG CCTGCGTCCT GGCTGGATGA ACAGAGGAGA 
CACTCCATCG CGGTCAGCTG CCTGGACGGC GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC 
TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA CCTGGAAGCC GGCCCAAGAA AAAACTCAGC 
CCACCTAGTA TCTCCATAGA CCCCCCAGAG AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC 
GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC AGCGACTCCA GGGACCCCTC GGCCTCCGGC 
CCCCCTGACA GCATGGCTGC CTCGCCCCCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT 
TTAGCCTCTG ACCCAGCAGA CCTGGACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624708.1
CDS	505..7617
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624651.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624651.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA GGCCCAGTGT 
AAACCCTACT ACTCAGACTA CTCCCGATTC CGGCTCCTCG TCCACCACTT GTGTACCAGC 
CACTATCTGG ACCTCTTCAT CACGGGTGTC ATTGGTCTGA ATGTGGTCAC CATGGCCATG 
GAGCACTACC AGCAGCCCCA GATCCTGGAC GAGGCCTTGA AGATCTGCAA CTATATCTTC 
ACCGTCATCT TTGTCTTGGA GTCAGTTTTT AAACTCGTGG CCTTTGGCTT CCGTCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCCATCGTGC TGCTGTCCAT CATGGGCATC 
ACGCTGGAGG AGATTGAGGT CAATGCCTCG CTGCCCATCA ACCCCACCAT CATCCGCATC 
ATGAGAGTGC TGCGCATTGC CCGAGTGCTG AAGCTGCTGA AGATGGCTGT GGGCATGCGG 
GCGCTGCTGG ACACAGTCAT GCAGGCCCTG CCTCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGTTGTTGT TTTTCATCTT TGCAGCTCTG GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACGC ACCCCTGCGA GGGCCTGGGC CGGCATGCCA CCTTTCGGAA CTTTGGCATG 
GCCTTCCTGA CCCTCTTCCG AGTGTCCACA GGTGACAACT GGAACGGCAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACA TGCTACAATA CAGTCATCTC CCCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG 
ATGAAGCACC TGGAGGAAAG CAACAAGGAG GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG 
CTGGAGCTGG AGATGAAGAC GCTCAGCCCA CAGCCCCACT CGCCACTGGG CAGCCCCTTC 
CTCTGGCCGG GGGTCGAGGG CCCCGACAGC CCCGATAGCC CCAAGCTCAG GGCCCCGCAC 
ACCGCGGCCC ACACGGGAGC AGCCTCGCGC TTCTCCCTGG AACACCCCAC GGACAGACAG 
CTGTTTGACA CCATATCCCT GCTGATCCAG GGCTCCCTGG AAGGGGAGCT GAAGCTGATG 
GACGAGCTGG CAGGCCCAGG GGGCCAGCCC TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC 
GGCTCCGACC CACAGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTCTGAC GTCAGAGATT 
GTGTCTGAAC CGTCCCACTC TCTAGCTCTG ACGGATGACT CTTTGCCTGA TGACACTCAC 
ACACTCTTAC CTAGTGCCCT GGACAGCAAT ATGGAGCCCC ACCCCGAGGA GATGCCCCTT 
GCACTGGGAC TGGACCTGCT GACTGTGAGG AAGTCTGGGG TCAGCCGGAC ACACTCCCTG 
CCCAATGACA GCTACATGTT CCGGGACGGA GGCACCGCTG AGGGGTCCCT AGGATGCAGA 
AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC TCTGTCTTAT CTGTCCACTC GCAGCCAGCA 
GACACCAGCT ACATCCTGCA GCTTCCCAAA GACACACCTC ACCTGCTCCA GCCCCACAGC 
GCTCCCACCT GGGGCACCAT TCCCAAACTG CCCCCACCAG GCCGCTCCCC CTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATAAGG ACTGATTCTC TGGACGTTCA GGGCCTGGGC 
AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT GGGCCCTCCC CACCTCTGGC CCGGGCCTCC 
TCCTTCTGGG GCCACTTGGG CGCCCAGATG CAGCAGCATC CCCACCGCCA GAGCAAGATC 
TCGAAGCACA TGCCCCCGCC AGCCCCCCGC CCAGGCTCAG AAACCACCTG GAGCAAAGAC 
CCACCCGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGACCG TGGGCAGCCA GGAGGAGCCC CCGTCCCCAC GGGACCTGAA GAAGTGTCAC 
AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG CCTGCGTCCT GGCTGGATGA ACAGAGGAGA 
CACTCCATCG CGGTCAGCTG CCTGGACGGC GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC 
TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA CCTGGAAGCC GGCCCAAGAA AAAACTCAGC 
CCACCTAGTA TCTCCATAGA CCCCCCAGAG AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC 
GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC AGCGACTCCA GGGACCCCTC GGCCTCCGGC 
CCCCCTGACA GCATGGCTGC CTCGCCCCCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT 
TTAGCCTCTG ACCCAGCAGA CCTGGACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624651.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624708.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X6, mRNA.

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CloneID	OCe11915
Clone ID Related Accession (Same CDS sequence)	XM_005624651.3 , XM_005624651.2
Accession Version	XM_005624651.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA GGCCCAGTGT 
AAACCCTACT ACTCAGACTA CTCCCGATTC CGGCTCCTCG TCCACCACTT GTGTACCAGC 
CACTATCTGG ACCTCTTCAT CACGGGTGTC ATTGGTCTGA ATGTGGTCAC CATGGCCATG 
GAGCACTACC AGCAGCCCCA GATCCTGGAC GAGGCCTTGA AGATCTGCAA CTATATCTTC 
ACCGTCATCT TTGTCTTGGA GTCAGTTTTT AAACTCGTGG CCTTTGGCTT CCGTCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCCATCGTGC TGCTGTCCAT CATGGGCATC 
ACGCTGGAGG AGATTGAGGT CAATGCCTCG CTGCCCATCA ACCCCACCAT CATCCGCATC 
ATGAGAGTGC TGCGCATTGC CCGAGTGCTG AAGCTGCTGA AGATGGCTGT GGGCATGCGG 
GCGCTGCTGG ACACAGTCAT GCAGGCCCTG CCTCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGTTGTTGT TTTTCATCTT TGCAGCTCTG GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACGC ACCCCTGCGA GGGCCTGGGC CGGCATGCCA CCTTTCGGAA CTTTGGCATG 
GCCTTCCTGA CCCTCTTCCG AGTGTCCACA GGTGACAACT GGAACGGCAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACA TGCTACAATA CAGTCATCTC CCCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG 
ATGAAGCACC TGGAGGAAAG CAACAAGGAG GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG 
CTGGAGCTGG AGATGAAGAC GCTCAGCCCA CAGCCCCACT CGCCACTGGG CAGCCCCTTC 
CTCTGGCCGG GGGTCGAGGG CCCCGACAGC CCCGATAGCC CCAAGCTCAG GGCCCCGCAC 
ACCGCGGCCC ACACGGGAGC AGCCTCGCGC TTCTCCCTGG AACACCCCAC GGACAGACAG 
CTGTTTGACA CCATATCCCT GCTGATCCAG GGCTCCCTGG AAGGGGAGCT GAAGCTGATG 
GACGAGCTGG CAGGCCCAGG GGGCCAGCCC TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC 
GGCTCCGACC CACAGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTCTGAC GTCAGAGATT 
GTGTCTGAAC CGTCCCACTC TCTAGCTCTG ACGGATGACT CTTTGCCTGA TGACACTCAC 
ACACTCTTAC CTAGTGCCCT GGACAGCAAT ATGGAGCCCC ACCCCGAGGA GATGCCCCTT 
GCACTGGGAC TGGACCTGCT GACTGTGAGG AAGTCTGGGG TCAGCCGGAC ACACTCCCTG 
CCCAATGACA GCTACATGTT CCGGGACGGA GGCACCGCTG AGGGGTCCCT AGGATGCAGA 
AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC TCTGTCTTAT CTGTCCACTC GCAGCCAGCA 
GACACCAGCT ACATCCTGCA GCTTCCCAAA GACACACCTC ACCTGCTCCA GCCCCACAGC 
GCTCCCACCT GGGGCACCAT TCCCAAACTG CCCCCACCAG GCCGCTCCCC CTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATAAGG ACTGATTCTC TGGACGTTCA GGGCCTGGGC 
AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT GGGCCCTCCC CACCTCTGGC CCGGGCCTCC 
TCCTTCTGGG GCCACTTGGG CGCCCAGATG CAGCAGCATC CCCACCGCCA GAGCAAGATC 
TCGAAGCACA TGCCCCCGCC AGCCCCCCGC CCAGGCTCAG AAACCACCTG GAGCAAAGAC 
CCACCCGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGACCG TGGGCAGCCA GGAGGAGCCC CCGTCCCCAC GGGACCTGAA GAAGTGTCAC 
AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG CCTGCGTCCT GGCTGGATGA ACAGAGGAGA 
CACTCCATCG CGGTCAGCTG CCTGGACGGC GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC 
TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA CCTGGAAGCC GGCCCAAGAA AAAACTCAGC 
CCACCTAGTA TCTCCATAGA CCCCCCAGAG AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC 
GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC AGCGACTCCA GGGACCCCTC GGCCTCCGGC 
CCCCCTGACA GCATGGCTGC CTCGCCCCCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT 
TTAGCCTCTG ACCCAGCAGA CCTGGACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624708.1
CDS	527..7639
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624651.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624651.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA GGCCCAGTGT 
AAACCCTACT ACTCAGACTA CTCCCGATTC CGGCTCCTCG TCCACCACTT GTGTACCAGC 
CACTATCTGG ACCTCTTCAT CACGGGTGTC ATTGGTCTGA ATGTGGTCAC CATGGCCATG 
GAGCACTACC AGCAGCCCCA GATCCTGGAC GAGGCCTTGA AGATCTGCAA CTATATCTTC 
ACCGTCATCT TTGTCTTGGA GTCAGTTTTT AAACTCGTGG CCTTTGGCTT CCGTCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCCATCGTGC TGCTGTCCAT CATGGGCATC 
ACGCTGGAGG AGATTGAGGT CAATGCCTCG CTGCCCATCA ACCCCACCAT CATCCGCATC 
ATGAGAGTGC TGCGCATTGC CCGAGTGCTG AAGCTGCTGA AGATGGCTGT GGGCATGCGG 
GCGCTGCTGG ACACAGTCAT GCAGGCCCTG CCTCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGTTGTTGT TTTTCATCTT TGCAGCTCTG GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACGC ACCCCTGCGA GGGCCTGGGC CGGCATGCCA CCTTTCGGAA CTTTGGCATG 
GCCTTCCTGA CCCTCTTCCG AGTGTCCACA GGTGACAACT GGAACGGCAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACA TGCTACAATA CAGTCATCTC CCCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG 
ATGAAGCACC TGGAGGAAAG CAACAAGGAG GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG 
CTGGAGCTGG AGATGAAGAC GCTCAGCCCA CAGCCCCACT CGCCACTGGG CAGCCCCTTC 
CTCTGGCCGG GGGTCGAGGG CCCCGACAGC CCCGATAGCC CCAAGCTCAG GGCCCCGCAC 
ACCGCGGCCC ACACGGGAGC AGCCTCGCGC TTCTCCCTGG AACACCCCAC GGACAGACAG 
CTGTTTGACA CCATATCCCT GCTGATCCAG GGCTCCCTGG AAGGGGAGCT GAAGCTGATG 
GACGAGCTGG CAGGCCCAGG GGGCCAGCCC TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC 
GGCTCCGACC CACAGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTCTGAC GTCAGAGATT 
GTGTCTGAAC CGTCCCACTC TCTAGCTCTG ACGGATGACT CTTTGCCTGA TGACACTCAC 
ACACTCTTAC CTAGTGCCCT GGACAGCAAT ATGGAGCCCC ACCCCGAGGA GATGCCCCTT 
GCACTGGGAC TGGACCTGCT GACTGTGAGG AAGTCTGGGG TCAGCCGGAC ACACTCCCTG 
CCCAATGACA GCTACATGTT CCGGGACGGA GGCACCGCTG AGGGGTCCCT AGGATGCAGA 
AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC TCTGTCTTAT CTGTCCACTC GCAGCCAGCA 
GACACCAGCT ACATCCTGCA GCTTCCCAAA GACACACCTC ACCTGCTCCA GCCCCACAGC 
GCTCCCACCT GGGGCACCAT TCCCAAACTG CCCCCACCAG GCCGCTCCCC CTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATAAGG ACTGATTCTC TGGACGTTCA GGGCCTGGGC 
AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT GGGCCCTCCC CACCTCTGGC CCGGGCCTCC 
TCCTTCTGGG GCCACTTGGG CGCCCAGATG CAGCAGCATC CCCACCGCCA GAGCAAGATC 
TCGAAGCACA TGCCCCCGCC AGCCCCCCGC CCAGGCTCAG AAACCACCTG GAGCAAAGAC 
CCACCCGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGACCG TGGGCAGCCA GGAGGAGCCC CCGTCCCCAC GGGACCTGAA GAAGTGTCAC 
AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG CCTGCGTCCT GGCTGGATGA ACAGAGGAGA 
CACTCCATCG CGGTCAGCTG CCTGGACGGC GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC 
TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA CCTGGAAGCC GGCCCAAGAA AAAACTCAGC 
CCACCTAGTA TCTCCATAGA CCCCCCAGAG AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC 
GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC AGCGACTCCA GGGACCCCTC GGCCTCCGGC 
CCCCCTGACA GCATGGCTGC CTCGCCCCCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT 
TTAGCCTCTG ACCCAGCAGA CCTGGACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624652.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624709.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X7, mRNA.

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CloneID	OCe11916
Clone ID Related Accession (Same CDS sequence)	XM_005624652.2 , XM_005624652.3
Accession Version	XM_005624652.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGAGGC CCAGTGTAAA 
CCCTACTACT CAGACTACTC CCGATTCCGG CTCCTCGTCC ACCACTTGTG TACCAGCCAC 
TATCTGGACC TCTTCATCAC GGGTGTCATT GGTCTGAATG TGGTCACCAT GGCCATGGAG 
CACTACCAGC AGCCCCAGAT CCTGGACGAG GCCTTGAAGA TCTGCAACTA TATCTTCACC 
GTCATCTTTG TCTTGGAGTC AGTTTTTAAA CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCC ATCGTGCTGC TGTCCATCAT GGGCATCACG 
CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG CCCATCAACC CCACCATCAT CCGCATCATG 
AGAGTGCTGC GCATTGCCCG AGTGCTGAAG CTGCTGAAGA TGGCTGTGGG CATGCGGGCG 
CTGCTGGACA CAGTCATGCA GGCCCTGCCT CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
TTGTTGTTTT TCATCTTTGC AGCTCTGGGT GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACGCACC CCTGCGAGGG CCTGGGCCGG CATGCCACCT TTCGGAACTT TGGCATGGCC 
TTCCTGACCC TCTTCCGAGT GTCCACAGGT GACAACTGGA ACGGCATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACATGC TACAATACAG TCATCTCCCC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTGATCGC TGTGCTGATG 
AAGCACCTGG AGGAAAGCAA CAAGGAGGCC AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG 
GAGCTGGAGA TGAAGACGCT CAGCCCACAG CCCCACTCGC CACTGGGCAG CCCCTTCCTC 
TGGCCGGGGG TCGAGGGCCC CGACAGCCCC GATAGCCCCA AGCTCAGGGC CCCGCACACC 
GCGGCCCACA CGGGAGCAGC CTCGCGCTTC TCCCTGGAAC ACCCCACGGA CAGACAGCTG 
TTTGACACCA TATCCCTGCT GATCCAGGGC TCCCTGGAAG GGGAGCTGAA GCTGATGGAC 
GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT GCCTTCCCTC CCGCCCGCAG CCTGGGCGGC 
TCCGACCCAC AGATCCCTCT AGCTGAGATG GAGGCTCTGT CTCTGACGTC AGAGATTGTG 
TCTGAACCGT CCCACTCTCT AGCTCTGACG GATGACTCTT TGCCTGATGA CACTCACACA 
CTCTTACCTA GTGCCCTGGA CAGCAATATG GAGCCCCACC CCGAGGAGAT GCCCCTTGCA 
CTGGGACTGG ACCTGCTGAC TGTGAGGAAG TCTGGGGTCA GCCGGACACA CTCCCTGCCC 
AATGACAGCT ACATGTTCCG GGACGGAGGC ACCGCTGAGG GGTCCCTAGG ATGCAGAAGC 
TGGGGGCTCC CCAAAGCTCA GTCAGGCTCT GTCTTATCTG TCCACTCGCA GCCAGCAGAC 
ACCAGCTACA TCCTGCAGCT TCCCAAAGAC ACACCTCACC TGCTCCAGCC CCACAGCGCT 
CCCACCTGGG GCACCATTCC CAAACTGCCC CCACCAGGCC GCTCCCCCTT GGCTCAGAGG 
CCACTCAGGC GCCAGGCAGC AATAAGGACT GATTCTCTGG ACGTTCAGGG CCTGGGCAGC 
CGGGAAGACC TGCTGTCCGA GGTGAGTGGG CCCTCCCCAC CTCTGGCCCG GGCCTCCTCC 
TTCTGGGGCC ACTTGGGCGC CCAGATGCAG CAGCATCCCC ACCGCCAGAG CAAGATCTCG 
AAGCACATGC CCCCGCCAGC CCCCCGCCCA GGCTCAGAAA CCACCTGGAG CAAAGACCCA 
CCCGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGACCGTGG GCAGCCAGGA GGAGCCCCCG TCCCCACGGG ACCTGAAGAA GTGTCACAGC 
GTGGAGGCCC AGAGCTGCCG GCGCAGGCCT GCGTCCTGGC TGGATGAACA GAGGAGACAC 
TCCATCGCGG TCAGCTGCCT GGACGGCGGC TCCCAACCCA GCCTGGGCCC CGGCCCCTCT 
AGCCTTGGCG GCCAGCCTCT TGGGGGACCT GGAAGCCGGC CCAAGAAAAA ACTCAGCCCA 
CCTAGTATCT CCATAGACCC CCCAGAGAGT CAGGGCCCTC GGCCCCCACC CAGCCCCGGC 
GTCTGCCTCC GGCGGAGGGC TCCGTCCAGC GACTCCAGGG ACCCCTCGGC CTCCGGCCCC 
CCTGACAGCA TGGCTGCCTC GCCCCCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
GCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624709.1
CDS	527..7636
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624652.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624652.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGAGGC CCAGTGTAAA 
CCCTACTACT CAGACTACTC CCGATTCCGG CTCCTCGTCC ACCACTTGTG TACCAGCCAC 
TATCTGGACC TCTTCATCAC GGGTGTCATT GGTCTGAATG TGGTCACCAT GGCCATGGAG 
CACTACCAGC AGCCCCAGAT CCTGGACGAG GCCTTGAAGA TCTGCAACTA TATCTTCACC 
GTCATCTTTG TCTTGGAGTC AGTTTTTAAA CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCC ATCGTGCTGC TGTCCATCAT GGGCATCACG 
CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG CCCATCAACC CCACCATCAT CCGCATCATG 
AGAGTGCTGC GCATTGCCCG AGTGCTGAAG CTGCTGAAGA TGGCTGTGGG CATGCGGGCG 
CTGCTGGACA CAGTCATGCA GGCCCTGCCT CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
TTGTTGTTTT TCATCTTTGC AGCTCTGGGT GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACGCACC CCTGCGAGGG CCTGGGCCGG CATGCCACCT TTCGGAACTT TGGCATGGCC 
TTCCTGACCC TCTTCCGAGT GTCCACAGGT GACAACTGGA ACGGCATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACATGC TACAATACAG TCATCTCCCC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTGATCGC TGTGCTGATG 
AAGCACCTGG AGGAAAGCAA CAAGGAGGCC AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG 
GAGCTGGAGA TGAAGACGCT CAGCCCACAG CCCCACTCGC CACTGGGCAG CCCCTTCCTC 
TGGCCGGGGG TCGAGGGCCC CGACAGCCCC GATAGCCCCA AGCTCAGGGC CCCGCACACC 
GCGGCCCACA CGGGAGCAGC CTCGCGCTTC TCCCTGGAAC ACCCCACGGA CAGACAGCTG 
TTTGACACCA TATCCCTGCT GATCCAGGGC TCCCTGGAAG GGGAGCTGAA GCTGATGGAC 
GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT GCCTTCCCTC CCGCCCGCAG CCTGGGCGGC 
TCCGACCCAC AGATCCCTCT AGCTGAGATG GAGGCTCTGT CTCTGACGTC AGAGATTGTG 
TCTGAACCGT CCCACTCTCT AGCTCTGACG GATGACTCTT TGCCTGATGA CACTCACACA 
CTCTTACCTA GTGCCCTGGA CAGCAATATG GAGCCCCACC CCGAGGAGAT GCCCCTTGCA 
CTGGGACTGG ACCTGCTGAC TGTGAGGAAG TCTGGGGTCA GCCGGACACA CTCCCTGCCC 
AATGACAGCT ACATGTTCCG GGACGGAGGC ACCGCTGAGG GGTCCCTAGG ATGCAGAAGC 
TGGGGGCTCC CCAAAGCTCA GTCAGGCTCT GTCTTATCTG TCCACTCGCA GCCAGCAGAC 
ACCAGCTACA TCCTGCAGCT TCCCAAAGAC ACACCTCACC TGCTCCAGCC CCACAGCGCT 
CCCACCTGGG GCACCATTCC CAAACTGCCC CCACCAGGCC GCTCCCCCTT GGCTCAGAGG 
CCACTCAGGC GCCAGGCAGC AATAAGGACT GATTCTCTGG ACGTTCAGGG CCTGGGCAGC 
CGGGAAGACC TGCTGTCCGA GGTGAGTGGG CCCTCCCCAC CTCTGGCCCG GGCCTCCTCC 
TTCTGGGGCC ACTTGGGCGC CCAGATGCAG CAGCATCCCC ACCGCCAGAG CAAGATCTCG 
AAGCACATGC CCCCGCCAGC CCCCCGCCCA GGCTCAGAAA CCACCTGGAG CAAAGACCCA 
CCCGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGACCGTGG GCAGCCAGGA GGAGCCCCCG TCCCCACGGG ACCTGAAGAA GTGTCACAGC 
GTGGAGGCCC AGAGCTGCCG GCGCAGGCCT GCGTCCTGGC TGGATGAACA GAGGAGACAC 
TCCATCGCGG TCAGCTGCCT GGACGGCGGC TCCCAACCCA GCCTGGGCCC CGGCCCCTCT 
AGCCTTGGCG GCCAGCCTCT TGGGGGACCT GGAAGCCGGC CCAAGAAAAA ACTCAGCCCA 
CCTAGTATCT CCATAGACCC CCCAGAGAGT CAGGGCCCTC GGCCCCCACC CAGCCCCGGC 
GTCTGCCTCC GGCGGAGGGC TCCGTCCAGC GACTCCAGGG ACCCCTCGGC CTCCGGCCCC 
CCTGACAGCA TGGCTGCCTC GCCCCCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
GCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624652.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624709.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X7, mRNA.

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CloneID	OCe11916
Clone ID Related Accession (Same CDS sequence)	XM_005624652.2 , XM_005624652.3
Accession Version	XM_005624652.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624652.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGAGGC CCAGTGTAAA 
CCCTACTACT CAGACTACTC CCGATTCCGG CTCCTCGTCC ACCACTTGTG TACCAGCCAC 
TATCTGGACC TCTTCATCAC GGGTGTCATT GGTCTGAATG TGGTCACCAT GGCCATGGAG 
CACTACCAGC AGCCCCAGAT CCTGGACGAG GCCTTGAAGA TCTGCAACTA TATCTTCACC 
GTCATCTTTG TCTTGGAGTC AGTTTTTAAA CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCC ATCGTGCTGC TGTCCATCAT GGGCATCACG 
CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG CCCATCAACC CCACCATCAT CCGCATCATG 
AGAGTGCTGC GCATTGCCCG AGTGCTGAAG CTGCTGAAGA TGGCTGTGGG CATGCGGGCG 
CTGCTGGACA CAGTCATGCA GGCCCTGCCT CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
TTGTTGTTTT TCATCTTTGC AGCTCTGGGT GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACGCACC CCTGCGAGGG CCTGGGCCGG CATGCCACCT TTCGGAACTT TGGCATGGCC 
TTCCTGACCC TCTTCCGAGT GTCCACAGGT GACAACTGGA ACGGCATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACATGC TACAATACAG TCATCTCCCC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTGATCGC TGTGCTGATG 
AAGCACCTGG AGGAAAGCAA CAAGGAGGCC AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG 
GAGCTGGAGA TGAAGACGCT CAGCCCACAG CCCCACTCGC CACTGGGCAG CCCCTTCCTC 
TGGCCGGGGG TCGAGGGCCC CGACAGCCCC GATAGCCCCA AGCTCAGGGC CCCGCACACC 
GCGGCCCACA CGGGAGCAGC CTCGCGCTTC TCCCTGGAAC ACCCCACGGA CAGACAGCTG 
TTTGACACCA TATCCCTGCT GATCCAGGGC TCCCTGGAAG GGGAGCTGAA GCTGATGGAC 
GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT GCCTTCCCTC CCGCCCGCAG CCTGGGCGGC 
TCCGACCCAC AGATCCCTCT AGCTGAGATG GAGGCTCTGT CTCTGACGTC AGAGATTGTG 
TCTGAACCGT CCCACTCTCT AGCTCTGACG GATGACTCTT TGCCTGATGA CACTCACACA 
CTCTTACCTA GTGCCCTGGA CAGCAATATG GAGCCCCACC CCGAGGAGAT GCCCCTTGCA 
CTGGGACTGG ACCTGCTGAC TGTGAGGAAG TCTGGGGTCA GCCGGACACA CTCCCTGCCC 
AATGACAGCT ACATGTTCCG GGACGGAGGC ACCGCTGAGG GGTCCCTAGG ATGCAGAAGC 
TGGGGGCTCC CCAAAGCTCA GTCAGGCTCT GTCTTATCTG TCCACTCGCA GCCAGCAGAC 
ACCAGCTACA TCCTGCAGCT TCCCAAAGAC ACACCTCACC TGCTCCAGCC CCACAGCGCT 
CCCACCTGGG GCACCATTCC CAAACTGCCC CCACCAGGCC GCTCCCCCTT GGCTCAGAGG 
CCACTCAGGC GCCAGGCAGC AATAAGGACT GATTCTCTGG ACGTTCAGGG CCTGGGCAGC 
CGGGAAGACC TGCTGTCCGA GGTGAGTGGG CCCTCCCCAC CTCTGGCCCG GGCCTCCTCC 
TTCTGGGGCC ACTTGGGCGC CCAGATGCAG CAGCATCCCC ACCGCCAGAG CAAGATCTCG 
AAGCACATGC CCCCGCCAGC CCCCCGCCCA GGCTCAGAAA CCACCTGGAG CAAAGACCCA 
CCCGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGACCGTGG GCAGCCAGGA GGAGCCCCCG TCCCCACGGG ACCTGAAGAA GTGTCACAGC 
GTGGAGGCCC AGAGCTGCCG GCGCAGGCCT GCGTCCTGGC TGGATGAACA GAGGAGACAC 
TCCATCGCGG TCAGCTGCCT GGACGGCGGC TCCCAACCCA GCCTGGGCCC CGGCCCCTCT 
AGCCTTGGCG GCCAGCCTCT TGGGGGACCT GGAAGCCGGC CCAAGAAAAA ACTCAGCCCA 
CCTAGTATCT CCATAGACCC CCCAGAGAGT CAGGGCCCTC GGCCCCCACC CAGCCCCGGC 
GTCTGCCTCC GGCGGAGGGC TCCGTCCAGC GACTCCAGGG ACCCCTCGGC CTCCGGCCCC 
CCTGACAGCA TGGCTGCCTC GCCCCCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
GCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624709.1
CDS	504..7613
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624652.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624652.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGAGGC CCAGTGTAAA 
CCCTACTACT CAGACTACTC CCGATTCCGG CTCCTCGTCC ACCACTTGTG TACCAGCCAC 
TATCTGGACC TCTTCATCAC GGGTGTCATT GGTCTGAATG TGGTCACCAT GGCCATGGAG 
CACTACCAGC AGCCCCAGAT CCTGGACGAG GCCTTGAAGA TCTGCAACTA TATCTTCACC 
GTCATCTTTG TCTTGGAGTC AGTTTTTAAA CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCC ATCGTGCTGC TGTCCATCAT GGGCATCACG 
CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG CCCATCAACC CCACCATCAT CCGCATCATG 
AGAGTGCTGC GCATTGCCCG AGTGCTGAAG CTGCTGAAGA TGGCTGTGGG CATGCGGGCG 
CTGCTGGACA CAGTCATGCA GGCCCTGCCT CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
TTGTTGTTTT TCATCTTTGC AGCTCTGGGT GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACGCACC CCTGCGAGGG CCTGGGCCGG CATGCCACCT TTCGGAACTT TGGCATGGCC 
TTCCTGACCC TCTTCCGAGT GTCCACAGGT GACAACTGGA ACGGCATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACATGC TACAATACAG TCATCTCCCC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTGATCGC TGTGCTGATG 
AAGCACCTGG AGGAAAGCAA CAAGGAGGCC AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG 
GAGCTGGAGA TGAAGACGCT CAGCCCACAG CCCCACTCGC CACTGGGCAG CCCCTTCCTC 
TGGCCGGGGG TCGAGGGCCC CGACAGCCCC GATAGCCCCA AGCTCAGGGC CCCGCACACC 
GCGGCCCACA CGGGAGCAGC CTCGCGCTTC TCCCTGGAAC ACCCCACGGA CAGACAGCTG 
TTTGACACCA TATCCCTGCT GATCCAGGGC TCCCTGGAAG GGGAGCTGAA GCTGATGGAC 
GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT GCCTTCCCTC CCGCCCGCAG CCTGGGCGGC 
TCCGACCCAC AGATCCCTCT AGCTGAGATG GAGGCTCTGT CTCTGACGTC AGAGATTGTG 
TCTGAACCGT CCCACTCTCT AGCTCTGACG GATGACTCTT TGCCTGATGA CACTCACACA 
CTCTTACCTA GTGCCCTGGA CAGCAATATG GAGCCCCACC CCGAGGAGAT GCCCCTTGCA 
CTGGGACTGG ACCTGCTGAC TGTGAGGAAG TCTGGGGTCA GCCGGACACA CTCCCTGCCC 
AATGACAGCT ACATGTTCCG GGACGGAGGC ACCGCTGAGG GGTCCCTAGG ATGCAGAAGC 
TGGGGGCTCC CCAAAGCTCA GTCAGGCTCT GTCTTATCTG TCCACTCGCA GCCAGCAGAC 
ACCAGCTACA TCCTGCAGCT TCCCAAAGAC ACACCTCACC TGCTCCAGCC CCACAGCGCT 
CCCACCTGGG GCACCATTCC CAAACTGCCC CCACCAGGCC GCTCCCCCTT GGCTCAGAGG 
CCACTCAGGC GCCAGGCAGC AATAAGGACT GATTCTCTGG ACGTTCAGGG CCTGGGCAGC 
CGGGAAGACC TGCTGTCCGA GGTGAGTGGG CCCTCCCCAC CTCTGGCCCG GGCCTCCTCC 
TTCTGGGGCC ACTTGGGCGC CCAGATGCAG CAGCATCCCC ACCGCCAGAG CAAGATCTCG 
AAGCACATGC CCCCGCCAGC CCCCCGCCCA GGCTCAGAAA CCACCTGGAG CAAAGACCCA 
CCCGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGACCGTGG GCAGCCAGGA GGAGCCCCCG TCCCCACGGG ACCTGAAGAA GTGTCACAGC 
GTGGAGGCCC AGAGCTGCCG GCGCAGGCCT GCGTCCTGGC TGGATGAACA GAGGAGACAC 
TCCATCGCGG TCAGCTGCCT GGACGGCGGC TCCCAACCCA GCCTGGGCCC CGGCCCCTCT 
AGCCTTGGCG GCCAGCCTCT TGGGGGACCT GGAAGCCGGC CCAAGAAAAA ACTCAGCCCA 
CCTAGTATCT CCATAGACCC CCCAGAGAGT CAGGGCCCTC GGCCCCCACC CAGCCCCGGC 
GTCTGCCTCC GGCGGAGGGC TCCGTCCAGC GACTCCAGGG ACCCCTCGGC CTCCGGCCCC 
CCTGACAGCA TGGCTGCCTC GCCCCCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
GCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624653.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624710.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X10, mRNA.

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CloneID	OCe11917
Clone ID Related Accession (Same CDS sequence)	XM_005624653.2 , XM_005624653.3
Accession Version	XM_005624653.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG GACAGACAGC TGTTTGACAC CATATCCCTG 
CTGATCCAGG GCTCCCTGGA AGGGGAGCTG AAGCTGATGG ACGAGCTGGC AGGCCCAGGG 
GGCCAGCCCT CTGCCTTCCC TCCCGCCCGC AGCCTGGGCG GCTCCGACCC ACAGATCCCT 
CTAGCTGAGA TGGAGGCTCT GTCTCTGACG TCAGAGATTG TGTCTGAACC GTCCCACTCT 
CTAGCTCTGA CGGATGACTC TTTGCCTGAT GACACTCACA CACTCTTACC TAGTGCCCTG 
GACAGCAATA TGGAGCCCCA CCCCGAGGAG ATGCCCCTTG CACTGGGACT GGACCTGCTG 
ACTGTGAGGA AGTCTGGGGT CAGCCGGACA CACTCCCTGC CCAATGACAG CTACATGTTC 
CGGGACGGAG GCACCGCTGA GGGGTCCCTA GGATGCAGAA GCTGGGGGCT CCCCAAAGCT 
CAGTCAGGCT CTGTCTTATC TGTCCACTCG CAGCCAGCAG ACACCAGCTA CATCCTGCAG 
CTTCCCAAAG ACACACCTCA CCTGCTCCAG CCCCACAGCG CTCCCACCTG GGGCACCATT 
CCCAAACTGC CCCCACCAGG CCGCTCCCCC TTGGCTCAGA GGCCACTCAG GCGCCAGGCA 
GCAATAAGGA CTGATTCTCT GGACGTTCAG GGCCTGGGCA GCCGGGAAGA CCTGCTGTCC 
GAGGTGAGTG GGCCCTCCCC ACCTCTGGCC CGGGCCTCCT CCTTCTGGGG CCACTTGGGC 
GCCCAGATGC AGCAGCATCC CCACCGCCAG AGCAAGATCT CGAAGCACAT GCCCCCGCCA 
GCCCCCCGCC CAGGCTCAGA AACCACCTGG AGCAAAGACC CACCCGAGAC CAGAAGCAGC 
TTAGAGCTGG ACACGGAGCT GAGCTGGATT TCAGGAGACC TCCTGACCGT GGGCAGCCAG 
GAGGAGCCCC CGTCCCCACG GGACCTGAAG AAGTGTCACA GCGTGGAGGC CCAGAGCTGC 
CGGCGCAGGC CTGCGTCCTG GCTGGATGAA CAGAGGAGAC ACTCCATCGC GGTCAGCTGC 
CTGGACGGCG GCTCCCAACC CAGCCTGGGC CCCGGCCCCT CTAGCCTTGG CGGCCAGCCT 
CTTGGGGGAC CTGGAAGCCG GCCCAAGAAA AAACTCAGCC CACCTAGTAT CTCCATAGAC 
CCCCCAGAGA GTCAGGGCCC TCGGCCCCCA CCCAGCCCCG GCGTCTGCCT CCGGCGGAGG 
GCTCCGTCCA GCGACTCCAG GGACCCCTCG GCCTCCGGCC CCCCTGACAG CATGGCTGCC 
TCGCCCCCCC CAAAGAAAGA CGTGCTGAGT CTCTCTGGTT TAGCCTCTGA CCCAGCAGAC 
CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624710.1
CDS	527..7618
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624653.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624653.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG GACAGACAGC TGTTTGACAC CATATCCCTG 
CTGATCCAGG GCTCCCTGGA AGGGGAGCTG AAGCTGATGG ACGAGCTGGC AGGCCCAGGG 
GGCCAGCCCT CTGCCTTCCC TCCCGCCCGC AGCCTGGGCG GCTCCGACCC ACAGATCCCT 
CTAGCTGAGA TGGAGGCTCT GTCTCTGACG TCAGAGATTG TGTCTGAACC GTCCCACTCT 
CTAGCTCTGA CGGATGACTC TTTGCCTGAT GACACTCACA CACTCTTACC TAGTGCCCTG 
GACAGCAATA TGGAGCCCCA CCCCGAGGAG ATGCCCCTTG CACTGGGACT GGACCTGCTG 
ACTGTGAGGA AGTCTGGGGT CAGCCGGACA CACTCCCTGC CCAATGACAG CTACATGTTC 
CGGGACGGAG GCACCGCTGA GGGGTCCCTA GGATGCAGAA GCTGGGGGCT CCCCAAAGCT 
CAGTCAGGCT CTGTCTTATC TGTCCACTCG CAGCCAGCAG ACACCAGCTA CATCCTGCAG 
CTTCCCAAAG ACACACCTCA CCTGCTCCAG CCCCACAGCG CTCCCACCTG GGGCACCATT 
CCCAAACTGC CCCCACCAGG CCGCTCCCCC TTGGCTCAGA GGCCACTCAG GCGCCAGGCA 
GCAATAAGGA CTGATTCTCT GGACGTTCAG GGCCTGGGCA GCCGGGAAGA CCTGCTGTCC 
GAGGTGAGTG GGCCCTCCCC ACCTCTGGCC CGGGCCTCCT CCTTCTGGGG CCACTTGGGC 
GCCCAGATGC AGCAGCATCC CCACCGCCAG AGCAAGATCT CGAAGCACAT GCCCCCGCCA 
GCCCCCCGCC CAGGCTCAGA AACCACCTGG AGCAAAGACC CACCCGAGAC CAGAAGCAGC 
TTAGAGCTGG ACACGGAGCT GAGCTGGATT TCAGGAGACC TCCTGACCGT GGGCAGCCAG 
GAGGAGCCCC CGTCCCCACG GGACCTGAAG AAGTGTCACA GCGTGGAGGC CCAGAGCTGC 
CGGCGCAGGC CTGCGTCCTG GCTGGATGAA CAGAGGAGAC ACTCCATCGC GGTCAGCTGC 
CTGGACGGCG GCTCCCAACC CAGCCTGGGC CCCGGCCCCT CTAGCCTTGG CGGCCAGCCT 
CTTGGGGGAC CTGGAAGCCG GCCCAAGAAA AAACTCAGCC CACCTAGTAT CTCCATAGAC 
CCCCCAGAGA GTCAGGGCCC TCGGCCCCCA CCCAGCCCCG GCGTCTGCCT CCGGCGGAGG 
GCTCCGTCCA GCGACTCCAG GGACCCCTCG GCCTCCGGCC CCCCTGACAG CATGGCTGCC 
TCGCCCCCCC CAAAGAAAGA CGTGCTGAGT CTCTCTGGTT TAGCCTCTGA CCCAGCAGAC 
CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624653.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624710.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X8, mRNA.

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CloneID	OCe11917
Clone ID Related Accession (Same CDS sequence)	XM_005624653.2 , XM_005624653.3
Accession Version	XM_005624653.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624653.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG GACAGACAGC TGTTTGACAC CATATCCCTG 
CTGATCCAGG GCTCCCTGGA AGGGGAGCTG AAGCTGATGG ACGAGCTGGC AGGCCCAGGG 
GGCCAGCCCT CTGCCTTCCC TCCCGCCCGC AGCCTGGGCG GCTCCGACCC ACAGATCCCT 
CTAGCTGAGA TGGAGGCTCT GTCTCTGACG TCAGAGATTG TGTCTGAACC GTCCCACTCT 
CTAGCTCTGA CGGATGACTC TTTGCCTGAT GACACTCACA CACTCTTACC TAGTGCCCTG 
GACAGCAATA TGGAGCCCCA CCCCGAGGAG ATGCCCCTTG CACTGGGACT GGACCTGCTG 
ACTGTGAGGA AGTCTGGGGT CAGCCGGACA CACTCCCTGC CCAATGACAG CTACATGTTC 
CGGGACGGAG GCACCGCTGA GGGGTCCCTA GGATGCAGAA GCTGGGGGCT CCCCAAAGCT 
CAGTCAGGCT CTGTCTTATC TGTCCACTCG CAGCCAGCAG ACACCAGCTA CATCCTGCAG 
CTTCCCAAAG ACACACCTCA CCTGCTCCAG CCCCACAGCG CTCCCACCTG GGGCACCATT 
CCCAAACTGC CCCCACCAGG CCGCTCCCCC TTGGCTCAGA GGCCACTCAG GCGCCAGGCA 
GCAATAAGGA CTGATTCTCT GGACGTTCAG GGCCTGGGCA GCCGGGAAGA CCTGCTGTCC 
GAGGTGAGTG GGCCCTCCCC ACCTCTGGCC CGGGCCTCCT CCTTCTGGGG CCACTTGGGC 
GCCCAGATGC AGCAGCATCC CCACCGCCAG AGCAAGATCT CGAAGCACAT GCCCCCGCCA 
GCCCCCCGCC CAGGCTCAGA AACCACCTGG AGCAAAGACC CACCCGAGAC CAGAAGCAGC 
TTAGAGCTGG ACACGGAGCT GAGCTGGATT TCAGGAGACC TCCTGACCGT GGGCAGCCAG 
GAGGAGCCCC CGTCCCCACG GGACCTGAAG AAGTGTCACA GCGTGGAGGC CCAGAGCTGC 
CGGCGCAGGC CTGCGTCCTG GCTGGATGAA CAGAGGAGAC ACTCCATCGC GGTCAGCTGC 
CTGGACGGCG GCTCCCAACC CAGCCTGGGC CCCGGCCCCT CTAGCCTTGG CGGCCAGCCT 
CTTGGGGGAC CTGGAAGCCG GCCCAAGAAA AAACTCAGCC CACCTAGTAT CTCCATAGAC 
CCCCCAGAGA GTCAGGGCCC TCGGCCCCCA CCCAGCCCCG GCGTCTGCCT CCGGCGGAGG 
GCTCCGTCCA GCGACTCCAG GGACCCCTCG GCCTCCGGCC CCCCTGACAG CATGGCTGCC 
TCGCCCCCCC CAAAGAAAGA CGTGCTGAGT CTCTCTGGTT TAGCCTCTGA CCCAGCAGAC 
CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624710.1
CDS	505..7596
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624653.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624653.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG GACAGACAGC TGTTTGACAC CATATCCCTG 
CTGATCCAGG GCTCCCTGGA AGGGGAGCTG AAGCTGATGG ACGAGCTGGC AGGCCCAGGG 
GGCCAGCCCT CTGCCTTCCC TCCCGCCCGC AGCCTGGGCG GCTCCGACCC ACAGATCCCT 
CTAGCTGAGA TGGAGGCTCT GTCTCTGACG TCAGAGATTG TGTCTGAACC GTCCCACTCT 
CTAGCTCTGA CGGATGACTC TTTGCCTGAT GACACTCACA CACTCTTACC TAGTGCCCTG 
GACAGCAATA TGGAGCCCCA CCCCGAGGAG ATGCCCCTTG CACTGGGACT GGACCTGCTG 
ACTGTGAGGA AGTCTGGGGT CAGCCGGACA CACTCCCTGC CCAATGACAG CTACATGTTC 
CGGGACGGAG GCACCGCTGA GGGGTCCCTA GGATGCAGAA GCTGGGGGCT CCCCAAAGCT 
CAGTCAGGCT CTGTCTTATC TGTCCACTCG CAGCCAGCAG ACACCAGCTA CATCCTGCAG 
CTTCCCAAAG ACACACCTCA CCTGCTCCAG CCCCACAGCG CTCCCACCTG GGGCACCATT 
CCCAAACTGC CCCCACCAGG CCGCTCCCCC TTGGCTCAGA GGCCACTCAG GCGCCAGGCA 
GCAATAAGGA CTGATTCTCT GGACGTTCAG GGCCTGGGCA GCCGGGAAGA CCTGCTGTCC 
GAGGTGAGTG GGCCCTCCCC ACCTCTGGCC CGGGCCTCCT CCTTCTGGGG CCACTTGGGC 
GCCCAGATGC AGCAGCATCC CCACCGCCAG AGCAAGATCT CGAAGCACAT GCCCCCGCCA 
GCCCCCCGCC CAGGCTCAGA AACCACCTGG AGCAAAGACC CACCCGAGAC CAGAAGCAGC 
TTAGAGCTGG ACACGGAGCT GAGCTGGATT TCAGGAGACC TCCTGACCGT GGGCAGCCAG 
GAGGAGCCCC CGTCCCCACG GGACCTGAAG AAGTGTCACA GCGTGGAGGC CCAGAGCTGC 
CGGCGCAGGC CTGCGTCCTG GCTGGATGAA CAGAGGAGAC ACTCCATCGC GGTCAGCTGC 
CTGGACGGCG GCTCCCAACC CAGCCTGGGC CCCGGCCCCT CTAGCCTTGG CGGCCAGCCT 
CTTGGGGGAC CTGGAAGCCG GCCCAAGAAA AAACTCAGCC CACCTAGTAT CTCCATAGAC 
CCCCCAGAGA GTCAGGGCCC TCGGCCCCCA CCCAGCCCCG GCGTCTGCCT CCGGCGGAGG 
GCTCCGTCCA GCGACTCCAG GGACCCCTCG GCCTCCGGCC CCCCTGACAG CATGGCTGCC 
TCGCCCCCCC CAAAGAAAGA CGTGCTGAGT CTCTCTGGTT TAGCCTCTGA CCCAGCAGAC 
CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860714.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865807.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X10, mRNA.

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CloneID	OCe11918
Clone ID Related Accession (Same CDS sequence)	XM_860714.5 , XM_860714.4
Accession Version	XM_860714.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_860714.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGAGG CCCAGTGTAA ACCCTACTAC 
TCAGACTACT CCCGATTCCG GCTCCTCGTC CACCACTTGT GTACCAGCCA CTATCTGGAC 
CTCTTCATCA CGGGTGTCAT TGGTCTGAAT GTGGTCACCA TGGCCATGGA GCACTACCAG 
CAGCCCCAGA TCCTGGACGA GGCCTTGAAG ATCTGCAACT ATATCTTCAC CGTCATCTTT 
GTCTTGGAGT CAGTTTTTAA ACTCGTGGCC TTTGGCTTCC GTCGGTTCTT CCAGGACAGG 
TGGAACCAGC TGGACCTGGC CATCGTGCTG CTGTCCATCA TGGGCATCAC GCTGGAGGAG 
ATTGAGGTCA ATGCCTCGCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGAGTGCTG 
CGCATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCTGTGG GCATGCGGGC GCTGCTGGAC 
ACAGTCATGC AGGCCCTGCC TCAGGTGGGG AACCTGGGAC TTCTCTTCAT GTTGTTGTTT 
TTCATCTTTG CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC 
CCCTGCGAGG GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGACC 
CTCTTCCGAG TGTCCACAGG TGACAACTGG AACGGCATTA TGAAGGACAC CCTCCGGGAC 
TGTGACCAGG AGTCCACATG CTACAATACA GTCATCTCCC CCATCTACTT CGTGTCCTTC 
GTGCTGACGG CCCAGTTTGT GCTGGTCAAC GTGGTGATCG CTGTGCTGAT GAAGCACCTG 
GAGGAAAGCA ACAAGGAGGC CAAAGAGGAG GCCGAGCTGG AGGCTGAGCT GGAGCTGGAG 
ATGAAGACGC TCAGCCCACA GCCCCACTCG CCACTGGGCA GCCCCTTCCT CTGGCCGGGG 
GTCGAGGGCC CCGACAGCCC CGATAGCCCC AAGCTCAGGG CCCCGCACAC CGCGGCCCAC 
ACGGGAGCAG CCTCGCGCTT CTCCCTGGAA CACCCCACGG ACAGACAGCT GTTTGACACC 
ATATCCCTGC TGATCCAGGG CTCCCTGGAA GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT CCCGCCCGCA GCCTGGGCGG CTCCGACCCA 
CAGATCCCTC TAGCTGAGAT GGAGGCTCTG TCTCTGACGT CAGAGATTGT GTCTGAACCG 
TCCCACTCTC TAGCTCTGAC GGATGACTCT TTGCCTGATG ACACTCACAC ACTCTTACCT 
AGTGCCCTGG ACAGCAATAT GGAGCCCCAC CCCGAGGAGA TGCCCCTTGC ACTGGGACTG 
GACCTGCTGA CTGTGAGGAA GTCTGGGGTC AGCCGGACAC ACTCCCTGCC CAATGACAGC 
TACATGTTCC GGGACGGAGG CACCGCTGAG GGGTCCCTAG GATGCAGAAG CTGGGGGCTC 
CCCAAAGCTC AGTCAGGCTC TGTCTTATCT GTCCACTCGC AGCCAGCAGA CACCAGCTAC 
ATCCTGCAGC TTCCCAAAGA CACACCTCAC CTGCTCCAGC CCCACAGCGC TCCCACCTGG 
GGCACCATTC CCAAACTGCC CCCACCAGGC CGCTCCCCCT TGGCTCAGAG GCCACTCAGG 
CGCCAGGCAG CAATAAGGAC TGATTCTCTG GACGTTCAGG GCCTGGGCAG CCGGGAAGAC 
CTGCTGTCCG AGGTGAGTGG GCCCTCCCCA CCTCTGGCCC GGGCCTCCTC CTTCTGGGGC 
CACTTGGGCG CCCAGATGCA GCAGCATCCC CACCGCCAGA GCAAGATCTC GAAGCACATG 
CCCCCGCCAG CCCCCCGCCC AGGCTCAGAA ACCACCTGGA GCAAAGACCC ACCCGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGACCGTG 
GGCAGCCAGG AGGAGCCCCC GTCCCCACGG GACCTGAAGA AGTGTCACAG CGTGGAGGCC 
CAGAGCTGCC GGCGCAGGCC TGCGTCCTGG CTGGATGAAC AGAGGAGACA CTCCATCGCG 
GTCAGCTGCC TGGACGGCGG CTCCCAACCC AGCCTGGGCC CCGGCCCCTC TAGCCTTGGC 
GGCCAGCCTC TTGGGGGACC TGGAAGCCGG CCCAAGAAAA AACTCAGCCC ACCTAGTATC 
TCCATAGACC CCCCAGAGAG TCAGGGCCCT CGGCCCCCAC CCAGCCCCGG CGTCTGCCTC 
CGGCGGAGGG CTCCGTCCAG CGACTCCAGG GACCCCTCGG CCTCCGGCCC CCCTGACAGC 
ATGGCTGCCT CGCCCCCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT AGCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865807.1
CDS	504..7544
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860714.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860714.5

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGAGG CCCAGTGTAA ACCCTACTAC 
TCAGACTACT CCCGATTCCG GCTCCTCGTC CACCACTTGT GTACCAGCCA CTATCTGGAC 
CTCTTCATCA CGGGTGTCAT TGGTCTGAAT GTGGTCACCA TGGCCATGGA GCACTACCAG 
CAGCCCCAGA TCCTGGACGA GGCCTTGAAG ATCTGCAACT ATATCTTCAC CGTCATCTTT 
GTCTTGGAGT CAGTTTTTAA ACTCGTGGCC TTTGGCTTCC GTCGGTTCTT CCAGGACAGG 
TGGAACCAGC TGGACCTGGC CATCGTGCTG CTGTCCATCA TGGGCATCAC GCTGGAGGAG 
ATTGAGGTCA ATGCCTCGCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGAGTGCTG 
CGCATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCTGTGG GCATGCGGGC GCTGCTGGAC 
ACAGTCATGC AGGCCCTGCC TCAGGTGGGG AACCTGGGAC TTCTCTTCAT GTTGTTGTTT 
TTCATCTTTG CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC 
CCCTGCGAGG GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGACC 
CTCTTCCGAG TGTCCACAGG TGACAACTGG AACGGCATTA TGAAGGACAC CCTCCGGGAC 
TGTGACCAGG AGTCCACATG CTACAATACA GTCATCTCCC CCATCTACTT CGTGTCCTTC 
GTGCTGACGG CCCAGTTTGT GCTGGTCAAC GTGGTGATCG CTGTGCTGAT GAAGCACCTG 
GAGGAAAGCA ACAAGGAGGC CAAAGAGGAG GCCGAGCTGG AGGCTGAGCT GGAGCTGGAG 
ATGAAGACGC TCAGCCCACA GCCCCACTCG CCACTGGGCA GCCCCTTCCT CTGGCCGGGG 
GTCGAGGGCC CCGACAGCCC CGATAGCCCC AAGCTCAGGG CCCCGCACAC CGCGGCCCAC 
ACGGGAGCAG CCTCGCGCTT CTCCCTGGAA CACCCCACGG ACAGACAGCT GTTTGACACC 
ATATCCCTGC TGATCCAGGG CTCCCTGGAA GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT CCCGCCCGCA GCCTGGGCGG CTCCGACCCA 
CAGATCCCTC TAGCTGAGAT GGAGGCTCTG TCTCTGACGT CAGAGATTGT GTCTGAACCG 
TCCCACTCTC TAGCTCTGAC GGATGACTCT TTGCCTGATG ACACTCACAC ACTCTTACCT 
AGTGCCCTGG ACAGCAATAT GGAGCCCCAC CCCGAGGAGA TGCCCCTTGC ACTGGGACTG 
GACCTGCTGA CTGTGAGGAA GTCTGGGGTC AGCCGGACAC ACTCCCTGCC CAATGACAGC 
TACATGTTCC GGGACGGAGG CACCGCTGAG GGGTCCCTAG GATGCAGAAG CTGGGGGCTC 
CCCAAAGCTC AGTCAGGCTC TGTCTTATCT GTCCACTCGC AGCCAGCAGA CACCAGCTAC 
ATCCTGCAGC TTCCCAAAGA CACACCTCAC CTGCTCCAGC CCCACAGCGC TCCCACCTGG 
GGCACCATTC CCAAACTGCC CCCACCAGGC CGCTCCCCCT TGGCTCAGAG GCCACTCAGG 
CGCCAGGCAG CAATAAGGAC TGATTCTCTG GACGTTCAGG GCCTGGGCAG CCGGGAAGAC 
CTGCTGTCCG AGGTGAGTGG GCCCTCCCCA CCTCTGGCCC GGGCCTCCTC CTTCTGGGGC 
CACTTGGGCG CCCAGATGCA GCAGCATCCC CACCGCCAGA GCAAGATCTC GAAGCACATG 
CCCCCGCCAG CCCCCCGCCC AGGCTCAGAA ACCACCTGGA GCAAAGACCC ACCCGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGACCGTG 
GGCAGCCAGG AGGAGCCCCC GTCCCCACGG GACCTGAAGA AGTGTCACAG CGTGGAGGCC 
CAGAGCTGCC GGCGCAGGCC TGCGTCCTGG CTGGATGAAC AGAGGAGACA CTCCATCGCG 
GTCAGCTGCC TGGACGGCGG CTCCCAACCC AGCCTGGGCC CCGGCCCCTC TAGCCTTGGC 
GGCCAGCCTC TTGGGGGACC TGGAAGCCGG CCCAAGAAAA AACTCAGCCC ACCTAGTATC 
TCCATAGACC CCCCAGAGAG TCAGGGCCCT CGGCCCCCAC CCAGCCCCGG CGTCTGCCTC 
CGGCGGAGGG CTCCGTCCAG CGACTCCAGG GACCCCTCGG CCTCCGGCCC CCCTGACAGC 
ATGGCTGCCT CGCCCCCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT AGCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860714.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865807.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X12, mRNA.

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CloneID	OCe11918
Clone ID Related Accession (Same CDS sequence)	XM_860714.5 , XM_860714.4
Accession Version	XM_860714.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_860714.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGAGG CCCAGTGTAA ACCCTACTAC 
TCAGACTACT CCCGATTCCG GCTCCTCGTC CACCACTTGT GTACCAGCCA CTATCTGGAC 
CTCTTCATCA CGGGTGTCAT TGGTCTGAAT GTGGTCACCA TGGCCATGGA GCACTACCAG 
CAGCCCCAGA TCCTGGACGA GGCCTTGAAG ATCTGCAACT ATATCTTCAC CGTCATCTTT 
GTCTTGGAGT CAGTTTTTAA ACTCGTGGCC TTTGGCTTCC GTCGGTTCTT CCAGGACAGG 
TGGAACCAGC TGGACCTGGC CATCGTGCTG CTGTCCATCA TGGGCATCAC GCTGGAGGAG 
ATTGAGGTCA ATGCCTCGCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGAGTGCTG 
CGCATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCTGTGG GCATGCGGGC GCTGCTGGAC 
ACAGTCATGC AGGCCCTGCC TCAGGTGGGG AACCTGGGAC TTCTCTTCAT GTTGTTGTTT 
TTCATCTTTG CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC 
CCCTGCGAGG GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGACC 
CTCTTCCGAG TGTCCACAGG TGACAACTGG AACGGCATTA TGAAGGACAC CCTCCGGGAC 
TGTGACCAGG AGTCCACATG CTACAATACA GTCATCTCCC CCATCTACTT CGTGTCCTTC 
GTGCTGACGG CCCAGTTTGT GCTGGTCAAC GTGGTGATCG CTGTGCTGAT GAAGCACCTG 
GAGGAAAGCA ACAAGGAGGC CAAAGAGGAG GCCGAGCTGG AGGCTGAGCT GGAGCTGGAG 
ATGAAGACGC TCAGCCCACA GCCCCACTCG CCACTGGGCA GCCCCTTCCT CTGGCCGGGG 
GTCGAGGGCC CCGACAGCCC CGATAGCCCC AAGCTCAGGG CCCCGCACAC CGCGGCCCAC 
ACGGGAGCAG CCTCGCGCTT CTCCCTGGAA CACCCCACGG ACAGACAGCT GTTTGACACC 
ATATCCCTGC TGATCCAGGG CTCCCTGGAA GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT CCCGCCCGCA GCCTGGGCGG CTCCGACCCA 
CAGATCCCTC TAGCTGAGAT GGAGGCTCTG TCTCTGACGT CAGAGATTGT GTCTGAACCG 
TCCCACTCTC TAGCTCTGAC GGATGACTCT TTGCCTGATG ACACTCACAC ACTCTTACCT 
AGTGCCCTGG ACAGCAATAT GGAGCCCCAC CCCGAGGAGA TGCCCCTTGC ACTGGGACTG 
GACCTGCTGA CTGTGAGGAA GTCTGGGGTC AGCCGGACAC ACTCCCTGCC CAATGACAGC 
TACATGTTCC GGGACGGAGG CACCGCTGAG GGGTCCCTAG GATGCAGAAG CTGGGGGCTC 
CCCAAAGCTC AGTCAGGCTC TGTCTTATCT GTCCACTCGC AGCCAGCAGA CACCAGCTAC 
ATCCTGCAGC TTCCCAAAGA CACACCTCAC CTGCTCCAGC CCCACAGCGC TCCCACCTGG 
GGCACCATTC CCAAACTGCC CCCACCAGGC CGCTCCCCCT TGGCTCAGAG GCCACTCAGG 
CGCCAGGCAG CAATAAGGAC TGATTCTCTG GACGTTCAGG GCCTGGGCAG CCGGGAAGAC 
CTGCTGTCCG AGGTGAGTGG GCCCTCCCCA CCTCTGGCCC GGGCCTCCTC CTTCTGGGGC 
CACTTGGGCG CCCAGATGCA GCAGCATCCC CACCGCCAGA GCAAGATCTC GAAGCACATG 
CCCCCGCCAG CCCCCCGCCC AGGCTCAGAA ACCACCTGGA GCAAAGACCC ACCCGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGACCGTG 
GGCAGCCAGG AGGAGCCCCC GTCCCCACGG GACCTGAAGA AGTGTCACAG CGTGGAGGCC 
CAGAGCTGCC GGCGCAGGCC TGCGTCCTGG CTGGATGAAC AGAGGAGACA CTCCATCGCG 
GTCAGCTGCC TGGACGGCGG CTCCCAACCC AGCCTGGGCC CCGGCCCCTC TAGCCTTGGC 
GGCCAGCCTC TTGGGGGACC TGGAAGCCGG CCCAAGAAAA AACTCAGCCC ACCTAGTATC 
TCCATAGACC CCCCAGAGAG TCAGGGCCCT CGGCCCCCAC CCAGCCCCGG CGTCTGCCTC 
CGGCGGAGGG CTCCGTCCAG CGACTCCAGG GACCCCTCGG CCTCCGGCCC CCCTGACAGC 
ATGGCTGCCT CGCCCCCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT AGCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865807.1
CDS	527..7567
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860714.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860714.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGAGG CCCAGTGTAA ACCCTACTAC 
TCAGACTACT CCCGATTCCG GCTCCTCGTC CACCACTTGT GTACCAGCCA CTATCTGGAC 
CTCTTCATCA CGGGTGTCAT TGGTCTGAAT GTGGTCACCA TGGCCATGGA GCACTACCAG 
CAGCCCCAGA TCCTGGACGA GGCCTTGAAG ATCTGCAACT ATATCTTCAC CGTCATCTTT 
GTCTTGGAGT CAGTTTTTAA ACTCGTGGCC TTTGGCTTCC GTCGGTTCTT CCAGGACAGG 
TGGAACCAGC TGGACCTGGC CATCGTGCTG CTGTCCATCA TGGGCATCAC GCTGGAGGAG 
ATTGAGGTCA ATGCCTCGCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGAGTGCTG 
CGCATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCTGTGG GCATGCGGGC GCTGCTGGAC 
ACAGTCATGC AGGCCCTGCC TCAGGTGGGG AACCTGGGAC TTCTCTTCAT GTTGTTGTTT 
TTCATCTTTG CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC 
CCCTGCGAGG GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGACC 
CTCTTCCGAG TGTCCACAGG TGACAACTGG AACGGCATTA TGAAGGACAC CCTCCGGGAC 
TGTGACCAGG AGTCCACATG CTACAATACA GTCATCTCCC CCATCTACTT CGTGTCCTTC 
GTGCTGACGG CCCAGTTTGT GCTGGTCAAC GTGGTGATCG CTGTGCTGAT GAAGCACCTG 
GAGGAAAGCA ACAAGGAGGC CAAAGAGGAG GCCGAGCTGG AGGCTGAGCT GGAGCTGGAG 
ATGAAGACGC TCAGCCCACA GCCCCACTCG CCACTGGGCA GCCCCTTCCT CTGGCCGGGG 
GTCGAGGGCC CCGACAGCCC CGATAGCCCC AAGCTCAGGG CCCCGCACAC CGCGGCCCAC 
ACGGGAGCAG CCTCGCGCTT CTCCCTGGAA CACCCCACGG ACAGACAGCT GTTTGACACC 
ATATCCCTGC TGATCCAGGG CTCCCTGGAA GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT CCCGCCCGCA GCCTGGGCGG CTCCGACCCA 
CAGATCCCTC TAGCTGAGAT GGAGGCTCTG TCTCTGACGT CAGAGATTGT GTCTGAACCG 
TCCCACTCTC TAGCTCTGAC GGATGACTCT TTGCCTGATG ACACTCACAC ACTCTTACCT 
AGTGCCCTGG ACAGCAATAT GGAGCCCCAC CCCGAGGAGA TGCCCCTTGC ACTGGGACTG 
GACCTGCTGA CTGTGAGGAA GTCTGGGGTC AGCCGGACAC ACTCCCTGCC CAATGACAGC 
TACATGTTCC GGGACGGAGG CACCGCTGAG GGGTCCCTAG GATGCAGAAG CTGGGGGCTC 
CCCAAAGCTC AGTCAGGCTC TGTCTTATCT GTCCACTCGC AGCCAGCAGA CACCAGCTAC 
ATCCTGCAGC TTCCCAAAGA CACACCTCAC CTGCTCCAGC CCCACAGCGC TCCCACCTGG 
GGCACCATTC CCAAACTGCC CCCACCAGGC CGCTCCCCCT TGGCTCAGAG GCCACTCAGG 
CGCCAGGCAG CAATAAGGAC TGATTCTCTG GACGTTCAGG GCCTGGGCAG CCGGGAAGAC 
CTGCTGTCCG AGGTGAGTGG GCCCTCCCCA CCTCTGGCCC GGGCCTCCTC CTTCTGGGGC 
CACTTGGGCG CCCAGATGCA GCAGCATCCC CACCGCCAGA GCAAGATCTC GAAGCACATG 
CCCCCGCCAG CCCCCCGCCC AGGCTCAGAA ACCACCTGGA GCAAAGACCC ACCCGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGACCGTG 
GGCAGCCAGG AGGAGCCCCC GTCCCCACGG GACCTGAAGA AGTGTCACAG CGTGGAGGCC 
CAGAGCTGCC GGCGCAGGCC TGCGTCCTGG CTGGATGAAC AGAGGAGACA CTCCATCGCG 
GTCAGCTGCC TGGACGGCGG CTCCCAACCC AGCCTGGGCC CCGGCCCCTC TAGCCTTGGC 
GGCCAGCCTC TTGGGGGACC TGGAAGCCGG CCCAAGAAAA AACTCAGCCC ACCTAGTATC 
TCCATAGACC CCCCAGAGAG TCAGGGCCCT CGGCCCCCAC CCAGCCCCGG CGTCTGCCTC 
CGGCGGAGGG CTCCGTCCAG CGACTCCAGG GACCCCTCGG CCTCCGGCCC CCCTGACAGC 
ATGGCTGCCT CGCCCCCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT AGCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624654.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624711.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X13, mRNA.

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CloneID	OCe11919
Clone ID Related Accession (Same CDS sequence)	XM_005624654.2
Accession Version	XM_005624654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGAGGCCCA GTGTAAACCC 
TACTACTCAG ACTACTCCCG ATTCCGGCTC CTCGTCCACC ACTTGTGTAC CAGCCACTAT 
CTGGACCTCT TCATCACGGG TGTCATTGGT CTGAATGTGG TCACCATGGC CATGGAGCAC 
TACCAGCAGC CCCAGATCCT GGACGAGGCC TTGAAGATCT GCAACTATAT CTTCACCGTC 
ATCTTTGTCT TGGAGTCAGT TTTTAAACTC GTGGCCTTTG GCTTCCGTCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCCATC GTGCTGCTGT CCATCATGGG CATCACGCTG 
GAGGAGATTG AGGTCAATGC CTCGCTGCCC ATCAACCCCA CCATCATCCG CATCATGAGA 
GTGCTGCGCA TTGCCCGAGT GCTGAAGCTG CTGAAGATGG CTGTGGGCAT GCGGGCGCTG 
CTGGACACAG TCATGCAGGC CCTGCCTCAG GTGGGGAACC TGGGACTTCT CTTCATGTTG 
TTGTTTTTCA TCTTTGCAGC TCTGGGTGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACGCACCCCT GCGAGGGCCT GGGCCGGCAT GCCACCTTTC GGAACTTTGG CATGGCCTTC 
CTGACCCTCT TCCGAGTGTC CACAGGTGAC AACTGGAACG GCATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACATGCTAC AATACAGTCA TCTCCCCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TGATCGCTGT GCTGATGAAG 
CACCTGGAGG AAAGCAACAA GGAGGCCAAA GAGGAGGCCG AGCTGGAGGC TGAGCTGGAG 
CTGGAGATGA AGACGCTCAG CCCACAGCCC CACTCGCCAC TGGGCAGCCC CTTCCTCTGG 
CCGGGGGTCG AGGGCCCCGA CAGCCCCGAT AGCCCCAAGC TCAGGGCCCC GCACACCGCG 
GCCCACACGG GAGCAGCCTC GCGCTTCTCC CTGGAACACC CCACGGACAG ACAGCTGTTT 
GACACCATAT CCCTGCTGAT CCAGGGCTCC CTGGAAGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTCCCG CCCGCAGCCT GGGCGGCTCC 
GACCCACAGA TGGAGCCCCA CCCCGAGGAG ATGCCCCTTG CACTGGGACT GGACCTGCTG 
ACTGTGAGGA AGTCTGGGGT CAGCCGGACA CACTCCCTGC CCAATGACAG CTACATGTTC 
CGGGACGGAG GCACCGCTGA GGGGTCCCTA GGATGCAGAA GCTGGGGGCT CCCCAAAGCT 
CAGTCAGGCT CTGTCTTATC TGTCCACTCG CAGCCAGCAG ACACCAGCTA CATCCTGCAG 
CTTCCCAAAG ACACACCTCA CCTGCTCCAG CCCCACAGCG CTCCCACCTG GGGCACCATT 
CCCAAACTGC CCCCACCAGG CCGCTCCCCC TTGGCTCAGA GGCCACTCAG GCGCCAGGCA 
GCAATAAGGA CTGATTCTCT GGACGTTCAG GGCCTGGGCA GCCGGGAAGA CCTGCTGTCC 
GAGGTGAGTG GGCCCTCCCC ACCTCTGGCC CGGGCCTCCT CCTTCTGGGG CCACTTGGGC 
GCCCAGATGC AGCAGCATCC CCACCGCCAG AGCAAGATCT CGAAGCACAT GCCCCCGCCA 
GCCCCCCGCC CAGGCTCAGA AACCACCTGG AGCAAAGACC CACCCGAGAC CAGAAGCAGC 
TTAGAGCTGG ACACGGAGCT GAGCTGGATT TCAGGAGACC TCCTGACCGT GGGCAGCCAG 
GAGGAGCCCC CGTCCCCACG GGACCTGAAG AAGTGTCACA GCGTGGAGGC CCAGAGCTGC 
CGGCGCAGGC CTGCGTCCTG GCTGGATGAA CAGAGGAGAC ACTCCATCGC GGTCAGCTGC 
CTGGACGGCG GCTCCCAACC CAGCCTGGGC CCCGGCCCCT CTAGCCTTGG CGGCCAGCCT 
CTTGGGGGAC CTGGAAGCCG GCCCAAGAAA AAACTCAGCC CACCTAGTAT CTCCATAGAC 
CCCCCAGAGA GTCAGGGCCC TCGGCCCCCA CCCAGCCCCG GCGTCTGCCT CCGGCGGAGG 
GCTCCGTCCA GCGACTCCAG GGACCCCTCG GCCTCCGGCC CCCCTGACAG CATGGCTGCC 
TCGCCCCCCC CAAAGAAAGA CGTGCTGAGT CTCTCTGGTT TAGCCTCTGA CCCAGCAGAC 
CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624711.1
CDS	527..7558
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624654.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624654.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGAGGCCCA GTGTAAACCC 
TACTACTCAG ACTACTCCCG ATTCCGGCTC CTCGTCCACC ACTTGTGTAC CAGCCACTAT 
CTGGACCTCT TCATCACGGG TGTCATTGGT CTGAATGTGG TCACCATGGC CATGGAGCAC 
TACCAGCAGC CCCAGATCCT GGACGAGGCC TTGAAGATCT GCAACTATAT CTTCACCGTC 
ATCTTTGTCT TGGAGTCAGT TTTTAAACTC GTGGCCTTTG GCTTCCGTCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCCATC GTGCTGCTGT CCATCATGGG CATCACGCTG 
GAGGAGATTG AGGTCAATGC CTCGCTGCCC ATCAACCCCA CCATCATCCG CATCATGAGA 
GTGCTGCGCA TTGCCCGAGT GCTGAAGCTG CTGAAGATGG CTGTGGGCAT GCGGGCGCTG 
CTGGACACAG TCATGCAGGC CCTGCCTCAG GTGGGGAACC TGGGACTTCT CTTCATGTTG 
TTGTTTTTCA TCTTTGCAGC TCTGGGTGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACGCACCCCT GCGAGGGCCT GGGCCGGCAT GCCACCTTTC GGAACTTTGG CATGGCCTTC 
CTGACCCTCT TCCGAGTGTC CACAGGTGAC AACTGGAACG GCATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACATGCTAC AATACAGTCA TCTCCCCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TGATCGCTGT GCTGATGAAG 
CACCTGGAGG AAAGCAACAA GGAGGCCAAA GAGGAGGCCG AGCTGGAGGC TGAGCTGGAG 
CTGGAGATGA AGACGCTCAG CCCACAGCCC CACTCGCCAC TGGGCAGCCC CTTCCTCTGG 
CCGGGGGTCG AGGGCCCCGA CAGCCCCGAT AGCCCCAAGC TCAGGGCCCC GCACACCGCG 
GCCCACACGG GAGCAGCCTC GCGCTTCTCC CTGGAACACC CCACGGACAG ACAGCTGTTT 
GACACCATAT CCCTGCTGAT CCAGGGCTCC CTGGAAGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTCCCG CCCGCAGCCT GGGCGGCTCC 
GACCCACAGA TGGAGCCCCA CCCCGAGGAG ATGCCCCTTG CACTGGGACT GGACCTGCTG 
ACTGTGAGGA AGTCTGGGGT CAGCCGGACA CACTCCCTGC CCAATGACAG CTACATGTTC 
CGGGACGGAG GCACCGCTGA GGGGTCCCTA GGATGCAGAA GCTGGGGGCT CCCCAAAGCT 
CAGTCAGGCT CTGTCTTATC TGTCCACTCG CAGCCAGCAG ACACCAGCTA CATCCTGCAG 
CTTCCCAAAG ACACACCTCA CCTGCTCCAG CCCCACAGCG CTCCCACCTG GGGCACCATT 
CCCAAACTGC CCCCACCAGG CCGCTCCCCC TTGGCTCAGA GGCCACTCAG GCGCCAGGCA 
GCAATAAGGA CTGATTCTCT GGACGTTCAG GGCCTGGGCA GCCGGGAAGA CCTGCTGTCC 
GAGGTGAGTG GGCCCTCCCC ACCTCTGGCC CGGGCCTCCT CCTTCTGGGG CCACTTGGGC 
GCCCAGATGC AGCAGCATCC CCACCGCCAG AGCAAGATCT CGAAGCACAT GCCCCCGCCA 
GCCCCCCGCC CAGGCTCAGA AACCACCTGG AGCAAAGACC CACCCGAGAC CAGAAGCAGC 
TTAGAGCTGG ACACGGAGCT GAGCTGGATT TCAGGAGACC TCCTGACCGT GGGCAGCCAG 
GAGGAGCCCC CGTCCCCACG GGACCTGAAG AAGTGTCACA GCGTGGAGGC CCAGAGCTGC 
CGGCGCAGGC CTGCGTCCTG GCTGGATGAA CAGAGGAGAC ACTCCATCGC GGTCAGCTGC 
CTGGACGGCG GCTCCCAACC CAGCCTGGGC CCCGGCCCCT CTAGCCTTGG CGGCCAGCCT 
CTTGGGGGAC CTGGAAGCCG GCCCAAGAAA AAACTCAGCC CACCTAGTAT CTCCATAGAC 
CCCCCAGAGA GTCAGGGCCC TCGGCCCCCA CCCAGCCCCG GCGTCTGCCT CCGGCGGAGG 
GCTCCGTCCA GCGACTCCAG GGACCCCTCG GCCTCCGGCC CCCCTGACAG CATGGCTGCC 
TCGCCCCCCC CAAAGAAAGA CGTGCTGAGT CTCTCTGGTT TAGCCTCTGA CCCAGCAGAC 
CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860640.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865733.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X11, mRNA.

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CloneID	OCe11920
Clone ID Related Accession (Same CDS sequence)	XM_860640.5 , XM_860640.4
Accession Version	XM_860640.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_860640.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG 
GGCTCCCTGG AAGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC GGCTCCGACC CACAGATGGA GCCCCACCCC 
GAGGAGATGC CCCTTGCACT GGGACTGGAC CTGCTGACTG TGAGGAAGTC TGGGGTCAGC 
CGGACACACT CCCTGCCCAA TGACAGCTAC ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG 
TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC AAAGCTCAGT CAGGCTCTGT CTTATCTGTC 
CACTCGCAGC CAGCAGACAC CAGCTACATC CTGCAGCTTC CCAAAGACAC ACCTCACCTG 
CTCCAGCCCC ACAGCGCTCC CACCTGGGGC ACCATTCCCA AACTGCCCCC ACCAGGCCGC 
TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TAAGGACTGA TTCTCTGGAC 
GTTCAGGGCC TGGGCAGCCG GGAAGACCTG CTGTCCGAGG TGAGTGGGCC CTCCCCACCT 
CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC TTGGGCGCCC AGATGCAGCA GCATCCCCAC 
CGCCAGAGCA AGATCTCGAA GCACATGCCC CCGCCAGCCC CCCGCCCAGG CTCAGAAACC 
ACCTGGAGCA AAGACCCACC CGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GACCGTGGGC AGCCAGGAGG AGCCCCCGTC CCCACGGGAC 
CTGAAGAAGT GTCACAGCGT GGAGGCCCAG AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG 
GATGAACAGA GGAGACACTC CATCGCGGTC AGCTGCCTGG ACGGCGGCTC CCAACCCAGC 
CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC CAGCCTCTTG GGGGACCTGG AAGCCGGCCC 
AAGAAAAAAC TCAGCCCACC TAGTATCTCC ATAGACCCCC CAGAGAGTCA GGGCCCTCGG 
CCCCCACCCA GCCCCGGCGT CTGCCTCCGG CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC 
CCCTCGGCCT CCGGCCCCCC TGACAGCATG GCTGCCTCGC CCCCCCCAAA GAAAGACGTG 
CTGAGTCTCT CTGGTTTAGC CTCTGACCCA GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865733.1
CDS	504..7511
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860640.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860640.5

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG 
GGCTCCCTGG AAGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC GGCTCCGACC CACAGATGGA GCCCCACCCC 
GAGGAGATGC CCCTTGCACT GGGACTGGAC CTGCTGACTG TGAGGAAGTC TGGGGTCAGC 
CGGACACACT CCCTGCCCAA TGACAGCTAC ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG 
TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC AAAGCTCAGT CAGGCTCTGT CTTATCTGTC 
CACTCGCAGC CAGCAGACAC CAGCTACATC CTGCAGCTTC CCAAAGACAC ACCTCACCTG 
CTCCAGCCCC ACAGCGCTCC CACCTGGGGC ACCATTCCCA AACTGCCCCC ACCAGGCCGC 
TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TAAGGACTGA TTCTCTGGAC 
GTTCAGGGCC TGGGCAGCCG GGAAGACCTG CTGTCCGAGG TGAGTGGGCC CTCCCCACCT 
CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC TTGGGCGCCC AGATGCAGCA GCATCCCCAC 
CGCCAGAGCA AGATCTCGAA GCACATGCCC CCGCCAGCCC CCCGCCCAGG CTCAGAAACC 
ACCTGGAGCA AAGACCCACC CGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GACCGTGGGC AGCCAGGAGG AGCCCCCGTC CCCACGGGAC 
CTGAAGAAGT GTCACAGCGT GGAGGCCCAG AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG 
GATGAACAGA GGAGACACTC CATCGCGGTC AGCTGCCTGG ACGGCGGCTC CCAACCCAGC 
CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC CAGCCTCTTG GGGGACCTGG AAGCCGGCCC 
AAGAAAAAAC TCAGCCCACC TAGTATCTCC ATAGACCCCC CAGAGAGTCA GGGCCCTCGG 
CCCCCACCCA GCCCCGGCGT CTGCCTCCGG CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC 
CCCTCGGCCT CCGGCCCCCC TGACAGCATG GCTGCCTCGC CCCCCCCAAA GAAAGACGTG 
CTGAGTCTCT CTGGTTTAGC CTCTGACCCA GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860640.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865733.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X15, mRNA.

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CloneID	OCe11920
Clone ID Related Accession (Same CDS sequence)	XM_860640.5 , XM_860640.4
Accession Version	XM_860640.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_860640.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG 
GGCTCCCTGG AAGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC GGCTCCGACC CACAGATGGA GCCCCACCCC 
GAGGAGATGC CCCTTGCACT GGGACTGGAC CTGCTGACTG TGAGGAAGTC TGGGGTCAGC 
CGGACACACT CCCTGCCCAA TGACAGCTAC ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG 
TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC AAAGCTCAGT CAGGCTCTGT CTTATCTGTC 
CACTCGCAGC CAGCAGACAC CAGCTACATC CTGCAGCTTC CCAAAGACAC ACCTCACCTG 
CTCCAGCCCC ACAGCGCTCC CACCTGGGGC ACCATTCCCA AACTGCCCCC ACCAGGCCGC 
TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TAAGGACTGA TTCTCTGGAC 
GTTCAGGGCC TGGGCAGCCG GGAAGACCTG CTGTCCGAGG TGAGTGGGCC CTCCCCACCT 
CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC TTGGGCGCCC AGATGCAGCA GCATCCCCAC 
CGCCAGAGCA AGATCTCGAA GCACATGCCC CCGCCAGCCC CCCGCCCAGG CTCAGAAACC 
ACCTGGAGCA AAGACCCACC CGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GACCGTGGGC AGCCAGGAGG AGCCCCCGTC CCCACGGGAC 
CTGAAGAAGT GTCACAGCGT GGAGGCCCAG AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG 
GATGAACAGA GGAGACACTC CATCGCGGTC AGCTGCCTGG ACGGCGGCTC CCAACCCAGC 
CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC CAGCCTCTTG GGGGACCTGG AAGCCGGCCC 
AAGAAAAAAC TCAGCCCACC TAGTATCTCC ATAGACCCCC CAGAGAGTCA GGGCCCTCGG 
CCCCCACCCA GCCCCGGCGT CTGCCTCCGG CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC 
CCCTCGGCCT CCGGCCCCCC TGACAGCATG GCTGCCTCGC CCCCCCCAAA GAAAGACGTG 
CTGAGTCTCT CTGGTTTAGC CTCTGACCCA GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865733.1
CDS	527..7534
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860640.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860640.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG 
GGCTCCCTGG AAGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC GGCTCCGACC CACAGATGGA GCCCCACCCC 
GAGGAGATGC CCCTTGCACT GGGACTGGAC CTGCTGACTG TGAGGAAGTC TGGGGTCAGC 
CGGACACACT CCCTGCCCAA TGACAGCTAC ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG 
TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC AAAGCTCAGT CAGGCTCTGT CTTATCTGTC 
CACTCGCAGC CAGCAGACAC CAGCTACATC CTGCAGCTTC CCAAAGACAC ACCTCACCTG 
CTCCAGCCCC ACAGCGCTCC CACCTGGGGC ACCATTCCCA AACTGCCCCC ACCAGGCCGC 
TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TAAGGACTGA TTCTCTGGAC 
GTTCAGGGCC TGGGCAGCCG GGAAGACCTG CTGTCCGAGG TGAGTGGGCC CTCCCCACCT 
CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC TTGGGCGCCC AGATGCAGCA GCATCCCCAC 
CGCCAGAGCA AGATCTCGAA GCACATGCCC CCGCCAGCCC CCCGCCCAGG CTCAGAAACC 
ACCTGGAGCA AAGACCCACC CGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GACCGTGGGC AGCCAGGAGG AGCCCCCGTC CCCACGGGAC 
CTGAAGAAGT GTCACAGCGT GGAGGCCCAG AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG 
GATGAACAGA GGAGACACTC CATCGCGGTC AGCTGCCTGG ACGGCGGCTC CCAACCCAGC 
CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC CAGCCTCTTG GGGGACCTGG AAGCCGGCCC 
AAGAAAAAAC TCAGCCCACC TAGTATCTCC ATAGACCCCC CAGAGAGTCA GGGCCCTCGG 
CCCCCACCCA GCCCCGGCGT CTGCCTCCGG CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC 
CCCTCGGCCT CCGGCCCCCC TGACAGCATG GCTGCCTCGC CCCCCCCAAA GAAAGACGTG 
CTGAGTCTCT CTGGTTTAGC CTCTGACCCA GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860625.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865718.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X17, mRNA.

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CloneID	OCe11921
Clone ID Related Accession (Same CDS sequence)	XM_860625.4 , XM_860625.5
Accession Version	XM_860625.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X17
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_860625.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGAGGC CCAGTGTAAA 
CCCTACTACT CAGACTACTC CCGATTCCGG CTCCTCGTCC ACCACTTGTG TACCAGCCAC 
TATCTGGACC TCTTCATCAC GGGTGTCATT GGTCTGAATG TGGTCACCAT GGCCATGGAG 
CACTACCAGC AGCCCCAGAT CCTGGACGAG GCCTTGAAGA TCTGCAACTA TATCTTCACC 
GTCATCTTTG TCTTGGAGTC AGTTTTTAAA CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCC ATCGTGCTGC TGTCCATCAT GGGCATCACG 
CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG CCCATCAACC CCACCATCAT CCGCATCATG 
AGAGTGCTGC GCATTGCCCG AGTGCTGAAG CTGCTGAAGA TGGCTGTGGG CATGCGGGCG 
CTGCTGGACA CAGTCATGCA GGCCCTGCCT CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
TTGTTGTTTT TCATCTTTGC AGCTCTGGGT GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACGCACC CCTGCGAGGG CCTGGGCCGG CATGCCACCT TTCGGAACTT TGGCATGGCC 
TTCCTGACCC TCTTCCGAGT GTCCACAGGT GACAACTGGA ACGGCATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACATGC TACAATACAG TCATCTCCCC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTGATCGC TGTGCTGATG 
AAGCACCTGG AGGAAAGCAA CAAGGAGGCC AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG 
GAGCTGGAGA TGAAGACGCT CAGCCCACAG CCCCACTCGC CACTGGGCAG CCCCTTCCTC 
TGGCCGGGGG TCGAGGGCCC CGACAGCCCC GATAGCCCCA AGCTCAGGGC CCCGCACACC 
GCGGCCCACA CGGGAGCAGC CTCGCGCTTC TCCCTGGAAC ACCCCACGGA CAGACAGCTG 
TTTGACACCA TATCCCTGCT GATCCAGGGC TCCCTGGAAG GGGAGCTGAA GCTGATGGAC 
GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT GCCTTCCCTC CCGCCCGCAG CCTGGGCGGC 
TCCGACCCAC AGATGGAGCC CCACCCCGAG GAGATGCCCC TTGCACTGGG ACTGGACCTG 
CTGACTGTGA GGAAGTCTGG GGTCAGCCGG ACACACTCCC TGCCCAATGA CAGCTACATG 
TTCCGGGACG GAGGCACCGC TGAGGGGTCC CTAGGATGCA GAAGCTGGGG GCTCCCCAAA 
GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC TCGCAGCCAG CAGACACCAG CTACATCCTG 
CAGCTTCCCA AAGACACACC TCACCTGCTC CAGCCCCACA GCGCTCCCAC CTGGGGCACC 
ATTCCCAAAC TGCCCCCACC AGGCCGCTCC CCCTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAA GGACTGATTC TCTGGACGTT CAGGGCCTGG GCAGCCGGGA AGACCTGCTG 
TCCGAGGTGA GTGGGCCCTC CCCACCTCTG GCCCGGGCCT CCTCCTTCTG GGGCCACTTG 
GGCGCCCAGA TGCAGCAGCA TCCCCACCGC CAGAGCAAGA TCTCGAAGCA CATGCCCCCG 
CCAGCCCCCC GCCCAGGCTC AGAAACCACC TGGAGCAAAG ACCCACCCGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC 
CAGGAGGAGC CCCCGTCCCC ACGGGACCTG AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT GAACAGAGGA GACACTCCAT CGCGGTCAGC 
TGCCTGGACG GCGGCTCCCA ACCCAGCCTG GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG 
CCTCTTGGGG GACCTGGAAG CCGGCCCAAG AAAAAACTCA GCCCACCTAG TATCTCCATA 
GACCCCCCAG AGAGTCAGGG CCCTCGGCCC CCACCCAGCC CCGGCGTCTG CCTCCGGCGG 
AGGGCTCCGT CCAGCGACTC CAGGGACCCC TCGGCCTCCG GCCCCCCTGA CAGCATGGCT 
GCCTCGCCCC CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTAGCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865718.1
CDS	527..7501
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860625.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860625.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGAGGC CCAGTGTAAA 
CCCTACTACT CAGACTACTC CCGATTCCGG CTCCTCGTCC ACCACTTGTG TACCAGCCAC 
TATCTGGACC TCTTCATCAC GGGTGTCATT GGTCTGAATG TGGTCACCAT GGCCATGGAG 
CACTACCAGC AGCCCCAGAT CCTGGACGAG GCCTTGAAGA TCTGCAACTA TATCTTCACC 
GTCATCTTTG TCTTGGAGTC AGTTTTTAAA CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCC ATCGTGCTGC TGTCCATCAT GGGCATCACG 
CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG CCCATCAACC CCACCATCAT CCGCATCATG 
AGAGTGCTGC GCATTGCCCG AGTGCTGAAG CTGCTGAAGA TGGCTGTGGG CATGCGGGCG 
CTGCTGGACA CAGTCATGCA GGCCCTGCCT CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
TTGTTGTTTT TCATCTTTGC AGCTCTGGGT GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACGCACC CCTGCGAGGG CCTGGGCCGG CATGCCACCT TTCGGAACTT TGGCATGGCC 
TTCCTGACCC TCTTCCGAGT GTCCACAGGT GACAACTGGA ACGGCATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACATGC TACAATACAG TCATCTCCCC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTGATCGC TGTGCTGATG 
AAGCACCTGG AGGAAAGCAA CAAGGAGGCC AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG 
GAGCTGGAGA TGAAGACGCT CAGCCCACAG CCCCACTCGC CACTGGGCAG CCCCTTCCTC 
TGGCCGGGGG TCGAGGGCCC CGACAGCCCC GATAGCCCCA AGCTCAGGGC CCCGCACACC 
GCGGCCCACA CGGGAGCAGC CTCGCGCTTC TCCCTGGAAC ACCCCACGGA CAGACAGCTG 
TTTGACACCA TATCCCTGCT GATCCAGGGC TCCCTGGAAG GGGAGCTGAA GCTGATGGAC 
GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT GCCTTCCCTC CCGCCCGCAG CCTGGGCGGC 
TCCGACCCAC AGATGGAGCC CCACCCCGAG GAGATGCCCC TTGCACTGGG ACTGGACCTG 
CTGACTGTGA GGAAGTCTGG GGTCAGCCGG ACACACTCCC TGCCCAATGA CAGCTACATG 
TTCCGGGACG GAGGCACCGC TGAGGGGTCC CTAGGATGCA GAAGCTGGGG GCTCCCCAAA 
GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC TCGCAGCCAG CAGACACCAG CTACATCCTG 
CAGCTTCCCA AAGACACACC TCACCTGCTC CAGCCCCACA GCGCTCCCAC CTGGGGCACC 
ATTCCCAAAC TGCCCCCACC AGGCCGCTCC CCCTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAA GGACTGATTC TCTGGACGTT CAGGGCCTGG GCAGCCGGGA AGACCTGCTG 
TCCGAGGTGA GTGGGCCCTC CCCACCTCTG GCCCGGGCCT CCTCCTTCTG GGGCCACTTG 
GGCGCCCAGA TGCAGCAGCA TCCCCACCGC CAGAGCAAGA TCTCGAAGCA CATGCCCCCG 
CCAGCCCCCC GCCCAGGCTC AGAAACCACC TGGAGCAAAG ACCCACCCGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC 
CAGGAGGAGC CCCCGTCCCC ACGGGACCTG AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT GAACAGAGGA GACACTCCAT CGCGGTCAGC 
TGCCTGGACG GCGGCTCCCA ACCCAGCCTG GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG 
CCTCTTGGGG GACCTGGAAG CCGGCCCAAG AAAAAACTCA GCCCACCTAG TATCTCCATA 
GACCCCCCAG AGAGTCAGGG CCCTCGGCCC CCACCCAGCC CCGGCGTCTG CCTCCGGCGG 
AGGGCTCCGT CCAGCGACTC CAGGGACCCC TCGGCCTCCG GCCCCCCTGA CAGCATGGCT 
GCCTCGCCCC CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTAGCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860625.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865718.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X14, mRNA.

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CloneID	OCe11921
Clone ID Related Accession (Same CDS sequence)	XM_860625.4 , XM_860625.5
Accession Version	XM_860625.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_860625.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGAGGC CCAGTGTAAA 
CCCTACTACT CAGACTACTC CCGATTCCGG CTCCTCGTCC ACCACTTGTG TACCAGCCAC 
TATCTGGACC TCTTCATCAC GGGTGTCATT GGTCTGAATG TGGTCACCAT GGCCATGGAG 
CACTACCAGC AGCCCCAGAT CCTGGACGAG GCCTTGAAGA TCTGCAACTA TATCTTCACC 
GTCATCTTTG TCTTGGAGTC AGTTTTTAAA CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCC ATCGTGCTGC TGTCCATCAT GGGCATCACG 
CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG CCCATCAACC CCACCATCAT CCGCATCATG 
AGAGTGCTGC GCATTGCCCG AGTGCTGAAG CTGCTGAAGA TGGCTGTGGG CATGCGGGCG 
CTGCTGGACA CAGTCATGCA GGCCCTGCCT CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
TTGTTGTTTT TCATCTTTGC AGCTCTGGGT GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACGCACC CCTGCGAGGG CCTGGGCCGG CATGCCACCT TTCGGAACTT TGGCATGGCC 
TTCCTGACCC TCTTCCGAGT GTCCACAGGT GACAACTGGA ACGGCATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACATGC TACAATACAG TCATCTCCCC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTGATCGC TGTGCTGATG 
AAGCACCTGG AGGAAAGCAA CAAGGAGGCC AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG 
GAGCTGGAGA TGAAGACGCT CAGCCCACAG CCCCACTCGC CACTGGGCAG CCCCTTCCTC 
TGGCCGGGGG TCGAGGGCCC CGACAGCCCC GATAGCCCCA AGCTCAGGGC CCCGCACACC 
GCGGCCCACA CGGGAGCAGC CTCGCGCTTC TCCCTGGAAC ACCCCACGGA CAGACAGCTG 
TTTGACACCA TATCCCTGCT GATCCAGGGC TCCCTGGAAG GGGAGCTGAA GCTGATGGAC 
GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT GCCTTCCCTC CCGCCCGCAG CCTGGGCGGC 
TCCGACCCAC AGATGGAGCC CCACCCCGAG GAGATGCCCC TTGCACTGGG ACTGGACCTG 
CTGACTGTGA GGAAGTCTGG GGTCAGCCGG ACACACTCCC TGCCCAATGA CAGCTACATG 
TTCCGGGACG GAGGCACCGC TGAGGGGTCC CTAGGATGCA GAAGCTGGGG GCTCCCCAAA 
GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC TCGCAGCCAG CAGACACCAG CTACATCCTG 
CAGCTTCCCA AAGACACACC TCACCTGCTC CAGCCCCACA GCGCTCCCAC CTGGGGCACC 
ATTCCCAAAC TGCCCCCACC AGGCCGCTCC CCCTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAA GGACTGATTC TCTGGACGTT CAGGGCCTGG GCAGCCGGGA AGACCTGCTG 
TCCGAGGTGA GTGGGCCCTC CCCACCTCTG GCCCGGGCCT CCTCCTTCTG GGGCCACTTG 
GGCGCCCAGA TGCAGCAGCA TCCCCACCGC CAGAGCAAGA TCTCGAAGCA CATGCCCCCG 
CCAGCCCCCC GCCCAGGCTC AGAAACCACC TGGAGCAAAG ACCCACCCGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC 
CAGGAGGAGC CCCCGTCCCC ACGGGACCTG AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT GAACAGAGGA GACACTCCAT CGCGGTCAGC 
TGCCTGGACG GCGGCTCCCA ACCCAGCCTG GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG 
CCTCTTGGGG GACCTGGAAG CCGGCCCAAG AAAAAACTCA GCCCACCTAG TATCTCCATA 
GACCCCCCAG AGAGTCAGGG CCCTCGGCCC CCACCCAGCC CCGGCGTCTG CCTCCGGCGG 
AGGGCTCCGT CCAGCGACTC CAGGGACCCC TCGGCCTCCG GCCCCCCTGA CAGCATGGCT 
GCCTCGCCCC CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTAGCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865718.1
CDS	504..7478
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860625.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860625.5

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGAGGC CCAGTGTAAA 
CCCTACTACT CAGACTACTC CCGATTCCGG CTCCTCGTCC ACCACTTGTG TACCAGCCAC 
TATCTGGACC TCTTCATCAC GGGTGTCATT GGTCTGAATG TGGTCACCAT GGCCATGGAG 
CACTACCAGC AGCCCCAGAT CCTGGACGAG GCCTTGAAGA TCTGCAACTA TATCTTCACC 
GTCATCTTTG TCTTGGAGTC AGTTTTTAAA CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCC ATCGTGCTGC TGTCCATCAT GGGCATCACG 
CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG CCCATCAACC CCACCATCAT CCGCATCATG 
AGAGTGCTGC GCATTGCCCG AGTGCTGAAG CTGCTGAAGA TGGCTGTGGG CATGCGGGCG 
CTGCTGGACA CAGTCATGCA GGCCCTGCCT CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
TTGTTGTTTT TCATCTTTGC AGCTCTGGGT GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACGCACC CCTGCGAGGG CCTGGGCCGG CATGCCACCT TTCGGAACTT TGGCATGGCC 
TTCCTGACCC TCTTCCGAGT GTCCACAGGT GACAACTGGA ACGGCATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACATGC TACAATACAG TCATCTCCCC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTGATCGC TGTGCTGATG 
AAGCACCTGG AGGAAAGCAA CAAGGAGGCC AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG 
GAGCTGGAGA TGAAGACGCT CAGCCCACAG CCCCACTCGC CACTGGGCAG CCCCTTCCTC 
TGGCCGGGGG TCGAGGGCCC CGACAGCCCC GATAGCCCCA AGCTCAGGGC CCCGCACACC 
GCGGCCCACA CGGGAGCAGC CTCGCGCTTC TCCCTGGAAC ACCCCACGGA CAGACAGCTG 
TTTGACACCA TATCCCTGCT GATCCAGGGC TCCCTGGAAG GGGAGCTGAA GCTGATGGAC 
GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT GCCTTCCCTC CCGCCCGCAG CCTGGGCGGC 
TCCGACCCAC AGATGGAGCC CCACCCCGAG GAGATGCCCC TTGCACTGGG ACTGGACCTG 
CTGACTGTGA GGAAGTCTGG GGTCAGCCGG ACACACTCCC TGCCCAATGA CAGCTACATG 
TTCCGGGACG GAGGCACCGC TGAGGGGTCC CTAGGATGCA GAAGCTGGGG GCTCCCCAAA 
GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC TCGCAGCCAG CAGACACCAG CTACATCCTG 
CAGCTTCCCA AAGACACACC TCACCTGCTC CAGCCCCACA GCGCTCCCAC CTGGGGCACC 
ATTCCCAAAC TGCCCCCACC AGGCCGCTCC CCCTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAA GGACTGATTC TCTGGACGTT CAGGGCCTGG GCAGCCGGGA AGACCTGCTG 
TCCGAGGTGA GTGGGCCCTC CCCACCTCTG GCCCGGGCCT CCTCCTTCTG GGGCCACTTG 
GGCGCCCAGA TGCAGCAGCA TCCCCACCGC CAGAGCAAGA TCTCGAAGCA CATGCCCCCG 
CCAGCCCCCC GCCCAGGCTC AGAAACCACC TGGAGCAAAG ACCCACCCGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC 
CAGGAGGAGC CCCCGTCCCC ACGGGACCTG AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT GAACAGAGGA GACACTCCAT CGCGGTCAGC 
TGCCTGGACG GCGGCTCCCA ACCCAGCCTG GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG 
CCTCTTGGGG GACCTGGAAG CCGGCCCAAG AAAAAACTCA GCCCACCTAG TATCTCCATA 
GACCCCCCAG AGAGTCAGGG CCCTCGGCCC CCACCCAGCC CCGGCGTCTG CCTCCGGCGG 
AGGGCTCCGT CCAGCGACTC CAGGGACCCC TCGGCCTCCG GCCCCCCTGA CAGCATGGCT 
GCCTCGCCCC CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTAGCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624655.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624712.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X15, mRNA.

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CloneID	OCe11922
Clone ID Related Accession (Same CDS sequence)	XM_005624655.3 , XM_005624655.2
Accession Version	XM_005624655.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624655.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAAGGCC CAGTGTAAAC CCTACTACTC AGACTACTCC 
CGATTCCGGC TCCTCGTCCA CCACTTGTGT ACCAGCCACT ATCTGGACCT CTTCATCACG 
GGTGTCATTG GTCTGAATGT GGTCACCATG GCCATGGAGC ACTACCAGCA GCCCCAGATC 
CTGGACGAGG CCTTGAAGAT CTGCAACTAT ATCTTCACCG TCATCTTTGT CTTGGAGTCA 
GTTTTTAAAC TCGTGGCCTT TGGCTTCCGT CGGTTCTTCC AGGACAGGTG GAACCAGCTG 
GACCTGGCCA TCGTGCTGCT GTCCATCATG GGCATCACGC TGGAGGAGAT TGAGGTCAAT 
GCCTCGCTGC CCATCAACCC CACCATCATC CGCATCATGA GAGTGCTGCG CATTGCCCGA 
GTGCTGAAGC TGCTGAAGAT GGCTGTGGGC ATGCGGGCGC TGCTGGACAC AGTCATGCAG 
GCCCTGCCTC AGGTGGGGAA CCTGGGACTT CTCTTCATGT TGTTGTTTTT CATCTTTGCA 
GCTCTGGGTG TGGAGCTCTT TGGAGACCTG GAGTGTGATG AGACGCACCC CTGCGAGGGC 
CTGGGCCGGC ATGCCACCTT TCGGAACTTT GGCATGGCCT TCCTGACCCT CTTCCGAGTG 
TCCACAGGTG ACAACTGGAA CGGCATTATG AAGGACACCC TCCGGGACTG TGACCAGGAG 
TCCACATGCT ACAATACAGT CATCTCCCCC ATCTACTTCG TGTCCTTCGT GCTGACGGCC 
CAGTTTGTGC TGGTCAACGT GGTGATCGCT GTGCTGATGA AGCACCTGGA GGAAAGCAAC 
AAGGAGGCCA AAGAGGAGGC CGAGCTGGAG GCTGAGCTGG AGCTGGAGAT GAAGACGCTC 
AGCCCACAGC CCCACTCGCC ACTGGGCAGC CCCTTCCTCT GGCCGGGGGT CGAGGGCCCC 
GACAGCCCCG ATAGCCCCAA GCTCAGGGCC CCGCACACCG CGGCCCACAC GGGAGCAGCC 
TCGCGCTTCT CCCTGGAACA CCCCACGGAC AGACAGCTGT TTGACACCAT ATCCCTGCTG 
ATCCAGGGCT CCCTGGAAGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTCC CGCCCGCAGC CTGGGCGGCT CCGACCCACA GATGGAGCCC 
CACCCCGAGG AGATGCCCCT TGCACTGGGA CTGGACCTGC TGACTGTGAG GAAGTCTGGG 
GTCAGCCGGA CACACTCCCT GCCCAATGAC AGCTACATGT TCCGGGACGG AGGCACCGCT 
GAGGGGTCCC TAGGATGCAG AAGCTGGGGG CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA 
TCTGTCCACT CGCAGCCAGC AGACACCAGC TACATCCTGC AGCTTCCCAA AGACACACCT 
CACCTGCTCC AGCCCCACAG CGCTCCCACC TGGGGCACCA TTCCCAAACT GCCCCCACCA 
GGCCGCTCCC CCTTGGCTCA GAGGCCACTC AGGCGCCAGG CAGCAATAAG GACTGATTCT 
CTGGACGTTC AGGGCCTGGG CAGCCGGGAA GACCTGCTGT CCGAGGTGAG TGGGCCCTCC 
CCACCTCTGG CCCGGGCCTC CTCCTTCTGG GGCCACTTGG GCGCCCAGAT GCAGCAGCAT 
CCCCACCGCC AGAGCAAGAT CTCGAAGCAC ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA 
GAAACCACCT GGAGCAAAGA CCCACCCGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGACC GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA 
CGGGACCTGA AGAAGTGTCA CAGCGTGGAG GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC 
TGGCTGGATG AACAGAGGAG ACACTCCATC GCGGTCAGCT GCCTGGACGG CGGCTCCCAA 
CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC 
CGGCCCAAGA AAAAACTCAG CCCACCTAGT ATCTCCATAG ACCCCCCAGA GAGTCAGGGC 
CCTCGGCCCC CACCCAGCCC CGGCGTCTGC CTCCGGCGGA GGGCTCCGTC CAGCGACTCC 
AGGGACCCCT CGGCCTCCGG CCCCCCTGAC AGCATGGCTG CCTCGCCCCC CCCAAAGAAA 
GACGTGCTGA GTCTCTCTGG TTTAGCCTCT GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624712.1
CDS	504..7457
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624655.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624655.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAAGGCC CAGTGTAAAC CCTACTACTC AGACTACTCC 
CGATTCCGGC TCCTCGTCCA CCACTTGTGT ACCAGCCACT ATCTGGACCT CTTCATCACG 
GGTGTCATTG GTCTGAATGT GGTCACCATG GCCATGGAGC ACTACCAGCA GCCCCAGATC 
CTGGACGAGG CCTTGAAGAT CTGCAACTAT ATCTTCACCG TCATCTTTGT CTTGGAGTCA 
GTTTTTAAAC TCGTGGCCTT TGGCTTCCGT CGGTTCTTCC AGGACAGGTG GAACCAGCTG 
GACCTGGCCA TCGTGCTGCT GTCCATCATG GGCATCACGC TGGAGGAGAT TGAGGTCAAT 
GCCTCGCTGC CCATCAACCC CACCATCATC CGCATCATGA GAGTGCTGCG CATTGCCCGA 
GTGCTGAAGC TGCTGAAGAT GGCTGTGGGC ATGCGGGCGC TGCTGGACAC AGTCATGCAG 
GCCCTGCCTC AGGTGGGGAA CCTGGGACTT CTCTTCATGT TGTTGTTTTT CATCTTTGCA 
GCTCTGGGTG TGGAGCTCTT TGGAGACCTG GAGTGTGATG AGACGCACCC CTGCGAGGGC 
CTGGGCCGGC ATGCCACCTT TCGGAACTTT GGCATGGCCT TCCTGACCCT CTTCCGAGTG 
TCCACAGGTG ACAACTGGAA CGGCATTATG AAGGACACCC TCCGGGACTG TGACCAGGAG 
TCCACATGCT ACAATACAGT CATCTCCCCC ATCTACTTCG TGTCCTTCGT GCTGACGGCC 
CAGTTTGTGC TGGTCAACGT GGTGATCGCT GTGCTGATGA AGCACCTGGA GGAAAGCAAC 
AAGGAGGCCA AAGAGGAGGC CGAGCTGGAG GCTGAGCTGG AGCTGGAGAT GAAGACGCTC 
AGCCCACAGC CCCACTCGCC ACTGGGCAGC CCCTTCCTCT GGCCGGGGGT CGAGGGCCCC 
GACAGCCCCG ATAGCCCCAA GCTCAGGGCC CCGCACACCG CGGCCCACAC GGGAGCAGCC 
TCGCGCTTCT CCCTGGAACA CCCCACGGAC AGACAGCTGT TTGACACCAT ATCCCTGCTG 
ATCCAGGGCT CCCTGGAAGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTCC CGCCCGCAGC CTGGGCGGCT CCGACCCACA GATGGAGCCC 
CACCCCGAGG AGATGCCCCT TGCACTGGGA CTGGACCTGC TGACTGTGAG GAAGTCTGGG 
GTCAGCCGGA CACACTCCCT GCCCAATGAC AGCTACATGT TCCGGGACGG AGGCACCGCT 
GAGGGGTCCC TAGGATGCAG AAGCTGGGGG CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA 
TCTGTCCACT CGCAGCCAGC AGACACCAGC TACATCCTGC AGCTTCCCAA AGACACACCT 
CACCTGCTCC AGCCCCACAG CGCTCCCACC TGGGGCACCA TTCCCAAACT GCCCCCACCA 
GGCCGCTCCC CCTTGGCTCA GAGGCCACTC AGGCGCCAGG CAGCAATAAG GACTGATTCT 
CTGGACGTTC AGGGCCTGGG CAGCCGGGAA GACCTGCTGT CCGAGGTGAG TGGGCCCTCC 
CCACCTCTGG CCCGGGCCTC CTCCTTCTGG GGCCACTTGG GCGCCCAGAT GCAGCAGCAT 
CCCCACCGCC AGAGCAAGAT CTCGAAGCAC ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA 
GAAACCACCT GGAGCAAAGA CCCACCCGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGACC GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA 
CGGGACCTGA AGAAGTGTCA CAGCGTGGAG GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC 
TGGCTGGATG AACAGAGGAG ACACTCCATC GCGGTCAGCT GCCTGGACGG CGGCTCCCAA 
CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC 
CGGCCCAAGA AAAAACTCAG CCCACCTAGT ATCTCCATAG ACCCCCCAGA GAGTCAGGGC 
CCTCGGCCCC CACCCAGCCC CGGCGTCTGC CTCCGGCGGA GGGCTCCGTC CAGCGACTCC 
AGGGACCCCT CGGCCTCCGG CCCCCCTGAC AGCATGGCTG CCTCGCCCCC CCCAAAGAAA 
GACGTGCTGA GTCTCTCTGG TTTAGCCTCT GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624655.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624712.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X18, mRNA.

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CloneID	OCe11922
Clone ID Related Accession (Same CDS sequence)	XM_005624655.3 , XM_005624655.2
Accession Version	XM_005624655.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X18
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAAGGCC CAGTGTAAAC CCTACTACTC AGACTACTCC 
CGATTCCGGC TCCTCGTCCA CCACTTGTGT ACCAGCCACT ATCTGGACCT CTTCATCACG 
GGTGTCATTG GTCTGAATGT GGTCACCATG GCCATGGAGC ACTACCAGCA GCCCCAGATC 
CTGGACGAGG CCTTGAAGAT CTGCAACTAT ATCTTCACCG TCATCTTTGT CTTGGAGTCA 
GTTTTTAAAC TCGTGGCCTT TGGCTTCCGT CGGTTCTTCC AGGACAGGTG GAACCAGCTG 
GACCTGGCCA TCGTGCTGCT GTCCATCATG GGCATCACGC TGGAGGAGAT TGAGGTCAAT 
GCCTCGCTGC CCATCAACCC CACCATCATC CGCATCATGA GAGTGCTGCG CATTGCCCGA 
GTGCTGAAGC TGCTGAAGAT GGCTGTGGGC ATGCGGGCGC TGCTGGACAC AGTCATGCAG 
GCCCTGCCTC AGGTGGGGAA CCTGGGACTT CTCTTCATGT TGTTGTTTTT CATCTTTGCA 
GCTCTGGGTG TGGAGCTCTT TGGAGACCTG GAGTGTGATG AGACGCACCC CTGCGAGGGC 
CTGGGCCGGC ATGCCACCTT TCGGAACTTT GGCATGGCCT TCCTGACCCT CTTCCGAGTG 
TCCACAGGTG ACAACTGGAA CGGCATTATG AAGGACACCC TCCGGGACTG TGACCAGGAG 
TCCACATGCT ACAATACAGT CATCTCCCCC ATCTACTTCG TGTCCTTCGT GCTGACGGCC 
CAGTTTGTGC TGGTCAACGT GGTGATCGCT GTGCTGATGA AGCACCTGGA GGAAAGCAAC 
AAGGAGGCCA AAGAGGAGGC CGAGCTGGAG GCTGAGCTGG AGCTGGAGAT GAAGACGCTC 
AGCCCACAGC CCCACTCGCC ACTGGGCAGC CCCTTCCTCT GGCCGGGGGT CGAGGGCCCC 
GACAGCCCCG ATAGCCCCAA GCTCAGGGCC CCGCACACCG CGGCCCACAC GGGAGCAGCC 
TCGCGCTTCT CCCTGGAACA CCCCACGGAC AGACAGCTGT TTGACACCAT ATCCCTGCTG 
ATCCAGGGCT CCCTGGAAGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTCC CGCCCGCAGC CTGGGCGGCT CCGACCCACA GATGGAGCCC 
CACCCCGAGG AGATGCCCCT TGCACTGGGA CTGGACCTGC TGACTGTGAG GAAGTCTGGG 
GTCAGCCGGA CACACTCCCT GCCCAATGAC AGCTACATGT TCCGGGACGG AGGCACCGCT 
GAGGGGTCCC TAGGATGCAG AAGCTGGGGG CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA 
TCTGTCCACT CGCAGCCAGC AGACACCAGC TACATCCTGC AGCTTCCCAA AGACACACCT 
CACCTGCTCC AGCCCCACAG CGCTCCCACC TGGGGCACCA TTCCCAAACT GCCCCCACCA 
GGCCGCTCCC CCTTGGCTCA GAGGCCACTC AGGCGCCAGG CAGCAATAAG GACTGATTCT 
CTGGACGTTC AGGGCCTGGG CAGCCGGGAA GACCTGCTGT CCGAGGTGAG TGGGCCCTCC 
CCACCTCTGG CCCGGGCCTC CTCCTTCTGG GGCCACTTGG GCGCCCAGAT GCAGCAGCAT 
CCCCACCGCC AGAGCAAGAT CTCGAAGCAC ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA 
GAAACCACCT GGAGCAAAGA CCCACCCGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGACC GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA 
CGGGACCTGA AGAAGTGTCA CAGCGTGGAG GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC 
TGGCTGGATG AACAGAGGAG ACACTCCATC GCGGTCAGCT GCCTGGACGG CGGCTCCCAA 
CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC 
CGGCCCAAGA AAAAACTCAG CCCACCTAGT ATCTCCATAG ACCCCCCAGA GAGTCAGGGC 
CCTCGGCCCC CACCCAGCCC CGGCGTCTGC CTCCGGCGGA GGGCTCCGTC CAGCGACTCC 
AGGGACCCCT CGGCCTCCGG CCCCCCTGAC AGCATGGCTG CCTCGCCCCC CCCAAAGAAA 
GACGTGCTGA GTCTCTCTGG TTTAGCCTCT GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624712.1
CDS	527..7480
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624655.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624655.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAAGGCC CAGTGTAAAC CCTACTACTC AGACTACTCC 
CGATTCCGGC TCCTCGTCCA CCACTTGTGT ACCAGCCACT ATCTGGACCT CTTCATCACG 
GGTGTCATTG GTCTGAATGT GGTCACCATG GCCATGGAGC ACTACCAGCA GCCCCAGATC 
CTGGACGAGG CCTTGAAGAT CTGCAACTAT ATCTTCACCG TCATCTTTGT CTTGGAGTCA 
GTTTTTAAAC TCGTGGCCTT TGGCTTCCGT CGGTTCTTCC AGGACAGGTG GAACCAGCTG 
GACCTGGCCA TCGTGCTGCT GTCCATCATG GGCATCACGC TGGAGGAGAT TGAGGTCAAT 
GCCTCGCTGC CCATCAACCC CACCATCATC CGCATCATGA GAGTGCTGCG CATTGCCCGA 
GTGCTGAAGC TGCTGAAGAT GGCTGTGGGC ATGCGGGCGC TGCTGGACAC AGTCATGCAG 
GCCCTGCCTC AGGTGGGGAA CCTGGGACTT CTCTTCATGT TGTTGTTTTT CATCTTTGCA 
GCTCTGGGTG TGGAGCTCTT TGGAGACCTG GAGTGTGATG AGACGCACCC CTGCGAGGGC 
CTGGGCCGGC ATGCCACCTT TCGGAACTTT GGCATGGCCT TCCTGACCCT CTTCCGAGTG 
TCCACAGGTG ACAACTGGAA CGGCATTATG AAGGACACCC TCCGGGACTG TGACCAGGAG 
TCCACATGCT ACAATACAGT CATCTCCCCC ATCTACTTCG TGTCCTTCGT GCTGACGGCC 
CAGTTTGTGC TGGTCAACGT GGTGATCGCT GTGCTGATGA AGCACCTGGA GGAAAGCAAC 
AAGGAGGCCA AAGAGGAGGC CGAGCTGGAG GCTGAGCTGG AGCTGGAGAT GAAGACGCTC 
AGCCCACAGC CCCACTCGCC ACTGGGCAGC CCCTTCCTCT GGCCGGGGGT CGAGGGCCCC 
GACAGCCCCG ATAGCCCCAA GCTCAGGGCC CCGCACACCG CGGCCCACAC GGGAGCAGCC 
TCGCGCTTCT CCCTGGAACA CCCCACGGAC AGACAGCTGT TTGACACCAT ATCCCTGCTG 
ATCCAGGGCT CCCTGGAAGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTCC CGCCCGCAGC CTGGGCGGCT CCGACCCACA GATGGAGCCC 
CACCCCGAGG AGATGCCCCT TGCACTGGGA CTGGACCTGC TGACTGTGAG GAAGTCTGGG 
GTCAGCCGGA CACACTCCCT GCCCAATGAC AGCTACATGT TCCGGGACGG AGGCACCGCT 
GAGGGGTCCC TAGGATGCAG AAGCTGGGGG CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA 
TCTGTCCACT CGCAGCCAGC AGACACCAGC TACATCCTGC AGCTTCCCAA AGACACACCT 
CACCTGCTCC AGCCCCACAG CGCTCCCACC TGGGGCACCA TTCCCAAACT GCCCCCACCA 
GGCCGCTCCC CCTTGGCTCA GAGGCCACTC AGGCGCCAGG CAGCAATAAG GACTGATTCT 
CTGGACGTTC AGGGCCTGGG CAGCCGGGAA GACCTGCTGT CCGAGGTGAG TGGGCCCTCC 
CCACCTCTGG CCCGGGCCTC CTCCTTCTGG GGCCACTTGG GCGCCCAGAT GCAGCAGCAT 
CCCCACCGCC AGAGCAAGAT CTCGAAGCAC ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA 
GAAACCACCT GGAGCAAAGA CCCACCCGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGACC GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA 
CGGGACCTGA AGAAGTGTCA CAGCGTGGAG GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC 
TGGCTGGATG AACAGAGGAG ACACTCCATC GCGGTCAGCT GCCTGGACGG CGGCTCCCAA 
CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC 
CGGCCCAAGA AAAAACTCAG CCCACCTAGT ATCTCCATAG ACCCCCCAGA GAGTCAGGGC 
CCTCGGCCCC CACCCAGCCC CGGCGTCTGC CTCCGGCGGA GGGCTCCGTC CAGCGACTCC 
AGGGACCCCT CGGCCTCCGG CCCCCCTGAC AGCATGGCTG CCTCGCCCCC CCCAAAGAAA 
GACGTGCTGA GTCTCTCTGG TTTAGCCTCT GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860703.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865796.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X16, mRNA.

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CloneID	OCe11923
Clone ID Related Accession (Same CDS sequence)	XM_860703.5 , XM_860703.4
Accession Version	XM_860703.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_860703.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAATCT AATGCTGGAC GATGTAATTG CTTCCGGCAG CTCATCCAGC 
GCGGCGCCAG AGGCCCAGTG TAAACCCTAC TACTCAGACT ACTCCCGATT CCGGCTCCTC 
GTCCACCACT TGTGTACCAG CCACTATCTG GACCTCTTCA TCACGGGTGT CATTGGTCTG 
AATGTGGTCA CCATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA CGAGGCCTTG 
AAGATCTGCA ACTATATCTT CACCGTCATC TTTGTCTTGG AGTCAGTTTT TAAACTCGTG 
GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATCGTG 
CTGCTGTCCA TCATGGGCAT CACGCTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGAGTG CTGCGCATTG CCCGAGTGCT GAAGCTGCTG 
AAGATGGCTG TGGGCATGCG GGCGCTGCTG GACACAGTCA TGCAGGCCCT GCCTCAGGTG 
GGGAACCTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGTGTGGAG 
CTCTTTGGAG ACCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC 
ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG ACCCTCTTCC GAGTGTCCAC AGGTGACAAC 
TGGAACGGCA TTATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC ATGCTACAAT 
ACAGTCATCT CCCCCATCTA CTTCGTGTCC TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC 
AACGTGGTGA TCGCTGTGCT GATGAAGCAC CTGGAGGAAA GCAACAAGGA GGCCAAAGAG 
GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG GAGATGAAGA CGCTCAGCCC ACAGCCCCAC 
TCGCCACTGG GCAGCCCCTT CCTCTGGCCG GGGGTCGAGG GCCCCGACAG CCCCGATAGC 
CCCAAGCTCA GGGCCCCGCA CACCGCGGCC CACACGGGAG CAGCCTCGCG CTTCTCCCTG 
GAACACCCCA CGATCCCTCT AGCTGAGATG GAGGCTCTGT CTCTGACGTC AGAGATTGTG 
TCTGAACCGT CCCACTCTCT AGCTCTGACG GATGACTCTT TGCCTGATGA CACTCACACA 
CTCTTACCTA GTGCCCTGGA CAGCAATATG GAGCCCCACC CCGAGGAGAT GCCCCTTGCA 
CTGGGACTGG ACCTGCTGAC TGTGAGGAAG TCTGGGGTCA GCCGGACACA CTCCCTGCCC 
AATGACAGCT ACATGTTCCG GGACGGAGGC ACCGCTGAGG GGTCCCTAGG ATGCAGAAGC 
TGGGGGCTCC CCAAAGCTCA GTCAGGCTCT GTCTTATCTG TCCACTCGCA GCCAGCAGAC 
ACCAGCTACA TCCTGCAGCT TCCCAAAGAC ACACCTCACC TGCTCCAGCC CCACAGCGCT 
CCCACCTGGG GCACCATTCC CAAACTGCCC CCACCAGGCC GCTCCCCCTT GGCTCAGAGG 
CCACTCAGGC GCCAGGCAGC AATAAGGACT GATTCTCTGG ACGTTCAGGG CCTGGGCAGC 
CGGGAAGACC TGCTGTCCGA GGTGAGTGGG CCCTCCCCAC CTCTGGCCCG GGCCTCCTCC 
TTCTGGGGCC ACTTGGGCGC CCAGATGCAG CAGCATCCCC ACCGCCAGAG CAAGATCTCG 
AAGCACATGC CCCCGCCAGC CCCCCGCCCA GGCTCAGAAA CCACCTGGAG CAAAGACCCA 
CCCGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGACCGTGG GCAGCCAGGA GGAGCCCCCG TCCCCACGGG ACCTGAAGAA GTGTCACAGC 
GTGGAGGCCC AGAGCTGCCG GCGCAGGCCT GCGTCCTGGC TGGATGAACA GAGGAGACAC 
TCCATCGCGG TCAGCTGCCT GGACGGCGGC TCCCAACCCA GCCTGGGCCC CGGCCCCTCT 
AGCCTTGGCG GCCAGCCTCT TGGGGGACCT GGAAGCCGGC CCAAGAAAAA ACTCAGCCCA 
CCTAGTATCT CCATAGACCC CCCAGAGAGT CAGGGCCCTC GGCCCCCACC CAGCCCCGGC 
GTCTGCCTCC GGCGGAGGGC TCCGTCCAGC GACTCCAGGG ACCCCTCGGC CTCCGGCCCC 
CCTGACAGCA TGGCTGCCTC GCCCCCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
GCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865796.1
CDS	504..7433
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860703.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860703.5

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAATCT AATGCTGGAC GATGTAATTG CTTCCGGCAG CTCATCCAGC 
GCGGCGCCAG AGGCCCAGTG TAAACCCTAC TACTCAGACT ACTCCCGATT CCGGCTCCTC 
GTCCACCACT TGTGTACCAG CCACTATCTG GACCTCTTCA TCACGGGTGT CATTGGTCTG 
AATGTGGTCA CCATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA CGAGGCCTTG 
AAGATCTGCA ACTATATCTT CACCGTCATC TTTGTCTTGG AGTCAGTTTT TAAACTCGTG 
GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATCGTG 
CTGCTGTCCA TCATGGGCAT CACGCTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGAGTG CTGCGCATTG CCCGAGTGCT GAAGCTGCTG 
AAGATGGCTG TGGGCATGCG GGCGCTGCTG GACACAGTCA TGCAGGCCCT GCCTCAGGTG 
GGGAACCTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGTGTGGAG 
CTCTTTGGAG ACCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC 
ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG ACCCTCTTCC GAGTGTCCAC AGGTGACAAC 
TGGAACGGCA TTATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC ATGCTACAAT 
ACAGTCATCT CCCCCATCTA CTTCGTGTCC TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC 
AACGTGGTGA TCGCTGTGCT GATGAAGCAC CTGGAGGAAA GCAACAAGGA GGCCAAAGAG 
GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG GAGATGAAGA CGCTCAGCCC ACAGCCCCAC 
TCGCCACTGG GCAGCCCCTT CCTCTGGCCG GGGGTCGAGG GCCCCGACAG CCCCGATAGC 
CCCAAGCTCA GGGCCCCGCA CACCGCGGCC CACACGGGAG CAGCCTCGCG CTTCTCCCTG 
GAACACCCCA CGATCCCTCT AGCTGAGATG GAGGCTCTGT CTCTGACGTC AGAGATTGTG 
TCTGAACCGT CCCACTCTCT AGCTCTGACG GATGACTCTT TGCCTGATGA CACTCACACA 
CTCTTACCTA GTGCCCTGGA CAGCAATATG GAGCCCCACC CCGAGGAGAT GCCCCTTGCA 
CTGGGACTGG ACCTGCTGAC TGTGAGGAAG TCTGGGGTCA GCCGGACACA CTCCCTGCCC 
AATGACAGCT ACATGTTCCG GGACGGAGGC ACCGCTGAGG GGTCCCTAGG ATGCAGAAGC 
TGGGGGCTCC CCAAAGCTCA GTCAGGCTCT GTCTTATCTG TCCACTCGCA GCCAGCAGAC 
ACCAGCTACA TCCTGCAGCT TCCCAAAGAC ACACCTCACC TGCTCCAGCC CCACAGCGCT 
CCCACCTGGG GCACCATTCC CAAACTGCCC CCACCAGGCC GCTCCCCCTT GGCTCAGAGG 
CCACTCAGGC GCCAGGCAGC AATAAGGACT GATTCTCTGG ACGTTCAGGG CCTGGGCAGC 
CGGGAAGACC TGCTGTCCGA GGTGAGTGGG CCCTCCCCAC CTCTGGCCCG GGCCTCCTCC 
TTCTGGGGCC ACTTGGGCGC CCAGATGCAG CAGCATCCCC ACCGCCAGAG CAAGATCTCG 
AAGCACATGC CCCCGCCAGC CCCCCGCCCA GGCTCAGAAA CCACCTGGAG CAAAGACCCA 
CCCGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGACCGTGG GCAGCCAGGA GGAGCCCCCG TCCCCACGGG ACCTGAAGAA GTGTCACAGC 
GTGGAGGCCC AGAGCTGCCG GCGCAGGCCT GCGTCCTGGC TGGATGAACA GAGGAGACAC 
TCCATCGCGG TCAGCTGCCT GGACGGCGGC TCCCAACCCA GCCTGGGCCC CGGCCCCTCT 
AGCCTTGGCG GCCAGCCTCT TGGGGGACCT GGAAGCCGGC CCAAGAAAAA ACTCAGCCCA 
CCTAGTATCT CCATAGACCC CCCAGAGAGT CAGGGCCCTC GGCCCCCACC CAGCCCCGGC 
GTCTGCCTCC GGCGGAGGGC TCCGTCCAGC GACTCCAGGG ACCCCTCGGC CTCCGGCCCC 
CCTGACAGCA TGGCTGCCTC GCCCCCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
GCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860703.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865796.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X19, mRNA.

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CloneID	OCe11923
Clone ID Related Accession (Same CDS sequence)	XM_860703.5 , XM_860703.4
Accession Version	XM_860703.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X19
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_860703.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAATCT AATGCTGGAC GATGTAATTG CTTCCGGCAG CTCATCCAGC 
GCGGCGCCAG AGGCCCAGTG TAAACCCTAC TACTCAGACT ACTCCCGATT CCGGCTCCTC 
GTCCACCACT TGTGTACCAG CCACTATCTG GACCTCTTCA TCACGGGTGT CATTGGTCTG 
AATGTGGTCA CCATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA CGAGGCCTTG 
AAGATCTGCA ACTATATCTT CACCGTCATC TTTGTCTTGG AGTCAGTTTT TAAACTCGTG 
GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATCGTG 
CTGCTGTCCA TCATGGGCAT CACGCTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGAGTG CTGCGCATTG CCCGAGTGCT GAAGCTGCTG 
AAGATGGCTG TGGGCATGCG GGCGCTGCTG GACACAGTCA TGCAGGCCCT GCCTCAGGTG 
GGGAACCTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGTGTGGAG 
CTCTTTGGAG ACCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC 
ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG ACCCTCTTCC GAGTGTCCAC AGGTGACAAC 
TGGAACGGCA TTATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC ATGCTACAAT 
ACAGTCATCT CCCCCATCTA CTTCGTGTCC TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC 
AACGTGGTGA TCGCTGTGCT GATGAAGCAC CTGGAGGAAA GCAACAAGGA GGCCAAAGAG 
GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG GAGATGAAGA CGCTCAGCCC ACAGCCCCAC 
TCGCCACTGG GCAGCCCCTT CCTCTGGCCG GGGGTCGAGG GCCCCGACAG CCCCGATAGC 
CCCAAGCTCA GGGCCCCGCA CACCGCGGCC CACACGGGAG CAGCCTCGCG CTTCTCCCTG 
GAACACCCCA CGATCCCTCT AGCTGAGATG GAGGCTCTGT CTCTGACGTC AGAGATTGTG 
TCTGAACCGT CCCACTCTCT AGCTCTGACG GATGACTCTT TGCCTGATGA CACTCACACA 
CTCTTACCTA GTGCCCTGGA CAGCAATATG GAGCCCCACC CCGAGGAGAT GCCCCTTGCA 
CTGGGACTGG ACCTGCTGAC TGTGAGGAAG TCTGGGGTCA GCCGGACACA CTCCCTGCCC 
AATGACAGCT ACATGTTCCG GGACGGAGGC ACCGCTGAGG GGTCCCTAGG ATGCAGAAGC 
TGGGGGCTCC CCAAAGCTCA GTCAGGCTCT GTCTTATCTG TCCACTCGCA GCCAGCAGAC 
ACCAGCTACA TCCTGCAGCT TCCCAAAGAC ACACCTCACC TGCTCCAGCC CCACAGCGCT 
CCCACCTGGG GCACCATTCC CAAACTGCCC CCACCAGGCC GCTCCCCCTT GGCTCAGAGG 
CCACTCAGGC GCCAGGCAGC AATAAGGACT GATTCTCTGG ACGTTCAGGG CCTGGGCAGC 
CGGGAAGACC TGCTGTCCGA GGTGAGTGGG CCCTCCCCAC CTCTGGCCCG GGCCTCCTCC 
TTCTGGGGCC ACTTGGGCGC CCAGATGCAG CAGCATCCCC ACCGCCAGAG CAAGATCTCG 
AAGCACATGC CCCCGCCAGC CCCCCGCCCA GGCTCAGAAA CCACCTGGAG CAAAGACCCA 
CCCGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGACCGTGG GCAGCCAGGA GGAGCCCCCG TCCCCACGGG ACCTGAAGAA GTGTCACAGC 
GTGGAGGCCC AGAGCTGCCG GCGCAGGCCT GCGTCCTGGC TGGATGAACA GAGGAGACAC 
TCCATCGCGG TCAGCTGCCT GGACGGCGGC TCCCAACCCA GCCTGGGCCC CGGCCCCTCT 
AGCCTTGGCG GCCAGCCTCT TGGGGGACCT GGAAGCCGGC CCAAGAAAAA ACTCAGCCCA 
CCTAGTATCT CCATAGACCC CCCAGAGAGT CAGGGCCCTC GGCCCCCACC CAGCCCCGGC 
GTCTGCCTCC GGCGGAGGGC TCCGTCCAGC GACTCCAGGG ACCCCTCGGC CTCCGGCCCC 
CCTGACAGCA TGGCTGCCTC GCCCCCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
GCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865796.1
CDS	527..7456
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860703.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860703.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAATCT AATGCTGGAC GATGTAATTG CTTCCGGCAG CTCATCCAGC 
GCGGCGCCAG AGGCCCAGTG TAAACCCTAC TACTCAGACT ACTCCCGATT CCGGCTCCTC 
GTCCACCACT TGTGTACCAG CCACTATCTG GACCTCTTCA TCACGGGTGT CATTGGTCTG 
AATGTGGTCA CCATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA CGAGGCCTTG 
AAGATCTGCA ACTATATCTT CACCGTCATC TTTGTCTTGG AGTCAGTTTT TAAACTCGTG 
GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATCGTG 
CTGCTGTCCA TCATGGGCAT CACGCTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGAGTG CTGCGCATTG CCCGAGTGCT GAAGCTGCTG 
AAGATGGCTG TGGGCATGCG GGCGCTGCTG GACACAGTCA TGCAGGCCCT GCCTCAGGTG 
GGGAACCTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGTGTGGAG 
CTCTTTGGAG ACCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC 
ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG ACCCTCTTCC GAGTGTCCAC AGGTGACAAC 
TGGAACGGCA TTATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC ATGCTACAAT 
ACAGTCATCT CCCCCATCTA CTTCGTGTCC TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC 
AACGTGGTGA TCGCTGTGCT GATGAAGCAC CTGGAGGAAA GCAACAAGGA GGCCAAAGAG 
GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG GAGATGAAGA CGCTCAGCCC ACAGCCCCAC 
TCGCCACTGG GCAGCCCCTT CCTCTGGCCG GGGGTCGAGG GCCCCGACAG CCCCGATAGC 
CCCAAGCTCA GGGCCCCGCA CACCGCGGCC CACACGGGAG CAGCCTCGCG CTTCTCCCTG 
GAACACCCCA CGATCCCTCT AGCTGAGATG GAGGCTCTGT CTCTGACGTC AGAGATTGTG 
TCTGAACCGT CCCACTCTCT AGCTCTGACG GATGACTCTT TGCCTGATGA CACTCACACA 
CTCTTACCTA GTGCCCTGGA CAGCAATATG GAGCCCCACC CCGAGGAGAT GCCCCTTGCA 
CTGGGACTGG ACCTGCTGAC TGTGAGGAAG TCTGGGGTCA GCCGGACACA CTCCCTGCCC 
AATGACAGCT ACATGTTCCG GGACGGAGGC ACCGCTGAGG GGTCCCTAGG ATGCAGAAGC 
TGGGGGCTCC CCAAAGCTCA GTCAGGCTCT GTCTTATCTG TCCACTCGCA GCCAGCAGAC 
ACCAGCTACA TCCTGCAGCT TCCCAAAGAC ACACCTCACC TGCTCCAGCC CCACAGCGCT 
CCCACCTGGG GCACCATTCC CAAACTGCCC CCACCAGGCC GCTCCCCCTT GGCTCAGAGG 
CCACTCAGGC GCCAGGCAGC AATAAGGACT GATTCTCTGG ACGTTCAGGG CCTGGGCAGC 
CGGGAAGACC TGCTGTCCGA GGTGAGTGGG CCCTCCCCAC CTCTGGCCCG GGCCTCCTCC 
TTCTGGGGCC ACTTGGGCGC CCAGATGCAG CAGCATCCCC ACCGCCAGAG CAAGATCTCG 
AAGCACATGC CCCCGCCAGC CCCCCGCCCA GGCTCAGAAA CCACCTGGAG CAAAGACCCA 
CCCGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGACCGTGG GCAGCCAGGA GGAGCCCCCG TCCCCACGGG ACCTGAAGAA GTGTCACAGC 
GTGGAGGCCC AGAGCTGCCG GCGCAGGCCT GCGTCCTGGC TGGATGAACA GAGGAGACAC 
TCCATCGCGG TCAGCTGCCT GGACGGCGGC TCCCAACCCA GCCTGGGCCC CGGCCCCTCT 
AGCCTTGGCG GCCAGCCTCT TGGGGGACCT GGAAGCCGGC CCAAGAAAAA ACTCAGCCCA 
CCTAGTATCT CCATAGACCC CCCAGAGAGT CAGGGCCCTC GGCCCCCACC CAGCCCCGGC 
GTCTGCCTCC GGCGGAGGGC TCCGTCCAGC GACTCCAGGG ACCCCTCGGC CTCCGGCCCC 
CCTGACAGCA TGGCTGCCTC GCCCCCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
GCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860688.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865781.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X17, mRNA.

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CloneID	OCe11924
Clone ID Related Accession (Same CDS sequence)	XM_860688.5 , XM_860688.4
Accession Version	XM_860688.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_860688.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGAGG CCCAGTGTAA ACCCTACTAC 
TCAGACTACT CCCGATTCCG GCTCCTCGTC CACCACTTGT GTACCAGCCA CTATCTGGAC 
CTCTTCATCA CGGGTGTCAT TGGTCTGAAT GTGGTCACCA TGGCCATGGA GCACTACCAG 
CAGCCCCAGA TCCTGGACGA GGCCTTGAAG ATCTGCAACT ATATCTTCAC CGTCATCTTT 
GTCTTGGAGT CAGTTTTTAA ACTCGTGGCC TTTGGCTTCC GTCGGTTCTT CCAGGACAGG 
TGGAACCAGC TGGACCTGGC CATCGTGCTG CTGTCCATCA TGGGCATCAC GCTGGAGGAG 
ATTGAGGTCA ATGCCTCGCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGAGTGCTG 
CGCATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCTGTGG GCATGCGGGC GCTGCTGGAC 
ACAGTCATGC AGGCCCTGCC TCAGGTGGGG AACCTGGGAC TTCTCTTCAT GTTGTTGTTT 
TTCATCTTTG CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC 
CCCTGCGAGG GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGACC 
CTCTTCCGAG TGTCCACAGG TGACAACTGG AACGGCATTA TGAAGGACAC CCTCCGGGAC 
TGTGACCAGG AGTCCACATG CTACAATACA GTCATCTCCC CCATCTACTT CGTGTCCTTC 
GTGCTGACGG CCCAGTTTGT GCTGGTCAAC GTGGTGATCG CTGTGCTGAT GAAGCACCTG 
GAGGAAAGCA ACAAGGAGGC CAAAGAGGAG GCCGAGCTGG AGGCTGAGCT GGAGCTGGAG 
ATGAAGACGC TCAGCCCACA GCCCCACTCG CCACTGGGCA GCCCCTTCCT CTGGCCGGGG 
GTCGAGGGCC CCGACAGCCC CGATAGCCCC AAGCTCAGGG CCCCGCACAC CGCGGCCCAC 
ACGGGAGCAG CCTCGCGCTT CTCCCTGGAA CACCCCACGG ACAGACAGCT GTTTGACACC 
ATATCCCTGC TGATCCAGGG CTCCCTGGAA GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT CCCGCCCGCA GCCTGGGCGG CTCCGACCCA 
CAGATGGAGC CCCACCCCGA GGAGATGCCC CTTGCACTGG GACTGGACCT GCTGACTGTG 
AGGAAGTCTG GGGTCAGCCG GACACACTCC CTGCCCAATG ACAGCTACAT GTTCCGGGAC 
GGAGGCACCG CTGAGGGGTC CCTAGGATGC AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA 
GGCTCTGTCT TATCTGTCCA CTCGCAGCCA GCAGACACCA GCTACATCCT GCAGCTTCCC 
AAAGACACAC CTCACCTGCT CCAGCCCCAC AGCGCTCCCA CCTGGGGCAC CATTCCCAAA 
CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT CAGAGGCCAC TCAGGCGCCA GGCAGCAATA 
AGGACTGATT CTCTGGACGT TCAGGGCCTG GGCAGCCGGG AAGACCTGCT GTCCGAGGTG 
AGTGGGCCCT CCCCACCTCT GGCCCGGGCC TCCTCCTTCT GGGGCCACTT GGGCGCCCAG 
ATGCAGCAGC ATCCCCACCG CCAGAGCAAG ATCTCGAAGC ACATGCCCCC GCCAGCCCCC 
CGCCCAGGCT CAGAAACCAC CTGGAGCAAA GACCCACCCG AGACCAGAAG CAGCTTAGAG 
CTGGACACGG AGCTGAGCTG GATTTCAGGA GACCTCCTGA CCGTGGGCAG CCAGGAGGAG 
CCCCCGTCCC CACGGGACCT GAAGAAGTGT CACAGCGTGG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGCGT CCTGGCTGGA TGAACAGAGG AGACACTCCA TCGCGGTCAG CTGCCTGGAC 
GGCGGCTCCC AACCCAGCCT GGGCCCCGGC CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG 
GGACCTGGAA GCCGGCCCAA GAAAAAACTC AGCCCACCTA GTATCTCCAT AGACCCCCCA 
GAGAGTCAGG GCCCTCGGCC CCCACCCAGC CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG 
TCCAGCGACT CCAGGGACCC CTCGGCCTCC GGCCCCCCTG ACAGCATGGC TGCCTCGCCC 
CCCCCAAAGA AAGACGTGCT GAGTCTCTCT GGTTTAGCCT CTGACCCAGC AGACCTGGAC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865781.1
CDS	504..7409
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860688.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860688.5

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGAGG CCCAGTGTAA ACCCTACTAC 
TCAGACTACT CCCGATTCCG GCTCCTCGTC CACCACTTGT GTACCAGCCA CTATCTGGAC 
CTCTTCATCA CGGGTGTCAT TGGTCTGAAT GTGGTCACCA TGGCCATGGA GCACTACCAG 
CAGCCCCAGA TCCTGGACGA GGCCTTGAAG ATCTGCAACT ATATCTTCAC CGTCATCTTT 
GTCTTGGAGT CAGTTTTTAA ACTCGTGGCC TTTGGCTTCC GTCGGTTCTT CCAGGACAGG 
TGGAACCAGC TGGACCTGGC CATCGTGCTG CTGTCCATCA TGGGCATCAC GCTGGAGGAG 
ATTGAGGTCA ATGCCTCGCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGAGTGCTG 
CGCATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCTGTGG GCATGCGGGC GCTGCTGGAC 
ACAGTCATGC AGGCCCTGCC TCAGGTGGGG AACCTGGGAC TTCTCTTCAT GTTGTTGTTT 
TTCATCTTTG CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC 
CCCTGCGAGG GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGACC 
CTCTTCCGAG TGTCCACAGG TGACAACTGG AACGGCATTA TGAAGGACAC CCTCCGGGAC 
TGTGACCAGG AGTCCACATG CTACAATACA GTCATCTCCC CCATCTACTT CGTGTCCTTC 
GTGCTGACGG CCCAGTTTGT GCTGGTCAAC GTGGTGATCG CTGTGCTGAT GAAGCACCTG 
GAGGAAAGCA ACAAGGAGGC CAAAGAGGAG GCCGAGCTGG AGGCTGAGCT GGAGCTGGAG 
ATGAAGACGC TCAGCCCACA GCCCCACTCG CCACTGGGCA GCCCCTTCCT CTGGCCGGGG 
GTCGAGGGCC CCGACAGCCC CGATAGCCCC AAGCTCAGGG CCCCGCACAC CGCGGCCCAC 
ACGGGAGCAG CCTCGCGCTT CTCCCTGGAA CACCCCACGG ACAGACAGCT GTTTGACACC 
ATATCCCTGC TGATCCAGGG CTCCCTGGAA GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT CCCGCCCGCA GCCTGGGCGG CTCCGACCCA 
CAGATGGAGC CCCACCCCGA GGAGATGCCC CTTGCACTGG GACTGGACCT GCTGACTGTG 
AGGAAGTCTG GGGTCAGCCG GACACACTCC CTGCCCAATG ACAGCTACAT GTTCCGGGAC 
GGAGGCACCG CTGAGGGGTC CCTAGGATGC AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA 
GGCTCTGTCT TATCTGTCCA CTCGCAGCCA GCAGACACCA GCTACATCCT GCAGCTTCCC 
AAAGACACAC CTCACCTGCT CCAGCCCCAC AGCGCTCCCA CCTGGGGCAC CATTCCCAAA 
CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT CAGAGGCCAC TCAGGCGCCA GGCAGCAATA 
AGGACTGATT CTCTGGACGT TCAGGGCCTG GGCAGCCGGG AAGACCTGCT GTCCGAGGTG 
AGTGGGCCCT CCCCACCTCT GGCCCGGGCC TCCTCCTTCT GGGGCCACTT GGGCGCCCAG 
ATGCAGCAGC ATCCCCACCG CCAGAGCAAG ATCTCGAAGC ACATGCCCCC GCCAGCCCCC 
CGCCCAGGCT CAGAAACCAC CTGGAGCAAA GACCCACCCG AGACCAGAAG CAGCTTAGAG 
CTGGACACGG AGCTGAGCTG GATTTCAGGA GACCTCCTGA CCGTGGGCAG CCAGGAGGAG 
CCCCCGTCCC CACGGGACCT GAAGAAGTGT CACAGCGTGG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGCGT CCTGGCTGGA TGAACAGAGG AGACACTCCA TCGCGGTCAG CTGCCTGGAC 
GGCGGCTCCC AACCCAGCCT GGGCCCCGGC CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG 
GGACCTGGAA GCCGGCCCAA GAAAAAACTC AGCCCACCTA GTATCTCCAT AGACCCCCCA 
GAGAGTCAGG GCCCTCGGCC CCCACCCAGC CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG 
TCCAGCGACT CCAGGGACCC CTCGGCCTCC GGCCCCCCTG ACAGCATGGC TGCCTCGCCC 
CCCCCAAAGA AAGACGTGCT GAGTCTCTCT GGTTTAGCCT CTGACCCAGC AGACCTGGAC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860688.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865781.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X20, mRNA.

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CloneID	OCe11924
Clone ID Related Accession (Same CDS sequence)	XM_860688.5 , XM_860688.4
Accession Version	XM_860688.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X20
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_860688.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGAGG CCCAGTGTAA ACCCTACTAC 
TCAGACTACT CCCGATTCCG GCTCCTCGTC CACCACTTGT GTACCAGCCA CTATCTGGAC 
CTCTTCATCA CGGGTGTCAT TGGTCTGAAT GTGGTCACCA TGGCCATGGA GCACTACCAG 
CAGCCCCAGA TCCTGGACGA GGCCTTGAAG ATCTGCAACT ATATCTTCAC CGTCATCTTT 
GTCTTGGAGT CAGTTTTTAA ACTCGTGGCC TTTGGCTTCC GTCGGTTCTT CCAGGACAGG 
TGGAACCAGC TGGACCTGGC CATCGTGCTG CTGTCCATCA TGGGCATCAC GCTGGAGGAG 
ATTGAGGTCA ATGCCTCGCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGAGTGCTG 
CGCATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCTGTGG GCATGCGGGC GCTGCTGGAC 
ACAGTCATGC AGGCCCTGCC TCAGGTGGGG AACCTGGGAC TTCTCTTCAT GTTGTTGTTT 
TTCATCTTTG CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC 
CCCTGCGAGG GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGACC 
CTCTTCCGAG TGTCCACAGG TGACAACTGG AACGGCATTA TGAAGGACAC CCTCCGGGAC 
TGTGACCAGG AGTCCACATG CTACAATACA GTCATCTCCC CCATCTACTT CGTGTCCTTC 
GTGCTGACGG CCCAGTTTGT GCTGGTCAAC GTGGTGATCG CTGTGCTGAT GAAGCACCTG 
GAGGAAAGCA ACAAGGAGGC CAAAGAGGAG GCCGAGCTGG AGGCTGAGCT GGAGCTGGAG 
ATGAAGACGC TCAGCCCACA GCCCCACTCG CCACTGGGCA GCCCCTTCCT CTGGCCGGGG 
GTCGAGGGCC CCGACAGCCC CGATAGCCCC AAGCTCAGGG CCCCGCACAC CGCGGCCCAC 
ACGGGAGCAG CCTCGCGCTT CTCCCTGGAA CACCCCACGG ACAGACAGCT GTTTGACACC 
ATATCCCTGC TGATCCAGGG CTCCCTGGAA GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT CCCGCCCGCA GCCTGGGCGG CTCCGACCCA 
CAGATGGAGC CCCACCCCGA GGAGATGCCC CTTGCACTGG GACTGGACCT GCTGACTGTG 
AGGAAGTCTG GGGTCAGCCG GACACACTCC CTGCCCAATG ACAGCTACAT GTTCCGGGAC 
GGAGGCACCG CTGAGGGGTC CCTAGGATGC AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA 
GGCTCTGTCT TATCTGTCCA CTCGCAGCCA GCAGACACCA GCTACATCCT GCAGCTTCCC 
AAAGACACAC CTCACCTGCT CCAGCCCCAC AGCGCTCCCA CCTGGGGCAC CATTCCCAAA 
CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT CAGAGGCCAC TCAGGCGCCA GGCAGCAATA 
AGGACTGATT CTCTGGACGT TCAGGGCCTG GGCAGCCGGG AAGACCTGCT GTCCGAGGTG 
AGTGGGCCCT CCCCACCTCT GGCCCGGGCC TCCTCCTTCT GGGGCCACTT GGGCGCCCAG 
ATGCAGCAGC ATCCCCACCG CCAGAGCAAG ATCTCGAAGC ACATGCCCCC GCCAGCCCCC 
CGCCCAGGCT CAGAAACCAC CTGGAGCAAA GACCCACCCG AGACCAGAAG CAGCTTAGAG 
CTGGACACGG AGCTGAGCTG GATTTCAGGA GACCTCCTGA CCGTGGGCAG CCAGGAGGAG 
CCCCCGTCCC CACGGGACCT GAAGAAGTGT CACAGCGTGG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGCGT CCTGGCTGGA TGAACAGAGG AGACACTCCA TCGCGGTCAG CTGCCTGGAC 
GGCGGCTCCC AACCCAGCCT GGGCCCCGGC CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG 
GGACCTGGAA GCCGGCCCAA GAAAAAACTC AGCCCACCTA GTATCTCCAT AGACCCCCCA 
GAGAGTCAGG GCCCTCGGCC CCCACCCAGC CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG 
TCCAGCGACT CCAGGGACCC CTCGGCCTCC GGCCCCCCTG ACAGCATGGC TGCCTCGCCC 
CCCCCAAAGA AAGACGTGCT GAGTCTCTCT GGTTTAGCCT CTGACCCAGC AGACCTGGAC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865781.1
CDS	527..7432
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860688.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860688.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGAGG CCCAGTGTAA ACCCTACTAC 
TCAGACTACT CCCGATTCCG GCTCCTCGTC CACCACTTGT GTACCAGCCA CTATCTGGAC 
CTCTTCATCA CGGGTGTCAT TGGTCTGAAT GTGGTCACCA TGGCCATGGA GCACTACCAG 
CAGCCCCAGA TCCTGGACGA GGCCTTGAAG ATCTGCAACT ATATCTTCAC CGTCATCTTT 
GTCTTGGAGT CAGTTTTTAA ACTCGTGGCC TTTGGCTTCC GTCGGTTCTT CCAGGACAGG 
TGGAACCAGC TGGACCTGGC CATCGTGCTG CTGTCCATCA TGGGCATCAC GCTGGAGGAG 
ATTGAGGTCA ATGCCTCGCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGAGTGCTG 
CGCATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCTGTGG GCATGCGGGC GCTGCTGGAC 
ACAGTCATGC AGGCCCTGCC TCAGGTGGGG AACCTGGGAC TTCTCTTCAT GTTGTTGTTT 
TTCATCTTTG CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC 
CCCTGCGAGG GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGACC 
CTCTTCCGAG TGTCCACAGG TGACAACTGG AACGGCATTA TGAAGGACAC CCTCCGGGAC 
TGTGACCAGG AGTCCACATG CTACAATACA GTCATCTCCC CCATCTACTT CGTGTCCTTC 
GTGCTGACGG CCCAGTTTGT GCTGGTCAAC GTGGTGATCG CTGTGCTGAT GAAGCACCTG 
GAGGAAAGCA ACAAGGAGGC CAAAGAGGAG GCCGAGCTGG AGGCTGAGCT GGAGCTGGAG 
ATGAAGACGC TCAGCCCACA GCCCCACTCG CCACTGGGCA GCCCCTTCCT CTGGCCGGGG 
GTCGAGGGCC CCGACAGCCC CGATAGCCCC AAGCTCAGGG CCCCGCACAC CGCGGCCCAC 
ACGGGAGCAG CCTCGCGCTT CTCCCTGGAA CACCCCACGG ACAGACAGCT GTTTGACACC 
ATATCCCTGC TGATCCAGGG CTCCCTGGAA GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT CCCGCCCGCA GCCTGGGCGG CTCCGACCCA 
CAGATGGAGC CCCACCCCGA GGAGATGCCC CTTGCACTGG GACTGGACCT GCTGACTGTG 
AGGAAGTCTG GGGTCAGCCG GACACACTCC CTGCCCAATG ACAGCTACAT GTTCCGGGAC 
GGAGGCACCG CTGAGGGGTC CCTAGGATGC AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA 
GGCTCTGTCT TATCTGTCCA CTCGCAGCCA GCAGACACCA GCTACATCCT GCAGCTTCCC 
AAAGACACAC CTCACCTGCT CCAGCCCCAC AGCGCTCCCA CCTGGGGCAC CATTCCCAAA 
CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT CAGAGGCCAC TCAGGCGCCA GGCAGCAATA 
AGGACTGATT CTCTGGACGT TCAGGGCCTG GGCAGCCGGG AAGACCTGCT GTCCGAGGTG 
AGTGGGCCCT CCCCACCTCT GGCCCGGGCC TCCTCCTTCT GGGGCCACTT GGGCGCCCAG 
ATGCAGCAGC ATCCCCACCG CCAGAGCAAG ATCTCGAAGC ACATGCCCCC GCCAGCCCCC 
CGCCCAGGCT CAGAAACCAC CTGGAGCAAA GACCCACCCG AGACCAGAAG CAGCTTAGAG 
CTGGACACGG AGCTGAGCTG GATTTCAGGA GACCTCCTGA CCGTGGGCAG CCAGGAGGAG 
CCCCCGTCCC CACGGGACCT GAAGAAGTGT CACAGCGTGG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGCGT CCTGGCTGGA TGAACAGAGG AGACACTCCA TCGCGGTCAG CTGCCTGGAC 
GGCGGCTCCC AACCCAGCCT GGGCCCCGGC CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG 
GGACCTGGAA GCCGGCCCAA GAAAAAACTC AGCCCACCTA GTATCTCCAT AGACCCCCCA 
GAGAGTCAGG GCCCTCGGCC CCCACCCAGC CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG 
TCCAGCGACT CCAGGGACCC CTCGGCCTCC GGCCCCCCTG ACAGCATGGC TGCCTCGCCC 
CCCCCAAAGA AAGACGTGCT GAGTCTCTCT GGTTTAGCCT CTGACCCAGC AGACCTGGAC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624656.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624713.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X21, mRNA.

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CloneID	OCe11925
Clone ID Related Accession (Same CDS sequence)	XM_005624656.2
Accession Version	XM_005624656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X21
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGAGGCCCA GTGTAAACCC 
TACTACTCAG ACTACTCCCG ATTCCGGCTC CTCGTCCACC ACTTGTGTAC CAGCCACTAT 
CTGGACCTCT TCATCACGGG TGTCATTGGT CTGAATGTGG TCACCATGGC CATGGAGCAC 
TACCAGCAGC CCCAGATCCT GGACGAGGCC TTGAAGATCT GCAACTATAT CTTCACCGTC 
ATCTTTGTCT TGGAGTCAGT TTTTAAACTC GTGGCCTTTG GCTTCCGTCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCCATC GTGCTGCTGT CCATCATGGG CATCACGCTG 
GAGGAGATTG AGGTCAATGC CTCGCTGCCC ATCAACCCCA CCATCATCCG CATCATGAGA 
GTGCTGCGCA TTGCCCGAGT GCTGAAGCTG CTGAAGATGG CTGTGGGCAT GCGGGCGCTG 
CTGGACACAG TCATGCAGGC CCTGCCTCAG GTGGGGAACC TGGGACTTCT CTTCATGTTG 
TTGTTTTTCA TCTTTGCAGC TCTGGGTGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACGCACCCCT GCGAGGGCCT GGGCCGGCAT GCCACCTTTC GGAACTTTGG CATGGCCTTC 
CTGACCCTCT TCCGAGTGTC CACAGGTGAC AACTGGAACG GCATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACATGCTAC AATACAGTCA TCTCCCCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TGATCGCTGT GCTGATGAAG 
CACCTGGAGG AAAGCAACAA GGAGGCCAAA GAGGAGGCCG AGCTGGAGGC TGAGCTGGAG 
CTGGAGATGA AGACGCTCAG CCCACAGCCC CACTCGCCAC TGGGCAGCCC CTTCCTCTGG 
CCGGGGGTCG AGGGCCCCGA CAGCCCCGAT AGCCCCAAGC TCAGGGCCCC GCACACCGCG 
GCCCACACGG GAGCAGCCTC GCGCTTCTCC CTGGAACACC CCACGATGGA GCCCCACCCC 
GAGGAGATGC CCCTTGCACT GGGACTGGAC CTGCTGACTG TGAGGAAGTC TGGGGTCAGC 
CGGACACACT CCCTGCCCAA TGACAGCTAC ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG 
TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC AAAGCTCAGT CAGGCTCTGT CTTATCTGTC 
CACTCGCAGC CAGCAGACAC CAGCTACATC CTGCAGCTTC CCAAAGACAC ACCTCACCTG 
CTCCAGCCCC ACAGCGCTCC CACCTGGGGC ACCATTCCCA AACTGCCCCC ACCAGGCCGC 
TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TAAGGACTGA TTCTCTGGAC 
GTTCAGGGCC TGGGCAGCCG GGAAGACCTG CTGTCCGAGG TGAGTGGGCC CTCCCCACCT 
CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC TTGGGCGCCC AGATGCAGCA GCATCCCCAC 
CGCCAGAGCA AGATCTCGAA GCACATGCCC CCGCCAGCCC CCCGCCCAGG CTCAGAAACC 
ACCTGGAGCA AAGACCCACC CGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GACCGTGGGC AGCCAGGAGG AGCCCCCGTC CCCACGGGAC 
CTGAAGAAGT GTCACAGCGT GGAGGCCCAG AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG 
GATGAACAGA GGAGACACTC CATCGCGGTC AGCTGCCTGG ACGGCGGCTC CCAACCCAGC 
CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC CAGCCTCTTG GGGGACCTGG AAGCCGGCCC 
AAGAAAAAAC TCAGCCCACC TAGTATCTCC ATAGACCCCC CAGAGAGTCA GGGCCCTCGG 
CCCCCACCCA GCCCCGGCGT CTGCCTCCGG CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC 
CCCTCGGCCT CCGGCCCCCC TGACAGCATG GCTGCCTCGC CCCCCCCAAA GAAAGACGTG 
CTGAGTCTCT CTGGTTTAGC CTCTGACCCA GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624713.1
CDS	527..7414
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624656.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624656.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGAGGCCCA GTGTAAACCC 
TACTACTCAG ACTACTCCCG ATTCCGGCTC CTCGTCCACC ACTTGTGTAC CAGCCACTAT 
CTGGACCTCT TCATCACGGG TGTCATTGGT CTGAATGTGG TCACCATGGC CATGGAGCAC 
TACCAGCAGC CCCAGATCCT GGACGAGGCC TTGAAGATCT GCAACTATAT CTTCACCGTC 
ATCTTTGTCT TGGAGTCAGT TTTTAAACTC GTGGCCTTTG GCTTCCGTCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCCATC GTGCTGCTGT CCATCATGGG CATCACGCTG 
GAGGAGATTG AGGTCAATGC CTCGCTGCCC ATCAACCCCA CCATCATCCG CATCATGAGA 
GTGCTGCGCA TTGCCCGAGT GCTGAAGCTG CTGAAGATGG CTGTGGGCAT GCGGGCGCTG 
CTGGACACAG TCATGCAGGC CCTGCCTCAG GTGGGGAACC TGGGACTTCT CTTCATGTTG 
TTGTTTTTCA TCTTTGCAGC TCTGGGTGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACGCACCCCT GCGAGGGCCT GGGCCGGCAT GCCACCTTTC GGAACTTTGG CATGGCCTTC 
CTGACCCTCT TCCGAGTGTC CACAGGTGAC AACTGGAACG GCATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACATGCTAC AATACAGTCA TCTCCCCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TGATCGCTGT GCTGATGAAG 
CACCTGGAGG AAAGCAACAA GGAGGCCAAA GAGGAGGCCG AGCTGGAGGC TGAGCTGGAG 
CTGGAGATGA AGACGCTCAG CCCACAGCCC CACTCGCCAC TGGGCAGCCC CTTCCTCTGG 
CCGGGGGTCG AGGGCCCCGA CAGCCCCGAT AGCCCCAAGC TCAGGGCCCC GCACACCGCG 
GCCCACACGG GAGCAGCCTC GCGCTTCTCC CTGGAACACC CCACGATGGA GCCCCACCCC 
GAGGAGATGC CCCTTGCACT GGGACTGGAC CTGCTGACTG TGAGGAAGTC TGGGGTCAGC 
CGGACACACT CCCTGCCCAA TGACAGCTAC ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG 
TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC AAAGCTCAGT CAGGCTCTGT CTTATCTGTC 
CACTCGCAGC CAGCAGACAC CAGCTACATC CTGCAGCTTC CCAAAGACAC ACCTCACCTG 
CTCCAGCCCC ACAGCGCTCC CACCTGGGGC ACCATTCCCA AACTGCCCCC ACCAGGCCGC 
TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TAAGGACTGA TTCTCTGGAC 
GTTCAGGGCC TGGGCAGCCG GGAAGACCTG CTGTCCGAGG TGAGTGGGCC CTCCCCACCT 
CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC TTGGGCGCCC AGATGCAGCA GCATCCCCAC 
CGCCAGAGCA AGATCTCGAA GCACATGCCC CCGCCAGCCC CCCGCCCAGG CTCAGAAACC 
ACCTGGAGCA AAGACCCACC CGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GACCGTGGGC AGCCAGGAGG AGCCCCCGTC CCCACGGGAC 
CTGAAGAAGT GTCACAGCGT GGAGGCCCAG AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG 
GATGAACAGA GGAGACACTC CATCGCGGTC AGCTGCCTGG ACGGCGGCTC CCAACCCAGC 
CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC CAGCCTCTTG GGGGACCTGG AAGCCGGCCC 
AAGAAAAAAC TCAGCCCACC TAGTATCTCC ATAGACCCCC CAGAGAGTCA GGGCCCTCGG 
CCCCCACCCA GCCCCGGCGT CTGCCTCCGG CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC 
CCCTCGGCCT CCGGCCCCCC TGACAGCATG GCTGCCTCGC CCCCCCCAAA GAAAGACGTG 
CTGAGTCTCT CTGGTTTAGC CTCTGACCCA GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624657.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624714.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X22, mRNA.

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CloneID	OCe11926
Clone ID Related Accession (Same CDS sequence)	XM_005624657.2 , XM_005624657.3
Accession Version	XM_005624657.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X22
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGATCT AATGCTGGAC 
GATGTAATTG CTTCCGGCAG CTCATCCAGC GCGGCGCCAG AGGCCCAGTG TAAACCCTAC 
TACTCAGACT ACTCCCGATT CCGGCTCCTC GTCCACCACT TGTGTACCAG CCACTATCTG 
GACCTCTTCA TCACGGGTGT CATTGGTCTG AATGTGGTCA CCATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCCTTG AAGATCTGCA ACTATATCTT CACCGTCATC 
TTTGTCTTGG AGTCAGTTTT TAAACTCGTG GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATCGTG CTGCTGTCCA TCATGGGCAT CACGCTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGAGTG 
CTGCGCATTG CCCGAGTGCT GAAGCTGCTG AAGATGGCTG TGGGCATGCG GGCGCTGCTG 
GACACAGTCA TGCAGGCCCT GCCTCAGGTG GGGAACCTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGTGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG 
ACCCTCTTCC GAGTGTCCAC AGGTGACAAC TGGAACGGCA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC ATGCTACAAT ACAGTCATCT CCCCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTGA TCGCTGTGCT GATGAAGCAC 
CTGGAGGAAA GCAACAAGGA GGCCAAAGAG GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG 
GAGATGAAGA CGCTCAGCCC ACAGCCCCAC TCGCCACTGG GCAGCCCCTT CCTCTGGCCG 
GGGGTCGAGG GCCCCGACAG CCCCGATAGC CCCAAGCTCA GGGCCCCGCA CACCGCGGCC 
CACACGGGAG CAGCCTCGCG CTTCTCCCTG GAACACCCCA CGATGGAGCC CCACCCCGAG 
GAGATGCCCC TTGCACTGGG ACTGGACCTG CTGACTGTGA GGAAGTCTGG GGTCAGCCGG 
ACACACTCCC TGCCCAATGA CAGCTACATG TTCCGGGACG GAGGCACCGC TGAGGGGTCC 
CTAGGATGCA GAAGCTGGGG GCTCCCCAAA GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC 
TCGCAGCCAG CAGACACCAG CTACATCCTG CAGCTTCCCA AAGACACACC TCACCTGCTC 
CAGCCCCACA GCGCTCCCAC CTGGGGCACC ATTCCCAAAC TGCCCCCACC AGGCCGCTCC 
CCCTTGGCTC AGAGGCCACT CAGGCGCCAG GCAGCAATAA GGACTGATTC TCTGGACGTT 
CAGGGCCTGG GCAGCCGGGA AGACCTGCTG TCCGAGGTGA GTGGGCCCTC CCCACCTCTG 
GCCCGGGCCT CCTCCTTCTG GGGCCACTTG GGCGCCCAGA TGCAGCAGCA TCCCCACCGC 
CAGAGCAAGA TCTCGAAGCA CATGCCCCCG CCAGCCCCCC GCCCAGGCTC AGAAACCACC 
TGGAGCAAAG ACCCACCCGA GACCAGAAGC AGCTTAGAGC TGGACACGGA GCTGAGCTGG 
ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC CAGGAGGAGC CCCCGTCCCC ACGGGACCTG 
AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT 
GAACAGAGGA GACACTCCAT CGCGGTCAGC TGCCTGGACG GCGGCTCCCA ACCCAGCCTG 
GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG CCTCTTGGGG GACCTGGAAG CCGGCCCAAG 
AAAAAACTCA GCCCACCTAG TATCTCCATA GACCCCCCAG AGAGTCAGGG CCCTCGGCCC 
CCACCCAGCC CCGGCGTCTG CCTCCGGCGG AGGGCTCCGT CCAGCGACTC CAGGGACCCC 
TCGGCCTCCG GCCCCCCTGA CAGCATGGCT GCCTCGCCCC CCCCAAAGAA AGACGTGCTG 
AGTCTCTCTG GTTTAGCCTC TGACCCAGCA GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624714.1
CDS	526..7410
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624657.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624657.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGATCT AATGCTGGAC 
GATGTAATTG CTTCCGGCAG CTCATCCAGC GCGGCGCCAG AGGCCCAGTG TAAACCCTAC 
TACTCAGACT ACTCCCGATT CCGGCTCCTC GTCCACCACT TGTGTACCAG CCACTATCTG 
GACCTCTTCA TCACGGGTGT CATTGGTCTG AATGTGGTCA CCATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCCTTG AAGATCTGCA ACTATATCTT CACCGTCATC 
TTTGTCTTGG AGTCAGTTTT TAAACTCGTG GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATCGTG CTGCTGTCCA TCATGGGCAT CACGCTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGAGTG 
CTGCGCATTG CCCGAGTGCT GAAGCTGCTG AAGATGGCTG TGGGCATGCG GGCGCTGCTG 
GACACAGTCA TGCAGGCCCT GCCTCAGGTG GGGAACCTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGTGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG 
ACCCTCTTCC GAGTGTCCAC AGGTGACAAC TGGAACGGCA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC ATGCTACAAT ACAGTCATCT CCCCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTGA TCGCTGTGCT GATGAAGCAC 
CTGGAGGAAA GCAACAAGGA GGCCAAAGAG GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG 
GAGATGAAGA CGCTCAGCCC ACAGCCCCAC TCGCCACTGG GCAGCCCCTT CCTCTGGCCG 
GGGGTCGAGG GCCCCGACAG CCCCGATAGC CCCAAGCTCA GGGCCCCGCA CACCGCGGCC 
CACACGGGAG CAGCCTCGCG CTTCTCCCTG GAACACCCCA CGATGGAGCC CCACCCCGAG 
GAGATGCCCC TTGCACTGGG ACTGGACCTG CTGACTGTGA GGAAGTCTGG GGTCAGCCGG 
ACACACTCCC TGCCCAATGA CAGCTACATG TTCCGGGACG GAGGCACCGC TGAGGGGTCC 
CTAGGATGCA GAAGCTGGGG GCTCCCCAAA GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC 
TCGCAGCCAG CAGACACCAG CTACATCCTG CAGCTTCCCA AAGACACACC TCACCTGCTC 
CAGCCCCACA GCGCTCCCAC CTGGGGCACC ATTCCCAAAC TGCCCCCACC AGGCCGCTCC 
CCCTTGGCTC AGAGGCCACT CAGGCGCCAG GCAGCAATAA GGACTGATTC TCTGGACGTT 
CAGGGCCTGG GCAGCCGGGA AGACCTGCTG TCCGAGGTGA GTGGGCCCTC CCCACCTCTG 
GCCCGGGCCT CCTCCTTCTG GGGCCACTTG GGCGCCCAGA TGCAGCAGCA TCCCCACCGC 
CAGAGCAAGA TCTCGAAGCA CATGCCCCCG CCAGCCCCCC GCCCAGGCTC AGAAACCACC 
TGGAGCAAAG ACCCACCCGA GACCAGAAGC AGCTTAGAGC TGGACACGGA GCTGAGCTGG 
ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC CAGGAGGAGC CCCCGTCCCC ACGGGACCTG 
AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT 
GAACAGAGGA GACACTCCAT CGCGGTCAGC TGCCTGGACG GCGGCTCCCA ACCCAGCCTG 
GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG CCTCTTGGGG GACCTGGAAG CCGGCCCAAG 
AAAAAACTCA GCCCACCTAG TATCTCCATA GACCCCCCAG AGAGTCAGGG CCCTCGGCCC 
CCACCCAGCC CCGGCGTCTG CCTCCGGCGG AGGGCTCCGT CCAGCGACTC CAGGGACCCC 
TCGGCCTCCG GCCCCCCTGA CAGCATGGCT GCCTCGCCCC CCCCAAAGAA AGACGTGCTG 
AGTCTCTCTG GTTTAGCCTC TGACCCAGCA GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624657.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624714.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X18, mRNA.

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CloneID	OCe11926
Clone ID Related Accession (Same CDS sequence)	XM_005624657.2 , XM_005624657.3
Accession Version	XM_005624657.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624657.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGATCT AATGCTGGAC 
GATGTAATTG CTTCCGGCAG CTCATCCAGC GCGGCGCCAG AGGCCCAGTG TAAACCCTAC 
TACTCAGACT ACTCCCGATT CCGGCTCCTC GTCCACCACT TGTGTACCAG CCACTATCTG 
GACCTCTTCA TCACGGGTGT CATTGGTCTG AATGTGGTCA CCATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCCTTG AAGATCTGCA ACTATATCTT CACCGTCATC 
TTTGTCTTGG AGTCAGTTTT TAAACTCGTG GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATCGTG CTGCTGTCCA TCATGGGCAT CACGCTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGAGTG 
CTGCGCATTG CCCGAGTGCT GAAGCTGCTG AAGATGGCTG TGGGCATGCG GGCGCTGCTG 
GACACAGTCA TGCAGGCCCT GCCTCAGGTG GGGAACCTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGTGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG 
ACCCTCTTCC GAGTGTCCAC AGGTGACAAC TGGAACGGCA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC ATGCTACAAT ACAGTCATCT CCCCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTGA TCGCTGTGCT GATGAAGCAC 
CTGGAGGAAA GCAACAAGGA GGCCAAAGAG GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG 
GAGATGAAGA CGCTCAGCCC ACAGCCCCAC TCGCCACTGG GCAGCCCCTT CCTCTGGCCG 
GGGGTCGAGG GCCCCGACAG CCCCGATAGC CCCAAGCTCA GGGCCCCGCA CACCGCGGCC 
CACACGGGAG CAGCCTCGCG CTTCTCCCTG GAACACCCCA CGATGGAGCC CCACCCCGAG 
GAGATGCCCC TTGCACTGGG ACTGGACCTG CTGACTGTGA GGAAGTCTGG GGTCAGCCGG 
ACACACTCCC TGCCCAATGA CAGCTACATG TTCCGGGACG GAGGCACCGC TGAGGGGTCC 
CTAGGATGCA GAAGCTGGGG GCTCCCCAAA GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC 
TCGCAGCCAG CAGACACCAG CTACATCCTG CAGCTTCCCA AAGACACACC TCACCTGCTC 
CAGCCCCACA GCGCTCCCAC CTGGGGCACC ATTCCCAAAC TGCCCCCACC AGGCCGCTCC 
CCCTTGGCTC AGAGGCCACT CAGGCGCCAG GCAGCAATAA GGACTGATTC TCTGGACGTT 
CAGGGCCTGG GCAGCCGGGA AGACCTGCTG TCCGAGGTGA GTGGGCCCTC CCCACCTCTG 
GCCCGGGCCT CCTCCTTCTG GGGCCACTTG GGCGCCCAGA TGCAGCAGCA TCCCCACCGC 
CAGAGCAAGA TCTCGAAGCA CATGCCCCCG CCAGCCCCCC GCCCAGGCTC AGAAACCACC 
TGGAGCAAAG ACCCACCCGA GACCAGAAGC AGCTTAGAGC TGGACACGGA GCTGAGCTGG 
ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC CAGGAGGAGC CCCCGTCCCC ACGGGACCTG 
AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT 
GAACAGAGGA GACACTCCAT CGCGGTCAGC TGCCTGGACG GCGGCTCCCA ACCCAGCCTG 
GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG CCTCTTGGGG GACCTGGAAG CCGGCCCAAG 
AAAAAACTCA GCCCACCTAG TATCTCCATA GACCCCCCAG AGAGTCAGGG CCCTCGGCCC 
CCACCCAGCC CCGGCGTCTG CCTCCGGCGG AGGGCTCCGT CCAGCGACTC CAGGGACCCC 
TCGGCCTCCG GCCCCCCTGA CAGCATGGCT GCCTCGCCCC CCCCAAAGAA AGACGTGCTG 
AGTCTCTCTG GTTTAGCCTC TGACCCAGCA GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624714.1
CDS	504..7388
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624657.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624657.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGATCT AATGCTGGAC 
GATGTAATTG CTTCCGGCAG CTCATCCAGC GCGGCGCCAG AGGCCCAGTG TAAACCCTAC 
TACTCAGACT ACTCCCGATT CCGGCTCCTC GTCCACCACT TGTGTACCAG CCACTATCTG 
GACCTCTTCA TCACGGGTGT CATTGGTCTG AATGTGGTCA CCATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCCTTG AAGATCTGCA ACTATATCTT CACCGTCATC 
TTTGTCTTGG AGTCAGTTTT TAAACTCGTG GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATCGTG CTGCTGTCCA TCATGGGCAT CACGCTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGAGTG 
CTGCGCATTG CCCGAGTGCT GAAGCTGCTG AAGATGGCTG TGGGCATGCG GGCGCTGCTG 
GACACAGTCA TGCAGGCCCT GCCTCAGGTG GGGAACCTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGTGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG 
ACCCTCTTCC GAGTGTCCAC AGGTGACAAC TGGAACGGCA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC ATGCTACAAT ACAGTCATCT CCCCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTGA TCGCTGTGCT GATGAAGCAC 
CTGGAGGAAA GCAACAAGGA GGCCAAAGAG GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG 
GAGATGAAGA CGCTCAGCCC ACAGCCCCAC TCGCCACTGG GCAGCCCCTT CCTCTGGCCG 
GGGGTCGAGG GCCCCGACAG CCCCGATAGC CCCAAGCTCA GGGCCCCGCA CACCGCGGCC 
CACACGGGAG CAGCCTCGCG CTTCTCCCTG GAACACCCCA CGATGGAGCC CCACCCCGAG 
GAGATGCCCC TTGCACTGGG ACTGGACCTG CTGACTGTGA GGAAGTCTGG GGTCAGCCGG 
ACACACTCCC TGCCCAATGA CAGCTACATG TTCCGGGACG GAGGCACCGC TGAGGGGTCC 
CTAGGATGCA GAAGCTGGGG GCTCCCCAAA GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC 
TCGCAGCCAG CAGACACCAG CTACATCCTG CAGCTTCCCA AAGACACACC TCACCTGCTC 
CAGCCCCACA GCGCTCCCAC CTGGGGCACC ATTCCCAAAC TGCCCCCACC AGGCCGCTCC 
CCCTTGGCTC AGAGGCCACT CAGGCGCCAG GCAGCAATAA GGACTGATTC TCTGGACGTT 
CAGGGCCTGG GCAGCCGGGA AGACCTGCTG TCCGAGGTGA GTGGGCCCTC CCCACCTCTG 
GCCCGGGCCT CCTCCTTCTG GGGCCACTTG GGCGCCCAGA TGCAGCAGCA TCCCCACCGC 
CAGAGCAAGA TCTCGAAGCA CATGCCCCCG CCAGCCCCCC GCCCAGGCTC AGAAACCACC 
TGGAGCAAAG ACCCACCCGA GACCAGAAGC AGCTTAGAGC TGGACACGGA GCTGAGCTGG 
ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC CAGGAGGAGC CCCCGTCCCC ACGGGACCTG 
AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT 
GAACAGAGGA GACACTCCAT CGCGGTCAGC TGCCTGGACG GCGGCTCCCA ACCCAGCCTG 
GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG CCTCTTGGGG GACCTGGAAG CCGGCCCAAG 
AAAAAACTCA GCCCACCTAG TATCTCCATA GACCCCCCAG AGAGTCAGGG CCCTCGGCCC 
CCACCCAGCC CCGGCGTCTG CCTCCGGCGG AGGGCTCCGT CCAGCGACTC CAGGGACCCC 
TCGGCCTCCG GCCCCCCTGA CAGCATGGCT GCCTCGCCCC CCCCAAAGAA AGACGTGCTG 
AGTCTCTCTG GTTTAGCCTC TGACCCAGCA GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860610.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865703.2 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X24, mRNA.

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CloneID	OCe11927
Clone ID Related Accession (Same CDS sequence)	XM_860610.4 , XM_860610.5
Accession Version	XM_860610.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X24
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_860610.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GATGGAGCCC CACCCCGAGG AGATGCCCCT TGCACTGGGA 
CTGGACCTGC TGACTGTGAG GAAGTCTGGG GTCAGCCGGA CACACTCCCT GCCCAATGAC 
AGCTACATGT TCCGGGACGG AGGCACCGCT GAGGGGTCCC TAGGATGCAG AAGCTGGGGG 
CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA TCTGTCCACT CGCAGCCAGC AGACACCAGC 
TACATCCTGC AGCTTCCCAA AGACACACCT CACCTGCTCC AGCCCCACAG CGCTCCCACC 
TGGGGCACCA TTCCCAAACT GCCCCCACCA GGCCGCTCCC CCTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATAAG GACTGATTCT CTGGACGTTC AGGGCCTGGG CAGCCGGGAA 
GACCTGCTGT CCGAGGTGAG TGGGCCCTCC CCACCTCTGG CCCGGGCCTC CTCCTTCTGG 
GGCCACTTGG GCGCCCAGAT GCAGCAGCAT CCCCACCGCC AGAGCAAGAT CTCGAAGCAC 
ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA GAAACCACCT GGAGCAAAGA CCCACCCGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGACC 
GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA CGGGACCTGA AGAAGTGTCA CAGCGTGGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC TGGCTGGATG AACAGAGGAG ACACTCCATC 
GCGGTCAGCT GCCTGGACGG CGGCTCCCAA CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT 
GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC CGGCCCAAGA AAAAACTCAG CCCACCTAGT 
ATCTCCATAG ACCCCCCAGA GAGTCAGGGC CCTCGGCCCC CACCCAGCCC CGGCGTCTGC 
CTCCGGCGGA GGGCTCCGTC CAGCGACTCC AGGGACCCCT CGGCCTCCGG CCCCCCTGAC 
AGCATGGCTG CCTCGCCCCC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTAGCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865703.2
CDS	526..7389
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860610.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860610.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GATGGAGCCC CACCCCGAGG AGATGCCCCT TGCACTGGGA 
CTGGACCTGC TGACTGTGAG GAAGTCTGGG GTCAGCCGGA CACACTCCCT GCCCAATGAC 
AGCTACATGT TCCGGGACGG AGGCACCGCT GAGGGGTCCC TAGGATGCAG AAGCTGGGGG 
CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA TCTGTCCACT CGCAGCCAGC AGACACCAGC 
TACATCCTGC AGCTTCCCAA AGACACACCT CACCTGCTCC AGCCCCACAG CGCTCCCACC 
TGGGGCACCA TTCCCAAACT GCCCCCACCA GGCCGCTCCC CCTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATAAG GACTGATTCT CTGGACGTTC AGGGCCTGGG CAGCCGGGAA 
GACCTGCTGT CCGAGGTGAG TGGGCCCTCC CCACCTCTGG CCCGGGCCTC CTCCTTCTGG 
GGCCACTTGG GCGCCCAGAT GCAGCAGCAT CCCCACCGCC AGAGCAAGAT CTCGAAGCAC 
ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA GAAACCACCT GGAGCAAAGA CCCACCCGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGACC 
GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA CGGGACCTGA AGAAGTGTCA CAGCGTGGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC TGGCTGGATG AACAGAGGAG ACACTCCATC 
GCGGTCAGCT GCCTGGACGG CGGCTCCCAA CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT 
GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC CGGCCCAAGA AAAAACTCAG CCCACCTAGT 
ATCTCCATAG ACCCCCCAGA GAGTCAGGGC CCTCGGCCCC CACCCAGCCC CGGCGTCTGC 
CTCCGGCGGA GGGCTCCGTC CAGCGACTCC AGGGACCCCT CGGCCTCCGG CCCCCCTGAC 
AGCATGGCTG CCTCGCCCCC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTAGCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860610.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865703.2 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X20, mRNA.

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CloneID	OCe11927
Clone ID Related Accession (Same CDS sequence)	XM_860610.4 , XM_860610.5
Accession Version	XM_860610.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_860610.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GATGGAGCCC CACCCCGAGG AGATGCCCCT TGCACTGGGA 
CTGGACCTGC TGACTGTGAG GAAGTCTGGG GTCAGCCGGA CACACTCCCT GCCCAATGAC 
AGCTACATGT TCCGGGACGG AGGCACCGCT GAGGGGTCCC TAGGATGCAG AAGCTGGGGG 
CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA TCTGTCCACT CGCAGCCAGC AGACACCAGC 
TACATCCTGC AGCTTCCCAA AGACACACCT CACCTGCTCC AGCCCCACAG CGCTCCCACC 
TGGGGCACCA TTCCCAAACT GCCCCCACCA GGCCGCTCCC CCTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATAAG GACTGATTCT CTGGACGTTC AGGGCCTGGG CAGCCGGGAA 
GACCTGCTGT CCGAGGTGAG TGGGCCCTCC CCACCTCTGG CCCGGGCCTC CTCCTTCTGG 
GGCCACTTGG GCGCCCAGAT GCAGCAGCAT CCCCACCGCC AGAGCAAGAT CTCGAAGCAC 
ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA GAAACCACCT GGAGCAAAGA CCCACCCGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGACC 
GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA CGGGACCTGA AGAAGTGTCA CAGCGTGGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC TGGCTGGATG AACAGAGGAG ACACTCCATC 
GCGGTCAGCT GCCTGGACGG CGGCTCCCAA CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT 
GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC CGGCCCAAGA AAAAACTCAG CCCACCTAGT 
ATCTCCATAG ACCCCCCAGA GAGTCAGGGC CCTCGGCCCC CACCCAGCCC CGGCGTCTGC 
CTCCGGCGGA GGGCTCCGTC CAGCGACTCC AGGGACCCCT CGGCCTCCGG CCCCCCTGAC 
AGCATGGCTG CCTCGCCCCC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTAGCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865703.2
CDS	504..7367
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860610.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860610.5

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GATGGAGCCC CACCCCGAGG AGATGCCCCT TGCACTGGGA 
CTGGACCTGC TGACTGTGAG GAAGTCTGGG GTCAGCCGGA CACACTCCCT GCCCAATGAC 
AGCTACATGT TCCGGGACGG AGGCACCGCT GAGGGGTCCC TAGGATGCAG AAGCTGGGGG 
CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA TCTGTCCACT CGCAGCCAGC AGACACCAGC 
TACATCCTGC AGCTTCCCAA AGACACACCT CACCTGCTCC AGCCCCACAG CGCTCCCACC 
TGGGGCACCA TTCCCAAACT GCCCCCACCA GGCCGCTCCC CCTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATAAG GACTGATTCT CTGGACGTTC AGGGCCTGGG CAGCCGGGAA 
GACCTGCTGT CCGAGGTGAG TGGGCCCTCC CCACCTCTGG CCCGGGCCTC CTCCTTCTGG 
GGCCACTTGG GCGCCCAGAT GCAGCAGCAT CCCCACCGCC AGAGCAAGAT CTCGAAGCAC 
ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA GAAACCACCT GGAGCAAAGA CCCACCCGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGACC 
GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA CGGGACCTGA AGAAGTGTCA CAGCGTGGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC TGGCTGGATG AACAGAGGAG ACACTCCATC 
GCGGTCAGCT GCCTGGACGG CGGCTCCCAA CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT 
GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC CGGCCCAAGA AAAAACTCAG CCCACCTAGT 
ATCTCCATAG ACCCCCCAGA GAGTCAGGGC CCTCGGCCCC CACCCAGCCC CGGCGTCTGC 
CTCCGGCGGA GGGCTCCGTC CAGCGACTCC AGGGACCCCT CGGCCTCCGG CCCCCCTGAC 
AGCATGGCTG CCTCGCCCCC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTAGCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_003435267.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_003435315.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X26, mRNA.

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CloneID	OCe11929
Clone ID Related Accession (Same CDS sequence)	XM_003435267.3 , XM_003435267.4
Accession Version	XM_003435267.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X26
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003435267.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGAGGC CCAGTGTAAA 
CCCTACTACT CAGACTACTC CCGATTCCGG CTCCTCGTCC ACCACTTGTG TACCAGCCAC 
TATCTGGACC TCTTCATCAC GGGTGTCATT GGTCTGAATG TGGTCACCAT GGCCATGGAG 
CACTACCAGC AGCCCCAGAT CCTGGACGAG GCCTTGAAGA TCTGCAACTA TATCTTCACC 
GTCATCTTTG TCTTGGAGTC AGTTTTTAAA CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCC ATCGTGCTGC TGTCCATCAT GGGCATCACG 
CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG CCCATCAACC CCACCATCAT CCGCATCATG 
AGAGTGCTGC GCATTGCCCG AGTGCTGAAG CTGCTGAAGA TGGCTGTGGG CATGCGGGCG 
CTGCTGGACA CAGTCATGCA GGCCCTGCCT CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
TTGTTGTTTT TCATCTTTGC AGCTCTGGGT GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACGCACC CCTGCGAGGG CCTGGGCCGG CATGCCACCT TTCGGAACTT TGGCATGGCC 
TTCCTGACCC TCTTCCGAGT GTCCACAGGT GACAACTGGA ACGGCATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACATGC TACAATACAG TCATCTCCCC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTGATCGC TGTGCTGATG 
AAGCACCTGG AGGAAAGCAA CAAGGAGGCC AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG 
GAGCTGGAGA TGAAGACGCT CAGCCCACAG CCCCACTCGC CACTGGGCAG CCCCTTCCTC 
TGGCCGGGGG TCGAGGGCCC CGACAGCCCC GATAGCCCCA AGCTCAGGGC CCCGCACACC 
GCGGCCCACA CGGGAGCAGC CTCGCGCTTC TCCCTGGAAC ACCCCACGAT GGAGCCCCAC 
CCCGAGGAGA TGCCCCTTGC ACTGGGACTG GACCTGCTGA CTGTGAGGAA GTCTGGGGTC 
AGCCGGACAC ACTCCCTGCC CAATGACAGC TACATGTTCC GGGACGGAGG CACCGCTGAG 
GGGTCCCTAG GATGCAGAAG CTGGGGGCTC CCCAAAGCTC AGTCAGGCTC TGTCTTATCT 
GTCCACTCGC AGCCAGCAGA CACCAGCTAC ATCCTGCAGC TTCCCAAAGA CACACCTCAC 
CTGCTCCAGC CCCACAGCGC TCCCACCTGG GGCACCATTC CCAAACTGCC CCCACCAGGC 
CGCTCCCCCT TGGCTCAGAG GCCACTCAGG CGCCAGGCAG CAATAAGGAC TGATTCTCTG 
GACGTTCAGG GCCTGGGCAG CCGGGAAGAC CTGCTGTCCG AGGTGAGTGG GCCCTCCCCA 
CCTCTGGCCC GGGCCTCCTC CTTCTGGGGC CACTTGGGCG CCCAGATGCA GCAGCATCCC 
CACCGCCAGA GCAAGATCTC GAAGCACATG CCCCCGCCAG CCCCCCGCCC AGGCTCAGAA 
ACCACCTGGA GCAAAGACCC ACCCGAGACC AGAAGCAGCT TAGAGCTGGA CACGGAGCTG 
AGCTGGATTT CAGGAGACCT CCTGACCGTG GGCAGCCAGG AGGAGCCCCC GTCCCCACGG 
GACCTGAAGA AGTGTCACAG CGTGGAGGCC CAGAGCTGCC GGCGCAGGCC TGCGTCCTGG 
CTGGATGAAC AGAGGAGACA CTCCATCGCG GTCAGCTGCC TGGACGGCGG CTCCCAACCC 
AGCCTGGGCC CCGGCCCCTC TAGCCTTGGC GGCCAGCCTC TTGGGGGACC TGGAAGCCGG 
CCCAAGAAAA AACTCAGCCC ACCTAGTATC TCCATAGACC CCCCAGAGAG TCAGGGCCCT 
CGGCCCCCAC CCAGCCCCGG CGTCTGCCTC CGGCGGAGGG CTCCGTCCAG CGACTCCAGG 
GACCCCTCGG CCTCCGGCCC CCCTGACAGC ATGGCTGCCT CGCCCCCCCC AAAGAAAGAC 
GTGCTGAGTC TCTCTGGTTT AGCCTCTGAC CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435315.1
CDS	526..7356
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_003435267.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003435267.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGAGGC CCAGTGTAAA 
CCCTACTACT CAGACTACTC CCGATTCCGG CTCCTCGTCC ACCACTTGTG TACCAGCCAC 
TATCTGGACC TCTTCATCAC GGGTGTCATT GGTCTGAATG TGGTCACCAT GGCCATGGAG 
CACTACCAGC AGCCCCAGAT CCTGGACGAG GCCTTGAAGA TCTGCAACTA TATCTTCACC 
GTCATCTTTG TCTTGGAGTC AGTTTTTAAA CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCC ATCGTGCTGC TGTCCATCAT GGGCATCACG 
CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG CCCATCAACC CCACCATCAT CCGCATCATG 
AGAGTGCTGC GCATTGCCCG AGTGCTGAAG CTGCTGAAGA TGGCTGTGGG CATGCGGGCG 
CTGCTGGACA CAGTCATGCA GGCCCTGCCT CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
TTGTTGTTTT TCATCTTTGC AGCTCTGGGT GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACGCACC CCTGCGAGGG CCTGGGCCGG CATGCCACCT TTCGGAACTT TGGCATGGCC 
TTCCTGACCC TCTTCCGAGT GTCCACAGGT GACAACTGGA ACGGCATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACATGC TACAATACAG TCATCTCCCC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTGATCGC TGTGCTGATG 
AAGCACCTGG AGGAAAGCAA CAAGGAGGCC AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG 
GAGCTGGAGA TGAAGACGCT CAGCCCACAG CCCCACTCGC CACTGGGCAG CCCCTTCCTC 
TGGCCGGGGG TCGAGGGCCC CGACAGCCCC GATAGCCCCA AGCTCAGGGC CCCGCACACC 
GCGGCCCACA CGGGAGCAGC CTCGCGCTTC TCCCTGGAAC ACCCCACGAT GGAGCCCCAC 
CCCGAGGAGA TGCCCCTTGC ACTGGGACTG GACCTGCTGA CTGTGAGGAA GTCTGGGGTC 
AGCCGGACAC ACTCCCTGCC CAATGACAGC TACATGTTCC GGGACGGAGG CACCGCTGAG 
GGGTCCCTAG GATGCAGAAG CTGGGGGCTC CCCAAAGCTC AGTCAGGCTC TGTCTTATCT 
GTCCACTCGC AGCCAGCAGA CACCAGCTAC ATCCTGCAGC TTCCCAAAGA CACACCTCAC 
CTGCTCCAGC CCCACAGCGC TCCCACCTGG GGCACCATTC CCAAACTGCC CCCACCAGGC 
CGCTCCCCCT TGGCTCAGAG GCCACTCAGG CGCCAGGCAG CAATAAGGAC TGATTCTCTG 
GACGTTCAGG GCCTGGGCAG CCGGGAAGAC CTGCTGTCCG AGGTGAGTGG GCCCTCCCCA 
CCTCTGGCCC GGGCCTCCTC CTTCTGGGGC CACTTGGGCG CCCAGATGCA GCAGCATCCC 
CACCGCCAGA GCAAGATCTC GAAGCACATG CCCCCGCCAG CCCCCCGCCC AGGCTCAGAA 
ACCACCTGGA GCAAAGACCC ACCCGAGACC AGAAGCAGCT TAGAGCTGGA CACGGAGCTG 
AGCTGGATTT CAGGAGACCT CCTGACCGTG GGCAGCCAGG AGGAGCCCCC GTCCCCACGG 
GACCTGAAGA AGTGTCACAG CGTGGAGGCC CAGAGCTGCC GGCGCAGGCC TGCGTCCTGG 
CTGGATGAAC AGAGGAGACA CTCCATCGCG GTCAGCTGCC TGGACGGCGG CTCCCAACCC 
AGCCTGGGCC CCGGCCCCTC TAGCCTTGGC GGCCAGCCTC TTGGGGGACC TGGAAGCCGG 
CCCAAGAAAA AACTCAGCCC ACCTAGTATC TCCATAGACC CCCCAGAGAG TCAGGGCCCT 
CGGCCCCCAC CCAGCCCCGG CGTCTGCCTC CGGCGGAGGG CTCCGTCCAG CGACTCCAGG 
GACCCCTCGG CCTCCGGCCC CCCTGACAGC ATGGCTGCCT CGCCCCCCCC AAAGAAAGAC 
GTGCTGAGTC TCTCTGGTTT AGCCTCTGAC CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_003435267.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_003435315.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X22, mRNA.

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CloneID	OCe11929
Clone ID Related Accession (Same CDS sequence)	XM_003435267.3 , XM_003435267.4
Accession Version	XM_003435267.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003435267.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGAGGC CCAGTGTAAA 
CCCTACTACT CAGACTACTC CCGATTCCGG CTCCTCGTCC ACCACTTGTG TACCAGCCAC 
TATCTGGACC TCTTCATCAC GGGTGTCATT GGTCTGAATG TGGTCACCAT GGCCATGGAG 
CACTACCAGC AGCCCCAGAT CCTGGACGAG GCCTTGAAGA TCTGCAACTA TATCTTCACC 
GTCATCTTTG TCTTGGAGTC AGTTTTTAAA CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCC ATCGTGCTGC TGTCCATCAT GGGCATCACG 
CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG CCCATCAACC CCACCATCAT CCGCATCATG 
AGAGTGCTGC GCATTGCCCG AGTGCTGAAG CTGCTGAAGA TGGCTGTGGG CATGCGGGCG 
CTGCTGGACA CAGTCATGCA GGCCCTGCCT CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
TTGTTGTTTT TCATCTTTGC AGCTCTGGGT GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACGCACC CCTGCGAGGG CCTGGGCCGG CATGCCACCT TTCGGAACTT TGGCATGGCC 
TTCCTGACCC TCTTCCGAGT GTCCACAGGT GACAACTGGA ACGGCATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACATGC TACAATACAG TCATCTCCCC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTGATCGC TGTGCTGATG 
AAGCACCTGG AGGAAAGCAA CAAGGAGGCC AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG 
GAGCTGGAGA TGAAGACGCT CAGCCCACAG CCCCACTCGC CACTGGGCAG CCCCTTCCTC 
TGGCCGGGGG TCGAGGGCCC CGACAGCCCC GATAGCCCCA AGCTCAGGGC CCCGCACACC 
GCGGCCCACA CGGGAGCAGC CTCGCGCTTC TCCCTGGAAC ACCCCACGAT GGAGCCCCAC 
CCCGAGGAGA TGCCCCTTGC ACTGGGACTG GACCTGCTGA CTGTGAGGAA GTCTGGGGTC 
AGCCGGACAC ACTCCCTGCC CAATGACAGC TACATGTTCC GGGACGGAGG CACCGCTGAG 
GGGTCCCTAG GATGCAGAAG CTGGGGGCTC CCCAAAGCTC AGTCAGGCTC TGTCTTATCT 
GTCCACTCGC AGCCAGCAGA CACCAGCTAC ATCCTGCAGC TTCCCAAAGA CACACCTCAC 
CTGCTCCAGC CCCACAGCGC TCCCACCTGG GGCACCATTC CCAAACTGCC CCCACCAGGC 
CGCTCCCCCT TGGCTCAGAG GCCACTCAGG CGCCAGGCAG CAATAAGGAC TGATTCTCTG 
GACGTTCAGG GCCTGGGCAG CCGGGAAGAC CTGCTGTCCG AGGTGAGTGG GCCCTCCCCA 
CCTCTGGCCC GGGCCTCCTC CTTCTGGGGC CACTTGGGCG CCCAGATGCA GCAGCATCCC 
CACCGCCAGA GCAAGATCTC GAAGCACATG CCCCCGCCAG CCCCCCGCCC AGGCTCAGAA 
ACCACCTGGA GCAAAGACCC ACCCGAGACC AGAAGCAGCT TAGAGCTGGA CACGGAGCTG 
AGCTGGATTT CAGGAGACCT CCTGACCGTG GGCAGCCAGG AGGAGCCCCC GTCCCCACGG 
GACCTGAAGA AGTGTCACAG CGTGGAGGCC CAGAGCTGCC GGCGCAGGCC TGCGTCCTGG 
CTGGATGAAC AGAGGAGACA CTCCATCGCG GTCAGCTGCC TGGACGGCGG CTCCCAACCC 
AGCCTGGGCC CCGGCCCCTC TAGCCTTGGC GGCCAGCCTC TTGGGGGACC TGGAAGCCGG 
CCCAAGAAAA AACTCAGCCC ACCTAGTATC TCCATAGACC CCCCAGAGAG TCAGGGCCCT 
CGGCCCCCAC CCAGCCCCGG CGTCTGCCTC CGGCGGAGGG CTCCGTCCAG CGACTCCAGG 
GACCCCTCGG CCTCCGGCCC CCCTGACAGC ATGGCTGCCT CGCCCCCCCC AAAGAAAGAC 
GTGCTGAGTC TCTCTGGTTT AGCCTCTGAC CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435315.1
CDS	504..7334
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_003435267.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003435267.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAGTAAG GAGAAGCAGA TGGCTGAGGC CCAGTGTAAA 
CCCTACTACT CAGACTACTC CCGATTCCGG CTCCTCGTCC ACCACTTGTG TACCAGCCAC 
TATCTGGACC TCTTCATCAC GGGTGTCATT GGTCTGAATG TGGTCACCAT GGCCATGGAG 
CACTACCAGC AGCCCCAGAT CCTGGACGAG GCCTTGAAGA TCTGCAACTA TATCTTCACC 
GTCATCTTTG TCTTGGAGTC AGTTTTTAAA CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCC ATCGTGCTGC TGTCCATCAT GGGCATCACG 
CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG CCCATCAACC CCACCATCAT CCGCATCATG 
AGAGTGCTGC GCATTGCCCG AGTGCTGAAG CTGCTGAAGA TGGCTGTGGG CATGCGGGCG 
CTGCTGGACA CAGTCATGCA GGCCCTGCCT CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
TTGTTGTTTT TCATCTTTGC AGCTCTGGGT GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACGCACC CCTGCGAGGG CCTGGGCCGG CATGCCACCT TTCGGAACTT TGGCATGGCC 
TTCCTGACCC TCTTCCGAGT GTCCACAGGT GACAACTGGA ACGGCATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACATGC TACAATACAG TCATCTCCCC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTGATCGC TGTGCTGATG 
AAGCACCTGG AGGAAAGCAA CAAGGAGGCC AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG 
GAGCTGGAGA TGAAGACGCT CAGCCCACAG CCCCACTCGC CACTGGGCAG CCCCTTCCTC 
TGGCCGGGGG TCGAGGGCCC CGACAGCCCC GATAGCCCCA AGCTCAGGGC CCCGCACACC 
GCGGCCCACA CGGGAGCAGC CTCGCGCTTC TCCCTGGAAC ACCCCACGAT GGAGCCCCAC 
CCCGAGGAGA TGCCCCTTGC ACTGGGACTG GACCTGCTGA CTGTGAGGAA GTCTGGGGTC 
AGCCGGACAC ACTCCCTGCC CAATGACAGC TACATGTTCC GGGACGGAGG CACCGCTGAG 
GGGTCCCTAG GATGCAGAAG CTGGGGGCTC CCCAAAGCTC AGTCAGGCTC TGTCTTATCT 
GTCCACTCGC AGCCAGCAGA CACCAGCTAC ATCCTGCAGC TTCCCAAAGA CACACCTCAC 
CTGCTCCAGC CCCACAGCGC TCCCACCTGG GGCACCATTC CCAAACTGCC CCCACCAGGC 
CGCTCCCCCT TGGCTCAGAG GCCACTCAGG CGCCAGGCAG CAATAAGGAC TGATTCTCTG 
GACGTTCAGG GCCTGGGCAG CCGGGAAGAC CTGCTGTCCG AGGTGAGTGG GCCCTCCCCA 
CCTCTGGCCC GGGCCTCCTC CTTCTGGGGC CACTTGGGCG CCCAGATGCA GCAGCATCCC 
CACCGCCAGA GCAAGATCTC GAAGCACATG CCCCCGCCAG CCCCCCGCCC AGGCTCAGAA 
ACCACCTGGA GCAAAGACCC ACCCGAGACC AGAAGCAGCT TAGAGCTGGA CACGGAGCTG 
AGCTGGATTT CAGGAGACCT CCTGACCGTG GGCAGCCAGG AGGAGCCCCC GTCCCCACGG 
GACCTGAAGA AGTGTCACAG CGTGGAGGCC CAGAGCTGCC GGCGCAGGCC TGCGTCCTGG 
CTGGATGAAC AGAGGAGACA CTCCATCGCG GTCAGCTGCC TGGACGGCGG CTCCCAACCC 
AGCCTGGGCC CCGGCCCCTC TAGCCTTGGC GGCCAGCCTC TTGGGGGACC TGGAAGCCGG 
CCCAAGAAAA AACTCAGCCC ACCTAGTATC TCCATAGACC CCCCAGAGAG TCAGGGCCCT 
CGGCCCCCAC CCAGCCCCGG CGTCTGCCTC CGGCGGAGGG CTCCGTCCAG CGACTCCAGG 
GACCCCTCGG CCTCCGGCCC CCCTGACAGC ATGGCTGCCT CGCCCCCCCC AAAGAAAGAC 
GTGCTGAGTC TCTCTGGTTT AGCCTCTGAC CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624660.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624717.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X27, mRNA.

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CloneID	OCe11931
Clone ID Related Accession (Same CDS sequence)	XM_005624660.2 , XM_005624660.3
Accession Version	XM_005624660.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X27
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624660.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGATC TAATGCTGGA CGATGTAATT 
GCTTCCGGCA GCTCATCCAG CGCGGCGCCA GAGGCCCAGT GTAAACCCTA CTACTCAGAC 
TACTCCCGAT TCCGGCTCCT CGTCCACCAC TTGTGTACCA GCCACTATCT GGACCTCTTC 
ATCACGGGTG TCATTGGTCT GAATGTGGTC ACCATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ACGAGGCCTT GAAGATCTGC AACTATATCT TCACCGTCAT CTTTGTCTTG 
GAGTCAGTTT TTAAACTCGT GGCCTTTGGC TTCCGTCGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATCGT GCTGCTGTCC ATCATGGGCA TCACGCTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGAGT GCTGCGCATT 
GCCCGAGTGC TGAAGCTGCT GAAGATGGCT GTGGGCATGC GGGCGCTGCT GGACACAGTC 
ATGCAGGCCC TGCCTCAGGT GGGGAACCTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGTGTGGA GCTCTTTGGA GACCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCCT GACCCTCTTC 
CGAGTGTCCA CAGGTGACAA CTGGAACGGC ATTATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CATGCTACAA TACAGTCATC TCCCCCATCT ACTTCGTGTC CTTCGTGCTG 
ACGGCCCAGT TTGTGCTGGT CAACGTGGTG ATCGCTGTGC TGATGAAGCA CCTGGAGGAA 
AGCAACAAGG AGGCCAAAGA GGAGGCCGAG CTGGAGGCTG AGCTGGAGCT GGAGATGAAG 
ACGCTCAGCC CACAGCCCCA CTCGCCACTG GGCAGCCCCT TCCTCTGGCC GGGGGTCGAG 
GGCCCCGACA GCCCCGATAG CCCCAAGCTC AGGGCCCCGC ACACCGCGGC CCACACGGGA 
GCAGCCTCGC GCTTCTCCCT GGAACACCCC ACGATGGAGC CCCACCCCGA GGAGATGCCC 
CTTGCACTGG GACTGGACCT GCTGACTGTG AGGAAGTCTG GGGTCAGCCG GACACACTCC 
CTGCCCAATG ACAGCTACAT GTTCCGGGAC GGAGGCACCG CTGAGGGGTC CCTAGGATGC 
AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA GGCTCTGTCT TATCTGTCCA CTCGCAGCCA 
GCAGACACCA GCTACATCCT GCAGCTTCCC AAAGACACAC CTCACCTGCT CCAGCCCCAC 
AGCGCTCCCA CCTGGGGCAC CATTCCCAAA CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT 
CAGAGGCCAC TCAGGCGCCA GGCAGCAATA AGGACTGATT CTCTGGACGT TCAGGGCCTG 
GGCAGCCGGG AAGACCTGCT GTCCGAGGTG AGTGGGCCCT CCCCACCTCT GGCCCGGGCC 
TCCTCCTTCT GGGGCCACTT GGGCGCCCAG ATGCAGCAGC ATCCCCACCG CCAGAGCAAG 
ATCTCGAAGC ACATGCCCCC GCCAGCCCCC CGCCCAGGCT CAGAAACCAC CTGGAGCAAA 
GACCCACCCG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGA CCGTGGGCAG CCAGGAGGAG CCCCCGTCCC CACGGGACCT GAAGAAGTGT 
CACAGCGTGG AGGCCCAGAG CTGCCGGCGC AGGCCTGCGT CCTGGCTGGA TGAACAGAGG 
AGACACTCCA TCGCGGTCAG CTGCCTGGAC GGCGGCTCCC AACCCAGCCT GGGCCCCGGC 
CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG GGACCTGGAA GCCGGCCCAA GAAAAAACTC 
AGCCCACCTA GTATCTCCAT AGACCCCCCA GAGAGTCAGG GCCCTCGGCC CCCACCCAGC 
CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG TCCAGCGACT CCAGGGACCC CTCGGCCTCC 
GGCCCCCCTG ACAGCATGGC TGCCTCGCCC CCCCCAAAGA AAGACGTGCT GAGTCTCTCT 
GGTTTAGCCT CTGACCCAGC AGACCTGGAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624717.1
CDS	527..7342
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624660.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624660.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGATC TAATGCTGGA CGATGTAATT 
GCTTCCGGCA GCTCATCCAG CGCGGCGCCA GAGGCCCAGT GTAAACCCTA CTACTCAGAC 
TACTCCCGAT TCCGGCTCCT CGTCCACCAC TTGTGTACCA GCCACTATCT GGACCTCTTC 
ATCACGGGTG TCATTGGTCT GAATGTGGTC ACCATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ACGAGGCCTT GAAGATCTGC AACTATATCT TCACCGTCAT CTTTGTCTTG 
GAGTCAGTTT TTAAACTCGT GGCCTTTGGC TTCCGTCGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATCGT GCTGCTGTCC ATCATGGGCA TCACGCTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGAGT GCTGCGCATT 
GCCCGAGTGC TGAAGCTGCT GAAGATGGCT GTGGGCATGC GGGCGCTGCT GGACACAGTC 
ATGCAGGCCC TGCCTCAGGT GGGGAACCTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGTGTGGA GCTCTTTGGA GACCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCCT GACCCTCTTC 
CGAGTGTCCA CAGGTGACAA CTGGAACGGC ATTATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CATGCTACAA TACAGTCATC TCCCCCATCT ACTTCGTGTC CTTCGTGCTG 
ACGGCCCAGT TTGTGCTGGT CAACGTGGTG ATCGCTGTGC TGATGAAGCA CCTGGAGGAA 
AGCAACAAGG AGGCCAAAGA GGAGGCCGAG CTGGAGGCTG AGCTGGAGCT GGAGATGAAG 
ACGCTCAGCC CACAGCCCCA CTCGCCACTG GGCAGCCCCT TCCTCTGGCC GGGGGTCGAG 
GGCCCCGACA GCCCCGATAG CCCCAAGCTC AGGGCCCCGC ACACCGCGGC CCACACGGGA 
GCAGCCTCGC GCTTCTCCCT GGAACACCCC ACGATGGAGC CCCACCCCGA GGAGATGCCC 
CTTGCACTGG GACTGGACCT GCTGACTGTG AGGAAGTCTG GGGTCAGCCG GACACACTCC 
CTGCCCAATG ACAGCTACAT GTTCCGGGAC GGAGGCACCG CTGAGGGGTC CCTAGGATGC 
AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA GGCTCTGTCT TATCTGTCCA CTCGCAGCCA 
GCAGACACCA GCTACATCCT GCAGCTTCCC AAAGACACAC CTCACCTGCT CCAGCCCCAC 
AGCGCTCCCA CCTGGGGCAC CATTCCCAAA CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT 
CAGAGGCCAC TCAGGCGCCA GGCAGCAATA AGGACTGATT CTCTGGACGT TCAGGGCCTG 
GGCAGCCGGG AAGACCTGCT GTCCGAGGTG AGTGGGCCCT CCCCACCTCT GGCCCGGGCC 
TCCTCCTTCT GGGGCCACTT GGGCGCCCAG ATGCAGCAGC ATCCCCACCG CCAGAGCAAG 
ATCTCGAAGC ACATGCCCCC GCCAGCCCCC CGCCCAGGCT CAGAAACCAC CTGGAGCAAA 
GACCCACCCG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGA CCGTGGGCAG CCAGGAGGAG CCCCCGTCCC CACGGGACCT GAAGAAGTGT 
CACAGCGTGG AGGCCCAGAG CTGCCGGCGC AGGCCTGCGT CCTGGCTGGA TGAACAGAGG 
AGACACTCCA TCGCGGTCAG CTGCCTGGAC GGCGGCTCCC AACCCAGCCT GGGCCCCGGC 
CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG GGACCTGGAA GCCGGCCCAA GAAAAAACTC 
AGCCCACCTA GTATCTCCAT AGACCCCCCA GAGAGTCAGG GCCCTCGGCC CCCACCCAGC 
CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG TCCAGCGACT CCAGGGACCC CTCGGCCTCC 
GGCCCCCCTG ACAGCATGGC TGCCTCGCCC CCCCCAAAGA AAGACGTGCT GAGTCTCTCT 
GGTTTAGCCT CTGACCCAGC AGACCTGGAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624660.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624717.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X23, mRNA.

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CloneID	OCe11931
Clone ID Related Accession (Same CDS sequence)	XM_005624660.2 , XM_005624660.3
Accession Version	XM_005624660.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624660.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGATC TAATGCTGGA CGATGTAATT 
GCTTCCGGCA GCTCATCCAG CGCGGCGCCA GAGGCCCAGT GTAAACCCTA CTACTCAGAC 
TACTCCCGAT TCCGGCTCCT CGTCCACCAC TTGTGTACCA GCCACTATCT GGACCTCTTC 
ATCACGGGTG TCATTGGTCT GAATGTGGTC ACCATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ACGAGGCCTT GAAGATCTGC AACTATATCT TCACCGTCAT CTTTGTCTTG 
GAGTCAGTTT TTAAACTCGT GGCCTTTGGC TTCCGTCGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATCGT GCTGCTGTCC ATCATGGGCA TCACGCTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGAGT GCTGCGCATT 
GCCCGAGTGC TGAAGCTGCT GAAGATGGCT GTGGGCATGC GGGCGCTGCT GGACACAGTC 
ATGCAGGCCC TGCCTCAGGT GGGGAACCTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGTGTGGA GCTCTTTGGA GACCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCCT GACCCTCTTC 
CGAGTGTCCA CAGGTGACAA CTGGAACGGC ATTATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CATGCTACAA TACAGTCATC TCCCCCATCT ACTTCGTGTC CTTCGTGCTG 
ACGGCCCAGT TTGTGCTGGT CAACGTGGTG ATCGCTGTGC TGATGAAGCA CCTGGAGGAA 
AGCAACAAGG AGGCCAAAGA GGAGGCCGAG CTGGAGGCTG AGCTGGAGCT GGAGATGAAG 
ACGCTCAGCC CACAGCCCCA CTCGCCACTG GGCAGCCCCT TCCTCTGGCC GGGGGTCGAG 
GGCCCCGACA GCCCCGATAG CCCCAAGCTC AGGGCCCCGC ACACCGCGGC CCACACGGGA 
GCAGCCTCGC GCTTCTCCCT GGAACACCCC ACGATGGAGC CCCACCCCGA GGAGATGCCC 
CTTGCACTGG GACTGGACCT GCTGACTGTG AGGAAGTCTG GGGTCAGCCG GACACACTCC 
CTGCCCAATG ACAGCTACAT GTTCCGGGAC GGAGGCACCG CTGAGGGGTC CCTAGGATGC 
AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA GGCTCTGTCT TATCTGTCCA CTCGCAGCCA 
GCAGACACCA GCTACATCCT GCAGCTTCCC AAAGACACAC CTCACCTGCT CCAGCCCCAC 
AGCGCTCCCA CCTGGGGCAC CATTCCCAAA CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT 
CAGAGGCCAC TCAGGCGCCA GGCAGCAATA AGGACTGATT CTCTGGACGT TCAGGGCCTG 
GGCAGCCGGG AAGACCTGCT GTCCGAGGTG AGTGGGCCCT CCCCACCTCT GGCCCGGGCC 
TCCTCCTTCT GGGGCCACTT GGGCGCCCAG ATGCAGCAGC ATCCCCACCG CCAGAGCAAG 
ATCTCGAAGC ACATGCCCCC GCCAGCCCCC CGCCCAGGCT CAGAAACCAC CTGGAGCAAA 
GACCCACCCG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGA CCGTGGGCAG CCAGGAGGAG CCCCCGTCCC CACGGGACCT GAAGAAGTGT 
CACAGCGTGG AGGCCCAGAG CTGCCGGCGC AGGCCTGCGT CCTGGCTGGA TGAACAGAGG 
AGACACTCCA TCGCGGTCAG CTGCCTGGAC GGCGGCTCCC AACCCAGCCT GGGCCCCGGC 
CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG GGACCTGGAA GCCGGCCCAA GAAAAAACTC 
AGCCCACCTA GTATCTCCAT AGACCCCCCA GAGAGTCAGG GCCCTCGGCC CCCACCCAGC 
CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG TCCAGCGACT CCAGGGACCC CTCGGCCTCC 
GGCCCCCCTG ACAGCATGGC TGCCTCGCCC CCCCCAAAGA AAGACGTGCT GAGTCTCTCT 
GGTTTAGCCT CTGACCCAGC AGACCTGGAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624717.1
CDS	504..7319
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624660.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624660.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGATC TAATGCTGGA CGATGTAATT 
GCTTCCGGCA GCTCATCCAG CGCGGCGCCA GAGGCCCAGT GTAAACCCTA CTACTCAGAC 
TACTCCCGAT TCCGGCTCCT CGTCCACCAC TTGTGTACCA GCCACTATCT GGACCTCTTC 
ATCACGGGTG TCATTGGTCT GAATGTGGTC ACCATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ACGAGGCCTT GAAGATCTGC AACTATATCT TCACCGTCAT CTTTGTCTTG 
GAGTCAGTTT TTAAACTCGT GGCCTTTGGC TTCCGTCGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATCGT GCTGCTGTCC ATCATGGGCA TCACGCTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGAGT GCTGCGCATT 
GCCCGAGTGC TGAAGCTGCT GAAGATGGCT GTGGGCATGC GGGCGCTGCT GGACACAGTC 
ATGCAGGCCC TGCCTCAGGT GGGGAACCTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGTGTGGA GCTCTTTGGA GACCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCCT GACCCTCTTC 
CGAGTGTCCA CAGGTGACAA CTGGAACGGC ATTATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CATGCTACAA TACAGTCATC TCCCCCATCT ACTTCGTGTC CTTCGTGCTG 
ACGGCCCAGT TTGTGCTGGT CAACGTGGTG ATCGCTGTGC TGATGAAGCA CCTGGAGGAA 
AGCAACAAGG AGGCCAAAGA GGAGGCCGAG CTGGAGGCTG AGCTGGAGCT GGAGATGAAG 
ACGCTCAGCC CACAGCCCCA CTCGCCACTG GGCAGCCCCT TCCTCTGGCC GGGGGTCGAG 
GGCCCCGACA GCCCCGATAG CCCCAAGCTC AGGGCCCCGC ACACCGCGGC CCACACGGGA 
GCAGCCTCGC GCTTCTCCCT GGAACACCCC ACGATGGAGC CCCACCCCGA GGAGATGCCC 
CTTGCACTGG GACTGGACCT GCTGACTGTG AGGAAGTCTG GGGTCAGCCG GACACACTCC 
CTGCCCAATG ACAGCTACAT GTTCCGGGAC GGAGGCACCG CTGAGGGGTC CCTAGGATGC 
AGAAGCTGGG GGCTCCCCAA AGCTCAGTCA GGCTCTGTCT TATCTGTCCA CTCGCAGCCA 
GCAGACACCA GCTACATCCT GCAGCTTCCC AAAGACACAC CTCACCTGCT CCAGCCCCAC 
AGCGCTCCCA CCTGGGGCAC CATTCCCAAA CTGCCCCCAC CAGGCCGCTC CCCCTTGGCT 
CAGAGGCCAC TCAGGCGCCA GGCAGCAATA AGGACTGATT CTCTGGACGT TCAGGGCCTG 
GGCAGCCGGG AAGACCTGCT GTCCGAGGTG AGTGGGCCCT CCCCACCTCT GGCCCGGGCC 
TCCTCCTTCT GGGGCCACTT GGGCGCCCAG ATGCAGCAGC ATCCCCACCG CCAGAGCAAG 
ATCTCGAAGC ACATGCCCCC GCCAGCCCCC CGCCCAGGCT CAGAAACCAC CTGGAGCAAA 
GACCCACCCG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGA CCGTGGGCAG CCAGGAGGAG CCCCCGTCCC CACGGGACCT GAAGAAGTGT 
CACAGCGTGG AGGCCCAGAG CTGCCGGCGC AGGCCTGCGT CCTGGCTGGA TGAACAGAGG 
AGACACTCCA TCGCGGTCAG CTGCCTGGAC GGCGGCTCCC AACCCAGCCT GGGCCCCGGC 
CCCTCTAGCC TTGGCGGCCA GCCTCTTGGG GGACCTGGAA GCCGGCCCAA GAAAAAACTC 
AGCCCACCTA GTATCTCCAT AGACCCCCCA GAGAGTCAGG GCCCTCGGCC CCCACCCAGC 
CCCGGCGTCT GCCTCCGGCG GAGGGCTCCG TCCAGCGACT CCAGGGACCC CTCGGCCTCC 
GGCCCCCCTG ACAGCATGGC TGCCTCGCCC CCCCCAAAGA AAGACGTGCT GAGTCTCTCT 
GGTTTAGCCT CTGACCCAGC AGACCTGGAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860654.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865747.2 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X25, mRNA.

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CloneID	OCe11932
Clone ID Related Accession (Same CDS sequence)	XM_860654.5 , XM_860654.4
Accession Version	XM_860654.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_860654.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAAGGCC CAGTGTAAAC CCTACTACTC AGACTACTCC 
CGATTCCGGC TCCTCGTCCA CCACTTGTGT ACCAGCCACT ATCTGGACCT CTTCATCACG 
GGTGTCATTG GTCTGAATGT GGTCACCATG GCCATGGAGC ACTACCAGCA GCCCCAGATC 
CTGGACGAGG CCTTGAAGAT CTGCAACTAT ATCTTCACCG TCATCTTTGT CTTGGAGTCA 
GTTTTTAAAC TCGTGGCCTT TGGCTTCCGT CGGTTCTTCC AGGACAGGTG GAACCAGCTG 
GACCTGGCCA TCGTGCTGCT GTCCATCATG GGCATCACGC TGGAGGAGAT TGAGGTCAAT 
GCCTCGCTGC CCATCAACCC CACCATCATC CGCATCATGA GAGTGCTGCG CATTGCCCGA 
GTGCTGAAGC TGCTGAAGAT GGCTGTGGGC ATGCGGGCGC TGCTGGACAC AGTCATGCAG 
GCCCTGCCTC AGGTGGGGAA CCTGGGACTT CTCTTCATGT TGTTGTTTTT CATCTTTGCA 
GCTCTGGGTG TGGAGCTCTT TGGAGACCTG GAGTGTGATG AGACGCACCC CTGCGAGGGC 
CTGGGCCGGC ATGCCACCTT TCGGAACTTT GGCATGGCCT TCCTGACCCT CTTCCGAGTG 
TCCACAGGTG ACAACTGGAA CGGCATTATG AAGGACACCC TCCGGGACTG TGACCAGGAG 
TCCACATGCT ACAATACAGT CATCTCCCCC ATCTACTTCG TGTCCTTCGT GCTGACGGCC 
CAGTTTGTGC TGGTCAACGT GGTGATCGCT GTGCTGATGA AGCACCTGGA GGAAAGCAAC 
AAGGAGGCCA AAGAGGAGGC CGAGCTGGAG GCTGAGCTGG AGCTGGAGAT GAAGACGCTC 
AGCCCACAGC CCCACTCGCC ACTGGGCAGC CCCTTCCTCT GGCCGGGGGT CGAGGGCCCC 
GACAGCCCCG ATAGCCCCAA GCTCAGGGCC CCGCACACCG CGGCCCACAC GGGAGCAGCC 
TCGCGCTTCT CCCTGGAACA CCCCACGATG GAGCCCCACC CCGAGGAGAT GCCCCTTGCA 
CTGGGACTGG ACCTGCTGAC TGTGAGGAAG TCTGGGGTCA GCCGGACACA CTCCCTGCCC 
AATGACAGCT ACATGTTCCG GGACGGAGGC ACCGCTGAGG GGTCCCTAGG ATGCAGAAGC 
TGGGGGCTCC CCAAAGCTCA GTCAGGCTCT GTCTTATCTG TCCACTCGCA GCCAGCAGAC 
ACCAGCTACA TCCTGCAGCT TCCCAAAGAC ACACCTCACC TGCTCCAGCC CCACAGCGCT 
CCCACCTGGG GCACCATTCC CAAACTGCCC CCACCAGGCC GCTCCCCCTT GGCTCAGAGG 
CCACTCAGGC GCCAGGCAGC AATAAGGACT GATTCTCTGG ACGTTCAGGG CCTGGGCAGC 
CGGGAAGACC TGCTGTCCGA GGTGAGTGGG CCCTCCCCAC CTCTGGCCCG GGCCTCCTCC 
TTCTGGGGCC ACTTGGGCGC CCAGATGCAG CAGCATCCCC ACCGCCAGAG CAAGATCTCG 
AAGCACATGC CCCCGCCAGC CCCCCGCCCA GGCTCAGAAA CCACCTGGAG CAAAGACCCA 
CCCGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGACCGTGG GCAGCCAGGA GGAGCCCCCG TCCCCACGGG ACCTGAAGAA GTGTCACAGC 
GTGGAGGCCC AGAGCTGCCG GCGCAGGCCT GCGTCCTGGC TGGATGAACA GAGGAGACAC 
TCCATCGCGG TCAGCTGCCT GGACGGCGGC TCCCAACCCA GCCTGGGCCC CGGCCCCTCT 
AGCCTTGGCG GCCAGCCTCT TGGGGGACCT GGAAGCCGGC CCAAGAAAAA ACTCAGCCCA 
CCTAGTATCT CCATAGACCC CCCAGAGAGT CAGGGCCCTC GGCCCCCACC CAGCCCCGGC 
GTCTGCCTCC GGCGGAGGGC TCCGTCCAGC GACTCCAGGG ACCCCTCGGC CTCCGGCCCC 
CCTGACAGCA TGGCTGCCTC GCCCCCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
GCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865747.2
CDS	504..7313
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860654.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860654.5

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAAGGCC CAGTGTAAAC CCTACTACTC AGACTACTCC 
CGATTCCGGC TCCTCGTCCA CCACTTGTGT ACCAGCCACT ATCTGGACCT CTTCATCACG 
GGTGTCATTG GTCTGAATGT GGTCACCATG GCCATGGAGC ACTACCAGCA GCCCCAGATC 
CTGGACGAGG CCTTGAAGAT CTGCAACTAT ATCTTCACCG TCATCTTTGT CTTGGAGTCA 
GTTTTTAAAC TCGTGGCCTT TGGCTTCCGT CGGTTCTTCC AGGACAGGTG GAACCAGCTG 
GACCTGGCCA TCGTGCTGCT GTCCATCATG GGCATCACGC TGGAGGAGAT TGAGGTCAAT 
GCCTCGCTGC CCATCAACCC CACCATCATC CGCATCATGA GAGTGCTGCG CATTGCCCGA 
GTGCTGAAGC TGCTGAAGAT GGCTGTGGGC ATGCGGGCGC TGCTGGACAC AGTCATGCAG 
GCCCTGCCTC AGGTGGGGAA CCTGGGACTT CTCTTCATGT TGTTGTTTTT CATCTTTGCA 
GCTCTGGGTG TGGAGCTCTT TGGAGACCTG GAGTGTGATG AGACGCACCC CTGCGAGGGC 
CTGGGCCGGC ATGCCACCTT TCGGAACTTT GGCATGGCCT TCCTGACCCT CTTCCGAGTG 
TCCACAGGTG ACAACTGGAA CGGCATTATG AAGGACACCC TCCGGGACTG TGACCAGGAG 
TCCACATGCT ACAATACAGT CATCTCCCCC ATCTACTTCG TGTCCTTCGT GCTGACGGCC 
CAGTTTGTGC TGGTCAACGT GGTGATCGCT GTGCTGATGA AGCACCTGGA GGAAAGCAAC 
AAGGAGGCCA AAGAGGAGGC CGAGCTGGAG GCTGAGCTGG AGCTGGAGAT GAAGACGCTC 
AGCCCACAGC CCCACTCGCC ACTGGGCAGC CCCTTCCTCT GGCCGGGGGT CGAGGGCCCC 
GACAGCCCCG ATAGCCCCAA GCTCAGGGCC CCGCACACCG CGGCCCACAC GGGAGCAGCC 
TCGCGCTTCT CCCTGGAACA CCCCACGATG GAGCCCCACC CCGAGGAGAT GCCCCTTGCA 
CTGGGACTGG ACCTGCTGAC TGTGAGGAAG TCTGGGGTCA GCCGGACACA CTCCCTGCCC 
AATGACAGCT ACATGTTCCG GGACGGAGGC ACCGCTGAGG GGTCCCTAGG ATGCAGAAGC 
TGGGGGCTCC CCAAAGCTCA GTCAGGCTCT GTCTTATCTG TCCACTCGCA GCCAGCAGAC 
ACCAGCTACA TCCTGCAGCT TCCCAAAGAC ACACCTCACC TGCTCCAGCC CCACAGCGCT 
CCCACCTGGG GCACCATTCC CAAACTGCCC CCACCAGGCC GCTCCCCCTT GGCTCAGAGG 
CCACTCAGGC GCCAGGCAGC AATAAGGACT GATTCTCTGG ACGTTCAGGG CCTGGGCAGC 
CGGGAAGACC TGCTGTCCGA GGTGAGTGGG CCCTCCCCAC CTCTGGCCCG GGCCTCCTCC 
TTCTGGGGCC ACTTGGGCGC CCAGATGCAG CAGCATCCCC ACCGCCAGAG CAAGATCTCG 
AAGCACATGC CCCCGCCAGC CCCCCGCCCA GGCTCAGAAA CCACCTGGAG CAAAGACCCA 
CCCGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGACCGTGG GCAGCCAGGA GGAGCCCCCG TCCCCACGGG ACCTGAAGAA GTGTCACAGC 
GTGGAGGCCC AGAGCTGCCG GCGCAGGCCT GCGTCCTGGC TGGATGAACA GAGGAGACAC 
TCCATCGCGG TCAGCTGCCT GGACGGCGGC TCCCAACCCA GCCTGGGCCC CGGCCCCTCT 
AGCCTTGGCG GCCAGCCTCT TGGGGGACCT GGAAGCCGGC CCAAGAAAAA ACTCAGCCCA 
CCTAGTATCT CCATAGACCC CCCAGAGAGT CAGGGCCCTC GGCCCCCACC CAGCCCCGGC 
GTCTGCCTCC GGCGGAGGGC TCCGTCCAGC GACTCCAGGG ACCCCTCGGC CTCCGGCCCC 
CCTGACAGCA TGGCTGCCTC GCCCCCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
GCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860654.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865747.2 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X29, mRNA.

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CloneID	OCe11932
Clone ID Related Accession (Same CDS sequence)	XM_860654.5 , XM_860654.4
Accession Version	XM_860654.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X29
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_860654.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAAGGCC CAGTGTAAAC CCTACTACTC AGACTACTCC 
CGATTCCGGC TCCTCGTCCA CCACTTGTGT ACCAGCCACT ATCTGGACCT CTTCATCACG 
GGTGTCATTG GTCTGAATGT GGTCACCATG GCCATGGAGC ACTACCAGCA GCCCCAGATC 
CTGGACGAGG CCTTGAAGAT CTGCAACTAT ATCTTCACCG TCATCTTTGT CTTGGAGTCA 
GTTTTTAAAC TCGTGGCCTT TGGCTTCCGT CGGTTCTTCC AGGACAGGTG GAACCAGCTG 
GACCTGGCCA TCGTGCTGCT GTCCATCATG GGCATCACGC TGGAGGAGAT TGAGGTCAAT 
GCCTCGCTGC CCATCAACCC CACCATCATC CGCATCATGA GAGTGCTGCG CATTGCCCGA 
GTGCTGAAGC TGCTGAAGAT GGCTGTGGGC ATGCGGGCGC TGCTGGACAC AGTCATGCAG 
GCCCTGCCTC AGGTGGGGAA CCTGGGACTT CTCTTCATGT TGTTGTTTTT CATCTTTGCA 
GCTCTGGGTG TGGAGCTCTT TGGAGACCTG GAGTGTGATG AGACGCACCC CTGCGAGGGC 
CTGGGCCGGC ATGCCACCTT TCGGAACTTT GGCATGGCCT TCCTGACCCT CTTCCGAGTG 
TCCACAGGTG ACAACTGGAA CGGCATTATG AAGGACACCC TCCGGGACTG TGACCAGGAG 
TCCACATGCT ACAATACAGT CATCTCCCCC ATCTACTTCG TGTCCTTCGT GCTGACGGCC 
CAGTTTGTGC TGGTCAACGT GGTGATCGCT GTGCTGATGA AGCACCTGGA GGAAAGCAAC 
AAGGAGGCCA AAGAGGAGGC CGAGCTGGAG GCTGAGCTGG AGCTGGAGAT GAAGACGCTC 
AGCCCACAGC CCCACTCGCC ACTGGGCAGC CCCTTCCTCT GGCCGGGGGT CGAGGGCCCC 
GACAGCCCCG ATAGCCCCAA GCTCAGGGCC CCGCACACCG CGGCCCACAC GGGAGCAGCC 
TCGCGCTTCT CCCTGGAACA CCCCACGATG GAGCCCCACC CCGAGGAGAT GCCCCTTGCA 
CTGGGACTGG ACCTGCTGAC TGTGAGGAAG TCTGGGGTCA GCCGGACACA CTCCCTGCCC 
AATGACAGCT ACATGTTCCG GGACGGAGGC ACCGCTGAGG GGTCCCTAGG ATGCAGAAGC 
TGGGGGCTCC CCAAAGCTCA GTCAGGCTCT GTCTTATCTG TCCACTCGCA GCCAGCAGAC 
ACCAGCTACA TCCTGCAGCT TCCCAAAGAC ACACCTCACC TGCTCCAGCC CCACAGCGCT 
CCCACCTGGG GCACCATTCC CAAACTGCCC CCACCAGGCC GCTCCCCCTT GGCTCAGAGG 
CCACTCAGGC GCCAGGCAGC AATAAGGACT GATTCTCTGG ACGTTCAGGG CCTGGGCAGC 
CGGGAAGACC TGCTGTCCGA GGTGAGTGGG CCCTCCCCAC CTCTGGCCCG GGCCTCCTCC 
TTCTGGGGCC ACTTGGGCGC CCAGATGCAG CAGCATCCCC ACCGCCAGAG CAAGATCTCG 
AAGCACATGC CCCCGCCAGC CCCCCGCCCA GGCTCAGAAA CCACCTGGAG CAAAGACCCA 
CCCGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGACCGTGG GCAGCCAGGA GGAGCCCCCG TCCCCACGGG ACCTGAAGAA GTGTCACAGC 
GTGGAGGCCC AGAGCTGCCG GCGCAGGCCT GCGTCCTGGC TGGATGAACA GAGGAGACAC 
TCCATCGCGG TCAGCTGCCT GGACGGCGGC TCCCAACCCA GCCTGGGCCC CGGCCCCTCT 
AGCCTTGGCG GCCAGCCTCT TGGGGGACCT GGAAGCCGGC CCAAGAAAAA ACTCAGCCCA 
CCTAGTATCT CCATAGACCC CCCAGAGAGT CAGGGCCCTC GGCCCCCACC CAGCCCCGGC 
GTCTGCCTCC GGCGGAGGGC TCCGTCCAGC GACTCCAGGG ACCCCTCGGC CTCCGGCCCC 
CCTGACAGCA TGGCTGCCTC GCCCCCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
GCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865747.2
CDS	526..7335
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860654.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860654.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAAGGCC CAGTGTAAAC CCTACTACTC AGACTACTCC 
CGATTCCGGC TCCTCGTCCA CCACTTGTGT ACCAGCCACT ATCTGGACCT CTTCATCACG 
GGTGTCATTG GTCTGAATGT GGTCACCATG GCCATGGAGC ACTACCAGCA GCCCCAGATC 
CTGGACGAGG CCTTGAAGAT CTGCAACTAT ATCTTCACCG TCATCTTTGT CTTGGAGTCA 
GTTTTTAAAC TCGTGGCCTT TGGCTTCCGT CGGTTCTTCC AGGACAGGTG GAACCAGCTG 
GACCTGGCCA TCGTGCTGCT GTCCATCATG GGCATCACGC TGGAGGAGAT TGAGGTCAAT 
GCCTCGCTGC CCATCAACCC CACCATCATC CGCATCATGA GAGTGCTGCG CATTGCCCGA 
GTGCTGAAGC TGCTGAAGAT GGCTGTGGGC ATGCGGGCGC TGCTGGACAC AGTCATGCAG 
GCCCTGCCTC AGGTGGGGAA CCTGGGACTT CTCTTCATGT TGTTGTTTTT CATCTTTGCA 
GCTCTGGGTG TGGAGCTCTT TGGAGACCTG GAGTGTGATG AGACGCACCC CTGCGAGGGC 
CTGGGCCGGC ATGCCACCTT TCGGAACTTT GGCATGGCCT TCCTGACCCT CTTCCGAGTG 
TCCACAGGTG ACAACTGGAA CGGCATTATG AAGGACACCC TCCGGGACTG TGACCAGGAG 
TCCACATGCT ACAATACAGT CATCTCCCCC ATCTACTTCG TGTCCTTCGT GCTGACGGCC 
CAGTTTGTGC TGGTCAACGT GGTGATCGCT GTGCTGATGA AGCACCTGGA GGAAAGCAAC 
AAGGAGGCCA AAGAGGAGGC CGAGCTGGAG GCTGAGCTGG AGCTGGAGAT GAAGACGCTC 
AGCCCACAGC CCCACTCGCC ACTGGGCAGC CCCTTCCTCT GGCCGGGGGT CGAGGGCCCC 
GACAGCCCCG ATAGCCCCAA GCTCAGGGCC CCGCACACCG CGGCCCACAC GGGAGCAGCC 
TCGCGCTTCT CCCTGGAACA CCCCACGATG GAGCCCCACC CCGAGGAGAT GCCCCTTGCA 
CTGGGACTGG ACCTGCTGAC TGTGAGGAAG TCTGGGGTCA GCCGGACACA CTCCCTGCCC 
AATGACAGCT ACATGTTCCG GGACGGAGGC ACCGCTGAGG GGTCCCTAGG ATGCAGAAGC 
TGGGGGCTCC CCAAAGCTCA GTCAGGCTCT GTCTTATCTG TCCACTCGCA GCCAGCAGAC 
ACCAGCTACA TCCTGCAGCT TCCCAAAGAC ACACCTCACC TGCTCCAGCC CCACAGCGCT 
CCCACCTGGG GCACCATTCC CAAACTGCCC CCACCAGGCC GCTCCCCCTT GGCTCAGAGG 
CCACTCAGGC GCCAGGCAGC AATAAGGACT GATTCTCTGG ACGTTCAGGG CCTGGGCAGC 
CGGGAAGACC TGCTGTCCGA GGTGAGTGGG CCCTCCCCAC CTCTGGCCCG GGCCTCCTCC 
TTCTGGGGCC ACTTGGGCGC CCAGATGCAG CAGCATCCCC ACCGCCAGAG CAAGATCTCG 
AAGCACATGC CCCCGCCAGC CCCCCGCCCA GGCTCAGAAA CCACCTGGAG CAAAGACCCA 
CCCGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGACCGTGG GCAGCCAGGA GGAGCCCCCG TCCCCACGGG ACCTGAAGAA GTGTCACAGC 
GTGGAGGCCC AGAGCTGCCG GCGCAGGCCT GCGTCCTGGC TGGATGAACA GAGGAGACAC 
TCCATCGCGG TCAGCTGCCT GGACGGCGGC TCCCAACCCA GCCTGGGCCC CGGCCCCTCT 
AGCCTTGGCG GCCAGCCTCT TGGGGGACCT GGAAGCCGGC CCAAGAAAAA ACTCAGCCCA 
CCTAGTATCT CCATAGACCC CCCAGAGAGT CAGGGCCCTC GGCCCCCACC CAGCCCCGGC 
GTCTGCCTCC GGCGGAGGGC TCCGTCCAGC GACTCCAGGG ACCCCTCGGC CTCCGGCCCC 
CCTGACAGCA TGGCTGCCTC GCCCCCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
GCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860594.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865687.2 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X26, mRNA.

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CloneID	OCe11933
Clone ID Related Accession (Same CDS sequence)	XM_860594.5 , XM_860594.4
Accession Version	XM_860594.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_860594.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAATCT AATGCTGGAC GATGTAATTG CTTCCGGCAG CTCATCCAGC 
GCGGCGCCAG AGGCCCAGTG TAAACCCTAC TACTCAGACT ACTCCCGATT CCGGCTCCTC 
GTCCACCACT TGTGTACCAG CCACTATCTG GACCTCTTCA TCACGGGTGT CATTGGTCTG 
AATGTGGTCA CCATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA CGAGGCCTTG 
AAGATCTGCA ACTATATCTT CACCGTCATC TTTGTCTTGG AGTCAGTTTT TAAACTCGTG 
GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATCGTG 
CTGCTGTCCA TCATGGGCAT CACGCTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGAGTG CTGCGCATTG CCCGAGTGCT GAAGCTGCTG 
AAGATGGCTG TGGGCATGCG GGCGCTGCTG GACACAGTCA TGCAGGCCCT GCCTCAGGTG 
GGGAACCTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGTGTGGAG 
CTCTTTGGAG ACCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC 
ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG ACCCTCTTCC GAGTGTCCAC AGGTGACAAC 
TGGAACGGCA TTATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC ATGCTACAAT 
ACAGTCATCT CCCCCATCTA CTTCGTGTCC TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC 
AACGTGGTGA TCGCTGTGCT GATGAAGCAC CTGGAGGAAA GCAACAAGGA GGCCAAAGAG 
GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG GAGATGAAGA CGCTCAGCCC ACAGCCCCAC 
TCGCCACTGG GCAGCCCCTT CCTCTGGCCG GGGGTCGAGG GCCCCGACAG CCCCGATAGC 
CCCAAGCTCA GGGCCCCGCA CACCGCGGCC CACACGGGAG CAGCCTCGCG CTTCTCCCTG 
GAACACCCCA CGATGGAGCC CCACCCCGAG GAGATGCCCC TTGCACTGGG ACTGGACCTG 
CTGACTGTGA GGAAGTCTGG GGTCAGCCGG ACACACTCCC TGCCCAATGA CAGCTACATG 
TTCCGGGACG GAGGCACCGC TGAGGGGTCC CTAGGATGCA GAAGCTGGGG GCTCCCCAAA 
GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC TCGCAGCCAG CAGACACCAG CTACATCCTG 
CAGCTTCCCA AAGACACACC TCACCTGCTC CAGCCCCACA GCGCTCCCAC CTGGGGCACC 
ATTCCCAAAC TGCCCCCACC AGGCCGCTCC CCCTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAA GGACTGATTC TCTGGACGTT CAGGGCCTGG GCAGCCGGGA AGACCTGCTG 
TCCGAGGTGA GTGGGCCCTC CCCACCTCTG GCCCGGGCCT CCTCCTTCTG GGGCCACTTG 
GGCGCCCAGA TGCAGCAGCA TCCCCACCGC CAGAGCAAGA TCTCGAAGCA CATGCCCCCG 
CCAGCCCCCC GCCCAGGCTC AGAAACCACC TGGAGCAAAG ACCCACCCGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC 
CAGGAGGAGC CCCCGTCCCC ACGGGACCTG AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT GAACAGAGGA GACACTCCAT CGCGGTCAGC 
TGCCTGGACG GCGGCTCCCA ACCCAGCCTG GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG 
CCTCTTGGGG GACCTGGAAG CCGGCCCAAG AAAAAACTCA GCCCACCTAG TATCTCCATA 
GACCCCCCAG AGAGTCAGGG CCCTCGGCCC CCACCCAGCC CCGGCGTCTG CCTCCGGCGG 
AGGGCTCCGT CCAGCGACTC CAGGGACCCC TCGGCCTCCG GCCCCCCTGA CAGCATGGCT 
GCCTCGCCCC CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTAGCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865687.2
CDS	504..7298
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860594.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860594.5

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAATCT AATGCTGGAC GATGTAATTG CTTCCGGCAG CTCATCCAGC 
GCGGCGCCAG AGGCCCAGTG TAAACCCTAC TACTCAGACT ACTCCCGATT CCGGCTCCTC 
GTCCACCACT TGTGTACCAG CCACTATCTG GACCTCTTCA TCACGGGTGT CATTGGTCTG 
AATGTGGTCA CCATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA CGAGGCCTTG 
AAGATCTGCA ACTATATCTT CACCGTCATC TTTGTCTTGG AGTCAGTTTT TAAACTCGTG 
GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATCGTG 
CTGCTGTCCA TCATGGGCAT CACGCTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGAGTG CTGCGCATTG CCCGAGTGCT GAAGCTGCTG 
AAGATGGCTG TGGGCATGCG GGCGCTGCTG GACACAGTCA TGCAGGCCCT GCCTCAGGTG 
GGGAACCTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGTGTGGAG 
CTCTTTGGAG ACCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC 
ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG ACCCTCTTCC GAGTGTCCAC AGGTGACAAC 
TGGAACGGCA TTATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC ATGCTACAAT 
ACAGTCATCT CCCCCATCTA CTTCGTGTCC TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC 
AACGTGGTGA TCGCTGTGCT GATGAAGCAC CTGGAGGAAA GCAACAAGGA GGCCAAAGAG 
GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG GAGATGAAGA CGCTCAGCCC ACAGCCCCAC 
TCGCCACTGG GCAGCCCCTT CCTCTGGCCG GGGGTCGAGG GCCCCGACAG CCCCGATAGC 
CCCAAGCTCA GGGCCCCGCA CACCGCGGCC CACACGGGAG CAGCCTCGCG CTTCTCCCTG 
GAACACCCCA CGATGGAGCC CCACCCCGAG GAGATGCCCC TTGCACTGGG ACTGGACCTG 
CTGACTGTGA GGAAGTCTGG GGTCAGCCGG ACACACTCCC TGCCCAATGA CAGCTACATG 
TTCCGGGACG GAGGCACCGC TGAGGGGTCC CTAGGATGCA GAAGCTGGGG GCTCCCCAAA 
GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC TCGCAGCCAG CAGACACCAG CTACATCCTG 
CAGCTTCCCA AAGACACACC TCACCTGCTC CAGCCCCACA GCGCTCCCAC CTGGGGCACC 
ATTCCCAAAC TGCCCCCACC AGGCCGCTCC CCCTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAA GGACTGATTC TCTGGACGTT CAGGGCCTGG GCAGCCGGGA AGACCTGCTG 
TCCGAGGTGA GTGGGCCCTC CCCACCTCTG GCCCGGGCCT CCTCCTTCTG GGGCCACTTG 
GGCGCCCAGA TGCAGCAGCA TCCCCACCGC CAGAGCAAGA TCTCGAAGCA CATGCCCCCG 
CCAGCCCCCC GCCCAGGCTC AGAAACCACC TGGAGCAAAG ACCCACCCGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC 
CAGGAGGAGC CCCCGTCCCC ACGGGACCTG AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT GAACAGAGGA GACACTCCAT CGCGGTCAGC 
TGCCTGGACG GCGGCTCCCA ACCCAGCCTG GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG 
CCTCTTGGGG GACCTGGAAG CCGGCCCAAG AAAAAACTCA GCCCACCTAG TATCTCCATA 
GACCCCCCAG AGAGTCAGGG CCCTCGGCCC CCACCCAGCC CCGGCGTCTG CCTCCGGCGG 
AGGGCTCCGT CCAGCGACTC CAGGGACCCC TCGGCCTCCG GCCCCCCTGA CAGCATGGCT 
GCCTCGCCCC CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTAGCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860594.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865687.2 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X30, mRNA.

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CloneID	OCe11933
Clone ID Related Accession (Same CDS sequence)	XM_860594.5 , XM_860594.4
Accession Version	XM_860594.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X30
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_860594.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAATCT AATGCTGGAC GATGTAATTG CTTCCGGCAG CTCATCCAGC 
GCGGCGCCAG AGGCCCAGTG TAAACCCTAC TACTCAGACT ACTCCCGATT CCGGCTCCTC 
GTCCACCACT TGTGTACCAG CCACTATCTG GACCTCTTCA TCACGGGTGT CATTGGTCTG 
AATGTGGTCA CCATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA CGAGGCCTTG 
AAGATCTGCA ACTATATCTT CACCGTCATC TTTGTCTTGG AGTCAGTTTT TAAACTCGTG 
GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATCGTG 
CTGCTGTCCA TCATGGGCAT CACGCTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGAGTG CTGCGCATTG CCCGAGTGCT GAAGCTGCTG 
AAGATGGCTG TGGGCATGCG GGCGCTGCTG GACACAGTCA TGCAGGCCCT GCCTCAGGTG 
GGGAACCTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGTGTGGAG 
CTCTTTGGAG ACCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC 
ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG ACCCTCTTCC GAGTGTCCAC AGGTGACAAC 
TGGAACGGCA TTATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC ATGCTACAAT 
ACAGTCATCT CCCCCATCTA CTTCGTGTCC TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC 
AACGTGGTGA TCGCTGTGCT GATGAAGCAC CTGGAGGAAA GCAACAAGGA GGCCAAAGAG 
GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG GAGATGAAGA CGCTCAGCCC ACAGCCCCAC 
TCGCCACTGG GCAGCCCCTT CCTCTGGCCG GGGGTCGAGG GCCCCGACAG CCCCGATAGC 
CCCAAGCTCA GGGCCCCGCA CACCGCGGCC CACACGGGAG CAGCCTCGCG CTTCTCCCTG 
GAACACCCCA CGATGGAGCC CCACCCCGAG GAGATGCCCC TTGCACTGGG ACTGGACCTG 
CTGACTGTGA GGAAGTCTGG GGTCAGCCGG ACACACTCCC TGCCCAATGA CAGCTACATG 
TTCCGGGACG GAGGCACCGC TGAGGGGTCC CTAGGATGCA GAAGCTGGGG GCTCCCCAAA 
GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC TCGCAGCCAG CAGACACCAG CTACATCCTG 
CAGCTTCCCA AAGACACACC TCACCTGCTC CAGCCCCACA GCGCTCCCAC CTGGGGCACC 
ATTCCCAAAC TGCCCCCACC AGGCCGCTCC CCCTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAA GGACTGATTC TCTGGACGTT CAGGGCCTGG GCAGCCGGGA AGACCTGCTG 
TCCGAGGTGA GTGGGCCCTC CCCACCTCTG GCCCGGGCCT CCTCCTTCTG GGGCCACTTG 
GGCGCCCAGA TGCAGCAGCA TCCCCACCGC CAGAGCAAGA TCTCGAAGCA CATGCCCCCG 
CCAGCCCCCC GCCCAGGCTC AGAAACCACC TGGAGCAAAG ACCCACCCGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC 
CAGGAGGAGC CCCCGTCCCC ACGGGACCTG AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT GAACAGAGGA GACACTCCAT CGCGGTCAGC 
TGCCTGGACG GCGGCTCCCA ACCCAGCCTG GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG 
CCTCTTGGGG GACCTGGAAG CCGGCCCAAG AAAAAACTCA GCCCACCTAG TATCTCCATA 
GACCCCCCAG AGAGTCAGGG CCCTCGGCCC CCACCCAGCC CCGGCGTCTG CCTCCGGCGG 
AGGGCTCCGT CCAGCGACTC CAGGGACCCC TCGGCCTCCG GCCCCCCTGA CAGCATGGCT 
GCCTCGCCCC CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTAGCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865687.2
CDS	527..7321
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860594.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860594.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAATCT AATGCTGGAC GATGTAATTG CTTCCGGCAG CTCATCCAGC 
GCGGCGCCAG AGGCCCAGTG TAAACCCTAC TACTCAGACT ACTCCCGATT CCGGCTCCTC 
GTCCACCACT TGTGTACCAG CCACTATCTG GACCTCTTCA TCACGGGTGT CATTGGTCTG 
AATGTGGTCA CCATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA CGAGGCCTTG 
AAGATCTGCA ACTATATCTT CACCGTCATC TTTGTCTTGG AGTCAGTTTT TAAACTCGTG 
GCCTTTGGCT TCCGTCGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATCGTG 
CTGCTGTCCA TCATGGGCAT CACGCTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGAGTG CTGCGCATTG CCCGAGTGCT GAAGCTGCTG 
AAGATGGCTG TGGGCATGCG GGCGCTGCTG GACACAGTCA TGCAGGCCCT GCCTCAGGTG 
GGGAACCTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGTGTGGAG 
CTCTTTGGAG ACCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC 
ACCTTTCGGA ACTTTGGCAT GGCCTTCCTG ACCCTCTTCC GAGTGTCCAC AGGTGACAAC 
TGGAACGGCA TTATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC ATGCTACAAT 
ACAGTCATCT CCCCCATCTA CTTCGTGTCC TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC 
AACGTGGTGA TCGCTGTGCT GATGAAGCAC CTGGAGGAAA GCAACAAGGA GGCCAAAGAG 
GAGGCCGAGC TGGAGGCTGA GCTGGAGCTG GAGATGAAGA CGCTCAGCCC ACAGCCCCAC 
TCGCCACTGG GCAGCCCCTT CCTCTGGCCG GGGGTCGAGG GCCCCGACAG CCCCGATAGC 
CCCAAGCTCA GGGCCCCGCA CACCGCGGCC CACACGGGAG CAGCCTCGCG CTTCTCCCTG 
GAACACCCCA CGATGGAGCC CCACCCCGAG GAGATGCCCC TTGCACTGGG ACTGGACCTG 
CTGACTGTGA GGAAGTCTGG GGTCAGCCGG ACACACTCCC TGCCCAATGA CAGCTACATG 
TTCCGGGACG GAGGCACCGC TGAGGGGTCC CTAGGATGCA GAAGCTGGGG GCTCCCCAAA 
GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC TCGCAGCCAG CAGACACCAG CTACATCCTG 
CAGCTTCCCA AAGACACACC TCACCTGCTC CAGCCCCACA GCGCTCCCAC CTGGGGCACC 
ATTCCCAAAC TGCCCCCACC AGGCCGCTCC CCCTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAA GGACTGATTC TCTGGACGTT CAGGGCCTGG GCAGCCGGGA AGACCTGCTG 
TCCGAGGTGA GTGGGCCCTC CCCACCTCTG GCCCGGGCCT CCTCCTTCTG GGGCCACTTG 
GGCGCCCAGA TGCAGCAGCA TCCCCACCGC CAGAGCAAGA TCTCGAAGCA CATGCCCCCG 
CCAGCCCCCC GCCCAGGCTC AGAAACCACC TGGAGCAAAG ACCCACCCGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC 
CAGGAGGAGC CCCCGTCCCC ACGGGACCTG AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT GAACAGAGGA GACACTCCAT CGCGGTCAGC 
TGCCTGGACG GCGGCTCCCA ACCCAGCCTG GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG 
CCTCTTGGGG GACCTGGAAG CCGGCCCAAG AAAAAACTCA GCCCACCTAG TATCTCCATA 
GACCCCCCAG AGAGTCAGGG CCCTCGGCCC CCACCCAGCC CCGGCGTCTG CCTCCGGCGG 
AGGGCTCCGT CCAGCGACTC CAGGGACCCC TCGGCCTCCG GCCCCCCTGA CAGCATGGCT 
GCCTCGCCCC CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTAGCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860802.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865895.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X27, mRNA.

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CloneID	OCe11936
Clone ID Related Accession (Same CDS sequence)	XM_860802.5 , XM_860802.4
Accession Version	XM_860802.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_860802.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGAGG CCCAGTGTAA ACCCTACTAC 
TCAGACTACT CCCGATTCCG GCTCCTCGTC CACCACTTGT GTACCAGCCA CTATCTGGAC 
CTCTTCATCA CGGGTGTCAT TGGTCTGAAT GTGGTCACCA TGGCCATGGA GCACTACCAG 
CAGCCCCAGA TCCTGGACGA GGCCTTGAAG ATCTGCAACT ATATCTTCAC CGTCATCTTT 
GTCTTGGAGT CAGTTTTTAA ACTCGTGGCC TTTGGCTTCC GTCGGTTCTT CCAGGACAGG 
TGGAACCAGC TGGACCTGGC CATCGTGCTG CTGTCCATCA TGGGCATCAC GCTGGAGGAG 
ATTGAGGTCA ATGCCTCGCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGAGTGCTG 
CGCATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCTGTGG GCATGCGGGC GCTGCTGGAC 
ACAGTCATGC AGGCCCTGCC TCAGGTGGGG AACCTGGGAC TTCTCTTCAT GTTGTTGTTT 
TTCATCTTTG CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC 
CCCTGCGAGG GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGACC 
CTCTTCCGAG TGTCCACAGG TGACAACTGG AACGGCATTA TGAAGGACAC CCTCCGGGAC 
TGTGACCAGG AGTCCACATG CTACAATACA GTCATCTCCC CCATCTACTT CGTGTCCTTC 
GTGCTGACGG CCCAGTTTGT GCTGGTCAAC GTGGTGATCG CTGTGCTGAT GAAGCACCTG 
GAGGAAAGCA ACAAGGAGGC CAAAGAGGAG GCCGAGCTGG AGGCTGAGCT GGAGCTGGAG 
ATGAAGACGC TCAGCCCACA GCCCCACTCG CCACTGGGCA GCCCCTTCCT CTGGCCGGGG 
GTCGAGGGCC CCGACAGCCC CGATAGCCCC AAGCTCAGGG CCCCGCACAC CGCGGCCCAC 
ACGGGAGCAG CCTCGCGCTT CTCCCTGGAA CACCCCACGA TGGAGCCCCA CCCCGAGGAG 
ATGCCCCTTG CACTGGGACT GGACCTGCTG ACTGTGAGGA AGTCTGGGGT CAGCCGGACA 
CACTCCCTGC CCAATGACAG CTACATGTTC CGGGACGGAG GCACCGCTGA GGGGTCCCTA 
GGATGCAGAA GCTGGGGGCT CCCCAAAGCT CAGTCAGGCT CTGTCTTATC TGTCCACTCG 
CAGCCAGCAG ACACCAGCTA CATCCTGCAG CTTCCCAAAG ACACACCTCA CCTGCTCCAG 
CCCCACAGCG CTCCCACCTG GGGCACCATT CCCAAACTGC CCCCACCAGG CCGCTCCCCC 
TTGGCTCAGA GGCCACTCAG GCGCCAGGCA GCAATAAGGA CTGATTCTCT GGACGTTCAG 
GGCCTGGGCA GCCGGGAAGA CCTGCTGTCC GAGGTGAGTG GGCCCTCCCC ACCTCTGGCC 
CGGGCCTCCT CCTTCTGGGG CCACTTGGGC GCCCAGATGC AGCAGCATCC CCACCGCCAG 
AGCAAGATCT CGAAGCACAT GCCCCCGCCA GCCCCCCGCC CAGGCTCAGA AACCACCTGG 
AGCAAAGACC CACCCGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGACCGT GGGCAGCCAG GAGGAGCCCC CGTCCCCACG GGACCTGAAG 
AAGTGTCACA GCGTGGAGGC CCAGAGCTGC CGGCGCAGGC CTGCGTCCTG GCTGGATGAA 
CAGAGGAGAC ACTCCATCGC GGTCAGCTGC CTGGACGGCG GCTCCCAACC CAGCCTGGGC 
CCCGGCCCCT CTAGCCTTGG CGGCCAGCCT CTTGGGGGAC CTGGAAGCCG GCCCAAGAAA 
AAACTCAGCC CACCTAGTAT CTCCATAGAC CCCCCAGAGA GTCAGGGCCC TCGGCCCCCA 
CCCAGCCCCG GCGTCTGCCT CCGGCGGAGG GCTCCGTCCA GCGACTCCAG GGACCCCTCG 
GCCTCCGGCC CCCCTGACAG CATGGCTGCC TCGCCCCCCC CAAAGAAAGA CGTGCTGAGT 
CTCTCTGGTT TAGCCTCTGA CCCAGCAGAC CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865895.1
CDS	503..7264
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860802.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860802.5

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGAGG CCCAGTGTAA ACCCTACTAC 
TCAGACTACT CCCGATTCCG GCTCCTCGTC CACCACTTGT GTACCAGCCA CTATCTGGAC 
CTCTTCATCA CGGGTGTCAT TGGTCTGAAT GTGGTCACCA TGGCCATGGA GCACTACCAG 
CAGCCCCAGA TCCTGGACGA GGCCTTGAAG ATCTGCAACT ATATCTTCAC CGTCATCTTT 
GTCTTGGAGT CAGTTTTTAA ACTCGTGGCC TTTGGCTTCC GTCGGTTCTT CCAGGACAGG 
TGGAACCAGC TGGACCTGGC CATCGTGCTG CTGTCCATCA TGGGCATCAC GCTGGAGGAG 
ATTGAGGTCA ATGCCTCGCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGAGTGCTG 
CGCATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCTGTGG GCATGCGGGC GCTGCTGGAC 
ACAGTCATGC AGGCCCTGCC TCAGGTGGGG AACCTGGGAC TTCTCTTCAT GTTGTTGTTT 
TTCATCTTTG CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC 
CCCTGCGAGG GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGACC 
CTCTTCCGAG TGTCCACAGG TGACAACTGG AACGGCATTA TGAAGGACAC CCTCCGGGAC 
TGTGACCAGG AGTCCACATG CTACAATACA GTCATCTCCC CCATCTACTT CGTGTCCTTC 
GTGCTGACGG CCCAGTTTGT GCTGGTCAAC GTGGTGATCG CTGTGCTGAT GAAGCACCTG 
GAGGAAAGCA ACAAGGAGGC CAAAGAGGAG GCCGAGCTGG AGGCTGAGCT GGAGCTGGAG 
ATGAAGACGC TCAGCCCACA GCCCCACTCG CCACTGGGCA GCCCCTTCCT CTGGCCGGGG 
GTCGAGGGCC CCGACAGCCC CGATAGCCCC AAGCTCAGGG CCCCGCACAC CGCGGCCCAC 
ACGGGAGCAG CCTCGCGCTT CTCCCTGGAA CACCCCACGA TGGAGCCCCA CCCCGAGGAG 
ATGCCCCTTG CACTGGGACT GGACCTGCTG ACTGTGAGGA AGTCTGGGGT CAGCCGGACA 
CACTCCCTGC CCAATGACAG CTACATGTTC CGGGACGGAG GCACCGCTGA GGGGTCCCTA 
GGATGCAGAA GCTGGGGGCT CCCCAAAGCT CAGTCAGGCT CTGTCTTATC TGTCCACTCG 
CAGCCAGCAG ACACCAGCTA CATCCTGCAG CTTCCCAAAG ACACACCTCA CCTGCTCCAG 
CCCCACAGCG CTCCCACCTG GGGCACCATT CCCAAACTGC CCCCACCAGG CCGCTCCCCC 
TTGGCTCAGA GGCCACTCAG GCGCCAGGCA GCAATAAGGA CTGATTCTCT GGACGTTCAG 
GGCCTGGGCA GCCGGGAAGA CCTGCTGTCC GAGGTGAGTG GGCCCTCCCC ACCTCTGGCC 
CGGGCCTCCT CCTTCTGGGG CCACTTGGGC GCCCAGATGC AGCAGCATCC CCACCGCCAG 
AGCAAGATCT CGAAGCACAT GCCCCCGCCA GCCCCCCGCC CAGGCTCAGA AACCACCTGG 
AGCAAAGACC CACCCGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGACCGT GGGCAGCCAG GAGGAGCCCC CGTCCCCACG GGACCTGAAG 
AAGTGTCACA GCGTGGAGGC CCAGAGCTGC CGGCGCAGGC CTGCGTCCTG GCTGGATGAA 
CAGAGGAGAC ACTCCATCGC GGTCAGCTGC CTGGACGGCG GCTCCCAACC CAGCCTGGGC 
CCCGGCCCCT CTAGCCTTGG CGGCCAGCCT CTTGGGGGAC CTGGAAGCCG GCCCAAGAAA 
AAACTCAGCC CACCTAGTAT CTCCATAGAC CCCCCAGAGA GTCAGGGCCC TCGGCCCCCA 
CCCAGCCCCG GCGTCTGCCT CCGGCGGAGG GCTCCGTCCA GCGACTCCAG GGACCCCTCG 
GCCTCCGGCC CCCCTGACAG CATGGCTGCC TCGCCCCCCC CAAAGAAAGA CGTGCTGAGT 
CTCTCTGGTT TAGCCTCTGA CCCAGCAGAC CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860802.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865895.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X31, mRNA.

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CloneID	OCe11936
Clone ID Related Accession (Same CDS sequence)	XM_860802.5 , XM_860802.4
Accession Version	XM_860802.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X31
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_860802.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGAGG CCCAGTGTAA ACCCTACTAC 
TCAGACTACT CCCGATTCCG GCTCCTCGTC CACCACTTGT GTACCAGCCA CTATCTGGAC 
CTCTTCATCA CGGGTGTCAT TGGTCTGAAT GTGGTCACCA TGGCCATGGA GCACTACCAG 
CAGCCCCAGA TCCTGGACGA GGCCTTGAAG ATCTGCAACT ATATCTTCAC CGTCATCTTT 
GTCTTGGAGT CAGTTTTTAA ACTCGTGGCC TTTGGCTTCC GTCGGTTCTT CCAGGACAGG 
TGGAACCAGC TGGACCTGGC CATCGTGCTG CTGTCCATCA TGGGCATCAC GCTGGAGGAG 
ATTGAGGTCA ATGCCTCGCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGAGTGCTG 
CGCATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCTGTGG GCATGCGGGC GCTGCTGGAC 
ACAGTCATGC AGGCCCTGCC TCAGGTGGGG AACCTGGGAC TTCTCTTCAT GTTGTTGTTT 
TTCATCTTTG CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC 
CCCTGCGAGG GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGACC 
CTCTTCCGAG TGTCCACAGG TGACAACTGG AACGGCATTA TGAAGGACAC CCTCCGGGAC 
TGTGACCAGG AGTCCACATG CTACAATACA GTCATCTCCC CCATCTACTT CGTGTCCTTC 
GTGCTGACGG CCCAGTTTGT GCTGGTCAAC GTGGTGATCG CTGTGCTGAT GAAGCACCTG 
GAGGAAAGCA ACAAGGAGGC CAAAGAGGAG GCCGAGCTGG AGGCTGAGCT GGAGCTGGAG 
ATGAAGACGC TCAGCCCACA GCCCCACTCG CCACTGGGCA GCCCCTTCCT CTGGCCGGGG 
GTCGAGGGCC CCGACAGCCC CGATAGCCCC AAGCTCAGGG CCCCGCACAC CGCGGCCCAC 
ACGGGAGCAG CCTCGCGCTT CTCCCTGGAA CACCCCACGA TGGAGCCCCA CCCCGAGGAG 
ATGCCCCTTG CACTGGGACT GGACCTGCTG ACTGTGAGGA AGTCTGGGGT CAGCCGGACA 
CACTCCCTGC CCAATGACAG CTACATGTTC CGGGACGGAG GCACCGCTGA GGGGTCCCTA 
GGATGCAGAA GCTGGGGGCT CCCCAAAGCT CAGTCAGGCT CTGTCTTATC TGTCCACTCG 
CAGCCAGCAG ACACCAGCTA CATCCTGCAG CTTCCCAAAG ACACACCTCA CCTGCTCCAG 
CCCCACAGCG CTCCCACCTG GGGCACCATT CCCAAACTGC CCCCACCAGG CCGCTCCCCC 
TTGGCTCAGA GGCCACTCAG GCGCCAGGCA GCAATAAGGA CTGATTCTCT GGACGTTCAG 
GGCCTGGGCA GCCGGGAAGA CCTGCTGTCC GAGGTGAGTG GGCCCTCCCC ACCTCTGGCC 
CGGGCCTCCT CCTTCTGGGG CCACTTGGGC GCCCAGATGC AGCAGCATCC CCACCGCCAG 
AGCAAGATCT CGAAGCACAT GCCCCCGCCA GCCCCCCGCC CAGGCTCAGA AACCACCTGG 
AGCAAAGACC CACCCGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGACCGT GGGCAGCCAG GAGGAGCCCC CGTCCCCACG GGACCTGAAG 
AAGTGTCACA GCGTGGAGGC CCAGAGCTGC CGGCGCAGGC CTGCGTCCTG GCTGGATGAA 
CAGAGGAGAC ACTCCATCGC GGTCAGCTGC CTGGACGGCG GCTCCCAACC CAGCCTGGGC 
CCCGGCCCCT CTAGCCTTGG CGGCCAGCCT CTTGGGGGAC CTGGAAGCCG GCCCAAGAAA 
AAACTCAGCC CACCTAGTAT CTCCATAGAC CCCCCAGAGA GTCAGGGCCC TCGGCCCCCA 
CCCAGCCCCG GCGTCTGCCT CCGGCGGAGG GCTCCGTCCA GCGACTCCAG GGACCCCTCG 
GCCTCCGGCC CCCCTGACAG CATGGCTGCC TCGCCCCCCC CAAAGAAAGA CGTGCTGAGT 
CTCTCTGGTT TAGCCTCTGA CCCAGCAGAC CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865895.1
CDS	526..7287
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860802.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860802.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGAGG CCCAGTGTAA ACCCTACTAC 
TCAGACTACT CCCGATTCCG GCTCCTCGTC CACCACTTGT GTACCAGCCA CTATCTGGAC 
CTCTTCATCA CGGGTGTCAT TGGTCTGAAT GTGGTCACCA TGGCCATGGA GCACTACCAG 
CAGCCCCAGA TCCTGGACGA GGCCTTGAAG ATCTGCAACT ATATCTTCAC CGTCATCTTT 
GTCTTGGAGT CAGTTTTTAA ACTCGTGGCC TTTGGCTTCC GTCGGTTCTT CCAGGACAGG 
TGGAACCAGC TGGACCTGGC CATCGTGCTG CTGTCCATCA TGGGCATCAC GCTGGAGGAG 
ATTGAGGTCA ATGCCTCGCT GCCCATCAAC CCCACCATCA TCCGCATCAT GAGAGTGCTG 
CGCATTGCCC GAGTGCTGAA GCTGCTGAAG ATGGCTGTGG GCATGCGGGC GCTGCTGGAC 
ACAGTCATGC AGGCCCTGCC TCAGGTGGGG AACCTGGGAC TTCTCTTCAT GTTGTTGTTT 
TTCATCTTTG CAGCTCTGGG TGTGGAGCTC TTTGGAGACC TGGAGTGTGA TGAGACGCAC 
CCCTGCGAGG GCCTGGGCCG GCATGCCACC TTTCGGAACT TTGGCATGGC CTTCCTGACC 
CTCTTCCGAG TGTCCACAGG TGACAACTGG AACGGCATTA TGAAGGACAC CCTCCGGGAC 
TGTGACCAGG AGTCCACATG CTACAATACA GTCATCTCCC CCATCTACTT CGTGTCCTTC 
GTGCTGACGG CCCAGTTTGT GCTGGTCAAC GTGGTGATCG CTGTGCTGAT GAAGCACCTG 
GAGGAAAGCA ACAAGGAGGC CAAAGAGGAG GCCGAGCTGG AGGCTGAGCT GGAGCTGGAG 
ATGAAGACGC TCAGCCCACA GCCCCACTCG CCACTGGGCA GCCCCTTCCT CTGGCCGGGG 
GTCGAGGGCC CCGACAGCCC CGATAGCCCC AAGCTCAGGG CCCCGCACAC CGCGGCCCAC 
ACGGGAGCAG CCTCGCGCTT CTCCCTGGAA CACCCCACGA TGGAGCCCCA CCCCGAGGAG 
ATGCCCCTTG CACTGGGACT GGACCTGCTG ACTGTGAGGA AGTCTGGGGT CAGCCGGACA 
CACTCCCTGC CCAATGACAG CTACATGTTC CGGGACGGAG GCACCGCTGA GGGGTCCCTA 
GGATGCAGAA GCTGGGGGCT CCCCAAAGCT CAGTCAGGCT CTGTCTTATC TGTCCACTCG 
CAGCCAGCAG ACACCAGCTA CATCCTGCAG CTTCCCAAAG ACACACCTCA CCTGCTCCAG 
CCCCACAGCG CTCCCACCTG GGGCACCATT CCCAAACTGC CCCCACCAGG CCGCTCCCCC 
TTGGCTCAGA GGCCACTCAG GCGCCAGGCA GCAATAAGGA CTGATTCTCT GGACGTTCAG 
GGCCTGGGCA GCCGGGAAGA CCTGCTGTCC GAGGTGAGTG GGCCCTCCCC ACCTCTGGCC 
CGGGCCTCCT CCTTCTGGGG CCACTTGGGC GCCCAGATGC AGCAGCATCC CCACCGCCAG 
AGCAAGATCT CGAAGCACAT GCCCCCGCCA GCCCCCCGCC CAGGCTCAGA AACCACCTGG 
AGCAAAGACC CACCCGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGACCGT GGGCAGCCAG GAGGAGCCCC CGTCCCCACG GGACCTGAAG 
AAGTGTCACA GCGTGGAGGC CCAGAGCTGC CGGCGCAGGC CTGCGTCCTG GCTGGATGAA 
CAGAGGAGAC ACTCCATCGC GGTCAGCTGC CTGGACGGCG GCTCCCAACC CAGCCTGGGC 
CCCGGCCCCT CTAGCCTTGG CGGCCAGCCT CTTGGGGGAC CTGGAAGCCG GCCCAAGAAA 
AAACTCAGCC CACCTAGTAT CTCCATAGAC CCCCCAGAGA GTCAGGGCCC TCGGCCCCCA 
CCCAGCCCCG GCGTCTGCCT CCGGCGGAGG GCTCCGTCCA GCGACTCCAG GGACCCCTCG 
GCCTCCGGCC CCCCTGACAG CATGGCTGCC TCGCCCCCCC CAAAGAAAGA CGTGCTGAGT 
CTCTCTGGTT TAGCCTCTGA CCCAGCAGAC CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624663.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624720.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X32, mRNA.

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CloneID	OCe11937
Clone ID Related Accession (Same CDS sequence)	XM_005624663.2 , XM_005624663.3
Accession Version	XM_005624663.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X32
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
TTCTCAGAGA CCAAGCAGCG GGAAAGCCAG CTGATGCGAG AGCAACGTGT GCGGTTCCTG 
TCCAACGCCA GCACCCTGGC CAGCTTCTCT GAGCCGGGCA GCTGCTACGA GGAGCTGCTC 
AAGTACCTGG TGTACATCCT CCGTAAAGCG GCCCGCAGGC TGGCCCAGGT CTCCCGGGCG 
GCGGGCGTGC GGGTCGGCCT GCTGAGCAGC CCAGTGCCCC GTGGAGGCCA GGAGCCCCAG 
TCTGGCGGCA GCTGCTCTCG CTCCCACCGC CGTCCATCCG TCCACCACCT GGTGCACCAC 
CACCACCACC ATCACCACCA CTACCATCTG GGCAATGGGA CGCTGAGGGC CCCCCGGGCC 
AGCCCGGAGA TTCAGGACAA GGATGCCAGT GGGTCCCGCC GGCTCATGCT GCCACCACCC 
TCGACGCCAG CCCTCTCTGG GGGCCCTCCA GGGGGTGCAG AATCTGTGCA CAGCTTCTAC 
CATGCCGACT GCCACCTGGA GCCGGTCCGC TGCCAGGCAC CCCCTCCCAG GTCACCATCT 
GAGGCGTCGG GCAGGACTGT GGGCAGCGGG AAGGTGTACC CCACTGTGCA CACCAGCCCT 
CCACCAGAGA TGCTGAAGGA GAAAGCGCTA GTGGAGGTGG CCCCCACTTC TGGACCCCCC 
ACCCTCACCA GCCTCAACAT CCCGCCCGGG CCCTACAGCT CCATGCACAA GCTACTAGAG 
ACACAGAGCA CAGGCGCCTG CCAAAGCTCT TGCAAGATCT CCAGCCCTTG CCTGAAGGCA 
GACAGTGGAG CCGGTGGCCC AGACAGCTGC CCCTACTGTG CCCGGGCGGG GGCAGGGCAG 
GTGGAGCTCG CCGGCCACGA GATGCCTGAC TCAGACAGCG AGGCAGTTTA TGAGCTCACA 
CAAGACGTCC ACCACGGTGA TCTCCGGGAC CCCCACAGCC GGCACCGCCA GAGCCTGGGG 
CTGGACGTGG AGCCCAACTC TGTGCTGGCC TTCTGGAGGC TGATCTGTGA CACTTTCCGG 
AAGATTGTGG ACAGCAAGTA CTTTGGTCGG GGAATCATGA TTGCTATCCT GGTCAACACG 
CTCAGCATGG GCATCGAGTA CCATGAACAG CCCGAGGAGC TGACCAACGC CCTAGAAATT 
AGCAACATCG TCTTCACCAG CCTCTTTGCC CTGGAGATGC TGCTGAAGCT GCTGGTGTAC 
GGTCCCTTTG GCTACATCAA GAACCCCTAC AATATCTTCG ATGGCGTCAT TGTTGTCATC 
AGCGTGTGGG AGATCGTGGG CCAGCAGGGG GGTGGCCTGT CGGTGCTGCG GACCTTCCGC 
CTGATGCGGG TGCTGAAGCT GGTGCGCTTC CTGCCGGCAC TGCAGCGGCA GCTCGTGGTG 
CTCATGAAGA CCATGGACAA CGTGGCCACC TTCTGCATGC TACTCATGCT CTTCATCTTC 
ATCTTCAGCA TCCTGGGCAT GCATCTCTTT GGCTGCAAAT TTGCATCTGA GCGGGACGGG 
GACACGCTGC CGGACCGGAA GAATTTTGAC TCCTTGCTCT GGGCCATCGT CACCGTCTTT 
CAGATCCTGA CCCAGGAGGA CTGGAACAAG GTGCTCTACA ATGGAATGGC CTCCACATCA 
TCCTGGGCCG CCCTTTATTT CATTGCCCTC ATGACCTTTG GCAACTACGT GCTCTTTAAT 
CTGCTTGTCG CCATTCTGGT GGAGGGCTTC CAGGCGGAGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GATGGAGCCC CACCCCGAGG AGATGCCCCT TGCACTGGGA 
CTGGACCTGC TGACTGTGAG GAAGTCTGGG GTCAGCCGGA CACACTCCCT GCCCAATGAC 
AGCTACATGT TCCGGGACGG AGGCACCGCT GAGGGGTCCC TAGGATGCAG AAGCTGGGGG 
CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA TCTGTCCACT CGCAGCCAGC AGACACCAGC 
TACATCCTGC AGCTTCCCAA AGACACACCT CACCTGCTCC AGCCCCACAG CGCTCCCACC 
TGGGGCACCA TTCCCAAACT GCCCCCACCA GGCCGCTCCC CCTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATAAG GACTGATTCT CTGGACGTTC AGGGCCTGGG CAGCCGGGAA 
GACCTGCTGT CCGAGGTGAG TGGGCCCTCC CCACCTCTGG CCCGGGCCTC CTCCTTCTGG 
GGCCACTTGG GCGCCCAGAT GCAGCAGCAT CCCCACCGCC AGAGCAAGAT CTCGAAGCAC 
ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA GAAACCACCT GGAGCAAAGA CCCACCCGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGACC 
GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA CGGGACCTGA AGAAGTGTCA CAGCGTGGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC TGGCTGGATG AACAGAGGAG ACACTCCATC 
GCGGTCAGCT GCCTGGACGG CGGCTCCCAA CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT 
GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC CGGCCCAAGA AAAAACTCAG CCCACCTAGT 
ATCTCCATAG ACCCCCCAGA GAGTCAGGGC CCTCGGCCCC CACCCAGCCC CGGCGTCTGC 
CTCCGGCGGA GGGCTCCGTC CAGCGACTCC AGGGACCCCT CGGCCTCCGG CCCCCCTGAC 
AGCATGGCTG CCTCGCCCCC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTAGCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624720.1
CDS	527..7270
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624663.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624663.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
TTCTCAGAGA CCAAGCAGCG GGAAAGCCAG CTGATGCGAG AGCAACGTGT GCGGTTCCTG 
TCCAACGCCA GCACCCTGGC CAGCTTCTCT GAGCCGGGCA GCTGCTACGA GGAGCTGCTC 
AAGTACCTGG TGTACATCCT CCGTAAAGCG GCCCGCAGGC TGGCCCAGGT CTCCCGGGCG 
GCGGGCGTGC GGGTCGGCCT GCTGAGCAGC CCAGTGCCCC GTGGAGGCCA GGAGCCCCAG 
TCTGGCGGCA GCTGCTCTCG CTCCCACCGC CGTCCATCCG TCCACCACCT GGTGCACCAC 
CACCACCACC ATCACCACCA CTACCATCTG GGCAATGGGA CGCTGAGGGC CCCCCGGGCC 
AGCCCGGAGA TTCAGGACAA GGATGCCAGT GGGTCCCGCC GGCTCATGCT GCCACCACCC 
TCGACGCCAG CCCTCTCTGG GGGCCCTCCA GGGGGTGCAG AATCTGTGCA CAGCTTCTAC 
CATGCCGACT GCCACCTGGA GCCGGTCCGC TGCCAGGCAC CCCCTCCCAG GTCACCATCT 
GAGGCGTCGG GCAGGACTGT GGGCAGCGGG AAGGTGTACC CCACTGTGCA CACCAGCCCT 
CCACCAGAGA TGCTGAAGGA GAAAGCGCTA GTGGAGGTGG CCCCCACTTC TGGACCCCCC 
ACCCTCACCA GCCTCAACAT CCCGCCCGGG CCCTACAGCT CCATGCACAA GCTACTAGAG 
ACACAGAGCA CAGGCGCCTG CCAAAGCTCT TGCAAGATCT CCAGCCCTTG CCTGAAGGCA 
GACAGTGGAG CCGGTGGCCC AGACAGCTGC CCCTACTGTG CCCGGGCGGG GGCAGGGCAG 
GTGGAGCTCG CCGGCCACGA GATGCCTGAC TCAGACAGCG AGGCAGTTTA TGAGCTCACA 
CAAGACGTCC ACCACGGTGA TCTCCGGGAC CCCCACAGCC GGCACCGCCA GAGCCTGGGG 
CTGGACGTGG AGCCCAACTC TGTGCTGGCC TTCTGGAGGC TGATCTGTGA CACTTTCCGG 
AAGATTGTGG ACAGCAAGTA CTTTGGTCGG GGAATCATGA TTGCTATCCT GGTCAACACG 
CTCAGCATGG GCATCGAGTA CCATGAACAG CCCGAGGAGC TGACCAACGC CCTAGAAATT 
AGCAACATCG TCTTCACCAG CCTCTTTGCC CTGGAGATGC TGCTGAAGCT GCTGGTGTAC 
GGTCCCTTTG GCTACATCAA GAACCCCTAC AATATCTTCG ATGGCGTCAT TGTTGTCATC 
AGCGTGTGGG AGATCGTGGG CCAGCAGGGG GGTGGCCTGT CGGTGCTGCG GACCTTCCGC 
CTGATGCGGG TGCTGAAGCT GGTGCGCTTC CTGCCGGCAC TGCAGCGGCA GCTCGTGGTG 
CTCATGAAGA CCATGGACAA CGTGGCCACC TTCTGCATGC TACTCATGCT CTTCATCTTC 
ATCTTCAGCA TCCTGGGCAT GCATCTCTTT GGCTGCAAAT TTGCATCTGA GCGGGACGGG 
GACACGCTGC CGGACCGGAA GAATTTTGAC TCCTTGCTCT GGGCCATCGT CACCGTCTTT 
CAGATCCTGA CCCAGGAGGA CTGGAACAAG GTGCTCTACA ATGGAATGGC CTCCACATCA 
TCCTGGGCCG CCCTTTATTT CATTGCCCTC ATGACCTTTG GCAACTACGT GCTCTTTAAT 
CTGCTTGTCG CCATTCTGGT GGAGGGCTTC CAGGCGGAGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GATGGAGCCC CACCCCGAGG AGATGCCCCT TGCACTGGGA 
CTGGACCTGC TGACTGTGAG GAAGTCTGGG GTCAGCCGGA CACACTCCCT GCCCAATGAC 
AGCTACATGT TCCGGGACGG AGGCACCGCT GAGGGGTCCC TAGGATGCAG AAGCTGGGGG 
CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA TCTGTCCACT CGCAGCCAGC AGACACCAGC 
TACATCCTGC AGCTTCCCAA AGACACACCT CACCTGCTCC AGCCCCACAG CGCTCCCACC 
TGGGGCACCA TTCCCAAACT GCCCCCACCA GGCCGCTCCC CCTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATAAG GACTGATTCT CTGGACGTTC AGGGCCTGGG CAGCCGGGAA 
GACCTGCTGT CCGAGGTGAG TGGGCCCTCC CCACCTCTGG CCCGGGCCTC CTCCTTCTGG 
GGCCACTTGG GCGCCCAGAT GCAGCAGCAT CCCCACCGCC AGAGCAAGAT CTCGAAGCAC 
ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA GAAACCACCT GGAGCAAAGA CCCACCCGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGACC 
GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA CGGGACCTGA AGAAGTGTCA CAGCGTGGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC TGGCTGGATG AACAGAGGAG ACACTCCATC 
GCGGTCAGCT GCCTGGACGG CGGCTCCCAA CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT 
GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC CGGCCCAAGA AAAAACTCAG CCCACCTAGT 
ATCTCCATAG ACCCCCCAGA GAGTCAGGGC CCTCGGCCCC CACCCAGCCC CGGCGTCTGC 
CTCCGGCGGA GGGCTCCGTC CAGCGACTCC AGGGACCCCT CGGCCTCCGG CCCCCCTGAC 
AGCATGGCTG CCTCGCCCCC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTAGCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005624663.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624720.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X28, mRNA.

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CloneID	OCe11937
Clone ID Related Accession (Same CDS sequence)	XM_005624663.2 , XM_005624663.3
Accession Version	XM_005624663.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624663.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
TTCTCAGAGA CCAAGCAGCG GGAAAGCCAG CTGATGCGAG AGCAACGTGT GCGGTTCCTG 
TCCAACGCCA GCACCCTGGC CAGCTTCTCT GAGCCGGGCA GCTGCTACGA GGAGCTGCTC 
AAGTACCTGG TGTACATCCT CCGTAAAGCG GCCCGCAGGC TGGCCCAGGT CTCCCGGGCG 
GCGGGCGTGC GGGTCGGCCT GCTGAGCAGC CCAGTGCCCC GTGGAGGCCA GGAGCCCCAG 
TCTGGCGGCA GCTGCTCTCG CTCCCACCGC CGTCCATCCG TCCACCACCT GGTGCACCAC 
CACCACCACC ATCACCACCA CTACCATCTG GGCAATGGGA CGCTGAGGGC CCCCCGGGCC 
AGCCCGGAGA TTCAGGACAA GGATGCCAGT GGGTCCCGCC GGCTCATGCT GCCACCACCC 
TCGACGCCAG CCCTCTCTGG GGGCCCTCCA GGGGGTGCAG AATCTGTGCA CAGCTTCTAC 
CATGCCGACT GCCACCTGGA GCCGGTCCGC TGCCAGGCAC CCCCTCCCAG GTCACCATCT 
GAGGCGTCGG GCAGGACTGT GGGCAGCGGG AAGGTGTACC CCACTGTGCA CACCAGCCCT 
CCACCAGAGA TGCTGAAGGA GAAAGCGCTA GTGGAGGTGG CCCCCACTTC TGGACCCCCC 
ACCCTCACCA GCCTCAACAT CCCGCCCGGG CCCTACAGCT CCATGCACAA GCTACTAGAG 
ACACAGAGCA CAGGCGCCTG CCAAAGCTCT TGCAAGATCT CCAGCCCTTG CCTGAAGGCA 
GACAGTGGAG CCGGTGGCCC AGACAGCTGC CCCTACTGTG CCCGGGCGGG GGCAGGGCAG 
GTGGAGCTCG CCGGCCACGA GATGCCTGAC TCAGACAGCG AGGCAGTTTA TGAGCTCACA 
CAAGACGTCC ACCACGGTGA TCTCCGGGAC CCCCACAGCC GGCACCGCCA GAGCCTGGGG 
CTGGACGTGG AGCCCAACTC TGTGCTGGCC TTCTGGAGGC TGATCTGTGA CACTTTCCGG 
AAGATTGTGG ACAGCAAGTA CTTTGGTCGG GGAATCATGA TTGCTATCCT GGTCAACACG 
CTCAGCATGG GCATCGAGTA CCATGAACAG CCCGAGGAGC TGACCAACGC CCTAGAAATT 
AGCAACATCG TCTTCACCAG CCTCTTTGCC CTGGAGATGC TGCTGAAGCT GCTGGTGTAC 
GGTCCCTTTG GCTACATCAA GAACCCCTAC AATATCTTCG ATGGCGTCAT TGTTGTCATC 
AGCGTGTGGG AGATCGTGGG CCAGCAGGGG GGTGGCCTGT CGGTGCTGCG GACCTTCCGC 
CTGATGCGGG TGCTGAAGCT GGTGCGCTTC CTGCCGGCAC TGCAGCGGCA GCTCGTGGTG 
CTCATGAAGA CCATGGACAA CGTGGCCACC TTCTGCATGC TACTCATGCT CTTCATCTTC 
ATCTTCAGCA TCCTGGGCAT GCATCTCTTT GGCTGCAAAT TTGCATCTGA GCGGGACGGG 
GACACGCTGC CGGACCGGAA GAATTTTGAC TCCTTGCTCT GGGCCATCGT CACCGTCTTT 
CAGATCCTGA CCCAGGAGGA CTGGAACAAG GTGCTCTACA ATGGAATGGC CTCCACATCA 
TCCTGGGCCG CCCTTTATTT CATTGCCCTC ATGACCTTTG GCAACTACGT GCTCTTTAAT 
CTGCTTGTCG CCATTCTGGT GGAGGGCTTC CAGGCGGAGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GATGGAGCCC CACCCCGAGG AGATGCCCCT TGCACTGGGA 
CTGGACCTGC TGACTGTGAG GAAGTCTGGG GTCAGCCGGA CACACTCCCT GCCCAATGAC 
AGCTACATGT TCCGGGACGG AGGCACCGCT GAGGGGTCCC TAGGATGCAG AAGCTGGGGG 
CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA TCTGTCCACT CGCAGCCAGC AGACACCAGC 
TACATCCTGC AGCTTCCCAA AGACACACCT CACCTGCTCC AGCCCCACAG CGCTCCCACC 
TGGGGCACCA TTCCCAAACT GCCCCCACCA GGCCGCTCCC CCTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATAAG GACTGATTCT CTGGACGTTC AGGGCCTGGG CAGCCGGGAA 
GACCTGCTGT CCGAGGTGAG TGGGCCCTCC CCACCTCTGG CCCGGGCCTC CTCCTTCTGG 
GGCCACTTGG GCGCCCAGAT GCAGCAGCAT CCCCACCGCC AGAGCAAGAT CTCGAAGCAC 
ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA GAAACCACCT GGAGCAAAGA CCCACCCGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGACC 
GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA CGGGACCTGA AGAAGTGTCA CAGCGTGGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC TGGCTGGATG AACAGAGGAG ACACTCCATC 
GCGGTCAGCT GCCTGGACGG CGGCTCCCAA CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT 
GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC CGGCCCAAGA AAAAACTCAG CCCACCTAGT 
ATCTCCATAG ACCCCCCAGA GAGTCAGGGC CCTCGGCCCC CACCCAGCCC CGGCGTCTGC 
CTCCGGCGGA GGGCTCCGTC CAGCGACTCC AGGGACCCCT CGGCCTCCGG CCCCCCTGAC 
AGCATGGCTG CCTCGCCCCC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTAGCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624720.1
CDS	504..7247
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_005624663.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624663.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
TTCTCAGAGA CCAAGCAGCG GGAAAGCCAG CTGATGCGAG AGCAACGTGT GCGGTTCCTG 
TCCAACGCCA GCACCCTGGC CAGCTTCTCT GAGCCGGGCA GCTGCTACGA GGAGCTGCTC 
AAGTACCTGG TGTACATCCT CCGTAAAGCG GCCCGCAGGC TGGCCCAGGT CTCCCGGGCG 
GCGGGCGTGC GGGTCGGCCT GCTGAGCAGC CCAGTGCCCC GTGGAGGCCA GGAGCCCCAG 
TCTGGCGGCA GCTGCTCTCG CTCCCACCGC CGTCCATCCG TCCACCACCT GGTGCACCAC 
CACCACCACC ATCACCACCA CTACCATCTG GGCAATGGGA CGCTGAGGGC CCCCCGGGCC 
AGCCCGGAGA TTCAGGACAA GGATGCCAGT GGGTCCCGCC GGCTCATGCT GCCACCACCC 
TCGACGCCAG CCCTCTCTGG GGGCCCTCCA GGGGGTGCAG AATCTGTGCA CAGCTTCTAC 
CATGCCGACT GCCACCTGGA GCCGGTCCGC TGCCAGGCAC CCCCTCCCAG GTCACCATCT 
GAGGCGTCGG GCAGGACTGT GGGCAGCGGG AAGGTGTACC CCACTGTGCA CACCAGCCCT 
CCACCAGAGA TGCTGAAGGA GAAAGCGCTA GTGGAGGTGG CCCCCACTTC TGGACCCCCC 
ACCCTCACCA GCCTCAACAT CCCGCCCGGG CCCTACAGCT CCATGCACAA GCTACTAGAG 
ACACAGAGCA CAGGCGCCTG CCAAAGCTCT TGCAAGATCT CCAGCCCTTG CCTGAAGGCA 
GACAGTGGAG CCGGTGGCCC AGACAGCTGC CCCTACTGTG CCCGGGCGGG GGCAGGGCAG 
GTGGAGCTCG CCGGCCACGA GATGCCTGAC TCAGACAGCG AGGCAGTTTA TGAGCTCACA 
CAAGACGTCC ACCACGGTGA TCTCCGGGAC CCCCACAGCC GGCACCGCCA GAGCCTGGGG 
CTGGACGTGG AGCCCAACTC TGTGCTGGCC TTCTGGAGGC TGATCTGTGA CACTTTCCGG 
AAGATTGTGG ACAGCAAGTA CTTTGGTCGG GGAATCATGA TTGCTATCCT GGTCAACACG 
CTCAGCATGG GCATCGAGTA CCATGAACAG CCCGAGGAGC TGACCAACGC CCTAGAAATT 
AGCAACATCG TCTTCACCAG CCTCTTTGCC CTGGAGATGC TGCTGAAGCT GCTGGTGTAC 
GGTCCCTTTG GCTACATCAA GAACCCCTAC AATATCTTCG ATGGCGTCAT TGTTGTCATC 
AGCGTGTGGG AGATCGTGGG CCAGCAGGGG GGTGGCCTGT CGGTGCTGCG GACCTTCCGC 
CTGATGCGGG TGCTGAAGCT GGTGCGCTTC CTGCCGGCAC TGCAGCGGCA GCTCGTGGTG 
CTCATGAAGA CCATGGACAA CGTGGCCACC TTCTGCATGC TACTCATGCT CTTCATCTTC 
ATCTTCAGCA TCCTGGGCAT GCATCTCTTT GGCTGCAAAT TTGCATCTGA GCGGGACGGG 
GACACGCTGC CGGACCGGAA GAATTTTGAC TCCTTGCTCT GGGCCATCGT CACCGTCTTT 
CAGATCCTGA CCCAGGAGGA CTGGAACAAG GTGCTCTACA ATGGAATGGC CTCCACATCA 
TCCTGGGCCG CCCTTTATTT CATTGCCCTC ATGACCTTTG GCAACTACGT GCTCTTTAAT 
CTGCTTGTCG CCATTCTGGT GGAGGGCTTC CAGGCGGAGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAATCTA ATGCTGGACG ATGTAATTGC TTCCGGCAGC 
TCATCCAGCG CGGCGCCAGA GGCCCAGTGT AAACCCTACT ACTCAGACTA CTCCCGATTC 
CGGCTCCTCG TCCACCACTT GTGTACCAGC CACTATCTGG ACCTCTTCAT CACGGGTGTC 
ATTGGTCTGA ATGTGGTCAC CATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAC 
GAGGCCTTGA AGATCTGCAA CTATATCTTC ACCGTCATCT TTGTCTTGGA GTCAGTTTTT 
AAACTCGTGG CCTTTGGCTT CCGTCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TGCTGTCCAT CATGGGCATC ACGCTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGAGTGC TGCGCATTGC CCGAGTGCTG 
AAGCTGCTGA AGATGGCTGT GGGCATGCGG GCGCTGCTGG ACACAGTCAT GCAGGCCCTG 
CCTCAGGTGG GGAACCTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCCTGA CCCTCTTCCG AGTGTCCACA 
GGTGACAACT GGAACGGCAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACA 
TGCTACAATA CAGTCATCTC CCCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG ATGAAGCACC TGGAGGAAAG CAACAAGGAG 
GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG CTGGAGCTGG AGATGAAGAC GCTCAGCCCA 
CAGCCCCACT CGCCACTGGG CAGCCCCTTC CTCTGGCCGG GGGTCGAGGG CCCCGACAGC 
CCCGATAGCC CCAAGCTCAG GGCCCCGCAC ACCGCGGCCC ACACGGGAGC AGCCTCGCGC 
TTCTCCCTGG AACACCCCAC GATGGAGCCC CACCCCGAGG AGATGCCCCT TGCACTGGGA 
CTGGACCTGC TGACTGTGAG GAAGTCTGGG GTCAGCCGGA CACACTCCCT GCCCAATGAC 
AGCTACATGT TCCGGGACGG AGGCACCGCT GAGGGGTCCC TAGGATGCAG AAGCTGGGGG 
CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA TCTGTCCACT CGCAGCCAGC AGACACCAGC 
TACATCCTGC AGCTTCCCAA AGACACACCT CACCTGCTCC AGCCCCACAG CGCTCCCACC 
TGGGGCACCA TTCCCAAACT GCCCCCACCA GGCCGCTCCC CCTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATAAG GACTGATTCT CTGGACGTTC AGGGCCTGGG CAGCCGGGAA 
GACCTGCTGT CCGAGGTGAG TGGGCCCTCC CCACCTCTGG CCCGGGCCTC CTCCTTCTGG 
GGCCACTTGG GCGCCCAGAT GCAGCAGCAT CCCCACCGCC AGAGCAAGAT CTCGAAGCAC 
ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA GAAACCACCT GGAGCAAAGA CCCACCCGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGACC 
GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA CGGGACCTGA AGAAGTGTCA CAGCGTGGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC TGGCTGGATG AACAGAGGAG ACACTCCATC 
GCGGTCAGCT GCCTGGACGG CGGCTCCCAA CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT 
GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC CGGCCCAAGA AAAAACTCAG CCCACCTAGT 
ATCTCCATAG ACCCCCCAGA GAGTCAGGGC CCTCGGCCCC CACCCAGCCC CGGCGTCTGC 
CTCCGGCGGA GGGCTCCGTC CAGCGACTCC AGGGACCCCT CGGCCTCCGG CCCCCCTGAC 
AGCATGGCTG CCTCGCCCCC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTAGCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860769.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865862.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X33, mRNA.

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CloneID	OCe11939
Clone ID Related Accession (Same CDS sequence)	XM_860769.4 , XM_860769.5
Accession Version	XM_860769.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X33
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_860769.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAAGGC CCAGTGTAAA CCCTACTACT CAGACTACTC CCGATTCCGG 
CTCCTCGTCC ACCACTTGTG TACCAGCCAC TATCTGGACC TCTTCATCAC GGGTGTCATT 
GGTCTGAATG TGGTCACCAT GGCCATGGAG CACTACCAGC AGCCCCAGAT CCTGGACGAG 
GCCTTGAAGA TCTGCAACTA TATCTTCACC GTCATCTTTG TCTTGGAGTC AGTTTTTAAA 
CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC CAGGACAGGT GGAACCAGCT GGACCTGGCC 
ATCGTGCTGC TGTCCATCAT GGGCATCACG CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG 
CCCATCAACC CCACCATCAT CCGCATCATG AGAGTGCTGC GCATTGCCCG AGTGCTGAAG 
CTGCTGAAGA TGGCTGTGGG CATGCGGGCG CTGCTGGACA CAGTCATGCA GGCCCTGCCT 
CAGGTGGGGA ACCTGGGACT TCTCTTCATG TTGTTGTTTT TCATCTTTGC AGCTCTGGGT 
GTGGAGCTCT TTGGAGACCT GGAGTGTGAT GAGACGCACC CCTGCGAGGG CCTGGGCCGG 
CATGCCACCT TTCGGAACTT TGGCATGGCC TTCCTGACCC TCTTCCGAGT GTCCACAGGT 
GACAACTGGA ACGGCATTAT GAAGGACACC CTCCGGGACT GTGACCAGGA GTCCACATGC 
TACAATACAG TCATCTCCCC CATCTACTTC GTGTCCTTCG TGCTGACGGC CCAGTTTGTG 
CTGGTCAACG TGGTGATCGC TGTGCTGATG AAGCACCTGG AGGAAAGCAA CAAGGAGGCC 
AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG GAGCTGGAGA TGAAGACGCT CAGCCCACAG 
CCCCACTCGC CACTGGGCAG CCCCTTCCTC TGGCCGGGGG TCGAGGGCCC CGACAGCCCC 
GATAGCCCCA AGCTCAGGGC CCCGCACACC GCGGCCCACA CGGGAGCAGC CTCGCGCTTC 
TCCCTGGAAC ACCCCACGAT GGAGCCCCAC CCCGAGGAGA TGCCCCTTGC ACTGGGACTG 
GACCTGCTGA CTGTGAGGAA GTCTGGGGTC AGCCGGACAC ACTCCCTGCC CAATGACAGC 
TACATGTTCC GGGACGGAGG CACCGCTGAG GGGTCCCTAG GATGCAGAAG CTGGGGGCTC 
CCCAAAGCTC AGTCAGGCTC TGTCTTATCT GTCCACTCGC AGCCAGCAGA CACCAGCTAC 
ATCCTGCAGC TTCCCAAAGA CACACCTCAC CTGCTCCAGC CCCACAGCGC TCCCACCTGG 
GGCACCATTC CCAAACTGCC CCCACCAGGC CGCTCCCCCT TGGCTCAGAG GCCACTCAGG 
CGCCAGGCAG CAATAAGGAC TGATTCTCTG GACGTTCAGG GCCTGGGCAG CCGGGAAGAC 
CTGCTGTCCG AGGTGAGTGG GCCCTCCCCA CCTCTGGCCC GGGCCTCCTC CTTCTGGGGC 
CACTTGGGCG CCCAGATGCA GCAGCATCCC CACCGCCAGA GCAAGATCTC GAAGCACATG 
CCCCCGCCAG CCCCCCGCCC AGGCTCAGAA ACCACCTGGA GCAAAGACCC ACCCGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGACCGTG 
GGCAGCCAGG AGGAGCCCCC GTCCCCACGG GACCTGAAGA AGTGTCACAG CGTGGAGGCC 
CAGAGCTGCC GGCGCAGGCC TGCGTCCTGG CTGGATGAAC AGAGGAGACA CTCCATCGCG 
GTCAGCTGCC TGGACGGCGG CTCCCAACCC AGCCTGGGCC CCGGCCCCTC TAGCCTTGGC 
GGCCAGCCTC TTGGGGGACC TGGAAGCCGG CCCAAGAAAA AACTCAGCCC ACCTAGTATC 
TCCATAGACC CCCCAGAGAG TCAGGGCCCT CGGCCCCCAC CCAGCCCCGG CGTCTGCCTC 
CGGCGGAGGG CTCCGTCCAG CGACTCCAGG GACCCCTCGG CCTCCGGCCC CCCTGACAGC 
ATGGCTGCCT CGCCCCCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT AGCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865862.1
CDS	527..7267
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860769.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860769.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAAGGC CCAGTGTAAA CCCTACTACT CAGACTACTC CCGATTCCGG 
CTCCTCGTCC ACCACTTGTG TACCAGCCAC TATCTGGACC TCTTCATCAC GGGTGTCATT 
GGTCTGAATG TGGTCACCAT GGCCATGGAG CACTACCAGC AGCCCCAGAT CCTGGACGAG 
GCCTTGAAGA TCTGCAACTA TATCTTCACC GTCATCTTTG TCTTGGAGTC AGTTTTTAAA 
CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC CAGGACAGGT GGAACCAGCT GGACCTGGCC 
ATCGTGCTGC TGTCCATCAT GGGCATCACG CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG 
CCCATCAACC CCACCATCAT CCGCATCATG AGAGTGCTGC GCATTGCCCG AGTGCTGAAG 
CTGCTGAAGA TGGCTGTGGG CATGCGGGCG CTGCTGGACA CAGTCATGCA GGCCCTGCCT 
CAGGTGGGGA ACCTGGGACT TCTCTTCATG TTGTTGTTTT TCATCTTTGC AGCTCTGGGT 
GTGGAGCTCT TTGGAGACCT GGAGTGTGAT GAGACGCACC CCTGCGAGGG CCTGGGCCGG 
CATGCCACCT TTCGGAACTT TGGCATGGCC TTCCTGACCC TCTTCCGAGT GTCCACAGGT 
GACAACTGGA ACGGCATTAT GAAGGACACC CTCCGGGACT GTGACCAGGA GTCCACATGC 
TACAATACAG TCATCTCCCC CATCTACTTC GTGTCCTTCG TGCTGACGGC CCAGTTTGTG 
CTGGTCAACG TGGTGATCGC TGTGCTGATG AAGCACCTGG AGGAAAGCAA CAAGGAGGCC 
AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG GAGCTGGAGA TGAAGACGCT CAGCCCACAG 
CCCCACTCGC CACTGGGCAG CCCCTTCCTC TGGCCGGGGG TCGAGGGCCC CGACAGCCCC 
GATAGCCCCA AGCTCAGGGC CCCGCACACC GCGGCCCACA CGGGAGCAGC CTCGCGCTTC 
TCCCTGGAAC ACCCCACGAT GGAGCCCCAC CCCGAGGAGA TGCCCCTTGC ACTGGGACTG 
GACCTGCTGA CTGTGAGGAA GTCTGGGGTC AGCCGGACAC ACTCCCTGCC CAATGACAGC 
TACATGTTCC GGGACGGAGG CACCGCTGAG GGGTCCCTAG GATGCAGAAG CTGGGGGCTC 
CCCAAAGCTC AGTCAGGCTC TGTCTTATCT GTCCACTCGC AGCCAGCAGA CACCAGCTAC 
ATCCTGCAGC TTCCCAAAGA CACACCTCAC CTGCTCCAGC CCCACAGCGC TCCCACCTGG 
GGCACCATTC CCAAACTGCC CCCACCAGGC CGCTCCCCCT TGGCTCAGAG GCCACTCAGG 
CGCCAGGCAG CAATAAGGAC TGATTCTCTG GACGTTCAGG GCCTGGGCAG CCGGGAAGAC 
CTGCTGTCCG AGGTGAGTGG GCCCTCCCCA CCTCTGGCCC GGGCCTCCTC CTTCTGGGGC 
CACTTGGGCG CCCAGATGCA GCAGCATCCC CACCGCCAGA GCAAGATCTC GAAGCACATG 
CCCCCGCCAG CCCCCCGCCC AGGCTCAGAA ACCACCTGGA GCAAAGACCC ACCCGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGACCGTG 
GGCAGCCAGG AGGAGCCCCC GTCCCCACGG GACCTGAAGA AGTGTCACAG CGTGGAGGCC 
CAGAGCTGCC GGCGCAGGCC TGCGTCCTGG CTGGATGAAC AGAGGAGACA CTCCATCGCG 
GTCAGCTGCC TGGACGGCGG CTCCCAACCC AGCCTGGGCC CCGGCCCCTC TAGCCTTGGC 
GGCCAGCCTC TTGGGGGACC TGGAAGCCGG CCCAAGAAAA AACTCAGCCC ACCTAGTATC 
TCCATAGACC CCCCAGAGAG TCAGGGCCCT CGGCCCCCAC CCAGCCCCGG CGTCTGCCTC 
CGGCGGAGGG CTCCGTCCAG CGACTCCAGG GACCCCTCGG CCTCCGGCCC CCCTGACAGC 
ATGGCTGCCT CGCCCCCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT AGCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_860769.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_865862.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X29, mRNA.

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CloneID	OCe11939
Clone ID Related Accession (Same CDS sequence)	XM_860769.4 , XM_860769.5
Accession Version	XM_860769.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_860769.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAAGGC CCAGTGTAAA CCCTACTACT CAGACTACTC CCGATTCCGG 
CTCCTCGTCC ACCACTTGTG TACCAGCCAC TATCTGGACC TCTTCATCAC GGGTGTCATT 
GGTCTGAATG TGGTCACCAT GGCCATGGAG CACTACCAGC AGCCCCAGAT CCTGGACGAG 
GCCTTGAAGA TCTGCAACTA TATCTTCACC GTCATCTTTG TCTTGGAGTC AGTTTTTAAA 
CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC CAGGACAGGT GGAACCAGCT GGACCTGGCC 
ATCGTGCTGC TGTCCATCAT GGGCATCACG CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG 
CCCATCAACC CCACCATCAT CCGCATCATG AGAGTGCTGC GCATTGCCCG AGTGCTGAAG 
CTGCTGAAGA TGGCTGTGGG CATGCGGGCG CTGCTGGACA CAGTCATGCA GGCCCTGCCT 
CAGGTGGGGA ACCTGGGACT TCTCTTCATG TTGTTGTTTT TCATCTTTGC AGCTCTGGGT 
GTGGAGCTCT TTGGAGACCT GGAGTGTGAT GAGACGCACC CCTGCGAGGG CCTGGGCCGG 
CATGCCACCT TTCGGAACTT TGGCATGGCC TTCCTGACCC TCTTCCGAGT GTCCACAGGT 
GACAACTGGA ACGGCATTAT GAAGGACACC CTCCGGGACT GTGACCAGGA GTCCACATGC 
TACAATACAG TCATCTCCCC CATCTACTTC GTGTCCTTCG TGCTGACGGC CCAGTTTGTG 
CTGGTCAACG TGGTGATCGC TGTGCTGATG AAGCACCTGG AGGAAAGCAA CAAGGAGGCC 
AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG GAGCTGGAGA TGAAGACGCT CAGCCCACAG 
CCCCACTCGC CACTGGGCAG CCCCTTCCTC TGGCCGGGGG TCGAGGGCCC CGACAGCCCC 
GATAGCCCCA AGCTCAGGGC CCCGCACACC GCGGCCCACA CGGGAGCAGC CTCGCGCTTC 
TCCCTGGAAC ACCCCACGAT GGAGCCCCAC CCCGAGGAGA TGCCCCTTGC ACTGGGACTG 
GACCTGCTGA CTGTGAGGAA GTCTGGGGTC AGCCGGACAC ACTCCCTGCC CAATGACAGC 
TACATGTTCC GGGACGGAGG CACCGCTGAG GGGTCCCTAG GATGCAGAAG CTGGGGGCTC 
CCCAAAGCTC AGTCAGGCTC TGTCTTATCT GTCCACTCGC AGCCAGCAGA CACCAGCTAC 
ATCCTGCAGC TTCCCAAAGA CACACCTCAC CTGCTCCAGC CCCACAGCGC TCCCACCTGG 
GGCACCATTC CCAAACTGCC CCCACCAGGC CGCTCCCCCT TGGCTCAGAG GCCACTCAGG 
CGCCAGGCAG CAATAAGGAC TGATTCTCTG GACGTTCAGG GCCTGGGCAG CCGGGAAGAC 
CTGCTGTCCG AGGTGAGTGG GCCCTCCCCA CCTCTGGCCC GGGCCTCCTC CTTCTGGGGC 
CACTTGGGCG CCCAGATGCA GCAGCATCCC CACCGCCAGA GCAAGATCTC GAAGCACATG 
CCCCCGCCAG CCCCCCGCCC AGGCTCAGAA ACCACCTGGA GCAAAGACCC ACCCGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGACCGTG 
GGCAGCCAGG AGGAGCCCCC GTCCCCACGG GACCTGAAGA AGTGTCACAG CGTGGAGGCC 
CAGAGCTGCC GGCGCAGGCC TGCGTCCTGG CTGGATGAAC AGAGGAGACA CTCCATCGCG 
GTCAGCTGCC TGGACGGCGG CTCCCAACCC AGCCTGGGCC CCGGCCCCTC TAGCCTTGGC 
GGCCAGCCTC TTGGGGGACC TGGAAGCCGG CCCAAGAAAA AACTCAGCCC ACCTAGTATC 
TCCATAGACC CCCCAGAGAG TCAGGGCCCT CGGCCCCCAC CCAGCCCCGG CGTCTGCCTC 
CGGCGGAGGG CTCCGTCCAG CGACTCCAGG GACCCCTCGG CCTCCGGCCC CCCTGACAGC 
ATGGCTGCCT CGCCCCCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT AGCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_865862.1
CDS	504..7244
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_860769.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_860769.5

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAAGGC CCAGTGTAAA CCCTACTACT CAGACTACTC CCGATTCCGG 
CTCCTCGTCC ACCACTTGTG TACCAGCCAC TATCTGGACC TCTTCATCAC GGGTGTCATT 
GGTCTGAATG TGGTCACCAT GGCCATGGAG CACTACCAGC AGCCCCAGAT CCTGGACGAG 
GCCTTGAAGA TCTGCAACTA TATCTTCACC GTCATCTTTG TCTTGGAGTC AGTTTTTAAA 
CTCGTGGCCT TTGGCTTCCG TCGGTTCTTC CAGGACAGGT GGAACCAGCT GGACCTGGCC 
ATCGTGCTGC TGTCCATCAT GGGCATCACG CTGGAGGAGA TTGAGGTCAA TGCCTCGCTG 
CCCATCAACC CCACCATCAT CCGCATCATG AGAGTGCTGC GCATTGCCCG AGTGCTGAAG 
CTGCTGAAGA TGGCTGTGGG CATGCGGGCG CTGCTGGACA CAGTCATGCA GGCCCTGCCT 
CAGGTGGGGA ACCTGGGACT TCTCTTCATG TTGTTGTTTT TCATCTTTGC AGCTCTGGGT 
GTGGAGCTCT TTGGAGACCT GGAGTGTGAT GAGACGCACC CCTGCGAGGG CCTGGGCCGG 
CATGCCACCT TTCGGAACTT TGGCATGGCC TTCCTGACCC TCTTCCGAGT GTCCACAGGT 
GACAACTGGA ACGGCATTAT GAAGGACACC CTCCGGGACT GTGACCAGGA GTCCACATGC 
TACAATACAG TCATCTCCCC CATCTACTTC GTGTCCTTCG TGCTGACGGC CCAGTTTGTG 
CTGGTCAACG TGGTGATCGC TGTGCTGATG AAGCACCTGG AGGAAAGCAA CAAGGAGGCC 
AAAGAGGAGG CCGAGCTGGA GGCTGAGCTG GAGCTGGAGA TGAAGACGCT CAGCCCACAG 
CCCCACTCGC CACTGGGCAG CCCCTTCCTC TGGCCGGGGG TCGAGGGCCC CGACAGCCCC 
GATAGCCCCA AGCTCAGGGC CCCGCACACC GCGGCCCACA CGGGAGCAGC CTCGCGCTTC 
TCCCTGGAAC ACCCCACGAT GGAGCCCCAC CCCGAGGAGA TGCCCCTTGC ACTGGGACTG 
GACCTGCTGA CTGTGAGGAA GTCTGGGGTC AGCCGGACAC ACTCCCTGCC CAATGACAGC 
TACATGTTCC GGGACGGAGG CACCGCTGAG GGGTCCCTAG GATGCAGAAG CTGGGGGCTC 
CCCAAAGCTC AGTCAGGCTC TGTCTTATCT GTCCACTCGC AGCCAGCAGA CACCAGCTAC 
ATCCTGCAGC TTCCCAAAGA CACACCTCAC CTGCTCCAGC CCCACAGCGC TCCCACCTGG 
GGCACCATTC CCAAACTGCC CCCACCAGGC CGCTCCCCCT TGGCTCAGAG GCCACTCAGG 
CGCCAGGCAG CAATAAGGAC TGATTCTCTG GACGTTCAGG GCCTGGGCAG CCGGGAAGAC 
CTGCTGTCCG AGGTGAGTGG GCCCTCCCCA CCTCTGGCCC GGGCCTCCTC CTTCTGGGGC 
CACTTGGGCG CCCAGATGCA GCAGCATCCC CACCGCCAGA GCAAGATCTC GAAGCACATG 
CCCCCGCCAG CCCCCCGCCC AGGCTCAGAA ACCACCTGGA GCAAAGACCC ACCCGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGACCGTG 
GGCAGCCAGG AGGAGCCCCC GTCCCCACGG GACCTGAAGA AGTGTCACAG CGTGGAGGCC 
CAGAGCTGCC GGCGCAGGCC TGCGTCCTGG CTGGATGAAC AGAGGAGACA CTCCATCGCG 
GTCAGCTGCC TGGACGGCGG CTCCCAACCC AGCCTGGGCC CCGGCCCCTC TAGCCTTGGC 
GGCCAGCCTC TTGGGGGACC TGGAAGCCGG CCCAAGAAAA AACTCAGCCC ACCTAGTATC 
TCCATAGACC CCCCAGAGAG TCAGGGCCCT CGGCCCCCAC CCAGCCCCGG CGTCTGCCTC 
CGGCGGAGGG CTCCGTCCAG CGACTCCAGG GACCCCTCGG CCTCCGGCCC CCCTGACAGC 
ATGGCTGCCT CGCCCCCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT AGCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_022423104.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022278812.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X1, mRNA.

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CloneID	OCe290662
Clone ID Related Accession (Same CDS sequence)	XM_022423104.1
Accession Version	XM_022423104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGGCAGCTC ATACTGGTGG 
GGGCAGGGAG ATGGGCTATG GCCGGAGCTC TCAGAGCAGC AGACTTGTCC AACGCTACCC 
CAGGAGGTCT CGACTTCTTC GAGCACAGAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG GACAGACAGC TGTTTGACAC CATATCCCTG 
CTGATCCAGG GCTCCCTGGA AGGGGAGCTG AAGCTGATGG ACGAGCTGGC AGGCCCAGGG 
GGCCAGCCCT CTGCCTTCCC TCCCGCCCGC AGCCTGGGCG GCTCCGACCC ACAGATCCCT 
CTAGCTGAGA TGGAGGCTCT GTCTCTGACG TCAGAGATTG TGTCTGAACC GTCCCACTCT 
CTAGCTCTGA CGGATGACTC TTTGCCTGAT GACACTCACA CACTCTTACC TAGTGCCCTG 
GACAGCAATA TGGAGCCCCA CCCCGAGGAG ATGCCCCTTG CACTGGGACT GGACCTGCTG 
ACTGTGAGGA AGTCTGGGGT CAGCCGGACA CACTCCCTGC CCAATGACAG CTACATGTTC 
CGGGACGGAG GCACCGCTGA GGGGTCCCTA GGATGCAGAA GCTGGGGGCT CCCCAAAGCT 
CAGTCAGGCT CTGTCTTATC TGTCCACTCG CAGCCAGCAG ACACCAGCTA CATCCTGCAG 
CTTCCCAAAG ACACACCTCA CCTGCTCCAG CCCCACAGCG CTCCCACCTG GGGCACCATT 
CCCAAACTGC CCCCACCAGG CCGCTCCCCC TTGGCTCAGA GGCCACTCAG GCGCCAGGCA 
GCAATAAGGA CTGATTCTCT GGACGTTCAG GGCCTGGGCA GCCGGGAAGA CCTGCTGTCC 
GAGGTGAGTG GGCCCTCCCC ACCTCTGGCC CGGGCCTCCT CCTTCTGGGG CCACTTGGGC 
GCCCAGATGC AGCAGCATCC CCACCGCCAG AGCAAGATCT CGAAGCACAT GCCCCCGCCA 
GCCCCCCGCC CAGGCTCAGA AACCACCTGG AGCAAAGACC CACCCGAGAC CAGAAGCAGC 
TTAGAGCTGG ACACGGAGCT GAGCTGGATT TCAGGAGACC TCCTGACCGT GGGCAGCCAG 
GAGGAGCCCC CGTCCCCACG GGACCTGAAG AAGTGTCACA GCGTGGAGGC CCAGAGCTGC 
CGGCGCAGGC CTGCGTCCTG GCTGGATGAA CAGAGGAGAC ACTCCATCGC GGTCAGCTGC 
CTGGACGGCG GCTCCCAACC CAGCCTGGGC CCCGGCCCCT CTAGCCTTGG CGGCCAGCCT 
CTTGGGGGAC CTGGAAGCCG GCCCAAGAAA AAACTCAGCC CACCTAGTAT CTCCATAGAC 
CCCCCAGAGA GTCAGGGCCC TCGGCCCCCA CCCAGCCCCG GCGTCTGCCT CCGGCGGAGG 
GCTCCGTCCA GCGACTCCAG GGACCCCTCG GCCTCCGGCC CCCCTGACAG CATGGCTGCC 
TCGCCCCCCC CAAAGAAAGA CGTGCTGAGT CTCTCTGGTT TAGCCTCTGA CCCAGCAGAC 
CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022278812.1
CDS	505..7776
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPGSSYWWGQGDGLWPELSEQQTCPTLPQEVSTSSSTEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_022423104.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423104.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGGCAGCTC ATACTGGTGG 
GGGCAGGGAG ATGGGCTATG GCCGGAGCTC TCAGAGCAGC AGACTTGTCC AACGCTACCC 
CAGGAGGTCT CGACTTCTTC GAGCACAGAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG GACAGACAGC TGTTTGACAC CATATCCCTG 
CTGATCCAGG GCTCCCTGGA AGGGGAGCTG AAGCTGATGG ACGAGCTGGC AGGCCCAGGG 
GGCCAGCCCT CTGCCTTCCC TCCCGCCCGC AGCCTGGGCG GCTCCGACCC ACAGATCCCT 
CTAGCTGAGA TGGAGGCTCT GTCTCTGACG TCAGAGATTG TGTCTGAACC GTCCCACTCT 
CTAGCTCTGA CGGATGACTC TTTGCCTGAT GACACTCACA CACTCTTACC TAGTGCCCTG 
GACAGCAATA TGGAGCCCCA CCCCGAGGAG ATGCCCCTTG CACTGGGACT GGACCTGCTG 
ACTGTGAGGA AGTCTGGGGT CAGCCGGACA CACTCCCTGC CCAATGACAG CTACATGTTC 
CGGGACGGAG GCACCGCTGA GGGGTCCCTA GGATGCAGAA GCTGGGGGCT CCCCAAAGCT 
CAGTCAGGCT CTGTCTTATC TGTCCACTCG CAGCCAGCAG ACACCAGCTA CATCCTGCAG 
CTTCCCAAAG ACACACCTCA CCTGCTCCAG CCCCACAGCG CTCCCACCTG GGGCACCATT 
CCCAAACTGC CCCCACCAGG CCGCTCCCCC TTGGCTCAGA GGCCACTCAG GCGCCAGGCA 
GCAATAAGGA CTGATTCTCT GGACGTTCAG GGCCTGGGCA GCCGGGAAGA CCTGCTGTCC 
GAGGTGAGTG GGCCCTCCCC ACCTCTGGCC CGGGCCTCCT CCTTCTGGGG CCACTTGGGC 
GCCCAGATGC AGCAGCATCC CCACCGCCAG AGCAAGATCT CGAAGCACAT GCCCCCGCCA 
GCCCCCCGCC CAGGCTCAGA AACCACCTGG AGCAAAGACC CACCCGAGAC CAGAAGCAGC 
TTAGAGCTGG ACACGGAGCT GAGCTGGATT TCAGGAGACC TCCTGACCGT GGGCAGCCAG 
GAGGAGCCCC CGTCCCCACG GGACCTGAAG AAGTGTCACA GCGTGGAGGC CCAGAGCTGC 
CGGCGCAGGC CTGCGTCCTG GCTGGATGAA CAGAGGAGAC ACTCCATCGC GGTCAGCTGC 
CTGGACGGCG GCTCCCAACC CAGCCTGGGC CCCGGCCCCT CTAGCCTTGG CGGCCAGCCT 
CTTGGGGGAC CTGGAAGCCG GCCCAAGAAA AAACTCAGCC CACCTAGTAT CTCCATAGAC 
CCCCCAGAGA GTCAGGGCCC TCGGCCCCCA CCCAGCCCCG GCGTCTGCCT CCGGCGGAGG 
GCTCCGTCCA GCGACTCCAG GGACCCCTCG GCCTCCGGCC CCCCTGACAG CATGGCTGCC 
TCGCCCCCCC CAAAGAAAGA CGTGCTGAGT CTCTCTGGTT TAGCCTCTGA CCCAGCAGAC 
CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_022423105.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022278813.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X5, mRNA.

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CloneID	OCe290663
Clone ID Related Accession (Same CDS sequence)	XM_022423105.1
Accession Version	XM_022423105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGGCAGCTC ATACTGGTGG 
GGGCAGGGAG ATGGGCTATG GCCGGAGCTC TCAGAGCAGC AGACTTGTCC AACGCTACCC 
CAGGAGGTCT CGACTTCTTC GAGCACAGAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT 
CTGACGTCAG AGATTGTGTC TGAACCGTCC CACTCTCTAG CTCTGACGGA TGACTCTTTG 
CCTGATGACA CTCACACACT CTTACCTAGT GCCCTGGACA GCAATATGGA GCCCCACCCC 
GAGGAGATGC CCCTTGCACT GGGACTGGAC CTGCTGACTG TGAGGAAGTC TGGGGTCAGC 
CGGACACACT CCCTGCCCAA TGACAGCTAC ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG 
TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC AAAGCTCAGT CAGGCTCTGT CTTATCTGTC 
CACTCGCAGC CAGCAGACAC CAGCTACATC CTGCAGCTTC CCAAAGACAC ACCTCACCTG 
CTCCAGCCCC ACAGCGCTCC CACCTGGGGC ACCATTCCCA AACTGCCCCC ACCAGGCCGC 
TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TAAGGACTGA TTCTCTGGAC 
GTTCAGGGCC TGGGCAGCCG GGAAGACCTG CTGTCCGAGG TGAGTGGGCC CTCCCCACCT 
CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC TTGGGCGCCC AGATGCAGCA GCATCCCCAC 
CGCCAGAGCA AGATCTCGAA GCACATGCCC CCGCCAGCCC CCCGCCCAGG CTCAGAAACC 
ACCTGGAGCA AAGACCCACC CGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GACCGTGGGC AGCCAGGAGG AGCCCCCGTC CCCACGGGAC 
CTGAAGAAGT GTCACAGCGT GGAGGCCCAG AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG 
GATGAACAGA GGAGACACTC CATCGCGGTC AGCTGCCTGG ACGGCGGCTC CCAACCCAGC 
CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC CAGCCTCTTG GGGGACCTGG AAGCCGGCCC 
AAGAAAAAAC TCAGCCCACC TAGTATCTCC ATAGACCCCC CAGAGAGTCA GGGCCCTCGG 
CCCCCACCCA GCCCCGGCGT CTGCCTCCGG CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC 
CCCTCGGCCT CCGGCCCCCC TGACAGCATG GCTGCCTCGC CCCCCCCAAA GAAAGACGTG 
CTGAGTCTCT CTGGTTTAGC CTCTGACCCA GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022278813.1
CDS	505..7632
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPGSSYWWGQGDGLWPELSEQQTCPTLPQEVSTSSSTEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_022423105.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423105.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGGCAGCTC ATACTGGTGG 
GGGCAGGGAG ATGGGCTATG GCCGGAGCTC TCAGAGCAGC AGACTTGTCC AACGCTACCC 
CAGGAGGTCT CGACTTCTTC GAGCACAGAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT 
CTGACGTCAG AGATTGTGTC TGAACCGTCC CACTCTCTAG CTCTGACGGA TGACTCTTTG 
CCTGATGACA CTCACACACT CTTACCTAGT GCCCTGGACA GCAATATGGA GCCCCACCCC 
GAGGAGATGC CCCTTGCACT GGGACTGGAC CTGCTGACTG TGAGGAAGTC TGGGGTCAGC 
CGGACACACT CCCTGCCCAA TGACAGCTAC ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG 
TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC AAAGCTCAGT CAGGCTCTGT CTTATCTGTC 
CACTCGCAGC CAGCAGACAC CAGCTACATC CTGCAGCTTC CCAAAGACAC ACCTCACCTG 
CTCCAGCCCC ACAGCGCTCC CACCTGGGGC ACCATTCCCA AACTGCCCCC ACCAGGCCGC 
TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TAAGGACTGA TTCTCTGGAC 
GTTCAGGGCC TGGGCAGCCG GGAAGACCTG CTGTCCGAGG TGAGTGGGCC CTCCCCACCT 
CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC TTGGGCGCCC AGATGCAGCA GCATCCCCAC 
CGCCAGAGCA AGATCTCGAA GCACATGCCC CCGCCAGCCC CCCGCCCAGG CTCAGAAACC 
ACCTGGAGCA AAGACCCACC CGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GACCGTGGGC AGCCAGGAGG AGCCCCCGTC CCCACGGGAC 
CTGAAGAAGT GTCACAGCGT GGAGGCCCAG AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG 
GATGAACAGA GGAGACACTC CATCGCGGTC AGCTGCCTGG ACGGCGGCTC CCAACCCAGC 
CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC CAGCCTCTTG GGGGACCTGG AAGCCGGCCC 
AAGAAAAAAC TCAGCCCACC TAGTATCTCC ATAGACCCCC CAGAGAGTCA GGGCCCTCGG 
CCCCCACCCA GCCCCGGCGT CTGCCTCCGG CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC 
CCCTCGGCCT CCGGCCCCCC TGACAGCATG GCTGCCTCGC CCCCCCCAAA GAAAGACGTG 
CTGAGTCTCT CTGGTTTAGC CTCTGACCCA GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_022423107.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022278815.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X12, mRNA.

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CloneID	OCe290664
Clone ID Related Accession (Same CDS sequence)	XM_022423107.1
Accession Version	XM_022423107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGGCAGCTC ATACTGGTGG 
GGGCAGGGAG ATGGGCTATG GCCGGAGCTC TCAGAGCAGC AGACTTGTCC AACGCTACCC 
CAGGAGGTCT CGACTTCTTC GAGCACAGAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG ATGGAGCCCC ACCCCGAGGA GATGCCCCTT 
GCACTGGGAC TGGACCTGCT GACTGTGAGG AAGTCTGGGG TCAGCCGGAC ACACTCCCTG 
CCCAATGACA GCTACATGTT CCGGGACGGA GGCACCGCTG AGGGGTCCCT AGGATGCAGA 
AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC TCTGTCTTAT CTGTCCACTC GCAGCCAGCA 
GACACCAGCT ACATCCTGCA GCTTCCCAAA GACACACCTC ACCTGCTCCA GCCCCACAGC 
GCTCCCACCT GGGGCACCAT TCCCAAACTG CCCCCACCAG GCCGCTCCCC CTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATAAGG ACTGATTCTC TGGACGTTCA GGGCCTGGGC 
AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT GGGCCCTCCC CACCTCTGGC CCGGGCCTCC 
TCCTTCTGGG GCCACTTGGG CGCCCAGATG CAGCAGCATC CCCACCGCCA GAGCAAGATC 
TCGAAGCACA TGCCCCCGCC AGCCCCCCGC CCAGGCTCAG AAACCACCTG GAGCAAAGAC 
CCACCCGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGACCG TGGGCAGCCA GGAGGAGCCC CCGTCCCCAC GGGACCTGAA GAAGTGTCAC 
AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG CCTGCGTCCT GGCTGGATGA ACAGAGGAGA 
CACTCCATCG CGGTCAGCTG CCTGGACGGC GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC 
TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA CCTGGAAGCC GGCCCAAGAA AAAACTCAGC 
CCACCTAGTA TCTCCATAGA CCCCCCAGAG AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC 
GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC AGCGACTCCA GGGACCCCTC GGCCTCCGGC 
CCCCCTGACA GCATGGCTGC CTCGCCCCCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT 
TTAGCCTCTG ACCCAGCAGA CCTGGACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022278815.1
CDS	504..7496
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPGSSYWWGQGDGLWPELSEQQTCPTLPQEVSTSSSTEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_022423107.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423107.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGGCAGCTC ATACTGGTGG 
GGGCAGGGAG ATGGGCTATG GCCGGAGCTC TCAGAGCAGC AGACTTGTCC AACGCTACCC 
CAGGAGGTCT CGACTTCTTC GAGCACAGAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG ATGGAGCCCC ACCCCGAGGA GATGCCCCTT 
GCACTGGGAC TGGACCTGCT GACTGTGAGG AAGTCTGGGG TCAGCCGGAC ACACTCCCTG 
CCCAATGACA GCTACATGTT CCGGGACGGA GGCACCGCTG AGGGGTCCCT AGGATGCAGA 
AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC TCTGTCTTAT CTGTCCACTC GCAGCCAGCA 
GACACCAGCT ACATCCTGCA GCTTCCCAAA GACACACCTC ACCTGCTCCA GCCCCACAGC 
GCTCCCACCT GGGGCACCAT TCCCAAACTG CCCCCACCAG GCCGCTCCCC CTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATAAGG ACTGATTCTC TGGACGTTCA GGGCCTGGGC 
AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT GGGCCCTCCC CACCTCTGGC CCGGGCCTCC 
TCCTTCTGGG GCCACTTGGG CGCCCAGATG CAGCAGCATC CCCACCGCCA GAGCAAGATC 
TCGAAGCACA TGCCCCCGCC AGCCCCCCGC CCAGGCTCAG AAACCACCTG GAGCAAAGAC 
CCACCCGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGACCG TGGGCAGCCA GGAGGAGCCC CCGTCCCCAC GGGACCTGAA GAAGTGTCAC 
AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG CCTGCGTCCT GGCTGGATGA ACAGAGGAGA 
CACTCCATCG CGGTCAGCTG CCTGGACGGC GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC 
TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA CCTGGAAGCC GGCCCAAGAA AAAACTCAGC 
CCACCTAGTA TCTCCATAGA CCCCCCAGAG AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC 
GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC AGCGACTCCA GGGACCCCTC GGCCTCCGGC 
CCCCCTGACA GCATGGCTGC CTCGCCCCCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT 
TTAGCCTCTG ACCCAGCAGA CCTGGACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116641.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972116.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X8, mRNA.

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CloneID	OCe38390
Clone ID Related Accession (Same CDS sequence)	XM_014116641.1
Accession Version	XM_014116641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTCAGTG 
GTGTCCCTGG GAGAGCACCC AGAGCTGCGG AGGAACCTGC TGCCACCTCT TATCATCCAC 
ACGGCGGCCA CACCCATGTC GCTCCCCAAG AGTTCCAGCA CTGGCCTGGC CGAGGTGCTG 
GGCCCTGCCT CCCGCCGCAC CAGCAGCAGC GGGTCTGCTG AGCCAGGGGC GGCCCATGAG 
ATGAAGTCAC CGCCGAGCGC CCGCAGCACT CCGCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGAG CCCCCAGCCT GAAGCGGAGG 
AGCCCGAGTG GGGAGCGGCG GTCCCTGTTG TCCGGCGAGG GTCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CTGAAGAGGA GCGGGCCAGC CCAGCGGGCA GCGACCGGCG CCATGGAGGC 
TCCCTGGAGC GGGAGGCCAA GAGCTCCTTC GACCTGCCGG ACACGCTGCA AGTGCCCGGA 
CTGCACCGCA CGGCCAGTGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAG 
TCGGCCTCGG GGCGCCTGGC CCGGGCCCTG CGGCCCGACG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ACGAGGGCAA CCTGAGCAGA GGGGAGCGGA TAAGAGTGTG GATCCGAGCC 
CGGCTGCCTG CCTGCTGCCG CGAGCGTGAT TCCTGGTCCG CGTACATCTT CCCTCCTCAG 
TCCAGGTTCC GCCTCTTATG TCACCGAATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTTGTCATCA TCTTCCTCAA CTGCATCACC ATCGCCATGG AGCGTCCCAA GATCGACCCC 
CACAGTGCTG AGCGCATCTT CCTGACCCTC TCCAATTACA TCTTTACCGC AGTCTTTCTG 
GCAGAGATGA CCGTGAAGGT GGTAGCGCTG GGCTGGTGCT TCGGGGAGCA GGCGTACCTG 
CGTAGCAGCT GGAACGTTCT CGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGTTGAGGGT GCTGCGGCTG 
CTACGGACCC TGCGCCCACT CAGGGTTATT AGCAGAGCCC AGGGGCTGAA GCTGGTAGTG 
GAAACGCTGA TGTCCTCACT GAAGCCCATC GGCAACATCG TGGTCATTTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT GGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACCAACAAG TCTGACTGCA CCGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAATTT TGACAACCTC GGCCAGGCCC TGATGTCCCT GTTCGTACTG 
GCCTCCAAGG ACGGCTGGGT GGACATCATG TACGACGGGC TGGACGCCGT GGGTGTGGAC 
CAACAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATTGTGGCCT TCTTTGTCCT GAACATGTTT GTGGGTGTGG TGGTGGAGAA CTTTCACAAG 
TGTCGGCAGC ACCAGGAGGA GGAGGAGGCC CGGCGGCGAG AGGAGAAACG TCTGCGGAGA 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG ATCTAATGCT GGACGATGTA 
ATTGCTTCCG GCAGCTCATC CAGCGCGGCG CCAGAGGCCC AGTGTAAACC CTACTACTCA 
GACTACTCCC GATTCCGGCT CCTCGTCCAC CACTTGTGTA CCAGCCACTA TCTGGACCTC 
TTCATCACGG GTGTCATTGG TCTGAATGTG GTCACCATGG CCATGGAGCA CTACCAGCAG 
CCCCAGATCC TGGACGAGGC CTTGAAGATC TGCAACTATA TCTTCACCGT CATCTTTGTC 
TTGGAGTCAG TTTTTAAACT CGTGGCCTTT GGCTTCCGTC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCCAT CGTGCTGCTG TCCATCATGG GCATCACGCT GGAGGAGATT 
GAGGTCAATG CCTCGCTGCC CATCAACCCC ACCATCATCC GCATCATGAG AGTGCTGCGC 
ATTGCCCGAG TGCTGAAGCT GCTGAAGATG GCTGTGGGCA TGCGGGCGCT GCTGGACACA 
GTCATGCAGG CCCTGCCTCA GGTGGGGAAC CTGGGACTTC TCTTCATGTT GTTGTTTTTC 
ATCTTTGCAG CTCTGGGTGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACGCACCCC 
TGCGAGGGCC TGGGCCGGCA TGCCACCTTT CGGAACTTTG GCATGGCCTT CCTGACCCTC 
TTCCGAGTGT CCACAGGTGA CAACTGGAAC GGCATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACATGCTA CAATACAGTC ATCTCCCCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTGATCGCTG TGCTGATGAA GCACCTGGAG 
GAAAGCAACA AGGAGGCCAA AGAGGAGGCC GAGCTGGAGG CTGAGCTGGA GCTGGAGATG 
AAGACGCTCA GCCCACAGCC CCACTCGCCA CTGGGCAGCC CCTTCCTCTG GCCGGGGGTC 
GAGGGCCCCG ACAGCCCCGA TAGCCCCAAG CTCAGGGCCC CGCACACCGC GGCCCACACG 
GGAGCAGCCT CGCGCTTCTC CCTGGAACAC CCCACGGACA GACAGCTGTT TGACACCATA 
TCCCTGCTGA TCCAGGGCTC CCTGGAAGGG GAGCTGAAGC TGATGGACGA GCTGGCAGGC 
CCAGGGGGCC AGCCCTCTGC CTTCCCTCCC GCCCGCAGCC TGGGCGGCTC CGACCCACAG 
ATCCCTCTAG CTGAGATGGA GGCTCTGTCT CTGACGTCAG AGATTGTGTC TGAACCGTCC 
CACTCTCTAG CTCTGACGGA TGACTCTTTG CCTGATGACA CTCACACACT CTTACCTAGT 
GCCCTGGACA GCAATATGGA GCCCCACCCC GAGGAGATGC CCCTTGCACT GGGACTGGAC 
CTGCTGACTG TGAGGAAGTC TGGGGTCAGC CGGACACACT CCCTGCCCAA TGACAGCTAC 
ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC 
AAAGCTCAGT CAGGCTCTGT CTTATCTGTC CACTCGCAGC CAGCAGACAC CAGCTACATC 
CTGCAGCTTC CCAAAGACAC ACCTCACCTG CTCCAGCCCC ACAGCGCTCC CACCTGGGGC 
ACCATTCCCA AACTGCCCCC ACCAGGCCGC TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC 
CAGGCAGCAA TAAGGACTGA TTCTCTGGAC GTTCAGGGCC TGGGCAGCCG GGAAGACCTG 
CTGTCCGAGG TGAGTGGGCC CTCCCCACCT CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC 
TTGGGCGCCC AGATGCAGCA GCATCCCCAC CGCCAGAGCA AGATCTCGAA GCACATGCCC 
CCGCCAGCCC CCCGCCCAGG CTCAGAAACC ACCTGGAGCA AAGACCCACC CGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GACCGTGGGC 
AGCCAGGAGG AGCCCCCGTC CCCACGGGAC CTGAAGAAGT GTCACAGCGT GGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG GATGAACAGA GGAGACACTC CATCGCGGTC 
AGCTGCCTGG ACGGCGGCTC CCAACCCAGC CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC 
CAGCCTCTTG GGGGACCTGG AAGCCGGCCC AAGAAAAAAC TCAGCCCACC TAGTATCTCC 
ATAGACCCCC CAGAGAGTCA GGGCCCTCGG CCCCCACCCA GCCCCGGCGT CTGCCTCCGG 
CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC CCCTCGGCCT CCGGCCCCCC TGACAGCATG 
GCTGCCTCGC CCCCCCCAAA GAAAGACGTG CTGAGTCTCT CTGGTTTAGC CTCTGACCCA 
GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972116.1
CDS	527..7624
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116641.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116641.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTCAGTG 
GTGTCCCTGG GAGAGCACCC AGAGCTGCGG AGGAACCTGC TGCCACCTCT TATCATCCAC 
ACGGCGGCCA CACCCATGTC GCTCCCCAAG AGTTCCAGCA CTGGCCTGGC CGAGGTGCTG 
GGCCCTGCCT CCCGCCGCAC CAGCAGCAGC GGGTCTGCTG AGCCAGGGGC GGCCCATGAG 
ATGAAGTCAC CGCCGAGCGC CCGCAGCACT CCGCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGAG CCCCCAGCCT GAAGCGGAGG 
AGCCCGAGTG GGGAGCGGCG GTCCCTGTTG TCCGGCGAGG GTCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CTGAAGAGGA GCGGGCCAGC CCAGCGGGCA GCGACCGGCG CCATGGAGGC 
TCCCTGGAGC GGGAGGCCAA GAGCTCCTTC GACCTGCCGG ACACGCTGCA AGTGCCCGGA 
CTGCACCGCA CGGCCAGTGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAG 
TCGGCCTCGG GGCGCCTGGC CCGGGCCCTG CGGCCCGACG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ACGAGGGCAA CCTGAGCAGA GGGGAGCGGA TAAGAGTGTG GATCCGAGCC 
CGGCTGCCTG CCTGCTGCCG CGAGCGTGAT TCCTGGTCCG CGTACATCTT CCCTCCTCAG 
TCCAGGTTCC GCCTCTTATG TCACCGAATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTTGTCATCA TCTTCCTCAA CTGCATCACC ATCGCCATGG AGCGTCCCAA GATCGACCCC 
CACAGTGCTG AGCGCATCTT CCTGACCCTC TCCAATTACA TCTTTACCGC AGTCTTTCTG 
GCAGAGATGA CCGTGAAGGT GGTAGCGCTG GGCTGGTGCT TCGGGGAGCA GGCGTACCTG 
CGTAGCAGCT GGAACGTTCT CGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGTTGAGGGT GCTGCGGCTG 
CTACGGACCC TGCGCCCACT CAGGGTTATT AGCAGAGCCC AGGGGCTGAA GCTGGTAGTG 
GAAACGCTGA TGTCCTCACT GAAGCCCATC GGCAACATCG TGGTCATTTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT GGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACCAACAAG TCTGACTGCA CCGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAATTT TGACAACCTC GGCCAGGCCC TGATGTCCCT GTTCGTACTG 
GCCTCCAAGG ACGGCTGGGT GGACATCATG TACGACGGGC TGGACGCCGT GGGTGTGGAC 
CAACAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATTGTGGCCT TCTTTGTCCT GAACATGTTT GTGGGTGTGG TGGTGGAGAA CTTTCACAAG 
TGTCGGCAGC ACCAGGAGGA GGAGGAGGCC CGGCGGCGAG AGGAGAAACG TCTGCGGAGA 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG ATCTAATGCT GGACGATGTA 
ATTGCTTCCG GCAGCTCATC CAGCGCGGCG CCAGAGGCCC AGTGTAAACC CTACTACTCA 
GACTACTCCC GATTCCGGCT CCTCGTCCAC CACTTGTGTA CCAGCCACTA TCTGGACCTC 
TTCATCACGG GTGTCATTGG TCTGAATGTG GTCACCATGG CCATGGAGCA CTACCAGCAG 
CCCCAGATCC TGGACGAGGC CTTGAAGATC TGCAACTATA TCTTCACCGT CATCTTTGTC 
TTGGAGTCAG TTTTTAAACT CGTGGCCTTT GGCTTCCGTC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCCAT CGTGCTGCTG TCCATCATGG GCATCACGCT GGAGGAGATT 
GAGGTCAATG CCTCGCTGCC CATCAACCCC ACCATCATCC GCATCATGAG AGTGCTGCGC 
ATTGCCCGAG TGCTGAAGCT GCTGAAGATG GCTGTGGGCA TGCGGGCGCT GCTGGACACA 
GTCATGCAGG CCCTGCCTCA GGTGGGGAAC CTGGGACTTC TCTTCATGTT GTTGTTTTTC 
ATCTTTGCAG CTCTGGGTGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACGCACCCC 
TGCGAGGGCC TGGGCCGGCA TGCCACCTTT CGGAACTTTG GCATGGCCTT CCTGACCCTC 
TTCCGAGTGT CCACAGGTGA CAACTGGAAC GGCATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACATGCTA CAATACAGTC ATCTCCCCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTGATCGCTG TGCTGATGAA GCACCTGGAG 
GAAAGCAACA AGGAGGCCAA AGAGGAGGCC GAGCTGGAGG CTGAGCTGGA GCTGGAGATG 
AAGACGCTCA GCCCACAGCC CCACTCGCCA CTGGGCAGCC CCTTCCTCTG GCCGGGGGTC 
GAGGGCCCCG ACAGCCCCGA TAGCCCCAAG CTCAGGGCCC CGCACACCGC GGCCCACACG 
GGAGCAGCCT CGCGCTTCTC CCTGGAACAC CCCACGGACA GACAGCTGTT TGACACCATA 
TCCCTGCTGA TCCAGGGCTC CCTGGAAGGG GAGCTGAAGC TGATGGACGA GCTGGCAGGC 
CCAGGGGGCC AGCCCTCTGC CTTCCCTCCC GCCCGCAGCC TGGGCGGCTC CGACCCACAG 
ATCCCTCTAG CTGAGATGGA GGCTCTGTCT CTGACGTCAG AGATTGTGTC TGAACCGTCC 
CACTCTCTAG CTCTGACGGA TGACTCTTTG CCTGATGACA CTCACACACT CTTACCTAGT 
GCCCTGGACA GCAATATGGA GCCCCACCCC GAGGAGATGC CCCTTGCACT GGGACTGGAC 
CTGCTGACTG TGAGGAAGTC TGGGGTCAGC CGGACACACT CCCTGCCCAA TGACAGCTAC 
ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC 
AAAGCTCAGT CAGGCTCTGT CTTATCTGTC CACTCGCAGC CAGCAGACAC CAGCTACATC 
CTGCAGCTTC CCAAAGACAC ACCTCACCTG CTCCAGCCCC ACAGCGCTCC CACCTGGGGC 
ACCATTCCCA AACTGCCCCC ACCAGGCCGC TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC 
CAGGCAGCAA TAAGGACTGA TTCTCTGGAC GTTCAGGGCC TGGGCAGCCG GGAAGACCTG 
CTGTCCGAGG TGAGTGGGCC CTCCCCACCT CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC 
TTGGGCGCCC AGATGCAGCA GCATCCCCAC CGCCAGAGCA AGATCTCGAA GCACATGCCC 
CCGCCAGCCC CCCGCCCAGG CTCAGAAACC ACCTGGAGCA AAGACCCACC CGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GACCGTGGGC 
AGCCAGGAGG AGCCCCCGTC CCCACGGGAC CTGAAGAAGT GTCACAGCGT GGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG GATGAACAGA GGAGACACTC CATCGCGGTC 
AGCTGCCTGG ACGGCGGCTC CCAACCCAGC CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC 
CAGCCTCTTG GGGGACCTGG AAGCCGGCCC AAGAAAAAAC TCAGCCCACC TAGTATCTCC 
ATAGACCCCC CAGAGAGTCA GGGCCCTCGG CCCCCACCCA GCCCCGGCGT CTGCCTCCGG 
CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC CCCTCGGCCT CCGGCCCCCC TGACAGCATG 
GCTGCCTCGC CCCCCCCAAA GAAAGACGTG CTGAGTCTCT CTGGTTTAGC CTCTGACCCA 
GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116642.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972117.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X9, mRNA.

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CloneID	OCe38391
Clone ID Related Accession (Same CDS sequence)	XM_014116642.1
Accession Version	XM_014116642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGATC TAATGCTGGA CGATGTAATT 
GCTTCCGGCA GCTCATCCAG CGCGGCGCCA GAGGCCCAGT GTAAACCCTA CTACTCAGAC 
TACTCCCGAT TCCGGCTCCT CGTCCACCAC TTGTGTACCA GCCACTATCT GGACCTCTTC 
ATCACGGGTG TCATTGGTCT GAATGTGGTC ACCATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ACGAGGCCTT GAAGATCTGC AACTATATCT TCACCGTCAT CTTTGTCTTG 
GAGTCAGTTT TTAAACTCGT GGCCTTTGGC TTCCGTCGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATCGT GCTGCTGTCC ATCATGGGCA TCACGCTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGAGT GCTGCGCATT 
GCCCGAGTGC TGAAGCTGCT GAAGATGGCT GTGGGCATGC GGGCGCTGCT GGACACAGTC 
ATGCAGGCCC TGCCTCAGGT GGGGAACCTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGTGTGGA GCTCTTTGGA GACCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCCT GACCCTCTTC 
CGAGTGTCCA CAGGTGACAA CTGGAACGGC ATTATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CATGCTACAA TACAGTCATC TCCCCCATCT ACTTCGTGTC CTTCGTGCTG 
ACGGCCCAGT TTGTGCTGGT CAACGTGGTG ATCGCTGTGC TGATGAAGCA CCTGGAGGAA 
AGCAACAAGG AGGCCAAAGA GGAGGCCGAG CTGGAGGCTG AGCTGGAGCT GGAGATGAAG 
ACGCTCAGCC CACAGCCCCA CTCGCCACTG GGCAGCCCCT TCCTCTGGCC GGGGGTCGAG 
GGCCCCGACA GCCCCGATAG CCCCAAGCTC AGGGCCCCGC ACACCGCGGC CCACACGGGA 
GCAGCCTCGC GCTTCTCCCT GGAACACCCC ACGGACAGAC AGCTGTTTGA CACCATATCC 
CTGCTGATCC AGGGCTCCCT GGAAGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTCCCGCC CGCAGCCTGG GCGGCTCCGA CCCACAGATC 
CCTCTAGCTG AGATGGAGGC TCTGTCTCTG ACGTCAGAGA TTGTGTCTGA ACCGTCCCAC 
TCTCTAGCTC TGACGGATGA CTCTTTGCCT GATGACACTC ACACACTCTT ACCTAGTGCC 
CTGGACAGCA ATATGGAGCC CCACCCCGAG GAGATGCCCC TTGCACTGGG ACTGGACCTG 
CTGACTGTGA GGAAGTCTGG GGTCAGCCGG ACACACTCCC TGCCCAATGA CAGCTACATG 
TTCCGGGACG GAGGCACCGC TGAGGGGTCC CTAGGATGCA GAAGCTGGGG GCTCCCCAAA 
GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC TCGCAGCCAG CAGACACCAG CTACATCCTG 
CAGCTTCCCA AAGACACACC TCACCTGCTC CAGCCCCACA GCGCTCCCAC CTGGGGCACC 
ATTCCCAAAC TGCCCCCACC AGGCCGCTCC CCCTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAA GGACTGATTC TCTGGACGTT CAGGGCCTGG GCAGCCGGGA AGACCTGCTG 
TCCGAGGTGA GTGGGCCCTC CCCACCTCTG GCCCGGGCCT CCTCCTTCTG GGGCCACTTG 
GGCGCCCAGA TGCAGCAGCA TCCCCACCGC CAGAGCAAGA TCTCGAAGCA CATGCCCCCG 
CCAGCCCCCC GCCCAGGCTC AGAAACCACC TGGAGCAAAG ACCCACCCGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC 
CAGGAGGAGC CCCCGTCCCC ACGGGACCTG AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT GAACAGAGGA GACACTCCAT CGCGGTCAGC 
TGCCTGGACG GCGGCTCCCA ACCCAGCCTG GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG 
CCTCTTGGGG GACCTGGAAG CCGGCCCAAG AAAAAACTCA GCCCACCTAG TATCTCCATA 
GACCCCCCAG AGAGTCAGGG CCCTCGGCCC CCACCCAGCC CCGGCGTCTG CCTCCGGCGG 
AGGGCTCCGT CCAGCGACTC CAGGGACCCC TCGGCCTCCG GCCCCCCTGA CAGCATGGCT 
GCCTCGCCCC CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTAGCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972117.1
CDS	527..7621
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116642.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116642.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GGAGATGCCA CCAAGTCCGA ATCAGAGCCA 
GATTTCTTCT CACCCAGCCT GGAGGGCAAT GGGGACAGGA AGAAGCGCTT GGCCTTGGTG 
TCCCTGGGAG AGCACCCAGA GCTGCGGAGG AACCTGCTGC CACCTCTTAT CATCCACACG 
GCGGCCACAC CCATGTCGCT CCCCAAGAGT TCCAGCACTG GCCTGGCCGA GGTGCTGGGC 
CCTGCCTCCC GCCGCACCAG CAGCAGCGGG TCTGCTGAGC CAGGGGCGGC CCATGAGATG 
AAGTCACCGC CGAGCGCCCG CAGCACTCCG CACAGCCCCT GGAGTGCAGC AAGCAGCTGG 
ACCAGCAGGC GCTCCAGCCG GAACAGCCTG GGCCGAGCCC CCAGCCTGAA GCGGAGGAGC 
CCGAGTGGGG AGCGGCGGTC CCTGTTGTCC GGCGAGGGTC GGGAGAGCCA GGATGAGGAG 
GAGAGCTCTG AAGAGGAGCG GGCCAGCCCA GCGGGCAGCG ACCGGCGCCA TGGAGGCTCC 
CTGGAGCGGG AGGCCAAGAG CTCCTTCGAC CTGCCGGACA CGCTGCAAGT GCCCGGACTG 
CACCGCACGG CCAGTGGCCG CAGCTCAGCC TCTGAGCACC AGGACTGCAA CGGCAAGTCG 
GCCTCGGGGC GCCTGGCCCG GGCCCTGCGG CCCGACGACC CCCCACTGGA TGGGGATGAT 
GGCGATGACG AGGGCAACCT GAGCAGAGGG GAGCGGATAA GAGTGTGGAT CCGAGCCCGG 
CTGCCTGCCT GCTGCCGCGA GCGTGATTCC TGGTCCGCGT ACATCTTCCC TCCTCAGTCC 
AGGTTCCGCC TCTTATGTCA CCGAATCATC ACCCACAAGA TGTTTGACCA TGTGGTCCTT 
GTCATCATCT TCCTCAACTG CATCACCATC GCCATGGAGC GTCCCAAGAT CGACCCCCAC 
AGTGCTGAGC GCATCTTCCT GACCCTCTCC AATTACATCT TTACCGCAGT CTTTCTGGCA 
GAGATGACCG TGAAGGTGGT AGCGCTGGGC TGGTGCTTCG GGGAGCAGGC GTACCTGCGT 
AGCAGCTGGA ACGTTCTCGA CGGGCTGCTG GTGCTCATCT CCATCATCGA CATCCTGGTG 
TCCATGGTCT CCGACAGCGG CACCAAGATC CTGGGCATGT TGAGGGTGCT GCGGCTGCTA 
CGGACCCTGC GCCCACTCAG GGTTATTAGC AGAGCCCAGG GGCTGAAGCT GGTAGTGGAA 
ACGCTGATGT CCTCACTGAA GCCCATCGGC AACATCGTGG TCATTTGTTG TGCCTTCTTC 
ATCATATTTG GCATCCTGGG GGTGCAGCTC TTCAAAGGGA AGTTCTTCGT GTGCCAGGGC 
GAGGACACCA GGAACATCAC CAACAAGTCT GACTGCACCG AGGCCAGTTA CCGGTGGGTC 
CGGCACAAGT ACAATTTTGA CAACCTCGGC CAGGCCCTGA TGTCCCTGTT CGTACTGGCC 
TCCAAGGACG GCTGGGTGGA CATCATGTAC GACGGGCTGG ACGCCGTGGG TGTGGACCAA 
CAGCCCATCA TGAACCACAA CCCCTGGATG CTGCTGTACT TCATCTCGTT CCTGCTCATT 
GTGGCCTTCT TTGTCCTGAA CATGTTTGTG GGTGTGGTGG TGGAGAACTT TCACAAGTGT 
CGGCAGCACC AGGAGGAGGA GGAGGCCCGG CGGCGAGAGG AGAAACGTCT GCGGAGACTG 
GAGAAAAAGA GAAGGAGTAA GGAGAAGCAG ATGGCTGATC TAATGCTGGA CGATGTAATT 
GCTTCCGGCA GCTCATCCAG CGCGGCGCCA GAGGCCCAGT GTAAACCCTA CTACTCAGAC 
TACTCCCGAT TCCGGCTCCT CGTCCACCAC TTGTGTACCA GCCACTATCT GGACCTCTTC 
ATCACGGGTG TCATTGGTCT GAATGTGGTC ACCATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ACGAGGCCTT GAAGATCTGC AACTATATCT TCACCGTCAT CTTTGTCTTG 
GAGTCAGTTT TTAAACTCGT GGCCTTTGGC TTCCGTCGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATCGT GCTGCTGTCC ATCATGGGCA TCACGCTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGAGT GCTGCGCATT 
GCCCGAGTGC TGAAGCTGCT GAAGATGGCT GTGGGCATGC GGGCGCTGCT GGACACAGTC 
ATGCAGGCCC TGCCTCAGGT GGGGAACCTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGTGTGGA GCTCTTTGGA GACCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCCT GACCCTCTTC 
CGAGTGTCCA CAGGTGACAA CTGGAACGGC ATTATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CATGCTACAA TACAGTCATC TCCCCCATCT ACTTCGTGTC CTTCGTGCTG 
ACGGCCCAGT TTGTGCTGGT CAACGTGGTG ATCGCTGTGC TGATGAAGCA CCTGGAGGAA 
AGCAACAAGG AGGCCAAAGA GGAGGCCGAG CTGGAGGCTG AGCTGGAGCT GGAGATGAAG 
ACGCTCAGCC CACAGCCCCA CTCGCCACTG GGCAGCCCCT TCCTCTGGCC GGGGGTCGAG 
GGCCCCGACA GCCCCGATAG CCCCAAGCTC AGGGCCCCGC ACACCGCGGC CCACACGGGA 
GCAGCCTCGC GCTTCTCCCT GGAACACCCC ACGGACAGAC AGCTGTTTGA CACCATATCC 
CTGCTGATCC AGGGCTCCCT GGAAGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTCCCGCC CGCAGCCTGG GCGGCTCCGA CCCACAGATC 
CCTCTAGCTG AGATGGAGGC TCTGTCTCTG ACGTCAGAGA TTGTGTCTGA ACCGTCCCAC 
TCTCTAGCTC TGACGGATGA CTCTTTGCCT GATGACACTC ACACACTCTT ACCTAGTGCC 
CTGGACAGCA ATATGGAGCC CCACCCCGAG GAGATGCCCC TTGCACTGGG ACTGGACCTG 
CTGACTGTGA GGAAGTCTGG GGTCAGCCGG ACACACTCCC TGCCCAATGA CAGCTACATG 
TTCCGGGACG GAGGCACCGC TGAGGGGTCC CTAGGATGCA GAAGCTGGGG GCTCCCCAAA 
GCTCAGTCAG GCTCTGTCTT ATCTGTCCAC TCGCAGCCAG CAGACACCAG CTACATCCTG 
CAGCTTCCCA AAGACACACC TCACCTGCTC CAGCCCCACA GCGCTCCCAC CTGGGGCACC 
ATTCCCAAAC TGCCCCCACC AGGCCGCTCC CCCTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAA GGACTGATTC TCTGGACGTT CAGGGCCTGG GCAGCCGGGA AGACCTGCTG 
TCCGAGGTGA GTGGGCCCTC CCCACCTCTG GCCCGGGCCT CCTCCTTCTG GGGCCACTTG 
GGCGCCCAGA TGCAGCAGCA TCCCCACCGC CAGAGCAAGA TCTCGAAGCA CATGCCCCCG 
CCAGCCCCCC GCCCAGGCTC AGAAACCACC TGGAGCAAAG ACCCACCCGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGAC CGTGGGCAGC 
CAGGAGGAGC CCCCGTCCCC ACGGGACCTG AAGAAGTGTC ACAGCGTGGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGCGTC CTGGCTGGAT GAACAGAGGA GACACTCCAT CGCGGTCAGC 
TGCCTGGACG GCGGCTCCCA ACCCAGCCTG GGCCCCGGCC CCTCTAGCCT TGGCGGCCAG 
CCTCTTGGGG GACCTGGAAG CCGGCCCAAG AAAAAACTCA GCCCACCTAG TATCTCCATA 
GACCCCCCAG AGAGTCAGGG CCCTCGGCCC CCACCCAGCC CCGGCGTCTG CCTCCGGCGG 
AGGGCTCCGT CCAGCGACTC CAGGGACCCC TCGGCCTCCG GCCCCCCTGA CAGCATGGCT 
GCCTCGCCCC CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTAGCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116643.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972118.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X11, mRNA.

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CloneID	OCe38392
Clone ID Related Accession (Same CDS sequence)	XM_014116643.1 , XM_014116643.2
Accession Version	XM_014116643.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAAGGCC CAGTGTAAAC CCTACTACTC AGACTACTCC 
CGATTCCGGC TCCTCGTCCA CCACTTGTGT ACCAGCCACT ATCTGGACCT CTTCATCACG 
GGTGTCATTG GTCTGAATGT GGTCACCATG GCCATGGAGC ACTACCAGCA GCCCCAGATC 
CTGGACGAGG CCTTGAAGAT CTGCAACTAT ATCTTCACCG TCATCTTTGT CTTGGAGTCA 
GTTTTTAAAC TCGTGGCCTT TGGCTTCCGT CGGTTCTTCC AGGACAGGTG GAACCAGCTG 
GACCTGGCCA TCGTGCTGCT GTCCATCATG GGCATCACGC TGGAGGAGAT TGAGGTCAAT 
GCCTCGCTGC CCATCAACCC CACCATCATC CGCATCATGA GAGTGCTGCG CATTGCCCGA 
GTGCTGAAGC TGCTGAAGAT GGCTGTGGGC ATGCGGGCGC TGCTGGACAC AGTCATGCAG 
GCCCTGCCTC AGGTGGGGAA CCTGGGACTT CTCTTCATGT TGTTGTTTTT CATCTTTGCA 
GCTCTGGGTG TGGAGCTCTT TGGAGACCTG GAGTGTGATG AGACGCACCC CTGCGAGGGC 
CTGGGCCGGC ATGCCACCTT TCGGAACTTT GGCATGGCCT TCCTGACCCT CTTCCGAGTG 
TCCACAGGTG ACAACTGGAA CGGCATTATG AAGGACACCC TCCGGGACTG TGACCAGGAG 
TCCACATGCT ACAATACAGT CATCTCCCCC ATCTACTTCG TGTCCTTCGT GCTGACGGCC 
CAGTTTGTGC TGGTCAACGT GGTGATCGCT GTGCTGATGA AGCACCTGGA GGAAAGCAAC 
AAGGAGGCCA AAGAGGAGGC CGAGCTGGAG GCTGAGCTGG AGCTGGAGAT GAAGACGCTC 
AGCCCACAGC CCCACTCGCC ACTGGGCAGC CCCTTCCTCT GGCCGGGGGT CGAGGGCCCC 
GACAGCCCCG ATAGCCCCAA GCTCAGGGCC CCGCACACCG CGGCCCACAC GGGAGCAGCC 
TCGCGCTTCT CCCTGGAACA CCCCACGGAC AGACAGCTGT TTGACACCAT ATCCCTGCTG 
ATCCAGGGCT CCCTGGAAGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTCC CGCCCGCAGC CTGGGCGGCT CCGACCCACA GATCCCTCTA 
GCTGAGATGG AGGCTCTGTC TCTGACGTCA GAGATTGTGT CTGAACCGTC CCACTCTCTA 
GCTCTGACGG ATGACTCTTT GCCTGATGAC ACTCACACAC TCTTACCTAG TGCCCTGGAC 
AGCAATATGG AGCCCCACCC CGAGGAGATG CCCCTTGCAC TGGGACTGGA CCTGCTGACT 
GTGAGGAAGT CTGGGGTCAG CCGGACACAC TCCCTGCCCA ATGACAGCTA CATGTTCCGG 
GACGGAGGCA CCGCTGAGGG GTCCCTAGGA TGCAGAAGCT GGGGGCTCCC CAAAGCTCAG 
TCAGGCTCTG TCTTATCTGT CCACTCGCAG CCAGCAGACA CCAGCTACAT CCTGCAGCTT 
CCCAAAGACA CACCTCACCT GCTCCAGCCC CACAGCGCTC CCACCTGGGG CACCATTCCC 
AAACTGCCCC CACCAGGCCG CTCCCCCTTG GCTCAGAGGC CACTCAGGCG CCAGGCAGCA 
ATAAGGACTG ATTCTCTGGA CGTTCAGGGC CTGGGCAGCC GGGAAGACCT GCTGTCCGAG 
GTGAGTGGGC CCTCCCCACC TCTGGCCCGG GCCTCCTCCT TCTGGGGCCA CTTGGGCGCC 
CAGATGCAGC AGCATCCCCA CCGCCAGAGC AAGATCTCGA AGCACATGCC CCCGCCAGCC 
CCCCGCCCAG GCTCAGAAAC CACCTGGAGC AAAGACCCAC CCGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGACCGTGGG CAGCCAGGAG 
GAGCCCCCGT CCCCACGGGA CCTGAAGAAG TGTCACAGCG TGGAGGCCCA GAGCTGCCGG 
CGCAGGCCTG CGTCCTGGCT GGATGAACAG AGGAGACACT CCATCGCGGT CAGCTGCCTG 
GACGGCGGCT CCCAACCCAG CCTGGGCCCC GGCCCCTCTA GCCTTGGCGG CCAGCCTCTT 
GGGGGACCTG GAAGCCGGCC CAAGAAAAAA CTCAGCCCAC CTAGTATCTC CATAGACCCC 
CCAGAGAGTC AGGGCCCTCG GCCCCCACCC AGCCCCGGCG TCTGCCTCCG GCGGAGGGCT 
CCGTCCAGCG ACTCCAGGGA CCCCTCGGCC TCCGGCCCCC CTGACAGCAT GGCTGCCTCG 
CCCCCCCCAA AGAAAGACGT GCTGAGTCTC TCTGGTTTAG CCTCTGACCC AGCAGACCTG 
GACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972118.1
CDS	527..7615
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116643.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116643.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAAGGCC CAGTGTAAAC CCTACTACTC AGACTACTCC 
CGATTCCGGC TCCTCGTCCA CCACTTGTGT ACCAGCCACT ATCTGGACCT CTTCATCACG 
GGTGTCATTG GTCTGAATGT GGTCACCATG GCCATGGAGC ACTACCAGCA GCCCCAGATC 
CTGGACGAGG CCTTGAAGAT CTGCAACTAT ATCTTCACCG TCATCTTTGT CTTGGAGTCA 
GTTTTTAAAC TCGTGGCCTT TGGCTTCCGT CGGTTCTTCC AGGACAGGTG GAACCAGCTG 
GACCTGGCCA TCGTGCTGCT GTCCATCATG GGCATCACGC TGGAGGAGAT TGAGGTCAAT 
GCCTCGCTGC CCATCAACCC CACCATCATC CGCATCATGA GAGTGCTGCG CATTGCCCGA 
GTGCTGAAGC TGCTGAAGAT GGCTGTGGGC ATGCGGGCGC TGCTGGACAC AGTCATGCAG 
GCCCTGCCTC AGGTGGGGAA CCTGGGACTT CTCTTCATGT TGTTGTTTTT CATCTTTGCA 
GCTCTGGGTG TGGAGCTCTT TGGAGACCTG GAGTGTGATG AGACGCACCC CTGCGAGGGC 
CTGGGCCGGC ATGCCACCTT TCGGAACTTT GGCATGGCCT TCCTGACCCT CTTCCGAGTG 
TCCACAGGTG ACAACTGGAA CGGCATTATG AAGGACACCC TCCGGGACTG TGACCAGGAG 
TCCACATGCT ACAATACAGT CATCTCCCCC ATCTACTTCG TGTCCTTCGT GCTGACGGCC 
CAGTTTGTGC TGGTCAACGT GGTGATCGCT GTGCTGATGA AGCACCTGGA GGAAAGCAAC 
AAGGAGGCCA AAGAGGAGGC CGAGCTGGAG GCTGAGCTGG AGCTGGAGAT GAAGACGCTC 
AGCCCACAGC CCCACTCGCC ACTGGGCAGC CCCTTCCTCT GGCCGGGGGT CGAGGGCCCC 
GACAGCCCCG ATAGCCCCAA GCTCAGGGCC CCGCACACCG CGGCCCACAC GGGAGCAGCC 
TCGCGCTTCT CCCTGGAACA CCCCACGGAC AGACAGCTGT TTGACACCAT ATCCCTGCTG 
ATCCAGGGCT CCCTGGAAGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTCC CGCCCGCAGC CTGGGCGGCT CCGACCCACA GATCCCTCTA 
GCTGAGATGG AGGCTCTGTC TCTGACGTCA GAGATTGTGT CTGAACCGTC CCACTCTCTA 
GCTCTGACGG ATGACTCTTT GCCTGATGAC ACTCACACAC TCTTACCTAG TGCCCTGGAC 
AGCAATATGG AGCCCCACCC CGAGGAGATG CCCCTTGCAC TGGGACTGGA CCTGCTGACT 
GTGAGGAAGT CTGGGGTCAG CCGGACACAC TCCCTGCCCA ATGACAGCTA CATGTTCCGG 
GACGGAGGCA CCGCTGAGGG GTCCCTAGGA TGCAGAAGCT GGGGGCTCCC CAAAGCTCAG 
TCAGGCTCTG TCTTATCTGT CCACTCGCAG CCAGCAGACA CCAGCTACAT CCTGCAGCTT 
CCCAAAGACA CACCTCACCT GCTCCAGCCC CACAGCGCTC CCACCTGGGG CACCATTCCC 
AAACTGCCCC CACCAGGCCG CTCCCCCTTG GCTCAGAGGC CACTCAGGCG CCAGGCAGCA 
ATAAGGACTG ATTCTCTGGA CGTTCAGGGC CTGGGCAGCC GGGAAGACCT GCTGTCCGAG 
GTGAGTGGGC CCTCCCCACC TCTGGCCCGG GCCTCCTCCT TCTGGGGCCA CTTGGGCGCC 
CAGATGCAGC AGCATCCCCA CCGCCAGAGC AAGATCTCGA AGCACATGCC CCCGCCAGCC 
CCCCGCCCAG GCTCAGAAAC CACCTGGAGC AAAGACCCAC CCGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGACCGTGGG CAGCCAGGAG 
GAGCCCCCGT CCCCACGGGA CCTGAAGAAG TGTCACAGCG TGGAGGCCCA GAGCTGCCGG 
CGCAGGCCTG CGTCCTGGCT GGATGAACAG AGGAGACACT CCATCGCGGT CAGCTGCCTG 
GACGGCGGCT CCCAACCCAG CCTGGGCCCC GGCCCCTCTA GCCTTGGCGG CCAGCCTCTT 
GGGGGACCTG GAAGCCGGCC CAAGAAAAAA CTCAGCCCAC CTAGTATCTC CATAGACCCC 
CCAGAGAGTC AGGGCCCTCG GCCCCCACCC AGCCCCGGCG TCTGCCTCCG GCGGAGGGCT 
CCGTCCAGCG ACTCCAGGGA CCCCTCGGCC TCCGGCCCCC CTGACAGCAT GGCTGCCTCG 
CCCCCCCCAA AGAAAGACGT GCTGAGTCTC TCTGGTTTAG CCTCTGACCC AGCAGACCTG 
GACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116643.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972118.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X9, mRNA.

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CloneID	OCe38392
Clone ID Related Accession (Same CDS sequence)	XM_014116643.1 , XM_014116643.2
Accession Version	XM_014116643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAAGGCC CAGTGTAAAC CCTACTACTC AGACTACTCC 
CGATTCCGGC TCCTCGTCCA CCACTTGTGT ACCAGCCACT ATCTGGACCT CTTCATCACG 
GGTGTCATTG GTCTGAATGT GGTCACCATG GCCATGGAGC ACTACCAGCA GCCCCAGATC 
CTGGACGAGG CCTTGAAGAT CTGCAACTAT ATCTTCACCG TCATCTTTGT CTTGGAGTCA 
GTTTTTAAAC TCGTGGCCTT TGGCTTCCGT CGGTTCTTCC AGGACAGGTG GAACCAGCTG 
GACCTGGCCA TCGTGCTGCT GTCCATCATG GGCATCACGC TGGAGGAGAT TGAGGTCAAT 
GCCTCGCTGC CCATCAACCC CACCATCATC CGCATCATGA GAGTGCTGCG CATTGCCCGA 
GTGCTGAAGC TGCTGAAGAT GGCTGTGGGC ATGCGGGCGC TGCTGGACAC AGTCATGCAG 
GCCCTGCCTC AGGTGGGGAA CCTGGGACTT CTCTTCATGT TGTTGTTTTT CATCTTTGCA 
GCTCTGGGTG TGGAGCTCTT TGGAGACCTG GAGTGTGATG AGACGCACCC CTGCGAGGGC 
CTGGGCCGGC ATGCCACCTT TCGGAACTTT GGCATGGCCT TCCTGACCCT CTTCCGAGTG 
TCCACAGGTG ACAACTGGAA CGGCATTATG AAGGACACCC TCCGGGACTG TGACCAGGAG 
TCCACATGCT ACAATACAGT CATCTCCCCC ATCTACTTCG TGTCCTTCGT GCTGACGGCC 
CAGTTTGTGC TGGTCAACGT GGTGATCGCT GTGCTGATGA AGCACCTGGA GGAAAGCAAC 
AAGGAGGCCA AAGAGGAGGC CGAGCTGGAG GCTGAGCTGG AGCTGGAGAT GAAGACGCTC 
AGCCCACAGC CCCACTCGCC ACTGGGCAGC CCCTTCCTCT GGCCGGGGGT CGAGGGCCCC 
GACAGCCCCG ATAGCCCCAA GCTCAGGGCC CCGCACACCG CGGCCCACAC GGGAGCAGCC 
TCGCGCTTCT CCCTGGAACA CCCCACGGAC AGACAGCTGT TTGACACCAT ATCCCTGCTG 
ATCCAGGGCT CCCTGGAAGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTCC CGCCCGCAGC CTGGGCGGCT CCGACCCACA GATCCCTCTA 
GCTGAGATGG AGGCTCTGTC TCTGACGTCA GAGATTGTGT CTGAACCGTC CCACTCTCTA 
GCTCTGACGG ATGACTCTTT GCCTGATGAC ACTCACACAC TCTTACCTAG TGCCCTGGAC 
AGCAATATGG AGCCCCACCC CGAGGAGATG CCCCTTGCAC TGGGACTGGA CCTGCTGACT 
GTGAGGAAGT CTGGGGTCAG CCGGACACAC TCCCTGCCCA ATGACAGCTA CATGTTCCGG 
GACGGAGGCA CCGCTGAGGG GTCCCTAGGA TGCAGAAGCT GGGGGCTCCC CAAAGCTCAG 
TCAGGCTCTG TCTTATCTGT CCACTCGCAG CCAGCAGACA CCAGCTACAT CCTGCAGCTT 
CCCAAAGACA CACCTCACCT GCTCCAGCCC CACAGCGCTC CCACCTGGGG CACCATTCCC 
AAACTGCCCC CACCAGGCCG CTCCCCCTTG GCTCAGAGGC CACTCAGGCG CCAGGCAGCA 
ATAAGGACTG ATTCTCTGGA CGTTCAGGGC CTGGGCAGCC GGGAAGACCT GCTGTCCGAG 
GTGAGTGGGC CCTCCCCACC TCTGGCCCGG GCCTCCTCCT TCTGGGGCCA CTTGGGCGCC 
CAGATGCAGC AGCATCCCCA CCGCCAGAGC AAGATCTCGA AGCACATGCC CCCGCCAGCC 
CCCCGCCCAG GCTCAGAAAC CACCTGGAGC AAAGACCCAC CCGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGACCGTGGG CAGCCAGGAG 
GAGCCCCCGT CCCCACGGGA CCTGAAGAAG TGTCACAGCG TGGAGGCCCA GAGCTGCCGG 
CGCAGGCCTG CGTCCTGGCT GGATGAACAG AGGAGACACT CCATCGCGGT CAGCTGCCTG 
GACGGCGGCT CCCAACCCAG CCTGGGCCCC GGCCCCTCTA GCCTTGGCGG CCAGCCTCTT 
GGGGGACCTG GAAGCCGGCC CAAGAAAAAA CTCAGCCCAC CTAGTATCTC CATAGACCCC 
CCAGAGAGTC AGGGCCCTCG GCCCCCACCC AGCCCCGGCG TCTGCCTCCG GCGGAGGGCT 
CCGTCCAGCG ACTCCAGGGA CCCCTCGGCC TCCGGCCCCC CTGACAGCAT GGCTGCCTCG 
CCCCCCCCAA AGAAAGACGT GCTGAGTCTC TCTGGTTTAG CCTCTGACCC AGCAGACCTG 
GACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972118.1
CDS	505..7593
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLALVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116643.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116643.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTTGGTGT CCCTGGGAGA GCACCCAGAG CTGCGGAGGA ACCTGCTGCC ACCTCTTATC 
ATCCACACGG CGGCCACACC CATGTCGCTC CCCAAGAGTT CCAGCACTGG CCTGGCCGAG 
GTGCTGGGCC CTGCCTCCCG CCGCACCAGC AGCAGCGGGT CTGCTGAGCC AGGGGCGGCC 
CATGAGATGA AGTCACCGCC GAGCGCCCGC AGCACTCCGC ACAGCCCCTG GAGTGCAGCA 
AGCAGCTGGA CCAGCAGGCG CTCCAGCCGG AACAGCCTGG GCCGAGCCCC CAGCCTGAAG 
CGGAGGAGCC CGAGTGGGGA GCGGCGGTCC CTGTTGTCCG GCGAGGGTCG GGAGAGCCAG 
GATGAGGAGG AGAGCTCTGA AGAGGAGCGG GCCAGCCCAG CGGGCAGCGA CCGGCGCCAT 
GGAGGCTCCC TGGAGCGGGA GGCCAAGAGC TCCTTCGACC TGCCGGACAC GCTGCAAGTG 
CCCGGACTGC ACCGCACGGC CAGTGGCCGC AGCTCAGCCT CTGAGCACCA GGACTGCAAC 
GGCAAGTCGG CCTCGGGGCG CCTGGCCCGG GCCCTGCGGC CCGACGACCC CCCACTGGAT 
GGGGATGATG GCGATGACGA GGGCAACCTG AGCAGAGGGG AGCGGATAAG AGTGTGGATC 
CGAGCCCGGC TGCCTGCCTG CTGCCGCGAG CGTGATTCCT GGTCCGCGTA CATCTTCCCT 
CCTCAGTCCA GGTTCCGCCT CTTATGTCAC CGAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTCCTTG TCATCATCTT CCTCAACTGC ATCACCATCG CCATGGAGCG TCCCAAGATC 
GACCCCCACA GTGCTGAGCG CATCTTCCTG ACCCTCTCCA ATTACATCTT TACCGCAGTC 
TTTCTGGCAG AGATGACCGT GAAGGTGGTA GCGCTGGGCT GGTGCTTCGG GGAGCAGGCG 
TACCTGCGTA GCAGCTGGAA CGTTCTCGAC GGGCTGCTGG TGCTCATCTC CATCATCGAC 
ATCCTGGTGT CCATGGTCTC CGACAGCGGC ACCAAGATCC TGGGCATGTT GAGGGTGCTG 
CGGCTGCTAC GGACCCTGCG CCCACTCAGG GTTATTAGCA GAGCCCAGGG GCTGAAGCTG 
GTAGTGGAAA CGCTGATGTC CTCACTGAAG CCCATCGGCA ACATCGTGGT CATTTGTTGT 
GCCTTCTTCA TCATATTTGG CATCCTGGGG GTGCAGCTCT TCAAAGGGAA GTTCTTCGTG 
TGCCAGGGCG AGGACACCAG GAACATCACC AACAAGTCTG ACTGCACCGA GGCCAGTTAC 
CGGTGGGTCC GGCACAAGTA CAATTTTGAC AACCTCGGCC AGGCCCTGAT GTCCCTGTTC 
GTACTGGCCT CCAAGGACGG CTGGGTGGAC ATCATGTACG ACGGGCTGGA CGCCGTGGGT 
GTGGACCAAC AGCCCATCAT GAACCACAAC CCCTGGATGC TGCTGTACTT CATCTCGTTC 
CTGCTCATTG TGGCCTTCTT TGTCCTGAAC ATGTTTGTGG GTGTGGTGGT GGAGAACTTT 
CACAAGTGTC GGCAGCACCA GGAGGAGGAG GAGGCCCGGC GGCGAGAGGA GAAACGTCTG 
CGGAGACTGG AGAAAAAGAG AAGGAAGGCC CAGTGTAAAC CCTACTACTC AGACTACTCC 
CGATTCCGGC TCCTCGTCCA CCACTTGTGT ACCAGCCACT ATCTGGACCT CTTCATCACG 
GGTGTCATTG GTCTGAATGT GGTCACCATG GCCATGGAGC ACTACCAGCA GCCCCAGATC 
CTGGACGAGG CCTTGAAGAT CTGCAACTAT ATCTTCACCG TCATCTTTGT CTTGGAGTCA 
GTTTTTAAAC TCGTGGCCTT TGGCTTCCGT CGGTTCTTCC AGGACAGGTG GAACCAGCTG 
GACCTGGCCA TCGTGCTGCT GTCCATCATG GGCATCACGC TGGAGGAGAT TGAGGTCAAT 
GCCTCGCTGC CCATCAACCC CACCATCATC CGCATCATGA GAGTGCTGCG CATTGCCCGA 
GTGCTGAAGC TGCTGAAGAT GGCTGTGGGC ATGCGGGCGC TGCTGGACAC AGTCATGCAG 
GCCCTGCCTC AGGTGGGGAA CCTGGGACTT CTCTTCATGT TGTTGTTTTT CATCTTTGCA 
GCTCTGGGTG TGGAGCTCTT TGGAGACCTG GAGTGTGATG AGACGCACCC CTGCGAGGGC 
CTGGGCCGGC ATGCCACCTT TCGGAACTTT GGCATGGCCT TCCTGACCCT CTTCCGAGTG 
TCCACAGGTG ACAACTGGAA CGGCATTATG AAGGACACCC TCCGGGACTG TGACCAGGAG 
TCCACATGCT ACAATACAGT CATCTCCCCC ATCTACTTCG TGTCCTTCGT GCTGACGGCC 
CAGTTTGTGC TGGTCAACGT GGTGATCGCT GTGCTGATGA AGCACCTGGA GGAAAGCAAC 
AAGGAGGCCA AAGAGGAGGC CGAGCTGGAG GCTGAGCTGG AGCTGGAGAT GAAGACGCTC 
AGCCCACAGC CCCACTCGCC ACTGGGCAGC CCCTTCCTCT GGCCGGGGGT CGAGGGCCCC 
GACAGCCCCG ATAGCCCCAA GCTCAGGGCC CCGCACACCG CGGCCCACAC GGGAGCAGCC 
TCGCGCTTCT CCCTGGAACA CCCCACGGAC AGACAGCTGT TTGACACCAT ATCCCTGCTG 
ATCCAGGGCT CCCTGGAAGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTCC CGCCCGCAGC CTGGGCGGCT CCGACCCACA GATCCCTCTA 
GCTGAGATGG AGGCTCTGTC TCTGACGTCA GAGATTGTGT CTGAACCGTC CCACTCTCTA 
GCTCTGACGG ATGACTCTTT GCCTGATGAC ACTCACACAC TCTTACCTAG TGCCCTGGAC 
AGCAATATGG AGCCCCACCC CGAGGAGATG CCCCTTGCAC TGGGACTGGA CCTGCTGACT 
GTGAGGAAGT CTGGGGTCAG CCGGACACAC TCCCTGCCCA ATGACAGCTA CATGTTCCGG 
GACGGAGGCA CCGCTGAGGG GTCCCTAGGA TGCAGAAGCT GGGGGCTCCC CAAAGCTCAG 
TCAGGCTCTG TCTTATCTGT CCACTCGCAG CCAGCAGACA CCAGCTACAT CCTGCAGCTT 
CCCAAAGACA CACCTCACCT GCTCCAGCCC CACAGCGCTC CCACCTGGGG CACCATTCCC 
AAACTGCCCC CACCAGGCCG CTCCCCCTTG GCTCAGAGGC CACTCAGGCG CCAGGCAGCA 
ATAAGGACTG ATTCTCTGGA CGTTCAGGGC CTGGGCAGCC GGGAAGACCT GCTGTCCGAG 
GTGAGTGGGC CCTCCCCACC TCTGGCCCGG GCCTCCTCCT TCTGGGGCCA CTTGGGCGCC 
CAGATGCAGC AGCATCCCCA CCGCCAGAGC AAGATCTCGA AGCACATGCC CCCGCCAGCC 
CCCCGCCCAG GCTCAGAAAC CACCTGGAGC AAAGACCCAC CCGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGACCGTGGG CAGCCAGGAG 
GAGCCCCCGT CCCCACGGGA CCTGAAGAAG TGTCACAGCG TGGAGGCCCA GAGCTGCCGG 
CGCAGGCCTG CGTCCTGGCT GGATGAACAG AGGAGACACT CCATCGCGGT CAGCTGCCTG 
GACGGCGGCT CCCAACCCAG CCTGGGCCCC GGCCCCTCTA GCCTTGGCGG CCAGCCTCTT 
GGGGGACCTG GAAGCCGGCC CAAGAAAAAA CTCAGCCCAC CTAGTATCTC CATAGACCCC 
CCAGAGAGTC AGGGCCCTCG GCCCCCACCC AGCCCCGGCG TCTGCCTCCG GCGGAGGGCT 
CCGTCCAGCG ACTCCAGGGA CCCCTCGGCC TCCGGCCCCC CTGACAGCAT GGCTGCCTCG 
CCCCCCCCAA AGAAAGACGT GCTGAGTCTC TCTGGTTTAG CCTCTGACCC AGCAGACCTG 
GACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116644.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972119.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X14, mRNA.

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CloneID	OCe38393
Clone ID Related Accession (Same CDS sequence)	XM_014116644.1
Accession Version	XM_014116644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGAGGCCCA GTGTAAACCC 
TACTACTCAG ACTACTCCCG ATTCCGGCTC CTCGTCCACC ACTTGTGTAC CAGCCACTAT 
CTGGACCTCT TCATCACGGG TGTCATTGGT CTGAATGTGG TCACCATGGC CATGGAGCAC 
TACCAGCAGC CCCAGATCCT GGACGAGGCC TTGAAGATCT GCAACTATAT CTTCACCGTC 
ATCTTTGTCT TGGAGTCAGT TTTTAAACTC GTGGCCTTTG GCTTCCGTCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCCATC GTGCTGCTGT CCATCATGGG CATCACGCTG 
GAGGAGATTG AGGTCAATGC CTCGCTGCCC ATCAACCCCA CCATCATCCG CATCATGAGA 
GTGCTGCGCA TTGCCCGAGT GCTGAAGCTG CTGAAGATGG CTGTGGGCAT GCGGGCGCTG 
CTGGACACAG TCATGCAGGC CCTGCCTCAG GTGGGGAACC TGGGACTTCT CTTCATGTTG 
TTGTTTTTCA TCTTTGCAGC TCTGGGTGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACGCACCCCT GCGAGGGCCT GGGCCGGCAT GCCACCTTTC GGAACTTTGG CATGGCCTTC 
CTGACCCTCT TCCGAGTGTC CACAGGTGAC AACTGGAACG GCATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACATGCTAC AATACAGTCA TCTCCCCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TGATCGCTGT GCTGATGAAG 
CACCTGGAGG AAAGCAACAA GGAGGCCAAA GAGGAGGCCG AGCTGGAGGC TGAGCTGGAG 
CTGGAGATGA AGACGCTCAG CCCACAGCCC CACTCGCCAC TGGGCAGCCC CTTCCTCTGG 
CCGGGGGTCG AGGGCCCCGA CAGCCCCGAT AGCCCCAAGC TCAGGGCCCC GCACACCGCG 
GCCCACACGG GAGCAGCCTC GCGCTTCTCC CTGGAACACC CCACGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTCTGAC GTCAGAGATT GTGTCTGAAC CGTCCCACTC TCTAGCTCTG 
ACGGATGACT CTTTGCCTGA TGACACTCAC ACACTCTTAC CTAGTGCCCT GGACAGCAAT 
ATGGAGCCCC ACCCCGAGGA GATGCCCCTT GCACTGGGAC TGGACCTGCT GACTGTGAGG 
AAGTCTGGGG TCAGCCGGAC ACACTCCCTG CCCAATGACA GCTACATGTT CCGGGACGGA 
GGCACCGCTG AGGGGTCCCT AGGATGCAGA AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC 
TCTGTCTTAT CTGTCCACTC GCAGCCAGCA GACACCAGCT ACATCCTGCA GCTTCCCAAA 
GACACACCTC ACCTGCTCCA GCCCCACAGC GCTCCCACCT GGGGCACCAT TCCCAAACTG 
CCCCCACCAG GCCGCTCCCC CTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATAAGG 
ACTGATTCTC TGGACGTTCA GGGCCTGGGC AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT 
GGGCCCTCCC CACCTCTGGC CCGGGCCTCC TCCTTCTGGG GCCACTTGGG CGCCCAGATG 
CAGCAGCATC CCCACCGCCA GAGCAAGATC TCGAAGCACA TGCCCCCGCC AGCCCCCCGC 
CCAGGCTCAG AAACCACCTG GAGCAAAGAC CCACCCGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGACCG TGGGCAGCCA GGAGGAGCCC 
CCGTCCCCAC GGGACCTGAA GAAGTGTCAC AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG 
CCTGCGTCCT GGCTGGATGA ACAGAGGAGA CACTCCATCG CGGTCAGCTG CCTGGACGGC 
GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA 
CCTGGAAGCC GGCCCAAGAA AAAACTCAGC CCACCTAGTA TCTCCATAGA CCCCCCAGAG 
AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC 
AGCGACTCCA GGGACCCCTC GGCCTCCGGC CCCCCTGACA GCATGGCTGC CTCGCCCCCC 
CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTAGCCTCTG ACCCAGCAGA CCTGGACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972119.1
CDS	527..7549
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMADLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTIPLAEMEALSLTSEIVSEPSHSLALTDDSLPDDTHTLLPSALDSNMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116644.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116644.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA TCTAATGCTG 
GACGATGTAA TTGCTTCCGG CAGCTCATCC AGCGCGGCGC CAGAGGCCCA GTGTAAACCC 
TACTACTCAG ACTACTCCCG ATTCCGGCTC CTCGTCCACC ACTTGTGTAC CAGCCACTAT 
CTGGACCTCT TCATCACGGG TGTCATTGGT CTGAATGTGG TCACCATGGC CATGGAGCAC 
TACCAGCAGC CCCAGATCCT GGACGAGGCC TTGAAGATCT GCAACTATAT CTTCACCGTC 
ATCTTTGTCT TGGAGTCAGT TTTTAAACTC GTGGCCTTTG GCTTCCGTCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCCATC GTGCTGCTGT CCATCATGGG CATCACGCTG 
GAGGAGATTG AGGTCAATGC CTCGCTGCCC ATCAACCCCA CCATCATCCG CATCATGAGA 
GTGCTGCGCA TTGCCCGAGT GCTGAAGCTG CTGAAGATGG CTGTGGGCAT GCGGGCGCTG 
CTGGACACAG TCATGCAGGC CCTGCCTCAG GTGGGGAACC TGGGACTTCT CTTCATGTTG 
TTGTTTTTCA TCTTTGCAGC TCTGGGTGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACGCACCCCT GCGAGGGCCT GGGCCGGCAT GCCACCTTTC GGAACTTTGG CATGGCCTTC 
CTGACCCTCT TCCGAGTGTC CACAGGTGAC AACTGGAACG GCATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACATGCTAC AATACAGTCA TCTCCCCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TGATCGCTGT GCTGATGAAG 
CACCTGGAGG AAAGCAACAA GGAGGCCAAA GAGGAGGCCG AGCTGGAGGC TGAGCTGGAG 
CTGGAGATGA AGACGCTCAG CCCACAGCCC CACTCGCCAC TGGGCAGCCC CTTCCTCTGG 
CCGGGGGTCG AGGGCCCCGA CAGCCCCGAT AGCCCCAAGC TCAGGGCCCC GCACACCGCG 
GCCCACACGG GAGCAGCCTC GCGCTTCTCC CTGGAACACC CCACGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTCTGAC GTCAGAGATT GTGTCTGAAC CGTCCCACTC TCTAGCTCTG 
ACGGATGACT CTTTGCCTGA TGACACTCAC ACACTCTTAC CTAGTGCCCT GGACAGCAAT 
ATGGAGCCCC ACCCCGAGGA GATGCCCCTT GCACTGGGAC TGGACCTGCT GACTGTGAGG 
AAGTCTGGGG TCAGCCGGAC ACACTCCCTG CCCAATGACA GCTACATGTT CCGGGACGGA 
GGCACCGCTG AGGGGTCCCT AGGATGCAGA AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC 
TCTGTCTTAT CTGTCCACTC GCAGCCAGCA GACACCAGCT ACATCCTGCA GCTTCCCAAA 
GACACACCTC ACCTGCTCCA GCCCCACAGC GCTCCCACCT GGGGCACCAT TCCCAAACTG 
CCCCCACCAG GCCGCTCCCC CTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATAAGG 
ACTGATTCTC TGGACGTTCA GGGCCTGGGC AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT 
GGGCCCTCCC CACCTCTGGC CCGGGCCTCC TCCTTCTGGG GCCACTTGGG CGCCCAGATG 
CAGCAGCATC CCCACCGCCA GAGCAAGATC TCGAAGCACA TGCCCCCGCC AGCCCCCCGC 
CCAGGCTCAG AAACCACCTG GAGCAAAGAC CCACCCGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGACCG TGGGCAGCCA GGAGGAGCCC 
CCGTCCCCAC GGGACCTGAA GAAGTGTCAC AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG 
CCTGCGTCCT GGCTGGATGA ACAGAGGAGA CACTCCATCG CGGTCAGCTG CCTGGACGGC 
GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA 
CCTGGAAGCC GGCCCAAGAA AAAACTCAGC CCACCTAGTA TCTCCATAGA CCCCCCAGAG 
AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC 
AGCGACTCCA GGGACCCCTC GGCCTCCGGC CCCCCTGACA GCATGGCTGC CTCGCCCCCC 
CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTAGCCTCTG ACCCAGCAGA CCTGGACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116645.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972120.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X16, mRNA.

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CloneID	OCe38394
Clone ID Related Accession (Same CDS sequence)	XM_014116645.1 , XM_014116645.2
Accession Version	XM_014116645.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA GGCCCAGTGT 
AAACCCTACT ACTCAGACTA CTCCCGATTC CGGCTCCTCG TCCACCACTT GTGTACCAGC 
CACTATCTGG ACCTCTTCAT CACGGGTGTC ATTGGTCTGA ATGTGGTCAC CATGGCCATG 
GAGCACTACC AGCAGCCCCA GATCCTGGAC GAGGCCTTGA AGATCTGCAA CTATATCTTC 
ACCGTCATCT TTGTCTTGGA GTCAGTTTTT AAACTCGTGG CCTTTGGCTT CCGTCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCCATCGTGC TGCTGTCCAT CATGGGCATC 
ACGCTGGAGG AGATTGAGGT CAATGCCTCG CTGCCCATCA ACCCCACCAT CATCCGCATC 
ATGAGAGTGC TGCGCATTGC CCGAGTGCTG AAGCTGCTGA AGATGGCTGT GGGCATGCGG 
GCGCTGCTGG ACACAGTCAT GCAGGCCCTG CCTCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGTTGTTGT TTTTCATCTT TGCAGCTCTG GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACGC ACCCCTGCGA GGGCCTGGGC CGGCATGCCA CCTTTCGGAA CTTTGGCATG 
GCCTTCCTGA CCCTCTTCCG AGTGTCCACA GGTGACAACT GGAACGGCAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACA TGCTACAATA CAGTCATCTC CCCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG 
ATGAAGCACC TGGAGGAAAG CAACAAGGAG GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG 
CTGGAGCTGG AGATGAAGAC GCTCAGCCCA CAGCCCCACT CGCCACTGGG CAGCCCCTTC 
CTCTGGCCGG GGGTCGAGGG CCCCGACAGC CCCGATAGCC CCAAGCTCAG GGCCCCGCAC 
ACCGCGGCCC ACACGGGAGC AGCCTCGCGC TTCTCCCTGG AACACCCCAC GGACAGACAG 
CTGTTTGACA CCATATCCCT GCTGATCCAG GGCTCCCTGG AAGGGGAGCT GAAGCTGATG 
GACGAGCTGG CAGGCCCAGG GGGCCAGCCC TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC 
GGCTCCGACC CACAGATGGA GCCCCACCCC GAGGAGATGC CCCTTGCACT GGGACTGGAC 
CTGCTGACTG TGAGGAAGTC TGGGGTCAGC CGGACACACT CCCTGCCCAA TGACAGCTAC 
ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC 
AAAGCTCAGT CAGGCTCTGT CTTATCTGTC CACTCGCAGC CAGCAGACAC CAGCTACATC 
CTGCAGCTTC CCAAAGACAC ACCTCACCTG CTCCAGCCCC ACAGCGCTCC CACCTGGGGC 
ACCATTCCCA AACTGCCCCC ACCAGGCCGC TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC 
CAGGCAGCAA TAAGGACTGA TTCTCTGGAC GTTCAGGGCC TGGGCAGCCG GGAAGACCTG 
CTGTCCGAGG TGAGTGGGCC CTCCCCACCT CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC 
TTGGGCGCCC AGATGCAGCA GCATCCCCAC CGCCAGAGCA AGATCTCGAA GCACATGCCC 
CCGCCAGCCC CCCGCCCAGG CTCAGAAACC ACCTGGAGCA AAGACCCACC CGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GACCGTGGGC 
AGCCAGGAGG AGCCCCCGTC CCCACGGGAC CTGAAGAAGT GTCACAGCGT GGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG GATGAACAGA GGAGACACTC CATCGCGGTC 
AGCTGCCTGG ACGGCGGCTC CCAACCCAGC CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC 
CAGCCTCTTG GGGGACCTGG AAGCCGGCCC AAGAAAAAAC TCAGCCCACC TAGTATCTCC 
ATAGACCCCC CAGAGAGTCA GGGCCCTCGG CCCCCACCCA GCCCCGGCGT CTGCCTCCGG 
CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC CCCTCGGCCT CCGGCCCCCC TGACAGCATG 
GCTGCCTCGC CCCCCCCAAA GAAAGACGTG CTGAGTCTCT CTGGTTTAGC CTCTGACCCA 
GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972120.1
CDS	527..7504
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116645.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116645.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA GGCCCAGTGT 
AAACCCTACT ACTCAGACTA CTCCCGATTC CGGCTCCTCG TCCACCACTT GTGTACCAGC 
CACTATCTGG ACCTCTTCAT CACGGGTGTC ATTGGTCTGA ATGTGGTCAC CATGGCCATG 
GAGCACTACC AGCAGCCCCA GATCCTGGAC GAGGCCTTGA AGATCTGCAA CTATATCTTC 
ACCGTCATCT TTGTCTTGGA GTCAGTTTTT AAACTCGTGG CCTTTGGCTT CCGTCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCCATCGTGC TGCTGTCCAT CATGGGCATC 
ACGCTGGAGG AGATTGAGGT CAATGCCTCG CTGCCCATCA ACCCCACCAT CATCCGCATC 
ATGAGAGTGC TGCGCATTGC CCGAGTGCTG AAGCTGCTGA AGATGGCTGT GGGCATGCGG 
GCGCTGCTGG ACACAGTCAT GCAGGCCCTG CCTCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGTTGTTGT TTTTCATCTT TGCAGCTCTG GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACGC ACCCCTGCGA GGGCCTGGGC CGGCATGCCA CCTTTCGGAA CTTTGGCATG 
GCCTTCCTGA CCCTCTTCCG AGTGTCCACA GGTGACAACT GGAACGGCAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACA TGCTACAATA CAGTCATCTC CCCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG 
ATGAAGCACC TGGAGGAAAG CAACAAGGAG GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG 
CTGGAGCTGG AGATGAAGAC GCTCAGCCCA CAGCCCCACT CGCCACTGGG CAGCCCCTTC 
CTCTGGCCGG GGGTCGAGGG CCCCGACAGC CCCGATAGCC CCAAGCTCAG GGCCCCGCAC 
ACCGCGGCCC ACACGGGAGC AGCCTCGCGC TTCTCCCTGG AACACCCCAC GGACAGACAG 
CTGTTTGACA CCATATCCCT GCTGATCCAG GGCTCCCTGG AAGGGGAGCT GAAGCTGATG 
GACGAGCTGG CAGGCCCAGG GGGCCAGCCC TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC 
GGCTCCGACC CACAGATGGA GCCCCACCCC GAGGAGATGC CCCTTGCACT GGGACTGGAC 
CTGCTGACTG TGAGGAAGTC TGGGGTCAGC CGGACACACT CCCTGCCCAA TGACAGCTAC 
ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC 
AAAGCTCAGT CAGGCTCTGT CTTATCTGTC CACTCGCAGC CAGCAGACAC CAGCTACATC 
CTGCAGCTTC CCAAAGACAC ACCTCACCTG CTCCAGCCCC ACAGCGCTCC CACCTGGGGC 
ACCATTCCCA AACTGCCCCC ACCAGGCCGC TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC 
CAGGCAGCAA TAAGGACTGA TTCTCTGGAC GTTCAGGGCC TGGGCAGCCG GGAAGACCTG 
CTGTCCGAGG TGAGTGGGCC CTCCCCACCT CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC 
TTGGGCGCCC AGATGCAGCA GCATCCCCAC CGCCAGAGCA AGATCTCGAA GCACATGCCC 
CCGCCAGCCC CCCGCCCAGG CTCAGAAACC ACCTGGAGCA AAGACCCACC CGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GACCGTGGGC 
AGCCAGGAGG AGCCCCCGTC CCCACGGGAC CTGAAGAAGT GTCACAGCGT GGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG GATGAACAGA GGAGACACTC CATCGCGGTC 
AGCTGCCTGG ACGGCGGCTC CCAACCCAGC CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC 
CAGCCTCTTG GGGGACCTGG AAGCCGGCCC AAGAAAAAAC TCAGCCCACC TAGTATCTCC 
ATAGACCCCC CAGAGAGTCA GGGCCCTCGG CCCCCACCCA GCCCCGGCGT CTGCCTCCGG 
CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC CCCTCGGCCT CCGGCCCCCC TGACAGCATG 
GCTGCCTCGC CCCCCCCAAA GAAAGACGTG CTGAGTCTCT CTGGTTTAGC CTCTGACCCA 
GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116645.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972120.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X13, mRNA.

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CloneID	OCe38394
Clone ID Related Accession (Same CDS sequence)	XM_014116645.1 , XM_014116645.2
Accession Version	XM_014116645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA GGCCCAGTGT 
AAACCCTACT ACTCAGACTA CTCCCGATTC CGGCTCCTCG TCCACCACTT GTGTACCAGC 
CACTATCTGG ACCTCTTCAT CACGGGTGTC ATTGGTCTGA ATGTGGTCAC CATGGCCATG 
GAGCACTACC AGCAGCCCCA GATCCTGGAC GAGGCCTTGA AGATCTGCAA CTATATCTTC 
ACCGTCATCT TTGTCTTGGA GTCAGTTTTT AAACTCGTGG CCTTTGGCTT CCGTCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCCATCGTGC TGCTGTCCAT CATGGGCATC 
ACGCTGGAGG AGATTGAGGT CAATGCCTCG CTGCCCATCA ACCCCACCAT CATCCGCATC 
ATGAGAGTGC TGCGCATTGC CCGAGTGCTG AAGCTGCTGA AGATGGCTGT GGGCATGCGG 
GCGCTGCTGG ACACAGTCAT GCAGGCCCTG CCTCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGTTGTTGT TTTTCATCTT TGCAGCTCTG GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACGC ACCCCTGCGA GGGCCTGGGC CGGCATGCCA CCTTTCGGAA CTTTGGCATG 
GCCTTCCTGA CCCTCTTCCG AGTGTCCACA GGTGACAACT GGAACGGCAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACA TGCTACAATA CAGTCATCTC CCCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG 
ATGAAGCACC TGGAGGAAAG CAACAAGGAG GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG 
CTGGAGCTGG AGATGAAGAC GCTCAGCCCA CAGCCCCACT CGCCACTGGG CAGCCCCTTC 
CTCTGGCCGG GGGTCGAGGG CCCCGACAGC CCCGATAGCC CCAAGCTCAG GGCCCCGCAC 
ACCGCGGCCC ACACGGGAGC AGCCTCGCGC TTCTCCCTGG AACACCCCAC GGACAGACAG 
CTGTTTGACA CCATATCCCT GCTGATCCAG GGCTCCCTGG AAGGGGAGCT GAAGCTGATG 
GACGAGCTGG CAGGCCCAGG GGGCCAGCCC TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC 
GGCTCCGACC CACAGATGGA GCCCCACCCC GAGGAGATGC CCCTTGCACT GGGACTGGAC 
CTGCTGACTG TGAGGAAGTC TGGGGTCAGC CGGACACACT CCCTGCCCAA TGACAGCTAC 
ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC 
AAAGCTCAGT CAGGCTCTGT CTTATCTGTC CACTCGCAGC CAGCAGACAC CAGCTACATC 
CTGCAGCTTC CCAAAGACAC ACCTCACCTG CTCCAGCCCC ACAGCGCTCC CACCTGGGGC 
ACCATTCCCA AACTGCCCCC ACCAGGCCGC TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC 
CAGGCAGCAA TAAGGACTGA TTCTCTGGAC GTTCAGGGCC TGGGCAGCCG GGAAGACCTG 
CTGTCCGAGG TGAGTGGGCC CTCCCCACCT CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC 
TTGGGCGCCC AGATGCAGCA GCATCCCCAC CGCCAGAGCA AGATCTCGAA GCACATGCCC 
CCGCCAGCCC CCCGCCCAGG CTCAGAAACC ACCTGGAGCA AAGACCCACC CGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GACCGTGGGC 
AGCCAGGAGG AGCCCCCGTC CCCACGGGAC CTGAAGAAGT GTCACAGCGT GGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG GATGAACAGA GGAGACACTC CATCGCGGTC 
AGCTGCCTGG ACGGCGGCTC CCAACCCAGC CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC 
CAGCCTCTTG GGGGACCTGG AAGCCGGCCC AAGAAAAAAC TCAGCCCACC TAGTATCTCC 
ATAGACCCCC CAGAGAGTCA GGGCCCTCGG CCCCCACCCA GCCCCGGCGT CTGCCTCCGG 
CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC CCCTCGGCCT CCGGCCCCCC TGACAGCATG 
GCTGCCTCGC CCCCCCCAAA GAAAGACGTG CTGAGTCTCT CTGGTTTAGC CTCTGACCCA 
GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972120.1
CDS	504..7481
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPPARSLGGSDPQMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116645.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116645.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA GGCCCAGTGT 
AAACCCTACT ACTCAGACTA CTCCCGATTC CGGCTCCTCG TCCACCACTT GTGTACCAGC 
CACTATCTGG ACCTCTTCAT CACGGGTGTC ATTGGTCTGA ATGTGGTCAC CATGGCCATG 
GAGCACTACC AGCAGCCCCA GATCCTGGAC GAGGCCTTGA AGATCTGCAA CTATATCTTC 
ACCGTCATCT TTGTCTTGGA GTCAGTTTTT AAACTCGTGG CCTTTGGCTT CCGTCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCCATCGTGC TGCTGTCCAT CATGGGCATC 
ACGCTGGAGG AGATTGAGGT CAATGCCTCG CTGCCCATCA ACCCCACCAT CATCCGCATC 
ATGAGAGTGC TGCGCATTGC CCGAGTGCTG AAGCTGCTGA AGATGGCTGT GGGCATGCGG 
GCGCTGCTGG ACACAGTCAT GCAGGCCCTG CCTCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGTTGTTGT TTTTCATCTT TGCAGCTCTG GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACGC ACCCCTGCGA GGGCCTGGGC CGGCATGCCA CCTTTCGGAA CTTTGGCATG 
GCCTTCCTGA CCCTCTTCCG AGTGTCCACA GGTGACAACT GGAACGGCAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACA TGCTACAATA CAGTCATCTC CCCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG 
ATGAAGCACC TGGAGGAAAG CAACAAGGAG GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG 
CTGGAGCTGG AGATGAAGAC GCTCAGCCCA CAGCCCCACT CGCCACTGGG CAGCCCCTTC 
CTCTGGCCGG GGGTCGAGGG CCCCGACAGC CCCGATAGCC CCAAGCTCAG GGCCCCGCAC 
ACCGCGGCCC ACACGGGAGC AGCCTCGCGC TTCTCCCTGG AACACCCCAC GGACAGACAG 
CTGTTTGACA CCATATCCCT GCTGATCCAG GGCTCCCTGG AAGGGGAGCT GAAGCTGATG 
GACGAGCTGG CAGGCCCAGG GGGCCAGCCC TCTGCCTTCC CTCCCGCCCG CAGCCTGGGC 
GGCTCCGACC CACAGATGGA GCCCCACCCC GAGGAGATGC CCCTTGCACT GGGACTGGAC 
CTGCTGACTG TGAGGAAGTC TGGGGTCAGC CGGACACACT CCCTGCCCAA TGACAGCTAC 
ATGTTCCGGG ACGGAGGCAC CGCTGAGGGG TCCCTAGGAT GCAGAAGCTG GGGGCTCCCC 
AAAGCTCAGT CAGGCTCTGT CTTATCTGTC CACTCGCAGC CAGCAGACAC CAGCTACATC 
CTGCAGCTTC CCAAAGACAC ACCTCACCTG CTCCAGCCCC ACAGCGCTCC CACCTGGGGC 
ACCATTCCCA AACTGCCCCC ACCAGGCCGC TCCCCCTTGG CTCAGAGGCC ACTCAGGCGC 
CAGGCAGCAA TAAGGACTGA TTCTCTGGAC GTTCAGGGCC TGGGCAGCCG GGAAGACCTG 
CTGTCCGAGG TGAGTGGGCC CTCCCCACCT CTGGCCCGGG CCTCCTCCTT CTGGGGCCAC 
TTGGGCGCCC AGATGCAGCA GCATCCCCAC CGCCAGAGCA AGATCTCGAA GCACATGCCC 
CCGCCAGCCC CCCGCCCAGG CTCAGAAACC ACCTGGAGCA AAGACCCACC CGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GACCGTGGGC 
AGCCAGGAGG AGCCCCCGTC CCCACGGGAC CTGAAGAAGT GTCACAGCGT GGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGC GTCCTGGCTG GATGAACAGA GGAGACACTC CATCGCGGTC 
AGCTGCCTGG ACGGCGGCTC CCAACCCAGC CTGGGCCCCG GCCCCTCTAG CCTTGGCGGC 
CAGCCTCTTG GGGGACCTGG AAGCCGGCCC AAGAAAAAAC TCAGCCCACC TAGTATCTCC 
ATAGACCCCC CAGAGAGTCA GGGCCCTCGG CCCCCACCCA GCCCCGGCGT CTGCCTCCGG 
CGGAGGGCTC CGTCCAGCGA CTCCAGGGAC CCCTCGGCCT CCGGCCCCCC TGACAGCATG 
GCTGCCTCGC CCCCCCCAAA GAAAGACGTG CTGAGTCTCT CTGGTTTAGC CTCTGACCCA 
GCAGACCTGG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116647.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972122.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X23, mRNA.

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CloneID	OCe38395
Clone ID Related Accession (Same CDS sequence)	XM_014116647.1 , XM_014116647.2
Accession Version	XM_014116647.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X23
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAT CTAATGCTGG ACGATGTAAT TGCTTCCGGC 
AGCTCATCCA GCGCGGCGCC AGAGGCCCAG TGTAAACCCT ACTACTCAGA CTACTCCCGA 
TTCCGGCTCC TCGTCCACCA CTTGTGTACC AGCCACTATC TGGACCTCTT CATCACGGGT 
GTCATTGGTC TGAATGTGGT CACCATGGCC ATGGAGCACT ACCAGCAGCC CCAGATCCTG 
GACGAGGCCT TGAAGATCTG CAACTATATC TTCACCGTCA TCTTTGTCTT GGAGTCAGTT 
TTTAAACTCG TGGCCTTTGG CTTCCGTCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTGCTGTC CATCATGGGC ATCACGCTGG AGGAGATTGA GGTCAATGCC 
TCGCTGCCCA TCAACCCCAC CATCATCCGC ATCATGAGAG TGCTGCGCAT TGCCCGAGTG 
CTGAAGCTGC TGAAGATGGC TGTGGGCATG CGGGCGCTGC TGGACACAGT CATGCAGGCC 
CTGCCTCAGG TGGGGAACCT GGGACTTCTC TTCATGTTGT TGTTTTTCAT CTTTGCAGCT 
CTGGGTGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CGCACCCCTG CGAGGGCCTG 
GGCCGGCATG CCACCTTTCG GAACTTTGGC ATGGCCTTCC TGACCCTCTT CCGAGTGTCC 
ACAGGTGACA ACTGGAACGG CATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACATGCTACA ATACAGTCAT CTCCCCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT GATCGCTGTG CTGATGAAGC ACCTGGAGGA AAGCAACAAG 
GAGGCCAAAG AGGAGGCCGA GCTGGAGGCT GAGCTGGAGC TGGAGATGAA GACGCTCAGC 
CCACAGCCCC ACTCGCCACT GGGCAGCCCC TTCCTCTGGC CGGGGGTCGA GGGCCCCGAC 
AGCCCCGATA GCCCCAAGCT CAGGGCCCCG CACACCGCGG CCCACACGGG AGCAGCCTCG 
CGCTTCTCCC TGGAACACCC CACGATGGAG CCCCACCCCG AGGAGATGCC CCTTGCACTG 
GGACTGGACC TGCTGACTGT GAGGAAGTCT GGGGTCAGCC GGACACACTC CCTGCCCAAT 
GACAGCTACA TGTTCCGGGA CGGAGGCACC GCTGAGGGGT CCCTAGGATG CAGAAGCTGG 
GGGCTCCCCA AAGCTCAGTC AGGCTCTGTC TTATCTGTCC ACTCGCAGCC AGCAGACACC 
AGCTACATCC TGCAGCTTCC CAAAGACACA CCTCACCTGC TCCAGCCCCA CAGCGCTCCC 
ACCTGGGGCA CCATTCCCAA ACTGCCCCCA CCAGGCCGCT CCCCCTTGGC TCAGAGGCCA 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAT TCTCTGGACG TTCAGGGCCT GGGCAGCCGG 
GAAGACCTGC TGTCCGAGGT GAGTGGGCCC TCCCCACCTC TGGCCCGGGC CTCCTCCTTC 
TGGGGCCACT TGGGCGCCCA GATGCAGCAG CATCCCCACC GCCAGAGCAA GATCTCGAAG 
CACATGCCCC CGCCAGCCCC CCGCCCAGGC TCAGAAACCA CCTGGAGCAA AGACCCACCC 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
ACCGTGGGCA GCCAGGAGGA GCCCCCGTCC CCACGGGACC TGAAGAAGTG TCACAGCGTG 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGCG TCCTGGCTGG ATGAACAGAG GAGACACTCC 
ATCGCGGTCA GCTGCCTGGA CGGCGGCTCC CAACCCAGCC TGGGCCCCGG CCCCTCTAGC 
CTTGGCGGCC AGCCTCTTGG GGGACCTGGA AGCCGGCCCA AGAAAAAACT CAGCCCACCT 
AGTATCTCCA TAGACCCCCC AGAGAGTCAG GGCCCTCGGC CCCCACCCAG CCCCGGCGTC 
TGCCTCCGGC GGAGGGCTCC GTCCAGCGAC TCCAGGGACC CCTCGGCCTC CGGCCCCCCT 
GACAGCATGG CTGCCTCGCC CCCCCCAAAG AAAGACGTGC TGAGTCTCTC TGGTTTAGCC 
TCTGACCCAG CAGACCTGGA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972122.1
CDS	527..7393
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116647.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116647.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAT CTAATGCTGG ACGATGTAAT TGCTTCCGGC 
AGCTCATCCA GCGCGGCGCC AGAGGCCCAG TGTAAACCCT ACTACTCAGA CTACTCCCGA 
TTCCGGCTCC TCGTCCACCA CTTGTGTACC AGCCACTATC TGGACCTCTT CATCACGGGT 
GTCATTGGTC TGAATGTGGT CACCATGGCC ATGGAGCACT ACCAGCAGCC CCAGATCCTG 
GACGAGGCCT TGAAGATCTG CAACTATATC TTCACCGTCA TCTTTGTCTT GGAGTCAGTT 
TTTAAACTCG TGGCCTTTGG CTTCCGTCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTGCTGTC CATCATGGGC ATCACGCTGG AGGAGATTGA GGTCAATGCC 
TCGCTGCCCA TCAACCCCAC CATCATCCGC ATCATGAGAG TGCTGCGCAT TGCCCGAGTG 
CTGAAGCTGC TGAAGATGGC TGTGGGCATG CGGGCGCTGC TGGACACAGT CATGCAGGCC 
CTGCCTCAGG TGGGGAACCT GGGACTTCTC TTCATGTTGT TGTTTTTCAT CTTTGCAGCT 
CTGGGTGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CGCACCCCTG CGAGGGCCTG 
GGCCGGCATG CCACCTTTCG GAACTTTGGC ATGGCCTTCC TGACCCTCTT CCGAGTGTCC 
ACAGGTGACA ACTGGAACGG CATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACATGCTACA ATACAGTCAT CTCCCCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT GATCGCTGTG CTGATGAAGC ACCTGGAGGA AAGCAACAAG 
GAGGCCAAAG AGGAGGCCGA GCTGGAGGCT GAGCTGGAGC TGGAGATGAA GACGCTCAGC 
CCACAGCCCC ACTCGCCACT GGGCAGCCCC TTCCTCTGGC CGGGGGTCGA GGGCCCCGAC 
AGCCCCGATA GCCCCAAGCT CAGGGCCCCG CACACCGCGG CCCACACGGG AGCAGCCTCG 
CGCTTCTCCC TGGAACACCC CACGATGGAG CCCCACCCCG AGGAGATGCC CCTTGCACTG 
GGACTGGACC TGCTGACTGT GAGGAAGTCT GGGGTCAGCC GGACACACTC CCTGCCCAAT 
GACAGCTACA TGTTCCGGGA CGGAGGCACC GCTGAGGGGT CCCTAGGATG CAGAAGCTGG 
GGGCTCCCCA AAGCTCAGTC AGGCTCTGTC TTATCTGTCC ACTCGCAGCC AGCAGACACC 
AGCTACATCC TGCAGCTTCC CAAAGACACA CCTCACCTGC TCCAGCCCCA CAGCGCTCCC 
ACCTGGGGCA CCATTCCCAA ACTGCCCCCA CCAGGCCGCT CCCCCTTGGC TCAGAGGCCA 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAT TCTCTGGACG TTCAGGGCCT GGGCAGCCGG 
GAAGACCTGC TGTCCGAGGT GAGTGGGCCC TCCCCACCTC TGGCCCGGGC CTCCTCCTTC 
TGGGGCCACT TGGGCGCCCA GATGCAGCAG CATCCCCACC GCCAGAGCAA GATCTCGAAG 
CACATGCCCC CGCCAGCCCC CCGCCCAGGC TCAGAAACCA CCTGGAGCAA AGACCCACCC 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
ACCGTGGGCA GCCAGGAGGA GCCCCCGTCC CCACGGGACC TGAAGAAGTG TCACAGCGTG 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGCG TCCTGGCTGG ATGAACAGAG GAGACACTCC 
ATCGCGGTCA GCTGCCTGGA CGGCGGCTCC CAACCCAGCC TGGGCCCCGG CCCCTCTAGC 
CTTGGCGGCC AGCCTCTTGG GGGACCTGGA AGCCGGCCCA AGAAAAAACT CAGCCCACCT 
AGTATCTCCA TAGACCCCCC AGAGAGTCAG GGCCCTCGGC CCCCACCCAG CCCCGGCGTC 
TGCCTCCGGC GGAGGGCTCC GTCCAGCGAC TCCAGGGACC CCTCGGCCTC CGGCCCCCCT 
GACAGCATGG CTGCCTCGCC CCCCCCAAAG AAAGACGTGC TGAGTCTCTC TGGTTTAGCC 
TCTGACCCAG CAGACCTGGA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116647.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972122.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X19, mRNA.

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CloneID	OCe38395
Clone ID Related Accession (Same CDS sequence)	XM_014116647.1 , XM_014116647.2
Accession Version	XM_014116647.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116647.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAT CTAATGCTGG ACGATGTAAT TGCTTCCGGC 
AGCTCATCCA GCGCGGCGCC AGAGGCCCAG TGTAAACCCT ACTACTCAGA CTACTCCCGA 
TTCCGGCTCC TCGTCCACCA CTTGTGTACC AGCCACTATC TGGACCTCTT CATCACGGGT 
GTCATTGGTC TGAATGTGGT CACCATGGCC ATGGAGCACT ACCAGCAGCC CCAGATCCTG 
GACGAGGCCT TGAAGATCTG CAACTATATC TTCACCGTCA TCTTTGTCTT GGAGTCAGTT 
TTTAAACTCG TGGCCTTTGG CTTCCGTCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTGCTGTC CATCATGGGC ATCACGCTGG AGGAGATTGA GGTCAATGCC 
TCGCTGCCCA TCAACCCCAC CATCATCCGC ATCATGAGAG TGCTGCGCAT TGCCCGAGTG 
CTGAAGCTGC TGAAGATGGC TGTGGGCATG CGGGCGCTGC TGGACACAGT CATGCAGGCC 
CTGCCTCAGG TGGGGAACCT GGGACTTCTC TTCATGTTGT TGTTTTTCAT CTTTGCAGCT 
CTGGGTGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CGCACCCCTG CGAGGGCCTG 
GGCCGGCATG CCACCTTTCG GAACTTTGGC ATGGCCTTCC TGACCCTCTT CCGAGTGTCC 
ACAGGTGACA ACTGGAACGG CATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACATGCTACA ATACAGTCAT CTCCCCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT GATCGCTGTG CTGATGAAGC ACCTGGAGGA AAGCAACAAG 
GAGGCCAAAG AGGAGGCCGA GCTGGAGGCT GAGCTGGAGC TGGAGATGAA GACGCTCAGC 
CCACAGCCCC ACTCGCCACT GGGCAGCCCC TTCCTCTGGC CGGGGGTCGA GGGCCCCGAC 
AGCCCCGATA GCCCCAAGCT CAGGGCCCCG CACACCGCGG CCCACACGGG AGCAGCCTCG 
CGCTTCTCCC TGGAACACCC CACGATGGAG CCCCACCCCG AGGAGATGCC CCTTGCACTG 
GGACTGGACC TGCTGACTGT GAGGAAGTCT GGGGTCAGCC GGACACACTC CCTGCCCAAT 
GACAGCTACA TGTTCCGGGA CGGAGGCACC GCTGAGGGGT CCCTAGGATG CAGAAGCTGG 
GGGCTCCCCA AAGCTCAGTC AGGCTCTGTC TTATCTGTCC ACTCGCAGCC AGCAGACACC 
AGCTACATCC TGCAGCTTCC CAAAGACACA CCTCACCTGC TCCAGCCCCA CAGCGCTCCC 
ACCTGGGGCA CCATTCCCAA ACTGCCCCCA CCAGGCCGCT CCCCCTTGGC TCAGAGGCCA 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAT TCTCTGGACG TTCAGGGCCT GGGCAGCCGG 
GAAGACCTGC TGTCCGAGGT GAGTGGGCCC TCCCCACCTC TGGCCCGGGC CTCCTCCTTC 
TGGGGCCACT TGGGCGCCCA GATGCAGCAG CATCCCCACC GCCAGAGCAA GATCTCGAAG 
CACATGCCCC CGCCAGCCCC CCGCCCAGGC TCAGAAACCA CCTGGAGCAA AGACCCACCC 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
ACCGTGGGCA GCCAGGAGGA GCCCCCGTCC CCACGGGACC TGAAGAAGTG TCACAGCGTG 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGCG TCCTGGCTGG ATGAACAGAG GAGACACTCC 
ATCGCGGTCA GCTGCCTGGA CGGCGGCTCC CAACCCAGCC TGGGCCCCGG CCCCTCTAGC 
CTTGGCGGCC AGCCTCTTGG GGGACCTGGA AGCCGGCCCA AGAAAAAACT CAGCCCACCT 
AGTATCTCCA TAGACCCCCC AGAGAGTCAG GGCCCTCGGC CCCCACCCAG CCCCGGCGTC 
TGCCTCCGGC GGAGGGCTCC GTCCAGCGAC TCCAGGGACC CCTCGGCCTC CGGCCCCCCT 
GACAGCATGG CTGCCTCGCC CCCCCCAAAG AAAGACGTGC TGAGTCTCTC TGGTTTAGCC 
TCTGACCCAG CAGACCTGGA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972122.1
CDS	504..7370
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSSSAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116647.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116647.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAT CTAATGCTGG ACGATGTAAT TGCTTCCGGC 
AGCTCATCCA GCGCGGCGCC AGAGGCCCAG TGTAAACCCT ACTACTCAGA CTACTCCCGA 
TTCCGGCTCC TCGTCCACCA CTTGTGTACC AGCCACTATC TGGACCTCTT CATCACGGGT 
GTCATTGGTC TGAATGTGGT CACCATGGCC ATGGAGCACT ACCAGCAGCC CCAGATCCTG 
GACGAGGCCT TGAAGATCTG CAACTATATC TTCACCGTCA TCTTTGTCTT GGAGTCAGTT 
TTTAAACTCG TGGCCTTTGG CTTCCGTCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTGCTGTC CATCATGGGC ATCACGCTGG AGGAGATTGA GGTCAATGCC 
TCGCTGCCCA TCAACCCCAC CATCATCCGC ATCATGAGAG TGCTGCGCAT TGCCCGAGTG 
CTGAAGCTGC TGAAGATGGC TGTGGGCATG CGGGCGCTGC TGGACACAGT CATGCAGGCC 
CTGCCTCAGG TGGGGAACCT GGGACTTCTC TTCATGTTGT TGTTTTTCAT CTTTGCAGCT 
CTGGGTGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CGCACCCCTG CGAGGGCCTG 
GGCCGGCATG CCACCTTTCG GAACTTTGGC ATGGCCTTCC TGACCCTCTT CCGAGTGTCC 
ACAGGTGACA ACTGGAACGG CATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACATGCTACA ATACAGTCAT CTCCCCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT GATCGCTGTG CTGATGAAGC ACCTGGAGGA AAGCAACAAG 
GAGGCCAAAG AGGAGGCCGA GCTGGAGGCT GAGCTGGAGC TGGAGATGAA GACGCTCAGC 
CCACAGCCCC ACTCGCCACT GGGCAGCCCC TTCCTCTGGC CGGGGGTCGA GGGCCCCGAC 
AGCCCCGATA GCCCCAAGCT CAGGGCCCCG CACACCGCGG CCCACACGGG AGCAGCCTCG 
CGCTTCTCCC TGGAACACCC CACGATGGAG CCCCACCCCG AGGAGATGCC CCTTGCACTG 
GGACTGGACC TGCTGACTGT GAGGAAGTCT GGGGTCAGCC GGACACACTC CCTGCCCAAT 
GACAGCTACA TGTTCCGGGA CGGAGGCACC GCTGAGGGGT CCCTAGGATG CAGAAGCTGG 
GGGCTCCCCA AAGCTCAGTC AGGCTCTGTC TTATCTGTCC ACTCGCAGCC AGCAGACACC 
AGCTACATCC TGCAGCTTCC CAAAGACACA CCTCACCTGC TCCAGCCCCA CAGCGCTCCC 
ACCTGGGGCA CCATTCCCAA ACTGCCCCCA CCAGGCCGCT CCCCCTTGGC TCAGAGGCCA 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAT TCTCTGGACG TTCAGGGCCT GGGCAGCCGG 
GAAGACCTGC TGTCCGAGGT GAGTGGGCCC TCCCCACCTC TGGCCCGGGC CTCCTCCTTC 
TGGGGCCACT TGGGCGCCCA GATGCAGCAG CATCCCCACC GCCAGAGCAA GATCTCGAAG 
CACATGCCCC CGCCAGCCCC CCGCCCAGGC TCAGAAACCA CCTGGAGCAA AGACCCACCC 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
ACCGTGGGCA GCCAGGAGGA GCCCCCGTCC CCACGGGACC TGAAGAAGTG TCACAGCGTG 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGCG TCCTGGCTGG ATGAACAGAG GAGACACTCC 
ATCGCGGTCA GCTGCCTGGA CGGCGGCTCC CAACCCAGCC TGGGCCCCGG CCCCTCTAGC 
CTTGGCGGCC AGCCTCTTGG GGGACCTGGA AGCCGGCCCA AGAAAAAACT CAGCCCACCT 
AGTATCTCCA TAGACCCCCC AGAGAGTCAG GGCCCTCGGC CCCCACCCAG CCCCGGCGTC 
TGCCTCCGGC GGAGGGCTCC GTCCAGCGAC TCCAGGGACC CCTCGGCCTC CGGCCCCCCT 
GACAGCATGG CTGCCTCGCC CCCCCCAAAG AAAGACGTGC TGAGTCTCTC TGGTTTAGCC 
TCTGACCCAG CAGACCTGGA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116648.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972123.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X25, mRNA.

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CloneID	OCe38396
Clone ID Related Accession (Same CDS sequence)	XM_014116648.1 , XM_014116648.2
Accession Version	XM_014116648.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X25
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA GGCCCAGTGT 
AAACCCTACT ACTCAGACTA CTCCCGATTC CGGCTCCTCG TCCACCACTT GTGTACCAGC 
CACTATCTGG ACCTCTTCAT CACGGGTGTC ATTGGTCTGA ATGTGGTCAC CATGGCCATG 
GAGCACTACC AGCAGCCCCA GATCCTGGAC GAGGCCTTGA AGATCTGCAA CTATATCTTC 
ACCGTCATCT TTGTCTTGGA GTCAGTTTTT AAACTCGTGG CCTTTGGCTT CCGTCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCCATCGTGC TGCTGTCCAT CATGGGCATC 
ACGCTGGAGG AGATTGAGGT CAATGCCTCG CTGCCCATCA ACCCCACCAT CATCCGCATC 
ATGAGAGTGC TGCGCATTGC CCGAGTGCTG AAGCTGCTGA AGATGGCTGT GGGCATGCGG 
GCGCTGCTGG ACACAGTCAT GCAGGCCCTG CCTCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGTTGTTGT TTTTCATCTT TGCAGCTCTG GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACGC ACCCCTGCGA GGGCCTGGGC CGGCATGCCA CCTTTCGGAA CTTTGGCATG 
GCCTTCCTGA CCCTCTTCCG AGTGTCCACA GGTGACAACT GGAACGGCAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACA TGCTACAATA CAGTCATCTC CCCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG 
ATGAAGCACC TGGAGGAAAG CAACAAGGAG GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG 
CTGGAGCTGG AGATGAAGAC GCTCAGCCCA CAGCCCCACT CGCCACTGGG CAGCCCCTTC 
CTCTGGCCGG GGGTCGAGGG CCCCGACAGC CCCGATAGCC CCAAGCTCAG GGCCCCGCAC 
ACCGCGGCCC ACACGGGAGC AGCCTCGCGC TTCTCCCTGG AACACCCCAC GATGGAGCCC 
CACCCCGAGG AGATGCCCCT TGCACTGGGA CTGGACCTGC TGACTGTGAG GAAGTCTGGG 
GTCAGCCGGA CACACTCCCT GCCCAATGAC AGCTACATGT TCCGGGACGG AGGCACCGCT 
GAGGGGTCCC TAGGATGCAG AAGCTGGGGG CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA 
TCTGTCCACT CGCAGCCAGC AGACACCAGC TACATCCTGC AGCTTCCCAA AGACACACCT 
CACCTGCTCC AGCCCCACAG CGCTCCCACC TGGGGCACCA TTCCCAAACT GCCCCCACCA 
GGCCGCTCCC CCTTGGCTCA GAGGCCACTC AGGCGCCAGG CAGCAATAAG GACTGATTCT 
CTGGACGTTC AGGGCCTGGG CAGCCGGGAA GACCTGCTGT CCGAGGTGAG TGGGCCCTCC 
CCACCTCTGG CCCGGGCCTC CTCCTTCTGG GGCCACTTGG GCGCCCAGAT GCAGCAGCAT 
CCCCACCGCC AGAGCAAGAT CTCGAAGCAC ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA 
GAAACCACCT GGAGCAAAGA CCCACCCGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGACC GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA 
CGGGACCTGA AGAAGTGTCA CAGCGTGGAG GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC 
TGGCTGGATG AACAGAGGAG ACACTCCATC GCGGTCAGCT GCCTGGACGG CGGCTCCCAA 
CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC 
CGGCCCAAGA AAAAACTCAG CCCACCTAGT ATCTCCATAG ACCCCCCAGA GAGTCAGGGC 
CCTCGGCCCC CACCCAGCCC CGGCGTCTGC CTCCGGCGGA GGGCTCCGTC CAGCGACTCC 
AGGGACCCCT CGGCCTCCGG CCCCCCTGAC AGCATGGCTG CCTCGCCCCC CCCAAAGAAA 
GACGTGCTGA GTCTCTCTGG TTTAGCCTCT GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972123.1
CDS	526..7359
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116648.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116648.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA GGCCCAGTGT 
AAACCCTACT ACTCAGACTA CTCCCGATTC CGGCTCCTCG TCCACCACTT GTGTACCAGC 
CACTATCTGG ACCTCTTCAT CACGGGTGTC ATTGGTCTGA ATGTGGTCAC CATGGCCATG 
GAGCACTACC AGCAGCCCCA GATCCTGGAC GAGGCCTTGA AGATCTGCAA CTATATCTTC 
ACCGTCATCT TTGTCTTGGA GTCAGTTTTT AAACTCGTGG CCTTTGGCTT CCGTCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCCATCGTGC TGCTGTCCAT CATGGGCATC 
ACGCTGGAGG AGATTGAGGT CAATGCCTCG CTGCCCATCA ACCCCACCAT CATCCGCATC 
ATGAGAGTGC TGCGCATTGC CCGAGTGCTG AAGCTGCTGA AGATGGCTGT GGGCATGCGG 
GCGCTGCTGG ACACAGTCAT GCAGGCCCTG CCTCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGTTGTTGT TTTTCATCTT TGCAGCTCTG GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACGC ACCCCTGCGA GGGCCTGGGC CGGCATGCCA CCTTTCGGAA CTTTGGCATG 
GCCTTCCTGA CCCTCTTCCG AGTGTCCACA GGTGACAACT GGAACGGCAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACA TGCTACAATA CAGTCATCTC CCCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG 
ATGAAGCACC TGGAGGAAAG CAACAAGGAG GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG 
CTGGAGCTGG AGATGAAGAC GCTCAGCCCA CAGCCCCACT CGCCACTGGG CAGCCCCTTC 
CTCTGGCCGG GGGTCGAGGG CCCCGACAGC CCCGATAGCC CCAAGCTCAG GGCCCCGCAC 
ACCGCGGCCC ACACGGGAGC AGCCTCGCGC TTCTCCCTGG AACACCCCAC GATGGAGCCC 
CACCCCGAGG AGATGCCCCT TGCACTGGGA CTGGACCTGC TGACTGTGAG GAAGTCTGGG 
GTCAGCCGGA CACACTCCCT GCCCAATGAC AGCTACATGT TCCGGGACGG AGGCACCGCT 
GAGGGGTCCC TAGGATGCAG AAGCTGGGGG CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA 
TCTGTCCACT CGCAGCCAGC AGACACCAGC TACATCCTGC AGCTTCCCAA AGACACACCT 
CACCTGCTCC AGCCCCACAG CGCTCCCACC TGGGGCACCA TTCCCAAACT GCCCCCACCA 
GGCCGCTCCC CCTTGGCTCA GAGGCCACTC AGGCGCCAGG CAGCAATAAG GACTGATTCT 
CTGGACGTTC AGGGCCTGGG CAGCCGGGAA GACCTGCTGT CCGAGGTGAG TGGGCCCTCC 
CCACCTCTGG CCCGGGCCTC CTCCTTCTGG GGCCACTTGG GCGCCCAGAT GCAGCAGCAT 
CCCCACCGCC AGAGCAAGAT CTCGAAGCAC ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA 
GAAACCACCT GGAGCAAAGA CCCACCCGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGACC GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA 
CGGGACCTGA AGAAGTGTCA CAGCGTGGAG GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC 
TGGCTGGATG AACAGAGGAG ACACTCCATC GCGGTCAGCT GCCTGGACGG CGGCTCCCAA 
CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC 
CGGCCCAAGA AAAAACTCAG CCCACCTAGT ATCTCCATAG ACCCCCCAGA GAGTCAGGGC 
CCTCGGCCCC CACCCAGCCC CGGCGTCTGC CTCCGGCGGA GGGCTCCGTC CAGCGACTCC 
AGGGACCCCT CGGCCTCCGG CCCCCCTGAC AGCATGGCTG CCTCGCCCCC CCCAAAGAAA 
GACGTGCTGA GTCTCTCTGG TTTAGCCTCT GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116648.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972123.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X21, mRNA.

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CloneID	OCe38396
Clone ID Related Accession (Same CDS sequence)	XM_014116648.1 , XM_014116648.2
Accession Version	XM_014116648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA GGCCCAGTGT 
AAACCCTACT ACTCAGACTA CTCCCGATTC CGGCTCCTCG TCCACCACTT GTGTACCAGC 
CACTATCTGG ACCTCTTCAT CACGGGTGTC ATTGGTCTGA ATGTGGTCAC CATGGCCATG 
GAGCACTACC AGCAGCCCCA GATCCTGGAC GAGGCCTTGA AGATCTGCAA CTATATCTTC 
ACCGTCATCT TTGTCTTGGA GTCAGTTTTT AAACTCGTGG CCTTTGGCTT CCGTCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCCATCGTGC TGCTGTCCAT CATGGGCATC 
ACGCTGGAGG AGATTGAGGT CAATGCCTCG CTGCCCATCA ACCCCACCAT CATCCGCATC 
ATGAGAGTGC TGCGCATTGC CCGAGTGCTG AAGCTGCTGA AGATGGCTGT GGGCATGCGG 
GCGCTGCTGG ACACAGTCAT GCAGGCCCTG CCTCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGTTGTTGT TTTTCATCTT TGCAGCTCTG GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACGC ACCCCTGCGA GGGCCTGGGC CGGCATGCCA CCTTTCGGAA CTTTGGCATG 
GCCTTCCTGA CCCTCTTCCG AGTGTCCACA GGTGACAACT GGAACGGCAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACA TGCTACAATA CAGTCATCTC CCCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG 
ATGAAGCACC TGGAGGAAAG CAACAAGGAG GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG 
CTGGAGCTGG AGATGAAGAC GCTCAGCCCA CAGCCCCACT CGCCACTGGG CAGCCCCTTC 
CTCTGGCCGG GGGTCGAGGG CCCCGACAGC CCCGATAGCC CCAAGCTCAG GGCCCCGCAC 
ACCGCGGCCC ACACGGGAGC AGCCTCGCGC TTCTCCCTGG AACACCCCAC GATGGAGCCC 
CACCCCGAGG AGATGCCCCT TGCACTGGGA CTGGACCTGC TGACTGTGAG GAAGTCTGGG 
GTCAGCCGGA CACACTCCCT GCCCAATGAC AGCTACATGT TCCGGGACGG AGGCACCGCT 
GAGGGGTCCC TAGGATGCAG AAGCTGGGGG CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA 
TCTGTCCACT CGCAGCCAGC AGACACCAGC TACATCCTGC AGCTTCCCAA AGACACACCT 
CACCTGCTCC AGCCCCACAG CGCTCCCACC TGGGGCACCA TTCCCAAACT GCCCCCACCA 
GGCCGCTCCC CCTTGGCTCA GAGGCCACTC AGGCGCCAGG CAGCAATAAG GACTGATTCT 
CTGGACGTTC AGGGCCTGGG CAGCCGGGAA GACCTGCTGT CCGAGGTGAG TGGGCCCTCC 
CCACCTCTGG CCCGGGCCTC CTCCTTCTGG GGCCACTTGG GCGCCCAGAT GCAGCAGCAT 
CCCCACCGCC AGAGCAAGAT CTCGAAGCAC ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA 
GAAACCACCT GGAGCAAAGA CCCACCCGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGACC GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA 
CGGGACCTGA AGAAGTGTCA CAGCGTGGAG GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC 
TGGCTGGATG AACAGAGGAG ACACTCCATC GCGGTCAGCT GCCTGGACGG CGGCTCCCAA 
CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC 
CGGCCCAAGA AAAAACTCAG CCCACCTAGT ATCTCCATAG ACCCCCCAGA GAGTCAGGGC 
CCTCGGCCCC CACCCAGCCC CGGCGTCTGC CTCCGGCGGA GGGCTCCGTC CAGCGACTCC 
AGGGACCCCT CGGCCTCCGG CCCCCCTGAC AGCATGGCTG CCTCGCCCCC CCCAAAGAAA 
GACGTGCTGA GTCTCTCTGG TTTAGCCTCT GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972123.1
CDS	504..7337
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116648.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116648.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCTGA GGCCCAGTGT 
AAACCCTACT ACTCAGACTA CTCCCGATTC CGGCTCCTCG TCCACCACTT GTGTACCAGC 
CACTATCTGG ACCTCTTCAT CACGGGTGTC ATTGGTCTGA ATGTGGTCAC CATGGCCATG 
GAGCACTACC AGCAGCCCCA GATCCTGGAC GAGGCCTTGA AGATCTGCAA CTATATCTTC 
ACCGTCATCT TTGTCTTGGA GTCAGTTTTT AAACTCGTGG CCTTTGGCTT CCGTCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCCATCGTGC TGCTGTCCAT CATGGGCATC 
ACGCTGGAGG AGATTGAGGT CAATGCCTCG CTGCCCATCA ACCCCACCAT CATCCGCATC 
ATGAGAGTGC TGCGCATTGC CCGAGTGCTG AAGCTGCTGA AGATGGCTGT GGGCATGCGG 
GCGCTGCTGG ACACAGTCAT GCAGGCCCTG CCTCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGTTGTTGT TTTTCATCTT TGCAGCTCTG GGTGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACGC ACCCCTGCGA GGGCCTGGGC CGGCATGCCA CCTTTCGGAA CTTTGGCATG 
GCCTTCCTGA CCCTCTTCCG AGTGTCCACA GGTGACAACT GGAACGGCAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACA TGCTACAATA CAGTCATCTC CCCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTGAT CGCTGTGCTG 
ATGAAGCACC TGGAGGAAAG CAACAAGGAG GCCAAAGAGG AGGCCGAGCT GGAGGCTGAG 
CTGGAGCTGG AGATGAAGAC GCTCAGCCCA CAGCCCCACT CGCCACTGGG CAGCCCCTTC 
CTCTGGCCGG GGGTCGAGGG CCCCGACAGC CCCGATAGCC CCAAGCTCAG GGCCCCGCAC 
ACCGCGGCCC ACACGGGAGC AGCCTCGCGC TTCTCCCTGG AACACCCCAC GATGGAGCCC 
CACCCCGAGG AGATGCCCCT TGCACTGGGA CTGGACCTGC TGACTGTGAG GAAGTCTGGG 
GTCAGCCGGA CACACTCCCT GCCCAATGAC AGCTACATGT TCCGGGACGG AGGCACCGCT 
GAGGGGTCCC TAGGATGCAG AAGCTGGGGG CTCCCCAAAG CTCAGTCAGG CTCTGTCTTA 
TCTGTCCACT CGCAGCCAGC AGACACCAGC TACATCCTGC AGCTTCCCAA AGACACACCT 
CACCTGCTCC AGCCCCACAG CGCTCCCACC TGGGGCACCA TTCCCAAACT GCCCCCACCA 
GGCCGCTCCC CCTTGGCTCA GAGGCCACTC AGGCGCCAGG CAGCAATAAG GACTGATTCT 
CTGGACGTTC AGGGCCTGGG CAGCCGGGAA GACCTGCTGT CCGAGGTGAG TGGGCCCTCC 
CCACCTCTGG CCCGGGCCTC CTCCTTCTGG GGCCACTTGG GCGCCCAGAT GCAGCAGCAT 
CCCCACCGCC AGAGCAAGAT CTCGAAGCAC ATGCCCCCGC CAGCCCCCCG CCCAGGCTCA 
GAAACCACCT GGAGCAAAGA CCCACCCGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGACC GTGGGCAGCC AGGAGGAGCC CCCGTCCCCA 
CGGGACCTGA AGAAGTGTCA CAGCGTGGAG GCCCAGAGCT GCCGGCGCAG GCCTGCGTCC 
TGGCTGGATG AACAGAGGAG ACACTCCATC GCGGTCAGCT GCCTGGACGG CGGCTCCCAA 
CCCAGCCTGG GCCCCGGCCC CTCTAGCCTT GGCGGCCAGC CTCTTGGGGG ACCTGGAAGC 
CGGCCCAAGA AAAAACTCAG CCCACCTAGT ATCTCCATAG ACCCCCCAGA GAGTCAGGGC 
CCTCGGCCCC CACCCAGCCC CGGCGTCTGC CTCCGGCGGA GGGCTCCGTC CAGCGACTCC 
AGGGACCCCT CGGCCTCCGG CCCCCCTGAC AGCATGGCTG CCTCGCCCCC CCCAAAGAAA 
GACGTGCTGA GTCTCTCTGG TTTAGCCTCT GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116649.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972124.1 Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X28, mRNA.

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CloneID	OCe38397
Clone ID Related Accession (Same CDS sequence)	XM_014116649.1 , XM_014116649.2
Accession Version	XM_014116649.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X28
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG ATGGAGCCCC ACCCCGAGGA GATGCCCCTT 
GCACTGGGAC TGGACCTGCT GACTGTGAGG AAGTCTGGGG TCAGCCGGAC ACACTCCCTG 
CCCAATGACA GCTACATGTT CCGGGACGGA GGCACCGCTG AGGGGTCCCT AGGATGCAGA 
AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC TCTGTCTTAT CTGTCCACTC GCAGCCAGCA 
GACACCAGCT ACATCCTGCA GCTTCCCAAA GACACACCTC ACCTGCTCCA GCCCCACAGC 
GCTCCCACCT GGGGCACCAT TCCCAAACTG CCCCCACCAG GCCGCTCCCC CTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATAAGG ACTGATTCTC TGGACGTTCA GGGCCTGGGC 
AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT GGGCCCTCCC CACCTCTGGC CCGGGCCTCC 
TCCTTCTGGG GCCACTTGGG CGCCCAGATG CAGCAGCATC CCCACCGCCA GAGCAAGATC 
TCGAAGCACA TGCCCCCGCC AGCCCCCCGC CCAGGCTCAG AAACCACCTG GAGCAAAGAC 
CCACCCGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGACCG TGGGCAGCCA GGAGGAGCCC CCGTCCCCAC GGGACCTGAA GAAGTGTCAC 
AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG CCTGCGTCCT GGCTGGATGA ACAGAGGAGA 
CACTCCATCG CGGTCAGCTG CCTGGACGGC GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC 
TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA CCTGGAAGCC GGCCCAAGAA AAAACTCAGC 
CCACCTAGTA TCTCCATAGA CCCCCCAGAG AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC 
GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC AGCGACTCCA GGGACCCCTC GGCCTCCGGC 
CCCCCTGACA GCATGGCTGC CTCGCCCCCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT 
TTAGCCTCTG ACCCAGCAGA CCTGGACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972124.1
CDS	526..7338
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116649.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116649.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG ATGGAGCCCC ACCCCGAGGA GATGCCCCTT 
GCACTGGGAC TGGACCTGCT GACTGTGAGG AAGTCTGGGG TCAGCCGGAC ACACTCCCTG 
CCCAATGACA GCTACATGTT CCGGGACGGA GGCACCGCTG AGGGGTCCCT AGGATGCAGA 
AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC TCTGTCTTAT CTGTCCACTC GCAGCCAGCA 
GACACCAGCT ACATCCTGCA GCTTCCCAAA GACACACCTC ACCTGCTCCA GCCCCACAGC 
GCTCCCACCT GGGGCACCAT TCCCAAACTG CCCCCACCAG GCCGCTCCCC CTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATAAGG ACTGATTCTC TGGACGTTCA GGGCCTGGGC 
AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT GGGCCCTCCC CACCTCTGGC CCGGGCCTCC 
TCCTTCTGGG GCCACTTGGG CGCCCAGATG CAGCAGCATC CCCACCGCCA GAGCAAGATC 
TCGAAGCACA TGCCCCCGCC AGCCCCCCGC CCAGGCTCAG AAACCACCTG GAGCAAAGAC 
CCACCCGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGACCG TGGGCAGCCA GGAGGAGCCC CCGTCCCCAC GGGACCTGAA GAAGTGTCAC 
AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG CCTGCGTCCT GGCTGGATGA ACAGAGGAGA 
CACTCCATCG CGGTCAGCTG CCTGGACGGC GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC 
TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA CCTGGAAGCC GGCCCAAGAA AAAACTCAGC 
CCACCTAGTA TCTCCATAGA CCCCCCAGAG AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC 
GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC AGCGACTCCA GGGACCCCTC GGCCTCCGGC 
CCCCCTGACA GCATGGCTGC CTCGCCCCCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT 
TTAGCCTCTG ACCCAGCAGA CCTGGACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_014116649.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972124.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X24, mRNA.

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CloneID	OCe38397
Clone ID Related Accession (Same CDS sequence)	XM_014116649.1 , XM_014116649.2
Accession Version	XM_014116649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG ATGGAGCCCC ACCCCGAGGA GATGCCCCTT 
GCACTGGGAC TGGACCTGCT GACTGTGAGG AAGTCTGGGG TCAGCCGGAC ACACTCCCTG 
CCCAATGACA GCTACATGTT CCGGGACGGA GGCACCGCTG AGGGGTCCCT AGGATGCAGA 
AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC TCTGTCTTAT CTGTCCACTC GCAGCCAGCA 
GACACCAGCT ACATCCTGCA GCTTCCCAAA GACACACCTC ACCTGCTCCA GCCCCACAGC 
GCTCCCACCT GGGGCACCAT TCCCAAACTG CCCCCACCAG GCCGCTCCCC CTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATAAGG ACTGATTCTC TGGACGTTCA GGGCCTGGGC 
AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT GGGCCCTCCC CACCTCTGGC CCGGGCCTCC 
TCCTTCTGGG GCCACTTGGG CGCCCAGATG CAGCAGCATC CCCACCGCCA GAGCAAGATC 
TCGAAGCACA TGCCCCCGCC AGCCCCCCGC CCAGGCTCAG AAACCACCTG GAGCAAAGAC 
CCACCCGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGACCG TGGGCAGCCA GGAGGAGCCC CCGTCCCCAC GGGACCTGAA GAAGTGTCAC 
AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG CCTGCGTCCT GGCTGGATGA ACAGAGGAGA 
CACTCCATCG CGGTCAGCTG CCTGGACGGC GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC 
TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA CCTGGAAGCC GGCCCAAGAA AAAACTCAGC 
CCACCTAGTA TCTCCATAGA CCCCCCAGAG AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC 
GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC AGCGACTCCA GGGACCCCTC GGCCTCCGGC 
CCCCCTGACA GCATGGCTGC CTCGCCCCCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT 
TTAGCCTCTG ACCCAGCAGA CCTGGACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972124.1
CDS	504..7316
Translation	MDEEEDGAGTEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPVPRGGQEPQSGGSCSRSHRRPSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDKDASGSRRLMLPPPSTPALSGGPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKEKALVEVAPTSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGAGGPDSCPYCARAGAGQVELAGHEMPDSDSEAVYELTQDVHHGDLRDPHSRHRQSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLEGNGDRKKRLASVVSLGEHPELRRNLLPPLIIHTAATPMSLPKSSSTGLAEVLGPASRRTSSSGSAEPGAAHEMKSPPSARSTPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERASPAGSDRRHGGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARALRPDDPPLDGDDGDDEGNLSRGERIRVWIRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCTEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKLRAPHTAAHTGAASRFSLEHPTMEPHPEEMPLALGLDLLTVRKSGVSRTHSLPNDSYMFRDGGTAEGSLGCRSWGLPKAQSGSVLSVHSQPADTSYILQLPKDTPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSPPLARASSFWGHLGAQMQQHPHRQSKISKHMPPPAPRPGSETTWSKDPPETRSSLELDTELSWISGDLLTVGSQEEPPSPRDLKKCHSVEAQSCRRRPASWLDEQRRHSIAVSCLDGGSQPSLGPGPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPPSPGVCLRRRAPSSDSRDPSASGPPDSMAASPPPKKDVLSLSGLASDPADLDP

Target ORF information:

RefSeq Version	XM_014116649.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116649.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCACC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ATCTGTCGGG GGCCGGGGGC CGGCCGGGGC CGGGGTCGGC GGAGAAGGAC 
CCGGGCAGCG CCGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG CATGTTGGTC ATCCTTCTCA ATTGTGTGAC CCTGGGCATG 
TTCCGGCCCT GTGAGGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATCCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTCGGGA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAACG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTGCGCCC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTT 
GTCACACTGC TGCTGGACAC ACTACCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT TGTTGGCGTC CAGCTGTGGG CAGGGCTGCT TCGTAACCGA 
TGCTTCCTGC CGGAGAATTT CAGCCTCCCC CTGAGTGTGG ACCTGGAGCG CTATTACCAG 
ACCGAGAACG AGGACGAGAA CCCCTTCATC TGCTCCCAGC CACGGGAGAA CGGCATGCGC 
TCATGCAGGA GTGTGCCCAC GCTGCGTGGG GAGGGCGGTG GCGGCCCCCC CTGCGGCCTG 
GACTATGAGG CCTACAACAG CTCCAGCAAC ACCACCTGCG TCAACTGGAA CCAGTATTAC 
ACCAACTGCT CGGCAGGGGA GCACAACCCC TTCAAGGGCG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATTGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTCGTGA TGGATGCTCA TTCCTTCTAC AATTTCATCT ACTTCATCCT CCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAAAGCCA GCTGATGCGA GAGCAACGTG TGCGGTTCCT GTCCAACGCC 
AGCACCCTGG CCAGCTTCTC TGAGCCGGGC AGCTGCTACG AGGAGCTGCT CAAGTACCTG 
GTGTACATCC TCCGTAAAGC GGCCCGCAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTCGGCC TGCTGAGCAG CCCAGTGCCC CGTGGAGGCC AGGAGCCCCA GTCTGGCGGC 
AGCTGCTCTC GCTCCCACCG CCGTCCATCC GTCCACCACC TGGTGCACCA CCACCACCAC 
CATCACCACC ACTACCATCT GGGCAATGGG ACGCTGAGGG CCCCCCGGGC CAGCCCGGAG 
ATTCAGGACA AGGATGCCAG TGGGTCCCGC CGGCTCATGC TGCCACCACC CTCGACGCCA 
GCCCTCTCTG GGGGCCCTCC AGGGGGTGCA GAATCTGTGC ACAGCTTCTA CCATGCCGAC 
TGCCACCTGG AGCCGGTCCG CTGCCAGGCA CCCCCTCCCA GGTCACCATC TGAGGCGTCG 
GGCAGGACTG TGGGCAGCGG GAAGGTGTAC CCCACTGTGC ACACCAGCCC TCCACCAGAG 
ATGCTGAAGG AGAAAGCGCT AGTGGAGGTG GCCCCCACTT CTGGACCCCC CACCCTCACC 
AGCCTCAACA TCCCGCCCGG GCCCTACAGC TCCATGCACA AGCTACTAGA GACACAGAGC 
ACAGGCGCCT GCCAAAGCTC TTGCAAGATC TCCAGCCCTT GCCTGAAGGC AGACAGTGGA 
GCCGGTGGCC CAGACAGCTG CCCCTACTGT GCCCGGGCGG GGGCAGGGCA GGTGGAGCTC 
GCCGGCCACG AGATGCCTGA CTCAGACAGC GAGGCAGTTT ATGAGCTCAC ACAAGACGTC 
CACCACGGTG ATCTCCGGGA CCCCCACAGC CGGCACCGCC AGAGCCTGGG GCTGGACGTG 
GAGCCCAACT CTGTGCTGGC CTTCTGGAGG CTGATCTGTG ACACTTTCCG GAAGATTGTG 
GACAGCAAGT ACTTTGGTCG GGGAATCATG ATTGCTATCC TGGTCAACAC GCTCAGCATG 
GGCATCGAGT ACCATGAACA GCCCGAGGAG CTGACCAACG CCCTAGAAAT TAGCAACATC 
GTCTTCACCA GCCTCTTTGC CCTGGAGATG CTGCTGAAGC TGCTGGTGTA CGGTCCCTTT 
GGCTACATCA AGAACCCCTA CAATATCTTC GATGGCGTCA TTGTTGTCAT CAGCGTGTGG 
GAGATCGTGG GCCAGCAGGG GGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TGGTGCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTACTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCATCTCTT TGGCTGCAAA TTTGCATCTG AGCGGGACGG GGACACGCTG 
CCGGACCGGA AGAATTTTGA CTCCTTGCTC TGGGCCATCG TCACCGTCTT TCAGATCCTG 
ACCCAGGAGG ACTGGAACAA GGTGCTCTAC AATGGAATGG CCTCCACATC ATCCTGGGCC 
GCCCTTTATT TCATTGCCCT CATGACCTTT GGCAACTACG TGCTCTTTAA TCTGCTTGTC 
GCCATTCTGG TGGAGGGCTT CCAGGCGGAG GAAATCAGCA AACGGGAAGA TGCGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCGAC TCCCAGGGGG GAGATGCCAC CAAGTCCGAA 
TCAGAGCCAG ATTTCTTCTC ACCCAGCCTG GAGGGCAATG GGGACAGGAA GAAGCGCTTG 
GCCTCAGTGG TGTCCCTGGG AGAGCACCCA GAGCTGCGGA GGAACCTGCT GCCACCTCTT 
ATCATCCACA CGGCGGCCAC ACCCATGTCG CTCCCCAAGA GTTCCAGCAC TGGCCTGGCC 
GAGGTGCTGG GCCCTGCCTC CCGCCGCACC AGCAGCAGCG GGTCTGCTGA GCCAGGGGCG 
GCCCATGAGA TGAAGTCACC GCCGAGCGCC CGCAGCACTC CGCACAGCCC CTGGAGTGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGAGC CCCCAGCCTG 
AAGCGGAGGA GCCCGAGTGG GGAGCGGCGG TCCCTGTTGT CCGGCGAGGG TCGGGAGAGC 
CAGGATGAGG AGGAGAGCTC TGAAGAGGAG CGGGCCAGCC CAGCGGGCAG CGACCGGCGC 
CATGGAGGCT CCCTGGAGCG GGAGGCCAAG AGCTCCTTCG ACCTGCCGGA CACGCTGCAA 
GTGCCCGGAC TGCACCGCAC GGCCAGTGGC CGCAGCTCAG CCTCTGAGCA CCAGGACTGC 
AACGGCAAGT CGGCCTCGGG GCGCCTGGCC CGGGCCCTGC GGCCCGACGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA CGAGGGCAAC CTGAGCAGAG GGGAGCGGAT AAGAGTGTGG 
ATCCGAGCCC GGCTGCCTGC CTGCTGCCGC GAGCGTGATT CCTGGTCCGC GTACATCTTC 
CCTCCTCAGT CCAGGTTCCG CCTCTTATGT CACCGAATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TTGTCATCAT CTTCCTCAAC TGCATCACCA TCGCCATGGA GCGTCCCAAG 
ATCGACCCCC ACAGTGCTGA GCGCATCTTC CTGACCCTCT CCAATTACAT CTTTACCGCA 
GTCTTTCTGG CAGAGATGAC CGTGAAGGTG GTAGCGCTGG GCTGGTGCTT CGGGGAGCAG 
GCGTACCTGC GTAGCAGCTG GAACGTTCTC GACGGGCTGC TGGTGCTCAT CTCCATCATC 
GACATCCTGG TGTCCATGGT CTCCGACAGC GGCACCAAGA TCCTGGGCAT GTTGAGGGTG 
CTGCGGCTGC TACGGACCCT GCGCCCACTC AGGGTTATTA GCAGAGCCCA GGGGCTGAAG 
CTGGTAGTGG AAACGCTGAT GTCCTCACTG AAGCCCATCG GCAACATCGT GGTCATTTGT 
TGTGCCTTCT TCATCATATT TGGCATCCTG GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGCCAGG GCGAGGACAC CAGGAACATC ACCAACAAGT CTGACTGCAC CGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAATTTT GACAACCTCG GCCAGGCCCT GATGTCCCTG 
TTCGTACTGG CCTCCAAGGA CGGCTGGGTG GACATCATGT ACGACGGGCT GGACGCCGTG 
GGTGTGGACC AACAGCCCAT CATGAACCAC AACCCCTGGA TGCTGCTGTA CTTCATCTCG 
TTCCTGCTCA TTGTGGCCTT CTTTGTCCTG AACATGTTTG TGGGTGTGGT GGTGGAGAAC 
TTTCACAAGT GTCGGCAGCA CCAGGAGGAG GAGGAGGCCC GGCGGCGAGA GGAGAAACGT 
CTGCGGAGAC TGGAGAAAAA GAGAAGGAAG GCCCAGTGTA AACCCTACTA CTCAGACTAC 
TCCCGATTCC GGCTCCTCGT CCACCACTTG TGTACCAGCC ACTATCTGGA CCTCTTCATC 
ACGGGTGTCA TTGGTCTGAA TGTGGTCACC ATGGCCATGG AGCACTACCA GCAGCCCCAG 
ATCCTGGACG AGGCCTTGAA GATCTGCAAC TATATCTTCA CCGTCATCTT TGTCTTGGAG 
TCAGTTTTTA AACTCGTGGC CTTTGGCTTC CGTCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CCATCGTGCT GCTGTCCATC ATGGGCATCA CGCTGGAGGA GATTGAGGTC 
AATGCCTCGC TGCCCATCAA CCCCACCATC ATCCGCATCA TGAGAGTGCT GCGCATTGCC 
CGAGTGCTGA AGCTGCTGAA GATGGCTGTG GGCATGCGGG CGCTGCTGGA CACAGTCATG 
CAGGCCCTGC CTCAGGTGGG GAACCTGGGA CTTCTCTTCA TGTTGTTGTT TTTCATCTTT 
GCAGCTCTGG GTGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACGCA CCCCTGCGAG 
GGCCTGGGCC GGCATGCCAC CTTTCGGAAC TTTGGCATGG CCTTCCTGAC CCTCTTCCGA 
GTGTCCACAG GTGACAACTG GAACGGCATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACAT GCTACAATAC AGTCATCTCC CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTGATC GCTGTGCTGA TGAAGCACCT GGAGGAAAGC 
AACAAGGAGG CCAAAGAGGA GGCCGAGCTG GAGGCTGAGC TGGAGCTGGA GATGAAGACG 
CTCAGCCCAC AGCCCCACTC GCCACTGGGC AGCCCCTTCC TCTGGCCGGG GGTCGAGGGC 
CCCGACAGCC CCGATAGCCC CAAGCTCAGG GCCCCGCACA CCGCGGCCCA CACGGGAGCA 
GCCTCGCGCT TCTCCCTGGA ACACCCCACG ATGGAGCCCC ACCCCGAGGA GATGCCCCTT 
GCACTGGGAC TGGACCTGCT GACTGTGAGG AAGTCTGGGG TCAGCCGGAC ACACTCCCTG 
CCCAATGACA GCTACATGTT CCGGGACGGA GGCACCGCTG AGGGGTCCCT AGGATGCAGA 
AGCTGGGGGC TCCCCAAAGC TCAGTCAGGC TCTGTCTTAT CTGTCCACTC GCAGCCAGCA 
GACACCAGCT ACATCCTGCA GCTTCCCAAA GACACACCTC ACCTGCTCCA GCCCCACAGC 
GCTCCCACCT GGGGCACCAT TCCCAAACTG CCCCCACCAG GCCGCTCCCC CTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATAAGG ACTGATTCTC TGGACGTTCA GGGCCTGGGC 
AGCCGGGAAG ACCTGCTGTC CGAGGTGAGT GGGCCCTCCC CACCTCTGGC CCGGGCCTCC 
TCCTTCTGGG GCCACTTGGG CGCCCAGATG CAGCAGCATC CCCACCGCCA GAGCAAGATC 
TCGAAGCACA TGCCCCCGCC AGCCCCCCGC CCAGGCTCAG AAACCACCTG GAGCAAAGAC 
CCACCCGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGACCG TGGGCAGCCA GGAGGAGCCC CCGTCCCCAC GGGACCTGAA GAAGTGTCAC 
AGCGTGGAGG CCCAGAGCTG CCGGCGCAGG CCTGCGTCCT GGCTGGATGA ACAGAGGAGA 
CACTCCATCG CGGTCAGCTG CCTGGACGGC GGCTCCCAAC CCAGCCTGGG CCCCGGCCCC 
TCTAGCCTTG GCGGCCAGCC TCTTGGGGGA CCTGGAAGCC GGCCCAAGAA AAAACTCAGC 
CCACCTAGTA TCTCCATAGA CCCCCCAGAG AGTCAGGGCC CTCGGCCCCC ACCCAGCCCC 
GGCGTCTGCC TCCGGCGGAG GGCTCCGTCC AGCGACTCCA GGGACCCCTC GGCCTCCGGC 
CCCCCTGACA GCATGGCTGC CTCGCCCCCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT 
TTAGCCTCTG ACCCAGCAGA CCTGGACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

CACNA1G cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

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