MARCHF6 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MARCHF6
Entrez Gene ID	540371
Full Name	membrane associated ring-CH-type finger 6
General protein information	Preferred Names membrane associated ring-CH-type finger 6 Names E3 ubiquitin-protein ligase MARCH6 membrane-associated ring finger (C3HC4) 6, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024981357.1	XP_024837125.1	E3 ubiquitin-protein ligase MARCH6 isoform X1
XM_024981358.1	XP_024837126.1	E3 ubiquitin-protein ligase MARCH6 isoform X1
NM_001205812.2	NP_001192741.1	E3 ubiquitin-protein ligase MARCH6
NM_001205812.1	NP_001192741.1	E3 ubiquitin-protein ligase MARCH6
NM_001205812.3	NP_001192741.1	E3 ubiquitin-protein ligase MARCH6

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MARCH6	XP_005639724.1
Gallus gallus (chicken)	MARCH6	XP_419012.3
Arabidopsis thaliana (thale cress)	AT4G34100	NP_195136.3
Pan troglodytes (chimpanzee)	MARCH6	XP_003310676.1
Mus musculus (house mouse)	March6	NP_766194.2
Drosophila melanogaster (fruit fly)	CG1317	NP_647715.2
Xenopus tropicalis (tropical clawed frog)	march6	XP_002938364.2
Homo sapiens (human)	MARCH6	NP_005876.2
Bos taurus (cattle)	MARCH6	NP_001192741.1
Oryza sativa (rice)	Os06g0639000	NP_001058157.1
Rattus norvegicus (Norway rat)	March6	XP_215517.5
Caenorhabditis elegans (roundworm)	marc-6	NP_492823.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007204	XP_308581.3
Danio rerio (zebrafish)	march6	XP_005163506.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MARCHF6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MARCHF6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01698	NM_001205812.3 Latest version!	Bos taurus membrane associated ring-CH-type finger 6 (MARCHF6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa228061	XM_024981357.1 Latest version!	Bos taurus membrane associated ring-CH-type finger 6 (MARCH6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa228061	XM_024981358.1 Latest version!	Bos taurus membrane associated ring-CH-type finger 6 (MARCH6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OBa01698	NM_001205812.1	Bos taurus membrane-associated ring finger (C3HC4) 6 (MARCH6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa01698	NM_001205812.2	Bos taurus membrane associated ring-CH-type finger 6 (MARCH6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MARCHF6 ( NM_001205812.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192741.1 Bos taurus membrane associated ring-CH-type finger 6 (MARCH6), mRNA.

Price & Availability↑

CloneID	OBa01698
Clone ID Related Accession (Same CDS sequence)	NM_001205812.2 , NM_001205812.1 , NM_001205812.3
Accession Version	NM_001205812.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1508601600000
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase MARCH6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02051219.1, EV626671.1, EV632242.1 and EV706277.1. On Aug 18, 2017 this sequence version replaced NM_001205812.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCG CGGAGGAAGA CATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTATC ATCCTTGTGT GTGTACTGGC AGTATTAAGT TTATCCATCA AGAATGCTTA 
GTTCAGTGGC TGAAACACAG TCGAAAAGAA TACTGTGAAT TATGCAAGCA CAGGTTTGCT 
TTCACACCAA TTTATTCCCC AGATATGCCT TCACGGCTTC CAATCCAGGA CATATTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCAATA CGATATTGGT TTCATTATAC ACTTGTGGCC 
TTTGCATGGT TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCTGTGA GCTCCCTCCT GACGCTGCCG CTGGACATGC TGTCCACGGA CAACTTGTTG 
GCGGATTGTT TGCAGGGTTG TTTTGTGGTG ACGTGCACAC TGTGCGCGTT CATCAGCCTG 
GTGTGGCTGC GAGAGCAGAT CGTCCACGGG GGAGCCCCAA TCTGGCTGGA GCATGCCGCT 
CCGCCCTTCA ATGCTGCTGG GCATCACCAA AATGAGGCTC CAGCTGGAGG AAATGGTGCT 
GAAAATGTTG CCCCCGAGCA GCCTGCTAAC CCCCCAGCAG AGAACGCGGT GGTGGGGGAA 
AACCCTGATG CCCAGGATGA GCAGGCGGAG GAGGAGGAGG AGGAGAACGA GGAGGAAGAC 
GACGCGGGCG GGGAGGATGC CGCTGACGCC AACAACGGGG TGCAGGATGA CATGAACTGG 
AATGCTTTAG AATGGGACCG AGCTGCAGAA GAGCTTACGT GGGAACGAAT GCTAGGACTC 
GATGGATCAC TAGTTTTTCT AGAACATGTC TTTTGGGTGG TGTCTTTAAA CACATTGTTC 
ATTCTCGTTT TTGCATTTTG CCCTTACCAC ATCGGTCACT TTTCCCTTGT TGGTTTGGGA 
TTTGAAGAAC ACGTCCAAGC ATCTCATTTT GAAGGCCTAA TCACAACCAT AGTTGGGTAT 
ATACTTTTAG CAATAACATT GATAATTTGT CATGGGTTGG CAACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG AGTCTGCTAT ATTGTTGTGA AGGTCTCTTT GTTAGTGGTG 
GTAGAAATTG GAGTTTTCCC TCTCATTTGT GGCTGGTGGC TGGATATCTG TTCCTTGGAA 
ATGTTTGACG CTACTCTGAA AGATCGGGAG CTGAGCTTTC AGTCGGCCCC AGGCACTACC 
ATGTTCCTGC ACTGGCTGGT GGGAATGGTG TATGTGTTCT ACTTTGCCTC CTTCATTCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTT TTATGGTTTC TAAGGAATTT GAATGATCCA 
GATTTTAATC CAGTACAAGA AATGATTCAC TTGCCTATTT ATAGGCATCT TCGAAGATTT 
ATTTTGTCAG TGATTGTCTT CGGCTCCATC GTACTCCTAA TGCTGTGGCT GCCTATACGG 
ATAATCAAGA GTCTGCTGCC TCATTTTCTT CCATACAACG TCATGCTCTA TAGTGATGCT 
CCAGTGAGTG AACTGTCCCT TGAGCTGCTT CTGCTTCAGG TTGTCTTACC AGCGTTACTC 
GAACAGGGAC ACACGCGGCA GTGGCTGAAG GGACTGGTGC GAGCCTGGAC TGTCACTGCT 
GGATACTTGC TGGATCTTCA TTCCTACTTG TTGGGAGACC AGGAAGAAAA TGAGAACAGT 
GCAAACCAAC AGGTTAACAA TAACCAGCAT GCTCGAAATA ACAACGCTAT TCCCGTGGTA 
GGGGAAGGCC TGCACGCAGC CCACCAAGCC ATACTCCAGC AGGGCGGGCC TGTTGGCTTT 
CAGCCTTACC GCCGGCCTTT AAATTTTCCA CTCAGGATAT TTTTGTTGAT TGTCTTCATG 
TGTATAACCT TACTGATTGC CAGCCTCATC TGCCTTACTC TACCAGTATT TGCTGGCCGT 
TGGTTAATGT CATTTTGGAC CGGGACTGCC AAAATCCATG AGCTCTACAC AGCTGCCTGT 
GGTCTCTATG TTTGCTGGCT CACCATCCGG GCGGTGACGG TACTGGTGGC GTGGATGCCA 
CAGGGACGCA GAGTGATCTT CCAGAAGGTT AAGGAGTGGT CCCTCATGAT AATGAAGACT 
GTGATCGTGG CAGTGCTGCT GGCTGGGGTG GTCCCGCTTC TTCTGGGGCT TCTGTTTGAG 
CTGGTTATTG TTGCTCCCCT GAGGGTTCCT TTGGATCAGA CTCCTCTTTT TTACCCATGG 
CAGGACTGGG CACTTGGAGT CCTGCATGCC AAAATCATTG CAGCTATAAC ATTGATGGGT 
CCTCAGTGGT GGCTGAAAAC TGTAATTGAG CAGGTTTACG CAAATGGCAT CCGGAACATT 
GACCTTCAGT ACATCGTTCG GAAACTGGCA GCGCCAGTGA TCTCTGTGCT CTTGCTTTCC 
CTGTGTGTAC CTTACGTCAT CGCGTCCGGC ATCGTTCCTT TACTCGGTGT TACTGCGGAA 
ATGCAAAATT TGGTCCATCG GCGGATTTAT CCATTTTTGC TGATGGTTGT GGTGTTGATG 
GGAATCTTGT CCTTCCAGGT CCGCCAGTTT AAGCGCCTTT ATGAACATAT TAAAAATGAC 
AAGTACCTTG TGGGCCAGCG CCTCGTGAAC TATGAACGGA AATCCGGCAA ACAAGGCACA 
TCACCACCAC CTCCACAGTC GTCCCAAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192741.1
CDS	12..2744
Translation	MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTDNLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPAGGNGAENVAPEQPANPPAENAVVGENPDAQDEQAEEEEEENEEEDDAGGEDAADANNGVQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHGLATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSLLPHFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQHARNNNAIPVVGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVLVAWMPQGRRVIFQKVKEWSLMIMKTVIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLQYIVRKLAAPVISVLLLSLCVPYVIASGIVPLLGVTAEMQNLVHRRIYPFLLMVVVLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGTSPPPPQSSQE

Target ORF information:

RefSeq Version	NM_001205812.2
Organism	Bos taurus(cattle)
Definition	Bos taurus membrane associated ring-CH-type finger 6 (MARCH6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205812.2

ORF Insert Sequence:

ATGGACACCG CGGAGGAAGA CATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTATC ATCCTTGTGT GTGTACTGGC AGTATTAAGT TTATCCATCA AGAATGCTTA 
GTTCAGTGGC TGAAACACAG TCGAAAAGAA TACTGTGAAT TATGCAAGCA CAGGTTTGCT 
TTCACACCAA TTTATTCCCC AGATATGCCT TCACGGCTTC CAATCCAGGA CATATTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCAATA CGATATTGGT TTCATTATAC ACTTGTGGCC 
TTTGCATGGT TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCTGTGA GCTCCCTCCT GACGCTGCCG CTGGACATGC TGTCCACGGA CAACTTGTTG 
GCGGATTGTT TGCAGGGTTG TTTTGTGGTG ACGTGCACAC TGTGCGCGTT CATCAGCCTG 
GTGTGGCTGC GAGAGCAGAT CGTCCACGGG GGAGCCCCAA TCTGGCTGGA GCATGCCGCT 
CCGCCCTTCA ATGCTGCTGG GCATCACCAA AATGAGGCTC CAGCTGGAGG AAATGGTGCT 
GAAAATGTTG CCCCCGAGCA GCCTGCTAAC CCCCCAGCAG AGAACGCGGT GGTGGGGGAA 
AACCCTGATG CCCAGGATGA GCAGGCGGAG GAGGAGGAGG AGGAGAACGA GGAGGAAGAC 
GACGCGGGCG GGGAGGATGC CGCTGACGCC AACAACGGGG TGCAGGATGA CATGAACTGG 
AATGCTTTAG AATGGGACCG AGCTGCAGAA GAGCTTACGT GGGAACGAAT GCTAGGACTC 
GATGGATCAC TAGTTTTTCT AGAACATGTC TTTTGGGTGG TGTCTTTAAA CACATTGTTC 
ATTCTCGTTT TTGCATTTTG CCCTTACCAC ATCGGTCACT TTTCCCTTGT TGGTTTGGGA 
TTTGAAGAAC ACGTCCAAGC ATCTCATTTT GAAGGCCTAA TCACAACCAT AGTTGGGTAT 
ATACTTTTAG CAATAACATT GATAATTTGT CATGGGTTGG CAACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG AGTCTGCTAT ATTGTTGTGA AGGTCTCTTT GTTAGTGGTG 
GTAGAAATTG GAGTTTTCCC TCTCATTTGT GGCTGGTGGC TGGATATCTG TTCCTTGGAA 
ATGTTTGACG CTACTCTGAA AGATCGGGAG CTGAGCTTTC AGTCGGCCCC AGGCACTACC 
ATGTTCCTGC ACTGGCTGGT GGGAATGGTG TATGTGTTCT ACTTTGCCTC CTTCATTCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTT TTATGGTTTC TAAGGAATTT GAATGATCCA 
GATTTTAATC CAGTACAAGA AATGATTCAC TTGCCTATTT ATAGGCATCT TCGAAGATTT 
ATTTTGTCAG TGATTGTCTT CGGCTCCATC GTACTCCTAA TGCTGTGGCT GCCTATACGG 
ATAATCAAGA GTCTGCTGCC TCATTTTCTT CCATACAACG TCATGCTCTA TAGTGATGCT 
CCAGTGAGTG AACTGTCCCT TGAGCTGCTT CTGCTTCAGG TTGTCTTACC AGCGTTACTC 
GAACAGGGAC ACACGCGGCA GTGGCTGAAG GGACTGGTGC GAGCCTGGAC TGTCACTGCT 
GGATACTTGC TGGATCTTCA TTCCTACTTG TTGGGAGACC AGGAAGAAAA TGAGAACAGT 
GCAAACCAAC AGGTTAACAA TAACCAGCAT GCTCGAAATA ACAACGCTAT TCCCGTGGTA 
GGGGAAGGCC TGCACGCAGC CCACCAAGCC ATACTCCAGC AGGGCGGGCC TGTTGGCTTT 
CAGCCTTACC GCCGGCCTTT AAATTTTCCA CTCAGGATAT TTTTGTTGAT TGTCTTCATG 
TGTATAACCT TACTGATTGC CAGCCTCATC TGCCTTACTC TACCAGTATT TGCTGGCCGT 
TGGTTAATGT CATTTTGGAC CGGGACTGCC AAAATCCATG AGCTCTACAC AGCTGCCTGT 
GGTCTCTATG TTTGCTGGCT CACCATCCGG GCGGTGACGG TACTGGTGGC GTGGATGCCA 
CAGGGACGCA GAGTGATCTT CCAGAAGGTT AAGGAGTGGT CCCTCATGAT AATGAAGACT 
GTGATCGTGG CAGTGCTGCT GGCTGGGGTG GTCCCGCTTC TTCTGGGGCT TCTGTTTGAG 
CTGGTTATTG TTGCTCCCCT GAGGGTTCCT TTGGATCAGA CTCCTCTTTT TTACCCATGG 
CAGGACTGGG CACTTGGAGT CCTGCATGCC AAAATCATTG CAGCTATAAC ATTGATGGGT 
CCTCAGTGGT GGCTGAAAAC TGTAATTGAG CAGGTTTACG CAAATGGCAT CCGGAACATT 
GACCTTCAGT ACATCGTTCG GAAACTGGCA GCGCCAGTGA TCTCTGTGCT CTTGCTTTCC 
CTGTGTGTAC CTTACGTCAT CGCGTCCGGC ATCGTTCCTT TACTCGGTGT TACTGCGGAA 
ATGCAAAATT TGGTCCATCG GCGGATTTAT CCATTTTTGC TGATGGTTGT GGTGTTGATG 
GGAATCTTGT CCTTCCAGGT CCGCCAGTTT AAGCGCCTTT ATGAACATAT TAAAAATGAC 
AAGTACCTTG TGGGCCAGCG CCTCGTGAAC TATGAACGGA AATCCGGCAA ACAAGGCACA 
TCACCACCAC CTCCACAGTC GTCCCAAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARCHF6 ( NM_001205812.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192741.1 Bos taurus membrane-associated ring finger (C3HC4) 6 (MARCH6), mRNA.

Price & Availability↑

CloneID	OBa01698
Clone ID Related Accession (Same CDS sequence)	NM_001205812.2 , NM_001205812.1 , NM_001205812.3
Accession Version	NM_001205812.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1319385600000
Organism	Bos taurus(Cattle)
Product	E3 ubiquitin-protein ligase MARCH6
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03048513.1. On Apr 20, 2011 this sequence version replaced XM_593602.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGACACCG CGGAGGAAGA CATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTATC ATCCTTGTGT GTGTACTGGC AGTATTAAGT TTATCCATCA AGAATGCTTA 
GTTCAGTGGC TGAAACACAG TCGAAAAGAA TACTGTGAAT TATGCAAGCA CAGGTTTGCT 
TTCACACCAA TTTATTCCCC AGATATGCCT TCACGGCTTC CAATCCAGGA CATATTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCAATA CGATATTGGT TTCATTATAC ACTTGTGGCC 
TTTGCATGGT TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCTGTGA GCTCCCTCCT GACGCTGCCG CTGGACATGC TGTCCACGGA CAACTTGTTG 
GCGGATTGTT TGCAGGGTTG TTTTGTGGTG ACGTGCACAC TGTGCGCGTT CATCAGCCTG 
GTGTGGCTGC GAGAGCAGAT CGTCCACGGG GGAGCCCCAA TCTGGCTGGA GCATGCCGCT 
CCGCCCTTCA ATGCTGCTGG GCATCACCAA AATGAGGCTC CAGCTGGAGG AAATGGTGCT 
GAAAATGTTG CCCCCGAGCA GCCTGCTAAC CCCCCAGCAG AGAACGCGGT GGTGGGGGAA 
AACCCTGATG CCCAGGATGA GCAGGCGGAG GAGGAGGAGG AGGAGAACGA GGAGGAAGAC 
GACGCGGGCG GGGAGGATGC CGCTGACGCC AACAACGGGG TGCAGGATGA CATGAACTGG 
AATGCTTTAG AATGGGACCG AGCTGCAGAA GAGCTTACGT GGGAACGAAT GCTAGGACTC 
GATGGATCAC TAGTTTTTCT AGAACATGTC TTTTGGGTGG TGTCTTTAAA CACATTGTTC 
ATTCTCGTTT TTGCATTTTG CCCTTACCAC ATCGGTCACT TTTCCCTTGT TGGTTTGGGA 
TTTGAAGAAC ACGTCCAAGC ATCTCATTTT GAAGGCCTAA TCACAACCAT AGTTGGGTAT 
ATACTTTTAG CAATAACATT GATAATTTGT CATGGGTTGG CAACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG AGTCTGCTAT ATTGTTGTGA AGGTCTCTTT GTTAGTGGTG 
GTAGAAATTG GAGTTTTCCC TCTCATTTGT GGCTGGTGGC TGGATATCTG TTCCTTGGAA 
ATGTTTGACG CTACTCTGAA AGATCGGGAG CTGAGCTTTC AGTCGGCCCC AGGCACTACC 
ATGTTCCTGC ACTGGCTGGT GGGAATGGTG TATGTGTTCT ACTTTGCCTC CTTCATTCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTT TTATGGTTTC TAAGGAATTT GAATGATCCA 
GATTTTAATC CAGTACAAGA AATGATTCAC TTGCCTATTT ATAGGCATCT TCGAAGATTT 
ATTTTGTCAG TGATTGTCTT CGGCTCCATC GTACTCCTAA TGCTGTGGCT GCCTATACGG 
ATAATCAAGA GTCTGCTGCC TCATTTTCTT CCATACAACG TCATGCTCTA TAGTGATGCT 
CCAGTGAGTG AACTGTCCCT TGAGCTGCTT CTGCTTCAGG TTGTCTTACC AGCGTTACTC 
GAACAGGGAC ACACGCGGCA GTGGCTGAAG GGACTGGTGC GAGCCTGGAC TGTCACTGCT 
GGATACTTGC TGGATCTTCA TTCCTACTTG TTGGGAGACC AGGAAGAAAA TGAGAACAGT 
GCAAACCAAC AGGTTAACAA TAACCAGCAT GCTCGAAATA ACAACGCTAT TCCCGTGGTA 
GGGGAAGGCC TGCACGCAGC CCACCAAGCC ATACTCCAGC AGGGCGGGCC TGTTGGCTTT 
CAGCCTTACC GCCGGCCTTT AAATTTTCCA CTCAGGATAT TTTTGTTGAT TGTCTTCATG 
TGTATAACCT TACTGATTGC CAGCCTCATC TGCCTTACTC TACCAGTATT TGCTGGCCGT 
TGGTTAATGT CATTTTGGAC CGGGACTGCC AAAATCCATG AGCTCTACAC AGCTGCCTGT 
GGTCTCTATG TTTGCTGGCT CACCATCCGG GCGGTGACGG TACTGGTGGC GTGGATGCCA 
CAGGGACGCA GAGTGATCTT CCAGAAGGTT AAGGAGTGGT CCCTCATGAT AATGAAGACT 
GTGATCGTGG CAGTGCTGCT GGCTGGGGTG GTCCCGCTTC TTCTGGGGCT TCTGTTTGAG 
CTGGTTATTG TTGCTCCCCT GAGGGTTCCT TTGGATCAGA CTCCTCTTTT TTACCCATGG 
CAGGACTGGG CACTTGGAGT CCTGCATGCC AAAATCATTG CAGCTATAAC ATTGATGGGT 
CCTCAGTGGT GGCTGAAAAC TGTAATTGAG CAGGTTTACG CAAATGGCAT CCGGAACATT 
GACCTTCAGT ACATCGTTCG GAAACTGGCA GCGCCAGTGA TCTCTGTGCT CTTGCTTTCC 
CTGTGTGTAC CTTACGTCAT CGCGTCCGGC ATCGTTCCTT TACTCGGTGT TACTGCGGAA 
ATGCAAAATT TGGTCCATCG GCGGATTTAT CCATTTTTGC TGATGGTTGT GGTGTTGATG 
GGAATCTTGT CCTTCCAGGT CCGCCAGTTT AAGCGCCTTT ATGAACATAT TAAAAATGAC 
AAGTACCTTG TGGGCCAGCG CCTCGTGAAC TATGAACGGA AATCCGGCAA ACAAGGCACA 
TCACCACCAC CTCCACAGTC GTCCCAAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192741.1
CDS	6..2738
Translation	MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTDNLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPAGGNGAENVAPEQPANPPAENAVVGENPDAQDEQAEEEEEENEEEDDAGGEDAADANNGVQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHGLATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSLLPHFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQHARNNNAIPVVGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVLVAWMPQGRRVIFQKVKEWSLMIMKTVIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLQYIVRKLAAPVISVLLLSLCVPYVIASGIVPLLGVTAEMQNLVHRRIYPFLLMVVVLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGTSPPPPQSSQE

Target ORF information:

RefSeq Version	NM_001205812.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus membrane-associated ring finger (C3HC4) 6 (MARCH6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205812.1

ORF Insert Sequence:

ATGGACACCG CGGAGGAAGA CATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTATC ATCCTTGTGT GTGTACTGGC AGTATTAAGT TTATCCATCA AGAATGCTTA 
GTTCAGTGGC TGAAACACAG TCGAAAAGAA TACTGTGAAT TATGCAAGCA CAGGTTTGCT 
TTCACACCAA TTTATTCCCC AGATATGCCT TCACGGCTTC CAATCCAGGA CATATTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCAATA CGATATTGGT TTCATTATAC ACTTGTGGCC 
TTTGCATGGT TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCTGTGA GCTCCCTCCT GACGCTGCCG CTGGACATGC TGTCCACGGA CAACTTGTTG 
GCGGATTGTT TGCAGGGTTG TTTTGTGGTG ACGTGCACAC TGTGCGCGTT CATCAGCCTG 
GTGTGGCTGC GAGAGCAGAT CGTCCACGGG GGAGCCCCAA TCTGGCTGGA GCATGCCGCT 
CCGCCCTTCA ATGCTGCTGG GCATCACCAA AATGAGGCTC CAGCTGGAGG AAATGGTGCT 
GAAAATGTTG CCCCCGAGCA GCCTGCTAAC CCCCCAGCAG AGAACGCGGT GGTGGGGGAA 
AACCCTGATG CCCAGGATGA GCAGGCGGAG GAGGAGGAGG AGGAGAACGA GGAGGAAGAC 
GACGCGGGCG GGGAGGATGC CGCTGACGCC AACAACGGGG TGCAGGATGA CATGAACTGG 
AATGCTTTAG AATGGGACCG AGCTGCAGAA GAGCTTACGT GGGAACGAAT GCTAGGACTC 
GATGGATCAC TAGTTTTTCT AGAACATGTC TTTTGGGTGG TGTCTTTAAA CACATTGTTC 
ATTCTCGTTT TTGCATTTTG CCCTTACCAC ATCGGTCACT TTTCCCTTGT TGGTTTGGGA 
TTTGAAGAAC ACGTCCAAGC ATCTCATTTT GAAGGCCTAA TCACAACCAT AGTTGGGTAT 
ATACTTTTAG CAATAACATT GATAATTTGT CATGGGTTGG CAACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG AGTCTGCTAT ATTGTTGTGA AGGTCTCTTT GTTAGTGGTG 
GTAGAAATTG GAGTTTTCCC TCTCATTTGT GGCTGGTGGC TGGATATCTG TTCCTTGGAA 
ATGTTTGACG CTACTCTGAA AGATCGGGAG CTGAGCTTTC AGTCGGCCCC AGGCACTACC 
ATGTTCCTGC ACTGGCTGGT GGGAATGGTG TATGTGTTCT ACTTTGCCTC CTTCATTCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTT TTATGGTTTC TAAGGAATTT GAATGATCCA 
GATTTTAATC CAGTACAAGA AATGATTCAC TTGCCTATTT ATAGGCATCT TCGAAGATTT 
ATTTTGTCAG TGATTGTCTT CGGCTCCATC GTACTCCTAA TGCTGTGGCT GCCTATACGG 
ATAATCAAGA GTCTGCTGCC TCATTTTCTT CCATACAACG TCATGCTCTA TAGTGATGCT 
CCAGTGAGTG AACTGTCCCT TGAGCTGCTT CTGCTTCAGG TTGTCTTACC AGCGTTACTC 
GAACAGGGAC ACACGCGGCA GTGGCTGAAG GGACTGGTGC GAGCCTGGAC TGTCACTGCT 
GGATACTTGC TGGATCTTCA TTCCTACTTG TTGGGAGACC AGGAAGAAAA TGAGAACAGT 
GCAAACCAAC AGGTTAACAA TAACCAGCAT GCTCGAAATA ACAACGCTAT TCCCGTGGTA 
GGGGAAGGCC TGCACGCAGC CCACCAAGCC ATACTCCAGC AGGGCGGGCC TGTTGGCTTT 
CAGCCTTACC GCCGGCCTTT AAATTTTCCA CTCAGGATAT TTTTGTTGAT TGTCTTCATG 
TGTATAACCT TACTGATTGC CAGCCTCATC TGCCTTACTC TACCAGTATT TGCTGGCCGT 
TGGTTAATGT CATTTTGGAC CGGGACTGCC AAAATCCATG AGCTCTACAC AGCTGCCTGT 
GGTCTCTATG TTTGCTGGCT CACCATCCGG GCGGTGACGG TACTGGTGGC GTGGATGCCA 
CAGGGACGCA GAGTGATCTT CCAGAAGGTT AAGGAGTGGT CCCTCATGAT AATGAAGACT 
GTGATCGTGG CAGTGCTGCT GGCTGGGGTG GTCCCGCTTC TTCTGGGGCT TCTGTTTGAG 
CTGGTTATTG TTGCTCCCCT GAGGGTTCCT TTGGATCAGA CTCCTCTTTT TTACCCATGG 
CAGGACTGGG CACTTGGAGT CCTGCATGCC AAAATCATTG CAGCTATAAC ATTGATGGGT 
CCTCAGTGGT GGCTGAAAAC TGTAATTGAG CAGGTTTACG CAAATGGCAT CCGGAACATT 
GACCTTCAGT ACATCGTTCG GAAACTGGCA GCGCCAGTGA TCTCTGTGCT CTTGCTTTCC 
CTGTGTGTAC CTTACGTCAT CGCGTCCGGC ATCGTTCCTT TACTCGGTGT TACTGCGGAA 
ATGCAAAATT TGGTCCATCG GCGGATTTAT CCATTTTTGC TGATGGTTGT GGTGTTGATG 
GGAATCTTGT CCTTCCAGGT CCGCCAGTTT AAGCGCCTTT ATGAACATAT TAAAAATGAC 
AAGTACCTTG TGGGCCAGCG CCTCGTGAAC TATGAACGGA AATCCGGCAA ACAAGGCACA 
TCACCACCAC CTCCACAGTC GTCCCAAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARCHF6 ( NM_001205812.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192741.1 Bos taurus membrane associated ring-CH-type finger 6 (MARCHF6), mRNA.

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CloneID	OBa01698
Clone ID Related Accession (Same CDS sequence)	NM_001205812.2 , NM_001205812.1 , NM_001205812.3
Accession Version	NM_001205812.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564416000000
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase MARCH6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000020.1, EV626671.1, EV632242.1 and EV706277.1. On Jun 16, 2018 this sequence version replaced NM_001205812.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571264.21199.1, SRR5571258.1649.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCG CGGAGGAAGA CATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTATC ATCCTTGTGT GTGTACTGGC AGTATTAAGT TTATCCATCA AGAATGCTTA 
GTTCAGTGGC TGAAACACAG TCGAAAAGAA TACTGTGAAT TATGCAAGCA CAGGTTTGCT 
TTCACACCAA TTTATTCCCC AGATATGCCT TCACGGCTTC CAATCCAGGA CATATTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCAATA CGATATTGGT TTCATTATAC ACTTGTGGCC 
TTTGCATGGT TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCTGTGA GCTCCCTCCT GACGCTGCCG CTGGACATGC TGTCCACGGA CAACTTGTTG 
GCGGATTGTT TGCAGGGTTG TTTTGTGGTG ACGTGCACAC TGTGCGCGTT CATCAGCCTG 
GTGTGGCTGC GAGAGCAGAT CGTCCACGGG GGAGCCCCAA TCTGGCTGGA GCATGCCGCT 
CCGCCCTTCA ATGCTGCTGG GCATCACCAA AATGAGGCTC CAGCTGGAGG AAATGGTGCT 
GAAAATGTTG CCCCCGAGCA GCCTGCTAAC CCCCCAGCAG AGAACGCGGT GGTGGGGGAA 
AACCCTGATG CCCAGGATGA GCAGGCGGAG GAGGAGGAGG AGGAGAACGA GGAGGAAGAC 
GACGCGGGCG GGGAGGATGC CGCTGACGCC AACAACGGGG TGCAGGATGA CATGAACTGG 
AATGCTTTAG AATGGGACCG AGCTGCAGAA GAGCTTACGT GGGAACGAAT GCTAGGACTC 
GATGGATCAC TAGTTTTTCT AGAACATGTC TTTTGGGTGG TGTCTTTAAA CACATTGTTC 
ATTCTCGTTT TTGCATTTTG CCCTTACCAC ATCGGTCACT TTTCCCTTGT TGGTTTGGGA 
TTTGAAGAAC ACGTCCAAGC ATCTCATTTT GAAGGCCTAA TCACAACCAT AGTTGGGTAT 
ATACTTTTAG CAATAACATT GATAATTTGT CATGGGTTGG CAACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG AGTCTGCTAT ATTGTTGTGA AGGTCTCTTT GTTAGTGGTG 
GTAGAAATTG GAGTTTTCCC TCTCATTTGT GGCTGGTGGC TGGATATCTG TTCCTTGGAA 
ATGTTTGACG CTACTCTGAA AGATCGGGAG CTGAGCTTTC AGTCGGCCCC AGGCACTACC 
ATGTTCCTGC ACTGGCTGGT GGGAATGGTG TATGTGTTCT ACTTTGCCTC CTTCATTCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTT TTATGGTTTC TAAGGAATTT GAATGATCCA 
GATTTTAATC CAGTACAAGA AATGATTCAC TTGCCTATTT ATAGGCATCT TCGAAGATTT 
ATTTTGTCAG TGATTGTCTT CGGCTCCATC GTACTCCTAA TGCTGTGGCT GCCTATACGG 
ATAATCAAGA GTCTGCTGCC TCATTTTCTT CCATACAACG TCATGCTCTA TAGTGATGCT 
CCAGTGAGTG AACTGTCCCT TGAGCTGCTT CTGCTTCAGG TTGTCTTACC AGCGTTACTC 
GAACAGGGAC ACACGCGGCA GTGGCTGAAG GGACTGGTGC GAGCCTGGAC TGTCACTGCT 
GGATACTTGC TGGATCTTCA TTCCTACTTG TTGGGAGACC AGGAAGAAAA TGAGAACAGT 
GCAAACCAAC AGGTTAACAA TAACCAGCAT GCTCGAAATA ACAACGCTAT TCCCGTGGTA 
GGGGAAGGCC TGCACGCAGC CCACCAAGCC ATACTCCAGC AGGGCGGGCC TGTTGGCTTT 
CAGCCTTACC GCCGGCCTTT AAATTTTCCA CTCAGGATAT TTTTGTTGAT TGTCTTCATG 
TGTATAACCT TACTGATTGC CAGCCTCATC TGCCTTACTC TACCAGTATT TGCTGGCCGT 
TGGTTAATGT CATTTTGGAC CGGGACTGCC AAAATCCATG AGCTCTACAC AGCTGCCTGT 
GGTCTCTATG TTTGCTGGCT CACCATCCGG GCGGTGACGG TACTGGTGGC GTGGATGCCA 
CAGGGACGCA GAGTGATCTT CCAGAAGGTT AAGGAGTGGT CCCTCATGAT AATGAAGACT 
GTGATCGTGG CAGTGCTGCT GGCTGGGGTG GTCCCGCTTC TTCTGGGGCT TCTGTTTGAG 
CTGGTTATTG TTGCTCCCCT GAGGGTTCCT TTGGATCAGA CTCCTCTTTT TTACCCATGG 
CAGGACTGGG CACTTGGAGT CCTGCATGCC AAAATCATTG CAGCTATAAC ATTGATGGGT 
CCTCAGTGGT GGCTGAAAAC TGTAATTGAG CAGGTTTACG CAAATGGCAT CCGGAACATT 
GACCTTCAGT ACATCGTTCG GAAACTGGCA GCGCCAGTGA TCTCTGTGCT CTTGCTTTCC 
CTGTGTGTAC CTTACGTCAT CGCGTCCGGC ATCGTTCCTT TACTCGGTGT TACTGCGGAA 
ATGCAAAATT TGGTCCATCG GCGGATTTAT CCATTTTTGC TGATGGTTGT GGTGTTGATG 
GGAATCTTGT CCTTCCAGGT CCGCCAGTTT AAGCGCCTTT ATGAACATAT TAAAAATGAC 
AAGTACCTTG TGGGCCAGCG CCTCGTGAAC TATGAACGGA AATCCGGCAA ACAAGGCACA 
TCACCACCAC CTCCACAGTC GTCCCAAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192741.1
CDS	12..2744
Translation	MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTDNLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPAGGNGAENVAPEQPANPPAENAVVGENPDAQDEQAEEEEEENEEEDDAGGEDAADANNGVQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHGLATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSLLPHFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQHARNNNAIPVVGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVLVAWMPQGRRVIFQKVKEWSLMIMKTVIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLQYIVRKLAAPVISVLLLSLCVPYVIASGIVPLLGVTAEMQNLVHRRIYPFLLMVVVLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGTSPPPPQSSQE

Target ORF information:

RefSeq Version	NM_001205812.3
Organism	Bos taurus(cattle)
Definition	Bos taurus membrane associated ring-CH-type finger 6 (MARCHF6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205812.3

ORF Insert Sequence:

ATGGACACCG CGGAGGAAGA CATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTATC ATCCTTGTGT GTGTACTGGC AGTATTAAGT TTATCCATCA AGAATGCTTA 
GTTCAGTGGC TGAAACACAG TCGAAAAGAA TACTGTGAAT TATGCAAGCA CAGGTTTGCT 
TTCACACCAA TTTATTCCCC AGATATGCCT TCACGGCTTC CAATCCAGGA CATATTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCAATA CGATATTGGT TTCATTATAC ACTTGTGGCC 
TTTGCATGGT TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCTGTGA GCTCCCTCCT GACGCTGCCG CTGGACATGC TGTCCACGGA CAACTTGTTG 
GCGGATTGTT TGCAGGGTTG TTTTGTGGTG ACGTGCACAC TGTGCGCGTT CATCAGCCTG 
GTGTGGCTGC GAGAGCAGAT CGTCCACGGG GGAGCCCCAA TCTGGCTGGA GCATGCCGCT 
CCGCCCTTCA ATGCTGCTGG GCATCACCAA AATGAGGCTC CAGCTGGAGG AAATGGTGCT 
GAAAATGTTG CCCCCGAGCA GCCTGCTAAC CCCCCAGCAG AGAACGCGGT GGTGGGGGAA 
AACCCTGATG CCCAGGATGA GCAGGCGGAG GAGGAGGAGG AGGAGAACGA GGAGGAAGAC 
GACGCGGGCG GGGAGGATGC CGCTGACGCC AACAACGGGG TGCAGGATGA CATGAACTGG 
AATGCTTTAG AATGGGACCG AGCTGCAGAA GAGCTTACGT GGGAACGAAT GCTAGGACTC 
GATGGATCAC TAGTTTTTCT AGAACATGTC TTTTGGGTGG TGTCTTTAAA CACATTGTTC 
ATTCTCGTTT TTGCATTTTG CCCTTACCAC ATCGGTCACT TTTCCCTTGT TGGTTTGGGA 
TTTGAAGAAC ACGTCCAAGC ATCTCATTTT GAAGGCCTAA TCACAACCAT AGTTGGGTAT 
ATACTTTTAG CAATAACATT GATAATTTGT CATGGGTTGG CAACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG AGTCTGCTAT ATTGTTGTGA AGGTCTCTTT GTTAGTGGTG 
GTAGAAATTG GAGTTTTCCC TCTCATTTGT GGCTGGTGGC TGGATATCTG TTCCTTGGAA 
ATGTTTGACG CTACTCTGAA AGATCGGGAG CTGAGCTTTC AGTCGGCCCC AGGCACTACC 
ATGTTCCTGC ACTGGCTGGT GGGAATGGTG TATGTGTTCT ACTTTGCCTC CTTCATTCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTT TTATGGTTTC TAAGGAATTT GAATGATCCA 
GATTTTAATC CAGTACAAGA AATGATTCAC TTGCCTATTT ATAGGCATCT TCGAAGATTT 
ATTTTGTCAG TGATTGTCTT CGGCTCCATC GTACTCCTAA TGCTGTGGCT GCCTATACGG 
ATAATCAAGA GTCTGCTGCC TCATTTTCTT CCATACAACG TCATGCTCTA TAGTGATGCT 
CCAGTGAGTG AACTGTCCCT TGAGCTGCTT CTGCTTCAGG TTGTCTTACC AGCGTTACTC 
GAACAGGGAC ACACGCGGCA GTGGCTGAAG GGACTGGTGC GAGCCTGGAC TGTCACTGCT 
GGATACTTGC TGGATCTTCA TTCCTACTTG TTGGGAGACC AGGAAGAAAA TGAGAACAGT 
GCAAACCAAC AGGTTAACAA TAACCAGCAT GCTCGAAATA ACAACGCTAT TCCCGTGGTA 
GGGGAAGGCC TGCACGCAGC CCACCAAGCC ATACTCCAGC AGGGCGGGCC TGTTGGCTTT 
CAGCCTTACC GCCGGCCTTT AAATTTTCCA CTCAGGATAT TTTTGTTGAT TGTCTTCATG 
TGTATAACCT TACTGATTGC CAGCCTCATC TGCCTTACTC TACCAGTATT TGCTGGCCGT 
TGGTTAATGT CATTTTGGAC CGGGACTGCC AAAATCCATG AGCTCTACAC AGCTGCCTGT 
GGTCTCTATG TTTGCTGGCT CACCATCCGG GCGGTGACGG TACTGGTGGC GTGGATGCCA 
CAGGGACGCA GAGTGATCTT CCAGAAGGTT AAGGAGTGGT CCCTCATGAT AATGAAGACT 
GTGATCGTGG CAGTGCTGCT GGCTGGGGTG GTCCCGCTTC TTCTGGGGCT TCTGTTTGAG 
CTGGTTATTG TTGCTCCCCT GAGGGTTCCT TTGGATCAGA CTCCTCTTTT TTACCCATGG 
CAGGACTGGG CACTTGGAGT CCTGCATGCC AAAATCATTG CAGCTATAAC ATTGATGGGT 
CCTCAGTGGT GGCTGAAAAC TGTAATTGAG CAGGTTTACG CAAATGGCAT CCGGAACATT 
GACCTTCAGT ACATCGTTCG GAAACTGGCA GCGCCAGTGA TCTCTGTGCT CTTGCTTTCC 
CTGTGTGTAC CTTACGTCAT CGCGTCCGGC ATCGTTCCTT TACTCGGTGT TACTGCGGAA 
ATGCAAAATT TGGTCCATCG GCGGATTTAT CCATTTTTGC TGATGGTTGT GGTGTTGATG 
GGAATCTTGT CCTTCCAGGT CCGCCAGTTT AAGCGCCTTT ATGAACATAT TAAAAATGAC 
AAGTACCTTG TGGGCCAGCG CCTCGTGAAC TATGAACGGA AATCCGGCAA ACAAGGCACA 
TCACCACCAC CTCCACAGTC GTCCCAAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARCHF6 ( XM_024981357.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837125.1 Bos taurus membrane associated ring-CH-type finger 6 (MARCH6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa228061
Clone ID Related Accession (Same CDS sequence)	XM_024981357.1 , XM_024981358.1
Accession Version	XM_024981357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase MARCH6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCAC GGCTTCCAAT CCAGGACATA TTTGCTGGAC TGGTTACAAG TATTGGCACT 
GCAATACGAT ATTGGTTTCA TTATACACTT GTGGCCTTTG CATGGTTGGG AGTTGTTCCT 
CTTACAGCAT GCCGCATCTA CAAGTGCTTG TTTACTGGCT CTGTGAGCTC CCTCCTGACG 
CTGCCGCTGG ACATGCTGTC CACGGACAAC TTGTTGGCGG ATTGTTTGCA GGGTTGTTTT 
GTGGTGACGT GCACACTGTG CGCGTTCATC AGCCTGGTGT GGCTGCGAGA GCAGATCGTC 
CACGGGGGAG CCCCAATCTG GCTGGAGCAT GCCGCTCCGC CCTTCAATGC TGCTGGGCAT 
CACCAAAATG AGGCTCCAGC TGGAGGAAAT GGTGCTGAAA ATGTTGCCCC CGAGCAGCCT 
GCTAACCCCC CAGCAGAGAA CGCGGTGGTG GGGGAAAACC CTGATGCCCA GGATGAGCAG 
GCGGAGGAGG AGGAGGAGGA GAACGAGGAG GAAGACGACG CGGGCGGGGA GGATGCCGCT 
GACGCCAACA ACGGGGTGCA GGATGACATG AACTGGAATG CTTTAGAATG GGACCGAGCT 
GCAGAAGAGC TTACGTGGGA ACGAATGCTA GGACTCGATG GATCACTAGT TTTTCTAGAA 
CATGTCTTTT GGGTGGTGTC TTTAAACACA TTGTTCATTC TCGTTTTTGC ATTTTGCCCT 
TACCACATCG GTCACTTTTC CCTTGTTGGT TTGGGATTTG AAGAACACGT CCAAGCATCT 
CATTTTGAAG GCCTAATCAC AACCATAGTT GGGTATATAC TTTTAGCAAT AACATTGATA 
ATTTGTCATG GGTTGGCAAC TCTTGTTAAA TTTCATAGGT CTCGTCGCTT GCTGGGAGTC 
TGCTATATTG TTGTGAAGGT CTCTTTGTTA GTGGTGGTAG AAATTGGAGT TTTCCCTCTC 
ATTTGTGGCT GGTGGCTGGA TATCTGTTCC TTGGAAATGT TTGACGCTAC TCTGAAAGAT 
CGGGAGCTGA GCTTTCAGTC GGCCCCAGGC ACTACCATGT TCCTGCACTG GCTGGTGGGA 
ATGGTGTATG TGTTCTACTT TGCCTCCTTC ATTCTGTTAC TGAGAGAGGT ACTTCGACCT 
GGTGTTTTAT GGTTTCTAAG GAATTTGAAT GATCCAGATT TTAATCCAGT ACAAGAAATG 
ATTCACTTGC CTATTTATAG GCATCTTCGA AGATTTATTT TGTCAGTGAT TGTCTTCGGC 
TCCATCGTAC TCCTAATGCT GTGGCTGCCT ATACGGATAA TCAAGAGTCT GCTGCCTCAT 
TTTCTTCCAT ACAACGTCAT GCTCTATAGT GATGCTCCAG TGAGTGAACT GTCCCTTGAG 
CTGCTTCTGC TTCAGGTTGT CTTACCAGCG TTACTCGAAC AGGGACACAC GCGGCAGTGG 
CTGAAGGGAC TGGTGCGAGC CTGGACTGTC ACTGCTGGAT ACTTGCTGGA TCTTCATTCC 
TACTTGTTGG GAGACCAGGA AGAAAATGAG AACAGTGCAA ACCAACAGGT TAACAATAAC 
CAGCATGCTC GAAATAACAA CGCTATTCCC GTGGTAGGGG AAGGCCTGCA CGCAGCCCAC 
CAAGCCATAC TCCAGCAGGG CGGGCCTGTT GGCTTTCAGC CTTACCGCCG GCCTTTAAAT 
TTTCCACTCA GGATATTTTT GTTGATTGTC TTCATGTGTA TAACCTTACT GATTGCCAGC 
CTCATCTGCC TTACTCTACC AGTATTTGCT GGCCGTTGGT TAATGTCATT TTGGACCGGG 
ACTGCCAAAA TCCATGAGCT CTACACAGCT GCCTGTGGTC TCTATGTTTG CTGGCTCACC 
ATCCGGGCGG TGACGGTACT GGTGGCGTGG ATGCCACAGG GACGCAGAGT GATCTTCCAG 
AAGGTTAAGG AGTGGTCCCT CATGATAATG AAGACTGTGA TCGTGGCAGT GCTGCTGGCT 
GGGGTGGTCC CGCTTCTTCT GGGGCTTCTG TTTGAGCTGG TTATTGTTGC TCCCCTGAGG 
GTTCCTTTGG ATCAGACTCC TCTTTTTTAC CCATGGCAGG ACTGGGCACT TGGAGTCCTG 
CATGCCAAAA TCATTGCAGC TATAACATTG ATGGGTCCTC AGTGGTGGCT GAAAACTGTA 
ATTGAGCAGG TTTACGCAAA TGGCATCCGG AACATTGACC TTCAGTACAT CGTTCGGAAA 
CTGGCAGCGC CAGTGATCTC TGTGCTCTTG CTTTCCCTGT GTGTACCTTA CGTCATCGCG 
TCCGGCATCG TTCCTTTACT CGGTGTTACT GCGGAAATGC AAAATTTGGT CCATCGGCGG 
ATTTATCCAT TTTTGCTGAT GGTTGTGGTG TTGATGGGAA TCTTGTCCTT CCAGGTCCGC 
CAGTTTAAGC GCCTTTATGA ACATATTAAA AATGACAAGT ACCTTGTGGG CCAGCGCCTC 
GTGAACTATG AACGGAAATC CGGCAAACAA GGCACATCAC CACCACCTCC ACAGTCGTCC 
CAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837125.1
CDS	491..3019
Translation	MPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTDNLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPAGGNGAENVAPEQPANPPAENAVVGENPDAQDEQAEEEEEENEEEDDAGGEDAADANNGVQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHGLATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSLLPHFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQHARNNNAIPVVGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVLVAWMPQGRRVIFQKVKEWSLMIMKTVIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLQYIVRKLAAPVISVLLLSLCVPYVIASGIVPLLGVTAEMQNLVHRRIYPFLLMVVVLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGTSPPPPQSSQE

Target ORF information:

RefSeq Version	XM_024981357.1
Organism	Bos taurus(cattle)
Definition	Bos taurus membrane associated ring-CH-type finger 6 (MARCH6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981357.1

ORF Insert Sequence:

ATGCCTTCAC GGCTTCCAAT CCAGGACATA TTTGCTGGAC TGGTTACAAG TATTGGCACT 
GCAATACGAT ATTGGTTTCA TTATACACTT GTGGCCTTTG CATGGTTGGG AGTTGTTCCT 
CTTACAGCAT GCCGCATCTA CAAGTGCTTG TTTACTGGCT CTGTGAGCTC CCTCCTGACG 
CTGCCGCTGG ACATGCTGTC CACGGACAAC TTGTTGGCGG ATTGTTTGCA GGGTTGTTTT 
GTGGTGACGT GCACACTGTG CGCGTTCATC AGCCTGGTGT GGCTGCGAGA GCAGATCGTC 
CACGGGGGAG CCCCAATCTG GCTGGAGCAT GCCGCTCCGC CCTTCAATGC TGCTGGGCAT 
CACCAAAATG AGGCTCCAGC TGGAGGAAAT GGTGCTGAAA ATGTTGCCCC CGAGCAGCCT 
GCTAACCCCC CAGCAGAGAA CGCGGTGGTG GGGGAAAACC CTGATGCCCA GGATGAGCAG 
GCGGAGGAGG AGGAGGAGGA GAACGAGGAG GAAGACGACG CGGGCGGGGA GGATGCCGCT 
GACGCCAACA ACGGGGTGCA GGATGACATG AACTGGAATG CTTTAGAATG GGACCGAGCT 
GCAGAAGAGC TTACGTGGGA ACGAATGCTA GGACTCGATG GATCACTAGT TTTTCTAGAA 
CATGTCTTTT GGGTGGTGTC TTTAAACACA TTGTTCATTC TCGTTTTTGC ATTTTGCCCT 
TACCACATCG GTCACTTTTC CCTTGTTGGT TTGGGATTTG AAGAACACGT CCAAGCATCT 
CATTTTGAAG GCCTAATCAC AACCATAGTT GGGTATATAC TTTTAGCAAT AACATTGATA 
ATTTGTCATG GGTTGGCAAC TCTTGTTAAA TTTCATAGGT CTCGTCGCTT GCTGGGAGTC 
TGCTATATTG TTGTGAAGGT CTCTTTGTTA GTGGTGGTAG AAATTGGAGT TTTCCCTCTC 
ATTTGTGGCT GGTGGCTGGA TATCTGTTCC TTGGAAATGT TTGACGCTAC TCTGAAAGAT 
CGGGAGCTGA GCTTTCAGTC GGCCCCAGGC ACTACCATGT TCCTGCACTG GCTGGTGGGA 
ATGGTGTATG TGTTCTACTT TGCCTCCTTC ATTCTGTTAC TGAGAGAGGT ACTTCGACCT 
GGTGTTTTAT GGTTTCTAAG GAATTTGAAT GATCCAGATT TTAATCCAGT ACAAGAAATG 
ATTCACTTGC CTATTTATAG GCATCTTCGA AGATTTATTT TGTCAGTGAT TGTCTTCGGC 
TCCATCGTAC TCCTAATGCT GTGGCTGCCT ATACGGATAA TCAAGAGTCT GCTGCCTCAT 
TTTCTTCCAT ACAACGTCAT GCTCTATAGT GATGCTCCAG TGAGTGAACT GTCCCTTGAG 
CTGCTTCTGC TTCAGGTTGT CTTACCAGCG TTACTCGAAC AGGGACACAC GCGGCAGTGG 
CTGAAGGGAC TGGTGCGAGC CTGGACTGTC ACTGCTGGAT ACTTGCTGGA TCTTCATTCC 
TACTTGTTGG GAGACCAGGA AGAAAATGAG AACAGTGCAA ACCAACAGGT TAACAATAAC 
CAGCATGCTC GAAATAACAA CGCTATTCCC GTGGTAGGGG AAGGCCTGCA CGCAGCCCAC 
CAAGCCATAC TCCAGCAGGG CGGGCCTGTT GGCTTTCAGC CTTACCGCCG GCCTTTAAAT 
TTTCCACTCA GGATATTTTT GTTGATTGTC TTCATGTGTA TAACCTTACT GATTGCCAGC 
CTCATCTGCC TTACTCTACC AGTATTTGCT GGCCGTTGGT TAATGTCATT TTGGACCGGG 
ACTGCCAAAA TCCATGAGCT CTACACAGCT GCCTGTGGTC TCTATGTTTG CTGGCTCACC 
ATCCGGGCGG TGACGGTACT GGTGGCGTGG ATGCCACAGG GACGCAGAGT GATCTTCCAG 
AAGGTTAAGG AGTGGTCCCT CATGATAATG AAGACTGTGA TCGTGGCAGT GCTGCTGGCT 
GGGGTGGTCC CGCTTCTTCT GGGGCTTCTG TTTGAGCTGG TTATTGTTGC TCCCCTGAGG 
GTTCCTTTGG ATCAGACTCC TCTTTTTTAC CCATGGCAGG ACTGGGCACT TGGAGTCCTG 
CATGCCAAAA TCATTGCAGC TATAACATTG ATGGGTCCTC AGTGGTGGCT GAAAACTGTA 
ATTGAGCAGG TTTACGCAAA TGGCATCCGG AACATTGACC TTCAGTACAT CGTTCGGAAA 
CTGGCAGCGC CAGTGATCTC TGTGCTCTTG CTTTCCCTGT GTGTACCTTA CGTCATCGCG 
TCCGGCATCG TTCCTTTACT CGGTGTTACT GCGGAAATGC AAAATTTGGT CCATCGGCGG 
ATTTATCCAT TTTTGCTGAT GGTTGTGGTG TTGATGGGAA TCTTGTCCTT CCAGGTCCGC 
CAGTTTAAGC GCCTTTATGA ACATATTAAA AATGACAAGT ACCTTGTGGG CCAGCGCCTC 
GTGAACTATG AACGGAAATC CGGCAAACAA GGCACATCAC CACCACCTCC ACAGTCGTCC 
CAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARCHF6 ( XM_024981358.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837126.1 Bos taurus membrane associated ring-CH-type finger 6 (MARCH6), transcript variant X2, mRNA.

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CloneID	OBa228061
Clone ID Related Accession (Same CDS sequence)	XM_024981357.1 , XM_024981358.1
Accession Version	XM_024981358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase MARCH6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCAC GGCTTCCAAT CCAGGACATA TTTGCTGGAC TGGTTACAAG TATTGGCACT 
GCAATACGAT ATTGGTTTCA TTATACACTT GTGGCCTTTG CATGGTTGGG AGTTGTTCCT 
CTTACAGCAT GCCGCATCTA CAAGTGCTTG TTTACTGGCT CTGTGAGCTC CCTCCTGACG 
CTGCCGCTGG ACATGCTGTC CACGGACAAC TTGTTGGCGG ATTGTTTGCA GGGTTGTTTT 
GTGGTGACGT GCACACTGTG CGCGTTCATC AGCCTGGTGT GGCTGCGAGA GCAGATCGTC 
CACGGGGGAG CCCCAATCTG GCTGGAGCAT GCCGCTCCGC CCTTCAATGC TGCTGGGCAT 
CACCAAAATG AGGCTCCAGC TGGAGGAAAT GGTGCTGAAA ATGTTGCCCC CGAGCAGCCT 
GCTAACCCCC CAGCAGAGAA CGCGGTGGTG GGGGAAAACC CTGATGCCCA GGATGAGCAG 
GCGGAGGAGG AGGAGGAGGA GAACGAGGAG GAAGACGACG CGGGCGGGGA GGATGCCGCT 
GACGCCAACA ACGGGGTGCA GGATGACATG AACTGGAATG CTTTAGAATG GGACCGAGCT 
GCAGAAGAGC TTACGTGGGA ACGAATGCTA GGACTCGATG GATCACTAGT TTTTCTAGAA 
CATGTCTTTT GGGTGGTGTC TTTAAACACA TTGTTCATTC TCGTTTTTGC ATTTTGCCCT 
TACCACATCG GTCACTTTTC CCTTGTTGGT TTGGGATTTG AAGAACACGT CCAAGCATCT 
CATTTTGAAG GCCTAATCAC AACCATAGTT GGGTATATAC TTTTAGCAAT AACATTGATA 
ATTTGTCATG GGTTGGCAAC TCTTGTTAAA TTTCATAGGT CTCGTCGCTT GCTGGGAGTC 
TGCTATATTG TTGTGAAGGT CTCTTTGTTA GTGGTGGTAG AAATTGGAGT TTTCCCTCTC 
ATTTGTGGCT GGTGGCTGGA TATCTGTTCC TTGGAAATGT TTGACGCTAC TCTGAAAGAT 
CGGGAGCTGA GCTTTCAGTC GGCCCCAGGC ACTACCATGT TCCTGCACTG GCTGGTGGGA 
ATGGTGTATG TGTTCTACTT TGCCTCCTTC ATTCTGTTAC TGAGAGAGGT ACTTCGACCT 
GGTGTTTTAT GGTTTCTAAG GAATTTGAAT GATCCAGATT TTAATCCAGT ACAAGAAATG 
ATTCACTTGC CTATTTATAG GCATCTTCGA AGATTTATTT TGTCAGTGAT TGTCTTCGGC 
TCCATCGTAC TCCTAATGCT GTGGCTGCCT ATACGGATAA TCAAGAGTCT GCTGCCTCAT 
TTTCTTCCAT ACAACGTCAT GCTCTATAGT GATGCTCCAG TGAGTGAACT GTCCCTTGAG 
CTGCTTCTGC TTCAGGTTGT CTTACCAGCG TTACTCGAAC AGGGACACAC GCGGCAGTGG 
CTGAAGGGAC TGGTGCGAGC CTGGACTGTC ACTGCTGGAT ACTTGCTGGA TCTTCATTCC 
TACTTGTTGG GAGACCAGGA AGAAAATGAG AACAGTGCAA ACCAACAGGT TAACAATAAC 
CAGCATGCTC GAAATAACAA CGCTATTCCC GTGGTAGGGG AAGGCCTGCA CGCAGCCCAC 
CAAGCCATAC TCCAGCAGGG CGGGCCTGTT GGCTTTCAGC CTTACCGCCG GCCTTTAAAT 
TTTCCACTCA GGATATTTTT GTTGATTGTC TTCATGTGTA TAACCTTACT GATTGCCAGC 
CTCATCTGCC TTACTCTACC AGTATTTGCT GGCCGTTGGT TAATGTCATT TTGGACCGGG 
ACTGCCAAAA TCCATGAGCT CTACACAGCT GCCTGTGGTC TCTATGTTTG CTGGCTCACC 
ATCCGGGCGG TGACGGTACT GGTGGCGTGG ATGCCACAGG GACGCAGAGT GATCTTCCAG 
AAGGTTAAGG AGTGGTCCCT CATGATAATG AAGACTGTGA TCGTGGCAGT GCTGCTGGCT 
GGGGTGGTCC CGCTTCTTCT GGGGCTTCTG TTTGAGCTGG TTATTGTTGC TCCCCTGAGG 
GTTCCTTTGG ATCAGACTCC TCTTTTTTAC CCATGGCAGG ACTGGGCACT TGGAGTCCTG 
CATGCCAAAA TCATTGCAGC TATAACATTG ATGGGTCCTC AGTGGTGGCT GAAAACTGTA 
ATTGAGCAGG TTTACGCAAA TGGCATCCGG AACATTGACC TTCAGTACAT CGTTCGGAAA 
CTGGCAGCGC CAGTGATCTC TGTGCTCTTG CTTTCCCTGT GTGTACCTTA CGTCATCGCG 
TCCGGCATCG TTCCTTTACT CGGTGTTACT GCGGAAATGC AAAATTTGGT CCATCGGCGG 
ATTTATCCAT TTTTGCTGAT GGTTGTGGTG TTGATGGGAA TCTTGTCCTT CCAGGTCCGC 
CAGTTTAAGC GCCTTTATGA ACATATTAAA AATGACAAGT ACCTTGTGGG CCAGCGCCTC 
GTGAACTATG AACGGAAATC CGGCAAACAA GGCACATCAC CACCACCTCC ACAGTCGTCC 
CAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837126.1
CDS	452..2980
Translation	MPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTDNLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPAGGNGAENVAPEQPANPPAENAVVGENPDAQDEQAEEEEEENEEEDDAGGEDAADANNGVQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHGLATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSLLPHFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQHARNNNAIPVVGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVLVAWMPQGRRVIFQKVKEWSLMIMKTVIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLQYIVRKLAAPVISVLLLSLCVPYVIASGIVPLLGVTAEMQNLVHRRIYPFLLMVVVLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGTSPPPPQSSQE

Target ORF information:

RefSeq Version	XM_024981358.1
Organism	Bos taurus(cattle)
Definition	Bos taurus membrane associated ring-CH-type finger 6 (MARCH6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981358.1

ORF Insert Sequence:

ATGCCTTCAC GGCTTCCAAT CCAGGACATA TTTGCTGGAC TGGTTACAAG TATTGGCACT 
GCAATACGAT ATTGGTTTCA TTATACACTT GTGGCCTTTG CATGGTTGGG AGTTGTTCCT 
CTTACAGCAT GCCGCATCTA CAAGTGCTTG TTTACTGGCT CTGTGAGCTC CCTCCTGACG 
CTGCCGCTGG ACATGCTGTC CACGGACAAC TTGTTGGCGG ATTGTTTGCA GGGTTGTTTT 
GTGGTGACGT GCACACTGTG CGCGTTCATC AGCCTGGTGT GGCTGCGAGA GCAGATCGTC 
CACGGGGGAG CCCCAATCTG GCTGGAGCAT GCCGCTCCGC CCTTCAATGC TGCTGGGCAT 
CACCAAAATG AGGCTCCAGC TGGAGGAAAT GGTGCTGAAA ATGTTGCCCC CGAGCAGCCT 
GCTAACCCCC CAGCAGAGAA CGCGGTGGTG GGGGAAAACC CTGATGCCCA GGATGAGCAG 
GCGGAGGAGG AGGAGGAGGA GAACGAGGAG GAAGACGACG CGGGCGGGGA GGATGCCGCT 
GACGCCAACA ACGGGGTGCA GGATGACATG AACTGGAATG CTTTAGAATG GGACCGAGCT 
GCAGAAGAGC TTACGTGGGA ACGAATGCTA GGACTCGATG GATCACTAGT TTTTCTAGAA 
CATGTCTTTT GGGTGGTGTC TTTAAACACA TTGTTCATTC TCGTTTTTGC ATTTTGCCCT 
TACCACATCG GTCACTTTTC CCTTGTTGGT TTGGGATTTG AAGAACACGT CCAAGCATCT 
CATTTTGAAG GCCTAATCAC AACCATAGTT GGGTATATAC TTTTAGCAAT AACATTGATA 
ATTTGTCATG GGTTGGCAAC TCTTGTTAAA TTTCATAGGT CTCGTCGCTT GCTGGGAGTC 
TGCTATATTG TTGTGAAGGT CTCTTTGTTA GTGGTGGTAG AAATTGGAGT TTTCCCTCTC 
ATTTGTGGCT GGTGGCTGGA TATCTGTTCC TTGGAAATGT TTGACGCTAC TCTGAAAGAT 
CGGGAGCTGA GCTTTCAGTC GGCCCCAGGC ACTACCATGT TCCTGCACTG GCTGGTGGGA 
ATGGTGTATG TGTTCTACTT TGCCTCCTTC ATTCTGTTAC TGAGAGAGGT ACTTCGACCT 
GGTGTTTTAT GGTTTCTAAG GAATTTGAAT GATCCAGATT TTAATCCAGT ACAAGAAATG 
ATTCACTTGC CTATTTATAG GCATCTTCGA AGATTTATTT TGTCAGTGAT TGTCTTCGGC 
TCCATCGTAC TCCTAATGCT GTGGCTGCCT ATACGGATAA TCAAGAGTCT GCTGCCTCAT 
TTTCTTCCAT ACAACGTCAT GCTCTATAGT GATGCTCCAG TGAGTGAACT GTCCCTTGAG 
CTGCTTCTGC TTCAGGTTGT CTTACCAGCG TTACTCGAAC AGGGACACAC GCGGCAGTGG 
CTGAAGGGAC TGGTGCGAGC CTGGACTGTC ACTGCTGGAT ACTTGCTGGA TCTTCATTCC 
TACTTGTTGG GAGACCAGGA AGAAAATGAG AACAGTGCAA ACCAACAGGT TAACAATAAC 
CAGCATGCTC GAAATAACAA CGCTATTCCC GTGGTAGGGG AAGGCCTGCA CGCAGCCCAC 
CAAGCCATAC TCCAGCAGGG CGGGCCTGTT GGCTTTCAGC CTTACCGCCG GCCTTTAAAT 
TTTCCACTCA GGATATTTTT GTTGATTGTC TTCATGTGTA TAACCTTACT GATTGCCAGC 
CTCATCTGCC TTACTCTACC AGTATTTGCT GGCCGTTGGT TAATGTCATT TTGGACCGGG 
ACTGCCAAAA TCCATGAGCT CTACACAGCT GCCTGTGGTC TCTATGTTTG CTGGCTCACC 
ATCCGGGCGG TGACGGTACT GGTGGCGTGG ATGCCACAGG GACGCAGAGT GATCTTCCAG 
AAGGTTAAGG AGTGGTCCCT CATGATAATG AAGACTGTGA TCGTGGCAGT GCTGCTGGCT 
GGGGTGGTCC CGCTTCTTCT GGGGCTTCTG TTTGAGCTGG TTATTGTTGC TCCCCTGAGG 
GTTCCTTTGG ATCAGACTCC TCTTTTTTAC CCATGGCAGG ACTGGGCACT TGGAGTCCTG 
CATGCCAAAA TCATTGCAGC TATAACATTG ATGGGTCCTC AGTGGTGGCT GAAAACTGTA 
ATTGAGCAGG TTTACGCAAA TGGCATCCGG AACATTGACC TTCAGTACAT CGTTCGGAAA 
CTGGCAGCGC CAGTGATCTC TGTGCTCTTG CTTTCCCTGT GTGTACCTTA CGTCATCGCG 
TCCGGCATCG TTCCTTTACT CGGTGTTACT GCGGAAATGC AAAATTTGGT CCATCGGCGG 
ATTTATCCAT TTTTGCTGAT GGTTGTGGTG TTGATGGGAA TCTTGTCCTT CCAGGTCCGC 
CAGTTTAAGC GCCTTTATGA ACATATTAAA AATGACAAGT ACCTTGTGGG CCAGCGCCTC 
GTGAACTATG AACGGAAATC CGGCAAACAA GGCACATCAC CACCACCTCC ACAGTCGTCC 
CAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MARCHF6 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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