BRAFLDRAFT_79472 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_79472
Entrez Gene ID	7218043
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002588471.1	XP_002588517.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_79472 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_79472 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb05753	XM_002588471.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_79472 ( XM_002588471.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002588517.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb05753
Clone ID Related Accession (Same CDS sequence)	XM_002588471.1
Accession Version	XM_002588471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101348). COMPLETENESS: incomplete on both ends.

ATGGCGGACC CACGTGTGGT GCACGGAGAG GGACAAGTTG CCGTCCACAA AGTGAACGAG 
TCGGAATGCT CCCCACCCAC ATGCACCTGC ATTCCTAAAG ACAAGGAATG TTACATCAGG 
AACTACCAAT TTGAACTAGA TGACGACAAG ATGAACAACT CACTTGGAAC CCACGACTTT 
GACTATTACT TTGTCATAAC AGTCACAAAT AATGCCATGT TACAGACACA GACGACATTC 
CAGGTGACAG TTGACGCCAG CCCCCCACTA CCCGGCCATG TTCACGACAG TCTTCCCGGT 
GAGACAGACA CAGACTACCA GCAGGACGTC ATCATACAGG CATCATGGGA AGGGTTCTTT 
GACAGAGAAA GTGGCGTCAT GTTTTACCTG GTCGCTATCA CACAGGAATG CCTTACCCTG 
GAGATCATGG CATCAACTGG CAACGATTCT TTTAGAACGA ATACGACTTC TACTTACGTC 
ACATGGGAGG CACCACATCC GGGCACCTAC TACTGCACTG TTGTGGCCTT CAACCGTGCT 
CTGGAGCCGT CAGAACCCGT ATGTTCAGAC GGTGTGACTG TAGACACCAC CCCGCCCGTG 
CTGAGGGAGG TTGTTGTAGA TAACGCACAC TACAAACCGG GACTTCTTCA GGACGGAGAA 
GGAAATGTGT GGATGGTGGA CAGGAATGGG TACCGATATC CGGCTCCAAC GTGTGGGAAC 
AGGTCAAGAG AGGTAGATGA CATTGAACTG TGGCCACTCT TACAACCTCT AAACAGCTCG 
GTGGATCCTA AGGACATTGA TGACAGCGAC TGCCATGTGT ACGAGCCGCT GTCAACCAAA 
GCATACTTGT CTCAAGATAA TAAACTGACG GTCCATTGGT ACGGTCAAGA CCAGGAAAGT 
GACATCTATG ACTACGAGGT TGGGCTGAGC AGTACTGAGT CACCAGACGT TCCGGATGTA 
CTTCCGTTCA TATCCACAAA CTCCCGTCCG CACTTCAGCA CCTACCATCC CAACCTGGGG 
GAGGGAAAAC AGTTCTACCT GGTGATAAGG GCCATCAACA GGGCGCAGAT GGCTACCACT 
AAGGTCCTCG GCCCATACAT AGTGGATGTT ACACCGCCGA CATTTTCTGG TCAGATAAAA 
GTGAGTCTTG AACAGCAGGA TAAACAGCAG ATGCTGGTGG GACGATGGGA GGAAGACGCG 
TTCTTTGAGA CAGACGGGGA TGATGCTCTT ATTACGTATG AGTTTGCTGT TGGCCACAGC 
CCTGGGAGCT CCGAGGTGCA CCCCTTCACA CCCCTGCCGG CCACCGGTAA TGACCTGTGT 
AACGTTACCT TCCCACCTGT ATGCGTGTCC GTCCCCGTGT CATCTCTGGA CTGGCAGCTC 
CACGGGACGC ACACGTACTA CCTGTCTGTC CGTGTGGAGA ACACGGCTGG GCTCAGCCTG 
GTCGCCTCGT CAGGACCGTA CGTACATGTT ATAGACTTAC CGTCCAGAGG AGTCGTCATG 
GAAGTGTCTG ATGCACAACG GGAGGCTTCT GTGTTTGGGC ACGAGGGAGA CATTGACTTC 
CAGAACACCA CCGAAGAGCT CCACTGTGAG TGGCGAGGGT TCAGCCATCC ACATCTTACC 
GTGACGTATC AGGTTGGTGT GGGCACAACA CCAGGTGCTG ATGACGTCAT TACTTTTGCT 
GATGCAGGAA TTGAGACGCA TTTTTCGTGG ACACATCTAC AGCTCCAGAG GTTTCAGATG 
TATTACGTCA CAGTCATAGC CCTGACTGAA GCAGGAAATA CATCTGTGAC GTCAGACGGA 
GTGCGGGTCG TACTAGATGA TGACGTCATC AACGGTACAA ACGTTTATGA TGGACAAGAA 
TGCAATGCAC CTGGCTATAA CATTACGTTT TCCCACCACG CCGGTTTCCC GTATGGAGAC 
TGCGCTGAGG ACATTAACTA TCAGGCTTCA ACAACAGCCG TCGGCGCTCA TTGGTCGATT 
ACAGACGACA TACGGCCGTT TGTCCGCCAT ATTGAATGGT CTCTTCAGCG AGAAGATGTG 
ATTAATGGTG CCACTGTCTG GGAGCAGGTG ACTGACTTCA GGAATGTACA GCTGTCACCT 
GAAGCCACCT GGACCTCCCT CAGTCTGACG AGTGGAGACA AATACCGGTC TGTGTTGAGA 
CTGTGTCATA CAGCTGGATG TTTCAAGCCG TTGTTCAGTG ACGGATTCCG GGTTCTACAC 
GACCCTCCCA TCCCTGGCCA GATCACAGAC ATCAGCTACA ACAGCACGGC TGGCGTCCTG 
ACGTTCACAT GGGAACGGTT CACACACGGT CACATGACAC CTGGGTCCGA CAGCTACATG 
CTCGGGTACG AATGGACGCT CACCAGTGAG ATGTCATCAC GGCCTGCTAA ACATGGTGAC 
GTCATTCTGC CATGGCAAGC CATTGGGCAC CCCACACAGA CAGGCAGTAA GCTTACCTGC 
ACGGTGACGT TTGACCATCC CCTGGACTTC ACCCACTGCC TGCGTCTGCT GGTACGAGGC 
TACAACAAGG CGGGGCTGTC CTCCAGCTCT AGCCGGGATG TCATCAACTG TGACACCGAC 
AACCCGGCAG ACTTCAGCAC GCCGATTGTC ATCGATGCAG TGGGCGAGTA CGACCAACTG 
TCTTCCAACA AGACAAGTGA CATATTCTTA GAGACCAACC AAAGATGGTC GTCGCCTGAC 
AAAGAGTTCA CCCCAGCTTC TACCAAACTG TCAGCTGTCT GGCCGACATT ACGTCATGGT 
CTGTACAACT GGAAGGTCAT CTCTGCAGAC TCCATACGTC ACTGGGCTTA CGTCACTTCC 
CAGCATGCTC TGCGCTACTC GGACTACGAG TGTGACAGTC CAGAGGTGCT GGCCTGTGGC 
ACATCAAAGG ATAATTTCGT GAACGTACCT GGTCTCCAAC TCCACCACGG ACGGCGGTAC 
CACATCTGTA TCCACGCCGA CAGGACAGTC CTGCAGTTTG AGACGTTTGA GCAGACCCTG 
GCTGAAGTAG ACACCTGTTC TAACGGTATC ACTGTGGACC TGACACCGCC CTTACCTGGA 
CAGGTGTGGA TCAACAGGAG GGACCGGGAC TTTCAGACGT CAGTATCCGA ACTGTACGTT 
TTCTGGGACT CGTTTGTTGA CGTGGAGGAG CACGGGATGT CATCTCACCA CAGCGGCATA 
CAGAAATATG AACTGGCAAT AGGGTCTTCG CCTAGTGGTG TAGACATCCA GGACTTCAGA 
GATCAGGGTG TAACTAACAT GGCCGCCGTG CACAACCTTC ATCTACAGCA GGGGCACACG 
TATTACGCCA CAATCAGGGC AACCGACTAC GTTGGATTCT CTGTGACAAG TGTATCACAA 
GGTGTGACCA TTGACGTCAC CGCTCCTATG GTAACGGATG CCAAGATTGA CGTCGGTGGC 
CGTTATCATC TGTCTACGTC ATCAGTGACG GCGAGATGGG ACGGCGTTTT CCTGGACCCC 
GAGAGTGGTA TATCTCACTA CGAGTGGAGT GTGGGGTCCG GCCCGGGTCA TGCTGACATC 
ATGCCGTTCA CACGGACGGA GTCTGAAGAA GCCGTGTCAG ACCCAGCACT GGATCTACAG 
CTGCAAGAGG GACATGCCTA CTATGTGTCT GTTAAGGCGT ATAACGGAGC AGGACTGTCA 
GTGATCGCGG TATCATGGCC GACTGTAGTG GAGAGCTCCC CGCCTGAACC TGGGGTTGTC 
TATGACGGGG TTCCATCAGC GCATCACCAG GACGCAGACT TTCAGACGGA GCTGGACACG 
CTGCGTGCCA GATGGGAGGG TTTCTATGAC GCTCACACCG CCATTTTGGG GTACGAATGG 
AATGTGGGGA CATGTCCCGG ATGTGATGAC GTCATGGAAG CGGAACATGT CGGCTTGCTT 
ACCGATGTTT CTGCGAGTCA TCTCGGCCTT GTCCCCGGTA AGAAGTATTA CGTCACAGTA 
ACCGCCTGTA ACGCAGCAGA CCTCTGTACG TCAGTCTCAT CTGATGGCGT CATCATCGAC 
AACTCCCCTC CGGCCCCGGG GGTGGTGTAT GATGGGGTGG ATGACATGGA CAGTCACTTC 
CAAGCATCAA GAACAACTCT GGGAGCACAT TGGTTTGGCT TCCACGATCC ACACTCCGGA 
TTGTCTCACT ACGAGTGGCG TGCAGGCACG ACCCCTGGCG GAGAAGACTT GTACGGCACC 
ACGCGACTAC ATCTGACAGA GTTGGTCCAC ATCTCCCAGC TGGATCCTCC TCTTCCACTC 
AACCATACCG TGTACATTAC AGTTAGAGCA TACAACCAAG CAGGATTGTA CGTTGAGAGA 
ACGTCCAACG GCTTCTATGT TGACGAATCT TCACCAGTGA TGTTAGAGGA ACCACAGTTT 
GATCTGACCC AGGGGTCCAT TATTCCTGAT ACACAGGTGT GGAGAACAAC CCTGTCCGTG 
AGGTGGGAGG TTGTTGACCC CGAGAGTTCC ATCGAGCGGC AGCACCTGTC AGTCATCACT 
CACCACCAGG CCGGGATGGA CATAGAGCCT GTGGAGATCG CCGGTTCCAT CCGAGACTTC 
AGGTTTACTA ACCTATCACT ACATGACGGG GACACGTACT ACGTACGGCT GGTGGCCTGT 
AACGGTGCCA AGCTGTGTAC ATCAGGGCAG ACTCACGGAG TTCTGGTGGA CTCCACCCCT 
CCCACAGTAG GTATGTTAGC CGTACAGACC GACCATGCTG CCCAGTTATG GCGTCACCGT 
GACGGCTGGA TGACGTACCA CCAACAGAAC GGCACCGCCC CGCCTCACGT CAGGTTGGCC 
TGGCTCGGCT TCACGGACAT CCACACTGGG ATCCAGGAAT ATCACGTCAC CATCGGGTCG 
ACTTTTGGTG GCACAGATCT CACGGCAAGT TTTCTCAACA TTAGATATGA CGGAGACAGT 
GTGGTCCTCC CGCACCAGGA CGGCCCGTCC CATGAAGACG AAGGTGCCGT ACAAGTCGCT 
GTAGTGAACC TCACACGGAA CCTGACAGAT AGGGAACACA TCTATGTCAC ACTTTGGGCG 
GTGAACAAGG TTGGATTGCA CAGCATTGTG GTCCATGAAG CCCTGGAAGT GGTTCCGTCT 
AACCCGCACA GCGGCCTCCT GTCATTGGTC AGACGATGTG ACGTACATAC TTGTGAGGGC 
GACTGCACAT GCGCACCACA GAACCAGAAA TGTGCTCCAC TTCTCACCGG ATGTCAAAAC 
GTCGCAAACC GTACAGAGTA TCAGCAGGTT CTTGTGTACG ACCTGACGGA CTTGAGTAGT 
CAAACAGCAC CCGACGCTGA CGTCACACTG TCGCAATGCA CTCTGGGCGC AAGATGGGAA 
ACGGTACCGG GAGGTATACC TGTTCAAAGG TGTGAGTGGA GTGCCGGACA GGAGGGAAAG 
GCAGCAGGTA CCGGGGTGTT TGATTTAAAT AACGACAGGA TCTGGTACGA CGTGGGACTG 
AGAACTTCAG CCGTGCTGAG CATCAGGCGG GACAGACGAG AGGACATTGT TCCCTACACC 
AGTTATAACC TGTCAGACAC CACGAGTGAA GTTCTGATAG ACGGTGTGAC GCTACAGTCC 
GGTGTGAAGT ACTTCAGTAA CGTCCGAGCC TACAGCCATG CCGGCTTACA CACTACAGTC 
AGCTCTGACG GCTTCCTGGT GGATACTGAA CCTCCGTCAG CAGGAATAGT TTACGACGGT 
GCGAACATTC ACGACATGGA CTTTCAGAAC TGCAGTTCTC ACGTGGCCGC TAGCTGGCAC 
GGCTTCTCCG ATCTCCAGTC GTACATCCAC CACTATGTGT GGTGTGTGGG ATCCACGCCT 
GGGACTGACG ATATCCTGAC TTGTACAGAT GTGGGAATAA GTCTGGCGGA AGTGAAAGAG 
ATTTCACCTG CACTTCCAAC TGGTACAAAG TACTTCTCTA CGGTGTACGC TGTGGATGCT 
GCAGGACTGA CGTCATCACC CGCAGTGTCT GATGGGATAC TGGTGGACAC CACTCCACCG 
GAAGTGTTGA ACAAGTTTAA TTTCGGTCCA AACCTGCTGG ATAACCCGTC ATTTGAGAAC 
ATTCAGAGTT CTGCAAGCAC CAACCTTATA TCAGACCTTG GAAGTGCTGG TAACGTCGGC 
CTTACAGGAA CAGACAACAG CAGTGCTAGT AACAGCAGTC ACGTCAATTT ACGTAACAAC 
AGCTTTACAT CAGCTAACAA CGGTGAGCCA GCCCACTGGA ACATCTCCGG TGTAGCGGCC 
GTGATGTCAC CGTCCACGGC TGTGTCTCAC CACGGAGTTT CCTACCTCCT CCTGCACGGG 
TCCATCTCAC AACTCATCCC GACCAGTCCT GGTGAGAGAT ACCGCCTGAC GCTTCACGCC 
TCTCCCATCT TCCCCTCTAA CACACCAGTG GTGGCACAGG AGGGGTACGT CATCAGTCCC 
GGCATGCACC GCGTGTTCAA ACTGTACCAG CGGCCGTCCC ACCTGGAGGG GGCGGAGCTA 
CACGTGGACA GGAACATCAC GTGGCACAAG CACGTGTTCT ACTTCACAGC TGTAGAACAC 
GTGTCTGAGG TAGTCATCGG ATCTGTAGGG CCTTGGAGCG GGATTACCCT GGACGATGTA 
AAGGTCCAAG AGATGCGGCA GGGATCCCGC CCCCCTCCAG CCAACCCCAA CCCGACCAAC 
CAGCTGACCA GTCCTGTCCA CCTGGAGACC AGCTTCGCGC ACGACTGGAC CTCCGTGTGC 
GCCGCCTGGG ACTTTGTGGA CCCTGAGAGT CCGATAGTCG GCTATAGCTG GGCCATCGGT 
ACAGTCAGGG GTGGGACTCA GCTTCAAGAC TTCAAGACAG TCGGGAGAAA CACGCATGCG 
CACAATGAAG ACGTTAGGCT GAGTCATGGA TCGTTTGTTC ACGTGACAGT CGTGGCTGAA 
AACGAGGCCG CTCTTCGTTC TGTTGTGTAC TCGGACCCGG TCCTGGTAGA CCTGACACCT 
CCTGTCATAT CTCTGGTCAA GGCAACACTC TACAGCGCCA CCTTTGACCT TACCGGTCAC 
CTGACCCACG GTGATACTGT CTACTCCACT GTCCGCTGTC ATAACTACGC AGGGATGACG 
TCACACGTGA CGTCAAATGG TGTCACCATT GTCAGTCAGC CGCCAAACAG TGATCAAGCC 
ATAGTGGAAA CTATGAGTGC GCCTCAGTTC TACTACCCGA CCCGAGCCTC CCATCAGTCT 
GCTGCTGACG TCATCCACCT GTCCTGGGAA GGGTTCTTTG ATGTTTCCGG CATCAAGAAT 
TATCAGAGAA TTAATAAGGA CATGTCTTGT ATATGTTTGT TCCTGCATCT GGCGTTCTTA 
TTTACAGCAG GCCGGTTCCA GAGCTGGTGG CCCCGGCAGG GAGTGATGAT GTTCGACTGG 
ACAGGCGTGT TCCTCAGTAA CTCCTCCCTC ATTTACGAGG TGTCCATAGG GACCATGGCA 
GCCGGAAGTG ACGTCATGCA GTGGAGAGAA ACAGACAAGA CCGAGATGCG TCTGGACGTC 
AAAAAGTCAC GTCCCTGTTG TCAACAGGAC GGTAGTGATG AAGAAGACGT GGACTTCCAA 
CAGTCCAAGA CCGTCACTGT CCACTGGAAC GTCACTGATG ATGAGAGTGG GGTAGAGTTC 
TGTGAAGTTG CTATAGGTTT GAGTCCAGGA TCAGGAGAAG TCCACCGGTT TACCCAGCAG 
GCAACACTCT ACAGCGCCAC CTTTGACCTT ACCGGTCACC TGACCCACGG TGATACTGTC 
TACTCCACTG TCCGCTGTCA TAACTACGCA GGGATGACGT CACACGTGAC GTCAAATGGT 
GTCACCATTG TCAGTCAGCC GCCAAACAGT GATCAAGCCA TAGTGGAAAC TATGAGTGCG 
CCTCAGTTCT ACTACCCGAC CCGAGCCTCC CATCAGTCTG CTGCTGACGT CATCCACCTG 
TCCTGGGAAG GGTTCTTTGA TGTTTCCGGC ATCAAGAATT ATCAGTGCCG TGTGACTGGC 
CCTGGTTTGA CTGACTTCCC GTGGATAGAT GTTGGTCTGA CCGGACAGAC TCACGCAACC 
CTGTCAGGGC TCCACCTGCA CAACTACAAC AGGTATGAGG TACACGTCCG AGCTGTCAAT 
CACGTGGGCT TGGTCAGCGA TGACGTCATC TCAGAAATCT ATGTGGAGAT GGAGCGACCA 
CTGCAGGCGG GTTCCAGAGC TGGTGGCCCC AGCAGGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002588517.1
CDS	1..8202
Translation	MADPRVVHGEGQVAVHKVNESECSPPTCTCIPKDKECYIRNYQFELDDDKMNNSLGTHDFDYYFVITVTNNAMLQTQTTFQVTVDASPPLPGHVHDSLPGETDTDYQQDVIIQASWEGFFDRESGVMFYLVAITQECLTLEIMASTGNDSFRTNTTSTYVTWEAPHPGTYYCTVVAFNRALEPSEPVCSDGVTVDTTPPVLREVVVDNAHYKPGLLQDGEGNVWMVDRNGYRYPAPTCGNRSREVDDIELWPLLQPLNSSVDPKDIDDSDCHVYEPLSTKAYLSQDNKLTVHWYGQDQESDIYDYEVGLSSTESPDVPDVLPFISTNSRPHFSTYHPNLGEGKQFYLVIRAINRAQMATTKVLGPYIVDVTPPTFSGQIKVSLEQQDKQQMLVGRWEEDAFFETDGDDALITYEFAVGHSPGSSEVHPFTPLPATGNDLCNVTFPPVCVSVPVSSLDWQLHGTHTYYLSVRVENTAGLSLVASSGPYVHVIDLPSRGVVMEVSDAQREASVFGHEGDIDFQNTTEELHCEWRGFSHPHLTVTYQVGVGTTPGADDVITFADAGIETHFSWTHLQLQRFQMYYVTVIALTEAGNTSVTSDGVRVVLDDDVINGTNVYDGQECNAPGYNITFSHHAGFPYGDCAEDINYQASTTAVGAHWSITDDIRPFVRHIEWSLQREDVINGATVWEQVTDFRNVQLSPEATWTSLSLTSGDKYRSVLRLCHTAGCFKPLFSDGFRVLHDPPIPGQITDISYNSTAGVLTFTWERFTHGHMTPGSDSYMLGYEWTLTSEMSSRPAKHGDVILPWQAIGHPTQTGSKLTCTVTFDHPLDFTHCLRLLVRGYNKAGLSSSSSRDVINCDTDNPADFSTPIVIDAVGEYDQLSSNKTSDIFLETNQRWSSPDKEFTPASTKLSAVWPTLRHGLYNWKVISADSIRHWAYVTSQHALRYSDYECDSPEVLACGTSKDNFVNVPGLQLHHGRRYHICIHADRTVLQFETFEQTLAEVDTCSNGITVDLTPPLPGQVWINRRDRDFQTSVSELYVFWDSFVDVEEHGMSSHHSGIQKYELAIGSSPSGVDIQDFRDQGVTNMAAVHNLHLQQGHTYYATIRATDYVGFSVTSVSQGVTIDVTAPMVTDAKIDVGGRYHLSTSSVTARWDGVFLDPESGISHYEWSVGSGPGHADIMPFTRTESEEAVSDPALDLQLQEGHAYYVSVKAYNGAGLSVIAVSWPTVVESSPPEPGVVYDGVPSAHHQDADFQTELDTLRARWEGFYDAHTAILGYEWNVGTCPGCDDVMEAEHVGLLTDVSASHLGLVPGKKYYVTVTACNAADLCTSVSSDGVIIDNSPPAPGVVYDGVDDMDSHFQASRTTLGAHWFGFHDPHSGLSHYEWRAGTTPGGEDLYGTTRLHLTELVHISQLDPPLPLNHTVYITVRAYNQAGLYVERTSNGFYVDESSPVMLEEPQFDLTQGSIIPDTQVWRTTLSVRWEVVDPESSIERQHLSVITHHQAGMDIEPVEIAGSIRDFRFTNLSLHDGDTYYVRLVACNGAKLCTSGQTHGVLVDSTPPTVGMLAVQTDHAAQLWRHRDGWMTYHQQNGTAPPHVRLAWLGFTDIHTGIQEYHVTIGSTFGGTDLTASFLNIRYDGDSVVLPHQDGPSHEDEGAVQVAVVNLTRNLTDREHIYVTLWAVNKVGLHSIVVHEALEVVPSNPHSGLLSLVRRCDVHTCEGDCTCAPQNQKCAPLLTGCQNVANRTEYQQVLVYDLTDLSSQTAPDADVTLSQCTLGARWETVPGGIPVQRCEWSAGQEGKAAGTGVFDLNNDRIWYDVGLRTSAVLSIRRDRREDIVPYTSYNLSDTTSEVLIDGVTLQSGVKYFSNVRAYSHAGLHTTVSSDGFLVDTEPPSAGIVYDGANIHDMDFQNCSSHVAASWHGFSDLQSYIHHYVWCVGSTPGTDDILTCTDVGISLAEVKEISPALPTGTKYFSTVYAVDAAGLTSSPAVSDGILVDTTPPEVLNKFNFGPNLLDNPSFENIQSSASTNLISDLGSAGNVGLTGTDNSSASNSSHVNLRNNSFTSANNGEPAHWNISGVAAVMSPSTAVSHHGVSYLLLHGSISQLIPTSPGERYRLTLHASPIFPSNTPVVAQEGYVISPGMHRVFKLYQRPSHLEGAELHVDRNITWHKHVFYFTAVEHVSEVVIGSVGPWSGITLDDVKVQEMRQGSRPPPANPNPTNQLTSPVHLETSFAHDWTSVCAAWDFVDPESPIVGYSWAIGTVRGGTQLQDFKTVGRNTHAHNEDVRLSHGSFVHVTVVAENEAALRSVVYSDPVLVDLTPPVISLVKATLYSATFDLTGHLTHGDTVYSTVRCHNYAGMTSHVTSNGVTIVSQPPNSDQAIVETMSAPQFYYPTRASHQSAADVIHLSWEGFFDVSGIKNYQRINKDMSCICLFLHLAFLFTAGRFQSWWPRQGVMMFDWTGVFLSNSSLIYEVSIGTMAAGSDVMQWRETDKTEMRLDVKKSRPCCQQDGSDEEDVDFQQSKTVTVHWNVTDDESGVEFCEVAIGLSPGSGEVHRFTQQATLYSATFDLTGHLTHGDTVYSTVRCHNYAGMTSHVTSNGVTIVSQPPNSDQAIVETMSAPQFYYPTRASHQSAADVIHLSWEGFFDVSGIKNYQCRVTGPGLTDFPWIDVGLTGQTHATLSGLHLHNYNRYEVHVRAVNHVGLVSDDVISEIYVEMERPLQAGSRAGGPSRE

Target ORF information:

RefSeq Version	XM_002588471.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002588471.1

ORF Insert Sequence:

ATGGCGGACC CACGTGTGGT GCACGGAGAG GGACAAGTTG CCGTCCACAA AGTGAACGAG 
TCGGAATGCT CCCCACCCAC ATGCACCTGC ATTCCTAAAG ACAAGGAATG TTACATCAGG 
AACTACCAAT TTGAACTAGA TGACGACAAG ATGAACAACT CACTTGGAAC CCACGACTTT 
GACTATTACT TTGTCATAAC AGTCACAAAT AATGCCATGT TACAGACACA GACGACATTC 
CAGGTGACAG TTGACGCCAG CCCCCCACTA CCCGGCCATG TTCACGACAG TCTTCCCGGT 
GAGACAGACA CAGACTACCA GCAGGACGTC ATCATACAGG CATCATGGGA AGGGTTCTTT 
GACAGAGAAA GTGGCGTCAT GTTTTACCTG GTCGCTATCA CACAGGAATG CCTTACCCTG 
GAGATCATGG CATCAACTGG CAACGATTCT TTTAGAACGA ATACGACTTC TACTTACGTC 
ACATGGGAGG CACCACATCC GGGCACCTAC TACTGCACTG TTGTGGCCTT CAACCGTGCT 
CTGGAGCCGT CAGAACCCGT ATGTTCAGAC GGTGTGACTG TAGACACCAC CCCGCCCGTG 
CTGAGGGAGG TTGTTGTAGA TAACGCACAC TACAAACCGG GACTTCTTCA GGACGGAGAA 
GGAAATGTGT GGATGGTGGA CAGGAATGGG TACCGATATC CGGCTCCAAC GTGTGGGAAC 
AGGTCAAGAG AGGTAGATGA CATTGAACTG TGGCCACTCT TACAACCTCT AAACAGCTCG 
GTGGATCCTA AGGACATTGA TGACAGCGAC TGCCATGTGT ACGAGCCGCT GTCAACCAAA 
GCATACTTGT CTCAAGATAA TAAACTGACG GTCCATTGGT ACGGTCAAGA CCAGGAAAGT 
GACATCTATG ACTACGAGGT TGGGCTGAGC AGTACTGAGT CACCAGACGT TCCGGATGTA 
CTTCCGTTCA TATCCACAAA CTCCCGTCCG CACTTCAGCA CCTACCATCC CAACCTGGGG 
GAGGGAAAAC AGTTCTACCT GGTGATAAGG GCCATCAACA GGGCGCAGAT GGCTACCACT 
AAGGTCCTCG GCCCATACAT AGTGGATGTT ACACCGCCGA CATTTTCTGG TCAGATAAAA 
GTGAGTCTTG AACAGCAGGA TAAACAGCAG ATGCTGGTGG GACGATGGGA GGAAGACGCG 
TTCTTTGAGA CAGACGGGGA TGATGCTCTT ATTACGTATG AGTTTGCTGT TGGCCACAGC 
CCTGGGAGCT CCGAGGTGCA CCCCTTCACA CCCCTGCCGG CCACCGGTAA TGACCTGTGT 
AACGTTACCT TCCCACCTGT ATGCGTGTCC GTCCCCGTGT CATCTCTGGA CTGGCAGCTC 
CACGGGACGC ACACGTACTA CCTGTCTGTC CGTGTGGAGA ACACGGCTGG GCTCAGCCTG 
GTCGCCTCGT CAGGACCGTA CGTACATGTT ATAGACTTAC CGTCCAGAGG AGTCGTCATG 
GAAGTGTCTG ATGCACAACG GGAGGCTTCT GTGTTTGGGC ACGAGGGAGA CATTGACTTC 
CAGAACACCA CCGAAGAGCT CCACTGTGAG TGGCGAGGGT TCAGCCATCC ACATCTTACC 
GTGACGTATC AGGTTGGTGT GGGCACAACA CCAGGTGCTG ATGACGTCAT TACTTTTGCT 
GATGCAGGAA TTGAGACGCA TTTTTCGTGG ACACATCTAC AGCTCCAGAG GTTTCAGATG 
TATTACGTCA CAGTCATAGC CCTGACTGAA GCAGGAAATA CATCTGTGAC GTCAGACGGA 
GTGCGGGTCG TACTAGATGA TGACGTCATC AACGGTACAA ACGTTTATGA TGGACAAGAA 
TGCAATGCAC CTGGCTATAA CATTACGTTT TCCCACCACG CCGGTTTCCC GTATGGAGAC 
TGCGCTGAGG ACATTAACTA TCAGGCTTCA ACAACAGCCG TCGGCGCTCA TTGGTCGATT 
ACAGACGACA TACGGCCGTT TGTCCGCCAT ATTGAATGGT CTCTTCAGCG AGAAGATGTG 
ATTAATGGTG CCACTGTCTG GGAGCAGGTG ACTGACTTCA GGAATGTACA GCTGTCACCT 
GAAGCCACCT GGACCTCCCT CAGTCTGACG AGTGGAGACA AATACCGGTC TGTGTTGAGA 
CTGTGTCATA CAGCTGGATG TTTCAAGCCG TTGTTCAGTG ACGGATTCCG GGTTCTACAC 
GACCCTCCCA TCCCTGGCCA GATCACAGAC ATCAGCTACA ACAGCACGGC TGGCGTCCTG 
ACGTTCACAT GGGAACGGTT CACACACGGT CACATGACAC CTGGGTCCGA CAGCTACATG 
CTCGGGTACG AATGGACGCT CACCAGTGAG ATGTCATCAC GGCCTGCTAA ACATGGTGAC 
GTCATTCTGC CATGGCAAGC CATTGGGCAC CCCACACAGA CAGGCAGTAA GCTTACCTGC 
ACGGTGACGT TTGACCATCC CCTGGACTTC ACCCACTGCC TGCGTCTGCT GGTACGAGGC 
TACAACAAGG CGGGGCTGTC CTCCAGCTCT AGCCGGGATG TCATCAACTG TGACACCGAC 
AACCCGGCAG ACTTCAGCAC GCCGATTGTC ATCGATGCAG TGGGCGAGTA CGACCAACTG 
TCTTCCAACA AGACAAGTGA CATATTCTTA GAGACCAACC AAAGATGGTC GTCGCCTGAC 
AAAGAGTTCA CCCCAGCTTC TACCAAACTG TCAGCTGTCT GGCCGACATT ACGTCATGGT 
CTGTACAACT GGAAGGTCAT CTCTGCAGAC TCCATACGTC ACTGGGCTTA CGTCACTTCC 
CAGCATGCTC TGCGCTACTC GGACTACGAG TGTGACAGTC CAGAGGTGCT GGCCTGTGGC 
ACATCAAAGG ATAATTTCGT GAACGTACCT GGTCTCCAAC TCCACCACGG ACGGCGGTAC 
CACATCTGTA TCCACGCCGA CAGGACAGTC CTGCAGTTTG AGACGTTTGA GCAGACCCTG 
GCTGAAGTAG ACACCTGTTC TAACGGTATC ACTGTGGACC TGACACCGCC CTTACCTGGA 
CAGGTGTGGA TCAACAGGAG GGACCGGGAC TTTCAGACGT CAGTATCCGA ACTGTACGTT 
TTCTGGGACT CGTTTGTTGA CGTGGAGGAG CACGGGATGT CATCTCACCA CAGCGGCATA 
CAGAAATATG AACTGGCAAT AGGGTCTTCG CCTAGTGGTG TAGACATCCA GGACTTCAGA 
GATCAGGGTG TAACTAACAT GGCCGCCGTG CACAACCTTC ATCTACAGCA GGGGCACACG 
TATTACGCCA CAATCAGGGC AACCGACTAC GTTGGATTCT CTGTGACAAG TGTATCACAA 
GGTGTGACCA TTGACGTCAC CGCTCCTATG GTAACGGATG CCAAGATTGA CGTCGGTGGC 
CGTTATCATC TGTCTACGTC ATCAGTGACG GCGAGATGGG ACGGCGTTTT CCTGGACCCC 
GAGAGTGGTA TATCTCACTA CGAGTGGAGT GTGGGGTCCG GCCCGGGTCA TGCTGACATC 
ATGCCGTTCA CACGGACGGA GTCTGAAGAA GCCGTGTCAG ACCCAGCACT GGATCTACAG 
CTGCAAGAGG GACATGCCTA CTATGTGTCT GTTAAGGCGT ATAACGGAGC AGGACTGTCA 
GTGATCGCGG TATCATGGCC GACTGTAGTG GAGAGCTCCC CGCCTGAACC TGGGGTTGTC 
TATGACGGGG TTCCATCAGC GCATCACCAG GACGCAGACT TTCAGACGGA GCTGGACACG 
CTGCGTGCCA GATGGGAGGG TTTCTATGAC GCTCACACCG CCATTTTGGG GTACGAATGG 
AATGTGGGGA CATGTCCCGG ATGTGATGAC GTCATGGAAG CGGAACATGT CGGCTTGCTT 
ACCGATGTTT CTGCGAGTCA TCTCGGCCTT GTCCCCGGTA AGAAGTATTA CGTCACAGTA 
ACCGCCTGTA ACGCAGCAGA CCTCTGTACG TCAGTCTCAT CTGATGGCGT CATCATCGAC 
AACTCCCCTC CGGCCCCGGG GGTGGTGTAT GATGGGGTGG ATGACATGGA CAGTCACTTC 
CAAGCATCAA GAACAACTCT GGGAGCACAT TGGTTTGGCT TCCACGATCC ACACTCCGGA 
TTGTCTCACT ACGAGTGGCG TGCAGGCACG ACCCCTGGCG GAGAAGACTT GTACGGCACC 
ACGCGACTAC ATCTGACAGA GTTGGTCCAC ATCTCCCAGC TGGATCCTCC TCTTCCACTC 
AACCATACCG TGTACATTAC AGTTAGAGCA TACAACCAAG CAGGATTGTA CGTTGAGAGA 
ACGTCCAACG GCTTCTATGT TGACGAATCT TCACCAGTGA TGTTAGAGGA ACCACAGTTT 
GATCTGACCC AGGGGTCCAT TATTCCTGAT ACACAGGTGT GGAGAACAAC CCTGTCCGTG 
AGGTGGGAGG TTGTTGACCC CGAGAGTTCC ATCGAGCGGC AGCACCTGTC AGTCATCACT 
CACCACCAGG CCGGGATGGA CATAGAGCCT GTGGAGATCG CCGGTTCCAT CCGAGACTTC 
AGGTTTACTA ACCTATCACT ACATGACGGG GACACGTACT ACGTACGGCT GGTGGCCTGT 
AACGGTGCCA AGCTGTGTAC ATCAGGGCAG ACTCACGGAG TTCTGGTGGA CTCCACCCCT 
CCCACAGTAG GTATGTTAGC CGTACAGACC GACCATGCTG CCCAGTTATG GCGTCACCGT 
GACGGCTGGA TGACGTACCA CCAACAGAAC GGCACCGCCC CGCCTCACGT CAGGTTGGCC 
TGGCTCGGCT TCACGGACAT CCACACTGGG ATCCAGGAAT ATCACGTCAC CATCGGGTCG 
ACTTTTGGTG GCACAGATCT CACGGCAAGT TTTCTCAACA TTAGATATGA CGGAGACAGT 
GTGGTCCTCC CGCACCAGGA CGGCCCGTCC CATGAAGACG AAGGTGCCGT ACAAGTCGCT 
GTAGTGAACC TCACACGGAA CCTGACAGAT AGGGAACACA TCTATGTCAC ACTTTGGGCG 
GTGAACAAGG TTGGATTGCA CAGCATTGTG GTCCATGAAG CCCTGGAAGT GGTTCCGTCT 
AACCCGCACA GCGGCCTCCT GTCATTGGTC AGACGATGTG ACGTACATAC TTGTGAGGGC 
GACTGCACAT GCGCACCACA GAACCAGAAA TGTGCTCCAC TTCTCACCGG ATGTCAAAAC 
GTCGCAAACC GTACAGAGTA TCAGCAGGTT CTTGTGTACG ACCTGACGGA CTTGAGTAGT 
CAAACAGCAC CCGACGCTGA CGTCACACTG TCGCAATGCA CTCTGGGCGC AAGATGGGAA 
ACGGTACCGG GAGGTATACC TGTTCAAAGG TGTGAGTGGA GTGCCGGACA GGAGGGAAAG 
GCAGCAGGTA CCGGGGTGTT TGATTTAAAT AACGACAGGA TCTGGTACGA CGTGGGACTG 
AGAACTTCAG CCGTGCTGAG CATCAGGCGG GACAGACGAG AGGACATTGT TCCCTACACC 
AGTTATAACC TGTCAGACAC CACGAGTGAA GTTCTGATAG ACGGTGTGAC GCTACAGTCC 
GGTGTGAAGT ACTTCAGTAA CGTCCGAGCC TACAGCCATG CCGGCTTACA CACTACAGTC 
AGCTCTGACG GCTTCCTGGT GGATACTGAA CCTCCGTCAG CAGGAATAGT TTACGACGGT 
GCGAACATTC ACGACATGGA CTTTCAGAAC TGCAGTTCTC ACGTGGCCGC TAGCTGGCAC 
GGCTTCTCCG ATCTCCAGTC GTACATCCAC CACTATGTGT GGTGTGTGGG ATCCACGCCT 
GGGACTGACG ATATCCTGAC TTGTACAGAT GTGGGAATAA GTCTGGCGGA AGTGAAAGAG 
ATTTCACCTG CACTTCCAAC TGGTACAAAG TACTTCTCTA CGGTGTACGC TGTGGATGCT 
GCAGGACTGA CGTCATCACC CGCAGTGTCT GATGGGATAC TGGTGGACAC CACTCCACCG 
GAAGTGTTGA ACAAGTTTAA TTTCGGTCCA AACCTGCTGG ATAACCCGTC ATTTGAGAAC 
ATTCAGAGTT CTGCAAGCAC CAACCTTATA TCAGACCTTG GAAGTGCTGG TAACGTCGGC 
CTTACAGGAA CAGACAACAG CAGTGCTAGT AACAGCAGTC ACGTCAATTT ACGTAACAAC 
AGCTTTACAT CAGCTAACAA CGGTGAGCCA GCCCACTGGA ACATCTCCGG TGTAGCGGCC 
GTGATGTCAC CGTCCACGGC TGTGTCTCAC CACGGAGTTT CCTACCTCCT CCTGCACGGG 
TCCATCTCAC AACTCATCCC GACCAGTCCT GGTGAGAGAT ACCGCCTGAC GCTTCACGCC 
TCTCCCATCT TCCCCTCTAA CACACCAGTG GTGGCACAGG AGGGGTACGT CATCAGTCCC 
GGCATGCACC GCGTGTTCAA ACTGTACCAG CGGCCGTCCC ACCTGGAGGG GGCGGAGCTA 
CACGTGGACA GGAACATCAC GTGGCACAAG CACGTGTTCT ACTTCACAGC TGTAGAACAC 
GTGTCTGAGG TAGTCATCGG ATCTGTAGGG CCTTGGAGCG GGATTACCCT GGACGATGTA 
AAGGTCCAAG AGATGCGGCA GGGATCCCGC CCCCCTCCAG CCAACCCCAA CCCGACCAAC 
CAGCTGACCA GTCCTGTCCA CCTGGAGACC AGCTTCGCGC ACGACTGGAC CTCCGTGTGC 
GCCGCCTGGG ACTTTGTGGA CCCTGAGAGT CCGATAGTCG GCTATAGCTG GGCCATCGGT 
ACAGTCAGGG GTGGGACTCA GCTTCAAGAC TTCAAGACAG TCGGGAGAAA CACGCATGCG 
CACAATGAAG ACGTTAGGCT GAGTCATGGA TCGTTTGTTC ACGTGACAGT CGTGGCTGAA 
AACGAGGCCG CTCTTCGTTC TGTTGTGTAC TCGGACCCGG TCCTGGTAGA CCTGACACCT 
CCTGTCATAT CTCTGGTCAA GGCAACACTC TACAGCGCCA CCTTTGACCT TACCGGTCAC 
CTGACCCACG GTGATACTGT CTACTCCACT GTCCGCTGTC ATAACTACGC AGGGATGACG 
TCACACGTGA CGTCAAATGG TGTCACCATT GTCAGTCAGC CGCCAAACAG TGATCAAGCC 
ATAGTGGAAA CTATGAGTGC GCCTCAGTTC TACTACCCGA CCCGAGCCTC CCATCAGTCT 
GCTGCTGACG TCATCCACCT GTCCTGGGAA GGGTTCTTTG ATGTTTCCGG CATCAAGAAT 
TATCAGAGAA TTAATAAGGA CATGTCTTGT ATATGTTTGT TCCTGCATCT GGCGTTCTTA 
TTTACAGCAG GCCGGTTCCA GAGCTGGTGG CCCCGGCAGG GAGTGATGAT GTTCGACTGG 
ACAGGCGTGT TCCTCAGTAA CTCCTCCCTC ATTTACGAGG TGTCCATAGG GACCATGGCA 
GCCGGAAGTG ACGTCATGCA GTGGAGAGAA ACAGACAAGA CCGAGATGCG TCTGGACGTC 
AAAAAGTCAC GTCCCTGTTG TCAACAGGAC GGTAGTGATG AAGAAGACGT GGACTTCCAA 
CAGTCCAAGA CCGTCACTGT CCACTGGAAC GTCACTGATG ATGAGAGTGG GGTAGAGTTC 
TGTGAAGTTG CTATAGGTTT GAGTCCAGGA TCAGGAGAAG TCCACCGGTT TACCCAGCAG 
GCAACACTCT ACAGCGCCAC CTTTGACCTT ACCGGTCACC TGACCCACGG TGATACTGTC 
TACTCCACTG TCCGCTGTCA TAACTACGCA GGGATGACGT CACACGTGAC GTCAAATGGT 
GTCACCATTG TCAGTCAGCC GCCAAACAGT GATCAAGCCA TAGTGGAAAC TATGAGTGCG 
CCTCAGTTCT ACTACCCGAC CCGAGCCTCC CATCAGTCTG CTGCTGACGT CATCCACCTG 
TCCTGGGAAG GGTTCTTTGA TGTTTCCGGC ATCAAGAATT ATCAGTGCCG TGTGACTGGC 
CCTGGTTTGA CTGACTTCCC GTGGATAGAT GTTGGTCTGA CCGGACAGAC TCACGCAACC 
CTGTCAGGGC TCCACCTGCA CAACTACAAC AGGTATGAGG TACACGTCCG AGCTGTCAAT 
CACGTGGGCT TGGTCAGCGA TGACGTCATC TCAGAAATCT ATGTGGAGAT GGAGCGACCA 
CTGCAGGCGG GTTCCAGAGC TGGTGGCCCC AGCAGGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_79472 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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