PROB1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PROB1
Entrez Gene ID	100688546
Full Name	proline rich basic protein 1
General protein information	Preferred Names proline rich basic protein 1 Names proline-rich basic protein 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005617255.2	XP_005617312.1	proline-rich basic protein 1
XM_005617255.3	XP_005617312.1	proline-rich basic protein 1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LOC693695	XP_001083163.1
Bos taurus (cattle)	PROB1	XP_003586356.1
Pan troglodytes (chimpanzee)	PROB1	XP_003950689.1
Homo sapiens (human)	PROB1	NP_001155018.1
Mus musculus (house mouse)	Prob1	NP_001257575.1
Rattus norvegicus (Norway rat)	Prob1	XP_001069975.1
Canis lupus familiaris (dog)	PROB1	XP_005617312.1

Gene Ontology

The following PROB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PROB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe02954	XM_005617255.3 Latest version!	Canis lupus familiaris proline rich basic protein 1 (PROB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCe02954	XM_005617255.2	Canis lupus familiaris proline-rich basic protein 1 (PROB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PROB1 ( XM_005617255.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617312.1 Canis lupus familiaris proline-rich basic protein 1 (PROB1), mRNA.

Price & Availability↑

CloneID	OCe02954
Clone ID Related Accession (Same CDS sequence)	XM_005617255.2 , XM_005617255.3
Accession Version	XM_005617255.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	proline-rich basic protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACCG CGCTCGCCCG GCCAGCCCGG CCTGGGCTCC CGGGGCAGCC GCCTGCGGCC 
CCCGCCCGGC GTCAGGACTC CTCTGGCTCG TCAGGCTCCT ACCACACGGC TCCGGGTTCT 
CCAGGGCCCC CGGACGTTGG GCCGGACGCA GAGTGCCGGG CGCATTGGCC CGGGGTGGCC 
CCTGCGCTGG GGGCGGGCGC GCAGCCTCGC CTGTCCGTCA GCGCCCAGAA TAGCCGCCAG 
CAGCCCGGGC CCGGCTCGGG TTTCCCGCGA GGCCGGGGCT CCAGCCCGCG GCCGCCCCAG 
CCGCAGCTGC GCATGCTGCC GTCGGGGGAG ATGGAAGTCA TCTTCGGCGC AGGGGCCCCG 
TTCCGCCGCT CCGACGCGGC GGATAGCGAG GTGCAGCAAC TCACCGCCCG CGCTTTCCGC 
AGCCTCTCTC CGCCCGGGCC CGCGACTCCG GCTCCCGCGA CACCGACGCC CCAGGCCCCC 
GACGGTGGCT CCCGCTGGGC CACTTACCTA GAGGTACGGC CCCGCGGGCC GAGTCCTGGG 
ACCCCAGCGC AGTTCGAGTG TGTGGAGGTG GCGCTGGAGG AGCGTGAGGC TCCCGCCAGG 
TCCCGCACGG TGCCCAAGCG TCAGATCGAG CTGCGCCCCC GGCCCCAGAG TCCGCCGCGG 
GAGGCGGGAG CACCACCGCG CCCCCGGCTG CTCCTGCGCA CTGGCTCCCT GGACGAGTCG 
CTGGGCCGCC TGCAGGAAGC CGCTGGCCTG GTGCAGACAG CACTGGCCAG AAAACTGAGC 
CCTGCAGCCC CTGCCCCAAG CAGCGCCACC TTCGGGCCCA CGGGGCAGCC AGAGTGTGCG 
AGCCGAGAAA CGGCCCGCAG TACCCGAAAG GTCCCGGAGG AGGCCAGGTC TCGGCCACCG 
CGCGTGCACT ATGGTTCAGC ACCCACCAAG GCACCGCGGC CGTGGCCCAG CCTCCGAGAG 
CGCGCGATTC GGCGCGACAA GCCCGCGCCG GGCACCGAGC CGCTGGGTCC GGTTAGTTCC 
AGCATCTTCC TGCAGTCAGG GGAGAAGACT CGGGAGGCAC AAAGTCAGGA ACTTAAGACT 
CGGTCCCCGC GACAGACTCC GGATCGAGCC ATTCTGAGAG CACAGAGTCC GCCTTTCGAG 
TCGAGGGGCC CTCGGGAAGT TCCGAGTAAG GCTGGGAGGC CATGGAGCTC GTCCCCGCTG 
TGGCAGGCTC CAAATGGGAC CGTACGGGGT CCTCACTGCC CGTCCCCCCA GAATCCGCCC 
CCGTGGAATC GGGCTATTCG GAGGGTAAGT AGCCCGTCGT TCCCTGAAGC ATCCCGTGCA 
TGGGGAAATC GGGATCCCGC TGTCGAGGAA ACTGTCAGCA GAAGAAGCCC CTCCCCACCG 
ACTGTTTCCC CGTGGAATCA GGGTGTCGCC AGGACAAGAA GCTCATCCCC TGAAGCTCCT 
TCCCTGTGGG AGGTTCCTCA ACCGGCAGTT GGGAATACCG CAGAGGGCAG TAGGAGCGCG 
TATCCACCGG CCTTGTCCTC ATGGGAGGCT CCAGATCGTG CTAATGGGAC GTGGCATCCA 
TCTCCCCAAG ACACGTGGGG TCCCACACCA CAGGGCTCAT CCATAGTATC TACGTCTAAA 
GCTCTGAATG GGATAGGAAT GGAAGAGCTG GAGCGGCCTA GGCCGCCCAC ACCTGGGACT 
CCGGAACTCA CGGAGGCGCA AGGTCAGTCC TCGCGGGAGG TGCAGGATCT CGCCTTCCGA 
GGCAGTCAGC CGTCGCCAGA AGCGGCCGCG CCTGAGCTAA CCCTCAGTCG CCTTGTGGGC 
CCTGTGGATG CCGATGTGCC CCGGGAAGCC CTGGGCCTTG GAGAGGCCGC CTCGGGACGG 
CCTCGCGTGG CCCTCCCGCG GCCCCGCGAC GTACGCAAGA TGGTGAAGAC CACGTACGCG 
CCAAGCTTCC CCGCGGGAAC CGCAGCTTTG CGGCTGCCTG CGCCCCCTGT GGAACCCGGC 
GCGGAGGAGG GCGGCGCGTC CGGGACCGAA GCGTTGCCCG CGCTGGCGTC GCCAGCCCCA 
GCTCACTACA CTTCGGTTTA CCTCAAAGAC TTTCTTCCGG TCGTGACGCA CCCCTACGAG 
CCCCCAGAGC CGTCCCCTGA CGCGGTGCCC CGGGACGCCC CGCGGCCCAA CGGGGCCCTG 
AGGCGGAGAG CGGAGAACAG CACGGCCAAA CCCTTCGCAC GCACGGAGAT CCGCCTGCCT 
GCGGCCCTGG CCGTGGGCCG CCGGCCCGAG GGAGCGGGGG CAGTTGTGCG CGGTGCTGGC 
GGGGAGAGCC GCGAGGCTGA GGCGCGGCGC CTGGTCCCCG ACGGCGAGGG GCGGACCAGC 
CCCTTAGGCG GCGCGCGCGC GTCACCCCAG CCGCCGCCCA CGGGCCCGGG AGGGGCCCAG 
CCACCCCGAC CACCGTGCCC CCGCTCGCCT CAGGCGCACC CCAGCTCGAG CGCCGGGACA 
GCACCCGAAG TGGAGACGTC TCCTGCAGCT CCCGAGCCGG CGACTGCGAT CCAGTCGTCT 
CTCCTGCGGG AGTCCCAGGC GTCGGCCGGC AGAACCGGCC CGCCCCAACC CCGCGCTGCC 
TCAGCGCCTC CGACGGACCG GCCCCTCGAA GGCCCCCTCC ACGGGGCACG CAGGCCCCCA 
GGGACTGCGT ACCCGGGGAA GGTCCTGGTG GACCCCGAGA GCGGCCGTTG CTACTTTGTG 
GAGGCGCCGC GGCAGCCTCG GCTACGGCTA CTCTTCGACC CCGAAAGCGG GCAATACGTA 
GAGGTGCTGC TGCCGCCATC GCCCCAGGTG CCACCACGCC GCGTCTACAC CCCGCTGGCC 
CTGGGCTCTG GCCTCTACCC GCCCGCCTAT GGGCCTCTCC CGGGCCTCTC AGTGCCACCG 
TCCCTGGCCC CAGCGGGCTT CAGCGGCCCC CAGGTACCCT GGGCCTCCGA CGCGGGGCCC 
CTGGACGGGA TGTACTACCT GCCAGTGAGT GGAACCCCCA GCCCCGCTCC CCCTCTGCTC 
CTCTGTGCTC CGCCCAACAG TTCGAGTTCA GCCCAGCCCG GCAAGGGGTC CTTGTTCTCA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617312.1
CDS	1842..4907
Translation	MLTALARPARPGLPGQPPAAPARRQDSSGSSGSYHTAPGSPGPPDVGPDAECRAHWPGVAPALGAGAQPRLSVSAQNSRQQPGPGSGFPRGRGSSPRPPQPQLRMLPSGEMEVIFGAGAPFRRSDAADSEVQQLTARAFRSLSPPGPATPAPATPTPQAPDGGSRWATYLEVRPRGPSPGTPAQFECVEVALEEREAPARSRTVPKRQIELRPRPQSPPREAGAPPRPRLLLRTGSLDESLGRLQEAAGLVQTALARKLSPAAPAPSSATFGPTGQPECASRETARSTRKVPEEARSRPPRVHYGSAPTKAPRPWPSLRERAIRRDKPAPGTEPLGPVSSSIFLQSGEKTREAQSQELKTRSPRQTPDRAILRAQSPPFESRGPREVPSKAGRPWSSSPLWQAPNGTVRGPHCPSPQNPPPWNRAIRRVSSPSFPEASRAWGNRDPAVEETVSRRSPSPPTVSPWNQGVARTRSSSPEAPSLWEVPQPAVGNTAEGSRSAYPPALSSWEAPDRANGTWHPSPQDTWGPTPQGSSIVSTSKALNGIGMEELERPRPPTPGTPELTEAQGQSSREVQDLAFRGSQPSPEAAAPELTLSRLVGPVDADVPREALGLGEAASGRPRVALPRPRDVRKMVKTTYAPSFPAGTAALRLPAPPVEPGAEEGGASGTEALPALASPAPAHYTSVYLKDFLPVVTHPYEPPEPSPDAVPRDAPRPNGALRRRAENSTAKPFARTEIRLPAALAVGRRPEGAGAVVRGAGGESREAEARRLVPDGEGRTSPLGGARASPQPPPTGPGGAQPPRPPCPRSPQAHPSSSAGTAPEVETSPAAPEPATAIQSSLLRESQASAGRTGPPQPRAASAPPTDRPLEGPLHGARRPPGTAYPGKVLVDPESGRCYFVEAPRQPRLRLLFDPESGQYVEVLLPPSPQVPPRRVYTPLALGSGLYPPAYGPLPGLSVPPSLAPAGFSGPQVPWASDAGPLDGMYYLPVSGTPSPAPPLLLCAPPNSSSSAQPGKGSLFSV

Target ORF information:

RefSeq Version	XM_005617255.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris proline-rich basic protein 1 (PROB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617255.2

ORF Insert Sequence:

ATGCTGACCG CGCTCGCCCG GCCAGCCCGG CCTGGGCTCC CGGGGCAGCC GCCTGCGGCC 
CCCGCCCGGC GTCAGGACTC CTCTGGCTCG TCAGGCTCCT ACCACACGGC TCCGGGTTCT 
CCAGGGCCCC CGGACGTTGG GCCGGACGCA GAGTGCCGGG CGCATTGGCC CGGGGTGGCC 
CCTGCGCTGG GGGCGGGCGC GCAGCCTCGC CTGTCCGTCA GCGCCCAGAA TAGCCGCCAG 
CAGCCCGGGC CCGGCTCGGG TTTCCCGCGA GGCCGGGGCT CCAGCCCGCG GCCGCCCCAG 
CCGCAGCTGC GCATGCTGCC GTCGGGGGAG ATGGAAGTCA TCTTCGGCGC AGGGGCCCCG 
TTCCGCCGCT CCGACGCGGC GGATAGCGAG GTGCAGCAAC TCACCGCCCG CGCTTTCCGC 
AGCCTCTCTC CGCCCGGGCC CGCGACTCCG GCTCCCGCGA CACCGACGCC CCAGGCCCCC 
GACGGTGGCT CCCGCTGGGC CACTTACCTA GAGGTACGGC CCCGCGGGCC GAGTCCTGGG 
ACCCCAGCGC AGTTCGAGTG TGTGGAGGTG GCGCTGGAGG AGCGTGAGGC TCCCGCCAGG 
TCCCGCACGG TGCCCAAGCG TCAGATCGAG CTGCGCCCCC GGCCCCAGAG TCCGCCGCGG 
GAGGCGGGAG CACCACCGCG CCCCCGGCTG CTCCTGCGCA CTGGCTCCCT GGACGAGTCG 
CTGGGCCGCC TGCAGGAAGC CGCTGGCCTG GTGCAGACAG CACTGGCCAG AAAACTGAGC 
CCTGCAGCCC CTGCCCCAAG CAGCGCCACC TTCGGGCCCA CGGGGCAGCC AGAGTGTGCG 
AGCCGAGAAA CGGCCCGCAG TACCCGAAAG GTCCCGGAGG AGGCCAGGTC TCGGCCACCG 
CGCGTGCACT ATGGTTCAGC ACCCACCAAG GCACCGCGGC CGTGGCCCAG CCTCCGAGAG 
CGCGCGATTC GGCGCGACAA GCCCGCGCCG GGCACCGAGC CGCTGGGTCC GGTTAGTTCC 
AGCATCTTCC TGCAGTCAGG GGAGAAGACT CGGGAGGCAC AAAGTCAGGA ACTTAAGACT 
CGGTCCCCGC GACAGACTCC GGATCGAGCC ATTCTGAGAG CACAGAGTCC GCCTTTCGAG 
TCGAGGGGCC CTCGGGAAGT TCCGAGTAAG GCTGGGAGGC CATGGAGCTC GTCCCCGCTG 
TGGCAGGCTC CAAATGGGAC CGTACGGGGT CCTCACTGCC CGTCCCCCCA GAATCCGCCC 
CCGTGGAATC GGGCTATTCG GAGGGTAAGT AGCCCGTCGT TCCCTGAAGC ATCCCGTGCA 
TGGGGAAATC GGGATCCCGC TGTCGAGGAA ACTGTCAGCA GAAGAAGCCC CTCCCCACCG 
ACTGTTTCCC CGTGGAATCA GGGTGTCGCC AGGACAAGAA GCTCATCCCC TGAAGCTCCT 
TCCCTGTGGG AGGTTCCTCA ACCGGCAGTT GGGAATACCG CAGAGGGCAG TAGGAGCGCG 
TATCCACCGG CCTTGTCCTC ATGGGAGGCT CCAGATCGTG CTAATGGGAC GTGGCATCCA 
TCTCCCCAAG ACACGTGGGG TCCCACACCA CAGGGCTCAT CCATAGTATC TACGTCTAAA 
GCTCTGAATG GGATAGGAAT GGAAGAGCTG GAGCGGCCTA GGCCGCCCAC ACCTGGGACT 
CCGGAACTCA CGGAGGCGCA AGGTCAGTCC TCGCGGGAGG TGCAGGATCT CGCCTTCCGA 
GGCAGTCAGC CGTCGCCAGA AGCGGCCGCG CCTGAGCTAA CCCTCAGTCG CCTTGTGGGC 
CCTGTGGATG CCGATGTGCC CCGGGAAGCC CTGGGCCTTG GAGAGGCCGC CTCGGGACGG 
CCTCGCGTGG CCCTCCCGCG GCCCCGCGAC GTACGCAAGA TGGTGAAGAC CACGTACGCG 
CCAAGCTTCC CCGCGGGAAC CGCAGCTTTG CGGCTGCCTG CGCCCCCTGT GGAACCCGGC 
GCGGAGGAGG GCGGCGCGTC CGGGACCGAA GCGTTGCCCG CGCTGGCGTC GCCAGCCCCA 
GCTCACTACA CTTCGGTTTA CCTCAAAGAC TTTCTTCCGG TCGTGACGCA CCCCTACGAG 
CCCCCAGAGC CGTCCCCTGA CGCGGTGCCC CGGGACGCCC CGCGGCCCAA CGGGGCCCTG 
AGGCGGAGAG CGGAGAACAG CACGGCCAAA CCCTTCGCAC GCACGGAGAT CCGCCTGCCT 
GCGGCCCTGG CCGTGGGCCG CCGGCCCGAG GGAGCGGGGG CAGTTGTGCG CGGTGCTGGC 
GGGGAGAGCC GCGAGGCTGA GGCGCGGCGC CTGGTCCCCG ACGGCGAGGG GCGGACCAGC 
CCCTTAGGCG GCGCGCGCGC GTCACCCCAG CCGCCGCCCA CGGGCCCGGG AGGGGCCCAG 
CCACCCCGAC CACCGTGCCC CCGCTCGCCT CAGGCGCACC CCAGCTCGAG CGCCGGGACA 
GCACCCGAAG TGGAGACGTC TCCTGCAGCT CCCGAGCCGG CGACTGCGAT CCAGTCGTCT 
CTCCTGCGGG AGTCCCAGGC GTCGGCCGGC AGAACCGGCC CGCCCCAACC CCGCGCTGCC 
TCAGCGCCTC CGACGGACCG GCCCCTCGAA GGCCCCCTCC ACGGGGCACG CAGGCCCCCA 
GGGACTGCGT ACCCGGGGAA GGTCCTGGTG GACCCCGAGA GCGGCCGTTG CTACTTTGTG 
GAGGCGCCGC GGCAGCCTCG GCTACGGCTA CTCTTCGACC CCGAAAGCGG GCAATACGTA 
GAGGTGCTGC TGCCGCCATC GCCCCAGGTG CCACCACGCC GCGTCTACAC CCCGCTGGCC 
CTGGGCTCTG GCCTCTACCC GCCCGCCTAT GGGCCTCTCC CGGGCCTCTC AGTGCCACCG 
TCCCTGGCCC CAGCGGGCTT CAGCGGCCCC CAGGTACCCT GGGCCTCCGA CGCGGGGCCC 
CTGGACGGGA TGTACTACCT GCCAGTGAGT GGAACCCCCA GCCCCGCTCC CCCTCTGCTC 
CTCTGTGCTC CGCCCAACAG TTCGAGTTCA GCCCAGCCCG GCAAGGGGTC CTTGTTCTCA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PROB1 ( XM_005617255.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617312.1 Canis lupus familiaris proline rich basic protein 1 (PROB1), mRNA.

Price & Availability↑

CloneID	OCe02954
Clone ID Related Accession (Same CDS sequence)	XM_005617255.2 , XM_005617255.3
Accession Version	XM_005617255.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	proline-rich basic protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617255.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACCG CGCTCGCCCG GCCAGCCCGG CCTGGGCTCC CGGGGCAGCC GCCTGCGGCC 
CCCGCCCGGC GTCAGGACTC CTCTGGCTCG TCAGGCTCCT ACCACACGGC TCCGGGTTCT 
CCAGGGCCCC CGGACGTTGG GCCGGACGCA GAGTGCCGGG CGCATTGGCC CGGGGTGGCC 
CCTGCGCTGG GGGCGGGCGC GCAGCCTCGC CTGTCCGTCA GCGCCCAGAA TAGCCGCCAG 
CAGCCCGGGC CCGGCTCGGG TTTCCCGCGA GGCCGGGGCT CCAGCCCGCG GCCGCCCCAG 
CCGCAGCTGC GCATGCTGCC GTCGGGGGAG ATGGAAGTCA TCTTCGGCGC AGGGGCCCCG 
TTCCGCCGCT CCGACGCGGC GGATAGCGAG GTGCAGCAAC TCACCGCCCG CGCTTTCCGC 
AGCCTCTCTC CGCCCGGGCC CGCGACTCCG GCTCCCGCGA CACCGACGCC CCAGGCCCCC 
GACGGTGGCT CCCGCTGGGC CACTTACCTA GAGGTACGGC CCCGCGGGCC GAGTCCTGGG 
ACCCCAGCGC AGTTCGAGTG TGTGGAGGTG GCGCTGGAGG AGCGTGAGGC TCCCGCCAGG 
TCCCGCACGG TGCCCAAGCG TCAGATCGAG CTGCGCCCCC GGCCCCAGAG TCCGCCGCGG 
GAGGCGGGAG CACCACCGCG CCCCCGGCTG CTCCTGCGCA CTGGCTCCCT GGACGAGTCG 
CTGGGCCGCC TGCAGGAAGC CGCTGGCCTG GTGCAGACAG CACTGGCCAG AAAACTGAGC 
CCTGCAGCCC CTGCCCCAAG CAGCGCCACC TTCGGGCCCA CGGGGCAGCC AGAGTGTGCG 
AGCCGAGAAA CGGCCCGCAG TACCCGAAAG GTCCCGGAGG AGGCCAGGTC TCGGCCACCG 
CGCGTGCACT ATGGTTCAGC ACCCACCAAG GCACCGCGGC CGTGGCCCAG CCTCCGAGAG 
CGCGCGATTC GGCGCGACAA GCCCGCGCCG GGCACCGAGC CGCTGGGTCC GGTTAGTTCC 
AGCATCTTCC TGCAGTCAGG GGAGAAGACT CGGGAGGCAC AAAGTCAGGA ACTTAAGACT 
CGGTCCCCGC GACAGACTCC GGATCGAGCC ATTCTGAGAG CACAGAGTCC GCCTTTCGAG 
TCGAGGGGCC CTCGGGAAGT TCCGAGTAAG GCTGGGAGGC CATGGAGCTC GTCCCCGCTG 
TGGCAGGCTC CAAATGGGAC CGTACGGGGT CCTCACTGCC CGTCCCCCCA GAATCCGCCC 
CCGTGGAATC GGGCTATTCG GAGGGTAAGT AGCCCGTCGT TCCCTGAAGC ATCCCGTGCA 
TGGGGAAATC GGGATCCCGC TGTCGAGGAA ACTGTCAGCA GAAGAAGCCC CTCCCCACCG 
ACTGTTTCCC CGTGGAATCA GGGTGTCGCC AGGACAAGAA GCTCATCCCC TGAAGCTCCT 
TCCCTGTGGG AGGTTCCTCA ACCGGCAGTT GGGAATACCG CAGAGGGCAG TAGGAGCGCG 
TATCCACCGG CCTTGTCCTC ATGGGAGGCT CCAGATCGTG CTAATGGGAC GTGGCATCCA 
TCTCCCCAAG ACACGTGGGG TCCCACACCA CAGGGCTCAT CCATAGTATC TACGTCTAAA 
GCTCTGAATG GGATAGGAAT GGAAGAGCTG GAGCGGCCTA GGCCGCCCAC ACCTGGGACT 
CCGGAACTCA CGGAGGCGCA AGGTCAGTCC TCGCGGGAGG TGCAGGATCT CGCCTTCCGA 
GGCAGTCAGC CGTCGCCAGA AGCGGCCGCG CCTGAGCTAA CCCTCAGTCG CCTTGTGGGC 
CCTGTGGATG CCGATGTGCC CCGGGAAGCC CTGGGCCTTG GAGAGGCCGC CTCGGGACGG 
CCTCGCGTGG CCCTCCCGCG GCCCCGCGAC GTACGCAAGA TGGTGAAGAC CACGTACGCG 
CCAAGCTTCC CCGCGGGAAC CGCAGCTTTG CGGCTGCCTG CGCCCCCTGT GGAACCCGGC 
GCGGAGGAGG GCGGCGCGTC CGGGACCGAA GCGTTGCCCG CGCTGGCGTC GCCAGCCCCA 
GCTCACTACA CTTCGGTTTA CCTCAAAGAC TTTCTTCCGG TCGTGACGCA CCCCTACGAG 
CCCCCAGAGC CGTCCCCTGA CGCGGTGCCC CGGGACGCCC CGCGGCCCAA CGGGGCCCTG 
AGGCGGAGAG CGGAGAACAG CACGGCCAAA CCCTTCGCAC GCACGGAGAT CCGCCTGCCT 
GCGGCCCTGG CCGTGGGCCG CCGGCCCGAG GGAGCGGGGG CAGTTGTGCG CGGTGCTGGC 
GGGGAGAGCC GCGAGGCTGA GGCGCGGCGC CTGGTCCCCG ACGGCGAGGG GCGGACCAGC 
CCCTTAGGCG GCGCGCGCGC GTCACCCCAG CCGCCGCCCA CGGGCCCGGG AGGGGCCCAG 
CCACCCCGAC CACCGTGCCC CCGCTCGCCT CAGGCGCACC CCAGCTCGAG CGCCGGGACA 
GCACCCGAAG TGGAGACGTC TCCTGCAGCT CCCGAGCCGG CGACTGCGAT CCAGTCGTCT 
CTCCTGCGGG AGTCCCAGGC GTCGGCCGGC AGAACCGGCC CGCCCCAACC CCGCGCTGCC 
TCAGCGCCTC CGACGGACCG GCCCCTCGAA GGCCCCCTCC ACGGGGCACG CAGGCCCCCA 
GGGACTGCGT ACCCGGGGAA GGTCCTGGTG GACCCCGAGA GCGGCCGTTG CTACTTTGTG 
GAGGCGCCGC GGCAGCCTCG GCTACGGCTA CTCTTCGACC CCGAAAGCGG GCAATACGTA 
GAGGTGCTGC TGCCGCCATC GCCCCAGGTG CCACCACGCC GCGTCTACAC CCCGCTGGCC 
CTGGGCTCTG GCCTCTACCC GCCCGCCTAT GGGCCTCTCC CGGGCCTCTC AGTGCCACCG 
TCCCTGGCCC CAGCGGGCTT CAGCGGCCCC CAGGTACCCT GGGCCTCCGA CGCGGGGCCC 
CTGGACGGGA TGTACTACCT GCCAGTGAGT GGAACCCCCA GCCCCGCTCC CCCTCTGCTC 
CTCTGTGCTC CGCCCAACAG TTCGAGTTCA GCCCAGCCCG GCAAGGGGTC CTTGTTCTCA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617312.1
CDS	1783..4848
Translation	MLTALARPARPGLPGQPPAAPARRQDSSGSSGSYHTAPGSPGPPDVGPDAECRAHWPGVAPALGAGAQPRLSVSAQNSRQQPGPGSGFPRGRGSSPRPPQPQLRMLPSGEMEVIFGAGAPFRRSDAADSEVQQLTARAFRSLSPPGPATPAPATPTPQAPDGGSRWATYLEVRPRGPSPGTPAQFECVEVALEEREAPARSRTVPKRQIELRPRPQSPPREAGAPPRPRLLLRTGSLDESLGRLQEAAGLVQTALARKLSPAAPAPSSATFGPTGQPECASRETARSTRKVPEEARSRPPRVHYGSAPTKAPRPWPSLRERAIRRDKPAPGTEPLGPVSSSIFLQSGEKTREAQSQELKTRSPRQTPDRAILRAQSPPFESRGPREVPSKAGRPWSSSPLWQAPNGTVRGPHCPSPQNPPPWNRAIRRVSSPSFPEASRAWGNRDPAVEETVSRRSPSPPTVSPWNQGVARTRSSSPEAPSLWEVPQPAVGNTAEGSRSAYPPALSSWEAPDRANGTWHPSPQDTWGPTPQGSSIVSTSKALNGIGMEELERPRPPTPGTPELTEAQGQSSREVQDLAFRGSQPSPEAAAPELTLSRLVGPVDADVPREALGLGEAASGRPRVALPRPRDVRKMVKTTYAPSFPAGTAALRLPAPPVEPGAEEGGASGTEALPALASPAPAHYTSVYLKDFLPVVTHPYEPPEPSPDAVPRDAPRPNGALRRRAENSTAKPFARTEIRLPAALAVGRRPEGAGAVVRGAGGESREAEARRLVPDGEGRTSPLGGARASPQPPPTGPGGAQPPRPPCPRSPQAHPSSSAGTAPEVETSPAAPEPATAIQSSLLRESQASAGRTGPPQPRAASAPPTDRPLEGPLHGARRPPGTAYPGKVLVDPESGRCYFVEAPRQPRLRLLFDPESGQYVEVLLPPSPQVPPRRVYTPLALGSGLYPPAYGPLPGLSVPPSLAPAGFSGPQVPWASDAGPLDGMYYLPVSGTPSPAPPLLLCAPPNSSSSAQPGKGSLFSV

Target ORF information:

RefSeq Version	XM_005617255.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris proline rich basic protein 1 (PROB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617255.3

ORF Insert Sequence:

ATGCTGACCG CGCTCGCCCG GCCAGCCCGG CCTGGGCTCC CGGGGCAGCC GCCTGCGGCC 
CCCGCCCGGC GTCAGGACTC CTCTGGCTCG TCAGGCTCCT ACCACACGGC TCCGGGTTCT 
CCAGGGCCCC CGGACGTTGG GCCGGACGCA GAGTGCCGGG CGCATTGGCC CGGGGTGGCC 
CCTGCGCTGG GGGCGGGCGC GCAGCCTCGC CTGTCCGTCA GCGCCCAGAA TAGCCGCCAG 
CAGCCCGGGC CCGGCTCGGG TTTCCCGCGA GGCCGGGGCT CCAGCCCGCG GCCGCCCCAG 
CCGCAGCTGC GCATGCTGCC GTCGGGGGAG ATGGAAGTCA TCTTCGGCGC AGGGGCCCCG 
TTCCGCCGCT CCGACGCGGC GGATAGCGAG GTGCAGCAAC TCACCGCCCG CGCTTTCCGC 
AGCCTCTCTC CGCCCGGGCC CGCGACTCCG GCTCCCGCGA CACCGACGCC CCAGGCCCCC 
GACGGTGGCT CCCGCTGGGC CACTTACCTA GAGGTACGGC CCCGCGGGCC GAGTCCTGGG 
ACCCCAGCGC AGTTCGAGTG TGTGGAGGTG GCGCTGGAGG AGCGTGAGGC TCCCGCCAGG 
TCCCGCACGG TGCCCAAGCG TCAGATCGAG CTGCGCCCCC GGCCCCAGAG TCCGCCGCGG 
GAGGCGGGAG CACCACCGCG CCCCCGGCTG CTCCTGCGCA CTGGCTCCCT GGACGAGTCG 
CTGGGCCGCC TGCAGGAAGC CGCTGGCCTG GTGCAGACAG CACTGGCCAG AAAACTGAGC 
CCTGCAGCCC CTGCCCCAAG CAGCGCCACC TTCGGGCCCA CGGGGCAGCC AGAGTGTGCG 
AGCCGAGAAA CGGCCCGCAG TACCCGAAAG GTCCCGGAGG AGGCCAGGTC TCGGCCACCG 
CGCGTGCACT ATGGTTCAGC ACCCACCAAG GCACCGCGGC CGTGGCCCAG CCTCCGAGAG 
CGCGCGATTC GGCGCGACAA GCCCGCGCCG GGCACCGAGC CGCTGGGTCC GGTTAGTTCC 
AGCATCTTCC TGCAGTCAGG GGAGAAGACT CGGGAGGCAC AAAGTCAGGA ACTTAAGACT 
CGGTCCCCGC GACAGACTCC GGATCGAGCC ATTCTGAGAG CACAGAGTCC GCCTTTCGAG 
TCGAGGGGCC CTCGGGAAGT TCCGAGTAAG GCTGGGAGGC CATGGAGCTC GTCCCCGCTG 
TGGCAGGCTC CAAATGGGAC CGTACGGGGT CCTCACTGCC CGTCCCCCCA GAATCCGCCC 
CCGTGGAATC GGGCTATTCG GAGGGTAAGT AGCCCGTCGT TCCCTGAAGC ATCCCGTGCA 
TGGGGAAATC GGGATCCCGC TGTCGAGGAA ACTGTCAGCA GAAGAAGCCC CTCCCCACCG 
ACTGTTTCCC CGTGGAATCA GGGTGTCGCC AGGACAAGAA GCTCATCCCC TGAAGCTCCT 
TCCCTGTGGG AGGTTCCTCA ACCGGCAGTT GGGAATACCG CAGAGGGCAG TAGGAGCGCG 
TATCCACCGG CCTTGTCCTC ATGGGAGGCT CCAGATCGTG CTAATGGGAC GTGGCATCCA 
TCTCCCCAAG ACACGTGGGG TCCCACACCA CAGGGCTCAT CCATAGTATC TACGTCTAAA 
GCTCTGAATG GGATAGGAAT GGAAGAGCTG GAGCGGCCTA GGCCGCCCAC ACCTGGGACT 
CCGGAACTCA CGGAGGCGCA AGGTCAGTCC TCGCGGGAGG TGCAGGATCT CGCCTTCCGA 
GGCAGTCAGC CGTCGCCAGA AGCGGCCGCG CCTGAGCTAA CCCTCAGTCG CCTTGTGGGC 
CCTGTGGATG CCGATGTGCC CCGGGAAGCC CTGGGCCTTG GAGAGGCCGC CTCGGGACGG 
CCTCGCGTGG CCCTCCCGCG GCCCCGCGAC GTACGCAAGA TGGTGAAGAC CACGTACGCG 
CCAAGCTTCC CCGCGGGAAC CGCAGCTTTG CGGCTGCCTG CGCCCCCTGT GGAACCCGGC 
GCGGAGGAGG GCGGCGCGTC CGGGACCGAA GCGTTGCCCG CGCTGGCGTC GCCAGCCCCA 
GCTCACTACA CTTCGGTTTA CCTCAAAGAC TTTCTTCCGG TCGTGACGCA CCCCTACGAG 
CCCCCAGAGC CGTCCCCTGA CGCGGTGCCC CGGGACGCCC CGCGGCCCAA CGGGGCCCTG 
AGGCGGAGAG CGGAGAACAG CACGGCCAAA CCCTTCGCAC GCACGGAGAT CCGCCTGCCT 
GCGGCCCTGG CCGTGGGCCG CCGGCCCGAG GGAGCGGGGG CAGTTGTGCG CGGTGCTGGC 
GGGGAGAGCC GCGAGGCTGA GGCGCGGCGC CTGGTCCCCG ACGGCGAGGG GCGGACCAGC 
CCCTTAGGCG GCGCGCGCGC GTCACCCCAG CCGCCGCCCA CGGGCCCGGG AGGGGCCCAG 
CCACCCCGAC CACCGTGCCC CCGCTCGCCT CAGGCGCACC CCAGCTCGAG CGCCGGGACA 
GCACCCGAAG TGGAGACGTC TCCTGCAGCT CCCGAGCCGG CGACTGCGAT CCAGTCGTCT 
CTCCTGCGGG AGTCCCAGGC GTCGGCCGGC AGAACCGGCC CGCCCCAACC CCGCGCTGCC 
TCAGCGCCTC CGACGGACCG GCCCCTCGAA GGCCCCCTCC ACGGGGCACG CAGGCCCCCA 
GGGACTGCGT ACCCGGGGAA GGTCCTGGTG GACCCCGAGA GCGGCCGTTG CTACTTTGTG 
GAGGCGCCGC GGCAGCCTCG GCTACGGCTA CTCTTCGACC CCGAAAGCGG GCAATACGTA 
GAGGTGCTGC TGCCGCCATC GCCCCAGGTG CCACCACGCC GCGTCTACAC CCCGCTGGCC 
CTGGGCTCTG GCCTCTACCC GCCCGCCTAT GGGCCTCTCC CGGGCCTCTC AGTGCCACCG 
TCCCTGGCCC CAGCGGGCTT CAGCGGCCCC CAGGTACCCT GGGCCTCCGA CGCGGGGCCC 
CTGGACGGGA TGTACTACCT GCCAGTGAGT GGAACCCCCA GCCCCGCTCC CCCTCTGCTC 
CTCTGTGCTC CGCCCAACAG TTCGAGTTCA GCCCAGCCCG GCAAGGGGTC CTTGTTCTCA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PROB1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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