CACNA1B cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1B
Entrez Gene ID	480660
Full Name	calcium voltage-gated channel subunit alpha1 B
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 B Names voltage-dependent N-type calcium channel subunit alpha-1B LOW QUALITY PROTEIN: voltage-dependent N-type calcium channel subunit alpha-1B
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014116864.2	XP_013972339.1	LOW QUALITY PROTEIN: voltage-dependent N-type calcium channel subunit alpha-1B
XM_014116864.1	XP_013972339.1	LOW QUALITY PROTEIN: voltage-dependent N-type calcium channel subunit alpha-1B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CACNA1B	XP_520396.4
Canis lupus familiaris (dog)	CACNA1B	XP_005625483.1
Rattus norvegicus (Norway rat)	Cacna1b	NP_671482.1
Danio rerio (zebrafish)	cacna1ba	NP_001108020.1
Caenorhabditis elegans (roundworm)	unc-2	NP_001123176.1
Gallus gallus (chicken)	CACNA1B	NP_989624.1
Drosophila melanogaster (fruit fly)	cac	NP_001245636.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002578	XP_312358.4
Homo sapiens (human)	CACNA1B	NP_000709.1
Bos taurus (cattle)	CACNA1B	NP_777057.1
Mus musculus (house mouse)	Cacna1b	NP_001035993.1
Danio rerio (zebrafish)	cacna1bb	XP_002665969.2

Gene Ontology

The following CACNA1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe38555	XM_014116864.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 B (CACNA1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
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OCe38555	XM_014116864.1	Canis lupus familiaris calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1B ( XM_014116864.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972339.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 B (CACNA1B), mRNA.

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CloneID	OCe38555
Clone ID Related Accession (Same CDS sequence)	XM_014116864.2 , XM_014116864.1
Accession Version	XM_014116864.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	LOW QUALITY PROTEIN: voltage-dependent N-type calcium channel subunit alpha-1B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116864.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCAGC CGCTATGGGG GCGCCGACGG GGAGCGGTCT 
CGGGACGGCG GGGCCGGCGG GGCGGGCGGC CCGGGCCCCG GGGGGCTGCA GCCGGGCCAG 
CGGGTGCTCT ACAAGCAGTC GATCGCACAA AGGGCGCGGA CCATGGCGCT GTACAACCCC 
ATCCCGGTCA AGCAGAACTG CATCACGGTG AACCGCTTGT TCTTCGTAAT GGGCGAAGAC 
AAAGTCATTT GAAAATACGC CAAGCGCATC ACCGAGTGGC CTCCGTTTGA GTACATGATC 
CTGGCCACTA TCATCGCCAA CTGTATCGTG CTGGCACTGG AGCAGCACCT TCCCGACGGG 
GACAAGACGC CCATGTCTGA GCGGCTGGAC GACACAGAGC CCTATTTTAT TGGCATCTTC 
TGCTTTGAGG CGGGGATCAA GATCATAGCT CTGGGCTTTG TCTTCCACAA GGGCTCCTAC 
CTGCGGAACG GCTGGAACGT CATGGACTTT GTGGTGGTTC TCACAGGGAT CCTGGCCACG 
GCTGGCACTG ACTTTGACCT GCGGACCCTG CGGGCTGTGC GCGTGCTCAG GCCCCTGAAG 
CTGGTGTCTG GGATTCCAAG TTTGCAGGTG GTGCTCAAGT CTATCATGAA GGCCATGGTT 
CCACTCCTGC AGATCGGGCT GCTTCTCTTC TTCGCCATCC TCATGTTCGC GATCATTGGC 
CTTGAGTTCT ACATGGGCAA ATTCCACAAG GCCTGTTTCT CCAACAGCAC AGATCCAGAT 
CCAGTGGGGG ACTTCCCCTG TGGCAAGGAG GCCCCGGCCC GGCTGTGTGA GGGTGACACT 
GAATGTCGGG AGTACTGGCC AGGGCCCAAC TTTGGCATCA CCAACTTTGA CAATATCCTG 
TTTGCCATCT TGACAGTATT CCAGTGCATC ACCATGGAGG GCTGGACTGA CATCCTCTAC 
AATACAAATG ATGCAGCTGG CAACACTTGG AACTGGCTCT ACTTCATCCC TCTCATCATC 
ATTGGCTCCT TCTTCATGCT CAACCTGGTG CTGGGTGTGC TCTCAGGAGA GTTTGCCAAG 
GAGCGTGAGA GGGTGGAGAA CCGCCGGGCA TTCCTGAAGC TCCGCCGCCA GCAGCAGATC 
GAGCGGGAGC TCAACGGGTA CCTGGAGTGG ATCTTCAAAG CCGAGGAAGT CATGCTGGCC 
GAGGAGGACA AGAATGCGGA GGAGAAGTCC CCCCTGGATG TGTTGAAGAG AGCAGCCACG 
AAGAAGAGCC GGAATGACCT GATCCATGCC GAGGAGGGAG AGGACCGGTT TCCTGACCTC 
TGTGCTGTTG GATCCCCCTT TGCCCGTGCC AGCCTCAAGA GCGGGAAGAC GGAGAGCTCG 
TCGTACTTCC GTAGGAAGGA GAAGATGTTC CGGTTCTTCA TCCGACGCAT GGTGAAGGCG 
CAGAGCTTCT ACTGGGTGGT GCTGTGTGTG GTGGCCCTGA ATACTCTGTG CGTGGCCATG 
GTGCACTACA ACCAGCCACA GCGGCTCACC ACGGCGCTGT ACTTTGCAGA GTTTGTTTTC 
CTGGGTCTCT TCCTCACAGA GATGTCATTG AAGATGTATG GCCTGGGGCC CAGAAGCTAC 
TTCCGATCTT CCTTCAACTG CTTTGACTTT GGGGTCATTG TGGGGAGCAT CTTTGAAGTG 
GTCTGGGCAG CCATCAAGCC AGGAACATCC TTTGGGATCA GTGTGCTGCG GGCACTGCGG 
TTGCTGAGGA TCTTCAAAGT CACAAAGTAC TGGAGCTCCC TGCGCAACCT GGTGGTATCT 
CTGCTAAACT CCATGAAGTC CATCATCAGC CTCCTCTTCC TGCTGTTCCT GTTCATCGTG 
GTCTTTGCTC TACTAGGAAT GCAGCTATTC GGGGGACAGT TTAACTTTAG AGATGAGACC 
CCGACAACCA ACTTTGACAC TTTCCCCGCG GCCATTCTCA CTGTCTTCCA GATCCTAACA 
GGAGAGGACT GGAATGCGGT GATGTATCAC GGGATCGAGT CACAAGGCGG AGTCAGCAAA 
GGCATGTTCT CCTCCTTTTA CTTCATCGTC CTGACGCTGT TTGGAAATTA CACGCTACTG 
AATGTCTTCC TGGCCATCGC CGTGGACAAC CTTGCCAATG CTCAGGAGCT GACCAAGGAC 
GAAGAGGAGA TGGAAGAAGC AGCCAATCAG AAGCTTGCTC TGCAAAAGGC CAAAGAAGTG 
GCTGAAGTCA GCCCCATGTC TGCTGCAAAC ATCTCCATCG CTGCTGCGCC GCAGAACTCG 
GCCAAGGCGC GCTCGGTGTG GGAGCAGCGG GCCAGCCAGC TGCGGCTGCA GAACCTGCGG 
GCCAGCTGCG AGGCGCTGTA CAGCGAGATG GACCCGGACG AGCGGCTGCG CTACGCCACC 
ACGCGCCACC TGCGCCCCGA CATGAAGACG CACCTGGACC GGCCGCTCGT GGTGGAGCTG 
GGCCGCGACG GGCCGCGGGT TCCCGCCGGG GGCAAGGCCC GGCCCGAGGG CGCGGAGGCC 
GCCGAGGACC TCTCAGAACT CATGGCACTG GAGGCCACAG CTCAGGATCA GCCCAGTCCA 
GGCACCGTCT TTATGGAGCC ACACGGTGAC CTAGAAGCCA GTGGGACGAT GGGTGTGGGC 
CCTGTACATG CATTGCCCAG CACTTGTCTA CAGAAGGTGG AGGAACAGCC AGAGGATGCA 
GATAATCAGC GGAATGTCAC ACGGATGGGC AGCCAACCCT CAGACCTGAG TGCCACTGTG 
CATATCCCAG TGACGCTGAC CGGTCCTCCT GGGGACACTG CGGCTGTTCC CAGTGGTAAT 
GTGGACCTGG AAAGTCAAGC CGAGGGGAAG AAGGAGGTGG AAGCTGACGA TGTGATGAGG 
AGCGGGCCCC GACCCATCGT CCCATACAGC TCTATGTTCT GTTTAAGCCC CACCAACCTG 
CTCCGCCGCT TCTGTCATTA CATCGTGACC ATGCGGTACT TCGAGATGGT CATTCTTGTG 
GTCATCGCCC TGAGCAGCAT TGCCCTGGCT GCCGAGGACC CAGTGCGGAC AGACTCGCCT 
AGGAACAACG CCCTGAAGTA TATGGACTAC ATTTTCACGG GCGTCTTCAC CTTTGAGATG 
GTGATAAAGA TGATTGATTT GGGTCTGCTG CTGCATCCTG GGGCCTACTT CCGGGACCTG 
TGGAATATTC TGGACTTCAT CGTGGTCAGT GGGGCCCTGG TGGCGTTTGC CTTCTCAGGA 
TCCAAAGGGA AAGACATCAG CACCATCAAG TCCCTGAGAG TCTTGCGTGT CCTGCGGCCC 
CTCAAGACTA TAAAACGGCT GCCTAAGCTC AAGGCCGTGT TTGACTGTGT GGTGAACTCC 
CTGAAGAACG TCCTCAACAT CCTGGTCGTC TACATGCTCT TCATGTTCAT TTTTGCTGTC 
ATTGCGGTGC AGCTCTTCAA AGGGAAGTTT TTCTACTGCA CGGATGAGTC CAAGGGGCTG 
GAGAGGGACT GCCGGGGTCA ATATTTGGAT TATGAGAAGG AGGAGGTGGA AGCTCAACCA 
CGGCAGTGGA AGAAATATGA TTTTCACTAT GACAACGTGC TCTGGGCTCT GCTGACACTG 
TTCACAGTGT CCACGGGAGA AGGGTGGCCC ATGGTCCTAA AACACTCTGT GGACGCCACC 
TATGAGGAGC AGGGCCCCAG CCCCGGGTAT CGCATGGAGC TGTCCATCTT CTATGTGGTC 
TACTTCGTGG TTTTCCCCTT CTTCTTTGTC AATATCTTTG TGGCCTTGAT CATCATCACC 
TTCCAGGAGC AGGGGGACAA GGTGATGTCT GAATGCAGCC TGGAGAAGAA TGAGAGGGCT 
TGCATCGACT TTGCCATCAG TGCCAAGCCC CTGACGCGAT ACATGCCCCA GGACAAGCAG 
TCATTTCAGT ACAAGACATG GACGTTTGTG GTCTCACCTC CCTTTGAGTA CTTCATCATG 
GCTATGATCG CCCTCAACAC AGTGGTGCTA ATGATGAAGT TCTATGATGC ACCGTATGAA 
TATGAACTAA TGTTGAAATG CCTGAACATT GTGTTCACAT CCATGTTCTC TATGGAATGC 
GTGCTGAAGA TCATCGCCTT CGGGGTGCTG AACTATTTCA GAGATGCCTG GAATGTCTTT 
GACTTTGTCA CTGTGTTGGG AAGTATTACT GATATTTTAG TAACAGAGAT TGCGGAAACG 
AACAATTTCA TCAACCTCAG CTTCCTCCGC CTCTTCCGTG CTGCCCGTCT CATCAAGCTG 
CTCCGCCAAG GCTACACCAT CCGCATCCTG CTGTGGACCT TCGTCCAGTC CTTCAAGGCC 
CTGCCCTACG TGTGTCTGCT CATCGCCATG CTGTTCTTCA TCTACGCCAT CATTGGCATG 
CAGGTGTTTG GGAATATTGC CCTAGATGAT GACACCAGCA TCAACCGGCA TAACAACTTC 
CGGACGTTTT TACAAGCCTT GATGCTATTG TTCAGGAGTG CCACGGGGGA GGCCTGGCAC 
GAGATCATGC TGTCTTGTCT CAGCAACCAG GCCTGTGATG AGCACGCCAA TGCCAGTGAG 
TGTGGGAGCG ACTTTGCCTA CTTTTACTTC GTCTCCTTCA TCTTCCTTTG CTCCTTTCTG 
ATGTTGAACC TCTTTGTGGC CGTGATCATG GACAATTTTG AGTACCTTAC ACGGGACTCT 
TCCATCCTAG GGCCTCACCA CCTGGACGAG TTTATCCGGG TCTGGGCTGA GTACGACCCG 
GCTGCGTGTG GGCGCATCAG TTACAATGAC ATGTTTGAGA TGCTGAAACA CATGTCCCCA 
CCCCTGGGGC TGGGGAAGAA ATGCCCTGCT CGAGTTGCAT ACAAGCGCCT GGTTCGTATG 
AACATGCCCA TCTCCAATGA GGATATGACT GTCCACTTCA CGTCCACGCT GATGGCCCTC 
ATTCGGACCG CACTGGAGAT CAAGCTGGCT CCAGCTGGCA CGAAGCAGCA TCAGTGTGAT 
GCAGAGTTGA GGAAGGAAAT TTCCTCTGTG TGGGCCAATC TGCCCCAGAA GACCCTGGAT 
TTACTGGTGC CACCCCATAA ACCCGATGAG ATGACAGTGG GAAAAGTGTA TGCAGCTCTG 
ATGATATTTG ACTTCTATAA ACAGAACAAG ACCACCAGAG ACCAGATTCA CCAGGCCGCG 
GGAGGCCTGT CCCAGATGGG CCCTGTGTCG CTGTTCCACC CTCTGAAGGC CACACTGGAG 
CAGACACAGC CAGCTGTGCT CCGAGGAGCC CGGGTTTTCC TTCGGCAGAA GAGCTCTGCG 
TCCCTCAGCA ATGGTGGGGC CATACAAACC CAAGAGAGTG GCATCAAGGA GTCTGTTTCT 
TGGGGCACTC AGAGGACCCA GGAGGTACCT TGTGAGGCAA GGACACCCCT GGAGCGTGGC 
CACTCTACAG AGATCCCAGT GGTGCAGCCA GGAAAACCGG CTGTGGATGT CCAGATGCAG 
AGCATGGCCC TGAGAGGTCC TGATGGGGAG CCCCAACCTG GGCTGGAGAG CCAGGGACGA 
GCAGCTTCCA TGCCCCGCCT GGCTGCAGAG ACTCAGCCGG CCCCAGACGC CAGCCCCATG 
AAGCGCTCCA TCTCCACCCT GGCGCCCCAA CGCCCCCATG TGGCTCATCT CTGCAACGCA 
GCCCTGGACC GAGCACCAGC CAGCCAGGCT GTGCCCCACC ACCACCACCG CTGCCACCGC 
CGCAGGGACA GGAAGCAGAA GTCCCTGGAG AAGGGGGCCA GCCTGTCTGC AGACACAGAT 
GGTGCACCCA GCAGTGCTGC GGGGCCAGGG CCACCCCTGG GAGAGGGGCC CGCAGGCTGC 
CGGAGGGAGC GCAGGCAGGA GCGGGGTCGG TCCCAGGAGC GGAGGCAGCC CTCTTCCTCT 
TCCTCGGAGA AGCAGCGCTT CTACTCCTGT GACCGCTTTG GGGGCCGTGA GCCCTCACAG 
CCCAAACCAT CCCTCAGCAG CCACCCCACG TCACCGACAG CGGGGCAGGA GCCAGGACCC 
CCGAGACCGG GTGGTGGTTC AGTGAATGGG AGCCCCTTGT TGTCAACATC CGGTGCTAGC 
ACCCCTGGCC GCAGTGGGCG GAGGCAGCTC CCCCAGACTC CGCTGACTCC CCGTCCCAGC 
GTCACCTACA AGACGGCCAA CTCATCCCCT GTCCACTTTG TTGGGGCGCA GACCAGCCTC 
CCCACCTTCT CCCCTGGTCG GCTTAGTCGT GGCCTTTCTG AACACAACGC TCTGCTCCAG 
AGAGACCCCC TCAGCCAGCC CCTGGCCGCC AGCTCTAGAA TTGGCTCTGA CCCTTATTTG 
GGGCAGCGTC TGGACAGTGA GGACACTGCC TGCACTCAGC CTGAGGACAC ACTCACCTTT 
GAGGAGGCTG TGGCCACCAA CTCAGGCCGC TCCTCCCGGA CTTCCTACGT GTCCTCTCTG 
ACCTCCCAGT CCCACCCCCT CCGCCGCGTG CCCAACGGCT ACCACTGCAC TCTGGGACTC 
AGCTCGGGCG GCCGGGTTCG GCACAGCTAC CACCACCCTG ACCAAGACCA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972339.1
CDS	127..6666
Translation	MVRFGDELGSRYGGADGERSRDGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCITVNRLFFVMGEDKVI*KYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFSNSTDPDPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFPDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAAPQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPDERLRYATTRHLRPDMKTHLDRPLVVELGRDGPRVPAGGKARPEGAEAAEDLSELMALEATAQDQPSPGTVFMEPHGDLEASGTMGVGPVHALPSTCLQKVEEQPEDADNQRNVTRMGSQPSDLSATVHIPVTLTGPPGDTAAVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSPRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILVVYMLFMFIFAVIAVQLFKGKFFYCTDESKGLERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQDKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQIHQAAGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSASLSNGGAIQTQESGIKESVSWGTQRTQEVPCEARTPLERGHSTEIPVVQPGKPAVDVQMQSMALRGPDGEPQPGLESQGRAASMPRLAAETQPAPDASPMKRSISTLAPQRPHVAHLCNAALDRAPASQAVPHHHHRCHRRRDRKQKSLEKGASLSADTDGAPSSAAGPGPPLGEGPAGCRRERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPSQPKPSLSSHPTSPTAGQEPGPPRPGGGSVNGSPLLSTSGASTPGRSGRRQLPQTPLTPRPSVTYKTANSSPVHFVGAQTSLPTFSPGRLSRGLSEHNALLQRDPLSQPLAASSRIGSDPYLGQRLDSEDTACTQPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRVRHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_014116864.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 B (CACNA1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116864.2

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCAGC CGCTATGGGG GCGCCGACGG GGAGCGGTCT 
CGGGACGGCG GGGCCGGCGG GGCGGGCGGC CCGGGCCCCG GGGGGCTGCA GCCGGGCCAG 
CGGGTGCTCT ACAAGCAGTC GATCGCACAA AGGGCGCGGA CCATGGCGCT GTACAACCCC 
ATCCCGGTCA AGCAGAACTG CATCACGGTG AACCGCTTGT TCTTCGTAAT GGGCGAAGAC 
AAAGTCATTT GAAAATACGC CAAGCGCATC ACCGAGTGGC CTCCGTTTGA GTACATGATC 
CTGGCCACTA TCATCGCCAA CTGTATCGTG CTGGCACTGG AGCAGCACCT TCCCGACGGG 
GACAAGACGC CCATGTCTGA GCGGCTGGAC GACACAGAGC CCTATTTTAT TGGCATCTTC 
TGCTTTGAGG CGGGGATCAA GATCATAGCT CTGGGCTTTG TCTTCCACAA GGGCTCCTAC 
CTGCGGAACG GCTGGAACGT CATGGACTTT GTGGTGGTTC TCACAGGGAT CCTGGCCACG 
GCTGGCACTG ACTTTGACCT GCGGACCCTG CGGGCTGTGC GCGTGCTCAG GCCCCTGAAG 
CTGGTGTCTG GGATTCCAAG TTTGCAGGTG GTGCTCAAGT CTATCATGAA GGCCATGGTT 
CCACTCCTGC AGATCGGGCT GCTTCTCTTC TTCGCCATCC TCATGTTCGC GATCATTGGC 
CTTGAGTTCT ACATGGGCAA ATTCCACAAG GCCTGTTTCT CCAACAGCAC AGATCCAGAT 
CCAGTGGGGG ACTTCCCCTG TGGCAAGGAG GCCCCGGCCC GGCTGTGTGA GGGTGACACT 
GAATGTCGGG AGTACTGGCC AGGGCCCAAC TTTGGCATCA CCAACTTTGA CAATATCCTG 
TTTGCCATCT TGACAGTATT CCAGTGCATC ACCATGGAGG GCTGGACTGA CATCCTCTAC 
AATACAAATG ATGCAGCTGG CAACACTTGG AACTGGCTCT ACTTCATCCC TCTCATCATC 
ATTGGCTCCT TCTTCATGCT CAACCTGGTG CTGGGTGTGC TCTCAGGAGA GTTTGCCAAG 
GAGCGTGAGA GGGTGGAGAA CCGCCGGGCA TTCCTGAAGC TCCGCCGCCA GCAGCAGATC 
GAGCGGGAGC TCAACGGGTA CCTGGAGTGG ATCTTCAAAG CCGAGGAAGT CATGCTGGCC 
GAGGAGGACA AGAATGCGGA GGAGAAGTCC CCCCTGGATG TGTTGAAGAG AGCAGCCACG 
AAGAAGAGCC GGAATGACCT GATCCATGCC GAGGAGGGAG AGGACCGGTT TCCTGACCTC 
TGTGCTGTTG GATCCCCCTT TGCCCGTGCC AGCCTCAAGA GCGGGAAGAC GGAGAGCTCG 
TCGTACTTCC GTAGGAAGGA GAAGATGTTC CGGTTCTTCA TCCGACGCAT GGTGAAGGCG 
CAGAGCTTCT ACTGGGTGGT GCTGTGTGTG GTGGCCCTGA ATACTCTGTG CGTGGCCATG 
GTGCACTACA ACCAGCCACA GCGGCTCACC ACGGCGCTGT ACTTTGCAGA GTTTGTTTTC 
CTGGGTCTCT TCCTCACAGA GATGTCATTG AAGATGTATG GCCTGGGGCC CAGAAGCTAC 
TTCCGATCTT CCTTCAACTG CTTTGACTTT GGGGTCATTG TGGGGAGCAT CTTTGAAGTG 
GTCTGGGCAG CCATCAAGCC AGGAACATCC TTTGGGATCA GTGTGCTGCG GGCACTGCGG 
TTGCTGAGGA TCTTCAAAGT CACAAAGTAC TGGAGCTCCC TGCGCAACCT GGTGGTATCT 
CTGCTAAACT CCATGAAGTC CATCATCAGC CTCCTCTTCC TGCTGTTCCT GTTCATCGTG 
GTCTTTGCTC TACTAGGAAT GCAGCTATTC GGGGGACAGT TTAACTTTAG AGATGAGACC 
CCGACAACCA ACTTTGACAC TTTCCCCGCG GCCATTCTCA CTGTCTTCCA GATCCTAACA 
GGAGAGGACT GGAATGCGGT GATGTATCAC GGGATCGAGT CACAAGGCGG AGTCAGCAAA 
GGCATGTTCT CCTCCTTTTA CTTCATCGTC CTGACGCTGT TTGGAAATTA CACGCTACTG 
AATGTCTTCC TGGCCATCGC CGTGGACAAC CTTGCCAATG CTCAGGAGCT GACCAAGGAC 
GAAGAGGAGA TGGAAGAAGC AGCCAATCAG AAGCTTGCTC TGCAAAAGGC CAAAGAAGTG 
GCTGAAGTCA GCCCCATGTC TGCTGCAAAC ATCTCCATCG CTGCTGCGCC GCAGAACTCG 
GCCAAGGCGC GCTCGGTGTG GGAGCAGCGG GCCAGCCAGC TGCGGCTGCA GAACCTGCGG 
GCCAGCTGCG AGGCGCTGTA CAGCGAGATG GACCCGGACG AGCGGCTGCG CTACGCCACC 
ACGCGCCACC TGCGCCCCGA CATGAAGACG CACCTGGACC GGCCGCTCGT GGTGGAGCTG 
GGCCGCGACG GGCCGCGGGT TCCCGCCGGG GGCAAGGCCC GGCCCGAGGG CGCGGAGGCC 
GCCGAGGACC TCTCAGAACT CATGGCACTG GAGGCCACAG CTCAGGATCA GCCCAGTCCA 
GGCACCGTCT TTATGGAGCC ACACGGTGAC CTAGAAGCCA GTGGGACGAT GGGTGTGGGC 
CCTGTACATG CATTGCCCAG CACTTGTCTA CAGAAGGTGG AGGAACAGCC AGAGGATGCA 
GATAATCAGC GGAATGTCAC ACGGATGGGC AGCCAACCCT CAGACCTGAG TGCCACTGTG 
CATATCCCAG TGACGCTGAC CGGTCCTCCT GGGGACACTG CGGCTGTTCC CAGTGGTAAT 
GTGGACCTGG AAAGTCAAGC CGAGGGGAAG AAGGAGGTGG AAGCTGACGA TGTGATGAGG 
AGCGGGCCCC GACCCATCGT CCCATACAGC TCTATGTTCT GTTTAAGCCC CACCAACCTG 
CTCCGCCGCT TCTGTCATTA CATCGTGACC ATGCGGTACT TCGAGATGGT CATTCTTGTG 
GTCATCGCCC TGAGCAGCAT TGCCCTGGCT GCCGAGGACC CAGTGCGGAC AGACTCGCCT 
AGGAACAACG CCCTGAAGTA TATGGACTAC ATTTTCACGG GCGTCTTCAC CTTTGAGATG 
GTGATAAAGA TGATTGATTT GGGTCTGCTG CTGCATCCTG GGGCCTACTT CCGGGACCTG 
TGGAATATTC TGGACTTCAT CGTGGTCAGT GGGGCCCTGG TGGCGTTTGC CTTCTCAGGA 
TCCAAAGGGA AAGACATCAG CACCATCAAG TCCCTGAGAG TCTTGCGTGT CCTGCGGCCC 
CTCAAGACTA TAAAACGGCT GCCTAAGCTC AAGGCCGTGT TTGACTGTGT GGTGAACTCC 
CTGAAGAACG TCCTCAACAT CCTGGTCGTC TACATGCTCT TCATGTTCAT TTTTGCTGTC 
ATTGCGGTGC AGCTCTTCAA AGGGAAGTTT TTCTACTGCA CGGATGAGTC CAAGGGGCTG 
GAGAGGGACT GCCGGGGTCA ATATTTGGAT TATGAGAAGG AGGAGGTGGA AGCTCAACCA 
CGGCAGTGGA AGAAATATGA TTTTCACTAT GACAACGTGC TCTGGGCTCT GCTGACACTG 
TTCACAGTGT CCACGGGAGA AGGGTGGCCC ATGGTCCTAA AACACTCTGT GGACGCCACC 
TATGAGGAGC AGGGCCCCAG CCCCGGGTAT CGCATGGAGC TGTCCATCTT CTATGTGGTC 
TACTTCGTGG TTTTCCCCTT CTTCTTTGTC AATATCTTTG TGGCCTTGAT CATCATCACC 
TTCCAGGAGC AGGGGGACAA GGTGATGTCT GAATGCAGCC TGGAGAAGAA TGAGAGGGCT 
TGCATCGACT TTGCCATCAG TGCCAAGCCC CTGACGCGAT ACATGCCCCA GGACAAGCAG 
TCATTTCAGT ACAAGACATG GACGTTTGTG GTCTCACCTC CCTTTGAGTA CTTCATCATG 
GCTATGATCG CCCTCAACAC AGTGGTGCTA ATGATGAAGT TCTATGATGC ACCGTATGAA 
TATGAACTAA TGTTGAAATG CCTGAACATT GTGTTCACAT CCATGTTCTC TATGGAATGC 
GTGCTGAAGA TCATCGCCTT CGGGGTGCTG AACTATTTCA GAGATGCCTG GAATGTCTTT 
GACTTTGTCA CTGTGTTGGG AAGTATTACT GATATTTTAG TAACAGAGAT TGCGGAAACG 
AACAATTTCA TCAACCTCAG CTTCCTCCGC CTCTTCCGTG CTGCCCGTCT CATCAAGCTG 
CTCCGCCAAG GCTACACCAT CCGCATCCTG CTGTGGACCT TCGTCCAGTC CTTCAAGGCC 
CTGCCCTACG TGTGTCTGCT CATCGCCATG CTGTTCTTCA TCTACGCCAT CATTGGCATG 
CAGGTGTTTG GGAATATTGC CCTAGATGAT GACACCAGCA TCAACCGGCA TAACAACTTC 
CGGACGTTTT TACAAGCCTT GATGCTATTG TTCAGGAGTG CCACGGGGGA GGCCTGGCAC 
GAGATCATGC TGTCTTGTCT CAGCAACCAG GCCTGTGATG AGCACGCCAA TGCCAGTGAG 
TGTGGGAGCG ACTTTGCCTA CTTTTACTTC GTCTCCTTCA TCTTCCTTTG CTCCTTTCTG 
ATGTTGAACC TCTTTGTGGC CGTGATCATG GACAATTTTG AGTACCTTAC ACGGGACTCT 
TCCATCCTAG GGCCTCACCA CCTGGACGAG TTTATCCGGG TCTGGGCTGA GTACGACCCG 
GCTGCGTGTG GGCGCATCAG TTACAATGAC ATGTTTGAGA TGCTGAAACA CATGTCCCCA 
CCCCTGGGGC TGGGGAAGAA ATGCCCTGCT CGAGTTGCAT ACAAGCGCCT GGTTCGTATG 
AACATGCCCA TCTCCAATGA GGATATGACT GTCCACTTCA CGTCCACGCT GATGGCCCTC 
ATTCGGACCG CACTGGAGAT CAAGCTGGCT CCAGCTGGCA CGAAGCAGCA TCAGTGTGAT 
GCAGAGTTGA GGAAGGAAAT TTCCTCTGTG TGGGCCAATC TGCCCCAGAA GACCCTGGAT 
TTACTGGTGC CACCCCATAA ACCCGATGAG ATGACAGTGG GAAAAGTGTA TGCAGCTCTG 
ATGATATTTG ACTTCTATAA ACAGAACAAG ACCACCAGAG ACCAGATTCA CCAGGCCGCG 
GGAGGCCTGT CCCAGATGGG CCCTGTGTCG CTGTTCCACC CTCTGAAGGC CACACTGGAG 
CAGACACAGC CAGCTGTGCT CCGAGGAGCC CGGGTTTTCC TTCGGCAGAA GAGCTCTGCG 
TCCCTCAGCA ATGGTGGGGC CATACAAACC CAAGAGAGTG GCATCAAGGA GTCTGTTTCT 
TGGGGCACTC AGAGGACCCA GGAGGTACCT TGTGAGGCAA GGACACCCCT GGAGCGTGGC 
CACTCTACAG AGATCCCAGT GGTGCAGCCA GGAAAACCGG CTGTGGATGT CCAGATGCAG 
AGCATGGCCC TGAGAGGTCC TGATGGGGAG CCCCAACCTG GGCTGGAGAG CCAGGGACGA 
GCAGCTTCCA TGCCCCGCCT GGCTGCAGAG ACTCAGCCGG CCCCAGACGC CAGCCCCATG 
AAGCGCTCCA TCTCCACCCT GGCGCCCCAA CGCCCCCATG TGGCTCATCT CTGCAACGCA 
GCCCTGGACC GAGCACCAGC CAGCCAGGCT GTGCCCCACC ACCACCACCG CTGCCACCGC 
CGCAGGGACA GGAAGCAGAA GTCCCTGGAG AAGGGGGCCA GCCTGTCTGC AGACACAGAT 
GGTGCACCCA GCAGTGCTGC GGGGCCAGGG CCACCCCTGG GAGAGGGGCC CGCAGGCTGC 
CGGAGGGAGC GCAGGCAGGA GCGGGGTCGG TCCCAGGAGC GGAGGCAGCC CTCTTCCTCT 
TCCTCGGAGA AGCAGCGCTT CTACTCCTGT GACCGCTTTG GGGGCCGTGA GCCCTCACAG 
CCCAAACCAT CCCTCAGCAG CCACCCCACG TCACCGACAG CGGGGCAGGA GCCAGGACCC 
CCGAGACCGG GTGGTGGTTC AGTGAATGGG AGCCCCTTGT TGTCAACATC CGGTGCTAGC 
ACCCCTGGCC GCAGTGGGCG GAGGCAGCTC CCCCAGACTC CGCTGACTCC CCGTCCCAGC 
GTCACCTACA AGACGGCCAA CTCATCCCCT GTCCACTTTG TTGGGGCGCA GACCAGCCTC 
CCCACCTTCT CCCCTGGTCG GCTTAGTCGT GGCCTTTCTG AACACAACGC TCTGCTCCAG 
AGAGACCCCC TCAGCCAGCC CCTGGCCGCC AGCTCTAGAA TTGGCTCTGA CCCTTATTTG 
GGGCAGCGTC TGGACAGTGA GGACACTGCC TGCACTCAGC CTGAGGACAC ACTCACCTTT 
GAGGAGGCTG TGGCCACCAA CTCAGGCCGC TCCTCCCGGA CTTCCTACGT GTCCTCTCTG 
ACCTCCCAGT CCCACCCCCT CCGCCGCGTG CCCAACGGCT ACCACTGCAC TCTGGGACTC 
AGCTCGGGCG GCCGGGTTCG GCACAGCTAC CACCACCCTG ACCAAGACCA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_014116864.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972339.1 Canis lupus familiaris calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), mRNA.

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CloneID	OCe38555
Clone ID Related Accession (Same CDS sequence)	XM_014116864.2 , XM_014116864.1
Accession Version	XM_014116864.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	LOW QUALITY PROTEIN: voltage-dependent N-type calcium channel subunit alpha-1B
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCAGC CGCTATGGGG GCGCCGACGG GGAGCGGTCT 
CGGGACGGCG GGGCCGGCGG GGCGGGCGGC CCGGGCCCCG GGGGGCTGCA GCCGGGCCAG 
CGGGTGCTCT ACAAGCAGTC GATCGCACAA AGGGCGCGGA CCATGGCGCT GTACAACCCC 
ATCCCGGTCA AGCAGAACTG CATCACGGTG AACCGCTTGT TCTTCGTAAT GGGCGAAGAC 
AAAGTCATTT GAAAATACGC CAAGCGCATC ACCGAGTGGC CTCCGTTTGA GTACATGATC 
CTGGCCACTA TCATCGCCAA CTGTATCGTG CTGGCACTGG AGCAGCACCT TCCCGACGGG 
GACAAGACGC CCATGTCTGA GCGGCTGGAC GACACAGAGC CCTATTTTAT TGGCATCTTC 
TGCTTTGAGG CGGGGATCAA GATCATAGCT CTGGGCTTTG TCTTCCACAA GGGCTCCTAC 
CTGCGGAACG GCTGGAACGT CATGGACTTT GTGGTGGTTC TCACAGGGAT CCTGGCCACG 
GCTGGCACTG ACTTTGACCT GCGGACCCTG CGGGCTGTGC GCGTGCTCAG GCCCCTGAAG 
CTGGTGTCTG GGATTCCAAG TTTGCAGGTG GTGCTCAAGT CTATCATGAA GGCCATGGTT 
CCACTCCTGC AGATCGGGCT GCTTCTCTTC TTCGCCATCC TCATGTTCGC GATCATTGGC 
CTTGAGTTCT ACATGGGCAA ATTCCACAAG GCCTGTTTCT CCAACAGCAC AGATCCAGAT 
CCAGTGGGGG ACTTCCCCTG TGGCAAGGAG GCCCCGGCCC GGCTGTGTGA GGGTGACACT 
GAATGTCGGG AGTACTGGCC AGGGCCCAAC TTTGGCATCA CCAACTTTGA CAATATCCTG 
TTTGCCATCT TGACAGTATT CCAGTGCATC ACCATGGAGG GCTGGACTGA CATCCTCTAC 
AATACAAATG ATGCAGCTGG CAACACTTGG AACTGGCTCT ACTTCATCCC TCTCATCATC 
ATTGGCTCCT TCTTCATGCT CAACCTGGTG CTGGGTGTGC TCTCAGGAGA GTTTGCCAAG 
GAGCGTGAGA GGGTGGAGAA CCGCCGGGCA TTCCTGAAGC TCCGCCGCCA GCAGCAGATC 
GAGCGGGAGC TCAACGGGTA CCTGGAGTGG ATCTTCAAAG CCGAGGAAGT CATGCTGGCC 
GAGGAGGACA AGAATGCGGA GGAGAAGTCC CCCCTGGATG TGTTGAAGAG AGCAGCCACG 
AAGAAGAGCC GGAATGACCT GATCCATGCC GAGGAGGGAG AGGACCGGTT TCCTGACCTC 
TGTGCTGTTG GATCCCCCTT TGCCCGTGCC AGCCTCAAGA GCGGGAAGAC GGAGAGCTCG 
TCGTACTTCC GTAGGAAGGA GAAGATGTTC CGGTTCTTCA TCCGACGCAT GGTGAAGGCG 
CAGAGCTTCT ACTGGGTGGT GCTGTGTGTG GTGGCCCTGA ATACTCTGTG CGTGGCCATG 
GTGCACTACA ACCAGCCACA GCGGCTCACC ACGGCGCTGT ACTTTGCAGA GTTTGTTTTC 
CTGGGTCTCT TCCTCACAGA GATGTCATTG AAGATGTATG GCCTGGGGCC CAGAAGCTAC 
TTCCGATCTT CCTTCAACTG CTTTGACTTT GGGGTCATTG TGGGGAGCAT CTTTGAAGTG 
GTCTGGGCAG CCATCAAGCC AGGAACATCC TTTGGGATCA GTGTGCTGCG GGCACTGCGG 
TTGCTGAGGA TCTTCAAAGT CACAAAGTAC TGGAGCTCCC TGCGCAACCT GGTGGTATCT 
CTGCTAAACT CCATGAAGTC CATCATCAGC CTCCTCTTCC TGCTGTTCCT GTTCATCGTG 
GTCTTTGCTC TACTAGGAAT GCAGCTATTC GGGGGACAGT TTAACTTTAG AGATGAGACC 
CCGACAACCA ACTTTGACAC TTTCCCCGCG GCCATTCTCA CTGTCTTCCA GATCCTAACA 
GGAGAGGACT GGAATGCGGT GATGTATCAC GGGATCGAGT CACAAGGCGG AGTCAGCAAA 
GGCATGTTCT CCTCCTTTTA CTTCATCGTC CTGACGCTGT TTGGAAATTA CACGCTACTG 
AATGTCTTCC TGGCCATCGC CGTGGACAAC CTTGCCAATG CTCAGGAGCT GACCAAGGAC 
GAAGAGGAGA TGGAAGAAGC AGCCAATCAG AAGCTTGCTC TGCAAAAGGC CAAAGAAGTG 
GCTGAAGTCA GCCCCATGTC TGCTGCAAAC ATCTCCATCG CTGCTGCGCC GCAGAACTCG 
GCCAAGGCGC GCTCGGTGTG GGAGCAGCGG GCCAGCCAGC TGCGGCTGCA GAACCTGCGG 
GCCAGCTGCG AGGCGCTGTA CAGCGAGATG GACCCGGACG AGCGGCTGCG CTACGCCACC 
ACGCGCCACC TGCGCCCCGA CATGAAGACG CACCTGGACC GGCCGCTCGT GGTGGAGCTG 
GGCCGCGACG GGCCGCGGGT TCCCGCCGGG GGCAAGGCCC GGCCCGAGGG CGCGGAGGCC 
GCCGAGGACC TCTCAGAACT CATGGCACTG GAGGCCACAG CTCAGGATCA GCCCAGTCCA 
GGCACCGTCT TTATGGAGCC ACACGGTGAC CTAGAAGCCA GTGGGACGAT GGGTGTGGGC 
CCTGTACATG CATTGCCCAG CACTTGTCTA CAGAAGGTGG AGGAACAGCC AGAGGATGCA 
GATAATCAGC GGAATGTCAC ACGGATGGGC AGCCAACCCT CAGACCTGAG TGCCACTGTG 
CATATCCCAG TGACGCTGAC CGGTCCTCCT GGGGACACTG CGGCTGTTCC CAGTGGTAAT 
GTGGACCTGG AAAGTCAAGC CGAGGGGAAG AAGGAGGTGG AAGCTGACGA TGTGATGAGG 
AGCGGGCCCC GACCCATCGT CCCATACAGC TCTATGTTCT GTTTAAGCCC CACCAACCTG 
CTCCGCCGCT TCTGTCATTA CATCGTGACC ATGCGGTACT TCGAGATGGT CATTCTTGTG 
GTCATCGCCC TGAGCAGCAT TGCCCTGGCT GCCGAGGACC CAGTGCGGAC AGACTCGCCT 
AGGAACAACG CCCTGAAGTA TATGGACTAC ATTTTCACGG GCGTCTTCAC CTTTGAGATG 
GTGATAAAGA TGATTGATTT GGGTCTGCTG CTGCATCCTG GGGCCTACTT CCGGGACCTG 
TGGAATATTC TGGACTTCAT CGTGGTCAGT GGGGCCCTGG TGGCGTTTGC CTTCTCAGGA 
TCCAAAGGGA AAGACATCAG CACCATCAAG TCCCTGAGAG TCTTGCGTGT CCTGCGGCCC 
CTCAAGACTA TAAAACGGCT GCCTAAGCTC AAGGCCGTGT TTGACTGTGT GGTGAACTCC 
CTGAAGAACG TCCTCAACAT CCTGGTCGTC TACATGCTCT TCATGTTCAT TTTTGCTGTC 
ATTGCGGTGC AGCTCTTCAA AGGGAAGTTT TTCTACTGCA CGGATGAGTC CAAGGGGCTG 
GAGAGGGACT GCCGGGGTCA ATATTTGGAT TATGAGAAGG AGGAGGTGGA AGCTCAACCA 
CGGCAGTGGA AGAAATATGA TTTTCACTAT GACAACGTGC TCTGGGCTCT GCTGACACTG 
TTCACAGTGT CCACGGGAGA AGGGTGGCCC ATGGTCCTAA AACACTCTGT GGACGCCACC 
TATGAGGAGC AGGGCCCCAG CCCCGGGTAT CGCATGGAGC TGTCCATCTT CTATGTGGTC 
TACTTCGTGG TTTTCCCCTT CTTCTTTGTC AATATCTTTG TGGCCTTGAT CATCATCACC 
TTCCAGGAGC AGGGGGACAA GGTGATGTCT GAATGCAGCC TGGAGAAGAA TGAGAGGGCT 
TGCATCGACT TTGCCATCAG TGCCAAGCCC CTGACGCGAT ACATGCCCCA GGACAAGCAG 
TCATTTCAGT ACAAGACATG GACGTTTGTG GTCTCACCTC CCTTTGAGTA CTTCATCATG 
GCTATGATCG CCCTCAACAC AGTGGTGCTA ATGATGAAGT TCTATGATGC ACCGTATGAA 
TATGAACTAA TGTTGAAATG CCTGAACATT GTGTTCACAT CCATGTTCTC TATGGAATGC 
GTGCTGAAGA TCATCGCCTT CGGGGTGCTG AACTATTTCA GAGATGCCTG GAATGTCTTT 
GACTTTGTCA CTGTGTTGGG AAGTATTACT GATATTTTAG TAACAGAGAT TGCGGAAACG 
AACAATTTCA TCAACCTCAG CTTCCTCCGC CTCTTCCGTG CTGCCCGTCT CATCAAGCTG 
CTCCGCCAAG GCTACACCAT CCGCATCCTG CTGTGGACCT TCGTCCAGTC CTTCAAGGCC 
CTGCCCTACG TGTGTCTGCT CATCGCCATG CTGTTCTTCA TCTACGCCAT CATTGGCATG 
CAGGTGTTTG GGAATATTGC CCTAGATGAT GACACCAGCA TCAACCGGCA TAACAACTTC 
CGGACGTTTT TACAAGCCTT GATGCTATTG TTCAGGAGTG CCACGGGGGA GGCCTGGCAC 
GAGATCATGC TGTCTTGTCT CAGCAACCAG GCCTGTGATG AGCACGCCAA TGCCAGTGAG 
TGTGGGAGCG ACTTTGCCTA CTTTTACTTC GTCTCCTTCA TCTTCCTTTG CTCCTTTCTG 
ATGTTGAACC TCTTTGTGGC CGTGATCATG GACAATTTTG AGTACCTTAC ACGGGACTCT 
TCCATCCTAG GGCCTCACCA CCTGGACGAG TTTATCCGGG TCTGGGCTGA GTACGACCCG 
GCTGCGTGTG GGCGCATCAG TTACAATGAC ATGTTTGAGA TGCTGAAACA CATGTCCCCA 
CCCCTGGGGC TGGGGAAGAA ATGCCCTGCT CGAGTTGCAT ACAAGCGCCT GGTTCGTATG 
AACATGCCCA TCTCCAATGA GGATATGACT GTCCACTTCA CGTCCACGCT GATGGCCCTC 
ATTCGGACCG CACTGGAGAT CAAGCTGGCT CCAGCTGGCA CGAAGCAGCA TCAGTGTGAT 
GCAGAGTTGA GGAAGGAAAT TTCCTCTGTG TGGGCCAATC TGCCCCAGAA GACCCTGGAT 
TTACTGGTGC CACCCCATAA ACCCGATGAG ATGACAGTGG GAAAAGTGTA TGCAGCTCTG 
ATGATATTTG ACTTCTATAA ACAGAACAAG ACCACCAGAG ACCAGATTCA CCAGGCCGCG 
GGAGGCCTGT CCCAGATGGG CCCTGTGTCG CTGTTCCACC CTCTGAAGGC CACACTGGAG 
CAGACACAGC CAGCTGTGCT CCGAGGAGCC CGGGTTTTCC TTCGGCAGAA GAGCTCTGCG 
TCCCTCAGCA ATGGTGGGGC CATACAAACC CAAGAGAGTG GCATCAAGGA GTCTGTTTCT 
TGGGGCACTC AGAGGACCCA GGAGGTACCT TGTGAGGCAA GGACACCCCT GGAGCGTGGC 
CACTCTACAG AGATCCCAGT GGTGCAGCCA GGAAAACCGG CTGTGGATGT CCAGATGCAG 
AGCATGGCCC TGAGAGGTCC TGATGGGGAG CCCCAACCTG GGCTGGAGAG CCAGGGACGA 
GCAGCTTCCA TGCCCCGCCT GGCTGCAGAG ACTCAGCCGG CCCCAGACGC CAGCCCCATG 
AAGCGCTCCA TCTCCACCCT GGCGCCCCAA CGCCCCCATG TGGCTCATCT CTGCAACGCA 
GCCCTGGACC GAGCACCAGC CAGCCAGGCT GTGCCCCACC ACCACCACCG CTGCCACCGC 
CGCAGGGACA GGAAGCAGAA GTCCCTGGAG AAGGGGGCCA GCCTGTCTGC AGACACAGAT 
GGTGCACCCA GCAGTGCTGC GGGGCCAGGG CCACCCCTGG GAGAGGGGCC CGCAGGCTGC 
CGGAGGGAGC GCAGGCAGGA GCGGGGTCGG TCCCAGGAGC GGAGGCAGCC CTCTTCCTCT 
TCCTCGGAGA AGCAGCGCTT CTACTCCTGT GACCGCTTTG GGGGCCGTGA GCCCTCACAG 
CCCAAACCAT CCCTCAGCAG CCACCCCACG TCACCGACAG CGGGGCAGGA GCCAGGACCC 
CCGAGACCGG GTGGTGGTTC AGTGAATGGG AGCCCCTTGT TGTCAACATC CGGTGCTAGC 
ACCCCTGGCC GCAGTGGGCG GAGGCAGCTC CCCCAGACTC CGCTGACTCC CCGTCCCAGC 
GTCACCTACA AGACGGCCAA CTCATCCCCT GTCCACTTTG TTGGGGCGCA GACCAGCCTC 
CCCACCTTCT CCCCTGGTCG GCTTAGTCGT GGCCTTTCTG AACACAACGC TCTGCTCCAG 
AGAGACCCCC TCAGCCAGCC CCTGGCCGCC AGCTCTAGAA TTGGCTCTGA CCCTTATTTG 
GGGCAGCGTC TGGACAGTGA GGACACTGCC TGCACTCAGC CTGAGGACAC ACTCACCTTT 
GAGGAGGCTG TGGCCACCAA CTCAGGCCGC TCCTCCCGGA CTTCCTACGT GTCCTCTCTG 
ACCTCCCAGT CCCACCCCCT CCGCCGCGTG CCCAACGGCT ACCACTGCAC TCTGGGACTC 
AGCTCGGGCG GCCGGGTTCG GCACAGCTAC CACCACCCTG ACCAAGACCA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972339.1
CDS	133..6672
Translation	MVRFGDELGSRYGGADGERSRDGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCITVNRLFFVMGEDKVI*KYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFSNSTDPDPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFPDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAAPQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPDERLRYATTRHLRPDMKTHLDRPLVVELGRDGPRVPAGGKARPEGAEAAEDLSELMALEATAQDQPSPGTVFMEPHGDLEASGTMGVGPVHALPSTCLQKVEEQPEDADNQRNVTRMGSQPSDLSATVHIPVTLTGPPGDTAAVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSPRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILVVYMLFMFIFAVIAVQLFKGKFFYCTDESKGLERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQDKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQIHQAAGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSASLSNGGAIQTQESGIKESVSWGTQRTQEVPCEARTPLERGHSTEIPVVQPGKPAVDVQMQSMALRGPDGEPQPGLESQGRAASMPRLAAETQPAPDASPMKRSISTLAPQRPHVAHLCNAALDRAPASQAVPHHHHRCHRRRDRKQKSLEKGASLSADTDGAPSSAAGPGPPLGEGPAGCRRERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPSQPKPSLSSHPTSPTAGQEPGPPRPGGGSVNGSPLLSTSGASTPGRSGRRQLPQTPLTPRPSVTYKTANSSPVHFVGAQTSLPTFSPGRLSRGLSEHNALLQRDPLSQPLAASSRIGSDPYLGQRLDSEDTACTQPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRVRHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_014116864.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116864.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCAGC CGCTATGGGG GCGCCGACGG GGAGCGGTCT 
CGGGACGGCG GGGCCGGCGG GGCGGGCGGC CCGGGCCCCG GGGGGCTGCA GCCGGGCCAG 
CGGGTGCTCT ACAAGCAGTC GATCGCACAA AGGGCGCGGA CCATGGCGCT GTACAACCCC 
ATCCCGGTCA AGCAGAACTG CATCACGGTG AACCGCTTGT TCTTCGTAAT GGGCGAAGAC 
AAAGTCATTT GAAAATACGC CAAGCGCATC ACCGAGTGGC CTCCGTTTGA GTACATGATC 
CTGGCCACTA TCATCGCCAA CTGTATCGTG CTGGCACTGG AGCAGCACCT TCCCGACGGG 
GACAAGACGC CCATGTCTGA GCGGCTGGAC GACACAGAGC CCTATTTTAT TGGCATCTTC 
TGCTTTGAGG CGGGGATCAA GATCATAGCT CTGGGCTTTG TCTTCCACAA GGGCTCCTAC 
CTGCGGAACG GCTGGAACGT CATGGACTTT GTGGTGGTTC TCACAGGGAT CCTGGCCACG 
GCTGGCACTG ACTTTGACCT GCGGACCCTG CGGGCTGTGC GCGTGCTCAG GCCCCTGAAG 
CTGGTGTCTG GGATTCCAAG TTTGCAGGTG GTGCTCAAGT CTATCATGAA GGCCATGGTT 
CCACTCCTGC AGATCGGGCT GCTTCTCTTC TTCGCCATCC TCATGTTCGC GATCATTGGC 
CTTGAGTTCT ACATGGGCAA ATTCCACAAG GCCTGTTTCT CCAACAGCAC AGATCCAGAT 
CCAGTGGGGG ACTTCCCCTG TGGCAAGGAG GCCCCGGCCC GGCTGTGTGA GGGTGACACT 
GAATGTCGGG AGTACTGGCC AGGGCCCAAC TTTGGCATCA CCAACTTTGA CAATATCCTG 
TTTGCCATCT TGACAGTATT CCAGTGCATC ACCATGGAGG GCTGGACTGA CATCCTCTAC 
AATACAAATG ATGCAGCTGG CAACACTTGG AACTGGCTCT ACTTCATCCC TCTCATCATC 
ATTGGCTCCT TCTTCATGCT CAACCTGGTG CTGGGTGTGC TCTCAGGAGA GTTTGCCAAG 
GAGCGTGAGA GGGTGGAGAA CCGCCGGGCA TTCCTGAAGC TCCGCCGCCA GCAGCAGATC 
GAGCGGGAGC TCAACGGGTA CCTGGAGTGG ATCTTCAAAG CCGAGGAAGT CATGCTGGCC 
GAGGAGGACA AGAATGCGGA GGAGAAGTCC CCCCTGGATG TGTTGAAGAG AGCAGCCACG 
AAGAAGAGCC GGAATGACCT GATCCATGCC GAGGAGGGAG AGGACCGGTT TCCTGACCTC 
TGTGCTGTTG GATCCCCCTT TGCCCGTGCC AGCCTCAAGA GCGGGAAGAC GGAGAGCTCG 
TCGTACTTCC GTAGGAAGGA GAAGATGTTC CGGTTCTTCA TCCGACGCAT GGTGAAGGCG 
CAGAGCTTCT ACTGGGTGGT GCTGTGTGTG GTGGCCCTGA ATACTCTGTG CGTGGCCATG 
GTGCACTACA ACCAGCCACA GCGGCTCACC ACGGCGCTGT ACTTTGCAGA GTTTGTTTTC 
CTGGGTCTCT TCCTCACAGA GATGTCATTG AAGATGTATG GCCTGGGGCC CAGAAGCTAC 
TTCCGATCTT CCTTCAACTG CTTTGACTTT GGGGTCATTG TGGGGAGCAT CTTTGAAGTG 
GTCTGGGCAG CCATCAAGCC AGGAACATCC TTTGGGATCA GTGTGCTGCG GGCACTGCGG 
TTGCTGAGGA TCTTCAAAGT CACAAAGTAC TGGAGCTCCC TGCGCAACCT GGTGGTATCT 
CTGCTAAACT CCATGAAGTC CATCATCAGC CTCCTCTTCC TGCTGTTCCT GTTCATCGTG 
GTCTTTGCTC TACTAGGAAT GCAGCTATTC GGGGGACAGT TTAACTTTAG AGATGAGACC 
CCGACAACCA ACTTTGACAC TTTCCCCGCG GCCATTCTCA CTGTCTTCCA GATCCTAACA 
GGAGAGGACT GGAATGCGGT GATGTATCAC GGGATCGAGT CACAAGGCGG AGTCAGCAAA 
GGCATGTTCT CCTCCTTTTA CTTCATCGTC CTGACGCTGT TTGGAAATTA CACGCTACTG 
AATGTCTTCC TGGCCATCGC CGTGGACAAC CTTGCCAATG CTCAGGAGCT GACCAAGGAC 
GAAGAGGAGA TGGAAGAAGC AGCCAATCAG AAGCTTGCTC TGCAAAAGGC CAAAGAAGTG 
GCTGAAGTCA GCCCCATGTC TGCTGCAAAC ATCTCCATCG CTGCTGCGCC GCAGAACTCG 
GCCAAGGCGC GCTCGGTGTG GGAGCAGCGG GCCAGCCAGC TGCGGCTGCA GAACCTGCGG 
GCCAGCTGCG AGGCGCTGTA CAGCGAGATG GACCCGGACG AGCGGCTGCG CTACGCCACC 
ACGCGCCACC TGCGCCCCGA CATGAAGACG CACCTGGACC GGCCGCTCGT GGTGGAGCTG 
GGCCGCGACG GGCCGCGGGT TCCCGCCGGG GGCAAGGCCC GGCCCGAGGG CGCGGAGGCC 
GCCGAGGACC TCTCAGAACT CATGGCACTG GAGGCCACAG CTCAGGATCA GCCCAGTCCA 
GGCACCGTCT TTATGGAGCC ACACGGTGAC CTAGAAGCCA GTGGGACGAT GGGTGTGGGC 
CCTGTACATG CATTGCCCAG CACTTGTCTA CAGAAGGTGG AGGAACAGCC AGAGGATGCA 
GATAATCAGC GGAATGTCAC ACGGATGGGC AGCCAACCCT CAGACCTGAG TGCCACTGTG 
CATATCCCAG TGACGCTGAC CGGTCCTCCT GGGGACACTG CGGCTGTTCC CAGTGGTAAT 
GTGGACCTGG AAAGTCAAGC CGAGGGGAAG AAGGAGGTGG AAGCTGACGA TGTGATGAGG 
AGCGGGCCCC GACCCATCGT CCCATACAGC TCTATGTTCT GTTTAAGCCC CACCAACCTG 
CTCCGCCGCT TCTGTCATTA CATCGTGACC ATGCGGTACT TCGAGATGGT CATTCTTGTG 
GTCATCGCCC TGAGCAGCAT TGCCCTGGCT GCCGAGGACC CAGTGCGGAC AGACTCGCCT 
AGGAACAACG CCCTGAAGTA TATGGACTAC ATTTTCACGG GCGTCTTCAC CTTTGAGATG 
GTGATAAAGA TGATTGATTT GGGTCTGCTG CTGCATCCTG GGGCCTACTT CCGGGACCTG 
TGGAATATTC TGGACTTCAT CGTGGTCAGT GGGGCCCTGG TGGCGTTTGC CTTCTCAGGA 
TCCAAAGGGA AAGACATCAG CACCATCAAG TCCCTGAGAG TCTTGCGTGT CCTGCGGCCC 
CTCAAGACTA TAAAACGGCT GCCTAAGCTC AAGGCCGTGT TTGACTGTGT GGTGAACTCC 
CTGAAGAACG TCCTCAACAT CCTGGTCGTC TACATGCTCT TCATGTTCAT TTTTGCTGTC 
ATTGCGGTGC AGCTCTTCAA AGGGAAGTTT TTCTACTGCA CGGATGAGTC CAAGGGGCTG 
GAGAGGGACT GCCGGGGTCA ATATTTGGAT TATGAGAAGG AGGAGGTGGA AGCTCAACCA 
CGGCAGTGGA AGAAATATGA TTTTCACTAT GACAACGTGC TCTGGGCTCT GCTGACACTG 
TTCACAGTGT CCACGGGAGA AGGGTGGCCC ATGGTCCTAA AACACTCTGT GGACGCCACC 
TATGAGGAGC AGGGCCCCAG CCCCGGGTAT CGCATGGAGC TGTCCATCTT CTATGTGGTC 
TACTTCGTGG TTTTCCCCTT CTTCTTTGTC AATATCTTTG TGGCCTTGAT CATCATCACC 
TTCCAGGAGC AGGGGGACAA GGTGATGTCT GAATGCAGCC TGGAGAAGAA TGAGAGGGCT 
TGCATCGACT TTGCCATCAG TGCCAAGCCC CTGACGCGAT ACATGCCCCA GGACAAGCAG 
TCATTTCAGT ACAAGACATG GACGTTTGTG GTCTCACCTC CCTTTGAGTA CTTCATCATG 
GCTATGATCG CCCTCAACAC AGTGGTGCTA ATGATGAAGT TCTATGATGC ACCGTATGAA 
TATGAACTAA TGTTGAAATG CCTGAACATT GTGTTCACAT CCATGTTCTC TATGGAATGC 
GTGCTGAAGA TCATCGCCTT CGGGGTGCTG AACTATTTCA GAGATGCCTG GAATGTCTTT 
GACTTTGTCA CTGTGTTGGG AAGTATTACT GATATTTTAG TAACAGAGAT TGCGGAAACG 
AACAATTTCA TCAACCTCAG CTTCCTCCGC CTCTTCCGTG CTGCCCGTCT CATCAAGCTG 
CTCCGCCAAG GCTACACCAT CCGCATCCTG CTGTGGACCT TCGTCCAGTC CTTCAAGGCC 
CTGCCCTACG TGTGTCTGCT CATCGCCATG CTGTTCTTCA TCTACGCCAT CATTGGCATG 
CAGGTGTTTG GGAATATTGC CCTAGATGAT GACACCAGCA TCAACCGGCA TAACAACTTC 
CGGACGTTTT TACAAGCCTT GATGCTATTG TTCAGGAGTG CCACGGGGGA GGCCTGGCAC 
GAGATCATGC TGTCTTGTCT CAGCAACCAG GCCTGTGATG AGCACGCCAA TGCCAGTGAG 
TGTGGGAGCG ACTTTGCCTA CTTTTACTTC GTCTCCTTCA TCTTCCTTTG CTCCTTTCTG 
ATGTTGAACC TCTTTGTGGC CGTGATCATG GACAATTTTG AGTACCTTAC ACGGGACTCT 
TCCATCCTAG GGCCTCACCA CCTGGACGAG TTTATCCGGG TCTGGGCTGA GTACGACCCG 
GCTGCGTGTG GGCGCATCAG TTACAATGAC ATGTTTGAGA TGCTGAAACA CATGTCCCCA 
CCCCTGGGGC TGGGGAAGAA ATGCCCTGCT CGAGTTGCAT ACAAGCGCCT GGTTCGTATG 
AACATGCCCA TCTCCAATGA GGATATGACT GTCCACTTCA CGTCCACGCT GATGGCCCTC 
ATTCGGACCG CACTGGAGAT CAAGCTGGCT CCAGCTGGCA CGAAGCAGCA TCAGTGTGAT 
GCAGAGTTGA GGAAGGAAAT TTCCTCTGTG TGGGCCAATC TGCCCCAGAA GACCCTGGAT 
TTACTGGTGC CACCCCATAA ACCCGATGAG ATGACAGTGG GAAAAGTGTA TGCAGCTCTG 
ATGATATTTG ACTTCTATAA ACAGAACAAG ACCACCAGAG ACCAGATTCA CCAGGCCGCG 
GGAGGCCTGT CCCAGATGGG CCCTGTGTCG CTGTTCCACC CTCTGAAGGC CACACTGGAG 
CAGACACAGC CAGCTGTGCT CCGAGGAGCC CGGGTTTTCC TTCGGCAGAA GAGCTCTGCG 
TCCCTCAGCA ATGGTGGGGC CATACAAACC CAAGAGAGTG GCATCAAGGA GTCTGTTTCT 
TGGGGCACTC AGAGGACCCA GGAGGTACCT TGTGAGGCAA GGACACCCCT GGAGCGTGGC 
CACTCTACAG AGATCCCAGT GGTGCAGCCA GGAAAACCGG CTGTGGATGT CCAGATGCAG 
AGCATGGCCC TGAGAGGTCC TGATGGGGAG CCCCAACCTG GGCTGGAGAG CCAGGGACGA 
GCAGCTTCCA TGCCCCGCCT GGCTGCAGAG ACTCAGCCGG CCCCAGACGC CAGCCCCATG 
AAGCGCTCCA TCTCCACCCT GGCGCCCCAA CGCCCCCATG TGGCTCATCT CTGCAACGCA 
GCCCTGGACC GAGCACCAGC CAGCCAGGCT GTGCCCCACC ACCACCACCG CTGCCACCGC 
CGCAGGGACA GGAAGCAGAA GTCCCTGGAG AAGGGGGCCA GCCTGTCTGC AGACACAGAT 
GGTGCACCCA GCAGTGCTGC GGGGCCAGGG CCACCCCTGG GAGAGGGGCC CGCAGGCTGC 
CGGAGGGAGC GCAGGCAGGA GCGGGGTCGG TCCCAGGAGC GGAGGCAGCC CTCTTCCTCT 
TCCTCGGAGA AGCAGCGCTT CTACTCCTGT GACCGCTTTG GGGGCCGTGA GCCCTCACAG 
CCCAAACCAT CCCTCAGCAG CCACCCCACG TCACCGACAG CGGGGCAGGA GCCAGGACCC 
CCGAGACCGG GTGGTGGTTC AGTGAATGGG AGCCCCTTGT TGTCAACATC CGGTGCTAGC 
ACCCCTGGCC GCAGTGGGCG GAGGCAGCTC CCCCAGACTC CGCTGACTCC CCGTCCCAGC 
GTCACCTACA AGACGGCCAA CTCATCCCCT GTCCACTTTG TTGGGGCGCA GACCAGCCTC 
CCCACCTTCT CCCCTGGTCG GCTTAGTCGT GGCCTTTCTG AACACAACGC TCTGCTCCAG 
AGAGACCCCC TCAGCCAGCC CCTGGCCGCC AGCTCTAGAA TTGGCTCTGA CCCTTATTTG 
GGGCAGCGTC TGGACAGTGA GGACACTGCC TGCACTCAGC CTGAGGACAC ACTCACCTTT 
GAGGAGGCTG TGGCCACCAA CTCAGGCCGC TCCTCCCGGA CTTCCTACGT GTCCTCTCTG 
ACCTCCCAGT CCCACCCCCT CCGCCGCGTG CCCAACGGCT ACCACTGCAC TCTGGGACTC 
AGCTCGGGCG GCCGGGTTCG GCACAGCTAC CACCACCCTG ACCAAGACCA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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CACNA1B cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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