GRIN2B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRIN2B
Entrez Gene ID	537804
Full Name	glutamate ionotropic receptor NMDA type subunit 2B
General protein information	Preferred Names glutamate ionotropic receptor NMDA type subunit 2B Names glutamate receptor ionotropic, NMDA 2B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001192921.1	NP_001179850.1	glutamate receptor ionotropic, NMDA 2B precursor
NM_001192921.2	NP_001179850.1	glutamate receptor ionotropic, NMDA 2B precursor
NM_001192921.3	NP_001179850.1	glutamate receptor ionotropic, NMDA 2B precursor
XM_005206988.3	XP_005207045.1	glutamate receptor ionotropic, NMDA 2B isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	GRIN2B	XP_001088140.1
Bos taurus (cattle)	GRIN2B	NP_001179850.1
Danio rerio (zebrafish)	LOC100334883	XP_003198060.2
Xenopus tropicalis (tropical clawed frog)	grin2b	XP_002932208.2
Pan troglodytes (chimpanzee)	GRIN2B	XP_528744.2
Canis lupus familiaris (dog)	GRIN2B	NP_001008719.1
Mus musculus (house mouse)	Grin2b	NP_032197.3
Danio rerio (zebrafish)	LOC559976	NP_001121809.1
Homo sapiens (human)	GRIN2B	NP_000825.2
Rattus norvegicus (Norway rat)	Grin2b	NP_036706.1
Gallus gallus (chicken)	GRIN2B	XP_416204.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GRIN2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRIN2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa19686	NM_001192921.2 Latest version!	Bos taurus glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa239137	NM_001192921.3 Latest version!	Bos taurus glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa36350	NM_001192921.1 Latest version!	Bos taurus glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa19686	XM_005206988.3 Latest version!	Bos taurus glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRIN2B ( NM_001192921.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179850.1 Bos taurus glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), mRNA.

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CloneID	OBa19686
Clone ID Related Accession (Same CDS sequence)	NM_001192921.2 , XM_005206988.3
Accession Version	NM_001192921.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1508601600000
Organism	Bos taurus(cattle)
Product	glutamate receptor ionotropic, NMDA 2B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02014253.1, DAAA02014255.1, DAAA02014257.1, DAAA02014262.1 and DAAA02014263.1. On Jun 30, 2017 this sequence version replaced NM_001192921.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN04019643 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC 
CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC 
CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC 
ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT 
GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC 
ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC 
TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC 
ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG 
GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG 
GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC 
AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC 
TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT 
CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG 
TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC 
ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC 
TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC 
AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA 
AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA 
GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG 
CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG 
AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC 
ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC 
TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG 
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG 
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC 
GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC 
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC 
ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT 
AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC 
ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG 
CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC 
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG 
GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT 
TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA 
GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC 
CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC 
GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT 
GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC 
CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC 
TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT 
GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC 
CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG 
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC 
CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT 
CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC 
GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC 
TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT 
GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG 
TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA 
GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG 
AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC 
AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG 
TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA 
CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC 
GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG 
CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG 
GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG 
GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT 
AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG 
GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC 
TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC 
CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG 
TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG 
GCCGTGACAT CCGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG 
AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA 
GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC 
GGCAGCCCCT ATGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC 
AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC 
GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC 
ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG 
CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC 
AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT 
ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC 
TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179850.1
CDS	1..4494
Translation	MKPRAECCSPKFWLVLAVLAVSGSRARSQKSPPSIGIAVILVGTSDEVAIKDAHEKDDFHHLSVVPRVELVAMNETDPKSIITRICDLMSDRKIQGVVFADDTDQEAIAQILDFISAQTLTPILGIHGGSSMIMADKDESSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLDDGDSKIQNQLKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPSEFPTGLVSVSYDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLINVTFEGRNLSFSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETEEQEDDHLSIVTLEEAPFVIVESVDPLSGTCMRNTVPCQKRIISENKTDEEPGYIKKCCKGFCIDILKKISKSVKFTYDLYLVTNGKHGKKINGTWNGMIGEVVMKRAYMAVGSLTINEERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYAEMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAIQKDSGWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDIDNMAGVFYMLGAAMALSLITFICEHLFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMANLSGVNGSPQSALDFIRRESSVYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNVYQDHYHHHHRPHSIGSASSIDGLYDCDNPPFTTQPRALGKKPLDIGLPSSKHSQLGDLYGKFSFKSDRYGGHDDLIRSDVSDISTHTVTYGNIEGNAAKRRKQQYKDSLKKRPASAKSRREFDEIELAYRRRPPRSPDHKRYFRDKDGLRDFYLDQFRAKETSPHWEHVDLTDLYKERGEDFKRDSVGGGGPCTNRAHHLKHGTGDKHGGGVVGGVPAPWEKNLTNVDWEERPGGNFCRSCPSKLHNYSTTTAATAVVAGQNSGRPACIRCEACKKAGNLYDISEDNSLQELDQPAAPVAAVTSAASKYPQSPTNSKAQKKPRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRFLDGSPYAHMFEMPAGESAFSGSNDGKAAAPAAGHHHHGNPGGGGGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHGSKSYFFRQPTVAGAPKARPDFRALVSNKPVVSALHGAVPGRFQKDICIGNQSNPCVPNNKNPRAFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	NM_001192921.2
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192921.2

ORF Insert Sequence:

ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC 
CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC 
CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC 
ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT 
GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC 
ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC 
TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC 
ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG 
GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG 
GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC 
AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC 
TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT 
CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG 
TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC 
ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC 
TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC 
AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA 
AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA 
GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG 
CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG 
AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC 
ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC 
TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG 
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG 
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC 
GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC 
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC 
ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT 
AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC 
ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG 
CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC 
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG 
GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT 
TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA 
GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC 
CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC 
GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT 
GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC 
CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC 
TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT 
GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC 
CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG 
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC 
CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT 
CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC 
GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC 
TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT 
GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG 
TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA 
GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG 
AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC 
AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG 
TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA 
CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC 
GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG 
CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG 
GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG 
GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT 
AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG 
GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC 
TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC 
CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG 
TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG 
GCCGTGACAT CCGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG 
AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA 
GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC 
GGCAGCCCCT ATGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC 
AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC 
GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC 
ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG 
CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC 
AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT 
ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC 
TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIN2B ( NM_001192921.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179850.1 Bos taurus glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), mRNA.

Price & Availability↑

CloneID	OBa239137
Clone ID Related Accession (Same CDS sequence)	NM_001192921.3
Accession Version	NM_001192921.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527523200000
Organism	Bos taurus(cattle)
Product	glutamate receptor ionotropic, NMDA 2B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000005.1. On May 30, 2018 this sequence version replaced NM_001192921.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145419, SAMN03145422 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC 
CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC 
CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC 
ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT 
GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC 
ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC 
TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC 
ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG 
GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG 
GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC 
AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC 
TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT 
CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG 
TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC 
ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC 
TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC 
AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA 
AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA 
GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG 
CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG 
AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC 
ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC 
TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG 
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG 
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC 
GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC 
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC 
ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT 
AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC 
ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG 
CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC 
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG 
GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT 
TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA 
GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC 
CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC 
GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT 
GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC 
CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC 
TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT 
GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC 
CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG 
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC 
CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT 
CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC 
GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC 
TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT 
GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG 
TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA 
GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG 
AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC 
AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG 
TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA 
CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC 
GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG 
CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG 
GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG 
GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT 
AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG 
GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC 
TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC 
CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG 
TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG 
GCCGTGACAT CTGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG 
AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA 
GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC 
GGCAGCCCCT ACGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC 
AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC 
GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC 
ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG 
CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC 
AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT 
ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC 
TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179850.1
CDS	1..4494
Translation	MKPRAECCSPKFWLVLAVLAVSGSRARSQKSPPSIGIAVILVGTSDEVAIKDAHEKDDFHHLSVVPRVELVAMNETDPKSIITRICDLMSDRKIQGVVFADDTDQEAIAQILDFISAQTLTPILGIHGGSSMIMADKDESSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLDDGDSKIQNQLKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPSEFPTGLVSVSYDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLINVTFEGRNLSFSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETEEQEDDHLSIVTLEEAPFVIVESVDPLSGTCMRNTVPCQKRIISENKTDEEPGYIKKCCKGFCIDILKKISKSVKFTYDLYLVTNGKHGKKINGTWNGMIGEVVMKRAYMAVGSLTINEERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYAEMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAIQKDSGWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDIDNMAGVFYMLGAAMALSLITFICEHLFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMANLSGVNGSPQSALDFIRRESSVYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNVYQDHYHHHHRPHSIGSASSIDGLYDCDNPPFTTQPRALGKKPLDIGLPSSKHSQLGDLYGKFSFKSDRYGGHDDLIRSDVSDISTHTVTYGNIEGNAAKRRKQQYKDSLKKRPASAKSRREFDEIELAYRRRPPRSPDHKRYFRDKDGLRDFYLDQFRAKETSPHWEHVDLTDLYKERGEDFKRDSVGGGGPCTNRAHHLKHGTGDKHGGGVVGGVPAPWEKNLTNVDWEERPGGNFCRSCPSKLHNYSTTTAATAVVAGQNSGRPACIRCEACKKAGNLYDISEDNSLQELDQPAAPVAAVTSAASKYPQSPTNSKAQKKPRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRFLDGSPYAHMFEMPAGESAFSGSNDGKAAAPAAGHHHHGNPGGGGGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHGSKSYFFRQPTVAGAPKARPDFRALVSNKPVVSALHGAVPGRFQKDICIGNQSNPCVPNNKNPRAFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	NM_001192921.3
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192921.3

ORF Insert Sequence:

ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC 
CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC 
CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC 
ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT 
GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC 
ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC 
TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC 
ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG 
GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG 
GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC 
AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC 
TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT 
CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG 
TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC 
ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC 
TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC 
AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA 
AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA 
GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG 
CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG 
AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC 
ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC 
TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG 
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG 
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC 
GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC 
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC 
ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT 
AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC 
ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG 
CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC 
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG 
GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT 
TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA 
GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC 
CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC 
GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT 
GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC 
CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC 
TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT 
GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC 
CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG 
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC 
CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT 
CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC 
GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC 
TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT 
GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG 
TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA 
GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG 
AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC 
AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG 
TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA 
CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC 
GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG 
CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG 
GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG 
GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT 
AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG 
GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC 
TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC 
CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG 
TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG 
GCCGTGACAT CTGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG 
AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA 
GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC 
GGCAGCCCCT ACGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC 
AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC 
GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC 
ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG 
CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC 
AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT 
ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC 
TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIN2B ( NM_001192921.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179850.1 Bos taurus glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), mRNA.

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CloneID	OBa36350
Clone ID Related Accession (Same CDS sequence)	NM_001192921.1
Accession Version	NM_001192921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1420214400000
Organism	Bos taurus(Cattle)
Product	glutamate receptor ionotropic, NMDA 2B precursor
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03086648.1, AAFC03071459.1, AAFC02060016.1 and AAFC03054766.1. On Jul 17, 2010 this sequence version replaced XM_617989.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC 
CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC 
CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC 
ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT 
GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC 
ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC 
TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC 
ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG 
GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG 
GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC 
AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC 
TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT 
CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG 
TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC 
ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC 
TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC 
AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA 
AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA 
GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG 
CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG 
AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC 
ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC 
TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG 
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG 
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC 
GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC 
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC 
ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT 
AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC 
ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG 
CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC 
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG 
GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT 
TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA 
GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC 
CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC 
GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT 
GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC 
CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC 
TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT 
GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC 
CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG 
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC 
CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT 
CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC 
GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC 
TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT 
GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG 
TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA 
GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG 
AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC 
AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG 
TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA 
CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC 
GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG 
CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG 
GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG 
GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT 
AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG 
GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC 
TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC 
CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG 
TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG 
GCCGTGACAT CTGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG 
AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA 
GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC 
GGCAGCCCCT ATGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC 
AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC 
GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC 
ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG 
CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC 
AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT 
ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC 
TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179850.1
CDS	1..4494
Translation	MKPRAECCSPKFWLVLAVLAVSGSRARSQKSPPSIGIAVILVGTSDEVAIKDAHEKDDFHHLSVVPRVELVAMNETDPKSIITRICDLMSDRKIQGVVFADDTDQEAIAQILDFISAQTLTPILGIHGGSSMIMADKDESSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLDDGDSKIQNQLKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPSEFPTGLVSVSYDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLINVTFEGRNLSFSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETEEQEDDHLSIVTLEEAPFVIVESVDPLSGTCMRNTVPCQKRIISENKTDEEPGYIKKCCKGFCIDILKKISKSVKFTYDLYLVTNGKHGKKINGTWNGMIGEVVMKRAYMAVGSLTINEERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYAEMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAIQKDSGWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDIDNMAGVFYMLGAAMALSLITFICEHLFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMANLSGVNGSPQSALDFIRRESSVYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNVYQDHYHHHHRPHSIGSASSIDGLYDCDNPPFTTQPRALGKKPLDIGLPSSKHSQLGDLYGKFSFKSDRYGGHDDLIRSDVSDISTHTVTYGNIEGNAAKRRKQQYKDSLKKRPASAKSRREFDEIELAYRRRPPRSPDHKRYFRDKDGLRDFYLDQFRAKETSPHWEHVDLTDLYKERGEDFKRDSVGGGGPCTNRAHHLKHGTGDKHGGGVVGGVPAPWEKNLTNVDWEERPGGNFCRSCPSKLHNYSTTTAATAVVAGQNSGRPACIRCEACKKAGNLYDISEDNSLQELDQPAAPVAAVTSAASKYPQSPTNSKAQKKPRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRFLDGSPYAHMFEMPAGESAFSGSNDGKAAAPAAGHHHHGNPGGGGGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHGSKSYFFRQPTVAGAPKARPDFRALVSNKPVVSALHGAVPGRFQKDICIGNQSNPCVPNNKNPRAFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	NM_001192921.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192921.1

ORF Insert Sequence:

ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC 
CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC 
CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC 
ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT 
GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC 
ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC 
TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC 
ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG 
GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG 
GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC 
AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC 
TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT 
CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG 
TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC 
ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC 
TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC 
AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA 
AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA 
GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG 
CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG 
AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC 
ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC 
TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG 
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG 
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC 
GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC 
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC 
ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT 
AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC 
ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG 
CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC 
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG 
GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT 
TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA 
GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC 
CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC 
GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT 
GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC 
CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC 
TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT 
GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC 
CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG 
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC 
CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT 
CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC 
GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC 
TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT 
GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG 
TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA 
GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG 
AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC 
AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG 
TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA 
CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC 
GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG 
CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG 
GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG 
GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT 
AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG 
GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC 
TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC 
CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG 
TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG 
GCCGTGACAT CTGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG 
AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA 
GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC 
GGCAGCCCCT ATGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC 
AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC 
GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC 
ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG 
CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC 
AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT 
ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC 
TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIN2B ( XM_005206988.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207045.1 Bos taurus glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X1, mRNA.

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CloneID	OBa19686
Clone ID Related Accession (Same CDS sequence)	NM_001192921.2 , XM_005206988.3
Accession Version	XM_005206988.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	glutamate receptor ionotropic, NMDA 2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103935.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC 
CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC 
CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC 
ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT 
GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC 
ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC 
TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC 
ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG 
GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG 
GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC 
AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC 
TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT 
CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG 
TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC 
ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC 
TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC 
AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA 
AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA 
GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG 
CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG 
AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC 
ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC 
TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG 
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG 
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC 
GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC 
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC 
ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT 
AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC 
ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG 
CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC 
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG 
GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT 
TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA 
GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC 
CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC 
GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT 
GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC 
CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC 
TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT 
GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC 
CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG 
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC 
CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT 
CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC 
GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC 
TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT 
GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG 
TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA 
GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG 
AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC 
AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG 
TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA 
CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC 
GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG 
CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG 
GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG 
GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT 
AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG 
GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC 
TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC 
CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG 
TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG 
GCCGTGACAT CCGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG 
AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA 
GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC 
GGCAGCCCCT ATGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC 
AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC 
GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC 
ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG 
CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC 
AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT 
ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC 
TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207045.1
CDS	780..5273
Translation	MKPRAECCSPKFWLVLAVLAVSGSRARSQKSPPSIGIAVILVGTSDEVAIKDAHEKDDFHHLSVVPRVELVAMNETDPKSIITRICDLMSDRKIQGVVFADDTDQEAIAQILDFISAQTLTPILGIHGGSSMIMADKDESSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLDDGDSKIQNQLKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPSEFPTGLVSVSYDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLINVTFEGRNLSFSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETEEQEDDHLSIVTLEEAPFVIVESVDPLSGTCMRNTVPCQKRIISENKTDEEPGYIKKCCKGFCIDILKKISKSVKFTYDLYLVTNGKHGKKINGTWNGMIGEVVMKRAYMAVGSLTINEERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYAEMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAIQKDSGWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDIDNMAGVFYMLGAAMALSLITFICEHLFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMANLSGVNGSPQSALDFIRRESSVYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNVYQDHYHHHHRPHSIGSASSIDGLYDCDNPPFTTQPRALGKKPLDIGLPSSKHSQLGDLYGKFSFKSDRYGGHDDLIRSDVSDISTHTVTYGNIEGNAAKRRKQQYKDSLKKRPASAKSRREFDEIELAYRRRPPRSPDHKRYFRDKDGLRDFYLDQFRAKETSPHWEHVDLTDLYKERGEDFKRDSVGGGGPCTNRAHHLKHGTGDKHGGGVVGGVPAPWEKNLTNVDWEERPGGNFCRSCPSKLHNYSTTTAATAVVAGQNSGRPACIRCEACKKAGNLYDISEDNSLQELDQPAAPVAAVTSAASKYPQSPTNSKAQKKPRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRFLDGSPYAHMFEMPAGESAFSGSNDGKAAAPAAGHHHHGNPGGGGGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHGSKSYFFRQPTVAGAPKARPDFRALVSNKPVVSALHGAVPGRFQKDICIGNQSNPCVPNNKNPRAFNGSSNGHVYEKLSSIESDV

Target ORF information:

RefSeq Version	XM_005206988.3
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206988.3

ORF Insert Sequence:

ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC 
CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC 
CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC 
ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT 
GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC 
ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC 
TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC 
ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG 
GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG 
GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC 
AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC 
TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT 
CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG 
TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC 
ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC 
TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC 
AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA 
AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA 
GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG 
CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG 
AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC 
ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC 
TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG 
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG 
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC 
GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC 
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC 
ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT 
AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC 
ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG 
CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC 
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG 
GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT 
TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA 
GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC 
CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC 
GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT 
GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC 
CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC 
TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT 
GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC 
CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG 
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC 
CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT 
CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC 
GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC 
TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT 
GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG 
TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA 
GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG 
AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC 
AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG 
TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA 
CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC 
GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG 
CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG 
GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG 
GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT 
AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG 
GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC 
TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC 
CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG 
TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG 
GCCGTGACAT CCGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG 
AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA 
GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC 
GGCAGCCCCT ATGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC 
AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC 
GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC 
ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG 
CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC 
AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT 
ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC 
TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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GRIN2B cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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