SI cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI
Entrez Gene ID	488141
Full Name	sucrase-isomaltase
General protein information	Preferred Names sucrase-isomaltase Names sucrase-isomaltase, intestinal
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005639880.3	XP_005639937.1	sucrase-isomaltase, intestinal isoform X2
XM_545265.5	XP_545265.3	sucrase-isomaltase, intestinal isoform X1
XM_545265.6	XP_545265.3	sucrase-isomaltase, intestinal isoform X1
XM_005639880.2	XP_005639937.1	sucrase-isomaltase, intestinal isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SI	XP_545265.3
Mus musculus (house mouse)	Sis	NP_001074606.1
Homo sapiens (human)	SI	NP_001032.2
Macaca mulatta (Rhesus monkey)	SI	XP_002802979.1
Bos taurus (cattle)	SI	NP_001107661.1
Pan troglodytes (chimpanzee)	SI	XP_001159286.2
Rattus norvegicus (Norway rat)	Si	NP_037193.1

Gene Ontology

The following SI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe30577	XM_545265.6 Latest version!	Canis lupus familiaris sucrase-isomaltase (SI), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe30578	XM_005639880.3 Latest version!	Canis lupus familiaris sucrase-isomaltase (SI), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCe30577	XM_545265.5	Canis lupus familiaris sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe30578	XM_005639880.2	Canis lupus familiaris sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI ( XM_545265.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_545265.3 Canis lupus familiaris sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X1, mRNA.

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CloneID	OCe30577
Clone ID Related Accession (Same CDS sequence)	XM_545265.5 , XM_545265.6
Accession Version	XM_545265.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	sucrase-isomaltase, intestinal isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_545265.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGA AGAAATTTAC TGGATTAGAA ATCTCTCTGA TTGTCCTTTT TGTTATAGTT 
ACTATAATAG CTATTGCCCT AATTGCTGTT TTAGCAACTA AGACACCTGC TGTTGAAGCA 
ATTAGTAATT CTACTTCAAC TCCACCTACC ACTCGTACAA CCACTGTGTA TCCTGGTTCA 
GGAAAATGTC CAAGCGAGCT AAATGATCCT ATCAATGAGA GAATAAACTG CATTCCAGAA 
CAATTTCCAA CACAGGCAGT TTGTGCAATG CGAGGTTGCT GCTGGAAGCC ATGGAATAAC 
TCTATTATTC CTTGGTGCTT CTTCGTAGAT AACCATGGTT ATAATGTTGG ACAACTGACA 
GCAACAAGTA CTGGACTTGA AGCCACATTA AACAGGATAT CTTCACCTAC ATTGTTTGGA 
AATGACATTA CCCGTGTTCT CTTCACAACT CAAAACCAAA CAGCCAATCG TTTCCGGTTT 
AAGATTACTG ATCCAAATAA CAAAAGATAT GAAGTCCCTC ACCAGTTTGT AAAAGAATTT 
ACTGGAACTG CAGCTTCTAA TACATTGTAT GATGTACAGG TTATAAATAA TCCATTCAGC 
ATCAAAGTCA TTAGAAAAAG CAATGGGAGA ATTTTATTTG ATACCAGCAT TGGTCCCCTG 
GTTTACTCTG ACCAGTATTT ACAGATCTCA ACCAAACTTC CTAGTGAATA TATGTATGGT 
ATTGGGGAAC ATATTCATAA GAGATTTCGT CATGATTTAA ACTGGAAAAC ATGGCCAATT 
TTTACCCGGG ATCAACTTCC TGGTGATAAT AATAATAATT TATATGGCCA TCATACATTC 
TTCATGTGTA TTGAAGACGA GACTGGAAAA TCATTTGGTG TTTTCTTAAT GAACAGCAAT 
GCAATGGAAA TTTTCATCCA GCCTACTCCA GTAGTAACTT ACAGAGTTAC TGGTGGAATT 
CTGGACTTTT ACATCTTTCT AGGAGATACA CCAGAACAGG TAGTTCAACA GTATCAAGAG 
CTCATTGGAC GACCAGCAAT GCCAGCCTAT TGGAGTCTTG GATTCCAACT GAGTCGCTGG 
AATTATAAGT CACTTGATGT AGTGAAAGAA GTGGTAAAAA GAAATAGAGA TGCTGGCATA 
CCATTTGATA CACAAGTCAC TGATGTTGAC TATATGGAAG CCAAGAAAGA TTTTACTTAT 
GACAAAGTTG CATTTCAGGG GCTCCCTGAA TTTGTTCAAG ATTTACATGA CCATGGACAG 
AAATATGTCA TCATCTTGGA CCCTGCAATT TCCATAGATA AGCTTGCCAA TGGAGCAGCA 
TATGAAACCT ATGACAGGGG AAATGCAAAA AATGTGTGGG TAAATGATTC AGATGGGACT 
ACAGCAATTA TTGGAGAGGT ATGGCCAGGA TTAACAGTAT TCCCTGACTT TACTAATCCA 
AACTGCATAG ATTGGTGGGC AAATGAATGC AGCATTTTCT ATCAAGAAGT GAAATATGAT 
GGACTTTGGA TTGACATGAA TGAAGTTTCC AGCTTTATTC AAGGTTCACA GAAAGGATGC 
AATAACAACA AACTGAATTA TCCACCTTTT ACTCCTGATA TTCTTGACAA ACTCCTGTAT 
TCCAAAACTA TTTGCATGGA TGCTGTGCAG TACTGGGGGA AGCAGTATGA TGTTCACAGC 
CTCTATGGGT ACAGCATGGC TATAGCCACA GAGAAAGCTG TAGAAAAAGT TTTTCCTAGT 
AAGAGAAGCT TTATTCTCAC CCGGTCAACT TTTGCTGGAT CTGGGCACTA TGCTGCACAC 
TGGTTAGGAG ACAATACTGC TTCATGGGAA CAAATGGAAT GGTCTATTGC AGGAATGCTG 
GAGTTCAGTT TGTTTGGAAT GCCTTTGGTT GGAGCAGACA TCTGTGGATT TGTGGTTAAC 
ACCACAGAAG AGCTTTGCAG AAGGTGGATG CAACTTGGGG CATTTTATCC ATTTTCCAGG 
AACCATAATG CTGATGGATA TGAGCATCAG GATCCAGCAT TTTTTGGCCA GAATTCCCTT 
TTGGTTAATT CATCAAGGCA CTATTTGAAT ATTCGCTATA CCTTATTACC TTTCCTCTAT 
ACTCTGTTTT ACAAAGCCCA TGTGTTTGGA GAAACGGTAG CAAGGCCCGT TCTTCATGAG 
TTTTATGATG ACAGAGAAAG CTGGATTGAG GACACTCAGT TCTTATGGGG CCCTGCGTTA 
CTTATTACTC CCGTCTTAAA AGAGGGAACA GATACAGTGA GTGCATACAT CCCTAATGCT 
ACTTGGTATG ATTATGAAAC TGGTGCAAAA AGGCCATGGA AAAAACAGCG TGTTAATATG 
TATCTTCCGG GCGACAAAAT AGGATTACAT CTTAGAGGAG GTTATATTAT CCCCATTCAG 
CAACCTGCTG TAACTACAAC TGCAAGCCGA AAGAACCCTC TAGGACTTAT AGTTGCATTG 
GATGACAATA ACATAGCAAA AGGGGACTTT TTCTGGGATG ATGGAGAAAC TAAGAATACC 
GTACAAAATG GCAATTACAT TTTATATACT TTTTCAGTTT CCAATAACAA ATTAGATATT 
ATATGCACAC ATTCATCATA CCAGGAAGGA ACCACTTTAG CATTTGAGAC TATAAAAATC 
CTTGGGTTGA TAGATTCTGT TACACAAGTT ACAGTGGTGG AAAATAATCA GCCAATGAAG 
AATCACTACA ATTTTACTTA TACTGCTTCC AACCAGAGTC TCCTAATTTA CAATCTCAAG 
TTGAACCTTG GAGGAAACTT TACTGTTCAG TGGAATCAAA ATTTCTCAGA AAATGAAAAG 
TTTAATTGTT ACCCAGATGC AGACACTGCA ACTAAAGAAA AATGTGAAGC ACGTGGCTGT 
TTATGGGAAA CGTCTTTTAG CTCCAAAGCA CCAGAGTGTT ACTTTCCTAG ACAATCCAAC 
CCATATCTAG TCCGTTCAAC TCAGTATTCA TCAATGGGTA TAACAACTGA CCTGCAACTT 
AATCCTACAA GTGCTCGAAT AAAGCTACCT TCTGAACCCA TCTCAACTCT TCGTGTGGAA 
GTGAAATATC ACAAAAATGA TATGCTGCAG TTTAAGATTT ATGATCCCCA AACAAAGAGA 
TATGAAGTTC CAATACCTTT AAACATTCCA GACGTGCCAA CAAGTACCTA TGAAAATAGG 
CTTTATGATG TTGAAATCAG AGAAAATCCT TTTGGCATCC AGGTTCGAAG AAGAAGTACA 
GGAAGAGTTA TTTGGGATTC TCAGCTGCCA GGATTTGCTT TTAATGACCA GTTTATTCAA 
ATATCTACTC GCCTGCCATC AGAATATATA TATGGTTTTG GGGAAGTGGA ACATACAGCA 
TTTAAGAGAG ACCTGAACTG GAATACCTGG GGAATGTTCA CAAGAGACCA ACCCCCTGGT 
TATAAACTTA ATTCCTATGG ATTTCATCCC TATTACATGG CTCTGGAAGA CGAAGGCTAT 
GCTCATGGAG TTTTATTACT TAACAGCAAT GCAATGGATG TTACATTTCA GCCAACTCCT 
GCTCTAACTT ACCGTGTGAT TGGAGGAATC TTGGACTTTT ATATGTTTTT GGGCCCAACT 
CCAGAAGTTG CAACAAAGCA ATACCATGAA GTAATTGGCC GACCAGTCAT GCCACCTTAC 
TGGGCTTTGG GATTCCAAAT ATGTCGTTAT GGGTACAGAA ATACTTCACA AGTTCAGCAA 
GTATATGATG AGATGGTGGC TGCTCAGATC CCCTATGATG TTCAGTATAC AGACATTGAT 
TACATGGAAA GGCAACTAGA CTTTACAATT GATGAAAACT TCCGTGACCT TCCTGCGTTT 
GTTGACAAAA TAAGACAGGA AGGAATGAGA TACATTATTA TCCTGGATCC AGCAATTTCA 
GGAAATGAAA CAAAGTATTA CTCCGCATTT GAAAGAGGAC AGGAGAAAGA TGTTTTTGTC 
AAATGGCCTA ACACCAATGA TATTTGTTGG GCGAAGGTTT GGCCAGATTT GCCCAATATA 
ACAATAGATG AGAGTCTAAC TGAAGATGAA GCTGTGAATG CTTCCAGAGC TCATGTAGCT 
TTTCCAGACT TTTTCAGGAA TTCCACAGCA GAGTGGTGGG CAACAGAAAT TATAGATTTC 
TACAATAACC AGATGAAGTT TGATGGCTTG TGGATTGATA TGAATGAACC ATCAAGTTTT 
GTACATGGAA CAGTTTCTAA TCAATGCAGG AACACAGAGT TAAATTATCC ACCTTATCTC 
CCAGAACTCA CAAAAAGAAC TAGTGGATTA CATTTCAGAA CTATGTGTAT GGAAACTGAG 
CAGATTCTTA GTGATGGATC ATCTGTTTTG CATTACAATG TTCACAATCT ATATGGATGG 
TCACAAATGA AACCTAGTTA TGATGCATTA CAGAAGACAA CTGGCAAAAG AGGAATTGTT 
ATTTCTCGCT CCACCTATCC TTCTGGTGGA CGATGGGGAG GACATTGGCT TGGAGACAAT 
TATGCAAAAT GGGACAATCT GGACAAATCA ATCATTGGTA TGATGGAATT TAGTCTCTTT 
GGAATCTCAT ATACTGGAGC AGATATCTGT GGTTTCTTCA ACAATTCAGA ATATGAGCTC 
TGTGCCCGCT GGATGCAACT TGGAGCATTT TATCCATACT CAAGAAATCA CAACATTGCA 
AATACTAGGA GACAAGATCC TGCTTCCTGG AATTCAACTT TTTCTGAAAT GTCAAGGAAT 
ATTCTAAATA TTAGATATAC TTTATTGCCC TATTTCTATA CACAGATGCA TGAAATTCAT 
GTTAATGGTG GCACTGTTAT TCGACCCCTT TTGCATGAGT TCTTTAACGA CAGGATAACC 
TGGGATATAT TCAAGCAGTT CTTGTGGGGT CCTGCATTTA TGGTCACCCC TGTATTGGAG 
CCTCATGCTA ACACTGTACA AGGCTACGTC CCTGATGCCC GGTGGTTTGA TTATCATACG 
GGTCAAGATA TTGGTGTCAA AGGATTTGTT TTATTTAATG CTCCTTTAAA TACAATAAAC 
CTACATGTCC GTGGTGGTCA TATCCTACCA TGTCAAGAGC CTGCTCAAAA CACATTTCAC 
AGCCGACAAA ATTACATGAA GCTCATTGTT GCTGCAGATG TTAACCAGAG GGCACAGGGG 
TCTCTGTTTT GGGATGACGG AGAGAGTATA GATTCCTATG AAAGAGGCTT CTATTTCTCG 
GCACAATTTA ATTTAAACAA TAATACCTTG ACAAGCACTA TATTGCAAAA TGGTTACATA 
AACAAAGATG AAATGAGGCT TGGGTTCATC ATCATATGGG GGAAAGGAAA GACTCCTGTT 
ACTGGGGTTA ATCTCATATA TAATGGAAAT ACAGAAGCAG TTAAATTTAT TCAAGAAGCA 
GACAAGGAGA TATTAAATAT TGATCTGACA GTGAATAATG TTACTCTAGA TGAACCATTT 
CAAATCATCT GGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_545265.3
CDS	168..5645
Translation	MAKKKFTGLEISLIVLFVIVTIIAIALIAVLATKTPAVEAISNSTSTPPTTRTTTVYPGSGKCPSELNDPINERINCIPEQFPTQAVCAMRGCCWKPWNNSIIPWCFFVDNHGYNVGQLTATSTGLEATLNRISSPTLFGNDITRVLFTTQNQTANRFRFKITDPNNKRYEVPHQFVKEFTGTAASNTLYDVQVINNPFSIKVIRKSNGRILFDTSIGPLVYSDQYLQISTKLPSEYMYGIGEHIHKRFRHDLNWKTWPIFTRDQLPGDNNNNLYGHHTFFMCIEDETGKSFGVFLMNSNAMEIFIQPTPVVTYRVTGGILDFYIFLGDTPEQVVQQYQELIGRPAMPAYWSLGFQLSRWNYKSLDVVKEVVKRNRDAGIPFDTQVTDVDYMEAKKDFTYDKVAFQGLPEFVQDLHDHGQKYVIILDPAISIDKLANGAAYETYDRGNAKNVWVNDSDGTTAIIGEVWPGLTVFPDFTNPNCIDWWANECSIFYQEVKYDGLWIDMNEVSSFIQGSQKGCNNNKLNYPPFTPDILDKLLYSKTICMDAVQYWGKQYDVHSLYGYSMAIATEKAVEKVFPSKRSFILTRSTFAGSGHYAAHWLGDNTASWEQMEWSIAGMLEFSLFGMPLVGADICGFVVNTTEELCRRWMQLGAFYPFSRNHNADGYEHQDPAFFGQNSLLVNSSRHYLNIRYTLLPFLYTLFYKAHVFGETVARPVLHEFYDDRESWIEDTQFLWGPALLITPVLKEGTDTVSAYIPNATWYDYETGAKRPWKKQRVNMYLPGDKIGLHLRGGYIIPIQQPAVTTTASRKNPLGLIVALDDNNIAKGDFFWDDGETKNTVQNGNYILYTFSVSNNKLDIICTHSSYQEGTTLAFETIKILGLIDSVTQVTVVENNQPMKNHYNFTYTASNQSLLIYNLKLNLGGNFTVQWNQNFSENEKFNCYPDADTATKEKCEARGCLWETSFSSKAPECYFPRQSNPYLVRSTQYSSMGITTDLQLNPTSARIKLPSEPISTLRVEVKYHKNDMLQFKIYDPQTKRYEVPIPLNIPDVPTSTYENRLYDVEIRENPFGIQVRRRSTGRVIWDSQLPGFAFNDQFIQISTRLPSEYIYGFGEVEHTAFKRDLNWNTWGMFTRDQPPGYKLNSYGFHPYYMALEDEGYAHGVLLLNSNAMDVTFQPTPALTYRVIGGILDFYMFLGPTPEVATKQYHEVIGRPVMPPYWALGFQICRYGYRNTSQVQQVYDEMVAAQIPYDVQYTDIDYMERQLDFTIDENFRDLPAFVDKIRQEGMRYIIILDPAISGNETKYYSAFERGQEKDVFVKWPNTNDICWAKVWPDLPNITIDESLTEDEAVNASRAHVAFPDFFRNSTAEWWATEIIDFYNNQMKFDGLWIDMNEPSSFVHGTVSNQCRNTELNYPPYLPELTKRTSGLHFRTMCMETEQILSDGSSVLHYNVHNLYGWSQMKPSYDALQKTTGKRGIVISRSTYPSGGRWGGHWLGDNYAKWDNLDKSIIGMMEFSLFGISYTGADICGFFNNSEYELCARWMQLGAFYPYSRNHNIANTRRQDPASWNSTFSEMSRNILNIRYTLLPYFYTQMHEIHVNGGTVIRPLLHEFFNDRITWDIFKQFLWGPAFMVTPVLEPHANTVQGYVPDARWFDYHTGQDIGVKGFVLFNAPLNTINLHVRGGHILPCQEPAQNTFHSRQNYMKLIVAADVNQRAQGSLFWDDGESIDSYERGFYFSAQFNLNNNTLTSTILQNGYINKDEMRLGFIIIWGKGKTPVTGVNLIYNGNTEAVKFIQEADKEILNIDLTVNNVTLDEPFQIIWS

Target ORF information:

RefSeq Version	XM_545265.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_545265.5

ORF Insert Sequence:

ATGGCAAAGA AGAAATTTAC TGGATTAGAA ATCTCTCTGA TTGTCCTTTT TGTTATAGTT 
ACTATAATAG CTATTGCCCT AATTGCTGTT TTAGCAACTA AGACACCTGC TGTTGAAGCA 
ATTAGTAATT CTACTTCAAC TCCACCTACC ACTCGTACAA CCACTGTGTA TCCTGGTTCA 
GGAAAATGTC CAAGCGAGCT AAATGATCCT ATCAATGAGA GAATAAACTG CATTCCAGAA 
CAATTTCCAA CACAGGCAGT TTGTGCAATG CGAGGTTGCT GCTGGAAGCC ATGGAATAAC 
TCTATTATTC CTTGGTGCTT CTTCGTAGAT AACCATGGTT ATAATGTTGG ACAACTGACA 
GCAACAAGTA CTGGACTTGA AGCCACATTA AACAGGATAT CTTCACCTAC ATTGTTTGGA 
AATGACATTA CCCGTGTTCT CTTCACAACT CAAAACCAAA CAGCCAATCG TTTCCGGTTT 
AAGATTACTG ATCCAAATAA CAAAAGATAT GAAGTCCCTC ACCAGTTTGT AAAAGAATTT 
ACTGGAACTG CAGCTTCTAA TACATTGTAT GATGTACAGG TTATAAATAA TCCATTCAGC 
ATCAAAGTCA TTAGAAAAAG CAATGGGAGA ATTTTATTTG ATACCAGCAT TGGTCCCCTG 
GTTTACTCTG ACCAGTATTT ACAGATCTCA ACCAAACTTC CTAGTGAATA TATGTATGGT 
ATTGGGGAAC ATATTCATAA GAGATTTCGT CATGATTTAA ACTGGAAAAC ATGGCCAATT 
TTTACCCGGG ATCAACTTCC TGGTGATAAT AATAATAATT TATATGGCCA TCATACATTC 
TTCATGTGTA TTGAAGACGA GACTGGAAAA TCATTTGGTG TTTTCTTAAT GAACAGCAAT 
GCAATGGAAA TTTTCATCCA GCCTACTCCA GTAGTAACTT ACAGAGTTAC TGGTGGAATT 
CTGGACTTTT ACATCTTTCT AGGAGATACA CCAGAACAGG TAGTTCAACA GTATCAAGAG 
CTCATTGGAC GACCAGCAAT GCCAGCCTAT TGGAGTCTTG GATTCCAACT GAGTCGCTGG 
AATTATAAGT CACTTGATGT AGTGAAAGAA GTGGTAAAAA GAAATAGAGA TGCTGGCATA 
CCATTTGATA CACAAGTCAC TGATGTTGAC TATATGGAAG CCAAGAAAGA TTTTACTTAT 
GACAAAGTTG CATTTCAGGG GCTCCCTGAA TTTGTTCAAG ATTTACATGA CCATGGACAG 
AAATATGTCA TCATCTTGGA CCCTGCAATT TCCATAGATA AGCTTGCCAA TGGAGCAGCA 
TATGAAACCT ATGACAGGGG AAATGCAAAA AATGTGTGGG TAAATGATTC AGATGGGACT 
ACAGCAATTA TTGGAGAGGT ATGGCCAGGA TTAACAGTAT TCCCTGACTT TACTAATCCA 
AACTGCATAG ATTGGTGGGC AAATGAATGC AGCATTTTCT ATCAAGAAGT GAAATATGAT 
GGACTTTGGA TTGACATGAA TGAAGTTTCC AGCTTTATTC AAGGTTCACA GAAAGGATGC 
AATAACAACA AACTGAATTA TCCACCTTTT ACTCCTGATA TTCTTGACAA ACTCCTGTAT 
TCCAAAACTA TTTGCATGGA TGCTGTGCAG TACTGGGGGA AGCAGTATGA TGTTCACAGC 
CTCTATGGGT ACAGCATGGC TATAGCCACA GAGAAAGCTG TAGAAAAAGT TTTTCCTAGT 
AAGAGAAGCT TTATTCTCAC CCGGTCAACT TTTGCTGGAT CTGGGCACTA TGCTGCACAC 
TGGTTAGGAG ACAATACTGC TTCATGGGAA CAAATGGAAT GGTCTATTGC AGGAATGCTG 
GAGTTCAGTT TGTTTGGAAT GCCTTTGGTT GGAGCAGACA TCTGTGGATT TGTGGTTAAC 
ACCACAGAAG AGCTTTGCAG AAGGTGGATG CAACTTGGGG CATTTTATCC ATTTTCCAGG 
AACCATAATG CTGATGGATA TGAGCATCAG GATCCAGCAT TTTTTGGCCA GAATTCCCTT 
TTGGTTAATT CATCAAGGCA CTATTTGAAT ATTCGCTATA CCTTATTACC TTTCCTCTAT 
ACTCTGTTTT ACAAAGCCCA TGTGTTTGGA GAAACGGTAG CAAGGCCCGT TCTTCATGAG 
TTTTATGATG ACAGAGAAAG CTGGATTGAG GACACTCAGT TCTTATGGGG CCCTGCGTTA 
CTTATTACTC CCGTCTTAAA AGAGGGAACA GATACAGTGA GTGCATACAT CCCTAATGCT 
ACTTGGTATG ATTATGAAAC TGGTGCAAAA AGGCCATGGA AAAAACAGCG TGTTAATATG 
TATCTTCCGG GCGACAAAAT AGGATTACAT CTTAGAGGAG GTTATATTAT CCCCATTCAG 
CAACCTGCTG TAACTACAAC TGCAAGCCGA AAGAACCCTC TAGGACTTAT AGTTGCATTG 
GATGACAATA ACATAGCAAA AGGGGACTTT TTCTGGGATG ATGGAGAAAC TAAGAATACC 
GTACAAAATG GCAATTACAT TTTATATACT TTTTCAGTTT CCAATAACAA ATTAGATATT 
ATATGCACAC ATTCATCATA CCAGGAAGGA ACCACTTTAG CATTTGAGAC TATAAAAATC 
CTTGGGTTGA TAGATTCTGT TACACAAGTT ACAGTGGTGG AAAATAATCA GCCAATGAAG 
AATCACTACA ATTTTACTTA TACTGCTTCC AACCAGAGTC TCCTAATTTA CAATCTCAAG 
TTGAACCTTG GAGGAAACTT TACTGTTCAG TGGAATCAAA ATTTCTCAGA AAATGAAAAG 
TTTAATTGTT ACCCAGATGC AGACACTGCA ACTAAAGAAA AATGTGAAGC ACGTGGCTGT 
TTATGGGAAA CGTCTTTTAG CTCCAAAGCA CCAGAGTGTT ACTTTCCTAG ACAATCCAAC 
CCATATCTAG TCCGTTCAAC TCAGTATTCA TCAATGGGTA TAACAACTGA CCTGCAACTT 
AATCCTACAA GTGCTCGAAT AAAGCTACCT TCTGAACCCA TCTCAACTCT TCGTGTGGAA 
GTGAAATATC ACAAAAATGA TATGCTGCAG TTTAAGATTT ATGATCCCCA AACAAAGAGA 
TATGAAGTTC CAATACCTTT AAACATTCCA GACGTGCCAA CAAGTACCTA TGAAAATAGG 
CTTTATGATG TTGAAATCAG AGAAAATCCT TTTGGCATCC AGGTTCGAAG AAGAAGTACA 
GGAAGAGTTA TTTGGGATTC TCAGCTGCCA GGATTTGCTT TTAATGACCA GTTTATTCAA 
ATATCTACTC GCCTGCCATC AGAATATATA TATGGTTTTG GGGAAGTGGA ACATACAGCA 
TTTAAGAGAG ACCTGAACTG GAATACCTGG GGAATGTTCA CAAGAGACCA ACCCCCTGGT 
TATAAACTTA ATTCCTATGG ATTTCATCCC TATTACATGG CTCTGGAAGA CGAAGGCTAT 
GCTCATGGAG TTTTATTACT TAACAGCAAT GCAATGGATG TTACATTTCA GCCAACTCCT 
GCTCTAACTT ACCGTGTGAT TGGAGGAATC TTGGACTTTT ATATGTTTTT GGGCCCAACT 
CCAGAAGTTG CAACAAAGCA ATACCATGAA GTAATTGGCC GACCAGTCAT GCCACCTTAC 
TGGGCTTTGG GATTCCAAAT ATGTCGTTAT GGGTACAGAA ATACTTCACA AGTTCAGCAA 
GTATATGATG AGATGGTGGC TGCTCAGATC CCCTATGATG TTCAGTATAC AGACATTGAT 
TACATGGAAA GGCAACTAGA CTTTACAATT GATGAAAACT TCCGTGACCT TCCTGCGTTT 
GTTGACAAAA TAAGACAGGA AGGAATGAGA TACATTATTA TCCTGGATCC AGCAATTTCA 
GGAAATGAAA CAAAGTATTA CTCCGCATTT GAAAGAGGAC AGGAGAAAGA TGTTTTTGTC 
AAATGGCCTA ACACCAATGA TATTTGTTGG GCGAAGGTTT GGCCAGATTT GCCCAATATA 
ACAATAGATG AGAGTCTAAC TGAAGATGAA GCTGTGAATG CTTCCAGAGC TCATGTAGCT 
TTTCCAGACT TTTTCAGGAA TTCCACAGCA GAGTGGTGGG CAACAGAAAT TATAGATTTC 
TACAATAACC AGATGAAGTT TGATGGCTTG TGGATTGATA TGAATGAACC ATCAAGTTTT 
GTACATGGAA CAGTTTCTAA TCAATGCAGG AACACAGAGT TAAATTATCC ACCTTATCTC 
CCAGAACTCA CAAAAAGAAC TAGTGGATTA CATTTCAGAA CTATGTGTAT GGAAACTGAG 
CAGATTCTTA GTGATGGATC ATCTGTTTTG CATTACAATG TTCACAATCT ATATGGATGG 
TCACAAATGA AACCTAGTTA TGATGCATTA CAGAAGACAA CTGGCAAAAG AGGAATTGTT 
ATTTCTCGCT CCACCTATCC TTCTGGTGGA CGATGGGGAG GACATTGGCT TGGAGACAAT 
TATGCAAAAT GGGACAATCT GGACAAATCA ATCATTGGTA TGATGGAATT TAGTCTCTTT 
GGAATCTCAT ATACTGGAGC AGATATCTGT GGTTTCTTCA ACAATTCAGA ATATGAGCTC 
TGTGCCCGCT GGATGCAACT TGGAGCATTT TATCCATACT CAAGAAATCA CAACATTGCA 
AATACTAGGA GACAAGATCC TGCTTCCTGG AATTCAACTT TTTCTGAAAT GTCAAGGAAT 
ATTCTAAATA TTAGATATAC TTTATTGCCC TATTTCTATA CACAGATGCA TGAAATTCAT 
GTTAATGGTG GCACTGTTAT TCGACCCCTT TTGCATGAGT TCTTTAACGA CAGGATAACC 
TGGGATATAT TCAAGCAGTT CTTGTGGGGT CCTGCATTTA TGGTCACCCC TGTATTGGAG 
CCTCATGCTA ACACTGTACA AGGCTACGTC CCTGATGCCC GGTGGTTTGA TTATCATACG 
GGTCAAGATA TTGGTGTCAA AGGATTTGTT TTATTTAATG CTCCTTTAAA TACAATAAAC 
CTACATGTCC GTGGTGGTCA TATCCTACCA TGTCAAGAGC CTGCTCAAAA CACATTTCAC 
AGCCGACAAA ATTACATGAA GCTCATTGTT GCTGCAGATG TTAACCAGAG GGCACAGGGG 
TCTCTGTTTT GGGATGACGG AGAGAGTATA GATTCCTATG AAAGAGGCTT CTATTTCTCG 
GCACAATTTA ATTTAAACAA TAATACCTTG ACAAGCACTA TATTGCAAAA TGGTTACATA 
AACAAAGATG AAATGAGGCT TGGGTTCATC ATCATATGGG GGAAAGGAAA GACTCCTGTT 
ACTGGGGTTA ATCTCATATA TAATGGAAAT ACAGAAGCAG TTAAATTTAT TCAAGAAGCA 
GACAAGGAGA TATTAAATAT TGATCTGACA GTGAATAATG TTACTCTAGA TGAACCATTT 
CAAATCATCT GGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI ( XM_545265.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_545265.3 Canis lupus familiaris sucrase-isomaltase (SI), transcript variant X1, mRNA.

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CloneID	OCe30577
Clone ID Related Accession (Same CDS sequence)	XM_545265.5 , XM_545265.6
Accession Version	XM_545265.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	sucrase-isomaltase, intestinal isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_545265.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGA AGAAATTTAC TGGATTAGAA ATCTCTCTGA TTGTCCTTTT TGTTATAGTT 
ACTATAATAG CTATTGCCCT AATTGCTGTT TTAGCAACTA AGACACCTGC TGTTGAAGCA 
ATTAGTAATT CTACTTCAAC TCCACCTACC ACTCGTACAA CCACTGTGTA TCCTGGTTCA 
GGAAAATGTC CAAGCGAGCT AAATGATCCT ATCAATGAGA GAATAAACTG CATTCCAGAA 
CAATTTCCAA CACAGGCAGT TTGTGCAATG CGAGGTTGCT GCTGGAAGCC ATGGAATAAC 
TCTATTATTC CTTGGTGCTT CTTCGTAGAT AACCATGGTT ATAATGTTGG ACAACTGACA 
GCAACAAGTA CTGGACTTGA AGCCACATTA AACAGGATAT CTTCACCTAC ATTGTTTGGA 
AATGACATTA CCCGTGTTCT CTTCACAACT CAAAACCAAA CAGCCAATCG TTTCCGGTTT 
AAGATTACTG ATCCAAATAA CAAAAGATAT GAAGTCCCTC ACCAGTTTGT AAAAGAATTT 
ACTGGAACTG CAGCTTCTAA TACATTGTAT GATGTACAGG TTATAAATAA TCCATTCAGC 
ATCAAAGTCA TTAGAAAAAG CAATGGGAGA ATTTTATTTG ATACCAGCAT TGGTCCCCTG 
GTTTACTCTG ACCAGTATTT ACAGATCTCA ACCAAACTTC CTAGTGAATA TATGTATGGT 
ATTGGGGAAC ATATTCATAA GAGATTTCGT CATGATTTAA ACTGGAAAAC ATGGCCAATT 
TTTACCCGGG ATCAACTTCC TGGTGATAAT AATAATAATT TATATGGCCA TCATACATTC 
TTCATGTGTA TTGAAGACGA GACTGGAAAA TCATTTGGTG TTTTCTTAAT GAACAGCAAT 
GCAATGGAAA TTTTCATCCA GCCTACTCCA GTAGTAACTT ACAGAGTTAC TGGTGGAATT 
CTGGACTTTT ACATCTTTCT AGGAGATACA CCAGAACAGG TAGTTCAACA GTATCAAGAG 
CTCATTGGAC GACCAGCAAT GCCAGCCTAT TGGAGTCTTG GATTCCAACT GAGTCGCTGG 
AATTATAAGT CACTTGATGT AGTGAAAGAA GTGGTAAAAA GAAATAGAGA TGCTGGCATA 
CCATTTGATA CACAAGTCAC TGATGTTGAC TATATGGAAG CCAAGAAAGA TTTTACTTAT 
GACAAAGTTG CATTTCAGGG GCTCCCTGAA TTTGTTCAAG ATTTACATGA CCATGGACAG 
AAATATGTCA TCATCTTGGA CCCTGCAATT TCCATAGATA AGCTTGCCAA TGGAGCAGCA 
TATGAAACCT ATGACAGGGG AAATGCAAAA AATGTGTGGG TAAATGATTC AGATGGGACT 
ACAGCAATTA TTGGAGAGGT ATGGCCAGGA TTAACAGTAT TCCCTGACTT TACTAATCCA 
AACTGCATAG ATTGGTGGGC AAATGAATGC AGCATTTTCT ATCAAGAAGT GAAATATGAT 
GGACTTTGGA TTGACATGAA TGAAGTTTCC AGCTTTATTC AAGGTTCACA GAAAGGATGC 
AATAACAACA AACTGAATTA TCCACCTTTT ACTCCTGATA TTCTTGACAA ACTCCTGTAT 
TCCAAAACTA TTTGCATGGA TGCTGTGCAG TACTGGGGGA AGCAGTATGA TGTTCACAGC 
CTCTATGGGT ACAGCATGGC TATAGCCACA GAGAAAGCTG TAGAAAAAGT TTTTCCTAGT 
AAGAGAAGCT TTATTCTCAC CCGGTCAACT TTTGCTGGAT CTGGGCACTA TGCTGCACAC 
TGGTTAGGAG ACAATACTGC TTCATGGGAA CAAATGGAAT GGTCTATTGC AGGAATGCTG 
GAGTTCAGTT TGTTTGGAAT GCCTTTGGTT GGAGCAGACA TCTGTGGATT TGTGGTTAAC 
ACCACAGAAG AGCTTTGCAG AAGGTGGATG CAACTTGGGG CATTTTATCC ATTTTCCAGG 
AACCATAATG CTGATGGATA TGAGCATCAG GATCCAGCAT TTTTTGGCCA GAATTCCCTT 
TTGGTTAATT CATCAAGGCA CTATTTGAAT ATTCGCTATA CCTTATTACC TTTCCTCTAT 
ACTCTGTTTT ACAAAGCCCA TGTGTTTGGA GAAACGGTAG CAAGGCCCGT TCTTCATGAG 
TTTTATGATG ACAGAGAAAG CTGGATTGAG GACACTCAGT TCTTATGGGG CCCTGCGTTA 
CTTATTACTC CCGTCTTAAA AGAGGGAACA GATACAGTGA GTGCATACAT CCCTAATGCT 
ACTTGGTATG ATTATGAAAC TGGTGCAAAA AGGCCATGGA AAAAACAGCG TGTTAATATG 
TATCTTCCGG GCGACAAAAT AGGATTACAT CTTAGAGGAG GTTATATTAT CCCCATTCAG 
CAACCTGCTG TAACTACAAC TGCAAGCCGA AAGAACCCTC TAGGACTTAT AGTTGCATTG 
GATGACAATA ACATAGCAAA AGGGGACTTT TTCTGGGATG ATGGAGAAAC TAAGAATACC 
GTACAAAATG GCAATTACAT TTTATATACT TTTTCAGTTT CCAATAACAA ATTAGATATT 
ATATGCACAC ATTCATCATA CCAGGAAGGA ACCACTTTAG CATTTGAGAC TATAAAAATC 
CTTGGGTTGA TAGATTCTGT TACACAAGTT ACAGTGGTGG AAAATAATCA GCCAATGAAG 
AATCACTACA ATTTTACTTA TACTGCTTCC AACCAGAGTC TCCTAATTTA CAATCTCAAG 
TTGAACCTTG GAGGAAACTT TACTGTTCAG TGGAATCAAA ATTTCTCAGA AAATGAAAAG 
TTTAATTGTT ACCCAGATGC AGACACTGCA ACTAAAGAAA AATGTGAAGC ACGTGGCTGT 
TTATGGGAAA CGTCTTTTAG CTCCAAAGCA CCAGAGTGTT ACTTTCCTAG ACAATCCAAC 
CCATATCTAG TCCGTTCAAC TCAGTATTCA TCAATGGGTA TAACAACTGA CCTGCAACTT 
AATCCTACAA GTGCTCGAAT AAAGCTACCT TCTGAACCCA TCTCAACTCT TCGTGTGGAA 
GTGAAATATC ACAAAAATGA TATGCTGCAG TTTAAGATTT ATGATCCCCA AACAAAGAGA 
TATGAAGTTC CAATACCTTT AAACATTCCA GACGTGCCAA CAAGTACCTA TGAAAATAGG 
CTTTATGATG TTGAAATCAG AGAAAATCCT TTTGGCATCC AGGTTCGAAG AAGAAGTACA 
GGAAGAGTTA TTTGGGATTC TCAGCTGCCA GGATTTGCTT TTAATGACCA GTTTATTCAA 
ATATCTACTC GCCTGCCATC AGAATATATA TATGGTTTTG GGGAAGTGGA ACATACAGCA 
TTTAAGAGAG ACCTGAACTG GAATACCTGG GGAATGTTCA CAAGAGACCA ACCCCCTGGT 
TATAAACTTA ATTCCTATGG ATTTCATCCC TATTACATGG CTCTGGAAGA CGAAGGCTAT 
GCTCATGGAG TTTTATTACT TAACAGCAAT GCAATGGATG TTACATTTCA GCCAACTCCT 
GCTCTAACTT ACCGTGTGAT TGGAGGAATC TTGGACTTTT ATATGTTTTT GGGCCCAACT 
CCAGAAGTTG CAACAAAGCA ATACCATGAA GTAATTGGCC GACCAGTCAT GCCACCTTAC 
TGGGCTTTGG GATTCCAAAT ATGTCGTTAT GGGTACAGAA ATACTTCACA AGTTCAGCAA 
GTATATGATG AGATGGTGGC TGCTCAGATC CCCTATGATG TTCAGTATAC AGACATTGAT 
TACATGGAAA GGCAACTAGA CTTTACAATT GATGAAAACT TCCGTGACCT TCCTGCGTTT 
GTTGACAAAA TAAGACAGGA AGGAATGAGA TACATTATTA TCCTGGATCC AGCAATTTCA 
GGAAATGAAA CAAAGTATTA CTCCGCATTT GAAAGAGGAC AGGAGAAAGA TGTTTTTGTC 
AAATGGCCTA ACACCAATGA TATTTGTTGG GCGAAGGTTT GGCCAGATTT GCCCAATATA 
ACAATAGATG AGAGTCTAAC TGAAGATGAA GCTGTGAATG CTTCCAGAGC TCATGTAGCT 
TTTCCAGACT TTTTCAGGAA TTCCACAGCA GAGTGGTGGG CAACAGAAAT TATAGATTTC 
TACAATAACC AGATGAAGTT TGATGGCTTG TGGATTGATA TGAATGAACC ATCAAGTTTT 
GTACATGGAA CAGTTTCTAA TCAATGCAGG AACACAGAGT TAAATTATCC ACCTTATCTC 
CCAGAACTCA CAAAAAGAAC TAGTGGATTA CATTTCAGAA CTATGTGTAT GGAAACTGAG 
CAGATTCTTA GTGATGGATC ATCTGTTTTG CATTACAATG TTCACAATCT ATATGGATGG 
TCACAAATGA AACCTAGTTA TGATGCATTA CAGAAGACAA CTGGCAAAAG AGGAATTGTT 
ATTTCTCGCT CCACCTATCC TTCTGGTGGA CGATGGGGAG GACATTGGCT TGGAGACAAT 
TATGCAAAAT GGGACAATCT GGACAAATCA ATCATTGGTA TGATGGAATT TAGTCTCTTT 
GGAATCTCAT ATACTGGAGC AGATATCTGT GGTTTCTTCA ACAATTCAGA ATATGAGCTC 
TGTGCCCGCT GGATGCAACT TGGAGCATTT TATCCATACT CAAGAAATCA CAACATTGCA 
AATACTAGGA GACAAGATCC TGCTTCCTGG AATTCAACTT TTTCTGAAAT GTCAAGGAAT 
ATTCTAAATA TTAGATATAC TTTATTGCCC TATTTCTATA CACAGATGCA TGAAATTCAT 
GTTAATGGTG GCACTGTTAT TCGACCCCTT TTGCATGAGT TCTTTAACGA CAGGATAACC 
TGGGATATAT TCAAGCAGTT CTTGTGGGGT CCTGCATTTA TGGTCACCCC TGTATTGGAG 
CCTCATGCTA ACACTGTACA AGGCTACGTC CCTGATGCCC GGTGGTTTGA TTATCATACG 
GGTCAAGATA TTGGTGTCAA AGGATTTGTT TTATTTAATG CTCCTTTAAA TACAATAAAC 
CTACATGTCC GTGGTGGTCA TATCCTACCA TGTCAAGAGC CTGCTCAAAA CACATTTCAC 
AGCCGACAAA ATTACATGAA GCTCATTGTT GCTGCAGATG TTAACCAGAG GGCACAGGGG 
TCTCTGTTTT GGGATGACGG AGAGAGTATA GATTCCTATG AAAGAGGCTT CTATTTCTCG 
GCACAATTTA ATTTAAACAA TAATACCTTG ACAAGCACTA TATTGCAAAA TGGTTACATA 
AACAAAGATG AAATGAGGCT TGGGTTCATC ATCATATGGG GGAAAGGAAA GACTCCTGTT 
ACTGGGGTTA ATCTCATATA TAATGGAAAT ACAGAAGCAG TTAAATTTAT TCAAGAAGCA 
GACAAGGAGA TATTAAATAT TGATCTGACA GTGAATAATG TTACTCTAGA TGAACCATTT 
CAAATCATCT GGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_545265.3
CDS	167..5644
Translation	MAKKKFTGLEISLIVLFVIVTIIAIALIAVLATKTPAVEAISNSTSTPPTTRTTTVYPGSGKCPSELNDPINERINCIPEQFPTQAVCAMRGCCWKPWNNSIIPWCFFVDNHGYNVGQLTATSTGLEATLNRISSPTLFGNDITRVLFTTQNQTANRFRFKITDPNNKRYEVPHQFVKEFTGTAASNTLYDVQVINNPFSIKVIRKSNGRILFDTSIGPLVYSDQYLQISTKLPSEYMYGIGEHIHKRFRHDLNWKTWPIFTRDQLPGDNNNNLYGHHTFFMCIEDETGKSFGVFLMNSNAMEIFIQPTPVVTYRVTGGILDFYIFLGDTPEQVVQQYQELIGRPAMPAYWSLGFQLSRWNYKSLDVVKEVVKRNRDAGIPFDTQVTDVDYMEAKKDFTYDKVAFQGLPEFVQDLHDHGQKYVIILDPAISIDKLANGAAYETYDRGNAKNVWVNDSDGTTAIIGEVWPGLTVFPDFTNPNCIDWWANECSIFYQEVKYDGLWIDMNEVSSFIQGSQKGCNNNKLNYPPFTPDILDKLLYSKTICMDAVQYWGKQYDVHSLYGYSMAIATEKAVEKVFPSKRSFILTRSTFAGSGHYAAHWLGDNTASWEQMEWSIAGMLEFSLFGMPLVGADICGFVVNTTEELCRRWMQLGAFYPFSRNHNADGYEHQDPAFFGQNSLLVNSSRHYLNIRYTLLPFLYTLFYKAHVFGETVARPVLHEFYDDRESWIEDTQFLWGPALLITPVLKEGTDTVSAYIPNATWYDYETGAKRPWKKQRVNMYLPGDKIGLHLRGGYIIPIQQPAVTTTASRKNPLGLIVALDDNNIAKGDFFWDDGETKNTVQNGNYILYTFSVSNNKLDIICTHSSYQEGTTLAFETIKILGLIDSVTQVTVVENNQPMKNHYNFTYTASNQSLLIYNLKLNLGGNFTVQWNQNFSENEKFNCYPDADTATKEKCEARGCLWETSFSSKAPECYFPRQSNPYLVRSTQYSSMGITTDLQLNPTSARIKLPSEPISTLRVEVKYHKNDMLQFKIYDPQTKRYEVPIPLNIPDVPTSTYENRLYDVEIRENPFGIQVRRRSTGRVIWDSQLPGFAFNDQFIQISTRLPSEYIYGFGEVEHTAFKRDLNWNTWGMFTRDQPPGYKLNSYGFHPYYMALEDEGYAHGVLLLNSNAMDVTFQPTPALTYRVIGGILDFYMFLGPTPEVATKQYHEVIGRPVMPPYWALGFQICRYGYRNTSQVQQVYDEMVAAQIPYDVQYTDIDYMERQLDFTIDENFRDLPAFVDKIRQEGMRYIIILDPAISGNETKYYSAFERGQEKDVFVKWPNTNDICWAKVWPDLPNITIDESLTEDEAVNASRAHVAFPDFFRNSTAEWWATEIIDFYNNQMKFDGLWIDMNEPSSFVHGTVSNQCRNTELNYPPYLPELTKRTSGLHFRTMCMETEQILSDGSSVLHYNVHNLYGWSQMKPSYDALQKTTGKRGIVISRSTYPSGGRWGGHWLGDNYAKWDNLDKSIIGMMEFSLFGISYTGADICGFFNNSEYELCARWMQLGAFYPYSRNHNIANTRRQDPASWNSTFSEMSRNILNIRYTLLPYFYTQMHEIHVNGGTVIRPLLHEFFNDRITWDIFKQFLWGPAFMVTPVLEPHANTVQGYVPDARWFDYHTGQDIGVKGFVLFNAPLNTINLHVRGGHILPCQEPAQNTFHSRQNYMKLIVAADVNQRAQGSLFWDDGESIDSYERGFYFSAQFNLNNNTLTSTILQNGYINKDEMRLGFIIIWGKGKTPVTGVNLIYNGNTEAVKFIQEADKEILNIDLTVNNVTLDEPFQIIWS

Target ORF information:

RefSeq Version	XM_545265.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris sucrase-isomaltase (SI), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_545265.6

ORF Insert Sequence:

ATGGCAAAGA AGAAATTTAC TGGATTAGAA ATCTCTCTGA TTGTCCTTTT TGTTATAGTT 
ACTATAATAG CTATTGCCCT AATTGCTGTT TTAGCAACTA AGACACCTGC TGTTGAAGCA 
ATTAGTAATT CTACTTCAAC TCCACCTACC ACTCGTACAA CCACTGTGTA TCCTGGTTCA 
GGAAAATGTC CAAGCGAGCT AAATGATCCT ATCAATGAGA GAATAAACTG CATTCCAGAA 
CAATTTCCAA CACAGGCAGT TTGTGCAATG CGAGGTTGCT GCTGGAAGCC ATGGAATAAC 
TCTATTATTC CTTGGTGCTT CTTCGTAGAT AACCATGGTT ATAATGTTGG ACAACTGACA 
GCAACAAGTA CTGGACTTGA AGCCACATTA AACAGGATAT CTTCACCTAC ATTGTTTGGA 
AATGACATTA CCCGTGTTCT CTTCACAACT CAAAACCAAA CAGCCAATCG TTTCCGGTTT 
AAGATTACTG ATCCAAATAA CAAAAGATAT GAAGTCCCTC ACCAGTTTGT AAAAGAATTT 
ACTGGAACTG CAGCTTCTAA TACATTGTAT GATGTACAGG TTATAAATAA TCCATTCAGC 
ATCAAAGTCA TTAGAAAAAG CAATGGGAGA ATTTTATTTG ATACCAGCAT TGGTCCCCTG 
GTTTACTCTG ACCAGTATTT ACAGATCTCA ACCAAACTTC CTAGTGAATA TATGTATGGT 
ATTGGGGAAC ATATTCATAA GAGATTTCGT CATGATTTAA ACTGGAAAAC ATGGCCAATT 
TTTACCCGGG ATCAACTTCC TGGTGATAAT AATAATAATT TATATGGCCA TCATACATTC 
TTCATGTGTA TTGAAGACGA GACTGGAAAA TCATTTGGTG TTTTCTTAAT GAACAGCAAT 
GCAATGGAAA TTTTCATCCA GCCTACTCCA GTAGTAACTT ACAGAGTTAC TGGTGGAATT 
CTGGACTTTT ACATCTTTCT AGGAGATACA CCAGAACAGG TAGTTCAACA GTATCAAGAG 
CTCATTGGAC GACCAGCAAT GCCAGCCTAT TGGAGTCTTG GATTCCAACT GAGTCGCTGG 
AATTATAAGT CACTTGATGT AGTGAAAGAA GTGGTAAAAA GAAATAGAGA TGCTGGCATA 
CCATTTGATA CACAAGTCAC TGATGTTGAC TATATGGAAG CCAAGAAAGA TTTTACTTAT 
GACAAAGTTG CATTTCAGGG GCTCCCTGAA TTTGTTCAAG ATTTACATGA CCATGGACAG 
AAATATGTCA TCATCTTGGA CCCTGCAATT TCCATAGATA AGCTTGCCAA TGGAGCAGCA 
TATGAAACCT ATGACAGGGG AAATGCAAAA AATGTGTGGG TAAATGATTC AGATGGGACT 
ACAGCAATTA TTGGAGAGGT ATGGCCAGGA TTAACAGTAT TCCCTGACTT TACTAATCCA 
AACTGCATAG ATTGGTGGGC AAATGAATGC AGCATTTTCT ATCAAGAAGT GAAATATGAT 
GGACTTTGGA TTGACATGAA TGAAGTTTCC AGCTTTATTC AAGGTTCACA GAAAGGATGC 
AATAACAACA AACTGAATTA TCCACCTTTT ACTCCTGATA TTCTTGACAA ACTCCTGTAT 
TCCAAAACTA TTTGCATGGA TGCTGTGCAG TACTGGGGGA AGCAGTATGA TGTTCACAGC 
CTCTATGGGT ACAGCATGGC TATAGCCACA GAGAAAGCTG TAGAAAAAGT TTTTCCTAGT 
AAGAGAAGCT TTATTCTCAC CCGGTCAACT TTTGCTGGAT CTGGGCACTA TGCTGCACAC 
TGGTTAGGAG ACAATACTGC TTCATGGGAA CAAATGGAAT GGTCTATTGC AGGAATGCTG 
GAGTTCAGTT TGTTTGGAAT GCCTTTGGTT GGAGCAGACA TCTGTGGATT TGTGGTTAAC 
ACCACAGAAG AGCTTTGCAG AAGGTGGATG CAACTTGGGG CATTTTATCC ATTTTCCAGG 
AACCATAATG CTGATGGATA TGAGCATCAG GATCCAGCAT TTTTTGGCCA GAATTCCCTT 
TTGGTTAATT CATCAAGGCA CTATTTGAAT ATTCGCTATA CCTTATTACC TTTCCTCTAT 
ACTCTGTTTT ACAAAGCCCA TGTGTTTGGA GAAACGGTAG CAAGGCCCGT TCTTCATGAG 
TTTTATGATG ACAGAGAAAG CTGGATTGAG GACACTCAGT TCTTATGGGG CCCTGCGTTA 
CTTATTACTC CCGTCTTAAA AGAGGGAACA GATACAGTGA GTGCATACAT CCCTAATGCT 
ACTTGGTATG ATTATGAAAC TGGTGCAAAA AGGCCATGGA AAAAACAGCG TGTTAATATG 
TATCTTCCGG GCGACAAAAT AGGATTACAT CTTAGAGGAG GTTATATTAT CCCCATTCAG 
CAACCTGCTG TAACTACAAC TGCAAGCCGA AAGAACCCTC TAGGACTTAT AGTTGCATTG 
GATGACAATA ACATAGCAAA AGGGGACTTT TTCTGGGATG ATGGAGAAAC TAAGAATACC 
GTACAAAATG GCAATTACAT TTTATATACT TTTTCAGTTT CCAATAACAA ATTAGATATT 
ATATGCACAC ATTCATCATA CCAGGAAGGA ACCACTTTAG CATTTGAGAC TATAAAAATC 
CTTGGGTTGA TAGATTCTGT TACACAAGTT ACAGTGGTGG AAAATAATCA GCCAATGAAG 
AATCACTACA ATTTTACTTA TACTGCTTCC AACCAGAGTC TCCTAATTTA CAATCTCAAG 
TTGAACCTTG GAGGAAACTT TACTGTTCAG TGGAATCAAA ATTTCTCAGA AAATGAAAAG 
TTTAATTGTT ACCCAGATGC AGACACTGCA ACTAAAGAAA AATGTGAAGC ACGTGGCTGT 
TTATGGGAAA CGTCTTTTAG CTCCAAAGCA CCAGAGTGTT ACTTTCCTAG ACAATCCAAC 
CCATATCTAG TCCGTTCAAC TCAGTATTCA TCAATGGGTA TAACAACTGA CCTGCAACTT 
AATCCTACAA GTGCTCGAAT AAAGCTACCT TCTGAACCCA TCTCAACTCT TCGTGTGGAA 
GTGAAATATC ACAAAAATGA TATGCTGCAG TTTAAGATTT ATGATCCCCA AACAAAGAGA 
TATGAAGTTC CAATACCTTT AAACATTCCA GACGTGCCAA CAAGTACCTA TGAAAATAGG 
CTTTATGATG TTGAAATCAG AGAAAATCCT TTTGGCATCC AGGTTCGAAG AAGAAGTACA 
GGAAGAGTTA TTTGGGATTC TCAGCTGCCA GGATTTGCTT TTAATGACCA GTTTATTCAA 
ATATCTACTC GCCTGCCATC AGAATATATA TATGGTTTTG GGGAAGTGGA ACATACAGCA 
TTTAAGAGAG ACCTGAACTG GAATACCTGG GGAATGTTCA CAAGAGACCA ACCCCCTGGT 
TATAAACTTA ATTCCTATGG ATTTCATCCC TATTACATGG CTCTGGAAGA CGAAGGCTAT 
GCTCATGGAG TTTTATTACT TAACAGCAAT GCAATGGATG TTACATTTCA GCCAACTCCT 
GCTCTAACTT ACCGTGTGAT TGGAGGAATC TTGGACTTTT ATATGTTTTT GGGCCCAACT 
CCAGAAGTTG CAACAAAGCA ATACCATGAA GTAATTGGCC GACCAGTCAT GCCACCTTAC 
TGGGCTTTGG GATTCCAAAT ATGTCGTTAT GGGTACAGAA ATACTTCACA AGTTCAGCAA 
GTATATGATG AGATGGTGGC TGCTCAGATC CCCTATGATG TTCAGTATAC AGACATTGAT 
TACATGGAAA GGCAACTAGA CTTTACAATT GATGAAAACT TCCGTGACCT TCCTGCGTTT 
GTTGACAAAA TAAGACAGGA AGGAATGAGA TACATTATTA TCCTGGATCC AGCAATTTCA 
GGAAATGAAA CAAAGTATTA CTCCGCATTT GAAAGAGGAC AGGAGAAAGA TGTTTTTGTC 
AAATGGCCTA ACACCAATGA TATTTGTTGG GCGAAGGTTT GGCCAGATTT GCCCAATATA 
ACAATAGATG AGAGTCTAAC TGAAGATGAA GCTGTGAATG CTTCCAGAGC TCATGTAGCT 
TTTCCAGACT TTTTCAGGAA TTCCACAGCA GAGTGGTGGG CAACAGAAAT TATAGATTTC 
TACAATAACC AGATGAAGTT TGATGGCTTG TGGATTGATA TGAATGAACC ATCAAGTTTT 
GTACATGGAA CAGTTTCTAA TCAATGCAGG AACACAGAGT TAAATTATCC ACCTTATCTC 
CCAGAACTCA CAAAAAGAAC TAGTGGATTA CATTTCAGAA CTATGTGTAT GGAAACTGAG 
CAGATTCTTA GTGATGGATC ATCTGTTTTG CATTACAATG TTCACAATCT ATATGGATGG 
TCACAAATGA AACCTAGTTA TGATGCATTA CAGAAGACAA CTGGCAAAAG AGGAATTGTT 
ATTTCTCGCT CCACCTATCC TTCTGGTGGA CGATGGGGAG GACATTGGCT TGGAGACAAT 
TATGCAAAAT GGGACAATCT GGACAAATCA ATCATTGGTA TGATGGAATT TAGTCTCTTT 
GGAATCTCAT ATACTGGAGC AGATATCTGT GGTTTCTTCA ACAATTCAGA ATATGAGCTC 
TGTGCCCGCT GGATGCAACT TGGAGCATTT TATCCATACT CAAGAAATCA CAACATTGCA 
AATACTAGGA GACAAGATCC TGCTTCCTGG AATTCAACTT TTTCTGAAAT GTCAAGGAAT 
ATTCTAAATA TTAGATATAC TTTATTGCCC TATTTCTATA CACAGATGCA TGAAATTCAT 
GTTAATGGTG GCACTGTTAT TCGACCCCTT TTGCATGAGT TCTTTAACGA CAGGATAACC 
TGGGATATAT TCAAGCAGTT CTTGTGGGGT CCTGCATTTA TGGTCACCCC TGTATTGGAG 
CCTCATGCTA ACACTGTACA AGGCTACGTC CCTGATGCCC GGTGGTTTGA TTATCATACG 
GGTCAAGATA TTGGTGTCAA AGGATTTGTT TTATTTAATG CTCCTTTAAA TACAATAAAC 
CTACATGTCC GTGGTGGTCA TATCCTACCA TGTCAAGAGC CTGCTCAAAA CACATTTCAC 
AGCCGACAAA ATTACATGAA GCTCATTGTT GCTGCAGATG TTAACCAGAG GGCACAGGGG 
TCTCTGTTTT GGGATGACGG AGAGAGTATA GATTCCTATG AAAGAGGCTT CTATTTCTCG 
GCACAATTTA ATTTAAACAA TAATACCTTG ACAAGCACTA TATTGCAAAA TGGTTACATA 
AACAAAGATG AAATGAGGCT TGGGTTCATC ATCATATGGG GGAAAGGAAA GACTCCTGTT 
ACTGGGGTTA ATCTCATATA TAATGGAAAT ACAGAAGCAG TTAAATTTAT TCAAGAAGCA 
GACAAGGAGA TATTAAATAT TGATCTGACA GTGAATAATG TTACTCTAGA TGAACCATTT 
CAAATCATCT GGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI ( XM_005639880.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005639937.1 Canis lupus familiaris sucrase-isomaltase (SI), transcript variant X2, mRNA.

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CloneID	OCe30578
Clone ID Related Accession (Same CDS sequence)	XM_005639880.3 , XM_005639880.2
Accession Version	XM_005639880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	sucrase-isomaltase, intestinal isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005639880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGTT GCTGCTGGAA GCCATGGAAT AACTCTATTA TTCCTTGGTG CTTCTTCGTA 
GATAACCATG GTTATAATGT TGGACAACTG ACAGCAACAA GTACTGGACT TGAAGCCACA 
TTAAACAGGA TATCTTCACC TACATTGTTT GGAAATGACA TTACCCGTGT TCTCTTCACA 
ACTCAAAACC AAACAGCCAA TCGTTTCCGG TTTAAGATTA CTGATCCAAA TAACAAAAGA 
TATGAAGTCC CTCACCAGTT TGTAAAAGAA TTTACTGGAA CTGCAGCTTC TAATACATTG 
TATGATGTAC AGGTTATAAA TAATCCATTC AGCATCAAAG TCATTAGAAA AAGCAATGGG 
AGAATTTTAT TTGATACCAG CATTGGTCCC CTGGTTTACT CTGACCAGTA TTTACAGATC 
TCAACCAAAC TTCCTAGTGA ATATATGTAT GGTATTGGGG AACATATTCA TAAGAGATTT 
CGTCATGATT TAAACTGGAA AACATGGCCA ATTTTTACCC GGGATCAACT TCCTGGTGAT 
AATAATAATA ATTTATATGG CCATCATACA TTCTTCATGT GTATTGAAGA CGAGACTGGA 
AAATCATTTG GTGTTTTCTT AATGAACAGC AATGCAATGG AAATTTTCAT CCAGCCTACT 
CCAGTAGTAA CTTACAGAGT TACTGGTGGA ATTCTGGACT TTTACATCTT TCTAGGAGAT 
ACACCAGAAC AGGTAGTTCA ACAGTATCAA GAGCTCATTG GACGACCAGC AATGCCAGCC 
TATTGGAGTC TTGGATTCCA ACTGAGTCGC TGGAATTATA AGTCACTTGA TGTAGTGAAA 
GAAGTGGTAA AAAGAAATAG AGATGCTGGC ATACCATTTG ATACACAAGT CACTGATGTT 
GACTATATGG AAGCCAAGAA AGATTTTACT TATGACAAAG TTGCATTTCA GGGGCTCCCT 
GAATTTGTTC AAGATTTACA TGACCATGGA CAGAAATATG TCATCATCTT GGACCCTGCA 
ATTTCCATAG ATAAGCTTGC CAATGGAGCA GCATATGAAA CCTATGACAG GGGAAATGCA 
AAAAATGTGT GGGTAAATGA TTCAGATGGG ACTACAGCAA TTATTGGAGA GGTATGGCCA 
GGATTAACAG TATTCCCTGA CTTTACTAAT CCAAACTGCA TAGATTGGTG GGCAAATGAA 
TGCAGCATTT TCTATCAAGA AGTGAAATAT GATGGACTTT GGATTGACAT GAATGAAGTT 
TCCAGCTTTA TTCAAGGTTC ACAGAAAGGA TGCAATAACA ACAAACTGAA TTATCCACCT 
TTTACTCCTG ATATTCTTGA CAAACTCCTG TATTCCAAAA CTATTTGCAT GGATGCTGTG 
CAGTACTGGG GGAAGCAGTA TGATGTTCAC AGCCTCTATG GGTACAGCAT GGCTATAGCC 
ACAGAGAAAG CTGTAGAAAA AGTTTTTCCT AGTAAGAGAA GCTTTATTCT CACCCGGTCA 
ACTTTTGCTG GATCTGGGCA CTATGCTGCA CACTGGTTAG GAGACAATAC TGCTTCATGG 
GAACAAATGG AATGGTCTAT TGCAGGAATG CTGGAGTTCA GTTTGTTTGG AATGCCTTTG 
GTTGGAGCAG ACATCTGTGG ATTTGTGGTT AACACCACAG AAGAGCTTTG CAGAAGGTGG 
ATGCAACTTG GGGCATTTTA TCCATTTTCC AGGAACCATA ATGCTGATGG ATATGAGCAT 
CAGGATCCAG CATTTTTTGG CCAGAATTCC CTTTTGGTTA ATTCATCAAG GCACTATTTG 
AATATTCGCT ATACCTTATT ACCTTTCCTC TATACTCTGT TTTACAAAGC CCATGTGTTT 
GGAGAAACGG TAGCAAGGCC CGTTCTTCAT GAGTTTTATG ATGACAGAGA AAGCTGGATT 
GAGGACACTC AGTTCTTATG GGGCCCTGCG TTACTTATTA CTCCCGTCTT AAAAGAGGGA 
ACAGATACAG TGAGTGCATA CATCCCTAAT GCTACTTGGT ATGATTATGA AACTGGTGCA 
AAAAGGCCAT GGAAAAAACA GCGTGTTAAT ATGTATCTTC CGGGCGACAA AATAGGATTA 
CATCTTAGAG GAGGTTATAT TATCCCCATT CAGCAACCTG CTGTAACTAC AACTGCAAGC 
CGAAAGAACC CTCTAGGACT TATAGTTGCA TTGGATGACA ATAACATAGC AAAAGGGGAC 
TTTTTCTGGG ATGATGGAGA AACTAAGAAT ACCGTACAAA ATGGCAATTA CATTTTATAT 
ACTTTTTCAG TTTCCAATAA CAAATTAGAT ATTATATGCA CACATTCATC ATACCAGGAA 
GGAACCACTT TAGCATTTGA GACTATAAAA ATCCTTGGGT TGATAGATTC TGTTACACAA 
GTTACAGTGG TGGAAAATAA TCAGCCAATG AAGAATCACT ACAATTTTAC TTATACTGCT 
TCCAACCAGA GTCTCCTAAT TTACAATCTC AAGTTGAACC TTGGAGGAAA CTTTACTGTT 
CAGTGGAATC AAAATTTCTC AGAAAATGAA AAGTTTAATT GTTACCCAGA TGCAGACACT 
GCAACTAAAG AAAAATGTGA AGCACGTGGC TGTTTATGGG AAACGTCTTT TAGCTCCAAA 
GCACCAGAGT GTTACTTTCC TAGACAATCC AACCCATATC TAGTCCGTTC AACTCAGTAT 
TCATCAATGG GTATAACAAC TGACCTGCAA CTTAATCCTA CAAGTGCTCG AATAAAGCTA 
CCTTCTGAAC CCATCTCAAC TCTTCGTGTG GAAGTGAAAT ATCACAAAAA TGATATGCTG 
CAGTTTAAGA TTTATGATCC CCAAACAAAG AGATATGAAG TTCCAATACC TTTAAACATT 
CCAGACGTGC CAACAAGTAC CTATGAAAAT AGGCTTTATG ATGTTGAAAT CAGAGAAAAT 
CCTTTTGGCA TCCAGGTTCG AAGAAGAAGT ACAGGAAGAG TTATTTGGGA TTCTCAGCTG 
CCAGGATTTG CTTTTAATGA CCAGTTTATT CAAATATCTA CTCGCCTGCC ATCAGAATAT 
ATATATGGTT TTGGGGAAGT GGAACATACA GCATTTAAGA GAGACCTGAA CTGGAATACC 
TGGGGAATGT TCACAAGAGA CCAACCCCCT GGTTATAAAC TTAATTCCTA TGGATTTCAT 
CCCTATTACA TGGCTCTGGA AGACGAAGGC TATGCTCATG GAGTTTTATT ACTTAACAGC 
AATGCAATGG ATGTTACATT TCAGCCAACT CCTGCTCTAA CTTACCGTGT GATTGGAGGA 
ATCTTGGACT TTTATATGTT TTTGGGCCCA ACTCCAGAAG TTGCAACAAA GCAATACCAT 
GAAGTAATTG GCCGACCAGT CATGCCACCT TACTGGGCTT TGGGATTCCA AATATGTCGT 
TATGGGTACA GAAATACTTC ACAAGTTCAG CAAGTATATG ATGAGATGGT GGCTGCTCAG 
ATCCCCTATG ATGTTCAGTA TACAGACATT GATTACATGG AAAGGCAACT AGACTTTACA 
ATTGATGAAA ACTTCCGTGA CCTTCCTGCG TTTGTTGACA AAATAAGACA GGAAGGAATG 
AGATACATTA TTATCCTGGA TCCAGCAATT TCAGGAAATG AAACAAAGTA TTACTCCGCA 
TTTGAAAGAG GACAGGAGAA AGATGTTTTT GTCAAATGGC CTAACACCAA TGATATTTGT 
TGGGCGAAGG TTTGGCCAGA TTTGCCCAAT ATAACAATAG ATGAGAGTCT AACTGAAGAT 
GAAGCTGTGA ATGCTTCCAG AGCTCATGTA GCTTTTCCAG ACTTTTTCAG GAATTCCACA 
GCAGAGTGGT GGGCAACAGA AATTATAGAT TTCTACAATA ACCAGATGAA GTTTGATGGC 
TTGTGGATTG ATATGAATGA ACCATCAAGT TTTGTACATG GAACAGTTTC TAATCAATGC 
AGGAACACAG AGTTAAATTA TCCACCTTAT CTCCCAGAAC TCACAAAAAG AACTAGTGGA 
TTACATTTCA GAACTATGTG TATGGAAACT GAGCAGATTC TTAGTGATGG ATCATCTGTT 
TTGCATTACA ATGTTCACAA TCTATATGGA TGGTCACAAA TGAAACCTAG TTATGATGCA 
TTACAGAAGA CAACTGGCAA AAGAGGAATT GTTATTTCTC GCTCCACCTA TCCTTCTGGT 
GGACGATGGG GAGGACATTG GCTTGGAGAC AATTATGCAA AATGGGACAA TCTGGACAAA 
TCAATCATTG GTATGATGGA ATTTAGTCTC TTTGGAATCT CATATACTGG AGCAGATATC 
TGTGGTTTCT TCAACAATTC AGAATATGAG CTCTGTGCCC GCTGGATGCA ACTTGGAGCA 
TTTTATCCAT ACTCAAGAAA TCACAACATT GCAAATACTA GGAGACAAGA TCCTGCTTCC 
TGGAATTCAA CTTTTTCTGA AATGTCAAGG AATATTCTAA ATATTAGATA TACTTTATTG 
CCCTATTTCT ATACACAGAT GCATGAAATT CATGTTAATG GTGGCACTGT TATTCGACCC 
CTTTTGCATG AGTTCTTTAA CGACAGGATA ACCTGGGATA TATTCAAGCA GTTCTTGTGG 
GGTCCTGCAT TTATGGTCAC CCCTGTATTG GAGCCTCATG CTAACACTGT ACAAGGCTAC 
GTCCCTGATG CCCGGTGGTT TGATTATCAT ACGGGTCAAG ATATTGGTGT CAAAGGATTT 
GTTTTATTTA ATGCTCCTTT AAATACAATA AACCTACATG TCCGTGGTGG TCATATCCTA 
CCATGTCAAG AGCCTGCTCA AAACACATTT CACAGCCGAC AAAATTACAT GAAGCTCATT 
GTTGCTGCAG ATGTTAACCA GAGGGCACAG GGGTCTCTGT TTTGGGATGA CGGAGAGAGT 
ATAGATTCCT ATGAAAGAGG CTTCTATTTC TCGGCACAAT TTAATTTAAA CAATAATACC 
TTGACAAGCA CTATATTGCA AAATGGTTAC ATAAACAAAG ATGAAATGAG GCTTGGGTTC 
ATCATCATAT GGGGGAAAGG AAAGACTCCT GTTACTGGGG TTAATCTCAT ATATAATGGA 
AATACAGAAG CAGTTAAATT TATTCAAGAA GCAGACAAGG AGATATTAAA TATTGATCTG 
ACAGTGAATA ATGTTACTCT AGATGAACCA TTTCAAATCA TCTGGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005639937.1
CDS	366..5576
Translation	MRGCCWKPWNNSIIPWCFFVDNHGYNVGQLTATSTGLEATLNRISSPTLFGNDITRVLFTTQNQTANRFRFKITDPNNKRYEVPHQFVKEFTGTAASNTLYDVQVINNPFSIKVIRKSNGRILFDTSIGPLVYSDQYLQISTKLPSEYMYGIGEHIHKRFRHDLNWKTWPIFTRDQLPGDNNNNLYGHHTFFMCIEDETGKSFGVFLMNSNAMEIFIQPTPVVTYRVTGGILDFYIFLGDTPEQVVQQYQELIGRPAMPAYWSLGFQLSRWNYKSLDVVKEVVKRNRDAGIPFDTQVTDVDYMEAKKDFTYDKVAFQGLPEFVQDLHDHGQKYVIILDPAISIDKLANGAAYETYDRGNAKNVWVNDSDGTTAIIGEVWPGLTVFPDFTNPNCIDWWANECSIFYQEVKYDGLWIDMNEVSSFIQGSQKGCNNNKLNYPPFTPDILDKLLYSKTICMDAVQYWGKQYDVHSLYGYSMAIATEKAVEKVFPSKRSFILTRSTFAGSGHYAAHWLGDNTASWEQMEWSIAGMLEFSLFGMPLVGADICGFVVNTTEELCRRWMQLGAFYPFSRNHNADGYEHQDPAFFGQNSLLVNSSRHYLNIRYTLLPFLYTLFYKAHVFGETVARPVLHEFYDDRESWIEDTQFLWGPALLITPVLKEGTDTVSAYIPNATWYDYETGAKRPWKKQRVNMYLPGDKIGLHLRGGYIIPIQQPAVTTTASRKNPLGLIVALDDNNIAKGDFFWDDGETKNTVQNGNYILYTFSVSNNKLDIICTHSSYQEGTTLAFETIKILGLIDSVTQVTVVENNQPMKNHYNFTYTASNQSLLIYNLKLNLGGNFTVQWNQNFSENEKFNCYPDADTATKEKCEARGCLWETSFSSKAPECYFPRQSNPYLVRSTQYSSMGITTDLQLNPTSARIKLPSEPISTLRVEVKYHKNDMLQFKIYDPQTKRYEVPIPLNIPDVPTSTYENRLYDVEIRENPFGIQVRRRSTGRVIWDSQLPGFAFNDQFIQISTRLPSEYIYGFGEVEHTAFKRDLNWNTWGMFTRDQPPGYKLNSYGFHPYYMALEDEGYAHGVLLLNSNAMDVTFQPTPALTYRVIGGILDFYMFLGPTPEVATKQYHEVIGRPVMPPYWALGFQICRYGYRNTSQVQQVYDEMVAAQIPYDVQYTDIDYMERQLDFTIDENFRDLPAFVDKIRQEGMRYIIILDPAISGNETKYYSAFERGQEKDVFVKWPNTNDICWAKVWPDLPNITIDESLTEDEAVNASRAHVAFPDFFRNSTAEWWATEIIDFYNNQMKFDGLWIDMNEPSSFVHGTVSNQCRNTELNYPPYLPELTKRTSGLHFRTMCMETEQILSDGSSVLHYNVHNLYGWSQMKPSYDALQKTTGKRGIVISRSTYPSGGRWGGHWLGDNYAKWDNLDKSIIGMMEFSLFGISYTGADICGFFNNSEYELCARWMQLGAFYPYSRNHNIANTRRQDPASWNSTFSEMSRNILNIRYTLLPYFYTQMHEIHVNGGTVIRPLLHEFFNDRITWDIFKQFLWGPAFMVTPVLEPHANTVQGYVPDARWFDYHTGQDIGVKGFVLFNAPLNTINLHVRGGHILPCQEPAQNTFHSRQNYMKLIVAADVNQRAQGSLFWDDGESIDSYERGFYFSAQFNLNNNTLTSTILQNGYINKDEMRLGFIIIWGKGKTPVTGVNLIYNGNTEAVKFIQEADKEILNIDLTVNNVTLDEPFQIIWS

Target ORF information:

RefSeq Version	XM_005639880.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris sucrase-isomaltase (SI), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005639880.3

ORF Insert Sequence:

ATGCGAGGTT GCTGCTGGAA GCCATGGAAT AACTCTATTA TTCCTTGGTG CTTCTTCGTA 
GATAACCATG GTTATAATGT TGGACAACTG ACAGCAACAA GTACTGGACT TGAAGCCACA 
TTAAACAGGA TATCTTCACC TACATTGTTT GGAAATGACA TTACCCGTGT TCTCTTCACA 
ACTCAAAACC AAACAGCCAA TCGTTTCCGG TTTAAGATTA CTGATCCAAA TAACAAAAGA 
TATGAAGTCC CTCACCAGTT TGTAAAAGAA TTTACTGGAA CTGCAGCTTC TAATACATTG 
TATGATGTAC AGGTTATAAA TAATCCATTC AGCATCAAAG TCATTAGAAA AAGCAATGGG 
AGAATTTTAT TTGATACCAG CATTGGTCCC CTGGTTTACT CTGACCAGTA TTTACAGATC 
TCAACCAAAC TTCCTAGTGA ATATATGTAT GGTATTGGGG AACATATTCA TAAGAGATTT 
CGTCATGATT TAAACTGGAA AACATGGCCA ATTTTTACCC GGGATCAACT TCCTGGTGAT 
AATAATAATA ATTTATATGG CCATCATACA TTCTTCATGT GTATTGAAGA CGAGACTGGA 
AAATCATTTG GTGTTTTCTT AATGAACAGC AATGCAATGG AAATTTTCAT CCAGCCTACT 
CCAGTAGTAA CTTACAGAGT TACTGGTGGA ATTCTGGACT TTTACATCTT TCTAGGAGAT 
ACACCAGAAC AGGTAGTTCA ACAGTATCAA GAGCTCATTG GACGACCAGC AATGCCAGCC 
TATTGGAGTC TTGGATTCCA ACTGAGTCGC TGGAATTATA AGTCACTTGA TGTAGTGAAA 
GAAGTGGTAA AAAGAAATAG AGATGCTGGC ATACCATTTG ATACACAAGT CACTGATGTT 
GACTATATGG AAGCCAAGAA AGATTTTACT TATGACAAAG TTGCATTTCA GGGGCTCCCT 
GAATTTGTTC AAGATTTACA TGACCATGGA CAGAAATATG TCATCATCTT GGACCCTGCA 
ATTTCCATAG ATAAGCTTGC CAATGGAGCA GCATATGAAA CCTATGACAG GGGAAATGCA 
AAAAATGTGT GGGTAAATGA TTCAGATGGG ACTACAGCAA TTATTGGAGA GGTATGGCCA 
GGATTAACAG TATTCCCTGA CTTTACTAAT CCAAACTGCA TAGATTGGTG GGCAAATGAA 
TGCAGCATTT TCTATCAAGA AGTGAAATAT GATGGACTTT GGATTGACAT GAATGAAGTT 
TCCAGCTTTA TTCAAGGTTC ACAGAAAGGA TGCAATAACA ACAAACTGAA TTATCCACCT 
TTTACTCCTG ATATTCTTGA CAAACTCCTG TATTCCAAAA CTATTTGCAT GGATGCTGTG 
CAGTACTGGG GGAAGCAGTA TGATGTTCAC AGCCTCTATG GGTACAGCAT GGCTATAGCC 
ACAGAGAAAG CTGTAGAAAA AGTTTTTCCT AGTAAGAGAA GCTTTATTCT CACCCGGTCA 
ACTTTTGCTG GATCTGGGCA CTATGCTGCA CACTGGTTAG GAGACAATAC TGCTTCATGG 
GAACAAATGG AATGGTCTAT TGCAGGAATG CTGGAGTTCA GTTTGTTTGG AATGCCTTTG 
GTTGGAGCAG ACATCTGTGG ATTTGTGGTT AACACCACAG AAGAGCTTTG CAGAAGGTGG 
ATGCAACTTG GGGCATTTTA TCCATTTTCC AGGAACCATA ATGCTGATGG ATATGAGCAT 
CAGGATCCAG CATTTTTTGG CCAGAATTCC CTTTTGGTTA ATTCATCAAG GCACTATTTG 
AATATTCGCT ATACCTTATT ACCTTTCCTC TATACTCTGT TTTACAAAGC CCATGTGTTT 
GGAGAAACGG TAGCAAGGCC CGTTCTTCAT GAGTTTTATG ATGACAGAGA AAGCTGGATT 
GAGGACACTC AGTTCTTATG GGGCCCTGCG TTACTTATTA CTCCCGTCTT AAAAGAGGGA 
ACAGATACAG TGAGTGCATA CATCCCTAAT GCTACTTGGT ATGATTATGA AACTGGTGCA 
AAAAGGCCAT GGAAAAAACA GCGTGTTAAT ATGTATCTTC CGGGCGACAA AATAGGATTA 
CATCTTAGAG GAGGTTATAT TATCCCCATT CAGCAACCTG CTGTAACTAC AACTGCAAGC 
CGAAAGAACC CTCTAGGACT TATAGTTGCA TTGGATGACA ATAACATAGC AAAAGGGGAC 
TTTTTCTGGG ATGATGGAGA AACTAAGAAT ACCGTACAAA ATGGCAATTA CATTTTATAT 
ACTTTTTCAG TTTCCAATAA CAAATTAGAT ATTATATGCA CACATTCATC ATACCAGGAA 
GGAACCACTT TAGCATTTGA GACTATAAAA ATCCTTGGGT TGATAGATTC TGTTACACAA 
GTTACAGTGG TGGAAAATAA TCAGCCAATG AAGAATCACT ACAATTTTAC TTATACTGCT 
TCCAACCAGA GTCTCCTAAT TTACAATCTC AAGTTGAACC TTGGAGGAAA CTTTACTGTT 
CAGTGGAATC AAAATTTCTC AGAAAATGAA AAGTTTAATT GTTACCCAGA TGCAGACACT 
GCAACTAAAG AAAAATGTGA AGCACGTGGC TGTTTATGGG AAACGTCTTT TAGCTCCAAA 
GCACCAGAGT GTTACTTTCC TAGACAATCC AACCCATATC TAGTCCGTTC AACTCAGTAT 
TCATCAATGG GTATAACAAC TGACCTGCAA CTTAATCCTA CAAGTGCTCG AATAAAGCTA 
CCTTCTGAAC CCATCTCAAC TCTTCGTGTG GAAGTGAAAT ATCACAAAAA TGATATGCTG 
CAGTTTAAGA TTTATGATCC CCAAACAAAG AGATATGAAG TTCCAATACC TTTAAACATT 
CCAGACGTGC CAACAAGTAC CTATGAAAAT AGGCTTTATG ATGTTGAAAT CAGAGAAAAT 
CCTTTTGGCA TCCAGGTTCG AAGAAGAAGT ACAGGAAGAG TTATTTGGGA TTCTCAGCTG 
CCAGGATTTG CTTTTAATGA CCAGTTTATT CAAATATCTA CTCGCCTGCC ATCAGAATAT 
ATATATGGTT TTGGGGAAGT GGAACATACA GCATTTAAGA GAGACCTGAA CTGGAATACC 
TGGGGAATGT TCACAAGAGA CCAACCCCCT GGTTATAAAC TTAATTCCTA TGGATTTCAT 
CCCTATTACA TGGCTCTGGA AGACGAAGGC TATGCTCATG GAGTTTTATT ACTTAACAGC 
AATGCAATGG ATGTTACATT TCAGCCAACT CCTGCTCTAA CTTACCGTGT GATTGGAGGA 
ATCTTGGACT TTTATATGTT TTTGGGCCCA ACTCCAGAAG TTGCAACAAA GCAATACCAT 
GAAGTAATTG GCCGACCAGT CATGCCACCT TACTGGGCTT TGGGATTCCA AATATGTCGT 
TATGGGTACA GAAATACTTC ACAAGTTCAG CAAGTATATG ATGAGATGGT GGCTGCTCAG 
ATCCCCTATG ATGTTCAGTA TACAGACATT GATTACATGG AAAGGCAACT AGACTTTACA 
ATTGATGAAA ACTTCCGTGA CCTTCCTGCG TTTGTTGACA AAATAAGACA GGAAGGAATG 
AGATACATTA TTATCCTGGA TCCAGCAATT TCAGGAAATG AAACAAAGTA TTACTCCGCA 
TTTGAAAGAG GACAGGAGAA AGATGTTTTT GTCAAATGGC CTAACACCAA TGATATTTGT 
TGGGCGAAGG TTTGGCCAGA TTTGCCCAAT ATAACAATAG ATGAGAGTCT AACTGAAGAT 
GAAGCTGTGA ATGCTTCCAG AGCTCATGTA GCTTTTCCAG ACTTTTTCAG GAATTCCACA 
GCAGAGTGGT GGGCAACAGA AATTATAGAT TTCTACAATA ACCAGATGAA GTTTGATGGC 
TTGTGGATTG ATATGAATGA ACCATCAAGT TTTGTACATG GAACAGTTTC TAATCAATGC 
AGGAACACAG AGTTAAATTA TCCACCTTAT CTCCCAGAAC TCACAAAAAG AACTAGTGGA 
TTACATTTCA GAACTATGTG TATGGAAACT GAGCAGATTC TTAGTGATGG ATCATCTGTT 
TTGCATTACA ATGTTCACAA TCTATATGGA TGGTCACAAA TGAAACCTAG TTATGATGCA 
TTACAGAAGA CAACTGGCAA AAGAGGAATT GTTATTTCTC GCTCCACCTA TCCTTCTGGT 
GGACGATGGG GAGGACATTG GCTTGGAGAC AATTATGCAA AATGGGACAA TCTGGACAAA 
TCAATCATTG GTATGATGGA ATTTAGTCTC TTTGGAATCT CATATACTGG AGCAGATATC 
TGTGGTTTCT TCAACAATTC AGAATATGAG CTCTGTGCCC GCTGGATGCA ACTTGGAGCA 
TTTTATCCAT ACTCAAGAAA TCACAACATT GCAAATACTA GGAGACAAGA TCCTGCTTCC 
TGGAATTCAA CTTTTTCTGA AATGTCAAGG AATATTCTAA ATATTAGATA TACTTTATTG 
CCCTATTTCT ATACACAGAT GCATGAAATT CATGTTAATG GTGGCACTGT TATTCGACCC 
CTTTTGCATG AGTTCTTTAA CGACAGGATA ACCTGGGATA TATTCAAGCA GTTCTTGTGG 
GGTCCTGCAT TTATGGTCAC CCCTGTATTG GAGCCTCATG CTAACACTGT ACAAGGCTAC 
GTCCCTGATG CCCGGTGGTT TGATTATCAT ACGGGTCAAG ATATTGGTGT CAAAGGATTT 
GTTTTATTTA ATGCTCCTTT AAATACAATA AACCTACATG TCCGTGGTGG TCATATCCTA 
CCATGTCAAG AGCCTGCTCA AAACACATTT CACAGCCGAC AAAATTACAT GAAGCTCATT 
GTTGCTGCAG ATGTTAACCA GAGGGCACAG GGGTCTCTGT TTTGGGATGA CGGAGAGAGT 
ATAGATTCCT ATGAAAGAGG CTTCTATTTC TCGGCACAAT TTAATTTAAA CAATAATACC 
TTGACAAGCA CTATATTGCA AAATGGTTAC ATAAACAAAG ATGAAATGAG GCTTGGGTTC 
ATCATCATAT GGGGGAAAGG AAAGACTCCT GTTACTGGGG TTAATCTCAT ATATAATGGA 
AATACAGAAG CAGTTAAATT TATTCAAGAA GCAGACAAGG AGATATTAAA TATTGATCTG 
ACAGTGAATA ATGTTACTCT AGATGAACCA TTTCAAATCA TCTGGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI ( XM_005639880.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005639937.1 Canis lupus familiaris sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X2, mRNA.

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CloneID	OCe30578
Clone ID Related Accession (Same CDS sequence)	XM_005639880.3 , XM_005639880.2
Accession Version	XM_005639880.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	sucrase-isomaltase, intestinal isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGTT GCTGCTGGAA GCCATGGAAT AACTCTATTA TTCCTTGGTG CTTCTTCGTA 
GATAACCATG GTTATAATGT TGGACAACTG ACAGCAACAA GTACTGGACT TGAAGCCACA 
TTAAACAGGA TATCTTCACC TACATTGTTT GGAAATGACA TTACCCGTGT TCTCTTCACA 
ACTCAAAACC AAACAGCCAA TCGTTTCCGG TTTAAGATTA CTGATCCAAA TAACAAAAGA 
TATGAAGTCC CTCACCAGTT TGTAAAAGAA TTTACTGGAA CTGCAGCTTC TAATACATTG 
TATGATGTAC AGGTTATAAA TAATCCATTC AGCATCAAAG TCATTAGAAA AAGCAATGGG 
AGAATTTTAT TTGATACCAG CATTGGTCCC CTGGTTTACT CTGACCAGTA TTTACAGATC 
TCAACCAAAC TTCCTAGTGA ATATATGTAT GGTATTGGGG AACATATTCA TAAGAGATTT 
CGTCATGATT TAAACTGGAA AACATGGCCA ATTTTTACCC GGGATCAACT TCCTGGTGAT 
AATAATAATA ATTTATATGG CCATCATACA TTCTTCATGT GTATTGAAGA CGAGACTGGA 
AAATCATTTG GTGTTTTCTT AATGAACAGC AATGCAATGG AAATTTTCAT CCAGCCTACT 
CCAGTAGTAA CTTACAGAGT TACTGGTGGA ATTCTGGACT TTTACATCTT TCTAGGAGAT 
ACACCAGAAC AGGTAGTTCA ACAGTATCAA GAGCTCATTG GACGACCAGC AATGCCAGCC 
TATTGGAGTC TTGGATTCCA ACTGAGTCGC TGGAATTATA AGTCACTTGA TGTAGTGAAA 
GAAGTGGTAA AAAGAAATAG AGATGCTGGC ATACCATTTG ATACACAAGT CACTGATGTT 
GACTATATGG AAGCCAAGAA AGATTTTACT TATGACAAAG TTGCATTTCA GGGGCTCCCT 
GAATTTGTTC AAGATTTACA TGACCATGGA CAGAAATATG TCATCATCTT GGACCCTGCA 
ATTTCCATAG ATAAGCTTGC CAATGGAGCA GCATATGAAA CCTATGACAG GGGAAATGCA 
AAAAATGTGT GGGTAAATGA TTCAGATGGG ACTACAGCAA TTATTGGAGA GGTATGGCCA 
GGATTAACAG TATTCCCTGA CTTTACTAAT CCAAACTGCA TAGATTGGTG GGCAAATGAA 
TGCAGCATTT TCTATCAAGA AGTGAAATAT GATGGACTTT GGATTGACAT GAATGAAGTT 
TCCAGCTTTA TTCAAGGTTC ACAGAAAGGA TGCAATAACA ACAAACTGAA TTATCCACCT 
TTTACTCCTG ATATTCTTGA CAAACTCCTG TATTCCAAAA CTATTTGCAT GGATGCTGTG 
CAGTACTGGG GGAAGCAGTA TGATGTTCAC AGCCTCTATG GGTACAGCAT GGCTATAGCC 
ACAGAGAAAG CTGTAGAAAA AGTTTTTCCT AGTAAGAGAA GCTTTATTCT CACCCGGTCA 
ACTTTTGCTG GATCTGGGCA CTATGCTGCA CACTGGTTAG GAGACAATAC TGCTTCATGG 
GAACAAATGG AATGGTCTAT TGCAGGAATG CTGGAGTTCA GTTTGTTTGG AATGCCTTTG 
GTTGGAGCAG ACATCTGTGG ATTTGTGGTT AACACCACAG AAGAGCTTTG CAGAAGGTGG 
ATGCAACTTG GGGCATTTTA TCCATTTTCC AGGAACCATA ATGCTGATGG ATATGAGCAT 
CAGGATCCAG CATTTTTTGG CCAGAATTCC CTTTTGGTTA ATTCATCAAG GCACTATTTG 
AATATTCGCT ATACCTTATT ACCTTTCCTC TATACTCTGT TTTACAAAGC CCATGTGTTT 
GGAGAAACGG TAGCAAGGCC CGTTCTTCAT GAGTTTTATG ATGACAGAGA AAGCTGGATT 
GAGGACACTC AGTTCTTATG GGGCCCTGCG TTACTTATTA CTCCCGTCTT AAAAGAGGGA 
ACAGATACAG TGAGTGCATA CATCCCTAAT GCTACTTGGT ATGATTATGA AACTGGTGCA 
AAAAGGCCAT GGAAAAAACA GCGTGTTAAT ATGTATCTTC CGGGCGACAA AATAGGATTA 
CATCTTAGAG GAGGTTATAT TATCCCCATT CAGCAACCTG CTGTAACTAC AACTGCAAGC 
CGAAAGAACC CTCTAGGACT TATAGTTGCA TTGGATGACA ATAACATAGC AAAAGGGGAC 
TTTTTCTGGG ATGATGGAGA AACTAAGAAT ACCGTACAAA ATGGCAATTA CATTTTATAT 
ACTTTTTCAG TTTCCAATAA CAAATTAGAT ATTATATGCA CACATTCATC ATACCAGGAA 
GGAACCACTT TAGCATTTGA GACTATAAAA ATCCTTGGGT TGATAGATTC TGTTACACAA 
GTTACAGTGG TGGAAAATAA TCAGCCAATG AAGAATCACT ACAATTTTAC TTATACTGCT 
TCCAACCAGA GTCTCCTAAT TTACAATCTC AAGTTGAACC TTGGAGGAAA CTTTACTGTT 
CAGTGGAATC AAAATTTCTC AGAAAATGAA AAGTTTAATT GTTACCCAGA TGCAGACACT 
GCAACTAAAG AAAAATGTGA AGCACGTGGC TGTTTATGGG AAACGTCTTT TAGCTCCAAA 
GCACCAGAGT GTTACTTTCC TAGACAATCC AACCCATATC TAGTCCGTTC AACTCAGTAT 
TCATCAATGG GTATAACAAC TGACCTGCAA CTTAATCCTA CAAGTGCTCG AATAAAGCTA 
CCTTCTGAAC CCATCTCAAC TCTTCGTGTG GAAGTGAAAT ATCACAAAAA TGATATGCTG 
CAGTTTAAGA TTTATGATCC CCAAACAAAG AGATATGAAG TTCCAATACC TTTAAACATT 
CCAGACGTGC CAACAAGTAC CTATGAAAAT AGGCTTTATG ATGTTGAAAT CAGAGAAAAT 
CCTTTTGGCA TCCAGGTTCG AAGAAGAAGT ACAGGAAGAG TTATTTGGGA TTCTCAGCTG 
CCAGGATTTG CTTTTAATGA CCAGTTTATT CAAATATCTA CTCGCCTGCC ATCAGAATAT 
ATATATGGTT TTGGGGAAGT GGAACATACA GCATTTAAGA GAGACCTGAA CTGGAATACC 
TGGGGAATGT TCACAAGAGA CCAACCCCCT GGTTATAAAC TTAATTCCTA TGGATTTCAT 
CCCTATTACA TGGCTCTGGA AGACGAAGGC TATGCTCATG GAGTTTTATT ACTTAACAGC 
AATGCAATGG ATGTTACATT TCAGCCAACT CCTGCTCTAA CTTACCGTGT GATTGGAGGA 
ATCTTGGACT TTTATATGTT TTTGGGCCCA ACTCCAGAAG TTGCAACAAA GCAATACCAT 
GAAGTAATTG GCCGACCAGT CATGCCACCT TACTGGGCTT TGGGATTCCA AATATGTCGT 
TATGGGTACA GAAATACTTC ACAAGTTCAG CAAGTATATG ATGAGATGGT GGCTGCTCAG 
ATCCCCTATG ATGTTCAGTA TACAGACATT GATTACATGG AAAGGCAACT AGACTTTACA 
ATTGATGAAA ACTTCCGTGA CCTTCCTGCG TTTGTTGACA AAATAAGACA GGAAGGAATG 
AGATACATTA TTATCCTGGA TCCAGCAATT TCAGGAAATG AAACAAAGTA TTACTCCGCA 
TTTGAAAGAG GACAGGAGAA AGATGTTTTT GTCAAATGGC CTAACACCAA TGATATTTGT 
TGGGCGAAGG TTTGGCCAGA TTTGCCCAAT ATAACAATAG ATGAGAGTCT AACTGAAGAT 
GAAGCTGTGA ATGCTTCCAG AGCTCATGTA GCTTTTCCAG ACTTTTTCAG GAATTCCACA 
GCAGAGTGGT GGGCAACAGA AATTATAGAT TTCTACAATA ACCAGATGAA GTTTGATGGC 
TTGTGGATTG ATATGAATGA ACCATCAAGT TTTGTACATG GAACAGTTTC TAATCAATGC 
AGGAACACAG AGTTAAATTA TCCACCTTAT CTCCCAGAAC TCACAAAAAG AACTAGTGGA 
TTACATTTCA GAACTATGTG TATGGAAACT GAGCAGATTC TTAGTGATGG ATCATCTGTT 
TTGCATTACA ATGTTCACAA TCTATATGGA TGGTCACAAA TGAAACCTAG TTATGATGCA 
TTACAGAAGA CAACTGGCAA AAGAGGAATT GTTATTTCTC GCTCCACCTA TCCTTCTGGT 
GGACGATGGG GAGGACATTG GCTTGGAGAC AATTATGCAA AATGGGACAA TCTGGACAAA 
TCAATCATTG GTATGATGGA ATTTAGTCTC TTTGGAATCT CATATACTGG AGCAGATATC 
TGTGGTTTCT TCAACAATTC AGAATATGAG CTCTGTGCCC GCTGGATGCA ACTTGGAGCA 
TTTTATCCAT ACTCAAGAAA TCACAACATT GCAAATACTA GGAGACAAGA TCCTGCTTCC 
TGGAATTCAA CTTTTTCTGA AATGTCAAGG AATATTCTAA ATATTAGATA TACTTTATTG 
CCCTATTTCT ATACACAGAT GCATGAAATT CATGTTAATG GTGGCACTGT TATTCGACCC 
CTTTTGCATG AGTTCTTTAA CGACAGGATA ACCTGGGATA TATTCAAGCA GTTCTTGTGG 
GGTCCTGCAT TTATGGTCAC CCCTGTATTG GAGCCTCATG CTAACACTGT ACAAGGCTAC 
GTCCCTGATG CCCGGTGGTT TGATTATCAT ACGGGTCAAG ATATTGGTGT CAAAGGATTT 
GTTTTATTTA ATGCTCCTTT AAATACAATA AACCTACATG TCCGTGGTGG TCATATCCTA 
CCATGTCAAG AGCCTGCTCA AAACACATTT CACAGCCGAC AAAATTACAT GAAGCTCATT 
GTTGCTGCAG ATGTTAACCA GAGGGCACAG GGGTCTCTGT TTTGGGATGA CGGAGAGAGT 
ATAGATTCCT ATGAAAGAGG CTTCTATTTC TCGGCACAAT TTAATTTAAA CAATAATACC 
TTGACAAGCA CTATATTGCA AAATGGTTAC ATAAACAAAG ATGAAATGAG GCTTGGGTTC 
ATCATCATAT GGGGGAAAGG AAAGACTCCT GTTACTGGGG TTAATCTCAT ATATAATGGA 
AATACAGAAG CAGTTAAATT TATTCAAGAA GCAGACAAGG AGATATTAAA TATTGATCTG 
ACAGTGAATA ATGTTACTCT AGATGAACCA TTTCAAATCA TCTGGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005639937.1
CDS	252..5462
Translation	MRGCCWKPWNNSIIPWCFFVDNHGYNVGQLTATSTGLEATLNRISSPTLFGNDITRVLFTTQNQTANRFRFKITDPNNKRYEVPHQFVKEFTGTAASNTLYDVQVINNPFSIKVIRKSNGRILFDTSIGPLVYSDQYLQISTKLPSEYMYGIGEHIHKRFRHDLNWKTWPIFTRDQLPGDNNNNLYGHHTFFMCIEDETGKSFGVFLMNSNAMEIFIQPTPVVTYRVTGGILDFYIFLGDTPEQVVQQYQELIGRPAMPAYWSLGFQLSRWNYKSLDVVKEVVKRNRDAGIPFDTQVTDVDYMEAKKDFTYDKVAFQGLPEFVQDLHDHGQKYVIILDPAISIDKLANGAAYETYDRGNAKNVWVNDSDGTTAIIGEVWPGLTVFPDFTNPNCIDWWANECSIFYQEVKYDGLWIDMNEVSSFIQGSQKGCNNNKLNYPPFTPDILDKLLYSKTICMDAVQYWGKQYDVHSLYGYSMAIATEKAVEKVFPSKRSFILTRSTFAGSGHYAAHWLGDNTASWEQMEWSIAGMLEFSLFGMPLVGADICGFVVNTTEELCRRWMQLGAFYPFSRNHNADGYEHQDPAFFGQNSLLVNSSRHYLNIRYTLLPFLYTLFYKAHVFGETVARPVLHEFYDDRESWIEDTQFLWGPALLITPVLKEGTDTVSAYIPNATWYDYETGAKRPWKKQRVNMYLPGDKIGLHLRGGYIIPIQQPAVTTTASRKNPLGLIVALDDNNIAKGDFFWDDGETKNTVQNGNYILYTFSVSNNKLDIICTHSSYQEGTTLAFETIKILGLIDSVTQVTVVENNQPMKNHYNFTYTASNQSLLIYNLKLNLGGNFTVQWNQNFSENEKFNCYPDADTATKEKCEARGCLWETSFSSKAPECYFPRQSNPYLVRSTQYSSMGITTDLQLNPTSARIKLPSEPISTLRVEVKYHKNDMLQFKIYDPQTKRYEVPIPLNIPDVPTSTYENRLYDVEIRENPFGIQVRRRSTGRVIWDSQLPGFAFNDQFIQISTRLPSEYIYGFGEVEHTAFKRDLNWNTWGMFTRDQPPGYKLNSYGFHPYYMALEDEGYAHGVLLLNSNAMDVTFQPTPALTYRVIGGILDFYMFLGPTPEVATKQYHEVIGRPVMPPYWALGFQICRYGYRNTSQVQQVYDEMVAAQIPYDVQYTDIDYMERQLDFTIDENFRDLPAFVDKIRQEGMRYIIILDPAISGNETKYYSAFERGQEKDVFVKWPNTNDICWAKVWPDLPNITIDESLTEDEAVNASRAHVAFPDFFRNSTAEWWATEIIDFYNNQMKFDGLWIDMNEPSSFVHGTVSNQCRNTELNYPPYLPELTKRTSGLHFRTMCMETEQILSDGSSVLHYNVHNLYGWSQMKPSYDALQKTTGKRGIVISRSTYPSGGRWGGHWLGDNYAKWDNLDKSIIGMMEFSLFGISYTGADICGFFNNSEYELCARWMQLGAFYPYSRNHNIANTRRQDPASWNSTFSEMSRNILNIRYTLLPYFYTQMHEIHVNGGTVIRPLLHEFFNDRITWDIFKQFLWGPAFMVTPVLEPHANTVQGYVPDARWFDYHTGQDIGVKGFVLFNAPLNTINLHVRGGHILPCQEPAQNTFHSRQNYMKLIVAADVNQRAQGSLFWDDGESIDSYERGFYFSAQFNLNNNTLTSTILQNGYINKDEMRLGFIIIWGKGKTPVTGVNLIYNGNTEAVKFIQEADKEILNIDLTVNNVTLDEPFQIIWS

Target ORF information:

RefSeq Version	XM_005639880.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005639880.2

ORF Insert Sequence:

ATGCGAGGTT GCTGCTGGAA GCCATGGAAT AACTCTATTA TTCCTTGGTG CTTCTTCGTA 
GATAACCATG GTTATAATGT TGGACAACTG ACAGCAACAA GTACTGGACT TGAAGCCACA 
TTAAACAGGA TATCTTCACC TACATTGTTT GGAAATGACA TTACCCGTGT TCTCTTCACA 
ACTCAAAACC AAACAGCCAA TCGTTTCCGG TTTAAGATTA CTGATCCAAA TAACAAAAGA 
TATGAAGTCC CTCACCAGTT TGTAAAAGAA TTTACTGGAA CTGCAGCTTC TAATACATTG 
TATGATGTAC AGGTTATAAA TAATCCATTC AGCATCAAAG TCATTAGAAA AAGCAATGGG 
AGAATTTTAT TTGATACCAG CATTGGTCCC CTGGTTTACT CTGACCAGTA TTTACAGATC 
TCAACCAAAC TTCCTAGTGA ATATATGTAT GGTATTGGGG AACATATTCA TAAGAGATTT 
CGTCATGATT TAAACTGGAA AACATGGCCA ATTTTTACCC GGGATCAACT TCCTGGTGAT 
AATAATAATA ATTTATATGG CCATCATACA TTCTTCATGT GTATTGAAGA CGAGACTGGA 
AAATCATTTG GTGTTTTCTT AATGAACAGC AATGCAATGG AAATTTTCAT CCAGCCTACT 
CCAGTAGTAA CTTACAGAGT TACTGGTGGA ATTCTGGACT TTTACATCTT TCTAGGAGAT 
ACACCAGAAC AGGTAGTTCA ACAGTATCAA GAGCTCATTG GACGACCAGC AATGCCAGCC 
TATTGGAGTC TTGGATTCCA ACTGAGTCGC TGGAATTATA AGTCACTTGA TGTAGTGAAA 
GAAGTGGTAA AAAGAAATAG AGATGCTGGC ATACCATTTG ATACACAAGT CACTGATGTT 
GACTATATGG AAGCCAAGAA AGATTTTACT TATGACAAAG TTGCATTTCA GGGGCTCCCT 
GAATTTGTTC AAGATTTACA TGACCATGGA CAGAAATATG TCATCATCTT GGACCCTGCA 
ATTTCCATAG ATAAGCTTGC CAATGGAGCA GCATATGAAA CCTATGACAG GGGAAATGCA 
AAAAATGTGT GGGTAAATGA TTCAGATGGG ACTACAGCAA TTATTGGAGA GGTATGGCCA 
GGATTAACAG TATTCCCTGA CTTTACTAAT CCAAACTGCA TAGATTGGTG GGCAAATGAA 
TGCAGCATTT TCTATCAAGA AGTGAAATAT GATGGACTTT GGATTGACAT GAATGAAGTT 
TCCAGCTTTA TTCAAGGTTC ACAGAAAGGA TGCAATAACA ACAAACTGAA TTATCCACCT 
TTTACTCCTG ATATTCTTGA CAAACTCCTG TATTCCAAAA CTATTTGCAT GGATGCTGTG 
CAGTACTGGG GGAAGCAGTA TGATGTTCAC AGCCTCTATG GGTACAGCAT GGCTATAGCC 
ACAGAGAAAG CTGTAGAAAA AGTTTTTCCT AGTAAGAGAA GCTTTATTCT CACCCGGTCA 
ACTTTTGCTG GATCTGGGCA CTATGCTGCA CACTGGTTAG GAGACAATAC TGCTTCATGG 
GAACAAATGG AATGGTCTAT TGCAGGAATG CTGGAGTTCA GTTTGTTTGG AATGCCTTTG 
GTTGGAGCAG ACATCTGTGG ATTTGTGGTT AACACCACAG AAGAGCTTTG CAGAAGGTGG 
ATGCAACTTG GGGCATTTTA TCCATTTTCC AGGAACCATA ATGCTGATGG ATATGAGCAT 
CAGGATCCAG CATTTTTTGG CCAGAATTCC CTTTTGGTTA ATTCATCAAG GCACTATTTG 
AATATTCGCT ATACCTTATT ACCTTTCCTC TATACTCTGT TTTACAAAGC CCATGTGTTT 
GGAGAAACGG TAGCAAGGCC CGTTCTTCAT GAGTTTTATG ATGACAGAGA AAGCTGGATT 
GAGGACACTC AGTTCTTATG GGGCCCTGCG TTACTTATTA CTCCCGTCTT AAAAGAGGGA 
ACAGATACAG TGAGTGCATA CATCCCTAAT GCTACTTGGT ATGATTATGA AACTGGTGCA 
AAAAGGCCAT GGAAAAAACA GCGTGTTAAT ATGTATCTTC CGGGCGACAA AATAGGATTA 
CATCTTAGAG GAGGTTATAT TATCCCCATT CAGCAACCTG CTGTAACTAC AACTGCAAGC 
CGAAAGAACC CTCTAGGACT TATAGTTGCA TTGGATGACA ATAACATAGC AAAAGGGGAC 
TTTTTCTGGG ATGATGGAGA AACTAAGAAT ACCGTACAAA ATGGCAATTA CATTTTATAT 
ACTTTTTCAG TTTCCAATAA CAAATTAGAT ATTATATGCA CACATTCATC ATACCAGGAA 
GGAACCACTT TAGCATTTGA GACTATAAAA ATCCTTGGGT TGATAGATTC TGTTACACAA 
GTTACAGTGG TGGAAAATAA TCAGCCAATG AAGAATCACT ACAATTTTAC TTATACTGCT 
TCCAACCAGA GTCTCCTAAT TTACAATCTC AAGTTGAACC TTGGAGGAAA CTTTACTGTT 
CAGTGGAATC AAAATTTCTC AGAAAATGAA AAGTTTAATT GTTACCCAGA TGCAGACACT 
GCAACTAAAG AAAAATGTGA AGCACGTGGC TGTTTATGGG AAACGTCTTT TAGCTCCAAA 
GCACCAGAGT GTTACTTTCC TAGACAATCC AACCCATATC TAGTCCGTTC AACTCAGTAT 
TCATCAATGG GTATAACAAC TGACCTGCAA CTTAATCCTA CAAGTGCTCG AATAAAGCTA 
CCTTCTGAAC CCATCTCAAC TCTTCGTGTG GAAGTGAAAT ATCACAAAAA TGATATGCTG 
CAGTTTAAGA TTTATGATCC CCAAACAAAG AGATATGAAG TTCCAATACC TTTAAACATT 
CCAGACGTGC CAACAAGTAC CTATGAAAAT AGGCTTTATG ATGTTGAAAT CAGAGAAAAT 
CCTTTTGGCA TCCAGGTTCG AAGAAGAAGT ACAGGAAGAG TTATTTGGGA TTCTCAGCTG 
CCAGGATTTG CTTTTAATGA CCAGTTTATT CAAATATCTA CTCGCCTGCC ATCAGAATAT 
ATATATGGTT TTGGGGAAGT GGAACATACA GCATTTAAGA GAGACCTGAA CTGGAATACC 
TGGGGAATGT TCACAAGAGA CCAACCCCCT GGTTATAAAC TTAATTCCTA TGGATTTCAT 
CCCTATTACA TGGCTCTGGA AGACGAAGGC TATGCTCATG GAGTTTTATT ACTTAACAGC 
AATGCAATGG ATGTTACATT TCAGCCAACT CCTGCTCTAA CTTACCGTGT GATTGGAGGA 
ATCTTGGACT TTTATATGTT TTTGGGCCCA ACTCCAGAAG TTGCAACAAA GCAATACCAT 
GAAGTAATTG GCCGACCAGT CATGCCACCT TACTGGGCTT TGGGATTCCA AATATGTCGT 
TATGGGTACA GAAATACTTC ACAAGTTCAG CAAGTATATG ATGAGATGGT GGCTGCTCAG 
ATCCCCTATG ATGTTCAGTA TACAGACATT GATTACATGG AAAGGCAACT AGACTTTACA 
ATTGATGAAA ACTTCCGTGA CCTTCCTGCG TTTGTTGACA AAATAAGACA GGAAGGAATG 
AGATACATTA TTATCCTGGA TCCAGCAATT TCAGGAAATG AAACAAAGTA TTACTCCGCA 
TTTGAAAGAG GACAGGAGAA AGATGTTTTT GTCAAATGGC CTAACACCAA TGATATTTGT 
TGGGCGAAGG TTTGGCCAGA TTTGCCCAAT ATAACAATAG ATGAGAGTCT AACTGAAGAT 
GAAGCTGTGA ATGCTTCCAG AGCTCATGTA GCTTTTCCAG ACTTTTTCAG GAATTCCACA 
GCAGAGTGGT GGGCAACAGA AATTATAGAT TTCTACAATA ACCAGATGAA GTTTGATGGC 
TTGTGGATTG ATATGAATGA ACCATCAAGT TTTGTACATG GAACAGTTTC TAATCAATGC 
AGGAACACAG AGTTAAATTA TCCACCTTAT CTCCCAGAAC TCACAAAAAG AACTAGTGGA 
TTACATTTCA GAACTATGTG TATGGAAACT GAGCAGATTC TTAGTGATGG ATCATCTGTT 
TTGCATTACA ATGTTCACAA TCTATATGGA TGGTCACAAA TGAAACCTAG TTATGATGCA 
TTACAGAAGA CAACTGGCAA AAGAGGAATT GTTATTTCTC GCTCCACCTA TCCTTCTGGT 
GGACGATGGG GAGGACATTG GCTTGGAGAC AATTATGCAA AATGGGACAA TCTGGACAAA 
TCAATCATTG GTATGATGGA ATTTAGTCTC TTTGGAATCT CATATACTGG AGCAGATATC 
TGTGGTTTCT TCAACAATTC AGAATATGAG CTCTGTGCCC GCTGGATGCA ACTTGGAGCA 
TTTTATCCAT ACTCAAGAAA TCACAACATT GCAAATACTA GGAGACAAGA TCCTGCTTCC 
TGGAATTCAA CTTTTTCTGA AATGTCAAGG AATATTCTAA ATATTAGATA TACTTTATTG 
CCCTATTTCT ATACACAGAT GCATGAAATT CATGTTAATG GTGGCACTGT TATTCGACCC 
CTTTTGCATG AGTTCTTTAA CGACAGGATA ACCTGGGATA TATTCAAGCA GTTCTTGTGG 
GGTCCTGCAT TTATGGTCAC CCCTGTATTG GAGCCTCATG CTAACACTGT ACAAGGCTAC 
GTCCCTGATG CCCGGTGGTT TGATTATCAT ACGGGTCAAG ATATTGGTGT CAAAGGATTT 
GTTTTATTTA ATGCTCCTTT AAATACAATA AACCTACATG TCCGTGGTGG TCATATCCTA 
CCATGTCAAG AGCCTGCTCA AAACACATTT CACAGCCGAC AAAATTACAT GAAGCTCATT 
GTTGCTGCAG ATGTTAACCA GAGGGCACAG GGGTCTCTGT TTTGGGATGA CGGAGAGAGT 
ATAGATTCCT ATGAAAGAGG CTTCTATTTC TCGGCACAAT TTAATTTAAA CAATAATACC 
TTGACAAGCA CTATATTGCA AAATGGTTAC ATAAACAAAG ATGAAATGAG GCTTGGGTTC 
ATCATCATAT GGGGGAAAGG AAAGACTCCT GTTACTGGGG TTAATCTCAT ATATAATGGA 
AATACAGAAG CAGTTAAATT TATTCAAGAA GCAGACAAGG AGATATTAAA TATTGATCTG 
ACAGTGAATA ATGTTACTCT AGATGAACCA TTTCAAATCA TCTGGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SI cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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