MYO1F cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYO1F
Entrez Gene ID	532964
Full Name	myosin IF
General protein information	Preferred Names myosin IF Names unconventional myosin-If
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001192748.1	NP_001179677.1	unconventional myosin-If
XM_024994339.1	XP_024850107.1	unconventional myosin-If isoform X1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B12562g	XP_452090.1
Bos taurus (cattle)	MYO1F	NP_001179677.1
Gallus gallus (chicken)	MYO1F	NP_990585.1
Saccharomyces cerevisiae (baker's yeast)	MYO5	NP_013827.1
Homo sapiens (human)	MYO1F	NP_036467.2
Rattus norvegicus (Norway rat)	Myo1f	NP_001101546.1
Xenopus tropicalis (tropical clawed frog)	myo1f	XP_002938604.1
Schizosaccharomyces pombe (fission yeast)	myo1	NP_595402.1
Mus musculus (house mouse)	Myo1f	NP_444444.2
Eremothecium gossypii	AGOS_AEL306C	NP_984554.2
Magnaporthe oryzae (rice blast fungus)	MGG_00748	XP_003718252.1
Pan troglodytes (chimpanzee)	MYO1F	XP_512345.3
Saccharomyces cerevisiae (baker's yeast)	MYO3	NP_012793.2
Neurospora crassa	NCU02111	XP_964105.1
Canis lupus familiaris (dog)	MYO1F	XP_005633017.1
Danio rerio (zebrafish)	myo1f	NP_001243600.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MYO1F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYO1F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa36141	NM_001192748.1 Latest version!	Bos taurus myosin IF (MYO1F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa236097	XM_024994339.1 Latest version!	Bos taurus myosin IF (MYO1F), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYO1F ( NM_001192748.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179677.1 Bos taurus myosin IF (MYO1F), mRNA.

Price & Availability↑

CloneID	OBa36141
Clone ID Related Accession (Same CDS sequence)	NM_001192748.1
Accession Version	NM_001192748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527523200000
Organism	Bos taurus(cattle)
Product	unconventional myosin-If
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On Jul 17, 2010 this sequence version replaced XM_612193.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571261.1991.1, SRR5571263.48519.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGCAGCA AGGAGCGCTT CCACTGGCAG AGCCACAATG TGAAGCAGAG CGGCGTGGAC 
GACATGGTGC TTCTACCCCA GATCACTGAG GACGGCATCG TGGGCAACCT CCGCAAGCGC 
TTCATGGACG ACTACATCTT TACCTACATT GGCTCCGTGC TCATCTCCGT AAACCCCTTC 
AAGCAGATGC CCTACTTCAC AGACCGCGAG ATCGACCTCT ACCAGGGCGC GGCCCAATAT 
GAGAATCCCC CGCACATCTA TGCCCTCACC GACAATATGT ACCGGAACAT GCTTATAGAC 
TGTGAGAACC AATGTGTCAT TATCAGTGGA GAGAGTGGAG CTGGGAAGAC AGTGGCAGCA 
AAATATATCA TGGGCTACAT CTCCAAGGTG TCGGGCGGAG GCGAGAAGGT CCAGCATGTG 
AAAGATATCA TCCTTCAATC AAACCCACTG CTGGAAGCCT TTGGCAATGC CAAGACAGTG 
CGCAACAATA ATTCCAGTCG CTTTGGCAAG TACTTTGAGA TCCAGTTCAG CCGAGGGGGG 
GAGCCAGATG GGGGCAAGAT CTCCAACTTC CTGCTGGAGA AATCCCGCGT GGTCATGCAG 
AATGAAAATG AGAGGAATTT CCACATCTAC TATCAGCTGC TGGAAGGGGC CTCTCAGGAG 
CAGCGGCAGA ACCTAGGGCT CATGACTCCC GACTACTATT ACTACCTCAA CCAGTCAGAC 
ACCTACAAGG TGGACGGCAC CGATGACCGG AGCGACTTCA GTGAGACTCT GAGCGCCATG 
CAAGTCATCG GGATCCCGGC CAACGTCCAA GAGCTGGTCC TGCAGCTTGT GGCAGGCATC 
TTGCATTTGG GAAACATCAG CTTCTGTGAA GACGGGAATT ATGCCCGGGT GGAAAGCGCA 
GACCTCCTGG CCTTCCCCGC CTACCTCCTG GGCATTGACA GTGGGCGGCT GCAGGAGAAA 
CTGACCAGCC GCAAGATGGA CAGCCGCTGG GGCGGACGCA GTGAGTCCAT TGACGTGACC 
CTGAACGTGG AGCAGGCAGC CTACACTCGG GATGCTCTGG CCAAGGGACT CTACTCCCGT 
CTCTTTGACT TCCTCGTGGA GGCCATCAAC CGTGCCATGC AGAAGCCCCA AGAGGAGTAC 
AGTATCGGCG TGCTTGACAT CTACGGCTTT GAGATATTCC AGAAAAACGG CTTTGAGCAG 
TTCTGCATCA ACTTTGTCAA TGAGAAACTG CAGCAGATCT TTATTGAACT CACTCTGAAG 
GCTGAACAGG AGGAGTACGT ACAGGAGGGT ATCCGCTGGA CCCCCATCCA GTACTTCAAC 
AACAAAATCG TCTGTGACCT CATCGAAAAC AAGCTGAACC CCCCAGGCAT CATGAGCGTC 
CTGGACGACG TATGCGCCAC CATGCACGCC ACGGGCGGCG GCGCCGATCA GACGCTGCTG 
CAGAAGCTGC AGGCAGCCGT GGGCAACCAC GAGCACTTCA ACAGCTGGAG CGCGGGCTTC 
GTCATCCACC ACTACGCTGG CAAGGTCTCC TATGATGTCA ACGGCTTCTG CGAGAGGAAC 
CGTGATGTCC TCTTCTCTGA CCTCATAGAG TTGATGCAGA CCAGTGAGCA GGCCTTCCTC 
CGGATGCTCT TTCCCGAGAA GCTGGACGTG GAAAAGAAGG GCCGCCCCAG CACTGCCGGT 
TCCAAGATCA AGAAACAAGC CAATGACCTG GTGTCCACGC TGAAAAGATG CACTCCCCAC 
TACATCCGAT GCATCAAGCC CAATGAGACC AAGAGGCCCC GCGACTGGGA GGAGAACAGG 
GTCAAGCACC AGGTGGAGTA CCTGGGCCTG AAGGAGAACA TCAGGGTGCG CCGCGCGGGC 
TTTGCCTACC GCCGCCAGTT CGCCAAGTTC CTGCAGAGGT ACGCCATTCT GACCCCCGAG 
ACGTGGCCGT ATTGGCGCGG GGACGAGCGT CAGGGGGTCC AGCACTTGCT GCGGGCAGTC 
AACATGGAGC CGGACCAGTA CCAGATGGGG AGCACCAAGG TCTTCGTTAA GAATCCTGAG 
TCCCTCTTCC TACTAGAGGA GATGAGAGAG CGCAAGTTCG ATGGCTTTGC CCGCACCATT 
CAGAAGGCCT GGCGGCGCCA TGTGGCAGTC CGGAAGTATG AGGAGATGCG GGAGGAAGCT 
TCCAACATCC TGCTGAACAA GAAGGAGCGG AGACGCAACA GCATCAATAG GAACTTTGTT 
GGGGACTACC TGGGGCTGGA GGAGCGGCCG GAGCTGCGTC AGTTCCTAGG CAAGAGAGAG 
CGCGTGGACT TTGCTGACTC AGTCAACAAG TATGACCGAC GCTTCAAGCC CATCAAGAGG 
GACTTGATCC TGACACCCAA GTGTGTGTAT GTGATCGGGC GGGAGAAGGT GAAGAAGGGG 
CCTGAGAAGG GCCAGGTGCG TGAAGTCCTG AAGAAGAAGC TGGAGATCCA GGCGCTCCGG 
GGAGTTTCTC TCAGCACGCG GCAGGATGAC TTCTTCATCC TCCAAGAAGA CGCGGCCGAC 
AGTTTCTTGG AAAGCATCTT CAAGACTGAG TTCGTCAGCC TCCTGTGCAA GCGCTTCGAG 
GAGGCGGTGC GGAGGCCCCT GGCCCTCACC TTCAGCGACA CACTACAGTT CCGGGTGAAG 
AAGGAGGGCT GGGGCGGAGG TGGCACCCGA AGCGTCACTT TCTCGCGTGG TTCCGGCGAC 
GTGGCGGTGC TCAAGGCTAG CGGCAAGGCC CTCACAGTCA GCGTGGGTGA CGGACTGCCC 
AAAAGCTCCA AGCCTACACG GAAGGGGATG GCCCAGGGAA AATCTAGAAG GCCAGGCCAG 
ACTGCTACCC GGGCAGCTCC TGGGCCCCCC AGAGGCCTGG ACCGCAATGG GGTGCCTCCC 
TCTGCCAGAA GGGGCCCCTT GCCCATGGAG ATCACATCTG GAGGGGGCTC CCAGCGGCCA 
CCACGGGGCC CTCCGTCCTC AGCCTTGGGG GCCAGCAGAC GCCCACGGGC ACGGCCCCCC 
TCGGAGCACA ACACAGAATT CCTCAATGTG CCTGACCAGG GCGTGGCTGG CATGCAGAGG 
AAGCGCAGCG TGGGACAGCG ACCTGTGCCT GGTGTAGGCC GACCTAAGCC CCAGCCACGG 
ATCCATGGCC CCAGGTGCCG GGCACTGTAT CAGTATGTCG GCCAAGACGT GGATGAGCTG 
AGCTTCAATG TGAACGAGGT CATCGAGATC CTTATGGAAG ATTCGTCAGG TTGGTGGAAA 
GGCCGGCTGC ATGGCCAGGA GGGCCTCTTC CCCGGAAACT ATGTGGAGAA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179677.1
CDS	115..3411
Translation	MGSKERFHWQSHNVKQSGVDDMVLLPQITEDGIVGNLRKRFMDDYIFTYIGSVLISVNPFKQMPYFTDREIDLYQGAAQYENPPHIYALTDNMYRNMLIDCENQCVIISGESGAGKTVAAKYIMGYISKVSGGGEKVQHVKDIILQSNPLLEAFGNAKTVRNNNSSRFGKYFEIQFSRGGEPDGGKISNFLLEKSRVVMQNENERNFHIYYQLLEGASQEQRQNLGLMTPDYYYYLNQSDTYKVDGTDDRSDFSETLSAMQVIGIPANVQELVLQLVAGILHLGNISFCEDGNYARVESADLLAFPAYLLGIDSGRLQEKLTSRKMDSRWGGRSESIDVTLNVEQAAYTRDALAKGLYSRLFDFLVEAINRAMQKPQEEYSIGVLDIYGFEIFQKNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVQEGIRWTPIQYFNNKIVCDLIENKLNPPGIMSVLDDVCATMHATGGGADQTLLQKLQAAVGNHEHFNSWSAGFVIHHYAGKVSYDVNGFCERNRDVLFSDLIELMQTSEQAFLRMLFPEKLDVEKKGRPSTAGSKIKKQANDLVSTLKRCTPHYIRCIKPNETKRPRDWEENRVKHQVEYLGLKENIRVRRAGFAYRRQFAKFLQRYAILTPETWPYWRGDERQGVQHLLRAVNMEPDQYQMGSTKVFVKNPESLFLLEEMRERKFDGFARTIQKAWRRHVAVRKYEEMREEASNILLNKKERRRNSINRNFVGDYLGLEERPELRQFLGKRERVDFADSVNKYDRRFKPIKRDLILTPKCVYVIGREKVKKGPEKGQVREVLKKKLEIQALRGVSLSTRQDDFFILQEDAADSFLESIFKTEFVSLLCKRFEEAVRRPLALTFSDTLQFRVKKEGWGGGGTRSVTFSRGSGDVAVLKASGKALTVSVGDGLPKSSKPTRKGMAQGKSRRPGQTATRAAPGPPRGLDRNGVPPSARRGPLPMEITSGGGSQRPPRGPPSSALGASRRPRARPPSEHNTEFLNVPDQGVAGMQRKRSVGQRPVPGVGRPKPQPRIHGPRCRALYQYVGQDVDELSFNVNEVIEILMEDSSGWWKGRLHGQEGLFPGNYVEKI

Target ORF information:

RefSeq Version	NM_001192748.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin IF (MYO1F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192748.1

ORF Insert Sequence:

ATGGGCAGCA AGGAGCGCTT CCACTGGCAG AGCCACAATG TGAAGCAGAG CGGCGTGGAC 
GACATGGTGC TTCTACCCCA GATCACTGAG GACGGCATCG TGGGCAACCT CCGCAAGCGC 
TTCATGGACG ACTACATCTT TACCTACATT GGCTCCGTGC TCATCTCCGT AAACCCCTTC 
AAGCAGATGC CCTACTTCAC AGACCGCGAG ATCGACCTCT ACCAGGGCGC GGCCCAATAT 
GAGAATCCCC CGCACATCTA TGCCCTCACC GACAATATGT ACCGGAACAT GCTTATAGAC 
TGTGAGAACC AATGTGTCAT TATCAGTGGA GAGAGTGGAG CTGGGAAGAC AGTGGCAGCA 
AAATATATCA TGGGCTACAT CTCCAAGGTG TCGGGCGGAG GCGAGAAGGT CCAGCATGTG 
AAAGATATCA TCCTTCAATC AAACCCACTG CTGGAAGCCT TTGGCAATGC CAAGACAGTG 
CGCAACAATA ATTCCAGTCG CTTTGGCAAG TACTTTGAGA TCCAGTTCAG CCGAGGGGGG 
GAGCCAGATG GGGGCAAGAT CTCCAACTTC CTGCTGGAGA AATCCCGCGT GGTCATGCAG 
AATGAAAATG AGAGGAATTT CCACATCTAC TATCAGCTGC TGGAAGGGGC CTCTCAGGAG 
CAGCGGCAGA ACCTAGGGCT CATGACTCCC GACTACTATT ACTACCTCAA CCAGTCAGAC 
ACCTACAAGG TGGACGGCAC CGATGACCGG AGCGACTTCA GTGAGACTCT GAGCGCCATG 
CAAGTCATCG GGATCCCGGC CAACGTCCAA GAGCTGGTCC TGCAGCTTGT GGCAGGCATC 
TTGCATTTGG GAAACATCAG CTTCTGTGAA GACGGGAATT ATGCCCGGGT GGAAAGCGCA 
GACCTCCTGG CCTTCCCCGC CTACCTCCTG GGCATTGACA GTGGGCGGCT GCAGGAGAAA 
CTGACCAGCC GCAAGATGGA CAGCCGCTGG GGCGGACGCA GTGAGTCCAT TGACGTGACC 
CTGAACGTGG AGCAGGCAGC CTACACTCGG GATGCTCTGG CCAAGGGACT CTACTCCCGT 
CTCTTTGACT TCCTCGTGGA GGCCATCAAC CGTGCCATGC AGAAGCCCCA AGAGGAGTAC 
AGTATCGGCG TGCTTGACAT CTACGGCTTT GAGATATTCC AGAAAAACGG CTTTGAGCAG 
TTCTGCATCA ACTTTGTCAA TGAGAAACTG CAGCAGATCT TTATTGAACT CACTCTGAAG 
GCTGAACAGG AGGAGTACGT ACAGGAGGGT ATCCGCTGGA CCCCCATCCA GTACTTCAAC 
AACAAAATCG TCTGTGACCT CATCGAAAAC AAGCTGAACC CCCCAGGCAT CATGAGCGTC 
CTGGACGACG TATGCGCCAC CATGCACGCC ACGGGCGGCG GCGCCGATCA GACGCTGCTG 
CAGAAGCTGC AGGCAGCCGT GGGCAACCAC GAGCACTTCA ACAGCTGGAG CGCGGGCTTC 
GTCATCCACC ACTACGCTGG CAAGGTCTCC TATGATGTCA ACGGCTTCTG CGAGAGGAAC 
CGTGATGTCC TCTTCTCTGA CCTCATAGAG TTGATGCAGA CCAGTGAGCA GGCCTTCCTC 
CGGATGCTCT TTCCCGAGAA GCTGGACGTG GAAAAGAAGG GCCGCCCCAG CACTGCCGGT 
TCCAAGATCA AGAAACAAGC CAATGACCTG GTGTCCACGC TGAAAAGATG CACTCCCCAC 
TACATCCGAT GCATCAAGCC CAATGAGACC AAGAGGCCCC GCGACTGGGA GGAGAACAGG 
GTCAAGCACC AGGTGGAGTA CCTGGGCCTG AAGGAGAACA TCAGGGTGCG CCGCGCGGGC 
TTTGCCTACC GCCGCCAGTT CGCCAAGTTC CTGCAGAGGT ACGCCATTCT GACCCCCGAG 
ACGTGGCCGT ATTGGCGCGG GGACGAGCGT CAGGGGGTCC AGCACTTGCT GCGGGCAGTC 
AACATGGAGC CGGACCAGTA CCAGATGGGG AGCACCAAGG TCTTCGTTAA GAATCCTGAG 
TCCCTCTTCC TACTAGAGGA GATGAGAGAG CGCAAGTTCG ATGGCTTTGC CCGCACCATT 
CAGAAGGCCT GGCGGCGCCA TGTGGCAGTC CGGAAGTATG AGGAGATGCG GGAGGAAGCT 
TCCAACATCC TGCTGAACAA GAAGGAGCGG AGACGCAACA GCATCAATAG GAACTTTGTT 
GGGGACTACC TGGGGCTGGA GGAGCGGCCG GAGCTGCGTC AGTTCCTAGG CAAGAGAGAG 
CGCGTGGACT TTGCTGACTC AGTCAACAAG TATGACCGAC GCTTCAAGCC CATCAAGAGG 
GACTTGATCC TGACACCCAA GTGTGTGTAT GTGATCGGGC GGGAGAAGGT GAAGAAGGGG 
CCTGAGAAGG GCCAGGTGCG TGAAGTCCTG AAGAAGAAGC TGGAGATCCA GGCGCTCCGG 
GGAGTTTCTC TCAGCACGCG GCAGGATGAC TTCTTCATCC TCCAAGAAGA CGCGGCCGAC 
AGTTTCTTGG AAAGCATCTT CAAGACTGAG TTCGTCAGCC TCCTGTGCAA GCGCTTCGAG 
GAGGCGGTGC GGAGGCCCCT GGCCCTCACC TTCAGCGACA CACTACAGTT CCGGGTGAAG 
AAGGAGGGCT GGGGCGGAGG TGGCACCCGA AGCGTCACTT TCTCGCGTGG TTCCGGCGAC 
GTGGCGGTGC TCAAGGCTAG CGGCAAGGCC CTCACAGTCA GCGTGGGTGA CGGACTGCCC 
AAAAGCTCCA AGCCTACACG GAAGGGGATG GCCCAGGGAA AATCTAGAAG GCCAGGCCAG 
ACTGCTACCC GGGCAGCTCC TGGGCCCCCC AGAGGCCTGG ACCGCAATGG GGTGCCTCCC 
TCTGCCAGAA GGGGCCCCTT GCCCATGGAG ATCACATCTG GAGGGGGCTC CCAGCGGCCA 
CCACGGGGCC CTCCGTCCTC AGCCTTGGGG GCCAGCAGAC GCCCACGGGC ACGGCCCCCC 
TCGGAGCACA ACACAGAATT CCTCAATGTG CCTGACCAGG GCGTGGCTGG CATGCAGAGG 
AAGCGCAGCG TGGGACAGCG ACCTGTGCCT GGTGTAGGCC GACCTAAGCC CCAGCCACGG 
ATCCATGGCC CCAGGTGCCG GGCACTGTAT CAGTATGTCG GCCAAGACGT GGATGAGCTG 
AGCTTCAATG TGAACGAGGT CATCGAGATC CTTATGGAAG ATTCGTCAGG TTGGTGGAAA 
GGCCGGCTGC ATGGCCAGGA GGGCCTCTTC CCCGGAAACT ATGTGGAGAA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO1F ( XM_024994339.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850107.1 Bos taurus myosin IF (MYO1F), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa236097
Clone ID Related Accession (Same CDS sequence)	XM_024994339.1
Accession Version	XM_024994339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	unconventional myosin-If isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGC CCTTGGTTCT CCCTTCTTTG GCACCAGGGA AGCTGCAACC CCATGCCTCT 
ACTGGCAGCA AGGAGCGCTT CCACTGGCAG AGCCACAATG TGAAGCAGAG CGGCGTGGAC 
GACATGGTGC TTCTACCCCA GATCACTGAG GACGGCATCG TGGGCAACCT CCGCAAGCGC 
TTCATGGACG ACTACATCTT TACCTACATT GGCTCCGTGC TCATCTCCGT AAACCCCTTC 
AAGCAGATGC CCTACTTCAC AGACCGCGAG ATCGACCTCT ACCAGGGCGC GGCCCAATAT 
GAGAATCCCC CGCACATCTA TGCCCTCACC GACAATATGT ACCGGAACAT GCTTATAGAC 
TGTGAGAACC AATGTGTCAT TATCAGTGGA GAGAGTGGAG CTGGGAAGAC AGTGGCAGCA 
AAATATATCA TGGGCTACAT CTCCAAGGTG TCGGGCGGAG GCGAGAAGGT CCAGCATGTG 
AAAGATATCA TCCTTCAATC AAACCCACTG CTGGAAGCCT TTGGCAATGC CAAGACAGTG 
CGCAACAATA ATTCCAGTCG CTTTGGCAAG TACTTTGAGA TCCAGTTCAG CCGAGGGGGG 
GAGCCAGATG GGGGCAAGAT CTCCAACTTC CTGCTGGAGA AATCCCGCGT GGTCATGCAG 
AATGAAAATG AGAGGAATTT CCACATCTAC TATCAGCTGC TGGAAGGGGC CTCTCAGGAG 
CAGCGGCAGA ACCTAGGGCT CATGACTCCC GACTACTATT ACTACCTCAA CCAGTCAGAC 
ACCTACAAGG TGGACGGCAC CGATGACCGG AGCGACTTCA GTGAGACTCT GAGCGCCATG 
CAAGTCATCG GGATCCCGGC CAACGTCCAA GAGCTGGTCC TGCAGCTTGT GGCAGGCATC 
TTGCATTTGG GAAACATCAG CTTCTGTGAA GACGGGAATT ATGCCCGGGT GGAAAGCGCA 
GACCTCCTGG CCTTCCCCGC CTACCTCCTG GGCATTGACA GTGGGCGGCT GCAGGAGAAA 
CTGACCAGCC GCAAGATGGA CAGCCGCTGG GGCGGACGCA GTGAGTCCAT TGACGTGACC 
CTGAACGTGG AGCAGGCAGC CTACACTCGG GATGCTCTGG CCAAGGGACT CTACTCCCGT 
CTCTTTGACT TCCTCGTGGA GGCCATCAAC CGTGCCATGC AGAAGCCCCA AGAGGAGTAC 
AGTATCGGCG TGCTTGACAT CTACGGCTTT GAGATATTCC AGAAAAACGG CTTTGAGCAG 
TTCTGCATCA ACTTTGTCAA TGAGAAACTG CAGCAGATCT TTATTGAACT CACTCTGAAG 
GCTGAACAGG AGGAGTACGT ACAGGAGGGT ATCCGCTGGA CCCCCATCCA GTACTTCAAC 
AACAAAATCG TCTGTGACCT CATCGAAAAC AAGCTGAACC CCCCAGGCAT CATGAGCGTC 
CTGGACGACG TATGCGCCAC CATGCACGCC ACGGGCGGCG GCGCCGATCA GACGCTGCTG 
CAGAAGCTGC AGGCAGCCGT GGGCAACCAC GAGCACTTCA ACAGCTGGAG CGCGGGCTTC 
GTCATCCACC ACTACGCTGG CAAGGTCTCC TATGATGTCA ACGGCTTCTG CGAGAGGAAC 
CGTGATGTCC TCTTCTCTGA CCTCATAGAG TTGATGCAGA CCAGTGAGCA GGCCTTCCTC 
CGGATGCTCT TTCCCGAGAA GCTGGACGTG GAAAAGAAGG GCCGCCCCAG CACTGCCGGT 
TCCAAGATCA AGAAACAAGC CAATGACCTG GTGTCCACGC TGAAAAGATG CACTCCCCAC 
TACATCCGAT GCATCAAGCC CAATGAGACC AAGAGGCCCC GCGACTGGGA GGAGAACAGG 
GTCAAGCACC AGGTGGAGTA CCTGGGCCTG AAGGAGAACA TCAGGGTGCG CCGCGCGGGC 
TTTGCCTACC GCCGCCAGTT CGCCAAGTTC CTGCAGAGGT ACGCCATTCT GACCCCCGAG 
ACGTGGCCGT ATTGGCGCGG GGACGAGCGT CAGGGGGTCC AGCACTTGCT GCGGGCAGTC 
AACATGGAGC CGGACCAGTA CCAGATGGGG AGCACCAAGG TCTTCGTTAA GAATCCTGAG 
TCCCTCTTCC TACTAGAGGA GATGAGAGAG CGCAAGTTCG ATGGCTTTGC CCGCACCATT 
CAGAAGGCCT GGCGGCGCCA TGTGGCAGTC CGGAAGTATG AGGAGATGCG GGAGGAAGCT 
TCCAACATCC TGCTGAACAA GAAGGAGCGG AGACGCAACA GCATCAATAG GAACTTTGTT 
GGGGACTACC TGGGGCTGGA GGAGCGGCCG GAGCTGCGTC AGTTCCTAGG CAAGAGAGAG 
CGCGTGGACT TTGCTGACTC AGTCAACAAG TATGACCGAC GCTTCAAGCC CATCAAGAGG 
GACTTGATCC TGACACCCAA GTGTGTGTAT GTGATCGGGC GGGAGAAGGT GAAGAAGGGG 
CCTGAGAAGG GCCAGGTGCG TGAAGTCCTG AAGAAGAAGC TGGAGATCCA GGCGCTCCGG 
GGAGTTTCTC TCAGCACGCG GCAGGATGAC TTCTTCATCC TCCAAGAAGA CGCGGCCGAC 
AGTTTCTTGG AAAGCATCTT CAAGACTGAG TTCGTCAGCC TCCTGTGCAA GCGCTTCGAG 
GAGGCGGTGC GGAGGCCCCT GGCCCTCACC TTCAGCGACA CACTACAGTT CCGGGTGAAG 
AAGGAGGGCT GGGGCGGAGG TGGCACCCGA AGCGTCACTT TCTCGCGTGG TTCCGGCGAC 
GTGGCGGTGC TCAAGGCTAG CGGCAAGGCC CTCACAGTCA GCGTGGGTGA CGGACTGCCC 
AAAAGCTCCA AGCCTACACG GAAGGGGATG GCCCAGGGAA AATCTAGAAG GCCAGGCCAG 
ACTGCTACCC GGGCAGCTCC TGGGCCCCCC AGAGGCCTGG ACCGCAATGG GGTGCCTCCC 
TCTGCCAGAA GGGGCCCCTT GCCCATGGAG ATCACATCTG GAGGGGGCTC CCAGCGGCCA 
CCACGGGGCC CTCCGTCCTC AGCCTTGGGG GCCAGCAGAC GCCCACGGGC ACGGCCCCCC 
TCGGAGCACA ACACAGAATT CCTCAATGTG CCTGACCAGG GCGTGGCTGG CATGCAGAGG 
AAGCGCAGCG TGGGACAGCG ACCTGTGCCT GGTGTAGGCC GACCTAAGCC CCAGCCACGG 
ATCCATGGCC CCAGGTGCCG GGCACTGTAT CAGTATGTCG GCCAAGACGT GGATGAGCTG 
AGCTTCAATG TGAACGAGGT CATCGAGATC CTTATGGAAG ATTCGTCAGG TTGGTGGAAA 
GGCCGGCTGC ATGGCCAGGA GGGCCTCTTC CCCGGAAACT ATGTGGAGAA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850107.1
CDS	16..3372
Translation	MPQPLVLPSLAPGKLQPHASTGSKERFHWQSHNVKQSGVDDMVLLPQITEDGIVGNLRKRFMDDYIFTYIGSVLISVNPFKQMPYFTDREIDLYQGAAQYENPPHIYALTDNMYRNMLIDCENQCVIISGESGAGKTVAAKYIMGYISKVSGGGEKVQHVKDIILQSNPLLEAFGNAKTVRNNNSSRFGKYFEIQFSRGGEPDGGKISNFLLEKSRVVMQNENERNFHIYYQLLEGASQEQRQNLGLMTPDYYYYLNQSDTYKVDGTDDRSDFSETLSAMQVIGIPANVQELVLQLVAGILHLGNISFCEDGNYARVESADLLAFPAYLLGIDSGRLQEKLTSRKMDSRWGGRSESIDVTLNVEQAAYTRDALAKGLYSRLFDFLVEAINRAMQKPQEEYSIGVLDIYGFEIFQKNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVQEGIRWTPIQYFNNKIVCDLIENKLNPPGIMSVLDDVCATMHATGGGADQTLLQKLQAAVGNHEHFNSWSAGFVIHHYAGKVSYDVNGFCERNRDVLFSDLIELMQTSEQAFLRMLFPEKLDVEKKGRPSTAGSKIKKQANDLVSTLKRCTPHYIRCIKPNETKRPRDWEENRVKHQVEYLGLKENIRVRRAGFAYRRQFAKFLQRYAILTPETWPYWRGDERQGVQHLLRAVNMEPDQYQMGSTKVFVKNPESLFLLEEMRERKFDGFARTIQKAWRRHVAVRKYEEMREEASNILLNKKERRRNSINRNFVGDYLGLEERPELRQFLGKRERVDFADSVNKYDRRFKPIKRDLILTPKCVYVIGREKVKKGPEKGQVREVLKKKLEIQALRGVSLSTRQDDFFILQEDAADSFLESIFKTEFVSLLCKRFEEAVRRPLALTFSDTLQFRVKKEGWGGGGTRSVTFSRGSGDVAVLKASGKALTVSVGDGLPKSSKPTRKGMAQGKSRRPGQTATRAAPGPPRGLDRNGVPPSARRGPLPMEITSGGGSQRPPRGPPSSALGASRRPRARPPSEHNTEFLNVPDQGVAGMQRKRSVGQRPVPGVGRPKPQPRIHGPRCRALYQYVGQDVDELSFNVNEVIEILMEDSSGWWKGRLHGQEGLFPGNYVEKI

Target ORF information:

RefSeq Version	XM_024994339.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin IF (MYO1F), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994339.1

ORF Insert Sequence:

ATGCCCCAGC CCTTGGTTCT CCCTTCTTTG GCACCAGGGA AGCTGCAACC CCATGCCTCT 
ACTGGCAGCA AGGAGCGCTT CCACTGGCAG AGCCACAATG TGAAGCAGAG CGGCGTGGAC 
GACATGGTGC TTCTACCCCA GATCACTGAG GACGGCATCG TGGGCAACCT CCGCAAGCGC 
TTCATGGACG ACTACATCTT TACCTACATT GGCTCCGTGC TCATCTCCGT AAACCCCTTC 
AAGCAGATGC CCTACTTCAC AGACCGCGAG ATCGACCTCT ACCAGGGCGC GGCCCAATAT 
GAGAATCCCC CGCACATCTA TGCCCTCACC GACAATATGT ACCGGAACAT GCTTATAGAC 
TGTGAGAACC AATGTGTCAT TATCAGTGGA GAGAGTGGAG CTGGGAAGAC AGTGGCAGCA 
AAATATATCA TGGGCTACAT CTCCAAGGTG TCGGGCGGAG GCGAGAAGGT CCAGCATGTG 
AAAGATATCA TCCTTCAATC AAACCCACTG CTGGAAGCCT TTGGCAATGC CAAGACAGTG 
CGCAACAATA ATTCCAGTCG CTTTGGCAAG TACTTTGAGA TCCAGTTCAG CCGAGGGGGG 
GAGCCAGATG GGGGCAAGAT CTCCAACTTC CTGCTGGAGA AATCCCGCGT GGTCATGCAG 
AATGAAAATG AGAGGAATTT CCACATCTAC TATCAGCTGC TGGAAGGGGC CTCTCAGGAG 
CAGCGGCAGA ACCTAGGGCT CATGACTCCC GACTACTATT ACTACCTCAA CCAGTCAGAC 
ACCTACAAGG TGGACGGCAC CGATGACCGG AGCGACTTCA GTGAGACTCT GAGCGCCATG 
CAAGTCATCG GGATCCCGGC CAACGTCCAA GAGCTGGTCC TGCAGCTTGT GGCAGGCATC 
TTGCATTTGG GAAACATCAG CTTCTGTGAA GACGGGAATT ATGCCCGGGT GGAAAGCGCA 
GACCTCCTGG CCTTCCCCGC CTACCTCCTG GGCATTGACA GTGGGCGGCT GCAGGAGAAA 
CTGACCAGCC GCAAGATGGA CAGCCGCTGG GGCGGACGCA GTGAGTCCAT TGACGTGACC 
CTGAACGTGG AGCAGGCAGC CTACACTCGG GATGCTCTGG CCAAGGGACT CTACTCCCGT 
CTCTTTGACT TCCTCGTGGA GGCCATCAAC CGTGCCATGC AGAAGCCCCA AGAGGAGTAC 
AGTATCGGCG TGCTTGACAT CTACGGCTTT GAGATATTCC AGAAAAACGG CTTTGAGCAG 
TTCTGCATCA ACTTTGTCAA TGAGAAACTG CAGCAGATCT TTATTGAACT CACTCTGAAG 
GCTGAACAGG AGGAGTACGT ACAGGAGGGT ATCCGCTGGA CCCCCATCCA GTACTTCAAC 
AACAAAATCG TCTGTGACCT CATCGAAAAC AAGCTGAACC CCCCAGGCAT CATGAGCGTC 
CTGGACGACG TATGCGCCAC CATGCACGCC ACGGGCGGCG GCGCCGATCA GACGCTGCTG 
CAGAAGCTGC AGGCAGCCGT GGGCAACCAC GAGCACTTCA ACAGCTGGAG CGCGGGCTTC 
GTCATCCACC ACTACGCTGG CAAGGTCTCC TATGATGTCA ACGGCTTCTG CGAGAGGAAC 
CGTGATGTCC TCTTCTCTGA CCTCATAGAG TTGATGCAGA CCAGTGAGCA GGCCTTCCTC 
CGGATGCTCT TTCCCGAGAA GCTGGACGTG GAAAAGAAGG GCCGCCCCAG CACTGCCGGT 
TCCAAGATCA AGAAACAAGC CAATGACCTG GTGTCCACGC TGAAAAGATG CACTCCCCAC 
TACATCCGAT GCATCAAGCC CAATGAGACC AAGAGGCCCC GCGACTGGGA GGAGAACAGG 
GTCAAGCACC AGGTGGAGTA CCTGGGCCTG AAGGAGAACA TCAGGGTGCG CCGCGCGGGC 
TTTGCCTACC GCCGCCAGTT CGCCAAGTTC CTGCAGAGGT ACGCCATTCT GACCCCCGAG 
ACGTGGCCGT ATTGGCGCGG GGACGAGCGT CAGGGGGTCC AGCACTTGCT GCGGGCAGTC 
AACATGGAGC CGGACCAGTA CCAGATGGGG AGCACCAAGG TCTTCGTTAA GAATCCTGAG 
TCCCTCTTCC TACTAGAGGA GATGAGAGAG CGCAAGTTCG ATGGCTTTGC CCGCACCATT 
CAGAAGGCCT GGCGGCGCCA TGTGGCAGTC CGGAAGTATG AGGAGATGCG GGAGGAAGCT 
TCCAACATCC TGCTGAACAA GAAGGAGCGG AGACGCAACA GCATCAATAG GAACTTTGTT 
GGGGACTACC TGGGGCTGGA GGAGCGGCCG GAGCTGCGTC AGTTCCTAGG CAAGAGAGAG 
CGCGTGGACT TTGCTGACTC AGTCAACAAG TATGACCGAC GCTTCAAGCC CATCAAGAGG 
GACTTGATCC TGACACCCAA GTGTGTGTAT GTGATCGGGC GGGAGAAGGT GAAGAAGGGG 
CCTGAGAAGG GCCAGGTGCG TGAAGTCCTG AAGAAGAAGC TGGAGATCCA GGCGCTCCGG 
GGAGTTTCTC TCAGCACGCG GCAGGATGAC TTCTTCATCC TCCAAGAAGA CGCGGCCGAC 
AGTTTCTTGG AAAGCATCTT CAAGACTGAG TTCGTCAGCC TCCTGTGCAA GCGCTTCGAG 
GAGGCGGTGC GGAGGCCCCT GGCCCTCACC TTCAGCGACA CACTACAGTT CCGGGTGAAG 
AAGGAGGGCT GGGGCGGAGG TGGCACCCGA AGCGTCACTT TCTCGCGTGG TTCCGGCGAC 
GTGGCGGTGC TCAAGGCTAG CGGCAAGGCC CTCACAGTCA GCGTGGGTGA CGGACTGCCC 
AAAAGCTCCA AGCCTACACG GAAGGGGATG GCCCAGGGAA AATCTAGAAG GCCAGGCCAG 
ACTGCTACCC GGGCAGCTCC TGGGCCCCCC AGAGGCCTGG ACCGCAATGG GGTGCCTCCC 
TCTGCCAGAA GGGGCCCCTT GCCCATGGAG ATCACATCTG GAGGGGGCTC CCAGCGGCCA 
CCACGGGGCC CTCCGTCCTC AGCCTTGGGG GCCAGCAGAC GCCCACGGGC ACGGCCCCCC 
TCGGAGCACA ACACAGAATT CCTCAATGTG CCTGACCAGG GCGTGGCTGG CATGCAGAGG 
AAGCGCAGCG TGGGACAGCG ACCTGTGCCT GGTGTAGGCC GACCTAAGCC CCAGCCACGG 
ATCCATGGCC CCAGGTGCCG GGCACTGTAT CAGTATGTCG GCCAAGACGT GGATGAGCTG 
AGCTTCAATG TGAACGAGGT CATCGAGATC CTTATGGAAG ATTCGTCAGG TTGGTGGAAA 
GGCCGGCTGC ATGGCCAGGA GGGCCTCTTC CCCGGAAACT ATGTGGAGAA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MYO1F cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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