ALPL cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALPL
Entrez Gene ID	403548
Full Name	alkaline phosphatase, liver/bone/kidney
Synonyms	ALP
General protein information	Preferred Names alkaline phosphatase, liver/bone/kidney Names alkaline phosphatase, tissue-nonspecific isozyme tissue non-specific alkaline phosphatase
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005617214.3	XP_005617271.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
XM_005617212.2	XP_005617269.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
XM_005617213.2	XP_005617270.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
XM_005617213.3	XP_005617270.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
XM_005617214.2	XP_005617271.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
NM_001197137.1	NP_001184066.1	alkaline phosphatase, tissue-nonspecific isozyme
XM_005617212.3	XP_005617269.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ALPL	NP_789828.2
Canis lupus familiaris (dog)	ALPL	NP_001184066.1
Rattus norvegicus (Norway rat)	Alpl	NP_037191.1
Gallus gallus (chicken)	ALPL	NP_990691.1
Drosophila melanogaster (fruit fly)	CG8147	NP_649897.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001684	XP_321411.3
Homo sapiens (human)	ALPL	NP_000469.3
Macaca mulatta (Rhesus monkey)	ALPL	XP_001109717.2
Pan troglodytes (chimpanzee)	ALPL	XP_003307915.1
Xenopus tropicalis (tropical clawed frog)	LOC100485467	XP_002943485.2
Mus musculus (house mouse)	Alpl	NP_001274101.1
Danio rerio (zebrafish)	alp3	NP_001265768.1

Gene Ontology
Bibliography

Related articles in PubMed

The Association of Endothelin-1 Signaling with Bone Alkaline Phosphatase Expression and Protumorigenic Activities in Canine Osteosarcoma.
Neumann ZL, Pondenis HC, Masyr A, Byrum ML, Wycislo KL, Fan TM
Journal of veterinary internal medicine29(6)1584-94(2015 Nov-Dec)

Association between absolute tumor burden and serum bone-specific alkaline phosphatase in canine appendicular osteosarcoma.
Sternberg RA, Pondenis HC, Yang X, Mitchell MA, O'Brien RT, Garrett LD, Helferich WG, Hoffmann WE, Fan TM
Journal of veterinary internal medicine27(4)955-63(2013 Jul-Aug)

The influence of sex on biochemical markers of bone turnover in dogs.
Belić M, Kušec V, Svetina A, Grizelj J, Robić M, Vrbanac Z, Benić M, Turk R
Research in veterinary science93(2)918-20(2012 Oct)

Wnt/β-catenin expression does not correlate with serum alkaline phosphatase concentration in canine osteosarcoma patients.
Piskun CM, Muthuswamy A, Huelsmeyer MK, Thompson V, Stein TJ
PloS one6(10)e26106(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ALPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe00229	NM_001197137.1 Latest version!	Canis lupus familiaris alkaline phosphatase, biomineralization associated (ALPL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe02910	XM_005617212.3 Latest version!	Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe02910	XM_005617213.3 Latest version!	Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe02910	XM_005617214.3 Latest version!	Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OCe02910	XM_005617213.2	Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe02910	XM_005617214.2	Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe02910	XM_005617212.2	Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALPL ( NM_001197137.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001184066.1 Canis lupus familiaris alkaline phosphatase, biomineralization associated (ALPL), mRNA.

Price & Availability↑

CloneID	OCe00229
Clone ID Related Accession (Same CDS sequence)	NM_001197137.1
Accession Version	NM_001197137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1540224000000
Organism	Canis lupus familiaris(dog)
Product	alkaline phosphatase, tissue-nonspecific isozyme
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03001783.1. On Oct 8, 2010 this sequence version replaced XM_535374.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF540075.1, AF250846.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGGGGAGCT GCCTCCCACC CCTGCCAGAT AACTGCCTCT CTGTCTATAC AGAGAAAGAG 
AAAGACCCCA AGTATTGGAG AGACCAAGCT CAACAGACCC TGAAATACGC CCTCAGGCTT 
CAGAATCTCA ACACCAACGT GGCAAAGAAT GTCATCATGT TCCTGGGAGA TGGGATGGGT 
GTCTCCACGG TGACGGCCAC TCGCATCCTC AAGGGTCAGC TCCACCACAA CCCCGGGGAG 
GAGACCAGGC TGGAGATGGA CAAGTTCCCT TACGTGGCCC TCTCCAAGAC ATACAACACC 
AACGCTCAGG TCCCTGACAG CGCAGGCACT GCCACCGCCT ACTTGTGTGG AGTGAAGGCC 
AACGAGGGCA CGGTGGGCGT GAGTGCCGCG ACCCAGCGCA CCCAGTGCAA CACGACTCAG 
GGAAACGAGG TCACCTCCAT CCTGCGCTGG GCCAAGGACG CTGGGAAATC TGTGGGCATA 
GTGACCACCA CGAGGGTGAA CCACGCCACC CCCAGTGCCG CCTACGCCCA CTCCGCTGAC 
CGGGACTGGT ACTCGGACAA CGAGATGCCC CCGGAGGCCC TAAGCCAGGG CTGCAAGGAC 
ATTGCCTACC AGCTCATGCA CAACGTCAAG GACATCGAGG TGATCATGGG GGGCGGCCGG 
AAGTACATGT TCCCCAAGAA TAGAACAGAT GTGGAGTATG AGATGGACGA GAAGTCCAGG 
GGCACGAGGC TGGATGGCCT GAACCTCATC GACATCTGGA AGAACTTCAA ACCGAGACAC 
AAGCACTCTC ACTACGTCTG GAACCGCACG GAACTCCTGG CCCTCGACCC CTACACCGTG 
GACTACCTCT TGGGTCTCTT TGAGCCGGGG GACATGCAGT ACGAGCTGAA CAGGAACAAC 
GTGACTGACC CGTCACTCTC CGAGATGGTG GAAATAGCCA TCAAGATTCT GAGCAAGAAC 
CCCAGAGGCT TCTTCTTGCT GGTGGAAGGA GGCAGGATTG ACCACGGGCA TCACGAGGGC 
AAGGCCAAGC AGGCGCTGCA CGAGGCAGTG GAGATGGACC GGGCAATTGG GAAGGCAGGC 
GTCATGACCT CCTTGGAAGA CACGCTGACC GTCGTCACTG CGGACCACTC CCACGTCTTC 
ACCTTTGGCG GGTACACCCC CCGGGGCAAC TCTATCTTTG GTCTGGCCCC CATGGTGAGT 
GACACGGACA AGAAGCCCTT CACGGCCATC CTGTATGGCA ACGGGCCTGG CTACAAGGTG 
GTGGGCGGTG AGCGAGAGAA CGTCTCCATG GTGGACTACG CTCACAACAA CTACCAGGCG 
CAGTCCGCGG TGCCCCTGCG CCACGAGACC CACGGTGGGG AGGACGTAGC AGTCTTCGCC 
AAGGGTCCCA TGGCACACCT GCTGCACGGC GTCCACGAGC AGAACTACAT CCCCCACGTG 
ATGGCTTATG CGGCCTGCAT CGGTGCCAAC CAGGACCACT GTGCCTCAGC CAGCTCGGCA 
GGTGGCCCTT CTTCTCCAGG TCCCCTGCTC CTCCTGCTGG CCCTGCTCCC CGTGGGCATC 
CTATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001184066.1
CDS	169..1737
Translation	MGSCLPPLPDNCLSVYTEKEKDPKYWRDQAQQTLKYALRLQNLNTNVAKNVIMFLGDGMGVSTVTATRILKGQLHHNPGEETRLEMDKFPYVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATQRTQCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNVKDIEVIMGGGRKYMFPKNRTDVEYEMDEKSRGTRLDGLNLIDIWKNFKPRHKHSHYVWNRTELLALDPYTVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVEIAIKILSKNPRGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGKAGVMTSLEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYAACIGANQDHCASASSAGGPSSPGPLLLLLALLPVGILF

Target ORF information:

RefSeq Version	NM_001197137.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris alkaline phosphatase, biomineralization associated (ALPL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001197137.1

ORF Insert Sequence:

ATGGGGAGCT GCCTCCCACC CCTGCCAGAT AACTGCCTCT CTGTCTATAC AGAGAAAGAG 
AAAGACCCCA AGTATTGGAG AGACCAAGCT CAACAGACCC TGAAATACGC CCTCAGGCTT 
CAGAATCTCA ACACCAACGT GGCAAAGAAT GTCATCATGT TCCTGGGAGA TGGGATGGGT 
GTCTCCACGG TGACGGCCAC TCGCATCCTC AAGGGTCAGC TCCACCACAA CCCCGGGGAG 
GAGACCAGGC TGGAGATGGA CAAGTTCCCT TACGTGGCCC TCTCCAAGAC ATACAACACC 
AACGCTCAGG TCCCTGACAG CGCAGGCACT GCCACCGCCT ACTTGTGTGG AGTGAAGGCC 
AACGAGGGCA CGGTGGGCGT GAGTGCCGCG ACCCAGCGCA CCCAGTGCAA CACGACTCAG 
GGAAACGAGG TCACCTCCAT CCTGCGCTGG GCCAAGGACG CTGGGAAATC TGTGGGCATA 
GTGACCACCA CGAGGGTGAA CCACGCCACC CCCAGTGCCG CCTACGCCCA CTCCGCTGAC 
CGGGACTGGT ACTCGGACAA CGAGATGCCC CCGGAGGCCC TAAGCCAGGG CTGCAAGGAC 
ATTGCCTACC AGCTCATGCA CAACGTCAAG GACATCGAGG TGATCATGGG GGGCGGCCGG 
AAGTACATGT TCCCCAAGAA TAGAACAGAT GTGGAGTATG AGATGGACGA GAAGTCCAGG 
GGCACGAGGC TGGATGGCCT GAACCTCATC GACATCTGGA AGAACTTCAA ACCGAGACAC 
AAGCACTCTC ACTACGTCTG GAACCGCACG GAACTCCTGG CCCTCGACCC CTACACCGTG 
GACTACCTCT TGGGTCTCTT TGAGCCGGGG GACATGCAGT ACGAGCTGAA CAGGAACAAC 
GTGACTGACC CGTCACTCTC CGAGATGGTG GAAATAGCCA TCAAGATTCT GAGCAAGAAC 
CCCAGAGGCT TCTTCTTGCT GGTGGAAGGA GGCAGGATTG ACCACGGGCA TCACGAGGGC 
AAGGCCAAGC AGGCGCTGCA CGAGGCAGTG GAGATGGACC GGGCAATTGG GAAGGCAGGC 
GTCATGACCT CCTTGGAAGA CACGCTGACC GTCGTCACTG CGGACCACTC CCACGTCTTC 
ACCTTTGGCG GGTACACCCC CCGGGGCAAC TCTATCTTTG GTCTGGCCCC CATGGTGAGT 
GACACGGACA AGAAGCCCTT CACGGCCATC CTGTATGGCA ACGGGCCTGG CTACAAGGTG 
GTGGGCGGTG AGCGAGAGAA CGTCTCCATG GTGGACTACG CTCACAACAA CTACCAGGCG 
CAGTCCGCGG TGCCCCTGCG CCACGAGACC CACGGTGGGG AGGACGTAGC AGTCTTCGCC 
AAGGGTCCCA TGGCACACCT GCTGCACGGC GTCCACGAGC AGAACTACAT CCCCCACGTG 
ATGGCTTATG CGGCCTGCAT CGGTGCCAAC CAGGACCACT GTGCCTCAGC CAGCTCGGCA 
GGTGGCCCTT CTTCTCCAGG TCCCCTGCTC CTCCTGCTGG CCCTGCTCCC CGTGGGCATC 
CTATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALPL ( XM_005617214.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617271.1 Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe02910
Clone ID Related Accession (Same CDS sequence)	XM_005617214.3 , XM_005617212.2 , XM_005617213.2 , XM_005617213.3 , XM_005617214.2 , XM_005617212.3
Accession Version	XM_005617214.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617214.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCTC TCTTCCTAGT GCTGGTCATT GGCACCTGCC TTACCAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTATTGGAGA GACCAAGCTC AACAGACCCT GAAATACGCC 
CTCAGGCTTC AGAATCTCAA CACCAACGTG GCAAAGAATG TCATCATGTT CCTGGGAGAT 
GGGATGGGTG TCTCCACGGT GACGGCCACT CGCATCCTCA AGGGTCAGCT CCACCACAAC 
CCCGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCTT ACGTGGCCCT CTCCAAGACA 
TACAACACCA ACGCTCAGGT CCCTGACAGC GCAGGCACTG CCACCGCCTA CTTGTGTGGA 
GTGAAGGCCA ACGAGGGCAC GGTGGGCGTG AGTGCCGCGA CCCAGCGCAC CCAGTGCAAC 
ACGACTCAGG GAAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT 
GTGGGCATAG TGACCACCAC GAGGGTGAAC CACGCCACCC CCAGTGCCGC CTACGCCCAC 
TCCGCTGACC GGGACTGGTA CTCGGACAAC GAGATGCCCC CGGAGGCCCT AAGCCAGGGC 
TGCAAGGACA TTGCCTACCA GCTCATGCAC AACGTCAAGG ACATCGAGGT GATCATGGGG 
GGCGGCCGGA AGTACATGTT CCCCAAGAAT AGAACAGATG TGGAGTATGA GATGGACGAG 
AAGTCCAGGG GCACGAGGCT GGATGGCCTG AACCTCATCG ACATCTGGAA GAACTTCAAA 
CCGAGACACA AGCACTCTCA CTACGTCTGG AACCGCACGG AACTCCTGGC CCTCGACCCC 
TACACCGTGG ACTACCTCTT GGGTCTCTTT GAGCCGGGGG ACATGCAGTA CGAGCTGAAC 
AGGAACAACG TGACTGACCC GTCACTCTCC GAGATGGTGG AAATAGCCAT CAAGATTCTG 
AGCAAGAACC CCAGAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGGCAT 
CACGAGGGCA AGGCCAAGCA GGCGCTGCAC GAGGCAGTGG AGATGGACCG GGCAATTGGG 
AAGGCAGGCG TCATGACCTC CTTGGAAGAC ACGCTGACCG TCGTCACTGC GGACCACTCC 
CACGTCTTCA CCTTTGGCGG GTACACCCCC CGGGGCAACT CTATCTTTGG TCTGGCCCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACGGCCATCC TGTATGGCAA CGGGCCTGGC 
TACAAGGTGG TGGGCGGTGA GCGAGAGAAC GTCTCCATGG TGGACTACGC TCACAACAAC 
TACCAGGCGC AGTCCGCGGT GCCCCTGCGC CACGAGACCC ACGGTGGGGA GGACGTAGCA 
GTCTTCGCCA AGGGTCCCAT GGCACACCTG CTGCACGGCG TCCACGAGCA GAACTACATC 
CCCCACGTGA TGGCTTATGC GGCCTGCATC GGTGCCAACC AGGACCACTG TGCCTCAGCC 
AGCTCGGCAG GTGGCCCTTC TTCTCCAGGT CCCCTGCTCC TCCTGCTGGC CCTGCTCCCC 
GTGGGCATCC TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617271.1
CDS	256..1833
Translation	MISLFLVLVIGTCLTNSLVPEKEKDPKYWRDQAQQTLKYALRLQNLNTNVAKNVIMFLGDGMGVSTVTATRILKGQLHHNPGEETRLEMDKFPYVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATQRTQCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNVKDIEVIMGGGRKYMFPKNRTDVEYEMDEKSRGTRLDGLNLIDIWKNFKPRHKHSHYVWNRTELLALDPYTVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVEIAIKILSKNPRGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGKAGVMTSLEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYAACIGANQDHCASASSAGGPSSPGPLLLLLALLPVGILF

Target ORF information:

RefSeq Version	XM_005617214.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617214.3

ORF Insert Sequence:

ATGATTTCTC TCTTCCTAGT GCTGGTCATT GGCACCTGCC TTACCAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTATTGGAGA GACCAAGCTC AACAGACCCT GAAATACGCC 
CTCAGGCTTC AGAATCTCAA CACCAACGTG GCAAAGAATG TCATCATGTT CCTGGGAGAT 
GGGATGGGTG TCTCCACGGT GACGGCCACT CGCATCCTCA AGGGTCAGCT CCACCACAAC 
CCCGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCTT ACGTGGCCCT CTCCAAGACA 
TACAACACCA ACGCTCAGGT CCCTGACAGC GCAGGCACTG CCACCGCCTA CTTGTGTGGA 
GTGAAGGCCA ACGAGGGCAC GGTGGGCGTG AGTGCCGCGA CCCAGCGCAC CCAGTGCAAC 
ACGACTCAGG GAAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT 
GTGGGCATAG TGACCACCAC GAGGGTGAAC CACGCCACCC CCAGTGCCGC CTACGCCCAC 
TCCGCTGACC GGGACTGGTA CTCGGACAAC GAGATGCCCC CGGAGGCCCT AAGCCAGGGC 
TGCAAGGACA TTGCCTACCA GCTCATGCAC AACGTCAAGG ACATCGAGGT GATCATGGGG 
GGCGGCCGGA AGTACATGTT CCCCAAGAAT AGAACAGATG TGGAGTATGA GATGGACGAG 
AAGTCCAGGG GCACGAGGCT GGATGGCCTG AACCTCATCG ACATCTGGAA GAACTTCAAA 
CCGAGACACA AGCACTCTCA CTACGTCTGG AACCGCACGG AACTCCTGGC CCTCGACCCC 
TACACCGTGG ACTACCTCTT GGGTCTCTTT GAGCCGGGGG ACATGCAGTA CGAGCTGAAC 
AGGAACAACG TGACTGACCC GTCACTCTCC GAGATGGTGG AAATAGCCAT CAAGATTCTG 
AGCAAGAACC CCAGAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGGCAT 
CACGAGGGCA AGGCCAAGCA GGCGCTGCAC GAGGCAGTGG AGATGGACCG GGCAATTGGG 
AAGGCAGGCG TCATGACCTC CTTGGAAGAC ACGCTGACCG TCGTCACTGC GGACCACTCC 
CACGTCTTCA CCTTTGGCGG GTACACCCCC CGGGGCAACT CTATCTTTGG TCTGGCCCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACGGCCATCC TGTATGGCAA CGGGCCTGGC 
TACAAGGTGG TGGGCGGTGA GCGAGAGAAC GTCTCCATGG TGGACTACGC TCACAACAAC 
TACCAGGCGC AGTCCGCGGT GCCCCTGCGC CACGAGACCC ACGGTGGGGA GGACGTAGCA 
GTCTTCGCCA AGGGTCCCAT GGCACACCTG CTGCACGGCG TCCACGAGCA GAACTACATC 
CCCCACGTGA TGGCTTATGC GGCCTGCATC GGTGCCAACC AGGACCACTG TGCCTCAGCC 
AGCTCGGCAG GTGGCCCTTC TTCTCCAGGT CCCCTGCTCC TCCTGCTGGC CCTGCTCCCC 
GTGGGCATCC TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALPL ( XM_005617212.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617269.1 Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe02910
Clone ID Related Accession (Same CDS sequence)	XM_005617214.3 , XM_005617212.2 , XM_005617213.2 , XM_005617213.3 , XM_005617214.2 , XM_005617212.3
Accession Version	XM_005617212.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617212.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCTC TCTTCCTAGT GCTGGTCATT GGCACCTGCC TTACCAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTATTGGAGA GACCAAGCTC AACAGACCCT GAAATACGCC 
CTCAGGCTTC AGAATCTCAA CACCAACGTG GCAAAGAATG TCATCATGTT CCTGGGAGAT 
GGGATGGGTG TCTCCACGGT GACGGCCACT CGCATCCTCA AGGGTCAGCT CCACCACAAC 
CCCGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCTT ACGTGGCCCT CTCCAAGACA 
TACAACACCA ACGCTCAGGT CCCTGACAGC GCAGGCACTG CCACCGCCTA CTTGTGTGGA 
GTGAAGGCCA ACGAGGGCAC GGTGGGCGTG AGTGCCGCGA CCCAGCGCAC CCAGTGCAAC 
ACGACTCAGG GAAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT 
GTGGGCATAG TGACCACCAC GAGGGTGAAC CACGCCACCC CCAGTGCCGC CTACGCCCAC 
TCCGCTGACC GGGACTGGTA CTCGGACAAC GAGATGCCCC CGGAGGCCCT AAGCCAGGGC 
TGCAAGGACA TTGCCTACCA GCTCATGCAC AACGTCAAGG ACATCGAGGT GATCATGGGG 
GGCGGCCGGA AGTACATGTT CCCCAAGAAT AGAACAGATG TGGAGTATGA GATGGACGAG 
AAGTCCAGGG GCACGAGGCT GGATGGCCTG AACCTCATCG ACATCTGGAA GAACTTCAAA 
CCGAGACACA AGCACTCTCA CTACGTCTGG AACCGCACGG AACTCCTGGC CCTCGACCCC 
TACACCGTGG ACTACCTCTT GGGTCTCTTT GAGCCGGGGG ACATGCAGTA CGAGCTGAAC 
AGGAACAACG TGACTGACCC GTCACTCTCC GAGATGGTGG AAATAGCCAT CAAGATTCTG 
AGCAAGAACC CCAGAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGGCAT 
CACGAGGGCA AGGCCAAGCA GGCGCTGCAC GAGGCAGTGG AGATGGACCG GGCAATTGGG 
AAGGCAGGCG TCATGACCTC CTTGGAAGAC ACGCTGACCG TCGTCACTGC GGACCACTCC 
CACGTCTTCA CCTTTGGCGG GTACACCCCC CGGGGCAACT CTATCTTTGG TCTGGCCCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACGGCCATCC TGTATGGCAA CGGGCCTGGC 
TACAAGGTGG TGGGCGGTGA GCGAGAGAAC GTCTCCATGG TGGACTACGC TCACAACAAC 
TACCAGGCGC AGTCCGCGGT GCCCCTGCGC CACGAGACCC ACGGTGGGGA GGACGTAGCA 
GTCTTCGCCA AGGGTCCCAT GGCACACCTG CTGCACGGCG TCCACGAGCA GAACTACATC 
CCCCACGTGA TGGCTTATGC GGCCTGCATC GGTGCCAACC AGGACCACTG TGCCTCAGCC 
AGCTCGGCAG GTGGCCCTTC TTCTCCAGGT CCCCTGCTCC TCCTGCTGGC CCTGCTCCCC 
GTGGGCATCC TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617269.1
CDS	219..1796
Translation	MISLFLVLVIGTCLTNSLVPEKEKDPKYWRDQAQQTLKYALRLQNLNTNVAKNVIMFLGDGMGVSTVTATRILKGQLHHNPGEETRLEMDKFPYVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATQRTQCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNVKDIEVIMGGGRKYMFPKNRTDVEYEMDEKSRGTRLDGLNLIDIWKNFKPRHKHSHYVWNRTELLALDPYTVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVEIAIKILSKNPRGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGKAGVMTSLEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYAACIGANQDHCASASSAGGPSSPGPLLLLLALLPVGILF

Target ORF information:

RefSeq Version	XM_005617212.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617212.2

ORF Insert Sequence:

ATGATTTCTC TCTTCCTAGT GCTGGTCATT GGCACCTGCC TTACCAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTATTGGAGA GACCAAGCTC AACAGACCCT GAAATACGCC 
CTCAGGCTTC AGAATCTCAA CACCAACGTG GCAAAGAATG TCATCATGTT CCTGGGAGAT 
GGGATGGGTG TCTCCACGGT GACGGCCACT CGCATCCTCA AGGGTCAGCT CCACCACAAC 
CCCGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCTT ACGTGGCCCT CTCCAAGACA 
TACAACACCA ACGCTCAGGT CCCTGACAGC GCAGGCACTG CCACCGCCTA CTTGTGTGGA 
GTGAAGGCCA ACGAGGGCAC GGTGGGCGTG AGTGCCGCGA CCCAGCGCAC CCAGTGCAAC 
ACGACTCAGG GAAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT 
GTGGGCATAG TGACCACCAC GAGGGTGAAC CACGCCACCC CCAGTGCCGC CTACGCCCAC 
TCCGCTGACC GGGACTGGTA CTCGGACAAC GAGATGCCCC CGGAGGCCCT AAGCCAGGGC 
TGCAAGGACA TTGCCTACCA GCTCATGCAC AACGTCAAGG ACATCGAGGT GATCATGGGG 
GGCGGCCGGA AGTACATGTT CCCCAAGAAT AGAACAGATG TGGAGTATGA GATGGACGAG 
AAGTCCAGGG GCACGAGGCT GGATGGCCTG AACCTCATCG ACATCTGGAA GAACTTCAAA 
CCGAGACACA AGCACTCTCA CTACGTCTGG AACCGCACGG AACTCCTGGC CCTCGACCCC 
TACACCGTGG ACTACCTCTT GGGTCTCTTT GAGCCGGGGG ACATGCAGTA CGAGCTGAAC 
AGGAACAACG TGACTGACCC GTCACTCTCC GAGATGGTGG AAATAGCCAT CAAGATTCTG 
AGCAAGAACC CCAGAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGGCAT 
CACGAGGGCA AGGCCAAGCA GGCGCTGCAC GAGGCAGTGG AGATGGACCG GGCAATTGGG 
AAGGCAGGCG TCATGACCTC CTTGGAAGAC ACGCTGACCG TCGTCACTGC GGACCACTCC 
CACGTCTTCA CCTTTGGCGG GTACACCCCC CGGGGCAACT CTATCTTTGG TCTGGCCCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACGGCCATCC TGTATGGCAA CGGGCCTGGC 
TACAAGGTGG TGGGCGGTGA GCGAGAGAAC GTCTCCATGG TGGACTACGC TCACAACAAC 
TACCAGGCGC AGTCCGCGGT GCCCCTGCGC CACGAGACCC ACGGTGGGGA GGACGTAGCA 
GTCTTCGCCA AGGGTCCCAT GGCACACCTG CTGCACGGCG TCCACGAGCA GAACTACATC 
CCCCACGTGA TGGCTTATGC GGCCTGCATC GGTGCCAACC AGGACCACTG TGCCTCAGCC 
AGCTCGGCAG GTGGCCCTTC TTCTCCAGGT CCCCTGCTCC TCCTGCTGGC CCTGCTCCCC 
GTGGGCATCC TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALPL ( XM_005617213.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617270.1 Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe02910
Clone ID Related Accession (Same CDS sequence)	XM_005617214.3 , XM_005617212.2 , XM_005617213.2 , XM_005617213.3 , XM_005617214.2 , XM_005617212.3
Accession Version	XM_005617213.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCTC TCTTCCTAGT GCTGGTCATT GGCACCTGCC TTACCAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTATTGGAGA GACCAAGCTC AACAGACCCT GAAATACGCC 
CTCAGGCTTC AGAATCTCAA CACCAACGTG GCAAAGAATG TCATCATGTT CCTGGGAGAT 
GGGATGGGTG TCTCCACGGT GACGGCCACT CGCATCCTCA AGGGTCAGCT CCACCACAAC 
CCCGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCTT ACGTGGCCCT CTCCAAGACA 
TACAACACCA ACGCTCAGGT CCCTGACAGC GCAGGCACTG CCACCGCCTA CTTGTGTGGA 
GTGAAGGCCA ACGAGGGCAC GGTGGGCGTG AGTGCCGCGA CCCAGCGCAC CCAGTGCAAC 
ACGACTCAGG GAAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT 
GTGGGCATAG TGACCACCAC GAGGGTGAAC CACGCCACCC CCAGTGCCGC CTACGCCCAC 
TCCGCTGACC GGGACTGGTA CTCGGACAAC GAGATGCCCC CGGAGGCCCT AAGCCAGGGC 
TGCAAGGACA TTGCCTACCA GCTCATGCAC AACGTCAAGG ACATCGAGGT GATCATGGGG 
GGCGGCCGGA AGTACATGTT CCCCAAGAAT AGAACAGATG TGGAGTATGA GATGGACGAG 
AAGTCCAGGG GCACGAGGCT GGATGGCCTG AACCTCATCG ACATCTGGAA GAACTTCAAA 
CCGAGACACA AGCACTCTCA CTACGTCTGG AACCGCACGG AACTCCTGGC CCTCGACCCC 
TACACCGTGG ACTACCTCTT GGGTCTCTTT GAGCCGGGGG ACATGCAGTA CGAGCTGAAC 
AGGAACAACG TGACTGACCC GTCACTCTCC GAGATGGTGG AAATAGCCAT CAAGATTCTG 
AGCAAGAACC CCAGAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGGCAT 
CACGAGGGCA AGGCCAAGCA GGCGCTGCAC GAGGCAGTGG AGATGGACCG GGCAATTGGG 
AAGGCAGGCG TCATGACCTC CTTGGAAGAC ACGCTGACCG TCGTCACTGC GGACCACTCC 
CACGTCTTCA CCTTTGGCGG GTACACCCCC CGGGGCAACT CTATCTTTGG TCTGGCCCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACGGCCATCC TGTATGGCAA CGGGCCTGGC 
TACAAGGTGG TGGGCGGTGA GCGAGAGAAC GTCTCCATGG TGGACTACGC TCACAACAAC 
TACCAGGCGC AGTCCGCGGT GCCCCTGCGC CACGAGACCC ACGGTGGGGA GGACGTAGCA 
GTCTTCGCCA AGGGTCCCAT GGCACACCTG CTGCACGGCG TCCACGAGCA GAACTACATC 
CCCCACGTGA TGGCTTATGC GGCCTGCATC GGTGCCAACC AGGACCACTG TGCCTCAGCC 
AGCTCGGCAG GTGGCCCTTC TTCTCCAGGT CCCCTGCTCC TCCTGCTGGC CCTGCTCCCC 
GTGGGCATCC TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617270.1
CDS	170..1747
Translation	MISLFLVLVIGTCLTNSLVPEKEKDPKYWRDQAQQTLKYALRLQNLNTNVAKNVIMFLGDGMGVSTVTATRILKGQLHHNPGEETRLEMDKFPYVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATQRTQCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNVKDIEVIMGGGRKYMFPKNRTDVEYEMDEKSRGTRLDGLNLIDIWKNFKPRHKHSHYVWNRTELLALDPYTVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVEIAIKILSKNPRGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGKAGVMTSLEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYAACIGANQDHCASASSAGGPSSPGPLLLLLALLPVGILF

Target ORF information:

RefSeq Version	XM_005617213.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617213.2

ORF Insert Sequence:

ATGATTTCTC TCTTCCTAGT GCTGGTCATT GGCACCTGCC TTACCAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTATTGGAGA GACCAAGCTC AACAGACCCT GAAATACGCC 
CTCAGGCTTC AGAATCTCAA CACCAACGTG GCAAAGAATG TCATCATGTT CCTGGGAGAT 
GGGATGGGTG TCTCCACGGT GACGGCCACT CGCATCCTCA AGGGTCAGCT CCACCACAAC 
CCCGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCTT ACGTGGCCCT CTCCAAGACA 
TACAACACCA ACGCTCAGGT CCCTGACAGC GCAGGCACTG CCACCGCCTA CTTGTGTGGA 
GTGAAGGCCA ACGAGGGCAC GGTGGGCGTG AGTGCCGCGA CCCAGCGCAC CCAGTGCAAC 
ACGACTCAGG GAAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT 
GTGGGCATAG TGACCACCAC GAGGGTGAAC CACGCCACCC CCAGTGCCGC CTACGCCCAC 
TCCGCTGACC GGGACTGGTA CTCGGACAAC GAGATGCCCC CGGAGGCCCT AAGCCAGGGC 
TGCAAGGACA TTGCCTACCA GCTCATGCAC AACGTCAAGG ACATCGAGGT GATCATGGGG 
GGCGGCCGGA AGTACATGTT CCCCAAGAAT AGAACAGATG TGGAGTATGA GATGGACGAG 
AAGTCCAGGG GCACGAGGCT GGATGGCCTG AACCTCATCG ACATCTGGAA GAACTTCAAA 
CCGAGACACA AGCACTCTCA CTACGTCTGG AACCGCACGG AACTCCTGGC CCTCGACCCC 
TACACCGTGG ACTACCTCTT GGGTCTCTTT GAGCCGGGGG ACATGCAGTA CGAGCTGAAC 
AGGAACAACG TGACTGACCC GTCACTCTCC GAGATGGTGG AAATAGCCAT CAAGATTCTG 
AGCAAGAACC CCAGAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGGCAT 
CACGAGGGCA AGGCCAAGCA GGCGCTGCAC GAGGCAGTGG AGATGGACCG GGCAATTGGG 
AAGGCAGGCG TCATGACCTC CTTGGAAGAC ACGCTGACCG TCGTCACTGC GGACCACTCC 
CACGTCTTCA CCTTTGGCGG GTACACCCCC CGGGGCAACT CTATCTTTGG TCTGGCCCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACGGCCATCC TGTATGGCAA CGGGCCTGGC 
TACAAGGTGG TGGGCGGTGA GCGAGAGAAC GTCTCCATGG TGGACTACGC TCACAACAAC 
TACCAGGCGC AGTCCGCGGT GCCCCTGCGC CACGAGACCC ACGGTGGGGA GGACGTAGCA 
GTCTTCGCCA AGGGTCCCAT GGCACACCTG CTGCACGGCG TCCACGAGCA GAACTACATC 
CCCCACGTGA TGGCTTATGC GGCCTGCATC GGTGCCAACC AGGACCACTG TGCCTCAGCC 
AGCTCGGCAG GTGGCCCTTC TTCTCCAGGT CCCCTGCTCC TCCTGCTGGC CCTGCTCCCC 
GTGGGCATCC TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALPL ( XM_005617213.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617270.1 Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe02910
Clone ID Related Accession (Same CDS sequence)	XM_005617214.3 , XM_005617212.2 , XM_005617213.2 , XM_005617213.3 , XM_005617214.2 , XM_005617212.3
Accession Version	XM_005617213.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617213.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCTC TCTTCCTAGT GCTGGTCATT GGCACCTGCC TTACCAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTATTGGAGA GACCAAGCTC AACAGACCCT GAAATACGCC 
CTCAGGCTTC AGAATCTCAA CACCAACGTG GCAAAGAATG TCATCATGTT CCTGGGAGAT 
GGGATGGGTG TCTCCACGGT GACGGCCACT CGCATCCTCA AGGGTCAGCT CCACCACAAC 
CCCGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCTT ACGTGGCCCT CTCCAAGACA 
TACAACACCA ACGCTCAGGT CCCTGACAGC GCAGGCACTG CCACCGCCTA CTTGTGTGGA 
GTGAAGGCCA ACGAGGGCAC GGTGGGCGTG AGTGCCGCGA CCCAGCGCAC CCAGTGCAAC 
ACGACTCAGG GAAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT 
GTGGGCATAG TGACCACCAC GAGGGTGAAC CACGCCACCC CCAGTGCCGC CTACGCCCAC 
TCCGCTGACC GGGACTGGTA CTCGGACAAC GAGATGCCCC CGGAGGCCCT AAGCCAGGGC 
TGCAAGGACA TTGCCTACCA GCTCATGCAC AACGTCAAGG ACATCGAGGT GATCATGGGG 
GGCGGCCGGA AGTACATGTT CCCCAAGAAT AGAACAGATG TGGAGTATGA GATGGACGAG 
AAGTCCAGGG GCACGAGGCT GGATGGCCTG AACCTCATCG ACATCTGGAA GAACTTCAAA 
CCGAGACACA AGCACTCTCA CTACGTCTGG AACCGCACGG AACTCCTGGC CCTCGACCCC 
TACACCGTGG ACTACCTCTT GGGTCTCTTT GAGCCGGGGG ACATGCAGTA CGAGCTGAAC 
AGGAACAACG TGACTGACCC GTCACTCTCC GAGATGGTGG AAATAGCCAT CAAGATTCTG 
AGCAAGAACC CCAGAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGGCAT 
CACGAGGGCA AGGCCAAGCA GGCGCTGCAC GAGGCAGTGG AGATGGACCG GGCAATTGGG 
AAGGCAGGCG TCATGACCTC CTTGGAAGAC ACGCTGACCG TCGTCACTGC GGACCACTCC 
CACGTCTTCA CCTTTGGCGG GTACACCCCC CGGGGCAACT CTATCTTTGG TCTGGCCCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACGGCCATCC TGTATGGCAA CGGGCCTGGC 
TACAAGGTGG TGGGCGGTGA GCGAGAGAAC GTCTCCATGG TGGACTACGC TCACAACAAC 
TACCAGGCGC AGTCCGCGGT GCCCCTGCGC CACGAGACCC ACGGTGGGGA GGACGTAGCA 
GTCTTCGCCA AGGGTCCCAT GGCACACCTG CTGCACGGCG TCCACGAGCA GAACTACATC 
CCCCACGTGA TGGCTTATGC GGCCTGCATC GGTGCCAACC AGGACCACTG TGCCTCAGCC 
AGCTCGGCAG GTGGCCCTTC TTCTCCAGGT CCCCTGCTCC TCCTGCTGGC CCTGCTCCCC 
GTGGGCATCC TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617270.1
CDS	231..1808
Translation	MISLFLVLVIGTCLTNSLVPEKEKDPKYWRDQAQQTLKYALRLQNLNTNVAKNVIMFLGDGMGVSTVTATRILKGQLHHNPGEETRLEMDKFPYVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATQRTQCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNVKDIEVIMGGGRKYMFPKNRTDVEYEMDEKSRGTRLDGLNLIDIWKNFKPRHKHSHYVWNRTELLALDPYTVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVEIAIKILSKNPRGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGKAGVMTSLEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYAACIGANQDHCASASSAGGPSSPGPLLLLLALLPVGILF

Target ORF information:

RefSeq Version	XM_005617213.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617213.3

ORF Insert Sequence:

ATGATTTCTC TCTTCCTAGT GCTGGTCATT GGCACCTGCC TTACCAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTATTGGAGA GACCAAGCTC AACAGACCCT GAAATACGCC 
CTCAGGCTTC AGAATCTCAA CACCAACGTG GCAAAGAATG TCATCATGTT CCTGGGAGAT 
GGGATGGGTG TCTCCACGGT GACGGCCACT CGCATCCTCA AGGGTCAGCT CCACCACAAC 
CCCGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCTT ACGTGGCCCT CTCCAAGACA 
TACAACACCA ACGCTCAGGT CCCTGACAGC GCAGGCACTG CCACCGCCTA CTTGTGTGGA 
GTGAAGGCCA ACGAGGGCAC GGTGGGCGTG AGTGCCGCGA CCCAGCGCAC CCAGTGCAAC 
ACGACTCAGG GAAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT 
GTGGGCATAG TGACCACCAC GAGGGTGAAC CACGCCACCC CCAGTGCCGC CTACGCCCAC 
TCCGCTGACC GGGACTGGTA CTCGGACAAC GAGATGCCCC CGGAGGCCCT AAGCCAGGGC 
TGCAAGGACA TTGCCTACCA GCTCATGCAC AACGTCAAGG ACATCGAGGT GATCATGGGG 
GGCGGCCGGA AGTACATGTT CCCCAAGAAT AGAACAGATG TGGAGTATGA GATGGACGAG 
AAGTCCAGGG GCACGAGGCT GGATGGCCTG AACCTCATCG ACATCTGGAA GAACTTCAAA 
CCGAGACACA AGCACTCTCA CTACGTCTGG AACCGCACGG AACTCCTGGC CCTCGACCCC 
TACACCGTGG ACTACCTCTT GGGTCTCTTT GAGCCGGGGG ACATGCAGTA CGAGCTGAAC 
AGGAACAACG TGACTGACCC GTCACTCTCC GAGATGGTGG AAATAGCCAT CAAGATTCTG 
AGCAAGAACC CCAGAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGGCAT 
CACGAGGGCA AGGCCAAGCA GGCGCTGCAC GAGGCAGTGG AGATGGACCG GGCAATTGGG 
AAGGCAGGCG TCATGACCTC CTTGGAAGAC ACGCTGACCG TCGTCACTGC GGACCACTCC 
CACGTCTTCA CCTTTGGCGG GTACACCCCC CGGGGCAACT CTATCTTTGG TCTGGCCCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACGGCCATCC TGTATGGCAA CGGGCCTGGC 
TACAAGGTGG TGGGCGGTGA GCGAGAGAAC GTCTCCATGG TGGACTACGC TCACAACAAC 
TACCAGGCGC AGTCCGCGGT GCCCCTGCGC CACGAGACCC ACGGTGGGGA GGACGTAGCA 
GTCTTCGCCA AGGGTCCCAT GGCACACCTG CTGCACGGCG TCCACGAGCA GAACTACATC 
CCCCACGTGA TGGCTTATGC GGCCTGCATC GGTGCCAACC AGGACCACTG TGCCTCAGCC 
AGCTCGGCAG GTGGCCCTTC TTCTCCAGGT CCCCTGCTCC TCCTGCTGGC CCTGCTCCCC 
GTGGGCATCC TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALPL ( XM_005617214.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617271.1 Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe02910
Clone ID Related Accession (Same CDS sequence)	XM_005617214.3 , XM_005617212.2 , XM_005617213.2 , XM_005617213.3 , XM_005617214.2 , XM_005617212.3
Accession Version	XM_005617214.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617214.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCTC TCTTCCTAGT GCTGGTCATT GGCACCTGCC TTACCAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTATTGGAGA GACCAAGCTC AACAGACCCT GAAATACGCC 
CTCAGGCTTC AGAATCTCAA CACCAACGTG GCAAAGAATG TCATCATGTT CCTGGGAGAT 
GGGATGGGTG TCTCCACGGT GACGGCCACT CGCATCCTCA AGGGTCAGCT CCACCACAAC 
CCCGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCTT ACGTGGCCCT CTCCAAGACA 
TACAACACCA ACGCTCAGGT CCCTGACAGC GCAGGCACTG CCACCGCCTA CTTGTGTGGA 
GTGAAGGCCA ACGAGGGCAC GGTGGGCGTG AGTGCCGCGA CCCAGCGCAC CCAGTGCAAC 
ACGACTCAGG GAAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT 
GTGGGCATAG TGACCACCAC GAGGGTGAAC CACGCCACCC CCAGTGCCGC CTACGCCCAC 
TCCGCTGACC GGGACTGGTA CTCGGACAAC GAGATGCCCC CGGAGGCCCT AAGCCAGGGC 
TGCAAGGACA TTGCCTACCA GCTCATGCAC AACGTCAAGG ACATCGAGGT GATCATGGGG 
GGCGGCCGGA AGTACATGTT CCCCAAGAAT AGAACAGATG TGGAGTATGA GATGGACGAG 
AAGTCCAGGG GCACGAGGCT GGATGGCCTG AACCTCATCG ACATCTGGAA GAACTTCAAA 
CCGAGACACA AGCACTCTCA CTACGTCTGG AACCGCACGG AACTCCTGGC CCTCGACCCC 
TACACCGTGG ACTACCTCTT GGGTCTCTTT GAGCCGGGGG ACATGCAGTA CGAGCTGAAC 
AGGAACAACG TGACTGACCC GTCACTCTCC GAGATGGTGG AAATAGCCAT CAAGATTCTG 
AGCAAGAACC CCAGAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGGCAT 
CACGAGGGCA AGGCCAAGCA GGCGCTGCAC GAGGCAGTGG AGATGGACCG GGCAATTGGG 
AAGGCAGGCG TCATGACCTC CTTGGAAGAC ACGCTGACCG TCGTCACTGC GGACCACTCC 
CACGTCTTCA CCTTTGGCGG GTACACCCCC CGGGGCAACT CTATCTTTGG TCTGGCCCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACGGCCATCC TGTATGGCAA CGGGCCTGGC 
TACAAGGTGG TGGGCGGTGA GCGAGAGAAC GTCTCCATGG TGGACTACGC TCACAACAAC 
TACCAGGCGC AGTCCGCGGT GCCCCTGCGC CACGAGACCC ACGGTGGGGA GGACGTAGCA 
GTCTTCGCCA AGGGTCCCAT GGCACACCTG CTGCACGGCG TCCACGAGCA GAACTACATC 
CCCCACGTGA TGGCTTATGC GGCCTGCATC GGTGCCAACC AGGACCACTG TGCCTCAGCC 
AGCTCGGCAG GTGGCCCTTC TTCTCCAGGT CCCCTGCTCC TCCTGCTGGC CCTGCTCCCC 
GTGGGCATCC TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617271.1
CDS	203..1780
Translation	MISLFLVLVIGTCLTNSLVPEKEKDPKYWRDQAQQTLKYALRLQNLNTNVAKNVIMFLGDGMGVSTVTATRILKGQLHHNPGEETRLEMDKFPYVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATQRTQCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNVKDIEVIMGGGRKYMFPKNRTDVEYEMDEKSRGTRLDGLNLIDIWKNFKPRHKHSHYVWNRTELLALDPYTVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVEIAIKILSKNPRGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGKAGVMTSLEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYAACIGANQDHCASASSAGGPSSPGPLLLLLALLPVGILF

Target ORF information:

RefSeq Version	XM_005617214.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617214.2

ORF Insert Sequence:

ATGATTTCTC TCTTCCTAGT GCTGGTCATT GGCACCTGCC TTACCAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTATTGGAGA GACCAAGCTC AACAGACCCT GAAATACGCC 
CTCAGGCTTC AGAATCTCAA CACCAACGTG GCAAAGAATG TCATCATGTT CCTGGGAGAT 
GGGATGGGTG TCTCCACGGT GACGGCCACT CGCATCCTCA AGGGTCAGCT CCACCACAAC 
CCCGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCTT ACGTGGCCCT CTCCAAGACA 
TACAACACCA ACGCTCAGGT CCCTGACAGC GCAGGCACTG CCACCGCCTA CTTGTGTGGA 
GTGAAGGCCA ACGAGGGCAC GGTGGGCGTG AGTGCCGCGA CCCAGCGCAC CCAGTGCAAC 
ACGACTCAGG GAAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT 
GTGGGCATAG TGACCACCAC GAGGGTGAAC CACGCCACCC CCAGTGCCGC CTACGCCCAC 
TCCGCTGACC GGGACTGGTA CTCGGACAAC GAGATGCCCC CGGAGGCCCT AAGCCAGGGC 
TGCAAGGACA TTGCCTACCA GCTCATGCAC AACGTCAAGG ACATCGAGGT GATCATGGGG 
GGCGGCCGGA AGTACATGTT CCCCAAGAAT AGAACAGATG TGGAGTATGA GATGGACGAG 
AAGTCCAGGG GCACGAGGCT GGATGGCCTG AACCTCATCG ACATCTGGAA GAACTTCAAA 
CCGAGACACA AGCACTCTCA CTACGTCTGG AACCGCACGG AACTCCTGGC CCTCGACCCC 
TACACCGTGG ACTACCTCTT GGGTCTCTTT GAGCCGGGGG ACATGCAGTA CGAGCTGAAC 
AGGAACAACG TGACTGACCC GTCACTCTCC GAGATGGTGG AAATAGCCAT CAAGATTCTG 
AGCAAGAACC CCAGAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGGCAT 
CACGAGGGCA AGGCCAAGCA GGCGCTGCAC GAGGCAGTGG AGATGGACCG GGCAATTGGG 
AAGGCAGGCG TCATGACCTC CTTGGAAGAC ACGCTGACCG TCGTCACTGC GGACCACTCC 
CACGTCTTCA CCTTTGGCGG GTACACCCCC CGGGGCAACT CTATCTTTGG TCTGGCCCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACGGCCATCC TGTATGGCAA CGGGCCTGGC 
TACAAGGTGG TGGGCGGTGA GCGAGAGAAC GTCTCCATGG TGGACTACGC TCACAACAAC 
TACCAGGCGC AGTCCGCGGT GCCCCTGCGC CACGAGACCC ACGGTGGGGA GGACGTAGCA 
GTCTTCGCCA AGGGTCCCAT GGCACACCTG CTGCACGGCG TCCACGAGCA GAACTACATC 
CCCCACGTGA TGGCTTATGC GGCCTGCATC GGTGCCAACC AGGACCACTG TGCCTCAGCC 
AGCTCGGCAG GTGGCCCTTC TTCTCCAGGT CCCCTGCTCC TCCTGCTGGC CCTGCTCCCC 
GTGGGCATCC TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALPL ( XM_005617212.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617269.1 Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X3, mRNA.

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CloneID	OCe02910
Clone ID Related Accession (Same CDS sequence)	XM_005617214.3 , XM_005617212.2 , XM_005617213.2 , XM_005617213.3 , XM_005617214.2 , XM_005617212.3
Accession Version	XM_005617212.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617212.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCTC TCTTCCTAGT GCTGGTCATT GGCACCTGCC TTACCAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTATTGGAGA GACCAAGCTC AACAGACCCT GAAATACGCC 
CTCAGGCTTC AGAATCTCAA CACCAACGTG GCAAAGAATG TCATCATGTT CCTGGGAGAT 
GGGATGGGTG TCTCCACGGT GACGGCCACT CGCATCCTCA AGGGTCAGCT CCACCACAAC 
CCCGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCTT ACGTGGCCCT CTCCAAGACA 
TACAACACCA ACGCTCAGGT CCCTGACAGC GCAGGCACTG CCACCGCCTA CTTGTGTGGA 
GTGAAGGCCA ACGAGGGCAC GGTGGGCGTG AGTGCCGCGA CCCAGCGCAC CCAGTGCAAC 
ACGACTCAGG GAAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT 
GTGGGCATAG TGACCACCAC GAGGGTGAAC CACGCCACCC CCAGTGCCGC CTACGCCCAC 
TCCGCTGACC GGGACTGGTA CTCGGACAAC GAGATGCCCC CGGAGGCCCT AAGCCAGGGC 
TGCAAGGACA TTGCCTACCA GCTCATGCAC AACGTCAAGG ACATCGAGGT GATCATGGGG 
GGCGGCCGGA AGTACATGTT CCCCAAGAAT AGAACAGATG TGGAGTATGA GATGGACGAG 
AAGTCCAGGG GCACGAGGCT GGATGGCCTG AACCTCATCG ACATCTGGAA GAACTTCAAA 
CCGAGACACA AGCACTCTCA CTACGTCTGG AACCGCACGG AACTCCTGGC CCTCGACCCC 
TACACCGTGG ACTACCTCTT GGGTCTCTTT GAGCCGGGGG ACATGCAGTA CGAGCTGAAC 
AGGAACAACG TGACTGACCC GTCACTCTCC GAGATGGTGG AAATAGCCAT CAAGATTCTG 
AGCAAGAACC CCAGAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGGCAT 
CACGAGGGCA AGGCCAAGCA GGCGCTGCAC GAGGCAGTGG AGATGGACCG GGCAATTGGG 
AAGGCAGGCG TCATGACCTC CTTGGAAGAC ACGCTGACCG TCGTCACTGC GGACCACTCC 
CACGTCTTCA CCTTTGGCGG GTACACCCCC CGGGGCAACT CTATCTTTGG TCTGGCCCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACGGCCATCC TGTATGGCAA CGGGCCTGGC 
TACAAGGTGG TGGGCGGTGA GCGAGAGAAC GTCTCCATGG TGGACTACGC TCACAACAAC 
TACCAGGCGC AGTCCGCGGT GCCCCTGCGC CACGAGACCC ACGGTGGGGA GGACGTAGCA 
GTCTTCGCCA AGGGTCCCAT GGCACACCTG CTGCACGGCG TCCACGAGCA GAACTACATC 
CCCCACGTGA TGGCTTATGC GGCCTGCATC GGTGCCAACC AGGACCACTG TGCCTCAGCC 
AGCTCGGCAG GTGGCCCTTC TTCTCCAGGT CCCCTGCTCC TCCTGCTGGC CCTGCTCCCC 
GTGGGCATCC TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617269.1
CDS	215..1792
Translation	MISLFLVLVIGTCLTNSLVPEKEKDPKYWRDQAQQTLKYALRLQNLNTNVAKNVIMFLGDGMGVSTVTATRILKGQLHHNPGEETRLEMDKFPYVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATQRTQCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNVKDIEVIMGGGRKYMFPKNRTDVEYEMDEKSRGTRLDGLNLIDIWKNFKPRHKHSHYVWNRTELLALDPYTVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVEIAIKILSKNPRGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGKAGVMTSLEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYAACIGANQDHCASASSAGGPSSPGPLLLLLALLPVGILF

Target ORF information:

RefSeq Version	XM_005617212.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617212.3

ORF Insert Sequence:

ATGATTTCTC TCTTCCTAGT GCTGGTCATT GGCACCTGCC TTACCAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTATTGGAGA GACCAAGCTC AACAGACCCT GAAATACGCC 
CTCAGGCTTC AGAATCTCAA CACCAACGTG GCAAAGAATG TCATCATGTT CCTGGGAGAT 
GGGATGGGTG TCTCCACGGT GACGGCCACT CGCATCCTCA AGGGTCAGCT CCACCACAAC 
CCCGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCTT ACGTGGCCCT CTCCAAGACA 
TACAACACCA ACGCTCAGGT CCCTGACAGC GCAGGCACTG CCACCGCCTA CTTGTGTGGA 
GTGAAGGCCA ACGAGGGCAC GGTGGGCGTG AGTGCCGCGA CCCAGCGCAC CCAGTGCAAC 
ACGACTCAGG GAAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT 
GTGGGCATAG TGACCACCAC GAGGGTGAAC CACGCCACCC CCAGTGCCGC CTACGCCCAC 
TCCGCTGACC GGGACTGGTA CTCGGACAAC GAGATGCCCC CGGAGGCCCT AAGCCAGGGC 
TGCAAGGACA TTGCCTACCA GCTCATGCAC AACGTCAAGG ACATCGAGGT GATCATGGGG 
GGCGGCCGGA AGTACATGTT CCCCAAGAAT AGAACAGATG TGGAGTATGA GATGGACGAG 
AAGTCCAGGG GCACGAGGCT GGATGGCCTG AACCTCATCG ACATCTGGAA GAACTTCAAA 
CCGAGACACA AGCACTCTCA CTACGTCTGG AACCGCACGG AACTCCTGGC CCTCGACCCC 
TACACCGTGG ACTACCTCTT GGGTCTCTTT GAGCCGGGGG ACATGCAGTA CGAGCTGAAC 
AGGAACAACG TGACTGACCC GTCACTCTCC GAGATGGTGG AAATAGCCAT CAAGATTCTG 
AGCAAGAACC CCAGAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGGCAT 
CACGAGGGCA AGGCCAAGCA GGCGCTGCAC GAGGCAGTGG AGATGGACCG GGCAATTGGG 
AAGGCAGGCG TCATGACCTC CTTGGAAGAC ACGCTGACCG TCGTCACTGC GGACCACTCC 
CACGTCTTCA CCTTTGGCGG GTACACCCCC CGGGGCAACT CTATCTTTGG TCTGGCCCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACGGCCATCC TGTATGGCAA CGGGCCTGGC 
TACAAGGTGG TGGGCGGTGA GCGAGAGAAC GTCTCCATGG TGGACTACGC TCACAACAAC 
TACCAGGCGC AGTCCGCGGT GCCCCTGCGC CACGAGACCC ACGGTGGGGA GGACGTAGCA 
GTCTTCGCCA AGGGTCCCAT GGCACACCTG CTGCACGGCG TCCACGAGCA GAACTACATC 
CCCCACGTGA TGGCTTATGC GGCCTGCATC GGTGCCAACC AGGACCACTG TGCCTCAGCC 
AGCTCGGCAG GTGGCCCTTC TTCTCCAGGT CCCCTGCTCC TCCTGCTGGC CCTGCTCCCC 
GTGGGCATCC TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Association of Endothelin-1 Signaling with Bone Alkaline Phosphatase Expression and Protumorigenic Activities in Canine Osteosarcoma.
Journal of veterinary internal medicine29(6)1584-94(2015 Nov-Dec)
Neumann ZL,Pondenis HC,Masyr A,Byrum ML,Wycislo KL,Fan TM

Association between absolute tumor burden and serum bone-specific alkaline phosphatase in canine appendicular osteosarcoma.
Journal of veterinary internal medicine27(4)955-63(2013 Jul-Aug)
Sternberg RA,Pondenis HC,Yang X,Mitchell MA,O'Brien RT,Garrett LD,Helferich WG,Hoffmann WE,Fan TM

The influence of sex on biochemical markers of bone turnover in dogs.
Research in veterinary science93(2)918-20(2012 Oct)
Belić M,Kušec V,Svetina A,Grizelj J,Robić M,Vrbanac Z,Benić M,Turk R

Wnt/β-catenin expression does not correlate with serum alkaline phosphatase concentration in canine osteosarcoma patients.
PloS one6(10)e26106(2011)
Piskun CM,Muthuswamy A,Huelsmeyer MK,Thompson V,Stein TJ

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ALPL cDNA ORF clone, Canis lupus familiaris(dog)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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