FREM3 cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset)

Gene
Clones
gRNAs

Summary

Gene Symbol	FREM3
Entrez Gene ID	100405327
Full Name	FRAS1 related extracellular matrix 3
Gene Type	protein-coding
Organism	Callithrix jacchus(white-tufted-ear marmoset)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002745319.3	XP_002745365.1	FRAS1-related extracellular matrix protein 3

Pathways from BioSystems
Gene Ontology

The following FREM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCb02810	XM_002745319.3 Latest version!	Callithrix jacchus FRAS1 related extracellular matrix 3 (FREM3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FREM3 ( XM_002745319.3 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_002745365.1 Callithrix jacchus FRAS1 related extracellular matrix 3 (FREM3), mRNA.

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CloneID	OCb02810
Clone ID Related Accession (Same CDS sequence)	XM_002745319.3
Accession Version	XM_002745319.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1472486400000
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	FRAS1-related extracellular matrix protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013898.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 31, 2016 this sequence version replaced XM_002745319.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CTTCTCGACA CCCAATTGGG ACGCCCCGGC AGCTTGTTGT GGCGCTCGCC 
TGCCTACTGC TGAGTCGACC CGCGCTGCAG GGACCGCCAT CCTCGCTTGG GACCGAGCCC 
CACTCGGCGC TTTACCTGCC TGTCAGGGGT GAGCAGAACG GCAGTAGCCC CGACGCCCCC 
AGCGTGCTGA TTGCCAACCC GGGGCTCCGG GTGCCCCTGG GCCGCTCCCT TTGGCTCGAC 
CCTCTCCGGG ACCTGGTGAT TGGAGTGCAG CCGGGGGACC GGTGCGAAGT CACGGTCCTG 
GACTCCCTGC CGCCTTTCAA GGGCGCTCTC TCCCCGCACC GCTTCCCCTG CGCCTTCGGG 
CCCCGCCAGG TCCAGTACAC TCACTTCGGC TCCCACAGCC CTGGACGCGC CCGGGTGCTG 
CTGCAGCTGC GCTACGACGC CCCAACTCAC ACGCTGGTGC TGCCCTTCAC ACTGGCGGTG 
GACGTGGTCT TTTCCCAGCT GGAGCTGGTG ACGCGCAACA GGCCTTTGGT GGTGGAGAAG 
CTGCGGGGCT GCAGCCGCGC CATCGACAAG AGAGTGCTGG ACTTCGCTTC CATGAAGTCA 
GGAGCCAAGG CCACCGGCAG GTGCCAGCTC ACCCCGCTTC CTCACGAGGG CGGCCCCATG 
CCCAAGTACG GGCGCTTAGT GGACGCACTG GGGGCTCCAC TCCTCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTCGTCCA GGCAGGGGTG CTCTATCAGC ACACCGCCGC CTCCTCGCCC 
AACCGGGACT ACGTGCCCAT GATGGTGGAG CTGCTGGGGC CGGAGGGCCA AGACGCTGGG 
TCCCTGGGAG TGCTGGCCCG GGAGCACTTC CAGCTGCTGG TGAGGATCCG CGGGGGAGCC 
AAGAACACAG CGCCCAGGCC CAGCTTCGTG GCCACGATGA TGATGGAGGT GAATCCATTG 
GTGCTGACAG CCCTGACGCC TGACGCACTG GCCGCGGAGG ACGTTGAGTC AGACCCCGGC 
GACCTGGTGT TCAACATTCT CAACGCCTCC ACTCCCCCAC CACCAGGGCA CCCTGGGCAG 
CAGGGCTACA TGGTCAGCAC CGATGACCCT CTAGGCCTTC CAGTCTCCTT CTTCACCCAG 
CAGGAGCTGC GGGAGCTGAA GATTGCCTAT CAGCCCCCTA CAGAGAACTC CCATGGGGAG 
CGCCTCTTTC AGCTGGAGCT GGAGGTGGTG GACGGAGATG GCTCCACCTC AGACCCCTTT 
GCCTTCATGG TGATAGTGAA ATCCATGAAC ACTCTGGTCC CAGTGGCTAG CCATACCAGG 
GGACTTGTGC TTTTTGAAGG TCAGTCAAGG CCCTTGTCCA GCGTTCCTAT CAGTGATAAA 
GATAACGTGG AAGAAATGAA AATGGCTGCA GTCAGAGGCT TGAGACATGG GCAGCTGGTG 
GTGTTTGGGG CACCTGCTGG GTGCAAGTAT TTCACACCAG CAGACCTGGC AGCAGGACGA 
GTGGTGTATC AGCACGACGG CAGCAACACC TACAGTGACA ACATCATCTT CCAGATGGAG 
GATGGGTACC ACCAAGTGGA ATTCCTCTTT CCCCTCACCA TCCTACCTGT GGATGATGAA 
CCACCAATGG TCAGTACTAA CACAGGACTC TCAGTGACTG AAGGGCAGGT GGTCCAGATC 
TCTCCCTTTG TACTGTGTGC TACTGATATT GACTCTGAGG ACTCAACCAT TCGCTTTGTG 
CTGGAGAACC AGCCCCTGGA AGGAAATGAG GAAGAGCCAG AGTGGGAACT GGCCCCTGGT 
TCCTCTCACT CAGGCCACTA CCTGGGGGAC ATGTTGCTGC GGCAGGCTGA ACTACCTCTC 
TCCACTGAAG ATGAAGAATG GCACTACAAG GAAAAGGAAG GGCTTTATGA GAAAGTGGTG 
ACTCAGTGGC TACAGAGAGA CATAACAGAA GGGAGACTCT TCTACCGTCA TCTTGGACCA 
CACAGCCCTC AATCTGTAAT GGTCCAGTTG GCTTTCCATG TGCAGGATGA CCATGACCCA 
CCCAATCTAT CCAACCAGCA CATTTTCACC ATCAAGGTCC AGCCAGTGGA TATACTGAGT 
CCACAGCTGT ATCCAGGAAC TACACTAGAA ATGACTGTAC AAGAATACCA ACTCACTCAC 
TTCCAGAAGA ATTTCCTCCG ATACATTGAC CAGGATTCTG ATGACCCGAA CCTATGGTAC 
ACCCTACTGA CACTCCCAAC TGACACAGAT GATAACCACC AAGTCCGGGC TGGAGAAATA 
GTGCTCACTG ATTCACCAGA CACACTCATT ATGCATTTCA CCCAAGCCCA GGTAAATCAG 
CATAAAGTTG CCTACAAGCC TCCACAGAAG TTGGGTATTG CACCACGGGT TGTGCAGTTT 
ACCTACCAGG TGGAAGATGC TGCAGGCAAT AGTGTGCCAG GCACATTCAC ATTATTCCTG 
CAACCTGTGG ACAACCAGCC CCCAGAAGTC AACAACAGCG GCTTTACTGT CTTAGAGGGA 
GGCAGCTTTA ACCTCAGCAG TAATGAACTG CATGTTACAG ACCCAGACAC AGACACTGAC 
CAAATTGTCT TCACATTAGT CCAGGGTCCC CAGCATGGAC ACTTACAGTA CTTTAAAAAA 
TGTATGGTCC CAGGGGAATC TTTCATGCAA GCTGATATTA TTAATGGGAG TGTCTCTTAC 
CATCATGACA GAGACCAGAC TACTACTAGG GACACTTTCC ATCTGGAGGT AAGTGATGGG 
GTGCACCTTA TACCCATCAC TGTCCCAATT TCCGTGCACC CCGATGTGGC TAACAAAAGT 
CCTAGGATCT CTATTACAAG TAGTTTCTTA TTGGATGTTT CCATAGATGT ACTAGAGAAT 
AAAGCCACTG AGATTACCAT GGGTGTCATC CATGGAAAAA GGAAGGATGT CCATGACTTG 
ATGCTGTCTT TCATTGTAAA GGACAGCCCC AAATTGGGCA CTATTCTGGT AAATGGTTTG 
TCCAGAGAAC GATTCACCCA AGAAGACCTC ATCAGTGGGA GAGTAGCCTA TGTCCACACT 
GGAGGTGAAG TTGGTTTTCA AAAGCAGCAT GATGCCTTCA GCCTCATCCT CTCCAAGGAT 
TCTTACCAAT GGGTAGTGGG AAATAGCATA GTAGAGAGAA TGCAGGTACA AGTAACTGTG 
CTGCCTGTGG ACAATGTGGG ACCCAAGGTC TTGGTGGGGG AGTCCTTCAT TGTCTATGAA 
GGTGAGAAGA ATCCCTTGAC CTTACAACAT CTCCATATTG AAGATGTGGA CACCCCCCAA 
GATGAACTCC TATGCACAGT GACTAGTCAG CCAGCCTCTG GCTACCTGGA AAAGATTGCA 
GCAACTACTG GTTCAAAAAT GTCCCAGTCT GGTAGCCCCA TCAGTGCTTT CTCCCTCAGA 
GATGTTCAAG TGAGGAATAT CAATTATGTA CAGAGTATTC ACAAGGGAGT AGAGCCACAA 
GAGGATCAAT TCACCTTTTA TTGCTCTGAC GGCATCAACT CCTCCCCAAA TGTCTTCTTC 
CCTATAATCA TCCTCCCTAC CAATGATGAG CAGCCTGAAC TTTCTGTCCA TGAGTTTGTG 
GTACTAGAGG GGATGAGCCT GGTCATAGAC ACCCAGCTGC TCAGTGGAGC AGATGCTGAC 
CTGCCACGGA ATGAGCTGCA CTTTCAACTC ACAGCCCTCC CTCGGCATGG ACGAATCATA 
CAGCAGCTGG ATACAGGTAG CCGGCCCATC CACAGCTTCA CCCTCCAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAT TCAGAGACCA CAGAGGACAG TTTTGAAGTC 
TGGCTGAGTG ACGGCAAGCA CACCACCCAC AGGAAGGTAC CCATTGTAGT GACCCTAGTG 
GATGATGAAA CCCCTCAACT GACTGTCAAC AATGGGCTGA AAGTAGAGAA AGGACAATCT 
GAGATCATCA CAAATCAGAT CCTCAAGGCC ACAGATCTTG ACTCAAATGA TAAGAGCCTC 
AGTTTTGTCC TCCATTCTGG GCCTCAACAA GGTCTTCTAC AGAGACTGAG AGAACCCAGA 
GGAGAAGTGA GAAACAATCT CACTCTGGGA ATGAACTTTA CCCAGGATGA GATTGACAGA 
GGCCTCATCT GCTATACCCA CACAGGCCAA GAAGGGATTG TTGACTTTAT CAAGTTTGAT 
GTGACTGATG GGGCTAACAC TTTGACAGAC CACTGCTTCT ATGTCACCAT TGGCAACTTA 
GACAAAGTCT TCCCTGAGGT AATCAGCAAA AGGGTCACCT TGACAGAAGG TGCCAGAGTG 
ACCCTTACCG ACAGTCTGCT TAGCAACAGT GACGTCAACA GCTCTGATGA ACATCACTTT 
AGCATCACAC GGGCTCCAAG CCTGGGTCAC CTGGAAAGTT CTGACCATGC TGGGGAACCC 
ATTGCCTCTT TCACTCAGCT TCAGTTGACT AGCAACAAAA TATCCTATGT CCATACTTCA 
AATGATGAGA AAAAGATGGA CAGCTTTGAG TTTCAGGTGA TCAGTAAACA CTACCCTGTG 
TTCAGAACCT TCAGGGTCTT CATCACTGAT GTGGATAATA AGAAACCTAT CTTGATCATC 
CATAGACTAA CACTGCAGGA AGAGGATAGC CAACTGATCA CTCCCCTTGA GCTGACAGTG 
GAAGATGAGG ATACTCCAGA TGATCTTATT CTCTTTACCA TCACCCAGGT CCCCAGGCAT 
GGCAAGATTT TGTACAATGG TAACCATCCT GTGACCACTT TCACCAAGCA AGACCTGAAC 
AAGAACCTGA TTAGCTACAA GCATGATGGT AGTGAGACTG CTGAAGACAG TTTCTCCTTG 
ACTGTGACAG ATGGCACTCA CACTGGTTTC TATGTCCTCC CAGACACTGC CCTGGAGACA 
CACAAACCTC AAGTAATGAG GGTCCAGATA AGATCATTAG ATAATAGGCT TCCCCTGATT 
ACTATTAACA GGGGTGCCCC AACCTTGAAA CGCCTTCACA CTGGACACAT GGGCTTCCTG 
ATTACCAGCA AGTCTCTGAA GGCAGAAGAT CAGGACAGTC CCCACAGGCT TCTGAAGTAT 
AAAGTGACCA GAGGACCACA GCATGGCTTC ATCACCAAAA CTGGCCTTGG AAACCAGATC 
ACTCGAGTGT TTACACAAGC TGACATTGAT GAGATGAAGG TCTACTATGT CTTGAATGAG 
GGCAGCAGCA CATCAAAGGA CATCTTCTAT TTCTCTGTTG AAGACAGTGG AGGGAATAAA 
CTAACAAATC AGCCTTTCCG TCTAAACTGG GCTTGGATTT GTTTGGAGAA AGAGTACTAC 
ATCGTGGATG AAGACTCCAC ATTCTTAGAA GTGACCCTTA TACGCAGAGG ACACCTTGGA 
GAAACATCAT TTATCAGCAT TGGTACCAAA GATGAAACTG CAAAAAAAGA TAAAGATTTT 
AAATGGAAGA GCAATAAACA GGTCCAATTC AATCCTGGAC AGACTACAGC CACATTAAGG 
GTGAGAATTA TACCTGACAA TGAATATGAG ACTTCAGAGA CCTTCCAGAT CATTCTGTCA 
GAACCTCTCA TGGCTGCACT GGAGTTTCCA GAAATGGCAA CAGTTGAAAT TGTAGACCCT 
GGAGATGAAT CAACAGTTTA TATTCCAGAG GCGGAATACA AAGTAGAAGA GGATGTTGGG 
GAACTTTTGA TTCCAGTAAG ACGATCTGGA GATGCAAGCC AGGAACTAAT GGTCATTTGC 
TCCACATGTC AAGGTTCTGC CACAGGCACA GTTTCTTCCA CAGTATTATC ATTCTCTGAT 
TACATCTCAC GTCCAGAAGA TCACACCAGC ATCCTCCACT TTGGCAAGAA CGAGACACAG 
AAGACCTGCA GGGTCCTGAT CATCAACGAC TCCCTTTATG AAGAGGAGGA ATTCTTCAGT 
GTTTCACTGA GGCTGCCAGT GGGAGGACAG CTGGGAGCCA GATTCCCCAC AACTAAGGTG 
ACTATCATGG CTGATCGTTA TGATGAACCT GCCCTGCACT TTGGGGATGC TGAATATCAT 
GTCAATGAAA GTGCTGGCTA TGTGGAGATT TGTGTTTGGA GAAGAGGCAC TGATCTTTCC 
CAACCATCAT CCATCACCGT GTTCTCCAGA AAGTCAGAGA AGGAGTCAGC AGAAGCTGGA 
ACAGATTATG TTGGCATCAA CCGAAATCTG GACTTTGCTC CAGGAGTGCG CATGCAGACA 
TTCCGAGTGA CTATCCTTGA TGACATGGGA CAGCCCACCT TGGAAGGCCC AGAGAAGTTT 
GAATTACACC TTCAGATGCC GGTGGGTGCA GTGCTTGGAG AACCAAATAA AACTACCGTT 
TTCATCAAGG ATACCATCAC TGACTGTAAG CTGAGTGCTT GTAGTTCCTT TGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002745365.1
CDS	98..6514
Translation	MAGASRHPIGTPRQLVVALACLLLSRPALQGPPSSLGTEPHSALYLPVRGEQNGSSPDAPSVLIANPGLRVPLGRSLWLDPLRDLVIGVQPGDRCEVTVLDSLPPFKGALSPHRFPCAFGPRQVQYTHFGSHSPGRARVLLQLRYDAPTHTLVLPFTLAVDVVFSQLELVTRNRPLVVEKLRGCSRAIDKRVLDFASMKSGAKATGRCQLTPLPHEGGPMPKYGRLVDALGAPLLRGKGVDCEAFVQAGVLYQHTAASSPNRDYVPMMVELLGPEGQDAGSLGVLAREHFQLLVRIRGGAKNTAPRPSFVATMMMEVNPLVLTALTPDALAAEDVESDPGDLVFNILNASTPPPPGHPGQQGYMVSTDDPLGLPVSFFTQQELRELKIAYQPPTENSHGERLFQLELEVVDGDGSTSDPFAFMVIVKSMNTLVPVASHTRGLVLFEGQSRPLSSVPISDKDNVEEMKMAAVRGLRHGQLVVFGAPAGCKYFTPADLAAGRVVYQHDGSNTYSDNIIFQMEDGYHQVEFLFPLTILPVDDEPPMVSTNTGLSVTEGQVVQISPFVLCATDIDSEDSTIRFVLENQPLEGNEEEPEWELAPGSSHSGHYLGDMLLRQAELPLSTEDEEWHYKEKEGLYEKVVTQWLQRDITEGRLFYRHLGPHSPQSVMVQLAFHVQDDHDPPNLSNQHIFTIKVQPVDILSPQLYPGTTLEMTVQEYQLTHFQKNFLRYIDQDSDDPNLWYTLLTLPTDTDDNHQVRAGEIVLTDSPDTLIMHFTQAQVNQHKVAYKPPQKLGIAPRVVQFTYQVEDAAGNSVPGTFTLFLQPVDNQPPEVNNSGFTVLEGGSFNLSSNELHVTDPDTDTDQIVFTLVQGPQHGHLQYFKKCMVPGESFMQADIINGSVSYHHDRDQTTTRDTFHLEVSDGVHLIPITVPISVHPDVANKSPRISITSSFLLDVSIDVLENKATEITMGVIHGKRKDVHDLMLSFIVKDSPKLGTILVNGLSRERFTQEDLISGRVAYVHTGGEVGFQKQHDAFSLILSKDSYQWVVGNSIVERMQVQVTVLPVDNVGPKVLVGESFIVYEGEKNPLTLQHLHIEDVDTPQDELLCTVTSQPASGYLEKIAATTGSKMSQSGSPISAFSLRDVQVRNINYVQSIHKGVEPQEDQFTFYCSDGINSSPNVFFPIIILPTNDEQPELSVHEFVVLEGMSLVIDTQLLSGADADLPRNELHFQLTALPRHGRIIQQLDTGSRPIHSFTLQEIQEASTIVYEHDDSETTEDSFEVWLSDGKHTTHRKVPIVVTLVDDETPQLTVNNGLKVEKGQSEIITNQILKATDLDSNDKSLSFVLHSGPQQGLLQRLREPRGEVRNNLTLGMNFTQDEIDRGLICYTHTGQEGIVDFIKFDVTDGANTLTDHCFYVTIGNLDKVFPEVISKRVTLTEGARVTLTDSLLSNSDVNSSDEHHFSITRAPSLGHLESSDHAGEPIASFTQLQLTSNKISYVHTSNDEKKMDSFEFQVISKHYPVFRTFRVFITDVDNKKPILIIHRLTLQEEDSQLITPLELTVEDEDTPDDLILFTITQVPRHGKILYNGNHPVTTFTKQDLNKNLISYKHDGSETAEDSFSLTVTDGTHTGFYVLPDTALETHKPQVMRVQIRSLDNRLPLITINRGAPTLKRLHTGHMGFLITSKSLKAEDQDSPHRLLKYKVTRGPQHGFITKTGLGNQITRVFTQADIDEMKVYYVLNEGSSTSKDIFYFSVEDSGGNKLTNQPFRLNWAWICLEKEYYIVDEDSTFLEVTLIRRGHLGETSFISIGTKDETAKKDKDFKWKSNKQVQFNPGQTTATLRVRIIPDNEYETSETFQIILSEPLMAALEFPEMATVEIVDPGDESTVYIPEAEYKVEEDVGELLIPVRRSGDASQELMVICSTCQGSATGTVSSTVLSFSDYISRPEDHTSILHFGKNETQKTCRVLIINDSLYEEEEFFSVSLRLPVGGQLGARFPTTKVTIMADRYDEPALHFGDAEYHVNESAGYVEICVWRRGTDLSQPSSITVFSRKSEKESAEAGTDYVGINRNLDFAPGVRMQTFRVTILDDMGQPTLEGPEKFELHLQMPVGAVLGEPNKTTVFIKDTITDCKLSACSSFD

Target ORF information:

RefSeq Version	XM_002745319.3
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus FRAS1 related extracellular matrix 3 (FREM3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002745319.3

ORF Insert Sequence:

ATGGCGGGGG CTTCTCGACA CCCAATTGGG ACGCCCCGGC AGCTTGTTGT GGCGCTCGCC 
TGCCTACTGC TGAGTCGACC CGCGCTGCAG GGACCGCCAT CCTCGCTTGG GACCGAGCCC 
CACTCGGCGC TTTACCTGCC TGTCAGGGGT GAGCAGAACG GCAGTAGCCC CGACGCCCCC 
AGCGTGCTGA TTGCCAACCC GGGGCTCCGG GTGCCCCTGG GCCGCTCCCT TTGGCTCGAC 
CCTCTCCGGG ACCTGGTGAT TGGAGTGCAG CCGGGGGACC GGTGCGAAGT CACGGTCCTG 
GACTCCCTGC CGCCTTTCAA GGGCGCTCTC TCCCCGCACC GCTTCCCCTG CGCCTTCGGG 
CCCCGCCAGG TCCAGTACAC TCACTTCGGC TCCCACAGCC CTGGACGCGC CCGGGTGCTG 
CTGCAGCTGC GCTACGACGC CCCAACTCAC ACGCTGGTGC TGCCCTTCAC ACTGGCGGTG 
GACGTGGTCT TTTCCCAGCT GGAGCTGGTG ACGCGCAACA GGCCTTTGGT GGTGGAGAAG 
CTGCGGGGCT GCAGCCGCGC CATCGACAAG AGAGTGCTGG ACTTCGCTTC CATGAAGTCA 
GGAGCCAAGG CCACCGGCAG GTGCCAGCTC ACCCCGCTTC CTCACGAGGG CGGCCCCATG 
CCCAAGTACG GGCGCTTAGT GGACGCACTG GGGGCTCCAC TCCTCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTCGTCCA GGCAGGGGTG CTCTATCAGC ACACCGCCGC CTCCTCGCCC 
AACCGGGACT ACGTGCCCAT GATGGTGGAG CTGCTGGGGC CGGAGGGCCA AGACGCTGGG 
TCCCTGGGAG TGCTGGCCCG GGAGCACTTC CAGCTGCTGG TGAGGATCCG CGGGGGAGCC 
AAGAACACAG CGCCCAGGCC CAGCTTCGTG GCCACGATGA TGATGGAGGT GAATCCATTG 
GTGCTGACAG CCCTGACGCC TGACGCACTG GCCGCGGAGG ACGTTGAGTC AGACCCCGGC 
GACCTGGTGT TCAACATTCT CAACGCCTCC ACTCCCCCAC CACCAGGGCA CCCTGGGCAG 
CAGGGCTACA TGGTCAGCAC CGATGACCCT CTAGGCCTTC CAGTCTCCTT CTTCACCCAG 
CAGGAGCTGC GGGAGCTGAA GATTGCCTAT CAGCCCCCTA CAGAGAACTC CCATGGGGAG 
CGCCTCTTTC AGCTGGAGCT GGAGGTGGTG GACGGAGATG GCTCCACCTC AGACCCCTTT 
GCCTTCATGG TGATAGTGAA ATCCATGAAC ACTCTGGTCC CAGTGGCTAG CCATACCAGG 
GGACTTGTGC TTTTTGAAGG TCAGTCAAGG CCCTTGTCCA GCGTTCCTAT CAGTGATAAA 
GATAACGTGG AAGAAATGAA AATGGCTGCA GTCAGAGGCT TGAGACATGG GCAGCTGGTG 
GTGTTTGGGG CACCTGCTGG GTGCAAGTAT TTCACACCAG CAGACCTGGC AGCAGGACGA 
GTGGTGTATC AGCACGACGG CAGCAACACC TACAGTGACA ACATCATCTT CCAGATGGAG 
GATGGGTACC ACCAAGTGGA ATTCCTCTTT CCCCTCACCA TCCTACCTGT GGATGATGAA 
CCACCAATGG TCAGTACTAA CACAGGACTC TCAGTGACTG AAGGGCAGGT GGTCCAGATC 
TCTCCCTTTG TACTGTGTGC TACTGATATT GACTCTGAGG ACTCAACCAT TCGCTTTGTG 
CTGGAGAACC AGCCCCTGGA AGGAAATGAG GAAGAGCCAG AGTGGGAACT GGCCCCTGGT 
TCCTCTCACT CAGGCCACTA CCTGGGGGAC ATGTTGCTGC GGCAGGCTGA ACTACCTCTC 
TCCACTGAAG ATGAAGAATG GCACTACAAG GAAAAGGAAG GGCTTTATGA GAAAGTGGTG 
ACTCAGTGGC TACAGAGAGA CATAACAGAA GGGAGACTCT TCTACCGTCA TCTTGGACCA 
CACAGCCCTC AATCTGTAAT GGTCCAGTTG GCTTTCCATG TGCAGGATGA CCATGACCCA 
CCCAATCTAT CCAACCAGCA CATTTTCACC ATCAAGGTCC AGCCAGTGGA TATACTGAGT 
CCACAGCTGT ATCCAGGAAC TACACTAGAA ATGACTGTAC AAGAATACCA ACTCACTCAC 
TTCCAGAAGA ATTTCCTCCG ATACATTGAC CAGGATTCTG ATGACCCGAA CCTATGGTAC 
ACCCTACTGA CACTCCCAAC TGACACAGAT GATAACCACC AAGTCCGGGC TGGAGAAATA 
GTGCTCACTG ATTCACCAGA CACACTCATT ATGCATTTCA CCCAAGCCCA GGTAAATCAG 
CATAAAGTTG CCTACAAGCC TCCACAGAAG TTGGGTATTG CACCACGGGT TGTGCAGTTT 
ACCTACCAGG TGGAAGATGC TGCAGGCAAT AGTGTGCCAG GCACATTCAC ATTATTCCTG 
CAACCTGTGG ACAACCAGCC CCCAGAAGTC AACAACAGCG GCTTTACTGT CTTAGAGGGA 
GGCAGCTTTA ACCTCAGCAG TAATGAACTG CATGTTACAG ACCCAGACAC AGACACTGAC 
CAAATTGTCT TCACATTAGT CCAGGGTCCC CAGCATGGAC ACTTACAGTA CTTTAAAAAA 
TGTATGGTCC CAGGGGAATC TTTCATGCAA GCTGATATTA TTAATGGGAG TGTCTCTTAC 
CATCATGACA GAGACCAGAC TACTACTAGG GACACTTTCC ATCTGGAGGT AAGTGATGGG 
GTGCACCTTA TACCCATCAC TGTCCCAATT TCCGTGCACC CCGATGTGGC TAACAAAAGT 
CCTAGGATCT CTATTACAAG TAGTTTCTTA TTGGATGTTT CCATAGATGT ACTAGAGAAT 
AAAGCCACTG AGATTACCAT GGGTGTCATC CATGGAAAAA GGAAGGATGT CCATGACTTG 
ATGCTGTCTT TCATTGTAAA GGACAGCCCC AAATTGGGCA CTATTCTGGT AAATGGTTTG 
TCCAGAGAAC GATTCACCCA AGAAGACCTC ATCAGTGGGA GAGTAGCCTA TGTCCACACT 
GGAGGTGAAG TTGGTTTTCA AAAGCAGCAT GATGCCTTCA GCCTCATCCT CTCCAAGGAT 
TCTTACCAAT GGGTAGTGGG AAATAGCATA GTAGAGAGAA TGCAGGTACA AGTAACTGTG 
CTGCCTGTGG ACAATGTGGG ACCCAAGGTC TTGGTGGGGG AGTCCTTCAT TGTCTATGAA 
GGTGAGAAGA ATCCCTTGAC CTTACAACAT CTCCATATTG AAGATGTGGA CACCCCCCAA 
GATGAACTCC TATGCACAGT GACTAGTCAG CCAGCCTCTG GCTACCTGGA AAAGATTGCA 
GCAACTACTG GTTCAAAAAT GTCCCAGTCT GGTAGCCCCA TCAGTGCTTT CTCCCTCAGA 
GATGTTCAAG TGAGGAATAT CAATTATGTA CAGAGTATTC ACAAGGGAGT AGAGCCACAA 
GAGGATCAAT TCACCTTTTA TTGCTCTGAC GGCATCAACT CCTCCCCAAA TGTCTTCTTC 
CCTATAATCA TCCTCCCTAC CAATGATGAG CAGCCTGAAC TTTCTGTCCA TGAGTTTGTG 
GTACTAGAGG GGATGAGCCT GGTCATAGAC ACCCAGCTGC TCAGTGGAGC AGATGCTGAC 
CTGCCACGGA ATGAGCTGCA CTTTCAACTC ACAGCCCTCC CTCGGCATGG ACGAATCATA 
CAGCAGCTGG ATACAGGTAG CCGGCCCATC CACAGCTTCA CCCTCCAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAT TCAGAGACCA CAGAGGACAG TTTTGAAGTC 
TGGCTGAGTG ACGGCAAGCA CACCACCCAC AGGAAGGTAC CCATTGTAGT GACCCTAGTG 
GATGATGAAA CCCCTCAACT GACTGTCAAC AATGGGCTGA AAGTAGAGAA AGGACAATCT 
GAGATCATCA CAAATCAGAT CCTCAAGGCC ACAGATCTTG ACTCAAATGA TAAGAGCCTC 
AGTTTTGTCC TCCATTCTGG GCCTCAACAA GGTCTTCTAC AGAGACTGAG AGAACCCAGA 
GGAGAAGTGA GAAACAATCT CACTCTGGGA ATGAACTTTA CCCAGGATGA GATTGACAGA 
GGCCTCATCT GCTATACCCA CACAGGCCAA GAAGGGATTG TTGACTTTAT CAAGTTTGAT 
GTGACTGATG GGGCTAACAC TTTGACAGAC CACTGCTTCT ATGTCACCAT TGGCAACTTA 
GACAAAGTCT TCCCTGAGGT AATCAGCAAA AGGGTCACCT TGACAGAAGG TGCCAGAGTG 
ACCCTTACCG ACAGTCTGCT TAGCAACAGT GACGTCAACA GCTCTGATGA ACATCACTTT 
AGCATCACAC GGGCTCCAAG CCTGGGTCAC CTGGAAAGTT CTGACCATGC TGGGGAACCC 
ATTGCCTCTT TCACTCAGCT TCAGTTGACT AGCAACAAAA TATCCTATGT CCATACTTCA 
AATGATGAGA AAAAGATGGA CAGCTTTGAG TTTCAGGTGA TCAGTAAACA CTACCCTGTG 
TTCAGAACCT TCAGGGTCTT CATCACTGAT GTGGATAATA AGAAACCTAT CTTGATCATC 
CATAGACTAA CACTGCAGGA AGAGGATAGC CAACTGATCA CTCCCCTTGA GCTGACAGTG 
GAAGATGAGG ATACTCCAGA TGATCTTATT CTCTTTACCA TCACCCAGGT CCCCAGGCAT 
GGCAAGATTT TGTACAATGG TAACCATCCT GTGACCACTT TCACCAAGCA AGACCTGAAC 
AAGAACCTGA TTAGCTACAA GCATGATGGT AGTGAGACTG CTGAAGACAG TTTCTCCTTG 
ACTGTGACAG ATGGCACTCA CACTGGTTTC TATGTCCTCC CAGACACTGC CCTGGAGACA 
CACAAACCTC AAGTAATGAG GGTCCAGATA AGATCATTAG ATAATAGGCT TCCCCTGATT 
ACTATTAACA GGGGTGCCCC AACCTTGAAA CGCCTTCACA CTGGACACAT GGGCTTCCTG 
ATTACCAGCA AGTCTCTGAA GGCAGAAGAT CAGGACAGTC CCCACAGGCT TCTGAAGTAT 
AAAGTGACCA GAGGACCACA GCATGGCTTC ATCACCAAAA CTGGCCTTGG AAACCAGATC 
ACTCGAGTGT TTACACAAGC TGACATTGAT GAGATGAAGG TCTACTATGT CTTGAATGAG 
GGCAGCAGCA CATCAAAGGA CATCTTCTAT TTCTCTGTTG AAGACAGTGG AGGGAATAAA 
CTAACAAATC AGCCTTTCCG TCTAAACTGG GCTTGGATTT GTTTGGAGAA AGAGTACTAC 
ATCGTGGATG AAGACTCCAC ATTCTTAGAA GTGACCCTTA TACGCAGAGG ACACCTTGGA 
GAAACATCAT TTATCAGCAT TGGTACCAAA GATGAAACTG CAAAAAAAGA TAAAGATTTT 
AAATGGAAGA GCAATAAACA GGTCCAATTC AATCCTGGAC AGACTACAGC CACATTAAGG 
GTGAGAATTA TACCTGACAA TGAATATGAG ACTTCAGAGA CCTTCCAGAT CATTCTGTCA 
GAACCTCTCA TGGCTGCACT GGAGTTTCCA GAAATGGCAA CAGTTGAAAT TGTAGACCCT 
GGAGATGAAT CAACAGTTTA TATTCCAGAG GCGGAATACA AAGTAGAAGA GGATGTTGGG 
GAACTTTTGA TTCCAGTAAG ACGATCTGGA GATGCAAGCC AGGAACTAAT GGTCATTTGC 
TCCACATGTC AAGGTTCTGC CACAGGCACA GTTTCTTCCA CAGTATTATC ATTCTCTGAT 
TACATCTCAC GTCCAGAAGA TCACACCAGC ATCCTCCACT TTGGCAAGAA CGAGACACAG 
AAGACCTGCA GGGTCCTGAT CATCAACGAC TCCCTTTATG AAGAGGAGGA ATTCTTCAGT 
GTTTCACTGA GGCTGCCAGT GGGAGGACAG CTGGGAGCCA GATTCCCCAC AACTAAGGTG 
ACTATCATGG CTGATCGTTA TGATGAACCT GCCCTGCACT TTGGGGATGC TGAATATCAT 
GTCAATGAAA GTGCTGGCTA TGTGGAGATT TGTGTTTGGA GAAGAGGCAC TGATCTTTCC 
CAACCATCAT CCATCACCGT GTTCTCCAGA AAGTCAGAGA AGGAGTCAGC AGAAGCTGGA 
ACAGATTATG TTGGCATCAA CCGAAATCTG GACTTTGCTC CAGGAGTGCG CATGCAGACA 
TTCCGAGTGA CTATCCTTGA TGACATGGGA CAGCCCACCT TGGAAGGCCC AGAGAAGTTT 
GAATTACACC TTCAGATGCC GGTGGGTGCA GTGCTTGGAG AACCAAATAA AACTACCGTT 
TTCATCAAGG ATACCATCAC TGACTGTAAG CTGAGTGCTT GTAGTTCCTT TGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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FREM3 cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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