TRMT1L cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRMT1L
Entrez Gene ID	540872
Full Name	tRNA methyltransferase 1 like
Synonyms	TRM1L
General protein information	Preferred Names tRNA methyltransferase 1 like Names TRMT1-like protein TRM1 tRNA methyltransferase 1-like TRM1-like protein tRNA methyltransferase 1 homolog-like
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024976424.1	XP_024832192.1	TRMT1-like protein isoform X2
XM_005217380.3	XP_005217437.1	TRMT1-like protein isoform X1
XM_005217380.4	XP_005217437.2	TRMT1-like protein isoform X1
NM_001098121.1	NP_001091590.1	TRMT1-like protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRMT1L	NP_112196.3
Canis lupus familiaris (dog)	TRMT1L	XP_849853.1
Rattus norvegicus (Norway rat)	Trmt1l	NP_001032269.1
Pan troglodytes (chimpanzee)	TRMT1L	XP_514058.2
Macaca mulatta (Rhesus monkey)	TRMT1L	XP_001113820.1
Xenopus tropicalis (tropical clawed frog)	trmt1l	XP_004913842.1
Gallus gallus (chicken)	TRMT1L	XP_003643222.2
Danio rerio (zebrafish)	trmt1l	XP_688031.4
Bos taurus (cattle)	TRMT1L	NP_001091590.1
Mus musculus (house mouse)	Trmt1l	NP_081152.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TRMT1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRMT1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43676	NM_001098121.1 Latest version!	Bos taurus tRNA methyltransferase 1 like (TRMT1L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa223706	XM_005217380.4 Latest version!	Bos taurus tRNA methyltransferase 1 like (TRMT1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa223707	XM_024976424.1 Latest version!	Bos taurus tRNA methyltransferase 1 like (TRMT1L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa27532	XM_005217380.3 Latest version!	Bos taurus tRNA methyltransferase 1 like (TRMT1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRMT1L ( NM_001098121.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001091590.1 Bos taurus tRNA methyltransferase 1 like (TRMT1L), mRNA.

Price & Availability↑

CloneID	OBa43676
Clone ID Related Accession (Same CDS sequence)	NM_001098121.1
Accession Version	NM_001098121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Bos taurus(cattle)
Product	TRMT1-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC140663.1. On May 24, 2007 this sequence version replaced XM_613543.3. ##Evidence-Data-START## Transcript exon combination :: BC140663.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCAGGAGA AGGTGGAGGT GGTCCAGGTC 
CCGGTCCCGA CCCCGACCCC GGACTCGGCT CGGGTCCCAG CACCGGCCCC GGATTCGGCT 
CCGGTCTCGG CCTCGACCCC GGCCCCGGCC TCGGCTCCGA CTCCGGCCTC GGCTCCAGTC 
CCAGCCCCTG CCCTGGCCCA GGCTTCGGCC CTGTCCCCGT CCCTAGCCTC TGCCCCTGAT 
GAGGCTGAAA GCAAGAGACA CATCTCAATT CAAAGGCAGC TTGCTGATTT AGAGAAGTTA 
GCTTTCGTAA CTGAAGGGGA TTGTGACTCT GCCAATTCAC TGAACTCAGA TAATCTTGAT 
GCAGGAAACA AACAGGCTTG TCCATTGTGC CCCAAGGAGA AATTCAGAGC TTGTAATAGT 
CACAAGCTTC ATCGTCACCT TCAGAATTTA CACTGGAAAG TCTCCGTCGA ATTTGAAGGT 
TACCGGATGT GCATCTGTCA CTTACCTTGT CGACCGGTGA AACCAAACAT TATTGGAGAA 
CAGATATCCA GTAAAATGGG CGCCCATTAT CATTGTATCA TTTGTTCAGC AACAATCACC 
AGAAGGACAG ATATGCTAGG ACATGTCAGG CGCCATGTGA ACAAAGGAGA GACTAAATCC 
AGGTATATTG CTGCTTCTGC TGCTAAACCA CCTAAAGAAA TTTTGAAAGA GGCAGACACA 
GATGTACAAG TTTGTCCTAA CTATACTGTA CCTCAGAAAA CAGATTCTTA TTTTAATCCC 
AAAATGAAAC TGAATCGGCA GCTGATATTC TGTACACTGG CTGCTCTGGC TAAGGAGCGA 
AAACCTTTGG AATGTCTAGA TGCCTTTGGA GCAACTGGGA TAATGGGATT ACAATGGGCA 
AAACATCTTG GAAATGCAGT CAAGGTTACA ATTAATGATT TGAATGAAAA CTCTGTGACG 
TTGATTCAGG AGAACTGCCA TTTAAACAAA TTGAAAGTGG TTGTGGACAG TAAGGAAAAG 
GAAGAAAGGG AAGATATTCT GGAAGAAGGA GAGGAAAACC TTGGTAATAT TAAGGTGACC 
AAAATGGATG CCAATGTACT AATGCATTTG AGATCTTTTG ATTTCATACA CCTAGATCCT 
TTTGGAACAT CTGTGAACTA TCTTGATTCA GCATTCAGAA ATATAAGAAA CCTTGGCATA 
GTGTCAGTGA CTTCTACAGA TATCAGTTCT TTATATGCCA AGGCACAACA CGTTGCCCGG 
CGTCACTACG GCTGTAATAT TGTCCGAACT GAATATTACA AGGAGCTAGC TGCAAGAATT 
GTTGTAGCTG CAGTGGCAAG AGCTGCAGCC CGATGTAACA AAGGCATAGA AGTACTATTT 
GCGGTGGCTC TGGAACATTT TGTGTTGGTT GTTGTGAGAG TTTTGAGGGG GCCAACTTCT 
GCAGATGAGA CAGCCAAGAA GATTCAGTAC CTAATCCATT GTCAGTGGTG TGAAGAGAGA 
ATTTTTCAGA AGGATGGTAA TATGGTAGAA GAAAACCCAT ATAGACAGCT ACCCTGTAAT 
TGTCATGGAA GCATGCCTGG AAAGACAGCA ATAGAACTTG GACCTCTATG GTCAAGTTCC 
CTTTTCAATA CTGGATTCCT CAAAAGAATG CTGTCTGAAT CTCTTCACCA TGGTTTAGAT 
GACATTCAGA CCCTAATAAA GACATTAATC TTTGAGTCAG AGTGTACTCC TCAGTTTTCA 
GTTCATGCAC CTTCAAATCT CAACAAGCCA GAAGAATGCG GTGTATTTAT TAAAACTACA 
GACGATACCA CAACAGATAG TCACAGCGCA CAAGGAAAGA GAAAAAGTAA TGAAACAACT 
GCAAATTTAG TCAAGAGGCA AAAGACTGAT GTCAATACTG AACATCCTCC CTTTTATTAC 
AACATCCACA GACACAGCAT TAAAGGAATG AATATGCCAA AGTTAAAAAA GTTTTTGTGC 
TATCTTTCTC AAGCAGGCTT TCGAGTAAGC CGAACTCATT TTGACCCAAT GGGAGTTCGT 
ACAGATGCAC CTCTAATGCA GTTTAAATCC ATCCTTTTGA AGTACAGCAC CCCTACTTAC 
ACCGGAGGAC AGTCAGAGGG CCATGTCCAA CCAGCATCTG AAGACACAGT AGCCGACAGG 
GTTGAAATGT CAGTGAATGA CAAAGCAGAA GCAGGTGGTT GCAGAAGATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091590.1
CDS	349..2562
Translation	MENMAEEELLPQEKVEVVQVPVPTPTPDSARVPAPAPDSAPVSASTPAPASAPTPASAPVPAPALAQASALSPSLASAPDEAESKRHISIQRQLADLEKLAFVTEGDCDSANSLNSDNLDAGNKQACPLCPKEKFRACNSHKLHRHLQNLHWKVSVEFEGYRMCICHLPCRPVKPNIIGEQISSKMGAHYHCIICSATITRRTDMLGHVRRHVNKGETKSRYIAASAAKPPKEILKEADTDVQVCPNYTVPQKTDSYFNPKMKLNRQLIFCTLAALAKERKPLECLDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEEREDILEEGEENLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEENPYRQLPCNCHGSMPGKTAIELGPLWSSSLFNTGFLKRMLSESLHHGLDDIQTLIKTLIFESECTPQFSVHAPSNLNKPEECGVFIKTTDDTTTDSHSAQGKRKSNETTANLVKRQKTDVNTEHPPFYYNIHRHSIKGMNMPKLKKFLCYLSQAGFRVSRTHFDPMGVRTDAPLMQFKSILLKYSTPTYTGGQSEGHVQPASEDTVADRVEMSVNDKAEAGGCRRW

Target ORF information:

RefSeq Version	NM_001098121.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 1 like (TRMT1L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098121.1

ORF Insert Sequence:

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCAGGAGA AGGTGGAGGT GGTCCAGGTC 
CCGGTCCCGA CCCCGACCCC GGACTCGGCT CGGGTCCCAG CACCGGCCCC GGATTCGGCT 
CCGGTCTCGG CCTCGACCCC GGCCCCGGCC TCGGCTCCGA CTCCGGCCTC GGCTCCAGTC 
CCAGCCCCTG CCCTGGCCCA GGCTTCGGCC CTGTCCCCGT CCCTAGCCTC TGCCCCTGAT 
GAGGCTGAAA GCAAGAGACA CATCTCAATT CAAAGGCAGC TTGCTGATTT AGAGAAGTTA 
GCTTTCGTAA CTGAAGGGGA TTGTGACTCT GCCAATTCAC TGAACTCAGA TAATCTTGAT 
GCAGGAAACA AACAGGCTTG TCCATTGTGC CCCAAGGAGA AATTCAGAGC TTGTAATAGT 
CACAAGCTTC ATCGTCACCT TCAGAATTTA CACTGGAAAG TCTCCGTCGA ATTTGAAGGT 
TACCGGATGT GCATCTGTCA CTTACCTTGT CGACCGGTGA AACCAAACAT TATTGGAGAA 
CAGATATCCA GTAAAATGGG CGCCCATTAT CATTGTATCA TTTGTTCAGC AACAATCACC 
AGAAGGACAG ATATGCTAGG ACATGTCAGG CGCCATGTGA ACAAAGGAGA GACTAAATCC 
AGGTATATTG CTGCTTCTGC TGCTAAACCA CCTAAAGAAA TTTTGAAAGA GGCAGACACA 
GATGTACAAG TTTGTCCTAA CTATACTGTA CCTCAGAAAA CAGATTCTTA TTTTAATCCC 
AAAATGAAAC TGAATCGGCA GCTGATATTC TGTACACTGG CTGCTCTGGC TAAGGAGCGA 
AAACCTTTGG AATGTCTAGA TGCCTTTGGA GCAACTGGGA TAATGGGATT ACAATGGGCA 
AAACATCTTG GAAATGCAGT CAAGGTTACA ATTAATGATT TGAATGAAAA CTCTGTGACG 
TTGATTCAGG AGAACTGCCA TTTAAACAAA TTGAAAGTGG TTGTGGACAG TAAGGAAAAG 
GAAGAAAGGG AAGATATTCT GGAAGAAGGA GAGGAAAACC TTGGTAATAT TAAGGTGACC 
AAAATGGATG CCAATGTACT AATGCATTTG AGATCTTTTG ATTTCATACA CCTAGATCCT 
TTTGGAACAT CTGTGAACTA TCTTGATTCA GCATTCAGAA ATATAAGAAA CCTTGGCATA 
GTGTCAGTGA CTTCTACAGA TATCAGTTCT TTATATGCCA AGGCACAACA CGTTGCCCGG 
CGTCACTACG GCTGTAATAT TGTCCGAACT GAATATTACA AGGAGCTAGC TGCAAGAATT 
GTTGTAGCTG CAGTGGCAAG AGCTGCAGCC CGATGTAACA AAGGCATAGA AGTACTATTT 
GCGGTGGCTC TGGAACATTT TGTGTTGGTT GTTGTGAGAG TTTTGAGGGG GCCAACTTCT 
GCAGATGAGA CAGCCAAGAA GATTCAGTAC CTAATCCATT GTCAGTGGTG TGAAGAGAGA 
ATTTTTCAGA AGGATGGTAA TATGGTAGAA GAAAACCCAT ATAGACAGCT ACCCTGTAAT 
TGTCATGGAA GCATGCCTGG AAAGACAGCA ATAGAACTTG GACCTCTATG GTCAAGTTCC 
CTTTTCAATA CTGGATTCCT CAAAAGAATG CTGTCTGAAT CTCTTCACCA TGGTTTAGAT 
GACATTCAGA CCCTAATAAA GACATTAATC TTTGAGTCAG AGTGTACTCC TCAGTTTTCA 
GTTCATGCAC CTTCAAATCT CAACAAGCCA GAAGAATGCG GTGTATTTAT TAAAACTACA 
GACGATACCA CAACAGATAG TCACAGCGCA CAAGGAAAGA GAAAAAGTAA TGAAACAACT 
GCAAATTTAG TCAAGAGGCA AAAGACTGAT GTCAATACTG AACATCCTCC CTTTTATTAC 
AACATCCACA GACACAGCAT TAAAGGAATG AATATGCCAA AGTTAAAAAA GTTTTTGTGC 
TATCTTTCTC AAGCAGGCTT TCGAGTAAGC CGAACTCATT TTGACCCAAT GGGAGTTCGT 
ACAGATGCAC CTCTAATGCA GTTTAAATCC ATCCTTTTGA AGTACAGCAC CCCTACTTAC 
ACCGGAGGAC AGTCAGAGGG CCATGTCCAA CCAGCATCTG AAGACACAGT AGCCGACAGG 
GTTGAAATGT CAGTGAATGA CAAAGCAGAA GCAGGTGGTT GCAGAAGATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1L ( XM_005217380.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217437.2 Bos taurus tRNA methyltransferase 1 like (TRMT1L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa223706
Clone ID Related Accession (Same CDS sequence)	XM_005217380.4
Accession Version	XM_005217380.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	TRMT1-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217380.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCAGGAGA AGGTGGAGGT GGTCCAGGTC 
CCGGTCCCGA CCCCGACCCC GGACTCGGCT CGGGTCCCAG CACCGGCCCC GGATTCGGCT 
CCGGTCTCGG CCTCGACCCC GGCCCCGGCC TCGGCTCCGA CTCCGGCCTC GGCTCCAGTC 
CCAGCCCCTG CCCTGGCCCA GGCTTCGGCC CTGTCCCCGT CCCTAGCCTC TGCCCCTGAT 
GAGGCTGAAA GCAATGGTCT AGTTAGCCCT TTGAAATCAG TCTCATATAG GCAAGATGAA 
GATGGCATAT CTGAAGTAGA ATTGAAAATT GAGGAAGAAA AAGAAGTTTT AAAAGAACCG 
CCTAGCATCT TGGAATCATC TGATTTAATA AGAGAGAGAC ACATCTCAAT TCAAAGGCAG 
CTTGCTGATT TAGAGAAGTT AGCTTTCGTA ACTGAAGGGG ATTGTGACTC TGCCAATTCA 
CTGAACTCAG ATAATCTTGA TGCAGGAAAC AAACAGGCTT GTCCATTGTG CCCCAAGGAG 
AAATTCAGAG CTTGTAATAG TCACAAGCTT CATCGTCACC TTCAGAATTT ACACTGGAAA 
GTCTCCGTCG AATTTGAAGG TTACCGGATG TGCATCTGTC ACTTACCTTG TCGACCGGTG 
AAACCAAACA TTATTGGAGA ACAGATATCC AGTAAAATGG GCGCCCATTA TCATTGTATC 
ATTTGTTCAG CAACAATCAC CAGAAGGACA GATATGCTAG GACATGTCAG GCGCCATGTG 
AACAAAGGAG AGACTAAATC CAGGTATATT GCTGCTTCTG CTGCTAAACC ACCTAAAGAA 
ATTTTGAAAG AGGCAGACAC AGATGTACAA GTTTGTCCTA ACTATACTGT ACCTCAGAAA 
ACAGATTCTT ATTTTAATCC CAAAATGAAA CTGAATCGGC AGCTGATATT CTGTACACTG 
GCTGCTCTGG CTAAGGAGCG AAAACCTTTG GAATGTCTAG ATGCCTTTGG AGCAACTGGG 
ATAATGGGAT TACAATGGGC AAAACATCTT GGAAATGCAG TCAAGGTTAC AATTAATGAT 
TTGAATGAAA ACTCTGTGAC GTTGATTCAG GAGAACTGCC ATTTAAACAA ATTGAAAGTG 
GTTGTGGACA GTAAGGAAAA GGAAGAAAGG GAAGATATTC TGGAAGAAGG AGAGGAAAAC 
CTTGGTAATA TTAAGGTGAC CAAAATGGAT GCCAATGTAC TAATGCATTT GAGATCTTTT 
GATTTCATAC ACCTAGATCC TTTTGGAACA TCTGTGAACT ATCTTGATTC AGCATTCAGA 
AATATAAGAA ACCTTGGCAT AGTGTCAGTG ACTTCTACAG ATATCAGTTC TTTATATGCC 
AAGGCACAAC ACGTTGCCCG GCGTCACTAC GGCTGTAATA TTGTCCGAAC TGAATATTAC 
AAGGAGCTAG CTGCAAGAAT TGTTGTAGCT GCAGTGGCAA GAGCTGCAGC CCGATGTAAC 
AAAGGCATAG AAGTACTATT TGCGGTGGCT CTGGAACATT TTGTGTTGGT TGTTGTGAGA 
GTTTTGAGGG GGCCAACTTC TGCAGATGAG ACAGCCAAGA AGATTCAGTA CCTAATCCAT 
TGTCAGTGGT GTGAAGAGAG AATTTTTCAG AAGGATGGTA ATATGGTAGA AGAAAACCCA 
TATAGACAGC TACCCTGTAA TTGTCATGGA AGCATGCCTG GAAAGACAGC AATAGAACTT 
GGACCTCTAT GGTCAAGTTC CCTTTTCAAT ACTGGATTCC TCAAAAGAAT GCTGTCTGAA 
TCTCTTCACC ATGGTTTAGA TGACATTCAG ACCCTAATAA AGACATTAAT CTTTGAGTCA 
GAGTGTACTC CTCAGTTTTC AGTTCATGCA CCTTCAAATC TCAACAAGCC AGAAGAATGC 
GGTGTATTTA TTAAAACTAC AGACGATACC ACAACAGATA GTCACAGCGC ACAAGGAAAG 
AGAAAAAGTA ATGAAACAAC TGCAAATTTA GTCAAGAGGC AAAAGACTGA TGTCAATACT 
GAACATCCTC CCTTTTATTA CAACATCCAC AGACACAGCA TTAAAGGAAT GAATATGCCA 
AAGTTAAAAA AGTTTTTGTG CTATCTTTCT CAAGCAGGCT TTCGAGTAAG CCGAACTCAT 
TTTGACCCAA TGGGAGTTCG TACAGATGCA CCTCTAATGC AGTTTAAATC CATCCTTTTG 
AAGTACAGCA CCCCTACTTA CACCGGAGGA CAGTCAGAGG GCCATGTCCA ACCAGCATCT 
GAAGACACAG TAGCCGACAG GGTTGAAATG TCAGTGAATG ACAAAGCAGA AGCAGGTGGT 
TGCAGAAGAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217437.2
CDS	343..2697
Translation	MENMAEEELLPQEKVEVVQVPVPTPTPDSARVPAPAPDSAPVSASTPAPASAPTPASAPVPAPALAQASALSPSLASAPDEAESNGLVSPLKSVSYRQDEDGISEVELKIEEEKEVLKEPPSILESSDLIRERHISIQRQLADLEKLAFVTEGDCDSANSLNSDNLDAGNKQACPLCPKEKFRACNSHKLHRHLQNLHWKVSVEFEGYRMCICHLPCRPVKPNIIGEQISSKMGAHYHCIICSATITRRTDMLGHVRRHVNKGETKSRYIAASAAKPPKEILKEADTDVQVCPNYTVPQKTDSYFNPKMKLNRQLIFCTLAALAKERKPLECLDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEEREDILEEGEENLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEENPYRQLPCNCHGSMPGKTAIELGPLWSSSLFNTGFLKRMLSESLHHGLDDIQTLIKTLIFESECTPQFSVHAPSNLNKPEECGVFIKTTDDTTTDSHSAQGKRKSNETTANLVKRQKTDVNTEHPPFYYNIHRHSIKGMNMPKLKKFLCYLSQAGFRVSRTHFDPMGVRTDAPLMQFKSILLKYSTPTYTGGQSEGHVQPASEDTVADRVEMSVNDKAEAGGCRRW

Target ORF information:

RefSeq Version	XM_005217380.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 1 like (TRMT1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217380.4

ORF Insert Sequence:

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCAGGAGA AGGTGGAGGT GGTCCAGGTC 
CCGGTCCCGA CCCCGACCCC GGACTCGGCT CGGGTCCCAG CACCGGCCCC GGATTCGGCT 
CCGGTCTCGG CCTCGACCCC GGCCCCGGCC TCGGCTCCGA CTCCGGCCTC GGCTCCAGTC 
CCAGCCCCTG CCCTGGCCCA GGCTTCGGCC CTGTCCCCGT CCCTAGCCTC TGCCCCTGAT 
GAGGCTGAAA GCAATGGTCT AGTTAGCCCT TTGAAATCAG TCTCATATAG GCAAGATGAA 
GATGGCATAT CTGAAGTAGA ATTGAAAATT GAGGAAGAAA AAGAAGTTTT AAAAGAACCG 
CCTAGCATCT TGGAATCATC TGATTTAATA AGAGAGAGAC ACATCTCAAT TCAAAGGCAG 
CTTGCTGATT TAGAGAAGTT AGCTTTCGTA ACTGAAGGGG ATTGTGACTC TGCCAATTCA 
CTGAACTCAG ATAATCTTGA TGCAGGAAAC AAACAGGCTT GTCCATTGTG CCCCAAGGAG 
AAATTCAGAG CTTGTAATAG TCACAAGCTT CATCGTCACC TTCAGAATTT ACACTGGAAA 
GTCTCCGTCG AATTTGAAGG TTACCGGATG TGCATCTGTC ACTTACCTTG TCGACCGGTG 
AAACCAAACA TTATTGGAGA ACAGATATCC AGTAAAATGG GCGCCCATTA TCATTGTATC 
ATTTGTTCAG CAACAATCAC CAGAAGGACA GATATGCTAG GACATGTCAG GCGCCATGTG 
AACAAAGGAG AGACTAAATC CAGGTATATT GCTGCTTCTG CTGCTAAACC ACCTAAAGAA 
ATTTTGAAAG AGGCAGACAC AGATGTACAA GTTTGTCCTA ACTATACTGT ACCTCAGAAA 
ACAGATTCTT ATTTTAATCC CAAAATGAAA CTGAATCGGC AGCTGATATT CTGTACACTG 
GCTGCTCTGG CTAAGGAGCG AAAACCTTTG GAATGTCTAG ATGCCTTTGG AGCAACTGGG 
ATAATGGGAT TACAATGGGC AAAACATCTT GGAAATGCAG TCAAGGTTAC AATTAATGAT 
TTGAATGAAA ACTCTGTGAC GTTGATTCAG GAGAACTGCC ATTTAAACAA ATTGAAAGTG 
GTTGTGGACA GTAAGGAAAA GGAAGAAAGG GAAGATATTC TGGAAGAAGG AGAGGAAAAC 
CTTGGTAATA TTAAGGTGAC CAAAATGGAT GCCAATGTAC TAATGCATTT GAGATCTTTT 
GATTTCATAC ACCTAGATCC TTTTGGAACA TCTGTGAACT ATCTTGATTC AGCATTCAGA 
AATATAAGAA ACCTTGGCAT AGTGTCAGTG ACTTCTACAG ATATCAGTTC TTTATATGCC 
AAGGCACAAC ACGTTGCCCG GCGTCACTAC GGCTGTAATA TTGTCCGAAC TGAATATTAC 
AAGGAGCTAG CTGCAAGAAT TGTTGTAGCT GCAGTGGCAA GAGCTGCAGC CCGATGTAAC 
AAAGGCATAG AAGTACTATT TGCGGTGGCT CTGGAACATT TTGTGTTGGT TGTTGTGAGA 
GTTTTGAGGG GGCCAACTTC TGCAGATGAG ACAGCCAAGA AGATTCAGTA CCTAATCCAT 
TGTCAGTGGT GTGAAGAGAG AATTTTTCAG AAGGATGGTA ATATGGTAGA AGAAAACCCA 
TATAGACAGC TACCCTGTAA TTGTCATGGA AGCATGCCTG GAAAGACAGC AATAGAACTT 
GGACCTCTAT GGTCAAGTTC CCTTTTCAAT ACTGGATTCC TCAAAAGAAT GCTGTCTGAA 
TCTCTTCACC ATGGTTTAGA TGACATTCAG ACCCTAATAA AGACATTAAT CTTTGAGTCA 
GAGTGTACTC CTCAGTTTTC AGTTCATGCA CCTTCAAATC TCAACAAGCC AGAAGAATGC 
GGTGTATTTA TTAAAACTAC AGACGATACC ACAACAGATA GTCACAGCGC ACAAGGAAAG 
AGAAAAAGTA ATGAAACAAC TGCAAATTTA GTCAAGAGGC AAAAGACTGA TGTCAATACT 
GAACATCCTC CCTTTTATTA CAACATCCAC AGACACAGCA TTAAAGGAAT GAATATGCCA 
AAGTTAAAAA AGTTTTTGTG CTATCTTTCT CAAGCAGGCT TTCGAGTAAG CCGAACTCAT 
TTTGACCCAA TGGGAGTTCG TACAGATGCA CCTCTAATGC AGTTTAAATC CATCCTTTTG 
AAGTACAGCA CCCCTACTTA CACCGGAGGA CAGTCAGAGG GCCATGTCCA ACCAGCATCT 
GAAGACACAG TAGCCGACAG GGTTGAAATG TCAGTGAATG ACAAAGCAGA AGCAGGTGGT 
TGCAGAAGAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1L ( XM_024976424.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832192.1 Bos taurus tRNA methyltransferase 1 like (TRMT1L), transcript variant X2, mRNA.

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CloneID	OBa223707
Clone ID Related Accession (Same CDS sequence)	XM_024976424.1
Accession Version	XM_024976424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	TRMT1-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCAGGAGA AGGTGGAGGT GGTCCAGGTC 
CCGGTCCCGA CCCCGACCCC GGACTCGGCT CGGGTCCCAG CACCGGCCCC GGATTCGGCT 
CCGGTCTCGG CCTCGACCCC GGCCCCGGCC TCGGCTCCGA CTCCGGCCTC GGCTCCAGTC 
CCAGCCCCTG CCCTGGCCCA GGCTTCGGCC CTGTCCCCGT CCCTAGCCTC TGCCCCTGAT 
GAGGCTGAAA GCAATGGTCT AGTTAGCCCT TTGAAATCAG TCTCATATAG GCAAGATGAA 
GATGGCATAT CTGAAGTAGA ATTGAAAATT GAGGAAGAAA AAGAAGTTTT AAAAGAACCG 
CCTAGCATCT TGGAATCATC TGATTTAATA AGAGAGAGAC ACATCTCAAT TCAAAGGCAG 
CTTGCTGATT TAGAGAAGTT AGCTTTCGTA ACTGAAGGGG ATTGTGACTC TGCCAATTCA 
CTGAACTCAG ATAATCTTGA TGCAGGAAAC AAACAGGCTT GTCCATTGTG CCCCAAGGAG 
AAATTCAGAG CTTGTAATAG TCACAAGCTT CATCGTCACC TTCAGAATTT ACACTGGAAA 
GTCTCCGTCG AATTTGAAGC AACAATCACC AGAAGGACAG ATATGCTAGG ACATGTCAGG 
CGCCATGTGA ACAAAGGAGA GACTAAATCC AGGTATATTG CTGCTTCTGC TGCTAAACCA 
CCTAAAGAAA TTTTGAAAGA GGCAGACACA GATGTACAAG TTTGTCCTAA CTATACTGTA 
CCTCAGAAAA CAGATTCTTA TTTTAATCCC AAAATGAAAC TGAATCGGCA GCTGATATTC 
TGTACACTGG CTGCTCTGGC TAAGGAGCGA AAACCTTTGG AATGTCTAGA TGCCTTTGGA 
GCAACTGGGA TAATGGGATT ACAATGGGCA AAACATCTTG GAAATGCAGT CAAGGTTACA 
ATTAATGATT TGAATGAAAA CTCTGTGACG TTGATTCAGG AGAACTGCCA TTTAAACAAA 
TTGAAAGTGG TTGTGGACAG TAAGGAAAAG GAAGAAAGGG AAGATATTCT GGAAGAAGGA 
GAGGAAAACC TTGGTAATAT TAAGGTGACC AAAATGGATG CCAATGTACT AATGCATTTG 
AGATCTTTTG ATTTCATACA CCTAGATCCT TTTGGAACAT CTGTGAACTA TCTTGATTCA 
GCATTCAGAA ATATAAGAAA CCTTGGCATA GTGTCAGTGA CTTCTACAGA TATCAGTTCT 
TTATATGCCA AGGCACAACA CGTTGCCCGG CGTCACTACG GCTGTAATAT TGTCCGAACT 
GAATATTACA AGGAGCTAGC TGCAAGAATT GTTGTAGCTG CAGTGGCAAG AGCTGCAGCC 
CGATGTAACA AAGGCATAGA AGTACTATTT GCGGTGGCTC TGGAACATTT TGTGTTGGTT 
GTTGTGAGAG TTTTGAGGGG GCCAACTTCT GCAGATGAGA CAGCCAAGAA GATTCAGTAC 
CTAATCCATT GTCAGTGGTG TGAAGAGAGA ATTTTTCAGA AGGATGGTAA TATGGTAGAA 
GAAAACCCAT ATAGACAGCT ACCCTGTAAT TGTCATGGAA GCATGCCTGG AAAGACAGCA 
ATAGAACTTG GACCTCTATG GTCAAGTTCC CTTTTCAATA CTGGATTCCT CAAAAGAATG 
CTGTCTGAAT CTCTTCACCA TGGTTTAGAT GACATTCAGA CCCTAATAAA GACATTAATC 
TTTGAGTCAG AGTGTACTCC TCAGTTTTCA GTTCATGCAC CTTCAAATCT CAACAAGCCA 
GAAGAATGCG GTGTATTTAT TAAAACTACA GACGATACCA CAACAGATAG TCACAGCGCA 
CAAGGAAAGA GAAAAAGTAA TGAAACAACT GCAAATTTAG TCAAGAGGCA AAAGACTGAT 
GTCAATACTG AACATCCTCC CTTTTATTAC AACATCCACA GACACAGCAT TAAAGGAATG 
AATATGCCAA AGTTAAAAAA GTTTTTGTGC TATCTTTCTC AAGCAGGCTT TCGAGTAAGC 
CGAACTCATT TTGACCCAAT GGGAGTTCGT ACAGATGCAC CTCTAATGCA GTTTAAATCC 
ATCCTTTTGA AGTACAGCAC CCCTACTTAC ACCGGAGGAC AGTCAGAGGG CCATGTCCAA 
CCAGCATCTG AAGACACAGT AGCCGACAGG GTTGAAATGT CAGTGAATGA CAAAGCAGAA 
GCAGGTGGTT GCAGAAGATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832192.1
CDS	343..2586
Translation	MENMAEEELLPQEKVEVVQVPVPTPTPDSARVPAPAPDSAPVSASTPAPASAPTPASAPVPAPALAQASALSPSLASAPDEAESNGLVSPLKSVSYRQDEDGISEVELKIEEEKEVLKEPPSILESSDLIRERHISIQRQLADLEKLAFVTEGDCDSANSLNSDNLDAGNKQACPLCPKEKFRACNSHKLHRHLQNLHWKVSVEFEATITRRTDMLGHVRRHVNKGETKSRYIAASAAKPPKEILKEADTDVQVCPNYTVPQKTDSYFNPKMKLNRQLIFCTLAALAKERKPLECLDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEEREDILEEGEENLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEENPYRQLPCNCHGSMPGKTAIELGPLWSSSLFNTGFLKRMLSESLHHGLDDIQTLIKTLIFESECTPQFSVHAPSNLNKPEECGVFIKTTDDTTTDSHSAQGKRKSNETTANLVKRQKTDVNTEHPPFYYNIHRHSIKGMNMPKLKKFLCYLSQAGFRVSRTHFDPMGVRTDAPLMQFKSILLKYSTPTYTGGQSEGHVQPASEDTVADRVEMSVNDKAEAGGCRRW

Target ORF information:

RefSeq Version	XM_024976424.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 1 like (TRMT1L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976424.1

ORF Insert Sequence:

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCAGGAGA AGGTGGAGGT GGTCCAGGTC 
CCGGTCCCGA CCCCGACCCC GGACTCGGCT CGGGTCCCAG CACCGGCCCC GGATTCGGCT 
CCGGTCTCGG CCTCGACCCC GGCCCCGGCC TCGGCTCCGA CTCCGGCCTC GGCTCCAGTC 
CCAGCCCCTG CCCTGGCCCA GGCTTCGGCC CTGTCCCCGT CCCTAGCCTC TGCCCCTGAT 
GAGGCTGAAA GCAATGGTCT AGTTAGCCCT TTGAAATCAG TCTCATATAG GCAAGATGAA 
GATGGCATAT CTGAAGTAGA ATTGAAAATT GAGGAAGAAA AAGAAGTTTT AAAAGAACCG 
CCTAGCATCT TGGAATCATC TGATTTAATA AGAGAGAGAC ACATCTCAAT TCAAAGGCAG 
CTTGCTGATT TAGAGAAGTT AGCTTTCGTA ACTGAAGGGG ATTGTGACTC TGCCAATTCA 
CTGAACTCAG ATAATCTTGA TGCAGGAAAC AAACAGGCTT GTCCATTGTG CCCCAAGGAG 
AAATTCAGAG CTTGTAATAG TCACAAGCTT CATCGTCACC TTCAGAATTT ACACTGGAAA 
GTCTCCGTCG AATTTGAAGC AACAATCACC AGAAGGACAG ATATGCTAGG ACATGTCAGG 
CGCCATGTGA ACAAAGGAGA GACTAAATCC AGGTATATTG CTGCTTCTGC TGCTAAACCA 
CCTAAAGAAA TTTTGAAAGA GGCAGACACA GATGTACAAG TTTGTCCTAA CTATACTGTA 
CCTCAGAAAA CAGATTCTTA TTTTAATCCC AAAATGAAAC TGAATCGGCA GCTGATATTC 
TGTACACTGG CTGCTCTGGC TAAGGAGCGA AAACCTTTGG AATGTCTAGA TGCCTTTGGA 
GCAACTGGGA TAATGGGATT ACAATGGGCA AAACATCTTG GAAATGCAGT CAAGGTTACA 
ATTAATGATT TGAATGAAAA CTCTGTGACG TTGATTCAGG AGAACTGCCA TTTAAACAAA 
TTGAAAGTGG TTGTGGACAG TAAGGAAAAG GAAGAAAGGG AAGATATTCT GGAAGAAGGA 
GAGGAAAACC TTGGTAATAT TAAGGTGACC AAAATGGATG CCAATGTACT AATGCATTTG 
AGATCTTTTG ATTTCATACA CCTAGATCCT TTTGGAACAT CTGTGAACTA TCTTGATTCA 
GCATTCAGAA ATATAAGAAA CCTTGGCATA GTGTCAGTGA CTTCTACAGA TATCAGTTCT 
TTATATGCCA AGGCACAACA CGTTGCCCGG CGTCACTACG GCTGTAATAT TGTCCGAACT 
GAATATTACA AGGAGCTAGC TGCAAGAATT GTTGTAGCTG CAGTGGCAAG AGCTGCAGCC 
CGATGTAACA AAGGCATAGA AGTACTATTT GCGGTGGCTC TGGAACATTT TGTGTTGGTT 
GTTGTGAGAG TTTTGAGGGG GCCAACTTCT GCAGATGAGA CAGCCAAGAA GATTCAGTAC 
CTAATCCATT GTCAGTGGTG TGAAGAGAGA ATTTTTCAGA AGGATGGTAA TATGGTAGAA 
GAAAACCCAT ATAGACAGCT ACCCTGTAAT TGTCATGGAA GCATGCCTGG AAAGACAGCA 
ATAGAACTTG GACCTCTATG GTCAAGTTCC CTTTTCAATA CTGGATTCCT CAAAAGAATG 
CTGTCTGAAT CTCTTCACCA TGGTTTAGAT GACATTCAGA CCCTAATAAA GACATTAATC 
TTTGAGTCAG AGTGTACTCC TCAGTTTTCA GTTCATGCAC CTTCAAATCT CAACAAGCCA 
GAAGAATGCG GTGTATTTAT TAAAACTACA GACGATACCA CAACAGATAG TCACAGCGCA 
CAAGGAAAGA GAAAAAGTAA TGAAACAACT GCAAATTTAG TCAAGAGGCA AAAGACTGAT 
GTCAATACTG AACATCCTCC CTTTTATTAC AACATCCACA GACACAGCAT TAAAGGAATG 
AATATGCCAA AGTTAAAAAA GTTTTTGTGC TATCTTTCTC AAGCAGGCTT TCGAGTAAGC 
CGAACTCATT TTGACCCAAT GGGAGTTCGT ACAGATGCAC CTCTAATGCA GTTTAAATCC 
ATCCTTTTGA AGTACAGCAC CCCTACTTAC ACCGGAGGAC AGTCAGAGGG CCATGTCCAA 
CCAGCATCTG AAGACACAGT AGCCGACAGG GTTGAAATGT CAGTGAATGA CAAAGCAGAA 
GCAGGTGGTT GCAGAAGATG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1L ( XM_005217380.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217437.1 Bos taurus tRNA methyltransferase 1 like (TRMT1L), transcript variant X1, mRNA.

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CloneID	OBa27532
Clone ID Related Accession (Same CDS sequence)	XM_005217380.3
Accession Version	XM_005217380.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	TRMT1-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217380.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCAGGAGA AGGTGGAGGT GGTCCAGGTC 
CCGGTCCCGA CCCCGACCCC GGACTCGGCT CGGGTCCCAG CACCGGCCCC GGATTCGGCT 
CCGGTCTCGG CCTCGACCCC GGCCCCGGCC TCGGCTCCGA CTCCGGCCTC GGCTCCAGTC 
CCAGCCCCTG CCCTGGCCCA GGCTTCGGCC CTGTCCCCGT CCCTAGCCTC TGCCCCTGAT 
GAGGCTGAAA GCAATGGTCT AGTTAGCCCT TTGAAATCAG TCTCATATAG GCAAGATGAA 
GATGGCATAT CTGAAGTAGA ATTGAAAATT GAGGAAGAAA AAGAAGTTTT AAAAGAACCG 
CCTAGCATCT TGGAATCATC TGATTTAATA AGAGAGAGAC ACATCTCAAT TCAAAGGCAG 
CTTGCTGATT TAGAGAAGTT AGCTTTCGTA ACTGAAGGGG ATTGTGACTC TGCCAATTCA 
CTGAACTCAG ATAATCTTGA TGCAGGAAAC AAACAGGCTT GTCCATTGTG CCCCAAGGAG 
AAATTCAGAG CTTGTAATAG TCACAAGCTT CATCGTCACC TTCAGAATTT ACACTGGAAA 
GTCTCCGTCG AATTTGAAGG TTACCGGATG TGCATCTGTC ACTTACCTTG TCGACCGGTG 
AAACCAAACA TTATTGGAGA ACAGATATCC AGTAAAATGG GCGCCCATTA TCATTGTATC 
ATTTGTTCAG CAACAATCAC CAGAAGGACA GATATGCTAG GACATGTCAG GCGCCATGTG 
AACAAAGGAG AGACTAAATC CAGGTATATT GCTGCTTCTG CTGCTAAACC ACCTAAAGAA 
ATTTTGAAAG AGGCAGACAC AGATGTACAA GTTTGTCCTA ACTATACTGT ACCTCAGAAA 
ACAGATTCTT ATTTTAATCC CAAAATGAAA CTGAATCGGC AGCTGATATT CTGTACACTG 
GCTGCTCTGG CTAAGGAGCG AAAACCTTTG GAATGTCTAG ATGCCTTTGG AGCAACTGGG 
ATAATGGGAT TACAATGGGC AAAACATCTT GGAAATGCAG TCAAGGTTAC AATTAATGAT 
TTGAATGAAA ACTCTGTGAC GTTGATTCAG GAGAACTGCC ATTTAAACAA ATTGAAAGTG 
GTTGTGGACA GTAAGGAAAA GGAAGAAAGG GAAGATATTC TGGAAGAAGG AGAGGAAAAC 
CTTGGTAATA TTAAGGTGAC CAAAATGGAT GCCAATGTAC TAATGCATTT GAGATCTTTT 
GATTTCATAC ACCTAGATCC TTTTGGAACA TCTGTGAACT ATCTTGATTC AGCATTCAGA 
AATATAAGAA ACCTTGGCAT AGTGTCAGTG ACTTCTACAG ATATCAGTTC TTTATATGCC 
AAGGCACAAC ACGTTGCCCG GCGTCACTAC GGCTGTAATA TTGTCCGAAC TGAATATTAC 
AAGGAGCTAG CTGCAAGAAT TGTTGTAGCT GCAGTGGCAA GAGCTGCAGC CCGATGTAAC 
AAAGGCATAG AAGTACTATT TGCGGTGGCT CTGGAACATT TTGTGTTGGT TGTTGTGAGA 
GTTTTGAGGG GGCCAACTTC TGCAGATGAG ACAGCCAAGA AGATTCAGTA CCTAATCCAT 
TGTCAGTGGT GTGAAGAGAG AATTTTTCAG AAGGATGGTA ATATGGTAGA AGAAAACCCA 
TATAGACAGC TACCCTGTAA TTGTCATGGA AGCATGCCTG GAAAGACAGC AATAGAACTT 
GGACCTCTAT GGTCAAGTTC CCTTTTCAAT ACTGGATTCC TCAAAAGAAT GCTGTCTGAA 
TCTCTTCACC ATGGTTTAGA TGACATTCAG ACCCTAATAA AGACATTAAT CTTTGAGTCA 
GAGTGTACTC CTCAGTTTTC AGTTCATGCA CCTTCAAATC TCAACAAGCA AGAAGAATGC 
GGTGTATTTA TTAAAACTAC AGACGATACC ACAACAGATA GTCACAGCGC ACAAGGAAAG 
AGAAAAAGTA ATGAAACAAC TGCAAATTTA GTCAAGAGGC AAAAGACTGA TGTCAATACT 
GAACATCCTC CCTTTTATTA CAACATCCAC AGACACAGCA TTAAAGGAAT GAATATGCCA 
AAGTTAAAAA AGTTTTTGTG CTATCTTTCT CAAGCAGGCT TTCGAGTAAG CCGAACTCAT 
TTTGACCCAA TGGGAGTTCG TACAGATGCA CCTCTAATGC AGTTTAAATC CATCCTTTTG 
AAGTACAGCA CCCCTACTTA CACCGGAGGA CAGTCAGAGG GCCATGTCCA ACCAGCATCT 
GAAGACACAG TAGCCGACAG GGTTGAAATG TCAGTGAATG ACAAAGCAGA AGCAGGTGGT 
TGCAGAAGAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217437.1
CDS	343..2697
Translation	MENMAEEELLPQEKVEVVQVPVPTPTPDSARVPAPAPDSAPVSASTPAPASAPTPASAPVPAPALAQASALSPSLASAPDEAESNGLVSPLKSVSYRQDEDGISEVELKIEEEKEVLKEPPSILESSDLIRERHISIQRQLADLEKLAFVTEGDCDSANSLNSDNLDAGNKQACPLCPKEKFRACNSHKLHRHLQNLHWKVSVEFEGYRMCICHLPCRPVKPNIIGEQISSKMGAHYHCIICSATITRRTDMLGHVRRHVNKGETKSRYIAASAAKPPKEILKEADTDVQVCPNYTVPQKTDSYFNPKMKLNRQLIFCTLAALAKERKPLECLDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEEREDILEEGEENLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEENPYRQLPCNCHGSMPGKTAIELGPLWSSSLFNTGFLKRMLSESLHHGLDDIQTLIKTLIFESECTPQFSVHAPSNLNKQEECGVFIKTTDDTTTDSHSAQGKRKSNETTANLVKRQKTDVNTEHPPFYYNIHRHSIKGMNMPKLKKFLCYLSQAGFRVSRTHFDPMGVRTDAPLMQFKSILLKYSTPTYTGGQSEGHVQPASEDTVADRVEMSVNDKAEAGGCRRW

Target ORF information:

RefSeq Version	XM_005217380.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 1 like (TRMT1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217380.3

ORF Insert Sequence:

ATGGAGAATA TGGCGGAGGA GGAGCTGCTG CCCCAGGAGA AGGTGGAGGT GGTCCAGGTC 
CCGGTCCCGA CCCCGACCCC GGACTCGGCT CGGGTCCCAG CACCGGCCCC GGATTCGGCT 
CCGGTCTCGG CCTCGACCCC GGCCCCGGCC TCGGCTCCGA CTCCGGCCTC GGCTCCAGTC 
CCAGCCCCTG CCCTGGCCCA GGCTTCGGCC CTGTCCCCGT CCCTAGCCTC TGCCCCTGAT 
GAGGCTGAAA GCAATGGTCT AGTTAGCCCT TTGAAATCAG TCTCATATAG GCAAGATGAA 
GATGGCATAT CTGAAGTAGA ATTGAAAATT GAGGAAGAAA AAGAAGTTTT AAAAGAACCG 
CCTAGCATCT TGGAATCATC TGATTTAATA AGAGAGAGAC ACATCTCAAT TCAAAGGCAG 
CTTGCTGATT TAGAGAAGTT AGCTTTCGTA ACTGAAGGGG ATTGTGACTC TGCCAATTCA 
CTGAACTCAG ATAATCTTGA TGCAGGAAAC AAACAGGCTT GTCCATTGTG CCCCAAGGAG 
AAATTCAGAG CTTGTAATAG TCACAAGCTT CATCGTCACC TTCAGAATTT ACACTGGAAA 
GTCTCCGTCG AATTTGAAGG TTACCGGATG TGCATCTGTC ACTTACCTTG TCGACCGGTG 
AAACCAAACA TTATTGGAGA ACAGATATCC AGTAAAATGG GCGCCCATTA TCATTGTATC 
ATTTGTTCAG CAACAATCAC CAGAAGGACA GATATGCTAG GACATGTCAG GCGCCATGTG 
AACAAAGGAG AGACTAAATC CAGGTATATT GCTGCTTCTG CTGCTAAACC ACCTAAAGAA 
ATTTTGAAAG AGGCAGACAC AGATGTACAA GTTTGTCCTA ACTATACTGT ACCTCAGAAA 
ACAGATTCTT ATTTTAATCC CAAAATGAAA CTGAATCGGC AGCTGATATT CTGTACACTG 
GCTGCTCTGG CTAAGGAGCG AAAACCTTTG GAATGTCTAG ATGCCTTTGG AGCAACTGGG 
ATAATGGGAT TACAATGGGC AAAACATCTT GGAAATGCAG TCAAGGTTAC AATTAATGAT 
TTGAATGAAA ACTCTGTGAC GTTGATTCAG GAGAACTGCC ATTTAAACAA ATTGAAAGTG 
GTTGTGGACA GTAAGGAAAA GGAAGAAAGG GAAGATATTC TGGAAGAAGG AGAGGAAAAC 
CTTGGTAATA TTAAGGTGAC CAAAATGGAT GCCAATGTAC TAATGCATTT GAGATCTTTT 
GATTTCATAC ACCTAGATCC TTTTGGAACA TCTGTGAACT ATCTTGATTC AGCATTCAGA 
AATATAAGAA ACCTTGGCAT AGTGTCAGTG ACTTCTACAG ATATCAGTTC TTTATATGCC 
AAGGCACAAC ACGTTGCCCG GCGTCACTAC GGCTGTAATA TTGTCCGAAC TGAATATTAC 
AAGGAGCTAG CTGCAAGAAT TGTTGTAGCT GCAGTGGCAA GAGCTGCAGC CCGATGTAAC 
AAAGGCATAG AAGTACTATT TGCGGTGGCT CTGGAACATT TTGTGTTGGT TGTTGTGAGA 
GTTTTGAGGG GGCCAACTTC TGCAGATGAG ACAGCCAAGA AGATTCAGTA CCTAATCCAT 
TGTCAGTGGT GTGAAGAGAG AATTTTTCAG AAGGATGGTA ATATGGTAGA AGAAAACCCA 
TATAGACAGC TACCCTGTAA TTGTCATGGA AGCATGCCTG GAAAGACAGC AATAGAACTT 
GGACCTCTAT GGTCAAGTTC CCTTTTCAAT ACTGGATTCC TCAAAAGAAT GCTGTCTGAA 
TCTCTTCACC ATGGTTTAGA TGACATTCAG ACCCTAATAA AGACATTAAT CTTTGAGTCA 
GAGTGTACTC CTCAGTTTTC AGTTCATGCA CCTTCAAATC TCAACAAGCA AGAAGAATGC 
GGTGTATTTA TTAAAACTAC AGACGATACC ACAACAGATA GTCACAGCGC ACAAGGAAAG 
AGAAAAAGTA ATGAAACAAC TGCAAATTTA GTCAAGAGGC AAAAGACTGA TGTCAATACT 
GAACATCCTC CCTTTTATTA CAACATCCAC AGACACAGCA TTAAAGGAAT GAATATGCCA 
AAGTTAAAAA AGTTTTTGTG CTATCTTTCT CAAGCAGGCT TTCGAGTAAG CCGAACTCAT 
TTTGACCCAA TGGGAGTTCG TACAGATGCA CCTCTAATGC AGTTTAAATC CATCCTTTTG 
AAGTACAGCA CCCCTACTTA CACCGGAGGA CAGTCAGAGG GCCATGTCCA ACCAGCATCT 
GAAGACACAG TAGCCGACAG GGTTGAAATG TCAGTGAATG ACAAAGCAGA AGCAGGTGGT 
TGCAGAAGAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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TRMT1L cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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