MEGF8 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MEGF8
Entrez Gene ID	615688
Full Name	multiple EGF like domains 8
General protein information	Preferred Names multiple EGF like domains 8 Names multiple epidermal growth factor-like domains protein 8
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015467508.1	XP_015322994.1	multiple epidermal growth factor-like domains protein 8
XM_024979098.1	XP_024834866.1	multiple epidermal growth factor-like domains protein 8 isoform X1
XM_015458238.1	XP_015313724.1	multiple epidermal growth factor-like domains protein 8
XM_024979099.1	XP_024834867.1	multiple epidermal growth factor-like domains protein 8 isoform X2
XM_024979100.1	XP_024834868.1	multiple epidermal growth factor-like domains protein 8 isoform X3

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LOC709352	XP_002808242.1
Mus musculus (house mouse)	Megf8	NP_001153872.1
Drosophila melanogaster (fruit fly)	CG7466	NP_609180.2
Homo sapiens (human)	MEGF8	NP_001258867.1
Xenopus tropicalis (tropical clawed frog)	megf8	XP_002936442.2
Canis lupus familiaris (dog)	MEGF8	XP_541588.5
Bos taurus (cattle)	MEGF8	XP_002695193.3
Rattus norvegicus (Norway rat)	Megf8	NP_446080.1
Danio rerio (zebrafish)	megf8	XP_005158088.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008185	XP_317282.4
Pan troglodytes (chimpanzee)	MEGF8	XP_003316429.1

Gene Ontology

The following MEGF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEGF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa225577	XM_024979100.1 Latest version!	Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa225575	XM_024979098.1 Latest version!	Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa225576	XM_024979099.1 Latest version!	Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa93525	XM_015467508.1 Latest version!	Bos taurus multiple EGF like domains 8 (MEGF8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa93525	XM_015458238.1 Latest version!	Bos taurus multiple EGF like domains 8 (MEGF8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MEGF8 ( XM_024979100.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834868.1 Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X3, mRNA.

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CloneID	OBa225577
Clone ID Related Accession (Same CDS sequence)	XM_024979100.1
Accession Version	XM_024979100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	multiple epidermal growth factor-like domains protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCC AGCTGTGCCC CTCTCCTCCT TCCCCAGGGG GCAACGTGCA CACCCACTAC 
CAGGAGGAAA AGTGCTATGA AGACGGCATC TTCTTCTACC ACCTGGGCTG CCATCAGTGG 
GTGTCAGGGG CTGAGCTTGC CCCTCCAGGA ACTCCAGAGG GCCGAGCAGC GCCTCCTAGT 
GGCCGGTACT CACACGTGGC GGCTGTGCTT GGTGGCAGTG TCCTGTTGGT GGCCGGGGGG 
TACAGTGGCC GGCCTCGTGG GGACTTGATG GCCTACAAAG TGCCCCCCTT TGTGTTCCAG 
GCGCCTGCTC CAGATTACCA CTTGGACTAT TGCTCCATGT ACACGGACCA CAGCGTCTGC 
TCCCGAGACC CCGAATGCAG TTGGTGTCAG GGGGCCTGCC AAGCCGCCCC ACCTCCTGGG 
ACTCCCTCAG GGGCTTGTCC GGCTGCCAGC TGCCTGGGCC TGAGCCGTCT CCTGGGTGAC 
TGCCAGGCCT GCCTGGCGTT CAGCAGCAGT GCGGCTCCCC CCCAGGGGCC CGGTGCCCTG 
GGCTGGTGCG TGCACAATGA GAGCTGCCTC CCTCGGCCTG AGCAGGCCCG CTGCCGCGGG 
GAGCAGATCT CCGGCACCGT GGGCTGGTGG GGGCCCGCTC CTGTCTTTGT GACCTCCCTG 
GAGGCCTGTG TCACCCAGAG CTTCCTGCCT GGCCTGCACC TGCTCACCTT CCAGCAGCCA 
CCCAACGCCT CCCAGCCTGA CAAGGTCTTG ATCGTCCGCA GCACGACCAT CACCCTGACG 
CCGAGCTCAG AGACCGACGT GTCCCTGGTC TACCGTGGCT TCATCCACCC GCTGCTGCCG 
GGAGGCCCTG GGGGGTCTGG GGCTGAGGAT GTGGCCGTGT GGGCCCGGGC CCAGCGCCTG 
CATGTGCTGG CCCGGATGGC CCGGGGCCCG GACACAGAAA ACATGGAGGA GGTGGGGCGC 
TGGGTGGCAC AGCAGGAGAA GGAGACGAGG CGGCTGCAGC GCCCAGGGTC AGCTCGCCTC 
TTCCCACTGC CCGGCCGGGG CCACAAGTAT GCAGTAGAGA TCCGGGGCCA GCTCAATGGC 
TCAGCAGGCC CCGGGCACAG TGAGCTCACC CTGCTGTGGG ACCGGACTGG CGTGCCAGGT 
GGCAGCGAGA TCTCCTTCTT CTTCCTGGAG CCCTACCGCT CGTCGGCCTG CGCCTCCTAC 
TCCTCCTGCC TGGGCTGCCT GGCAGACCAG GGCTGCGGCT GGTGCCTGGC CAGCGCCACC 
TGCCACCTGC GCCAGAGCGG CGCCCACTGT AGGGACGCTG GGGCCGGCGG GTCCCTGCTC 
GTGCTGGTGC CTGCCCTCTG CCCGCTCTGC GAGGAGCATC GCGACTGCCA CGCCTGCACC 
CAGGACCCCT TCTGTGAGTG GCATCAGAGC ACCAGCCGCA AGGGGGACGC GGCGTGCAGC 
CGGCGCGGCC GGGGTCGGGG CGCCCTGAAG AGTCCGGAAG AGTGCCCCCC ACTCTGCAGC 
CAGCGCCTGA CCTGTGACGA CTGCCTGGCC AACTCGAGCC AGTGCGCCTG GTGCCAGTCC 
ACCCGCACCT GCTTCCTGTT CGCCGCCTAC CTGGCCCGGT ACCCGCACGG GGGCTGCCGA 
GGCTGGGACG ACAGCGTGCA CTCGGAGCCT CGGTGCCGGA GCTGTGACCG CTTCCCGAGC 
TGTCACGAGT GTCTGCAGAG CCATGAGTGT GGCTGGTGTG GAAACGAGGA CAACCCCACG 
CTGGGCCGTT GCCTCCAAGG GGACTTCTCA GGGCCTCTGA GTGGGGGGAA CTGCTCCCTG 
TGGGTCGGGG AGGGCCTGGG GCTGTCTGTG GCCCTCCCCG CCCGCTGGGC GTATGCCCGC 
TGTCCGGATG TAGACGAGTG CCGCCTGGGC CTGGCGCGGT GCCACCTGCG GGCGACCTGC 
CTGAACACAC CCCTCAGCTA CGAGTGTCAC TGCCAGAGGG GCTACCAGGG CGATGGCGTC 
ACTTACTGCA ACCGCACGTG CCTGGAGGAC TGTGGCCACG GTGTGTGCAG CGGCCCCCCA 
GACTTCACCT GCGTGTGTGA CCTGGGCTGG ACATCAGACC TGCCCCTTCC CACGCCGGCC 
CCCGGCCCCC CCGCCCCCCG CTGTTCGCGG GACTGTGGCT GCAACTTCCA CAGCCACTGC 
CACAAGCGGG GGCCCGGCTT CTGCGATGAG TGCCAGGACT GGACGTGGGG GGAGCACTGT 
GAGCGGTGCC GGCCTGGAAG CTTCGGGAAT GCCACAGGCT CCGGCGGCTG CCGGCCCTGC 
CAGTGCAACG GGCATGGGGA CCCTCGCCGA GGCCACTGTG ACAACCTCAG CGGGCTCTGC 
TTCTGCCAGG ACCACACCGA GGGTGCCCAC TGCCAGCTCT GCTCCCCTGG CTACTACGGG 
GACCCTCGGG CAGGTGGCTC CTGCTTCCGG GAGTGTGGGG GTCGTGCCCT CCTCACCAAC 
GTGTCCTCAG TGGCACTGGG CTCACGCCGG TTTGGGGGGC TCCTGCCTGC AGGTGGCGGG 
GCAGCAAGAG CGGGGCCTGG CCTGTCCTAC TGTGTGTGGG TTGTCTCGGC CACTGAGGTG 
CTGCAGCCCT GTGCCCCCGG GACCCTCTGT CCCCCGCTTA CCCTCACCTT CTCCCCCGAC 
AGCAGCACCC CGTGCACGCT GAGCTATGTC CTGGCCTTTG ATGGATTCCC ACGCTTCCTA 
GACACTGGTG TCGTCCAGTC GGACCGTAGC CTCATCGCTG CGTTTTGCGG CCAGCGGCGG 
GACCGGCCCC TCACTGTGCA GGCCCTGTCT GGGCTGCTGG TACTGCACTG GGAGGCCAAT 
GGCTCCTCAT CCTGGGGCTT CAATGCCTCC GTGGGCTCTG CCCGCTGTGG GTCAGGGGGC 
CCCGGGAGCT GCCCTGTCCC CCAGGAGTGT GTGCCCCAGG ATGGGGTCCC AGGTGCTGGA 
CTCTGCCGGT GTCCCCAGGG CTGGGCTGGC CCGCACTGCC GCATGGCTGT GTGTCCTGAG 
AACTGTAATG CCCACAACGG GGCGGGGACG TGTAATCAGA GCCTGGGCGT GTGCGTCTGT 
GCTGAGGGCT TTGGGGGTCC TGACTGTGCC ACGAAGCTGG ATGGTGGGCA GCTGGTCTGG 
GAGACCCTCA TGGACAGCCG CCTCTCAGCT GACACTGCCA GCCGCTTCCT GCACCGCCTG 
GGGCACACGA TGGTGGAGGG ACCTGATGCC ACCTTGTGGA TGTTTGGGGG CCTGGGCCTG 
CCCCAGGGGC TGCTGGGAAA CCTGTATAGG TACTCAGTGA GTGAACGGCG GTGGACACAG 
ATGTTGGCAG GAGCCGAGGA TGGGGGCCCA GGCCCCTCCC CTCGCTCTTT CCACGCTGCT 
GCCTACGTGC CTGCTGGCCG TGGGGCCATG TACCTGCTGG GGGGGCTCAC AGCTGGGGGC 
ATCGCCCGGG ACTTCTGGGT CCTCAACCTC ACCACCCTGC AGTGGCGGCA GGAGAAGGCC 
CCTCAGACTG TGGAGCTGCC AGCCGTTGCT GGTCACACGC TTACTGCCCG CCGAGGCCTA 
TCTCTGCTCC TGGTGGGTGG TTACTCCCCG GAAAATGGCT TCAACCAGCA GCTGCTTGAG 
TACCAGCTGG CGACTGGCAC CTGGGTGTCC GGGGCTCAGA GCGGGACACC CCCCACAGGT 
CTCTACGGTC ACTCTGCTGT GTATCATGAG GCCACCGACT CCCTCTACGT GTTCGGGGGT 
TTCCGCTTCC ACGTGGAGCT GGCAGCCCCG TCCCCTGAGC TCTACTCCCT GCACTGCCCT 
GACCGCACCT GGAGCCTGCT GGCCCCTTCT CAGGGAGCAA AGCCCCGGCC CCGACTCTTC 
CACGCCTCGG CCTTGCTAGG GGACACCATG GTGGTCCTCG GGGGGCGCTC AGACCCCGAC 
GAGTTCAGCA GCGACGTGCT GCTCTACCAG GTCAACTGCA ACACCTGGCT CCTGCCCGAC 
CTCATCGGCC CAGCCTCTGT GGGACCGCCA ATGGAAGAGT CTGTGGCTCA CGCCGTGGCC 
GCGGTAGGGG GTCACCTGTT TATCTCAGGA GGCTTTGGGG GAGTGGCCCT GGGCCGCCTG 
CTGGCCCTGA CCCTGCCCCC TGACCCCTGC CGCCTGCTGC CCTCGCCCGA AGCTTGTAAC 
CAGACTGGGG CCTGTACCTG GTGCCATGGA GCCTGCCTGT CTGGGGACCA GGCCCACAGG 
CTGGGCTGTG GCGGCACCCC CTGCTCCCCT ATGCCCCACT CCCCCGAGGA ATGTCGACGT 
CTGCGGACCT GCAGCGAGTG CCTGGCCCGC CATCCCCGGA CCCTGCAGCC TGGAGATGGA 
GAGGCCTCTG CCCCACGCTG TAAGTGGTGT ACCAACTGCC CCGAGGGTGC CTGCATTGGG 
CGCAACGGGT CCTGCACCTC AGAGAATGAC TGTCGCATCA ACCAGCGCGA GGTCTTCTGG 
GCCGGGAACT GCTCGGAGGC TGTGTGCGGG GCGGCCGACT GCGAGCAGTG CACCAGGGAG 
GGCAAATGCA TGTGGACGCG GCAGTTCAAG AGGACGGGGG AGACCCGCCG CATCCTTTCG 
GTGCAGCCCA CCTACGACTG GACGTGCTTC AGCCACTCAC TGCTGAACGT GTCCCCGATG 
CCAGTGGAGT CATCGCCCCC GCTGCCCTGC CCCACACCCT GTCACCTCCT GCCCAACTGC 
ACCTCCTGCC TGGACTCCAA GGGTGCTGAC GGGGGCTGGC AGCACTGCGT CTGGAGCAGC 
AGCCTCCAGC AGTGTCTGAG CCCCTCCTAT CTGCCCCTGC GGTGTATGGC CGGAGGCTGT 
GGGCGGCTGC TCCGCGGACC CGAGAGCTGC TCCCTGGGCT GTGCTCAGGC AACCCAGTGT 
GCCCTGTGCC TGCGGCGCCC CCACTGTGGC TGGTGTACCT GGGGGGGCCA GGATGGGGGC 
GGCCGCTGCC TGGAGGGTGG ACTCAGTGGC CCCCGTGACG GGCTGACGTG TGGGCGACCT 
GGGGCCTCCT GGGCCTTTCT TTCCTGCCCC CCGGAGGACG AATGTGCCAA CGGGCACCAT 
GACTGCAACG AGACCCAGAA CTGCCACGAC CGGCCCCACG GTTACGAGTG CAGCTGCAAG 
GCAGGCTACA CCATGGACAA CGTGACGGGG CTGTGCCGCC CAGTGTGCGC CCAGGGTTGC 
GTGAACGGCT CGTGTGTGGA GCCCGACCAC TGCCGCTGCC ACTTCGGCTT CGTCGGCCGC 
AACTGTTCCA CGGAGTGTCG CTGCAACCGC CACAGCGACT GCGCCGGCGT GGGGGCCCGC 
GACCACTGCC TGCTCTGCCA CAATCACACC AAGGGCAGCC ACTGTGAGCA GTGCCTCCCG 
CTGTTCGTGG GCTCAGCCGT AGGGGGAGGG ACCTGCCGGC CCTGCCACGC CTTTTGTCGC 
GGAAACAGCC ACGTCTGCGT CTCCAGGAAG GAGCTTGAGA TAGCCAGGAG GGAGCCGGAG 
AAGTACTCGC TGGATCCAGA TGAGATTGAA CACTGGGCGA CAGAGGGTCC CAGCGAAGAT 
GAGGCTGTGT GTGTGAACTG CCAGAATAAC AGCTATGGGG ATAAGTGTGA GAGCTGCCTC 
CACGGCTACT TCCTCCTCGA CGGGAAATGC ACCAAATGCC AGTGTAATGG CCACGCGGAC 
ACATGTAATG AGCAGGATGG GACAGGCTGC CCATGTCAGA ACAACACAGA GACAGGCACG 
TGCCAGGGCA GCTCCCCCAG TGACCGCCGA GACTGCTACA AATACCAGTG TGCCAAGTGC 
CGAGAGTCAT TCCACGGGAG CCCGCTAGGG GGCCAGCAGT GCTACCGCCT CATCTCCGTG 
GAACAGGAGT ACTGCCTGGA CCCCACGTCC CAAACCAACT GTTTCCACGA GCCCAAGCGC 
CGGGCTCTGG GTCCCGGACG CACCGTTCTC TTTGGAGTGC AGCCTAAGTT CACCAACGTG 
GACATCCGCC TGACGCTGGA CGTGACCTTT GGCGCCGTGG ACCTCTACGT CTCCACCTCC 
TATGACACCT TCGTGGTCCG CGTGGCTCCC GACACTGGTG TCCACACCGT GCATATCCAG 
CCGCCACCAC CTCCCCCACC CCCACCACCC CCTGCTGACG GCGGGCCCCG GGGACCTGGG 
GATCTCGGGG GACCTGGGGC TGGAGGTGGG CCGGCTACCC CAGCAGAGCC GCGAGTGCGG 
GAGGTCTGGC CACGGGGCCT GATCACCTAC GTGACAGTGA CAGAGCCGTC GGCGGTGCTG 
GTGGTCCGTG GCGTGAGGGA CCGGCTGGTC ATCACCTACC CGCACGAGCA CCACACCCTC 
AAGTCCAGCC GCTTCTACCT GCTCCTGCTG GGTGTGGGAG ACCCAAGTGG GCCCAGTGCC 
AACGGCTCAG CTGACTCGCA GGGCTTGCTG TTCTTCCGGC AGGACCAGGC CCACATTGAC 
CTGTTTGTCT TCTTCTCTGT CTTCTTCTCC TGCTTCTTCC TCTTCCTCTC GCTCTGTGTG 
CTGCTCTGGA AGGCCAAGCA GGCCCTGGAC CACCGGCAGG AACAGCGCCG GCACCTGCAG 
GAGATGACCA AGATGGCCAG CCGCCCCTTC GCCAAGGTCA CTGTCTGCTT CCCGCCAGAC 
CCTGCTGCCC CAGCCCCCAC CTGGAAGCCT GCCGGGCTCC CGCCACCCGC CTTCCGCCGC 
TCCGAGCCCT TCTTGGCACC CTTGCTGCTG ACCGGGGCCG GCGGGCCCTG GGGACCTGTA 
GGAGGGGGCT GCTGCCCACC GGCCATCCCC ACCACCACTG CCGGCCTGCG AGCCGGGCCT 
ATCACCCTTG AGCCCACCGA GGATGGCATG GCCGGCGTGG CCACACTGCT GCTCCAACTG 
CCTGGCGGGC CCCACGCACC CAACGGCGCC TGCCTGGGGT CAGCGCTCGT CACACTGCGG 
CACCGGCTGC ACGAATACTG CGGCGGCGGC GGGGGCGCTG GGGGCAGTGG GCATGGGGCT 
GGTGCGGGCC GGAAGGGGCT GTTGAGCCAG GACAACCTCA CCAGCATGTC CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834868.1
CDS	127..7143
Translation	MAGQLCPSPPSPGGNVHTHYQEEKCYEDGIFFYHLGCHQWVSGAELAPPGTPEGRAAPPSGRYSHVAAVLGGSVLLVAGGYSGRPRGDLMAYKVPPFVFQAPAPDYHLDYCSMYTDHSVCSRDPECSWCQGACQAAPPPGTPSGACPAASCLGLSRLLGDCQACLAFSSSAAPPQGPGALGWCVHNESCLPRPEQARCRGEQISGTVGWWGPAPVFVTSLEACVTQSFLPGLHLLTFQQPPNASQPDKVLIVRSTTITLTPSSETDVSLVYRGFIHPLLPGGPGGSGAEDVAVWARAQRLHVLARMARGPDTENMEEVGRWVAQQEKETRRLQRPGSARLFPLPGRGHKYAVEIRGQLNGSAGPGHSELTLLWDRTGVPGGSEISFFFLEPYRSSACASYSSCLGCLADQGCGWCLASATCHLRQSGAHCRDAGAGGSLLVLVPALCPLCEEHRDCHACTQDPFCEWHQSTSRKGDAACSRRGRGRGALKSPEECPPLCSQRLTCDDCLANSSQCAWCQSTRTCFLFAAYLARYPHGGCRGWDDSVHSEPRCRSCDRFPSCHECLQSHECGWCGNEDNPTLGRCLQGDFSGPLSGGNCSLWVGEGLGLSVALPARWAYARCPDVDECRLGLARCHLRATCLNTPLSYECHCQRGYQGDGVTYCNRTCLEDCGHGVCSGPPDFTCVCDLGWTSDLPLPTPAPGPPAPRCSRDCGCNFHSHCHKRGPGFCDECQDWTWGEHCERCRPGSFGNATGSGGCRPCQCNGHGDPRRGHCDNLSGLCFCQDHTEGAHCQLCSPGYYGDPRAGGSCFRECGGRALLTNVSSVALGSRRFGGLLPAGGGAARAGPGLSYCVWVVSATEVLQPCAPGTLCPPLTLTFSPDSSTPCTLSYVLAFDGFPRFLDTGVVQSDRSLIAAFCGQRRDRPLTVQALSGLLVLHWEANGSSSWGFNASVGSARCGSGGPGSCPVPQECVPQDGVPGAGLCRCPQGWAGPHCRMAVCPENCNAHNGAGTCNQSLGVCVCAEGFGGPDCATKLDGGQLVWETLMDSRLSADTASRFLHRLGHTMVEGPDATLWMFGGLGLPQGLLGNLYRYSVSERRWTQMLAGAEDGGPGPSPRSFHAAAYVPAGRGAMYLLGGLTAGGIARDFWVLNLTTLQWRQEKAPQTVELPAVAGHTLTARRGLSLLLVGGYSPENGFNQQLLEYQLATGTWVSGAQSGTPPTGLYGHSAVYHEATDSLYVFGGFRFHVELAAPSPELYSLHCPDRTWSLLAPSQGAKPRPRLFHASALLGDTMVVLGGRSDPDEFSSDVLLYQVNCNTWLLPDLIGPASVGPPMEESVAHAVAAVGGHLFISGGFGGVALGRLLALTLPPDPCRLLPSPEACNQTGACTWCHGACLSGDQAHRLGCGGTPCSPMPHSPEECRRLRTCSECLARHPRTLQPGDGEASAPRCKWCTNCPEGACIGRNGSCTSENDCRINQREVFWAGNCSEAVCGAADCEQCTREGKCMWTRQFKRTGETRRILSVQPTYDWTCFSHSLLNVSPMPVESSPPLPCPTPCHLLPNCTSCLDSKGADGGWQHCVWSSSLQQCLSPSYLPLRCMAGGCGRLLRGPESCSLGCAQATQCALCLRRPHCGWCTWGGQDGGGRCLEGGLSGPRDGLTCGRPGASWAFLSCPPEDECANGHHDCNETQNCHDRPHGYECSCKAGYTMDNVTGLCRPVCAQGCVNGSCVEPDHCRCHFGFVGRNCSTECRCNRHSDCAGVGARDHCLLCHNHTKGSHCEQCLPLFVGSAVGGGTCRPCHAFCRGNSHVCVSRKELEIARREPEKYSLDPDEIEHWATEGPSEDEAVCVNCQNNSYGDKCESCLHGYFLLDGKCTKCQCNGHADTCNEQDGTGCPCQNNTETGTCQGSSPSDRRDCYKYQCAKCRESFHGSPLGGQQCYRLISVEQEYCLDPTSQTNCFHEPKRRALGPGRTVLFGVQPKFTNVDIRLTLDVTFGAVDLYVSTSYDTFVVRVAPDTGVHTVHIQPPPPPPPPPPPADGGPRGPGDLGGPGAGGGPATPAEPRVREVWPRGLITYVTVTEPSAVLVVRGVRDRLVITYPHEHHTLKSSRFYLLLLGVGDPSGPSANGSADSQGLLFFRQDQAHIDLFVFFSVFFSCFFLFLSLCVLLWKAKQALDHRQEQRRHLQEMTKMASRPFAKVTVCFPPDPAAPAPTWKPAGLPPPAFRRSEPFLAPLLLTGAGGPWGPVGGGCCPPAIPTTTAGLRAGPITLEPTEDGMAGVATLLLQLPGGPHAPNGACLGSALVTLRHRLHEYCGGGGGAGGSGHGAGAGRKGLLSQDNLTSMSL

Target ORF information:

RefSeq Version	XM_024979100.1
Organism	Bos taurus(cattle)
Definition	Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979100.1

ORF Insert Sequence:

ATGGCTGGCC AGCTGTGCCC CTCTCCTCCT TCCCCAGGGG GCAACGTGCA CACCCACTAC 
CAGGAGGAAA AGTGCTATGA AGACGGCATC TTCTTCTACC ACCTGGGCTG CCATCAGTGG 
GTGTCAGGGG CTGAGCTTGC CCCTCCAGGA ACTCCAGAGG GCCGAGCAGC GCCTCCTAGT 
GGCCGGTACT CACACGTGGC GGCTGTGCTT GGTGGCAGTG TCCTGTTGGT GGCCGGGGGG 
TACAGTGGCC GGCCTCGTGG GGACTTGATG GCCTACAAAG TGCCCCCCTT TGTGTTCCAG 
GCGCCTGCTC CAGATTACCA CTTGGACTAT TGCTCCATGT ACACGGACCA CAGCGTCTGC 
TCCCGAGACC CCGAATGCAG TTGGTGTCAG GGGGCCTGCC AAGCCGCCCC ACCTCCTGGG 
ACTCCCTCAG GGGCTTGTCC GGCTGCCAGC TGCCTGGGCC TGAGCCGTCT CCTGGGTGAC 
TGCCAGGCCT GCCTGGCGTT CAGCAGCAGT GCGGCTCCCC CCCAGGGGCC CGGTGCCCTG 
GGCTGGTGCG TGCACAATGA GAGCTGCCTC CCTCGGCCTG AGCAGGCCCG CTGCCGCGGG 
GAGCAGATCT CCGGCACCGT GGGCTGGTGG GGGCCCGCTC CTGTCTTTGT GACCTCCCTG 
GAGGCCTGTG TCACCCAGAG CTTCCTGCCT GGCCTGCACC TGCTCACCTT CCAGCAGCCA 
CCCAACGCCT CCCAGCCTGA CAAGGTCTTG ATCGTCCGCA GCACGACCAT CACCCTGACG 
CCGAGCTCAG AGACCGACGT GTCCCTGGTC TACCGTGGCT TCATCCACCC GCTGCTGCCG 
GGAGGCCCTG GGGGGTCTGG GGCTGAGGAT GTGGCCGTGT GGGCCCGGGC CCAGCGCCTG 
CATGTGCTGG CCCGGATGGC CCGGGGCCCG GACACAGAAA ACATGGAGGA GGTGGGGCGC 
TGGGTGGCAC AGCAGGAGAA GGAGACGAGG CGGCTGCAGC GCCCAGGGTC AGCTCGCCTC 
TTCCCACTGC CCGGCCGGGG CCACAAGTAT GCAGTAGAGA TCCGGGGCCA GCTCAATGGC 
TCAGCAGGCC CCGGGCACAG TGAGCTCACC CTGCTGTGGG ACCGGACTGG CGTGCCAGGT 
GGCAGCGAGA TCTCCTTCTT CTTCCTGGAG CCCTACCGCT CGTCGGCCTG CGCCTCCTAC 
TCCTCCTGCC TGGGCTGCCT GGCAGACCAG GGCTGCGGCT GGTGCCTGGC CAGCGCCACC 
TGCCACCTGC GCCAGAGCGG CGCCCACTGT AGGGACGCTG GGGCCGGCGG GTCCCTGCTC 
GTGCTGGTGC CTGCCCTCTG CCCGCTCTGC GAGGAGCATC GCGACTGCCA CGCCTGCACC 
CAGGACCCCT TCTGTGAGTG GCATCAGAGC ACCAGCCGCA AGGGGGACGC GGCGTGCAGC 
CGGCGCGGCC GGGGTCGGGG CGCCCTGAAG AGTCCGGAAG AGTGCCCCCC ACTCTGCAGC 
CAGCGCCTGA CCTGTGACGA CTGCCTGGCC AACTCGAGCC AGTGCGCCTG GTGCCAGTCC 
ACCCGCACCT GCTTCCTGTT CGCCGCCTAC CTGGCCCGGT ACCCGCACGG GGGCTGCCGA 
GGCTGGGACG ACAGCGTGCA CTCGGAGCCT CGGTGCCGGA GCTGTGACCG CTTCCCGAGC 
TGTCACGAGT GTCTGCAGAG CCATGAGTGT GGCTGGTGTG GAAACGAGGA CAACCCCACG 
CTGGGCCGTT GCCTCCAAGG GGACTTCTCA GGGCCTCTGA GTGGGGGGAA CTGCTCCCTG 
TGGGTCGGGG AGGGCCTGGG GCTGTCTGTG GCCCTCCCCG CCCGCTGGGC GTATGCCCGC 
TGTCCGGATG TAGACGAGTG CCGCCTGGGC CTGGCGCGGT GCCACCTGCG GGCGACCTGC 
CTGAACACAC CCCTCAGCTA CGAGTGTCAC TGCCAGAGGG GCTACCAGGG CGATGGCGTC 
ACTTACTGCA ACCGCACGTG CCTGGAGGAC TGTGGCCACG GTGTGTGCAG CGGCCCCCCA 
GACTTCACCT GCGTGTGTGA CCTGGGCTGG ACATCAGACC TGCCCCTTCC CACGCCGGCC 
CCCGGCCCCC CCGCCCCCCG CTGTTCGCGG GACTGTGGCT GCAACTTCCA CAGCCACTGC 
CACAAGCGGG GGCCCGGCTT CTGCGATGAG TGCCAGGACT GGACGTGGGG GGAGCACTGT 
GAGCGGTGCC GGCCTGGAAG CTTCGGGAAT GCCACAGGCT CCGGCGGCTG CCGGCCCTGC 
CAGTGCAACG GGCATGGGGA CCCTCGCCGA GGCCACTGTG ACAACCTCAG CGGGCTCTGC 
TTCTGCCAGG ACCACACCGA GGGTGCCCAC TGCCAGCTCT GCTCCCCTGG CTACTACGGG 
GACCCTCGGG CAGGTGGCTC CTGCTTCCGG GAGTGTGGGG GTCGTGCCCT CCTCACCAAC 
GTGTCCTCAG TGGCACTGGG CTCACGCCGG TTTGGGGGGC TCCTGCCTGC AGGTGGCGGG 
GCAGCAAGAG CGGGGCCTGG CCTGTCCTAC TGTGTGTGGG TTGTCTCGGC CACTGAGGTG 
CTGCAGCCCT GTGCCCCCGG GACCCTCTGT CCCCCGCTTA CCCTCACCTT CTCCCCCGAC 
AGCAGCACCC CGTGCACGCT GAGCTATGTC CTGGCCTTTG ATGGATTCCC ACGCTTCCTA 
GACACTGGTG TCGTCCAGTC GGACCGTAGC CTCATCGCTG CGTTTTGCGG CCAGCGGCGG 
GACCGGCCCC TCACTGTGCA GGCCCTGTCT GGGCTGCTGG TACTGCACTG GGAGGCCAAT 
GGCTCCTCAT CCTGGGGCTT CAATGCCTCC GTGGGCTCTG CCCGCTGTGG GTCAGGGGGC 
CCCGGGAGCT GCCCTGTCCC CCAGGAGTGT GTGCCCCAGG ATGGGGTCCC AGGTGCTGGA 
CTCTGCCGGT GTCCCCAGGG CTGGGCTGGC CCGCACTGCC GCATGGCTGT GTGTCCTGAG 
AACTGTAATG CCCACAACGG GGCGGGGACG TGTAATCAGA GCCTGGGCGT GTGCGTCTGT 
GCTGAGGGCT TTGGGGGTCC TGACTGTGCC ACGAAGCTGG ATGGTGGGCA GCTGGTCTGG 
GAGACCCTCA TGGACAGCCG CCTCTCAGCT GACACTGCCA GCCGCTTCCT GCACCGCCTG 
GGGCACACGA TGGTGGAGGG ACCTGATGCC ACCTTGTGGA TGTTTGGGGG CCTGGGCCTG 
CCCCAGGGGC TGCTGGGAAA CCTGTATAGG TACTCAGTGA GTGAACGGCG GTGGACACAG 
ATGTTGGCAG GAGCCGAGGA TGGGGGCCCA GGCCCCTCCC CTCGCTCTTT CCACGCTGCT 
GCCTACGTGC CTGCTGGCCG TGGGGCCATG TACCTGCTGG GGGGGCTCAC AGCTGGGGGC 
ATCGCCCGGG ACTTCTGGGT CCTCAACCTC ACCACCCTGC AGTGGCGGCA GGAGAAGGCC 
CCTCAGACTG TGGAGCTGCC AGCCGTTGCT GGTCACACGC TTACTGCCCG CCGAGGCCTA 
TCTCTGCTCC TGGTGGGTGG TTACTCCCCG GAAAATGGCT TCAACCAGCA GCTGCTTGAG 
TACCAGCTGG CGACTGGCAC CTGGGTGTCC GGGGCTCAGA GCGGGACACC CCCCACAGGT 
CTCTACGGTC ACTCTGCTGT GTATCATGAG GCCACCGACT CCCTCTACGT GTTCGGGGGT 
TTCCGCTTCC ACGTGGAGCT GGCAGCCCCG TCCCCTGAGC TCTACTCCCT GCACTGCCCT 
GACCGCACCT GGAGCCTGCT GGCCCCTTCT CAGGGAGCAA AGCCCCGGCC CCGACTCTTC 
CACGCCTCGG CCTTGCTAGG GGACACCATG GTGGTCCTCG GGGGGCGCTC AGACCCCGAC 
GAGTTCAGCA GCGACGTGCT GCTCTACCAG GTCAACTGCA ACACCTGGCT CCTGCCCGAC 
CTCATCGGCC CAGCCTCTGT GGGACCGCCA ATGGAAGAGT CTGTGGCTCA CGCCGTGGCC 
GCGGTAGGGG GTCACCTGTT TATCTCAGGA GGCTTTGGGG GAGTGGCCCT GGGCCGCCTG 
CTGGCCCTGA CCCTGCCCCC TGACCCCTGC CGCCTGCTGC CCTCGCCCGA AGCTTGTAAC 
CAGACTGGGG CCTGTACCTG GTGCCATGGA GCCTGCCTGT CTGGGGACCA GGCCCACAGG 
CTGGGCTGTG GCGGCACCCC CTGCTCCCCT ATGCCCCACT CCCCCGAGGA ATGTCGACGT 
CTGCGGACCT GCAGCGAGTG CCTGGCCCGC CATCCCCGGA CCCTGCAGCC TGGAGATGGA 
GAGGCCTCTG CCCCACGCTG TAAGTGGTGT ACCAACTGCC CCGAGGGTGC CTGCATTGGG 
CGCAACGGGT CCTGCACCTC AGAGAATGAC TGTCGCATCA ACCAGCGCGA GGTCTTCTGG 
GCCGGGAACT GCTCGGAGGC TGTGTGCGGG GCGGCCGACT GCGAGCAGTG CACCAGGGAG 
GGCAAATGCA TGTGGACGCG GCAGTTCAAG AGGACGGGGG AGACCCGCCG CATCCTTTCG 
GTGCAGCCCA CCTACGACTG GACGTGCTTC AGCCACTCAC TGCTGAACGT GTCCCCGATG 
CCAGTGGAGT CATCGCCCCC GCTGCCCTGC CCCACACCCT GTCACCTCCT GCCCAACTGC 
ACCTCCTGCC TGGACTCCAA GGGTGCTGAC GGGGGCTGGC AGCACTGCGT CTGGAGCAGC 
AGCCTCCAGC AGTGTCTGAG CCCCTCCTAT CTGCCCCTGC GGTGTATGGC CGGAGGCTGT 
GGGCGGCTGC TCCGCGGACC CGAGAGCTGC TCCCTGGGCT GTGCTCAGGC AACCCAGTGT 
GCCCTGTGCC TGCGGCGCCC CCACTGTGGC TGGTGTACCT GGGGGGGCCA GGATGGGGGC 
GGCCGCTGCC TGGAGGGTGG ACTCAGTGGC CCCCGTGACG GGCTGACGTG TGGGCGACCT 
GGGGCCTCCT GGGCCTTTCT TTCCTGCCCC CCGGAGGACG AATGTGCCAA CGGGCACCAT 
GACTGCAACG AGACCCAGAA CTGCCACGAC CGGCCCCACG GTTACGAGTG CAGCTGCAAG 
GCAGGCTACA CCATGGACAA CGTGACGGGG CTGTGCCGCC CAGTGTGCGC CCAGGGTTGC 
GTGAACGGCT CGTGTGTGGA GCCCGACCAC TGCCGCTGCC ACTTCGGCTT CGTCGGCCGC 
AACTGTTCCA CGGAGTGTCG CTGCAACCGC CACAGCGACT GCGCCGGCGT GGGGGCCCGC 
GACCACTGCC TGCTCTGCCA CAATCACACC AAGGGCAGCC ACTGTGAGCA GTGCCTCCCG 
CTGTTCGTGG GCTCAGCCGT AGGGGGAGGG ACCTGCCGGC CCTGCCACGC CTTTTGTCGC 
GGAAACAGCC ACGTCTGCGT CTCCAGGAAG GAGCTTGAGA TAGCCAGGAG GGAGCCGGAG 
AAGTACTCGC TGGATCCAGA TGAGATTGAA CACTGGGCGA CAGAGGGTCC CAGCGAAGAT 
GAGGCTGTGT GTGTGAACTG CCAGAATAAC AGCTATGGGG ATAAGTGTGA GAGCTGCCTC 
CACGGCTACT TCCTCCTCGA CGGGAAATGC ACCAAATGCC AGTGTAATGG CCACGCGGAC 
ACATGTAATG AGCAGGATGG GACAGGCTGC CCATGTCAGA ACAACACAGA GACAGGCACG 
TGCCAGGGCA GCTCCCCCAG TGACCGCCGA GACTGCTACA AATACCAGTG TGCCAAGTGC 
CGAGAGTCAT TCCACGGGAG CCCGCTAGGG GGCCAGCAGT GCTACCGCCT CATCTCCGTG 
GAACAGGAGT ACTGCCTGGA CCCCACGTCC CAAACCAACT GTTTCCACGA GCCCAAGCGC 
CGGGCTCTGG GTCCCGGACG CACCGTTCTC TTTGGAGTGC AGCCTAAGTT CACCAACGTG 
GACATCCGCC TGACGCTGGA CGTGACCTTT GGCGCCGTGG ACCTCTACGT CTCCACCTCC 
TATGACACCT TCGTGGTCCG CGTGGCTCCC GACACTGGTG TCCACACCGT GCATATCCAG 
CCGCCACCAC CTCCCCCACC CCCACCACCC CCTGCTGACG GCGGGCCCCG GGGACCTGGG 
GATCTCGGGG GACCTGGGGC TGGAGGTGGG CCGGCTACCC CAGCAGAGCC GCGAGTGCGG 
GAGGTCTGGC CACGGGGCCT GATCACCTAC GTGACAGTGA CAGAGCCGTC GGCGGTGCTG 
GTGGTCCGTG GCGTGAGGGA CCGGCTGGTC ATCACCTACC CGCACGAGCA CCACACCCTC 
AAGTCCAGCC GCTTCTACCT GCTCCTGCTG GGTGTGGGAG ACCCAAGTGG GCCCAGTGCC 
AACGGCTCAG CTGACTCGCA GGGCTTGCTG TTCTTCCGGC AGGACCAGGC CCACATTGAC 
CTGTTTGTCT TCTTCTCTGT CTTCTTCTCC TGCTTCTTCC TCTTCCTCTC GCTCTGTGTG 
CTGCTCTGGA AGGCCAAGCA GGCCCTGGAC CACCGGCAGG AACAGCGCCG GCACCTGCAG 
GAGATGACCA AGATGGCCAG CCGCCCCTTC GCCAAGGTCA CTGTCTGCTT CCCGCCAGAC 
CCTGCTGCCC CAGCCCCCAC CTGGAAGCCT GCCGGGCTCC CGCCACCCGC CTTCCGCCGC 
TCCGAGCCCT TCTTGGCACC CTTGCTGCTG ACCGGGGCCG GCGGGCCCTG GGGACCTGTA 
GGAGGGGGCT GCTGCCCACC GGCCATCCCC ACCACCACTG CCGGCCTGCG AGCCGGGCCT 
ATCACCCTTG AGCCCACCGA GGATGGCATG GCCGGCGTGG CCACACTGCT GCTCCAACTG 
CCTGGCGGGC CCCACGCACC CAACGGCGCC TGCCTGGGGT CAGCGCTCGT CACACTGCGG 
CACCGGCTGC ACGAATACTG CGGCGGCGGC GGGGGCGCTG GGGGCAGTGG GCATGGGGCT 
GGTGCGGGCC GGAAGGGGCT GTTGAGCCAG GACAACCTCA CCAGCATGTC CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF8 ( XM_024979098.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834866.1 Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X1, mRNA.

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CloneID	OBa225575
Clone ID Related Accession (Same CDS sequence)	XM_024979098.1
Accession Version	XM_024979098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	multiple epidermal growth factor-like domains protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTGTAT CATTGGCCGT GCTGGGGCCA 
CTGTCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT 
CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC 
ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA 
TGCACTTATG ACTACCTGTT CGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC 
AGTCTGAGTG GGAGCACACG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTG 
CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC 
CTGTGCCCGG GTGGCTGCCG CAGCCATGGG CAGTGCCAGC CCCCAGGGGT GTGTGTCTGT 
GAGCCGGGCT GGGGGGGCCC CGACTGCAGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC 
CATGGCACCT GCACCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGC 
GCCTGTGACC TGCACCTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC 
GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACTGGGG 
CTGCTGGCTG TTTTTGGAGG CCAGGACCTC AACCGCGCGC TGGGGGACCT CGTCCTGTAC 
AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC 
TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT 
GGCACCCTCA CCAGCGACAT CTGGGCCTTT AGCCCGATGG GTGGCGGCCG CTGGGAGCTT 
CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA TGCAGCAGCC 
CTGGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC 
TCCGGCCTCT TCCGTTTCCG CCTCGACGGC ACCAGTGGGG GCTATTGGGA GCAGGTGATG 
CCAGCAGGTG GGCGGCCCCC TGCCGCCACC GGCCATTCCA TGGTGTTCCA TGCCGCCTCC 
CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG 
AACTCCACCG AGCTCTTCCA TGTGGATCGG CGCGTGTGGA CCACCCTGAA GGGCCGGGAG 
GGACTTCACG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG 
CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC 
ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCTGAGCT TGCCCCTCCA 
GGAACTCCAG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG 
CTTGGTGGCA GTGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCTCG TGGGGACTTG 
ATGGCCTACA AAGTGCCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGATTA CCACTTGGAC 
TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGTTGGTGT 
CAGGGGGCCT GCCAAGCCGC CCCACCTCCT GGGACTCCCT CAGGGGCTTG TCCGGCTGCC 
AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC 
AGTGCGGCTC CCCCCCAGGG GCCCGGTGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC 
CTCCCTCGGC CTGAGCAGGC CCGCTGCCGC GGGGAGCAGA TCTCCGGCAC CGTGGGCTGG 
TGGGGGCCCG CTCCTGTCTT TGTGACCTCC CTGGAGGCCT GTGTCACCCA GAGCTTCCTG 
CCTGGCCTGC ACCTGCTCAC CTTCCAGCAG CCACCCAACG CCTCCCAGCC TGACAAGGTC 
TTGATCGTCC GCAGCACGAC CATCACCCTG ACGCCGAGCT CAGAGACCGA CGTGTCCCTG 
GTCTACCGTG GCTTCATCCA CCCGCTGCTG CCGGGAGGCC CTGGGGGGTC TGGGGCTGAG 
GATGTGGCCG TGTGGGCCCG GGCCCAGCGC CTGCATGTGC TGGCCCGGAT GGCCCGGGGC 
CCGGACACAG AAAACATGGA GGAGGTGGGG CGCTGGGTGG CACAGCAGGA GAAGGAGACG 
AGGCGGCTGC AGCGCCCAGG GTCAGCTCGC CTCTTCCCAC TGCCCGGCCG GGGCCACAAG 
TATGCAGTAG AGATCCGGGG CCAGCTCAAT GGCTCAGCAG GCCCCGGGCA CAGTGAGCTC 
ACCCTGCTGT GGGACCGGAC TGGCGTGCCA GGTGGCAGCG AGATCTCCTT CTTCTTCCTG 
GAGCCCTACC GCTCGTCGGC CTGCGCCTCC TACTCCTCCT GCCTGGGCTG CCTGGCAGAC 
CAGGGCTGCG GCTGGTGCCT GGCCAGCGCC ACCTGCCACC TGCGCCAGAG CGGCGCCCAC 
TGTAGGGACG CTGGGGCCGG CGGGTCCCTG CTCGTGCTGG TGCCTGCCCT CTGCCCGCTC 
TGCGAGGAGC ATCGCGACTG CCACGCCTGC ACCCAGGACC CCTTCTGTGA GTGGCATCAG 
AGCACCAGCC GCAAGGGGGA CGCGGCGTGC AGCCGGCGCG GCCGGGGTCG GGGCGCCCTG 
AAGAGTCCGG AAGAGTGCCC CCCACTCTGC AGCCAGCGCC TGACCTGTGA CGACTGCCTG 
GCCAACTCGA GCCAGTGCGC CTGGTGCCAG TCCACCCGCA CCTGCTTCCT GTTCGCCGCC 
TACCTGGCCC GGTACCCGCA CGGGGGCTGC CGAGGCTGGG ACGACAGCGT GCACTCGGAG 
CCTCGGTGCC GGAGCTGTGA CCGCTTCCCG AGCTGTCACG AGTGTCTGCA GAGCCATGAG 
TGTGGCTGGT GTGGAAACGA GGACAACCCC ACGCTGGGCC GTTGCCTCCA AGGGGACTTC 
TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT 
GTGGCCCTCC CCGCCCGCTG GGCGTATGCC CGCTGTCCGG ATGTAGACGA GTGCCGCCTG 
GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTACGAGTGT 
CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG 
GACTGTGGCC ACGGTGTGTG CAGCGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC 
TGGACATCAG ACCTGCCCCT TCCCACGCCG GCCCCCGGCC CCCCCGCCCC CCGCTGTTCG 
CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAT 
GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCTGG AAGCTTCGGG 
AATGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ACGGGCATGG GGACCCTCGC 
CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC 
CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC 
CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC 
CGGTTTGGGG GGCTCCTGCC TGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC 
TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTGCAGC CCTGTGCCCC CGGGACCCTC 
TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAT 
GTCCTGGCCT TTGATGGATT CCCACGCTTC CTAGACACTG GTGTCGTCCA GTCGGACCGT 
AGCCTCATCG CTGCGTTTTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG 
TCTGGGCTGC TGGTACTGCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAATGCC 
TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCCGGGA GCTGCCCTGT CCCCCAGGAG 
TGTGTGCCCC AGGATGGGGT CCCAGGTGCT GGACTCTGCC GGTGTCCCCA GGGCTGGGCT 
GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG 
ACGTGTAATC AGAGCCTGGG CGTGTGCGTC TGTGCTGAGG GCTTTGGGGG TCCTGACTGT 
GCCACGAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA 
GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT 
GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT 
AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCCGA GGATGGGGGC 
CCAGGCCCCT CCCCTCGCTC TTTCCACGCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC 
ATGTACCTGC TGGGGGGGCT CACAGCTGGG GGCATCGCCC GGGACTTCTG GGTCCTCAAC 
CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCCGTT 
GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC 
CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG 
TCCGGGGCTC AGAGCGGGAC ACCCCCCACA GGTCTCTACG GTCACTCTGC TGTGTATCAT 
GAGGCCACCG ACTCCCTCTA CGTGTTCGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC 
CCGTCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT 
TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCACGCCT CGGCCTTGCT AGGGGACACC 
ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGCGACGT GCTGCTCTAC 
CAGGTCAACT GCAACACCTG GCTCCTGCCC GACCTCATCG GCCCAGCCTC TGTGGGACCG 
CCAATGGAAG AGTCTGTGGC TCACGCCGTG GCCGCGGTAG GGGGTCACCT GTTTATCTCA 
GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCC TGACCCTGCC CCCTGACCCC 
TGCCGCCTGC TGCCCTCGCC CGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT 
GGAGCCTGCC TGTCTGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCAC CCCCTGCTCC 
CCTATGCCCC ACTCCCCCGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC 
CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCT CTGCCCCACG CTGTAAGTGG 
TGTACCAACT GCCCCGAGGG TGCCTGCATT GGGCGCAACG GGTCCTGCAC CTCAGAGAAT 
GACTGTCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCGGA GGCTGTGTGC 
GGGGCGGCCG ACTGCGAGCA GTGCACCAGG GAGGGCAAAT GCATGTGGAC GCGGCAGTTC 
AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC 
TTCAGCCACT CACTGCTGAA CGTGTCCCCG ATGCCAGTGG AGTCATCGCC CCCGCTGCCC 
TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT 
GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC 
TATCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC 
TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGTGCCCTGT GCCTGCGGCG CCCCCACTGT 
GGCTGGTGTA CCTGGGGGGG CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT 
GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CCTGGGCCTT TCTTTCCTGC 
CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ACGAGACCCA GAACTGCCAC 
GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG 
GGGCTGTGCC GCCCAGTGTG CGCCCAGGGT TGCGTGAACG GCTCGTGTGT GGAGCCCGAC 
CACTGCCGCT GCCACTTCGG CTTCGTCGGC CGCAACTGTT CCACGGAGTG TCGCTGCAAC 
CGCCACAGCG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAATCAC 
ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCGCTGTTCG TGGGCTCAGC CGTAGGGGGA 
GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGAAACA GCCACGTCTG CGTCTCCAGG 
AAGGAGCTTG AGATAGCCAG GAGGGAGCCG GAGAAGTACT CGCTGGATCC AGATGAGATT 
GAACACTGGG CGACAGAGGG TCCCAGCGAA GATGAGGCTG TGTGTGTGAA CTGCCAGAAT 
AACAGCTATG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTCCTCCT CGACGGGAAA 
TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACATGTA ATGAGCAGGA TGGGACAGGC 
TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC 
CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA 
GGGGGCCAGC AGTGCTACCG CCTCATCTCC GTGGAACAGG AGTACTGCCT GGACCCCACG 
TCCCAAACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGTCCCGG ACGCACCGTT 
CTCTTTGGAG TGCAGCCTAA GTTCACCAAC GTGGACATCC GCCTGACGCT GGACGTGACC 
TTTGGCGCCG TGGACCTCTA CGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT 
CCCGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CACCTCCCCC ACCCCCACCA 
CCCCCTGCTG ACGGCGGGCC CCGGGGACCT GGGGATCTCG GGGGACCTGG GGCTGGAGGT 
GGGCCGGCTA CCCCAGCAGA GCCGCGAGTG CGGGAGGTCT GGCCACGGGG CCTGATCACC 
TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG 
GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG 
CTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG 
CTGTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC TGTCTTCTTC 
TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG 
GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC 
TTCGCCAAGG TCACTGTCTG CTTCCCGCCA GACCCTGCTG CCCCAGCCCC CACCTGGAAG 
CCTGCCGGGC TCCCGCCACC CGCCTTCCGC CGCTCCGAGC CCTTCTTGGC ACCCTTGCTG 
CTGACCGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGGG GCTGCTGCCC ACCGGCCATC 
CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC 
ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGCG GGCCCCACGC ACCCAACGGC 
GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAATA CTGCGGCGGC 
GGCGGGGGCG CTGGGGGCAG TGGGCATGGG GCTGGTGCGG GCCGGAAGGG GCTGTTGAGC 
CAGGACAACC TCACCAGCAT GTCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834866.1
CDS	767..9136
Translation	MALGRALAVALAVSLAVLGPLSPGAQAGDCKGQRQVLREAPGFVTDGAGNYSVNGNCEWLIEAPSSQHRILLDFLFLDTECTYDYLFVYDGDSPRGPLLASLSGSTRPPPIEASSGKMLLHLFSDANYNLLGFNASFRFSLCPGGCRSHGQCQPPGVCVCEPGWGGPDCSLQECSAYCGSHGTCTSPLGPCRCEPGFLGRACDLHLWENQGAGWWHNVSAGDPAFSARIGAAGAFLSPLGLLAVFGGQDLNRALGDLVLYNFSANTWERWDLSPAPAARHSHVAVAWAGSLVLMGGELADGTLTSDIWAFSPMGGGRWELLAPPASSPSGPPGLAGHAAALVDNTWLYVSGGRTQHDLFSSGLFRFRLDGTSGGYWEQVMPAGGRPPAATGHSMVFHAASRALLVHGGHRPSTARFSVRVNSTELFHVDRRVWTTLKGREGLHGPRERAFHTASVLGNYMLVYGGNVHTHYQEEKCYEDGIFFYHLGCHQWVSGAELAPPGTPEGRAAPPSGRYSHVAAVLGGSVLLVAGGYSGRPRGDLMAYKVPPFVFQAPAPDYHLDYCSMYTDHSVCSRDPECSWCQGACQAAPPPGTPSGACPAASCLGLSRLLGDCQACLAFSSSAAPPQGPGALGWCVHNESCLPRPEQARCRGEQISGTVGWWGPAPVFVTSLEACVTQSFLPGLHLLTFQQPPNASQPDKVLIVRSTTITLTPSSETDVSLVYRGFIHPLLPGGPGGSGAEDVAVWARAQRLHVLARMARGPDTENMEEVGRWVAQQEKETRRLQRPGSARLFPLPGRGHKYAVEIRGQLNGSAGPGHSELTLLWDRTGVPGGSEISFFFLEPYRSSACASYSSCLGCLADQGCGWCLASATCHLRQSGAHCRDAGAGGSLLVLVPALCPLCEEHRDCHACTQDPFCEWHQSTSRKGDAACSRRGRGRGALKSPEECPPLCSQRLTCDDCLANSSQCAWCQSTRTCFLFAAYLARYPHGGCRGWDDSVHSEPRCRSCDRFPSCHECLQSHECGWCGNEDNPTLGRCLQGDFSGPLSGGNCSLWVGEGLGLSVALPARWAYARCPDVDECRLGLARCHLRATCLNTPLSYECHCQRGYQGDGVTYCNRTCLEDCGHGVCSGPPDFTCVCDLGWTSDLPLPTPAPGPPAPRCSRDCGCNFHSHCHKRGPGFCDECQDWTWGEHCERCRPGSFGNATGSGGCRPCQCNGHGDPRRGHCDNLSGLCFCQDHTEGAHCQLCSPGYYGDPRAGGSCFRECGGRALLTNVSSVALGSRRFGGLLPAGGGAARAGPGLSYCVWVVSATEVLQPCAPGTLCPPLTLTFSPDSSTPCTLSYVLAFDGFPRFLDTGVVQSDRSLIAAFCGQRRDRPLTVQALSGLLVLHWEANGSSSWGFNASVGSARCGSGGPGSCPVPQECVPQDGVPGAGLCRCPQGWAGPHCRMAVCPENCNAHNGAGTCNQSLGVCVCAEGFGGPDCATKLDGGQLVWETLMDSRLSADTASRFLHRLGHTMVEGPDATLWMFGGLGLPQGLLGNLYRYSVSERRWTQMLAGAEDGGPGPSPRSFHAAAYVPAGRGAMYLLGGLTAGGIARDFWVLNLTTLQWRQEKAPQTVELPAVAGHTLTARRGLSLLLVGGYSPENGFNQQLLEYQLATGTWVSGAQSGTPPTGLYGHSAVYHEATDSLYVFGGFRFHVELAAPSPELYSLHCPDRTWSLLAPSQGAKPRPRLFHASALLGDTMVVLGGRSDPDEFSSDVLLYQVNCNTWLLPDLIGPASVGPPMEESVAHAVAAVGGHLFISGGFGGVALGRLLALTLPPDPCRLLPSPEACNQTGACTWCHGACLSGDQAHRLGCGGTPCSPMPHSPEECRRLRTCSECLARHPRTLQPGDGEASAPRCKWCTNCPEGACIGRNGSCTSENDCRINQREVFWAGNCSEAVCGAADCEQCTREGKCMWTRQFKRTGETRRILSVQPTYDWTCFSHSLLNVSPMPVESSPPLPCPTPCHLLPNCTSCLDSKGADGGWQHCVWSSSLQQCLSPSYLPLRCMAGGCGRLLRGPESCSLGCAQATQCALCLRRPHCGWCTWGGQDGGGRCLEGGLSGPRDGLTCGRPGASWAFLSCPPEDECANGHHDCNETQNCHDRPHGYECSCKAGYTMDNVTGLCRPVCAQGCVNGSCVEPDHCRCHFGFVGRNCSTECRCNRHSDCAGVGARDHCLLCHNHTKGSHCEQCLPLFVGSAVGGGTCRPCHAFCRGNSHVCVSRKELEIARREPEKYSLDPDEIEHWATEGPSEDEAVCVNCQNNSYGDKCESCLHGYFLLDGKCTKCQCNGHADTCNEQDGTGCPCQNNTETGTCQGSSPSDRRDCYKYQCAKCRESFHGSPLGGQQCYRLISVEQEYCLDPTSQTNCFHEPKRRALGPGRTVLFGVQPKFTNVDIRLTLDVTFGAVDLYVSTSYDTFVVRVAPDTGVHTVHIQPPPPPPPPPPPADGGPRGPGDLGGPGAGGGPATPAEPRVREVWPRGLITYVTVTEPSAVLVVRGVRDRLVITYPHEHHTLKSSRFYLLLLGVGDPSGPSANGSADSQGLLFFRQDQAHIDLFVFFSVFFSCFFLFLSLCVLLWKAKQALDHRQEQRRHLQEMTKMASRPFAKVTVCFPPDPAAPAPTWKPAGLPPPAFRRSEPFLAPLLLTGAGGPWGPVGGGCCPPAIPTTTAGLRAGPITLEPTEDGMAGVATLLLQLPGGPHAPNGACLGSALVTLRHRLHEYCGGGGGAGGSGHGAGAGRKGLLSQDNLTSMSL

Target ORF information:

RefSeq Version	XM_024979098.1
Organism	Bos taurus(cattle)
Definition	Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979098.1

ORF Insert Sequence:

ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTGTAT CATTGGCCGT GCTGGGGCCA 
CTGTCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT 
CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC 
ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA 
TGCACTTATG ACTACCTGTT CGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC 
AGTCTGAGTG GGAGCACACG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTG 
CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC 
CTGTGCCCGG GTGGCTGCCG CAGCCATGGG CAGTGCCAGC CCCCAGGGGT GTGTGTCTGT 
GAGCCGGGCT GGGGGGGCCC CGACTGCAGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC 
CATGGCACCT GCACCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGC 
GCCTGTGACC TGCACCTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC 
GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACTGGGG 
CTGCTGGCTG TTTTTGGAGG CCAGGACCTC AACCGCGCGC TGGGGGACCT CGTCCTGTAC 
AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC 
TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT 
GGCACCCTCA CCAGCGACAT CTGGGCCTTT AGCCCGATGG GTGGCGGCCG CTGGGAGCTT 
CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA TGCAGCAGCC 
CTGGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC 
TCCGGCCTCT TCCGTTTCCG CCTCGACGGC ACCAGTGGGG GCTATTGGGA GCAGGTGATG 
CCAGCAGGTG GGCGGCCCCC TGCCGCCACC GGCCATTCCA TGGTGTTCCA TGCCGCCTCC 
CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG 
AACTCCACCG AGCTCTTCCA TGTGGATCGG CGCGTGTGGA CCACCCTGAA GGGCCGGGAG 
GGACTTCACG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG 
CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC 
ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCTGAGCT TGCCCCTCCA 
GGAACTCCAG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG 
CTTGGTGGCA GTGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCTCG TGGGGACTTG 
ATGGCCTACA AAGTGCCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGATTA CCACTTGGAC 
TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGTTGGTGT 
CAGGGGGCCT GCCAAGCCGC CCCACCTCCT GGGACTCCCT CAGGGGCTTG TCCGGCTGCC 
AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC 
AGTGCGGCTC CCCCCCAGGG GCCCGGTGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC 
CTCCCTCGGC CTGAGCAGGC CCGCTGCCGC GGGGAGCAGA TCTCCGGCAC CGTGGGCTGG 
TGGGGGCCCG CTCCTGTCTT TGTGACCTCC CTGGAGGCCT GTGTCACCCA GAGCTTCCTG 
CCTGGCCTGC ACCTGCTCAC CTTCCAGCAG CCACCCAACG CCTCCCAGCC TGACAAGGTC 
TTGATCGTCC GCAGCACGAC CATCACCCTG ACGCCGAGCT CAGAGACCGA CGTGTCCCTG 
GTCTACCGTG GCTTCATCCA CCCGCTGCTG CCGGGAGGCC CTGGGGGGTC TGGGGCTGAG 
GATGTGGCCG TGTGGGCCCG GGCCCAGCGC CTGCATGTGC TGGCCCGGAT GGCCCGGGGC 
CCGGACACAG AAAACATGGA GGAGGTGGGG CGCTGGGTGG CACAGCAGGA GAAGGAGACG 
AGGCGGCTGC AGCGCCCAGG GTCAGCTCGC CTCTTCCCAC TGCCCGGCCG GGGCCACAAG 
TATGCAGTAG AGATCCGGGG CCAGCTCAAT GGCTCAGCAG GCCCCGGGCA CAGTGAGCTC 
ACCCTGCTGT GGGACCGGAC TGGCGTGCCA GGTGGCAGCG AGATCTCCTT CTTCTTCCTG 
GAGCCCTACC GCTCGTCGGC CTGCGCCTCC TACTCCTCCT GCCTGGGCTG CCTGGCAGAC 
CAGGGCTGCG GCTGGTGCCT GGCCAGCGCC ACCTGCCACC TGCGCCAGAG CGGCGCCCAC 
TGTAGGGACG CTGGGGCCGG CGGGTCCCTG CTCGTGCTGG TGCCTGCCCT CTGCCCGCTC 
TGCGAGGAGC ATCGCGACTG CCACGCCTGC ACCCAGGACC CCTTCTGTGA GTGGCATCAG 
AGCACCAGCC GCAAGGGGGA CGCGGCGTGC AGCCGGCGCG GCCGGGGTCG GGGCGCCCTG 
AAGAGTCCGG AAGAGTGCCC CCCACTCTGC AGCCAGCGCC TGACCTGTGA CGACTGCCTG 
GCCAACTCGA GCCAGTGCGC CTGGTGCCAG TCCACCCGCA CCTGCTTCCT GTTCGCCGCC 
TACCTGGCCC GGTACCCGCA CGGGGGCTGC CGAGGCTGGG ACGACAGCGT GCACTCGGAG 
CCTCGGTGCC GGAGCTGTGA CCGCTTCCCG AGCTGTCACG AGTGTCTGCA GAGCCATGAG 
TGTGGCTGGT GTGGAAACGA GGACAACCCC ACGCTGGGCC GTTGCCTCCA AGGGGACTTC 
TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT 
GTGGCCCTCC CCGCCCGCTG GGCGTATGCC CGCTGTCCGG ATGTAGACGA GTGCCGCCTG 
GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTACGAGTGT 
CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG 
GACTGTGGCC ACGGTGTGTG CAGCGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC 
TGGACATCAG ACCTGCCCCT TCCCACGCCG GCCCCCGGCC CCCCCGCCCC CCGCTGTTCG 
CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAT 
GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCTGG AAGCTTCGGG 
AATGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ACGGGCATGG GGACCCTCGC 
CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC 
CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC 
CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC 
CGGTTTGGGG GGCTCCTGCC TGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC 
TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTGCAGC CCTGTGCCCC CGGGACCCTC 
TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAT 
GTCCTGGCCT TTGATGGATT CCCACGCTTC CTAGACACTG GTGTCGTCCA GTCGGACCGT 
AGCCTCATCG CTGCGTTTTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG 
TCTGGGCTGC TGGTACTGCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAATGCC 
TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCCGGGA GCTGCCCTGT CCCCCAGGAG 
TGTGTGCCCC AGGATGGGGT CCCAGGTGCT GGACTCTGCC GGTGTCCCCA GGGCTGGGCT 
GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG 
ACGTGTAATC AGAGCCTGGG CGTGTGCGTC TGTGCTGAGG GCTTTGGGGG TCCTGACTGT 
GCCACGAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA 
GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT 
GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT 
AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCCGA GGATGGGGGC 
CCAGGCCCCT CCCCTCGCTC TTTCCACGCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC 
ATGTACCTGC TGGGGGGGCT CACAGCTGGG GGCATCGCCC GGGACTTCTG GGTCCTCAAC 
CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCCGTT 
GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC 
CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG 
TCCGGGGCTC AGAGCGGGAC ACCCCCCACA GGTCTCTACG GTCACTCTGC TGTGTATCAT 
GAGGCCACCG ACTCCCTCTA CGTGTTCGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC 
CCGTCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT 
TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCACGCCT CGGCCTTGCT AGGGGACACC 
ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGCGACGT GCTGCTCTAC 
CAGGTCAACT GCAACACCTG GCTCCTGCCC GACCTCATCG GCCCAGCCTC TGTGGGACCG 
CCAATGGAAG AGTCTGTGGC TCACGCCGTG GCCGCGGTAG GGGGTCACCT GTTTATCTCA 
GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCC TGACCCTGCC CCCTGACCCC 
TGCCGCCTGC TGCCCTCGCC CGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT 
GGAGCCTGCC TGTCTGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCAC CCCCTGCTCC 
CCTATGCCCC ACTCCCCCGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC 
CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCT CTGCCCCACG CTGTAAGTGG 
TGTACCAACT GCCCCGAGGG TGCCTGCATT GGGCGCAACG GGTCCTGCAC CTCAGAGAAT 
GACTGTCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCGGA GGCTGTGTGC 
GGGGCGGCCG ACTGCGAGCA GTGCACCAGG GAGGGCAAAT GCATGTGGAC GCGGCAGTTC 
AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC 
TTCAGCCACT CACTGCTGAA CGTGTCCCCG ATGCCAGTGG AGTCATCGCC CCCGCTGCCC 
TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT 
GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC 
TATCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC 
TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGTGCCCTGT GCCTGCGGCG CCCCCACTGT 
GGCTGGTGTA CCTGGGGGGG CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT 
GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CCTGGGCCTT TCTTTCCTGC 
CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ACGAGACCCA GAACTGCCAC 
GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG 
GGGCTGTGCC GCCCAGTGTG CGCCCAGGGT TGCGTGAACG GCTCGTGTGT GGAGCCCGAC 
CACTGCCGCT GCCACTTCGG CTTCGTCGGC CGCAACTGTT CCACGGAGTG TCGCTGCAAC 
CGCCACAGCG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAATCAC 
ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCGCTGTTCG TGGGCTCAGC CGTAGGGGGA 
GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGAAACA GCCACGTCTG CGTCTCCAGG 
AAGGAGCTTG AGATAGCCAG GAGGGAGCCG GAGAAGTACT CGCTGGATCC AGATGAGATT 
GAACACTGGG CGACAGAGGG TCCCAGCGAA GATGAGGCTG TGTGTGTGAA CTGCCAGAAT 
AACAGCTATG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTCCTCCT CGACGGGAAA 
TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACATGTA ATGAGCAGGA TGGGACAGGC 
TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC 
CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA 
GGGGGCCAGC AGTGCTACCG CCTCATCTCC GTGGAACAGG AGTACTGCCT GGACCCCACG 
TCCCAAACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGTCCCGG ACGCACCGTT 
CTCTTTGGAG TGCAGCCTAA GTTCACCAAC GTGGACATCC GCCTGACGCT GGACGTGACC 
TTTGGCGCCG TGGACCTCTA CGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT 
CCCGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CACCTCCCCC ACCCCCACCA 
CCCCCTGCTG ACGGCGGGCC CCGGGGACCT GGGGATCTCG GGGGACCTGG GGCTGGAGGT 
GGGCCGGCTA CCCCAGCAGA GCCGCGAGTG CGGGAGGTCT GGCCACGGGG CCTGATCACC 
TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG 
GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG 
CTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG 
CTGTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC TGTCTTCTTC 
TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG 
GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC 
TTCGCCAAGG TCACTGTCTG CTTCCCGCCA GACCCTGCTG CCCCAGCCCC CACCTGGAAG 
CCTGCCGGGC TCCCGCCACC CGCCTTCCGC CGCTCCGAGC CCTTCTTGGC ACCCTTGCTG 
CTGACCGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGGG GCTGCTGCCC ACCGGCCATC 
CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC 
ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGCG GGCCCCACGC ACCCAACGGC 
GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAATA CTGCGGCGGC 
GGCGGGGGCG CTGGGGGCAG TGGGCATGGG GCTGGTGCGG GCCGGAAGGG GCTGTTGAGC 
CAGGACAACC TCACCAGCAT GTCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF8 ( XM_024979099.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834867.1 Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X2, mRNA.

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CloneID	OBa225576
Clone ID Related Accession (Same CDS sequence)	XM_024979099.1
Accession Version	XM_024979099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	multiple epidermal growth factor-like domains protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTCTTCAG CGATGCCAAC TACAACCTGC TGGGCTTCAA CGCCTCCTTC 
CGCTTCTCCC TGTGCCCGGG TGGCTGCCGC AGCCATGGGC AGTGCCAGCC CCCAGGGGTG 
TGTGTCTGTG AGCCGGGCTG GGGGGGCCCC GACTGCAGCC TGCAGGAGTG CTCAGCCTAC 
TGCGGCAGCC ATGGCACCTG CACCTCGCCC CTGGGACCGT GCCGCTGTGA GCCTGGTTTC 
CTGGGACGCG CCTGTGACCT GCACCTGTGG GAGAACCAGG GGGCTGGCTG GTGGCACAAC 
GTGAGTGCCG GGGACCCCGC CTTCTCTGCC CGTATCGGGG CAGCCGGGGC CTTCCTGTCC 
CCACTGGGGC TGCTGGCTGT TTTTGGAGGC CAGGACCTCA ACCGCGCGCT GGGGGACCTC 
GTCCTGTACA ACTTCTCTGC CAACACCTGG GAGCGCTGGG ACCTGAGCCC TGCCCCGGCT 
GCCCGTCACT CCCACGTGGC TGTGGCTTGG GCCGGCTCCC TGGTGCTGAT GGGCGGTGAG 
CTGGCTGATG GCACCCTCAC CAGCGACATC TGGGCCTTTA GCCCGATGGG TGGCGGCCGC 
TGGGAGCTTC TGGCACCACC TGCCTCCAGT CCCTCGGGGC CGCCCGGCCT GGCAGGTCAT 
GCAGCAGCCC TGGTGGACAA CACGTGGCTC TATGTGTCTG GTGGCCGCAC CCAGCACGAC 
CTCTTCTCCT CCGGCCTCTT CCGTTTCCGC CTCGACGGCA CCAGTGGGGG CTATTGGGAG 
CAGGTGATGC CAGCAGGTGG GCGGCCCCCT GCCGCCACCG GCCATTCCAT GGTGTTCCAT 
GCCGCCTCCC GTGCCCTGCT GGTTCACGGT GGACACCGGC CCTCCACTGC CCGATTCTCT 
GTGCGGGTGA ACTCCACCGA GCTCTTCCAT GTGGATCGGC GCGTGTGGAC CACCCTGAAG 
GGCCGGGAGG GACTTCACGG CCCACGGGAG CGAGCCTTCC ACACGGCCAG CGTCCTGGGC 
AACTACATGC TGGTCTATGG GGGCAACGTG CACACCCACT ACCAGGAGGA AAAGTGCTAT 
GAAGACGGCA TCTTCTTCTA CCACCTGGGC TGCCATCAGT GGGTGTCAGG GGCTGAGCTT 
GCCCCTCCAG GAACTCCAGA GGGCCGAGCA GCGCCTCCTA GTGGCCGGTA CTCACACGTG 
GCGGCTGTGC TTGGTGGCAG TGTCCTGTTG GTGGCCGGGG GGTACAGTGG CCGGCCTCGT 
GGGGACTTGA TGGCCTACAA AGTGCCCCCC TTTGTGTTCC AGGCGCCTGC TCCAGATTAC 
CACTTGGACT ATTGCTCCAT GTACACGGAC CACAGCGTCT GCTCCCGAGA CCCCGAATGC 
AGTTGGTGTC AGGGGGCCTG CCAAGCCGCC CCACCTCCTG GGACTCCCTC AGGGGCTTGT 
CCGGCTGCCA GCTGCCTGGG CCTGAGCCGT CTCCTGGGTG ACTGCCAGGC CTGCCTGGCG 
TTCAGCAGCA GTGCGGCTCC CCCCCAGGGG CCCGGTGCCC TGGGCTGGTG CGTGCACAAT 
GAGAGCTGCC TCCCTCGGCC TGAGCAGGCC CGCTGCCGCG GGGAGCAGAT CTCCGGCACC 
GTGGGCTGGT GGGGGCCCGC TCCTGTCTTT GTGACCTCCC TGGAGGCCTG TGTCACCCAG 
AGCTTCCTGC CTGGCCTGCA CCTGCTCACC TTCCAGCAGC CACCCAACGC CTCCCAGCCT 
GACAAGGTCT TGATCGTCCG CAGCACGACC ATCACCCTGA CGCCGAGCTC AGAGACCGAC 
GTGTCCCTGG TCTACCGTGG CTTCATCCAC CCGCTGCTGC CGGGAGGCCC TGGGGGGTCT 
GGGGCTGAGG ATGTGGCCGT GTGGGCCCGG GCCCAGCGCC TGCATGTGCT GGCCCGGATG 
GCCCGGGGCC CGGACACAGA AAACATGGAG GAGGTGGGGC GCTGGGTGGC ACAGCAGGAG 
AAGGAGACGA GGCGGCTGCA GCGCCCAGGG TCAGCTCGCC TCTTCCCACT GCCCGGCCGG 
GGCCACAAGT ATGCAGTAGA GATCCGGGGC CAGCTCAATG GCTCAGCAGG CCCCGGGCAC 
AGTGAGCTCA CCCTGCTGTG GGACCGGACT GGCGTGCCAG GTGGCAGCGA GATCTCCTTC 
TTCTTCCTGG AGCCCTACCG CTCGTCGGCC TGCGCCTCCT ACTCCTCCTG CCTGGGCTGC 
CTGGCAGACC AGGGCTGCGG CTGGTGCCTG GCCAGCGCCA CCTGCCACCT GCGCCAGAGC 
GGCGCCCACT GTAGGGACGC TGGGGCCGGC GGGTCCCTGC TCGTGCTGGT GCCTGCCCTC 
TGCCCGCTCT GCGAGGAGCA TCGCGACTGC CACGCCTGCA CCCAGGACCC CTTCTGTGAG 
TGGCATCAGA GCACCAGCCG CAAGGGGGAC GCGGCGTGCA GCCGGCGCGG CCGGGGTCGG 
GGCGCCCTGA AGAGTCCGGA AGAGTGCCCC CCACTCTGCA GCCAGCGCCT GACCTGTGAC 
GACTGCCTGG CCAACTCGAG CCAGTGCGCC TGGTGCCAGT CCACCCGCAC CTGCTTCCTG 
TTCGCCGCCT ACCTGGCCCG GTACCCGCAC GGGGGCTGCC GAGGCTGGGA CGACAGCGTG 
CACTCGGAGC CTCGGTGCCG GAGCTGTGAC CGCTTCCCGA GCTGTCACGA GTGTCTGCAG 
AGCCATGAGT GTGGCTGGTG TGGAAACGAG GACAACCCCA CGCTGGGCCG TTGCCTCCAA 
GGGGACTTCT CAGGGCCTCT GAGTGGGGGG AACTGCTCCC TGTGGGTCGG GGAGGGCCTG 
GGGCTGTCTG TGGCCCTCCC CGCCCGCTGG GCGTATGCCC GCTGTCCGGA TGTAGACGAG 
TGCCGCCTGG GCCTGGCGCG GTGCCACCTG CGGGCGACCT GCCTGAACAC ACCCCTCAGC 
TACGAGTGTC ACTGCCAGAG GGGCTACCAG GGCGATGGCG TCACTTACTG CAACCGCACG 
TGCCTGGAGG ACTGTGGCCA CGGTGTGTGC AGCGGCCCCC CAGACTTCAC CTGCGTGTGT 
GACCTGGGCT GGACATCAGA CCTGCCCCTT CCCACGCCGG CCCCCGGCCC CCCCGCCCCC 
CGCTGTTCGC GGGACTGTGG CTGCAACTTC CACAGCCACT GCCACAAGCG GGGGCCCGGC 
TTCTGCGATG AGTGCCAGGA CTGGACGTGG GGGGAGCACT GTGAGCGGTG CCGGCCTGGA 
AGCTTCGGGA ATGCCACAGG CTCCGGCGGC TGCCGGCCCT GCCAGTGCAA CGGGCATGGG 
GACCCTCGCC GAGGCCACTG TGACAACCTC AGCGGGCTCT GCTTCTGCCA GGACCACACC 
GAGGGTGCCC ACTGCCAGCT CTGCTCCCCT GGCTACTACG GGGACCCTCG GGCAGGTGGC 
TCCTGCTTCC GGGAGTGTGG GGGTCGTGCC CTCCTCACCA ACGTGTCCTC AGTGGCACTG 
GGCTCACGCC GGTTTGGGGG GCTCCTGCCT GCAGGTGGCG GGGCAGCAAG AGCGGGGCCT 
GGCCTGTCCT ACTGTGTGTG GGTTGTCTCG GCCACTGAGG TGCTGCAGCC CTGTGCCCCC 
GGGACCCTCT GTCCCCCGCT TACCCTCACC TTCTCCCCCG ACAGCAGCAC CCCGTGCACG 
CTGAGCTATG TCCTGGCCTT TGATGGATTC CCACGCTTCC TAGACACTGG TGTCGTCCAG 
TCGGACCGTA GCCTCATCGC TGCGTTTTGC GGCCAGCGGC GGGACCGGCC CCTCACTGTG 
CAGGCCCTGT CTGGGCTGCT GGTACTGCAC TGGGAGGCCA ATGGCTCCTC ATCCTGGGGC 
TTCAATGCCT CCGTGGGCTC TGCCCGCTGT GGGTCAGGGG GCCCCGGGAG CTGCCCTGTC 
CCCCAGGAGT GTGTGCCCCA GGATGGGGTC CCAGGTGCTG GACTCTGCCG GTGTCCCCAG 
GGCTGGGCTG GCCCGCACTG CCGCATGGCT GTGTGTCCTG AGAACTGTAA TGCCCACAAC 
GGGGCGGGGA CGTGTAATCA GAGCCTGGGC GTGTGCGTCT GTGCTGAGGG CTTTGGGGGT 
CCTGACTGTG CCACGAAGCT GGATGGTGGG CAGCTGGTCT GGGAGACCCT CATGGACAGC 
CGCCTCTCAG CTGACACTGC CAGCCGCTTC CTGCACCGCC TGGGGCACAC GATGGTGGAG 
GGACCTGATG CCACCTTGTG GATGTTTGGG GGCCTGGGCC TGCCCCAGGG GCTGCTGGGA 
AACCTGTATA GGTACTCAGT GAGTGAACGG CGGTGGACAC AGATGTTGGC AGGAGCCGAG 
GATGGGGGCC CAGGCCCCTC CCCTCGCTCT TTCCACGCTG CTGCCTACGT GCCTGCTGGC 
CGTGGGGCCA TGTACCTGCT GGGGGGGCTC ACAGCTGGGG GCATCGCCCG GGACTTCTGG 
GTCCTCAACC TCACCACCCT GCAGTGGCGG CAGGAGAAGG CCCCTCAGAC TGTGGAGCTG 
CCAGCCGTTG CTGGTCACAC GCTTACTGCC CGCCGAGGCC TATCTCTGCT CCTGGTGGGT 
GGTTACTCCC CGGAAAATGG CTTCAACCAG CAGCTGCTTG AGTACCAGCT GGCGACTGGC 
ACCTGGGTGT CCGGGGCTCA GAGCGGGACA CCCCCCACAG GTCTCTACGG TCACTCTGCT 
GTGTATCATG AGGCCACCGA CTCCCTCTAC GTGTTCGGGG GTTTCCGCTT CCACGTGGAG 
CTGGCAGCCC CGTCCCCTGA GCTCTACTCC CTGCACTGCC CTGACCGCAC CTGGAGCCTG 
CTGGCCCCTT CTCAGGGAGC AAAGCCCCGG CCCCGACTCT TCCACGCCTC GGCCTTGCTA 
GGGGACACCA TGGTGGTCCT CGGGGGGCGC TCAGACCCCG ACGAGTTCAG CAGCGACGTG 
CTGCTCTACC AGGTCAACTG CAACACCTGG CTCCTGCCCG ACCTCATCGG CCCAGCCTCT 
GTGGGACCGC CAATGGAAGA GTCTGTGGCT CACGCCGTGG CCGCGGTAGG GGGTCACCTG 
TTTATCTCAG GAGGCTTTGG GGGAGTGGCC CTGGGCCGCC TGCTGGCCCT GACCCTGCCC 
CCTGACCCCT GCCGCCTGCT GCCCTCGCCC GAAGCTTGTA ACCAGACTGG GGCCTGTACC 
TGGTGCCATG GAGCCTGCCT GTCTGGGGAC CAGGCCCACA GGCTGGGCTG TGGCGGCACC 
CCCTGCTCCC CTATGCCCCA CTCCCCCGAG GAATGTCGAC GTCTGCGGAC CTGCAGCGAG 
TGCCTGGCCC GCCATCCCCG GACCCTGCAG CCTGGAGATG GAGAGGCCTC TGCCCCACGC 
TGTAAGTGGT GTACCAACTG CCCCGAGGGT GCCTGCATTG GGCGCAACGG GTCCTGCACC 
TCAGAGAATG ACTGTCGCAT CAACCAGCGC GAGGTCTTCT GGGCCGGGAA CTGCTCGGAG 
GCTGTGTGCG GGGCGGCCGA CTGCGAGCAG TGCACCAGGG AGGGCAAATG CATGTGGACG 
CGGCAGTTCA AGAGGACGGG GGAGACCCGC CGCATCCTTT CGGTGCAGCC CACCTACGAC 
TGGACGTGCT TCAGCCACTC ACTGCTGAAC GTGTCCCCGA TGCCAGTGGA GTCATCGCCC 
CCGCTGCCCT GCCCCACACC CTGTCACCTC CTGCCCAACT GCACCTCCTG CCTGGACTCC 
AAGGGTGCTG ACGGGGGCTG GCAGCACTGC GTCTGGAGCA GCAGCCTCCA GCAGTGTCTG 
AGCCCCTCCT ATCTGCCCCT GCGGTGTATG GCCGGAGGCT GTGGGCGGCT GCTCCGCGGA 
CCCGAGAGCT GCTCCCTGGG CTGTGCTCAG GCAACCCAGT GTGCCCTGTG CCTGCGGCGC 
CCCCACTGTG GCTGGTGTAC CTGGGGGGGC CAGGATGGGG GCGGCCGCTG CCTGGAGGGT 
GGACTCAGTG GCCCCCGTGA CGGGCTGACG TGTGGGCGAC CTGGGGCCTC CTGGGCCTTT 
CTTTCCTGCC CCCCGGAGGA CGAATGTGCC AACGGGCACC ATGACTGCAA CGAGACCCAG 
AACTGCCACG ACCGGCCCCA CGGTTACGAG TGCAGCTGCA AGGCAGGCTA CACCATGGAC 
AACGTGACGG GGCTGTGCCG CCCAGTGTGC GCCCAGGGTT GCGTGAACGG CTCGTGTGTG 
GAGCCCGACC ACTGCCGCTG CCACTTCGGC TTCGTCGGCC GCAACTGTTC CACGGAGTGT 
CGCTGCAACC GCCACAGCGA CTGCGCCGGC GTGGGGGCCC GCGACCACTG CCTGCTCTGC 
CACAATCACA CCAAGGGCAG CCACTGTGAG CAGTGCCTCC CGCTGTTCGT GGGCTCAGCC 
GTAGGGGGAG GGACCTGCCG GCCCTGCCAC GCCTTTTGTC GCGGAAACAG CCACGTCTGC 
GTCTCCAGGA AGGAGCTTGA GATAGCCAGG AGGGAGCCGG AGAAGTACTC GCTGGATCCA 
GATGAGATTG AACACTGGGC GACAGAGGGT CCCAGCGAAG ATGAGGCTGT GTGTGTGAAC 
TGCCAGAATA ACAGCTATGG GGATAAGTGT GAGAGCTGCC TCCACGGCTA CTTCCTCCTC 
GACGGGAAAT GCACCAAATG CCAGTGTAAT GGCCACGCGG ACACATGTAA TGAGCAGGAT 
GGGACAGGCT GCCCATGTCA GAACAACACA GAGACAGGCA CGTGCCAGGG CAGCTCCCCC 
AGTGACCGCC GAGACTGCTA CAAATACCAG TGTGCCAAGT GCCGAGAGTC ATTCCACGGG 
AGCCCGCTAG GGGGCCAGCA GTGCTACCGC CTCATCTCCG TGGAACAGGA GTACTGCCTG 
GACCCCACGT CCCAAACCAA CTGTTTCCAC GAGCCCAAGC GCCGGGCTCT GGGTCCCGGA 
CGCACCGTTC TCTTTGGAGT GCAGCCTAAG TTCACCAACG TGGACATCCG CCTGACGCTG 
GACGTGACCT TTGGCGCCGT GGACCTCTAC GTCTCCACCT CCTATGACAC CTTCGTGGTC 
CGCGTGGCTC CCGACACTGG TGTCCACACC GTGCATATCC AGCCGCCACC ACCTCCCCCA 
CCCCCACCAC CCCCTGCTGA CGGCGGGCCC CGGGGACCTG GGGATCTCGG GGGACCTGGG 
GCTGGAGGTG GGCCGGCTAC CCCAGCAGAG CCGCGAGTGC GGGAGGTCTG GCCACGGGGC 
CTGATCACCT ACGTGACAGT GACAGAGCCG TCGGCGGTGC TGGTGGTCCG TGGCGTGAGG 
GACCGGCTGG TCATCACCTA CCCGCACGAG CACCACACCC TCAAGTCCAG CCGCTTCTAC 
CTGCTCCTGC TGGGTGTGGG AGACCCAAGT GGGCCCAGTG CCAACGGCTC AGCTGACTCG 
CAGGGCTTGC TGTTCTTCCG GCAGGACCAG GCCCACATTG ACCTGTTTGT CTTCTTCTCT 
GTCTTCTTCT CCTGCTTCTT CCTCTTCCTC TCGCTCTGTG TGCTGCTCTG GAAGGCCAAG 
CAGGCCCTGG ACCACCGGCA GGAACAGCGC CGGCACCTGC AGGAGATGAC CAAGATGGCC 
AGCCGCCCCT TCGCCAAGGT CACTGTCTGC TTCCCGCCAG ACCCTGCTGC CCCAGCCCCC 
ACCTGGAAGC CTGCCGGGCT CCCGCCACCC GCCTTCCGCC GCTCCGAGCC CTTCTTGGCA 
CCCTTGCTGC TGACCGGGGC CGGCGGGCCC TGGGGACCTG TAGGAGGGGG CTGCTGCCCA 
CCGGCCATCC CCACCACCAC TGCCGGCCTG CGAGCCGGGC CTATCACCCT TGAGCCCACC 
GAGGATGGCA TGGCCGGCGT GGCCACACTG CTGCTCCAAC TGCCTGGCGG GCCCCACGCA 
CCCAACGGCG CCTGCCTGGG GTCAGCGCTC GTCACACTGC GGCACCGGCT GCACGAATAC 
TGCGGCGGCG GCGGGGGCGC TGGGGGCAGT GGGCATGGGG CTGGTGCGGG CCGGAAGGGG 
CTGTTGAGCC AGGACAACCT CACCAGCATG TCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834867.1
CDS	473..8491
Translation	MLLHLFSDANYNLLGFNASFRFSLCPGGCRSHGQCQPPGVCVCEPGWGGPDCSLQECSAYCGSHGTCTSPLGPCRCEPGFLGRACDLHLWENQGAGWWHNVSAGDPAFSARIGAAGAFLSPLGLLAVFGGQDLNRALGDLVLYNFSANTWERWDLSPAPAARHSHVAVAWAGSLVLMGGELADGTLTSDIWAFSPMGGGRWELLAPPASSPSGPPGLAGHAAALVDNTWLYVSGGRTQHDLFSSGLFRFRLDGTSGGYWEQVMPAGGRPPAATGHSMVFHAASRALLVHGGHRPSTARFSVRVNSTELFHVDRRVWTTLKGREGLHGPRERAFHTASVLGNYMLVYGGNVHTHYQEEKCYEDGIFFYHLGCHQWVSGAELAPPGTPEGRAAPPSGRYSHVAAVLGGSVLLVAGGYSGRPRGDLMAYKVPPFVFQAPAPDYHLDYCSMYTDHSVCSRDPECSWCQGACQAAPPPGTPSGACPAASCLGLSRLLGDCQACLAFSSSAAPPQGPGALGWCVHNESCLPRPEQARCRGEQISGTVGWWGPAPVFVTSLEACVTQSFLPGLHLLTFQQPPNASQPDKVLIVRSTTITLTPSSETDVSLVYRGFIHPLLPGGPGGSGAEDVAVWARAQRLHVLARMARGPDTENMEEVGRWVAQQEKETRRLQRPGSARLFPLPGRGHKYAVEIRGQLNGSAGPGHSELTLLWDRTGVPGGSEISFFFLEPYRSSACASYSSCLGCLADQGCGWCLASATCHLRQSGAHCRDAGAGGSLLVLVPALCPLCEEHRDCHACTQDPFCEWHQSTSRKGDAACSRRGRGRGALKSPEECPPLCSQRLTCDDCLANSSQCAWCQSTRTCFLFAAYLARYPHGGCRGWDDSVHSEPRCRSCDRFPSCHECLQSHECGWCGNEDNPTLGRCLQGDFSGPLSGGNCSLWVGEGLGLSVALPARWAYARCPDVDECRLGLARCHLRATCLNTPLSYECHCQRGYQGDGVTYCNRTCLEDCGHGVCSGPPDFTCVCDLGWTSDLPLPTPAPGPPAPRCSRDCGCNFHSHCHKRGPGFCDECQDWTWGEHCERCRPGSFGNATGSGGCRPCQCNGHGDPRRGHCDNLSGLCFCQDHTEGAHCQLCSPGYYGDPRAGGSCFRECGGRALLTNVSSVALGSRRFGGLLPAGGGAARAGPGLSYCVWVVSATEVLQPCAPGTLCPPLTLTFSPDSSTPCTLSYVLAFDGFPRFLDTGVVQSDRSLIAAFCGQRRDRPLTVQALSGLLVLHWEANGSSSWGFNASVGSARCGSGGPGSCPVPQECVPQDGVPGAGLCRCPQGWAGPHCRMAVCPENCNAHNGAGTCNQSLGVCVCAEGFGGPDCATKLDGGQLVWETLMDSRLSADTASRFLHRLGHTMVEGPDATLWMFGGLGLPQGLLGNLYRYSVSERRWTQMLAGAEDGGPGPSPRSFHAAAYVPAGRGAMYLLGGLTAGGIARDFWVLNLTTLQWRQEKAPQTVELPAVAGHTLTARRGLSLLLVGGYSPENGFNQQLLEYQLATGTWVSGAQSGTPPTGLYGHSAVYHEATDSLYVFGGFRFHVELAAPSPELYSLHCPDRTWSLLAPSQGAKPRPRLFHASALLGDTMVVLGGRSDPDEFSSDVLLYQVNCNTWLLPDLIGPASVGPPMEESVAHAVAAVGGHLFISGGFGGVALGRLLALTLPPDPCRLLPSPEACNQTGACTWCHGACLSGDQAHRLGCGGTPCSPMPHSPEECRRLRTCSECLARHPRTLQPGDGEASAPRCKWCTNCPEGACIGRNGSCTSENDCRINQREVFWAGNCSEAVCGAADCEQCTREGKCMWTRQFKRTGETRRILSVQPTYDWTCFSHSLLNVSPMPVESSPPLPCPTPCHLLPNCTSCLDSKGADGGWQHCVWSSSLQQCLSPSYLPLRCMAGGCGRLLRGPESCSLGCAQATQCALCLRRPHCGWCTWGGQDGGGRCLEGGLSGPRDGLTCGRPGASWAFLSCPPEDECANGHHDCNETQNCHDRPHGYECSCKAGYTMDNVTGLCRPVCAQGCVNGSCVEPDHCRCHFGFVGRNCSTECRCNRHSDCAGVGARDHCLLCHNHTKGSHCEQCLPLFVGSAVGGGTCRPCHAFCRGNSHVCVSRKELEIARREPEKYSLDPDEIEHWATEGPSEDEAVCVNCQNNSYGDKCESCLHGYFLLDGKCTKCQCNGHADTCNEQDGTGCPCQNNTETGTCQGSSPSDRRDCYKYQCAKCRESFHGSPLGGQQCYRLISVEQEYCLDPTSQTNCFHEPKRRALGPGRTVLFGVQPKFTNVDIRLTLDVTFGAVDLYVSTSYDTFVVRVAPDTGVHTVHIQPPPPPPPPPPPADGGPRGPGDLGGPGAGGGPATPAEPRVREVWPRGLITYVTVTEPSAVLVVRGVRDRLVITYPHEHHTLKSSRFYLLLLGVGDPSGPSANGSADSQGLLFFRQDQAHIDLFVFFSVFFSCFFLFLSLCVLLWKAKQALDHRQEQRRHLQEMTKMASRPFAKVTVCFPPDPAAPAPTWKPAGLPPPAFRRSEPFLAPLLLTGAGGPWGPVGGGCCPPAIPTTTAGLRAGPITLEPTEDGMAGVATLLLQLPGGPHAPNGACLGSALVTLRHRLHEYCGGGGGAGGSGHGAGAGRKGLLSQDNLTSMSL

Target ORF information:

RefSeq Version	XM_024979099.1
Organism	Bos taurus(cattle)
Definition	Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979099.1

ORF Insert Sequence:

ATGCTGCTGC ACCTCTTCAG CGATGCCAAC TACAACCTGC TGGGCTTCAA CGCCTCCTTC 
CGCTTCTCCC TGTGCCCGGG TGGCTGCCGC AGCCATGGGC AGTGCCAGCC CCCAGGGGTG 
TGTGTCTGTG AGCCGGGCTG GGGGGGCCCC GACTGCAGCC TGCAGGAGTG CTCAGCCTAC 
TGCGGCAGCC ATGGCACCTG CACCTCGCCC CTGGGACCGT GCCGCTGTGA GCCTGGTTTC 
CTGGGACGCG CCTGTGACCT GCACCTGTGG GAGAACCAGG GGGCTGGCTG GTGGCACAAC 
GTGAGTGCCG GGGACCCCGC CTTCTCTGCC CGTATCGGGG CAGCCGGGGC CTTCCTGTCC 
CCACTGGGGC TGCTGGCTGT TTTTGGAGGC CAGGACCTCA ACCGCGCGCT GGGGGACCTC 
GTCCTGTACA ACTTCTCTGC CAACACCTGG GAGCGCTGGG ACCTGAGCCC TGCCCCGGCT 
GCCCGTCACT CCCACGTGGC TGTGGCTTGG GCCGGCTCCC TGGTGCTGAT GGGCGGTGAG 
CTGGCTGATG GCACCCTCAC CAGCGACATC TGGGCCTTTA GCCCGATGGG TGGCGGCCGC 
TGGGAGCTTC TGGCACCACC TGCCTCCAGT CCCTCGGGGC CGCCCGGCCT GGCAGGTCAT 
GCAGCAGCCC TGGTGGACAA CACGTGGCTC TATGTGTCTG GTGGCCGCAC CCAGCACGAC 
CTCTTCTCCT CCGGCCTCTT CCGTTTCCGC CTCGACGGCA CCAGTGGGGG CTATTGGGAG 
CAGGTGATGC CAGCAGGTGG GCGGCCCCCT GCCGCCACCG GCCATTCCAT GGTGTTCCAT 
GCCGCCTCCC GTGCCCTGCT GGTTCACGGT GGACACCGGC CCTCCACTGC CCGATTCTCT 
GTGCGGGTGA ACTCCACCGA GCTCTTCCAT GTGGATCGGC GCGTGTGGAC CACCCTGAAG 
GGCCGGGAGG GACTTCACGG CCCACGGGAG CGAGCCTTCC ACACGGCCAG CGTCCTGGGC 
AACTACATGC TGGTCTATGG GGGCAACGTG CACACCCACT ACCAGGAGGA AAAGTGCTAT 
GAAGACGGCA TCTTCTTCTA CCACCTGGGC TGCCATCAGT GGGTGTCAGG GGCTGAGCTT 
GCCCCTCCAG GAACTCCAGA GGGCCGAGCA GCGCCTCCTA GTGGCCGGTA CTCACACGTG 
GCGGCTGTGC TTGGTGGCAG TGTCCTGTTG GTGGCCGGGG GGTACAGTGG CCGGCCTCGT 
GGGGACTTGA TGGCCTACAA AGTGCCCCCC TTTGTGTTCC AGGCGCCTGC TCCAGATTAC 
CACTTGGACT ATTGCTCCAT GTACACGGAC CACAGCGTCT GCTCCCGAGA CCCCGAATGC 
AGTTGGTGTC AGGGGGCCTG CCAAGCCGCC CCACCTCCTG GGACTCCCTC AGGGGCTTGT 
CCGGCTGCCA GCTGCCTGGG CCTGAGCCGT CTCCTGGGTG ACTGCCAGGC CTGCCTGGCG 
TTCAGCAGCA GTGCGGCTCC CCCCCAGGGG CCCGGTGCCC TGGGCTGGTG CGTGCACAAT 
GAGAGCTGCC TCCCTCGGCC TGAGCAGGCC CGCTGCCGCG GGGAGCAGAT CTCCGGCACC 
GTGGGCTGGT GGGGGCCCGC TCCTGTCTTT GTGACCTCCC TGGAGGCCTG TGTCACCCAG 
AGCTTCCTGC CTGGCCTGCA CCTGCTCACC TTCCAGCAGC CACCCAACGC CTCCCAGCCT 
GACAAGGTCT TGATCGTCCG CAGCACGACC ATCACCCTGA CGCCGAGCTC AGAGACCGAC 
GTGTCCCTGG TCTACCGTGG CTTCATCCAC CCGCTGCTGC CGGGAGGCCC TGGGGGGTCT 
GGGGCTGAGG ATGTGGCCGT GTGGGCCCGG GCCCAGCGCC TGCATGTGCT GGCCCGGATG 
GCCCGGGGCC CGGACACAGA AAACATGGAG GAGGTGGGGC GCTGGGTGGC ACAGCAGGAG 
AAGGAGACGA GGCGGCTGCA GCGCCCAGGG TCAGCTCGCC TCTTCCCACT GCCCGGCCGG 
GGCCACAAGT ATGCAGTAGA GATCCGGGGC CAGCTCAATG GCTCAGCAGG CCCCGGGCAC 
AGTGAGCTCA CCCTGCTGTG GGACCGGACT GGCGTGCCAG GTGGCAGCGA GATCTCCTTC 
TTCTTCCTGG AGCCCTACCG CTCGTCGGCC TGCGCCTCCT ACTCCTCCTG CCTGGGCTGC 
CTGGCAGACC AGGGCTGCGG CTGGTGCCTG GCCAGCGCCA CCTGCCACCT GCGCCAGAGC 
GGCGCCCACT GTAGGGACGC TGGGGCCGGC GGGTCCCTGC TCGTGCTGGT GCCTGCCCTC 
TGCCCGCTCT GCGAGGAGCA TCGCGACTGC CACGCCTGCA CCCAGGACCC CTTCTGTGAG 
TGGCATCAGA GCACCAGCCG CAAGGGGGAC GCGGCGTGCA GCCGGCGCGG CCGGGGTCGG 
GGCGCCCTGA AGAGTCCGGA AGAGTGCCCC CCACTCTGCA GCCAGCGCCT GACCTGTGAC 
GACTGCCTGG CCAACTCGAG CCAGTGCGCC TGGTGCCAGT CCACCCGCAC CTGCTTCCTG 
TTCGCCGCCT ACCTGGCCCG GTACCCGCAC GGGGGCTGCC GAGGCTGGGA CGACAGCGTG 
CACTCGGAGC CTCGGTGCCG GAGCTGTGAC CGCTTCCCGA GCTGTCACGA GTGTCTGCAG 
AGCCATGAGT GTGGCTGGTG TGGAAACGAG GACAACCCCA CGCTGGGCCG TTGCCTCCAA 
GGGGACTTCT CAGGGCCTCT GAGTGGGGGG AACTGCTCCC TGTGGGTCGG GGAGGGCCTG 
GGGCTGTCTG TGGCCCTCCC CGCCCGCTGG GCGTATGCCC GCTGTCCGGA TGTAGACGAG 
TGCCGCCTGG GCCTGGCGCG GTGCCACCTG CGGGCGACCT GCCTGAACAC ACCCCTCAGC 
TACGAGTGTC ACTGCCAGAG GGGCTACCAG GGCGATGGCG TCACTTACTG CAACCGCACG 
TGCCTGGAGG ACTGTGGCCA CGGTGTGTGC AGCGGCCCCC CAGACTTCAC CTGCGTGTGT 
GACCTGGGCT GGACATCAGA CCTGCCCCTT CCCACGCCGG CCCCCGGCCC CCCCGCCCCC 
CGCTGTTCGC GGGACTGTGG CTGCAACTTC CACAGCCACT GCCACAAGCG GGGGCCCGGC 
TTCTGCGATG AGTGCCAGGA CTGGACGTGG GGGGAGCACT GTGAGCGGTG CCGGCCTGGA 
AGCTTCGGGA ATGCCACAGG CTCCGGCGGC TGCCGGCCCT GCCAGTGCAA CGGGCATGGG 
GACCCTCGCC GAGGCCACTG TGACAACCTC AGCGGGCTCT GCTTCTGCCA GGACCACACC 
GAGGGTGCCC ACTGCCAGCT CTGCTCCCCT GGCTACTACG GGGACCCTCG GGCAGGTGGC 
TCCTGCTTCC GGGAGTGTGG GGGTCGTGCC CTCCTCACCA ACGTGTCCTC AGTGGCACTG 
GGCTCACGCC GGTTTGGGGG GCTCCTGCCT GCAGGTGGCG GGGCAGCAAG AGCGGGGCCT 
GGCCTGTCCT ACTGTGTGTG GGTTGTCTCG GCCACTGAGG TGCTGCAGCC CTGTGCCCCC 
GGGACCCTCT GTCCCCCGCT TACCCTCACC TTCTCCCCCG ACAGCAGCAC CCCGTGCACG 
CTGAGCTATG TCCTGGCCTT TGATGGATTC CCACGCTTCC TAGACACTGG TGTCGTCCAG 
TCGGACCGTA GCCTCATCGC TGCGTTTTGC GGCCAGCGGC GGGACCGGCC CCTCACTGTG 
CAGGCCCTGT CTGGGCTGCT GGTACTGCAC TGGGAGGCCA ATGGCTCCTC ATCCTGGGGC 
TTCAATGCCT CCGTGGGCTC TGCCCGCTGT GGGTCAGGGG GCCCCGGGAG CTGCCCTGTC 
CCCCAGGAGT GTGTGCCCCA GGATGGGGTC CCAGGTGCTG GACTCTGCCG GTGTCCCCAG 
GGCTGGGCTG GCCCGCACTG CCGCATGGCT GTGTGTCCTG AGAACTGTAA TGCCCACAAC 
GGGGCGGGGA CGTGTAATCA GAGCCTGGGC GTGTGCGTCT GTGCTGAGGG CTTTGGGGGT 
CCTGACTGTG CCACGAAGCT GGATGGTGGG CAGCTGGTCT GGGAGACCCT CATGGACAGC 
CGCCTCTCAG CTGACACTGC CAGCCGCTTC CTGCACCGCC TGGGGCACAC GATGGTGGAG 
GGACCTGATG CCACCTTGTG GATGTTTGGG GGCCTGGGCC TGCCCCAGGG GCTGCTGGGA 
AACCTGTATA GGTACTCAGT GAGTGAACGG CGGTGGACAC AGATGTTGGC AGGAGCCGAG 
GATGGGGGCC CAGGCCCCTC CCCTCGCTCT TTCCACGCTG CTGCCTACGT GCCTGCTGGC 
CGTGGGGCCA TGTACCTGCT GGGGGGGCTC ACAGCTGGGG GCATCGCCCG GGACTTCTGG 
GTCCTCAACC TCACCACCCT GCAGTGGCGG CAGGAGAAGG CCCCTCAGAC TGTGGAGCTG 
CCAGCCGTTG CTGGTCACAC GCTTACTGCC CGCCGAGGCC TATCTCTGCT CCTGGTGGGT 
GGTTACTCCC CGGAAAATGG CTTCAACCAG CAGCTGCTTG AGTACCAGCT GGCGACTGGC 
ACCTGGGTGT CCGGGGCTCA GAGCGGGACA CCCCCCACAG GTCTCTACGG TCACTCTGCT 
GTGTATCATG AGGCCACCGA CTCCCTCTAC GTGTTCGGGG GTTTCCGCTT CCACGTGGAG 
CTGGCAGCCC CGTCCCCTGA GCTCTACTCC CTGCACTGCC CTGACCGCAC CTGGAGCCTG 
CTGGCCCCTT CTCAGGGAGC AAAGCCCCGG CCCCGACTCT TCCACGCCTC GGCCTTGCTA 
GGGGACACCA TGGTGGTCCT CGGGGGGCGC TCAGACCCCG ACGAGTTCAG CAGCGACGTG 
CTGCTCTACC AGGTCAACTG CAACACCTGG CTCCTGCCCG ACCTCATCGG CCCAGCCTCT 
GTGGGACCGC CAATGGAAGA GTCTGTGGCT CACGCCGTGG CCGCGGTAGG GGGTCACCTG 
TTTATCTCAG GAGGCTTTGG GGGAGTGGCC CTGGGCCGCC TGCTGGCCCT GACCCTGCCC 
CCTGACCCCT GCCGCCTGCT GCCCTCGCCC GAAGCTTGTA ACCAGACTGG GGCCTGTACC 
TGGTGCCATG GAGCCTGCCT GTCTGGGGAC CAGGCCCACA GGCTGGGCTG TGGCGGCACC 
CCCTGCTCCC CTATGCCCCA CTCCCCCGAG GAATGTCGAC GTCTGCGGAC CTGCAGCGAG 
TGCCTGGCCC GCCATCCCCG GACCCTGCAG CCTGGAGATG GAGAGGCCTC TGCCCCACGC 
TGTAAGTGGT GTACCAACTG CCCCGAGGGT GCCTGCATTG GGCGCAACGG GTCCTGCACC 
TCAGAGAATG ACTGTCGCAT CAACCAGCGC GAGGTCTTCT GGGCCGGGAA CTGCTCGGAG 
GCTGTGTGCG GGGCGGCCGA CTGCGAGCAG TGCACCAGGG AGGGCAAATG CATGTGGACG 
CGGCAGTTCA AGAGGACGGG GGAGACCCGC CGCATCCTTT CGGTGCAGCC CACCTACGAC 
TGGACGTGCT TCAGCCACTC ACTGCTGAAC GTGTCCCCGA TGCCAGTGGA GTCATCGCCC 
CCGCTGCCCT GCCCCACACC CTGTCACCTC CTGCCCAACT GCACCTCCTG CCTGGACTCC 
AAGGGTGCTG ACGGGGGCTG GCAGCACTGC GTCTGGAGCA GCAGCCTCCA GCAGTGTCTG 
AGCCCCTCCT ATCTGCCCCT GCGGTGTATG GCCGGAGGCT GTGGGCGGCT GCTCCGCGGA 
CCCGAGAGCT GCTCCCTGGG CTGTGCTCAG GCAACCCAGT GTGCCCTGTG CCTGCGGCGC 
CCCCACTGTG GCTGGTGTAC CTGGGGGGGC CAGGATGGGG GCGGCCGCTG CCTGGAGGGT 
GGACTCAGTG GCCCCCGTGA CGGGCTGACG TGTGGGCGAC CTGGGGCCTC CTGGGCCTTT 
CTTTCCTGCC CCCCGGAGGA CGAATGTGCC AACGGGCACC ATGACTGCAA CGAGACCCAG 
AACTGCCACG ACCGGCCCCA CGGTTACGAG TGCAGCTGCA AGGCAGGCTA CACCATGGAC 
AACGTGACGG GGCTGTGCCG CCCAGTGTGC GCCCAGGGTT GCGTGAACGG CTCGTGTGTG 
GAGCCCGACC ACTGCCGCTG CCACTTCGGC TTCGTCGGCC GCAACTGTTC CACGGAGTGT 
CGCTGCAACC GCCACAGCGA CTGCGCCGGC GTGGGGGCCC GCGACCACTG CCTGCTCTGC 
CACAATCACA CCAAGGGCAG CCACTGTGAG CAGTGCCTCC CGCTGTTCGT GGGCTCAGCC 
GTAGGGGGAG GGACCTGCCG GCCCTGCCAC GCCTTTTGTC GCGGAAACAG CCACGTCTGC 
GTCTCCAGGA AGGAGCTTGA GATAGCCAGG AGGGAGCCGG AGAAGTACTC GCTGGATCCA 
GATGAGATTG AACACTGGGC GACAGAGGGT CCCAGCGAAG ATGAGGCTGT GTGTGTGAAC 
TGCCAGAATA ACAGCTATGG GGATAAGTGT GAGAGCTGCC TCCACGGCTA CTTCCTCCTC 
GACGGGAAAT GCACCAAATG CCAGTGTAAT GGCCACGCGG ACACATGTAA TGAGCAGGAT 
GGGACAGGCT GCCCATGTCA GAACAACACA GAGACAGGCA CGTGCCAGGG CAGCTCCCCC 
AGTGACCGCC GAGACTGCTA CAAATACCAG TGTGCCAAGT GCCGAGAGTC ATTCCACGGG 
AGCCCGCTAG GGGGCCAGCA GTGCTACCGC CTCATCTCCG TGGAACAGGA GTACTGCCTG 
GACCCCACGT CCCAAACCAA CTGTTTCCAC GAGCCCAAGC GCCGGGCTCT GGGTCCCGGA 
CGCACCGTTC TCTTTGGAGT GCAGCCTAAG TTCACCAACG TGGACATCCG CCTGACGCTG 
GACGTGACCT TTGGCGCCGT GGACCTCTAC GTCTCCACCT CCTATGACAC CTTCGTGGTC 
CGCGTGGCTC CCGACACTGG TGTCCACACC GTGCATATCC AGCCGCCACC ACCTCCCCCA 
CCCCCACCAC CCCCTGCTGA CGGCGGGCCC CGGGGACCTG GGGATCTCGG GGGACCTGGG 
GCTGGAGGTG GGCCGGCTAC CCCAGCAGAG CCGCGAGTGC GGGAGGTCTG GCCACGGGGC 
CTGATCACCT ACGTGACAGT GACAGAGCCG TCGGCGGTGC TGGTGGTCCG TGGCGTGAGG 
GACCGGCTGG TCATCACCTA CCCGCACGAG CACCACACCC TCAAGTCCAG CCGCTTCTAC 
CTGCTCCTGC TGGGTGTGGG AGACCCAAGT GGGCCCAGTG CCAACGGCTC AGCTGACTCG 
CAGGGCTTGC TGTTCTTCCG GCAGGACCAG GCCCACATTG ACCTGTTTGT CTTCTTCTCT 
GTCTTCTTCT CCTGCTTCTT CCTCTTCCTC TCGCTCTGTG TGCTGCTCTG GAAGGCCAAG 
CAGGCCCTGG ACCACCGGCA GGAACAGCGC CGGCACCTGC AGGAGATGAC CAAGATGGCC 
AGCCGCCCCT TCGCCAAGGT CACTGTCTGC TTCCCGCCAG ACCCTGCTGC CCCAGCCCCC 
ACCTGGAAGC CTGCCGGGCT CCCGCCACCC GCCTTCCGCC GCTCCGAGCC CTTCTTGGCA 
CCCTTGCTGC TGACCGGGGC CGGCGGGCCC TGGGGACCTG TAGGAGGGGG CTGCTGCCCA 
CCGGCCATCC CCACCACCAC TGCCGGCCTG CGAGCCGGGC CTATCACCCT TGAGCCCACC 
GAGGATGGCA TGGCCGGCGT GGCCACACTG CTGCTCCAAC TGCCTGGCGG GCCCCACGCA 
CCCAACGGCG CCTGCCTGGG GTCAGCGCTC GTCACACTGC GGCACCGGCT GCACGAATAC 
TGCGGCGGCG GCGGGGGCGC TGGGGGCAGT GGGCATGGGG CTGGTGCGGG CCGGAAGGGG 
CTGTTGAGCC AGGACAACCT CACCAGCATG TCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF8 ( XM_015467508.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322994.1 Bos taurus multiple EGF like domains 8 (MEGF8), mRNA.

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CloneID	OBa93525
Clone ID Related Accession (Same CDS sequence)	XM_015467508.1 , XM_015458238.1
Accession Version	XM_015467508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	multiple epidermal growth factor-like domains protein 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END##

ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTGTAT CATTGGCCGT GCTGGGGCCA 
CTGTCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT 
CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC 
ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA 
TGCACTTATG ACTACCTGTT CGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC 
AGTCTGAGTG GGAGCACACG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTG 
CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC 
CTGTGCCCGG GTGGCTGCCG CAGCCATGGG CAGTGCCAGC CCCCAGGGGT GTGTGTCTGT 
GAGCCGGGCT GGGGGGGCCC CGACTGCAGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC 
CATGGCACCT GCACCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGC 
GCCTGTGACC TGCACCTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC 
GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACTGGGG 
CTGCTGGCTG TTTTTGGAGG CCAGGACCTC AACCGCGCGC TGGGGGACCT CGTCCTGTAC 
AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC 
TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT 
GGCACCCTCA CCAGCGACAT CTGGGCCTTT AGCCCGATGG GTGGCGGCCG CTGGGAGCTT 
CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA TGCAGCAGCC 
CTGGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC 
TCCGGCCTCT TCCGTTTCCG CCTCGACGGC ACCAGTGGGG GCTATTGGGA GCAGGTGATG 
CCAGCAGGTG GGCGGCCCCC TGCCGCCACC GGCCATTCCA TGGTGTTCCA TGCCGCCTCC 
CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG 
AACTCCACCG AGCTCTTCCA TGTGGATCGG CGCGTGTGGA CCACCCTGAA GGGCCGGGAG 
GGACTTCACG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG 
CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC 
ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCTGAGCT TGCCCCTCCA 
GGAACTCCAG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG 
CTTGGTGGCA GTGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCTCG TGGGGACTTG 
ATGGCCTACA AAGTGCCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGATTA CCACTTGGAC 
TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGTTGGTGT 
CAGGGGGCCT GCCAAGCCGC CCCACCTCCT GGGACTCCCT CAGGGGCTTG TCCGGCTGCC 
AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC 
AGTGCGGCTC CCCCCCAGGG GCCCGGTGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC 
CTCCCTCGGC CTGGTAAGTG TCCACAGCAC CCCGTGCACG ACACTCGTGA CAGCTCGGGA 
AGCGGTCACA GCTCCGGCAC CGAGGCTCCG AGTGCACGCT GTGGGGAGGC GGGTGTGAGG 
CCGCCCGAGC TACCCCCGCC CCGCCCCACC TCCACCCTCC CCTTCCGCGC CCCTGCCGCC 
CGCCCGCACC TGTCGTCCCA GCCTCGGCAG CCCCCGTGCG GGTACCGGGC CAGCGGAGCT 
CACTGGCTGC AGAGTGGGGG GCACTCTTCC GGACTCTTCA GGGCGCCCCG ACCCCGGCCG 
CGCCGGCTGC ACGCCGCGTC CCCCTTGCGG CTGCCTGGTG CGCACCTGGG TGCAGGCGTG 
GCAGTCGCGA TGCTCCTCGC AGAGCGGGCA GAGGGCAGGC ACCAGCACGA GCAGGGACCC 
GCCGGCCCCA GCGTCCCTAC AGTGGGCGCC GCTCTGGCGC AGGTGGCAGG TGGCGCTGGC 
CAGGCACCAG CCGCAGCCCT GGTCTGCCAG GCAGCCCAGG CAGGAGGAGT AGGAGGCGCA 
GGCCGACGAG CGGTAGGGCT CCAGGAAGAA GAAGGAGATC TCCTGGGGGT GAGGGTGTCA 
GAGGAGGGTG AGGGCTCCAT GCTCCAGGTC AAGCCTGCTG AGTGTCCCAG GCATTCTTAC 
CATGTTTTCT GTGTCCGGGC CCCGGGCCAT CCGGGCCAGC ACATGCAGGC GCTGGGCCCG 
GGCCCACACG GCCACATCCT CAGCCCCAGA CCCCCCAGGG CCTCCCGGCA GCAGCGGGTG 
GATGAAGCCA CGTCTGCCTC TCTTCCATTT CTCCTGTCTG TCTCCATATT CATCTTTGTG 
TCTCTCTGGT TTATCTCCTC TCTCCCTGTC CCCGTCTCTC TGTCTCTCTC CTTACTTTGC 
CCCTCCACCC CAATCGCCTT CTTCAACCCC ACTCCCCGCA GTTGCCTCCA AGGGGACTTC 
TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT 
GTGGCCCTCC CCGCCCGCTG GGCGTATGCC CGCTGTCCGG ATGTAGACGA GTGCCGCCTG 
GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTACGAGTGT 
CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG 
GACTGTGGCC ACGGTGTGTG CAGCGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC 
TGGACATCAG ACCTGCCCCT TCCCACGCCG GCCCCCGGCC CCCCCGCCCC CCGCTGTTCG 
CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAT 
GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCTGG AAGCTTCGGG 
AATGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ACGGGCATGG GGACCCTCGC 
CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC 
CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC 
CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC 
CGGTTTGGGG GGCTCCTGCC TGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC 
TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTGCAGC CCTGTGCCCC CGGGACCCTC 
TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAT 
GTCCTGGCCT TTGATGGATT CCCACGCTTC CTAGACACTG GTGTCGTCCA GTCGGACCGT 
AGCCTCATCG CTGCGTTTTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG 
TCTGGGCTGC TGGTACTGCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAATGCC 
TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCCGGGA GCTGCCCTGT CCCCCAGGAG 
TGTGTGCCCC AGGATGGGGT CCCAGGTGCT GGACTCTGCC GGTGTCCCCA GGGCTGGGCT 
GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG 
ACGTGTAATC AGAGCCTGGG CGTGTGCGTC TGTGCTGAGG GCTTTGGGGG TCCTGACTGT 
GCCACGAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA 
GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT 
GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT 
AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCCGA GGATGGGGGC 
CCAGGCCCCT CCCCTCGCTC TTTCCACTCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC 
ATGTACCTGC TGGGGGGGCT CACAGCTGGG GGCATCGCCC GGGACTTCTG GGTCCTCAAC 
CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCCGTT 
GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC 
CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG 
TCCGGGGCTC AGAGCGGGAC ACCCCCCACA GGTCTCTACG GTCACTCTGC TGTGTATCAT 
GAGGCCACCG ACTCCCTCTA CGTGTTCGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC 
CCGTCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT 
TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCACGCCT CGGCCTTGCT AGGGGACACC 
ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGCGACGT GCTGCTCTAC 
CAGGTCAACT GCAACACCTG GCTCCTGCCC GACCTCATCG GCCCAGCCTC TGTGGGACCG 
CCAATGGAAG AGTCTGTGGC TCACGCCGTG GCCGCGGTAG GGGGTCACCT GTTTATCTCA 
GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCC TGACCCTGCC CCCTGACCCC 
TGCCGCCTGC TGCCCTCGCC CGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT 
GGAGCCTGCC TGTCTGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCAC CCCCTGCTCC 
CCTATGCCCC ACTCCCCCGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC 
CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCT CTGCCCCACG CTGTAAGTGG 
TGTACCAACT GCCCCGAGGG TGCCTGCATT GGGCGCAACG GGTCCTGCAC CTCAGAGAAT 
GACTGTCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCGGA GGCTGTGTGC 
GGGGCGGCCG ACTGCGAGCA GTGCACCAGG GAGGGCAAAT GCATGTGGAC GCGGCAGTTC 
AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC 
TTCAGCCACT CACTGCTGAA CGTGTCCCCG ATGCCAGTGG AGTCATCGCC CCCGCTGCCC 
TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT 
GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC 
TATCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC 
TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGTGCCCTGT GCCTGCGGCG CCCCCACTGT 
GGCTGGTGTA CCTGGGGGGG CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT 
GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CCTGGGCCTT TCTTTCCTGC 
CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ACGAGACCCA GAACTGCCAC 
GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG 
GGGCTGTGCC GCCCAGTGTG CGCCCAGGGT TGCGTGAACG GCTCGTGTGT GGAGCCCGAC 
CACTGCCGCT GCCACTTCGG CTTCGTCGGC CGCAACTGTT CCACGGAGTG TCGCTGCAAC 
CGCCACAGCG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAATCAC 
ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCGCTGTTCG TGGGCTCAGC CGTAGGGGGA 
GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGAAACA GCCACGTCTG CGTCTCCAGG 
AAGGAGCTTG AGATAGCCAG GAGGGAGCCG GAGAAGTACT CGCTGGATCC AGATGAGATT 
GAACACTGGG CGACAGAGGG TCCCAGCGAA GATGAGGCTG TGTGTGTGAA CTGCCAGAAT 
AACAGCTATG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTCCTCCT CGACGGGAAA 
TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACATGTA ATGAGCAGGA TGGGACAGGC 
TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC 
CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA 
GGGGGCCAGC AGTGCTACCG CCTCATCTCC GTGGAACAGG AGTACTGCCT GGACCCCACG 
TCCCAAACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGTCCCGG ACGCACCGTT 
CTCTTTGGAG TGCAGCCTAA GTTCACCAAC GTGGACATCC GCCTGACGCT GGACGTGACC 
TTTGGCGCCG TGGACCTCTA CGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT 
CCCGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CACCTCCCCC ACCCCCACCA 
CCCCCTGCTG ACGGCGGGCC CCGGGGACCT GGGGATCTCG GGGGACCTGG GGCTGGAGGT 
GGGCCGGCTA CCCCAGCAGA GCCGCGAGTG CGGGAGGTCT GGCCACGGGG CCTGATCACC 
TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG 
GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG 
CTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG 
CTGTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC TGTCTTCTTC 
TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG 
GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC 
TTCGCCAAGG TCACTGTCTG CTTCCCGCCA GACCCTGCTG CCCCAGCCCC CACCTGGAAG 
CCTGCCGGGC TCCCGCCACC CGCCTTCCGC CGCTCCGAGC CCTTCTTGGC ACCCTTGCTG 
CTGACCGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGGG GCTGCTGCCC ACCGGCCATC 
CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC 
ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGCG GGCCCCACGC ACCCAACGGC 
GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAATA CTGCGGCGGC 
GGCGGGGGCG CTGGGGGCAG TGGGCATGGG GCTGGTGCGG GCCGGAAGGG GCTGTTGAGC 
CAGGACAACC TCACCAGCAT GTCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322994.1
CDS	1064..9193
Translation	MALGRALAVALAVSLAVLGPLSPGAQAGDCKGQRQVLREAPGFVTDGAGNYSVNGNCEWLIEAPSSQHRILLDFLFLDTECTYDYLFVYDGDSPRGPLLASLSGSTRPPPIEASSGKMLLHLFSDANYNLLGFNASFRFSLCPGGCRSHGQCQPPGVCVCEPGWGGPDCSLQECSAYCGSHGTCTSPLGPCRCEPGFLGRACDLHLWENQGAGWWHNVSAGDPAFSARIGAAGAFLSPLGLLAVFGGQDLNRALGDLVLYNFSANTWERWDLSPAPAARHSHVAVAWAGSLVLMGGELADGTLTSDIWAFSPMGGGRWELLAPPASSPSGPPGLAGHAAALVDNTWLYVSGGRTQHDLFSSGLFRFRLDGTSGGYWEQVMPAGGRPPAATGHSMVFHAASRALLVHGGHRPSTARFSVRVNSTELFHVDRRVWTTLKGREGLHGPRERAFHTASVLGNYMLVYGGNVHTHYQEEKCYEDGIFFYHLGCHQWVSGAELAPPGTPEGRAAPPSGRYSHVAAVLGGSVLLVAGGYSGRPRGDLMAYKVPPFVFQAPAPDYHLDYCSMYTDHSVCSRDPECSWCQGACQAAPPPGTPSGACPAASCLGLSRLLGDCQACLAFSSSAAPPQGPGALGWCVHNESCLPRPGKCPQHPVHDTRDSSGSGHSSGTEAPSARCGEAGVRPPELPPPRPTSTLPFRAPAARPHLSSQPRQPPCGYRASGAHWLQSGGHSSGLFRAPRPRPRRLHAASPLRLPGAHLGAGVAVAMLLAERAEGRHQHEQGPAGPSVPTVGAALAQVAGGAGQAPAAALVCQAAQAGGVGGAGRRAVGLQEEEGDLLGVRVSEEGEGSMLQVKPAECPRHSYHVFCVRAPGHPGQHMQALGPGPHGHILSPRPPRASRQQRVDEATSASLPFLLSVSIFIFVSLWFISSLPVPVSLSLSLLCPSTPIAFFNPTPRSCLQGDFSGPLSGGNCSLWVGEGLGLSVALPARWAYARCPDVDECRLGLARCHLRATCLNTPLSYECHCQRGYQGDGVTYCNRTCLEDCGHGVCSGPPDFTCVCDLGWTSDLPLPTPAPGPPAPRCSRDCGCNFHSHCHKRGPGFCDECQDWTWGEHCERCRPGSFGNATGSGGCRPCQCNGHGDPRRGHCDNLSGLCFCQDHTEGAHCQLCSPGYYGDPRAGGSCFRECGGRALLTNVSSVALGSRRFGGLLPAGGGAARAGPGLSYCVWVVSATEVLQPCAPGTLCPPLTLTFSPDSSTPCTLSYVLAFDGFPRFLDTGVVQSDRSLIAAFCGQRRDRPLTVQALSGLLVLHWEANGSSSWGFNASVGSARCGSGGPGSCPVPQECVPQDGVPGAGLCRCPQGWAGPHCRMAVCPENCNAHNGAGTCNQSLGVCVCAEGFGGPDCATKLDGGQLVWETLMDSRLSADTASRFLHRLGHTMVEGPDATLWMFGGLGLPQGLLGNLYRYSVSERRWTQMLAGAEDGGPGPSPRSFHSAAYVPAGRGAMYLLGGLTAGGIARDFWVLNLTTLQWRQEKAPQTVELPAVAGHTLTARRGLSLLLVGGYSPENGFNQQLLEYQLATGTWVSGAQSGTPPTGLYGHSAVYHEATDSLYVFGGFRFHVELAAPSPELYSLHCPDRTWSLLAPSQGAKPRPRLFHASALLGDTMVVLGGRSDPDEFSSDVLLYQVNCNTWLLPDLIGPASVGPPMEESVAHAVAAVGGHLFISGGFGGVALGRLLALTLPPDPCRLLPSPEACNQTGACTWCHGACLSGDQAHRLGCGGTPCSPMPHSPEECRRLRTCSECLARHPRTLQPGDGEASAPRCKWCTNCPEGACIGRNGSCTSENDCRINQREVFWAGNCSEAVCGAADCEQCTREGKCMWTRQFKRTGETRRILSVQPTYDWTCFSHSLLNVSPMPVESSPPLPCPTPCHLLPNCTSCLDSKGADGGWQHCVWSSSLQQCLSPSYLPLRCMAGGCGRLLRGPESCSLGCAQATQCALCLRRPHCGWCTWGGQDGGGRCLEGGLSGPRDGLTCGRPGASWAFLSCPPEDECANGHHDCNETQNCHDRPHGYECSCKAGYTMDNVTGLCRPVCAQGCVNGSCVEPDHCRCHFGFVGRNCSTECRCNRHSDCAGVGARDHCLLCHNHTKGSHCEQCLPLFVGSAVGGGTCRPCHAFCRGNSHVCVSRKELEIARREPEKYSLDPDEIEHWATEGPSEDEAVCVNCQNNSYGDKCESCLHGYFLLDGKCTKCQCNGHADTCNEQDGTGCPCQNNTETGTCQGSSPSDRRDCYKYQCAKCRESFHGSPLGGQQCYRLISVEQEYCLDPTSQTNCFHEPKRRALGPGRTVLFGVQPKFTNVDIRLTLDVTFGAVDLYVSTSYDTFVVRVAPDTGVHTVHIQPPPPPPPPPPPADGGPRGPGDLGGPGAGGGPATPAEPRVREVWPRGLITYVTVTEPSAVLVVRGVRDRLVITYPHEHHTLKSSRFYLLLLGVGDPSGPSANGSADSQGLLFFRQDQAHIDLFVFFSVFFSCFFLFLSLCVLLWKAKQALDHRQEQRRHLQEMTKMASRPFAKVTVCFPPDPAAPAPTWKPAGLPPPAFRRSEPFLAPLLLTGAGGPWGPVGGGCCPPAIPTTTAGLRAGPITLEPTEDGMAGVATLLLQLPGGPHAPNGACLGSALVTLRHRLHEYCGGGGGAGGSGHGAGAGRKGLLSQDNLTSMSL

Target ORF information:

RefSeq Version	XM_015467508.1
Organism	Bos taurus(cattle)
Definition	Bos taurus multiple EGF like domains 8 (MEGF8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015467508.1

ORF Insert Sequence:

ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTGTAT CATTGGCCGT GCTGGGGCCA 
CTGTCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT 
CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC 
ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA 
TGCACTTATG ACTACCTGTT CGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC 
AGTCTGAGTG GGAGCACACG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTG 
CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC 
CTGTGCCCGG GTGGCTGCCG CAGCCATGGG CAGTGCCAGC CCCCAGGGGT GTGTGTCTGT 
GAGCCGGGCT GGGGGGGCCC CGACTGCAGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC 
CATGGCACCT GCACCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGC 
GCCTGTGACC TGCACCTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC 
GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACTGGGG 
CTGCTGGCTG TTTTTGGAGG CCAGGACCTC AACCGCGCGC TGGGGGACCT CGTCCTGTAC 
AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC 
TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT 
GGCACCCTCA CCAGCGACAT CTGGGCCTTT AGCCCGATGG GTGGCGGCCG CTGGGAGCTT 
CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA TGCAGCAGCC 
CTGGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC 
TCCGGCCTCT TCCGTTTCCG CCTCGACGGC ACCAGTGGGG GCTATTGGGA GCAGGTGATG 
CCAGCAGGTG GGCGGCCCCC TGCCGCCACC GGCCATTCCA TGGTGTTCCA TGCCGCCTCC 
CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG 
AACTCCACCG AGCTCTTCCA TGTGGATCGG CGCGTGTGGA CCACCCTGAA GGGCCGGGAG 
GGACTTCACG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG 
CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC 
ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCTGAGCT TGCCCCTCCA 
GGAACTCCAG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG 
CTTGGTGGCA GTGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCTCG TGGGGACTTG 
ATGGCCTACA AAGTGCCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGATTA CCACTTGGAC 
TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGTTGGTGT 
CAGGGGGCCT GCCAAGCCGC CCCACCTCCT GGGACTCCCT CAGGGGCTTG TCCGGCTGCC 
AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC 
AGTGCGGCTC CCCCCCAGGG GCCCGGTGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC 
CTCCCTCGGC CTGGTAAGTG TCCACAGCAC CCCGTGCACG ACACTCGTGA CAGCTCGGGA 
AGCGGTCACA GCTCCGGCAC CGAGGCTCCG AGTGCACGCT GTGGGGAGGC GGGTGTGAGG 
CCGCCCGAGC TACCCCCGCC CCGCCCCACC TCCACCCTCC CCTTCCGCGC CCCTGCCGCC 
CGCCCGCACC TGTCGTCCCA GCCTCGGCAG CCCCCGTGCG GGTACCGGGC CAGCGGAGCT 
CACTGGCTGC AGAGTGGGGG GCACTCTTCC GGACTCTTCA GGGCGCCCCG ACCCCGGCCG 
CGCCGGCTGC ACGCCGCGTC CCCCTTGCGG CTGCCTGGTG CGCACCTGGG TGCAGGCGTG 
GCAGTCGCGA TGCTCCTCGC AGAGCGGGCA GAGGGCAGGC ACCAGCACGA GCAGGGACCC 
GCCGGCCCCA GCGTCCCTAC AGTGGGCGCC GCTCTGGCGC AGGTGGCAGG TGGCGCTGGC 
CAGGCACCAG CCGCAGCCCT GGTCTGCCAG GCAGCCCAGG CAGGAGGAGT AGGAGGCGCA 
GGCCGACGAG CGGTAGGGCT CCAGGAAGAA GAAGGAGATC TCCTGGGGGT GAGGGTGTCA 
GAGGAGGGTG AGGGCTCCAT GCTCCAGGTC AAGCCTGCTG AGTGTCCCAG GCATTCTTAC 
CATGTTTTCT GTGTCCGGGC CCCGGGCCAT CCGGGCCAGC ACATGCAGGC GCTGGGCCCG 
GGCCCACACG GCCACATCCT CAGCCCCAGA CCCCCCAGGG CCTCCCGGCA GCAGCGGGTG 
GATGAAGCCA CGTCTGCCTC TCTTCCATTT CTCCTGTCTG TCTCCATATT CATCTTTGTG 
TCTCTCTGGT TTATCTCCTC TCTCCCTGTC CCCGTCTCTC TGTCTCTCTC CTTACTTTGC 
CCCTCCACCC CAATCGCCTT CTTCAACCCC ACTCCCCGCA GTTGCCTCCA AGGGGACTTC 
TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT 
GTGGCCCTCC CCGCCCGCTG GGCGTATGCC CGCTGTCCGG ATGTAGACGA GTGCCGCCTG 
GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTACGAGTGT 
CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG 
GACTGTGGCC ACGGTGTGTG CAGCGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC 
TGGACATCAG ACCTGCCCCT TCCCACGCCG GCCCCCGGCC CCCCCGCCCC CCGCTGTTCG 
CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAT 
GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCTGG AAGCTTCGGG 
AATGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ACGGGCATGG GGACCCTCGC 
CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC 
CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC 
CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC 
CGGTTTGGGG GGCTCCTGCC TGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC 
TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTGCAGC CCTGTGCCCC CGGGACCCTC 
TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAT 
GTCCTGGCCT TTGATGGATT CCCACGCTTC CTAGACACTG GTGTCGTCCA GTCGGACCGT 
AGCCTCATCG CTGCGTTTTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG 
TCTGGGCTGC TGGTACTGCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAATGCC 
TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCCGGGA GCTGCCCTGT CCCCCAGGAG 
TGTGTGCCCC AGGATGGGGT CCCAGGTGCT GGACTCTGCC GGTGTCCCCA GGGCTGGGCT 
GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG 
ACGTGTAATC AGAGCCTGGG CGTGTGCGTC TGTGCTGAGG GCTTTGGGGG TCCTGACTGT 
GCCACGAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA 
GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT 
GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT 
AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCCGA GGATGGGGGC 
CCAGGCCCCT CCCCTCGCTC TTTCCACTCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC 
ATGTACCTGC TGGGGGGGCT CACAGCTGGG GGCATCGCCC GGGACTTCTG GGTCCTCAAC 
CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCCGTT 
GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC 
CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG 
TCCGGGGCTC AGAGCGGGAC ACCCCCCACA GGTCTCTACG GTCACTCTGC TGTGTATCAT 
GAGGCCACCG ACTCCCTCTA CGTGTTCGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC 
CCGTCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT 
TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCACGCCT CGGCCTTGCT AGGGGACACC 
ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGCGACGT GCTGCTCTAC 
CAGGTCAACT GCAACACCTG GCTCCTGCCC GACCTCATCG GCCCAGCCTC TGTGGGACCG 
CCAATGGAAG AGTCTGTGGC TCACGCCGTG GCCGCGGTAG GGGGTCACCT GTTTATCTCA 
GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCC TGACCCTGCC CCCTGACCCC 
TGCCGCCTGC TGCCCTCGCC CGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT 
GGAGCCTGCC TGTCTGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCAC CCCCTGCTCC 
CCTATGCCCC ACTCCCCCGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC 
CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCT CTGCCCCACG CTGTAAGTGG 
TGTACCAACT GCCCCGAGGG TGCCTGCATT GGGCGCAACG GGTCCTGCAC CTCAGAGAAT 
GACTGTCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCGGA GGCTGTGTGC 
GGGGCGGCCG ACTGCGAGCA GTGCACCAGG GAGGGCAAAT GCATGTGGAC GCGGCAGTTC 
AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC 
TTCAGCCACT CACTGCTGAA CGTGTCCCCG ATGCCAGTGG AGTCATCGCC CCCGCTGCCC 
TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT 
GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC 
TATCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC 
TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGTGCCCTGT GCCTGCGGCG CCCCCACTGT 
GGCTGGTGTA CCTGGGGGGG CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT 
GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CCTGGGCCTT TCTTTCCTGC 
CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ACGAGACCCA GAACTGCCAC 
GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG 
GGGCTGTGCC GCCCAGTGTG CGCCCAGGGT TGCGTGAACG GCTCGTGTGT GGAGCCCGAC 
CACTGCCGCT GCCACTTCGG CTTCGTCGGC CGCAACTGTT CCACGGAGTG TCGCTGCAAC 
CGCCACAGCG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAATCAC 
ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCGCTGTTCG TGGGCTCAGC CGTAGGGGGA 
GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGAAACA GCCACGTCTG CGTCTCCAGG 
AAGGAGCTTG AGATAGCCAG GAGGGAGCCG GAGAAGTACT CGCTGGATCC AGATGAGATT 
GAACACTGGG CGACAGAGGG TCCCAGCGAA GATGAGGCTG TGTGTGTGAA CTGCCAGAAT 
AACAGCTATG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTCCTCCT CGACGGGAAA 
TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACATGTA ATGAGCAGGA TGGGACAGGC 
TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC 
CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA 
GGGGGCCAGC AGTGCTACCG CCTCATCTCC GTGGAACAGG AGTACTGCCT GGACCCCACG 
TCCCAAACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGTCCCGG ACGCACCGTT 
CTCTTTGGAG TGCAGCCTAA GTTCACCAAC GTGGACATCC GCCTGACGCT GGACGTGACC 
TTTGGCGCCG TGGACCTCTA CGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT 
CCCGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CACCTCCCCC ACCCCCACCA 
CCCCCTGCTG ACGGCGGGCC CCGGGGACCT GGGGATCTCG GGGGACCTGG GGCTGGAGGT 
GGGCCGGCTA CCCCAGCAGA GCCGCGAGTG CGGGAGGTCT GGCCACGGGG CCTGATCACC 
TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG 
GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG 
CTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG 
CTGTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC TGTCTTCTTC 
TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG 
GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC 
TTCGCCAAGG TCACTGTCTG CTTCCCGCCA GACCCTGCTG CCCCAGCCCC CACCTGGAAG 
CCTGCCGGGC TCCCGCCACC CGCCTTCCGC CGCTCCGAGC CCTTCTTGGC ACCCTTGCTG 
CTGACCGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGGG GCTGCTGCCC ACCGGCCATC 
CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC 
ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGCG GGCCCCACGC ACCCAACGGC 
GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAATA CTGCGGCGGC 
GGCGGGGGCG CTGGGGGCAG TGGGCATGGG GCTGGTGCGG GCCGGAAGGG GCTGTTGAGC 
CAGGACAACC TCACCAGCAT GTCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF8 ( XM_015458238.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015313724.1 Bos taurus multiple EGF like domains 8 (MEGF8), mRNA.

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CloneID	OBa93525
Clone ID Related Accession (Same CDS sequence)	XM_015467508.1 , XM_015458238.1
Accession Version	XM_015458238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	multiple epidermal growth factor-like domains protein 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END##

ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTGTAT CATTGGCCGT GCTGGGGCCA 
CTGTCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT 
CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC 
ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA 
TGCACTTATG ACTACCTGTT CGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC 
AGTCTGAGTG GGAGCACACG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTG 
CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC 
CTGTGCCCGG GTGGCTGCCG CAGCCATGGG CAGTGCCAGC CCCCAGGGGT GTGTGTCTGT 
GAGCCGGGCT GGGGGGGCCC CGACTGCAGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC 
CATGGCACCT GCACCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGC 
GCCTGTGACC TGCACCTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC 
GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACTGGGG 
CTGCTGGCTG TTTTTGGAGG CCAGGACCTC AACCGCGCGC TGGGGGACCT CGTCCTGTAC 
AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC 
TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT 
GGCACCCTCA CCAGCGACAT CTGGGCCTTT AGCCCGATGG GTGGCGGCCG CTGGGAGCTT 
CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA TGCAGCAGCC 
CTGGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC 
TCCGGCCTCT TCCGTTTCCG CCTCGACGGC ACCAGTGGGG GCTATTGGGA GCAGGTGATG 
CCAGCAGGTG GGCGGCCCCC TGCCGCCACC GGCCATTCCA TGGTGTTCCA TGCCGCCTCC 
CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG 
AACTCCACCG AGCTCTTCCA TGTGGATCGG CGCGTGTGGA CCACCCTGAA GGGCCGGGAG 
GGACTTCACG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG 
CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC 
ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCTGAGCT TGCCCCTCCA 
GGAACTCCAG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG 
CTTGGTGGCA GTGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCTCG TGGGGACTTG 
ATGGCCTACA AAGTGCCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGATTA CCACTTGGAC 
TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGTTGGTGT 
CAGGGGGCCT GCCAAGCCGC CCCACCTCCT GGGACTCCCT CAGGGGCTTG TCCGGCTGCC 
AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC 
AGTGCGGCTC CCCCCCAGGG GCCCGGTGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC 
CTCCCTCGGC CTGGTAAGTG TCCACAGCAC CCCGTGCACG ACACTCGTGA CAGCTCGGGA 
AGCGGTCACA GCTCCGGCAC CGAGGCTCCG AGTGCACGCT GTGGGGAGGC GGGTGTGAGG 
CCGCCCGAGC TACCCCCGCC CCGCCCCACC TCCACCCTCC CCTTCCGCGC CCCTGCCGCC 
CGCCCGCACC TGTCGTCCCA GCCTCGGCAG CCCCCGTGCG GGTACCGGGC CAGCGGAGCT 
CACTGGCTGC AGAGTGGGGG GCACTCTTCC GGACTCTTCA GGGCGCCCCG ACCCCGGCCG 
CGCCGGCTGC ACGCCGCGTC CCCCTTGCGG CTGCCTGGTG CGCACCTGGG TGCAGGCGTG 
GCAGTCGCGA TGCTCCTCGC AGAGCGGGCA GAGGGCAGGC ACCAGCACGA GCAGGGACCC 
GCCGGCCCCA GCGTCCCTAC AGTGGGCGCC GCTCTGGCGC AGGTGGCAGG TGGCGCTGGC 
CAGGCACCAG CCGCAGCCCT GGTCTGCCAG GCAGCCCAGG CAGGAGGAGT AGGAGGCGCA 
GGCCGACGAG CGGTAGGGCT CCAGGAAGAA GAAGGAGATC TCCTGGGGGT GAGGGTGTCA 
GAGGAGGGTG AGGGCTCCAT GCTCCAGGTC AAGCCTGCTG AGTGTCCCAG GCATTCTTAC 
CATGTTTTCT GTGTCCGGGC CCCGGGCCAT CCGGGCCAGC ACATGCAGGC GCTGGGCCCG 
GGCCCACACG GCCACATCCT CAGCCCCAGA CCCCCCAGGG CCTCCCGGCA GCAGCGGGTG 
GATGAAGCCA CGTCTGCCTC TCTTCCATTT CTCCTGTCTG TCTCCATATT CATCTTTGTG 
TCTCTCTGGT TTATCTCCTC TCTCCCTGTC CCCGTCTCTC TGTCTCTCTC CTTACTTTGC 
CCCTCCACCC CAATCGCCTT CTTCAACCCC ACTCCCCGCA GTTGCCTCCA AGGGGACTTC 
TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT 
GTGGCCCTCC CCGCCCGCTG GGCGTATGCC CGCTGTCCGG ATGTAGACGA GTGCCGCCTG 
GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTACGAGTGT 
CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG 
GACTGTGGCC ACGGTGTGTG CAGCGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC 
TGGACATCAG ACCTGCCCCT TCCCACGCCG GCCCCCGGCC CCCCCGCCCC CCGCTGTTCG 
CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAT 
GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCTGG AAGCTTCGGG 
AATGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ACGGGCATGG GGACCCTCGC 
CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC 
CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC 
CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC 
CGGTTTGGGG GGCTCCTGCC TGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC 
TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTGCAGC CCTGTGCCCC CGGGACCCTC 
TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAT 
GTCCTGGCCT TTGATGGATT CCCACGCTTC CTAGACACTG GTGTCGTCCA GTCGGACCGT 
AGCCTCATCG CTGCGTTTTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG 
TCTGGGCTGC TGGTACTGCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAATGCC 
TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCCGGGA GCTGCCCTGT CCCCCAGGAG 
TGTGTGCCCC AGGATGGGGT CCCAGGTGCT GGACTCTGCC GGTGTCCCCA GGGCTGGGCT 
GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG 
ACGTGTAATC AGAGCCTGGG CGTGTGCGTC TGTGCTGAGG GCTTTGGGGG TCCTGACTGT 
GCCACGAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA 
GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT 
GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT 
AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCCGA GGATGGGGGC 
CCAGGCCCCT CCCCTCGCTC TTTCCACTCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC 
ATGTACCTGC TGGGGGGGCT CACAGCTGGG GGCATCGCCC GGGACTTCTG GGTCCTCAAC 
CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCCGTT 
GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC 
CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG 
TCCGGGGCTC AGAGCGGGAC ACCCCCCACA GGTCTCTACG GTCACTCTGC TGTGTATCAT 
GAGGCCACCG ACTCCCTCTA CGTGTTCGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC 
CCGTCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT 
TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCACGCCT CGGCCTTGCT AGGGGACACC 
ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGCGACGT GCTGCTCTAC 
CAGGTCAACT GCAACACCTG GCTCCTGCCC GACCTCATCG GCCCAGCCTC TGTGGGACCG 
CCAATGGAAG AGTCTGTGGC TCACGCCGTG GCCGCGGTAG GGGGTCACCT GTTTATCTCA 
GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCC TGACCCTGCC CCCTGACCCC 
TGCCGCCTGC TGCCCTCGCC CGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT 
GGAGCCTGCC TGTCTGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCAC CCCCTGCTCC 
CCTATGCCCC ACTCCCCCGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC 
CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCT CTGCCCCACG CTGTAAGTGG 
TGTACCAACT GCCCCGAGGG TGCCTGCATT GGGCGCAACG GGTCCTGCAC CTCAGAGAAT 
GACTGTCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCGGA GGCTGTGTGC 
GGGGCGGCCG ACTGCGAGCA GTGCACCAGG GAGGGCAAAT GCATGTGGAC GCGGCAGTTC 
AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC 
TTCAGCCACT CACTGCTGAA CGTGTCCCCG ATGCCAGTGG AGTCATCGCC CCCGCTGCCC 
TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT 
GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC 
TATCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC 
TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGTGCCCTGT GCCTGCGGCG CCCCCACTGT 
GGCTGGTGTA CCTGGGGGGG CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT 
GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CCTGGGCCTT TCTTTCCTGC 
CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ACGAGACCCA GAACTGCCAC 
GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG 
GGGCTGTGCC GCCCAGTGTG CGCCCAGGGT TGCGTGAACG GCTCGTGTGT GGAGCCCGAC 
CACTGCCGCT GCCACTTCGG CTTCGTCGGC CGCAACTGTT CCACGGAGTG TCGCTGCAAC 
CGCCACAGCG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAATCAC 
ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCGCTGTTCG TGGGCTCAGC CGTAGGGGGA 
GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGAAACA GCCACGTCTG CGTCTCCAGG 
AAGGAGCTTG AGATAGCCAG GAGGGAGCCG GAGAAGTACT CGCTGGATCC AGATGAGATT 
GAACACTGGG CGACAGAGGG TCCCAGCGAA GATGAGGCTG TGTGTGTGAA CTGCCAGAAT 
AACAGCTATG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTCCTCCT CGACGGGAAA 
TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACATGTA ATGAGCAGGA TGGGACAGGC 
TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC 
CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA 
GGGGGCCAGC AGTGCTACCG CCTCATCTCC GTGGAACAGG AGTACTGCCT GGACCCCACG 
TCCCAAACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGTCCCGG ACGCACCGTT 
CTCTTTGGAG TGCAGCCTAA GTTCACCAAC GTGGACATCC GCCTGACGCT GGACGTGACC 
TTTGGCGCCG TGGACCTCTA CGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT 
CCCGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CACCTCCCCC ACCCCCACCA 
CCCCCTGCTG ACGGCGGGCC CCGGGGACCT GGGGATCTCG GGGGACCTGG GGCTGGAGGT 
GGGCCGGCTA CCCCAGCAGA GCCGCGAGTG CGGGAGGTCT GGCCACGGGG CCTGATCACC 
TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG 
GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG 
CTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG 
CTGTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC TGTCTTCTTC 
TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG 
GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC 
TTCGCCAAGG TCACTGTCTG CTTCCCGCCA GACCCTGCTG CCCCAGCCCC CACCTGGAAG 
CCTGCCGGGC TCCCGCCACC CGCCTTCCGC CGCTCCGAGC CCTTCTTGGC ACCCTTGCTG 
CTGACCGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGGG GCTGCTGCCC ACCGGCCATC 
CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC 
ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGCG GGCCCCACGC ACCCAACGGC 
GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAATA CTGCGGCGGC 
GGCGGGGGCG CTGGGGGCAG TGGGCATGGG GCTGGTGCGG GCCGGAAGGG GCTGTTGAGC 
CAGGACAACC TCACCAGCAT GTCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015313724.1
CDS	1064..9193
Translation	MALGRALAVALAVSLAVLGPLSPGAQAGDCKGQRQVLREAPGFVTDGAGNYSVNGNCEWLIEAPSSQHRILLDFLFLDTECTYDYLFVYDGDSPRGPLLASLSGSTRPPPIEASSGKMLLHLFSDANYNLLGFNASFRFSLCPGGCRSHGQCQPPGVCVCEPGWGGPDCSLQECSAYCGSHGTCTSPLGPCRCEPGFLGRACDLHLWENQGAGWWHNVSAGDPAFSARIGAAGAFLSPLGLLAVFGGQDLNRALGDLVLYNFSANTWERWDLSPAPAARHSHVAVAWAGSLVLMGGELADGTLTSDIWAFSPMGGGRWELLAPPASSPSGPPGLAGHAAALVDNTWLYVSGGRTQHDLFSSGLFRFRLDGTSGGYWEQVMPAGGRPPAATGHSMVFHAASRALLVHGGHRPSTARFSVRVNSTELFHVDRRVWTTLKGREGLHGPRERAFHTASVLGNYMLVYGGNVHTHYQEEKCYEDGIFFYHLGCHQWVSGAELAPPGTPEGRAAPPSGRYSHVAAVLGGSVLLVAGGYSGRPRGDLMAYKVPPFVFQAPAPDYHLDYCSMYTDHSVCSRDPECSWCQGACQAAPPPGTPSGACPAASCLGLSRLLGDCQACLAFSSSAAPPQGPGALGWCVHNESCLPRPGKCPQHPVHDTRDSSGSGHSSGTEAPSARCGEAGVRPPELPPPRPTSTLPFRAPAARPHLSSQPRQPPCGYRASGAHWLQSGGHSSGLFRAPRPRPRRLHAASPLRLPGAHLGAGVAVAMLLAERAEGRHQHEQGPAGPSVPTVGAALAQVAGGAGQAPAAALVCQAAQAGGVGGAGRRAVGLQEEEGDLLGVRVSEEGEGSMLQVKPAECPRHSYHVFCVRAPGHPGQHMQALGPGPHGHILSPRPPRASRQQRVDEATSASLPFLLSVSIFIFVSLWFISSLPVPVSLSLSLLCPSTPIAFFNPTPRSCLQGDFSGPLSGGNCSLWVGEGLGLSVALPARWAYARCPDVDECRLGLARCHLRATCLNTPLSYECHCQRGYQGDGVTYCNRTCLEDCGHGVCSGPPDFTCVCDLGWTSDLPLPTPAPGPPAPRCSRDCGCNFHSHCHKRGPGFCDECQDWTWGEHCERCRPGSFGNATGSGGCRPCQCNGHGDPRRGHCDNLSGLCFCQDHTEGAHCQLCSPGYYGDPRAGGSCFRECGGRALLTNVSSVALGSRRFGGLLPAGGGAARAGPGLSYCVWVVSATEVLQPCAPGTLCPPLTLTFSPDSSTPCTLSYVLAFDGFPRFLDTGVVQSDRSLIAAFCGQRRDRPLTVQALSGLLVLHWEANGSSSWGFNASVGSARCGSGGPGSCPVPQECVPQDGVPGAGLCRCPQGWAGPHCRMAVCPENCNAHNGAGTCNQSLGVCVCAEGFGGPDCATKLDGGQLVWETLMDSRLSADTASRFLHRLGHTMVEGPDATLWMFGGLGLPQGLLGNLYRYSVSERRWTQMLAGAEDGGPGPSPRSFHSAAYVPAGRGAMYLLGGLTAGGIARDFWVLNLTTLQWRQEKAPQTVELPAVAGHTLTARRGLSLLLVGGYSPENGFNQQLLEYQLATGTWVSGAQSGTPPTGLYGHSAVYHEATDSLYVFGGFRFHVELAAPSPELYSLHCPDRTWSLLAPSQGAKPRPRLFHASALLGDTMVVLGGRSDPDEFSSDVLLYQVNCNTWLLPDLIGPASVGPPMEESVAHAVAAVGGHLFISGGFGGVALGRLLALTLPPDPCRLLPSPEACNQTGACTWCHGACLSGDQAHRLGCGGTPCSPMPHSPEECRRLRTCSECLARHPRTLQPGDGEASAPRCKWCTNCPEGACIGRNGSCTSENDCRINQREVFWAGNCSEAVCGAADCEQCTREGKCMWTRQFKRTGETRRILSVQPTYDWTCFSHSLLNVSPMPVESSPPLPCPTPCHLLPNCTSCLDSKGADGGWQHCVWSSSLQQCLSPSYLPLRCMAGGCGRLLRGPESCSLGCAQATQCALCLRRPHCGWCTWGGQDGGGRCLEGGLSGPRDGLTCGRPGASWAFLSCPPEDECANGHHDCNETQNCHDRPHGYECSCKAGYTMDNVTGLCRPVCAQGCVNGSCVEPDHCRCHFGFVGRNCSTECRCNRHSDCAGVGARDHCLLCHNHTKGSHCEQCLPLFVGSAVGGGTCRPCHAFCRGNSHVCVSRKELEIARREPEKYSLDPDEIEHWATEGPSEDEAVCVNCQNNSYGDKCESCLHGYFLLDGKCTKCQCNGHADTCNEQDGTGCPCQNNTETGTCQGSSPSDRRDCYKYQCAKCRESFHGSPLGGQQCYRLISVEQEYCLDPTSQTNCFHEPKRRALGPGRTVLFGVQPKFTNVDIRLTLDVTFGAVDLYVSTSYDTFVVRVAPDTGVHTVHIQPPPPPPPPPPPADGGPRGPGDLGGPGAGGGPATPAEPRVREVWPRGLITYVTVTEPSAVLVVRGVRDRLVITYPHEHHTLKSSRFYLLLLGVGDPSGPSANGSADSQGLLFFRQDQAHIDLFVFFSVFFSCFFLFLSLCVLLWKAKQALDHRQEQRRHLQEMTKMASRPFAKVTVCFPPDPAAPAPTWKPAGLPPPAFRRSEPFLAPLLLTGAGGPWGPVGGGCCPPAIPTTTAGLRAGPITLEPTEDGMAGVATLLLQLPGGPHAPNGACLGSALVTLRHRLHEYCGGGGGAGGSGHGAGAGRKGLLSQDNLTSMSL

Target ORF information:

RefSeq Version	XM_015458238.1
Organism	Bos taurus(cattle)
Definition	Bos taurus multiple EGF like domains 8 (MEGF8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458238.1

ORF Insert Sequence:

ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTGTAT CATTGGCCGT GCTGGGGCCA 
CTGTCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT 
CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC 
ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA 
TGCACTTATG ACTACCTGTT CGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC 
AGTCTGAGTG GGAGCACACG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTG 
CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC 
CTGTGCCCGG GTGGCTGCCG CAGCCATGGG CAGTGCCAGC CCCCAGGGGT GTGTGTCTGT 
GAGCCGGGCT GGGGGGGCCC CGACTGCAGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC 
CATGGCACCT GCACCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGC 
GCCTGTGACC TGCACCTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC 
GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACTGGGG 
CTGCTGGCTG TTTTTGGAGG CCAGGACCTC AACCGCGCGC TGGGGGACCT CGTCCTGTAC 
AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC 
TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT 
GGCACCCTCA CCAGCGACAT CTGGGCCTTT AGCCCGATGG GTGGCGGCCG CTGGGAGCTT 
CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA TGCAGCAGCC 
CTGGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC 
TCCGGCCTCT TCCGTTTCCG CCTCGACGGC ACCAGTGGGG GCTATTGGGA GCAGGTGATG 
CCAGCAGGTG GGCGGCCCCC TGCCGCCACC GGCCATTCCA TGGTGTTCCA TGCCGCCTCC 
CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG 
AACTCCACCG AGCTCTTCCA TGTGGATCGG CGCGTGTGGA CCACCCTGAA GGGCCGGGAG 
GGACTTCACG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG 
CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC 
ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCTGAGCT TGCCCCTCCA 
GGAACTCCAG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG 
CTTGGTGGCA GTGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCTCG TGGGGACTTG 
ATGGCCTACA AAGTGCCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGATTA CCACTTGGAC 
TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGTTGGTGT 
CAGGGGGCCT GCCAAGCCGC CCCACCTCCT GGGACTCCCT CAGGGGCTTG TCCGGCTGCC 
AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC 
AGTGCGGCTC CCCCCCAGGG GCCCGGTGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC 
CTCCCTCGGC CTGGTAAGTG TCCACAGCAC CCCGTGCACG ACACTCGTGA CAGCTCGGGA 
AGCGGTCACA GCTCCGGCAC CGAGGCTCCG AGTGCACGCT GTGGGGAGGC GGGTGTGAGG 
CCGCCCGAGC TACCCCCGCC CCGCCCCACC TCCACCCTCC CCTTCCGCGC CCCTGCCGCC 
CGCCCGCACC TGTCGTCCCA GCCTCGGCAG CCCCCGTGCG GGTACCGGGC CAGCGGAGCT 
CACTGGCTGC AGAGTGGGGG GCACTCTTCC GGACTCTTCA GGGCGCCCCG ACCCCGGCCG 
CGCCGGCTGC ACGCCGCGTC CCCCTTGCGG CTGCCTGGTG CGCACCTGGG TGCAGGCGTG 
GCAGTCGCGA TGCTCCTCGC AGAGCGGGCA GAGGGCAGGC ACCAGCACGA GCAGGGACCC 
GCCGGCCCCA GCGTCCCTAC AGTGGGCGCC GCTCTGGCGC AGGTGGCAGG TGGCGCTGGC 
CAGGCACCAG CCGCAGCCCT GGTCTGCCAG GCAGCCCAGG CAGGAGGAGT AGGAGGCGCA 
GGCCGACGAG CGGTAGGGCT CCAGGAAGAA GAAGGAGATC TCCTGGGGGT GAGGGTGTCA 
GAGGAGGGTG AGGGCTCCAT GCTCCAGGTC AAGCCTGCTG AGTGTCCCAG GCATTCTTAC 
CATGTTTTCT GTGTCCGGGC CCCGGGCCAT CCGGGCCAGC ACATGCAGGC GCTGGGCCCG 
GGCCCACACG GCCACATCCT CAGCCCCAGA CCCCCCAGGG CCTCCCGGCA GCAGCGGGTG 
GATGAAGCCA CGTCTGCCTC TCTTCCATTT CTCCTGTCTG TCTCCATATT CATCTTTGTG 
TCTCTCTGGT TTATCTCCTC TCTCCCTGTC CCCGTCTCTC TGTCTCTCTC CTTACTTTGC 
CCCTCCACCC CAATCGCCTT CTTCAACCCC ACTCCCCGCA GTTGCCTCCA AGGGGACTTC 
TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT 
GTGGCCCTCC CCGCCCGCTG GGCGTATGCC CGCTGTCCGG ATGTAGACGA GTGCCGCCTG 
GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTACGAGTGT 
CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG 
GACTGTGGCC ACGGTGTGTG CAGCGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC 
TGGACATCAG ACCTGCCCCT TCCCACGCCG GCCCCCGGCC CCCCCGCCCC CCGCTGTTCG 
CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAT 
GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCTGG AAGCTTCGGG 
AATGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ACGGGCATGG GGACCCTCGC 
CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC 
CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC 
CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC 
CGGTTTGGGG GGCTCCTGCC TGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC 
TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTGCAGC CCTGTGCCCC CGGGACCCTC 
TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAT 
GTCCTGGCCT TTGATGGATT CCCACGCTTC CTAGACACTG GTGTCGTCCA GTCGGACCGT 
AGCCTCATCG CTGCGTTTTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG 
TCTGGGCTGC TGGTACTGCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAATGCC 
TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCCGGGA GCTGCCCTGT CCCCCAGGAG 
TGTGTGCCCC AGGATGGGGT CCCAGGTGCT GGACTCTGCC GGTGTCCCCA GGGCTGGGCT 
GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG 
ACGTGTAATC AGAGCCTGGG CGTGTGCGTC TGTGCTGAGG GCTTTGGGGG TCCTGACTGT 
GCCACGAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA 
GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT 
GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT 
AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCCGA GGATGGGGGC 
CCAGGCCCCT CCCCTCGCTC TTTCCACTCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC 
ATGTACCTGC TGGGGGGGCT CACAGCTGGG GGCATCGCCC GGGACTTCTG GGTCCTCAAC 
CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCCGTT 
GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC 
CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG 
TCCGGGGCTC AGAGCGGGAC ACCCCCCACA GGTCTCTACG GTCACTCTGC TGTGTATCAT 
GAGGCCACCG ACTCCCTCTA CGTGTTCGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC 
CCGTCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT 
TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCACGCCT CGGCCTTGCT AGGGGACACC 
ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGCGACGT GCTGCTCTAC 
CAGGTCAACT GCAACACCTG GCTCCTGCCC GACCTCATCG GCCCAGCCTC TGTGGGACCG 
CCAATGGAAG AGTCTGTGGC TCACGCCGTG GCCGCGGTAG GGGGTCACCT GTTTATCTCA 
GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCC TGACCCTGCC CCCTGACCCC 
TGCCGCCTGC TGCCCTCGCC CGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT 
GGAGCCTGCC TGTCTGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCAC CCCCTGCTCC 
CCTATGCCCC ACTCCCCCGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC 
CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCT CTGCCCCACG CTGTAAGTGG 
TGTACCAACT GCCCCGAGGG TGCCTGCATT GGGCGCAACG GGTCCTGCAC CTCAGAGAAT 
GACTGTCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCGGA GGCTGTGTGC 
GGGGCGGCCG ACTGCGAGCA GTGCACCAGG GAGGGCAAAT GCATGTGGAC GCGGCAGTTC 
AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC 
TTCAGCCACT CACTGCTGAA CGTGTCCCCG ATGCCAGTGG AGTCATCGCC CCCGCTGCCC 
TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT 
GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC 
TATCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC 
TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGTGCCCTGT GCCTGCGGCG CCCCCACTGT 
GGCTGGTGTA CCTGGGGGGG CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT 
GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CCTGGGCCTT TCTTTCCTGC 
CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ACGAGACCCA GAACTGCCAC 
GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG 
GGGCTGTGCC GCCCAGTGTG CGCCCAGGGT TGCGTGAACG GCTCGTGTGT GGAGCCCGAC 
CACTGCCGCT GCCACTTCGG CTTCGTCGGC CGCAACTGTT CCACGGAGTG TCGCTGCAAC 
CGCCACAGCG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAATCAC 
ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCGCTGTTCG TGGGCTCAGC CGTAGGGGGA 
GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGAAACA GCCACGTCTG CGTCTCCAGG 
AAGGAGCTTG AGATAGCCAG GAGGGAGCCG GAGAAGTACT CGCTGGATCC AGATGAGATT 
GAACACTGGG CGACAGAGGG TCCCAGCGAA GATGAGGCTG TGTGTGTGAA CTGCCAGAAT 
AACAGCTATG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTCCTCCT CGACGGGAAA 
TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACATGTA ATGAGCAGGA TGGGACAGGC 
TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC 
CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA 
GGGGGCCAGC AGTGCTACCG CCTCATCTCC GTGGAACAGG AGTACTGCCT GGACCCCACG 
TCCCAAACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGTCCCGG ACGCACCGTT 
CTCTTTGGAG TGCAGCCTAA GTTCACCAAC GTGGACATCC GCCTGACGCT GGACGTGACC 
TTTGGCGCCG TGGACCTCTA CGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT 
CCCGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CACCTCCCCC ACCCCCACCA 
CCCCCTGCTG ACGGCGGGCC CCGGGGACCT GGGGATCTCG GGGGACCTGG GGCTGGAGGT 
GGGCCGGCTA CCCCAGCAGA GCCGCGAGTG CGGGAGGTCT GGCCACGGGG CCTGATCACC 
TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG 
GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG 
CTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG 
CTGTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC TGTCTTCTTC 
TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG 
GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC 
TTCGCCAAGG TCACTGTCTG CTTCCCGCCA GACCCTGCTG CCCCAGCCCC CACCTGGAAG 
CCTGCCGGGC TCCCGCCACC CGCCTTCCGC CGCTCCGAGC CCTTCTTGGC ACCCTTGCTG 
CTGACCGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGGG GCTGCTGCCC ACCGGCCATC 
CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC 
ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGCG GGCCCCACGC ACCCAACGGC 
GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAATA CTGCGGCGGC 
GGCGGGGGCG CTGGGGGCAG TGGGCATGGG GCTGGTGCGG GCCGGAAGGG GCTGTTGAGC 
CAGGACAACC TCACCAGCAT GTCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PHONE

1-877-436-7274

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MEGF8 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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