MYH10 gene cDNA ORF clone, Canis lupus familiaris(Dog)

mRNA	Protein	Name
XM_014113596.1	XP_013969071.1	myosin-10 isoform X3
XM_014113595.1	XP_013969070.1	myosin-10 isoform X2
XM_014113597.2	XP_013969072.1	myosin-10 isoform X4
XM_014113599.1	XP_013969074.1	myosin-10 isoform X6
XM_014113595.2	XP_013969070.1	myosin-10 isoform X2
XM_014113597.1	XP_013969072.1	myosin-10 isoform X4
XM_005620039.3	XP_005620096.2	myosin-10 isoform X1
XM_014113599.2	XP_013969074.1	myosin-10 isoform X6
XM_014113594.1	XP_013969069.1	myosin-10 isoform X1
XM_014113594.2	XP_013969069.1	myosin-10 isoform X1
XM_014113596.2	XP_013969071.1	myosin-10 isoform X3
XM_014113598.1	XP_013969073.1	myosin-10 isoform X5
XM_014113598.2	XP_013969073.1	myosin-10 isoform X5
XM_005620039.2	XP_005620096.1	myosin-10 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe36458	XM_014113596.2 Latest version!	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe36459	XM_014113597.2 Latest version!	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe36460	XM_014113598.2 Latest version!	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe36461	XM_014113599.2 Latest version!	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe06305	XM_005620039.2 Latest version!	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe36456	XM_014113594.2 Latest version!	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe36456	XM_005620039.3 Latest version!	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe36457	XM_014113595.2 Latest version!	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCe36457	XM_014113595.1	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe36459	XM_014113597.1	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe36461	XM_014113599.1	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe36456	XM_014113594.1	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe36458	XM_014113596.1	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe36460	XM_014113598.1	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

MYH10 ( XM_014113596.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969071.1 Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe36458
Clone ID Related Accession (Same CDS sequence)	XM_014113596.1 , XM_014113596.2
Accession Version	XM_014113596.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	myosin-10 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC CTTTGGAAAT GCGAAGACTG TGAAAAATGA TAATTCATCT 
CGCTTTGGCA AGTTTATTCG AATCAACTTT GATGTAACTG GCTATATTGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAGTT GTTATCTGGA GCAGGAGAAC ACCTAAAGTC GGATTTGCTC 
CTCGAAGGAT TTAATAACTA CAGATTCCTC TCCAACGGCT ATATTCCCAT CCCGGGACAG 
CAAGACAAGG ACAACTTTCA GGAAACCATG GAAGCAATGC ACATCATGGG CTTCTCTCAC 
GAGGAGATTC TGTCAATGCT TAAAGTAGTG TCATCAGTGC TACAGTTCGG AAATATTTCT 
TTCAAAAAGG AGCGAAATAC TGATCAAGCA TCCATGCCAG AGAACACAGT TGCACAGAAA 
CTCTGCCATC TTCTTGGGAT GAACGTGATG GAGTTCACGC GGGCCATCCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA TGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCG 
GTAGAAGCAT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GTTGGCTCGT TCACCGTATC 
AACAAAGCTC TGGACAGGAC CAAGCGTCAG GGCGCATCTT TTATTGGAAT CCTGGATATC 
GCTGGATTCG AAATATTTGA GCTCAACTCG TTCGAGCAAC TGTGCATCAA CTACACCAAT 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAG CTTCATTGAC TTTGGCCTCG ACCTGCAGCC CTGCATCGAC 
CTCATCGAGA GACCCGCTAA CCCCCCTGGT GTGCTGGCCC TTTTGGATGA AGAGTGCTGG 
TTCCCGAAGG CCACGGACAA AACCTTTGTT GAAAAACTAG TTCAAGAGCA AGGCTCTCAC 
TCCAAGTTCC AGAAACCCCG GCAACTGAAA GACAGAGCTG ACTTCTGCAT CATCCATTAT 
GCGGGCAAGG TGGACTACAA GGCTGATGAG TGGCTGATGA AGAACATGGA CCCCCTGAAC 
GACAATGTGG CCACCCTCCT GCACCAGTCA TCTGACAGAT TCGTGGCAGA GCTTTGGAAG 
GATGAGATTC AGAATATTCA GAGAGCTTCT TTCTATGACA GTGTTTCTGG TCTTCATGAG 
CCACCAGTGG ACCGTATCGT GGGTCTGGAT CAGGTCACGG GCATGACGGA GACAGCCTTT 
GGCTCTGCGT ATAAAACCAA GAAGGGCATG TTTCGGACTG TCGGGCAGCT CTACAAAGAA 
TCCCTCACCA AGCTGATGGC AACTCTCCGG AACACCAACC CTAACTTCGT CCGCTGCATC 
ATTCCAAATC ACGAGAAGCG AGCTGGAAAA CTGGATCCGC ACCTAGTGCT TGATCAGCTT 
CGCTGTAACG GCGTTCTGGA AGGAATCAGG ATCTGTCGCC AGGGGTTCCC CAACCGGATA 
GTTTTCCAGG AATTCAGACA GAGATACGAG ATCCTAACTC CAAATGCTAT CCCTAAGGGT 
TTCATGGACG GCAAACAGGC CTGCGAACGA ATGATCCGGG CTTTAGAACT GGACCCAAAC 
TTGTACAGAA TCGGACAGAG CAAGATATTT TTCCGAGCTG GCGTGCTGGC ACACTTAGAG 
GAAGAGAGGG ATCTAAAGAT CACCGACATC ATTATCTTCT TCCAGGCTGT CTGCAGAGGC 
TACCTGGCCA GAAAGGCCTT CGCCAAGAAG CAGCAGCAGC TGAGCGCCCT GAAGGTCCTG 
CAGCGGAACT GCGCCGCATA CCTGAAGCTC CGGCACTGGC AGTGGTGGCG TGTCTTCACG 
AAGGTGAAGC CGCTCCTGCA AGTGACCCGC CAGGAGGAAG AGCTTCAGGC CAAAGACGAG 
GAGCTGCTGA AGGTGAAGGA GAGGCAGACG AAGGTGGAGG GCGAGCTGGA GGAAATGGAG 
AGGAAGCACC AGCAGCTCCT GGAAGAGAAG AACATCCTAA CAGAGCAGCT CCAAGCAGAG 
ACAGAGCTCT TTGCTGAGGC AGAAGAGATG AGAGCCAGGC TCGCCGCCAA GAAGCAGGAG 
CTGGAGGAGA TTCTCCATGA CTTGGAATCC AGGGTCGAAG AGGAAGAAGA ACGAAACCAA 
ATCCTCCAAA ATGAAAAGAA GAAAATGCAA GCTCACATTC AGGACCTAGA GGAGCAACTG 
GATGAGGAAG AAGGCGCTCG CCAGAAACTA CAGCTGGAAA AGGTGACAGC GGAAGCAAAA 
ATCAAGAAGA TGGAGGAGGA GATCCTGCTG CTTGAGGACC AGAATTCCAA ATTTATCAAA 
GAAAAGAAAC TCATGGAAGA TCGCATTGCA GAGTGTTCCT CTCAGCTGGC TGAGGAGGAA 
GAAAAGGCCA AAAACTTGGC CAAAATCAGG AATAAGCAGG AAGTGATGAT CTCAGATTTA 
GAAGAACGCT TGAAGAAGGA AGAGAAGACG CGTCAGGAGC TGGAGAAGGC CAAACGAAAA 
CTGGACGGTG AGACCACTGA CCTGCAGGAC CAGATCGCAG AGCTCCAGGC GCAGATCGAC 
GAGCTCAAGG TCCAGCTGGC CAAGAAGGAG GAGGAGCTGC AAGGCGCGCT GGCCAGGGGT 
GACGATGAGA CGCTCCATAA GAACAATGCC CTCAAGGTGG TGCGAGAGCT GCAAGCCCAA 
ATCGCCGAGC TTCAGGAGGA CTTTGAATCA GAGAAGGCTT CACGGAACAA GGCGGAGAAG 
CAGAAGAGGG ACCTGAGTGA GGAGCTGGAG GCCCTGAAAA CAGAGCTGGA GGACACGCTG 
GACACCACCG CGGCTCAGCA GGAACTTCGC ACGAAGCGTG AACAAGAAGT GGCAGAGCTG 
AAGAAGGCTC TGGAGGAGGA AACGAGGAGC CACGAGGCTC AAATCCAGGA CATGAGACAG 
CGACACGCCA CAGCCCTGGA GGAGCTGTCG GAGCAGCTGG AACAGGCAAA GAGGTTCAAA 
GCGAATCTGG AGAAGAACAA GCAGGGCCTG GAGACTGACA ACAAGGAGCT GGCGTGTGAG 
GTGAAGGTGC TACAGCAGGT CAAGGCTGAG TCTGAGCACA AGAGGAAGAA GCTCGACGCC 
CAGGTGCAGG AGCTGCACGC CAAGGTCTCA GAAGGAGACA GGCTCAGGGT GGAGCTGGCC 
GAGAAGGCAA ACAAATTACA GAATGAACTG GACAATGTCT CAAGTCTTCT GGAAGAAGCA 
GAGAAGAAGG GCATTAAGTT TGCTAAGGAC GCAGCTAGTC TTGAGTCTCA ACTACAGGAC 
ACTCAGGAGC TCCTTCAGGA GGAGACCCGC CAGAAGCTGA ACCTGAGCAG CCGCATCCGG 
CAGCTGGAGG AGGAGAAGAA CAGTCTCCAG GAGCAGCAGG AGGAGGAGGA GGAGGCCAGG 
AAAAACCTGG AAAAGCAGGT GTTGGTGCTG CAGTCCCAGC TCGCCGATAC CAAGAAGAAG 
GTAGATGATG ACTTAGGAAC CATCGAGAGC TTAGAAGAAG CCAGAAAGAA GCTTCTGAAA 
GATGTGGAGG CCCTGGGCCA GCGCCTGGAG GAGAAGGCTC TGGCCTATGA CAAGCTGGAG 
AAGACCAAGA ACCGCCTGCA GCAGGAGCTC GACGACCTGA CAGTGGACCT AGATCACCAG 
CGCCAGATCG CCTCCAACTT GGAGAAGAAG CAGAAGAAGT TTGACCAGCT GCTGGCAGAG 
GAGAAGAACA TCTCCGCCCG CTATGCAGAA GAGCGAGACC GCGCAGAGGC AGAGGCCAGG 
GAGAAGGAAA CCAAAGCCCT GTCCCTGGCC CGAGCCCTCG AGGAGGCCCT AGAAACCAAG 
GAAGAGTTTG AGAGACAGAA CAAGCAGCTG CGGGCAGACA TGGAGGACCT GATAAGCTCC 
AAGGATGACG TGGGGAAGAA CGTGCACGAG CTTGAAAAAT CGAAGCGGGC CCTGGAGCAA 
CAGGTGGAGG AGATGAGGAC ACAGCTGGAA GAGCTGGAGG ATGAGCTTCA GGCCACAGAA 
GACGCCAAGC TTCGCCTGGA AGTCAACATG CAGGCCATGA AGGCCCAGTT TGAGAGGGAC 
TTACAAACCA GGGACGAGCA GAATGAAGAG AAGAAGCGGC TCCTGGTTAA GCAGGTGCGG 
GAGCTGGAGG CAGAGCTGGA AGATGAGCGG AAGCAGCGAG CGCTGGCGGT CGCTTCTAAA 
AAGAAACTGG AAATAGACCT GAAGGACCTT GAAGCCCAGA TTGAGGCTGC GAACAAAGCG 
AGGGACGAAG TGATCAAGCA GCTTCGCAAG CTTCAGGCTC AGATGAAGGA TTACCAACGT 
GAATTAGAAG AAGCCCGTGC ATCCCGGGAT GAGATTTTTG CTCAATCCAA AGAGAGTGAA 
AAGAAACTGA AGAGTCTGGA AGCAGAAATC CTTCAATTGC AGGAGGAACT GGCCTCATCT 
GAGCGAGCGC GCCGCCACGC CGAGCAGGAG AGAGATGAGC TGGCTGACGA GATCGCCAAC 
AGCGCCTCTG GCAAGTCTGC GCTGCTGGAT GAGAAGCGGC GTCTGGAGGC ACGGATCGCG 
CAGCTGGAGG AGGAGCTGGA AGAGGAGCAG AGCAACATGG AGCTGCTCAA CGACCGCTTC 
CGCAAGACCA CGCTGCAGGT GGACACGCTG AACGCCGAGC TCGCGGCTGA GCGCAGCGCC 
GCCCAGAAGA GCGACAACGC ACGCCAGCAG CTAGAGCGGC AGAACAAGGA GCTGAAGGCC 
AAGCTGCAGG AACTGGAGGG CGCCGTCAAG TCCAAGTTCA AGGCCACCAT CTCTGCCCTG 
GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG CTGGAGCAGG AAGCCAAGGA ACGAGCAGCC 
GCCAACAAAC TAGTCCGTCG CACGGAGAAA AAGCTGAAAG AGATCTTCAT GCAGGTTGAA 
GATGAGCGTC GACATGCGGA TCAGTATAAA GAACAGATGG AGAAGGCCAA CGCCAGGATG 
AAACAGCTCA AGCGGCAGCT GGAGGAGGCA GAGGAGGAAG CCACACGTGC CAACGCATCT 
CGGCGTAAGC TCCAGCGGGA ACTGGACGAT GCCACGGAGG CCAACGAGGG CCTGAGCCGT 
GAGGTCAGCA CGCTGAAGAA CCGGCTGAGA CGAGGTGGCC CCATAAGCTT CTCGTCCAGC 
CGATCGGGCC GGCGCCAGCT ACACATCGAG GGAGCCTCCC TGGAGCTGTC AGACGATGAC 
ACGGAGAGTA AGACCAGTGA CGTCAACGAG ACACAGCCGC CGCAGTCGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969071.1
CDS	132..6125
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_014113596.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113596.1

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC CTTTGGAAAT GCGAAGACTG TGAAAAATGA TAATTCATCT 
CGCTTTGGCA AGTTTATTCG AATCAACTTT GATGTAACTG GCTATATTGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAGTT GTTATCTGGA GCAGGAGAAC ACCTAAAGTC GGATTTGCTC 
CTCGAAGGAT TTAATAACTA CAGATTCCTC TCCAACGGCT ATATTCCCAT CCCGGGACAG 
CAAGACAAGG ACAACTTTCA GGAAACCATG GAAGCAATGC ACATCATGGG CTTCTCTCAC 
GAGGAGATTC TGTCAATGCT TAAAGTAGTG TCATCAGTGC TACAGTTCGG AAATATTTCT 
TTCAAAAAGG AGCGAAATAC TGATCAAGCA TCCATGCCAG AGAACACAGT TGCACAGAAA 
CTCTGCCATC TTCTTGGGAT GAACGTGATG GAGTTCACGC GGGCCATCCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA TGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCG 
GTAGAAGCAT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GTTGGCTCGT TCACCGTATC 
AACAAAGCTC TGGACAGGAC CAAGCGTCAG GGCGCATCTT TTATTGGAAT CCTGGATATC 
GCTGGATTCG AAATATTTGA GCTCAACTCG TTCGAGCAAC TGTGCATCAA CTACACCAAT 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAG CTTCATTGAC TTTGGCCTCG ACCTGCAGCC CTGCATCGAC 
CTCATCGAGA GACCCGCTAA CCCCCCTGGT GTGCTGGCCC TTTTGGATGA AGAGTGCTGG 
TTCCCGAAGG CCACGGACAA AACCTTTGTT GAAAAACTAG TTCAAGAGCA AGGCTCTCAC 
TCCAAGTTCC AGAAACCCCG GCAACTGAAA GACAGAGCTG ACTTCTGCAT CATCCATTAT 
GCGGGCAAGG TGGACTACAA GGCTGATGAG TGGCTGATGA AGAACATGGA CCCCCTGAAC 
GACAATGTGG CCACCCTCCT GCACCAGTCA TCTGACAGAT TCGTGGCAGA GCTTTGGAAG 
GATGAGATTC AGAATATTCA GAGAGCTTCT TTCTATGACA GTGTTTCTGG TCTTCATGAG 
CCACCAGTGG ACCGTATCGT GGGTCTGGAT CAGGTCACGG GCATGACGGA GACAGCCTTT 
GGCTCTGCGT ATAAAACCAA GAAGGGCATG TTTCGGACTG TCGGGCAGCT CTACAAAGAA 
TCCCTCACCA AGCTGATGGC AACTCTCCGG AACACCAACC CTAACTTCGT CCGCTGCATC 
ATTCCAAATC ACGAGAAGCG AGCTGGAAAA CTGGATCCGC ACCTAGTGCT TGATCAGCTT 
CGCTGTAACG GCGTTCTGGA AGGAATCAGG ATCTGTCGCC AGGGGTTCCC CAACCGGATA 
GTTTTCCAGG AATTCAGACA GAGATACGAG ATCCTAACTC CAAATGCTAT CCCTAAGGGT 
TTCATGGACG GCAAACAGGC CTGCGAACGA ATGATCCGGG CTTTAGAACT GGACCCAAAC 
TTGTACAGAA TCGGACAGAG CAAGATATTT TTCCGAGCTG GCGTGCTGGC ACACTTAGAG 
GAAGAGAGGG ATCTAAAGAT CACCGACATC ATTATCTTCT TCCAGGCTGT CTGCAGAGGC 
TACCTGGCCA GAAAGGCCTT CGCCAAGAAG CAGCAGCAGC TGAGCGCCCT GAAGGTCCTG 
CAGCGGAACT GCGCCGCATA CCTGAAGCTC CGGCACTGGC AGTGGTGGCG TGTCTTCACG 
AAGGTGAAGC CGCTCCTGCA AGTGACCCGC CAGGAGGAAG AGCTTCAGGC CAAAGACGAG 
GAGCTGCTGA AGGTGAAGGA GAGGCAGACG AAGGTGGAGG GCGAGCTGGA GGAAATGGAG 
AGGAAGCACC AGCAGCTCCT GGAAGAGAAG AACATCCTAA CAGAGCAGCT CCAAGCAGAG 
ACAGAGCTCT TTGCTGAGGC AGAAGAGATG AGAGCCAGGC TCGCCGCCAA GAAGCAGGAG 
CTGGAGGAGA TTCTCCATGA CTTGGAATCC AGGGTCGAAG AGGAAGAAGA ACGAAACCAA 
ATCCTCCAAA ATGAAAAGAA GAAAATGCAA GCTCACATTC AGGACCTAGA GGAGCAACTG 
GATGAGGAAG AAGGCGCTCG CCAGAAACTA CAGCTGGAAA AGGTGACAGC GGAAGCAAAA 
ATCAAGAAGA TGGAGGAGGA GATCCTGCTG CTTGAGGACC AGAATTCCAA ATTTATCAAA 
GAAAAGAAAC TCATGGAAGA TCGCATTGCA GAGTGTTCCT CTCAGCTGGC TGAGGAGGAA 
GAAAAGGCCA AAAACTTGGC CAAAATCAGG AATAAGCAGG AAGTGATGAT CTCAGATTTA 
GAAGAACGCT TGAAGAAGGA AGAGAAGACG CGTCAGGAGC TGGAGAAGGC CAAACGAAAA 
CTGGACGGTG AGACCACTGA CCTGCAGGAC CAGATCGCAG AGCTCCAGGC GCAGATCGAC 
GAGCTCAAGG TCCAGCTGGC CAAGAAGGAG GAGGAGCTGC AAGGCGCGCT GGCCAGGGGT 
GACGATGAGA CGCTCCATAA GAACAATGCC CTCAAGGTGG TGCGAGAGCT GCAAGCCCAA 
ATCGCCGAGC TTCAGGAGGA CTTTGAATCA GAGAAGGCTT CACGGAACAA GGCGGAGAAG 
CAGAAGAGGG ACCTGAGTGA GGAGCTGGAG GCCCTGAAAA CAGAGCTGGA GGACACGCTG 
GACACCACCG CGGCTCAGCA GGAACTTCGC ACGAAGCGTG AACAAGAAGT GGCAGAGCTG 
AAGAAGGCTC TGGAGGAGGA AACGAGGAGC CACGAGGCTC AAATCCAGGA CATGAGACAG 
CGACACGCCA CAGCCCTGGA GGAGCTGTCG GAGCAGCTGG AACAGGCAAA GAGGTTCAAA 
GCGAATCTGG AGAAGAACAA GCAGGGCCTG GAGACTGACA ACAAGGAGCT GGCGTGTGAG 
GTGAAGGTGC TACAGCAGGT CAAGGCTGAG TCTGAGCACA AGAGGAAGAA GCTCGACGCC 
CAGGTGCAGG AGCTGCACGC CAAGGTCTCA GAAGGAGACA GGCTCAGGGT GGAGCTGGCC 
GAGAAGGCAA ACAAATTACA GAATGAACTG GACAATGTCT CAAGTCTTCT GGAAGAAGCA 
GAGAAGAAGG GCATTAAGTT TGCTAAGGAC GCAGCTAGTC TTGAGTCTCA ACTACAGGAC 
ACTCAGGAGC TCCTTCAGGA GGAGACCCGC CAGAAGCTGA ACCTGAGCAG CCGCATCCGG 
CAGCTGGAGG AGGAGAAGAA CAGTCTCCAG GAGCAGCAGG AGGAGGAGGA GGAGGCCAGG 
AAAAACCTGG AAAAGCAGGT GTTGGTGCTG CAGTCCCAGC TCGCCGATAC CAAGAAGAAG 
GTAGATGATG ACTTAGGAAC CATCGAGAGC TTAGAAGAAG CCAGAAAGAA GCTTCTGAAA 
GATGTGGAGG CCCTGGGCCA GCGCCTGGAG GAGAAGGCTC TGGCCTATGA CAAGCTGGAG 
AAGACCAAGA ACCGCCTGCA GCAGGAGCTC GACGACCTGA CAGTGGACCT AGATCACCAG 
CGCCAGATCG CCTCCAACTT GGAGAAGAAG CAGAAGAAGT TTGACCAGCT GCTGGCAGAG 
GAGAAGAACA TCTCCGCCCG CTATGCAGAA GAGCGAGACC GCGCAGAGGC AGAGGCCAGG 
GAGAAGGAAA CCAAAGCCCT GTCCCTGGCC CGAGCCCTCG AGGAGGCCCT AGAAACCAAG 
GAAGAGTTTG AGAGACAGAA CAAGCAGCTG CGGGCAGACA TGGAGGACCT GATAAGCTCC 
AAGGATGACG TGGGGAAGAA CGTGCACGAG CTTGAAAAAT CGAAGCGGGC CCTGGAGCAA 
CAGGTGGAGG AGATGAGGAC ACAGCTGGAA GAGCTGGAGG ATGAGCTTCA GGCCACAGAA 
GACGCCAAGC TTCGCCTGGA AGTCAACATG CAGGCCATGA AGGCCCAGTT TGAGAGGGAC 
TTACAAACCA GGGACGAGCA GAATGAAGAG AAGAAGCGGC TCCTGGTTAA GCAGGTGCGG 
GAGCTGGAGG CAGAGCTGGA AGATGAGCGG AAGCAGCGAG CGCTGGCGGT CGCTTCTAAA 
AAGAAACTGG AAATAGACCT GAAGGACCTT GAAGCCCAGA TTGAGGCTGC GAACAAAGCG 
AGGGACGAAG TGATCAAGCA GCTTCGCAAG CTTCAGGCTC AGATGAAGGA TTACCAACGT 
GAATTAGAAG AAGCCCGTGC ATCCCGGGAT GAGATTTTTG CTCAATCCAA AGAGAGTGAA 
AAGAAACTGA AGAGTCTGGA AGCAGAAATC CTTCAATTGC AGGAGGAACT GGCCTCATCT 
GAGCGAGCGC GCCGCCACGC CGAGCAGGAG AGAGATGAGC TGGCTGACGA GATCGCCAAC 
AGCGCCTCTG GCAAGTCTGC GCTGCTGGAT GAGAAGCGGC GTCTGGAGGC ACGGATCGCG 
CAGCTGGAGG AGGAGCTGGA AGAGGAGCAG AGCAACATGG AGCTGCTCAA CGACCGCTTC 
CGCAAGACCA CGCTGCAGGT GGACACGCTG AACGCCGAGC TCGCGGCTGA GCGCAGCGCC 
GCCCAGAAGA GCGACAACGC ACGCCAGCAG CTAGAGCGGC AGAACAAGGA GCTGAAGGCC 
AAGCTGCAGG AACTGGAGGG CGCCGTCAAG TCCAAGTTCA AGGCCACCAT CTCTGCCCTG 
GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG CTGGAGCAGG AAGCCAAGGA ACGAGCAGCC 
GCCAACAAAC TAGTCCGTCG CACGGAGAAA AAGCTGAAAG AGATCTTCAT GCAGGTTGAA 
GATGAGCGTC GACATGCGGA TCAGTATAAA GAACAGATGG AGAAGGCCAA CGCCAGGATG 
AAACAGCTCA AGCGGCAGCT GGAGGAGGCA GAGGAGGAAG CCACACGTGC CAACGCATCT 
CGGCGTAAGC TCCAGCGGGA ACTGGACGAT GCCACGGAGG CCAACGAGGG CCTGAGCCGT 
GAGGTCAGCA CGCTGAAGAA CCGGCTGAGA CGAGGTGGCC CCATAAGCTT CTCGTCCAGC 
CGATCGGGCC GGCGCCAGCT ACACATCGAG GGAGCCTCCC TGGAGCTGTC AGACGATGAC 
ACGGAGAGTA AGACCAGTGA CGTCAACGAG ACACAGCCGC CGCAGTCGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_014113596.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969071.1 Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X3, mRNA.

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CloneID	OCe36458
Clone ID Related Accession (Same CDS sequence)	XM_014113596.1 , XM_014113596.2
Accession Version	XM_014113596.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	myosin-10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113596.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC CTTTGGAAAT GCGAAGACTG TGAAAAATGA TAATTCATCT 
CGCTTTGGCA AGTTTATTCG AATCAACTTT GATGTAACTG GCTATATTGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAGTT GTTATCTGGA GCAGGAGAAC ACCTAAAGTC GGATTTGCTC 
CTCGAAGGAT TTAATAACTA CAGATTCCTC TCCAACGGCT ATATTCCCAT CCCGGGACAG 
CAAGACAAGG ACAACTTTCA GGAAACCATG GAAGCAATGC ACATCATGGG CTTCTCTCAC 
GAGGAGATTC TGTCAATGCT TAAAGTAGTG TCATCAGTGC TACAGTTCGG AAATATTTCT 
TTCAAAAAGG AGCGAAATAC TGATCAAGCA TCCATGCCAG AGAACACAGT TGCACAGAAA 
CTCTGCCATC TTCTTGGGAT GAACGTGATG GAGTTCACGC GGGCCATCCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA TGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCG 
GTAGAAGCAT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GTTGGCTCGT TCACCGTATC 
AACAAAGCTC TGGACAGGAC CAAGCGTCAG GGCGCATCTT TTATTGGAAT CCTGGATATC 
GCTGGATTCG AAATATTTGA GCTCAACTCG TTCGAGCAAC TGTGCATCAA CTACACCAAT 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAG CTTCATTGAC TTTGGCCTCG ACCTGCAGCC CTGCATCGAC 
CTCATCGAGA GACCCGCTAA CCCCCCTGGT GTGCTGGCCC TTTTGGATGA AGAGTGCTGG 
TTCCCGAAGG CCACGGACAA AACCTTTGTT GAAAAACTAG TTCAAGAGCA AGGCTCTCAC 
TCCAAGTTCC AGAAACCCCG GCAACTGAAA GACAGAGCTG ACTTCTGCAT CATCCATTAT 
GCGGGCAAGG TGGACTACAA GGCTGATGAG TGGCTGATGA AGAACATGGA CCCCCTGAAC 
GACAATGTGG CCACCCTCCT GCACCAGTCA TCTGACAGAT TCGTGGCAGA GCTTTGGAAG 
GATGAGATTC AGAATATTCA GAGAGCTTCT TTCTATGACA GTGTTTCTGG TCTTCATGAG 
CCACCAGTGG ACCGTATCGT GGGTCTGGAT CAGGTCACGG GCATGACGGA GACAGCCTTT 
GGCTCTGCGT ATAAAACCAA GAAGGGCATG TTTCGGACTG TCGGGCAGCT CTACAAAGAA 
TCCCTCACCA AGCTGATGGC AACTCTCCGG AACACCAACC CTAACTTCGT CCGCTGCATC 
ATTCCAAATC ACGAGAAGCG AGCTGGAAAA CTGGATCCGC ACCTAGTGCT TGATCAGCTT 
CGCTGTAACG GCGTTCTGGA AGGAATCAGG ATCTGTCGCC AGGGGTTCCC CAACCGGATA 
GTTTTCCAGG AATTCAGACA GAGATACGAG ATCCTAACTC CAAATGCTAT CCCTAAGGGT 
TTCATGGACG GCAAACAGGC CTGCGAACGA ATGATCCGGG CTTTAGAACT GGACCCAAAC 
TTGTACAGAA TCGGACAGAG CAAGATATTT TTCCGAGCTG GCGTGCTGGC ACACTTAGAG 
GAAGAGAGGG ATCTAAAGAT CACCGACATC ATTATCTTCT TCCAGGCTGT CTGCAGAGGC 
TACCTGGCCA GAAAGGCCTT CGCCAAGAAG CAGCAGCAGC TGAGCGCCCT GAAGGTCCTG 
CAGCGGAACT GCGCCGCATA CCTGAAGCTC CGGCACTGGC AGTGGTGGCG TGTCTTCACG 
AAGGTGAAGC CGCTCCTGCA AGTGACCCGC CAGGAGGAAG AGCTTCAGGC CAAAGACGAG 
GAGCTGCTGA AGGTGAAGGA GAGGCAGACG AAGGTGGAGG GCGAGCTGGA GGAAATGGAG 
AGGAAGCACC AGCAGCTCCT GGAAGAGAAG AACATCCTAA CAGAGCAGCT CCAAGCAGAG 
ACAGAGCTCT TTGCTGAGGC AGAAGAGATG AGAGCCAGGC TCGCCGCCAA GAAGCAGGAG 
CTGGAGGAGA TTCTCCATGA CTTGGAATCC AGGGTCGAAG AGGAAGAAGA ACGAAACCAA 
ATCCTCCAAA ATGAAAAGAA GAAAATGCAA GCTCACATTC AGGACCTAGA GGAGCAACTG 
GATGAGGAAG AAGGCGCTCG CCAGAAACTA CAGCTGGAAA AGGTGACAGC GGAAGCAAAA 
ATCAAGAAGA TGGAGGAGGA GATCCTGCTG CTTGAGGACC AGAATTCCAA ATTTATCAAA 
GAAAAGAAAC TCATGGAAGA TCGCATTGCA GAGTGTTCCT CTCAGCTGGC TGAGGAGGAA 
GAAAAGGCCA AAAACTTGGC CAAAATCAGG AATAAGCAGG AAGTGATGAT CTCAGATTTA 
GAAGAACGCT TGAAGAAGGA AGAGAAGACG CGTCAGGAGC TGGAGAAGGC CAAACGAAAA 
CTGGACGGTG AGACCACTGA CCTGCAGGAC CAGATCGCAG AGCTCCAGGC GCAGATCGAC 
GAGCTCAAGG TCCAGCTGGC CAAGAAGGAG GAGGAGCTGC AAGGCGCGCT GGCCAGGGGT 
GACGATGAGA CGCTCCATAA GAACAATGCC CTCAAGGTGG TGCGAGAGCT GCAAGCCCAA 
ATCGCCGAGC TTCAGGAGGA CTTTGAATCA GAGAAGGCTT CACGGAACAA GGCGGAGAAG 
CAGAAGAGGG ACCTGAGTGA GGAGCTGGAG GCCCTGAAAA CAGAGCTGGA GGACACGCTG 
GACACCACCG CGGCTCAGCA GGAACTTCGC ACGAAGCGTG AACAAGAAGT GGCAGAGCTG 
AAGAAGGCTC TGGAGGAGGA AACGAGGAGC CACGAGGCTC AAATCCAGGA CATGAGACAG 
CGACACGCCA CAGCCCTGGA GGAGCTGTCG GAGCAGCTGG AACAGGCAAA GAGGTTCAAA 
GCGAATCTGG AGAAGAACAA GCAGGGCCTG GAGACTGACA ACAAGGAGCT GGCGTGTGAG 
GTGAAGGTGC TACAGCAGGT CAAGGCTGAG TCTGAGCACA AGAGGAAGAA GCTCGACGCC 
CAGGTGCAGG AGCTGCACGC CAAGGTCTCA GAAGGAGACA GGCTCAGGGT GGAGCTGGCC 
GAGAAGGCAA ACAAATTACA GAATGAACTG GACAATGTCT CAAGTCTTCT GGAAGAAGCA 
GAGAAGAAGG GCATTAAGTT TGCTAAGGAC GCAGCTAGTC TTGAGTCTCA ACTACAGGAC 
ACTCAGGAGC TCCTTCAGGA GGAGACCCGC CAGAAGCTGA ACCTGAGCAG CCGCATCCGG 
CAGCTGGAGG AGGAGAAGAA CAGTCTCCAG GAGCAGCAGG AGGAGGAGGA GGAGGCCAGG 
AAAAACCTGG AAAAGCAGGT GTTGGTGCTG CAGTCCCAGC TCGCCGATAC CAAGAAGAAG 
GTAGATGATG ACTTAGGAAC CATCGAGAGC TTAGAAGAAG CCAGAAAGAA GCTTCTGAAA 
GATGTGGAGG CCCTGGGCCA GCGCCTGGAG GAGAAGGCTC TGGCCTATGA CAAGCTGGAG 
AAGACCAAGA ACCGCCTGCA GCAGGAGCTC GACGACCTGA CAGTGGACCT AGATCACCAG 
CGCCAGATCG CCTCCAACTT GGAGAAGAAG CAGAAGAAGT TTGACCAGCT GCTGGCAGAG 
GAGAAGAACA TCTCCGCCCG CTATGCAGAA GAGCGAGACC GCGCAGAGGC AGAGGCCAGG 
GAGAAGGAAA CCAAAGCCCT GTCCCTGGCC CGAGCCCTCG AGGAGGCCCT AGAAACCAAG 
GAAGAGTTTG AGAGACAGAA CAAGCAGCTG CGGGCAGACA TGGAGGACCT GATAAGCTCC 
AAGGATGACG TGGGGAAGAA CGTGCACGAG CTTGAAAAAT CGAAGCGGGC CCTGGAGCAA 
CAGGTGGAGG AGATGAGGAC ACAGCTGGAA GAGCTGGAGG ATGAGCTTCA GGCCACAGAA 
GACGCCAAGC TTCGCCTGGA AGTCAACATG CAGGCCATGA AGGCCCAGTT TGAGAGGGAC 
TTACAAACCA GGGACGAGCA GAATGAAGAG AAGAAGCGGC TCCTGGTTAA GCAGGTGCGG 
GAGCTGGAGG CAGAGCTGGA AGATGAGCGG AAGCAGCGAG CGCTGGCGGT CGCTTCTAAA 
AAGAAACTGG AAATAGACCT GAAGGACCTT GAAGCCCAGA TTGAGGCTGC GAACAAAGCG 
AGGGACGAAG TGATCAAGCA GCTTCGCAAG CTTCAGGCTC AGATGAAGGA TTACCAACGT 
GAATTAGAAG AAGCCCGTGC ATCCCGGGAT GAGATTTTTG CTCAATCCAA AGAGAGTGAA 
AAGAAACTGA AGAGTCTGGA AGCAGAAATC CTTCAATTGC AGGAGGAACT GGCCTCATCT 
GAGCGAGCGC GCCGCCACGC CGAGCAGGAG AGAGATGAGC TGGCTGACGA GATCGCCAAC 
AGCGCCTCTG GCAAGTCTGC GCTGCTGGAT GAGAAGCGGC GTCTGGAGGC ACGGATCGCG 
CAGCTGGAGG AGGAGCTGGA AGAGGAGCAG AGCAACATGG AGCTGCTCAA CGACCGCTTC 
CGCAAGACCA CGCTGCAGGT GGACACGCTG AACGCCGAGC TCGCGGCTGA GCGCAGCGCC 
GCCCAGAAGA GCGACAACGC ACGCCAGCAG CTAGAGCGGC AGAACAAGGA GCTGAAGGCC 
AAGCTGCAGG AACTGGAGGG CGCCGTCAAG TCCAAGTTCA AGGCCACCAT CTCTGCCCTG 
GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG CTGGAGCAGG AAGCCAAGGA ACGAGCAGCC 
GCCAACAAAC TAGTCCGTCG CACGGAGAAA AAGCTGAAAG AGATCTTCAT GCAGGTTGAA 
GATGAGCGTC GACATGCGGA TCAGTATAAA GAACAGATGG AGAAGGCCAA CGCCAGGATG 
AAACAGCTCA AGCGGCAGCT GGAGGAGGCA GAGGAGGAAG CCACACGTGC CAACGCATCT 
CGGCGTAAGC TCCAGCGGGA ACTGGACGAT GCCACGGAGG CCAACGAGGG CCTGAGCCGT 
GAGGTCAGCA CGCTGAAGAA CCGGCTGAGA CGAGGTGGCC CCATAAGCTT CTCGTCCAGC 
CGATCGGGCC GGCGCCAGCT ACACATCGAG GGAGCCTCCC TGGAGCTGTC AGACGATGAC 
ACGGAGAGTA AGACCAGTGA CGTCAACGAG ACACAGCCGC CGCAGTCGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969071.1
CDS	192..6185
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_014113596.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113596.2

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC CTTTGGAAAT GCGAAGACTG TGAAAAATGA TAATTCATCT 
CGCTTTGGCA AGTTTATTCG AATCAACTTT GATGTAACTG GCTATATTGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAGTT GTTATCTGGA GCAGGAGAAC ACCTAAAGTC GGATTTGCTC 
CTCGAAGGAT TTAATAACTA CAGATTCCTC TCCAACGGCT ATATTCCCAT CCCGGGACAG 
CAAGACAAGG ACAACTTTCA GGAAACCATG GAAGCAATGC ACATCATGGG CTTCTCTCAC 
GAGGAGATTC TGTCAATGCT TAAAGTAGTG TCATCAGTGC TACAGTTCGG AAATATTTCT 
TTCAAAAAGG AGCGAAATAC TGATCAAGCA TCCATGCCAG AGAACACAGT TGCACAGAAA 
CTCTGCCATC TTCTTGGGAT GAACGTGATG GAGTTCACGC GGGCCATCCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA TGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCG 
GTAGAAGCAT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GTTGGCTCGT TCACCGTATC 
AACAAAGCTC TGGACAGGAC CAAGCGTCAG GGCGCATCTT TTATTGGAAT CCTGGATATC 
GCTGGATTCG AAATATTTGA GCTCAACTCG TTCGAGCAAC TGTGCATCAA CTACACCAAT 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAG CTTCATTGAC TTTGGCCTCG ACCTGCAGCC CTGCATCGAC 
CTCATCGAGA GACCCGCTAA CCCCCCTGGT GTGCTGGCCC TTTTGGATGA AGAGTGCTGG 
TTCCCGAAGG CCACGGACAA AACCTTTGTT GAAAAACTAG TTCAAGAGCA AGGCTCTCAC 
TCCAAGTTCC AGAAACCCCG GCAACTGAAA GACAGAGCTG ACTTCTGCAT CATCCATTAT 
GCGGGCAAGG TGGACTACAA GGCTGATGAG TGGCTGATGA AGAACATGGA CCCCCTGAAC 
GACAATGTGG CCACCCTCCT GCACCAGTCA TCTGACAGAT TCGTGGCAGA GCTTTGGAAG 
GATGAGATTC AGAATATTCA GAGAGCTTCT TTCTATGACA GTGTTTCTGG TCTTCATGAG 
CCACCAGTGG ACCGTATCGT GGGTCTGGAT CAGGTCACGG GCATGACGGA GACAGCCTTT 
GGCTCTGCGT ATAAAACCAA GAAGGGCATG TTTCGGACTG TCGGGCAGCT CTACAAAGAA 
TCCCTCACCA AGCTGATGGC AACTCTCCGG AACACCAACC CTAACTTCGT CCGCTGCATC 
ATTCCAAATC ACGAGAAGCG AGCTGGAAAA CTGGATCCGC ACCTAGTGCT TGATCAGCTT 
CGCTGTAACG GCGTTCTGGA AGGAATCAGG ATCTGTCGCC AGGGGTTCCC CAACCGGATA 
GTTTTCCAGG AATTCAGACA GAGATACGAG ATCCTAACTC CAAATGCTAT CCCTAAGGGT 
TTCATGGACG GCAAACAGGC CTGCGAACGA ATGATCCGGG CTTTAGAACT GGACCCAAAC 
TTGTACAGAA TCGGACAGAG CAAGATATTT TTCCGAGCTG GCGTGCTGGC ACACTTAGAG 
GAAGAGAGGG ATCTAAAGAT CACCGACATC ATTATCTTCT TCCAGGCTGT CTGCAGAGGC 
TACCTGGCCA GAAAGGCCTT CGCCAAGAAG CAGCAGCAGC TGAGCGCCCT GAAGGTCCTG 
CAGCGGAACT GCGCCGCATA CCTGAAGCTC CGGCACTGGC AGTGGTGGCG TGTCTTCACG 
AAGGTGAAGC CGCTCCTGCA AGTGACCCGC CAGGAGGAAG AGCTTCAGGC CAAAGACGAG 
GAGCTGCTGA AGGTGAAGGA GAGGCAGACG AAGGTGGAGG GCGAGCTGGA GGAAATGGAG 
AGGAAGCACC AGCAGCTCCT GGAAGAGAAG AACATCCTAA CAGAGCAGCT CCAAGCAGAG 
ACAGAGCTCT TTGCTGAGGC AGAAGAGATG AGAGCCAGGC TCGCCGCCAA GAAGCAGGAG 
CTGGAGGAGA TTCTCCATGA CTTGGAATCC AGGGTCGAAG AGGAAGAAGA ACGAAACCAA 
ATCCTCCAAA ATGAAAAGAA GAAAATGCAA GCTCACATTC AGGACCTAGA GGAGCAACTG 
GATGAGGAAG AAGGCGCTCG CCAGAAACTA CAGCTGGAAA AGGTGACAGC GGAAGCAAAA 
ATCAAGAAGA TGGAGGAGGA GATCCTGCTG CTTGAGGACC AGAATTCCAA ATTTATCAAA 
GAAAAGAAAC TCATGGAAGA TCGCATTGCA GAGTGTTCCT CTCAGCTGGC TGAGGAGGAA 
GAAAAGGCCA AAAACTTGGC CAAAATCAGG AATAAGCAGG AAGTGATGAT CTCAGATTTA 
GAAGAACGCT TGAAGAAGGA AGAGAAGACG CGTCAGGAGC TGGAGAAGGC CAAACGAAAA 
CTGGACGGTG AGACCACTGA CCTGCAGGAC CAGATCGCAG AGCTCCAGGC GCAGATCGAC 
GAGCTCAAGG TCCAGCTGGC CAAGAAGGAG GAGGAGCTGC AAGGCGCGCT GGCCAGGGGT 
GACGATGAGA CGCTCCATAA GAACAATGCC CTCAAGGTGG TGCGAGAGCT GCAAGCCCAA 
ATCGCCGAGC TTCAGGAGGA CTTTGAATCA GAGAAGGCTT CACGGAACAA GGCGGAGAAG 
CAGAAGAGGG ACCTGAGTGA GGAGCTGGAG GCCCTGAAAA CAGAGCTGGA GGACACGCTG 
GACACCACCG CGGCTCAGCA GGAACTTCGC ACGAAGCGTG AACAAGAAGT GGCAGAGCTG 
AAGAAGGCTC TGGAGGAGGA AACGAGGAGC CACGAGGCTC AAATCCAGGA CATGAGACAG 
CGACACGCCA CAGCCCTGGA GGAGCTGTCG GAGCAGCTGG AACAGGCAAA GAGGTTCAAA 
GCGAATCTGG AGAAGAACAA GCAGGGCCTG GAGACTGACA ACAAGGAGCT GGCGTGTGAG 
GTGAAGGTGC TACAGCAGGT CAAGGCTGAG TCTGAGCACA AGAGGAAGAA GCTCGACGCC 
CAGGTGCAGG AGCTGCACGC CAAGGTCTCA GAAGGAGACA GGCTCAGGGT GGAGCTGGCC 
GAGAAGGCAA ACAAATTACA GAATGAACTG GACAATGTCT CAAGTCTTCT GGAAGAAGCA 
GAGAAGAAGG GCATTAAGTT TGCTAAGGAC GCAGCTAGTC TTGAGTCTCA ACTACAGGAC 
ACTCAGGAGC TCCTTCAGGA GGAGACCCGC CAGAAGCTGA ACCTGAGCAG CCGCATCCGG 
CAGCTGGAGG AGGAGAAGAA CAGTCTCCAG GAGCAGCAGG AGGAGGAGGA GGAGGCCAGG 
AAAAACCTGG AAAAGCAGGT GTTGGTGCTG CAGTCCCAGC TCGCCGATAC CAAGAAGAAG 
GTAGATGATG ACTTAGGAAC CATCGAGAGC TTAGAAGAAG CCAGAAAGAA GCTTCTGAAA 
GATGTGGAGG CCCTGGGCCA GCGCCTGGAG GAGAAGGCTC TGGCCTATGA CAAGCTGGAG 
AAGACCAAGA ACCGCCTGCA GCAGGAGCTC GACGACCTGA CAGTGGACCT AGATCACCAG 
CGCCAGATCG CCTCCAACTT GGAGAAGAAG CAGAAGAAGT TTGACCAGCT GCTGGCAGAG 
GAGAAGAACA TCTCCGCCCG CTATGCAGAA GAGCGAGACC GCGCAGAGGC AGAGGCCAGG 
GAGAAGGAAA CCAAAGCCCT GTCCCTGGCC CGAGCCCTCG AGGAGGCCCT AGAAACCAAG 
GAAGAGTTTG AGAGACAGAA CAAGCAGCTG CGGGCAGACA TGGAGGACCT GATAAGCTCC 
AAGGATGACG TGGGGAAGAA CGTGCACGAG CTTGAAAAAT CGAAGCGGGC CCTGGAGCAA 
CAGGTGGAGG AGATGAGGAC ACAGCTGGAA GAGCTGGAGG ATGAGCTTCA GGCCACAGAA 
GACGCCAAGC TTCGCCTGGA AGTCAACATG CAGGCCATGA AGGCCCAGTT TGAGAGGGAC 
TTACAAACCA GGGACGAGCA GAATGAAGAG AAGAAGCGGC TCCTGGTTAA GCAGGTGCGG 
GAGCTGGAGG CAGAGCTGGA AGATGAGCGG AAGCAGCGAG CGCTGGCGGT CGCTTCTAAA 
AAGAAACTGG AAATAGACCT GAAGGACCTT GAAGCCCAGA TTGAGGCTGC GAACAAAGCG 
AGGGACGAAG TGATCAAGCA GCTTCGCAAG CTTCAGGCTC AGATGAAGGA TTACCAACGT 
GAATTAGAAG AAGCCCGTGC ATCCCGGGAT GAGATTTTTG CTCAATCCAA AGAGAGTGAA 
AAGAAACTGA AGAGTCTGGA AGCAGAAATC CTTCAATTGC AGGAGGAACT GGCCTCATCT 
GAGCGAGCGC GCCGCCACGC CGAGCAGGAG AGAGATGAGC TGGCTGACGA GATCGCCAAC 
AGCGCCTCTG GCAAGTCTGC GCTGCTGGAT GAGAAGCGGC GTCTGGAGGC ACGGATCGCG 
CAGCTGGAGG AGGAGCTGGA AGAGGAGCAG AGCAACATGG AGCTGCTCAA CGACCGCTTC 
CGCAAGACCA CGCTGCAGGT GGACACGCTG AACGCCGAGC TCGCGGCTGA GCGCAGCGCC 
GCCCAGAAGA GCGACAACGC ACGCCAGCAG CTAGAGCGGC AGAACAAGGA GCTGAAGGCC 
AAGCTGCAGG AACTGGAGGG CGCCGTCAAG TCCAAGTTCA AGGCCACCAT CTCTGCCCTG 
GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG CTGGAGCAGG AAGCCAAGGA ACGAGCAGCC 
GCCAACAAAC TAGTCCGTCG CACGGAGAAA AAGCTGAAAG AGATCTTCAT GCAGGTTGAA 
GATGAGCGTC GACATGCGGA TCAGTATAAA GAACAGATGG AGAAGGCCAA CGCCAGGATG 
AAACAGCTCA AGCGGCAGCT GGAGGAGGCA GAGGAGGAAG CCACACGTGC CAACGCATCT 
CGGCGTAAGC TCCAGCGGGA ACTGGACGAT GCCACGGAGG CCAACGAGGG CCTGAGCCGT 
GAGGTCAGCA CGCTGAAGAA CCGGCTGAGA CGAGGTGGCC CCATAAGCTT CTCGTCCAGC 
CGATCGGGCC GGCGCCAGCT ACACATCGAG GGAGCCTCCC TGGAGCTGTC AGACGATGAC 
ACGGAGAGTA AGACCAGTGA CGTCAACGAG ACACAGCCGC CGCAGTCGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_014113597.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969072.1 Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X4, mRNA.

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CloneID	OCe36459
Clone ID Related Accession (Same CDS sequence)	XM_014113597.2 , XM_014113597.1
Accession Version	XM_014113597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	myosin-10 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113597.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTCAGGAAT CGCCTAAACC AGTGAAACAT 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC CTTTGGAAAT 
GCGAAGACTG TGAAAAATGA TAATTCATCT CGCTTTGGCA AGTTTATTCG AATCAACTTT 
GATGTAACTG GCTATATTGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAGTT GTTATCTGGA 
GCAGGAGAAC ACCTAAAGTC GGATTTGCTC CTCGAAGGAT TTAATAACTA CAGATTCCTC 
TCCAACGGCT ATATTCCCAT CCCGGGACAG CAAGACAAGG ACAACTTTCA GGAAACCATG 
GAAGCAATGC ACATCATGGG CTTCTCTCAC GAGGAGATTC TGTCAATGCT TAAAGTAGTG 
TCATCAGTGC TACAGTTCGG AAATATTTCT TTCAAAAAGG AGCGAAATAC TGATCAAGCA 
TCCATGCCAG AGAACACAGT TGCACAGAAA CTCTGCCATC TTCTTGGGAT GAACGTGATG 
GAGTTCACGC GGGCCATCCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA TGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCG GTAGAAGCAT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GTTGGCTCGT TCACCGTATC AACAAAGCTC TGGACAGGAC CAAGCGTCAG 
GGCGCATCTT TTATTGGAAT CCTGGATATC GCTGGATTCG AAATATTTGA GCTCAACTCG 
TTCGAGCAAC TGTGCATCAA CTACACCAAT GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAG CTTCATTGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATCGAC CTCATCGAGA GACCCGCTAA CCCCCCTGGT 
GTGCTGGCCC TTTTGGATGA AGAGTGCTGG TTCCCGAAGG CCACGGACAA AACCTTTGTT 
GAAAAACTAG TTCAAGAGCA AGGCTCTCAC TCCAAGTTCC AGAAACCCCG GCAACTGAAA 
GACAGAGCTG ACTTCTGCAT CATCCATTAT GCGGGCAAGG TGGACTACAA GGCTGATGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAC GACAATGTGG CCACCCTCCT GCACCAGTCA 
TCTGACAGAT TCGTGGCAGA GCTTTGGAAG GATGTGGACC GTATCGTGGG TCTGGATCAG 
GTCACGGGCA TGACGGAGAC AGCCTTTGGC TCTGCGTATA AAACCAAGAA GGGCATGTTT 
CGGACTGTCG GGCAGCTCTA CAAAGAATCC CTCACCAAGC TGATGGCAAC TCTCCGGAAC 
ACCAACCCTA ACTTCGTCCG CTGCATCATT CCAAATCACG AGAAGCGAGC TGGAAAACTG 
GATCCGCACC TAGTGCTTGA TCAGCTTCGC TGTAACGGCG TTCTGGAAGG AATCAGGATC 
TGTCGCCAGG GGTTCCCCAA CCGGATAGTT TTCCAGGAAT TCAGACAGAG ATACGAGATC 
CTAACTCCAA ATGCTATCCC TAAGGGTTTC ATGGACGGCA AACAGGCCTG CGAACGAATG 
ATCCGGGCTT TAGAACTGGA CCCAAACTTG TACAGAATCG GACAGAGCAA GATATTTTTC 
CGAGCTGGCG TGCTGGCACA CTTAGAGGAA GAGAGGGATC TAAAGATCAC CGACATCATT 
ATCTTCTTCC AGGCTGTCTG CAGAGGCTAC CTGGCCAGAA AGGCCTTCGC CAAGAAGCAG 
CAGCAGCTGA GCGCCCTGAA GGTCCTGCAG CGGAACTGCG CCGCATACCT GAAGCTCCGG 
CACTGGCAGT GGTGGCGTGT CTTCACGAAG GTGAAGCCGC TCCTGCAAGT GACCCGCCAG 
GAGGAAGAGC TTCAGGCCAA AGACGAGGAG CTGCTGAAGG TGAAGGAGAG GCAGACGAAG 
GTGGAGGGCG AGCTGGAGGA AATGGAGAGG AAGCACCAGC AGCTCCTGGA AGAGAAGAAC 
ATCCTAACAG AGCAGCTCCA AGCAGAGACA GAGCTCTTTG CTGAGGCAGA AGAGATGAGA 
GCCAGGCTCG CCGCCAAGAA GCAGGAGCTG GAGGAGATTC TCCATGACTT GGAATCCAGG 
GTCGAAGAGG AAGAAGAACG AAACCAAATC CTCCAAAATG AAAAGAAGAA AATGCAAGCT 
CACATTCAGG ACCTAGAGGA GCAACTGGAT GAGGAAGAAG GCGCTCGCCA GAAACTACAG 
CTGGAAAAGG TGACAGCGGA AGCAAAAATC AAGAAGATGG AGGAGGAGAT CCTGCTGCTT 
GAGGACCAGA ATTCCAAATT TATCAAAGAA AAGAAACTCA TGGAAGATCG CATTGCAGAG 
TGTTCCTCTC AGCTGGCTGA GGAGGAAGAA AAGGCCAAAA ACTTGGCCAA AATCAGGAAT 
AAGCAGGAAG TGATGATCTC AGATTTAGAA GAACGCTTGA AGAAGGAAGA GAAGACGCGT 
CAGGAGCTGG AGAAGGCCAA ACGAAAACTG GACGGTGAGA CCACTGACCT GCAGGACCAG 
ATCGCAGAGC TCCAGGCGCA GATCGACGAG CTCAAGGTCC AGCTGGCCAA GAAGGAGGAG 
GAGCTGCAAG GCGCGCTGGC CAGGGGTGAC GATGAGACGC TCCATAAGAA CAATGCCCTC 
AAGGTGGTGC GAGAGCTGCA AGCCCAAATC GCCGAGCTTC AGGAGGACTT TGAATCAGAG 
AAGGCTTCAC GGAACAAGGC GGAGAAGCAG AAGAGGGACC TGAGTGAGGA GCTGGAGGCC 
CTGAAAACAG AGCTGGAGGA CACGCTGGAC ACCACCGCGG CTCAGCAGGA ACTTCGCACG 
AAGCGTGAAC AAGAAGTGGC AGAGCTGAAG AAGGCTCTGG AGGAGGAAAC GAGGAGCCAC 
GAGGCTCAAA TCCAGGACAT GAGACAGCGA CACGCCACAG CCCTGGAGGA GCTGTCGGAG 
CAGCTGGAAC AGGCAAAGAG GTTCAAAGCG AATCTGGAGA AGAACAAGCA GGGCCTGGAG 
ACTGACAACA AGGAGCTGGC GTGTGAGGTG AAGGTGCTAC AGCAGGTCAA GGCTGAGTCT 
GAGCACAAGA GGAAGAAGCT CGACGCCCAG GTGCAGGAGC TGCACGCCAA GGTCTCAGAA 
GGAGACAGGC TCAGGGTGGA GCTGGCCGAG AAGGCAAACA AATTACAGAA TGAACTGGAC 
AATGTCTCAA GTCTTCTGGA AGAAGCAGAG AAGAAGGGCA TTAAGTTTGC TAAGGACGCA 
GCTAGTCTTG AGTCTCAACT ACAGGACACT CAGGAGCTCC TTCAGGAGGA GACCCGCCAG 
AAGCTGAACC TGAGCAGCCG CATCCGGCAG CTGGAGGAGG AGAAGAACAG TCTCCAGGAG 
CAGCAGGAGG AGGAGGAGGA GGCCAGGAAA AACCTGGAAA AGCAGGTGTT GGTGCTGCAG 
TCCCAGCTCG CCGATACCAA GAAGAAGGTA GATGATGACT TAGGAACCAT CGAGAGCTTA 
GAAGAAGCCA GAAAGAAGCT TCTGAAAGAT GTGGAGGCCC TGGGCCAGCG CCTGGAGGAG 
AAGGCTCTGG CCTATGACAA GCTGGAGAAG ACCAAGAACC GCCTGCAGCA GGAGCTCGAC 
GACCTGACAG TGGACCTAGA TCACCAGCGC CAGATCGCCT CCAACTTGGA GAAGAAGCAG 
AAGAAGTTTG ACCAGCTGCT GGCAGAGGAG AAGAACATCT CCGCCCGCTA TGCAGAAGAG 
CGAGACCGCG CAGAGGCAGA GGCCAGGGAG AAGGAAACCA AAGCCCTGTC CCTGGCCCGA 
GCCCTCGAGG AGGCCCTAGA AACCAAGGAA GAGTTTGAGA GACAGAACAA GCAGCTGCGG 
GCAGACATGG AGGACCTGAT AAGCTCCAAG GATGACGTGG GGAAGAACGT GCACGAGCTT 
GAAAAATCGA AGCGGGCCCT GGAGCAACAG GTGGAGGAGA TGAGGACACA GCTGGAAGAG 
CTGGAGGATG AGCTTCAGGC CACAGAAGAC GCCAAGCTTC GCCTGGAAGT CAACATGCAG 
GCCATGAAGG CCCAGTTTGA GAGGGACTTA CAAACCAGGG ACGAGCAGAA TGAAGAGAAG 
AAGCGGCTCC TGGTTAAGCA GGTGCGGGAG CTGGAGGCAG AGCTGGAAGA TGAGCGGAAG 
CAGCGAGCGC TGGCGGTCGC TTCTAAAAAG AAACTGGAAA TAGACCTGAA GGACCTTGAA 
GCCCAGATTG AGGCTGCGAA CAAAGCGAGG GACGAAGTGA TCAAGCAGCT TCGCAAGCTT 
CAGGCTCAGA TGAAGGATTA CCAACGTGAA TTAGAAGAAG CCCGTGCATC CCGGGATGAG 
ATTTTTGCTC AATCCAAAGA GAGTGAAAAG AAACTGAAGA GTCTGGAAGC AGAAATCCTT 
CAATTGCAGG AGGAACTGGC CTCATCTGAG CGAGCGCGCC GCCACGCCGA GCAGGAGAGA 
GATGAGCTGG CTGACGAGAT CGCCAACAGC GCCTCTGGCA AGTCTGCGCT GCTGGATGAG 
AAGCGGCGTC TGGAGGCACG GATCGCGCAG CTGGAGGAGG AGCTGGAAGA GGAGCAGAGC 
AACATGGAGC TGCTCAACGA CCGCTTCCGC AAGACCACGC TGCAGGTGGA CACGCTGAAC 
GCCGAGCTCG CGGCTGAGCG CAGCGCCGCC CAGAAGAGCG ACAACGCACG CCAGCAGCTA 
GAGCGGCAGA ACAAGGAGCT GAAGGCCAAG CTGCAGGAAC TGGAGGGCGC CGTCAAGTCC 
AAGTTCAAGG CCACCATCTC TGCCCTGGAA GCCAAGATTG GGCAGCTGGA GGAGCAGCTG 
GAGCAGGAAG CCAAGGAACG AGCAGCCGCC AACAAACTAG TCCGTCGCAC GGAGAAAAAG 
CTGAAAGAGA TCTTCATGCA GGTTGAAGAT GAGCGTCGAC ATGCGGATCA GTATAAAGAA 
CAGATGGAGA AGGCCAACGC CAGGATGAAA CAGCTCAAGC GGCAGCTGGA GGAGGCAGAG 
GAGGAAGCCA CACGTGCCAA CGCATCTCGG CGTAAGCTCC AGCGGGAACT GGACGATGCC 
ACGGAGGCCA ACGAGGGCCT GAGCCGTGAG GTCAGCACGC TGAAGAACCG GCTGAGACGA 
GGTGGCCCCA TAAGCTTCTC GTCCAGCCGA TCGGGCCGGC GCCAGCTACA CATCGAGGGA 
GCCTCCCTGG AGCTGTCAGA CGATGACACG GAGAGTAAGA CCAGTGACGT CAACGAGACA 
CAGCCGCCGC AGTCGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969072.1
CDS	192..6152
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_014113597.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113597.2

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTCAGGAAT CGCCTAAACC AGTGAAACAT 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC CTTTGGAAAT 
GCGAAGACTG TGAAAAATGA TAATTCATCT CGCTTTGGCA AGTTTATTCG AATCAACTTT 
GATGTAACTG GCTATATTGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAGTT GTTATCTGGA 
GCAGGAGAAC ACCTAAAGTC GGATTTGCTC CTCGAAGGAT TTAATAACTA CAGATTCCTC 
TCCAACGGCT ATATTCCCAT CCCGGGACAG CAAGACAAGG ACAACTTTCA GGAAACCATG 
GAAGCAATGC ACATCATGGG CTTCTCTCAC GAGGAGATTC TGTCAATGCT TAAAGTAGTG 
TCATCAGTGC TACAGTTCGG AAATATTTCT TTCAAAAAGG AGCGAAATAC TGATCAAGCA 
TCCATGCCAG AGAACACAGT TGCACAGAAA CTCTGCCATC TTCTTGGGAT GAACGTGATG 
GAGTTCACGC GGGCCATCCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA TGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCG GTAGAAGCAT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GTTGGCTCGT TCACCGTATC AACAAAGCTC TGGACAGGAC CAAGCGTCAG 
GGCGCATCTT TTATTGGAAT CCTGGATATC GCTGGATTCG AAATATTTGA GCTCAACTCG 
TTCGAGCAAC TGTGCATCAA CTACACCAAT GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAG CTTCATTGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATCGAC CTCATCGAGA GACCCGCTAA CCCCCCTGGT 
GTGCTGGCCC TTTTGGATGA AGAGTGCTGG TTCCCGAAGG CCACGGACAA AACCTTTGTT 
GAAAAACTAG TTCAAGAGCA AGGCTCTCAC TCCAAGTTCC AGAAACCCCG GCAACTGAAA 
GACAGAGCTG ACTTCTGCAT CATCCATTAT GCGGGCAAGG TGGACTACAA GGCTGATGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAC GACAATGTGG CCACCCTCCT GCACCAGTCA 
TCTGACAGAT TCGTGGCAGA GCTTTGGAAG GATGTGGACC GTATCGTGGG TCTGGATCAG 
GTCACGGGCA TGACGGAGAC AGCCTTTGGC TCTGCGTATA AAACCAAGAA GGGCATGTTT 
CGGACTGTCG GGCAGCTCTA CAAAGAATCC CTCACCAAGC TGATGGCAAC TCTCCGGAAC 
ACCAACCCTA ACTTCGTCCG CTGCATCATT CCAAATCACG AGAAGCGAGC TGGAAAACTG 
GATCCGCACC TAGTGCTTGA TCAGCTTCGC TGTAACGGCG TTCTGGAAGG AATCAGGATC 
TGTCGCCAGG GGTTCCCCAA CCGGATAGTT TTCCAGGAAT TCAGACAGAG ATACGAGATC 
CTAACTCCAA ATGCTATCCC TAAGGGTTTC ATGGACGGCA AACAGGCCTG CGAACGAATG 
ATCCGGGCTT TAGAACTGGA CCCAAACTTG TACAGAATCG GACAGAGCAA GATATTTTTC 
CGAGCTGGCG TGCTGGCACA CTTAGAGGAA GAGAGGGATC TAAAGATCAC CGACATCATT 
ATCTTCTTCC AGGCTGTCTG CAGAGGCTAC CTGGCCAGAA AGGCCTTCGC CAAGAAGCAG 
CAGCAGCTGA GCGCCCTGAA GGTCCTGCAG CGGAACTGCG CCGCATACCT GAAGCTCCGG 
CACTGGCAGT GGTGGCGTGT CTTCACGAAG GTGAAGCCGC TCCTGCAAGT GACCCGCCAG 
GAGGAAGAGC TTCAGGCCAA AGACGAGGAG CTGCTGAAGG TGAAGGAGAG GCAGACGAAG 
GTGGAGGGCG AGCTGGAGGA AATGGAGAGG AAGCACCAGC AGCTCCTGGA AGAGAAGAAC 
ATCCTAACAG AGCAGCTCCA AGCAGAGACA GAGCTCTTTG CTGAGGCAGA AGAGATGAGA 
GCCAGGCTCG CCGCCAAGAA GCAGGAGCTG GAGGAGATTC TCCATGACTT GGAATCCAGG 
GTCGAAGAGG AAGAAGAACG AAACCAAATC CTCCAAAATG AAAAGAAGAA AATGCAAGCT 
CACATTCAGG ACCTAGAGGA GCAACTGGAT GAGGAAGAAG GCGCTCGCCA GAAACTACAG 
CTGGAAAAGG TGACAGCGGA AGCAAAAATC AAGAAGATGG AGGAGGAGAT CCTGCTGCTT 
GAGGACCAGA ATTCCAAATT TATCAAAGAA AAGAAACTCA TGGAAGATCG CATTGCAGAG 
TGTTCCTCTC AGCTGGCTGA GGAGGAAGAA AAGGCCAAAA ACTTGGCCAA AATCAGGAAT 
AAGCAGGAAG TGATGATCTC AGATTTAGAA GAACGCTTGA AGAAGGAAGA GAAGACGCGT 
CAGGAGCTGG AGAAGGCCAA ACGAAAACTG GACGGTGAGA CCACTGACCT GCAGGACCAG 
ATCGCAGAGC TCCAGGCGCA GATCGACGAG CTCAAGGTCC AGCTGGCCAA GAAGGAGGAG 
GAGCTGCAAG GCGCGCTGGC CAGGGGTGAC GATGAGACGC TCCATAAGAA CAATGCCCTC 
AAGGTGGTGC GAGAGCTGCA AGCCCAAATC GCCGAGCTTC AGGAGGACTT TGAATCAGAG 
AAGGCTTCAC GGAACAAGGC GGAGAAGCAG AAGAGGGACC TGAGTGAGGA GCTGGAGGCC 
CTGAAAACAG AGCTGGAGGA CACGCTGGAC ACCACCGCGG CTCAGCAGGA ACTTCGCACG 
AAGCGTGAAC AAGAAGTGGC AGAGCTGAAG AAGGCTCTGG AGGAGGAAAC GAGGAGCCAC 
GAGGCTCAAA TCCAGGACAT GAGACAGCGA CACGCCACAG CCCTGGAGGA GCTGTCGGAG 
CAGCTGGAAC AGGCAAAGAG GTTCAAAGCG AATCTGGAGA AGAACAAGCA GGGCCTGGAG 
ACTGACAACA AGGAGCTGGC GTGTGAGGTG AAGGTGCTAC AGCAGGTCAA GGCTGAGTCT 
GAGCACAAGA GGAAGAAGCT CGACGCCCAG GTGCAGGAGC TGCACGCCAA GGTCTCAGAA 
GGAGACAGGC TCAGGGTGGA GCTGGCCGAG AAGGCAAACA AATTACAGAA TGAACTGGAC 
AATGTCTCAA GTCTTCTGGA AGAAGCAGAG AAGAAGGGCA TTAAGTTTGC TAAGGACGCA 
GCTAGTCTTG AGTCTCAACT ACAGGACACT CAGGAGCTCC TTCAGGAGGA GACCCGCCAG 
AAGCTGAACC TGAGCAGCCG CATCCGGCAG CTGGAGGAGG AGAAGAACAG TCTCCAGGAG 
CAGCAGGAGG AGGAGGAGGA GGCCAGGAAA AACCTGGAAA AGCAGGTGTT GGTGCTGCAG 
TCCCAGCTCG CCGATACCAA GAAGAAGGTA GATGATGACT TAGGAACCAT CGAGAGCTTA 
GAAGAAGCCA GAAAGAAGCT TCTGAAAGAT GTGGAGGCCC TGGGCCAGCG CCTGGAGGAG 
AAGGCTCTGG CCTATGACAA GCTGGAGAAG ACCAAGAACC GCCTGCAGCA GGAGCTCGAC 
GACCTGACAG TGGACCTAGA TCACCAGCGC CAGATCGCCT CCAACTTGGA GAAGAAGCAG 
AAGAAGTTTG ACCAGCTGCT GGCAGAGGAG AAGAACATCT CCGCCCGCTA TGCAGAAGAG 
CGAGACCGCG CAGAGGCAGA GGCCAGGGAG AAGGAAACCA AAGCCCTGTC CCTGGCCCGA 
GCCCTCGAGG AGGCCCTAGA AACCAAGGAA GAGTTTGAGA GACAGAACAA GCAGCTGCGG 
GCAGACATGG AGGACCTGAT AAGCTCCAAG GATGACGTGG GGAAGAACGT GCACGAGCTT 
GAAAAATCGA AGCGGGCCCT GGAGCAACAG GTGGAGGAGA TGAGGACACA GCTGGAAGAG 
CTGGAGGATG AGCTTCAGGC CACAGAAGAC GCCAAGCTTC GCCTGGAAGT CAACATGCAG 
GCCATGAAGG CCCAGTTTGA GAGGGACTTA CAAACCAGGG ACGAGCAGAA TGAAGAGAAG 
AAGCGGCTCC TGGTTAAGCA GGTGCGGGAG CTGGAGGCAG AGCTGGAAGA TGAGCGGAAG 
CAGCGAGCGC TGGCGGTCGC TTCTAAAAAG AAACTGGAAA TAGACCTGAA GGACCTTGAA 
GCCCAGATTG AGGCTGCGAA CAAAGCGAGG GACGAAGTGA TCAAGCAGCT TCGCAAGCTT 
CAGGCTCAGA TGAAGGATTA CCAACGTGAA TTAGAAGAAG CCCGTGCATC CCGGGATGAG 
ATTTTTGCTC AATCCAAAGA GAGTGAAAAG AAACTGAAGA GTCTGGAAGC AGAAATCCTT 
CAATTGCAGG AGGAACTGGC CTCATCTGAG CGAGCGCGCC GCCACGCCGA GCAGGAGAGA 
GATGAGCTGG CTGACGAGAT CGCCAACAGC GCCTCTGGCA AGTCTGCGCT GCTGGATGAG 
AAGCGGCGTC TGGAGGCACG GATCGCGCAG CTGGAGGAGG AGCTGGAAGA GGAGCAGAGC 
AACATGGAGC TGCTCAACGA CCGCTTCCGC AAGACCACGC TGCAGGTGGA CACGCTGAAC 
GCCGAGCTCG CGGCTGAGCG CAGCGCCGCC CAGAAGAGCG ACAACGCACG CCAGCAGCTA 
GAGCGGCAGA ACAAGGAGCT GAAGGCCAAG CTGCAGGAAC TGGAGGGCGC CGTCAAGTCC 
AAGTTCAAGG CCACCATCTC TGCCCTGGAA GCCAAGATTG GGCAGCTGGA GGAGCAGCTG 
GAGCAGGAAG CCAAGGAACG AGCAGCCGCC AACAAACTAG TCCGTCGCAC GGAGAAAAAG 
CTGAAAGAGA TCTTCATGCA GGTTGAAGAT GAGCGTCGAC ATGCGGATCA GTATAAAGAA 
CAGATGGAGA AGGCCAACGC CAGGATGAAA CAGCTCAAGC GGCAGCTGGA GGAGGCAGAG 
GAGGAAGCCA CACGTGCCAA CGCATCTCGG CGTAAGCTCC AGCGGGAACT GGACGATGCC 
ACGGAGGCCA ACGAGGGCCT GAGCCGTGAG GTCAGCACGC TGAAGAACCG GCTGAGACGA 
GGTGGCCCCA TAAGCTTCTC GTCCAGCCGA TCGGGCCGGC GCCAGCTACA CATCGAGGGA 
GCCTCCCTGG AGCTGTCAGA CGATGACACG GAGAGTAAGA CCAGTGACGT CAACGAGACA 
CAGCCGCCGC AGTCGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_014113597.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969072.1 Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X4, mRNA.

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CloneID	OCe36459
Clone ID Related Accession (Same CDS sequence)	XM_014113597.2 , XM_014113597.1
Accession Version	XM_014113597.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	myosin-10 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTCAGGAAT CGCCTAAACC AGTGAAACAT 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC CTTTGGAAAT 
GCGAAGACTG TGAAAAATGA TAATTCATCT CGCTTTGGCA AGTTTATTCG AATCAACTTT 
GATGTAACTG GCTATATTGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAGTT GTTATCTGGA 
GCAGGAGAAC ACCTAAAGTC GGATTTGCTC CTCGAAGGAT TTAATAACTA CAGATTCCTC 
TCCAACGGCT ATATTCCCAT CCCGGGACAG CAAGACAAGG ACAACTTTCA GGAAACCATG 
GAAGCAATGC ACATCATGGG CTTCTCTCAC GAGGAGATTC TGTCAATGCT TAAAGTAGTG 
TCATCAGTGC TACAGTTCGG AAATATTTCT TTCAAAAAGG AGCGAAATAC TGATCAAGCA 
TCCATGCCAG AGAACACAGT TGCACAGAAA CTCTGCCATC TTCTTGGGAT GAACGTGATG 
GAGTTCACGC GGGCCATCCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA TGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCG GTAGAAGCAT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GTTGGCTCGT TCACCGTATC AACAAAGCTC TGGACAGGAC CAAGCGTCAG 
GGCGCATCTT TTATTGGAAT CCTGGATATC GCTGGATTCG AAATATTTGA GCTCAACTCG 
TTCGAGCAAC TGTGCATCAA CTACACCAAT GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAG CTTCATTGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATCGAC CTCATCGAGA GACCCGCTAA CCCCCCTGGT 
GTGCTGGCCC TTTTGGATGA AGAGTGCTGG TTCCCGAAGG CCACGGACAA AACCTTTGTT 
GAAAAACTAG TTCAAGAGCA AGGCTCTCAC TCCAAGTTCC AGAAACCCCG GCAACTGAAA 
GACAGAGCTG ACTTCTGCAT CATCCATTAT GCGGGCAAGG TGGACTACAA GGCTGATGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAC GACAATGTGG CCACCCTCCT GCACCAGTCA 
TCTGACAGAT TCGTGGCAGA GCTTTGGAAG GATGTGGACC GTATCGTGGG TCTGGATCAG 
GTCACGGGCA TGACGGAGAC AGCCTTTGGC TCTGCGTATA AAACCAAGAA GGGCATGTTT 
CGGACTGTCG GGCAGCTCTA CAAAGAATCC CTCACCAAGC TGATGGCAAC TCTCCGGAAC 
ACCAACCCTA ACTTCGTCCG CTGCATCATT CCAAATCACG AGAAGCGAGC TGGAAAACTG 
GATCCGCACC TAGTGCTTGA TCAGCTTCGC TGTAACGGCG TTCTGGAAGG AATCAGGATC 
TGTCGCCAGG GGTTCCCCAA CCGGATAGTT TTCCAGGAAT TCAGACAGAG ATACGAGATC 
CTAACTCCAA ATGCTATCCC TAAGGGTTTC ATGGACGGCA AACAGGCCTG CGAACGAATG 
ATCCGGGCTT TAGAACTGGA CCCAAACTTG TACAGAATCG GACAGAGCAA GATATTTTTC 
CGAGCTGGCG TGCTGGCACA CTTAGAGGAA GAGAGGGATC TAAAGATCAC CGACATCATT 
ATCTTCTTCC AGGCTGTCTG CAGAGGCTAC CTGGCCAGAA AGGCCTTCGC CAAGAAGCAG 
CAGCAGCTGA GCGCCCTGAA GGTCCTGCAG CGGAACTGCG CCGCATACCT GAAGCTCCGG 
CACTGGCAGT GGTGGCGTGT CTTCACGAAG GTGAAGCCGC TCCTGCAAGT GACCCGCCAG 
GAGGAAGAGC TTCAGGCCAA AGACGAGGAG CTGCTGAAGG TGAAGGAGAG GCAGACGAAG 
GTGGAGGGCG AGCTGGAGGA AATGGAGAGG AAGCACCAGC AGCTCCTGGA AGAGAAGAAC 
ATCCTAACAG AGCAGCTCCA AGCAGAGACA GAGCTCTTTG CTGAGGCAGA AGAGATGAGA 
GCCAGGCTCG CCGCCAAGAA GCAGGAGCTG GAGGAGATTC TCCATGACTT GGAATCCAGG 
GTCGAAGAGG AAGAAGAACG AAACCAAATC CTCCAAAATG AAAAGAAGAA AATGCAAGCT 
CACATTCAGG ACCTAGAGGA GCAACTGGAT GAGGAAGAAG GCGCTCGCCA GAAACTACAG 
CTGGAAAAGG TGACAGCGGA AGCAAAAATC AAGAAGATGG AGGAGGAGAT CCTGCTGCTT 
GAGGACCAGA ATTCCAAATT TATCAAAGAA AAGAAACTCA TGGAAGATCG CATTGCAGAG 
TGTTCCTCTC AGCTGGCTGA GGAGGAAGAA AAGGCCAAAA ACTTGGCCAA AATCAGGAAT 
AAGCAGGAAG TGATGATCTC AGATTTAGAA GAACGCTTGA AGAAGGAAGA GAAGACGCGT 
CAGGAGCTGG AGAAGGCCAA ACGAAAACTG GACGGTGAGA CCACTGACCT GCAGGACCAG 
ATCGCAGAGC TCCAGGCGCA GATCGACGAG CTCAAGGTCC AGCTGGCCAA GAAGGAGGAG 
GAGCTGCAAG GCGCGCTGGC CAGGGGTGAC GATGAGACGC TCCATAAGAA CAATGCCCTC 
AAGGTGGTGC GAGAGCTGCA AGCCCAAATC GCCGAGCTTC AGGAGGACTT TGAATCAGAG 
AAGGCTTCAC GGAACAAGGC GGAGAAGCAG AAGAGGGACC TGAGTGAGGA GCTGGAGGCC 
CTGAAAACAG AGCTGGAGGA CACGCTGGAC ACCACCGCGG CTCAGCAGGA ACTTCGCACG 
AAGCGTGAAC AAGAAGTGGC AGAGCTGAAG AAGGCTCTGG AGGAGGAAAC GAGGAGCCAC 
GAGGCTCAAA TCCAGGACAT GAGACAGCGA CACGCCACAG CCCTGGAGGA GCTGTCGGAG 
CAGCTGGAAC AGGCAAAGAG GTTCAAAGCG AATCTGGAGA AGAACAAGCA GGGCCTGGAG 
ACTGACAACA AGGAGCTGGC GTGTGAGGTG AAGGTGCTAC AGCAGGTCAA GGCTGAGTCT 
GAGCACAAGA GGAAGAAGCT CGACGCCCAG GTGCAGGAGC TGCACGCCAA GGTCTCAGAA 
GGAGACAGGC TCAGGGTGGA GCTGGCCGAG AAGGCAAACA AATTACAGAA TGAACTGGAC 
AATGTCTCAA GTCTTCTGGA AGAAGCAGAG AAGAAGGGCA TTAAGTTTGC TAAGGACGCA 
GCTAGTCTTG AGTCTCAACT ACAGGACACT CAGGAGCTCC TTCAGGAGGA GACCCGCCAG 
AAGCTGAACC TGAGCAGCCG CATCCGGCAG CTGGAGGAGG AGAAGAACAG TCTCCAGGAG 
CAGCAGGAGG AGGAGGAGGA GGCCAGGAAA AACCTGGAAA AGCAGGTGTT GGTGCTGCAG 
TCCCAGCTCG CCGATACCAA GAAGAAGGTA GATGATGACT TAGGAACCAT CGAGAGCTTA 
GAAGAAGCCA GAAAGAAGCT TCTGAAAGAT GTGGAGGCCC TGGGCCAGCG CCTGGAGGAG 
AAGGCTCTGG CCTATGACAA GCTGGAGAAG ACCAAGAACC GCCTGCAGCA GGAGCTCGAC 
GACCTGACAG TGGACCTAGA TCACCAGCGC CAGATCGCCT CCAACTTGGA GAAGAAGCAG 
AAGAAGTTTG ACCAGCTGCT GGCAGAGGAG AAGAACATCT CCGCCCGCTA TGCAGAAGAG 
CGAGACCGCG CAGAGGCAGA GGCCAGGGAG AAGGAAACCA AAGCCCTGTC CCTGGCCCGA 
GCCCTCGAGG AGGCCCTAGA AACCAAGGAA GAGTTTGAGA GACAGAACAA GCAGCTGCGG 
GCAGACATGG AGGACCTGAT AAGCTCCAAG GATGACGTGG GGAAGAACGT GCACGAGCTT 
GAAAAATCGA AGCGGGCCCT GGAGCAACAG GTGGAGGAGA TGAGGACACA GCTGGAAGAG 
CTGGAGGATG AGCTTCAGGC CACAGAAGAC GCCAAGCTTC GCCTGGAAGT CAACATGCAG 
GCCATGAAGG CCCAGTTTGA GAGGGACTTA CAAACCAGGG ACGAGCAGAA TGAAGAGAAG 
AAGCGGCTCC TGGTTAAGCA GGTGCGGGAG CTGGAGGCAG AGCTGGAAGA TGAGCGGAAG 
CAGCGAGCGC TGGCGGTCGC TTCTAAAAAG AAACTGGAAA TAGACCTGAA GGACCTTGAA 
GCCCAGATTG AGGCTGCGAA CAAAGCGAGG GACGAAGTGA TCAAGCAGCT TCGCAAGCTT 
CAGGCTCAGA TGAAGGATTA CCAACGTGAA TTAGAAGAAG CCCGTGCATC CCGGGATGAG 
ATTTTTGCTC AATCCAAAGA GAGTGAAAAG AAACTGAAGA GTCTGGAAGC AGAAATCCTT 
CAATTGCAGG AGGAACTGGC CTCATCTGAG CGAGCGCGCC GCCACGCCGA GCAGGAGAGA 
GATGAGCTGG CTGACGAGAT CGCCAACAGC GCCTCTGGCA AGTCTGCGCT GCTGGATGAG 
AAGCGGCGTC TGGAGGCACG GATCGCGCAG CTGGAGGAGG AGCTGGAAGA GGAGCAGAGC 
AACATGGAGC TGCTCAACGA CCGCTTCCGC AAGACCACGC TGCAGGTGGA CACGCTGAAC 
GCCGAGCTCG CGGCTGAGCG CAGCGCCGCC CAGAAGAGCG ACAACGCACG CCAGCAGCTA 
GAGCGGCAGA ACAAGGAGCT GAAGGCCAAG CTGCAGGAAC TGGAGGGCGC CGTCAAGTCC 
AAGTTCAAGG CCACCATCTC TGCCCTGGAA GCCAAGATTG GGCAGCTGGA GGAGCAGCTG 
GAGCAGGAAG CCAAGGAACG AGCAGCCGCC AACAAACTAG TCCGTCGCAC GGAGAAAAAG 
CTGAAAGAGA TCTTCATGCA GGTTGAAGAT GAGCGTCGAC ATGCGGATCA GTATAAAGAA 
CAGATGGAGA AGGCCAACGC CAGGATGAAA CAGCTCAAGC GGCAGCTGGA GGAGGCAGAG 
GAGGAAGCCA CACGTGCCAA CGCATCTCGG CGTAAGCTCC AGCGGGAACT GGACGATGCC 
ACGGAGGCCA ACGAGGGCCT GAGCCGTGAG GTCAGCACGC TGAAGAACCG GCTGAGACGA 
GGTGGCCCCA TAAGCTTCTC GTCCAGCCGA TCGGGCCGGC GCCAGCTACA CATCGAGGGA 
GCCTCCCTGG AGCTGTCAGA CGATGACACG GAGAGTAAGA CCAGTGACGT CAACGAGACA 
CAGCCGCCGC AGTCGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969072.1
CDS	132..6092
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_014113597.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113597.1

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTCAGGAAT CGCCTAAACC AGTGAAACAT 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC CTTTGGAAAT 
GCGAAGACTG TGAAAAATGA TAATTCATCT CGCTTTGGCA AGTTTATTCG AATCAACTTT 
GATGTAACTG GCTATATTGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAGTT GTTATCTGGA 
GCAGGAGAAC ACCTAAAGTC GGATTTGCTC CTCGAAGGAT TTAATAACTA CAGATTCCTC 
TCCAACGGCT ATATTCCCAT CCCGGGACAG CAAGACAAGG ACAACTTTCA GGAAACCATG 
GAAGCAATGC ACATCATGGG CTTCTCTCAC GAGGAGATTC TGTCAATGCT TAAAGTAGTG 
TCATCAGTGC TACAGTTCGG AAATATTTCT TTCAAAAAGG AGCGAAATAC TGATCAAGCA 
TCCATGCCAG AGAACACAGT TGCACAGAAA CTCTGCCATC TTCTTGGGAT GAACGTGATG 
GAGTTCACGC GGGCCATCCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA TGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCG GTAGAAGCAT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GTTGGCTCGT TCACCGTATC AACAAAGCTC TGGACAGGAC CAAGCGTCAG 
GGCGCATCTT TTATTGGAAT CCTGGATATC GCTGGATTCG AAATATTTGA GCTCAACTCG 
TTCGAGCAAC TGTGCATCAA CTACACCAAT GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAG CTTCATTGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATCGAC CTCATCGAGA GACCCGCTAA CCCCCCTGGT 
GTGCTGGCCC TTTTGGATGA AGAGTGCTGG TTCCCGAAGG CCACGGACAA AACCTTTGTT 
GAAAAACTAG TTCAAGAGCA AGGCTCTCAC TCCAAGTTCC AGAAACCCCG GCAACTGAAA 
GACAGAGCTG ACTTCTGCAT CATCCATTAT GCGGGCAAGG TGGACTACAA GGCTGATGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAC GACAATGTGG CCACCCTCCT GCACCAGTCA 
TCTGACAGAT TCGTGGCAGA GCTTTGGAAG GATGTGGACC GTATCGTGGG TCTGGATCAG 
GTCACGGGCA TGACGGAGAC AGCCTTTGGC TCTGCGTATA AAACCAAGAA GGGCATGTTT 
CGGACTGTCG GGCAGCTCTA CAAAGAATCC CTCACCAAGC TGATGGCAAC TCTCCGGAAC 
ACCAACCCTA ACTTCGTCCG CTGCATCATT CCAAATCACG AGAAGCGAGC TGGAAAACTG 
GATCCGCACC TAGTGCTTGA TCAGCTTCGC TGTAACGGCG TTCTGGAAGG AATCAGGATC 
TGTCGCCAGG GGTTCCCCAA CCGGATAGTT TTCCAGGAAT TCAGACAGAG ATACGAGATC 
CTAACTCCAA ATGCTATCCC TAAGGGTTTC ATGGACGGCA AACAGGCCTG CGAACGAATG 
ATCCGGGCTT TAGAACTGGA CCCAAACTTG TACAGAATCG GACAGAGCAA GATATTTTTC 
CGAGCTGGCG TGCTGGCACA CTTAGAGGAA GAGAGGGATC TAAAGATCAC CGACATCATT 
ATCTTCTTCC AGGCTGTCTG CAGAGGCTAC CTGGCCAGAA AGGCCTTCGC CAAGAAGCAG 
CAGCAGCTGA GCGCCCTGAA GGTCCTGCAG CGGAACTGCG CCGCATACCT GAAGCTCCGG 
CACTGGCAGT GGTGGCGTGT CTTCACGAAG GTGAAGCCGC TCCTGCAAGT GACCCGCCAG 
GAGGAAGAGC TTCAGGCCAA AGACGAGGAG CTGCTGAAGG TGAAGGAGAG GCAGACGAAG 
GTGGAGGGCG AGCTGGAGGA AATGGAGAGG AAGCACCAGC AGCTCCTGGA AGAGAAGAAC 
ATCCTAACAG AGCAGCTCCA AGCAGAGACA GAGCTCTTTG CTGAGGCAGA AGAGATGAGA 
GCCAGGCTCG CCGCCAAGAA GCAGGAGCTG GAGGAGATTC TCCATGACTT GGAATCCAGG 
GTCGAAGAGG AAGAAGAACG AAACCAAATC CTCCAAAATG AAAAGAAGAA AATGCAAGCT 
CACATTCAGG ACCTAGAGGA GCAACTGGAT GAGGAAGAAG GCGCTCGCCA GAAACTACAG 
CTGGAAAAGG TGACAGCGGA AGCAAAAATC AAGAAGATGG AGGAGGAGAT CCTGCTGCTT 
GAGGACCAGA ATTCCAAATT TATCAAAGAA AAGAAACTCA TGGAAGATCG CATTGCAGAG 
TGTTCCTCTC AGCTGGCTGA GGAGGAAGAA AAGGCCAAAA ACTTGGCCAA AATCAGGAAT 
AAGCAGGAAG TGATGATCTC AGATTTAGAA GAACGCTTGA AGAAGGAAGA GAAGACGCGT 
CAGGAGCTGG AGAAGGCCAA ACGAAAACTG GACGGTGAGA CCACTGACCT GCAGGACCAG 
ATCGCAGAGC TCCAGGCGCA GATCGACGAG CTCAAGGTCC AGCTGGCCAA GAAGGAGGAG 
GAGCTGCAAG GCGCGCTGGC CAGGGGTGAC GATGAGACGC TCCATAAGAA CAATGCCCTC 
AAGGTGGTGC GAGAGCTGCA AGCCCAAATC GCCGAGCTTC AGGAGGACTT TGAATCAGAG 
AAGGCTTCAC GGAACAAGGC GGAGAAGCAG AAGAGGGACC TGAGTGAGGA GCTGGAGGCC 
CTGAAAACAG AGCTGGAGGA CACGCTGGAC ACCACCGCGG CTCAGCAGGA ACTTCGCACG 
AAGCGTGAAC AAGAAGTGGC AGAGCTGAAG AAGGCTCTGG AGGAGGAAAC GAGGAGCCAC 
GAGGCTCAAA TCCAGGACAT GAGACAGCGA CACGCCACAG CCCTGGAGGA GCTGTCGGAG 
CAGCTGGAAC AGGCAAAGAG GTTCAAAGCG AATCTGGAGA AGAACAAGCA GGGCCTGGAG 
ACTGACAACA AGGAGCTGGC GTGTGAGGTG AAGGTGCTAC AGCAGGTCAA GGCTGAGTCT 
GAGCACAAGA GGAAGAAGCT CGACGCCCAG GTGCAGGAGC TGCACGCCAA GGTCTCAGAA 
GGAGACAGGC TCAGGGTGGA GCTGGCCGAG AAGGCAAACA AATTACAGAA TGAACTGGAC 
AATGTCTCAA GTCTTCTGGA AGAAGCAGAG AAGAAGGGCA TTAAGTTTGC TAAGGACGCA 
GCTAGTCTTG AGTCTCAACT ACAGGACACT CAGGAGCTCC TTCAGGAGGA GACCCGCCAG 
AAGCTGAACC TGAGCAGCCG CATCCGGCAG CTGGAGGAGG AGAAGAACAG TCTCCAGGAG 
CAGCAGGAGG AGGAGGAGGA GGCCAGGAAA AACCTGGAAA AGCAGGTGTT GGTGCTGCAG 
TCCCAGCTCG CCGATACCAA GAAGAAGGTA GATGATGACT TAGGAACCAT CGAGAGCTTA 
GAAGAAGCCA GAAAGAAGCT TCTGAAAGAT GTGGAGGCCC TGGGCCAGCG CCTGGAGGAG 
AAGGCTCTGG CCTATGACAA GCTGGAGAAG ACCAAGAACC GCCTGCAGCA GGAGCTCGAC 
GACCTGACAG TGGACCTAGA TCACCAGCGC CAGATCGCCT CCAACTTGGA GAAGAAGCAG 
AAGAAGTTTG ACCAGCTGCT GGCAGAGGAG AAGAACATCT CCGCCCGCTA TGCAGAAGAG 
CGAGACCGCG CAGAGGCAGA GGCCAGGGAG AAGGAAACCA AAGCCCTGTC CCTGGCCCGA 
GCCCTCGAGG AGGCCCTAGA AACCAAGGAA GAGTTTGAGA GACAGAACAA GCAGCTGCGG 
GCAGACATGG AGGACCTGAT AAGCTCCAAG GATGACGTGG GGAAGAACGT GCACGAGCTT 
GAAAAATCGA AGCGGGCCCT GGAGCAACAG GTGGAGGAGA TGAGGACACA GCTGGAAGAG 
CTGGAGGATG AGCTTCAGGC CACAGAAGAC GCCAAGCTTC GCCTGGAAGT CAACATGCAG 
GCCATGAAGG CCCAGTTTGA GAGGGACTTA CAAACCAGGG ACGAGCAGAA TGAAGAGAAG 
AAGCGGCTCC TGGTTAAGCA GGTGCGGGAG CTGGAGGCAG AGCTGGAAGA TGAGCGGAAG 
CAGCGAGCGC TGGCGGTCGC TTCTAAAAAG AAACTGGAAA TAGACCTGAA GGACCTTGAA 
GCCCAGATTG AGGCTGCGAA CAAAGCGAGG GACGAAGTGA TCAAGCAGCT TCGCAAGCTT 
CAGGCTCAGA TGAAGGATTA CCAACGTGAA TTAGAAGAAG CCCGTGCATC CCGGGATGAG 
ATTTTTGCTC AATCCAAAGA GAGTGAAAAG AAACTGAAGA GTCTGGAAGC AGAAATCCTT 
CAATTGCAGG AGGAACTGGC CTCATCTGAG CGAGCGCGCC GCCACGCCGA GCAGGAGAGA 
GATGAGCTGG CTGACGAGAT CGCCAACAGC GCCTCTGGCA AGTCTGCGCT GCTGGATGAG 
AAGCGGCGTC TGGAGGCACG GATCGCGCAG CTGGAGGAGG AGCTGGAAGA GGAGCAGAGC 
AACATGGAGC TGCTCAACGA CCGCTTCCGC AAGACCACGC TGCAGGTGGA CACGCTGAAC 
GCCGAGCTCG CGGCTGAGCG CAGCGCCGCC CAGAAGAGCG ACAACGCACG CCAGCAGCTA 
GAGCGGCAGA ACAAGGAGCT GAAGGCCAAG CTGCAGGAAC TGGAGGGCGC CGTCAAGTCC 
AAGTTCAAGG CCACCATCTC TGCCCTGGAA GCCAAGATTG GGCAGCTGGA GGAGCAGCTG 
GAGCAGGAAG CCAAGGAACG AGCAGCCGCC AACAAACTAG TCCGTCGCAC GGAGAAAAAG 
CTGAAAGAGA TCTTCATGCA GGTTGAAGAT GAGCGTCGAC ATGCGGATCA GTATAAAGAA 
CAGATGGAGA AGGCCAACGC CAGGATGAAA CAGCTCAAGC GGCAGCTGGA GGAGGCAGAG 
GAGGAAGCCA CACGTGCCAA CGCATCTCGG CGTAAGCTCC AGCGGGAACT GGACGATGCC 
ACGGAGGCCA ACGAGGGCCT GAGCCGTGAG GTCAGCACGC TGAAGAACCG GCTGAGACGA 
GGTGGCCCCA TAAGCTTCTC GTCCAGCCGA TCGGGCCGGC GCCAGCTACA CATCGAGGGA 
GCCTCCCTGG AGCTGTCAGA CGATGACACG GAGAGTAAGA CCAGTGACGT CAACGAGACA 
CAGCCGCCGC AGTCGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_014113598.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969073.1 Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X5, mRNA.

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CloneID	OCe36460
Clone ID Related Accession (Same CDS sequence)	XM_014113598.1 , XM_014113598.2
Accession Version	XM_014113598.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	myosin-10 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGAATCGC CTAAACCAGT GAAACATCAG 
GGGGAACTTG AACGGCAGCT TTTGCAAGCA AATCCAATCC TGGAATCCTT TGGAAATGCG 
AAGACTGTGA AAAATGATAA TTCATCTCGC TTTGGCAAGT TTATTCGAAT CAACTTTGAT 
GTAACTGGCT ATATTGTTGG GGCCAACATT GAAACATACC TTCTGGAAAA ATCTCGTGCT 
GTTCGTCAAG CAAAAGATGA GCGTACTTTT CATATCTTTT ACCAGTTGTT ATCTGGAGCA 
GGAGAACACC TAAAGTCGGA TTTGCTCCTC GAAGGATTTA ATAACTACAG ATTCCTCTCC 
AACGGCTATA TTCCCATCCC GGGACAGCAA GACAAGGACA ACTTTCAGGA AACCATGGAA 
GCAATGCACA TCATGGGCTT CTCTCACGAG GAGATTCTGT CAATGCTTAA AGTAGTGTCA 
TCAGTGCTAC AGTTCGGAAA TATTTCTTTC AAAAAGGAGC GAAATACTGA TCAAGCATCC 
ATGCCAGAGA ACACAGTTGC ACAGAAACTC TGCCATCTTC TTGGGATGAA CGTGATGGAG 
TTCACGCGGG CCATCCTCAC CCCCCGGATC AAGGTCGGCC GAGACTATGT GCAGAAAGCC 
CAGACCAAAG AACAAGCAGA TTTTGCGGTA GAAGCATTGG CAAAAGCTAC CTATGAGCGG 
CTCTTCCGTT GGCTCGTTCA CCGTATCAAC AAAGCTCTGG ACAGGACCAA GCGTCAGGGC 
GCATCTTTTA TTGGAATCCT GGATATCGCT GGATTCGAAA TATTTGAGCT CAACTCGTTC 
GAGCAACTGT GCATCAACTA CACCAATGAG AAGCTGCAGC AGCTCTTCAA CCACACCATG 
TTCATCCTGG AGCAGGAGGA GTACCAGCGC GAGGGCATCG AGTGGAGCTT CATTGACTTT 
GGCCTCGACC TGCAGCCCTG CATCGACCTC ATCGAGAGAC CCGCTAACCC CCCTGGTGTG 
CTGGCCCTTT TGGATGAAGA GTGCTGGTTC CCGAAGGCCA CGGACAAAAC CTTTGTTGAA 
AAACTAGTTC AAGAGCAAGG CTCTCACTCC AAGTTCCAGA AACCCCGGCA ACTGAAAGAC 
AGAGCTGACT TCTGCATCAT CCATTATGCG GGCAAGGTGG ACTACAAGGC TGATGAGTGG 
CTGATGAAGA ACATGGACCC CCTGAACGAC AATGTGGCCA CCCTCCTGCA CCAGTCATCT 
GACAGATTCG TGGCAGAGCT TTGGAAGGAT GTGGACCGTA TCGTGGGTCT GGATCAGGTC 
ACGGGCATGA CGGAGACAGC CTTTGGCTCT GCGTATAAAA CCAAGAAGGG CATGTTTCGG 
ACTGTCGGGC AGCTCTACAA AGAATCCCTC ACCAAGCTGA TGGCAACTCT CCGGAACACC 
AACCCTAACT TCGTCCGCTG CATCATTCCA AATCACGAGA AGCGAGCTGG AAAACTGGAT 
CCGCACCTAG TGCTTGATCA GCTTCGCTGT AACGGCGTTC TGGAAGGAAT CAGGATCTGT 
CGCCAGGGGT TCCCCAACCG GATAGTTTTC CAGGAATTCA GACAGAGATA CGAGATCCTA 
ACTCCAAATG CTATCCCTAA GGGTTTCATG GACGGCAAAC AGGCCTGCGA ACGAATGATC 
CGGGCTTTAG AACTGGACCC AAACTTGTAC AGAATCGGAC AGAGCAAGAT ATTTTTCCGA 
GCTGGCGTGC TGGCACACTT AGAGGAAGAG AGGGATCTAA AGATCACCGA CATCATTATC 
TTCTTCCAGG CTGTCTGCAG AGGCTACCTG GCCAGAAAGG CCTTCGCCAA GAAGCAGCAG 
CAGCTGAGCG CCCTGAAGGT CCTGCAGCGG AACTGCGCCG CATACCTGAA GCTCCGGCAC 
TGGCAGTGGT GGCGTGTCTT CACGAAGGTG AAGCCGCTCC TGCAAGTGAC CCGCCAGGAG 
GAAGAGCTTC AGGCCAAAGA CGAGGAGCTG CTGAAGGTGA AGGAGAGGCA GACGAAGGTG 
GAGGGCGAGC TGGAGGAAAT GGAGAGGAAG CACCAGCAGC TCCTGGAAGA GAAGAACATC 
CTAACAGAGC AGCTCCAAGC AGAGACAGAG CTCTTTGCTG AGGCAGAAGA GATGAGAGCC 
AGGCTCGCCG CCAAGAAGCA GGAGCTGGAG GAGATTCTCC ATGACTTGGA ATCCAGGGTC 
GAAGAGGAAG AAGAACGAAA CCAAATCCTC CAAAATGAAA AGAAGAAAAT GCAAGCTCAC 
ATTCAGGACC TAGAGGAGCA ACTGGATGAG GAAGAAGGCG CTCGCCAGAA ACTACAGCTG 
GAAAAGGTGA CAGCGGAAGC AAAAATCAAG AAGATGGAGG AGGAGATCCT GCTGCTTGAG 
GACCAGAATT CCAAATTTAT CAAAGAAAAG AAACTCATGG AAGATCGCAT TGCAGAGTGT 
TCCTCTCAGC TGGCTGAGGA GGAAGAAAAG GCCAAAAACT TGGCCAAAAT CAGGAATAAG 
CAGGAAGTGA TGATCTCAGA TTTAGAAGAA CGCTTGAAGA AGGAAGAGAA GACGCGTCAG 
GAGCTGGAGA AGGCCAAACG AAAACTGGAC GGTGAGACCA CTGACCTGCA GGACCAGATC 
GCAGAGCTCC AGGCGCAGAT CGACGAGCTC AAGGTCCAGC TGGCCAAGAA GGAGGAGGAG 
CTGCAAGGCG CGCTGGCCAG GGGTGACGAT GAGACGCTCC ATAAGAACAA TGCCCTCAAG 
GTGGTGCGAG AGCTGCAAGC CCAAATCGCC GAGCTTCAGG AGGACTTTGA ATCAGAGAAG 
GCTTCACGGA ACAAGGCGGA GAAGCAGAAG AGGGACCTGA GTGAGGAGCT GGAGGCCCTG 
AAAACAGAGC TGGAGGACAC GCTGGACACC ACCGCGGCTC AGCAGGAACT TCGCACGAAG 
CGTGAACAAG AAGTGGCAGA GCTGAAGAAG GCTCTGGAGG AGGAAACGAG GAGCCACGAG 
GCTCAAATCC AGGACATGAG ACAGCGACAC GCCACAGCCC TGGAGGAGCT GTCGGAGCAG 
CTGGAACAGG CAAAGAGGTT CAAAGCGAAT CTGGAGAAGA ACAAGCAGGG CCTGGAGACT 
GACAACAAGG AGCTGGCGTG TGAGGTGAAG GTGCTACAGC AGGTCAAGGC TGAGTCTGAG 
CACAAGAGGA AGAAGCTCGA CGCCCAGGTG CAGGAGCTGC ACGCCAAGGT CTCAGAAGGA 
GACAGGCTCA GGGTGGAGCT GGCCGAGAAG GCAAACAAAT TACAGAATGA ACTGGACAAT 
GTCTCAAGTC TTCTGGAAGA AGCAGAGAAG AAGGGCATTA AGTTTGCTAA GGACGCAGCT 
AGTCTTGAGT CTCAACTACA GGACACTCAG GAGCTCCTTC AGGAGGAGAC CCGCCAGAAG 
CTGAACCTGA GCAGCCGCAT CCGGCAGCTG GAGGAGGAGA AGAACAGTCT CCAGGAGCAG 
CAGGAGGAGG AGGAGGAGGC CAGGAAAAAC CTGGAAAAGC AGGTGTTGGT GCTGCAGTCC 
CAGCTCGCCG ATACCAAGAA GAAGGTAGAT GATGACTTAG GAACCATCGA GAGCTTAGAA 
GAAGCCAGAA AGAAGCTTCT GAAAGATGTG GAGGCCCTGG GCCAGCGCCT GGAGGAGAAG 
GCTCTGGCCT ATGACAAGCT GGAGAAGACC AAGAACCGCC TGCAGCAGGA GCTCGACGAC 
CTGACAGTGG ACCTAGATCA CCAGCGCCAG ATCGCCTCCA ACTTGGAGAA GAAGCAGAAG 
AAGTTTGACC AGCTGCTGGC AGAGGAGAAG AACATCTCCG CCCGCTATGC AGAAGAGCGA 
GACCGCGCAG AGGCAGAGGC CAGGGAGAAG GAAACCAAAG CCCTGTCCCT GGCCCGAGCC 
CTCGAGGAGG CCCTAGAAAC CAAGGAAGAG TTTGAGAGAC AGAACAAGCA GCTGCGGGCA 
GACATGGAGG ACCTGATAAG CTCCAAGGAT GACGTGGGGA AGAACGTGCA CGAGCTTGAA 
AAATCGAAGC GGGCCCTGGA GCAACAGGTG GAGGAGATGA GGACACAGCT GGAAGAGCTG 
GAGGATGAGC TTCAGGCCAC AGAAGACGCC AAGCTTCGCC TGGAAGTCAA CATGCAGGCC 
ATGAAGGCCC AGTTTGAGAG GGACTTACAA ACCAGGGACG AGCAGAATGA AGAGAAGAAG 
CGGCTCCTGG TTAAGCAGGT GCGGGAGCTG GAGGCAGAGC TGGAAGATGA GCGGAAGCAG 
CGAGCGCTGG CGGTCGCTTC TAAAAAGAAA CTGGAAATAG ACCTGAAGGA CCTTGAAGCC 
CAGATTGAGG CTGCGAACAA AGCGAGGGAC GAAGTGATCA AGCAGCTTCG CAAGCTTCAG 
GCTCAGATGA AGGATTACCA ACGTGAATTA GAAGAAGCCC GTGCATCCCG GGATGAGATT 
TTTGCTCAAT CCAAAGAGAG TGAAAAGAAA CTGAAGAGTC TGGAAGCAGA AATCCTTCAA 
TTGCAGGAGG AACTGGCCTC ATCTGAGCGA GCGCGCCGCC ACGCCGAGCA GGAGAGAGAT 
GAGCTGGCTG ACGAGATCGC CAACAGCGCC TCTGGCAAGT CTGCGCTGCT GGATGAGAAG 
CGGCGTCTGG AGGCACGGAT CGCGCAGCTG GAGGAGGAGC TGGAAGAGGA GCAGAGCAAC 
ATGGAGCTGC TCAACGACCG CTTCCGCAAG ACCACGCTGC AGGTGGACAC GCTGAACGCC 
GAGCTCGCGG CTGAGCGCAG CGCCGCCCAG AAGAGCGACA ACGCACGCCA GCAGCTAGAG 
CGGCAGAACA AGGAGCTGAA GGCCAAGCTG CAGGAACTGG AGGGCGCCGT CAAGTCCAAG 
TTCAAGGCCA CCATCTCTGC CCTGGAAGCC AAGATTGGGC AGCTGGAGGA GCAGCTGGAG 
CAGGAAGCCA AGGAACGAGC AGCCGCCAAC AAACTAGTCC GTCGCACGGA GAAAAAGCTG 
AAAGAGATCT TCATGCAGGT TGAAGATGAG CGTCGACATG CGGATCAGTA TAAAGAACAG 
ATGGAGAAGG CCAACGCCAG GATGAAACAG CTCAAGCGGC AGCTGGAGGA GGCAGAGGAG 
GAAGCCACAC GTGCCAACGC ATCTCGGCGT AAGCTCCAGC GGGAACTGGA CGATGCCACG 
GAGGCCAACG AGGGCCTGAG CCGTGAGGTC AGCACGCTGA AGAACCGGCT GAGACGAGGT 
GGCCCCATAA GCTTCTCGTC CAGCCGATCG GGCCGGCGCC AGCTACACAT CGAGGGAGCC 
TCCCTGGAGC TGTCAGACGA TGACACGGAG AGTAAGACCA GTGACGTCAA CGAGACACAG 
CCGCCGCAGT CGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969073.1
CDS	132..6089
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_014113598.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113598.1

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGAATCGC CTAAACCAGT GAAACATCAG 
GGGGAACTTG AACGGCAGCT TTTGCAAGCA AATCCAATCC TGGAATCCTT TGGAAATGCG 
AAGACTGTGA AAAATGATAA TTCATCTCGC TTTGGCAAGT TTATTCGAAT CAACTTTGAT 
GTAACTGGCT ATATTGTTGG GGCCAACATT GAAACATACC TTCTGGAAAA ATCTCGTGCT 
GTTCGTCAAG CAAAAGATGA GCGTACTTTT CATATCTTTT ACCAGTTGTT ATCTGGAGCA 
GGAGAACACC TAAAGTCGGA TTTGCTCCTC GAAGGATTTA ATAACTACAG ATTCCTCTCC 
AACGGCTATA TTCCCATCCC GGGACAGCAA GACAAGGACA ACTTTCAGGA AACCATGGAA 
GCAATGCACA TCATGGGCTT CTCTCACGAG GAGATTCTGT CAATGCTTAA AGTAGTGTCA 
TCAGTGCTAC AGTTCGGAAA TATTTCTTTC AAAAAGGAGC GAAATACTGA TCAAGCATCC 
ATGCCAGAGA ACACAGTTGC ACAGAAACTC TGCCATCTTC TTGGGATGAA CGTGATGGAG 
TTCACGCGGG CCATCCTCAC CCCCCGGATC AAGGTCGGCC GAGACTATGT GCAGAAAGCC 
CAGACCAAAG AACAAGCAGA TTTTGCGGTA GAAGCATTGG CAAAAGCTAC CTATGAGCGG 
CTCTTCCGTT GGCTCGTTCA CCGTATCAAC AAAGCTCTGG ACAGGACCAA GCGTCAGGGC 
GCATCTTTTA TTGGAATCCT GGATATCGCT GGATTCGAAA TATTTGAGCT CAACTCGTTC 
GAGCAACTGT GCATCAACTA CACCAATGAG AAGCTGCAGC AGCTCTTCAA CCACACCATG 
TTCATCCTGG AGCAGGAGGA GTACCAGCGC GAGGGCATCG AGTGGAGCTT CATTGACTTT 
GGCCTCGACC TGCAGCCCTG CATCGACCTC ATCGAGAGAC CCGCTAACCC CCCTGGTGTG 
CTGGCCCTTT TGGATGAAGA GTGCTGGTTC CCGAAGGCCA CGGACAAAAC CTTTGTTGAA 
AAACTAGTTC AAGAGCAAGG CTCTCACTCC AAGTTCCAGA AACCCCGGCA ACTGAAAGAC 
AGAGCTGACT TCTGCATCAT CCATTATGCG GGCAAGGTGG ACTACAAGGC TGATGAGTGG 
CTGATGAAGA ACATGGACCC CCTGAACGAC AATGTGGCCA CCCTCCTGCA CCAGTCATCT 
GACAGATTCG TGGCAGAGCT TTGGAAGGAT GTGGACCGTA TCGTGGGTCT GGATCAGGTC 
ACGGGCATGA CGGAGACAGC CTTTGGCTCT GCGTATAAAA CCAAGAAGGG CATGTTTCGG 
ACTGTCGGGC AGCTCTACAA AGAATCCCTC ACCAAGCTGA TGGCAACTCT CCGGAACACC 
AACCCTAACT TCGTCCGCTG CATCATTCCA AATCACGAGA AGCGAGCTGG AAAACTGGAT 
CCGCACCTAG TGCTTGATCA GCTTCGCTGT AACGGCGTTC TGGAAGGAAT CAGGATCTGT 
CGCCAGGGGT TCCCCAACCG GATAGTTTTC CAGGAATTCA GACAGAGATA CGAGATCCTA 
ACTCCAAATG CTATCCCTAA GGGTTTCATG GACGGCAAAC AGGCCTGCGA ACGAATGATC 
CGGGCTTTAG AACTGGACCC AAACTTGTAC AGAATCGGAC AGAGCAAGAT ATTTTTCCGA 
GCTGGCGTGC TGGCACACTT AGAGGAAGAG AGGGATCTAA AGATCACCGA CATCATTATC 
TTCTTCCAGG CTGTCTGCAG AGGCTACCTG GCCAGAAAGG CCTTCGCCAA GAAGCAGCAG 
CAGCTGAGCG CCCTGAAGGT CCTGCAGCGG AACTGCGCCG CATACCTGAA GCTCCGGCAC 
TGGCAGTGGT GGCGTGTCTT CACGAAGGTG AAGCCGCTCC TGCAAGTGAC CCGCCAGGAG 
GAAGAGCTTC AGGCCAAAGA CGAGGAGCTG CTGAAGGTGA AGGAGAGGCA GACGAAGGTG 
GAGGGCGAGC TGGAGGAAAT GGAGAGGAAG CACCAGCAGC TCCTGGAAGA GAAGAACATC 
CTAACAGAGC AGCTCCAAGC AGAGACAGAG CTCTTTGCTG AGGCAGAAGA GATGAGAGCC 
AGGCTCGCCG CCAAGAAGCA GGAGCTGGAG GAGATTCTCC ATGACTTGGA ATCCAGGGTC 
GAAGAGGAAG AAGAACGAAA CCAAATCCTC CAAAATGAAA AGAAGAAAAT GCAAGCTCAC 
ATTCAGGACC TAGAGGAGCA ACTGGATGAG GAAGAAGGCG CTCGCCAGAA ACTACAGCTG 
GAAAAGGTGA CAGCGGAAGC AAAAATCAAG AAGATGGAGG AGGAGATCCT GCTGCTTGAG 
GACCAGAATT CCAAATTTAT CAAAGAAAAG AAACTCATGG AAGATCGCAT TGCAGAGTGT 
TCCTCTCAGC TGGCTGAGGA GGAAGAAAAG GCCAAAAACT TGGCCAAAAT CAGGAATAAG 
CAGGAAGTGA TGATCTCAGA TTTAGAAGAA CGCTTGAAGA AGGAAGAGAA GACGCGTCAG 
GAGCTGGAGA AGGCCAAACG AAAACTGGAC GGTGAGACCA CTGACCTGCA GGACCAGATC 
GCAGAGCTCC AGGCGCAGAT CGACGAGCTC AAGGTCCAGC TGGCCAAGAA GGAGGAGGAG 
CTGCAAGGCG CGCTGGCCAG GGGTGACGAT GAGACGCTCC ATAAGAACAA TGCCCTCAAG 
GTGGTGCGAG AGCTGCAAGC CCAAATCGCC GAGCTTCAGG AGGACTTTGA ATCAGAGAAG 
GCTTCACGGA ACAAGGCGGA GAAGCAGAAG AGGGACCTGA GTGAGGAGCT GGAGGCCCTG 
AAAACAGAGC TGGAGGACAC GCTGGACACC ACCGCGGCTC AGCAGGAACT TCGCACGAAG 
CGTGAACAAG AAGTGGCAGA GCTGAAGAAG GCTCTGGAGG AGGAAACGAG GAGCCACGAG 
GCTCAAATCC AGGACATGAG ACAGCGACAC GCCACAGCCC TGGAGGAGCT GTCGGAGCAG 
CTGGAACAGG CAAAGAGGTT CAAAGCGAAT CTGGAGAAGA ACAAGCAGGG CCTGGAGACT 
GACAACAAGG AGCTGGCGTG TGAGGTGAAG GTGCTACAGC AGGTCAAGGC TGAGTCTGAG 
CACAAGAGGA AGAAGCTCGA CGCCCAGGTG CAGGAGCTGC ACGCCAAGGT CTCAGAAGGA 
GACAGGCTCA GGGTGGAGCT GGCCGAGAAG GCAAACAAAT TACAGAATGA ACTGGACAAT 
GTCTCAAGTC TTCTGGAAGA AGCAGAGAAG AAGGGCATTA AGTTTGCTAA GGACGCAGCT 
AGTCTTGAGT CTCAACTACA GGACACTCAG GAGCTCCTTC AGGAGGAGAC CCGCCAGAAG 
CTGAACCTGA GCAGCCGCAT CCGGCAGCTG GAGGAGGAGA AGAACAGTCT CCAGGAGCAG 
CAGGAGGAGG AGGAGGAGGC CAGGAAAAAC CTGGAAAAGC AGGTGTTGGT GCTGCAGTCC 
CAGCTCGCCG ATACCAAGAA GAAGGTAGAT GATGACTTAG GAACCATCGA GAGCTTAGAA 
GAAGCCAGAA AGAAGCTTCT GAAAGATGTG GAGGCCCTGG GCCAGCGCCT GGAGGAGAAG 
GCTCTGGCCT ATGACAAGCT GGAGAAGACC AAGAACCGCC TGCAGCAGGA GCTCGACGAC 
CTGACAGTGG ACCTAGATCA CCAGCGCCAG ATCGCCTCCA ACTTGGAGAA GAAGCAGAAG 
AAGTTTGACC AGCTGCTGGC AGAGGAGAAG AACATCTCCG CCCGCTATGC AGAAGAGCGA 
GACCGCGCAG AGGCAGAGGC CAGGGAGAAG GAAACCAAAG CCCTGTCCCT GGCCCGAGCC 
CTCGAGGAGG CCCTAGAAAC CAAGGAAGAG TTTGAGAGAC AGAACAAGCA GCTGCGGGCA 
GACATGGAGG ACCTGATAAG CTCCAAGGAT GACGTGGGGA AGAACGTGCA CGAGCTTGAA 
AAATCGAAGC GGGCCCTGGA GCAACAGGTG GAGGAGATGA GGACACAGCT GGAAGAGCTG 
GAGGATGAGC TTCAGGCCAC AGAAGACGCC AAGCTTCGCC TGGAAGTCAA CATGCAGGCC 
ATGAAGGCCC AGTTTGAGAG GGACTTACAA ACCAGGGACG AGCAGAATGA AGAGAAGAAG 
CGGCTCCTGG TTAAGCAGGT GCGGGAGCTG GAGGCAGAGC TGGAAGATGA GCGGAAGCAG 
CGAGCGCTGG CGGTCGCTTC TAAAAAGAAA CTGGAAATAG ACCTGAAGGA CCTTGAAGCC 
CAGATTGAGG CTGCGAACAA AGCGAGGGAC GAAGTGATCA AGCAGCTTCG CAAGCTTCAG 
GCTCAGATGA AGGATTACCA ACGTGAATTA GAAGAAGCCC GTGCATCCCG GGATGAGATT 
TTTGCTCAAT CCAAAGAGAG TGAAAAGAAA CTGAAGAGTC TGGAAGCAGA AATCCTTCAA 
TTGCAGGAGG AACTGGCCTC ATCTGAGCGA GCGCGCCGCC ACGCCGAGCA GGAGAGAGAT 
GAGCTGGCTG ACGAGATCGC CAACAGCGCC TCTGGCAAGT CTGCGCTGCT GGATGAGAAG 
CGGCGTCTGG AGGCACGGAT CGCGCAGCTG GAGGAGGAGC TGGAAGAGGA GCAGAGCAAC 
ATGGAGCTGC TCAACGACCG CTTCCGCAAG ACCACGCTGC AGGTGGACAC GCTGAACGCC 
GAGCTCGCGG CTGAGCGCAG CGCCGCCCAG AAGAGCGACA ACGCACGCCA GCAGCTAGAG 
CGGCAGAACA AGGAGCTGAA GGCCAAGCTG CAGGAACTGG AGGGCGCCGT CAAGTCCAAG 
TTCAAGGCCA CCATCTCTGC CCTGGAAGCC AAGATTGGGC AGCTGGAGGA GCAGCTGGAG 
CAGGAAGCCA AGGAACGAGC AGCCGCCAAC AAACTAGTCC GTCGCACGGA GAAAAAGCTG 
AAAGAGATCT TCATGCAGGT TGAAGATGAG CGTCGACATG CGGATCAGTA TAAAGAACAG 
ATGGAGAAGG CCAACGCCAG GATGAAACAG CTCAAGCGGC AGCTGGAGGA GGCAGAGGAG 
GAAGCCACAC GTGCCAACGC ATCTCGGCGT AAGCTCCAGC GGGAACTGGA CGATGCCACG 
GAGGCCAACG AGGGCCTGAG CCGTGAGGTC AGCACGCTGA AGAACCGGCT GAGACGAGGT 
GGCCCCATAA GCTTCTCGTC CAGCCGATCG GGCCGGCGCC AGCTACACAT CGAGGGAGCC 
TCCCTGGAGC TGTCAGACGA TGACACGGAG AGTAAGACCA GTGACGTCAA CGAGACACAG 
CCGCCGCAGT CGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_014113598.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969073.1 Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X5, mRNA.

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CloneID	OCe36460
Clone ID Related Accession (Same CDS sequence)	XM_014113598.1 , XM_014113598.2
Accession Version	XM_014113598.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	myosin-10 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113598.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGAATCGC CTAAACCAGT GAAACATCAG 
GGGGAACTTG AACGGCAGCT TTTGCAAGCA AATCCAATCC TGGAATCCTT TGGAAATGCG 
AAGACTGTGA AAAATGATAA TTCATCTCGC TTTGGCAAGT TTATTCGAAT CAACTTTGAT 
GTAACTGGCT ATATTGTTGG GGCCAACATT GAAACATACC TTCTGGAAAA ATCTCGTGCT 
GTTCGTCAAG CAAAAGATGA GCGTACTTTT CATATCTTTT ACCAGTTGTT ATCTGGAGCA 
GGAGAACACC TAAAGTCGGA TTTGCTCCTC GAAGGATTTA ATAACTACAG ATTCCTCTCC 
AACGGCTATA TTCCCATCCC GGGACAGCAA GACAAGGACA ACTTTCAGGA AACCATGGAA 
GCAATGCACA TCATGGGCTT CTCTCACGAG GAGATTCTGT CAATGCTTAA AGTAGTGTCA 
TCAGTGCTAC AGTTCGGAAA TATTTCTTTC AAAAAGGAGC GAAATACTGA TCAAGCATCC 
ATGCCAGAGA ACACAGTTGC ACAGAAACTC TGCCATCTTC TTGGGATGAA CGTGATGGAG 
TTCACGCGGG CCATCCTCAC CCCCCGGATC AAGGTCGGCC GAGACTATGT GCAGAAAGCC 
CAGACCAAAG AACAAGCAGA TTTTGCGGTA GAAGCATTGG CAAAAGCTAC CTATGAGCGG 
CTCTTCCGTT GGCTCGTTCA CCGTATCAAC AAAGCTCTGG ACAGGACCAA GCGTCAGGGC 
GCATCTTTTA TTGGAATCCT GGATATCGCT GGATTCGAAA TATTTGAGCT CAACTCGTTC 
GAGCAACTGT GCATCAACTA CACCAATGAG AAGCTGCAGC AGCTCTTCAA CCACACCATG 
TTCATCCTGG AGCAGGAGGA GTACCAGCGC GAGGGCATCG AGTGGAGCTT CATTGACTTT 
GGCCTCGACC TGCAGCCCTG CATCGACCTC ATCGAGAGAC CCGCTAACCC CCCTGGTGTG 
CTGGCCCTTT TGGATGAAGA GTGCTGGTTC CCGAAGGCCA CGGACAAAAC CTTTGTTGAA 
AAACTAGTTC AAGAGCAAGG CTCTCACTCC AAGTTCCAGA AACCCCGGCA ACTGAAAGAC 
AGAGCTGACT TCTGCATCAT CCATTATGCG GGCAAGGTGG ACTACAAGGC TGATGAGTGG 
CTGATGAAGA ACATGGACCC CCTGAACGAC AATGTGGCCA CCCTCCTGCA CCAGTCATCT 
GACAGATTCG TGGCAGAGCT TTGGAAGGAT GTGGACCGTA TCGTGGGTCT GGATCAGGTC 
ACGGGCATGA CGGAGACAGC CTTTGGCTCT GCGTATAAAA CCAAGAAGGG CATGTTTCGG 
ACTGTCGGGC AGCTCTACAA AGAATCCCTC ACCAAGCTGA TGGCAACTCT CCGGAACACC 
AACCCTAACT TCGTCCGCTG CATCATTCCA AATCACGAGA AGCGAGCTGG AAAACTGGAT 
CCGCACCTAG TGCTTGATCA GCTTCGCTGT AACGGCGTTC TGGAAGGAAT CAGGATCTGT 
CGCCAGGGGT TCCCCAACCG GATAGTTTTC CAGGAATTCA GACAGAGATA CGAGATCCTA 
ACTCCAAATG CTATCCCTAA GGGTTTCATG GACGGCAAAC AGGCCTGCGA ACGAATGATC 
CGGGCTTTAG AACTGGACCC AAACTTGTAC AGAATCGGAC AGAGCAAGAT ATTTTTCCGA 
GCTGGCGTGC TGGCACACTT AGAGGAAGAG AGGGATCTAA AGATCACCGA CATCATTATC 
TTCTTCCAGG CTGTCTGCAG AGGCTACCTG GCCAGAAAGG CCTTCGCCAA GAAGCAGCAG 
CAGCTGAGCG CCCTGAAGGT CCTGCAGCGG AACTGCGCCG CATACCTGAA GCTCCGGCAC 
TGGCAGTGGT GGCGTGTCTT CACGAAGGTG AAGCCGCTCC TGCAAGTGAC CCGCCAGGAG 
GAAGAGCTTC AGGCCAAAGA CGAGGAGCTG CTGAAGGTGA AGGAGAGGCA GACGAAGGTG 
GAGGGCGAGC TGGAGGAAAT GGAGAGGAAG CACCAGCAGC TCCTGGAAGA GAAGAACATC 
CTAACAGAGC AGCTCCAAGC AGAGACAGAG CTCTTTGCTG AGGCAGAAGA GATGAGAGCC 
AGGCTCGCCG CCAAGAAGCA GGAGCTGGAG GAGATTCTCC ATGACTTGGA ATCCAGGGTC 
GAAGAGGAAG AAGAACGAAA CCAAATCCTC CAAAATGAAA AGAAGAAAAT GCAAGCTCAC 
ATTCAGGACC TAGAGGAGCA ACTGGATGAG GAAGAAGGCG CTCGCCAGAA ACTACAGCTG 
GAAAAGGTGA CAGCGGAAGC AAAAATCAAG AAGATGGAGG AGGAGATCCT GCTGCTTGAG 
GACCAGAATT CCAAATTTAT CAAAGAAAAG AAACTCATGG AAGATCGCAT TGCAGAGTGT 
TCCTCTCAGC TGGCTGAGGA GGAAGAAAAG GCCAAAAACT TGGCCAAAAT CAGGAATAAG 
CAGGAAGTGA TGATCTCAGA TTTAGAAGAA CGCTTGAAGA AGGAAGAGAA GACGCGTCAG 
GAGCTGGAGA AGGCCAAACG AAAACTGGAC GGTGAGACCA CTGACCTGCA GGACCAGATC 
GCAGAGCTCC AGGCGCAGAT CGACGAGCTC AAGGTCCAGC TGGCCAAGAA GGAGGAGGAG 
CTGCAAGGCG CGCTGGCCAG GGGTGACGAT GAGACGCTCC ATAAGAACAA TGCCCTCAAG 
GTGGTGCGAG AGCTGCAAGC CCAAATCGCC GAGCTTCAGG AGGACTTTGA ATCAGAGAAG 
GCTTCACGGA ACAAGGCGGA GAAGCAGAAG AGGGACCTGA GTGAGGAGCT GGAGGCCCTG 
AAAACAGAGC TGGAGGACAC GCTGGACACC ACCGCGGCTC AGCAGGAACT TCGCACGAAG 
CGTGAACAAG AAGTGGCAGA GCTGAAGAAG GCTCTGGAGG AGGAAACGAG GAGCCACGAG 
GCTCAAATCC AGGACATGAG ACAGCGACAC GCCACAGCCC TGGAGGAGCT GTCGGAGCAG 
CTGGAACAGG CAAAGAGGTT CAAAGCGAAT CTGGAGAAGA ACAAGCAGGG CCTGGAGACT 
GACAACAAGG AGCTGGCGTG TGAGGTGAAG GTGCTACAGC AGGTCAAGGC TGAGTCTGAG 
CACAAGAGGA AGAAGCTCGA CGCCCAGGTG CAGGAGCTGC ACGCCAAGGT CTCAGAAGGA 
GACAGGCTCA GGGTGGAGCT GGCCGAGAAG GCAAACAAAT TACAGAATGA ACTGGACAAT 
GTCTCAAGTC TTCTGGAAGA AGCAGAGAAG AAGGGCATTA AGTTTGCTAA GGACGCAGCT 
AGTCTTGAGT CTCAACTACA GGACACTCAG GAGCTCCTTC AGGAGGAGAC CCGCCAGAAG 
CTGAACCTGA GCAGCCGCAT CCGGCAGCTG GAGGAGGAGA AGAACAGTCT CCAGGAGCAG 
CAGGAGGAGG AGGAGGAGGC CAGGAAAAAC CTGGAAAAGC AGGTGTTGGT GCTGCAGTCC 
CAGCTCGCCG ATACCAAGAA GAAGGTAGAT GATGACTTAG GAACCATCGA GAGCTTAGAA 
GAAGCCAGAA AGAAGCTTCT GAAAGATGTG GAGGCCCTGG GCCAGCGCCT GGAGGAGAAG 
GCTCTGGCCT ATGACAAGCT GGAGAAGACC AAGAACCGCC TGCAGCAGGA GCTCGACGAC 
CTGACAGTGG ACCTAGATCA CCAGCGCCAG ATCGCCTCCA ACTTGGAGAA GAAGCAGAAG 
AAGTTTGACC AGCTGCTGGC AGAGGAGAAG AACATCTCCG CCCGCTATGC AGAAGAGCGA 
GACCGCGCAG AGGCAGAGGC CAGGGAGAAG GAAACCAAAG CCCTGTCCCT GGCCCGAGCC 
CTCGAGGAGG CCCTAGAAAC CAAGGAAGAG TTTGAGAGAC AGAACAAGCA GCTGCGGGCA 
GACATGGAGG ACCTGATAAG CTCCAAGGAT GACGTGGGGA AGAACGTGCA CGAGCTTGAA 
AAATCGAAGC GGGCCCTGGA GCAACAGGTG GAGGAGATGA GGACACAGCT GGAAGAGCTG 
GAGGATGAGC TTCAGGCCAC AGAAGACGCC AAGCTTCGCC TGGAAGTCAA CATGCAGGCC 
ATGAAGGCCC AGTTTGAGAG GGACTTACAA ACCAGGGACG AGCAGAATGA AGAGAAGAAG 
CGGCTCCTGG TTAAGCAGGT GCGGGAGCTG GAGGCAGAGC TGGAAGATGA GCGGAAGCAG 
CGAGCGCTGG CGGTCGCTTC TAAAAAGAAA CTGGAAATAG ACCTGAAGGA CCTTGAAGCC 
CAGATTGAGG CTGCGAACAA AGCGAGGGAC GAAGTGATCA AGCAGCTTCG CAAGCTTCAG 
GCTCAGATGA AGGATTACCA ACGTGAATTA GAAGAAGCCC GTGCATCCCG GGATGAGATT 
TTTGCTCAAT CCAAAGAGAG TGAAAAGAAA CTGAAGAGTC TGGAAGCAGA AATCCTTCAA 
TTGCAGGAGG AACTGGCCTC ATCTGAGCGA GCGCGCCGCC ACGCCGAGCA GGAGAGAGAT 
GAGCTGGCTG ACGAGATCGC CAACAGCGCC TCTGGCAAGT CTGCGCTGCT GGATGAGAAG 
CGGCGTCTGG AGGCACGGAT CGCGCAGCTG GAGGAGGAGC TGGAAGAGGA GCAGAGCAAC 
ATGGAGCTGC TCAACGACCG CTTCCGCAAG ACCACGCTGC AGGTGGACAC GCTGAACGCC 
GAGCTCGCGG CTGAGCGCAG CGCCGCCCAG AAGAGCGACA ACGCACGCCA GCAGCTAGAG 
CGGCAGAACA AGGAGCTGAA GGCCAAGCTG CAGGAACTGG AGGGCGCCGT CAAGTCCAAG 
TTCAAGGCCA CCATCTCTGC CCTGGAAGCC AAGATTGGGC AGCTGGAGGA GCAGCTGGAG 
CAGGAAGCCA AGGAACGAGC AGCCGCCAAC AAACTAGTCC GTCGCACGGA GAAAAAGCTG 
AAAGAGATCT TCATGCAGGT TGAAGATGAG CGTCGACATG CGGATCAGTA TAAAGAACAG 
ATGGAGAAGG CCAACGCCAG GATGAAACAG CTCAAGCGGC AGCTGGAGGA GGCAGAGGAG 
GAAGCCACAC GTGCCAACGC ATCTCGGCGT AAGCTCCAGC GGGAACTGGA CGATGCCACG 
GAGGCCAACG AGGGCCTGAG CCGTGAGGTC AGCACGCTGA AGAACCGGCT GAGACGAGGT 
GGCCCCATAA GCTTCTCGTC CAGCCGATCG GGCCGGCGCC AGCTACACAT CGAGGGAGCC 
TCCCTGGAGC TGTCAGACGA TGACACGGAG AGTAAGACCA GTGACGTCAA CGAGACACAG 
CCGCCGCAGT CGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969073.1
CDS	192..6149
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_014113598.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113598.2

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGAATCGC CTAAACCAGT GAAACATCAG 
GGGGAACTTG AACGGCAGCT TTTGCAAGCA AATCCAATCC TGGAATCCTT TGGAAATGCG 
AAGACTGTGA AAAATGATAA TTCATCTCGC TTTGGCAAGT TTATTCGAAT CAACTTTGAT 
GTAACTGGCT ATATTGTTGG GGCCAACATT GAAACATACC TTCTGGAAAA ATCTCGTGCT 
GTTCGTCAAG CAAAAGATGA GCGTACTTTT CATATCTTTT ACCAGTTGTT ATCTGGAGCA 
GGAGAACACC TAAAGTCGGA TTTGCTCCTC GAAGGATTTA ATAACTACAG ATTCCTCTCC 
AACGGCTATA TTCCCATCCC GGGACAGCAA GACAAGGACA ACTTTCAGGA AACCATGGAA 
GCAATGCACA TCATGGGCTT CTCTCACGAG GAGATTCTGT CAATGCTTAA AGTAGTGTCA 
TCAGTGCTAC AGTTCGGAAA TATTTCTTTC AAAAAGGAGC GAAATACTGA TCAAGCATCC 
ATGCCAGAGA ACACAGTTGC ACAGAAACTC TGCCATCTTC TTGGGATGAA CGTGATGGAG 
TTCACGCGGG CCATCCTCAC CCCCCGGATC AAGGTCGGCC GAGACTATGT GCAGAAAGCC 
CAGACCAAAG AACAAGCAGA TTTTGCGGTA GAAGCATTGG CAAAAGCTAC CTATGAGCGG 
CTCTTCCGTT GGCTCGTTCA CCGTATCAAC AAAGCTCTGG ACAGGACCAA GCGTCAGGGC 
GCATCTTTTA TTGGAATCCT GGATATCGCT GGATTCGAAA TATTTGAGCT CAACTCGTTC 
GAGCAACTGT GCATCAACTA CACCAATGAG AAGCTGCAGC AGCTCTTCAA CCACACCATG 
TTCATCCTGG AGCAGGAGGA GTACCAGCGC GAGGGCATCG AGTGGAGCTT CATTGACTTT 
GGCCTCGACC TGCAGCCCTG CATCGACCTC ATCGAGAGAC CCGCTAACCC CCCTGGTGTG 
CTGGCCCTTT TGGATGAAGA GTGCTGGTTC CCGAAGGCCA CGGACAAAAC CTTTGTTGAA 
AAACTAGTTC AAGAGCAAGG CTCTCACTCC AAGTTCCAGA AACCCCGGCA ACTGAAAGAC 
AGAGCTGACT TCTGCATCAT CCATTATGCG GGCAAGGTGG ACTACAAGGC TGATGAGTGG 
CTGATGAAGA ACATGGACCC CCTGAACGAC AATGTGGCCA CCCTCCTGCA CCAGTCATCT 
GACAGATTCG TGGCAGAGCT TTGGAAGGAT GTGGACCGTA TCGTGGGTCT GGATCAGGTC 
ACGGGCATGA CGGAGACAGC CTTTGGCTCT GCGTATAAAA CCAAGAAGGG CATGTTTCGG 
ACTGTCGGGC AGCTCTACAA AGAATCCCTC ACCAAGCTGA TGGCAACTCT CCGGAACACC 
AACCCTAACT TCGTCCGCTG CATCATTCCA AATCACGAGA AGCGAGCTGG AAAACTGGAT 
CCGCACCTAG TGCTTGATCA GCTTCGCTGT AACGGCGTTC TGGAAGGAAT CAGGATCTGT 
CGCCAGGGGT TCCCCAACCG GATAGTTTTC CAGGAATTCA GACAGAGATA CGAGATCCTA 
ACTCCAAATG CTATCCCTAA GGGTTTCATG GACGGCAAAC AGGCCTGCGA ACGAATGATC 
CGGGCTTTAG AACTGGACCC AAACTTGTAC AGAATCGGAC AGAGCAAGAT ATTTTTCCGA 
GCTGGCGTGC TGGCACACTT AGAGGAAGAG AGGGATCTAA AGATCACCGA CATCATTATC 
TTCTTCCAGG CTGTCTGCAG AGGCTACCTG GCCAGAAAGG CCTTCGCCAA GAAGCAGCAG 
CAGCTGAGCG CCCTGAAGGT CCTGCAGCGG AACTGCGCCG CATACCTGAA GCTCCGGCAC 
TGGCAGTGGT GGCGTGTCTT CACGAAGGTG AAGCCGCTCC TGCAAGTGAC CCGCCAGGAG 
GAAGAGCTTC AGGCCAAAGA CGAGGAGCTG CTGAAGGTGA AGGAGAGGCA GACGAAGGTG 
GAGGGCGAGC TGGAGGAAAT GGAGAGGAAG CACCAGCAGC TCCTGGAAGA GAAGAACATC 
CTAACAGAGC AGCTCCAAGC AGAGACAGAG CTCTTTGCTG AGGCAGAAGA GATGAGAGCC 
AGGCTCGCCG CCAAGAAGCA GGAGCTGGAG GAGATTCTCC ATGACTTGGA ATCCAGGGTC 
GAAGAGGAAG AAGAACGAAA CCAAATCCTC CAAAATGAAA AGAAGAAAAT GCAAGCTCAC 
ATTCAGGACC TAGAGGAGCA ACTGGATGAG GAAGAAGGCG CTCGCCAGAA ACTACAGCTG 
GAAAAGGTGA CAGCGGAAGC AAAAATCAAG AAGATGGAGG AGGAGATCCT GCTGCTTGAG 
GACCAGAATT CCAAATTTAT CAAAGAAAAG AAACTCATGG AAGATCGCAT TGCAGAGTGT 
TCCTCTCAGC TGGCTGAGGA GGAAGAAAAG GCCAAAAACT TGGCCAAAAT CAGGAATAAG 
CAGGAAGTGA TGATCTCAGA TTTAGAAGAA CGCTTGAAGA AGGAAGAGAA GACGCGTCAG 
GAGCTGGAGA AGGCCAAACG AAAACTGGAC GGTGAGACCA CTGACCTGCA GGACCAGATC 
GCAGAGCTCC AGGCGCAGAT CGACGAGCTC AAGGTCCAGC TGGCCAAGAA GGAGGAGGAG 
CTGCAAGGCG CGCTGGCCAG GGGTGACGAT GAGACGCTCC ATAAGAACAA TGCCCTCAAG 
GTGGTGCGAG AGCTGCAAGC CCAAATCGCC GAGCTTCAGG AGGACTTTGA ATCAGAGAAG 
GCTTCACGGA ACAAGGCGGA GAAGCAGAAG AGGGACCTGA GTGAGGAGCT GGAGGCCCTG 
AAAACAGAGC TGGAGGACAC GCTGGACACC ACCGCGGCTC AGCAGGAACT TCGCACGAAG 
CGTGAACAAG AAGTGGCAGA GCTGAAGAAG GCTCTGGAGG AGGAAACGAG GAGCCACGAG 
GCTCAAATCC AGGACATGAG ACAGCGACAC GCCACAGCCC TGGAGGAGCT GTCGGAGCAG 
CTGGAACAGG CAAAGAGGTT CAAAGCGAAT CTGGAGAAGA ACAAGCAGGG CCTGGAGACT 
GACAACAAGG AGCTGGCGTG TGAGGTGAAG GTGCTACAGC AGGTCAAGGC TGAGTCTGAG 
CACAAGAGGA AGAAGCTCGA CGCCCAGGTG CAGGAGCTGC ACGCCAAGGT CTCAGAAGGA 
GACAGGCTCA GGGTGGAGCT GGCCGAGAAG GCAAACAAAT TACAGAATGA ACTGGACAAT 
GTCTCAAGTC TTCTGGAAGA AGCAGAGAAG AAGGGCATTA AGTTTGCTAA GGACGCAGCT 
AGTCTTGAGT CTCAACTACA GGACACTCAG GAGCTCCTTC AGGAGGAGAC CCGCCAGAAG 
CTGAACCTGA GCAGCCGCAT CCGGCAGCTG GAGGAGGAGA AGAACAGTCT CCAGGAGCAG 
CAGGAGGAGG AGGAGGAGGC CAGGAAAAAC CTGGAAAAGC AGGTGTTGGT GCTGCAGTCC 
CAGCTCGCCG ATACCAAGAA GAAGGTAGAT GATGACTTAG GAACCATCGA GAGCTTAGAA 
GAAGCCAGAA AGAAGCTTCT GAAAGATGTG GAGGCCCTGG GCCAGCGCCT GGAGGAGAAG 
GCTCTGGCCT ATGACAAGCT GGAGAAGACC AAGAACCGCC TGCAGCAGGA GCTCGACGAC 
CTGACAGTGG ACCTAGATCA CCAGCGCCAG ATCGCCTCCA ACTTGGAGAA GAAGCAGAAG 
AAGTTTGACC AGCTGCTGGC AGAGGAGAAG AACATCTCCG CCCGCTATGC AGAAGAGCGA 
GACCGCGCAG AGGCAGAGGC CAGGGAGAAG GAAACCAAAG CCCTGTCCCT GGCCCGAGCC 
CTCGAGGAGG CCCTAGAAAC CAAGGAAGAG TTTGAGAGAC AGAACAAGCA GCTGCGGGCA 
GACATGGAGG ACCTGATAAG CTCCAAGGAT GACGTGGGGA AGAACGTGCA CGAGCTTGAA 
AAATCGAAGC GGGCCCTGGA GCAACAGGTG GAGGAGATGA GGACACAGCT GGAAGAGCTG 
GAGGATGAGC TTCAGGCCAC AGAAGACGCC AAGCTTCGCC TGGAAGTCAA CATGCAGGCC 
ATGAAGGCCC AGTTTGAGAG GGACTTACAA ACCAGGGACG AGCAGAATGA AGAGAAGAAG 
CGGCTCCTGG TTAAGCAGGT GCGGGAGCTG GAGGCAGAGC TGGAAGATGA GCGGAAGCAG 
CGAGCGCTGG CGGTCGCTTC TAAAAAGAAA CTGGAAATAG ACCTGAAGGA CCTTGAAGCC 
CAGATTGAGG CTGCGAACAA AGCGAGGGAC GAAGTGATCA AGCAGCTTCG CAAGCTTCAG 
GCTCAGATGA AGGATTACCA ACGTGAATTA GAAGAAGCCC GTGCATCCCG GGATGAGATT 
TTTGCTCAAT CCAAAGAGAG TGAAAAGAAA CTGAAGAGTC TGGAAGCAGA AATCCTTCAA 
TTGCAGGAGG AACTGGCCTC ATCTGAGCGA GCGCGCCGCC ACGCCGAGCA GGAGAGAGAT 
GAGCTGGCTG ACGAGATCGC CAACAGCGCC TCTGGCAAGT CTGCGCTGCT GGATGAGAAG 
CGGCGTCTGG AGGCACGGAT CGCGCAGCTG GAGGAGGAGC TGGAAGAGGA GCAGAGCAAC 
ATGGAGCTGC TCAACGACCG CTTCCGCAAG ACCACGCTGC AGGTGGACAC GCTGAACGCC 
GAGCTCGCGG CTGAGCGCAG CGCCGCCCAG AAGAGCGACA ACGCACGCCA GCAGCTAGAG 
CGGCAGAACA AGGAGCTGAA GGCCAAGCTG CAGGAACTGG AGGGCGCCGT CAAGTCCAAG 
TTCAAGGCCA CCATCTCTGC CCTGGAAGCC AAGATTGGGC AGCTGGAGGA GCAGCTGGAG 
CAGGAAGCCA AGGAACGAGC AGCCGCCAAC AAACTAGTCC GTCGCACGGA GAAAAAGCTG 
AAAGAGATCT TCATGCAGGT TGAAGATGAG CGTCGACATG CGGATCAGTA TAAAGAACAG 
ATGGAGAAGG CCAACGCCAG GATGAAACAG CTCAAGCGGC AGCTGGAGGA GGCAGAGGAG 
GAAGCCACAC GTGCCAACGC ATCTCGGCGT AAGCTCCAGC GGGAACTGGA CGATGCCACG 
GAGGCCAACG AGGGCCTGAG CCGTGAGGTC AGCACGCTGA AGAACCGGCT GAGACGAGGT 
GGCCCCATAA GCTTCTCGTC CAGCCGATCG GGCCGGCGCC AGCTACACAT CGAGGGAGCC 
TCCCTGGAGC TGTCAGACGA TGACACGGAG AGTAAGACCA GTGACGTCAA CGAGACACAG 
CCGCCGCAGT CGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_014113599.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969074.1 Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X6, mRNA.

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CloneID	OCe36461
Clone ID Related Accession (Same CDS sequence)	XM_014113599.1 , XM_014113599.2
Accession Version	XM_014113599.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	myosin-10 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC CTTTGGAAAT GCGAAGACTG TGAAAAATGA TAATTCATCT 
CGCTTTGGCA AGTTTATTCG AATCAACTTT GATGTAACTG GCTATATTGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAGTT GTTATCTGGA GCAGGAGAAC ACCTAAAGTC GGATTTGCTC 
CTCGAAGGAT TTAATAACTA CAGATTCCTC TCCAACGGCT ATATTCCCAT CCCGGGACAG 
CAAGACAAGG ACAACTTTCA GGAAACCATG GAAGCAATGC ACATCATGGG CTTCTCTCAC 
GAGGAGATTC TGTCAATGCT TAAAGTAGTG TCATCAGTGC TACAGTTCGG AAATATTTCT 
TTCAAAAAGG AGCGAAATAC TGATCAAGCA TCCATGCCAG AGAACACAGT TGCACAGAAA 
CTCTGCCATC TTCTTGGGAT GAACGTGATG GAGTTCACGC GGGCCATCCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA TGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCG 
GTAGAAGCAT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GTTGGCTCGT TCACCGTATC 
AACAAAGCTC TGGACAGGAC CAAGCGTCAG GGCGCATCTT TTATTGGAAT CCTGGATATC 
GCTGGATTCG AAATATTTGA GCTCAACTCG TTCGAGCAAC TGTGCATCAA CTACACCAAT 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAG CTTCATTGAC TTTGGCCTCG ACCTGCAGCC CTGCATCGAC 
CTCATCGAGA GACCCGCTAA CCCCCCTGGT GTGCTGGCCC TTTTGGATGA AGAGTGCTGG 
TTCCCGAAGG CCACGGACAA AACCTTTGTT GAAAAACTAG TTCAAGAGCA AGGCTCTCAC 
TCCAAGTTCC AGAAACCCCG GCAACTGAAA GACAGAGCTG ACTTCTGCAT CATCCATTAT 
GCGGGCAAGG TGGACTACAA GGCTGATGAG TGGCTGATGA AGAACATGGA CCCCCTGAAC 
GACAATGTGG CCACCCTCCT GCACCAGTCA TCTGACAGAT TCGTGGCAGA GCTTTGGAAG 
GATGTGGACC GTATCGTGGG TCTGGATCAG GTCACGGGCA TGACGGAGAC AGCCTTTGGC 
TCTGCGTATA AAACCAAGAA GGGCATGTTT CGGACTGTCG GGCAGCTCTA CAAAGAATCC 
CTCACCAAGC TGATGGCAAC TCTCCGGAAC ACCAACCCTA ACTTCGTCCG CTGCATCATT 
CCAAATCACG AGAAGCGAGC TGGAAAACTG GATCCGCACC TAGTGCTTGA TCAGCTTCGC 
TGTAACGGCG TTCTGGAAGG AATCAGGATC TGTCGCCAGG GGTTCCCCAA CCGGATAGTT 
TTCCAGGAAT TCAGACAGAG ATACGAGATC CTAACTCCAA ATGCTATCCC TAAGGGTTTC 
ATGGACGGCA AACAGGCCTG CGAACGAATG ATCCGGGCTT TAGAACTGGA CCCAAACTTG 
TACAGAATCG GACAGAGCAA GATATTTTTC CGAGCTGGCG TGCTGGCACA CTTAGAGGAA 
GAGAGGGATC TAAAGATCAC CGACATCATT ATCTTCTTCC AGGCTGTCTG CAGAGGCTAC 
CTGGCCAGAA AGGCCTTCGC CAAGAAGCAG CAGCAGCTGA GCGCCCTGAA GGTCCTGCAG 
CGGAACTGCG CCGCATACCT GAAGCTCCGG CACTGGCAGT GGTGGCGTGT CTTCACGAAG 
GTGAAGCCGC TCCTGCAAGT GACCCGCCAG GAGGAAGAGC TTCAGGCCAA AGACGAGGAG 
CTGCTGAAGG TGAAGGAGAG GCAGACGAAG GTGGAGGGCG AGCTGGAGGA AATGGAGAGG 
AAGCACCAGC AGCTCCTGGA AGAGAAGAAC ATCCTAACAG AGCAGCTCCA AGCAGAGACA 
GAGCTCTTTG CTGAGGCAGA AGAGATGAGA GCCAGGCTCG CCGCCAAGAA GCAGGAGCTG 
GAGGAGATTC TCCATGACTT GGAATCCAGG GTCGAAGAGG AAGAAGAACG AAACCAAATC 
CTCCAAAATG AAAAGAAGAA AATGCAAGCT CACATTCAGG ACCTAGAGGA GCAACTGGAT 
GAGGAAGAAG GCGCTCGCCA GAAACTACAG CTGGAAAAGG TGACAGCGGA AGCAAAAATC 
AAGAAGATGG AGGAGGAGAT CCTGCTGCTT GAGGACCAGA ATTCCAAATT TATCAAAGAA 
AAGAAACTCA TGGAAGATCG CATTGCAGAG TGTTCCTCTC AGCTGGCTGA GGAGGAAGAA 
AAGGCCAAAA ACTTGGCCAA AATCAGGAAT AAGCAGGAAG TGATGATCTC AGATTTAGAA 
GAACGCTTGA AGAAGGAAGA GAAGACGCGT CAGGAGCTGG AGAAGGCCAA ACGAAAACTG 
GACGGTGAGA CCACTGACCT GCAGGACCAG ATCGCAGAGC TCCAGGCGCA GATCGACGAG 
CTCAAGGTCC AGCTGGCCAA GAAGGAGGAG GAGCTGCAAG GCGCGCTGGC CAGGGGTGAC 
GATGAGACGC TCCATAAGAA CAATGCCCTC AAGGTGGTGC GAGAGCTGCA AGCCCAAATC 
GCCGAGCTTC AGGAGGACTT TGAATCAGAG AAGGCTTCAC GGAACAAGGC GGAGAAGCAG 
AAGAGGGACC TGAGTGAGGA GCTGGAGGCC CTGAAAACAG AGCTGGAGGA CACGCTGGAC 
ACCACCGCGG CTCAGCAGGA ACTTCGCACG AAGCGTGAAC AAGAAGTGGC AGAGCTGAAG 
AAGGCTCTGG AGGAGGAAAC GAGGAGCCAC GAGGCTCAAA TCCAGGACAT GAGACAGCGA 
CACGCCACAG CCCTGGAGGA GCTGTCGGAG CAGCTGGAAC AGGCAAAGAG GTTCAAAGCG 
AATCTGGAGA AGAACAAGCA GGGCCTGGAG ACTGACAACA AGGAGCTGGC GTGTGAGGTG 
AAGGTGCTAC AGCAGGTCAA GGCTGAGTCT GAGCACAAGA GGAAGAAGCT CGACGCCCAG 
GTGCAGGAGC TGCACGCCAA GGTCTCAGAA GGAGACAGGC TCAGGGTGGA GCTGGCCGAG 
AAGGCAAACA AATTACAGAA TGAACTGGAC AATGTCTCAA GTCTTCTGGA AGAAGCAGAG 
AAGAAGGGCA TTAAGTTTGC TAAGGACGCA GCTAGTCTTG AGTCTCAACT ACAGGACACT 
CAGGAGCTCC TTCAGGAGGA GACCCGCCAG AAGCTGAACC TGAGCAGCCG CATCCGGCAG 
CTGGAGGAGG AGAAGAACAG TCTCCAGGAG CAGCAGGAGG AGGAGGAGGA GGCCAGGAAA 
AACCTGGAAA AGCAGGTGTT GGTGCTGCAG TCCCAGCTCG CCGATACCAA GAAGAAGGTA 
GATGATGACT TAGGAACCAT CGAGAGCTTA GAAGAAGCCA GAAAGAAGCT TCTGAAAGAT 
GTGGAGGCCC TGGGCCAGCG CCTGGAGGAG AAGGCTCTGG CCTATGACAA GCTGGAGAAG 
ACCAAGAACC GCCTGCAGCA GGAGCTCGAC GACCTGACAG TGGACCTAGA TCACCAGCGC 
CAGATCGCCT CCAACTTGGA GAAGAAGCAG AAGAAGTTTG ACCAGCTGCT GGCAGAGGAG 
AAGAACATCT CCGCCCGCTA TGCAGAAGAG CGAGACCGCG CAGAGGCAGA GGCCAGGGAG 
AAGGAAACCA AAGCCCTGTC CCTGGCCCGA GCCCTCGAGG AGGCCCTAGA AACCAAGGAA 
GAGTTTGAGA GACAGAACAA GCAGCTGCGG GCAGACATGG AGGACCTGAT AAGCTCCAAG 
GATGACGTGG GGAAGAACGT GCACGAGCTT GAAAAATCGA AGCGGGCCCT GGAGCAACAG 
GTGGAGGAGA TGAGGACACA GCTGGAAGAG CTGGAGGATG AGCTTCAGGC CACAGAAGAC 
GCCAAGCTTC GCCTGGAAGT CAACATGCAG GCCATGAAGG CCCAGTTTGA GAGGGACTTA 
CAAACCAGGG ACGAGCAGAA TGAAGAGAAG AAGCGGCTCC TGGTTAAGCA GGTGCGGGAG 
CTGGAGGCAG AGCTGGAAGA TGAGCGGAAG CAGCGAGCGC TGGCGGTCGC TTCTAAAAAG 
AAACTGGAAA TAGACCTGAA GGACCTTGAA GCCCAGATTG AGGCTGCGAA CAAAGCGAGG 
GACGAAGTGA TCAAGCAGCT TCGCAAGCTT CAGGCTCAGA TGAAGGATTA CCAACGTGAA 
TTAGAAGAAG CCCGTGCATC CCGGGATGAG ATTTTTGCTC AATCCAAAGA GAGTGAAAAG 
AAACTGAAGA GTCTGGAAGC AGAAATCCTT CAATTGCAGG AGGAACTGGC CTCATCTGAG 
CGAGCGCGCC GCCACGCCGA GCAGGAGAGA GATGAGCTGG CTGACGAGAT CGCCAACAGC 
GCCTCTGGCA AGTCTGCGCT GCTGGATGAG AAGCGGCGTC TGGAGGCACG GATCGCGCAG 
CTGGAGGAGG AGCTGGAAGA GGAGCAGAGC AACATGGAGC TGCTCAACGA CCGCTTCCGC 
AAGACCACGC TGCAGGTGGA CACGCTGAAC GCCGAGCTCG CGGCTGAGCG CAGCGCCGCC 
CAGAAGAGCG ACAACGCACG CCAGCAGCTA GAGCGGCAGA ACAAGGAGCT GAAGGCCAAG 
CTGCAGGAAC TGGAGGGCGC CGTCAAGTCC AAGTTCAAGG CCACCATCTC TGCCCTGGAA 
GCCAAGATTG GGCAGCTGGA GGAGCAGCTG GAGCAGGAAG CCAAGGAACG AGCAGCCGCC 
AACAAACTAG TCCGTCGCAC GGAGAAAAAG CTGAAAGAGA TCTTCATGCA GGTTGAAGAT 
GAGCGTCGAC ATGCGGATCA GTATAAAGAA CAGATGGAGA AGGCCAACGC CAGGATGAAA 
CAGCTCAAGC GGCAGCTGGA GGAGGCAGAG GAGGAAGCCA CACGTGCCAA CGCATCTCGG 
CGTAAGCTCC AGCGGGAACT GGACGATGCC ACGGAGGCCA ACGAGGGCCT GAGCCGTGAG 
GTCAGCACGC TGAAGAACCG GCTGAGACGA GGTGGCCCCA TAAGCTTCTC GTCCAGCCGA 
TCGGGCCGGC GCCAGCTACA CATCGAGGGA GCCTCCCTGG AGCTGTCAGA CGATGACACG 
GAGAGTAAGA CCAGTGACGT CAACGAGACA CAGCCGCCGC AGTCGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969074.1
CDS	132..6062
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_014113599.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113599.1

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC CTTTGGAAAT GCGAAGACTG TGAAAAATGA TAATTCATCT 
CGCTTTGGCA AGTTTATTCG AATCAACTTT GATGTAACTG GCTATATTGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAGTT GTTATCTGGA GCAGGAGAAC ACCTAAAGTC GGATTTGCTC 
CTCGAAGGAT TTAATAACTA CAGATTCCTC TCCAACGGCT ATATTCCCAT CCCGGGACAG 
CAAGACAAGG ACAACTTTCA GGAAACCATG GAAGCAATGC ACATCATGGG CTTCTCTCAC 
GAGGAGATTC TGTCAATGCT TAAAGTAGTG TCATCAGTGC TACAGTTCGG AAATATTTCT 
TTCAAAAAGG AGCGAAATAC TGATCAAGCA TCCATGCCAG AGAACACAGT TGCACAGAAA 
CTCTGCCATC TTCTTGGGAT GAACGTGATG GAGTTCACGC GGGCCATCCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA TGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCG 
GTAGAAGCAT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GTTGGCTCGT TCACCGTATC 
AACAAAGCTC TGGACAGGAC CAAGCGTCAG GGCGCATCTT TTATTGGAAT CCTGGATATC 
GCTGGATTCG AAATATTTGA GCTCAACTCG TTCGAGCAAC TGTGCATCAA CTACACCAAT 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAG CTTCATTGAC TTTGGCCTCG ACCTGCAGCC CTGCATCGAC 
CTCATCGAGA GACCCGCTAA CCCCCCTGGT GTGCTGGCCC TTTTGGATGA AGAGTGCTGG 
TTCCCGAAGG CCACGGACAA AACCTTTGTT GAAAAACTAG TTCAAGAGCA AGGCTCTCAC 
TCCAAGTTCC AGAAACCCCG GCAACTGAAA GACAGAGCTG ACTTCTGCAT CATCCATTAT 
GCGGGCAAGG TGGACTACAA GGCTGATGAG TGGCTGATGA AGAACATGGA CCCCCTGAAC 
GACAATGTGG CCACCCTCCT GCACCAGTCA TCTGACAGAT TCGTGGCAGA GCTTTGGAAG 
GATGTGGACC GTATCGTGGG TCTGGATCAG GTCACGGGCA TGACGGAGAC AGCCTTTGGC 
TCTGCGTATA AAACCAAGAA GGGCATGTTT CGGACTGTCG GGCAGCTCTA CAAAGAATCC 
CTCACCAAGC TGATGGCAAC TCTCCGGAAC ACCAACCCTA ACTTCGTCCG CTGCATCATT 
CCAAATCACG AGAAGCGAGC TGGAAAACTG GATCCGCACC TAGTGCTTGA TCAGCTTCGC 
TGTAACGGCG TTCTGGAAGG AATCAGGATC TGTCGCCAGG GGTTCCCCAA CCGGATAGTT 
TTCCAGGAAT TCAGACAGAG ATACGAGATC CTAACTCCAA ATGCTATCCC TAAGGGTTTC 
ATGGACGGCA AACAGGCCTG CGAACGAATG ATCCGGGCTT TAGAACTGGA CCCAAACTTG 
TACAGAATCG GACAGAGCAA GATATTTTTC CGAGCTGGCG TGCTGGCACA CTTAGAGGAA 
GAGAGGGATC TAAAGATCAC CGACATCATT ATCTTCTTCC AGGCTGTCTG CAGAGGCTAC 
CTGGCCAGAA AGGCCTTCGC CAAGAAGCAG CAGCAGCTGA GCGCCCTGAA GGTCCTGCAG 
CGGAACTGCG CCGCATACCT GAAGCTCCGG CACTGGCAGT GGTGGCGTGT CTTCACGAAG 
GTGAAGCCGC TCCTGCAAGT GACCCGCCAG GAGGAAGAGC TTCAGGCCAA AGACGAGGAG 
CTGCTGAAGG TGAAGGAGAG GCAGACGAAG GTGGAGGGCG AGCTGGAGGA AATGGAGAGG 
AAGCACCAGC AGCTCCTGGA AGAGAAGAAC ATCCTAACAG AGCAGCTCCA AGCAGAGACA 
GAGCTCTTTG CTGAGGCAGA AGAGATGAGA GCCAGGCTCG CCGCCAAGAA GCAGGAGCTG 
GAGGAGATTC TCCATGACTT GGAATCCAGG GTCGAAGAGG AAGAAGAACG AAACCAAATC 
CTCCAAAATG AAAAGAAGAA AATGCAAGCT CACATTCAGG ACCTAGAGGA GCAACTGGAT 
GAGGAAGAAG GCGCTCGCCA GAAACTACAG CTGGAAAAGG TGACAGCGGA AGCAAAAATC 
AAGAAGATGG AGGAGGAGAT CCTGCTGCTT GAGGACCAGA ATTCCAAATT TATCAAAGAA 
AAGAAACTCA TGGAAGATCG CATTGCAGAG TGTTCCTCTC AGCTGGCTGA GGAGGAAGAA 
AAGGCCAAAA ACTTGGCCAA AATCAGGAAT AAGCAGGAAG TGATGATCTC AGATTTAGAA 
GAACGCTTGA AGAAGGAAGA GAAGACGCGT CAGGAGCTGG AGAAGGCCAA ACGAAAACTG 
GACGGTGAGA CCACTGACCT GCAGGACCAG ATCGCAGAGC TCCAGGCGCA GATCGACGAG 
CTCAAGGTCC AGCTGGCCAA GAAGGAGGAG GAGCTGCAAG GCGCGCTGGC CAGGGGTGAC 
GATGAGACGC TCCATAAGAA CAATGCCCTC AAGGTGGTGC GAGAGCTGCA AGCCCAAATC 
GCCGAGCTTC AGGAGGACTT TGAATCAGAG AAGGCTTCAC GGAACAAGGC GGAGAAGCAG 
AAGAGGGACC TGAGTGAGGA GCTGGAGGCC CTGAAAACAG AGCTGGAGGA CACGCTGGAC 
ACCACCGCGG CTCAGCAGGA ACTTCGCACG AAGCGTGAAC AAGAAGTGGC AGAGCTGAAG 
AAGGCTCTGG AGGAGGAAAC GAGGAGCCAC GAGGCTCAAA TCCAGGACAT GAGACAGCGA 
CACGCCACAG CCCTGGAGGA GCTGTCGGAG CAGCTGGAAC AGGCAAAGAG GTTCAAAGCG 
AATCTGGAGA AGAACAAGCA GGGCCTGGAG ACTGACAACA AGGAGCTGGC GTGTGAGGTG 
AAGGTGCTAC AGCAGGTCAA GGCTGAGTCT GAGCACAAGA GGAAGAAGCT CGACGCCCAG 
GTGCAGGAGC TGCACGCCAA GGTCTCAGAA GGAGACAGGC TCAGGGTGGA GCTGGCCGAG 
AAGGCAAACA AATTACAGAA TGAACTGGAC AATGTCTCAA GTCTTCTGGA AGAAGCAGAG 
AAGAAGGGCA TTAAGTTTGC TAAGGACGCA GCTAGTCTTG AGTCTCAACT ACAGGACACT 
CAGGAGCTCC TTCAGGAGGA GACCCGCCAG AAGCTGAACC TGAGCAGCCG CATCCGGCAG 
CTGGAGGAGG AGAAGAACAG TCTCCAGGAG CAGCAGGAGG AGGAGGAGGA GGCCAGGAAA 
AACCTGGAAA AGCAGGTGTT GGTGCTGCAG TCCCAGCTCG CCGATACCAA GAAGAAGGTA 
GATGATGACT TAGGAACCAT CGAGAGCTTA GAAGAAGCCA GAAAGAAGCT TCTGAAAGAT 
GTGGAGGCCC TGGGCCAGCG CCTGGAGGAG AAGGCTCTGG CCTATGACAA GCTGGAGAAG 
ACCAAGAACC GCCTGCAGCA GGAGCTCGAC GACCTGACAG TGGACCTAGA TCACCAGCGC 
CAGATCGCCT CCAACTTGGA GAAGAAGCAG AAGAAGTTTG ACCAGCTGCT GGCAGAGGAG 
AAGAACATCT CCGCCCGCTA TGCAGAAGAG CGAGACCGCG CAGAGGCAGA GGCCAGGGAG 
AAGGAAACCA AAGCCCTGTC CCTGGCCCGA GCCCTCGAGG AGGCCCTAGA AACCAAGGAA 
GAGTTTGAGA GACAGAACAA GCAGCTGCGG GCAGACATGG AGGACCTGAT AAGCTCCAAG 
GATGACGTGG GGAAGAACGT GCACGAGCTT GAAAAATCGA AGCGGGCCCT GGAGCAACAG 
GTGGAGGAGA TGAGGACACA GCTGGAAGAG CTGGAGGATG AGCTTCAGGC CACAGAAGAC 
GCCAAGCTTC GCCTGGAAGT CAACATGCAG GCCATGAAGG CCCAGTTTGA GAGGGACTTA 
CAAACCAGGG ACGAGCAGAA TGAAGAGAAG AAGCGGCTCC TGGTTAAGCA GGTGCGGGAG 
CTGGAGGCAG AGCTGGAAGA TGAGCGGAAG CAGCGAGCGC TGGCGGTCGC TTCTAAAAAG 
AAACTGGAAA TAGACCTGAA GGACCTTGAA GCCCAGATTG AGGCTGCGAA CAAAGCGAGG 
GACGAAGTGA TCAAGCAGCT TCGCAAGCTT CAGGCTCAGA TGAAGGATTA CCAACGTGAA 
TTAGAAGAAG CCCGTGCATC CCGGGATGAG ATTTTTGCTC AATCCAAAGA GAGTGAAAAG 
AAACTGAAGA GTCTGGAAGC AGAAATCCTT CAATTGCAGG AGGAACTGGC CTCATCTGAG 
CGAGCGCGCC GCCACGCCGA GCAGGAGAGA GATGAGCTGG CTGACGAGAT CGCCAACAGC 
GCCTCTGGCA AGTCTGCGCT GCTGGATGAG AAGCGGCGTC TGGAGGCACG GATCGCGCAG 
CTGGAGGAGG AGCTGGAAGA GGAGCAGAGC AACATGGAGC TGCTCAACGA CCGCTTCCGC 
AAGACCACGC TGCAGGTGGA CACGCTGAAC GCCGAGCTCG CGGCTGAGCG CAGCGCCGCC 
CAGAAGAGCG ACAACGCACG CCAGCAGCTA GAGCGGCAGA ACAAGGAGCT GAAGGCCAAG 
CTGCAGGAAC TGGAGGGCGC CGTCAAGTCC AAGTTCAAGG CCACCATCTC TGCCCTGGAA 
GCCAAGATTG GGCAGCTGGA GGAGCAGCTG GAGCAGGAAG CCAAGGAACG AGCAGCCGCC 
AACAAACTAG TCCGTCGCAC GGAGAAAAAG CTGAAAGAGA TCTTCATGCA GGTTGAAGAT 
GAGCGTCGAC ATGCGGATCA GTATAAAGAA CAGATGGAGA AGGCCAACGC CAGGATGAAA 
CAGCTCAAGC GGCAGCTGGA GGAGGCAGAG GAGGAAGCCA CACGTGCCAA CGCATCTCGG 
CGTAAGCTCC AGCGGGAACT GGACGATGCC ACGGAGGCCA ACGAGGGCCT GAGCCGTGAG 
GTCAGCACGC TGAAGAACCG GCTGAGACGA GGTGGCCCCA TAAGCTTCTC GTCCAGCCGA 
TCGGGCCGGC GCCAGCTACA CATCGAGGGA GCCTCCCTGG AGCTGTCAGA CGATGACACG 
GAGAGTAAGA CCAGTGACGT CAACGAGACA CAGCCGCCGC AGTCGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_014113599.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969074.1 Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X6, mRNA.

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CloneID	OCe36461
Clone ID Related Accession (Same CDS sequence)	XM_014113599.1 , XM_014113599.2
Accession Version	XM_014113599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	myosin-10 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113599.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC CTTTGGAAAT GCGAAGACTG TGAAAAATGA TAATTCATCT 
CGCTTTGGCA AGTTTATTCG AATCAACTTT GATGTAACTG GCTATATTGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAGTT GTTATCTGGA GCAGGAGAAC ACCTAAAGTC GGATTTGCTC 
CTCGAAGGAT TTAATAACTA CAGATTCCTC TCCAACGGCT ATATTCCCAT CCCGGGACAG 
CAAGACAAGG ACAACTTTCA GGAAACCATG GAAGCAATGC ACATCATGGG CTTCTCTCAC 
GAGGAGATTC TGTCAATGCT TAAAGTAGTG TCATCAGTGC TACAGTTCGG AAATATTTCT 
TTCAAAAAGG AGCGAAATAC TGATCAAGCA TCCATGCCAG AGAACACAGT TGCACAGAAA 
CTCTGCCATC TTCTTGGGAT GAACGTGATG GAGTTCACGC GGGCCATCCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA TGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCG 
GTAGAAGCAT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GTTGGCTCGT TCACCGTATC 
AACAAAGCTC TGGACAGGAC CAAGCGTCAG GGCGCATCTT TTATTGGAAT CCTGGATATC 
GCTGGATTCG AAATATTTGA GCTCAACTCG TTCGAGCAAC TGTGCATCAA CTACACCAAT 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAG CTTCATTGAC TTTGGCCTCG ACCTGCAGCC CTGCATCGAC 
CTCATCGAGA GACCCGCTAA CCCCCCTGGT GTGCTGGCCC TTTTGGATGA AGAGTGCTGG 
TTCCCGAAGG CCACGGACAA AACCTTTGTT GAAAAACTAG TTCAAGAGCA AGGCTCTCAC 
TCCAAGTTCC AGAAACCCCG GCAACTGAAA GACAGAGCTG ACTTCTGCAT CATCCATTAT 
GCGGGCAAGG TGGACTACAA GGCTGATGAG TGGCTGATGA AGAACATGGA CCCCCTGAAC 
GACAATGTGG CCACCCTCCT GCACCAGTCA TCTGACAGAT TCGTGGCAGA GCTTTGGAAG 
GATGTGGACC GTATCGTGGG TCTGGATCAG GTCACGGGCA TGACGGAGAC AGCCTTTGGC 
TCTGCGTATA AAACCAAGAA GGGCATGTTT CGGACTGTCG GGCAGCTCTA CAAAGAATCC 
CTCACCAAGC TGATGGCAAC TCTCCGGAAC ACCAACCCTA ACTTCGTCCG CTGCATCATT 
CCAAATCACG AGAAGCGAGC TGGAAAACTG GATCCGCACC TAGTGCTTGA TCAGCTTCGC 
TGTAACGGCG TTCTGGAAGG AATCAGGATC TGTCGCCAGG GGTTCCCCAA CCGGATAGTT 
TTCCAGGAAT TCAGACAGAG ATACGAGATC CTAACTCCAA ATGCTATCCC TAAGGGTTTC 
ATGGACGGCA AACAGGCCTG CGAACGAATG ATCCGGGCTT TAGAACTGGA CCCAAACTTG 
TACAGAATCG GACAGAGCAA GATATTTTTC CGAGCTGGCG TGCTGGCACA CTTAGAGGAA 
GAGAGGGATC TAAAGATCAC CGACATCATT ATCTTCTTCC AGGCTGTCTG CAGAGGCTAC 
CTGGCCAGAA AGGCCTTCGC CAAGAAGCAG CAGCAGCTGA GCGCCCTGAA GGTCCTGCAG 
CGGAACTGCG CCGCATACCT GAAGCTCCGG CACTGGCAGT GGTGGCGTGT CTTCACGAAG 
GTGAAGCCGC TCCTGCAAGT GACCCGCCAG GAGGAAGAGC TTCAGGCCAA AGACGAGGAG 
CTGCTGAAGG TGAAGGAGAG GCAGACGAAG GTGGAGGGCG AGCTGGAGGA AATGGAGAGG 
AAGCACCAGC AGCTCCTGGA AGAGAAGAAC ATCCTAACAG AGCAGCTCCA AGCAGAGACA 
GAGCTCTTTG CTGAGGCAGA AGAGATGAGA GCCAGGCTCG CCGCCAAGAA GCAGGAGCTG 
GAGGAGATTC TCCATGACTT GGAATCCAGG GTCGAAGAGG AAGAAGAACG AAACCAAATC 
CTCCAAAATG AAAAGAAGAA AATGCAAGCT CACATTCAGG ACCTAGAGGA GCAACTGGAT 
GAGGAAGAAG GCGCTCGCCA GAAACTACAG CTGGAAAAGG TGACAGCGGA AGCAAAAATC 
AAGAAGATGG AGGAGGAGAT CCTGCTGCTT GAGGACCAGA ATTCCAAATT TATCAAAGAA 
AAGAAACTCA TGGAAGATCG CATTGCAGAG TGTTCCTCTC AGCTGGCTGA GGAGGAAGAA 
AAGGCCAAAA ACTTGGCCAA AATCAGGAAT AAGCAGGAAG TGATGATCTC AGATTTAGAA 
GAACGCTTGA AGAAGGAAGA GAAGACGCGT CAGGAGCTGG AGAAGGCCAA ACGAAAACTG 
GACGGTGAGA CCACTGACCT GCAGGACCAG ATCGCAGAGC TCCAGGCGCA GATCGACGAG 
CTCAAGGTCC AGCTGGCCAA GAAGGAGGAG GAGCTGCAAG GCGCGCTGGC CAGGGGTGAC 
GATGAGACGC TCCATAAGAA CAATGCCCTC AAGGTGGTGC GAGAGCTGCA AGCCCAAATC 
GCCGAGCTTC AGGAGGACTT TGAATCAGAG AAGGCTTCAC GGAACAAGGC GGAGAAGCAG 
AAGAGGGACC TGAGTGAGGA GCTGGAGGCC CTGAAAACAG AGCTGGAGGA CACGCTGGAC 
ACCACCGCGG CTCAGCAGGA ACTTCGCACG AAGCGTGAAC AAGAAGTGGC AGAGCTGAAG 
AAGGCTCTGG AGGAGGAAAC GAGGAGCCAC GAGGCTCAAA TCCAGGACAT GAGACAGCGA 
CACGCCACAG CCCTGGAGGA GCTGTCGGAG CAGCTGGAAC AGGCAAAGAG GTTCAAAGCG 
AATCTGGAGA AGAACAAGCA GGGCCTGGAG ACTGACAACA AGGAGCTGGC GTGTGAGGTG 
AAGGTGCTAC AGCAGGTCAA GGCTGAGTCT GAGCACAAGA GGAAGAAGCT CGACGCCCAG 
GTGCAGGAGC TGCACGCCAA GGTCTCAGAA GGAGACAGGC TCAGGGTGGA GCTGGCCGAG 
AAGGCAAACA AATTACAGAA TGAACTGGAC AATGTCTCAA GTCTTCTGGA AGAAGCAGAG 
AAGAAGGGCA TTAAGTTTGC TAAGGACGCA GCTAGTCTTG AGTCTCAACT ACAGGACACT 
CAGGAGCTCC TTCAGGAGGA GACCCGCCAG AAGCTGAACC TGAGCAGCCG CATCCGGCAG 
CTGGAGGAGG AGAAGAACAG TCTCCAGGAG CAGCAGGAGG AGGAGGAGGA GGCCAGGAAA 
AACCTGGAAA AGCAGGTGTT GGTGCTGCAG TCCCAGCTCG CCGATACCAA GAAGAAGGTA 
GATGATGACT TAGGAACCAT CGAGAGCTTA GAAGAAGCCA GAAAGAAGCT TCTGAAAGAT 
GTGGAGGCCC TGGGCCAGCG CCTGGAGGAG AAGGCTCTGG CCTATGACAA GCTGGAGAAG 
ACCAAGAACC GCCTGCAGCA GGAGCTCGAC GACCTGACAG TGGACCTAGA TCACCAGCGC 
CAGATCGCCT CCAACTTGGA GAAGAAGCAG AAGAAGTTTG ACCAGCTGCT GGCAGAGGAG 
AAGAACATCT CCGCCCGCTA TGCAGAAGAG CGAGACCGCG CAGAGGCAGA GGCCAGGGAG 
AAGGAAACCA AAGCCCTGTC CCTGGCCCGA GCCCTCGAGG AGGCCCTAGA AACCAAGGAA 
GAGTTTGAGA GACAGAACAA GCAGCTGCGG GCAGACATGG AGGACCTGAT AAGCTCCAAG 
GATGACGTGG GGAAGAACGT GCACGAGCTT GAAAAATCGA AGCGGGCCCT GGAGCAACAG 
GTGGAGGAGA TGAGGACACA GCTGGAAGAG CTGGAGGATG AGCTTCAGGC CACAGAAGAC 
GCCAAGCTTC GCCTGGAAGT CAACATGCAG GCCATGAAGG CCCAGTTTGA GAGGGACTTA 
CAAACCAGGG ACGAGCAGAA TGAAGAGAAG AAGCGGCTCC TGGTTAAGCA GGTGCGGGAG 
CTGGAGGCAG AGCTGGAAGA TGAGCGGAAG CAGCGAGCGC TGGCGGTCGC TTCTAAAAAG 
AAACTGGAAA TAGACCTGAA GGACCTTGAA GCCCAGATTG AGGCTGCGAA CAAAGCGAGG 
GACGAAGTGA TCAAGCAGCT TCGCAAGCTT CAGGCTCAGA TGAAGGATTA CCAACGTGAA 
TTAGAAGAAG CCCGTGCATC CCGGGATGAG ATTTTTGCTC AATCCAAAGA GAGTGAAAAG 
AAACTGAAGA GTCTGGAAGC AGAAATCCTT CAATTGCAGG AGGAACTGGC CTCATCTGAG 
CGAGCGCGCC GCCACGCCGA GCAGGAGAGA GATGAGCTGG CTGACGAGAT CGCCAACAGC 
GCCTCTGGCA AGTCTGCGCT GCTGGATGAG AAGCGGCGTC TGGAGGCACG GATCGCGCAG 
CTGGAGGAGG AGCTGGAAGA GGAGCAGAGC AACATGGAGC TGCTCAACGA CCGCTTCCGC 
AAGACCACGC TGCAGGTGGA CACGCTGAAC GCCGAGCTCG CGGCTGAGCG CAGCGCCGCC 
CAGAAGAGCG ACAACGCACG CCAGCAGCTA GAGCGGCAGA ACAAGGAGCT GAAGGCCAAG 
CTGCAGGAAC TGGAGGGCGC CGTCAAGTCC AAGTTCAAGG CCACCATCTC TGCCCTGGAA 
GCCAAGATTG GGCAGCTGGA GGAGCAGCTG GAGCAGGAAG CCAAGGAACG AGCAGCCGCC 
AACAAACTAG TCCGTCGCAC GGAGAAAAAG CTGAAAGAGA TCTTCATGCA GGTTGAAGAT 
GAGCGTCGAC ATGCGGATCA GTATAAAGAA CAGATGGAGA AGGCCAACGC CAGGATGAAA 
CAGCTCAAGC GGCAGCTGGA GGAGGCAGAG GAGGAAGCCA CACGTGCCAA CGCATCTCGG 
CGTAAGCTCC AGCGGGAACT GGACGATGCC ACGGAGGCCA ACGAGGGCCT GAGCCGTGAG 
GTCAGCACGC TGAAGAACCG GCTGAGACGA GGTGGCCCCA TAAGCTTCTC GTCCAGCCGA 
TCGGGCCGGC GCCAGCTACA CATCGAGGGA GCCTCCCTGG AGCTGTCAGA CGATGACACG 
GAGAGTAAGA CCAGTGACGT CAACGAGACA CAGCCGCCGC AGTCGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969074.1
CDS	192..6122
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_014113599.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113599.2

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC CTTTGGAAAT GCGAAGACTG TGAAAAATGA TAATTCATCT 
CGCTTTGGCA AGTTTATTCG AATCAACTTT GATGTAACTG GCTATATTGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAGTT GTTATCTGGA GCAGGAGAAC ACCTAAAGTC GGATTTGCTC 
CTCGAAGGAT TTAATAACTA CAGATTCCTC TCCAACGGCT ATATTCCCAT CCCGGGACAG 
CAAGACAAGG ACAACTTTCA GGAAACCATG GAAGCAATGC ACATCATGGG CTTCTCTCAC 
GAGGAGATTC TGTCAATGCT TAAAGTAGTG TCATCAGTGC TACAGTTCGG AAATATTTCT 
TTCAAAAAGG AGCGAAATAC TGATCAAGCA TCCATGCCAG AGAACACAGT TGCACAGAAA 
CTCTGCCATC TTCTTGGGAT GAACGTGATG GAGTTCACGC GGGCCATCCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA TGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCG 
GTAGAAGCAT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GTTGGCTCGT TCACCGTATC 
AACAAAGCTC TGGACAGGAC CAAGCGTCAG GGCGCATCTT TTATTGGAAT CCTGGATATC 
GCTGGATTCG AAATATTTGA GCTCAACTCG TTCGAGCAAC TGTGCATCAA CTACACCAAT 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAG CTTCATTGAC TTTGGCCTCG ACCTGCAGCC CTGCATCGAC 
CTCATCGAGA GACCCGCTAA CCCCCCTGGT GTGCTGGCCC TTTTGGATGA AGAGTGCTGG 
TTCCCGAAGG CCACGGACAA AACCTTTGTT GAAAAACTAG TTCAAGAGCA AGGCTCTCAC 
TCCAAGTTCC AGAAACCCCG GCAACTGAAA GACAGAGCTG ACTTCTGCAT CATCCATTAT 
GCGGGCAAGG TGGACTACAA GGCTGATGAG TGGCTGATGA AGAACATGGA CCCCCTGAAC 
GACAATGTGG CCACCCTCCT GCACCAGTCA TCTGACAGAT TCGTGGCAGA GCTTTGGAAG 
GATGTGGACC GTATCGTGGG TCTGGATCAG GTCACGGGCA TGACGGAGAC AGCCTTTGGC 
TCTGCGTATA AAACCAAGAA GGGCATGTTT CGGACTGTCG GGCAGCTCTA CAAAGAATCC 
CTCACCAAGC TGATGGCAAC TCTCCGGAAC ACCAACCCTA ACTTCGTCCG CTGCATCATT 
CCAAATCACG AGAAGCGAGC TGGAAAACTG GATCCGCACC TAGTGCTTGA TCAGCTTCGC 
TGTAACGGCG TTCTGGAAGG AATCAGGATC TGTCGCCAGG GGTTCCCCAA CCGGATAGTT 
TTCCAGGAAT TCAGACAGAG ATACGAGATC CTAACTCCAA ATGCTATCCC TAAGGGTTTC 
ATGGACGGCA AACAGGCCTG CGAACGAATG ATCCGGGCTT TAGAACTGGA CCCAAACTTG 
TACAGAATCG GACAGAGCAA GATATTTTTC CGAGCTGGCG TGCTGGCACA CTTAGAGGAA 
GAGAGGGATC TAAAGATCAC CGACATCATT ATCTTCTTCC AGGCTGTCTG CAGAGGCTAC 
CTGGCCAGAA AGGCCTTCGC CAAGAAGCAG CAGCAGCTGA GCGCCCTGAA GGTCCTGCAG 
CGGAACTGCG CCGCATACCT GAAGCTCCGG CACTGGCAGT GGTGGCGTGT CTTCACGAAG 
GTGAAGCCGC TCCTGCAAGT GACCCGCCAG GAGGAAGAGC TTCAGGCCAA AGACGAGGAG 
CTGCTGAAGG TGAAGGAGAG GCAGACGAAG GTGGAGGGCG AGCTGGAGGA AATGGAGAGG 
AAGCACCAGC AGCTCCTGGA AGAGAAGAAC ATCCTAACAG AGCAGCTCCA AGCAGAGACA 
GAGCTCTTTG CTGAGGCAGA AGAGATGAGA GCCAGGCTCG CCGCCAAGAA GCAGGAGCTG 
GAGGAGATTC TCCATGACTT GGAATCCAGG GTCGAAGAGG AAGAAGAACG AAACCAAATC 
CTCCAAAATG AAAAGAAGAA AATGCAAGCT CACATTCAGG ACCTAGAGGA GCAACTGGAT 
GAGGAAGAAG GCGCTCGCCA GAAACTACAG CTGGAAAAGG TGACAGCGGA AGCAAAAATC 
AAGAAGATGG AGGAGGAGAT CCTGCTGCTT GAGGACCAGA ATTCCAAATT TATCAAAGAA 
AAGAAACTCA TGGAAGATCG CATTGCAGAG TGTTCCTCTC AGCTGGCTGA GGAGGAAGAA 
AAGGCCAAAA ACTTGGCCAA AATCAGGAAT AAGCAGGAAG TGATGATCTC AGATTTAGAA 
GAACGCTTGA AGAAGGAAGA GAAGACGCGT CAGGAGCTGG AGAAGGCCAA ACGAAAACTG 
GACGGTGAGA CCACTGACCT GCAGGACCAG ATCGCAGAGC TCCAGGCGCA GATCGACGAG 
CTCAAGGTCC AGCTGGCCAA GAAGGAGGAG GAGCTGCAAG GCGCGCTGGC CAGGGGTGAC 
GATGAGACGC TCCATAAGAA CAATGCCCTC AAGGTGGTGC GAGAGCTGCA AGCCCAAATC 
GCCGAGCTTC AGGAGGACTT TGAATCAGAG AAGGCTTCAC GGAACAAGGC GGAGAAGCAG 
AAGAGGGACC TGAGTGAGGA GCTGGAGGCC CTGAAAACAG AGCTGGAGGA CACGCTGGAC 
ACCACCGCGG CTCAGCAGGA ACTTCGCACG AAGCGTGAAC AAGAAGTGGC AGAGCTGAAG 
AAGGCTCTGG AGGAGGAAAC GAGGAGCCAC GAGGCTCAAA TCCAGGACAT GAGACAGCGA 
CACGCCACAG CCCTGGAGGA GCTGTCGGAG CAGCTGGAAC AGGCAAAGAG GTTCAAAGCG 
AATCTGGAGA AGAACAAGCA GGGCCTGGAG ACTGACAACA AGGAGCTGGC GTGTGAGGTG 
AAGGTGCTAC AGCAGGTCAA GGCTGAGTCT GAGCACAAGA GGAAGAAGCT CGACGCCCAG 
GTGCAGGAGC TGCACGCCAA GGTCTCAGAA GGAGACAGGC TCAGGGTGGA GCTGGCCGAG 
AAGGCAAACA AATTACAGAA TGAACTGGAC AATGTCTCAA GTCTTCTGGA AGAAGCAGAG 
AAGAAGGGCA TTAAGTTTGC TAAGGACGCA GCTAGTCTTG AGTCTCAACT ACAGGACACT 
CAGGAGCTCC TTCAGGAGGA GACCCGCCAG AAGCTGAACC TGAGCAGCCG CATCCGGCAG 
CTGGAGGAGG AGAAGAACAG TCTCCAGGAG CAGCAGGAGG AGGAGGAGGA GGCCAGGAAA 
AACCTGGAAA AGCAGGTGTT GGTGCTGCAG TCCCAGCTCG CCGATACCAA GAAGAAGGTA 
GATGATGACT TAGGAACCAT CGAGAGCTTA GAAGAAGCCA GAAAGAAGCT TCTGAAAGAT 
GTGGAGGCCC TGGGCCAGCG CCTGGAGGAG AAGGCTCTGG CCTATGACAA GCTGGAGAAG 
ACCAAGAACC GCCTGCAGCA GGAGCTCGAC GACCTGACAG TGGACCTAGA TCACCAGCGC 
CAGATCGCCT CCAACTTGGA GAAGAAGCAG AAGAAGTTTG ACCAGCTGCT GGCAGAGGAG 
AAGAACATCT CCGCCCGCTA TGCAGAAGAG CGAGACCGCG CAGAGGCAGA GGCCAGGGAG 
AAGGAAACCA AAGCCCTGTC CCTGGCCCGA GCCCTCGAGG AGGCCCTAGA AACCAAGGAA 
GAGTTTGAGA GACAGAACAA GCAGCTGCGG GCAGACATGG AGGACCTGAT AAGCTCCAAG 
GATGACGTGG GGAAGAACGT GCACGAGCTT GAAAAATCGA AGCGGGCCCT GGAGCAACAG 
GTGGAGGAGA TGAGGACACA GCTGGAAGAG CTGGAGGATG AGCTTCAGGC CACAGAAGAC 
GCCAAGCTTC GCCTGGAAGT CAACATGCAG GCCATGAAGG CCCAGTTTGA GAGGGACTTA 
CAAACCAGGG ACGAGCAGAA TGAAGAGAAG AAGCGGCTCC TGGTTAAGCA GGTGCGGGAG 
CTGGAGGCAG AGCTGGAAGA TGAGCGGAAG CAGCGAGCGC TGGCGGTCGC TTCTAAAAAG 
AAACTGGAAA TAGACCTGAA GGACCTTGAA GCCCAGATTG AGGCTGCGAA CAAAGCGAGG 
GACGAAGTGA TCAAGCAGCT TCGCAAGCTT CAGGCTCAGA TGAAGGATTA CCAACGTGAA 
TTAGAAGAAG CCCGTGCATC CCGGGATGAG ATTTTTGCTC AATCCAAAGA GAGTGAAAAG 
AAACTGAAGA GTCTGGAAGC AGAAATCCTT CAATTGCAGG AGGAACTGGC CTCATCTGAG 
CGAGCGCGCC GCCACGCCGA GCAGGAGAGA GATGAGCTGG CTGACGAGAT CGCCAACAGC 
GCCTCTGGCA AGTCTGCGCT GCTGGATGAG AAGCGGCGTC TGGAGGCACG GATCGCGCAG 
CTGGAGGAGG AGCTGGAAGA GGAGCAGAGC AACATGGAGC TGCTCAACGA CCGCTTCCGC 
AAGACCACGC TGCAGGTGGA CACGCTGAAC GCCGAGCTCG CGGCTGAGCG CAGCGCCGCC 
CAGAAGAGCG ACAACGCACG CCAGCAGCTA GAGCGGCAGA ACAAGGAGCT GAAGGCCAAG 
CTGCAGGAAC TGGAGGGCGC CGTCAAGTCC AAGTTCAAGG CCACCATCTC TGCCCTGGAA 
GCCAAGATTG GGCAGCTGGA GGAGCAGCTG GAGCAGGAAG CCAAGGAACG AGCAGCCGCC 
AACAAACTAG TCCGTCGCAC GGAGAAAAAG CTGAAAGAGA TCTTCATGCA GGTTGAAGAT 
GAGCGTCGAC ATGCGGATCA GTATAAAGAA CAGATGGAGA AGGCCAACGC CAGGATGAAA 
CAGCTCAAGC GGCAGCTGGA GGAGGCAGAG GAGGAAGCCA CACGTGCCAA CGCATCTCGG 
CGTAAGCTCC AGCGGGAACT GGACGATGCC ACGGAGGCCA ACGAGGGCCT GAGCCGTGAG 
GTCAGCACGC TGAAGAACCG GCTGAGACGA GGTGGCCCCA TAAGCTTCTC GTCCAGCCGA 
TCGGGCCGGC GCCAGCTACA CATCGAGGGA GCCTCCCTGG AGCTGTCAGA CGATGACACG 
GAGAGTAAGA CCAGTGACGT CAACGAGACA CAGCCGCCGC AGTCGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_005620039.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620096.1 Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X7, mRNA.

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CloneID	OCe06305
Clone ID Related Accession (Same CDS sequence)	XM_005620039.2
Accession Version	XM_005620039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	myosin-10 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACGGG CGCCCCGGCG GCCTGGGCCG GGAGCGGGAG CCAATGAGCG TCCTTCCAGA 
GGCAATTGCC CTTGGATCCT CCTGTTTACA ATGGCCCAGA GAACTGGACT TGAAGACCCA 
GAGAGGTACC TCTTTGTGGA CAGGGCTGTC ATCTATAACC CTGCCACCCA GGCCGACTGG 
ACGGCTAAAA AGCTAGTGTG GATTCCATCA GAGCGCCATG GTTTCGAGGC AGCAAGTATC 
AAAGAGGAGC GGGGAGATGA GGTTATGGTG GAGTTGGCAG AGAACGGCAA GAAAGCCATG 
GTCAACAAAG ACGATATTCA GAAGATGAAC CCACCTAAGT TTTCCAAGGT GGAGGATATG 
GCGGAATTGA CGTGCTTGAA CGAAGCTTCT GTTCTACATA ACCTGAAGGA CCGCTACTAT 
TCGGGATTGA TCTATACTTA CTCTGGACTC TTCTGTGTAG TCATAAATCC TTACAAGAAT 
CTTCCAATTT ACTCTGAGAA TATTATTGAA ATGTACAGAG GGAAGAAACG CCACGAGATG 
CCTCCACACA TCTACGCTAT ATCTGAATCT GCTTACAGAT GCATGCTTCA AGATCGTGAG 
GACCAGTCCA TTCTTTGCAC GGGCGAATCA GGTGCTGGGA AGACAGAAAA TACCAAGAAA 
GTCATTCAGT ACCTTGCTCA TGTTGCTTCT TCGCATAAAG GACGGAAGGA CCATAATATT 
CCTCAGGAAT CGCCTAAACC AGTGAAACAT CAGGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC CTTTGGAAAT GCGAAGACTG TGAAAAATGA TAATTCATCT 
CGCTTTGGCA AGTTTATTCG AATCAACTTT GATGTAACTG GCTATATTGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAGTT GTTATCTGGA GCAGGAGAAC ACCTAAAGTC GGATTTGCTC 
CTCGAAGGAT TTAATAACTA CAGATTCCTC TCCAACGGCT ATATTCCCAT CCCGGGACAG 
CAAGACAAGG ACAACTTTCA GGAAACCATG GAAGCAATGC ACATCATGGG CTTCTCTCAC 
GAGGAGATTC TGTCAATGCT TAAAGTAGTG TCATCAGTGC TACAGTTCGG AAATATTTCT 
TTCAAAAAGG AGCGAAATAC TGATCAAGCA TCCATGCCAG AGAACACAGT TGCACAGAAA 
CTCTGCCATC TTCTTGGGAT GAACGTGATG GAGTTCACGC GGGCCATCCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA TGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCG 
GTAGAAGCAT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GTTGGCTCGT TCACCGTATC 
AACAAAGCTC TGGACAGGAC CAAGCGTCAG GGCGCATCTT TTATTGGAAT CCTGGATATC 
GCTGGATTCG AAATATTTGA GCTCAACTCG TTCGAGCAAC TGTGCATCAA CTACACCAAT 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAG CTTCATTGAC TTTGGCCTCG ACCTGCAGCC CTGCATCGAC 
CTCATCGAGA GACCCGCTAA CCCCCCTGGT GTGCTGGCCC TTTTGGATGA AGAGTGCTGG 
TTCCCGAAGG CCACGGACAA AACCTTTGTT GAAAAACTAG TTCAAGAGCA AGGCTCTCAC 
TCCAAGTTCC AGAAACCCCG GCAACTGAAA GACAGAGCTG ACTTCTGCAT CATCCATTAT 
GCGGGCAAGG TGGACTACAA GGCTGATGAG TGGCTGATGA AGAACATGGA CCCCCTGAAC 
GACAATGTGG CCACCCTCCT GCACCAGTCA TCTGACAGAT TCGTGGCAGA GCTTTGGAAG 
GATGAGATTC AGAATATTCA GAGAGCTTCT TTCTATGACA GTGTTTCTGG TCTTCATGAG 
CCACCAGTGG ACCGTATCGT GGGTCTGGAT CAGGTCACGG GCATGACGGA GACAGCCTTT 
GGCTCTGCGT ATAAAACCAA GAAGGGCATG TTTCGGACTG TCGGGCAGCT CTACAAAGAA 
TCCCTCACCA AGCTGATGGC AACTCTCCGG AACACCAACC CTAACTTCGT CCGCTGCATC 
ATTCCAAATC ACGAGAAGCG AGCTGGAAAA CTGGATCCGC ACCTAGTGCT TGATCAGCTT 
CGCTGTAACG GCGTTCTGGA AGGAATCAGG ATCTGTCGCC AGGGGTTCCC CAACCGGATA 
GTTTTCCAGG AATTCAGACA GAGATACGAG ATCCTAACTC CAAATGCTAT CCCTAAGGGT 
TTCATGGACG GCAAACAGGC CTGCGAACGA ATGATCCGGG CTTTAGAACT GGACCCAAAC 
TTGTACAGAA TCGGACAGAG CAAGATATTT TTCCGAGCTG GCGTGCTGGC ACACTTAGAG 
GAAGAGAGGG ATCTAAAGAT CACCGACATC ATTATCTTCT TCCAGGCTGT CTGCAGAGGC 
TACCTGGCCA GAAAGGCCTT CGCCAAGAAG CAGCAGCAGC TGAGCGCCCT GAAGGTCCTG 
CAGCGGAACT GCGCCGCATA CCTGAAGCTC CGGCACTGGC AGTGGTGGCG TGTCTTCACG 
AAGGTGAAGC CGCTCCTGCA AGTGACCCGC CAGGAGGAAG AGCTTCAGGC CAAAGACGAG 
GAGCTGCTGA AGGTGAAGGA GAGGCAGACG AAGGTGGAGG GCGAGCTGGA GGAAATGGAG 
AGGAAGCACC AGCAGCTCCT GGAAGAGAAG AACATCCTAA CAGAGCAGCT CCAAGCAGAG 
ACAGAGCTCT TTGCTGAGGC AGAAGAGATG AGAGCCAGGC TCGCCGCCAA GAAGCAGGAG 
CTGGAGGAGA TTCTCCATGA CTTGGAATCC AGGGTCGAAG AGGAAGAAGA ACGAAACCAA 
ATCCTCCAAA ATGAAAAGAA GAAAATGCAA GCTCACATTC AGGACCTAGA GGAGCAACTG 
GATGAGGAAG AAGGCGCTCG CCAGAAACTA CAGCTGGAAA AGGTGACAGC GGAAGCAAAA 
ATCAAGAAGA TGGAGGAGGA GATCCTGCTG CTTGAGGACC AGAATTCCAA ATTTATCAAA 
GAAAAGAAAC TCATGGAAGA TCGCATTGCA GAGTGTTCCT CTCAGCTGGC TGAGGAGGAA 
GAAAAGGCCA AAAACTTGGC CAAAATCAGG AATAAGCAGG AAGTGATGAT CTCAGATTTA 
GAAGAACGCT TGAAGAAGGA AGAGAAGACG CGTCAGGAGC TGGAGAAGGC CAAACGAAAA 
CTGGACGGTG AGACCACTGA CCTGCAGGAC CAGATCGCAG AGCTCCAGGC GCAGATCGAC 
GAGCTCAAGG TCCAGCTGGC CAAGAAGGAG GAGGAGCTGC AAGGCGCGCT GGCCAGGGGT 
GACGATGAGA CGCTCCATAA GAACAATGCC CTCAAGGTGG TGCGAGAGCT GCAAGCCCAA 
ATCGCCGAGC TTCAGGAGGA CTTTGAATCA GAGAAGGCTT CACGGAACAA GGCGGAGAAG 
CAGAAGAGGG ACCTGAGTGA GGAGCTGGAG GCCCTGAAAA CAGAGCTGGA GGACACGCTG 
GACACCACCG CGGCTCAGCA GGAACTTCGC ACGAAGCGTG AACAAGAAGT GGCAGAGCTG 
AAGAAGGCTC TGGAGGAGGA AACGAGGAGC CACGAGGCTC AAATCCAGGA CATGAGACAG 
CGACACGCCA CAGCCCTGGA GGAGCTGTCG GAGCAGCTGG AACAGGCAAA GAGGTTCAAA 
GCGAATCTGG AGAAGAACAA GCAGGGCCTG GAGACTGACA ACAAGGAGCT GGCGTGTGAG 
GTGAAGGTGC TACAGCAGGT CAAGGCTGAG TCTGAGCACA AGAGGAAGAA GCTCGACGCC 
CAGGTGCAGG AGCTGCACGC CAAGGTCTCA GAAGGAGACA GGCTCAGGGT GGAGCTGGCC 
GAGAAGGCAA ACAAATTACA GAATGAACTG GACAATGTCT CAAGTCTTCT GGAAGAAGCA 
GAGAAGAAGG GCATTAAGTT TGCTAAGGAC GCAGCTAGTC TTGAGTCTCA ACTACAGGAC 
ACTCAGGAGC TCCTTCAGGA GGAGACCCGC CAGAAGCTGA ACCTGAGCAG CCGCATCCGG 
CAGCTGGAGG AGGAGAAGAA CAGTCTCCAG GAGCAGCAGG AGGAGGAGGA GGAGGCCAGG 
AAAAACCTGG AAAAGCAGGT GTTGGTGCTG CAGTCCCAGC TCGCCGATAC CAAGAAGAAG 
GTAGATGATG ACTTAGGAAC CATCGAGAGC TTAGAAGAAG CCAGAAAGAA GCTTCTGAAA 
GATGTGGAGG CCCTGGGCCA GCGCCTGGAG GAGAAGGCTC TGGCCTATGA CAAGCTGGAG 
AAGACCAAGA ACCGCCTGCA GCAGGAGCTC GACGACCTGA CAGTGGACCT AGATCACCAG 
CGCCAGATCG CCTCCAACTT GGAGAAGAAG CAGAAGAAGT TTGACCAGCT GCTGGCAGAG 
GAGAAGAACA TCTCCGCCCG CTATGCAGAA GAGCGAGACC GCGCAGAGGC AGAGGCCAGG 
GAGAAGGAAA CCAAAGCCCT GTCCCTGGCC CGAGCCCTCG AGGAGGCCCT AGAAACCAAG 
GAAGAGTTTG AGAGACAGAA CAAGCAGCTG CGGGCAGACA TGGAGGACCT GATAAGCTCC 
AAGGATGACG TGGGGAAGAA CGTGCACGAG CTTGAAAAAT CGAAGCGGGC CCTGGAGCAA 
CAGGTGGAGG AGATGAGGAC ACAGCTGGAA GAGCTGGAGG ATGAGCTTCA GGCCACAGAA 
GACGCCAAGC TTCGCCTGGA AGTCAACATG CAGGCCATGA AGGCCCAGTT TGAGAGGGAC 
TTACAAACCA GGGACGAGCA GAATGAAGAG AAGAAGCGGC TCCTGGTTAA GCAGGTGCGG 
GAGCTGGAGG CAGAGCTGGA AGATGAGCGG AAGCAGCGAG CGCTGGCGGT CGCTTCTAAA 
AAGAAACTGG AAATAGACCT GAAGGACCTT GAAGCCCAGA TTGAGGCTGC GAACAAAGCG 
AGGGACGAAG TGATCAAGCA GCTTCGCAAG CTTCAGGCTC AGATGAAGGA TTACCAACGT 
GAATTAGAAG AAGCCCGTGC ATCCCGGGAT GAGATTTTTG CTCAATCCAA AGAGAGTGAA 
AAGAAACTGA AGAGTCTGGA AGCAGAAATC CTTCAATTGC AGGAGGAACT GGCCTCATCT 
GAGCGAGCGC GCCGCCACGC CGAGCAGGAG AGAGATGAGC TGGCTGACGA GATCGCCAAC 
AGCGCCTCTG GCAAGTCTGC GCTGCTGGAT GAGAAGCGGC GTCTGGAGGC ACGGATCGCG 
CAGCTGGAGG AGGAGCTGGA AGAGGAGCAG AGCAACATGG AGCTGCTCAA CGACCGCTTC 
CGCAAGACCA CGCTGCAGGT GGACACGCTG AACGCCGAGC TCGCGGCTGA GCGCAGCGCC 
GCCCAGAAGA GCGACAACGC ACGCCAGCAG CTAGAGCGGC AGAACAAGGA GCTGAAGGCC 
AAGCTGCAGG AACTGGAGGG CGCCGTCAAG TCCAAGTTCA AGGCCACCAT CTCTGCCCTG 
GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG CTGGAGCAGG AAGCCAAGGA ACGAGCAGCC 
GCCAACAAAC TAGTCCGTCG CACGGAGAAA AAGCTGAAAG AGATCTTCAT GCAGGTTGAA 
GATGAGCGTC GACATGCGGA TCAGTATAAA GAACAGATGG AGAAGGCCAA CGCCAGGATG 
AAACAGCTCA AGCGGCAGCT GGAGGAGGCA GAGGAGGAAG CCACACGTGC CAACGCATCT 
CGGCGTAAGC TCCAGCGGGA ACTGGACGAT GCCACGGAGG CCAACGAGGG CCTGAGCCGT 
GAGGTCAGCA CGCTGAAGAA CCGGCTGAGA CGAGGTGGCC CCATAAGCTT CTCGTCCAGC 
CGATCGGGCC GGCGCCAGCT ACACATCGAG GGAGCCTCCC TGGAGCTGTC AGACGATGAC 
ACGGAGAGTA AGACCAGTGA CGTCAACGAG ACACAGCCGC CGCAGTCGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620096.1
CDS	35..6148
Translation	MRRAPRRPGPGAGANERPSRGNCPWILLFTMAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_005620039.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620039.2

ORF Insert Sequence:

ATGAGACGGG CGCCCCGGCG GCCTGGGCCG GGAGCGGGAG CCAATGAGCG TCCTTCCAGA 
GGCAATTGCC CTTGGATCCT CCTGTTTACA ATGGCCCAGA GAACTGGACT TGAAGACCCA 
GAGAGGTACC TCTTTGTGGA CAGGGCTGTC ATCTATAACC CTGCCACCCA GGCCGACTGG 
ACGGCTAAAA AGCTAGTGTG GATTCCATCA GAGCGCCATG GTTTCGAGGC AGCAAGTATC 
AAAGAGGAGC GGGGAGATGA GGTTATGGTG GAGTTGGCAG AGAACGGCAA GAAAGCCATG 
GTCAACAAAG ACGATATTCA GAAGATGAAC CCACCTAAGT TTTCCAAGGT GGAGGATATG 
GCGGAATTGA CGTGCTTGAA CGAAGCTTCT GTTCTACATA ACCTGAAGGA CCGCTACTAT 
TCGGGATTGA TCTATACTTA CTCTGGACTC TTCTGTGTAG TCATAAATCC TTACAAGAAT 
CTTCCAATTT ACTCTGAGAA TATTATTGAA ATGTACAGAG GGAAGAAACG CCACGAGATG 
CCTCCACACA TCTACGCTAT ATCTGAATCT GCTTACAGAT GCATGCTTCA AGATCGTGAG 
GACCAGTCCA TTCTTTGCAC GGGCGAATCA GGTGCTGGGA AGACAGAAAA TACCAAGAAA 
GTCATTCAGT ACCTTGCTCA TGTTGCTTCT TCGCATAAAG GACGGAAGGA CCATAATATT 
CCTCAGGAAT CGCCTAAACC AGTGAAACAT CAGGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC CTTTGGAAAT GCGAAGACTG TGAAAAATGA TAATTCATCT 
CGCTTTGGCA AGTTTATTCG AATCAACTTT GATGTAACTG GCTATATTGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAGTT GTTATCTGGA GCAGGAGAAC ACCTAAAGTC GGATTTGCTC 
CTCGAAGGAT TTAATAACTA CAGATTCCTC TCCAACGGCT ATATTCCCAT CCCGGGACAG 
CAAGACAAGG ACAACTTTCA GGAAACCATG GAAGCAATGC ACATCATGGG CTTCTCTCAC 
GAGGAGATTC TGTCAATGCT TAAAGTAGTG TCATCAGTGC TACAGTTCGG AAATATTTCT 
TTCAAAAAGG AGCGAAATAC TGATCAAGCA TCCATGCCAG AGAACACAGT TGCACAGAAA 
CTCTGCCATC TTCTTGGGAT GAACGTGATG GAGTTCACGC GGGCCATCCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA TGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCG 
GTAGAAGCAT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GTTGGCTCGT TCACCGTATC 
AACAAAGCTC TGGACAGGAC CAAGCGTCAG GGCGCATCTT TTATTGGAAT CCTGGATATC 
GCTGGATTCG AAATATTTGA GCTCAACTCG TTCGAGCAAC TGTGCATCAA CTACACCAAT 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAG CTTCATTGAC TTTGGCCTCG ACCTGCAGCC CTGCATCGAC 
CTCATCGAGA GACCCGCTAA CCCCCCTGGT GTGCTGGCCC TTTTGGATGA AGAGTGCTGG 
TTCCCGAAGG CCACGGACAA AACCTTTGTT GAAAAACTAG TTCAAGAGCA AGGCTCTCAC 
TCCAAGTTCC AGAAACCCCG GCAACTGAAA GACAGAGCTG ACTTCTGCAT CATCCATTAT 
GCGGGCAAGG TGGACTACAA GGCTGATGAG TGGCTGATGA AGAACATGGA CCCCCTGAAC 
GACAATGTGG CCACCCTCCT GCACCAGTCA TCTGACAGAT TCGTGGCAGA GCTTTGGAAG 
GATGAGATTC AGAATATTCA GAGAGCTTCT TTCTATGACA GTGTTTCTGG TCTTCATGAG 
CCACCAGTGG ACCGTATCGT GGGTCTGGAT CAGGTCACGG GCATGACGGA GACAGCCTTT 
GGCTCTGCGT ATAAAACCAA GAAGGGCATG TTTCGGACTG TCGGGCAGCT CTACAAAGAA 
TCCCTCACCA AGCTGATGGC AACTCTCCGG AACACCAACC CTAACTTCGT CCGCTGCATC 
ATTCCAAATC ACGAGAAGCG AGCTGGAAAA CTGGATCCGC ACCTAGTGCT TGATCAGCTT 
CGCTGTAACG GCGTTCTGGA AGGAATCAGG ATCTGTCGCC AGGGGTTCCC CAACCGGATA 
GTTTTCCAGG AATTCAGACA GAGATACGAG ATCCTAACTC CAAATGCTAT CCCTAAGGGT 
TTCATGGACG GCAAACAGGC CTGCGAACGA ATGATCCGGG CTTTAGAACT GGACCCAAAC 
TTGTACAGAA TCGGACAGAG CAAGATATTT TTCCGAGCTG GCGTGCTGGC ACACTTAGAG 
GAAGAGAGGG ATCTAAAGAT CACCGACATC ATTATCTTCT TCCAGGCTGT CTGCAGAGGC 
TACCTGGCCA GAAAGGCCTT CGCCAAGAAG CAGCAGCAGC TGAGCGCCCT GAAGGTCCTG 
CAGCGGAACT GCGCCGCATA CCTGAAGCTC CGGCACTGGC AGTGGTGGCG TGTCTTCACG 
AAGGTGAAGC CGCTCCTGCA AGTGACCCGC CAGGAGGAAG AGCTTCAGGC CAAAGACGAG 
GAGCTGCTGA AGGTGAAGGA GAGGCAGACG AAGGTGGAGG GCGAGCTGGA GGAAATGGAG 
AGGAAGCACC AGCAGCTCCT GGAAGAGAAG AACATCCTAA CAGAGCAGCT CCAAGCAGAG 
ACAGAGCTCT TTGCTGAGGC AGAAGAGATG AGAGCCAGGC TCGCCGCCAA GAAGCAGGAG 
CTGGAGGAGA TTCTCCATGA CTTGGAATCC AGGGTCGAAG AGGAAGAAGA ACGAAACCAA 
ATCCTCCAAA ATGAAAAGAA GAAAATGCAA GCTCACATTC AGGACCTAGA GGAGCAACTG 
GATGAGGAAG AAGGCGCTCG CCAGAAACTA CAGCTGGAAA AGGTGACAGC GGAAGCAAAA 
ATCAAGAAGA TGGAGGAGGA GATCCTGCTG CTTGAGGACC AGAATTCCAA ATTTATCAAA 
GAAAAGAAAC TCATGGAAGA TCGCATTGCA GAGTGTTCCT CTCAGCTGGC TGAGGAGGAA 
GAAAAGGCCA AAAACTTGGC CAAAATCAGG AATAAGCAGG AAGTGATGAT CTCAGATTTA 
GAAGAACGCT TGAAGAAGGA AGAGAAGACG CGTCAGGAGC TGGAGAAGGC CAAACGAAAA 
CTGGACGGTG AGACCACTGA CCTGCAGGAC CAGATCGCAG AGCTCCAGGC GCAGATCGAC 
GAGCTCAAGG TCCAGCTGGC CAAGAAGGAG GAGGAGCTGC AAGGCGCGCT GGCCAGGGGT 
GACGATGAGA CGCTCCATAA GAACAATGCC CTCAAGGTGG TGCGAGAGCT GCAAGCCCAA 
ATCGCCGAGC TTCAGGAGGA CTTTGAATCA GAGAAGGCTT CACGGAACAA GGCGGAGAAG 
CAGAAGAGGG ACCTGAGTGA GGAGCTGGAG GCCCTGAAAA CAGAGCTGGA GGACACGCTG 
GACACCACCG CGGCTCAGCA GGAACTTCGC ACGAAGCGTG AACAAGAAGT GGCAGAGCTG 
AAGAAGGCTC TGGAGGAGGA AACGAGGAGC CACGAGGCTC AAATCCAGGA CATGAGACAG 
CGACACGCCA CAGCCCTGGA GGAGCTGTCG GAGCAGCTGG AACAGGCAAA GAGGTTCAAA 
GCGAATCTGG AGAAGAACAA GCAGGGCCTG GAGACTGACA ACAAGGAGCT GGCGTGTGAG 
GTGAAGGTGC TACAGCAGGT CAAGGCTGAG TCTGAGCACA AGAGGAAGAA GCTCGACGCC 
CAGGTGCAGG AGCTGCACGC CAAGGTCTCA GAAGGAGACA GGCTCAGGGT GGAGCTGGCC 
GAGAAGGCAA ACAAATTACA GAATGAACTG GACAATGTCT CAAGTCTTCT GGAAGAAGCA 
GAGAAGAAGG GCATTAAGTT TGCTAAGGAC GCAGCTAGTC TTGAGTCTCA ACTACAGGAC 
ACTCAGGAGC TCCTTCAGGA GGAGACCCGC CAGAAGCTGA ACCTGAGCAG CCGCATCCGG 
CAGCTGGAGG AGGAGAAGAA CAGTCTCCAG GAGCAGCAGG AGGAGGAGGA GGAGGCCAGG 
AAAAACCTGG AAAAGCAGGT GTTGGTGCTG CAGTCCCAGC TCGCCGATAC CAAGAAGAAG 
GTAGATGATG ACTTAGGAAC CATCGAGAGC TTAGAAGAAG CCAGAAAGAA GCTTCTGAAA 
GATGTGGAGG CCCTGGGCCA GCGCCTGGAG GAGAAGGCTC TGGCCTATGA CAAGCTGGAG 
AAGACCAAGA ACCGCCTGCA GCAGGAGCTC GACGACCTGA CAGTGGACCT AGATCACCAG 
CGCCAGATCG CCTCCAACTT GGAGAAGAAG CAGAAGAAGT TTGACCAGCT GCTGGCAGAG 
GAGAAGAACA TCTCCGCCCG CTATGCAGAA GAGCGAGACC GCGCAGAGGC AGAGGCCAGG 
GAGAAGGAAA CCAAAGCCCT GTCCCTGGCC CGAGCCCTCG AGGAGGCCCT AGAAACCAAG 
GAAGAGTTTG AGAGACAGAA CAAGCAGCTG CGGGCAGACA TGGAGGACCT GATAAGCTCC 
AAGGATGACG TGGGGAAGAA CGTGCACGAG CTTGAAAAAT CGAAGCGGGC CCTGGAGCAA 
CAGGTGGAGG AGATGAGGAC ACAGCTGGAA GAGCTGGAGG ATGAGCTTCA GGCCACAGAA 
GACGCCAAGC TTCGCCTGGA AGTCAACATG CAGGCCATGA AGGCCCAGTT TGAGAGGGAC 
TTACAAACCA GGGACGAGCA GAATGAAGAG AAGAAGCGGC TCCTGGTTAA GCAGGTGCGG 
GAGCTGGAGG CAGAGCTGGA AGATGAGCGG AAGCAGCGAG CGCTGGCGGT CGCTTCTAAA 
AAGAAACTGG AAATAGACCT GAAGGACCTT GAAGCCCAGA TTGAGGCTGC GAACAAAGCG 
AGGGACGAAG TGATCAAGCA GCTTCGCAAG CTTCAGGCTC AGATGAAGGA TTACCAACGT 
GAATTAGAAG AAGCCCGTGC ATCCCGGGAT GAGATTTTTG CTCAATCCAA AGAGAGTGAA 
AAGAAACTGA AGAGTCTGGA AGCAGAAATC CTTCAATTGC AGGAGGAACT GGCCTCATCT 
GAGCGAGCGC GCCGCCACGC CGAGCAGGAG AGAGATGAGC TGGCTGACGA GATCGCCAAC 
AGCGCCTCTG GCAAGTCTGC GCTGCTGGAT GAGAAGCGGC GTCTGGAGGC ACGGATCGCG 
CAGCTGGAGG AGGAGCTGGA AGAGGAGCAG AGCAACATGG AGCTGCTCAA CGACCGCTTC 
CGCAAGACCA CGCTGCAGGT GGACACGCTG AACGCCGAGC TCGCGGCTGA GCGCAGCGCC 
GCCCAGAAGA GCGACAACGC ACGCCAGCAG CTAGAGCGGC AGAACAAGGA GCTGAAGGCC 
AAGCTGCAGG AACTGGAGGG CGCCGTCAAG TCCAAGTTCA AGGCCACCAT CTCTGCCCTG 
GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG CTGGAGCAGG AAGCCAAGGA ACGAGCAGCC 
GCCAACAAAC TAGTCCGTCG CACGGAGAAA AAGCTGAAAG AGATCTTCAT GCAGGTTGAA 
GATGAGCGTC GACATGCGGA TCAGTATAAA GAACAGATGG AGAAGGCCAA CGCCAGGATG 
AAACAGCTCA AGCGGCAGCT GGAGGAGGCA GAGGAGGAAG CCACACGTGC CAACGCATCT 
CGGCGTAAGC TCCAGCGGGA ACTGGACGAT GCCACGGAGG CCAACGAGGG CCTGAGCCGT 
GAGGTCAGCA CGCTGAAGAA CCGGCTGAGA CGAGGTGGCC CCATAAGCTT CTCGTCCAGC 
CGATCGGGCC GGCGCCAGCT ACACATCGAG GGAGCCTCCC TGGAGCTGTC AGACGATGAC 
ACGGAGAGTA AGACCAGTGA CGTCAACGAG ACACAGCCGC CGCAGTCGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_005620039.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620096.2 Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X7, mRNA.

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CloneID	OCe36456
Clone ID Related Accession (Same CDS sequence)	XM_005620039.3 , XM_014113594.1 , XM_014113594.2
Accession Version	XM_005620039.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	myosin-10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620039.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTCAGGAAT CGCCTAAACC AGTGAAACAT 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC CTTTGGAAAT 
GCGAAGACTG TGAAAAATGA TAATTCATCT CGCTTTGGCA AGTTTATTCG AATCAACTTT 
GATGTAACTG GCTATATTGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAGTT GTTATCTGGA 
GCAGGAGAAC ACCTAAAGTC GGATTTGCTC CTCGAAGGAT TTAATAACTA CAGATTCCTC 
TCCAACGGCT ATATTCCCAT CCCGGGACAG CAAGACAAGG ACAACTTTCA GGAAACCATG 
GAAGCAATGC ACATCATGGG CTTCTCTCAC GAGGAGATTC TGTCAATGCT TAAAGTAGTG 
TCATCAGTGC TACAGTTCGG AAATATTTCT TTCAAAAAGG AGCGAAATAC TGATCAAGCA 
TCCATGCCAG AGAACACAGT TGCACAGAAA CTCTGCCATC TTCTTGGGAT GAACGTGATG 
GAGTTCACGC GGGCCATCCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA TGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCG GTAGAAGCAT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GTTGGCTCGT TCACCGTATC AACAAAGCTC TGGACAGGAC CAAGCGTCAG 
GGCGCATCTT TTATTGGAAT CCTGGATATC GCTGGATTCG AAATATTTGA GCTCAACTCG 
TTCGAGCAAC TGTGCATCAA CTACACCAAT GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAG CTTCATTGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATCGAC CTCATCGAGA GACCCGCTAA CCCCCCTGGT 
GTGCTGGCCC TTTTGGATGA AGAGTGCTGG TTCCCGAAGG CCACGGACAA AACCTTTGTT 
GAAAAACTAG TTCAAGAGCA AGGCTCTCAC TCCAAGTTCC AGAAACCCCG GCAACTGAAA 
GACAGAGCTG ACTTCTGCAT CATCCATTAT GCGGGCAAGG TGGACTACAA GGCTGATGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAC GACAATGTGG CCACCCTCCT GCACCAGTCA 
TCTGACAGAT TCGTGGCAGA GCTTTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGTATCGT GGGTCTGGAT 
CAGGTCACGG GCATGACGGA GACAGCCTTT GGCTCTGCGT ATAAAACCAA GAAGGGCATG 
TTTCGGACTG TCGGGCAGCT CTACAAAGAA TCCCTCACCA AGCTGATGGC AACTCTCCGG 
AACACCAACC CTAACTTCGT CCGCTGCATC ATTCCAAATC ACGAGAAGCG AGCTGGAAAA 
CTGGATCCGC ACCTAGTGCT TGATCAGCTT CGCTGTAACG GCGTTCTGGA AGGAATCAGG 
ATCTGTCGCC AGGGGTTCCC CAACCGGATA GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTAACTC CAAATGCTAT CCCTAAGGGT TTCATGGACG GCAAACAGGC CTGCGAACGA 
ATGATCCGGG CTTTAGAACT GGACCCAAAC TTGTACAGAA TCGGACAGAG CAAGATATTT 
TTCCGAGCTG GCGTGCTGGC ACACTTAGAG GAAGAGAGGG ATCTAAAGAT CACCGACATC 
ATTATCTTCT TCCAGGCTGT CTGCAGAGGC TACCTGGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGCGCCCT GAAGGTCCTG CAGCGGAACT GCGCCGCATA CCTGAAGCTC 
CGGCACTGGC AGTGGTGGCG TGTCTTCACG AAGGTGAAGC CGCTCCTGCA AGTGACCCGC 
CAGGAGGAAG AGCTTCAGGC CAAAGACGAG GAGCTGCTGA AGGTGAAGGA GAGGCAGACG 
AAGGTGGAGG GCGAGCTGGA GGAAATGGAG AGGAAGCACC AGCAGCTCCT GGAAGAGAAG 
AACATCCTAA CAGAGCAGCT CCAAGCAGAG ACAGAGCTCT TTGCTGAGGC AGAAGAGATG 
AGAGCCAGGC TCGCCGCCAA GAAGCAGGAG CTGGAGGAGA TTCTCCATGA CTTGGAATCC 
AGGGTCGAAG AGGAAGAAGA ACGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCTCACATTC AGGACCTAGA GGAGCAACTG GATGAGGAAG AAGGCGCTCG CCAGAAACTA 
CAGCTGGAAA AGGTGACAGC GGAAGCAAAA ATCAAGAAGA TGGAGGAGGA GATCCTGCTG 
CTTGAGGACC AGAATTCCAA ATTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAGTGTTCCT CTCAGCTGGC TGAGGAGGAA GAAAAGGCCA AAAACTTGGC CAAAATCAGG 
AATAAGCAGG AAGTGATGAT CTCAGATTTA GAAGAACGCT TGAAGAAGGA AGAGAAGACG 
CGTCAGGAGC TGGAGAAGGC CAAACGAAAA CTGGACGGTG AGACCACTGA CCTGCAGGAC 
CAGATCGCAG AGCTCCAGGC GCAGATCGAC GAGCTCAAGG TCCAGCTGGC CAAGAAGGAG 
GAGGAGCTGC AAGGCGCGCT GGCCAGGGGT GACGATGAGA CGCTCCATAA GAACAATGCC 
CTCAAGGTGG TGCGAGAGCT GCAAGCCCAA ATCGCCGAGC TTCAGGAGGA CTTTGAATCA 
GAGAAGGCTT CACGGAACAA GGCGGAGAAG CAGAAGAGGG ACCTGAGTGA GGAGCTGGAG 
GCCCTGAAAA CAGAGCTGGA GGACACGCTG GACACCACCG CGGCTCAGCA GGAACTTCGC 
ACGAAGCGTG AACAAGAAGT GGCAGAGCTG AAGAAGGCTC TGGAGGAGGA AACGAGGAGC 
CACGAGGCTC AAATCCAGGA CATGAGACAG CGACACGCCA CAGCCCTGGA GGAGCTGTCG 
GAGCAGCTGG AACAGGCAAA GAGGTTCAAA GCGAATCTGG AGAAGAACAA GCAGGGCCTG 
GAGACTGACA ACAAGGAGCT GGCGTGTGAG GTGAAGGTGC TACAGCAGGT CAAGGCTGAG 
TCTGAGCACA AGAGGAAGAA GCTCGACGCC CAGGTGCAGG AGCTGCACGC CAAGGTCTCA 
GAAGGAGACA GGCTCAGGGT GGAGCTGGCC GAGAAGGCAA ACAAATTACA GAATGAACTG 
GACAATGTCT CAAGTCTTCT GGAAGAAGCA GAGAAGAAGG GCATTAAGTT TGCTAAGGAC 
GCAGCTAGTC TTGAGTCTCA ACTACAGGAC ACTCAGGAGC TCCTTCAGGA GGAGACCCGC 
CAGAAGCTGA ACCTGAGCAG CCGCATCCGG CAGCTGGAGG AGGAGAAGAA CAGTCTCCAG 
GAGCAGCAGG AGGAGGAGGA GGAGGCCAGG AAAAACCTGG AAAAGCAGGT GTTGGTGCTG 
CAGTCCCAGC TCGCCGATAC CAAGAAGAAG GTAGATGATG ACTTAGGAAC CATCGAGAGC 
TTAGAAGAAG CCAGAAAGAA GCTTCTGAAA GATGTGGAGG CCCTGGGCCA GCGCCTGGAG 
GAGAAGGCTC TGGCCTATGA CAAGCTGGAG AAGACCAAGA ACCGCCTGCA GCAGGAGCTC 
GACGACCTGA CAGTGGACCT AGATCACCAG CGCCAGATCG CCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGACCAGCT GCTGGCAGAG GAGAAGAACA TCTCCGCCCG CTATGCAGAA 
GAGCGAGACC GCGCAGAGGC AGAGGCCAGG GAGAAGGAAA CCAAAGCCCT GTCCCTGGCC 
CGAGCCCTCG AGGAGGCCCT AGAAACCAAG GAAGAGTTTG AGAGACAGAA CAAGCAGCTG 
CGGGCAGACA TGGAGGACCT GATAAGCTCC AAGGATGACG TGGGGAAGAA CGTGCACGAG 
CTTGAAAAAT CGAAGCGGGC CCTGGAGCAA CAGGTGGAGG AGATGAGGAC ACAGCTGGAA 
GAGCTGGAGG ATGAGCTTCA GGCCACAGAA GACGCCAAGC TTCGCCTGGA AGTCAACATG 
CAGGCCATGA AGGCCCAGTT TGAGAGGGAC TTACAAACCA GGGACGAGCA GAATGAAGAG 
AAGAAGCGGC TCCTGGTTAA GCAGGTGCGG GAGCTGGAGG CAGAGCTGGA AGATGAGCGG 
AAGCAGCGAG CGCTGGCGGT CGCTTCTAAA AAGAAACTGG AAATAGACCT GAAGGACCTT 
GAAGCCCAGA TTGAGGCTGC GAACAAAGCG AGGGACGAAG TGATCAAGCA GCTTCGCAAG 
CTTCAGGCTC AGATGAAGGA TTACCAACGT GAATTAGAAG AAGCCCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAA AAGAAACTGA AGAGTCTGGA AGCAGAAATC 
CTTCAATTGC AGGAGGAACT GGCCTCATCT GAGCGAGCGC GCCGCCACGC CGAGCAGGAG 
AGAGATGAGC TGGCTGACGA GATCGCCAAC AGCGCCTCTG GCAAGTCTGC GCTGCTGGAT 
GAGAAGCGGC GTCTGGAGGC ACGGATCGCG CAGCTGGAGG AGGAGCTGGA AGAGGAGCAG 
AGCAACATGG AGCTGCTCAA CGACCGCTTC CGCAAGACCA CGCTGCAGGT GGACACGCTG 
AACGCCGAGC TCGCGGCTGA GCGCAGCGCC GCCCAGAAGA GCGACAACGC ACGCCAGCAG 
CTAGAGCGGC AGAACAAGGA GCTGAAGGCC AAGCTGCAGG AACTGGAGGG CGCCGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCCCTG GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTGGAGCAGG AAGCCAAGGA ACGAGCAGCC GCCAACAAAC TAGTCCGTCG CACGGAGAAA 
AAGCTGAAAG AGATCTTCAT GCAGGTTGAA GATGAGCGTC GACATGCGGA TCAGTATAAA 
GAACAGATGG AGAAGGCCAA CGCCAGGATG AAACAGCTCA AGCGGCAGCT GGAGGAGGCA 
GAGGAGGAAG CCACACGTGC CAACGCATCT CGGCGTAAGC TCCAGCGGGA ACTGGACGAT 
GCCACGGAGG CCAACGAGGG CCTGAGCCGT GAGGTCAGCA CGCTGAAGAA CCGGCTGAGA 
CGAGGTGGCC CCATAAGCTT CTCGTCCAGC CGATCGGGCC GGCGCCAGCT ACACATCGAG 
GGAGCCTCCC TGGAGCTGTC AGACGATGAC ACGGAGAGTA AGACCAGTGA CGTCAACGAG 
ACACAGCCGC CGCAGTCGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620096.2
CDS	51..6074
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_005620039.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620039.3

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTCAGGAAT CGCCTAAACC AGTGAAACAT 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC CTTTGGAAAT 
GCGAAGACTG TGAAAAATGA TAATTCATCT CGCTTTGGCA AGTTTATTCG AATCAACTTT 
GATGTAACTG GCTATATTGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAGTT GTTATCTGGA 
GCAGGAGAAC ACCTAAAGTC GGATTTGCTC CTCGAAGGAT TTAATAACTA CAGATTCCTC 
TCCAACGGCT ATATTCCCAT CCCGGGACAG CAAGACAAGG ACAACTTTCA GGAAACCATG 
GAAGCAATGC ACATCATGGG CTTCTCTCAC GAGGAGATTC TGTCAATGCT TAAAGTAGTG 
TCATCAGTGC TACAGTTCGG AAATATTTCT TTCAAAAAGG AGCGAAATAC TGATCAAGCA 
TCCATGCCAG AGAACACAGT TGCACAGAAA CTCTGCCATC TTCTTGGGAT GAACGTGATG 
GAGTTCACGC GGGCCATCCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA TGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCG GTAGAAGCAT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GTTGGCTCGT TCACCGTATC AACAAAGCTC TGGACAGGAC CAAGCGTCAG 
GGCGCATCTT TTATTGGAAT CCTGGATATC GCTGGATTCG AAATATTTGA GCTCAACTCG 
TTCGAGCAAC TGTGCATCAA CTACACCAAT GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAG CTTCATTGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATCGAC CTCATCGAGA GACCCGCTAA CCCCCCTGGT 
GTGCTGGCCC TTTTGGATGA AGAGTGCTGG TTCCCGAAGG CCACGGACAA AACCTTTGTT 
GAAAAACTAG TTCAAGAGCA AGGCTCTCAC TCCAAGTTCC AGAAACCCCG GCAACTGAAA 
GACAGAGCTG ACTTCTGCAT CATCCATTAT GCGGGCAAGG TGGACTACAA GGCTGATGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAC GACAATGTGG CCACCCTCCT GCACCAGTCA 
TCTGACAGAT TCGTGGCAGA GCTTTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGTATCGT GGGTCTGGAT 
CAGGTCACGG GCATGACGGA GACAGCCTTT GGCTCTGCGT ATAAAACCAA GAAGGGCATG 
TTTCGGACTG TCGGGCAGCT CTACAAAGAA TCCCTCACCA AGCTGATGGC AACTCTCCGG 
AACACCAACC CTAACTTCGT CCGCTGCATC ATTCCAAATC ACGAGAAGCG AGCTGGAAAA 
CTGGATCCGC ACCTAGTGCT TGATCAGCTT CGCTGTAACG GCGTTCTGGA AGGAATCAGG 
ATCTGTCGCC AGGGGTTCCC CAACCGGATA GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTAACTC CAAATGCTAT CCCTAAGGGT TTCATGGACG GCAAACAGGC CTGCGAACGA 
ATGATCCGGG CTTTAGAACT GGACCCAAAC TTGTACAGAA TCGGACAGAG CAAGATATTT 
TTCCGAGCTG GCGTGCTGGC ACACTTAGAG GAAGAGAGGG ATCTAAAGAT CACCGACATC 
ATTATCTTCT TCCAGGCTGT CTGCAGAGGC TACCTGGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGCGCCCT GAAGGTCCTG CAGCGGAACT GCGCCGCATA CCTGAAGCTC 
CGGCACTGGC AGTGGTGGCG TGTCTTCACG AAGGTGAAGC CGCTCCTGCA AGTGACCCGC 
CAGGAGGAAG AGCTTCAGGC CAAAGACGAG GAGCTGCTGA AGGTGAAGGA GAGGCAGACG 
AAGGTGGAGG GCGAGCTGGA GGAAATGGAG AGGAAGCACC AGCAGCTCCT GGAAGAGAAG 
AACATCCTAA CAGAGCAGCT CCAAGCAGAG ACAGAGCTCT TTGCTGAGGC AGAAGAGATG 
AGAGCCAGGC TCGCCGCCAA GAAGCAGGAG CTGGAGGAGA TTCTCCATGA CTTGGAATCC 
AGGGTCGAAG AGGAAGAAGA ACGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCTCACATTC AGGACCTAGA GGAGCAACTG GATGAGGAAG AAGGCGCTCG CCAGAAACTA 
CAGCTGGAAA AGGTGACAGC GGAAGCAAAA ATCAAGAAGA TGGAGGAGGA GATCCTGCTG 
CTTGAGGACC AGAATTCCAA ATTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAGTGTTCCT CTCAGCTGGC TGAGGAGGAA GAAAAGGCCA AAAACTTGGC CAAAATCAGG 
AATAAGCAGG AAGTGATGAT CTCAGATTTA GAAGAACGCT TGAAGAAGGA AGAGAAGACG 
CGTCAGGAGC TGGAGAAGGC CAAACGAAAA CTGGACGGTG AGACCACTGA CCTGCAGGAC 
CAGATCGCAG AGCTCCAGGC GCAGATCGAC GAGCTCAAGG TCCAGCTGGC CAAGAAGGAG 
GAGGAGCTGC AAGGCGCGCT GGCCAGGGGT GACGATGAGA CGCTCCATAA GAACAATGCC 
CTCAAGGTGG TGCGAGAGCT GCAAGCCCAA ATCGCCGAGC TTCAGGAGGA CTTTGAATCA 
GAGAAGGCTT CACGGAACAA GGCGGAGAAG CAGAAGAGGG ACCTGAGTGA GGAGCTGGAG 
GCCCTGAAAA CAGAGCTGGA GGACACGCTG GACACCACCG CGGCTCAGCA GGAACTTCGC 
ACGAAGCGTG AACAAGAAGT GGCAGAGCTG AAGAAGGCTC TGGAGGAGGA AACGAGGAGC 
CACGAGGCTC AAATCCAGGA CATGAGACAG CGACACGCCA CAGCCCTGGA GGAGCTGTCG 
GAGCAGCTGG AACAGGCAAA GAGGTTCAAA GCGAATCTGG AGAAGAACAA GCAGGGCCTG 
GAGACTGACA ACAAGGAGCT GGCGTGTGAG GTGAAGGTGC TACAGCAGGT CAAGGCTGAG 
TCTGAGCACA AGAGGAAGAA GCTCGACGCC CAGGTGCAGG AGCTGCACGC CAAGGTCTCA 
GAAGGAGACA GGCTCAGGGT GGAGCTGGCC GAGAAGGCAA ACAAATTACA GAATGAACTG 
GACAATGTCT CAAGTCTTCT GGAAGAAGCA GAGAAGAAGG GCATTAAGTT TGCTAAGGAC 
GCAGCTAGTC TTGAGTCTCA ACTACAGGAC ACTCAGGAGC TCCTTCAGGA GGAGACCCGC 
CAGAAGCTGA ACCTGAGCAG CCGCATCCGG CAGCTGGAGG AGGAGAAGAA CAGTCTCCAG 
GAGCAGCAGG AGGAGGAGGA GGAGGCCAGG AAAAACCTGG AAAAGCAGGT GTTGGTGCTG 
CAGTCCCAGC TCGCCGATAC CAAGAAGAAG GTAGATGATG ACTTAGGAAC CATCGAGAGC 
TTAGAAGAAG CCAGAAAGAA GCTTCTGAAA GATGTGGAGG CCCTGGGCCA GCGCCTGGAG 
GAGAAGGCTC TGGCCTATGA CAAGCTGGAG AAGACCAAGA ACCGCCTGCA GCAGGAGCTC 
GACGACCTGA CAGTGGACCT AGATCACCAG CGCCAGATCG CCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGACCAGCT GCTGGCAGAG GAGAAGAACA TCTCCGCCCG CTATGCAGAA 
GAGCGAGACC GCGCAGAGGC AGAGGCCAGG GAGAAGGAAA CCAAAGCCCT GTCCCTGGCC 
CGAGCCCTCG AGGAGGCCCT AGAAACCAAG GAAGAGTTTG AGAGACAGAA CAAGCAGCTG 
CGGGCAGACA TGGAGGACCT GATAAGCTCC AAGGATGACG TGGGGAAGAA CGTGCACGAG 
CTTGAAAAAT CGAAGCGGGC CCTGGAGCAA CAGGTGGAGG AGATGAGGAC ACAGCTGGAA 
GAGCTGGAGG ATGAGCTTCA GGCCACAGAA GACGCCAAGC TTCGCCTGGA AGTCAACATG 
CAGGCCATGA AGGCCCAGTT TGAGAGGGAC TTACAAACCA GGGACGAGCA GAATGAAGAG 
AAGAAGCGGC TCCTGGTTAA GCAGGTGCGG GAGCTGGAGG CAGAGCTGGA AGATGAGCGG 
AAGCAGCGAG CGCTGGCGGT CGCTTCTAAA AAGAAACTGG AAATAGACCT GAAGGACCTT 
GAAGCCCAGA TTGAGGCTGC GAACAAAGCG AGGGACGAAG TGATCAAGCA GCTTCGCAAG 
CTTCAGGCTC AGATGAAGGA TTACCAACGT GAATTAGAAG AAGCCCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAA AAGAAACTGA AGAGTCTGGA AGCAGAAATC 
CTTCAATTGC AGGAGGAACT GGCCTCATCT GAGCGAGCGC GCCGCCACGC CGAGCAGGAG 
AGAGATGAGC TGGCTGACGA GATCGCCAAC AGCGCCTCTG GCAAGTCTGC GCTGCTGGAT 
GAGAAGCGGC GTCTGGAGGC ACGGATCGCG CAGCTGGAGG AGGAGCTGGA AGAGGAGCAG 
AGCAACATGG AGCTGCTCAA CGACCGCTTC CGCAAGACCA CGCTGCAGGT GGACACGCTG 
AACGCCGAGC TCGCGGCTGA GCGCAGCGCC GCCCAGAAGA GCGACAACGC ACGCCAGCAG 
CTAGAGCGGC AGAACAAGGA GCTGAAGGCC AAGCTGCAGG AACTGGAGGG CGCCGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCCCTG GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTGGAGCAGG AAGCCAAGGA ACGAGCAGCC GCCAACAAAC TAGTCCGTCG CACGGAGAAA 
AAGCTGAAAG AGATCTTCAT GCAGGTTGAA GATGAGCGTC GACATGCGGA TCAGTATAAA 
GAACAGATGG AGAAGGCCAA CGCCAGGATG AAACAGCTCA AGCGGCAGCT GGAGGAGGCA 
GAGGAGGAAG CCACACGTGC CAACGCATCT CGGCGTAAGC TCCAGCGGGA ACTGGACGAT 
GCCACGGAGG CCAACGAGGG CCTGAGCCGT GAGGTCAGCA CGCTGAAGAA CCGGCTGAGA 
CGAGGTGGCC CCATAAGCTT CTCGTCCAGC CGATCGGGCC GGCGCCAGCT ACACATCGAG 
GGAGCCTCCC TGGAGCTGTC AGACGATGAC ACGGAGAGTA AGACCAGTGA CGTCAACGAG 
ACACAGCCGC CGCAGTCGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_014113594.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969069.1 Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X1, mRNA.

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CloneID	OCe36456
Clone ID Related Accession (Same CDS sequence)	XM_005620039.3 , XM_014113594.1 , XM_014113594.2
Accession Version	XM_014113594.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	myosin-10 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTCAGGAAT CGCCTAAACC AGTGAAACAT 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC CTTTGGAAAT 
GCGAAGACTG TGAAAAATGA TAATTCATCT CGCTTTGGCA AGTTTATTCG AATCAACTTT 
GATGTAACTG GCTATATTGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAGTT GTTATCTGGA 
GCAGGAGAAC ACCTAAAGTC GGATTTGCTC CTCGAAGGAT TTAATAACTA CAGATTCCTC 
TCCAACGGCT ATATTCCCAT CCCGGGACAG CAAGACAAGG ACAACTTTCA GGAAACCATG 
GAAGCAATGC ACATCATGGG CTTCTCTCAC GAGGAGATTC TGTCAATGCT TAAAGTAGTG 
TCATCAGTGC TACAGTTCGG AAATATTTCT TTCAAAAAGG AGCGAAATAC TGATCAAGCA 
TCCATGCCAG AGAACACAGT TGCACAGAAA CTCTGCCATC TTCTTGGGAT GAACGTGATG 
GAGTTCACGC GGGCCATCCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA TGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCG GTAGAAGCAT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GTTGGCTCGT TCACCGTATC AACAAAGCTC TGGACAGGAC CAAGCGTCAG 
GGCGCATCTT TTATTGGAAT CCTGGATATC GCTGGATTCG AAATATTTGA GCTCAACTCG 
TTCGAGCAAC TGTGCATCAA CTACACCAAT GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAG CTTCATTGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATCGAC CTCATCGAGA GACCCGCTAA CCCCCCTGGT 
GTGCTGGCCC TTTTGGATGA AGAGTGCTGG TTCCCGAAGG CCACGGACAA AACCTTTGTT 
GAAAAACTAG TTCAAGAGCA AGGCTCTCAC TCCAAGTTCC AGAAACCCCG GCAACTGAAA 
GACAGAGCTG ACTTCTGCAT CATCCATTAT GCGGGCAAGG TGGACTACAA GGCTGATGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAC GACAATGTGG CCACCCTCCT GCACCAGTCA 
TCTGACAGAT TCGTGGCAGA GCTTTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGTATCGT GGGTCTGGAT 
CAGGTCACGG GCATGACGGA GACAGCCTTT GGCTCTGCGT ATAAAACCAA GAAGGGCATG 
TTTCGGACTG TCGGGCAGCT CTACAAAGAA TCCCTCACCA AGCTGATGGC AACTCTCCGG 
AACACCAACC CTAACTTCGT CCGCTGCATC ATTCCAAATC ACGAGAAGCG AGCTGGAAAA 
CTGGATCCGC ACCTAGTGCT TGATCAGCTT CGCTGTAACG GCGTTCTGGA AGGAATCAGG 
ATCTGTCGCC AGGGGTTCCC CAACCGGATA GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTAACTC CAAATGCTAT CCCTAAGGGT TTCATGGACG GCAAACAGGC CTGCGAACGA 
ATGATCCGGG CTTTAGAACT GGACCCAAAC TTGTACAGAA TCGGACAGAG CAAGATATTT 
TTCCGAGCTG GCGTGCTGGC ACACTTAGAG GAAGAGAGGG ATCTAAAGAT CACCGACATC 
ATTATCTTCT TCCAGGCTGT CTGCAGAGGC TACCTGGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGCGCCCT GAAGGTCCTG CAGCGGAACT GCGCCGCATA CCTGAAGCTC 
CGGCACTGGC AGTGGTGGCG TGTCTTCACG AAGGTGAAGC CGCTCCTGCA AGTGACCCGC 
CAGGAGGAAG AGCTTCAGGC CAAAGACGAG GAGCTGCTGA AGGTGAAGGA GAGGCAGACG 
AAGGTGGAGG GCGAGCTGGA GGAAATGGAG AGGAAGCACC AGCAGCTCCT GGAAGAGAAG 
AACATCCTAA CAGAGCAGCT CCAAGCAGAG ACAGAGCTCT TTGCTGAGGC AGAAGAGATG 
AGAGCCAGGC TCGCCGCCAA GAAGCAGGAG CTGGAGGAGA TTCTCCATGA CTTGGAATCC 
AGGGTCGAAG AGGAAGAAGA ACGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCTCACATTC AGGACCTAGA GGAGCAACTG GATGAGGAAG AAGGCGCTCG CCAGAAACTA 
CAGCTGGAAA AGGTGACAGC GGAAGCAAAA ATCAAGAAGA TGGAGGAGGA GATCCTGCTG 
CTTGAGGACC AGAATTCCAA ATTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAGTGTTCCT CTCAGCTGGC TGAGGAGGAA GAAAAGGCCA AAAACTTGGC CAAAATCAGG 
AATAAGCAGG AAGTGATGAT CTCAGATTTA GAAGAACGCT TGAAGAAGGA AGAGAAGACG 
CGTCAGGAGC TGGAGAAGGC CAAACGAAAA CTGGACGGTG AGACCACTGA CCTGCAGGAC 
CAGATCGCAG AGCTCCAGGC GCAGATCGAC GAGCTCAAGG TCCAGCTGGC CAAGAAGGAG 
GAGGAGCTGC AAGGCGCGCT GGCCAGGGGT GACGATGAGA CGCTCCATAA GAACAATGCC 
CTCAAGGTGG TGCGAGAGCT GCAAGCCCAA ATCGCCGAGC TTCAGGAGGA CTTTGAATCA 
GAGAAGGCTT CACGGAACAA GGCGGAGAAG CAGAAGAGGG ACCTGAGTGA GGAGCTGGAG 
GCCCTGAAAA CAGAGCTGGA GGACACGCTG GACACCACCG CGGCTCAGCA GGAACTTCGC 
ACGAAGCGTG AACAAGAAGT GGCAGAGCTG AAGAAGGCTC TGGAGGAGGA AACGAGGAGC 
CACGAGGCTC AAATCCAGGA CATGAGACAG CGACACGCCA CAGCCCTGGA GGAGCTGTCG 
GAGCAGCTGG AACAGGCAAA GAGGTTCAAA GCGAATCTGG AGAAGAACAA GCAGGGCCTG 
GAGACTGACA ACAAGGAGCT GGCGTGTGAG GTGAAGGTGC TACAGCAGGT CAAGGCTGAG 
TCTGAGCACA AGAGGAAGAA GCTCGACGCC CAGGTGCAGG AGCTGCACGC CAAGGTCTCA 
GAAGGAGACA GGCTCAGGGT GGAGCTGGCC GAGAAGGCAA ACAAATTACA GAATGAACTG 
GACAATGTCT CAAGTCTTCT GGAAGAAGCA GAGAAGAAGG GCATTAAGTT TGCTAAGGAC 
GCAGCTAGTC TTGAGTCTCA ACTACAGGAC ACTCAGGAGC TCCTTCAGGA GGAGACCCGC 
CAGAAGCTGA ACCTGAGCAG CCGCATCCGG CAGCTGGAGG AGGAGAAGAA CAGTCTCCAG 
GAGCAGCAGG AGGAGGAGGA GGAGGCCAGG AAAAACCTGG AAAAGCAGGT GTTGGTGCTG 
CAGTCCCAGC TCGCCGATAC CAAGAAGAAG GTAGATGATG ACTTAGGAAC CATCGAGAGC 
TTAGAAGAAG CCAGAAAGAA GCTTCTGAAA GATGTGGAGG CCCTGGGCCA GCGCCTGGAG 
GAGAAGGCTC TGGCCTATGA CAAGCTGGAG AAGACCAAGA ACCGCCTGCA GCAGGAGCTC 
GACGACCTGA CAGTGGACCT AGATCACCAG CGCCAGATCG CCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGACCAGCT GCTGGCAGAG GAGAAGAACA TCTCCGCCCG CTATGCAGAA 
GAGCGAGACC GCGCAGAGGC AGAGGCCAGG GAGAAGGAAA CCAAAGCCCT GTCCCTGGCC 
CGAGCCCTCG AGGAGGCCCT AGAAACCAAG GAAGAGTTTG AGAGACAGAA CAAGCAGCTG 
CGGGCAGACA TGGAGGACCT GATAAGCTCC AAGGATGACG TGGGGAAGAA CGTGCACGAG 
CTTGAAAAAT CGAAGCGGGC CCTGGAGCAA CAGGTGGAGG AGATGAGGAC ACAGCTGGAA 
GAGCTGGAGG ATGAGCTTCA GGCCACAGAA GACGCCAAGC TTCGCCTGGA AGTCAACATG 
CAGGCCATGA AGGCCCAGTT TGAGAGGGAC TTACAAACCA GGGACGAGCA GAATGAAGAG 
AAGAAGCGGC TCCTGGTTAA GCAGGTGCGG GAGCTGGAGG CAGAGCTGGA AGATGAGCGG 
AAGCAGCGAG CGCTGGCGGT CGCTTCTAAA AAGAAACTGG AAATAGACCT GAAGGACCTT 
GAAGCCCAGA TTGAGGCTGC GAACAAAGCG AGGGACGAAG TGATCAAGCA GCTTCGCAAG 
CTTCAGGCTC AGATGAAGGA TTACCAACGT GAATTAGAAG AAGCCCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAA AAGAAACTGA AGAGTCTGGA AGCAGAAATC 
CTTCAATTGC AGGAGGAACT GGCCTCATCT GAGCGAGCGC GCCGCCACGC CGAGCAGGAG 
AGAGATGAGC TGGCTGACGA GATCGCCAAC AGCGCCTCTG GCAAGTCTGC GCTGCTGGAT 
GAGAAGCGGC GTCTGGAGGC ACGGATCGCG CAGCTGGAGG AGGAGCTGGA AGAGGAGCAG 
AGCAACATGG AGCTGCTCAA CGACCGCTTC CGCAAGACCA CGCTGCAGGT GGACACGCTG 
AACGCCGAGC TCGCGGCTGA GCGCAGCGCC GCCCAGAAGA GCGACAACGC ACGCCAGCAG 
CTAGAGCGGC AGAACAAGGA GCTGAAGGCC AAGCTGCAGG AACTGGAGGG CGCCGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCCCTG GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTGGAGCAGG AAGCCAAGGA ACGAGCAGCC GCCAACAAAC TAGTCCGTCG CACGGAGAAA 
AAGCTGAAAG AGATCTTCAT GCAGGTTGAA GATGAGCGTC GACATGCGGA TCAGTATAAA 
GAACAGATGG AGAAGGCCAA CGCCAGGATG AAACAGCTCA AGCGGCAGCT GGAGGAGGCA 
GAGGAGGAAG CCACACGTGC CAACGCATCT CGGCGTAAGC TCCAGCGGGA ACTGGACGAT 
GCCACGGAGG CCAACGAGGG CCTGAGCCGT GAGGTCAGCA CGCTGAAGAA CCGGCTGAGA 
CGAGGTGGCC CCATAAGCTT CTCGTCCAGC CGATCGGGCC GGCGCCAGCT ACACATCGAG 
GGAGCCTCCC TGGAGCTGTC AGACGATGAC ACGGAGAGTA AGACCAGTGA CGTCAACGAG 
ACACAGCCGC CGCAGTCGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969069.1
CDS	132..6155
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_014113594.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113594.1

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTCAGGAAT CGCCTAAACC AGTGAAACAT 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC CTTTGGAAAT 
GCGAAGACTG TGAAAAATGA TAATTCATCT CGCTTTGGCA AGTTTATTCG AATCAACTTT 
GATGTAACTG GCTATATTGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAGTT GTTATCTGGA 
GCAGGAGAAC ACCTAAAGTC GGATTTGCTC CTCGAAGGAT TTAATAACTA CAGATTCCTC 
TCCAACGGCT ATATTCCCAT CCCGGGACAG CAAGACAAGG ACAACTTTCA GGAAACCATG 
GAAGCAATGC ACATCATGGG CTTCTCTCAC GAGGAGATTC TGTCAATGCT TAAAGTAGTG 
TCATCAGTGC TACAGTTCGG AAATATTTCT TTCAAAAAGG AGCGAAATAC TGATCAAGCA 
TCCATGCCAG AGAACACAGT TGCACAGAAA CTCTGCCATC TTCTTGGGAT GAACGTGATG 
GAGTTCACGC GGGCCATCCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA TGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCG GTAGAAGCAT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GTTGGCTCGT TCACCGTATC AACAAAGCTC TGGACAGGAC CAAGCGTCAG 
GGCGCATCTT TTATTGGAAT CCTGGATATC GCTGGATTCG AAATATTTGA GCTCAACTCG 
TTCGAGCAAC TGTGCATCAA CTACACCAAT GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAG CTTCATTGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATCGAC CTCATCGAGA GACCCGCTAA CCCCCCTGGT 
GTGCTGGCCC TTTTGGATGA AGAGTGCTGG TTCCCGAAGG CCACGGACAA AACCTTTGTT 
GAAAAACTAG TTCAAGAGCA AGGCTCTCAC TCCAAGTTCC AGAAACCCCG GCAACTGAAA 
GACAGAGCTG ACTTCTGCAT CATCCATTAT GCGGGCAAGG TGGACTACAA GGCTGATGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAC GACAATGTGG CCACCCTCCT GCACCAGTCA 
TCTGACAGAT TCGTGGCAGA GCTTTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGTATCGT GGGTCTGGAT 
CAGGTCACGG GCATGACGGA GACAGCCTTT GGCTCTGCGT ATAAAACCAA GAAGGGCATG 
TTTCGGACTG TCGGGCAGCT CTACAAAGAA TCCCTCACCA AGCTGATGGC AACTCTCCGG 
AACACCAACC CTAACTTCGT CCGCTGCATC ATTCCAAATC ACGAGAAGCG AGCTGGAAAA 
CTGGATCCGC ACCTAGTGCT TGATCAGCTT CGCTGTAACG GCGTTCTGGA AGGAATCAGG 
ATCTGTCGCC AGGGGTTCCC CAACCGGATA GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTAACTC CAAATGCTAT CCCTAAGGGT TTCATGGACG GCAAACAGGC CTGCGAACGA 
ATGATCCGGG CTTTAGAACT GGACCCAAAC TTGTACAGAA TCGGACAGAG CAAGATATTT 
TTCCGAGCTG GCGTGCTGGC ACACTTAGAG GAAGAGAGGG ATCTAAAGAT CACCGACATC 
ATTATCTTCT TCCAGGCTGT CTGCAGAGGC TACCTGGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGCGCCCT GAAGGTCCTG CAGCGGAACT GCGCCGCATA CCTGAAGCTC 
CGGCACTGGC AGTGGTGGCG TGTCTTCACG AAGGTGAAGC CGCTCCTGCA AGTGACCCGC 
CAGGAGGAAG AGCTTCAGGC CAAAGACGAG GAGCTGCTGA AGGTGAAGGA GAGGCAGACG 
AAGGTGGAGG GCGAGCTGGA GGAAATGGAG AGGAAGCACC AGCAGCTCCT GGAAGAGAAG 
AACATCCTAA CAGAGCAGCT CCAAGCAGAG ACAGAGCTCT TTGCTGAGGC AGAAGAGATG 
AGAGCCAGGC TCGCCGCCAA GAAGCAGGAG CTGGAGGAGA TTCTCCATGA CTTGGAATCC 
AGGGTCGAAG AGGAAGAAGA ACGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCTCACATTC AGGACCTAGA GGAGCAACTG GATGAGGAAG AAGGCGCTCG CCAGAAACTA 
CAGCTGGAAA AGGTGACAGC GGAAGCAAAA ATCAAGAAGA TGGAGGAGGA GATCCTGCTG 
CTTGAGGACC AGAATTCCAA ATTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAGTGTTCCT CTCAGCTGGC TGAGGAGGAA GAAAAGGCCA AAAACTTGGC CAAAATCAGG 
AATAAGCAGG AAGTGATGAT CTCAGATTTA GAAGAACGCT TGAAGAAGGA AGAGAAGACG 
CGTCAGGAGC TGGAGAAGGC CAAACGAAAA CTGGACGGTG AGACCACTGA CCTGCAGGAC 
CAGATCGCAG AGCTCCAGGC GCAGATCGAC GAGCTCAAGG TCCAGCTGGC CAAGAAGGAG 
GAGGAGCTGC AAGGCGCGCT GGCCAGGGGT GACGATGAGA CGCTCCATAA GAACAATGCC 
CTCAAGGTGG TGCGAGAGCT GCAAGCCCAA ATCGCCGAGC TTCAGGAGGA CTTTGAATCA 
GAGAAGGCTT CACGGAACAA GGCGGAGAAG CAGAAGAGGG ACCTGAGTGA GGAGCTGGAG 
GCCCTGAAAA CAGAGCTGGA GGACACGCTG GACACCACCG CGGCTCAGCA GGAACTTCGC 
ACGAAGCGTG AACAAGAAGT GGCAGAGCTG AAGAAGGCTC TGGAGGAGGA AACGAGGAGC 
CACGAGGCTC AAATCCAGGA CATGAGACAG CGACACGCCA CAGCCCTGGA GGAGCTGTCG 
GAGCAGCTGG AACAGGCAAA GAGGTTCAAA GCGAATCTGG AGAAGAACAA GCAGGGCCTG 
GAGACTGACA ACAAGGAGCT GGCGTGTGAG GTGAAGGTGC TACAGCAGGT CAAGGCTGAG 
TCTGAGCACA AGAGGAAGAA GCTCGACGCC CAGGTGCAGG AGCTGCACGC CAAGGTCTCA 
GAAGGAGACA GGCTCAGGGT GGAGCTGGCC GAGAAGGCAA ACAAATTACA GAATGAACTG 
GACAATGTCT CAAGTCTTCT GGAAGAAGCA GAGAAGAAGG GCATTAAGTT TGCTAAGGAC 
GCAGCTAGTC TTGAGTCTCA ACTACAGGAC ACTCAGGAGC TCCTTCAGGA GGAGACCCGC 
CAGAAGCTGA ACCTGAGCAG CCGCATCCGG CAGCTGGAGG AGGAGAAGAA CAGTCTCCAG 
GAGCAGCAGG AGGAGGAGGA GGAGGCCAGG AAAAACCTGG AAAAGCAGGT GTTGGTGCTG 
CAGTCCCAGC TCGCCGATAC CAAGAAGAAG GTAGATGATG ACTTAGGAAC CATCGAGAGC 
TTAGAAGAAG CCAGAAAGAA GCTTCTGAAA GATGTGGAGG CCCTGGGCCA GCGCCTGGAG 
GAGAAGGCTC TGGCCTATGA CAAGCTGGAG AAGACCAAGA ACCGCCTGCA GCAGGAGCTC 
GACGACCTGA CAGTGGACCT AGATCACCAG CGCCAGATCG CCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGACCAGCT GCTGGCAGAG GAGAAGAACA TCTCCGCCCG CTATGCAGAA 
GAGCGAGACC GCGCAGAGGC AGAGGCCAGG GAGAAGGAAA CCAAAGCCCT GTCCCTGGCC 
CGAGCCCTCG AGGAGGCCCT AGAAACCAAG GAAGAGTTTG AGAGACAGAA CAAGCAGCTG 
CGGGCAGACA TGGAGGACCT GATAAGCTCC AAGGATGACG TGGGGAAGAA CGTGCACGAG 
CTTGAAAAAT CGAAGCGGGC CCTGGAGCAA CAGGTGGAGG AGATGAGGAC ACAGCTGGAA 
GAGCTGGAGG ATGAGCTTCA GGCCACAGAA GACGCCAAGC TTCGCCTGGA AGTCAACATG 
CAGGCCATGA AGGCCCAGTT TGAGAGGGAC TTACAAACCA GGGACGAGCA GAATGAAGAG 
AAGAAGCGGC TCCTGGTTAA GCAGGTGCGG GAGCTGGAGG CAGAGCTGGA AGATGAGCGG 
AAGCAGCGAG CGCTGGCGGT CGCTTCTAAA AAGAAACTGG AAATAGACCT GAAGGACCTT 
GAAGCCCAGA TTGAGGCTGC GAACAAAGCG AGGGACGAAG TGATCAAGCA GCTTCGCAAG 
CTTCAGGCTC AGATGAAGGA TTACCAACGT GAATTAGAAG AAGCCCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAA AAGAAACTGA AGAGTCTGGA AGCAGAAATC 
CTTCAATTGC AGGAGGAACT GGCCTCATCT GAGCGAGCGC GCCGCCACGC CGAGCAGGAG 
AGAGATGAGC TGGCTGACGA GATCGCCAAC AGCGCCTCTG GCAAGTCTGC GCTGCTGGAT 
GAGAAGCGGC GTCTGGAGGC ACGGATCGCG CAGCTGGAGG AGGAGCTGGA AGAGGAGCAG 
AGCAACATGG AGCTGCTCAA CGACCGCTTC CGCAAGACCA CGCTGCAGGT GGACACGCTG 
AACGCCGAGC TCGCGGCTGA GCGCAGCGCC GCCCAGAAGA GCGACAACGC ACGCCAGCAG 
CTAGAGCGGC AGAACAAGGA GCTGAAGGCC AAGCTGCAGG AACTGGAGGG CGCCGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCCCTG GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTGGAGCAGG AAGCCAAGGA ACGAGCAGCC GCCAACAAAC TAGTCCGTCG CACGGAGAAA 
AAGCTGAAAG AGATCTTCAT GCAGGTTGAA GATGAGCGTC GACATGCGGA TCAGTATAAA 
GAACAGATGG AGAAGGCCAA CGCCAGGATG AAACAGCTCA AGCGGCAGCT GGAGGAGGCA 
GAGGAGGAAG CCACACGTGC CAACGCATCT CGGCGTAAGC TCCAGCGGGA ACTGGACGAT 
GCCACGGAGG CCAACGAGGG CCTGAGCCGT GAGGTCAGCA CGCTGAAGAA CCGGCTGAGA 
CGAGGTGGCC CCATAAGCTT CTCGTCCAGC CGATCGGGCC GGCGCCAGCT ACACATCGAG 
GGAGCCTCCC TGGAGCTGTC AGACGATGAC ACGGAGAGTA AGACCAGTGA CGTCAACGAG 
ACACAGCCGC CGCAGTCGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_014113594.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969069.1 Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X1, mRNA.

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CloneID	OCe36456
Clone ID Related Accession (Same CDS sequence)	XM_005620039.3 , XM_014113594.1 , XM_014113594.2
Accession Version	XM_014113594.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	myosin-10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113594.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTCAGGAAT CGCCTAAACC AGTGAAACAT 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC CTTTGGAAAT 
GCGAAGACTG TGAAAAATGA TAATTCATCT CGCTTTGGCA AGTTTATTCG AATCAACTTT 
GATGTAACTG GCTATATTGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAGTT GTTATCTGGA 
GCAGGAGAAC ACCTAAAGTC GGATTTGCTC CTCGAAGGAT TTAATAACTA CAGATTCCTC 
TCCAACGGCT ATATTCCCAT CCCGGGACAG CAAGACAAGG ACAACTTTCA GGAAACCATG 
GAAGCAATGC ACATCATGGG CTTCTCTCAC GAGGAGATTC TGTCAATGCT TAAAGTAGTG 
TCATCAGTGC TACAGTTCGG AAATATTTCT TTCAAAAAGG AGCGAAATAC TGATCAAGCA 
TCCATGCCAG AGAACACAGT TGCACAGAAA CTCTGCCATC TTCTTGGGAT GAACGTGATG 
GAGTTCACGC GGGCCATCCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA TGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCG GTAGAAGCAT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GTTGGCTCGT TCACCGTATC AACAAAGCTC TGGACAGGAC CAAGCGTCAG 
GGCGCATCTT TTATTGGAAT CCTGGATATC GCTGGATTCG AAATATTTGA GCTCAACTCG 
TTCGAGCAAC TGTGCATCAA CTACACCAAT GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAG CTTCATTGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATCGAC CTCATCGAGA GACCCGCTAA CCCCCCTGGT 
GTGCTGGCCC TTTTGGATGA AGAGTGCTGG TTCCCGAAGG CCACGGACAA AACCTTTGTT 
GAAAAACTAG TTCAAGAGCA AGGCTCTCAC TCCAAGTTCC AGAAACCCCG GCAACTGAAA 
GACAGAGCTG ACTTCTGCAT CATCCATTAT GCGGGCAAGG TGGACTACAA GGCTGATGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAC GACAATGTGG CCACCCTCCT GCACCAGTCA 
TCTGACAGAT TCGTGGCAGA GCTTTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGTATCGT GGGTCTGGAT 
CAGGTCACGG GCATGACGGA GACAGCCTTT GGCTCTGCGT ATAAAACCAA GAAGGGCATG 
TTTCGGACTG TCGGGCAGCT CTACAAAGAA TCCCTCACCA AGCTGATGGC AACTCTCCGG 
AACACCAACC CTAACTTCGT CCGCTGCATC ATTCCAAATC ACGAGAAGCG AGCTGGAAAA 
CTGGATCCGC ACCTAGTGCT TGATCAGCTT CGCTGTAACG GCGTTCTGGA AGGAATCAGG 
ATCTGTCGCC AGGGGTTCCC CAACCGGATA GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTAACTC CAAATGCTAT CCCTAAGGGT TTCATGGACG GCAAACAGGC CTGCGAACGA 
ATGATCCGGG CTTTAGAACT GGACCCAAAC TTGTACAGAA TCGGACAGAG CAAGATATTT 
TTCCGAGCTG GCGTGCTGGC ACACTTAGAG GAAGAGAGGG ATCTAAAGAT CACCGACATC 
ATTATCTTCT TCCAGGCTGT CTGCAGAGGC TACCTGGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGCGCCCT GAAGGTCCTG CAGCGGAACT GCGCCGCATA CCTGAAGCTC 
CGGCACTGGC AGTGGTGGCG TGTCTTCACG AAGGTGAAGC CGCTCCTGCA AGTGACCCGC 
CAGGAGGAAG AGCTTCAGGC CAAAGACGAG GAGCTGCTGA AGGTGAAGGA GAGGCAGACG 
AAGGTGGAGG GCGAGCTGGA GGAAATGGAG AGGAAGCACC AGCAGCTCCT GGAAGAGAAG 
AACATCCTAA CAGAGCAGCT CCAAGCAGAG ACAGAGCTCT TTGCTGAGGC AGAAGAGATG 
AGAGCCAGGC TCGCCGCCAA GAAGCAGGAG CTGGAGGAGA TTCTCCATGA CTTGGAATCC 
AGGGTCGAAG AGGAAGAAGA ACGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCTCACATTC AGGACCTAGA GGAGCAACTG GATGAGGAAG AAGGCGCTCG CCAGAAACTA 
CAGCTGGAAA AGGTGACAGC GGAAGCAAAA ATCAAGAAGA TGGAGGAGGA GATCCTGCTG 
CTTGAGGACC AGAATTCCAA ATTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAGTGTTCCT CTCAGCTGGC TGAGGAGGAA GAAAAGGCCA AAAACTTGGC CAAAATCAGG 
AATAAGCAGG AAGTGATGAT CTCAGATTTA GAAGAACGCT TGAAGAAGGA AGAGAAGACG 
CGTCAGGAGC TGGAGAAGGC CAAACGAAAA CTGGACGGTG AGACCACTGA CCTGCAGGAC 
CAGATCGCAG AGCTCCAGGC GCAGATCGAC GAGCTCAAGG TCCAGCTGGC CAAGAAGGAG 
GAGGAGCTGC AAGGCGCGCT GGCCAGGGGT GACGATGAGA CGCTCCATAA GAACAATGCC 
CTCAAGGTGG TGCGAGAGCT GCAAGCCCAA ATCGCCGAGC TTCAGGAGGA CTTTGAATCA 
GAGAAGGCTT CACGGAACAA GGCGGAGAAG CAGAAGAGGG ACCTGAGTGA GGAGCTGGAG 
GCCCTGAAAA CAGAGCTGGA GGACACGCTG GACACCACCG CGGCTCAGCA GGAACTTCGC 
ACGAAGCGTG AACAAGAAGT GGCAGAGCTG AAGAAGGCTC TGGAGGAGGA AACGAGGAGC 
CACGAGGCTC AAATCCAGGA CATGAGACAG CGACACGCCA CAGCCCTGGA GGAGCTGTCG 
GAGCAGCTGG AACAGGCAAA GAGGTTCAAA GCGAATCTGG AGAAGAACAA GCAGGGCCTG 
GAGACTGACA ACAAGGAGCT GGCGTGTGAG GTGAAGGTGC TACAGCAGGT CAAGGCTGAG 
TCTGAGCACA AGAGGAAGAA GCTCGACGCC CAGGTGCAGG AGCTGCACGC CAAGGTCTCA 
GAAGGAGACA GGCTCAGGGT GGAGCTGGCC GAGAAGGCAA ACAAATTACA GAATGAACTG 
GACAATGTCT CAAGTCTTCT GGAAGAAGCA GAGAAGAAGG GCATTAAGTT TGCTAAGGAC 
GCAGCTAGTC TTGAGTCTCA ACTACAGGAC ACTCAGGAGC TCCTTCAGGA GGAGACCCGC 
CAGAAGCTGA ACCTGAGCAG CCGCATCCGG CAGCTGGAGG AGGAGAAGAA CAGTCTCCAG 
GAGCAGCAGG AGGAGGAGGA GGAGGCCAGG AAAAACCTGG AAAAGCAGGT GTTGGTGCTG 
CAGTCCCAGC TCGCCGATAC CAAGAAGAAG GTAGATGATG ACTTAGGAAC CATCGAGAGC 
TTAGAAGAAG CCAGAAAGAA GCTTCTGAAA GATGTGGAGG CCCTGGGCCA GCGCCTGGAG 
GAGAAGGCTC TGGCCTATGA CAAGCTGGAG AAGACCAAGA ACCGCCTGCA GCAGGAGCTC 
GACGACCTGA CAGTGGACCT AGATCACCAG CGCCAGATCG CCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGACCAGCT GCTGGCAGAG GAGAAGAACA TCTCCGCCCG CTATGCAGAA 
GAGCGAGACC GCGCAGAGGC AGAGGCCAGG GAGAAGGAAA CCAAAGCCCT GTCCCTGGCC 
CGAGCCCTCG AGGAGGCCCT AGAAACCAAG GAAGAGTTTG AGAGACAGAA CAAGCAGCTG 
CGGGCAGACA TGGAGGACCT GATAAGCTCC AAGGATGACG TGGGGAAGAA CGTGCACGAG 
CTTGAAAAAT CGAAGCGGGC CCTGGAGCAA CAGGTGGAGG AGATGAGGAC ACAGCTGGAA 
GAGCTGGAGG ATGAGCTTCA GGCCACAGAA GACGCCAAGC TTCGCCTGGA AGTCAACATG 
CAGGCCATGA AGGCCCAGTT TGAGAGGGAC TTACAAACCA GGGACGAGCA GAATGAAGAG 
AAGAAGCGGC TCCTGGTTAA GCAGGTGCGG GAGCTGGAGG CAGAGCTGGA AGATGAGCGG 
AAGCAGCGAG CGCTGGCGGT CGCTTCTAAA AAGAAACTGG AAATAGACCT GAAGGACCTT 
GAAGCCCAGA TTGAGGCTGC GAACAAAGCG AGGGACGAAG TGATCAAGCA GCTTCGCAAG 
CTTCAGGCTC AGATGAAGGA TTACCAACGT GAATTAGAAG AAGCCCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAA AAGAAACTGA AGAGTCTGGA AGCAGAAATC 
CTTCAATTGC AGGAGGAACT GGCCTCATCT GAGCGAGCGC GCCGCCACGC CGAGCAGGAG 
AGAGATGAGC TGGCTGACGA GATCGCCAAC AGCGCCTCTG GCAAGTCTGC GCTGCTGGAT 
GAGAAGCGGC GTCTGGAGGC ACGGATCGCG CAGCTGGAGG AGGAGCTGGA AGAGGAGCAG 
AGCAACATGG AGCTGCTCAA CGACCGCTTC CGCAAGACCA CGCTGCAGGT GGACACGCTG 
AACGCCGAGC TCGCGGCTGA GCGCAGCGCC GCCCAGAAGA GCGACAACGC ACGCCAGCAG 
CTAGAGCGGC AGAACAAGGA GCTGAAGGCC AAGCTGCAGG AACTGGAGGG CGCCGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCCCTG GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTGGAGCAGG AAGCCAAGGA ACGAGCAGCC GCCAACAAAC TAGTCCGTCG CACGGAGAAA 
AAGCTGAAAG AGATCTTCAT GCAGGTTGAA GATGAGCGTC GACATGCGGA TCAGTATAAA 
GAACAGATGG AGAAGGCCAA CGCCAGGATG AAACAGCTCA AGCGGCAGCT GGAGGAGGCA 
GAGGAGGAAG CCACACGTGC CAACGCATCT CGGCGTAAGC TCCAGCGGGA ACTGGACGAT 
GCCACGGAGG CCAACGAGGG CCTGAGCCGT GAGGTCAGCA CGCTGAAGAA CCGGCTGAGA 
CGAGGTGGCC CCATAAGCTT CTCGTCCAGC CGATCGGGCC GGCGCCAGCT ACACATCGAG 
GGAGCCTCCC TGGAGCTGTC AGACGATGAC ACGGAGAGTA AGACCAGTGA CGTCAACGAG 
ACACAGCCGC CGCAGTCGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969069.1
CDS	192..6215
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_014113594.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113594.2

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTCAGGAAT CGCCTAAACC AGTGAAACAT 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC CTTTGGAAAT 
GCGAAGACTG TGAAAAATGA TAATTCATCT CGCTTTGGCA AGTTTATTCG AATCAACTTT 
GATGTAACTG GCTATATTGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAGTT GTTATCTGGA 
GCAGGAGAAC ACCTAAAGTC GGATTTGCTC CTCGAAGGAT TTAATAACTA CAGATTCCTC 
TCCAACGGCT ATATTCCCAT CCCGGGACAG CAAGACAAGG ACAACTTTCA GGAAACCATG 
GAAGCAATGC ACATCATGGG CTTCTCTCAC GAGGAGATTC TGTCAATGCT TAAAGTAGTG 
TCATCAGTGC TACAGTTCGG AAATATTTCT TTCAAAAAGG AGCGAAATAC TGATCAAGCA 
TCCATGCCAG AGAACACAGT TGCACAGAAA CTCTGCCATC TTCTTGGGAT GAACGTGATG 
GAGTTCACGC GGGCCATCCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA TGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCG GTAGAAGCAT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GTTGGCTCGT TCACCGTATC AACAAAGCTC TGGACAGGAC CAAGCGTCAG 
GGCGCATCTT TTATTGGAAT CCTGGATATC GCTGGATTCG AAATATTTGA GCTCAACTCG 
TTCGAGCAAC TGTGCATCAA CTACACCAAT GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAG CTTCATTGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATCGAC CTCATCGAGA GACCCGCTAA CCCCCCTGGT 
GTGCTGGCCC TTTTGGATGA AGAGTGCTGG TTCCCGAAGG CCACGGACAA AACCTTTGTT 
GAAAAACTAG TTCAAGAGCA AGGCTCTCAC TCCAAGTTCC AGAAACCCCG GCAACTGAAA 
GACAGAGCTG ACTTCTGCAT CATCCATTAT GCGGGCAAGG TGGACTACAA GGCTGATGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAC GACAATGTGG CCACCCTCCT GCACCAGTCA 
TCTGACAGAT TCGTGGCAGA GCTTTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGTATCGT GGGTCTGGAT 
CAGGTCACGG GCATGACGGA GACAGCCTTT GGCTCTGCGT ATAAAACCAA GAAGGGCATG 
TTTCGGACTG TCGGGCAGCT CTACAAAGAA TCCCTCACCA AGCTGATGGC AACTCTCCGG 
AACACCAACC CTAACTTCGT CCGCTGCATC ATTCCAAATC ACGAGAAGCG AGCTGGAAAA 
CTGGATCCGC ACCTAGTGCT TGATCAGCTT CGCTGTAACG GCGTTCTGGA AGGAATCAGG 
ATCTGTCGCC AGGGGTTCCC CAACCGGATA GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTAACTC CAAATGCTAT CCCTAAGGGT TTCATGGACG GCAAACAGGC CTGCGAACGA 
ATGATCCGGG CTTTAGAACT GGACCCAAAC TTGTACAGAA TCGGACAGAG CAAGATATTT 
TTCCGAGCTG GCGTGCTGGC ACACTTAGAG GAAGAGAGGG ATCTAAAGAT CACCGACATC 
ATTATCTTCT TCCAGGCTGT CTGCAGAGGC TACCTGGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGCGCCCT GAAGGTCCTG CAGCGGAACT GCGCCGCATA CCTGAAGCTC 
CGGCACTGGC AGTGGTGGCG TGTCTTCACG AAGGTGAAGC CGCTCCTGCA AGTGACCCGC 
CAGGAGGAAG AGCTTCAGGC CAAAGACGAG GAGCTGCTGA AGGTGAAGGA GAGGCAGACG 
AAGGTGGAGG GCGAGCTGGA GGAAATGGAG AGGAAGCACC AGCAGCTCCT GGAAGAGAAG 
AACATCCTAA CAGAGCAGCT CCAAGCAGAG ACAGAGCTCT TTGCTGAGGC AGAAGAGATG 
AGAGCCAGGC TCGCCGCCAA GAAGCAGGAG CTGGAGGAGA TTCTCCATGA CTTGGAATCC 
AGGGTCGAAG AGGAAGAAGA ACGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCTCACATTC AGGACCTAGA GGAGCAACTG GATGAGGAAG AAGGCGCTCG CCAGAAACTA 
CAGCTGGAAA AGGTGACAGC GGAAGCAAAA ATCAAGAAGA TGGAGGAGGA GATCCTGCTG 
CTTGAGGACC AGAATTCCAA ATTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAGTGTTCCT CTCAGCTGGC TGAGGAGGAA GAAAAGGCCA AAAACTTGGC CAAAATCAGG 
AATAAGCAGG AAGTGATGAT CTCAGATTTA GAAGAACGCT TGAAGAAGGA AGAGAAGACG 
CGTCAGGAGC TGGAGAAGGC CAAACGAAAA CTGGACGGTG AGACCACTGA CCTGCAGGAC 
CAGATCGCAG AGCTCCAGGC GCAGATCGAC GAGCTCAAGG TCCAGCTGGC CAAGAAGGAG 
GAGGAGCTGC AAGGCGCGCT GGCCAGGGGT GACGATGAGA CGCTCCATAA GAACAATGCC 
CTCAAGGTGG TGCGAGAGCT GCAAGCCCAA ATCGCCGAGC TTCAGGAGGA CTTTGAATCA 
GAGAAGGCTT CACGGAACAA GGCGGAGAAG CAGAAGAGGG ACCTGAGTGA GGAGCTGGAG 
GCCCTGAAAA CAGAGCTGGA GGACACGCTG GACACCACCG CGGCTCAGCA GGAACTTCGC 
ACGAAGCGTG AACAAGAAGT GGCAGAGCTG AAGAAGGCTC TGGAGGAGGA AACGAGGAGC 
CACGAGGCTC AAATCCAGGA CATGAGACAG CGACACGCCA CAGCCCTGGA GGAGCTGTCG 
GAGCAGCTGG AACAGGCAAA GAGGTTCAAA GCGAATCTGG AGAAGAACAA GCAGGGCCTG 
GAGACTGACA ACAAGGAGCT GGCGTGTGAG GTGAAGGTGC TACAGCAGGT CAAGGCTGAG 
TCTGAGCACA AGAGGAAGAA GCTCGACGCC CAGGTGCAGG AGCTGCACGC CAAGGTCTCA 
GAAGGAGACA GGCTCAGGGT GGAGCTGGCC GAGAAGGCAA ACAAATTACA GAATGAACTG 
GACAATGTCT CAAGTCTTCT GGAAGAAGCA GAGAAGAAGG GCATTAAGTT TGCTAAGGAC 
GCAGCTAGTC TTGAGTCTCA ACTACAGGAC ACTCAGGAGC TCCTTCAGGA GGAGACCCGC 
CAGAAGCTGA ACCTGAGCAG CCGCATCCGG CAGCTGGAGG AGGAGAAGAA CAGTCTCCAG 
GAGCAGCAGG AGGAGGAGGA GGAGGCCAGG AAAAACCTGG AAAAGCAGGT GTTGGTGCTG 
CAGTCCCAGC TCGCCGATAC CAAGAAGAAG GTAGATGATG ACTTAGGAAC CATCGAGAGC 
TTAGAAGAAG CCAGAAAGAA GCTTCTGAAA GATGTGGAGG CCCTGGGCCA GCGCCTGGAG 
GAGAAGGCTC TGGCCTATGA CAAGCTGGAG AAGACCAAGA ACCGCCTGCA GCAGGAGCTC 
GACGACCTGA CAGTGGACCT AGATCACCAG CGCCAGATCG CCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGACCAGCT GCTGGCAGAG GAGAAGAACA TCTCCGCCCG CTATGCAGAA 
GAGCGAGACC GCGCAGAGGC AGAGGCCAGG GAGAAGGAAA CCAAAGCCCT GTCCCTGGCC 
CGAGCCCTCG AGGAGGCCCT AGAAACCAAG GAAGAGTTTG AGAGACAGAA CAAGCAGCTG 
CGGGCAGACA TGGAGGACCT GATAAGCTCC AAGGATGACG TGGGGAAGAA CGTGCACGAG 
CTTGAAAAAT CGAAGCGGGC CCTGGAGCAA CAGGTGGAGG AGATGAGGAC ACAGCTGGAA 
GAGCTGGAGG ATGAGCTTCA GGCCACAGAA GACGCCAAGC TTCGCCTGGA AGTCAACATG 
CAGGCCATGA AGGCCCAGTT TGAGAGGGAC TTACAAACCA GGGACGAGCA GAATGAAGAG 
AAGAAGCGGC TCCTGGTTAA GCAGGTGCGG GAGCTGGAGG CAGAGCTGGA AGATGAGCGG 
AAGCAGCGAG CGCTGGCGGT CGCTTCTAAA AAGAAACTGG AAATAGACCT GAAGGACCTT 
GAAGCCCAGA TTGAGGCTGC GAACAAAGCG AGGGACGAAG TGATCAAGCA GCTTCGCAAG 
CTTCAGGCTC AGATGAAGGA TTACCAACGT GAATTAGAAG AAGCCCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAA AAGAAACTGA AGAGTCTGGA AGCAGAAATC 
CTTCAATTGC AGGAGGAACT GGCCTCATCT GAGCGAGCGC GCCGCCACGC CGAGCAGGAG 
AGAGATGAGC TGGCTGACGA GATCGCCAAC AGCGCCTCTG GCAAGTCTGC GCTGCTGGAT 
GAGAAGCGGC GTCTGGAGGC ACGGATCGCG CAGCTGGAGG AGGAGCTGGA AGAGGAGCAG 
AGCAACATGG AGCTGCTCAA CGACCGCTTC CGCAAGACCA CGCTGCAGGT GGACACGCTG 
AACGCCGAGC TCGCGGCTGA GCGCAGCGCC GCCCAGAAGA GCGACAACGC ACGCCAGCAG 
CTAGAGCGGC AGAACAAGGA GCTGAAGGCC AAGCTGCAGG AACTGGAGGG CGCCGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCCCTG GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTGGAGCAGG AAGCCAAGGA ACGAGCAGCC GCCAACAAAC TAGTCCGTCG CACGGAGAAA 
AAGCTGAAAG AGATCTTCAT GCAGGTTGAA GATGAGCGTC GACATGCGGA TCAGTATAAA 
GAACAGATGG AGAAGGCCAA CGCCAGGATG AAACAGCTCA AGCGGCAGCT GGAGGAGGCA 
GAGGAGGAAG CCACACGTGC CAACGCATCT CGGCGTAAGC TCCAGCGGGA ACTGGACGAT 
GCCACGGAGG CCAACGAGGG CCTGAGCCGT GAGGTCAGCA CGCTGAAGAA CCGGCTGAGA 
CGAGGTGGCC CCATAAGCTT CTCGTCCAGC CGATCGGGCC GGCGCCAGCT ACACATCGAG 
GGAGCCTCCC TGGAGCTGTC AGACGATGAC ACGGAGAGTA AGACCAGTGA CGTCAACGAG 
ACACAGCCGC CGCAGTCGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_014113595.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969070.1 Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X2, mRNA.

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CloneID	OCe36457
Clone ID Related Accession (Same CDS sequence)	XM_014113595.1 , XM_014113595.2
Accession Version	XM_014113595.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	myosin-10 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGAATCGC CTAAACCAGT GAAACATCAG 
GGGGAACTTG AACGGCAGCT TTTGCAAGCA AATCCAATCC TGGAATCCTT TGGAAATGCG 
AAGACTGTGA AAAATGATAA TTCATCTCGC TTTGGCAAGT TTATTCGAAT CAACTTTGAT 
GTAACTGGCT ATATTGTTGG GGCCAACATT GAAACATACC TTCTGGAAAA ATCTCGTGCT 
GTTCGTCAAG CAAAAGATGA GCGTACTTTT CATATCTTTT ACCAGTTGTT ATCTGGAGCA 
GGAGAACACC TAAAGTCGGA TTTGCTCCTC GAAGGATTTA ATAACTACAG ATTCCTCTCC 
AACGGCTATA TTCCCATCCC GGGACAGCAA GACAAGGACA ACTTTCAGGA AACCATGGAA 
GCAATGCACA TCATGGGCTT CTCTCACGAG GAGATTCTGT CAATGCTTAA AGTAGTGTCA 
TCAGTGCTAC AGTTCGGAAA TATTTCTTTC AAAAAGGAGC GAAATACTGA TCAAGCATCC 
ATGCCAGAGA ACACAGTTGC ACAGAAACTC TGCCATCTTC TTGGGATGAA CGTGATGGAG 
TTCACGCGGG CCATCCTCAC CCCCCGGATC AAGGTCGGCC GAGACTATGT GCAGAAAGCC 
CAGACCAAAG AACAAGCAGA TTTTGCGGTA GAAGCATTGG CAAAAGCTAC CTATGAGCGG 
CTCTTCCGTT GGCTCGTTCA CCGTATCAAC AAAGCTCTGG ACAGGACCAA GCGTCAGGGC 
GCATCTTTTA TTGGAATCCT GGATATCGCT GGATTCGAAA TATTTGAGCT CAACTCGTTC 
GAGCAACTGT GCATCAACTA CACCAATGAG AAGCTGCAGC AGCTCTTCAA CCACACCATG 
TTCATCCTGG AGCAGGAGGA GTACCAGCGC GAGGGCATCG AGTGGAGCTT CATTGACTTT 
GGCCTCGACC TGCAGCCCTG CATCGACCTC ATCGAGAGAC CCGCTAACCC CCCTGGTGTG 
CTGGCCCTTT TGGATGAAGA GTGCTGGTTC CCGAAGGCCA CGGACAAAAC CTTTGTTGAA 
AAACTAGTTC AAGAGCAAGG CTCTCACTCC AAGTTCCAGA AACCCCGGCA ACTGAAAGAC 
AGAGCTGACT TCTGCATCAT CCATTATGCG GGCAAGGTGG ACTACAAGGC TGATGAGTGG 
CTGATGAAGA ACATGGACCC CCTGAACGAC AATGTGGCCA CCCTCCTGCA CCAGTCATCT 
GACAGATTCG TGGCAGAGCT TTGGAAGGAT GAGATTCAGA ATATTCAGAG AGCTTCTTTC 
TATGACAGTG TTTCTGGTCT TCATGAGCCA CCAGTGGACC GTATCGTGGG TCTGGATCAG 
GTCACGGGCA TGACGGAGAC AGCCTTTGGC TCTGCGTATA AAACCAAGAA GGGCATGTTT 
CGGACTGTCG GGCAGCTCTA CAAAGAATCC CTCACCAAGC TGATGGCAAC TCTCCGGAAC 
ACCAACCCTA ACTTCGTCCG CTGCATCATT CCAAATCACG AGAAGCGAGC TGGAAAACTG 
GATCCGCACC TAGTGCTTGA TCAGCTTCGC TGTAACGGCG TTCTGGAAGG AATCAGGATC 
TGTCGCCAGG GGTTCCCCAA CCGGATAGTT TTCCAGGAAT TCAGACAGAG ATACGAGATC 
CTAACTCCAA ATGCTATCCC TAAGGGTTTC ATGGACGGCA AACAGGCCTG CGAACGAATG 
ATCCGGGCTT TAGAACTGGA CCCAAACTTG TACAGAATCG GACAGAGCAA GATATTTTTC 
CGAGCTGGCG TGCTGGCACA CTTAGAGGAA GAGAGGGATC TAAAGATCAC CGACATCATT 
ATCTTCTTCC AGGCTGTCTG CAGAGGCTAC CTGGCCAGAA AGGCCTTCGC CAAGAAGCAG 
CAGCAGCTGA GCGCCCTGAA GGTCCTGCAG CGGAACTGCG CCGCATACCT GAAGCTCCGG 
CACTGGCAGT GGTGGCGTGT CTTCACGAAG GTGAAGCCGC TCCTGCAAGT GACCCGCCAG 
GAGGAAGAGC TTCAGGCCAA AGACGAGGAG CTGCTGAAGG TGAAGGAGAG GCAGACGAAG 
GTGGAGGGCG AGCTGGAGGA AATGGAGAGG AAGCACCAGC AGCTCCTGGA AGAGAAGAAC 
ATCCTAACAG AGCAGCTCCA AGCAGAGACA GAGCTCTTTG CTGAGGCAGA AGAGATGAGA 
GCCAGGCTCG CCGCCAAGAA GCAGGAGCTG GAGGAGATTC TCCATGACTT GGAATCCAGG 
GTCGAAGAGG AAGAAGAACG AAACCAAATC CTCCAAAATG AAAAGAAGAA AATGCAAGCT 
CACATTCAGG ACCTAGAGGA GCAACTGGAT GAGGAAGAAG GCGCTCGCCA GAAACTACAG 
CTGGAAAAGG TGACAGCGGA AGCAAAAATC AAGAAGATGG AGGAGGAGAT CCTGCTGCTT 
GAGGACCAGA ATTCCAAATT TATCAAAGAA AAGAAACTCA TGGAAGATCG CATTGCAGAG 
TGTTCCTCTC AGCTGGCTGA GGAGGAAGAA AAGGCCAAAA ACTTGGCCAA AATCAGGAAT 
AAGCAGGAAG TGATGATCTC AGATTTAGAA GAACGCTTGA AGAAGGAAGA GAAGACGCGT 
CAGGAGCTGG AGAAGGCCAA ACGAAAACTG GACGGTGAGA CCACTGACCT GCAGGACCAG 
ATCGCAGAGC TCCAGGCGCA GATCGACGAG CTCAAGGTCC AGCTGGCCAA GAAGGAGGAG 
GAGCTGCAAG GCGCGCTGGC CAGGGGTGAC GATGAGACGC TCCATAAGAA CAATGCCCTC 
AAGGTGGTGC GAGAGCTGCA AGCCCAAATC GCCGAGCTTC AGGAGGACTT TGAATCAGAG 
AAGGCTTCAC GGAACAAGGC GGAGAAGCAG AAGAGGGACC TGAGTGAGGA GCTGGAGGCC 
CTGAAAACAG AGCTGGAGGA CACGCTGGAC ACCACCGCGG CTCAGCAGGA ACTTCGCACG 
AAGCGTGAAC AAGAAGTGGC AGAGCTGAAG AAGGCTCTGG AGGAGGAAAC GAGGAGCCAC 
GAGGCTCAAA TCCAGGACAT GAGACAGCGA CACGCCACAG CCCTGGAGGA GCTGTCGGAG 
CAGCTGGAAC AGGCAAAGAG GTTCAAAGCG AATCTGGAGA AGAACAAGCA GGGCCTGGAG 
ACTGACAACA AGGAGCTGGC GTGTGAGGTG AAGGTGCTAC AGCAGGTCAA GGCTGAGTCT 
GAGCACAAGA GGAAGAAGCT CGACGCCCAG GTGCAGGAGC TGCACGCCAA GGTCTCAGAA 
GGAGACAGGC TCAGGGTGGA GCTGGCCGAG AAGGCAAACA AATTACAGAA TGAACTGGAC 
AATGTCTCAA GTCTTCTGGA AGAAGCAGAG AAGAAGGGCA TTAAGTTTGC TAAGGACGCA 
GCTAGTCTTG AGTCTCAACT ACAGGACACT CAGGAGCTCC TTCAGGAGGA GACCCGCCAG 
AAGCTGAACC TGAGCAGCCG CATCCGGCAG CTGGAGGAGG AGAAGAACAG TCTCCAGGAG 
CAGCAGGAGG AGGAGGAGGA GGCCAGGAAA AACCTGGAAA AGCAGGTGTT GGTGCTGCAG 
TCCCAGCTCG CCGATACCAA GAAGAAGGTA GATGATGACT TAGGAACCAT CGAGAGCTTA 
GAAGAAGCCA GAAAGAAGCT TCTGAAAGAT GTGGAGGCCC TGGGCCAGCG CCTGGAGGAG 
AAGGCTCTGG CCTATGACAA GCTGGAGAAG ACCAAGAACC GCCTGCAGCA GGAGCTCGAC 
GACCTGACAG TGGACCTAGA TCACCAGCGC CAGATCGCCT CCAACTTGGA GAAGAAGCAG 
AAGAAGTTTG ACCAGCTGCT GGCAGAGGAG AAGAACATCT CCGCCCGCTA TGCAGAAGAG 
CGAGACCGCG CAGAGGCAGA GGCCAGGGAG AAGGAAACCA AAGCCCTGTC CCTGGCCCGA 
GCCCTCGAGG AGGCCCTAGA AACCAAGGAA GAGTTTGAGA GACAGAACAA GCAGCTGCGG 
GCAGACATGG AGGACCTGAT AAGCTCCAAG GATGACGTGG GGAAGAACGT GCACGAGCTT 
GAAAAATCGA AGCGGGCCCT GGAGCAACAG GTGGAGGAGA TGAGGACACA GCTGGAAGAG 
CTGGAGGATG AGCTTCAGGC CACAGAAGAC GCCAAGCTTC GCCTGGAAGT CAACATGCAG 
GCCATGAAGG CCCAGTTTGA GAGGGACTTA CAAACCAGGG ACGAGCAGAA TGAAGAGAAG 
AAGCGGCTCC TGGTTAAGCA GGTGCGGGAG CTGGAGGCAG AGCTGGAAGA TGAGCGGAAG 
CAGCGAGCGC TGGCGGTCGC TTCTAAAAAG AAACTGGAAA TAGACCTGAA GGACCTTGAA 
GCCCAGATTG AGGCTGCGAA CAAAGCGAGG GACGAAGTGA TCAAGCAGCT TCGCAAGCTT 
CAGGCTCAGA TGAAGGATTA CCAACGTGAA TTAGAAGAAG CCCGTGCATC CCGGGATGAG 
ATTTTTGCTC AATCCAAAGA GAGTGAAAAG AAACTGAAGA GTCTGGAAGC AGAAATCCTT 
CAATTGCAGG AGGAACTGGC CTCATCTGAG CGAGCGCGCC GCCACGCCGA GCAGGAGAGA 
GATGAGCTGG CTGACGAGAT CGCCAACAGC GCCTCTGGCA AGTCTGCGCT GCTGGATGAG 
AAGCGGCGTC TGGAGGCACG GATCGCGCAG CTGGAGGAGG AGCTGGAAGA GGAGCAGAGC 
AACATGGAGC TGCTCAACGA CCGCTTCCGC AAGACCACGC TGCAGGTGGA CACGCTGAAC 
GCCGAGCTCG CGGCTGAGCG CAGCGCCGCC CAGAAGAGCG ACAACGCACG CCAGCAGCTA 
GAGCGGCAGA ACAAGGAGCT GAAGGCCAAG CTGCAGGAAC TGGAGGGCGC CGTCAAGTCC 
AAGTTCAAGG CCACCATCTC TGCCCTGGAA GCCAAGATTG GGCAGCTGGA GGAGCAGCTG 
GAGCAGGAAG CCAAGGAACG AGCAGCCGCC AACAAACTAG TCCGTCGCAC GGAGAAAAAG 
CTGAAAGAGA TCTTCATGCA GGTTGAAGAT GAGCGTCGAC ATGCGGATCA GTATAAAGAA 
CAGATGGAGA AGGCCAACGC CAGGATGAAA CAGCTCAAGC GGCAGCTGGA GGAGGCAGAG 
GAGGAAGCCA CACGTGCCAA CGCATCTCGG CGTAAGCTCC AGCGGGAACT GGACGATGCC 
ACGGAGGCCA ACGAGGGCCT GAGCCGTGAG GTCAGCACGC TGAAGAACCG GCTGAGACGA 
GGTGGCCCCA TAAGCTTCTC GTCCAGCCGA TCGGGCCGGC GCCAGCTACA CATCGAGGGA 
GCCTCCCTGG AGCTGTCAGA CGATGACACG GAGAGTAAGA CCAGTGACGT CAACGAGACA 
CAGCCGCCGC AGTCGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969070.1
CDS	132..6152
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_014113595.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin, heavy chain 10, non-muscle (MYH10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113595.1

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGAATCGC CTAAACCAGT GAAACATCAG 
GGGGAACTTG AACGGCAGCT TTTGCAAGCA AATCCAATCC TGGAATCCTT TGGAAATGCG 
AAGACTGTGA AAAATGATAA TTCATCTCGC TTTGGCAAGT TTATTCGAAT CAACTTTGAT 
GTAACTGGCT ATATTGTTGG GGCCAACATT GAAACATACC TTCTGGAAAA ATCTCGTGCT 
GTTCGTCAAG CAAAAGATGA GCGTACTTTT CATATCTTTT ACCAGTTGTT ATCTGGAGCA 
GGAGAACACC TAAAGTCGGA TTTGCTCCTC GAAGGATTTA ATAACTACAG ATTCCTCTCC 
AACGGCTATA TTCCCATCCC GGGACAGCAA GACAAGGACA ACTTTCAGGA AACCATGGAA 
GCAATGCACA TCATGGGCTT CTCTCACGAG GAGATTCTGT CAATGCTTAA AGTAGTGTCA 
TCAGTGCTAC AGTTCGGAAA TATTTCTTTC AAAAAGGAGC GAAATACTGA TCAAGCATCC 
ATGCCAGAGA ACACAGTTGC ACAGAAACTC TGCCATCTTC TTGGGATGAA CGTGATGGAG 
TTCACGCGGG CCATCCTCAC CCCCCGGATC AAGGTCGGCC GAGACTATGT GCAGAAAGCC 
CAGACCAAAG AACAAGCAGA TTTTGCGGTA GAAGCATTGG CAAAAGCTAC CTATGAGCGG 
CTCTTCCGTT GGCTCGTTCA CCGTATCAAC AAAGCTCTGG ACAGGACCAA GCGTCAGGGC 
GCATCTTTTA TTGGAATCCT GGATATCGCT GGATTCGAAA TATTTGAGCT CAACTCGTTC 
GAGCAACTGT GCATCAACTA CACCAATGAG AAGCTGCAGC AGCTCTTCAA CCACACCATG 
TTCATCCTGG AGCAGGAGGA GTACCAGCGC GAGGGCATCG AGTGGAGCTT CATTGACTTT 
GGCCTCGACC TGCAGCCCTG CATCGACCTC ATCGAGAGAC CCGCTAACCC CCCTGGTGTG 
CTGGCCCTTT TGGATGAAGA GTGCTGGTTC CCGAAGGCCA CGGACAAAAC CTTTGTTGAA 
AAACTAGTTC AAGAGCAAGG CTCTCACTCC AAGTTCCAGA AACCCCGGCA ACTGAAAGAC 
AGAGCTGACT TCTGCATCAT CCATTATGCG GGCAAGGTGG ACTACAAGGC TGATGAGTGG 
CTGATGAAGA ACATGGACCC CCTGAACGAC AATGTGGCCA CCCTCCTGCA CCAGTCATCT 
GACAGATTCG TGGCAGAGCT TTGGAAGGAT GAGATTCAGA ATATTCAGAG AGCTTCTTTC 
TATGACAGTG TTTCTGGTCT TCATGAGCCA CCAGTGGACC GTATCGTGGG TCTGGATCAG 
GTCACGGGCA TGACGGAGAC AGCCTTTGGC TCTGCGTATA AAACCAAGAA GGGCATGTTT 
CGGACTGTCG GGCAGCTCTA CAAAGAATCC CTCACCAAGC TGATGGCAAC TCTCCGGAAC 
ACCAACCCTA ACTTCGTCCG CTGCATCATT CCAAATCACG AGAAGCGAGC TGGAAAACTG 
GATCCGCACC TAGTGCTTGA TCAGCTTCGC TGTAACGGCG TTCTGGAAGG AATCAGGATC 
TGTCGCCAGG GGTTCCCCAA CCGGATAGTT TTCCAGGAAT TCAGACAGAG ATACGAGATC 
CTAACTCCAA ATGCTATCCC TAAGGGTTTC ATGGACGGCA AACAGGCCTG CGAACGAATG 
ATCCGGGCTT TAGAACTGGA CCCAAACTTG TACAGAATCG GACAGAGCAA GATATTTTTC 
CGAGCTGGCG TGCTGGCACA CTTAGAGGAA GAGAGGGATC TAAAGATCAC CGACATCATT 
ATCTTCTTCC AGGCTGTCTG CAGAGGCTAC CTGGCCAGAA AGGCCTTCGC CAAGAAGCAG 
CAGCAGCTGA GCGCCCTGAA GGTCCTGCAG CGGAACTGCG CCGCATACCT GAAGCTCCGG 
CACTGGCAGT GGTGGCGTGT CTTCACGAAG GTGAAGCCGC TCCTGCAAGT GACCCGCCAG 
GAGGAAGAGC TTCAGGCCAA AGACGAGGAG CTGCTGAAGG TGAAGGAGAG GCAGACGAAG 
GTGGAGGGCG AGCTGGAGGA AATGGAGAGG AAGCACCAGC AGCTCCTGGA AGAGAAGAAC 
ATCCTAACAG AGCAGCTCCA AGCAGAGACA GAGCTCTTTG CTGAGGCAGA AGAGATGAGA 
GCCAGGCTCG CCGCCAAGAA GCAGGAGCTG GAGGAGATTC TCCATGACTT GGAATCCAGG 
GTCGAAGAGG AAGAAGAACG AAACCAAATC CTCCAAAATG AAAAGAAGAA AATGCAAGCT 
CACATTCAGG ACCTAGAGGA GCAACTGGAT GAGGAAGAAG GCGCTCGCCA GAAACTACAG 
CTGGAAAAGG TGACAGCGGA AGCAAAAATC AAGAAGATGG AGGAGGAGAT CCTGCTGCTT 
GAGGACCAGA ATTCCAAATT TATCAAAGAA AAGAAACTCA TGGAAGATCG CATTGCAGAG 
TGTTCCTCTC AGCTGGCTGA GGAGGAAGAA AAGGCCAAAA ACTTGGCCAA AATCAGGAAT 
AAGCAGGAAG TGATGATCTC AGATTTAGAA GAACGCTTGA AGAAGGAAGA GAAGACGCGT 
CAGGAGCTGG AGAAGGCCAA ACGAAAACTG GACGGTGAGA CCACTGACCT GCAGGACCAG 
ATCGCAGAGC TCCAGGCGCA GATCGACGAG CTCAAGGTCC AGCTGGCCAA GAAGGAGGAG 
GAGCTGCAAG GCGCGCTGGC CAGGGGTGAC GATGAGACGC TCCATAAGAA CAATGCCCTC 
AAGGTGGTGC GAGAGCTGCA AGCCCAAATC GCCGAGCTTC AGGAGGACTT TGAATCAGAG 
AAGGCTTCAC GGAACAAGGC GGAGAAGCAG AAGAGGGACC TGAGTGAGGA GCTGGAGGCC 
CTGAAAACAG AGCTGGAGGA CACGCTGGAC ACCACCGCGG CTCAGCAGGA ACTTCGCACG 
AAGCGTGAAC AAGAAGTGGC AGAGCTGAAG AAGGCTCTGG AGGAGGAAAC GAGGAGCCAC 
GAGGCTCAAA TCCAGGACAT GAGACAGCGA CACGCCACAG CCCTGGAGGA GCTGTCGGAG 
CAGCTGGAAC AGGCAAAGAG GTTCAAAGCG AATCTGGAGA AGAACAAGCA GGGCCTGGAG 
ACTGACAACA AGGAGCTGGC GTGTGAGGTG AAGGTGCTAC AGCAGGTCAA GGCTGAGTCT 
GAGCACAAGA GGAAGAAGCT CGACGCCCAG GTGCAGGAGC TGCACGCCAA GGTCTCAGAA 
GGAGACAGGC TCAGGGTGGA GCTGGCCGAG AAGGCAAACA AATTACAGAA TGAACTGGAC 
AATGTCTCAA GTCTTCTGGA AGAAGCAGAG AAGAAGGGCA TTAAGTTTGC TAAGGACGCA 
GCTAGTCTTG AGTCTCAACT ACAGGACACT CAGGAGCTCC TTCAGGAGGA GACCCGCCAG 
AAGCTGAACC TGAGCAGCCG CATCCGGCAG CTGGAGGAGG AGAAGAACAG TCTCCAGGAG 
CAGCAGGAGG AGGAGGAGGA GGCCAGGAAA AACCTGGAAA AGCAGGTGTT GGTGCTGCAG 
TCCCAGCTCG CCGATACCAA GAAGAAGGTA GATGATGACT TAGGAACCAT CGAGAGCTTA 
GAAGAAGCCA GAAAGAAGCT TCTGAAAGAT GTGGAGGCCC TGGGCCAGCG CCTGGAGGAG 
AAGGCTCTGG CCTATGACAA GCTGGAGAAG ACCAAGAACC GCCTGCAGCA GGAGCTCGAC 
GACCTGACAG TGGACCTAGA TCACCAGCGC CAGATCGCCT CCAACTTGGA GAAGAAGCAG 
AAGAAGTTTG ACCAGCTGCT GGCAGAGGAG AAGAACATCT CCGCCCGCTA TGCAGAAGAG 
CGAGACCGCG CAGAGGCAGA GGCCAGGGAG AAGGAAACCA AAGCCCTGTC CCTGGCCCGA 
GCCCTCGAGG AGGCCCTAGA AACCAAGGAA GAGTTTGAGA GACAGAACAA GCAGCTGCGG 
GCAGACATGG AGGACCTGAT AAGCTCCAAG GATGACGTGG GGAAGAACGT GCACGAGCTT 
GAAAAATCGA AGCGGGCCCT GGAGCAACAG GTGGAGGAGA TGAGGACACA GCTGGAAGAG 
CTGGAGGATG AGCTTCAGGC CACAGAAGAC GCCAAGCTTC GCCTGGAAGT CAACATGCAG 
GCCATGAAGG CCCAGTTTGA GAGGGACTTA CAAACCAGGG ACGAGCAGAA TGAAGAGAAG 
AAGCGGCTCC TGGTTAAGCA GGTGCGGGAG CTGGAGGCAG AGCTGGAAGA TGAGCGGAAG 
CAGCGAGCGC TGGCGGTCGC TTCTAAAAAG AAACTGGAAA TAGACCTGAA GGACCTTGAA 
GCCCAGATTG AGGCTGCGAA CAAAGCGAGG GACGAAGTGA TCAAGCAGCT TCGCAAGCTT 
CAGGCTCAGA TGAAGGATTA CCAACGTGAA TTAGAAGAAG CCCGTGCATC CCGGGATGAG 
ATTTTTGCTC AATCCAAAGA GAGTGAAAAG AAACTGAAGA GTCTGGAAGC AGAAATCCTT 
CAATTGCAGG AGGAACTGGC CTCATCTGAG CGAGCGCGCC GCCACGCCGA GCAGGAGAGA 
GATGAGCTGG CTGACGAGAT CGCCAACAGC GCCTCTGGCA AGTCTGCGCT GCTGGATGAG 
AAGCGGCGTC TGGAGGCACG GATCGCGCAG CTGGAGGAGG AGCTGGAAGA GGAGCAGAGC 
AACATGGAGC TGCTCAACGA CCGCTTCCGC AAGACCACGC TGCAGGTGGA CACGCTGAAC 
GCCGAGCTCG CGGCTGAGCG CAGCGCCGCC CAGAAGAGCG ACAACGCACG CCAGCAGCTA 
GAGCGGCAGA ACAAGGAGCT GAAGGCCAAG CTGCAGGAAC TGGAGGGCGC CGTCAAGTCC 
AAGTTCAAGG CCACCATCTC TGCCCTGGAA GCCAAGATTG GGCAGCTGGA GGAGCAGCTG 
GAGCAGGAAG CCAAGGAACG AGCAGCCGCC AACAAACTAG TCCGTCGCAC GGAGAAAAAG 
CTGAAAGAGA TCTTCATGCA GGTTGAAGAT GAGCGTCGAC ATGCGGATCA GTATAAAGAA 
CAGATGGAGA AGGCCAACGC CAGGATGAAA CAGCTCAAGC GGCAGCTGGA GGAGGCAGAG 
GAGGAAGCCA CACGTGCCAA CGCATCTCGG CGTAAGCTCC AGCGGGAACT GGACGATGCC 
ACGGAGGCCA ACGAGGGCCT GAGCCGTGAG GTCAGCACGC TGAAGAACCG GCTGAGACGA 
GGTGGCCCCA TAAGCTTCTC GTCCAGCCGA TCGGGCCGGC GCCAGCTACA CATCGAGGGA 
GCCTCCCTGG AGCTGTCAGA CGATGACACG GAGAGTAAGA CCAGTGACGT CAACGAGACA 
CAGCCGCCGC AGTCGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_014113595.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969070.1 Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X2, mRNA.

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CloneID	OCe36457
Clone ID Related Accession (Same CDS sequence)	XM_014113595.1 , XM_014113595.2
Accession Version	XM_014113595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	myosin-10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGAATCGC CTAAACCAGT GAAACATCAG 
GGGGAACTTG AACGGCAGCT TTTGCAAGCA AATCCAATCC TGGAATCCTT TGGAAATGCG 
AAGACTGTGA AAAATGATAA TTCATCTCGC TTTGGCAAGT TTATTCGAAT CAACTTTGAT 
GTAACTGGCT ATATTGTTGG GGCCAACATT GAAACATACC TTCTGGAAAA ATCTCGTGCT 
GTTCGTCAAG CAAAAGATGA GCGTACTTTT CATATCTTTT ACCAGTTGTT ATCTGGAGCA 
GGAGAACACC TAAAGTCGGA TTTGCTCCTC GAAGGATTTA ATAACTACAG ATTCCTCTCC 
AACGGCTATA TTCCCATCCC GGGACAGCAA GACAAGGACA ACTTTCAGGA AACCATGGAA 
GCAATGCACA TCATGGGCTT CTCTCACGAG GAGATTCTGT CAATGCTTAA AGTAGTGTCA 
TCAGTGCTAC AGTTCGGAAA TATTTCTTTC AAAAAGGAGC GAAATACTGA TCAAGCATCC 
ATGCCAGAGA ACACAGTTGC ACAGAAACTC TGCCATCTTC TTGGGATGAA CGTGATGGAG 
TTCACGCGGG CCATCCTCAC CCCCCGGATC AAGGTCGGCC GAGACTATGT GCAGAAAGCC 
CAGACCAAAG AACAAGCAGA TTTTGCGGTA GAAGCATTGG CAAAAGCTAC CTATGAGCGG 
CTCTTCCGTT GGCTCGTTCA CCGTATCAAC AAAGCTCTGG ACAGGACCAA GCGTCAGGGC 
GCATCTTTTA TTGGAATCCT GGATATCGCT GGATTCGAAA TATTTGAGCT CAACTCGTTC 
GAGCAACTGT GCATCAACTA CACCAATGAG AAGCTGCAGC AGCTCTTCAA CCACACCATG 
TTCATCCTGG AGCAGGAGGA GTACCAGCGC GAGGGCATCG AGTGGAGCTT CATTGACTTT 
GGCCTCGACC TGCAGCCCTG CATCGACCTC ATCGAGAGAC CCGCTAACCC CCCTGGTGTG 
CTGGCCCTTT TGGATGAAGA GTGCTGGTTC CCGAAGGCCA CGGACAAAAC CTTTGTTGAA 
AAACTAGTTC AAGAGCAAGG CTCTCACTCC AAGTTCCAGA AACCCCGGCA ACTGAAAGAC 
AGAGCTGACT TCTGCATCAT CCATTATGCG GGCAAGGTGG ACTACAAGGC TGATGAGTGG 
CTGATGAAGA ACATGGACCC CCTGAACGAC AATGTGGCCA CCCTCCTGCA CCAGTCATCT 
GACAGATTCG TGGCAGAGCT TTGGAAGGAT GAGATTCAGA ATATTCAGAG AGCTTCTTTC 
TATGACAGTG TTTCTGGTCT TCATGAGCCA CCAGTGGACC GTATCGTGGG TCTGGATCAG 
GTCACGGGCA TGACGGAGAC AGCCTTTGGC TCTGCGTATA AAACCAAGAA GGGCATGTTT 
CGGACTGTCG GGCAGCTCTA CAAAGAATCC CTCACCAAGC TGATGGCAAC TCTCCGGAAC 
ACCAACCCTA ACTTCGTCCG CTGCATCATT CCAAATCACG AGAAGCGAGC TGGAAAACTG 
GATCCGCACC TAGTGCTTGA TCAGCTTCGC TGTAACGGCG TTCTGGAAGG AATCAGGATC 
TGTCGCCAGG GGTTCCCCAA CCGGATAGTT TTCCAGGAAT TCAGACAGAG ATACGAGATC 
CTAACTCCAA ATGCTATCCC TAAGGGTTTC ATGGACGGCA AACAGGCCTG CGAACGAATG 
ATCCGGGCTT TAGAACTGGA CCCAAACTTG TACAGAATCG GACAGAGCAA GATATTTTTC 
CGAGCTGGCG TGCTGGCACA CTTAGAGGAA GAGAGGGATC TAAAGATCAC CGACATCATT 
ATCTTCTTCC AGGCTGTCTG CAGAGGCTAC CTGGCCAGAA AGGCCTTCGC CAAGAAGCAG 
CAGCAGCTGA GCGCCCTGAA GGTCCTGCAG CGGAACTGCG CCGCATACCT GAAGCTCCGG 
CACTGGCAGT GGTGGCGTGT CTTCACGAAG GTGAAGCCGC TCCTGCAAGT GACCCGCCAG 
GAGGAAGAGC TTCAGGCCAA AGACGAGGAG CTGCTGAAGG TGAAGGAGAG GCAGACGAAG 
GTGGAGGGCG AGCTGGAGGA AATGGAGAGG AAGCACCAGC AGCTCCTGGA AGAGAAGAAC 
ATCCTAACAG AGCAGCTCCA AGCAGAGACA GAGCTCTTTG CTGAGGCAGA AGAGATGAGA 
GCCAGGCTCG CCGCCAAGAA GCAGGAGCTG GAGGAGATTC TCCATGACTT GGAATCCAGG 
GTCGAAGAGG AAGAAGAACG AAACCAAATC CTCCAAAATG AAAAGAAGAA AATGCAAGCT 
CACATTCAGG ACCTAGAGGA GCAACTGGAT GAGGAAGAAG GCGCTCGCCA GAAACTACAG 
CTGGAAAAGG TGACAGCGGA AGCAAAAATC AAGAAGATGG AGGAGGAGAT CCTGCTGCTT 
GAGGACCAGA ATTCCAAATT TATCAAAGAA AAGAAACTCA TGGAAGATCG CATTGCAGAG 
TGTTCCTCTC AGCTGGCTGA GGAGGAAGAA AAGGCCAAAA ACTTGGCCAA AATCAGGAAT 
AAGCAGGAAG TGATGATCTC AGATTTAGAA GAACGCTTGA AGAAGGAAGA GAAGACGCGT 
CAGGAGCTGG AGAAGGCCAA ACGAAAACTG GACGGTGAGA CCACTGACCT GCAGGACCAG 
ATCGCAGAGC TCCAGGCGCA GATCGACGAG CTCAAGGTCC AGCTGGCCAA GAAGGAGGAG 
GAGCTGCAAG GCGCGCTGGC CAGGGGTGAC GATGAGACGC TCCATAAGAA CAATGCCCTC 
AAGGTGGTGC GAGAGCTGCA AGCCCAAATC GCCGAGCTTC AGGAGGACTT TGAATCAGAG 
AAGGCTTCAC GGAACAAGGC GGAGAAGCAG AAGAGGGACC TGAGTGAGGA GCTGGAGGCC 
CTGAAAACAG AGCTGGAGGA CACGCTGGAC ACCACCGCGG CTCAGCAGGA ACTTCGCACG 
AAGCGTGAAC AAGAAGTGGC AGAGCTGAAG AAGGCTCTGG AGGAGGAAAC GAGGAGCCAC 
GAGGCTCAAA TCCAGGACAT GAGACAGCGA CACGCCACAG CCCTGGAGGA GCTGTCGGAG 
CAGCTGGAAC AGGCAAAGAG GTTCAAAGCG AATCTGGAGA AGAACAAGCA GGGCCTGGAG 
ACTGACAACA AGGAGCTGGC GTGTGAGGTG AAGGTGCTAC AGCAGGTCAA GGCTGAGTCT 
GAGCACAAGA GGAAGAAGCT CGACGCCCAG GTGCAGGAGC TGCACGCCAA GGTCTCAGAA 
GGAGACAGGC TCAGGGTGGA GCTGGCCGAG AAGGCAAACA AATTACAGAA TGAACTGGAC 
AATGTCTCAA GTCTTCTGGA AGAAGCAGAG AAGAAGGGCA TTAAGTTTGC TAAGGACGCA 
GCTAGTCTTG AGTCTCAACT ACAGGACACT CAGGAGCTCC TTCAGGAGGA GACCCGCCAG 
AAGCTGAACC TGAGCAGCCG CATCCGGCAG CTGGAGGAGG AGAAGAACAG TCTCCAGGAG 
CAGCAGGAGG AGGAGGAGGA GGCCAGGAAA AACCTGGAAA AGCAGGTGTT GGTGCTGCAG 
TCCCAGCTCG CCGATACCAA GAAGAAGGTA GATGATGACT TAGGAACCAT CGAGAGCTTA 
GAAGAAGCCA GAAAGAAGCT TCTGAAAGAT GTGGAGGCCC TGGGCCAGCG CCTGGAGGAG 
AAGGCTCTGG CCTATGACAA GCTGGAGAAG ACCAAGAACC GCCTGCAGCA GGAGCTCGAC 
GACCTGACAG TGGACCTAGA TCACCAGCGC CAGATCGCCT CCAACTTGGA GAAGAAGCAG 
AAGAAGTTTG ACCAGCTGCT GGCAGAGGAG AAGAACATCT CCGCCCGCTA TGCAGAAGAG 
CGAGACCGCG CAGAGGCAGA GGCCAGGGAG AAGGAAACCA AAGCCCTGTC CCTGGCCCGA 
GCCCTCGAGG AGGCCCTAGA AACCAAGGAA GAGTTTGAGA GACAGAACAA GCAGCTGCGG 
GCAGACATGG AGGACCTGAT AAGCTCCAAG GATGACGTGG GGAAGAACGT GCACGAGCTT 
GAAAAATCGA AGCGGGCCCT GGAGCAACAG GTGGAGGAGA TGAGGACACA GCTGGAAGAG 
CTGGAGGATG AGCTTCAGGC CACAGAAGAC GCCAAGCTTC GCCTGGAAGT CAACATGCAG 
GCCATGAAGG CCCAGTTTGA GAGGGACTTA CAAACCAGGG ACGAGCAGAA TGAAGAGAAG 
AAGCGGCTCC TGGTTAAGCA GGTGCGGGAG CTGGAGGCAG AGCTGGAAGA TGAGCGGAAG 
CAGCGAGCGC TGGCGGTCGC TTCTAAAAAG AAACTGGAAA TAGACCTGAA GGACCTTGAA 
GCCCAGATTG AGGCTGCGAA CAAAGCGAGG GACGAAGTGA TCAAGCAGCT TCGCAAGCTT 
CAGGCTCAGA TGAAGGATTA CCAACGTGAA TTAGAAGAAG CCCGTGCATC CCGGGATGAG 
ATTTTTGCTC AATCCAAAGA GAGTGAAAAG AAACTGAAGA GTCTGGAAGC AGAAATCCTT 
CAATTGCAGG AGGAACTGGC CTCATCTGAG CGAGCGCGCC GCCACGCCGA GCAGGAGAGA 
GATGAGCTGG CTGACGAGAT CGCCAACAGC GCCTCTGGCA AGTCTGCGCT GCTGGATGAG 
AAGCGGCGTC TGGAGGCACG GATCGCGCAG CTGGAGGAGG AGCTGGAAGA GGAGCAGAGC 
AACATGGAGC TGCTCAACGA CCGCTTCCGC AAGACCACGC TGCAGGTGGA CACGCTGAAC 
GCCGAGCTCG CGGCTGAGCG CAGCGCCGCC CAGAAGAGCG ACAACGCACG CCAGCAGCTA 
GAGCGGCAGA ACAAGGAGCT GAAGGCCAAG CTGCAGGAAC TGGAGGGCGC CGTCAAGTCC 
AAGTTCAAGG CCACCATCTC TGCCCTGGAA GCCAAGATTG GGCAGCTGGA GGAGCAGCTG 
GAGCAGGAAG CCAAGGAACG AGCAGCCGCC AACAAACTAG TCCGTCGCAC GGAGAAAAAG 
CTGAAAGAGA TCTTCATGCA GGTTGAAGAT GAGCGTCGAC ATGCGGATCA GTATAAAGAA 
CAGATGGAGA AGGCCAACGC CAGGATGAAA CAGCTCAAGC GGCAGCTGGA GGAGGCAGAG 
GAGGAAGCCA CACGTGCCAA CGCATCTCGG CGTAAGCTCC AGCGGGAACT GGACGATGCC 
ACGGAGGCCA ACGAGGGCCT GAGCCGTGAG GTCAGCACGC TGAAGAACCG GCTGAGACGA 
GGTGGCCCCA TAAGCTTCTC GTCCAGCCGA TCGGGCCGGC GCCAGCTACA CATCGAGGGA 
GCCTCCCTGG AGCTGTCAGA CGATGACACG GAGAGTAAGA CCAGTGACGT CAACGAGACA 
CAGCCGCCGC AGTCGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969070.1
CDS	192..6212
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDRADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKERQTKVEGELEEMERKHQQLLEEKNILTEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKVQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETRSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSSLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLVLQSQLADTKKKVDDDLGTIESLEEARKKLLKDVEALGQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQIASNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALETKEEFERQNKQLRADMEDLISSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLVKQVRELEAELEDERKQRALAVASKKKLEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDVNETQPPQSE

Target ORF information:

RefSeq Version	XM_014113595.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin heavy chain 10 (MYH10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113595.2

ORF Insert Sequence:

ATGGCCCAGA GAACTGGACT TGAAGACCCA GAGAGGTACC TCTTTGTGGA CAGGGCTGTC 
ATCTATAACC CTGCCACCCA GGCCGACTGG ACGGCTAAAA AGCTAGTGTG GATTCCATCA 
GAGCGCCATG GTTTCGAGGC AGCAAGTATC AAAGAGGAGC GGGGAGATGA GGTTATGGTG 
GAGTTGGCAG AGAACGGCAA GAAAGCCATG GTCAACAAAG ACGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCGGAATTGA CGTGCTTGAA CGAAGCTTCT 
GTTCTACATA ACCTGAAGGA CCGCTACTAT TCGGGATTGA TCTATACTTA CTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAT CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAACG CCACGAGATG CCTCCACACA TCTACGCTAT ATCTGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCCA TTCTTTGCAC GGGCGAATCA 
GGTGCTGGGA AGACAGAAAA TACCAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCGCATAAAG GACGGAAGGA CCATAATATT CCTGAATCGC CTAAACCAGT GAAACATCAG 
GGGGAACTTG AACGGCAGCT TTTGCAAGCA AATCCAATCC TGGAATCCTT TGGAAATGCG 
AAGACTGTGA AAAATGATAA TTCATCTCGC TTTGGCAAGT TTATTCGAAT CAACTTTGAT 
GTAACTGGCT ATATTGTTGG GGCCAACATT GAAACATACC TTCTGGAAAA ATCTCGTGCT 
GTTCGTCAAG CAAAAGATGA GCGTACTTTT CATATCTTTT ACCAGTTGTT ATCTGGAGCA 
GGAGAACACC TAAAGTCGGA TTTGCTCCTC GAAGGATTTA ATAACTACAG ATTCCTCTCC 
AACGGCTATA TTCCCATCCC GGGACAGCAA GACAAGGACA ACTTTCAGGA AACCATGGAA 
GCAATGCACA TCATGGGCTT CTCTCACGAG GAGATTCTGT CAATGCTTAA AGTAGTGTCA 
TCAGTGCTAC AGTTCGGAAA TATTTCTTTC AAAAAGGAGC GAAATACTGA TCAAGCATCC 
ATGCCAGAGA ACACAGTTGC ACAGAAACTC TGCCATCTTC TTGGGATGAA CGTGATGGAG 
TTCACGCGGG CCATCCTCAC CCCCCGGATC AAGGTCGGCC GAGACTATGT GCAGAAAGCC 
CAGACCAAAG AACAAGCAGA TTTTGCGGTA GAAGCATTGG CAAAAGCTAC CTATGAGCGG 
CTCTTCCGTT GGCTCGTTCA CCGTATCAAC AAAGCTCTGG ACAGGACCAA GCGTCAGGGC 
GCATCTTTTA TTGGAATCCT GGATATCGCT GGATTCGAAA TATTTGAGCT CAACTCGTTC 
GAGCAACTGT GCATCAACTA CACCAATGAG AAGCTGCAGC AGCTCTTCAA CCACACCATG 
TTCATCCTGG AGCAGGAGGA GTACCAGCGC GAGGGCATCG AGTGGAGCTT CATTGACTTT 
GGCCTCGACC TGCAGCCCTG CATCGACCTC ATCGAGAGAC CCGCTAACCC CCCTGGTGTG 
CTGGCCCTTT TGGATGAAGA GTGCTGGTTC CCGAAGGCCA CGGACAAAAC CTTTGTTGAA 
AAACTAGTTC AAGAGCAAGG CTCTCACTCC AAGTTCCAGA AACCCCGGCA ACTGAAAGAC 
AGAGCTGACT TCTGCATCAT CCATTATGCG GGCAAGGTGG ACTACAAGGC TGATGAGTGG 
CTGATGAAGA ACATGGACCC CCTGAACGAC AATGTGGCCA CCCTCCTGCA CCAGTCATCT 
GACAGATTCG TGGCAGAGCT TTGGAAGGAT GAGATTCAGA ATATTCAGAG AGCTTCTTTC 
TATGACAGTG TTTCTGGTCT TCATGAGCCA CCAGTGGACC GTATCGTGGG TCTGGATCAG 
GTCACGGGCA TGACGGAGAC AGCCTTTGGC TCTGCGTATA AAACCAAGAA GGGCATGTTT 
CGGACTGTCG GGCAGCTCTA CAAAGAATCC CTCACCAAGC TGATGGCAAC TCTCCGGAAC 
ACCAACCCTA ACTTCGTCCG CTGCATCATT CCAAATCACG AGAAGCGAGC TGGAAAACTG 
GATCCGCACC TAGTGCTTGA TCAGCTTCGC TGTAACGGCG TTCTGGAAGG AATCAGGATC 
TGTCGCCAGG GGTTCCCCAA CCGGATAGTT TTCCAGGAAT TCAGACAGAG ATACGAGATC 
CTAACTCCAA ATGCTATCCC TAAGGGTTTC ATGGACGGCA AACAGGCCTG CGAACGAATG 
ATCCGGGCTT TAGAACTGGA CCCAAACTTG TACAGAATCG GACAGAGCAA GATATTTTTC 
CGAGCTGGCG TGCTGGCACA CTTAGAGGAA GAGAGGGATC TAAAGATCAC CGACATCATT 
ATCTTCTTCC AGGCTGTCTG CAGAGGCTAC CTGGCCAGAA AGGCCTTCGC CAAGAAGCAG 
CAGCAGCTGA GCGCCCTGAA GGTCCTGCAG CGGAACTGCG CCGCATACCT GAAGCTCCGG 
CACTGGCAGT GGTGGCGTGT CTTCACGAAG GTGAAGCCGC TCCTGCAAGT GACCCGCCAG 
GAGGAAGAGC TTCAGGCCAA AGACGAGGAG CTGCTGAAGG TGAAGGAGAG GCAGACGAAG 
GTGGAGGGCG AGCTGGAGGA AATGGAGAGG AAGCACCAGC AGCTCCTGGA AGAGAAGAAC 
ATCCTAACAG AGCAGCTCCA AGCAGAGACA GAGCTCTTTG CTGAGGCAGA AGAGATGAGA 
GCCAGGCTCG CCGCCAAGAA GCAGGAGCTG GAGGAGATTC TCCATGACTT GGAATCCAGG 
GTCGAAGAGG AAGAAGAACG AAACCAAATC CTCCAAAATG AAAAGAAGAA AATGCAAGCT 
CACATTCAGG ACCTAGAGGA GCAACTGGAT GAGGAAGAAG GCGCTCGCCA GAAACTACAG 
CTGGAAAAGG TGACAGCGGA AGCAAAAATC AAGAAGATGG AGGAGGAGAT CCTGCTGCTT 
GAGGACCAGA ATTCCAAATT TATCAAAGAA AAGAAACTCA TGGAAGATCG CATTGCAGAG 
TGTTCCTCTC AGCTGGCTGA GGAGGAAGAA AAGGCCAAAA ACTTGGCCAA AATCAGGAAT 
AAGCAGGAAG TGATGATCTC AGATTTAGAA GAACGCTTGA AGAAGGAAGA GAAGACGCGT 
CAGGAGCTGG AGAAGGCCAA ACGAAAACTG GACGGTGAGA CCACTGACCT GCAGGACCAG 
ATCGCAGAGC TCCAGGCGCA GATCGACGAG CTCAAGGTCC AGCTGGCCAA GAAGGAGGAG 
GAGCTGCAAG GCGCGCTGGC CAGGGGTGAC GATGAGACGC TCCATAAGAA CAATGCCCTC 
AAGGTGGTGC GAGAGCTGCA AGCCCAAATC GCCGAGCTTC AGGAGGACTT TGAATCAGAG 
AAGGCTTCAC GGAACAAGGC GGAGAAGCAG AAGAGGGACC TGAGTGAGGA GCTGGAGGCC 
CTGAAAACAG AGCTGGAGGA CACGCTGGAC ACCACCGCGG CTCAGCAGGA ACTTCGCACG 
AAGCGTGAAC AAGAAGTGGC AGAGCTGAAG AAGGCTCTGG AGGAGGAAAC GAGGAGCCAC 
GAGGCTCAAA TCCAGGACAT GAGACAGCGA CACGCCACAG CCCTGGAGGA GCTGTCGGAG 
CAGCTGGAAC AGGCAAAGAG GTTCAAAGCG AATCTGGAGA AGAACAAGCA GGGCCTGGAG 
ACTGACAACA AGGAGCTGGC GTGTGAGGTG AAGGTGCTAC AGCAGGTCAA GGCTGAGTCT 
GAGCACAAGA GGAAGAAGCT CGACGCCCAG GTGCAGGAGC TGCACGCCAA GGTCTCAGAA 
GGAGACAGGC TCAGGGTGGA GCTGGCCGAG AAGGCAAACA AATTACAGAA TGAACTGGAC 
AATGTCTCAA GTCTTCTGGA AGAAGCAGAG AAGAAGGGCA TTAAGTTTGC TAAGGACGCA 
GCTAGTCTTG AGTCTCAACT ACAGGACACT CAGGAGCTCC TTCAGGAGGA GACCCGCCAG 
AAGCTGAACC TGAGCAGCCG CATCCGGCAG CTGGAGGAGG AGAAGAACAG TCTCCAGGAG 
CAGCAGGAGG AGGAGGAGGA GGCCAGGAAA AACCTGGAAA AGCAGGTGTT GGTGCTGCAG 
TCCCAGCTCG CCGATACCAA GAAGAAGGTA GATGATGACT TAGGAACCAT CGAGAGCTTA 
GAAGAAGCCA GAAAGAAGCT TCTGAAAGAT GTGGAGGCCC TGGGCCAGCG CCTGGAGGAG 
AAGGCTCTGG CCTATGACAA GCTGGAGAAG ACCAAGAACC GCCTGCAGCA GGAGCTCGAC 
GACCTGACAG TGGACCTAGA TCACCAGCGC CAGATCGCCT CCAACTTGGA GAAGAAGCAG 
AAGAAGTTTG ACCAGCTGCT GGCAGAGGAG AAGAACATCT CCGCCCGCTA TGCAGAAGAG 
CGAGACCGCG CAGAGGCAGA GGCCAGGGAG AAGGAAACCA AAGCCCTGTC CCTGGCCCGA 
GCCCTCGAGG AGGCCCTAGA AACCAAGGAA GAGTTTGAGA GACAGAACAA GCAGCTGCGG 
GCAGACATGG AGGACCTGAT AAGCTCCAAG GATGACGTGG GGAAGAACGT GCACGAGCTT 
GAAAAATCGA AGCGGGCCCT GGAGCAACAG GTGGAGGAGA TGAGGACACA GCTGGAAGAG 
CTGGAGGATG AGCTTCAGGC CACAGAAGAC GCCAAGCTTC GCCTGGAAGT CAACATGCAG 
GCCATGAAGG CCCAGTTTGA GAGGGACTTA CAAACCAGGG ACGAGCAGAA TGAAGAGAAG 
AAGCGGCTCC TGGTTAAGCA GGTGCGGGAG CTGGAGGCAG AGCTGGAAGA TGAGCGGAAG 
CAGCGAGCGC TGGCGGTCGC TTCTAAAAAG AAACTGGAAA TAGACCTGAA GGACCTTGAA 
GCCCAGATTG AGGCTGCGAA CAAAGCGAGG GACGAAGTGA TCAAGCAGCT TCGCAAGCTT 
CAGGCTCAGA TGAAGGATTA CCAACGTGAA TTAGAAGAAG CCCGTGCATC CCGGGATGAG 
ATTTTTGCTC AATCCAAAGA GAGTGAAAAG AAACTGAAGA GTCTGGAAGC AGAAATCCTT 
CAATTGCAGG AGGAACTGGC CTCATCTGAG CGAGCGCGCC GCCACGCCGA GCAGGAGAGA 
GATGAGCTGG CTGACGAGAT CGCCAACAGC GCCTCTGGCA AGTCTGCGCT GCTGGATGAG 
AAGCGGCGTC TGGAGGCACG GATCGCGCAG CTGGAGGAGG AGCTGGAAGA GGAGCAGAGC 
AACATGGAGC TGCTCAACGA CCGCTTCCGC AAGACCACGC TGCAGGTGGA CACGCTGAAC 
GCCGAGCTCG CGGCTGAGCG CAGCGCCGCC CAGAAGAGCG ACAACGCACG CCAGCAGCTA 
GAGCGGCAGA ACAAGGAGCT GAAGGCCAAG CTGCAGGAAC TGGAGGGCGC CGTCAAGTCC 
AAGTTCAAGG CCACCATCTC TGCCCTGGAA GCCAAGATTG GGCAGCTGGA GGAGCAGCTG 
GAGCAGGAAG CCAAGGAACG AGCAGCCGCC AACAAACTAG TCCGTCGCAC GGAGAAAAAG 
CTGAAAGAGA TCTTCATGCA GGTTGAAGAT GAGCGTCGAC ATGCGGATCA GTATAAAGAA 
CAGATGGAGA AGGCCAACGC CAGGATGAAA CAGCTCAAGC GGCAGCTGGA GGAGGCAGAG 
GAGGAAGCCA CACGTGCCAA CGCATCTCGG CGTAAGCTCC AGCGGGAACT GGACGATGCC 
ACGGAGGCCA ACGAGGGCCT GAGCCGTGAG GTCAGCACGC TGAAGAACCG GCTGAGACGA 
GGTGGCCCCA TAAGCTTCTC GTCCAGCCGA TCGGGCCGGC GCCAGCTACA CATCGAGGGA 
GCCTCCCTGG AGCTGTCAGA CGATGACACG GAGAGTAAGA CCAGTGACGT CAACGAGACA 
CAGCCGCCGC AGTCGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MYH10
Entrez Gene ID	479497
Full Name	myosin heavy chain 10
General protein information	Preferred Names myosin heavy chain 10 Names myosin-10
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

Macaca mulatta (Rhesus monkey)	MYH10	XP_001118181.2
Rattus norvegicus (Norway rat)	Myh10	NP_113708.1
Gallus gallus (chicken)	MYH10	NP_990805.1
Pan troglodytes (chimpanzee)	MYH10	XP_001166541.2
Homo sapiens (human)	MYH10	NP_001242941.1
Canis lupus familiaris (dog)	MYH10	XP_005620092.1
Caenorhabditis elegans (roundworm)	nmy-1	NP_508504.2
Xenopus tropicalis (tropical clawed frog)	LOC100493774	XP_004916274.1
Mus musculus (house mouse)	Myh10	NP_780469.1
Drosophila melanogaster (fruit fly)	zip	NP_523860.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007523	XP_308355.3
Bos taurus (cattle)	MYH10	NP_777259.1
Danio rerio (zebrafish)	myh10	XP_005165776.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MYH10 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

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