BRAFLDRAFT_129259 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_129259
Entrez Gene ID	7252319
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002612172.1	XP_002612218.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_129259 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_129259 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb19803	XM_002612172.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_129259 ( XM_002612172.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002612218.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb19803
Clone ID Related Accession (Same CDS sequence)	XM_002612172.1
Accession Version	XM_002612172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101555). COMPLETENESS: incomplete on the 5' end.

ATGTCAGAAG TCCAGAGAGA GGAGGAACTG AGGGACCCAC CCTATGGGTA TCCGGAAAAG 
CAGGGCCTGT ACGACCCGGC CTATGAGAAG GATTCCTGTG GCGTAGGCTT TGTCGTCAAC 
ATCGAGGGAA ACAGATCGCA CAAGGTGTTG CGAGACTCCC AGACGATGTT GGAGCGTATG 
GAACACCGCG GGGCGTGCGG ATGTGACTCC GATACCGGTG ACGGGGCCGG CTGCCTGACC 
GGTATCCCAC ATGAACTCTA CAGCCGTGTA CTCAAAGAGG AGCAGGGTAT TGAGCTGCCC 
CCACGAGGGA AGTATGCCAC TGGTCTCCTT TTCCTGGGCA AGAACACCTA CCAACAGAGC 
CAGGACATCT TCCAACAGAT TGCTGAAAAG TGTCGTTTGA GTGTGTTGTG TTGGCGTACG 
GTACCAACAG ATGAGACTAA GATTGGCACG GTGGCCAGGG AGACCGAACC ATGCATCAAA 
CAGGTGTTTG TGACAGGTGA CATGGAGGCT GAGGAGTTCC GTCGGCAGGT GTTCTCTCTC 
AGGAAGCAGG CCACGCACAA GATCTCCACC GACAGTCGCC GCTTCTACAT CTGCAGTCTC 
AACCAGGACA TCGTGGTCTA CAAGGGCCAG ACCACCACGA CCCAGTTGTG GCAGTATTTC 
CTGGACCTGC AGGACCCAGA CTACCTGACC CACCTGTCCC TGGTGCACAG TCGCTTCTCT 
ACTAACACCT TCCCTAGCTG GGAGAGGGCA CACCCACAGA GATACCTGGC CCATAACGGT 
GAGATCAACA CCCTGCGCGG GAACGTGAAC CTGATGCGAG CGCGGGAGGG CGTGATGTCC 
AGCCTGTCGT TCAAGGACCA ACTGAAGGAG CTGTATCCCG TGGTCGAGAA GGACCAGTCC 
GACTCGGGAT GTTTCGACAA CGTCCTGGAG TTTCTGGTCA ACGCTGGCGG CAGATCTTTA 
CCCGAGGCTG TGATGACCAT GGTACCTGAG GCCTGGCAGA ATGATAAGTA TATGGAGAAA 
GGCAAGAAAG ATTTCTACCA GTGGAGCGCC TGTGCTATGG AGCCCTGGGA TGGACCAGCA 
CTGTTCACGT TCTCCGACGG GCGGTACATT GGTTCCATCC TGGACCGAAA TGGGCTTCGT 
CCCTCGCGCT ACTACGTGAC CAAGTCGGGC TATCTGGTGA TGGCTAGTGA GGTGGGAGTG 
TTGGACATCC CTCCAGAGGA TGTGCTGAGA AAGGGCCGCC TGAAGCCTGG CAGAATGCTA 
CTTGTTGACA CCCAGGTGGG TGAGATCAGT GGAGACACTG AGCTGAAGAG ACAAATAGCT 
GGTGCCAGAC CTCATGGGGA TTGGTTAAGC AAGCTTGTGA CATTGGATGA CCTCTACAAA 
GCACAACCTA CTGATGAAGT GAACAACAAC ATCACCCGCT ACCCGAATGT GGAGGATGAC 
CGACGACTGC CCCTGTATGG TTACACTATT GAGACCCTCA GTATGCTGGT GCTGCCTATG 
ATCAAGAACA AGAAAGAGGC CCTAGGCTCC ATGGGTAACG ATGCTCCGCT GGCCTGTCTG 
TCTCAGTACA GCCCTCTGGT GTTTGAGTAC TTCAAGCAGA TGTTTGCTCA GGTCACCAAC 
CCGCCCATCG ACCCATTCAG GGAGAGAGTC GTCATGTCTC TGGCCTGTCC CATTGGTCCA 
GAGTCGAACA TCCTGGAGCC GAGCGAGGGC CAGTGCAGAC GACTGTTCCT GCAGCACCCC 
ATGTTGACCC TACAGGACCT GGAGGTCATC AAGGCTACCA GGGTGCATGG CTGGAAGACC 
TGTACCATTG ACGTGACCTA TCCTGTTGAA GACGGTACCC GTGGACTGGT GCCTGCCCTA 
AACCGTGTGT GTGAGGACTG CTCTCAGGCC ACGAAGGAAG GCTTCACTTA CCTAATCCTG 
TCTGACCGAG CCGCATGCAA GGAGAGAGTG CCTATTGCCT CTCTGCTTGT GTGCGGTGCC 
GTCCACCATT ACCTCATCAA CACCAGACAG AGGCTGAAGG TGGGCCTCAT CCTGGAGACT 
GGCGAAGCAA GAGAGGTGCA CCACCTGTGC TTGTTGCTAG GTTACGGCGC GGATGCCATC 
TGTCCGTACC TGGTGTACGA GAGCGTGATC AACCTGCGTG ACCAGCGGCT GCTGGACCCC 
CCGCTGAGCG AGCAGCACAT CATGGCCAAC TACATCCAGG CCTCGCAGAC AGGCATCAGC 
AAGGTCATGG CCAAGATGGG CATCTCCACT CTGCATAGCT ACAAGGGAGC CCAGATTTTC 
GAGGCAGTGG GACTTGGTAA GGAGGTAGTG GACAAGTGTT TCACCGGCAC AGCCTCCCGC 
CTCGGAGGGG TCACTTTTGA GATGCTGGCA CGAGAGAGCC TAAACCGACA CCATGTGGCT 
TTTGCCGACC GAGACTGTGA CAACTATGTC ATCCAGAACC CTGGGTTCTA CCACTGGAGA 
GATGGGGGTG AGAAGCACAT CAATGATCCC CTCAGCATTG CAAACTTGCA GGATGCAGCC 
AAGAACAAGA ACAAGGGTGC CTACGAGAAG TTTAAGGAGT CGACCCTGGA AGCCGTGAGA 
GCCTGCACCC TGCGTGGACA GTTGGACTTG AAGACTCTGG ACGAACCACT GGATCTGTCG 
CTCATAGAGC CAGCATCGGA AATTGTCAAG CGATTTGCAA CAGGTGCCAT GAGTTTCGGT 
AGCATCTCGA TAGAGACCCA CACCACTCTG GCCAAGGCCA TGAACAGGGT CGGAGCCAAG 
AGTAACACAG GGGAGGGAGG GGAGAACCCT GAACGGTACC AGGACCAGCC TGTAGACAAC 
AGTACCAGGT CTGCCATCAA ACAGGTTGCC TCAGGCAGGT TTGGAGTGAC CAGTGACTAC 
CTGGCACACT CCGACGACCT CCAGATCAAG ATGGCCCAGG GAGCCAAGCC CGGAGAGGGC 
GGCGAACTCC CCGGCCACAA GGTGTCCAAA GACATCGCCA AGACGCGGAA ATCCGTCCCG 
GGCGTCGGAC TCATCAGCCC CCCTCCCCAC CACGACATCT ACTCTATCGA GGATCTGGCC 
GAGCTTATCT ACGATCTAAA AGCGGCGAAC CCGCGCGCCC GCATCAGCGT AAAGTTGGTG 
TCAGAAGTGG GAGTGGGAGT CATTGCCGCC GGCGTGGCGA AGGGAAAAGC GGAACACATC 
ACGATTTCCG GTCACGACGG GGGGACCGGA GCGAGCAGCT GGACGGGCGT GAAGCACGCC 
GGGTTGCCAT GGGAACTGGG AATCGCCGAG ACCCACCAAA CGCTGGTTAT GAACGACCTG 
AGGTCAAGGG TGTGCCTACA GACTGATGGA CAGATCAGGA CAGGTCGAGA CGTAGTGATT 
GCAGGACTGC TGGGTGCGGA TGAGTTTGGC TTCTCCACCG CTCCTCTCAT CACCCTGGGA 
TGTACCATGA TGAGGAAGTG TCACCTCAAC ACCTGTCCTG TGGGCGTGGC CACACAGGAC 
CCAGTGCTGA GAGCCAAATT TGCAGGGAAG CCAGAGTATG TGGTCAACTA CCTTTTCTAT 
CTGGCTGAAG AAGTACGCGA CCTGATGGCC AAGATGGGAT TCCGAACCTT TCAGGAGCTT 
ATCGGTCGCA CGGACGTGCT CAAGATGGCG GAGAAACCGA TCAACGAGAA ATCTGCGCTG 
TTGGATTTCG AGCCAATCCT GAAGAACGCG CTAGACATGC GACCTGGTAT CAACATCGTG 
GCAGGCAGTA TCCAGCAGGA CTTCCAACAA GAGAAGAGAC TGGACAACAA ACTTGTTGAG 
GAGGCCAGTG ATGTACTGGA GGGGAAGAAG CCCACAGTGG ACATCAGAAT GAACATCCAG 
AATGAGGCCA GAGCATTCGC CAGCACCTTG AGCTACAACA TCTCCATGAA ATATGGTGAG 
AAGGGTCTCC CAGCAGGCAG TATCAACATC TACCTGAAGG GGTCGGCTGG ACAGAGCTTC 
TGTGCCTTCC TGGCTCCTGG GGTGAGGGTG GAGCTGGAGG GAGATGCCAA TGACTATGTG 
GGGAAGGGCC TGTCAGGAGG TGAGGTGGTG ATCTATCCCA CACGTAGTCT TCCTGAGGAC 
TTTGACTCCA AAGACAACAT CATCGTCGGT AACGTTGTGC TGTACGGAGC CACGTCTGGC 
AAGGCCTTCT TCAGGGGCCA GGCTGCCGAA CGCTTCTGTG TCAGGAACTC TGGGGCAACC 
GCTGTCTGTG AGGGTTGTGG TGACCATGGG TGTGAGTACA TGACTGGAGG GAGGGTCATC 
ATCCTGGGAC TGACCGGCAG GAACTTTGCA GCTGGCATGT CCGGAGGGTT GGCCTACGTA 
CTGGACACTG AGAAGACATT CCAGACCAAG TGTAACCTGG AGCAGGTATC TCTGGACCCA 
GTGGAGGAGG AGGAAGATGT CAGCTTCCTG AAGGACACCA TCAAGCAGTT CCACGAAGCC 
ACGGGTTCTG TCATGGCCCA GAAGGTCCTC CAGAACTGGG CTGAGTACCA ACCTCAGTTT 
GTCAAGATAT TTCCCCACGA GTATCGCAAG GCACTGGAAA AAATGAAAGA GGAAGAGGCC 
ATGCAGGTTG CCGACGAACC TGTGGCAAAC GACGACAACA AGCTGGTGAA CGGTACCAAC 
GGCAATGGGA AACCAGAGAA GGACATCGAG GACACCATCC CGGATGCTGA GATGGACACC 
AAGAATGAGA AGAGGGTGAT GGAAAAGTTG GACAAGGTTC GCGGCTTTGT GAAGTACGAG 
AGGAACACGG ACGCCTACCG TAACGTCAAA CAGAGGCTCA ACGACTGGAA GGAGATCTAT 
AACCACAAGG GTGTCCGCTC TCAGCTCAAG GTGCAAGCTG CCAGATGTAT GGACTGTGGA 
GTTCCCTTCT GCCAGTCCCA CAGTGGCTGT CCATTGGGCA ACATCATCCC CAAATGGAAC 
GACCTCGTCT TCCATGACCA ATGGAAGGAG GCACTGGACA GGCTGCTGCA GACAAACAAC 
TTCCCAGAAT TCACTGGGAG GGTCTGCCCT GCCCCTTGTG AGGGTGCCTG TGTTTTGGGG 
ATCAATGCAA AGCCTGTGAC CATCAAGAAC ATTGAGTGTG CCATCATTGA CCACGCGTTC 
GAGCAGGGAT GGATCGTACC CCACCAGTAC CCGTCCACTG GGAAGAAGGT GGCCGTGGTC 
GGTAGCGGGC CGTCCGGCCT GGCTGCAGCA GCGCAGTTGT GCAAGGCTGG GCATGCTGTG 
ACTGTGTATG AGAGAAATGA TGAGATCGGT GGACTGTTGC GCTACGGCAT CCCTACCATG 
AAACTGAGCA AGGAGGTGGT ACAACGTCGG GTAGACCTGA TGGCTGAAGA GGGTGTGGTC 
TTCGTGAAGA ACACCCAGAT CGGGAAGGAT CTGCCGGCAA AGACACTGTT GGAGGACAAC 
GACGCAGTCC TGCTGTCGTG TGGAGCCACG TGGCCACGTG ACCTACCTAT CCCAGGCCGA 
AAGCTGGAAG GAATCCACTT TGCGATGAGT TTCCTGGAGA AGTGGCAGAA GAAGCAGCAC 
GGAAACACCA GCATCAGCCC CATCATGGCT AAGGATAAGG ACGTGCTGGT CATCGGTGGT 
GGGGACACAG GCGTGGACTG TATCGGAACT GCACTTAGAC AGGGTGCCAA GACTGTCACA 
ACGTTTGAGA TCCTCCCCGA GCCCCCGAAG GAGCGTGCAG ACAACAACCC GTGGCCGCTG 
TGGCCCCGTG TGTTCCGCGT GGACTATGGT CACGAGGAGG TTAGACTGAA GTACGGCAAG 
GATCCCCGGC ACTTTGAGAT CCTCAGCAAG GAGTTCCTGG ATGACGGTAA AGGTAACGTG 
GCGGGGGTGC GGACCGTGAA GATACAGTGG GTCCGTGACG AGTCCGGCAG GTGGCAGATG 
AAGGACGTGC CTGACAGTGA AAGGATCTTC AAAGCTGACC TCATCCTGCT GGCTATGGGC 
TTCCTGGGGC CTGAGAACAC CATCGCTGAT GAGCTGGGAG TGGACCTGGA CCCACGCTCC 
AACTTTGACA CCTCCAGTGC TCCATACAAG TGCAACATTC CCAAGCTGTA TGCCGCTGGA 
GACTGTCGGC GAGGCCAGTC CCTGGTGGTG ACCGCCATCT CAGAGGGTCG CCAGGCGGCC 
AGACAGATCG ACCTGGACCT GATGGGCTTT ACGTCATTGG CCGGCCCGGG CGGTGTCGTC 
ACGGCAACCA AACTCACCAA GAAGGAATCT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002612218.1
CDS	1..6216
Translation	MSEVQREEELRDPPYGYPEKQGLYDPAYEKDSCGVGFVVNIEGNRSHKVLRDSQTMLERMEHRGACGCDSDTGDGAGCLTGIPHELYSRVLKEEQGIELPPRGKYATGLLFLGKNTYQQSQDIFQQIAEKCRLSVLCWRTVPTDETKIGTVARETEPCIKQVFVTGDMEAEEFRRQVFSLRKQATHKISTDSRRFYICSLNQDIVVYKGQTTTTQLWQYFLDLQDPDYLTHLSLVHSRFSTNTFPSWERAHPQRYLAHNGEINTLRGNVNLMRAREGVMSSLSFKDQLKELYPVVEKDQSDSGCFDNVLEFLVNAGGRSLPEAVMTMVPEAWQNDKYMEKGKKDFYQWSACAMEPWDGPALFTFSDGRYIGSILDRNGLRPSRYYVTKSGYLVMASEVGVLDIPPEDVLRKGRLKPGRMLLVDTQVGEISGDTELKRQIAGARPHGDWLSKLVTLDDLYKAQPTDEVNNNITRYPNVEDDRRLPLYGYTIETLSMLVLPMIKNKKEALGSMGNDAPLACLSQYSPLVFEYFKQMFAQVTNPPIDPFRERVVMSLACPIGPESNILEPSEGQCRRLFLQHPMLTLQDLEVIKATRVHGWKTCTIDVTYPVEDGTRGLVPALNRVCEDCSQATKEGFTYLILSDRAACKERVPIASLLVCGAVHHYLINTRQRLKVGLILETGEAREVHHLCLLLGYGADAICPYLVYESVINLRDQRLLDPPLSEQHIMANYIQASQTGISKVMAKMGISTLHSYKGAQIFEAVGLGKEVVDKCFTGTASRLGGVTFEMLARESLNRHHVAFADRDCDNYVIQNPGFYHWRDGGEKHINDPLSIANLQDAAKNKNKGAYEKFKESTLEAVRACTLRGQLDLKTLDEPLDLSLIEPASEIVKRFATGAMSFGSISIETHTTLAKAMNRVGAKSNTGEGGENPERYQDQPVDNSTRSAIKQVASGRFGVTSDYLAHSDDLQIKMAQGAKPGEGGELPGHKVSKDIAKTRKSVPGVGLISPPPHHDIYSIEDLAELIYDLKAANPRARISVKLVSEVGVGVIAAGVAKGKAEHITISGHDGGTGASSWTGVKHAGLPWELGIAETHQTLVMNDLRSRVCLQTDGQIRTGRDVVIAGLLGADEFGFSTAPLITLGCTMMRKCHLNTCPVGVATQDPVLRAKFAGKPEYVVNYLFYLAEEVRDLMAKMGFRTFQELIGRTDVLKMAEKPINEKSALLDFEPILKNALDMRPGINIVAGSIQQDFQQEKRLDNKLVEEASDVLEGKKPTVDIRMNIQNEARAFASTLSYNISMKYGEKGLPAGSINIYLKGSAGQSFCAFLAPGVRVELEGDANDYVGKGLSGGEVVIYPTRSLPEDFDSKDNIIVGNVVLYGATSGKAFFRGQAAERFCVRNSGATAVCEGCGDHGCEYMTGGRVIILGLTGRNFAAGMSGGLAYVLDTEKTFQTKCNLEQVSLDPVEEEEDVSFLKDTIKQFHEATGSVMAQKVLQNWAEYQPQFVKIFPHEYRKALEKMKEEEAMQVADEPVANDDNKLVNGTNGNGKPEKDIEDTIPDAEMDTKNEKRVMEKLDKVRGFVKYERNTDAYRNVKQRLNDWKEIYNHKGVRSQLKVQAARCMDCGVPFCQSHSGCPLGNIIPKWNDLVFHDQWKEALDRLLQTNNFPEFTGRVCPAPCEGACVLGINAKPVTIKNIECAIIDHAFEQGWIVPHQYPSTGKKVAVVGSGPSGLAAAAQLCKAGHAVTVYERNDEIGGLLRYGIPTMKLSKEVVQRRVDLMAEEGVVFVKNTQIGKDLPAKTLLEDNDAVLLSCGATWPRDLPIPGRKLEGIHFAMSFLEKWQKKQHGNTSISPIMAKDKDVLVIGGGDTGVDCIGTALRQGAKTVTTFEILPEPPKERADNNPWPLWPRVFRVDYGHEEVRLKYGKDPRHFEILSKEFLDDGKGNVAGVRTVKIQWVRDESGRWQMKDVPDSERIFKADLILLAMGFLGPENTIADELGVDLDPRSNFDTSSAPYKCNIPKLYAAGDCRRGQSLVVTAISEGRQAARQIDLDLMGFTSLAGPGGVVTATKLTKKESV

Target ORF information:

RefSeq Version	XM_002612172.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002612172.1

ORF Insert Sequence:

ATGTCAGAAG TCCAGAGAGA GGAGGAACTG AGGGACCCAC CCTATGGGTA TCCGGAAAAG 
CAGGGCCTGT ACGACCCGGC CTATGAGAAG GATTCCTGTG GCGTAGGCTT TGTCGTCAAC 
ATCGAGGGAA ACAGATCGCA CAAGGTGTTG CGAGACTCCC AGACGATGTT GGAGCGTATG 
GAACACCGCG GGGCGTGCGG ATGTGACTCC GATACCGGTG ACGGGGCCGG CTGCCTGACC 
GGTATCCCAC ATGAACTCTA CAGCCGTGTA CTCAAAGAGG AGCAGGGTAT TGAGCTGCCC 
CCACGAGGGA AGTATGCCAC TGGTCTCCTT TTCCTGGGCA AGAACACCTA CCAACAGAGC 
CAGGACATCT TCCAACAGAT TGCTGAAAAG TGTCGTTTGA GTGTGTTGTG TTGGCGTACG 
GTACCAACAG ATGAGACTAA GATTGGCACG GTGGCCAGGG AGACCGAACC ATGCATCAAA 
CAGGTGTTTG TGACAGGTGA CATGGAGGCT GAGGAGTTCC GTCGGCAGGT GTTCTCTCTC 
AGGAAGCAGG CCACGCACAA GATCTCCACC GACAGTCGCC GCTTCTACAT CTGCAGTCTC 
AACCAGGACA TCGTGGTCTA CAAGGGCCAG ACCACCACGA CCCAGTTGTG GCAGTATTTC 
CTGGACCTGC AGGACCCAGA CTACCTGACC CACCTGTCCC TGGTGCACAG TCGCTTCTCT 
ACTAACACCT TCCCTAGCTG GGAGAGGGCA CACCCACAGA GATACCTGGC CCATAACGGT 
GAGATCAACA CCCTGCGCGG GAACGTGAAC CTGATGCGAG CGCGGGAGGG CGTGATGTCC 
AGCCTGTCGT TCAAGGACCA ACTGAAGGAG CTGTATCCCG TGGTCGAGAA GGACCAGTCC 
GACTCGGGAT GTTTCGACAA CGTCCTGGAG TTTCTGGTCA ACGCTGGCGG CAGATCTTTA 
CCCGAGGCTG TGATGACCAT GGTACCTGAG GCCTGGCAGA ATGATAAGTA TATGGAGAAA 
GGCAAGAAAG ATTTCTACCA GTGGAGCGCC TGTGCTATGG AGCCCTGGGA TGGACCAGCA 
CTGTTCACGT TCTCCGACGG GCGGTACATT GGTTCCATCC TGGACCGAAA TGGGCTTCGT 
CCCTCGCGCT ACTACGTGAC CAAGTCGGGC TATCTGGTGA TGGCTAGTGA GGTGGGAGTG 
TTGGACATCC CTCCAGAGGA TGTGCTGAGA AAGGGCCGCC TGAAGCCTGG CAGAATGCTA 
CTTGTTGACA CCCAGGTGGG TGAGATCAGT GGAGACACTG AGCTGAAGAG ACAAATAGCT 
GGTGCCAGAC CTCATGGGGA TTGGTTAAGC AAGCTTGTGA CATTGGATGA CCTCTACAAA 
GCACAACCTA CTGATGAAGT GAACAACAAC ATCACCCGCT ACCCGAATGT GGAGGATGAC 
CGACGACTGC CCCTGTATGG TTACACTATT GAGACCCTCA GTATGCTGGT GCTGCCTATG 
ATCAAGAACA AGAAAGAGGC CCTAGGCTCC ATGGGTAACG ATGCTCCGCT GGCCTGTCTG 
TCTCAGTACA GCCCTCTGGT GTTTGAGTAC TTCAAGCAGA TGTTTGCTCA GGTCACCAAC 
CCGCCCATCG ACCCATTCAG GGAGAGAGTC GTCATGTCTC TGGCCTGTCC CATTGGTCCA 
GAGTCGAACA TCCTGGAGCC GAGCGAGGGC CAGTGCAGAC GACTGTTCCT GCAGCACCCC 
ATGTTGACCC TACAGGACCT GGAGGTCATC AAGGCTACCA GGGTGCATGG CTGGAAGACC 
TGTACCATTG ACGTGACCTA TCCTGTTGAA GACGGTACCC GTGGACTGGT GCCTGCCCTA 
AACCGTGTGT GTGAGGACTG CTCTCAGGCC ACGAAGGAAG GCTTCACTTA CCTAATCCTG 
TCTGACCGAG CCGCATGCAA GGAGAGAGTG CCTATTGCCT CTCTGCTTGT GTGCGGTGCC 
GTCCACCATT ACCTCATCAA CACCAGACAG AGGCTGAAGG TGGGCCTCAT CCTGGAGACT 
GGCGAAGCAA GAGAGGTGCA CCACCTGTGC TTGTTGCTAG GTTACGGCGC GGATGCCATC 
TGTCCGTACC TGGTGTACGA GAGCGTGATC AACCTGCGTG ACCAGCGGCT GCTGGACCCC 
CCGCTGAGCG AGCAGCACAT CATGGCCAAC TACATCCAGG CCTCGCAGAC AGGCATCAGC 
AAGGTCATGG CCAAGATGGG CATCTCCACT CTGCATAGCT ACAAGGGAGC CCAGATTTTC 
GAGGCAGTGG GACTTGGTAA GGAGGTAGTG GACAAGTGTT TCACCGGCAC AGCCTCCCGC 
CTCGGAGGGG TCACTTTTGA GATGCTGGCA CGAGAGAGCC TAAACCGACA CCATGTGGCT 
TTTGCCGACC GAGACTGTGA CAACTATGTC ATCCAGAACC CTGGGTTCTA CCACTGGAGA 
GATGGGGGTG AGAAGCACAT CAATGATCCC CTCAGCATTG CAAACTTGCA GGATGCAGCC 
AAGAACAAGA ACAAGGGTGC CTACGAGAAG TTTAAGGAGT CGACCCTGGA AGCCGTGAGA 
GCCTGCACCC TGCGTGGACA GTTGGACTTG AAGACTCTGG ACGAACCACT GGATCTGTCG 
CTCATAGAGC CAGCATCGGA AATTGTCAAG CGATTTGCAA CAGGTGCCAT GAGTTTCGGT 
AGCATCTCGA TAGAGACCCA CACCACTCTG GCCAAGGCCA TGAACAGGGT CGGAGCCAAG 
AGTAACACAG GGGAGGGAGG GGAGAACCCT GAACGGTACC AGGACCAGCC TGTAGACAAC 
AGTACCAGGT CTGCCATCAA ACAGGTTGCC TCAGGCAGGT TTGGAGTGAC CAGTGACTAC 
CTGGCACACT CCGACGACCT CCAGATCAAG ATGGCCCAGG GAGCCAAGCC CGGAGAGGGC 
GGCGAACTCC CCGGCCACAA GGTGTCCAAA GACATCGCCA AGACGCGGAA ATCCGTCCCG 
GGCGTCGGAC TCATCAGCCC CCCTCCCCAC CACGACATCT ACTCTATCGA GGATCTGGCC 
GAGCTTATCT ACGATCTAAA AGCGGCGAAC CCGCGCGCCC GCATCAGCGT AAAGTTGGTG 
TCAGAAGTGG GAGTGGGAGT CATTGCCGCC GGCGTGGCGA AGGGAAAAGC GGAACACATC 
ACGATTTCCG GTCACGACGG GGGGACCGGA GCGAGCAGCT GGACGGGCGT GAAGCACGCC 
GGGTTGCCAT GGGAACTGGG AATCGCCGAG ACCCACCAAA CGCTGGTTAT GAACGACCTG 
AGGTCAAGGG TGTGCCTACA GACTGATGGA CAGATCAGGA CAGGTCGAGA CGTAGTGATT 
GCAGGACTGC TGGGTGCGGA TGAGTTTGGC TTCTCCACCG CTCCTCTCAT CACCCTGGGA 
TGTACCATGA TGAGGAAGTG TCACCTCAAC ACCTGTCCTG TGGGCGTGGC CACACAGGAC 
CCAGTGCTGA GAGCCAAATT TGCAGGGAAG CCAGAGTATG TGGTCAACTA CCTTTTCTAT 
CTGGCTGAAG AAGTACGCGA CCTGATGGCC AAGATGGGAT TCCGAACCTT TCAGGAGCTT 
ATCGGTCGCA CGGACGTGCT CAAGATGGCG GAGAAACCGA TCAACGAGAA ATCTGCGCTG 
TTGGATTTCG AGCCAATCCT GAAGAACGCG CTAGACATGC GACCTGGTAT CAACATCGTG 
GCAGGCAGTA TCCAGCAGGA CTTCCAACAA GAGAAGAGAC TGGACAACAA ACTTGTTGAG 
GAGGCCAGTG ATGTACTGGA GGGGAAGAAG CCCACAGTGG ACATCAGAAT GAACATCCAG 
AATGAGGCCA GAGCATTCGC CAGCACCTTG AGCTACAACA TCTCCATGAA ATATGGTGAG 
AAGGGTCTCC CAGCAGGCAG TATCAACATC TACCTGAAGG GGTCGGCTGG ACAGAGCTTC 
TGTGCCTTCC TGGCTCCTGG GGTGAGGGTG GAGCTGGAGG GAGATGCCAA TGACTATGTG 
GGGAAGGGCC TGTCAGGAGG TGAGGTGGTG ATCTATCCCA CACGTAGTCT TCCTGAGGAC 
TTTGACTCCA AAGACAACAT CATCGTCGGT AACGTTGTGC TGTACGGAGC CACGTCTGGC 
AAGGCCTTCT TCAGGGGCCA GGCTGCCGAA CGCTTCTGTG TCAGGAACTC TGGGGCAACC 
GCTGTCTGTG AGGGTTGTGG TGACCATGGG TGTGAGTACA TGACTGGAGG GAGGGTCATC 
ATCCTGGGAC TGACCGGCAG GAACTTTGCA GCTGGCATGT CCGGAGGGTT GGCCTACGTA 
CTGGACACTG AGAAGACATT CCAGACCAAG TGTAACCTGG AGCAGGTATC TCTGGACCCA 
GTGGAGGAGG AGGAAGATGT CAGCTTCCTG AAGGACACCA TCAAGCAGTT CCACGAAGCC 
ACGGGTTCTG TCATGGCCCA GAAGGTCCTC CAGAACTGGG CTGAGTACCA ACCTCAGTTT 
GTCAAGATAT TTCCCCACGA GTATCGCAAG GCACTGGAAA AAATGAAAGA GGAAGAGGCC 
ATGCAGGTTG CCGACGAACC TGTGGCAAAC GACGACAACA AGCTGGTGAA CGGTACCAAC 
GGCAATGGGA AACCAGAGAA GGACATCGAG GACACCATCC CGGATGCTGA GATGGACACC 
AAGAATGAGA AGAGGGTGAT GGAAAAGTTG GACAAGGTTC GCGGCTTTGT GAAGTACGAG 
AGGAACACGG ACGCCTACCG TAACGTCAAA CAGAGGCTCA ACGACTGGAA GGAGATCTAT 
AACCACAAGG GTGTCCGCTC TCAGCTCAAG GTGCAAGCTG CCAGATGTAT GGACTGTGGA 
GTTCCCTTCT GCCAGTCCCA CAGTGGCTGT CCATTGGGCA ACATCATCCC CAAATGGAAC 
GACCTCGTCT TCCATGACCA ATGGAAGGAG GCACTGGACA GGCTGCTGCA GACAAACAAC 
TTCCCAGAAT TCACTGGGAG GGTCTGCCCT GCCCCTTGTG AGGGTGCCTG TGTTTTGGGG 
ATCAATGCAA AGCCTGTGAC CATCAAGAAC ATTGAGTGTG CCATCATTGA CCACGCGTTC 
GAGCAGGGAT GGATCGTACC CCACCAGTAC CCGTCCACTG GGAAGAAGGT GGCCGTGGTC 
GGTAGCGGGC CGTCCGGCCT GGCTGCAGCA GCGCAGTTGT GCAAGGCTGG GCATGCTGTG 
ACTGTGTATG AGAGAAATGA TGAGATCGGT GGACTGTTGC GCTACGGCAT CCCTACCATG 
AAACTGAGCA AGGAGGTGGT ACAACGTCGG GTAGACCTGA TGGCTGAAGA GGGTGTGGTC 
TTCGTGAAGA ACACCCAGAT CGGGAAGGAT CTGCCGGCAA AGACACTGTT GGAGGACAAC 
GACGCAGTCC TGCTGTCGTG TGGAGCCACG TGGCCACGTG ACCTACCTAT CCCAGGCCGA 
AAGCTGGAAG GAATCCACTT TGCGATGAGT TTCCTGGAGA AGTGGCAGAA GAAGCAGCAC 
GGAAACACCA GCATCAGCCC CATCATGGCT AAGGATAAGG ACGTGCTGGT CATCGGTGGT 
GGGGACACAG GCGTGGACTG TATCGGAACT GCACTTAGAC AGGGTGCCAA GACTGTCACA 
ACGTTTGAGA TCCTCCCCGA GCCCCCGAAG GAGCGTGCAG ACAACAACCC GTGGCCGCTG 
TGGCCCCGTG TGTTCCGCGT GGACTATGGT CACGAGGAGG TTAGACTGAA GTACGGCAAG 
GATCCCCGGC ACTTTGAGAT CCTCAGCAAG GAGTTCCTGG ATGACGGTAA AGGTAACGTG 
GCGGGGGTGC GGACCGTGAA GATACAGTGG GTCCGTGACG AGTCCGGCAG GTGGCAGATG 
AAGGACGTGC CTGACAGTGA AAGGATCTTC AAAGCTGACC TCATCCTGCT GGCTATGGGC 
TTCCTGGGGC CTGAGAACAC CATCGCTGAT GAGCTGGGAG TGGACCTGGA CCCACGCTCC 
AACTTTGACA CCTCCAGTGC TCCATACAAG TGCAACATTC CCAAGCTGTA TGCCGCTGGA 
GACTGTCGGC GAGGCCAGTC CCTGGTGGTG ACCGCCATCT CAGAGGGTCG CCAGGCGGCC 
AGACAGATCG ACCTGGACCT GATGGGCTTT ACGTCATTGG CCGGCCCGGG CGGTGTCGTC 
ACGGCAACCA AACTCACCAA GAAGGAATCT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_129259 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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Target ORF information:

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