nca-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	nca-2
Entrez Gene ID	175674
Full Name	Novel Channel type/putative Nematode CAlcium channel
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001136363.2	NP_001129835.1	Novel Channel type/putative Nematode CAlcium channel
NM_001136363.3	NP_001129835.1	Novel Channel type/putative Nematode CAlcium channel
NM_065653.4	NP_498054.2	Novel Channel type/putative Nematode CAlcium channel
NM_065653.5	NP_498054.2	Novel Channel type/putative Nematode CAlcium channel

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NALCN	XP_001149573.1
Canis lupus familiaris (dog)	NALCN	XP_542654.4
Bos taurus (cattle)	NALCN	XP_002692026.1
Rattus norvegicus (Norway rat)	Nalcn	NP_705894.1
Drosophila melanogaster (fruit fly)	na	NP_001096981.1
Homo sapiens (human)	NALCN	NP_443099.1
Caenorhabditis elegans (roundworm)	nca-2	NP_498054.2
Mus musculus (house mouse)	Nalcn	NP_796367.3
Caenorhabditis elegans (roundworm)	unc-77	NP_741413.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003921	XP_318373.5
Gallus gallus (chicken)	NALCN	XP_416967.2
Danio rerio (zebrafish)	nalcn	NP_001017549.2
Xenopus tropicalis (tropical clawed frog)	nalcn	XP_002937125.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nca-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nca-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa341127	NM_065653.5 Latest version!	Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel (nca-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa341131	NM_001136363.3 Latest version!	Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel (nca-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa341131	NM_001136363.2	Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel (nca-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa341127	NM_065653.4	Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel (nca-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nca-2 ( NM_065653.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498054.2 Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel (nca-2), partial mRNA.

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CloneID	OCa341127
Clone ID Related Accession (Same CDS sequence)	NM_065653.4 , NM_065653.5
Accession Version	NM_065653.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554652800000
Organism	Caenorhabditis elegans
Product	Novel Channel type/putative Nematode CAlcium channel
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_065653.3. COMPLETENESS: incomplete on both ends.

ATGACTTCAA CAACTGCAAA ATTGTTGGGA CTGTCAGCGT GCCGTGCGGC CGCATTGTCG 
ACGACAATGC TGACGCGCCG CAAATCGTCA ATAGATGGAA CAAAAACAGA AAGAAATCGG 
AGAAGAGGAG AGTCGATTGG TGGAGCATTT ACTGATATGA TTAATATTGA ACCAACGAGT 
ATTATTAATA AATCGACAGA ATTACTCCAC GAGAGATTTC TCCGAGATAT GGTTCGTGTG 
GCTTGTCTTT TATCAATGAT ATCATTATGT CTTCATACAC CAGAAACTAT CAAAATGTGG 
CCACCACTAA ATTATATAAT TCTGGCGAAT GACGTCATTG TCACTTTGAT TTTTATTGGA 
GAAGCCGCTG TTACGATTAA TCAGAACGGT TTATTCGATA ATCAAAACTC GTATTTACGG 
GATCGATGGT ATCAATTTGA ATTTTTTCTT CTCATTAATC ACATTCTATC ATGTGTTATT 
CATATTTATG AACTTTGCTC AATATGGTTT CCAGCACTTA ATTTTGTGTA TTATCCATGG 
CTTGGAGCAC TTCGATCAGC TCGACCATTC ATTTTTCTTC GATTTATTCG ATCAATAGTG 
AGGTTTAAAC TTCCCAAAAA TCGAATTAAA TTAATCATAA AACGATCATC TCAACAAATC 
CAAAATGTTA CAATATTCTT CATGTTTTTT GTATTTTCGT ATGCAATAAT GGGTGTTCAA 
TTATTTGGAC GACTCAATTA TCACTGCGTT GTCAATGGAA CTGATCCAAA TAATGTTACA 
ATTGCCGATC TAGCAATCCC TGACACAATG TGCTCACAGA AAGGAGCTGG TGGTTACGAG 
TGCCCAGGGA ATATGGTTTG TATGAGATTA CAACTGAAAC CACAAGAAGA AGGATTCTAC 
GGACAATTTA GTGATTTTGC AAGCTCTTTG TTCACAGTTT ATCTGGCTGC ATCTCAAGAA 
GGATGGGTTT ATGTTTTATA TGATTGTTTG GATTCATTAC CTTCATTTCT TGCATTTTTC 
TATTTTGTAA CGCTCATTTT CTTTCTGGCG TGGTTGGTGA AGAATGTTTT CATCGCCGTC 
ATTACCGAAA CATTTGCTGA GATTCGTGTT CAATTCAGTG AAATGTGGCA GACACGTGAA 
GCAACAACTG ATCATGTGTA CACACAGAAA CTGGAAAAAG ATGAAGATGG ATGGAAATTG 
GTGGAAGTTG ATAAATACAA TAGAGCTCAT TCTAATAATT CACTTTTCTT GCATACAATA 
GTCACTTCAA CAGCTTTTCA AACAGTCATG CAATTGTTAA TCCTTGCAAA TGCAATATTT 
CATGCTACTT TTGTATTTTA TCATGATGAA TCCGATCAAA TCAGGAAAAT TTGGTATTAT 
TACGTGGAAG TTGGCTTTAC GATACTCTTT AATACGGAAG TCATCATAAA GATTTATGCA 
TTTGGATGGA AAGCTTATAT TGCACGTGGG CAACATAAAT TTGATTGTAT TCTTTGCGTT 
GGTTCAAGCC TCAATGCAAT ATGGGTTCTT TATGAAACAA ATATCTTCAC ATACTTCCAA 
GTTTTCCGAA TTGCTCGACT TATCAAAGCC TCTCCAATGC TAGAAGACTT CGTTTATAAA 
ATCTTTGGAC CCGGAAAGAA ATTGGGAGGT CTTGTGATAT TCACTGGAAT TCTTTTGATC 
GTTACTTCTG CAATTTCGTT GCAGCTATTT TGCTATGTGC CAAAATTAAA CAAATTTACC 
AATTTTGCTG TGGCTTTCAT GTCAATGTTC CAAATAATAA CACAAGAAGG CTGGACAGAT 
GTTGTCATTG AAATTCTAAG AGCATGTAAT GAACAAGCGG TTCCATTTGT TGCCATCTAT 
TTTGTTGCTT ATCACTTACT GGTTACACTG TTCGTTTTGT CACTCTTCGT TGCCGTCATT 
CTTGATAATT TGGAAATGGA CGAAGAATTG AAAAAAGTGA AACAACTGAA AGCAAGAGAA 
CAGGATACAA TAAAGACAAC ACTTCCGATG CGTCTCCGCA TTTTCAATCG ATTTCCGACT 
GCTCCAACAA TGGTTACAAT GAAGAAAGTA TCGTCAGAGT TTCCTCTTCC AAAAATTAGA 
GATTCGTTTA CAAGACAGTT TGCGGATGAA TTCGTAGAGA CAAGTGATGA TACTATTCAG 
GAAATCGGAT TTAAAGTCCG ATCAATGTTA TCCGGTAGAG GACCATCTAA AGAAACACGT 
ATAACAACAA CAATCCGACA TGTTGGACAA CTTTCAAACA AAACAATTCT CACCTCAATG 
CTCACTGAAT CAAATCGGAA TCGTGCTCTT TTCTCGGAAT CAAATCAACA TTTGGCTAGT 
TTAACCAGAA GTAACACTTC ATCAAAACAC GGAAAAAGTG GAGCTTTATC AACGAATTCT 
CGATCAAGAA CACGTGGATT GGCTTCATTA AAAGGGAAAC ATATGGTGGA AGGATTCAAA 
GAAAATGGAG ATTTACGACC AGAAGACACT GCTAGAAAAG TGGAGAAGCA TGGGGAGATT 
GATTTTAAAG CATTGCAAAT GAAAAGGGCT CATGCTGAAA TTACAAGAAA CCGAATAGAA 
GAAGAAATGC GCGAGAACCA TCCGATGTTC GATCGTCCGC TCTTTCTTGT TGGCAGAGAA 
TCGTCTCTAC GTAGAATGTG CCAACTCATT GCTCATTCCA GACATTCATA CGATCAAAAT 
GATGGCCAAC ACAGAAAACA TTCGAATAAA TATAAACAAT TTCATGACTT TCTGGCCATC 
ATGACATATA TGGATTGGAC AATGGTGCTA GTAACAACCT TATCATGTTG CAGTATGTTA 
TGGGAATCAC CTTGGCCAAC GACAGGAGAG AATCTGATTT TTAATAACTT TTATCTCCAA 
ATTGCTGAAT ACATCTTTGT GCTTGTGATG AGTTTCGAAC TAATTGTAAA ATGCATTGCA 
AATGGTCTCT TCTTCACACC AAAAGCCTTG GTAACAGATG TCGGAGATAT TCTTACGATT 
TTCATTTATA TTACAAGTCT AATGTTTCTG ATATGGATGC CAAATCACAT CGAAATCAAC 
AGCTGGGCTC AGCTTCTTAT GGTTTGCCGA GCAATGAGAC CATTGAGAGT TTACGCGTTG 
ATTCCGCATA TTCGAAGAGT CGTTGTGGAG TTATGCAGAG GATTTCGGGA AATTTTATTG 
GTAACAATTC TTCTCGTCGT TCTCATGTTC ATTTTCGCAA GTTTCGGAGT TCAACTAGTC 
GGTGGAAAGC TAGCATCATG CAATGATCCA ATGATAACAT CTCGTGAAAA CTGTACAGGA 
TTGTATGACG TCAAATTGTT TGTTACTCGG ATGGAAGTTT ATGGTAAAAA TGACAATCTG 
ATGCATCCTT CAATCATTGT GCCACGTGTA TGGACAAATC CACGGAATTT CAATTTCGAT 
CACATTGGGA ATGCAATGTT GGCTCTTTTT GAAACACTTT CCTACAAGGG ATGGAATGTA 
GTCAGAGATG TTTTATACTT GCGACATGGA GCTTGGGCAG TTCTCTTCAT TCACATTTAT 
GTATTCATTG GATGTATGAT TGGACTAACA CTATTCGTCG GTGTGGTTGT TGCAAATTAC 
ACTGAAAACC GTGGAACAGC CCTTCTAACA GTTGATCAAC GTCGTTGGCA TGATCTGAAG 
GCTCGTCTGA AAATGGCTCA ACCTCTACAT GTTCCACCGA AACCTCCGGA ATCTGCAAAA 
CTGCGTTGTT ACCTCTATGA TCTGACGACG TCTCGATGGT TCAAACAGTT ATTTGCTGTT 
CTTGTGGTTG TCAATTCATT TACACTTGTT ATTCCATGGA ATGTTAGTGA AGAACAGGAT 
AGAAAGACTT TTCTGTTATG TCTTACTGTC ATTTCTGCAA TTTGCAATAT TTTATTCACT 
TTGGAATGCT TGCTGAAAAT GATTGCGTTC ACGTTATCGG GATTTTGGCA ATCAAGAAGA 
AATCGAATTG ATTTTATTAT CACTATCCTC GGAATCAATT GGATTGTTTT TCATTTTTTG 
TTTCAATTAC CTGCGTACTT TGCTGGTGGA ATAACCGAAT GGAAGCGTCT CACATACACT 
TATGGATATC TCGTCGTTAT ACTCCGTTTT TTCACAATTG CCGGCCGTAA ATCCACACTG 
AAAATGTTAA TGTTAACAGT TGTAATGTCA ATGGTTCGAT CATCATTTAT TATTGCTGCA 
ATGTTTCTAT TGGTTCTTTT TTATGCAAAT GCAGGAGTTG TGTTATTTGG AATGGTCAAA 
TATGGACAAG CTGTTGGAAA ACATGTAAAT TTCCGAAATG GACGAGAGGC ATTGGTGGTG 
TTATTTCGAT CAGTTACCGG TGAAGATTGG AATGATATTA TGCACGATTG TATGAGAGCA 
CCACCATTTT GCAATTGGCA TCCAGGGCTC AGCTATTGGC AAACTGATTG TGGGAATTAT 
GTTGGAGCAA TTGTTTATTT TTGCTCCTTC TATCTCATTA TCACATATAT TGTGCTCAAT 
CTTCTTGTCG CTATCATCAT GGAGAACTTC TCATTGTTCT ACTCATCTGA AGAAGATGCA 
CTTCTCTCGT ATGCCGATAT TCGAAATTTC CAACTTGTTT GGAATATGGT GGATATTGAA 
CAGAAACGAT CAATTCCAGT GAGACGTGTC AAATTTCTGT TGAGATTGTT AAAAGGAAGA 
CTGGAAGTTA ATGATGAATC CGATGGATTG TTGTTTAAAC ATATGTGTCA TGAAATGGAA 
CGATTGCATA ATGGAGATGA TGTTTCATTT CATGATGTTC TCAACATGCT CTCGTATCGA 
AGTGTGGATA TTCGAAAAAG TTTACAACTG GAAGAATTAC TTCAAAGAGA AGAACTTGAA 
TACATAATTG AAGAAGAAGT TGCAAAGCAC ACTATTCGGG CGTGGCTAGA GAATTGTTTG 
AAAAACATCA AAGCCAAGCA GAATAATACT CTTGGAAAAA TGAGCTCAAT TGGGTCAACA 
TTTGCATTTC CACAATCTCA AGAAGTATTA ACAAAAGGAG TTGTGCTCAC GGAGGCTTCT 
CCTGAAGAAG ATTCATTGCA AGGAGATAAG GGCAGTGGAA AGAAGAAGGC TCAGAGAGGC 
AACTCTATTA CAGAGATAGT GGCAGAAGCG CAAAAGAAAA GTGTAAAACG AGCAACTGAT 
AAAATAAGTG AACGTCGTGG AACACTTCGT CAAATGCAAA TGGGAACATA TGATGAATTG 
GAAGAAGTTG AAGAAGACGA AGATACTGAC GGAGAAATCA GAAGATCATC ATTTGAATAT 
TCAGGAGTTG ATATGATTCA AATGTCACAT GAAAAACAAC TGGAAGATGT GAAAACTTGG 
TGGACGTTGT GTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498054.2
CDS	1..5358
Translation	MTSTTAKLLGLSACRAAALSTTMLTRRKSSIDGTKTERNRRRGESIGGAFTDMINIEPTSIINKSTELLHERFLRDMVRVACLLSMISLCLHTPETIKMWPPLNYIILANDVIVTLIFIGEAAVTINQNGLFDNQNSYLRDRWYQFEFFLLINHILSCVIHIYELCSIWFPALNFVYYPWLGALRSARPFIFLRFIRSIVRFKLPKNRIKLIIKRSSQQIQNVTIFFMFFVFSYAIMGVQLFGRLNYHCVVNGTDPNNVTIADLAIPDTMCSQKGAGGYECPGNMVCMRLQLKPQEEGFYGQFSDFASSLFTVYLAASQEGWVYVLYDCLDSLPSFLAFFYFVTLIFFLAWLVKNVFIAVITETFAEIRVQFSEMWQTREATTDHVYTQKLEKDEDGWKLVEVDKYNRAHSNNSLFLHTIVTSTAFQTVMQLLILANAIFHATFVFYHDESDQIRKIWYYYVEVGFTILFNTEVIIKIYAFGWKAYIARGQHKFDCILCVGSSLNAIWVLYETNIFTYFQVFRIARLIKASPMLEDFVYKIFGPGKKLGGLVIFTGILLIVTSAISLQLFCYVPKLNKFTNFAVAFMSMFQIITQEGWTDVVIEILRACNEQAVPFVAIYFVAYHLLVTLFVLSLFVAVILDNLEMDEELKKVKQLKAREQDTIKTTLPMRLRIFNRFPTAPTMVTMKKVSSEFPLPKIRDSFTRQFADEFVETSDDTIQEIGFKVRSMLSGRGPSKETRITTTIRHVGQLSNKTILTSMLTESNRNRALFSESNQHLASLTRSNTSSKHGKSGALSTNSRSRTRGLASLKGKHMVEGFKENGDLRPEDTARKVEKHGEIDFKALQMKRAHAEITRNRIEEEMRENHPMFDRPLFLVGRESSLRRMCQLIAHSRHSYDQNDGQHRKHSNKYKQFHDFLAIMTYMDWTMVLVTTLSCCSMLWESPWPTTGENLIFNNFYLQIAEYIFVLVMSFELIVKCIANGLFFTPKALVTDVGDILTIFIYITSLMFLIWMPNHIEINSWAQLLMVCRAMRPLRVYALIPHIRRVVVELCRGFREILLVTILLVVLMFIFASFGVQLVGGKLASCNDPMITSRENCTGLYDVKLFVTRMEVYGKNDNLMHPSIIVPRVWTNPRNFNFDHIGNAMLALFETLSYKGWNVVRDVLYLRHGAWAVLFIHIYVFIGCMIGLTLFVGVVVANYTENRGTALLTVDQRRWHDLKARLKMAQPLHVPPKPPESAKLRCYLYDLTTSRWFKQLFAVLVVVNSFTLVIPWNVSEEQDRKTFLLCLTVISAICNILFTLECLLKMIAFTLSGFWQSRRNRIDFIITILGINWIVFHFLFQLPAYFAGGITEWKRLTYTYGYLVVILRFFTIAGRKSTLKMLMLTVVMSMVRSSFIIAAMFLLVLFYANAGVVLFGMVKYGQAVGKHVNFRNGREALVVLFRSVTGEDWNDIMHDCMRAPPFCNWHPGLSYWQTDCGNYVGAIVYFCSFYLIITYIVLNLLVAIIMENFSLFYSSEEDALLSYADIRNFQLVWNMVDIEQKRSIPVRRVKFLLRLLKGRLEVNDESDGLLFKHMCHEMERLHNGDDVSFHDVLNMLSYRSVDIRKSLQLEELLQREELEYIIEEEVAKHTIRAWLENCLKNIKAKQNNTLGKMSSIGSTFAFPQSQEVLTKGVVLTEASPEEDSLQGDKGSGKKKAQRGNSITEIVAEAQKKSVKRATDKISERRGTLRQMQMGTYDELEEVEEDEDTDGEIRRSSFEYSGVDMIQMSHEKQLEDVKTWWTLCD

Target ORF information:

RefSeq Version	NM_065653.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel (nca-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065653.4

ORF Insert Sequence:

ATGACTTCAA CAACTGCAAA ATTGTTGGGA CTGTCAGCGT GCCGTGCGGC CGCATTGTCG 
ACGACAATGC TGACGCGCCG CAAATCGTCA ATAGATGGAA CAAAAACAGA AAGAAATCGG 
AGAAGAGGAG AGTCGATTGG TGGAGCATTT ACTGATATGA TTAATATTGA ACCAACGAGT 
ATTATTAATA AATCGACAGA ATTACTCCAC GAGAGATTTC TCCGAGATAT GGTTCGTGTG 
GCTTGTCTTT TATCAATGAT ATCATTATGT CTTCATACAC CAGAAACTAT CAAAATGTGG 
CCACCACTAA ATTATATAAT TCTGGCGAAT GACGTCATTG TCACTTTGAT TTTTATTGGA 
GAAGCCGCTG TTACGATTAA TCAGAACGGT TTATTCGATA ATCAAAACTC GTATTTACGG 
GATCGATGGT ATCAATTTGA ATTTTTTCTT CTCATTAATC ACATTCTATC ATGTGTTATT 
CATATTTATG AACTTTGCTC AATATGGTTT CCAGCACTTA ATTTTGTGTA TTATCCATGG 
CTTGGAGCAC TTCGATCAGC TCGACCATTC ATTTTTCTTC GATTTATTCG ATCAATAGTG 
AGGTTTAAAC TTCCCAAAAA TCGAATTAAA TTAATCATAA AACGATCATC TCAACAAATC 
CAAAATGTTA CAATATTCTT CATGTTTTTT GTATTTTCGT ATGCAATAAT GGGTGTTCAA 
TTATTTGGAC GACTCAATTA TCACTGCGTT GTCAATGGAA CTGATCCAAA TAATGTTACA 
ATTGCCGATC TAGCAATCCC TGACACAATG TGCTCACAGA AAGGAGCTGG TGGTTACGAG 
TGCCCAGGGA ATATGGTTTG TATGAGATTA CAACTGAAAC CACAAGAAGA AGGATTCTAC 
GGACAATTTA GTGATTTTGC AAGCTCTTTG TTCACAGTTT ATCTGGCTGC ATCTCAAGAA 
GGATGGGTTT ATGTTTTATA TGATTGTTTG GATTCATTAC CTTCATTTCT TGCATTTTTC 
TATTTTGTAA CGCTCATTTT CTTTCTGGCG TGGTTGGTGA AGAATGTTTT CATCGCCGTC 
ATTACCGAAA CATTTGCTGA GATTCGTGTT CAATTCAGTG AAATGTGGCA GACACGTGAA 
GCAACAACTG ATCATGTGTA CACACAGAAA CTGGAAAAAG ATGAAGATGG ATGGAAATTG 
GTGGAAGTTG ATAAATACAA TAGAGCTCAT TCTAATAATT CACTTTTCTT GCATACAATA 
GTCACTTCAA CAGCTTTTCA AACAGTCATG CAATTGTTAA TCCTTGCAAA TGCAATATTT 
CATGCTACTT TTGTATTTTA TCATGATGAA TCCGATCAAA TCAGGAAAAT TTGGTATTAT 
TACGTGGAAG TTGGCTTTAC GATACTCTTT AATACGGAAG TCATCATAAA GATTTATGCA 
TTTGGATGGA AAGCTTATAT TGCACGTGGG CAACATAAAT TTGATTGTAT TCTTTGCGTT 
GGTTCAAGCC TCAATGCAAT ATGGGTTCTT TATGAAACAA ATATCTTCAC ATACTTCCAA 
GTTTTCCGAA TTGCTCGACT TATCAAAGCC TCTCCAATGC TAGAAGACTT CGTTTATAAA 
ATCTTTGGAC CCGGAAAGAA ATTGGGAGGT CTTGTGATAT TCACTGGAAT TCTTTTGATC 
GTTACTTCTG CAATTTCGTT GCAGCTATTT TGCTATGTGC CAAAATTAAA CAAATTTACC 
AATTTTGCTG TGGCTTTCAT GTCAATGTTC CAAATAATAA CACAAGAAGG CTGGACAGAT 
GTTGTCATTG AAATTCTAAG AGCATGTAAT GAACAAGCGG TTCCATTTGT TGCCATCTAT 
TTTGTTGCTT ATCACTTACT GGTTACACTG TTCGTTTTGT CACTCTTCGT TGCCGTCATT 
CTTGATAATT TGGAAATGGA CGAAGAATTG AAAAAAGTGA AACAACTGAA AGCAAGAGAA 
CAGGATACAA TAAAGACAAC ACTTCCGATG CGTCTCCGCA TTTTCAATCG ATTTCCGACT 
GCTCCAACAA TGGTTACAAT GAAGAAAGTA TCGTCAGAGT TTCCTCTTCC AAAAATTAGA 
GATTCGTTTA CAAGACAGTT TGCGGATGAA TTCGTAGAGA CAAGTGATGA TACTATTCAG 
GAAATCGGAT TTAAAGTCCG ATCAATGTTA TCCGGTAGAG GACCATCTAA AGAAACACGT 
ATAACAACAA CAATCCGACA TGTTGGACAA CTTTCAAACA AAACAATTCT CACCTCAATG 
CTCACTGAAT CAAATCGGAA TCGTGCTCTT TTCTCGGAAT CAAATCAACA TTTGGCTAGT 
TTAACCAGAA GTAACACTTC ATCAAAACAC GGAAAAAGTG GAGCTTTATC AACGAATTCT 
CGATCAAGAA CACGTGGATT GGCTTCATTA AAAGGGAAAC ATATGGTGGA AGGATTCAAA 
GAAAATGGAG ATTTACGACC AGAAGACACT GCTAGAAAAG TGGAGAAGCA TGGGGAGATT 
GATTTTAAAG CATTGCAAAT GAAAAGGGCT CATGCTGAAA TTACAAGAAA CCGAATAGAA 
GAAGAAATGC GCGAGAACCA TCCGATGTTC GATCGTCCGC TCTTTCTTGT TGGCAGAGAA 
TCGTCTCTAC GTAGAATGTG CCAACTCATT GCTCATTCCA GACATTCATA CGATCAAAAT 
GATGGCCAAC ACAGAAAACA TTCGAATAAA TATAAACAAT TTCATGACTT TCTGGCCATC 
ATGACATATA TGGATTGGAC AATGGTGCTA GTAACAACCT TATCATGTTG CAGTATGTTA 
TGGGAATCAC CTTGGCCAAC GACAGGAGAG AATCTGATTT TTAATAACTT TTATCTCCAA 
ATTGCTGAAT ACATCTTTGT GCTTGTGATG AGTTTCGAAC TAATTGTAAA ATGCATTGCA 
AATGGTCTCT TCTTCACACC AAAAGCCTTG GTAACAGATG TCGGAGATAT TCTTACGATT 
TTCATTTATA TTACAAGTCT AATGTTTCTG ATATGGATGC CAAATCACAT CGAAATCAAC 
AGCTGGGCTC AGCTTCTTAT GGTTTGCCGA GCAATGAGAC CATTGAGAGT TTACGCGTTG 
ATTCCGCATA TTCGAAGAGT CGTTGTGGAG TTATGCAGAG GATTTCGGGA AATTTTATTG 
GTAACAATTC TTCTCGTCGT TCTCATGTTC ATTTTCGCAA GTTTCGGAGT TCAACTAGTC 
GGTGGAAAGC TAGCATCATG CAATGATCCA ATGATAACAT CTCGTGAAAA CTGTACAGGA 
TTGTATGACG TCAAATTGTT TGTTACTCGG ATGGAAGTTT ATGGTAAAAA TGACAATCTG 
ATGCATCCTT CAATCATTGT GCCACGTGTA TGGACAAATC CACGGAATTT CAATTTCGAT 
CACATTGGGA ATGCAATGTT GGCTCTTTTT GAAACACTTT CCTACAAGGG ATGGAATGTA 
GTCAGAGATG TTTTATACTT GCGACATGGA GCTTGGGCAG TTCTCTTCAT TCACATTTAT 
GTATTCATTG GATGTATGAT TGGACTAACA CTATTCGTCG GTGTGGTTGT TGCAAATTAC 
ACTGAAAACC GTGGAACAGC CCTTCTAACA GTTGATCAAC GTCGTTGGCA TGATCTGAAG 
GCTCGTCTGA AAATGGCTCA ACCTCTACAT GTTCCACCGA AACCTCCGGA ATCTGCAAAA 
CTGCGTTGTT ACCTCTATGA TCTGACGACG TCTCGATGGT TCAAACAGTT ATTTGCTGTT 
CTTGTGGTTG TCAATTCATT TACACTTGTT ATTCCATGGA ATGTTAGTGA AGAACAGGAT 
AGAAAGACTT TTCTGTTATG TCTTACTGTC ATTTCTGCAA TTTGCAATAT TTTATTCACT 
TTGGAATGCT TGCTGAAAAT GATTGCGTTC ACGTTATCGG GATTTTGGCA ATCAAGAAGA 
AATCGAATTG ATTTTATTAT CACTATCCTC GGAATCAATT GGATTGTTTT TCATTTTTTG 
TTTCAATTAC CTGCGTACTT TGCTGGTGGA ATAACCGAAT GGAAGCGTCT CACATACACT 
TATGGATATC TCGTCGTTAT ACTCCGTTTT TTCACAATTG CCGGCCGTAA ATCCACACTG 
AAAATGTTAA TGTTAACAGT TGTAATGTCA ATGGTTCGAT CATCATTTAT TATTGCTGCA 
ATGTTTCTAT TGGTTCTTTT TTATGCAAAT GCAGGAGTTG TGTTATTTGG AATGGTCAAA 
TATGGACAAG CTGTTGGAAA ACATGTAAAT TTCCGAAATG GACGAGAGGC ATTGGTGGTG 
TTATTTCGAT CAGTTACCGG TGAAGATTGG AATGATATTA TGCACGATTG TATGAGAGCA 
CCACCATTTT GCAATTGGCA TCCAGGGCTC AGCTATTGGC AAACTGATTG TGGGAATTAT 
GTTGGAGCAA TTGTTTATTT TTGCTCCTTC TATCTCATTA TCACATATAT TGTGCTCAAT 
CTTCTTGTCG CTATCATCAT GGAGAACTTC TCATTGTTCT ACTCATCTGA AGAAGATGCA 
CTTCTCTCGT ATGCCGATAT TCGAAATTTC CAACTTGTTT GGAATATGGT GGATATTGAA 
CAGAAACGAT CAATTCCAGT GAGACGTGTC AAATTTCTGT TGAGATTGTT AAAAGGAAGA 
CTGGAAGTTA ATGATGAATC CGATGGATTG TTGTTTAAAC ATATGTGTCA TGAAATGGAA 
CGATTGCATA ATGGAGATGA TGTTTCATTT CATGATGTTC TCAACATGCT CTCGTATCGA 
AGTGTGGATA TTCGAAAAAG TTTACAACTG GAAGAATTAC TTCAAAGAGA AGAACTTGAA 
TACATAATTG AAGAAGAAGT TGCAAAGCAC ACTATTCGGG CGTGGCTAGA GAATTGTTTG 
AAAAACATCA AAGCCAAGCA GAATAATACT CTTGGAAAAA TGAGCTCAAT TGGGTCAACA 
TTTGCATTTC CACAATCTCA AGAAGTATTA ACAAAAGGAG TTGTGCTCAC GGAGGCTTCT 
CCTGAAGAAG ATTCATTGCA AGGAGATAAG GGCAGTGGAA AGAAGAAGGC TCAGAGAGGC 
AACTCTATTA CAGAGATAGT GGCAGAAGCG CAAAAGAAAA GTGTAAAACG AGCAACTGAT 
AAAATAAGTG AACGTCGTGG AACACTTCGT CAAATGCAAA TGGGAACATA TGATGAATTG 
GAAGAAGTTG AAGAAGACGA AGATACTGAC GGAGAAATCA GAAGATCATC ATTTGAATAT 
TCAGGAGTTG ATATGATTCA AATGTCACAT GAAAAACAAC TGGAAGATGT GAAAACTTGG 
TGGACGTTGT GTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nca-2 ( NM_065653.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498054.2 Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel (nca-2), mRNA.

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CloneID	OCa341127
Clone ID Related Accession (Same CDS sequence)	NM_065653.4 , NM_065653.5
Accession Version	NM_065653.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571241600000
Organism	Caenorhabditis elegans
Product	Novel Channel type/putative Nematode CAlcium channel
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_065653.4.

ATGACTTCAA CAACTGCAAA ATTGTTGGGA CTGTCAGCGT GCCGTGCGGC CGCATTGTCG 
ACGACAATGC TGACGCGCCG CAAATCGTCA ATAGATGGAA CAAAAACAGA AAGAAATCGG 
AGAAGAGGAG AGTCGATTGG TGGAGCATTT ACTGATATGA TTAATATTGA ACCAACGAGT 
ATTATTAATA AATCGACAGA ATTACTCCAC GAGAGATTTC TCCGAGATAT GGTTCGTGTG 
GCTTGTCTTT TATCAATGAT ATCATTATGT CTTCATACAC CAGAAACTAT CAAAATGTGG 
CCACCACTAA ATTATATAAT TCTGGCGAAT GACGTCATTG TCACTTTGAT TTTTATTGGA 
GAAGCCGCTG TTACGATTAA TCAGAACGGT TTATTCGATA ATCAAAACTC GTATTTACGG 
GATCGATGGT ATCAATTTGA ATTTTTTCTT CTCATTAATC ACATTCTATC ATGTGTTATT 
CATATTTATG AACTTTGCTC AATATGGTTT CCAGCACTTA ATTTTGTGTA TTATCCATGG 
CTTGGAGCAC TTCGATCAGC TCGACCATTC ATTTTTCTTC GATTTATTCG ATCAATAGTG 
AGGTTTAAAC TTCCCAAAAA TCGAATTAAA TTAATCATAA AACGATCATC TCAACAAATC 
CAAAATGTTA CAATATTCTT CATGTTTTTT GTATTTTCGT ATGCAATAAT GGGTGTTCAA 
TTATTTGGAC GACTCAATTA TCACTGCGTT GTCAATGGAA CTGATCCAAA TAATGTTACA 
ATTGCCGATC TAGCAATCCC TGACACAATG TGCTCACAGA AAGGAGCTGG TGGTTACGAG 
TGCCCAGGGA ATATGGTTTG TATGAGATTA CAACTGAAAC CACAAGAAGA AGGATTCTAC 
GGACAATTTA GTGATTTTGC AAGCTCTTTG TTCACAGTTT ATCTGGCTGC ATCTCAAGAA 
GGATGGGTTT ATGTTTTATA TGATTGTTTG GATTCATTAC CTTCATTTCT TGCATTTTTC 
TATTTTGTAA CGCTCATTTT CTTTCTGGCG TGGTTGGTGA AGAATGTTTT CATCGCCGTC 
ATTACCGAAA CATTTGCTGA GATTCGTGTT CAATTCAGTG AAATGTGGCA GACACGTGAA 
GCAACAACTG ATCATGTGTA CACACAGAAA CTGGAAAAAG ATGAAGATGG ATGGAAATTG 
GTGGAAGTTG ATAAATACAA TAGAGCTCAT TCTAATAATT CACTTTTCTT GCATACAATA 
GTCACTTCAA CAGCTTTTCA AACAGTCATG CAATTGTTAA TCCTTGCAAA TGCAATATTT 
CATGCTACTT TTGTATTTTA TCATGATGAA TCCGATCAAA TCAGGAAAAT TTGGTATTAT 
TACGTGGAAG TTGGCTTTAC GATACTCTTT AATACGGAAG TCATCATAAA GATTTATGCA 
TTTGGATGGA AAGCTTATAT TGCACGTGGG CAACATAAAT TTGATTGTAT TCTTTGCGTT 
GGTTCAAGCC TCAATGCAAT ATGGGTTCTT TATGAAACAA ATATCTTCAC ATACTTCCAA 
GTTTTCCGAA TTGCTCGACT TATCAAAGCC TCTCCAATGC TAGAAGACTT CGTTTATAAA 
ATCTTTGGAC CCGGAAAGAA ATTGGGAGGT CTTGTGATAT TCACTGGAAT TCTTTTGATC 
GTTACTTCTG CAATTTCGTT GCAGCTATTT TGCTATGTGC CAAAATTAAA CAAATTTACC 
AATTTTGCTG TGGCTTTCAT GTCAATGTTC CAAATAATAA CACAAGAAGG CTGGACAGAT 
GTTGTCATTG AAATTCTAAG AGCATGTAAT GAACAAGCGG TTCCATTTGT TGCCATCTAT 
TTTGTTGCTT ATCACTTACT GGTTACACTG TTCGTTTTGT CACTCTTCGT TGCCGTCATT 
CTTGATAATT TGGAAATGGA CGAAGAATTG AAAAAAGTGA AACAACTGAA AGCAAGAGAA 
CAGGATACAA TAAAGACAAC ACTTCCGATG CGTCTCCGCA TTTTCAATCG ATTTCCGACT 
GCTCCAACAA TGGTTACAAT GAAGAAAGTA TCGTCAGAGT TTCCTCTTCC AAAAATTAGA 
GATTCGTTTA CAAGACAGTT TGCGGATGAA TTCGTAGAGA CAAGTGATGA TACTATTCAG 
GAAATCGGAT TTAAAGTCCG ATCAATGTTA TCCGGTAGAG GACCATCTAA AGAAACACGT 
ATAACAACAA CAATCCGACA TGTTGGACAA CTTTCAAACA AAACAATTCT CACCTCAATG 
CTCACTGAAT CAAATCGGAA TCGTGCTCTT TTCTCGGAAT CAAATCAACA TTTGGCTAGT 
TTAACCAGAA GTAACACTTC ATCAAAACAC GGAAAAAGTG GAGCTTTATC AACGAATTCT 
CGATCAAGAA CACGTGGATT GGCTTCATTA AAAGGGAAAC ATATGGTGGA AGGATTCAAA 
GAAAATGGAG ATTTACGACC AGAAGACACT GCTAGAAAAG TGGAGAAGCA TGGGGAGATT 
GATTTTAAAG CATTGCAAAT GAAAAGGGCT CATGCTGAAA TTACAAGAAA CCGAATAGAA 
GAAGAAATGC GCGAGAACCA TCCGATGTTC GATCGTCCGC TCTTTCTTGT TGGCAGAGAA 
TCGTCTCTAC GTAGAATGTG CCAACTCATT GCTCATTCCA GACATTCATA CGATCAAAAT 
GATGGCCAAC ACAGAAAACA TTCGAATAAA TATAAACAAT TTCATGACTT TCTGGCCATC 
ATGACATATA TGGATTGGAC AATGGTGCTA GTAACAACCT TATCATGTTG CAGTATGTTA 
TGGGAATCAC CTTGGCCAAC GACAGGAGAG AATCTGATTT TTAATAACTT TTATCTCCAA 
ATTGCTGAAT ACATCTTTGT GCTTGTGATG AGTTTCGAAC TAATTGTAAA ATGCATTGCA 
AATGGTCTCT TCTTCACACC AAAAGCCTTG GTAACAGATG TCGGAGATAT TCTTACGATT 
TTCATTTATA TTACAAGTCT AATGTTTCTG ATATGGATGC CAAATCACAT CGAAATCAAC 
AGCTGGGCTC AGCTTCTTAT GGTTTGCCGA GCAATGAGAC CATTGAGAGT TTACGCGTTG 
ATTCCGCATA TTCGAAGAGT CGTTGTGGAG TTATGCAGAG GATTTCGGGA AATTTTATTG 
GTAACAATTC TTCTCGTCGT TCTCATGTTC ATTTTCGCAA GTTTCGGAGT TCAACTAGTC 
GGTGGAAAGC TAGCATCATG CAATGATCCA ATGATAACAT CTCGTGAAAA CTGTACAGGA 
TTGTATGACG TCAAATTGTT TGTTACTCGG ATGGAAGTTT ATGGTAAAAA TGACAATCTG 
ATGCATCCTT CAATCATTGT GCCACGTGTA TGGACAAATC CACGGAATTT CAATTTCGAT 
CACATTGGGA ATGCAATGTT GGCTCTTTTT GAAACACTTT CCTACAAGGG ATGGAATGTA 
GTCAGAGATG TTTTATACTT GCGACATGGA GCTTGGGCAG TTCTCTTCAT TCACATTTAT 
GTATTCATTG GATGTATGAT TGGACTAACA CTATTCGTCG GTGTGGTTGT TGCAAATTAC 
ACTGAAAACC GTGGAACAGC CCTTCTAACA GTTGATCAAC GTCGTTGGCA TGATCTGAAG 
GCTCGTCTGA AAATGGCTCA ACCTCTACAT GTTCCACCGA AACCTCCGGA ATCTGCAAAA 
CTGCGTTGTT ACCTCTATGA TCTGACGACG TCTCGATGGT TCAAACAGTT ATTTGCTGTT 
CTTGTGGTTG TCAATTCATT TACACTTGTT ATTCCATGGA ATGTTAGTGA AGAACAGGAT 
AGAAAGACTT TTCTGTTATG TCTTACTGTC ATTTCTGCAA TTTGCAATAT TTTATTCACT 
TTGGAATGCT TGCTGAAAAT GATTGCGTTC ACGTTATCGG GATTTTGGCA ATCAAGAAGA 
AATCGAATTG ATTTTATTAT CACTATCCTC GGAATCAATT GGATTGTTTT TCATTTTTTG 
TTTCAATTAC CTGCGTACTT TGCTGGTGGA ATAACCGAAT GGAAGCGTCT CACATACACT 
TATGGATATC TCGTCGTTAT ACTCCGTTTT TTCACAATTG CCGGCCGTAA ATCCACACTG 
AAAATGTTAA TGTTAACAGT TGTAATGTCA ATGGTTCGAT CATCATTTAT TATTGCTGCA 
ATGTTTCTAT TGGTTCTTTT TTATGCAAAT GCAGGAGTTG TGTTATTTGG AATGGTCAAA 
TATGGACAAG CTGTTGGAAA ACATGTAAAT TTCCGAAATG GACGAGAGGC ATTGGTGGTG 
TTATTTCGAT CAGTTACCGG TGAAGATTGG AATGATATTA TGCACGATTG TATGAGAGCA 
CCACCATTTT GCAATTGGCA TCCAGGGCTC AGCTATTGGC AAACTGATTG TGGGAATTAT 
GTTGGAGCAA TTGTTTATTT TTGCTCCTTC TATCTCATTA TCACATATAT TGTGCTCAAT 
CTTCTTGTCG CTATCATCAT GGAGAACTTC TCATTGTTCT ACTCATCTGA AGAAGATGCA 
CTTCTCTCGT ATGCCGATAT TCGAAATTTC CAACTTGTTT GGAATATGGT GGATATTGAA 
CAGAAACGAT CAATTCCAGT GAGACGTGTC AAATTTCTGT TGAGATTGTT AAAAGGAAGA 
CTGGAAGTTA ATGATGAATC CGATGGATTG TTGTTTAAAC ATATGTGTCA TGAAATGGAA 
CGATTGCATA ATGGAGATGA TGTTTCATTT CATGATGTTC TCAACATGCT CTCGTATCGA 
AGTGTGGATA TTCGAAAAAG TTTACAACTG GAAGAATTAC TTCAAAGAGA AGAACTTGAA 
TACATAATTG AAGAAGAAGT TGCAAAGCAC ACTATTCGGG CGTGGCTAGA GAATTGTTTG 
AAAAACATCA AAGCCAAGCA GAATAATACT CTTGGAAAAA TGAGCTCAAT TGGGTCAACA 
TTTGCATTTC CACAATCTCA AGAAGTATTA ACAAAAGGAG TTGTGCTCAC GGAGGCTTCT 
CCTGAAGAAG ATTCATTGCA AGGAGATAAG GGCAGTGGAA AGAAGAAGGC TCAGAGAGGC 
AACTCTATTA CAGAGATAGT GGCAGAAGCG CAAAAGAAAA GTGTAAAACG AGCAACTGAT 
AAAATAAGTG AACGTCGTGG AACACTTCGT CAAATGCAAA TGGGAACATA TGATGAATTG 
GAAGAAGTTG AAGAAGACGA AGATACTGAC GGAGAAATCA GAAGATCATC ATTTGAATAT 
TCAGGAGTTG ATATGATTCA AATGTCACAT GAAAAACAAC TGGAAGATGT GAAAACTTGG 
TGGACGTTGT GTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498054.2
CDS	91..5448
Translation	MTSTTAKLLGLSACRAAALSTTMLTRRKSSIDGTKTERNRRRGESIGGAFTDMINIEPTSIINKSTELLHERFLRDMVRVACLLSMISLCLHTPETIKMWPPLNYIILANDVIVTLIFIGEAAVTINQNGLFDNQNSYLRDRWYQFEFFLLINHILSCVIHIYELCSIWFPALNFVYYPWLGALRSARPFIFLRFIRSIVRFKLPKNRIKLIIKRSSQQIQNVTIFFMFFVFSYAIMGVQLFGRLNYHCVVNGTDPNNVTIADLAIPDTMCSQKGAGGYECPGNMVCMRLQLKPQEEGFYGQFSDFASSLFTVYLAASQEGWVYVLYDCLDSLPSFLAFFYFVTLIFFLAWLVKNVFIAVITETFAEIRVQFSEMWQTREATTDHVYTQKLEKDEDGWKLVEVDKYNRAHSNNSLFLHTIVTSTAFQTVMQLLILANAIFHATFVFYHDESDQIRKIWYYYVEVGFTILFNTEVIIKIYAFGWKAYIARGQHKFDCILCVGSSLNAIWVLYETNIFTYFQVFRIARLIKASPMLEDFVYKIFGPGKKLGGLVIFTGILLIVTSAISLQLFCYVPKLNKFTNFAVAFMSMFQIITQEGWTDVVIEILRACNEQAVPFVAIYFVAYHLLVTLFVLSLFVAVILDNLEMDEELKKVKQLKAREQDTIKTTLPMRLRIFNRFPTAPTMVTMKKVSSEFPLPKIRDSFTRQFADEFVETSDDTIQEIGFKVRSMLSGRGPSKETRITTTIRHVGQLSNKTILTSMLTESNRNRALFSESNQHLASLTRSNTSSKHGKSGALSTNSRSRTRGLASLKGKHMVEGFKENGDLRPEDTARKVEKHGEIDFKALQMKRAHAEITRNRIEEEMRENHPMFDRPLFLVGRESSLRRMCQLIAHSRHSYDQNDGQHRKHSNKYKQFHDFLAIMTYMDWTMVLVTTLSCCSMLWESPWPTTGENLIFNNFYLQIAEYIFVLVMSFELIVKCIANGLFFTPKALVTDVGDILTIFIYITSLMFLIWMPNHIEINSWAQLLMVCRAMRPLRVYALIPHIRRVVVELCRGFREILLVTILLVVLMFIFASFGVQLVGGKLASCNDPMITSRENCTGLYDVKLFVTRMEVYGKNDNLMHPSIIVPRVWTNPRNFNFDHIGNAMLALFETLSYKGWNVVRDVLYLRHGAWAVLFIHIYVFIGCMIGLTLFVGVVVANYTENRGTALLTVDQRRWHDLKARLKMAQPLHVPPKPPESAKLRCYLYDLTTSRWFKQLFAVLVVVNSFTLVIPWNVSEEQDRKTFLLCLTVISAICNILFTLECLLKMIAFTLSGFWQSRRNRIDFIITILGINWIVFHFLFQLPAYFAGGITEWKRLTYTYGYLVVILRFFTIAGRKSTLKMLMLTVVMSMVRSSFIIAAMFLLVLFYANAGVVLFGMVKYGQAVGKHVNFRNGREALVVLFRSVTGEDWNDIMHDCMRAPPFCNWHPGLSYWQTDCGNYVGAIVYFCSFYLIITYIVLNLLVAIIMENFSLFYSSEEDALLSYADIRNFQLVWNMVDIEQKRSIPVRRVKFLLRLLKGRLEVNDESDGLLFKHMCHEMERLHNGDDVSFHDVLNMLSYRSVDIRKSLQLEELLQREELEYIIEEEVAKHTIRAWLENCLKNIKAKQNNTLGKMSSIGSTFAFPQSQEVLTKGVVLTEASPEEDSLQGDKGSGKKKAQRGNSITEIVAEAQKKSVKRATDKISERRGTLRQMQMGTYDELEEVEEDEDTDGEIRRSSFEYSGVDMIQMSHEKQLEDVKTWWTLCD

Target ORF information:

RefSeq Version	NM_065653.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel (nca-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065653.5

ORF Insert Sequence:

ATGACTTCAA CAACTGCAAA ATTGTTGGGA CTGTCAGCGT GCCGTGCGGC CGCATTGTCG 
ACGACAATGC TGACGCGCCG CAAATCGTCA ATAGATGGAA CAAAAACAGA AAGAAATCGG 
AGAAGAGGAG AGTCGATTGG TGGAGCATTT ACTGATATGA TTAATATTGA ACCAACGAGT 
ATTATTAATA AATCGACAGA ATTACTCCAC GAGAGATTTC TCCGAGATAT GGTTCGTGTG 
GCTTGTCTTT TATCAATGAT ATCATTATGT CTTCATACAC CAGAAACTAT CAAAATGTGG 
CCACCACTAA ATTATATAAT TCTGGCGAAT GACGTCATTG TCACTTTGAT TTTTATTGGA 
GAAGCCGCTG TTACGATTAA TCAGAACGGT TTATTCGATA ATCAAAACTC GTATTTACGG 
GATCGATGGT ATCAATTTGA ATTTTTTCTT CTCATTAATC ACATTCTATC ATGTGTTATT 
CATATTTATG AACTTTGCTC AATATGGTTT CCAGCACTTA ATTTTGTGTA TTATCCATGG 
CTTGGAGCAC TTCGATCAGC TCGACCATTC ATTTTTCTTC GATTTATTCG ATCAATAGTG 
AGGTTTAAAC TTCCCAAAAA TCGAATTAAA TTAATCATAA AACGATCATC TCAACAAATC 
CAAAATGTTA CAATATTCTT CATGTTTTTT GTATTTTCGT ATGCAATAAT GGGTGTTCAA 
TTATTTGGAC GACTCAATTA TCACTGCGTT GTCAATGGAA CTGATCCAAA TAATGTTACA 
ATTGCCGATC TAGCAATCCC TGACACAATG TGCTCACAGA AAGGAGCTGG TGGTTACGAG 
TGCCCAGGGA ATATGGTTTG TATGAGATTA CAACTGAAAC CACAAGAAGA AGGATTCTAC 
GGACAATTTA GTGATTTTGC AAGCTCTTTG TTCACAGTTT ATCTGGCTGC ATCTCAAGAA 
GGATGGGTTT ATGTTTTATA TGATTGTTTG GATTCATTAC CTTCATTTCT TGCATTTTTC 
TATTTTGTAA CGCTCATTTT CTTTCTGGCG TGGTTGGTGA AGAATGTTTT CATCGCCGTC 
ATTACCGAAA CATTTGCTGA GATTCGTGTT CAATTCAGTG AAATGTGGCA GACACGTGAA 
GCAACAACTG ATCATGTGTA CACACAGAAA CTGGAAAAAG ATGAAGATGG ATGGAAATTG 
GTGGAAGTTG ATAAATACAA TAGAGCTCAT TCTAATAATT CACTTTTCTT GCATACAATA 
GTCACTTCAA CAGCTTTTCA AACAGTCATG CAATTGTTAA TCCTTGCAAA TGCAATATTT 
CATGCTACTT TTGTATTTTA TCATGATGAA TCCGATCAAA TCAGGAAAAT TTGGTATTAT 
TACGTGGAAG TTGGCTTTAC GATACTCTTT AATACGGAAG TCATCATAAA GATTTATGCA 
TTTGGATGGA AAGCTTATAT TGCACGTGGG CAACATAAAT TTGATTGTAT TCTTTGCGTT 
GGTTCAAGCC TCAATGCAAT ATGGGTTCTT TATGAAACAA ATATCTTCAC ATACTTCCAA 
GTTTTCCGAA TTGCTCGACT TATCAAAGCC TCTCCAATGC TAGAAGACTT CGTTTATAAA 
ATCTTTGGAC CCGGAAAGAA ATTGGGAGGT CTTGTGATAT TCACTGGAAT TCTTTTGATC 
GTTACTTCTG CAATTTCGTT GCAGCTATTT TGCTATGTGC CAAAATTAAA CAAATTTACC 
AATTTTGCTG TGGCTTTCAT GTCAATGTTC CAAATAATAA CACAAGAAGG CTGGACAGAT 
GTTGTCATTG AAATTCTAAG AGCATGTAAT GAACAAGCGG TTCCATTTGT TGCCATCTAT 
TTTGTTGCTT ATCACTTACT GGTTACACTG TTCGTTTTGT CACTCTTCGT TGCCGTCATT 
CTTGATAATT TGGAAATGGA CGAAGAATTG AAAAAAGTGA AACAACTGAA AGCAAGAGAA 
CAGGATACAA TAAAGACAAC ACTTCCGATG CGTCTCCGCA TTTTCAATCG ATTTCCGACT 
GCTCCAACAA TGGTTACAAT GAAGAAAGTA TCGTCAGAGT TTCCTCTTCC AAAAATTAGA 
GATTCGTTTA CAAGACAGTT TGCGGATGAA TTCGTAGAGA CAAGTGATGA TACTATTCAG 
GAAATCGGAT TTAAAGTCCG ATCAATGTTA TCCGGTAGAG GACCATCTAA AGAAACACGT 
ATAACAACAA CAATCCGACA TGTTGGACAA CTTTCAAACA AAACAATTCT CACCTCAATG 
CTCACTGAAT CAAATCGGAA TCGTGCTCTT TTCTCGGAAT CAAATCAACA TTTGGCTAGT 
TTAACCAGAA GTAACACTTC ATCAAAACAC GGAAAAAGTG GAGCTTTATC AACGAATTCT 
CGATCAAGAA CACGTGGATT GGCTTCATTA AAAGGGAAAC ATATGGTGGA AGGATTCAAA 
GAAAATGGAG ATTTACGACC AGAAGACACT GCTAGAAAAG TGGAGAAGCA TGGGGAGATT 
GATTTTAAAG CATTGCAAAT GAAAAGGGCT CATGCTGAAA TTACAAGAAA CCGAATAGAA 
GAAGAAATGC GCGAGAACCA TCCGATGTTC GATCGTCCGC TCTTTCTTGT TGGCAGAGAA 
TCGTCTCTAC GTAGAATGTG CCAACTCATT GCTCATTCCA GACATTCATA CGATCAAAAT 
GATGGCCAAC ACAGAAAACA TTCGAATAAA TATAAACAAT TTCATGACTT TCTGGCCATC 
ATGACATATA TGGATTGGAC AATGGTGCTA GTAACAACCT TATCATGTTG CAGTATGTTA 
TGGGAATCAC CTTGGCCAAC GACAGGAGAG AATCTGATTT TTAATAACTT TTATCTCCAA 
ATTGCTGAAT ACATCTTTGT GCTTGTGATG AGTTTCGAAC TAATTGTAAA ATGCATTGCA 
AATGGTCTCT TCTTCACACC AAAAGCCTTG GTAACAGATG TCGGAGATAT TCTTACGATT 
TTCATTTATA TTACAAGTCT AATGTTTCTG ATATGGATGC CAAATCACAT CGAAATCAAC 
AGCTGGGCTC AGCTTCTTAT GGTTTGCCGA GCAATGAGAC CATTGAGAGT TTACGCGTTG 
ATTCCGCATA TTCGAAGAGT CGTTGTGGAG TTATGCAGAG GATTTCGGGA AATTTTATTG 
GTAACAATTC TTCTCGTCGT TCTCATGTTC ATTTTCGCAA GTTTCGGAGT TCAACTAGTC 
GGTGGAAAGC TAGCATCATG CAATGATCCA ATGATAACAT CTCGTGAAAA CTGTACAGGA 
TTGTATGACG TCAAATTGTT TGTTACTCGG ATGGAAGTTT ATGGTAAAAA TGACAATCTG 
ATGCATCCTT CAATCATTGT GCCACGTGTA TGGACAAATC CACGGAATTT CAATTTCGAT 
CACATTGGGA ATGCAATGTT GGCTCTTTTT GAAACACTTT CCTACAAGGG ATGGAATGTA 
GTCAGAGATG TTTTATACTT GCGACATGGA GCTTGGGCAG TTCTCTTCAT TCACATTTAT 
GTATTCATTG GATGTATGAT TGGACTAACA CTATTCGTCG GTGTGGTTGT TGCAAATTAC 
ACTGAAAACC GTGGAACAGC CCTTCTAACA GTTGATCAAC GTCGTTGGCA TGATCTGAAG 
GCTCGTCTGA AAATGGCTCA ACCTCTACAT GTTCCACCGA AACCTCCGGA ATCTGCAAAA 
CTGCGTTGTT ACCTCTATGA TCTGACGACG TCTCGATGGT TCAAACAGTT ATTTGCTGTT 
CTTGTGGTTG TCAATTCATT TACACTTGTT ATTCCATGGA ATGTTAGTGA AGAACAGGAT 
AGAAAGACTT TTCTGTTATG TCTTACTGTC ATTTCTGCAA TTTGCAATAT TTTATTCACT 
TTGGAATGCT TGCTGAAAAT GATTGCGTTC ACGTTATCGG GATTTTGGCA ATCAAGAAGA 
AATCGAATTG ATTTTATTAT CACTATCCTC GGAATCAATT GGATTGTTTT TCATTTTTTG 
TTTCAATTAC CTGCGTACTT TGCTGGTGGA ATAACCGAAT GGAAGCGTCT CACATACACT 
TATGGATATC TCGTCGTTAT ACTCCGTTTT TTCACAATTG CCGGCCGTAA ATCCACACTG 
AAAATGTTAA TGTTAACAGT TGTAATGTCA ATGGTTCGAT CATCATTTAT TATTGCTGCA 
ATGTTTCTAT TGGTTCTTTT TTATGCAAAT GCAGGAGTTG TGTTATTTGG AATGGTCAAA 
TATGGACAAG CTGTTGGAAA ACATGTAAAT TTCCGAAATG GACGAGAGGC ATTGGTGGTG 
TTATTTCGAT CAGTTACCGG TGAAGATTGG AATGATATTA TGCACGATTG TATGAGAGCA 
CCACCATTTT GCAATTGGCA TCCAGGGCTC AGCTATTGGC AAACTGATTG TGGGAATTAT 
GTTGGAGCAA TTGTTTATTT TTGCTCCTTC TATCTCATTA TCACATATAT TGTGCTCAAT 
CTTCTTGTCG CTATCATCAT GGAGAACTTC TCATTGTTCT ACTCATCTGA AGAAGATGCA 
CTTCTCTCGT ATGCCGATAT TCGAAATTTC CAACTTGTTT GGAATATGGT GGATATTGAA 
CAGAAACGAT CAATTCCAGT GAGACGTGTC AAATTTCTGT TGAGATTGTT AAAAGGAAGA 
CTGGAAGTTA ATGATGAATC CGATGGATTG TTGTTTAAAC ATATGTGTCA TGAAATGGAA 
CGATTGCATA ATGGAGATGA TGTTTCATTT CATGATGTTC TCAACATGCT CTCGTATCGA 
AGTGTGGATA TTCGAAAAAG TTTACAACTG GAAGAATTAC TTCAAAGAGA AGAACTTGAA 
TACATAATTG AAGAAGAAGT TGCAAAGCAC ACTATTCGGG CGTGGCTAGA GAATTGTTTG 
AAAAACATCA AAGCCAAGCA GAATAATACT CTTGGAAAAA TGAGCTCAAT TGGGTCAACA 
TTTGCATTTC CACAATCTCA AGAAGTATTA ACAAAAGGAG TTGTGCTCAC GGAGGCTTCT 
CCTGAAGAAG ATTCATTGCA AGGAGATAAG GGCAGTGGAA AGAAGAAGGC TCAGAGAGGC 
AACTCTATTA CAGAGATAGT GGCAGAAGCG CAAAAGAAAA GTGTAAAACG AGCAACTGAT 
AAAATAAGTG AACGTCGTGG AACACTTCGT CAAATGCAAA TGGGAACATA TGATGAATTG 
GAAGAAGTTG AAGAAGACGA AGATACTGAC GGAGAAATCA GAAGATCATC ATTTGAATAT 
TCAGGAGTTG ATATGATTCA AATGTCACAT GAAAAACAAC TGGAAGATGT GAAAACTTGG 
TGGACGTTGT GTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nca-2 ( NM_001136363.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001129835.1 Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel (nca-2), partial mRNA.

Price & Availability↑

CloneID	OCa341131
Clone ID Related Accession (Same CDS sequence)	NM_001136363.2 , NM_001136363.3
Accession Version	NM_001136363.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554652800000
Organism	Caenorhabditis elegans
Product	Novel Channel type/putative Nematode CAlcium channel
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_001136363.1. COMPLETENESS: incomplete on both ends.

ATGCTGACGC GCCGCAAATC GTCAATAGAT GGAACAAAAA CAGAAAGAAA TCGGAGAAGA 
GGAGAGTCGA TTGGTGGAGC ATTTACTGAT ATGATTAATA TTGAACCAAC GAGTATTATT 
AATAAATCGA CAGAATTACT CCACGAGAGA TTTCTCCGAG ATATGGTTCG TGTGGCTTGT 
CTTTTATCAA TGATATCATT ATGTCTTCAT ACACCAGAAA CTATCAAAAT GTGGCCACCA 
CTAAATTATA TAATTCTGGC GAATGACGTC ATTGTCACTT TGATTTTTAT TGGAGAAGCC 
GCTGTTACGA TTAATCAGAA CGGTTTATTC GATAATCAAA ACTCGTATTT ACGGGATCGA 
TGGTATCAAT TTGAATTTTT TCTTCTCATT AATCACATTC TATCATGTGT TATTCATATT 
TATGAACTTT GCTCAATATG GTTTCCAGCA CTTAATTTTG TGTATTATCC ATGGCTTGGA 
GCACTTCGAT CAGCTCGACC ATTCATTTTT CTTCGATTTA TTCGATCAAT AGTGAGGTTT 
AAACTTCCCA AAAATCGAAT TAAATTAATC ATAAAACGAT CATCTCAACA AATCCAAAAT 
GTTACAATAT TCTTCATGTT TTTTGTATTT TCGTATGCAA TAATGGGTGT TCAATTATTT 
GGACGACTCA ATTATCACTG CGTTGTCAAT GGAACTGATC CAAATAATGT TACAATTGCC 
GATCTAGCAA TCCCTGACAC AATGTGCTCA CAGAAAGGAG CTGGTGGTTA CGAGTGCCCA 
GGGAATATGG TTTGTATGAG ATTACAACTG AAACCACAAG AAGAAGGATT CTACGGACAA 
TTTAGTGATT TTGCAAGCTC TTTGTTCACA GTTTATCTGG CTGCATCTCA AGAAGGATGG 
GTTTATGTTT TATATGATTG TTTGGATTCA TTACCTTCAT TTCTTGCATT TTTCTATTTT 
GTAACGCTCA TTTTCTTTCT GGCGTGGTTG GTGAAGAATG TTTTCATCGC CGTCATTACC 
GAAACATTTG CTGAGATTCG TGTTCAATTC AGTGAAATGT GGCAGACACG TGAAGCAACA 
ACTGATCATG TGTACACACA GAAACTGGAA AAAGATGAAG ATGGATGGAA ATTGGTGGAA 
GTTGATAAAT ACAATAGAGC TCATTCTAAT AATTCACTTT TCTTGCATAC AATAGTCACT 
TCAACAGCTT TTCAAACAGT CATGCAATTG TTAATCCTTG CAAATGCAAT ATTTCATGCT 
ACTTTTGTAT TTTATCATGA TGAATCCGAT CAAATCAGGA AAATTTGGTA TTATTACGTG 
GAAGTTGGCT TTACGATACT CTTTAATACG GAAGTCATCA TAAAGATTTA TGCATTTGGA 
TGGAAAGCTT ATATTGCACG TGGGCAACAT AAATTTGATT GTATTCTTTG CGTTGGTTCA 
AGCCTCAATG CAATATGGGT TCTTTATGAA ACAAATATCT TCACATACTT CCAAGTTTTC 
CGAATTGCTC GACTTATCAA AGCCTCTCCA ATGCTAGAAG ACTTCGTTTA TAAAATCTTT 
GGACCCGGAA AGAAATTGGG AGGTCTTGTG ATATTCACTG GAATTCTTTT GATCGTTACT 
TCTGCAATTT CGTTGCAGCT ATTTTGCTAT GTGCCAAAAT TAAACAAATT TACCAATTTT 
GCTGTGGCTT TCATGTCAAT GTTCCAAATA ATAACACAAG AAGGCTGGAC AGATGTTGTC 
ATTGAAATTC TAAGAGCATG TAATGAACAA GCGGTTCCAT TTGTTGCCAT CTATTTTGTT 
GCTTATCACT TACTGGTTAC ACTGTTCGTT TTGTCACTCT TCGTTGCCGT CATTCTTGAT 
AATTTGGAAA TGGACGAAGA ATTGAAAAAA GTGAAACAAC TGAAAGCAAG AGAACAGGAT 
ACAATAAAGA CAACACTTCC GATGCGTCTC CGCATTTTCA ATCGATTTCC GACTGCTCCA 
ACAATGGTTA CAATGAAGAA AGTATCGTCA GAGTTTCCTC TTCCAAAAAT TAGAGATTCG 
TTTACAAGAC AGTTTGCGGA TGAATTCGTA GAGACAAGTG ATGATACTAT TCAGGAAATC 
GGATTTAAAG TCCGATCAAT GTTATCCGGT AGAGGACCAT CTAAAGAAAC ACGTATAACA 
ACAACAATCC GACATGTTGG ACAACTTTCA AACAAAACAA TTCTCACCTC AATGCTCACT 
GAATCAAATC GGAATCGTGC TCTTTTCTCG GAATCAAATC AACATTTGGC TAGTTTAACC 
AGAAGTAACA CTTCATCAAA ACACGGAAAA AGTGGAGCTT TATCAACGAA TTCTCGATCA 
AGAACACGTG GATTGGCTTC ATTAAAAGGG AAACATATGG TGGAAGGATT CAAAGAAAAT 
GGAGATTTAC GACCAGAAGA CACTGCTAGA AAAGTGGAGA AGCATGGGGA GATTGATTTT 
AAAGCATTGC AAATGAAAAG GGCTCATGCT GAAATTACAA GAAACCGAAT AGAAGAAGAA 
ATGCGCGAGA ACCATCCGAT GTTCGATCGT CCGCTCTTTC TTGTTGGCAG AGAATCGTCT 
CTACGTAGAA TGTGCCAACT CATTGCTCAT TCCAGACATT CATACGATCA AAATGATGGC 
CAACACAGAA AACATTCGAA TAAATATAAA CAATTTCATG ACTTTCTGGC CATCATGACA 
TATATGGATT GGACAATGGT GCTAGTAACA ACCTTATCAT GTTGCAGTAT GTTATGGGAA 
TCACCTTGGC CAACGACAGG AGAGAATCTG ATTTTTAATA ACTTTTATCT CCAAATTGCT 
GAATACATCT TTGTGCTTGT GATGAGTTTC GAACTAATTG TAAAATGCAT TGCAAATGGT 
CTCTTCTTCA CACCAAAAGC CTTGGTAACA GATGTCGGAG ATATTCTTAC GATTTTCATT 
TATATTACAA GTCTAATGTT TCTGATATGG ATGCCAAATC ACATCGAAAT CAACAGCTGG 
GCTCAGCTTC TTATGGTTTG CCGAGCAATG AGACCATTGA GAGTTTACGC GTTGATTCCG 
CATATTCGAA GAGTCGTTGT GGAGTTATGC AGAGGATTTC GGGAAATTTT ATTGGTAACA 
ATTCTTCTCG TCGTTCTCAT GTTCATTTTC GCAAGTTTCG GAGTTCAACT AGTCGGTGGA 
AAGCTAGCAT CATGCAATGA TCCAATGATA ACATCTCGTG AAAACTGTAC AGGATTGTAT 
GACGTCAAAT TGTTTGTTAC TCGGATGGAA GTTTATGGTA AAAATGACAA TCTGATGCAT 
CCTTCAATCA TTGTGCCACG TGTATGGACA AATCCACGGA ATTTCAATTT CGATCACATT 
GGGAATGCAA TGTTGGCTCT TTTTGAAACA CTTTCCTACA AGGGATGGAA TGTAGTCAGA 
GATGTTTTAT ACTTGCGACA TGGAGCTTGG GCAGTTCTCT TCATTCACAT TTATGTATTC 
ATTGGATGTA TGATTGGACT AACACTATTC GTCGGTGTGG TTGTTGCAAA TTACACTGAA 
AACCGTGGAA CAGCCCTTCT AACAGTTGAT CAACGTCGTT GGCATGATCT GAAGGCTCGT 
CTGAAAATGG CTCAACCTCT ACATGTTCCA CCGAAACCTC CGGAATCTGC AAAACTGCGT 
TGTTACCTCT ATGATCTGAC GACGTCTCGA TGGTTCAAAC AGTTATTTGC TGTTCTTGTG 
GTTGTCAATT CATTTACACT TGTTATTCCA TGGAATGTTA GTGAAGAACA GGATAGAAAG 
ACTTTTCTGT TATGTCTTAC TGTCATTTCT GCAATTTGCA ATATTTTATT CACTTTGGAA 
TGCTTGCTGA AAATGATTGC GTTCACGTTA TCGGGATTTT GGCAATCAAG AAGAAATCGA 
ATTGATTTTA TTATCACTAT CCTCGGAATC AATTGGATTG TTTTTCATTT TTTGTTTCAA 
TTACCTGCGT ACTTTGCTGG TGGAATAACC GAATGGAAGC GTCTCACATA CACTTATGGA 
TATCTCGTCG TTATACTCCG TTTTTTCACA ATTGCCGGCC GTAAATCCAC ACTGAAAATG 
TTAATGTTAA CAGTTGTAAT GTCAATGGTT CGATCATCAT TTATTATTGC TGCAATGTTT 
CTATTGGTTC TTTTTTATGC AAATGCAGGA GTTGTGTTAT TTGGAATGGT CAAATATGGA 
CAAGCTGTTG GAAAACATGT AAATTTCCGA AATGGACGAG AGGCATTGGT GGTGTTATTT 
CGATCAGTTA CCGGTGAAGA TTGGAATGAT ATTATGCACG ATTGTATGAG AGCACCACCA 
TTTTGCAATT GGCATCCAGG GCTCAGCTAT TGGCAAACTG ATTGTGGGAA TTATGTTGGA 
GCAATTGTTT ATTTTTGCTC CTTCTATCTC ATTATCACAT ATATTGTGCT CAATCTTCTT 
GTCGCTATCA TCATGGAGAA CTTCTCATTG TTCTACTCAT CTGAAGAAGA TGCACTTCTC 
TCGTATGCCG ATATTCGAAA TTTCCAACTT GTTTGGAATA TGGTGGATAT TGAACAGAAA 
CGATCAATTC CAGTGAGACG TGTCAAATTT CTGTTGAGAT TGTTAAAAGG AAGACTGGAA 
GTTAATGATG AATCCGATGG ATTGTTGTTT AAACATATGT GTCATGAAAT GGAACGATTG 
CATAATGGAG ATGATGTTTC ATTTCATGAT GTTCTCAACA TGCTCTCGTA TCGAAGTGTG 
GATATTCGAA AAAGTTTACA ACTGGAAGAA TTACTTCAAA GAGAAGAACT TGAATACATA 
ATTGAAGAAG AAGTTGCAAA GCACACTATT CGGGCGTGGC TAGAGAATTG TTTGAAAAAC 
ATCAAAGCCA AGCAGAATAA TACTCTTGGA AAAATGAGCT CAATTGGGTC AACATTTGCA 
TTTCCACAAT CTCAAGAAGT ATTAACAAAA GGAGTTGTGC TCACGGAGGC TTCTCCTGAA 
GAAGATTCAT TGCAAGGAGA TAAGGGCAGT GGAAAGAAGA AGGCTCAGAG AGGCAACTCT 
ATTACAGAGA TAGTGGCAGA AGCGCAAAAG AAAAGTGTAA AACGAGCAAC TGATAAAATA 
AGTGAACGTC GTGGAACACT TCGTCAAATG CAAATGGGAA CATATGATGA ATTGGAAGAA 
GTTGAAGAAG ACGAAGATAC TGACGGAGAA ATCAGAAGAT CATCATTTGA ATATTCAGGA 
GTTGATATGA TTCAAATGTC ACATGAAAAA CAACTGGAAG ATGTGAAAAC TTGGTGGACG 
TTGTGTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129835.1
CDS	1..5292
Translation	MLTRRKSSIDGTKTERNRRRGESIGGAFTDMINIEPTSIINKSTELLHERFLRDMVRVACLLSMISLCLHTPETIKMWPPLNYIILANDVIVTLIFIGEAAVTINQNGLFDNQNSYLRDRWYQFEFFLLINHILSCVIHIYELCSIWFPALNFVYYPWLGALRSARPFIFLRFIRSIVRFKLPKNRIKLIIKRSSQQIQNVTIFFMFFVFSYAIMGVQLFGRLNYHCVVNGTDPNNVTIADLAIPDTMCSQKGAGGYECPGNMVCMRLQLKPQEEGFYGQFSDFASSLFTVYLAASQEGWVYVLYDCLDSLPSFLAFFYFVTLIFFLAWLVKNVFIAVITETFAEIRVQFSEMWQTREATTDHVYTQKLEKDEDGWKLVEVDKYNRAHSNNSLFLHTIVTSTAFQTVMQLLILANAIFHATFVFYHDESDQIRKIWYYYVEVGFTILFNTEVIIKIYAFGWKAYIARGQHKFDCILCVGSSLNAIWVLYETNIFTYFQVFRIARLIKASPMLEDFVYKIFGPGKKLGGLVIFTGILLIVTSAISLQLFCYVPKLNKFTNFAVAFMSMFQIITQEGWTDVVIEILRACNEQAVPFVAIYFVAYHLLVTLFVLSLFVAVILDNLEMDEELKKVKQLKAREQDTIKTTLPMRLRIFNRFPTAPTMVTMKKVSSEFPLPKIRDSFTRQFADEFVETSDDTIQEIGFKVRSMLSGRGPSKETRITTTIRHVGQLSNKTILTSMLTESNRNRALFSESNQHLASLTRSNTSSKHGKSGALSTNSRSRTRGLASLKGKHMVEGFKENGDLRPEDTARKVEKHGEIDFKALQMKRAHAEITRNRIEEEMRENHPMFDRPLFLVGRESSLRRMCQLIAHSRHSYDQNDGQHRKHSNKYKQFHDFLAIMTYMDWTMVLVTTLSCCSMLWESPWPTTGENLIFNNFYLQIAEYIFVLVMSFELIVKCIANGLFFTPKALVTDVGDILTIFIYITSLMFLIWMPNHIEINSWAQLLMVCRAMRPLRVYALIPHIRRVVVELCRGFREILLVTILLVVLMFIFASFGVQLVGGKLASCNDPMITSRENCTGLYDVKLFVTRMEVYGKNDNLMHPSIIVPRVWTNPRNFNFDHIGNAMLALFETLSYKGWNVVRDVLYLRHGAWAVLFIHIYVFIGCMIGLTLFVGVVVANYTENRGTALLTVDQRRWHDLKARLKMAQPLHVPPKPPESAKLRCYLYDLTTSRWFKQLFAVLVVVNSFTLVIPWNVSEEQDRKTFLLCLTVISAICNILFTLECLLKMIAFTLSGFWQSRRNRIDFIITILGINWIVFHFLFQLPAYFAGGITEWKRLTYTYGYLVVILRFFTIAGRKSTLKMLMLTVVMSMVRSSFIIAAMFLLVLFYANAGVVLFGMVKYGQAVGKHVNFRNGREALVVLFRSVTGEDWNDIMHDCMRAPPFCNWHPGLSYWQTDCGNYVGAIVYFCSFYLIITYIVLNLLVAIIMENFSLFYSSEEDALLSYADIRNFQLVWNMVDIEQKRSIPVRRVKFLLRLLKGRLEVNDESDGLLFKHMCHEMERLHNGDDVSFHDVLNMLSYRSVDIRKSLQLEELLQREELEYIIEEEVAKHTIRAWLENCLKNIKAKQNNTLGKMSSIGSTFAFPQSQEVLTKGVVLTEASPEEDSLQGDKGSGKKKAQRGNSITEIVAEAQKKSVKRATDKISERRGTLRQMQMGTYDELEEVEEDEDTDGEIRRSSFEYSGVDMIQMSHEKQLEDVKTWWTLCD

Target ORF information:

RefSeq Version	NM_001136363.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel (nca-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136363.2

ORF Insert Sequence:

ATGCTGACGC GCCGCAAATC GTCAATAGAT GGAACAAAAA CAGAAAGAAA TCGGAGAAGA 
GGAGAGTCGA TTGGTGGAGC ATTTACTGAT ATGATTAATA TTGAACCAAC GAGTATTATT 
AATAAATCGA CAGAATTACT CCACGAGAGA TTTCTCCGAG ATATGGTTCG TGTGGCTTGT 
CTTTTATCAA TGATATCATT ATGTCTTCAT ACACCAGAAA CTATCAAAAT GTGGCCACCA 
CTAAATTATA TAATTCTGGC GAATGACGTC ATTGTCACTT TGATTTTTAT TGGAGAAGCC 
GCTGTTACGA TTAATCAGAA CGGTTTATTC GATAATCAAA ACTCGTATTT ACGGGATCGA 
TGGTATCAAT TTGAATTTTT TCTTCTCATT AATCACATTC TATCATGTGT TATTCATATT 
TATGAACTTT GCTCAATATG GTTTCCAGCA CTTAATTTTG TGTATTATCC ATGGCTTGGA 
GCACTTCGAT CAGCTCGACC ATTCATTTTT CTTCGATTTA TTCGATCAAT AGTGAGGTTT 
AAACTTCCCA AAAATCGAAT TAAATTAATC ATAAAACGAT CATCTCAACA AATCCAAAAT 
GTTACAATAT TCTTCATGTT TTTTGTATTT TCGTATGCAA TAATGGGTGT TCAATTATTT 
GGACGACTCA ATTATCACTG CGTTGTCAAT GGAACTGATC CAAATAATGT TACAATTGCC 
GATCTAGCAA TCCCTGACAC AATGTGCTCA CAGAAAGGAG CTGGTGGTTA CGAGTGCCCA 
GGGAATATGG TTTGTATGAG ATTACAACTG AAACCACAAG AAGAAGGATT CTACGGACAA 
TTTAGTGATT TTGCAAGCTC TTTGTTCACA GTTTATCTGG CTGCATCTCA AGAAGGATGG 
GTTTATGTTT TATATGATTG TTTGGATTCA TTACCTTCAT TTCTTGCATT TTTCTATTTT 
GTAACGCTCA TTTTCTTTCT GGCGTGGTTG GTGAAGAATG TTTTCATCGC CGTCATTACC 
GAAACATTTG CTGAGATTCG TGTTCAATTC AGTGAAATGT GGCAGACACG TGAAGCAACA 
ACTGATCATG TGTACACACA GAAACTGGAA AAAGATGAAG ATGGATGGAA ATTGGTGGAA 
GTTGATAAAT ACAATAGAGC TCATTCTAAT AATTCACTTT TCTTGCATAC AATAGTCACT 
TCAACAGCTT TTCAAACAGT CATGCAATTG TTAATCCTTG CAAATGCAAT ATTTCATGCT 
ACTTTTGTAT TTTATCATGA TGAATCCGAT CAAATCAGGA AAATTTGGTA TTATTACGTG 
GAAGTTGGCT TTACGATACT CTTTAATACG GAAGTCATCA TAAAGATTTA TGCATTTGGA 
TGGAAAGCTT ATATTGCACG TGGGCAACAT AAATTTGATT GTATTCTTTG CGTTGGTTCA 
AGCCTCAATG CAATATGGGT TCTTTATGAA ACAAATATCT TCACATACTT CCAAGTTTTC 
CGAATTGCTC GACTTATCAA AGCCTCTCCA ATGCTAGAAG ACTTCGTTTA TAAAATCTTT 
GGACCCGGAA AGAAATTGGG AGGTCTTGTG ATATTCACTG GAATTCTTTT GATCGTTACT 
TCTGCAATTT CGTTGCAGCT ATTTTGCTAT GTGCCAAAAT TAAACAAATT TACCAATTTT 
GCTGTGGCTT TCATGTCAAT GTTCCAAATA ATAACACAAG AAGGCTGGAC AGATGTTGTC 
ATTGAAATTC TAAGAGCATG TAATGAACAA GCGGTTCCAT TTGTTGCCAT CTATTTTGTT 
GCTTATCACT TACTGGTTAC ACTGTTCGTT TTGTCACTCT TCGTTGCCGT CATTCTTGAT 
AATTTGGAAA TGGACGAAGA ATTGAAAAAA GTGAAACAAC TGAAAGCAAG AGAACAGGAT 
ACAATAAAGA CAACACTTCC GATGCGTCTC CGCATTTTCA ATCGATTTCC GACTGCTCCA 
ACAATGGTTA CAATGAAGAA AGTATCGTCA GAGTTTCCTC TTCCAAAAAT TAGAGATTCG 
TTTACAAGAC AGTTTGCGGA TGAATTCGTA GAGACAAGTG ATGATACTAT TCAGGAAATC 
GGATTTAAAG TCCGATCAAT GTTATCCGGT AGAGGACCAT CTAAAGAAAC ACGTATAACA 
ACAACAATCC GACATGTTGG ACAACTTTCA AACAAAACAA TTCTCACCTC AATGCTCACT 
GAATCAAATC GGAATCGTGC TCTTTTCTCG GAATCAAATC AACATTTGGC TAGTTTAACC 
AGAAGTAACA CTTCATCAAA ACACGGAAAA AGTGGAGCTT TATCAACGAA TTCTCGATCA 
AGAACACGTG GATTGGCTTC ATTAAAAGGG AAACATATGG TGGAAGGATT CAAAGAAAAT 
GGAGATTTAC GACCAGAAGA CACTGCTAGA AAAGTGGAGA AGCATGGGGA GATTGATTTT 
AAAGCATTGC AAATGAAAAG GGCTCATGCT GAAATTACAA GAAACCGAAT AGAAGAAGAA 
ATGCGCGAGA ACCATCCGAT GTTCGATCGT CCGCTCTTTC TTGTTGGCAG AGAATCGTCT 
CTACGTAGAA TGTGCCAACT CATTGCTCAT TCCAGACATT CATACGATCA AAATGATGGC 
CAACACAGAA AACATTCGAA TAAATATAAA CAATTTCATG ACTTTCTGGC CATCATGACA 
TATATGGATT GGACAATGGT GCTAGTAACA ACCTTATCAT GTTGCAGTAT GTTATGGGAA 
TCACCTTGGC CAACGACAGG AGAGAATCTG ATTTTTAATA ACTTTTATCT CCAAATTGCT 
GAATACATCT TTGTGCTTGT GATGAGTTTC GAACTAATTG TAAAATGCAT TGCAAATGGT 
CTCTTCTTCA CACCAAAAGC CTTGGTAACA GATGTCGGAG ATATTCTTAC GATTTTCATT 
TATATTACAA GTCTAATGTT TCTGATATGG ATGCCAAATC ACATCGAAAT CAACAGCTGG 
GCTCAGCTTC TTATGGTTTG CCGAGCAATG AGACCATTGA GAGTTTACGC GTTGATTCCG 
CATATTCGAA GAGTCGTTGT GGAGTTATGC AGAGGATTTC GGGAAATTTT ATTGGTAACA 
ATTCTTCTCG TCGTTCTCAT GTTCATTTTC GCAAGTTTCG GAGTTCAACT AGTCGGTGGA 
AAGCTAGCAT CATGCAATGA TCCAATGATA ACATCTCGTG AAAACTGTAC AGGATTGTAT 
GACGTCAAAT TGTTTGTTAC TCGGATGGAA GTTTATGGTA AAAATGACAA TCTGATGCAT 
CCTTCAATCA TTGTGCCACG TGTATGGACA AATCCACGGA ATTTCAATTT CGATCACATT 
GGGAATGCAA TGTTGGCTCT TTTTGAAACA CTTTCCTACA AGGGATGGAA TGTAGTCAGA 
GATGTTTTAT ACTTGCGACA TGGAGCTTGG GCAGTTCTCT TCATTCACAT TTATGTATTC 
ATTGGATGTA TGATTGGACT AACACTATTC GTCGGTGTGG TTGTTGCAAA TTACACTGAA 
AACCGTGGAA CAGCCCTTCT AACAGTTGAT CAACGTCGTT GGCATGATCT GAAGGCTCGT 
CTGAAAATGG CTCAACCTCT ACATGTTCCA CCGAAACCTC CGGAATCTGC AAAACTGCGT 
TGTTACCTCT ATGATCTGAC GACGTCTCGA TGGTTCAAAC AGTTATTTGC TGTTCTTGTG 
GTTGTCAATT CATTTACACT TGTTATTCCA TGGAATGTTA GTGAAGAACA GGATAGAAAG 
ACTTTTCTGT TATGTCTTAC TGTCATTTCT GCAATTTGCA ATATTTTATT CACTTTGGAA 
TGCTTGCTGA AAATGATTGC GTTCACGTTA TCGGGATTTT GGCAATCAAG AAGAAATCGA 
ATTGATTTTA TTATCACTAT CCTCGGAATC AATTGGATTG TTTTTCATTT TTTGTTTCAA 
TTACCTGCGT ACTTTGCTGG TGGAATAACC GAATGGAAGC GTCTCACATA CACTTATGGA 
TATCTCGTCG TTATACTCCG TTTTTTCACA ATTGCCGGCC GTAAATCCAC ACTGAAAATG 
TTAATGTTAA CAGTTGTAAT GTCAATGGTT CGATCATCAT TTATTATTGC TGCAATGTTT 
CTATTGGTTC TTTTTTATGC AAATGCAGGA GTTGTGTTAT TTGGAATGGT CAAATATGGA 
CAAGCTGTTG GAAAACATGT AAATTTCCGA AATGGACGAG AGGCATTGGT GGTGTTATTT 
CGATCAGTTA CCGGTGAAGA TTGGAATGAT ATTATGCACG ATTGTATGAG AGCACCACCA 
TTTTGCAATT GGCATCCAGG GCTCAGCTAT TGGCAAACTG ATTGTGGGAA TTATGTTGGA 
GCAATTGTTT ATTTTTGCTC CTTCTATCTC ATTATCACAT ATATTGTGCT CAATCTTCTT 
GTCGCTATCA TCATGGAGAA CTTCTCATTG TTCTACTCAT CTGAAGAAGA TGCACTTCTC 
TCGTATGCCG ATATTCGAAA TTTCCAACTT GTTTGGAATA TGGTGGATAT TGAACAGAAA 
CGATCAATTC CAGTGAGACG TGTCAAATTT CTGTTGAGAT TGTTAAAAGG AAGACTGGAA 
GTTAATGATG AATCCGATGG ATTGTTGTTT AAACATATGT GTCATGAAAT GGAACGATTG 
CATAATGGAG ATGATGTTTC ATTTCATGAT GTTCTCAACA TGCTCTCGTA TCGAAGTGTG 
GATATTCGAA AAAGTTTACA ACTGGAAGAA TTACTTCAAA GAGAAGAACT TGAATACATA 
ATTGAAGAAG AAGTTGCAAA GCACACTATT CGGGCGTGGC TAGAGAATTG TTTGAAAAAC 
ATCAAAGCCA AGCAGAATAA TACTCTTGGA AAAATGAGCT CAATTGGGTC AACATTTGCA 
TTTCCACAAT CTCAAGAAGT ATTAACAAAA GGAGTTGTGC TCACGGAGGC TTCTCCTGAA 
GAAGATTCAT TGCAAGGAGA TAAGGGCAGT GGAAAGAAGA AGGCTCAGAG AGGCAACTCT 
ATTACAGAGA TAGTGGCAGA AGCGCAAAAG AAAAGTGTAA AACGAGCAAC TGATAAAATA 
AGTGAACGTC GTGGAACACT TCGTCAAATG CAAATGGGAA CATATGATGA ATTGGAAGAA 
GTTGAAGAAG ACGAAGATAC TGACGGAGAA ATCAGAAGAT CATCATTTGA ATATTCAGGA 
GTTGATATGA TTCAAATGTC ACATGAAAAA CAACTGGAAG ATGTGAAAAC TTGGTGGACG 
TTGTGTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nca-2 ( NM_001136363.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001129835.1 Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel (nca-2), mRNA.

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CloneID	OCa341131
Clone ID Related Accession (Same CDS sequence)	NM_001136363.2 , NM_001136363.3
Accession Version	NM_001136363.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571241600000
Organism	Caenorhabditis elegans
Product	Novel Channel type/putative Nematode CAlcium channel
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001136363.2.

ATGCTGACGC GCCGCAAATC GTCAATAGAT GGAACAAAAA CAGAAAGAAA TCGGAGAAGA 
GGAGAGTCGA TTGGTGGAGC ATTTACTGAT ATGATTAATA TTGAACCAAC GAGTATTATT 
AATAAATCGA CAGAATTACT CCACGAGAGA TTTCTCCGAG ATATGGTTCG TGTGGCTTGT 
CTTTTATCAA TGATATCATT ATGTCTTCAT ACACCAGAAA CTATCAAAAT GTGGCCACCA 
CTAAATTATA TAATTCTGGC GAATGACGTC ATTGTCACTT TGATTTTTAT TGGAGAAGCC 
GCTGTTACGA TTAATCAGAA CGGTTTATTC GATAATCAAA ACTCGTATTT ACGGGATCGA 
TGGTATCAAT TTGAATTTTT TCTTCTCATT AATCACATTC TATCATGTGT TATTCATATT 
TATGAACTTT GCTCAATATG GTTTCCAGCA CTTAATTTTG TGTATTATCC ATGGCTTGGA 
GCACTTCGAT CAGCTCGACC ATTCATTTTT CTTCGATTTA TTCGATCAAT AGTGAGGTTT 
AAACTTCCCA AAAATCGAAT TAAATTAATC ATAAAACGAT CATCTCAACA AATCCAAAAT 
GTTACAATAT TCTTCATGTT TTTTGTATTT TCGTATGCAA TAATGGGTGT TCAATTATTT 
GGACGACTCA ATTATCACTG CGTTGTCAAT GGAACTGATC CAAATAATGT TACAATTGCC 
GATCTAGCAA TCCCTGACAC AATGTGCTCA CAGAAAGGAG CTGGTGGTTA CGAGTGCCCA 
GGGAATATGG TTTGTATGAG ATTACAACTG AAACCACAAG AAGAAGGATT CTACGGACAA 
TTTAGTGATT TTGCAAGCTC TTTGTTCACA GTTTATCTGG CTGCATCTCA AGAAGGATGG 
GTTTATGTTT TATATGATTG TTTGGATTCA TTACCTTCAT TTCTTGCATT TTTCTATTTT 
GTAACGCTCA TTTTCTTTCT GGCGTGGTTG GTGAAGAATG TTTTCATCGC CGTCATTACC 
GAAACATTTG CTGAGATTCG TGTTCAATTC AGTGAAATGT GGCAGACACG TGAAGCAACA 
ACTGATCATG TGTACACACA GAAACTGGAA AAAGATGAAG ATGGATGGAA ATTGGTGGAA 
GTTGATAAAT ACAATAGAGC TCATTCTAAT AATTCACTTT TCTTGCATAC AATAGTCACT 
TCAACAGCTT TTCAAACAGT CATGCAATTG TTAATCCTTG CAAATGCAAT ATTTCATGCT 
ACTTTTGTAT TTTATCATGA TGAATCCGAT CAAATCAGGA AAATTTGGTA TTATTACGTG 
GAAGTTGGCT TTACGATACT CTTTAATACG GAAGTCATCA TAAAGATTTA TGCATTTGGA 
TGGAAAGCTT ATATTGCACG TGGGCAACAT AAATTTGATT GTATTCTTTG CGTTGGTTCA 
AGCCTCAATG CAATATGGGT TCTTTATGAA ACAAATATCT TCACATACTT CCAAGTTTTC 
CGAATTGCTC GACTTATCAA AGCCTCTCCA ATGCTAGAAG ACTTCGTTTA TAAAATCTTT 
GGACCCGGAA AGAAATTGGG AGGTCTTGTG ATATTCACTG GAATTCTTTT GATCGTTACT 
TCTGCAATTT CGTTGCAGCT ATTTTGCTAT GTGCCAAAAT TAAACAAATT TACCAATTTT 
GCTGTGGCTT TCATGTCAAT GTTCCAAATA ATAACACAAG AAGGCTGGAC AGATGTTGTC 
ATTGAAATTC TAAGAGCATG TAATGAACAA GCGGTTCCAT TTGTTGCCAT CTATTTTGTT 
GCTTATCACT TACTGGTTAC ACTGTTCGTT TTGTCACTCT TCGTTGCCGT CATTCTTGAT 
AATTTGGAAA TGGACGAAGA ATTGAAAAAA GTGAAACAAC TGAAAGCAAG AGAACAGGAT 
ACAATAAAGA CAACACTTCC GATGCGTCTC CGCATTTTCA ATCGATTTCC GACTGCTCCA 
ACAATGGTTA CAATGAAGAA AGTATCGTCA GAGTTTCCTC TTCCAAAAAT TAGAGATTCG 
TTTACAAGAC AGTTTGCGGA TGAATTCGTA GAGACAAGTG ATGATACTAT TCAGGAAATC 
GGATTTAAAG TCCGATCAAT GTTATCCGGT AGAGGACCAT CTAAAGAAAC ACGTATAACA 
ACAACAATCC GACATGTTGG ACAACTTTCA AACAAAACAA TTCTCACCTC AATGCTCACT 
GAATCAAATC GGAATCGTGC TCTTTTCTCG GAATCAAATC AACATTTGGC TAGTTTAACC 
AGAAGTAACA CTTCATCAAA ACACGGAAAA AGTGGAGCTT TATCAACGAA TTCTCGATCA 
AGAACACGTG GATTGGCTTC ATTAAAAGGG AAACATATGG TGGAAGGATT CAAAGAAAAT 
GGAGATTTAC GACCAGAAGA CACTGCTAGA AAAGTGGAGA AGCATGGGGA GATTGATTTT 
AAAGCATTGC AAATGAAAAG GGCTCATGCT GAAATTACAA GAAACCGAAT AGAAGAAGAA 
ATGCGCGAGA ACCATCCGAT GTTCGATCGT CCGCTCTTTC TTGTTGGCAG AGAATCGTCT 
CTACGTAGAA TGTGCCAACT CATTGCTCAT TCCAGACATT CATACGATCA AAATGATGGC 
CAACACAGAA AACATTCGAA TAAATATAAA CAATTTCATG ACTTTCTGGC CATCATGACA 
TATATGGATT GGACAATGGT GCTAGTAACA ACCTTATCAT GTTGCAGTAT GTTATGGGAA 
TCACCTTGGC CAACGACAGG AGAGAATCTG ATTTTTAATA ACTTTTATCT CCAAATTGCT 
GAATACATCT TTGTGCTTGT GATGAGTTTC GAACTAATTG TAAAATGCAT TGCAAATGGT 
CTCTTCTTCA CACCAAAAGC CTTGGTAACA GATGTCGGAG ATATTCTTAC GATTTTCATT 
TATATTACAA GTCTAATGTT TCTGATATGG ATGCCAAATC ACATCGAAAT CAACAGCTGG 
GCTCAGCTTC TTATGGTTTG CCGAGCAATG AGACCATTGA GAGTTTACGC GTTGATTCCG 
CATATTCGAA GAGTCGTTGT GGAGTTATGC AGAGGATTTC GGGAAATTTT ATTGGTAACA 
ATTCTTCTCG TCGTTCTCAT GTTCATTTTC GCAAGTTTCG GAGTTCAACT AGTCGGTGGA 
AAGCTAGCAT CATGCAATGA TCCAATGATA ACATCTCGTG AAAACTGTAC AGGATTGTAT 
GACGTCAAAT TGTTTGTTAC TCGGATGGAA GTTTATGGTA AAAATGACAA TCTGATGCAT 
CCTTCAATCA TTGTGCCACG TGTATGGACA AATCCACGGA ATTTCAATTT CGATCACATT 
GGGAATGCAA TGTTGGCTCT TTTTGAAACA CTTTCCTACA AGGGATGGAA TGTAGTCAGA 
GATGTTTTAT ACTTGCGACA TGGAGCTTGG GCAGTTCTCT TCATTCACAT TTATGTATTC 
ATTGGATGTA TGATTGGACT AACACTATTC GTCGGTGTGG TTGTTGCAAA TTACACTGAA 
AACCGTGGAA CAGCCCTTCT AACAGTTGAT CAACGTCGTT GGCATGATCT GAAGGCTCGT 
CTGAAAATGG CTCAACCTCT ACATGTTCCA CCGAAACCTC CGGAATCTGC AAAACTGCGT 
TGTTACCTCT ATGATCTGAC GACGTCTCGA TGGTTCAAAC AGTTATTTGC TGTTCTTGTG 
GTTGTCAATT CATTTACACT TGTTATTCCA TGGAATGTTA GTGAAGAACA GGATAGAAAG 
ACTTTTCTGT TATGTCTTAC TGTCATTTCT GCAATTTGCA ATATTTTATT CACTTTGGAA 
TGCTTGCTGA AAATGATTGC GTTCACGTTA TCGGGATTTT GGCAATCAAG AAGAAATCGA 
ATTGATTTTA TTATCACTAT CCTCGGAATC AATTGGATTG TTTTTCATTT TTTGTTTCAA 
TTACCTGCGT ACTTTGCTGG TGGAATAACC GAATGGAAGC GTCTCACATA CACTTATGGA 
TATCTCGTCG TTATACTCCG TTTTTTCACA ATTGCCGGCC GTAAATCCAC ACTGAAAATG 
TTAATGTTAA CAGTTGTAAT GTCAATGGTT CGATCATCAT TTATTATTGC TGCAATGTTT 
CTATTGGTTC TTTTTTATGC AAATGCAGGA GTTGTGTTAT TTGGAATGGT CAAATATGGA 
CAAGCTGTTG GAAAACATGT AAATTTCCGA AATGGACGAG AGGCATTGGT GGTGTTATTT 
CGATCAGTTA CCGGTGAAGA TTGGAATGAT ATTATGCACG ATTGTATGAG AGCACCACCA 
TTTTGCAATT GGCATCCAGG GCTCAGCTAT TGGCAAACTG ATTGTGGGAA TTATGTTGGA 
GCAATTGTTT ATTTTTGCTC CTTCTATCTC ATTATCACAT ATATTGTGCT CAATCTTCTT 
GTCGCTATCA TCATGGAGAA CTTCTCATTG TTCTACTCAT CTGAAGAAGA TGCACTTCTC 
TCGTATGCCG ATATTCGAAA TTTCCAACTT GTTTGGAATA TGGTGGATAT TGAACAGAAA 
CGATCAATTC CAGTGAGACG TGTCAAATTT CTGTTGAGAT TGTTAAAAGG AAGACTGGAA 
GTTAATGATG AATCCGATGG ATTGTTGTTT AAACATATGT GTCATGAAAT GGAACGATTG 
CATAATGGAG ATGATGTTTC ATTTCATGAT GTTCTCAACA TGCTCTCGTA TCGAAGTGTG 
GATATTCGAA AAAGTTTACA ACTGGAAGAA TTACTTCAAA GAGAAGAACT TGAATACATA 
ATTGAAGAAG AAGTTGCAAA GCACACTATT CGGGCGTGGC TAGAGAATTG TTTGAAAAAC 
ATCAAAGCCA AGCAGAATAA TACTCTTGGA AAAATGAGCT CAATTGGGTC AACATTTGCA 
TTTCCACAAT CTCAAGAAGT ATTAACAAAA GGAGTTGTGC TCACGGAGGC TTCTCCTGAA 
GAAGATTCAT TGCAAGGAGA TAAGGGCAGT GGAAAGAAGA AGGCTCAGAG AGGCAACTCT 
ATTACAGAGA TAGTGGCAGA AGCGCAAAAG AAAAGTGTAA AACGAGCAAC TGATAAAATA 
AGTGAACGTC GTGGAACACT TCGTCAAATG CAAATGGGAA CATATGATGA ATTGGAAGAA 
GTTGAAGAAG ACGAAGATAC TGACGGAGAA ATCAGAAGAT CATCATTTGA ATATTCAGGA 
GTTGATATGA TTCAAATGTC ACATGAAAAA CAACTGGAAG ATGTGAAAAC TTGGTGGACG 
TTGTGTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129835.1
CDS	39..5330
Translation	MLTRRKSSIDGTKTERNRRRGESIGGAFTDMINIEPTSIINKSTELLHERFLRDMVRVACLLSMISLCLHTPETIKMWPPLNYIILANDVIVTLIFIGEAAVTINQNGLFDNQNSYLRDRWYQFEFFLLINHILSCVIHIYELCSIWFPALNFVYYPWLGALRSARPFIFLRFIRSIVRFKLPKNRIKLIIKRSSQQIQNVTIFFMFFVFSYAIMGVQLFGRLNYHCVVNGTDPNNVTIADLAIPDTMCSQKGAGGYECPGNMVCMRLQLKPQEEGFYGQFSDFASSLFTVYLAASQEGWVYVLYDCLDSLPSFLAFFYFVTLIFFLAWLVKNVFIAVITETFAEIRVQFSEMWQTREATTDHVYTQKLEKDEDGWKLVEVDKYNRAHSNNSLFLHTIVTSTAFQTVMQLLILANAIFHATFVFYHDESDQIRKIWYYYVEVGFTILFNTEVIIKIYAFGWKAYIARGQHKFDCILCVGSSLNAIWVLYETNIFTYFQVFRIARLIKASPMLEDFVYKIFGPGKKLGGLVIFTGILLIVTSAISLQLFCYVPKLNKFTNFAVAFMSMFQIITQEGWTDVVIEILRACNEQAVPFVAIYFVAYHLLVTLFVLSLFVAVILDNLEMDEELKKVKQLKAREQDTIKTTLPMRLRIFNRFPTAPTMVTMKKVSSEFPLPKIRDSFTRQFADEFVETSDDTIQEIGFKVRSMLSGRGPSKETRITTTIRHVGQLSNKTILTSMLTESNRNRALFSESNQHLASLTRSNTSSKHGKSGALSTNSRSRTRGLASLKGKHMVEGFKENGDLRPEDTARKVEKHGEIDFKALQMKRAHAEITRNRIEEEMRENHPMFDRPLFLVGRESSLRRMCQLIAHSRHSYDQNDGQHRKHSNKYKQFHDFLAIMTYMDWTMVLVTTLSCCSMLWESPWPTTGENLIFNNFYLQIAEYIFVLVMSFELIVKCIANGLFFTPKALVTDVGDILTIFIYITSLMFLIWMPNHIEINSWAQLLMVCRAMRPLRVYALIPHIRRVVVELCRGFREILLVTILLVVLMFIFASFGVQLVGGKLASCNDPMITSRENCTGLYDVKLFVTRMEVYGKNDNLMHPSIIVPRVWTNPRNFNFDHIGNAMLALFETLSYKGWNVVRDVLYLRHGAWAVLFIHIYVFIGCMIGLTLFVGVVVANYTENRGTALLTVDQRRWHDLKARLKMAQPLHVPPKPPESAKLRCYLYDLTTSRWFKQLFAVLVVVNSFTLVIPWNVSEEQDRKTFLLCLTVISAICNILFTLECLLKMIAFTLSGFWQSRRNRIDFIITILGINWIVFHFLFQLPAYFAGGITEWKRLTYTYGYLVVILRFFTIAGRKSTLKMLMLTVVMSMVRSSFIIAAMFLLVLFYANAGVVLFGMVKYGQAVGKHVNFRNGREALVVLFRSVTGEDWNDIMHDCMRAPPFCNWHPGLSYWQTDCGNYVGAIVYFCSFYLIITYIVLNLLVAIIMENFSLFYSSEEDALLSYADIRNFQLVWNMVDIEQKRSIPVRRVKFLLRLLKGRLEVNDESDGLLFKHMCHEMERLHNGDDVSFHDVLNMLSYRSVDIRKSLQLEELLQREELEYIIEEEVAKHTIRAWLENCLKNIKAKQNNTLGKMSSIGSTFAFPQSQEVLTKGVVLTEASPEEDSLQGDKGSGKKKAQRGNSITEIVAEAQKKSVKRATDKISERRGTLRQMQMGTYDELEEVEEDEDTDGEIRRSSFEYSGVDMIQMSHEKQLEDVKTWWTLCD

Target ORF information:

RefSeq Version	NM_001136363.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel (nca-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136363.3

ORF Insert Sequence:

ATGCTGACGC GCCGCAAATC GTCAATAGAT GGAACAAAAA CAGAAAGAAA TCGGAGAAGA 
GGAGAGTCGA TTGGTGGAGC ATTTACTGAT ATGATTAATA TTGAACCAAC GAGTATTATT 
AATAAATCGA CAGAATTACT CCACGAGAGA TTTCTCCGAG ATATGGTTCG TGTGGCTTGT 
CTTTTATCAA TGATATCATT ATGTCTTCAT ACACCAGAAA CTATCAAAAT GTGGCCACCA 
CTAAATTATA TAATTCTGGC GAATGACGTC ATTGTCACTT TGATTTTTAT TGGAGAAGCC 
GCTGTTACGA TTAATCAGAA CGGTTTATTC GATAATCAAA ACTCGTATTT ACGGGATCGA 
TGGTATCAAT TTGAATTTTT TCTTCTCATT AATCACATTC TATCATGTGT TATTCATATT 
TATGAACTTT GCTCAATATG GTTTCCAGCA CTTAATTTTG TGTATTATCC ATGGCTTGGA 
GCACTTCGAT CAGCTCGACC ATTCATTTTT CTTCGATTTA TTCGATCAAT AGTGAGGTTT 
AAACTTCCCA AAAATCGAAT TAAATTAATC ATAAAACGAT CATCTCAACA AATCCAAAAT 
GTTACAATAT TCTTCATGTT TTTTGTATTT TCGTATGCAA TAATGGGTGT TCAATTATTT 
GGACGACTCA ATTATCACTG CGTTGTCAAT GGAACTGATC CAAATAATGT TACAATTGCC 
GATCTAGCAA TCCCTGACAC AATGTGCTCA CAGAAAGGAG CTGGTGGTTA CGAGTGCCCA 
GGGAATATGG TTTGTATGAG ATTACAACTG AAACCACAAG AAGAAGGATT CTACGGACAA 
TTTAGTGATT TTGCAAGCTC TTTGTTCACA GTTTATCTGG CTGCATCTCA AGAAGGATGG 
GTTTATGTTT TATATGATTG TTTGGATTCA TTACCTTCAT TTCTTGCATT TTTCTATTTT 
GTAACGCTCA TTTTCTTTCT GGCGTGGTTG GTGAAGAATG TTTTCATCGC CGTCATTACC 
GAAACATTTG CTGAGATTCG TGTTCAATTC AGTGAAATGT GGCAGACACG TGAAGCAACA 
ACTGATCATG TGTACACACA GAAACTGGAA AAAGATGAAG ATGGATGGAA ATTGGTGGAA 
GTTGATAAAT ACAATAGAGC TCATTCTAAT AATTCACTTT TCTTGCATAC AATAGTCACT 
TCAACAGCTT TTCAAACAGT CATGCAATTG TTAATCCTTG CAAATGCAAT ATTTCATGCT 
ACTTTTGTAT TTTATCATGA TGAATCCGAT CAAATCAGGA AAATTTGGTA TTATTACGTG 
GAAGTTGGCT TTACGATACT CTTTAATACG GAAGTCATCA TAAAGATTTA TGCATTTGGA 
TGGAAAGCTT ATATTGCACG TGGGCAACAT AAATTTGATT GTATTCTTTG CGTTGGTTCA 
AGCCTCAATG CAATATGGGT TCTTTATGAA ACAAATATCT TCACATACTT CCAAGTTTTC 
CGAATTGCTC GACTTATCAA AGCCTCTCCA ATGCTAGAAG ACTTCGTTTA TAAAATCTTT 
GGACCCGGAA AGAAATTGGG AGGTCTTGTG ATATTCACTG GAATTCTTTT GATCGTTACT 
TCTGCAATTT CGTTGCAGCT ATTTTGCTAT GTGCCAAAAT TAAACAAATT TACCAATTTT 
GCTGTGGCTT TCATGTCAAT GTTCCAAATA ATAACACAAG AAGGCTGGAC AGATGTTGTC 
ATTGAAATTC TAAGAGCATG TAATGAACAA GCGGTTCCAT TTGTTGCCAT CTATTTTGTT 
GCTTATCACT TACTGGTTAC ACTGTTCGTT TTGTCACTCT TCGTTGCCGT CATTCTTGAT 
AATTTGGAAA TGGACGAAGA ATTGAAAAAA GTGAAACAAC TGAAAGCAAG AGAACAGGAT 
ACAATAAAGA CAACACTTCC GATGCGTCTC CGCATTTTCA ATCGATTTCC GACTGCTCCA 
ACAATGGTTA CAATGAAGAA AGTATCGTCA GAGTTTCCTC TTCCAAAAAT TAGAGATTCG 
TTTACAAGAC AGTTTGCGGA TGAATTCGTA GAGACAAGTG ATGATACTAT TCAGGAAATC 
GGATTTAAAG TCCGATCAAT GTTATCCGGT AGAGGACCAT CTAAAGAAAC ACGTATAACA 
ACAACAATCC GACATGTTGG ACAACTTTCA AACAAAACAA TTCTCACCTC AATGCTCACT 
GAATCAAATC GGAATCGTGC TCTTTTCTCG GAATCAAATC AACATTTGGC TAGTTTAACC 
AGAAGTAACA CTTCATCAAA ACACGGAAAA AGTGGAGCTT TATCAACGAA TTCTCGATCA 
AGAACACGTG GATTGGCTTC ATTAAAAGGG AAACATATGG TGGAAGGATT CAAAGAAAAT 
GGAGATTTAC GACCAGAAGA CACTGCTAGA AAAGTGGAGA AGCATGGGGA GATTGATTTT 
AAAGCATTGC AAATGAAAAG GGCTCATGCT GAAATTACAA GAAACCGAAT AGAAGAAGAA 
ATGCGCGAGA ACCATCCGAT GTTCGATCGT CCGCTCTTTC TTGTTGGCAG AGAATCGTCT 
CTACGTAGAA TGTGCCAACT CATTGCTCAT TCCAGACATT CATACGATCA AAATGATGGC 
CAACACAGAA AACATTCGAA TAAATATAAA CAATTTCATG ACTTTCTGGC CATCATGACA 
TATATGGATT GGACAATGGT GCTAGTAACA ACCTTATCAT GTTGCAGTAT GTTATGGGAA 
TCACCTTGGC CAACGACAGG AGAGAATCTG ATTTTTAATA ACTTTTATCT CCAAATTGCT 
GAATACATCT TTGTGCTTGT GATGAGTTTC GAACTAATTG TAAAATGCAT TGCAAATGGT 
CTCTTCTTCA CACCAAAAGC CTTGGTAACA GATGTCGGAG ATATTCTTAC GATTTTCATT 
TATATTACAA GTCTAATGTT TCTGATATGG ATGCCAAATC ACATCGAAAT CAACAGCTGG 
GCTCAGCTTC TTATGGTTTG CCGAGCAATG AGACCATTGA GAGTTTACGC GTTGATTCCG 
CATATTCGAA GAGTCGTTGT GGAGTTATGC AGAGGATTTC GGGAAATTTT ATTGGTAACA 
ATTCTTCTCG TCGTTCTCAT GTTCATTTTC GCAAGTTTCG GAGTTCAACT AGTCGGTGGA 
AAGCTAGCAT CATGCAATGA TCCAATGATA ACATCTCGTG AAAACTGTAC AGGATTGTAT 
GACGTCAAAT TGTTTGTTAC TCGGATGGAA GTTTATGGTA AAAATGACAA TCTGATGCAT 
CCTTCAATCA TTGTGCCACG TGTATGGACA AATCCACGGA ATTTCAATTT CGATCACATT 
GGGAATGCAA TGTTGGCTCT TTTTGAAACA CTTTCCTACA AGGGATGGAA TGTAGTCAGA 
GATGTTTTAT ACTTGCGACA TGGAGCTTGG GCAGTTCTCT TCATTCACAT TTATGTATTC 
ATTGGATGTA TGATTGGACT AACACTATTC GTCGGTGTGG TTGTTGCAAA TTACACTGAA 
AACCGTGGAA CAGCCCTTCT AACAGTTGAT CAACGTCGTT GGCATGATCT GAAGGCTCGT 
CTGAAAATGG CTCAACCTCT ACATGTTCCA CCGAAACCTC CGGAATCTGC AAAACTGCGT 
TGTTACCTCT ATGATCTGAC GACGTCTCGA TGGTTCAAAC AGTTATTTGC TGTTCTTGTG 
GTTGTCAATT CATTTACACT TGTTATTCCA TGGAATGTTA GTGAAGAACA GGATAGAAAG 
ACTTTTCTGT TATGTCTTAC TGTCATTTCT GCAATTTGCA ATATTTTATT CACTTTGGAA 
TGCTTGCTGA AAATGATTGC GTTCACGTTA TCGGGATTTT GGCAATCAAG AAGAAATCGA 
ATTGATTTTA TTATCACTAT CCTCGGAATC AATTGGATTG TTTTTCATTT TTTGTTTCAA 
TTACCTGCGT ACTTTGCTGG TGGAATAACC GAATGGAAGC GTCTCACATA CACTTATGGA 
TATCTCGTCG TTATACTCCG TTTTTTCACA ATTGCCGGCC GTAAATCCAC ACTGAAAATG 
TTAATGTTAA CAGTTGTAAT GTCAATGGTT CGATCATCAT TTATTATTGC TGCAATGTTT 
CTATTGGTTC TTTTTTATGC AAATGCAGGA GTTGTGTTAT TTGGAATGGT CAAATATGGA 
CAAGCTGTTG GAAAACATGT AAATTTCCGA AATGGACGAG AGGCATTGGT GGTGTTATTT 
CGATCAGTTA CCGGTGAAGA TTGGAATGAT ATTATGCACG ATTGTATGAG AGCACCACCA 
TTTTGCAATT GGCATCCAGG GCTCAGCTAT TGGCAAACTG ATTGTGGGAA TTATGTTGGA 
GCAATTGTTT ATTTTTGCTC CTTCTATCTC ATTATCACAT ATATTGTGCT CAATCTTCTT 
GTCGCTATCA TCATGGAGAA CTTCTCATTG TTCTACTCAT CTGAAGAAGA TGCACTTCTC 
TCGTATGCCG ATATTCGAAA TTTCCAACTT GTTTGGAATA TGGTGGATAT TGAACAGAAA 
CGATCAATTC CAGTGAGACG TGTCAAATTT CTGTTGAGAT TGTTAAAAGG AAGACTGGAA 
GTTAATGATG AATCCGATGG ATTGTTGTTT AAACATATGT GTCATGAAAT GGAACGATTG 
CATAATGGAG ATGATGTTTC ATTTCATGAT GTTCTCAACA TGCTCTCGTA TCGAAGTGTG 
GATATTCGAA AAAGTTTACA ACTGGAAGAA TTACTTCAAA GAGAAGAACT TGAATACATA 
ATTGAAGAAG AAGTTGCAAA GCACACTATT CGGGCGTGGC TAGAGAATTG TTTGAAAAAC 
ATCAAAGCCA AGCAGAATAA TACTCTTGGA AAAATGAGCT CAATTGGGTC AACATTTGCA 
TTTCCACAAT CTCAAGAAGT ATTAACAAAA GGAGTTGTGC TCACGGAGGC TTCTCCTGAA 
GAAGATTCAT TGCAAGGAGA TAAGGGCAGT GGAAAGAAGA AGGCTCAGAG AGGCAACTCT 
ATTACAGAGA TAGTGGCAGA AGCGCAAAAG AAAAGTGTAA AACGAGCAAC TGATAAAATA 
AGTGAACGTC GTGGAACACT TCGTCAAATG CAAATGGGAA CATATGATGA ATTGGAAGAA 
GTTGAAGAAG ACGAAGATAC TGACGGAGAA ATCAGAAGAT CATCATTTGA ATATTCAGGA 
GTTGATATGA TTCAAATGTC ACATGAAAAA CAACTGGAAG ATGTGAAAAC TTGGTGGACG 
TTGTGTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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nca-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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