Bm1_43145 cDNA ORF clone, Brugia malayi

Gene
Clones
gRNAs

Summary

Gene Symbol	Bm1_43145
Entrez Gene ID	6103514
Full Name	U5 small nuclear ribonucleoprotein 200 kDa helicase, putative
Gene Type	protein-coding
Organism	Brugia malayi

mRNA and Protein(s)

mRNA	Protein	Name
XM_001900056.1	XP_001900091.1	U5 small nuclear ribonucleoprotein 200 kDa helicase, putative

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Draft genome of the filarial nematode parasite Brugia malayi.
Ghedin E, Wang S, Spiro D, Caler E, Zhao Q, Crabtree J, Allen JE, Delcher AL, Guiliano DB, Miranda-Saavedra D, Angiuoli SV, Creasy T, Amedeo P, Haas B, El-Sayed NM, Wortman JR, Feldblyum T, Tallon L, Schatz M, Shumway M, Koo H, Salzberg SL, Schobel S, Pertea M, Pop M, White O, Barton GJ, Carlow CK, Crawford MJ, Daub J, Dimmic MW, Estes CF, Foster JM, Ganatra M, Gregory WF, Johnson NM, Jin J, Komuniecki R, Korf I, Kumar S, Laney S, Li BW, Li W, Lindblom TH, Lustigman S, Ma D, Maina CV, Martin DM, McCarter JP, McReynolds L, Mitreva M, Nutman TB, Parkinson J, Peregrín-Alvarez JM, Poole C, Ren Q, Saunders L, Sluder AE, Smith K, Stanke M, Unnasch TR, Ware J, Wei AD, Weil G, Williams DJ, Zhang Y, Williams SA, Fraser-Liggett C, Slatko B, Blaxter ML, Scott AL
Science (New York, N.Y.)317(5845)1756-60(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Bm1_43145 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bm1_43145 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBd06503	XM_001900056.1 Latest version!	Brugia malayi U5 small nuclear ribonucleoprotein 200 kDa helicase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bm1_43145 ( XM_001900056.1 ) cDNA ORF clone, Brugia malayi -> XP_001900091.1 Brugia malayi U5 small nuclear ribonucleoprotein 200 kDa helicase partial mRNA.

Price & Availability↑

CloneID	OBd06503
Clone ID Related Accession (Same CDS sequence)	XM_001900056.1
Accession Version	XM_001900056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1206892800000
Organism	Brugia malayi
Product	U5 small nuclear ribonucleoprotein 200 kDa helicase, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001893026). COMPLETENESS: incomplete on the 3' end.

ATGGCCGACG AAATAGCTCG CGTGCAACAG TACGAGTATC GACAGAATAG TAACCTTGTG 
CTGCAAGTCG ATTTCAATTT CACAGATCGT CGTGGTCGCG ATGAACCTAC CGGTGAAGTT 
ATGCCACTCA CTAAGGAGGT TTTGGCTGGT GTTAAAATGG GCGACAAGTA TCAGCGAGCC 
AAAGCACCTG CAATTGAYAC TAAAAAAGCA AGAAAAAAAA AGCGAACTGA TGCAAGTGTC 
ACAGGGAATA GTACGACTTC GCACACTTTG TTGGGAGATT CAAATACGGA ACTRATGGGC 
TTGTACAAGC CAAGAACGCA AGAAACAAGG CAAACCTATG AAGCAATATT GGCTTATATA 
CAAGATGCCA TCGGAGATCA GCCACGCGAT ATTCTGTGTG GTGCAGCTGA TGAAGTCTTA 
GCAGTATTGA AGTCAGACAA AATTCGTGAG AAAGATCGTA AGAAGGAAGT AGAACTTCTT 
CTTGGATTGT TGACGGATGA AAGAATAGCT GTATTGATTA ATCTTGCCAA GAAAATAACA 
GATTTTTCAG TCGATGACGA AAAAGGGAAA TTTGACAATC AAGATGAAAT AGATGAAACA 
ATCGGTGTAA ATGTACAGTT CGATGATTCT GATGAGGAAG CTGAGGGCGA CGAAGGTATG 
GAGGAAGAAG TAAAAGAAGA AGGTTCAGCT GGGGAAGATG AAGGTGGTGT TGAAGCTGTT 
TTTGAAGAAA CACTTAAGGC TCGATATGTT GATGAGGACG ATGAGAATGC GGGTGGTGAA 
CGAAAAGGAA ATCTGCATGC TCGCGATATA GATGCGCATT GGATTCAGCG GTCACTCTCA 
AAATTTTACA AAGATCCAAT TGTGGCACAG CAGAAAGTTA ATGAAGTGCT GCAAGTTTTG 
AAGGAGGCAT CCGATGACCG TGATTGTGAG AATCAGCTGG TATTATTGCT TGGTTTTGAT 
CAGTTTGAAT TTATCAAAGT GCTTCGCCAG CATCGCCAGA TGATTCTGTA CTGTACTCTC 
TTGAAACAGG CACAAGAAGG TAAAGAAAGG GAAAATATCG AGAAAGAAAT GCTGAGCCGG 
CCGGAATTAC ATCATATTCT TGCTGAACTT CACGAGACAG AAAGTGCAGA TACAGTTGAG 
GCCGAACGGG AAAAGCGTGC ACGAGTACAG CAGCAGCGAA GAATAGCTGA AGCTGAAGGG 
GGTAATGATG AGGGAACAGC AGTGGGTAAC TGGTTACAAT CACGTAAACG TTGTCATTTG 
CCAGATGGCT CGTACCGTAA GCAGAAAAAA AGTTATGAAG AAGTTCATGT ACCGGCGCTT 
AAACCAAAGC CATTCGAGGA AAATGAGAAA CTGGTTGCAA TAACGGACTT GCCAAAATAT 
GCACAGCCAG CTTTCGAAGG ATTCAAGACA CTTAATCGAG TYCAGTCGAG ACTATGCGAT 
TCTGCTTTAA AATCTGATGA ACACTTGCTT TTATGTGCAC CTACGGGCGC TGGTAAAACA 
AATGTTGCTT TACTGTGCAT TTTGCGCGAG ATATCAAAAC ATATGAATGC TGATGGGAGC 
ATACGAATAG ATGAATTTAA ATGCATCTAC ATTGCTCCAA TGAAATCATT AGTACAGGAA 
ATGGTCGGAA CTTTCACAAA ACGCTTAGCA CCTTACAAAA TTGCTGTTGG TGAAATGACT 
GGTGATCAGC AGATGAACAA GGAACAATTC ATGCAAACTC AAGTGATTGT TTGCACGCCT 
GAGAAATACG ATATTGTGAC TCGTAAAGGT GGCGAACGTG CATACAGTCA GTTAGTTGCA 
CTGTTAATTA TTGATGAAAT TCATCTTTTG CACGACAACC GCGGACCAGT GCTAGAAGCC 
ATTGTCGTAA GAACTCTACG ACAAATGGAA CAAAACCATG AAGAATGTCG TTTAGTCGGA 
CTTTCAGCAA CTTTACCAAA CTATCAAGAT GTTGGAACAT TCCTGCGAGT GAAGCCGCAA 
AATTTGTTTT TCTTTGATAA CAGCTTTAGA CCTGTTCCGC TTGAACAGCA GTATATCGGT 
ATTACGGAGA AGAAAGCGTT AAAACGATAT CAAGCAATGA ATGAAGTCGT TTATGACAAA 
GTAATGGAGT ATGCGGGAAA GAGCCAGGTT CTAATTTTTG TACACTCACG GAAGGAAACA 
GCGAAAACAG CAAAGGCTAT ACGTGATGCC TGTCTTGAAA AGGATACTTT AAGTGCATTC 
ATGCGAGAGG GTAGTGCAAG TACTGAAATT CTCCGGAGTG AAGCATCGCA GGTGGCAAAT 
GCGGATTTGA GGGACTTGAT TCCTTATGGT TTTGCAATTC ATCATGCAGG TATGACTCGC 
GTTGACCGTA CCCTTGTTGA GGACTTGTTC GCCGATCGCC ACTTGCAGGT ACTTGTTTCA 
ACAGCTACTC TGGCCTGGGG TGTTAACTTA CCAGCTCATA CCGTGATCAT CAAAGGAACA 
CAAATTTACA GTCCAGAAAT TGGACGTTGG ACTGAATTGG GAGCTTTAGA TGTTATGCAG 
ATGCTTGGAC GTGCTGGTCG ACCTCAGTAT GATTCGAAAG GCAAGGGTAT TATGATAACG 
CATCACTCCG AGTTGCAATA CTATYTATCA CTCATGAATC AACAGTTGCC GATCGAAAGT 
CAGTTAATCT CAAAATTAGC TGATACGCTA AACGCCGAAA TAGTATTGGG AACTATCAAC 
AATGTCAGTG ATGCTATGAA TTGGTTGGGC TATACATATT TGTATGTTCG CATGGTTAAG 
TCACCAACAC TGTATGGGAT TTCACATGAA CAAACGAAAC TGGATCCTTT ACTGGAACAG 
CGTCGTGCAG ATCTGATTCA TACAGCTGCA GCRCAATTGG ATAAAGCTAG TCTTATCAAA 
TATGATCGTA GAAGTGGACT TATCCAAGCA ACCGAATTGG GTCGCATTGC TTCTCACTTT 
TATTGCACTC ATGATACGAT GCAAACATAT AATCAGCTGT TGAAGGCAAC AGCAACTGAA 
ATTGACTTGT TTCGTATATT TTCAATGTCA TCAGAATTCC GGAATATCAT GGTCCGTGAA 
GAGGAGAAGC TAGAGTTACA GAAACTGGCT GAACATGTTC CAGTTCCAAT CAAAGAAAGT 
TTAGAAGAGA GCAGTGCGAA AGTTAATGTA CTTCTACAGG CTTACATCTC ACAACTGAAG 
TTGGAAGGGT TCGCGCTTCA AAGTGATATG GTATTTATTA GTCAGTCGGC AGGACGACTG 
TTTCGAGCAC TGTTTGAAAT CGTATTATGG AGAGGTTGGG CTCATCTCGC ACAGGCACAA 
AGTTCTTGGC GTTTGCAAAA TGATAAGTGC TCGACAGTGG CAGTTGTACG AAGCATCGAT 
AAGAAGAACC TTTCATTTGA ACGCTTATAT GATCTTGACC AACATCAGCT GGGCGAGCTG 
GTGAAAATGC CAAAAATGGG GAAGCCCTTG TACAAATTCA TCCGCCAGTT ACCGAAATTA 
GATATGACAG CATTAATCCA ACCGATAACG CGATCGACTT TAAGAATTGA ACTGACAATT 
ACTCCCGATT TTCTGTGGGA TGCTCGTGTA CATGGAACGG CTGAAGGATT TTGGATATTT 
GTTGAAGATG TTGATGGAGA GCTGATTCTA CATCATGAAT ATTTCCTTTT GAAGCAGAAG 
TTTTGCGAAG ATGAACATTT GGTAAAGATG TTCGTACCGG TCTTCGATCC GCTGCCGCCA 
TTATATTTTA TACGCATAGT TTCCGATCGT TGGCTTGGTA GTGAAACTGT TTTGCCGATA 
TCTTTTCGAC ACTTGATTTT GCCCGAGAAA TATCCACCGC CAACCGAATT ACTGGATTTG 
CAACCATTAC CTTTATCAGC CTTGAACAGC AAGCTTTTCC AGTCAGTATT CGAACAAAAA 
AACATCTCAG TATTCAATCC CATTCAAACA CAAGAACGAA ATAAAACTAG AGCGGATTTT 
CGAAATACGA TTTATGCAGG CACAAATTGT GGCTTCACTT CCTTAYTGAT CAAATCAGGA 
AATTTGATTT ATTTTTTGTT TCAGTTTTTC SGAACGGTTT ATGAAGGTAA CGATAATGTA 
TTTATTGGTG CACCACATGG AAGTGGTAAA ACTGTTTGTG CCGAGTTCGC TATTCTTAGA 
CACTTTGATA ATAGGCCTGA CGCGAAAGCT GTTTATGTGA CGCCAATGGA AGATTTAGCT 
GAAAAAAAGT TCACCGACTG GCAGGACCGA ATTGGAACTG CACTAGAAAA AACAGTTGTT 
ATGTTAACGG GTGAGCCATC GACTGATCTG AAGTTACTAC AGCGAGGACA GTTGATCATA 
GCAACACCAG AAAAGTGGGA CAATGTTAGT AGACGTTGGA AACAGCGAAA GAATGTTCAA 
GCTGTGCGGT TATTTATTGT TGACGATCTT CATATGATTG GAGGCAGTAA TGGGCCGGTA 
CTGGAAGTAA TTTGCTCACG CATGCGTTAC ATGTCGTCAC AGCTGGATTC AACAGTTCGA 
ATTGTTGCTC TTTCATCATC ACTAGCTAAT GCCAGAGACG GTTTTAACTT ATCTCACACT 
GCTTCTCGGC TGGCTGCAAT GGCACGACCG GTTTATGCAG CAGTCGTCCG ACATGGTGGC 
AAGTTGCGTC CACGCCCTGC ACTCGTTTTT GTGCCTAGTA GACGGCAGTC GAGATCAACT 
GCTGTCGATA TGCTTACAAT GGCACATGCT GATGGACAGT CGAAGCGGTT TTTGCATATT 
AATCCACAAG AACCAAGTTT TATTCGATTG CTCGATAATG TTCAGGATAA AACTCTAAAA 
GAAACATTAT CTTGTGGAGT AGGTTTTCTA CATGAAGGTA CAGGTGTTAG AGATATGGCC 
GTTGTGGAAC AGCTTTTTCA AAGTGGCGCA ATTCAGGTAT GCATCGTTCC GCGCACGATG 
TGCTATCAAA TTTCCATGAG TGCGTATGTC GTAATCATTA TGGACACACA ATTTTATAAT 
GGAAAGTACC ATGTCTATGA AGATTATCCA ATAGGCGACG TTTTGCACAT GGTTGGACTA 
GCGAATAGAC CCGGGATTGA TGACGATGCA AAATGTGTCC TCATGTGTCA GTCTTCTAAA 
AAGGATTTTT TCAAAAAGTT CCTTTATGAA CCACTTCCTG TTGAGTCACA TTTGGATCAT 
TGTCTTCATG ATCACTTTAA TGCCGAAATT GTGACTAAAA CAATCGAAAA TAAACAGGAC 
GCAATCGACT ACCTTACATG GACTCTGTTA TATCGACGTA TGACACAGAA TCCAAATTAT 
TACAATTTGC AAGGTGTGAC GCATCGTCAT TTATCAGACA GTCTTTCCGA ACTAGTAGAA 
AATACGCTGA AAGATCTGGA AAATTCGAAA TGCATTACAA TCAAGAACGA TATGGACACA 
CAGCCACTTA ATTTAGGAAT GATTGCAGCC TACTACTACA TAAGTTATAC AACAATCGAA 
GTATTCAGCA TGTCTTTGAG TGCGAAAACT AAGCTGCGAG CTCTTATTGA AATTATCAGT 
AATGCAAGTG AATTTGCAAA TATGCCTATA CGTTACAAAG AAGATATTTT GTTGAAACAA 
CTGGCTGACC GATTATCAAG CCAGCAGAAA CACCAGAAAT TTACAGATCC ACATGTTAAG 
GTCAATTTAT TAATGAATGC TCACTTGGCT CGAATACAAT TGTCAGCTGA ACTGAATAAA 
GATACAGAAG TTATCATTCT TAGAGCAATC CGTCTAGTGC AAGCATGTGT TGATGTTTTA 
AGCAGCAATG GTTGGTTAAT GCCTGCAATT CATGCAATGG AACTGTCACA AATGTTAACA 
CAAGCGATGT TCACCAGTGA ATCATATTTG AAACAACTAC CCCATTGCTC AACTTCATTA 
CTTGAACGAT GCAAAGAAAA AAAAATATCA TCAATATTTG ATTTACTGGA TTTGGAAGAT 
GACGTCCGAC AGACACTTTT ACAAATGACT CCAGCAGAAA TGTCGGATGT TGCTCGTTTT 
TGCAATCATT ATCCTAGCAT TGAAGTTGAA CACAAAATTG AAAATAATGG AACCATTACT 
GTGGGTGATA CAGTGAATGT TACCGTAGAA ATGGAACGTG AAAATGATTT GAATGGAATG 
GCTCCACCTG TAGTAGCACC CCTTTTTCCG CAGAAGCGGA AGGAAGAAGG GTGGTGGTTG 
GTTATTGGGG ATCATTCTTC TAATGCATTG TTTTCCATAA AAAGGTTAAC AGTCCATCAG 
AAAGCAAAAA TGACGCTTGA TTTTACTGCA TTGGCAGTAG GGAAGATGCA TTATAAGCTA 
TACTTCATTT GTGATTCATA CCTAGGAGCT GACCAAGAAT TCGATCTCAA ATTTCGTGTC 
GAAGAAACTG GACGTAGTCG AAAGCGAGTT CGTGACGATG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001900091.1
CDS	47..6451
Translation	MADEIARVQQYEYRQNSNLVLQVDFNFTDRRGRDEPTGEVMPLTKEVLAGVKMGDKYQRAKAPAInullTKKARKKKRTDASVTGNSTTSHTLLGDSNTEnullMGLYKPRTQETRQTYEAILAYIQDAIGDQPRDILCGAADEVLAVLKSDKIREKDRKKEVELLLGLLTDERIAVLINLAKKITDFSVDDEKGKFDNQDEIDETIGVNVQFDDSDEEAEGDEGMEEEVKEEGSAGEDEGGVEAVFEETLKARYVDEDDENAGGERKGNLHARDIDAHWIQRSLSKFYKDPIVAQQKVNEVLQVLKEASDDRDCENQLVLLLGFDQFEFIKVLRQHRQMILYCTLLKQAQEGKERENIEKEMLSRPELHHILAELHETESADTVEAEREKRARVQQQRRIAEAEGGNDEGTAVGNWLQSRKRCHLPDGSYRKQKKSYEEVHVPALKPKPFEENEKLVAITDLPKYAQPAFEGFKTLNRnullQSRLCDSALKSDEHLLLCAPTGAGKTNVALLCILREISKHMNADGSIRIDEFKCIYIAPMKSLVQEMVGTFTKRLAPYKIAVGEMTGDQQMNKEQFMQTQVIVCTPEKYDIVTRKGGERAYSQLVALLIIDEIHLLHDNRGPVLEAIVVRTLRQMEQNHEECRLVGLSATLPNYQDVGTFLRVKPQNLFFFDNSFRPVPLEQQYIGITEKKALKRYQAMNEVVYDKVMEYAGKSQVLIFVHSRKETAKTAKAIRDACLEKDTLSAFMREGSASTEILRSEASQVANADLRDLIPYGFAIHHAGMTRVDRTLVEDLFADRHLQVLVSTATLAWGVNLPAHTVIIKGTQIYSPEIGRWTELGALDVMQMLGRAGRPQYDSKGKGIMITHHSELQYYnullSLMNQQLPIESQLISKLADTLNAEIVLGTINNVSDAMNWLGYTYLYVRMVKSPTLYGISHEQTKLDPLLEQRRADLIHTAAnullQLDKASLIKYDRRSGLIQATELGRIASHFYCTHDTMQTYNQLLKATATEIDLFRIFSMSSEFRNIMVREEEKLELQKLAEHVPVPIKESLEESSAKVNVLLQAYISQLKLEGFALQSDMVFISQSAGRLFRALFEIVLWRGWAHLAQAQSSWRLQNDKCSTVAVVRSIDKKNLSFERLYDLDQHQLGELVKMPKMGKPLYKFIRQLPKLDMTALIQPITRSTLRIELTITPDFLWDARVHGTAEGFWIFVEDVDGELILHHEYFLLKQKFCEDEHLVKMFVPVFDPLPPLYFIRIVSDRWLGSETVLPISFRHLILPEKYPPPTELLDLQPLPLSALNSKLFQSVFEQKNISVFNPIQTQERNKTRADFRNTIYAGTNCGFTSLnullIKSGNLIYFLFQFFnullTVYEGNDNVFIGAPHGSGKTVCAEFAILRHFDNRPDAKAVYVTPMEDLAEKKFTDWQDRIGTALEKTVVMLTGEPSTDLKLLQRGQLIIATPEKWDNVSRRWKQRKNVQAVRLFIVDDLHMIGGSNGPVLEVICSRMRYMSSQLDSTVRIVALSSSLANARDGFNLSHTASRLAAMARPVYAAVVRHGGKLRPRPALVFVPSRRQSRSTAVDMLTMAHADGQSKRFLHINPQEPSFIRLLDNVQDKTLKETLSCGVGFLHEGTGVRDMAVVEQLFQSGAIQVCIVPRTMCYQISMSAYVVIIMDTQFYNGKYHVYEDYPIGDVLHMVGLANRPGIDDDAKCVLMCQSSKKDFFKKFLYEPLPVESHLDHCLHDHFNAEIVTKTIENKQDAIDYLTWTLLYRRMTQNPNYYNLQGVTHRHLSDSLSELVENTLKDLENSKCITIKNDMDTQPLNLGMIAAYYYISYTTIEVFSMSLSAKTKLRALIEIISNASEFANMPIRYKEDILLKQLADRLSSQQKHQKFTDPHVKVNLLMNAHLARIQLSAELNKDTEVIILRAIRLVQACVDVLSSNGWLMPAIHAMELSQMLTQAMFTSESYLKQLPHCSTSLLERCKEKKISSIFDLLDLEDDVRQTLLQMTPAEMSDVARFCNHYPSIEVEHKIENNGTITVGDTVNVTVEMERENDLNGMAPPVVAPLFPQKRKEEGWWLVIGDHSSNALFSIKRLTVHQKAKMTLDFTALAVGKMHYKLYFICDSYLGADQEFDLKFRVEETGRSRKRVRDDE

Target ORF information:

RefSeq Version	XM_001900056.1
Organism	Brugia malayi
Definition	Brugia malayi U5 small nuclear ribonucleoprotein 200 kDa helicase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001900056.1

ORF Insert Sequence:

ATGGCCGACG AAATAGCTCG CGTGCAACAG TACGAGTATC GACAGAATAG TAACCTTGTG 
CTGCAAGTCG ATTTCAATTT CACAGATCGT CGTGGTCGCG ATGAACCTAC CGGTGAAGTT 
ATGCCACTCA CTAAGGAGGT TTTGGCTGGT GTTAAAATGG GCGACAAGTA TCAGCGAGCC 
AAAGCACCTG CAATTGAYAC TAAAAAAGCA AGAAAAAAAA AGCGAACTGA TGCAAGTGTC 
ACAGGGAATA GTACGACTTC GCACACTTTG TTGGGAGATT CAAATACGGA ACTRATGGGC 
TTGTACAAGC CAAGAACGCA AGAAACAAGG CAAACCTATG AAGCAATATT GGCTTATATA 
CAAGATGCCA TCGGAGATCA GCCACGCGAT ATTCTGTGTG GTGCAGCTGA TGAAGTCTTA 
GCAGTATTGA AGTCAGACAA AATTCGTGAG AAAGATCGTA AGAAGGAAGT AGAACTTCTT 
CTTGGATTGT TGACGGATGA AAGAATAGCT GTATTGATTA ATCTTGCCAA GAAAATAACA 
GATTTTTCAG TCGATGACGA AAAAGGGAAA TTTGACAATC AAGATGAAAT AGATGAAACA 
ATCGGTGTAA ATGTACAGTT CGATGATTCT GATGAGGAAG CTGAGGGCGA CGAAGGTATG 
GAGGAAGAAG TAAAAGAAGA AGGTTCAGCT GGGGAAGATG AAGGTGGTGT TGAAGCTGTT 
TTTGAAGAAA CACTTAAGGC TCGATATGTT GATGAGGACG ATGAGAATGC GGGTGGTGAA 
CGAAAAGGAA ATCTGCATGC TCGCGATATA GATGCGCATT GGATTCAGCG GTCACTCTCA 
AAATTTTACA AAGATCCAAT TGTGGCACAG CAGAAAGTTA ATGAAGTGCT GCAAGTTTTG 
AAGGAGGCAT CCGATGACCG TGATTGTGAG AATCAGCTGG TATTATTGCT TGGTTTTGAT 
CAGTTTGAAT TTATCAAAGT GCTTCGCCAG CATCGCCAGA TGATTCTGTA CTGTACTCTC 
TTGAAACAGG CACAAGAAGG TAAAGAAAGG GAAAATATCG AGAAAGAAAT GCTGAGCCGG 
CCGGAATTAC ATCATATTCT TGCTGAACTT CACGAGACAG AAAGTGCAGA TACAGTTGAG 
GCCGAACGGG AAAAGCGTGC ACGAGTACAG CAGCAGCGAA GAATAGCTGA AGCTGAAGGG 
GGTAATGATG AGGGAACAGC AGTGGGTAAC TGGTTACAAT CACGTAAACG TTGTCATTTG 
CCAGATGGCT CGTACCGTAA GCAGAAAAAA AGTTATGAAG AAGTTCATGT ACCGGCGCTT 
AAACCAAAGC CATTCGAGGA AAATGAGAAA CTGGTTGCAA TAACGGACTT GCCAAAATAT 
GCACAGCCAG CTTTCGAAGG ATTCAAGACA CTTAATCGAG TYCAGTCGAG ACTATGCGAT 
TCTGCTTTAA AATCTGATGA ACACTTGCTT TTATGTGCAC CTACGGGCGC TGGTAAAACA 
AATGTTGCTT TACTGTGCAT TTTGCGCGAG ATATCAAAAC ATATGAATGC TGATGGGAGC 
ATACGAATAG ATGAATTTAA ATGCATCTAC ATTGCTCCAA TGAAATCATT AGTACAGGAA 
ATGGTCGGAA CTTTCACAAA ACGCTTAGCA CCTTACAAAA TTGCTGTTGG TGAAATGACT 
GGTGATCAGC AGATGAACAA GGAACAATTC ATGCAAACTC AAGTGATTGT TTGCACGCCT 
GAGAAATACG ATATTGTGAC TCGTAAAGGT GGCGAACGTG CATACAGTCA GTTAGTTGCA 
CTGTTAATTA TTGATGAAAT TCATCTTTTG CACGACAACC GCGGACCAGT GCTAGAAGCC 
ATTGTCGTAA GAACTCTACG ACAAATGGAA CAAAACCATG AAGAATGTCG TTTAGTCGGA 
CTTTCAGCAA CTTTACCAAA CTATCAAGAT GTTGGAACAT TCCTGCGAGT GAAGCCGCAA 
AATTTGTTTT TCTTTGATAA CAGCTTTAGA CCTGTTCCGC TTGAACAGCA GTATATCGGT 
ATTACGGAGA AGAAAGCGTT AAAACGATAT CAAGCAATGA ATGAAGTCGT TTATGACAAA 
GTAATGGAGT ATGCGGGAAA GAGCCAGGTT CTAATTTTTG TACACTCACG GAAGGAAACA 
GCGAAAACAG CAAAGGCTAT ACGTGATGCC TGTCTTGAAA AGGATACTTT AAGTGCATTC 
ATGCGAGAGG GTAGTGCAAG TACTGAAATT CTCCGGAGTG AAGCATCGCA GGTGGCAAAT 
GCGGATTTGA GGGACTTGAT TCCTTATGGT TTTGCAATTC ATCATGCAGG TATGACTCGC 
GTTGACCGTA CCCTTGTTGA GGACTTGTTC GCCGATCGCC ACTTGCAGGT ACTTGTTTCA 
ACAGCTACTC TGGCCTGGGG TGTTAACTTA CCAGCTCATA CCGTGATCAT CAAAGGAACA 
CAAATTTACA GTCCAGAAAT TGGACGTTGG ACTGAATTGG GAGCTTTAGA TGTTATGCAG 
ATGCTTGGAC GTGCTGGTCG ACCTCAGTAT GATTCGAAAG GCAAGGGTAT TATGATAACG 
CATCACTCCG AGTTGCAATA CTATYTATCA CTCATGAATC AACAGTTGCC GATCGAAAGT 
CAGTTAATCT CAAAATTAGC TGATACGCTA AACGCCGAAA TAGTATTGGG AACTATCAAC 
AATGTCAGTG ATGCTATGAA TTGGTTGGGC TATACATATT TGTATGTTCG CATGGTTAAG 
TCACCAACAC TGTATGGGAT TTCACATGAA CAAACGAAAC TGGATCCTTT ACTGGAACAG 
CGTCGTGCAG ATCTGATTCA TACAGCTGCA GCRCAATTGG ATAAAGCTAG TCTTATCAAA 
TATGATCGTA GAAGTGGACT TATCCAAGCA ACCGAATTGG GTCGCATTGC TTCTCACTTT 
TATTGCACTC ATGATACGAT GCAAACATAT AATCAGCTGT TGAAGGCAAC AGCAACTGAA 
ATTGACTTGT TTCGTATATT TTCAATGTCA TCAGAATTCC GGAATATCAT GGTCCGTGAA 
GAGGAGAAGC TAGAGTTACA GAAACTGGCT GAACATGTTC CAGTTCCAAT CAAAGAAAGT 
TTAGAAGAGA GCAGTGCGAA AGTTAATGTA CTTCTACAGG CTTACATCTC ACAACTGAAG 
TTGGAAGGGT TCGCGCTTCA AAGTGATATG GTATTTATTA GTCAGTCGGC AGGACGACTG 
TTTCGAGCAC TGTTTGAAAT CGTATTATGG AGAGGTTGGG CTCATCTCGC ACAGGCACAA 
AGTTCTTGGC GTTTGCAAAA TGATAAGTGC TCGACAGTGG CAGTTGTACG AAGCATCGAT 
AAGAAGAACC TTTCATTTGA ACGCTTATAT GATCTTGACC AACATCAGCT GGGCGAGCTG 
GTGAAAATGC CAAAAATGGG GAAGCCCTTG TACAAATTCA TCCGCCAGTT ACCGAAATTA 
GATATGACAG CATTAATCCA ACCGATAACG CGATCGACTT TAAGAATTGA ACTGACAATT 
ACTCCCGATT TTCTGTGGGA TGCTCGTGTA CATGGAACGG CTGAAGGATT TTGGATATTT 
GTTGAAGATG TTGATGGAGA GCTGATTCTA CATCATGAAT ATTTCCTTTT GAAGCAGAAG 
TTTTGCGAAG ATGAACATTT GGTAAAGATG TTCGTACCGG TCTTCGATCC GCTGCCGCCA 
TTATATTTTA TACGCATAGT TTCCGATCGT TGGCTTGGTA GTGAAACTGT TTTGCCGATA 
TCTTTTCGAC ACTTGATTTT GCCCGAGAAA TATCCACCGC CAACCGAATT ACTGGATTTG 
CAACCATTAC CTTTATCAGC CTTGAACAGC AAGCTTTTCC AGTCAGTATT CGAACAAAAA 
AACATCTCAG TATTCAATCC CATTCAAACA CAAGAACGAA ATAAAACTAG AGCGGATTTT 
CGAAATACGA TTTATGCAGG CACAAATTGT GGCTTCACTT CCTTAYTGAT CAAATCAGGA 
AATTTGATTT ATTTTTTGTT TCAGTTTTTC SGAACGGTTT ATGAAGGTAA CGATAATGTA 
TTTATTGGTG CACCACATGG AAGTGGTAAA ACTGTTTGTG CCGAGTTCGC TATTCTTAGA 
CACTTTGATA ATAGGCCTGA CGCGAAAGCT GTTTATGTGA CGCCAATGGA AGATTTAGCT 
GAAAAAAAGT TCACCGACTG GCAGGACCGA ATTGGAACTG CACTAGAAAA AACAGTTGTT 
ATGTTAACGG GTGAGCCATC GACTGATCTG AAGTTACTAC AGCGAGGACA GTTGATCATA 
GCAACACCAG AAAAGTGGGA CAATGTTAGT AGACGTTGGA AACAGCGAAA GAATGTTCAA 
GCTGTGCGGT TATTTATTGT TGACGATCTT CATATGATTG GAGGCAGTAA TGGGCCGGTA 
CTGGAAGTAA TTTGCTCACG CATGCGTTAC ATGTCGTCAC AGCTGGATTC AACAGTTCGA 
ATTGTTGCTC TTTCATCATC ACTAGCTAAT GCCAGAGACG GTTTTAACTT ATCTCACACT 
GCTTCTCGGC TGGCTGCAAT GGCACGACCG GTTTATGCAG CAGTCGTCCG ACATGGTGGC 
AAGTTGCGTC CACGCCCTGC ACTCGTTTTT GTGCCTAGTA GACGGCAGTC GAGATCAACT 
GCTGTCGATA TGCTTACAAT GGCACATGCT GATGGACAGT CGAAGCGGTT TTTGCATATT 
AATCCACAAG AACCAAGTTT TATTCGATTG CTCGATAATG TTCAGGATAA AACTCTAAAA 
GAAACATTAT CTTGTGGAGT AGGTTTTCTA CATGAAGGTA CAGGTGTTAG AGATATGGCC 
GTTGTGGAAC AGCTTTTTCA AAGTGGCGCA ATTCAGGTAT GCATCGTTCC GCGCACGATG 
TGCTATCAAA TTTCCATGAG TGCGTATGTC GTAATCATTA TGGACACACA ATTTTATAAT 
GGAAAGTACC ATGTCTATGA AGATTATCCA ATAGGCGACG TTTTGCACAT GGTTGGACTA 
GCGAATAGAC CCGGGATTGA TGACGATGCA AAATGTGTCC TCATGTGTCA GTCTTCTAAA 
AAGGATTTTT TCAAAAAGTT CCTTTATGAA CCACTTCCTG TTGAGTCACA TTTGGATCAT 
TGTCTTCATG ATCACTTTAA TGCCGAAATT GTGACTAAAA CAATCGAAAA TAAACAGGAC 
GCAATCGACT ACCTTACATG GACTCTGTTA TATCGACGTA TGACACAGAA TCCAAATTAT 
TACAATTTGC AAGGTGTGAC GCATCGTCAT TTATCAGACA GTCTTTCCGA ACTAGTAGAA 
AATACGCTGA AAGATCTGGA AAATTCGAAA TGCATTACAA TCAAGAACGA TATGGACACA 
CAGCCACTTA ATTTAGGAAT GATTGCAGCC TACTACTACA TAAGTTATAC AACAATCGAA 
GTATTCAGCA TGTCTTTGAG TGCGAAAACT AAGCTGCGAG CTCTTATTGA AATTATCAGT 
AATGCAAGTG AATTTGCAAA TATGCCTATA CGTTACAAAG AAGATATTTT GTTGAAACAA 
CTGGCTGACC GATTATCAAG CCAGCAGAAA CACCAGAAAT TTACAGATCC ACATGTTAAG 
GTCAATTTAT TAATGAATGC TCACTTGGCT CGAATACAAT TGTCAGCTGA ACTGAATAAA 
GATACAGAAG TTATCATTCT TAGAGCAATC CGTCTAGTGC AAGCATGTGT TGATGTTTTA 
AGCAGCAATG GTTGGTTAAT GCCTGCAATT CATGCAATGG AACTGTCACA AATGTTAACA 
CAAGCGATGT TCACCAGTGA ATCATATTTG AAACAACTAC CCCATTGCTC AACTTCATTA 
CTTGAACGAT GCAAAGAAAA AAAAATATCA TCAATATTTG ATTTACTGGA TTTGGAAGAT 
GACGTCCGAC AGACACTTTT ACAAATGACT CCAGCAGAAA TGTCGGATGT TGCTCGTTTT 
TGCAATCATT ATCCTAGCAT TGAAGTTGAA CACAAAATTG AAAATAATGG AACCATTACT 
GTGGGTGATA CAGTGAATGT TACCGTAGAA ATGGAACGTG AAAATGATTT GAATGGAATG 
GCTCCACCTG TAGTAGCACC CCTTTTTCCG CAGAAGCGGA AGGAAGAAGG GTGGTGGTTG 
GTTATTGGGG ATCATTCTTC TAATGCATTG TTTTCCATAA AAAGGTTAAC AGTCCATCAG 
AAAGCAAAAA TGACGCTTGA TTTTACTGCA TTGGCAGTAG GGAAGATGCA TTATAAGCTA 
TACTTCATTT GTGATTCATA CCTAGGAGCT GACCAAGAAT TCGATCTCAA ATTTCGTGTC 
GAAGAAACTG GACGTAGTCG AAAGCGAGTT CGTGACGATG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Draft genome of the filarial nematode parasite Brugia malayi.
Science (New York, N.Y.)317(5845)1756-60(2007 Sep)
Ghedin E,Wang S,Spiro D,Caler E,Zhao Q,Crabtree J,Allen JE,Delcher AL,Guiliano DB,Miranda-Saavedra D,Angiuoli SV,Creasy T,Amedeo P,Haas B,El-Sayed NM,Wortman JR,Feldblyum T,Tallon L,Schatz M,Shumway M,Koo H,Salzberg SL,Schobel S,Pertea M,Pop M,White O,Barton GJ,Carlow CK,Crawford MJ,Daub J,Dimmic MW,Estes CF,Foster JM,Ganatra M,Gregory WF,Johnson NM,Jin J,Komuniecki R,Korf I,Kumar S,Laney S,Li BW,Li W,Lindblom TH,Lustigman S,Ma D,Maina CV,Martin DM,McCarter JP,McReynolds L,Mitreva M,Nutman TB,Parkinson J,Peregrín-Alvarez JM,Poole C,Ren Q,Saunders L,Sluder AE,Smith K,Stanke M,Unnasch TR,Ware J,Wei AD,Weil G,Williams DJ,Zhang Y,Williams SA,Fraser-Liggett C,Slatko B,Blaxter ML,Scott AL

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Bm1_43145 cDNA ORF clone, Brugia malayi

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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