BRAF cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAF
Entrez Gene ID	475526
Full Name	B-Raf proto-oncogene, serine/threonine kinase
General protein information	Preferred Names B-Raf proto-oncogene, serine/threonine kinase Names serine/threonine-protein kinase B-raf B-raf protein v-raf murine sarcoma viral oncogene homolog B1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005629550.2	XP_005629607.1	serine/threonine-protein kinase B-raf isoform X2
XM_005629551.3	XP_005629608.1	serine/threonine-protein kinase B-raf isoform X2
XM_022403736.1	XP_022259444.1	serine/threonine-protein kinase B-raf isoform X4
XM_022403735.1	XP_022259443.1	serine/threonine-protein kinase B-raf isoform X3
XM_014119889.1	XP_013975364.1	serine/threonine-protein kinase B-raf isoform X1
XM_005629551.2	XP_005629608.1	serine/threonine-protein kinase B-raf isoform X3
XM_005629550.3	XP_005629607.1	serine/threonine-protein kinase B-raf isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Braf	NP_647455.3
Rattus norvegicus (Norway rat)	Braf	XP_006236418.1
Gallus gallus (chicken)	BRAF	NP_990633.1
Caenorhabditis elegans (roundworm)	lin-45	NP_741430.3
Pan troglodytes (chimpanzee)	BRAF	XP_003951208.1
Macaca mulatta (Rhesus monkey)	LOC693554	XP_002803541.1
Bos taurus (cattle)	BRAF	XP_005205968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004699	XP_318144.4
Canis lupus familiaris (dog)	BRAF	XP_005629607.1
Drosophila melanogaster (fruit fly)	phl	NP_525047.1
Xenopus tropicalis (tropical clawed frog)	braf	XP_004912520.1
Danio rerio (zebrafish)	braf	NP_991307.2
Homo sapiens (human)	BRAF	NP_004324.2

Gene Ontology

The following BRAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe17979	XM_005629550.3 Latest version!	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe17980	XM_005629551.3 Latest version!	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe293847	XM_022403735.1 Latest version!	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe293848	XM_022403736.1 Latest version!	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe40466	XM_014119889.1 Latest version!	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OCe17980	XM_005629551.2	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe17979	XM_005629550.2	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAF ( XM_005629550.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629607.1 Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe17979
Clone ID Related Accession (Same CDS sequence)	XM_005629550.2 , XM_005629550.3
Accession Version	XM_005629550.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase B-raf isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629550.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGATT TTCATGGAAT AAGTTTGACC TGTGCTGCAG TGGCCTCCAG CAAGGTACCC 
GCAAGATGTG GAGTTACAGT CCGGGACAGT CTAAAGAAAG CTCTGATGAT GAGAGGTCTA 
ATCCCAGAGT GCTGTGCTGT TTACAGAATT CAGGATGGAG AGAAGAAACC GATTGGCTGG 
GACACTGATA TTTCCTGGCT CACTGGAGAG GAATTGCATG TAGAAGTGTT GGAAAATGTT 
CCGCTTACCA CACACAACTT TGTACGGAAA ACTTTTTTCA CCTTAGCATT TTGTGACTTT 
TGTCGAAAGC TGCTTTTCCA GGGTTTTCGC TGTCAAACAT GTGGTTATAA ATTTCACCAG 
CGTTGTAGTA CAGAGGTTCC ACTGATGTGT GTTAATTATG ACCAACTTGA TTTGCTGTTT 
GTCTCCAAGT TCTTTGAACA CCACCCAATA CCACAGGAGG AGGCCTCCAT AGCAGAGACT 
GCCCTTACGT CTGGATCATC CCCTTCTGCT CCCCCCTCCG ATTCTCCTGG GCCCCCAATT 
CTGACCAGTC CGTCTCCTTC AAAATCCATT CCAATTCCAC AGCCTTTCCG ACCAGCAGAT 
GAAGATCATC GAAATCAGTT TGGACAACGA GACCGGTCCT CATCAGCTCC AAATGTGCAT 
ATAAACACAA TAGAACCCGT CAACATTGAT GACTTGATTA GAGACCAAGG GTTTCGTAGT 
GATGGAGGAT CAACCACAGG TTTGTCTGCC ACCCCCCCTG CCTCATTGCC TGGCTCACTC 
ACTAATGTAA AAGCATTACA GAAATCTCCA GGACCTCAGC GGGAAAGAAA ATCATCTTCA 
TCCTCAGAAG ATAGGAATCG AATGAAAACA CTTGGTAGAC GGGATTCAAG TGATGATTGG 
GAGATACCTG ATGGGCAGAT CACAGTGGGA CAGAGAATTG GATCCGGGTC ATTTGGGACA 
GTCTACAAGG GAAAGTGGCA TGGTGACGTG GCAGTGAAAA TGTTGAATGT GACAGCACCC 
ACACCTCAGC AGTTACAGGC CTTCAAAAAT GAAGTAGGAG TACTCAGGAA AACTCGACAT 
GTGAATATCC TACTCTTTAT GGGCTATTCA ACAAAGCCCC AACTGGCTAT TGTTACCCAG 
TGGTGTGAGG GCTCCAGCTT ATATCACCAT CTCCACATCA TTGAGACCAA ATTTGAGATG 
ATAAAGCTTA TAGATATTGC ACGGCAGACT GCACAGGGCA TGGATTACTT ACACGCCAAG 
TCAATCATCC ACAGAGACCT CAAGAGTAAT AATATTTTTC TTCATGAAGA CCTCACAGTA 
AAAATAGGTG ATTTTGGTCT AGCCACAGTG AAATCTCGAT GGAGTGGGTC CCATCAGTTT 
GAACAGTTGT CTGGATCCAT TTTGTGGATG GCACCAGAAG TGATCCGAAT GCAAGACAAA 
AACCCATATA GCTTCCAGTC AGATGTATAC GCATTTGGGA TTGTTCTATA TGAATTGATG 
ACAGGGCAGT TACCTTATTC AAACATCAAC AACAGGGACC AGATAATTTT TATGGTGGGA 
CGAGGATATC TTTCTCCAGA TCTCAGTAAG GTACGGAGTA ACTGTCCAAA AGCCATGAAG 
AGATTGATGG CAGAGTGCCT AAAAAAGAAA AGAGATGAGA GGCCACTCTT TCCCCAAATT 
CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA TTGCCAAAAA TTCACCGCAG TGCATCAGAA 
CCCTCCTTGA ATCGGGCTGG CTTCCAAACA GAGGATTTTA GTCTCTATGC TTGCGCTTCT 
CCAAAAACAC CCATCCAGGC AGGGGGATAC GGAGAATTTG CAGCCTTCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629607.1
CDS	16..1869
Translation	MLDFHGISLTCAAVASSKVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASIAETALTSGSSPSAPPSDSPGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRSDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Target ORF information:

RefSeq Version	XM_005629550.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629550.2

ORF Insert Sequence:

ATGCTGGATT TTCATGGAAT AAGTTTGACC TGTGCTGCAG TGGCCTCCAG CAAGGTACCC 
GCAAGATGTG GAGTTACAGT CCGGGACAGT CTAAAGAAAG CTCTGATGAT GAGAGGTCTA 
ATCCCAGAGT GCTGTGCTGT TTACAGAATT CAGGATGGAG AGAAGAAACC GATTGGCTGG 
GACACTGATA TTTCCTGGCT CACTGGAGAG GAATTGCATG TAGAAGTGTT GGAAAATGTT 
CCGCTTACCA CACACAACTT TGTACGGAAA ACTTTTTTCA CCTTAGCATT TTGTGACTTT 
TGTCGAAAGC TGCTTTTCCA GGGTTTTCGC TGTCAAACAT GTGGTTATAA ATTTCACCAG 
CGTTGTAGTA CAGAGGTTCC ACTGATGTGT GTTAATTATG ACCAACTTGA TTTGCTGTTT 
GTCTCCAAGT TCTTTGAACA CCACCCAATA CCACAGGAGG AGGCCTCCAT AGCAGAGACT 
GCCCTTACGT CTGGATCATC CCCTTCTGCT CCCCCCTCCG ATTCTCCTGG GCCCCCAATT 
CTGACCAGTC CGTCTCCTTC AAAATCCATT CCAATTCCAC AGCCTTTCCG ACCAGCAGAT 
GAAGATCATC GAAATCAGTT TGGACAACGA GACCGGTCCT CATCAGCTCC AAATGTGCAT 
ATAAACACAA TAGAACCCGT CAACATTGAT GACTTGATTA GAGACCAAGG GTTTCGTAGT 
GATGGAGGAT CAACCACAGG TTTGTCTGCC ACCCCCCCTG CCTCATTGCC TGGCTCACTC 
ACTAATGTAA AAGCATTACA GAAATCTCCA GGACCTCAGC GGGAAAGAAA ATCATCTTCA 
TCCTCAGAAG ATAGGAATCG AATGAAAACA CTTGGTAGAC GGGATTCAAG TGATGATTGG 
GAGATACCTG ATGGGCAGAT CACAGTGGGA CAGAGAATTG GATCCGGGTC ATTTGGGACA 
GTCTACAAGG GAAAGTGGCA TGGTGACGTG GCAGTGAAAA TGTTGAATGT GACAGCACCC 
ACACCTCAGC AGTTACAGGC CTTCAAAAAT GAAGTAGGAG TACTCAGGAA AACTCGACAT 
GTGAATATCC TACTCTTTAT GGGCTATTCA ACAAAGCCCC AACTGGCTAT TGTTACCCAG 
TGGTGTGAGG GCTCCAGCTT ATATCACCAT CTCCACATCA TTGAGACCAA ATTTGAGATG 
ATAAAGCTTA TAGATATTGC ACGGCAGACT GCACAGGGCA TGGATTACTT ACACGCCAAG 
TCAATCATCC ACAGAGACCT CAAGAGTAAT AATATTTTTC TTCATGAAGA CCTCACAGTA 
AAAATAGGTG ATTTTGGTCT AGCCACAGTG AAATCTCGAT GGAGTGGGTC CCATCAGTTT 
GAACAGTTGT CTGGATCCAT TTTGTGGATG GCACCAGAAG TGATCCGAAT GCAAGACAAA 
AACCCATATA GCTTCCAGTC AGATGTATAC GCATTTGGGA TTGTTCTATA TGAATTGATG 
ACAGGGCAGT TACCTTATTC AAACATCAAC AACAGGGACC AGATAATTTT TATGGTGGGA 
CGAGGATATC TTTCTCCAGA TCTCAGTAAG GTACGGAGTA ACTGTCCAAA AGCCATGAAG 
AGATTGATGG CAGAGTGCCT AAAAAAGAAA AGAGATGAGA GGCCACTCTT TCCCCAAATT 
CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA TTGCCAAAAA TTCACCGCAG TGCATCAGAA 
CCCTCCTTGA ATCGGGCTGG CTTCCAAACA GAGGATTTTA GTCTCTATGC TTGCGCTTCT 
CCAAAAACAC CCATCCAGGC AGGGGGATAC GGAGAATTTG CAGCCTTCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_005629550.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629607.1 Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe17979
Clone ID Related Accession (Same CDS sequence)	XM_005629550.2 , XM_005629550.3
Accession Version	XM_005629550.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase B-raf isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629550.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGATT TTCATGGAAT AAGTTTGACC TGTGCTGCAG TGGCCTCCAG CAAGGTACCC 
GCAAGATGTG GAGTTACAGT CCGGGACAGT CTAAAGAAAG CTCTGATGAT GAGAGGTCTA 
ATCCCAGAGT GCTGTGCTGT TTACAGAATT CAGGATGGAG AGAAGAAACC GATTGGCTGG 
GACACTGATA TTTCCTGGCT CACTGGAGAG GAATTGCATG TAGAAGTGTT GGAAAATGTT 
CCGCTTACCA CACACAACTT TGTACGGAAA ACTTTTTTCA CCTTAGCATT TTGTGACTTT 
TGTCGAAAGC TGCTTTTCCA GGGTTTTCGC TGTCAAACAT GTGGTTATAA ATTTCACCAG 
CGTTGTAGTA CAGAGGTTCC ACTGATGTGT GTTAATTATG ACCAACTTGA TTTGCTGTTT 
GTCTCCAAGT TCTTTGAACA CCACCCAATA CCACAGGAGG AGGCCTCCAT AGCAGAGACT 
GCCCTTACGT CTGGATCATC CCCTTCTGCT CCCCCCTCCG ATTCTCCTGG GCCCCCAATT 
CTGACCAGTC CGTCTCCTTC AAAATCCATT CCAATTCCAC AGCCTTTCCG ACCAGCAGAT 
GAAGATCATC GAAATCAGTT TGGACAACGA GACCGGTCCT CATCAGCTCC AAATGTGCAT 
ATAAACACAA TAGAACCCGT CAACATTGAT GACTTGATTA GAGACCAAGG GTTTCGTAGT 
GATGGAGGAT CAACCACAGG TTTGTCTGCC ACCCCCCCTG CCTCATTGCC TGGCTCACTC 
ACTAATGTAA AAGCATTACA GAAATCTCCA GGACCTCAGC GGGAAAGAAA ATCATCTTCA 
TCCTCAGAAG ATAGGAATCG AATGAAAACA CTTGGTAGAC GGGATTCAAG TGATGATTGG 
GAGATACCTG ATGGGCAGAT CACAGTGGGA CAGAGAATTG GATCCGGGTC ATTTGGGACA 
GTCTACAAGG GAAAGTGGCA TGGTGACGTG GCAGTGAAAA TGTTGAATGT GACAGCACCC 
ACACCTCAGC AGTTACAGGC CTTCAAAAAT GAAGTAGGAG TACTCAGGAA AACTCGACAT 
GTGAATATCC TACTCTTTAT GGGCTATTCA ACAAAGCCCC AACTGGCTAT TGTTACCCAG 
TGGTGTGAGG GCTCCAGCTT ATATCACCAT CTCCACATCA TTGAGACCAA ATTTGAGATG 
ATAAAGCTTA TAGATATTGC ACGGCAGACT GCACAGGGCA TGGATTACTT ACACGCCAAG 
TCAATCATCC ACAGAGACCT CAAGAGTAAT AATATTTTTC TTCATGAAGA CCTCACAGTA 
AAAATAGGTG ATTTTGGTCT AGCCACAGTG AAATCTCGAT GGAGTGGGTC CCATCAGTTT 
GAACAGTTGT CTGGATCCAT TTTGTGGATG GCACCAGAAG TGATCCGAAT GCAAGACAAA 
AACCCATATA GCTTCCAGTC AGATGTATAC GCATTTGGGA TTGTTCTATA TGAATTGATG 
ACAGGGCAGT TACCTTATTC AAACATCAAC AACAGGGACC AGATAATTTT TATGGTGGGA 
CGAGGATATC TTTCTCCAGA TCTCAGTAAG GTACGGAGTA ACTGTCCAAA AGCCATGAAG 
AGATTGATGG CAGAGTGCCT AAAAAAGAAA AGAGATGAGA GGCCACTCTT TCCCCAAATT 
CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA TTGCCAAAAA TTCACCGCAG TGCATCAGAA 
CCCTCCTTGA ATCGGGCTGG CTTCCAAACA GAGGATTTTA GTCTCTATGC TTGCGCTTCT 
CCAAAAACAC CCATCCAGGC AGGGGGATAC GGAGAATTTG CAGCCTTCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629607.1
CDS	145..1998
Translation	MLDFHGISLTCAAVASSKVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASIAETALTSGSSPSAPPSDSPGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRSDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Target ORF information:

RefSeq Version	XM_005629550.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629550.3

ORF Insert Sequence:

ATGCTGGATT TTCATGGAAT AAGTTTGACC TGTGCTGCAG TGGCCTCCAG CAAGGTACCC 
GCAAGATGTG GAGTTACAGT CCGGGACAGT CTAAAGAAAG CTCTGATGAT GAGAGGTCTA 
ATCCCAGAGT GCTGTGCTGT TTACAGAATT CAGGATGGAG AGAAGAAACC GATTGGCTGG 
GACACTGATA TTTCCTGGCT CACTGGAGAG GAATTGCATG TAGAAGTGTT GGAAAATGTT 
CCGCTTACCA CACACAACTT TGTACGGAAA ACTTTTTTCA CCTTAGCATT TTGTGACTTT 
TGTCGAAAGC TGCTTTTCCA GGGTTTTCGC TGTCAAACAT GTGGTTATAA ATTTCACCAG 
CGTTGTAGTA CAGAGGTTCC ACTGATGTGT GTTAATTATG ACCAACTTGA TTTGCTGTTT 
GTCTCCAAGT TCTTTGAACA CCACCCAATA CCACAGGAGG AGGCCTCCAT AGCAGAGACT 
GCCCTTACGT CTGGATCATC CCCTTCTGCT CCCCCCTCCG ATTCTCCTGG GCCCCCAATT 
CTGACCAGTC CGTCTCCTTC AAAATCCATT CCAATTCCAC AGCCTTTCCG ACCAGCAGAT 
GAAGATCATC GAAATCAGTT TGGACAACGA GACCGGTCCT CATCAGCTCC AAATGTGCAT 
ATAAACACAA TAGAACCCGT CAACATTGAT GACTTGATTA GAGACCAAGG GTTTCGTAGT 
GATGGAGGAT CAACCACAGG TTTGTCTGCC ACCCCCCCTG CCTCATTGCC TGGCTCACTC 
ACTAATGTAA AAGCATTACA GAAATCTCCA GGACCTCAGC GGGAAAGAAA ATCATCTTCA 
TCCTCAGAAG ATAGGAATCG AATGAAAACA CTTGGTAGAC GGGATTCAAG TGATGATTGG 
GAGATACCTG ATGGGCAGAT CACAGTGGGA CAGAGAATTG GATCCGGGTC ATTTGGGACA 
GTCTACAAGG GAAAGTGGCA TGGTGACGTG GCAGTGAAAA TGTTGAATGT GACAGCACCC 
ACACCTCAGC AGTTACAGGC CTTCAAAAAT GAAGTAGGAG TACTCAGGAA AACTCGACAT 
GTGAATATCC TACTCTTTAT GGGCTATTCA ACAAAGCCCC AACTGGCTAT TGTTACCCAG 
TGGTGTGAGG GCTCCAGCTT ATATCACCAT CTCCACATCA TTGAGACCAA ATTTGAGATG 
ATAAAGCTTA TAGATATTGC ACGGCAGACT GCACAGGGCA TGGATTACTT ACACGCCAAG 
TCAATCATCC ACAGAGACCT CAAGAGTAAT AATATTTTTC TTCATGAAGA CCTCACAGTA 
AAAATAGGTG ATTTTGGTCT AGCCACAGTG AAATCTCGAT GGAGTGGGTC CCATCAGTTT 
GAACAGTTGT CTGGATCCAT TTTGTGGATG GCACCAGAAG TGATCCGAAT GCAAGACAAA 
AACCCATATA GCTTCCAGTC AGATGTATAC GCATTTGGGA TTGTTCTATA TGAATTGATG 
ACAGGGCAGT TACCTTATTC AAACATCAAC AACAGGGACC AGATAATTTT TATGGTGGGA 
CGAGGATATC TTTCTCCAGA TCTCAGTAAG GTACGGAGTA ACTGTCCAAA AGCCATGAAG 
AGATTGATGG CAGAGTGCCT AAAAAAGAAA AGAGATGAGA GGCCACTCTT TCCCCAAATT 
CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA TTGCCAAAAA TTCACCGCAG TGCATCAGAA 
CCCTCCTTGA ATCGGGCTGG CTTCCAAACA GAGGATTTTA GTCTCTATGC TTGCGCTTCT 
CCAAAAACAC CCATCCAGGC AGGGGGATAC GGAGAATTTG CAGCCTTCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_005629551.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629608.1 Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe17980
Clone ID Related Accession (Same CDS sequence)	XM_005629551.3 , XM_005629551.2
Accession Version	XM_005629551.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase B-raf isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629551.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAAGT TGACACAGGA ACATATAGAA GCCCTATTGG ACAAGTTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAACT AGATGCCCTC 
CAACAGCGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACGGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATATATCAC GGAGCAATCC CAAGTCACCA 
CAAAAACCTA TCGTTAGAGT CTTCCTGCCC AATAAACAGA GGACGGTGGT ACCCGCAAGA 
TGTGGAGTTA CAGTCCGGGA CAGTCTAAAG AAAGCTCTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTTTACAG AATTCAGGAT GGAGAGAAGA AACCGATTGG CTGGGACACT 
GATATTTCCT GGCTCACTGG AGAGGAATTG CATGTAGAAG TGTTGGAAAA TGTTCCGCTT 
ACCACACACA ACTTTGTACG GAAAACTTTT TTCACCTTAG CATTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT TCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAGGAGGCCT CCATAGCAGA GACTGCCCTT 
ACGTCTGGAT CATCCCCTTC TGCTCCCCCC TCCGATTCTC CTGGGCCCCC AATTCTGACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATT CCACAGCCTT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AGTTTGGACA ACGAGACCGG TCCTCATCAG CTCCAAATGT GCATATAAAC 
ACAATAGAAC CCGTCAACAT TGATGACTTG ATTAGAGACC AAGGGTTTCG TAGTGATGGA 
GGATCAACCA CAGGTTTGTC TGCCACCCCC CCTGCCTCAT TGCCTGGCTC ACTCACTAAT 
GTAAAAGCAT TACAGAAATC TCCAGGACCT CAGCGGGAAA GAAAATCATC TTCATCCTCA 
GAAGATAGGA ATCGAATGAA AACACTTGGT AGACGGGATT CAAGTGATGA TTGGGAGATA 
CCTGATGGGC AGATCACAGT GGGACAGAGA ATTGGATCCG GGTCATTTGG GACAGTCTAC 
AAGGGAAAGT GGCATGGTGA CGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACTCG ACATGTGAAT 
ATCCTACTCT TTATGGGCTA TTCAACAAAG CCCCAACTGG CTATTGTTAC CCAGTGGTGT 
GAGGGCTCCA GCTTATATCA CCATCTCCAC ATCATTGAGA CCAAATTTGA GATGATAAAG 
CTTATAGATA TTGCACGGCA GACTGCACAG GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGTGATTTTG GTCTAGCCAC AGTGAAATCT CGATGGAGTG GGTCCCATCA GTTTGAACAG 
TTGTCTGGAT CCATTTTGTG GATGGCACCA GAAGTGATCC GAATGCAAGA CAAAAACCCA 
TATAGCTTCC AGTCAGATGT ATACGCATTT GGGATTGTTC TATATGAATT GATGACAGGG 
CAGTTACCTT ATTCAAACAT CAACAACAGG GACCAGCTCA GATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629608.1
CDS	19..1866
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASTDTVTSSSSSSLSVLPSSLSVFQNPTDISRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASIAETALTSGSSPSAPPSDSPGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRSDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQLRS

Target ORF information:

RefSeq Version	XM_005629551.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629551.3

ORF Insert Sequence:

ATGATTAAGT TGACACAGGA ACATATAGAA GCCCTATTGG ACAAGTTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAACT AGATGCCCTC 
CAACAGCGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACGGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATATATCAC GGAGCAATCC CAAGTCACCA 
CAAAAACCTA TCGTTAGAGT CTTCCTGCCC AATAAACAGA GGACGGTGGT ACCCGCAAGA 
TGTGGAGTTA CAGTCCGGGA CAGTCTAAAG AAAGCTCTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTTTACAG AATTCAGGAT GGAGAGAAGA AACCGATTGG CTGGGACACT 
GATATTTCCT GGCTCACTGG AGAGGAATTG CATGTAGAAG TGTTGGAAAA TGTTCCGCTT 
ACCACACACA ACTTTGTACG GAAAACTTTT TTCACCTTAG CATTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT TCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAGGAGGCCT CCATAGCAGA GACTGCCCTT 
ACGTCTGGAT CATCCCCTTC TGCTCCCCCC TCCGATTCTC CTGGGCCCCC AATTCTGACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATT CCACAGCCTT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AGTTTGGACA ACGAGACCGG TCCTCATCAG CTCCAAATGT GCATATAAAC 
ACAATAGAAC CCGTCAACAT TGATGACTTG ATTAGAGACC AAGGGTTTCG TAGTGATGGA 
GGATCAACCA CAGGTTTGTC TGCCACCCCC CCTGCCTCAT TGCCTGGCTC ACTCACTAAT 
GTAAAAGCAT TACAGAAATC TCCAGGACCT CAGCGGGAAA GAAAATCATC TTCATCCTCA 
GAAGATAGGA ATCGAATGAA AACACTTGGT AGACGGGATT CAAGTGATGA TTGGGAGATA 
CCTGATGGGC AGATCACAGT GGGACAGAGA ATTGGATCCG GGTCATTTGG GACAGTCTAC 
AAGGGAAAGT GGCATGGTGA CGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACTCG ACATGTGAAT 
ATCCTACTCT TTATGGGCTA TTCAACAAAG CCCCAACTGG CTATTGTTAC CCAGTGGTGT 
GAGGGCTCCA GCTTATATCA CCATCTCCAC ATCATTGAGA CCAAATTTGA GATGATAAAG 
CTTATAGATA TTGCACGGCA GACTGCACAG GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGTGATTTTG GTCTAGCCAC AGTGAAATCT CGATGGAGTG GGTCCCATCA GTTTGAACAG 
TTGTCTGGAT CCATTTTGTG GATGGCACCA GAAGTGATCC GAATGCAAGA CAAAAACCCA 
TATAGCTTCC AGTCAGATGT ATACGCATTT GGGATTGTTC TATATGAATT GATGACAGGG 
CAGTTACCTT ATTCAAACAT CAACAACAGG GACCAGCTCA GATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_005629551.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629608.1 Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe17980
Clone ID Related Accession (Same CDS sequence)	XM_005629551.3 , XM_005629551.2
Accession Version	XM_005629551.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase B-raf isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAAGT TGACACAGGA ACATATAGAA GCCCTATTGG ACAAGTTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAACT AGATGCCCTC 
CAACAGCGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACGGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATATATCAC GGAGCAATCC CAAGTCACCA 
CAAAAACCTA TCGTTAGAGT CTTCCTGCCC AATAAACAGA GGACGGTGGT ACCCGCAAGA 
TGTGGAGTTA CAGTCCGGGA CAGTCTAAAG AAAGCTCTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTTTACAG AATTCAGGAT GGAGAGAAGA AACCGATTGG CTGGGACACT 
GATATTTCCT GGCTCACTGG AGAGGAATTG CATGTAGAAG TGTTGGAAAA TGTTCCGCTT 
ACCACACACA ACTTTGTACG GAAAACTTTT TTCACCTTAG CATTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT TCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAGGAGGCCT CCATAGCAGA GACTGCCCTT 
ACGTCTGGAT CATCCCCTTC TGCTCCCCCC TCCGATTCTC CTGGGCCCCC AATTCTGACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATT CCACAGCCTT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AGTTTGGACA ACGAGACCGG TCCTCATCAG CTCCAAATGT GCATATAAAC 
ACAATAGAAC CCGTCAACAT TGATGACTTG ATTAGAGACC AAGGGTTTCG TAGTGATGGA 
GGATCAACCA CAGGTTTGTC TGCCACCCCC CCTGCCTCAT TGCCTGGCTC ACTCACTAAT 
GTAAAAGCAT TACAGAAATC TCCAGGACCT CAGCGGGAAA GAAAATCATC TTCATCCTCA 
GAAGATAGGA ATCGAATGAA AACACTTGGT AGACGGGATT CAAGTGATGA TTGGGAGATA 
CCTGATGGGC AGATCACAGT GGGACAGAGA ATTGGATCCG GGTCATTTGG GACAGTCTAC 
AAGGGAAAGT GGCATGGTGA CGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACTCG ACATGTGAAT 
ATCCTACTCT TTATGGGCTA TTCAACAAAG CCCCAACTGG CTATTGTTAC CCAGTGGTGT 
GAGGGCTCCA GCTTATATCA CCATCTCCAC ATCATTGAGA CCAAATTTGA GATGATAAAG 
CTTATAGATA TTGCACGGCA GACTGCACAG GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGTGATTTTG GTCTAGCCAC AGTGAAATCT CGATGGAGTG GGTCCCATCA GTTTGAACAG 
TTGTCTGGAT CCATTTTGTG GATGGCACCA GAAGTGATCC GAATGCAAGA CAAAAACCCA 
TATAGCTTCC AGTCAGATGT ATACGCATTT GGGATTGTTC TATATGAATT GATGACAGGG 
CAGTTACCTT ATTCAAACAT CAACAACAGG GACCAGCTCA GATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629608.1
CDS	19..1866
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASTDTVTSSSSSSLSVLPSSLSVFQNPTDISRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASIAETALTSGSSPSAPPSDSPGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRSDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQLRS

Target ORF information:

RefSeq Version	XM_005629551.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629551.2

ORF Insert Sequence:

ATGATTAAGT TGACACAGGA ACATATAGAA GCCCTATTGG ACAAGTTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAACT AGATGCCCTC 
CAACAGCGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACGGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATATATCAC GGAGCAATCC CAAGTCACCA 
CAAAAACCTA TCGTTAGAGT CTTCCTGCCC AATAAACAGA GGACGGTGGT ACCCGCAAGA 
TGTGGAGTTA CAGTCCGGGA CAGTCTAAAG AAAGCTCTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTTTACAG AATTCAGGAT GGAGAGAAGA AACCGATTGG CTGGGACACT 
GATATTTCCT GGCTCACTGG AGAGGAATTG CATGTAGAAG TGTTGGAAAA TGTTCCGCTT 
ACCACACACA ACTTTGTACG GAAAACTTTT TTCACCTTAG CATTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT TCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAGGAGGCCT CCATAGCAGA GACTGCCCTT 
ACGTCTGGAT CATCCCCTTC TGCTCCCCCC TCCGATTCTC CTGGGCCCCC AATTCTGACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATT CCACAGCCTT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AGTTTGGACA ACGAGACCGG TCCTCATCAG CTCCAAATGT GCATATAAAC 
ACAATAGAAC CCGTCAACAT TGATGACTTG ATTAGAGACC AAGGGTTTCG TAGTGATGGA 
GGATCAACCA CAGGTTTGTC TGCCACCCCC CCTGCCTCAT TGCCTGGCTC ACTCACTAAT 
GTAAAAGCAT TACAGAAATC TCCAGGACCT CAGCGGGAAA GAAAATCATC TTCATCCTCA 
GAAGATAGGA ATCGAATGAA AACACTTGGT AGACGGGATT CAAGTGATGA TTGGGAGATA 
CCTGATGGGC AGATCACAGT GGGACAGAGA ATTGGATCCG GGTCATTTGG GACAGTCTAC 
AAGGGAAAGT GGCATGGTGA CGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACTCG ACATGTGAAT 
ATCCTACTCT TTATGGGCTA TTCAACAAAG CCCCAACTGG CTATTGTTAC CCAGTGGTGT 
GAGGGCTCCA GCTTATATCA CCATCTCCAC ATCATTGAGA CCAAATTTGA GATGATAAAG 
CTTATAGATA TTGCACGGCA GACTGCACAG GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGTGATTTTG GTCTAGCCAC AGTGAAATCT CGATGGAGTG GGTCCCATCA GTTTGAACAG 
TTGTCTGGAT CCATTTTGTG GATGGCACCA GAAGTGATCC GAATGCAAGA CAAAAACCCA 
TATAGCTTCC AGTCAGATGT ATACGCATTT GGGATTGTTC TATATGAATT GATGACAGGG 
CAGTTACCTT ATTCAAACAT CAACAACAGG GACCAGCTCA GATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_022403735.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259443.1 Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe293847
Clone ID Related Accession (Same CDS sequence)	XM_022403735.1
Accession Version	XM_022403735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase B-raf isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAAGT TGACACAGGA ACATATAGAA GCCCTATTGG ACAAGTTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAACT AGATGCCCTC 
CAACAGCGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACGGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATATATCAC GGAGCAATCC CAAGTCACCA 
CAAAAACCTA TCGTTAGAGT CTTCCTGCCC AATAAACAGA GGACGGTGGT ACCCGCAAGA 
TGTGGAGTTA CAGTCCGGGA CAGTCTAAAG AAAGCTCTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTTTACAG AATTCAGGAT GGAGAGAAGA AACCGATTGG CTGGGACACT 
GATATTTCCT GGCTCACTGG AGAGGAATTG CATGTAGAAG TGTTGGAAAA TGTTCCGCTT 
ACCACACACA ACTTTGTACG GAAAACTTTT TTCACCTTAG CATTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT TCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAGGAGGCCT CCATAGCAGA GACTGCCCTT 
ACGTCTGGAT CATCCCCTTC TGCTCCCCCC TCCGATTCTC CTGGGCCCCC AATTCTGACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATT CCACAGCCTT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AGTTTGGACA ACGAGACCGG TCCTCATCAG CTCCAAATGT GCATATAAAC 
ACAATAGAAC CCGTCAACAT TGATGACTTG ATTAGAGACC AAGGGTTTCG TAGTGATGGA 
GGATCAACCA CAGGTTTGTC TGCCACCCCC CCTGCCTCAT TGCCTGGCTC ACTCACTAAT 
GTAAAAGCAT TACAGAAATC TCCAGGACCT CAGCGGGAAA GAAAATCATC TTCATCCTCA 
GAAGATAGGA ATCGAATGAA AACACTTGGT AGACGGGATT CAAGTGATGA TTGGGAGATA 
CCTGATGGGC AGATCACAGT GGGACAGAGA ATTGGATCCG GGTCATTTGG GACAGTCTAC 
AAGGGAAAGT GGCATGGTGA CGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACTCG ACATGTGAAT 
ATCCTACTCT TTATGGGCTA TTCAACAAAG CCCCAACTGG CTATTGTTAC CCAGTGGTGT 
GAGGGCTCCA GCTTATATCA CCATCTCCAC ATCATTGAGA CCAAATTTGA GATGATAAAG 
CTTATAGATA TTGCACGGCA GACTGCACAG GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGTGATTTTG GTCTAGCCAC AGTGAAATCT CGATGGAGTG GGTCCCATCA GTTTGAACAG 
TTGTCTGGAT CCATTTTGTG GATGGAATGG AAATTGGAAT TGATTCTTGA TAGAGCTAAT 
CCTGGGAAGG ATTCAAATAG CAATGGCAGT ATTTTGTACA GAAAGAGGAT GGCTGATAAA 
ATTATAAAAT CTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259443.1
CDS	19..1836
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASTDTVTSSSSSSLSVLPSSLSVFQNPTDISRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASIAETALTSGSSPSAPPSDSPGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRSDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMEWKLELILDRANPGKDSNSNGSILYRKRMADKIIKSS

Target ORF information:

RefSeq Version	XM_022403735.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403735.1

ORF Insert Sequence:

ATGATTAAGT TGACACAGGA ACATATAGAA GCCCTATTGG ACAAGTTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAACT AGATGCCCTC 
CAACAGCGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACGGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATATATCAC GGAGCAATCC CAAGTCACCA 
CAAAAACCTA TCGTTAGAGT CTTCCTGCCC AATAAACAGA GGACGGTGGT ACCCGCAAGA 
TGTGGAGTTA CAGTCCGGGA CAGTCTAAAG AAAGCTCTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTTTACAG AATTCAGGAT GGAGAGAAGA AACCGATTGG CTGGGACACT 
GATATTTCCT GGCTCACTGG AGAGGAATTG CATGTAGAAG TGTTGGAAAA TGTTCCGCTT 
ACCACACACA ACTTTGTACG GAAAACTTTT TTCACCTTAG CATTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT TCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAGGAGGCCT CCATAGCAGA GACTGCCCTT 
ACGTCTGGAT CATCCCCTTC TGCTCCCCCC TCCGATTCTC CTGGGCCCCC AATTCTGACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATT CCACAGCCTT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AGTTTGGACA ACGAGACCGG TCCTCATCAG CTCCAAATGT GCATATAAAC 
ACAATAGAAC CCGTCAACAT TGATGACTTG ATTAGAGACC AAGGGTTTCG TAGTGATGGA 
GGATCAACCA CAGGTTTGTC TGCCACCCCC CCTGCCTCAT TGCCTGGCTC ACTCACTAAT 
GTAAAAGCAT TACAGAAATC TCCAGGACCT CAGCGGGAAA GAAAATCATC TTCATCCTCA 
GAAGATAGGA ATCGAATGAA AACACTTGGT AGACGGGATT CAAGTGATGA TTGGGAGATA 
CCTGATGGGC AGATCACAGT GGGACAGAGA ATTGGATCCG GGTCATTTGG GACAGTCTAC 
AAGGGAAAGT GGCATGGTGA CGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACTCG ACATGTGAAT 
ATCCTACTCT TTATGGGCTA TTCAACAAAG CCCCAACTGG CTATTGTTAC CCAGTGGTGT 
GAGGGCTCCA GCTTATATCA CCATCTCCAC ATCATTGAGA CCAAATTTGA GATGATAAAG 
CTTATAGATA TTGCACGGCA GACTGCACAG GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGTGATTTTG GTCTAGCCAC AGTGAAATCT CGATGGAGTG GGTCCCATCA GTTTGAACAG 
TTGTCTGGAT CCATTTTGTG GATGGAATGG AAATTGGAAT TGATTCTTGA TAGAGCTAAT 
CCTGGGAAGG ATTCAAATAG CAATGGCAGT ATTTTGTACA GAAAGAGGAT GGCTGATAAA 
ATTATAAAAT CTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_022403736.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259444.1 Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA.

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CloneID	OCe293848
Clone ID Related Accession (Same CDS sequence)	XM_022403736.1
Accession Version	XM_022403736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase B-raf isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAAGT TGACACAGGA ACATATAGAA GCCCTATTGG ACAAGTTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAACT AGATGCCCTC 
CAACAGCGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACGGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATATATCAC GGAGCAATCC CAAGTCACCA 
CAAAAACCTA TCGTTAGAGT CTTCCTGCCC AATAAACAGA GGACGGTGGT ACCCGCAAGA 
TGTGGAGTTA CAGTCCGGGA CAGTCTAAAG AAAGCTCTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTTTACAG AATTCAGGAT GGAGAGAAGA AACCGATTGG CTGGGACACT 
GATATTTCCT GGCTCACTGG AGAGGAATTG CATGTAGAAG TGTTGGAAAA TGTTCCGCTT 
ACCACACACA ACTTTGTACG GAAAACTTTT TTCACCTTAG CATTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT TCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAGGAGGCCT CCATAGCAGA GACTGCCCTT 
ACGTCTGGAT CATCCCCTTC TGCTCCCCCC TCCGATTCTC CTGGGCCCCC AATTCTGACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATT CCACAGCCTT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AGTTTGGACA ACGAGACCGG TCCTCATCAG CTCCAAATGT GCATATAAAC 
ACAATAGAAC CCGTCAACAT TGATGACTTG ATTAGAGACC AAGGGTTTCG TAGTGATGGA 
GGATCAACCA CAGGTTTGTC TGCCACCCCC CCTGCCTCAT TGCCTGGCTC ACTCACTAAT 
GTAAAAGCAT TACAGAAATC TCCAGGACCT CAGCGGGAAA GAAAATCATC TTCATCCTCA 
GAAGATAGGA ATCGAATGAA AACACTTGGT AGACGGGATT CAAGTGATGA TTGGGAGATA 
CCTGATGGGC AGATCACAGT GGGACAGAGA ATTGGATCCG GGTCATTTGG GACAGTCTAC 
AAGGGAAAGT GGCATGGTGA CGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACTCG ACATGTGAAT 
ATCCTACTCT TTATGGGCTA TTCAACAAAG CCCCAACTGG CTATTGTTAC CCAGTGGTGT 
GAGGGCTCCA GCTTATATCA CCATCTCCAC ATCATTGAGA CCAAATTTGA GATGATAAAG 
CTTATAGATA TTGCACGGCA GACTGCACAG GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAGTATC CTTCCTGAAA TTTGTCTGCG AAGTTTGAAA 
ACATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259444.1
CDS	19..1647
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASTDTVTSSSSSSLSVLPSSLSVFQNPTDISRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASIAETALTSGSSPSAPPSDSPGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRSDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNSILPEICLRSLKTS

Target ORF information:

RefSeq Version	XM_022403736.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403736.1

ORF Insert Sequence:

ATGATTAAGT TGACACAGGA ACATATAGAA GCCCTATTGG ACAAGTTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAACT AGATGCCCTC 
CAACAGCGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACGGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATATATCAC GGAGCAATCC CAAGTCACCA 
CAAAAACCTA TCGTTAGAGT CTTCCTGCCC AATAAACAGA GGACGGTGGT ACCCGCAAGA 
TGTGGAGTTA CAGTCCGGGA CAGTCTAAAG AAAGCTCTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTTTACAG AATTCAGGAT GGAGAGAAGA AACCGATTGG CTGGGACACT 
GATATTTCCT GGCTCACTGG AGAGGAATTG CATGTAGAAG TGTTGGAAAA TGTTCCGCTT 
ACCACACACA ACTTTGTACG GAAAACTTTT TTCACCTTAG CATTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT TCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAGGAGGCCT CCATAGCAGA GACTGCCCTT 
ACGTCTGGAT CATCCCCTTC TGCTCCCCCC TCCGATTCTC CTGGGCCCCC AATTCTGACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATT CCACAGCCTT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AGTTTGGACA ACGAGACCGG TCCTCATCAG CTCCAAATGT GCATATAAAC 
ACAATAGAAC CCGTCAACAT TGATGACTTG ATTAGAGACC AAGGGTTTCG TAGTGATGGA 
GGATCAACCA CAGGTTTGTC TGCCACCCCC CCTGCCTCAT TGCCTGGCTC ACTCACTAAT 
GTAAAAGCAT TACAGAAATC TCCAGGACCT CAGCGGGAAA GAAAATCATC TTCATCCTCA 
GAAGATAGGA ATCGAATGAA AACACTTGGT AGACGGGATT CAAGTGATGA TTGGGAGATA 
CCTGATGGGC AGATCACAGT GGGACAGAGA ATTGGATCCG GGTCATTTGG GACAGTCTAC 
AAGGGAAAGT GGCATGGTGA CGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACTCG ACATGTGAAT 
ATCCTACTCT TTATGGGCTA TTCAACAAAG CCCCAACTGG CTATTGTTAC CCAGTGGTGT 
GAGGGCTCCA GCTTATATCA CCATCTCCAC ATCATTGAGA CCAAATTTGA GATGATAAAG 
CTTATAGATA TTGCACGGCA GACTGCACAG GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAGTATC CTTCCTGAAA TTTGTCTGCG AAGTTTGAAA 
ACATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_014119889.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975364.1 Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe40466
Clone ID Related Accession (Same CDS sequence)	XM_014119889.1
Accession Version	XM_014119889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase B-raf isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAAGT TGACACAGGA ACATATAGAA GCCCTATTGG ACAAGTTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAACT AGATGCCCTC 
CAACAGCGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACGGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATATATCAC GGAGCAATCC CAAGTCACCA 
CAAAAACCTA TCGTTAGAGT CTTCCTGCCC AATAAACAGA GGACGGTGGT ACCCGCAAGA 
TGTGGAGTTA CAGTCCGGGA CAGTCTAAAG AAAGCTCTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTTTACAG AATTCAGGAT GGAGAGAAGA AACCGATTGG CTGGGACACT 
GATATTTCCT GGCTCACTGG AGAGGAATTG CATGTAGAAG TGTTGGAAAA TGTTCCGCTT 
ACCACACACA ACTTTGTACG GAAAACTTTT TTCACCTTAG CATTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT TCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAGGAGGCCT CCATAGCAGA GACTGCCCTT 
ACGTCTGGAT CATCCCCTTC TGCTCCCCCC TCCGATTCTC CTGGGCCCCC AATTCTGACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATT CCACAGCCTT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AGTTTGGACA ACGAGACCGG TCCTCATCAG CTCCAAATGT GCATATAAAC 
ACAATAGAAC CCGTCAACAT TGATGACTTG ATTAGAGACC AAGGGTTTCG TAGTGATGGA 
GGATCAACCA CAGGTTTGTC TGCCACCCCC CCTGCCTCAT TGCCTGGCTC ACTCACTAAT 
GTAAAAGCAT TACAGAAATC TCCAGGACCT CAGCGGGAAA GAAAATCATC TTCATCCTCA 
GAAGATAGGA ATCGAATGAA AACACTTGGT AGACGGGATT CAAGTGATGA TTGGGAGATA 
CCTGATGGGC AGATCACAGT GGGACAGAGA ATTGGATCCG GGTCATTTGG GACAGTCTAC 
AAGGGAAAGT GGCATGGTGA CGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACTCG ACATGTGAAT 
ATCCTACTCT TTATGGGCTA TTCAACAAAG CCCCAACTGG CTATTGTTAC CCAGTGGTGT 
GAGGGCTCCA GCTTATATCA CCATCTCCAC ATCATTGAGA CCAAATTTGA GATGATAAAG 
CTTATAGATA TTGCACGGCA GACTGCACAG GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGTGATTTTG GTCTAGCCAC AGTGAAATCT CGATGGAGTG GGTCCCATCA GTTTGAACAG 
TTGTCTGGAT CCATTTTGTG GATGGCACCA GAAGTGATCC GAATGCAAGA CAAAAACCCA 
TATAGCTTCC AGTCAGATGT ATACGCATTT GGGATTGTTC TATATGAATT GATGACAGGG 
CAGTTACCTT ATTCAAACAT CAACAACAGG GACCAGATAA TTTTTATGGT GGGACGAGGA 
TATCTTTCTC CAGATCTCAG TAAGGTACGG AGTAACTGTC CAAAAGCCAT GAAGAGATTG 
ATGGCAGAGT GCCTAAAAAA GAAAAGAGAT GAGAGGCCAC TCTTTCCCCA AATTCTCGCC 
TCTATTGAGC TGCTGGCCCG CTCATTGCCA AAAATTCACC GCAGTGCATC AGAACCCTCC 
TTGAATCGGG CTGGCTTCCA AACAGAGGAT TTTAGTCTCT ATGCTTGCGC TTCTCCAAAA 
ACACCCATCC AGGCAGGGGG ATACGGAGAA TTTGCAGCCT TCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975364.1
CDS	19..2166
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASTDTVTSSSSSSLSVLPSSLSVFQNPTDISRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASIAETALTSGSSPSAPPSDSPGPPILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRSDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Target ORF information:

RefSeq Version	XM_014119889.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119889.1

ORF Insert Sequence:

ATGATTAAGT TGACACAGGA ACATATAGAA GCCCTATTGG ACAAGTTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAACT AGATGCCCTC 
CAACAGCGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACGGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATATATCAC GGAGCAATCC CAAGTCACCA 
CAAAAACCTA TCGTTAGAGT CTTCCTGCCC AATAAACAGA GGACGGTGGT ACCCGCAAGA 
TGTGGAGTTA CAGTCCGGGA CAGTCTAAAG AAAGCTCTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTTTACAG AATTCAGGAT GGAGAGAAGA AACCGATTGG CTGGGACACT 
GATATTTCCT GGCTCACTGG AGAGGAATTG CATGTAGAAG TGTTGGAAAA TGTTCCGCTT 
ACCACACACA ACTTTGTACG GAAAACTTTT TTCACCTTAG CATTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT TCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAGGAGGCCT CCATAGCAGA GACTGCCCTT 
ACGTCTGGAT CATCCCCTTC TGCTCCCCCC TCCGATTCTC CTGGGCCCCC AATTCTGACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATT CCACAGCCTT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AGTTTGGACA ACGAGACCGG TCCTCATCAG CTCCAAATGT GCATATAAAC 
ACAATAGAAC CCGTCAACAT TGATGACTTG ATTAGAGACC AAGGGTTTCG TAGTGATGGA 
GGATCAACCA CAGGTTTGTC TGCCACCCCC CCTGCCTCAT TGCCTGGCTC ACTCACTAAT 
GTAAAAGCAT TACAGAAATC TCCAGGACCT CAGCGGGAAA GAAAATCATC TTCATCCTCA 
GAAGATAGGA ATCGAATGAA AACACTTGGT AGACGGGATT CAAGTGATGA TTGGGAGATA 
CCTGATGGGC AGATCACAGT GGGACAGAGA ATTGGATCCG GGTCATTTGG GACAGTCTAC 
AAGGGAAAGT GGCATGGTGA CGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACTCG ACATGTGAAT 
ATCCTACTCT TTATGGGCTA TTCAACAAAG CCCCAACTGG CTATTGTTAC CCAGTGGTGT 
GAGGGCTCCA GCTTATATCA CCATCTCCAC ATCATTGAGA CCAAATTTGA GATGATAAAG 
CTTATAGATA TTGCACGGCA GACTGCACAG GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGTGATTTTG GTCTAGCCAC AGTGAAATCT CGATGGAGTG GGTCCCATCA GTTTGAACAG 
TTGTCTGGAT CCATTTTGTG GATGGCACCA GAAGTGATCC GAATGCAAGA CAAAAACCCA 
TATAGCTTCC AGTCAGATGT ATACGCATTT GGGATTGTTC TATATGAATT GATGACAGGG 
CAGTTACCTT ATTCAAACAT CAACAACAGG GACCAGATAA TTTTTATGGT GGGACGAGGA 
TATCTTTCTC CAGATCTCAG TAAGGTACGG AGTAACTGTC CAAAAGCCAT GAAGAGATTG 
ATGGCAGAGT GCCTAAAAAA GAAAAGAGAT GAGAGGCCAC TCTTTCCCCA AATTCTCGCC 
TCTATTGAGC TGCTGGCCCG CTCATTGCCA AAAATTCACC GCAGTGCATC AGAACCCTCC 
TTGAATCGGG CTGGCTTCCA AACAGAGGAT TTTAGTCTCT ATGCTTGCGC TTCTCCAAAA 
ACACCCATCC AGGCAGGGGG ATACGGAGAA TTTGCAGCCT TCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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BRAF cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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