COL6A3 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010803869.2	XP_010802171.1	collagen alpha-3(VI) chain isoform X5
XM_005197963.3	XP_005198020.1	collagen alpha-3(VI) chain isoform X1
XM_005197964.3	XP_005198021.1	collagen alpha-3(VI) chain isoform X2
XM_010827157.2	XP_010825459.1	collagen alpha-3(VI) chain isoform X5
XM_010803870.2	XP_010802172.1	collagen alpha-3(VI) chain isoform X6
XM_005204978.3	XP_005205035.1	collagen alpha-3(VI) chain isoform X1
XM_003582038.4	XP_003582086.2	collagen alpha-3(VI) chain isoform X3
XM_005197965.3	XP_005198022.1	collagen alpha-3(VI) chain isoform X4
XM_024990265.1	XP_024846033.1	collagen alpha-3(VI) chain isoform X4
XM_005204979.3	XP_005205036.1	collagen alpha-3(VI) chain isoform X2
XM_002686571.5	XP_002686617.2	collagen alpha-3(VI) chain isoform X3
XM_024990263.1	XP_024846031.1	collagen alpha-3(VI) chain isoform X2
XM_024990266.1	XP_024846034.1	collagen alpha-3(VI) chain isoform X5
XM_024990264.1	XP_024846032.1	collagen alpha-3(VI) chain isoform X3
XM_024990267.1	XP_024846035.1	collagen alpha-3(VI) chain isoform X6
XM_024990262.1	XP_024846030.1	collagen alpha-3(VI) chain isoform X1
XM_010827158.2	XP_010825460.1	collagen alpha-3(VI) chain isoform X6
XM_005204980.3	XP_005205037.1	collagen alpha-3(VI) chain isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa51858	XM_010827157.2 Latest version!	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa51858	XM_024990266.1 Latest version!	Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa51858	XM_010803869.2 Latest version!	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa51859	XM_010803870.2 Latest version!	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa51859	XM_010827158.2 Latest version!	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa51859	XM_024990267.1 Latest version!	Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa03695	XM_005197963.3 Latest version!	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa03695	XM_005204978.3 Latest version!	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa03697	XM_005197964.3 Latest version!	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa03697	XM_005204979.3 Latest version!	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa03698	XM_002686571.5 Latest version!	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa03698	XM_003582038.4 Latest version!	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa03699	XM_005204980.3 Latest version!	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa03699	XM_005197965.3 Latest version!	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa03699	XM_024990265.1 Latest version!	Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa233420	XM_024990262.1 Latest version!	Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa233421	XM_024990263.1 Latest version!	Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa233422	XM_024990264.1 Latest version!	Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

COL6A3 ( XM_010803869.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802171.1 Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa51858
Clone ID Related Accession (Same CDS sequence)	XM_010803869.2 , XM_010827157.2 , XM_024990266.1
Accession Version	XM_010803869.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803869.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC 
CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT 
GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG 
TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC 
ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT 
CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG 
GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC 
GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC 
CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC 
CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT 
GGAACCACTG AAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC 
ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG 
AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC 
CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC 
AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT 
GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG 
GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG 
GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA 
TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG 
ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG 
GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG 
GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC 
GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG 
CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA 
GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA 
GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG 
AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG 
AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC 
CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC 
AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC 
AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC 
CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG 
ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC 
CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG 
CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG 
GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG 
GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG 
ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG 
TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC 
ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA 
GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG 
CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC 
GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT 
CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC 
AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT 
GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG 
GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT 
GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC 
GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG 
ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC 
TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC 
AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG 
GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC 
GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG 
GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG 
AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT 
GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG 
AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG 
ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG 
AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG 
ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT 
CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA 
ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT 
GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG 
GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC 
ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC 
ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA 
CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG 
GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG 
AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC 
TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC 
ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT 
GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC 
GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA 
ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA 
GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA 
GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA 
GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT 
GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT 
AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC 
GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC 
GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC 
CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT 
CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC 
AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA 
GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT 
CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC 
AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT 
TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC 
ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT 
CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC 
ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC 
CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA 
ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG 
AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG 
GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC 
CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT 
CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG 
GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC 
CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG 
TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG 
CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG 
CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC 
CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG 
CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA 
AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG 
AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC 
TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC 
GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC 
CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG 
TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC 
GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT 
AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC 
CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA 
TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA 
CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG 
GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG 
CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG 
ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC 
AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA 
GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG 
ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC 
CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG 
AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC 
CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC 
AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC 
ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG 
CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT 
GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG 
CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC 
GCGATGGGGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802171.1
CDS	340..8214
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_010803869.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803869.2

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC 
CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT 
GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG 
TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC 
ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT 
CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG 
GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC 
GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC 
CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC 
CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT 
GGAACCACTG AAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC 
ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG 
AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC 
CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC 
AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT 
GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG 
GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG 
GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA 
TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG 
ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG 
GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG 
GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC 
GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG 
CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA 
GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA 
GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG 
AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG 
AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC 
CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC 
AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC 
AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC 
CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG 
ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC 
CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG 
CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG 
GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG 
GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG 
ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG 
TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC 
ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA 
GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG 
CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC 
GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT 
CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC 
AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT 
GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG 
GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT 
GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC 
GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG 
ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC 
TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC 
AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG 
GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC 
GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG 
GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG 
AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT 
GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG 
AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG 
ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG 
AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG 
ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT 
CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA 
ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT 
GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG 
GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC 
ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC 
ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA 
CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG 
GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG 
AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC 
TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC 
ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT 
GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC 
GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA 
ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA 
GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA 
GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA 
GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT 
GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT 
AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC 
GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC 
GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC 
CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT 
CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC 
AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA 
GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT 
CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC 
AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT 
TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC 
ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT 
CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC 
ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC 
CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA 
ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG 
AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG 
GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC 
CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT 
CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG 
GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC 
CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG 
TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG 
CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG 
CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC 
CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG 
CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA 
AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG 
AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC 
TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC 
GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC 
CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG 
TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC 
GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT 
AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC 
CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA 
TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA 
CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG 
GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG 
CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG 
ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC 
AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA 
GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG 
ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC 
CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG 
AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC 
CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC 
AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC 
ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG 
CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT 
GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG 
CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC 
GCGATGGGGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_010827157.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825459.1 Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X5, mRNA.

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CloneID	OBa51858
Clone ID Related Accession (Same CDS sequence)	XM_010803869.2 , XM_010827157.2 , XM_024990266.1
Accession Version	XM_010827157.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644673.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC 
CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT 
GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG 
TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC 
ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT 
CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG 
GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC 
GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC 
CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC 
CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT 
GGAACCACTG AAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC 
ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG 
AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC 
CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC 
AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT 
GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG 
GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG 
GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA 
TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG 
ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG 
GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG 
GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC 
GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG 
CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA 
GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA 
GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG 
AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG 
AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC 
CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC 
AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC 
AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC 
CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG 
ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC 
CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG 
CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG 
GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG 
GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG 
ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG 
TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC 
ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA 
GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG 
CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC 
GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT 
CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC 
AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT 
GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG 
GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT 
GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC 
GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG 
ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC 
TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC 
AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG 
GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC 
GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG 
GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG 
AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT 
GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG 
AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG 
ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG 
AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG 
ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT 
CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA 
ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT 
GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG 
GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC 
ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC 
ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA 
CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG 
GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG 
AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC 
TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC 
ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT 
GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC 
GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA 
ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA 
GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA 
GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA 
GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT 
GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT 
AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC 
GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC 
GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC 
CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT 
CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC 
AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA 
GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT 
CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC 
AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT 
TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC 
ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT 
CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC 
ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC 
CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA 
ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG 
AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG 
GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC 
CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT 
CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG 
GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC 
CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG 
TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG 
CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG 
CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC 
CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG 
CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA 
AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG 
AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC 
TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC 
GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC 
CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG 
TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC 
GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT 
AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC 
CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA 
TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA 
CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG 
GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG 
CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG 
ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC 
AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA 
GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG 
ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC 
CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG 
AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC 
CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC 
AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC 
ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG 
CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT 
GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG 
CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC 
GCGATGGGGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825459.1
CDS	340..8214
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_010827157.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010827157.2

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC 
CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT 
GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG 
TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC 
ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT 
CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG 
GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC 
GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC 
CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC 
CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT 
GGAACCACTG AAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC 
ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG 
AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC 
CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC 
AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT 
GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG 
GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG 
GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA 
TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG 
ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG 
GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG 
GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC 
GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG 
CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA 
GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA 
GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG 
AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG 
AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC 
CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC 
AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC 
AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC 
CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG 
ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC 
CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG 
CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG 
GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG 
GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG 
ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG 
TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC 
ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA 
GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG 
CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC 
GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT 
CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC 
AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT 
GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG 
GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT 
GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC 
GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG 
ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC 
TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC 
AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG 
GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC 
GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG 
GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG 
AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT 
GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG 
AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG 
ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG 
AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG 
ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT 
CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA 
ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT 
GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG 
GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC 
ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC 
ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA 
CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG 
GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG 
AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC 
TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC 
ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT 
GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC 
GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA 
ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA 
GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA 
GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA 
GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT 
GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT 
AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC 
GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC 
GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC 
CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT 
CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC 
AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA 
GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT 
CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC 
AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT 
TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC 
ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT 
CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC 
ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC 
CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA 
ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG 
AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG 
GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC 
CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT 
CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG 
GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC 
CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG 
TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG 
CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG 
CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC 
CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG 
CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA 
AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG 
AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC 
TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC 
GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC 
CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG 
TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC 
GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT 
AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC 
CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA 
TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA 
CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG 
GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG 
CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG 
ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC 
AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA 
GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG 
ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC 
CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG 
AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC 
CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC 
AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC 
ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG 
CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT 
GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG 
CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC 
GCGATGGGGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_024990266.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846034.1 Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X5, mRNA.

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CloneID	OBa51858
Clone ID Related Accession (Same CDS sequence)	XM_010803869.2 , XM_010827157.2 , XM_024990266.1
Accession Version	XM_024990266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC 
CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT 
GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG 
TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC 
ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT 
CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG 
GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC 
GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC 
CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC 
CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT 
GGAACCACTG AAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC 
ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG 
AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC 
CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC 
AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT 
GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG 
GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG 
GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA 
TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG 
ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG 
GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG 
GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC 
GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG 
CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA 
GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA 
GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG 
AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG 
AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC 
CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC 
AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC 
AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC 
CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG 
ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC 
CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG 
CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG 
GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG 
GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG 
ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG 
TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC 
ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA 
GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG 
CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC 
GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT 
CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC 
AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT 
GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG 
GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT 
GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC 
GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG 
ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC 
TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC 
AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG 
GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC 
GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG 
GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG 
AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT 
GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG 
AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG 
ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG 
AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG 
ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT 
CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA 
ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT 
GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG 
GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC 
ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC 
ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA 
CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG 
GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG 
AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC 
TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC 
ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT 
GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC 
GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA 
ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA 
GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA 
GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA 
GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT 
GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT 
AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC 
GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC 
GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC 
CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT 
CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC 
AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA 
GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT 
CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC 
AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT 
TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC 
ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT 
CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC 
ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC 
CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA 
ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG 
AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG 
GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC 
CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT 
CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG 
GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC 
CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG 
TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG 
CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG 
CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC 
CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG 
CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA 
AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG 
AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC 
TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC 
GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC 
CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG 
TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC 
GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT 
AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC 
CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA 
TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA 
CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG 
GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG 
CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG 
ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC 
AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA 
GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG 
ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC 
CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG 
AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC 
CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC 
AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC 
ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG 
CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT 
GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG 
CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC 
GCGATGGGGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846034.1
CDS	276..8150
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_024990266.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990266.1

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC 
CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT 
GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG 
TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC 
ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT 
CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG 
GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC 
GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC 
CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC 
CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT 
GGAACCACTG AAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC 
ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG 
AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC 
CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC 
AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT 
GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG 
GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG 
GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA 
TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG 
ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG 
GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG 
GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC 
GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG 
CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA 
GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA 
GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG 
AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG 
AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC 
CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC 
AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC 
AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC 
CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG 
ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC 
CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG 
CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG 
GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG 
GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG 
ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG 
TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC 
ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA 
GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG 
CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC 
GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT 
CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC 
AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT 
GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG 
GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT 
GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC 
GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG 
ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC 
TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC 
AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG 
GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC 
GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG 
GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG 
AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT 
GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG 
AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG 
ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG 
AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG 
ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT 
CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA 
ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT 
GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG 
GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC 
ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC 
ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA 
CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG 
GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG 
AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC 
TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC 
ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT 
GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC 
GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA 
ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA 
GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA 
GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA 
GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT 
GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT 
AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC 
GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC 
GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC 
CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT 
CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC 
AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA 
GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT 
CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC 
AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT 
TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC 
ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT 
CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC 
ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC 
CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA 
ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG 
AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG 
GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC 
CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT 
CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG 
GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC 
CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG 
TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG 
CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG 
CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC 
CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG 
CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA 
AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG 
AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC 
TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC 
GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC 
CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG 
TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC 
GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT 
AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC 
CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA 
TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA 
CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG 
GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG 
CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG 
ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC 
AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA 
GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG 
ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC 
CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG 
AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC 
CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC 
AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC 
ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG 
CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT 
GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG 
CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC 
GCGATGGGGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_010803870.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802172.1 Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X6, mRNA.

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CloneID	OBa51859
Clone ID Related Accession (Same CDS sequence)	XM_010803870.2 , XM_024990267.1 , XM_010827158.2
Accession Version	XM_010803870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAG AGCAAGCGAG ACATCCTGTT CCTTTTCGAC 
GGCTCAGCCA ACCTCATGGG CCAGTTCACT GCCGCCCGTG ACTTTCTCTA CAAGGTCATC 
GACGAGCTGG ATGTGAAACC TGAGGGGACC CGGGTTGCAG TGGCTCAGTT CAGTGATGAT 
GTCAAGGTGG AGTCCCGTTT TGACCAGCAC CAGAATAAGC CTGAGATCCT GAATCTCGTG 
AAGAGGATGA AGCTCAAGAC AGGCAAAGCC CTCAACCTGG GCTACGCCTT GGATTACGCA 
CAGAGGTACA TCTTTGTGAA GTCCGCTGGA AGCCGGATTG AGGATGGAGT GCTTCAGTTC 
CTGGTGCTGC TGGTGGCGGG AAAGTCATCG GACCGGGTGG ATACACCCGC ACTCAACCTG 
AAGCAGAGCG GGGTGGTGCC CTTCATCCTC CAGGCGAAAA ACGCGGACCC TGCTGAGTTG 
GAGCTGATCG TACCATCCCC AGCCTTTATT TTGGTTGCGG AGTCCCTTCC TAAGATTGGA 
GACCTGCAAC CACAGATTGT GAACCTCTTA AAATCAGTGC AAAATGGGGC ACCGGCACCA 
GTTTCAGTTG AAAAGGATGT GGTGTTCCTG ATTGATGGCT CCGAGGGTGT CAGGAGCGGC 
TTCCCACTGC TGAAGGAGTT CGTCCAGAGA GTGGTGGAGA GCCTGGACGT GGGACCAGAC 
CGGGTGCGTG TGGCCGTGGT GCAGTACAGC GACCGGACCA GACCTGAGTT CTACCTGAAT 
TCCTACATGG ACCAGCAGAG TGTGGTCGGA GCCATCCGTG GGCTGACCCT GCTGGGCGGG 
CCGGCCCCCA ACACAGGGGC TGCCCTGGAG TTCGTCCTGA GGAACATCCT GGTGGGCTCT 
GCTGGGAGCC GCATAGCAGA AGGTGTCCCC CAGCTCCTGA TTGTCCTCAC GGCCGACAGG 
TCTGGGGATG ACGTGAGGGG CCCCTCTGTG GTCCTGAGGA GGGGAGGGGC CGTGCCCATC 
GGCATCGGCA TTGGGAACGC TGACATCACC GAGATGCAGA CCCTCTCCTT CGTCCCGGAC 
TTCGCCGTGG TCATCCCCAC CTTCCGGCAG CTGGGGACCA TCCAACAGGT CATCTCGGAG 
AGAGTGACCC AGCTCAGCCG CGAGGAACTG AGCAGGCTGC AGGCGTCTGT CACCCCCTTG 
ACGACTCCGG TGGTCAGCAG CAAGAGGGAC GTGGTCTTTC TCATCGATGG GTCCCAGAGT 
GCTGGCCCCG AGTTCCAGTA CATCCGAACG CTCATCGAGA GGCTGGTGGA CTACCTGGAT 
GTGGGCTTCG ACACGACCCG GGTGGCGGTC ATCCAGTTCA GCGATGACCC CAGGGTGGAG 
TTCCTGTTGA ACGTCCACTC CAGCAAGGAC GAGGTGCAGA ACGCCGTGCG GCGGCTGCGG 
CCCAAAGGTG GGAGGCAGGT CAACGTTGGG GGTGCCCTGG AGTACGTGGC CAGGAACATC 
TTCAAGAGGC CCCTGGGGAG CCGGATTGAG GAGGGTGTCC CTCAGTTCCT GGTCCTCATT 
TCCTCCGGGA AGTCGGATGA TGAGGTGGAG GACTCGGCCA TAGAGCTCAA GCAGTTTGGC 
GTGGCGCCAC TCACGATCGC CAGGAACGTG GACCAGGAAG AGCTGGTGAA GATCTCGCTG 
AGCCCCGAGT ACGTGTTCTC AGTGAACACC TTCCGGGAGC TCCCCAGCCT GGAGCAGAAA 
CTGCTGACAC CGCTCACCAC CCTGACTGCC GGGCAGATAC AGCAGCTCCT GGCCAGCACG 
CGCTACCCGC CTCCAGCTGT GGAGAGCGAT GCTGCGGACA TTGTCTTCCT CATCGACAGC 
TCTGATGGCG TGAAGCCGGA TGGCATCGCA CACATCCGGG ACTTTGTTAT CAGGATCGTG 
CGGAGACTCA ACGTCGGTCC CAACAAAGTG AGAATTGGGG TCCTGCAGTT CAGCAACGAC 
GTCTTCCCAG AATTTCAACT AAAGACCTAT AAATCCCAGG CCAGCGTGCT GGATGCCATA 
CGTCGCCTGA GGTTCAAAGG GGGGTCTCCT CTCAACACTG GCAAGGCTCT GGAATTCGTG 
GCGAGGAATT ACTTTGTCAA GTCTGCCGGG AGCCGGATAG AAGATGGGGT GCCCCAACAC 
CTGGTTCTAT TTCTGGGTGG AAAATCCCAG GATGATATTT CCAGGTATTC CCAGGTGATA 
AAATCTGCAG GAATTGCGAG CTTAGGGGTT GGAGACCGAA ATATTGACAG AACAGAGCTG 
CAGACCATCA CCAGCGATCC CAGGCTTGTC TTCACGGTGC GGGAGTTCAG AGACCTCCCC 
AGCATAGAAG AAAGGATGGT GAATTCCTTT GGGTCCTCCG GGGTCACCCC TGCACCTCCG 
GGAGTGGACA CACCCTCTCC TTCAAGGCCA GAGAAGAAGA AAGCCGACAT TGTGTTCCTG 
CTGGATGGTT CCATCAATTT CCGGAGAGAC AGTTTCCAGG AAGTGCTCCG TTTTGTGTCA 
GAAATTGTGG ACACGGTTTA TGAAGGGGGC GACTCCATCC AGGTGGGGCT GGTCCAATAC 
AACTCTGACC CCACCGACGA GTTCTTCCTG AAGGACTTCC CCACCAAGCA GCAGATTATC 
GACGCCATAA ACAAGGTGGT CTACAAAGGG GGGAGGCACG CGAACACCAA GGTGGGCCTG 
GAGCACCTGC GGCGGAACCA CTTCGTGCCC GAGGCGGGCA GCCGCCTGGA CCAGAGGGTC 
CCGCAGATCG CCTTTGTGAT CACGGGGGGC AAGTCGGTGG AGGACGCTCA GGAGGCAAGC 
ATGGCGCTCA CGCAGAGAGG CGTCAAGGTG TTTGCGGTGG GCGTAAGGAA CATCGACTCG 
GAGGAGGTTG GGAAGATAGC GTCCAACAGC GCCACGGCCT TCCGGGTGGG GAACGTCCAG 
GAGCTGTCGG AACTGAGCGA GCAGGTTTTG GAAACTCTGC ACGATGCAAT GCATGAGACC 
TTATGTCCAG GCGTGACTGA CGTTTCCAAA GCCTGTAACT TGGATGTGAT TCTGGGCTTC 
GATGGTTCAG GAGATCAGAA TGTCTTTGTG GCTCAGAAGG GCCTCGAGCC CAAGGTGGAC 
ACCATCCTGA GGAGAATCAG TCAGATGCAG AAGATCAGCT GCAGCGGCAG CCAGCTGCCC 
ACCGTGCGGG TGTCTGTGGT GGCCCTCACG CCCTCGGGCC CCGTTGAGGC CTTTGACTTT 
GCTGAGTACC AGCCGGAGCT GTTTGAGAAG TTCCAGAACA TGCGTGCCCA GCACCCCTAC 
GTCCTCACTG CGGACACACT GAAGCTCTAC CAGAACAAGT TCCGGCAGGC CTCGATGGAC 
AACGTGAAGG TGGTCATTCA TTTTACCGAT GGAGTGGATG GGGATCTGGC TGATTTACAA 
AGAGCATCAG AGGAACTCCG ACAAGAAGGT GTCCGAGCTC TGATCTTGGT GGGCCTCGAA 
CGCGTCGCCA ACTTGGAGCA GCTCATGCAG CTGGAGTTCG GGCGAGGGTT CACGTACAAC 
CGGCCCCTGA GGCTGAACTT GCTGGACCTG GACTACGAAC TAGCCGAGCA GCTTGACAAC 
ATTGCTGAGA AAGCCTGCTG TGGGATTCCC TGCAAATGCT CTGGACAAAG AGGAGACCGA 
GGGCCCATAG GCAGCATCGG GCCAAAGGGC ATTCCTGGAG AAGATGGCTA CCGAGGTTAT 
CCTGGTGACG AAGGTGGACC TGGTGAACGC GGTCCTCCTG GCGTGAACGG CACTCAAGGT 
TTCCAGGGCT GCCCCGGGCA GAGAGGAGTC AAGGGCTCTC GTGGATTCCC GGGAGAGAAG 
GGTGAATTAG GAGAAATTGG ACTGGATGGT CTTGATGGTG AAGATGGCGA CAAAGGGCTG 
CCCGGTACTT CTGGAGAGAA AGGGAGTCCA GGAAGGAGGG GCGACAAAGG ACCCAAAGGC 
GACAAAGGGG AGAGAGGCGA TGTCGGGATC AGAGGCGACC CGGGCAATTC AGGACAGGAC 
AGCCAGCAGA GAGGAGCCAA AGGAGAAACC GGAGACATCG GCCCCGTGGG TCTCCCTGGG 
AGAGACGGGG TGTCTGGAGG GCCTGGAGAA CCCGGGAAGA GCGGTGGCTT TGGCCGAAGG 
GGACCAGCGG GAGCTAAGGG CAACCAGGGC GGCCCCGGCC AGCAGGGCCC TGTAGGAGAG 
CAGGGGACCA GAGGCGCACA GGGTCCGCCT GGTCCCACCG GTCCTCCAGG CCTGATTGGT 
GAACAGGGCA TTCCCGGACC TCGGGGAGGC GGGGGAACCG CAGGTGCTCC TGGAGAACGC 
GGCAGAACCG GGCCCCTGGG AAGAAAGGGT GAGCCCGGAG ATCCAGGACC TAAGGGGAGC 
ATCGGGAACC GGGGTCCTCG TGGGGAGACG GGAGATGATG GGCGAGATGG AGTTGGCAGC 
GAAGGTCGCA TAGGCAAGAA GGGAGAAAGA GGATTCCCTG GATACCCAGG TCCAAAGGGC 
AATCCTGGTG AGCCAGGGAC AGACGGACCA CCGGGACCCA AAGGCATCAG AGGCCGAAGG 
GGAAACTCGG GACCTCCAGG GATAGCTGGA CAGAAGGGAG ACCCCGGTTA CCCAGGGCCA 
TCTGGTTACA AAGGCAGCAG AGGCGACTCA ATGGACCAAT GTGCGCTGGT CCAGAGCATC 
AAAGATAAAT GCCCTTGCTG TTACGGGCCC CTGGAGTGCC CCGTCTTCCC GACGGAGCTG 
GCTTTCGCCT TGGACACCTC AGAGGGGGTC ACCCAGGACA GGTTCAGCCA GATGAGGGAG 
GTGGTCCTCA AGATTCTGGA CGACCTGACC ATCGCCGAGA GCAACTGCCC GAGGGGGGCC 
CGCGTGGCCG TGGTCACTTA CAACAACGAG GTTACCACGG AGATCCGCTT TGCCGACTCC 
AAGAAGAAGT CCGTCCTCCT GGACAAGATC AAGAACCTGC AGGTGTCGCT GACCTCCAAG 
CAGCAGAGCC TGGAAACCGC CATGTCCTTT GTGGCCAGAA ACACGTTTAA GCGTGTGCGG 
AGCGGGTTCC TGATGAGGAA GGTGGCGGTG TTCTTCAGCA ACAAGCCCAC GAGGGCGACC 
CCGCAGCTCC GGGAGGCTGT GCTCAAGCTC TCAGATGCTG GGATCACACC CTTGTTCCTC 
ACAAGCCAGG AGGACCGGCA GCTGATCAAT GCCCTACAGA TCAATAACAC AGCGGTGGGA 
CATGCCCTTG TCCTTCCTGC TGGCGGAGAC CTCACAGACT TCTTGAAGAA TGTCCTGACG 
TGTCACGTCT GCTTGGACAT CTGCAACATT GACCCATCCT GTGGATTCGG CAGCTGGAGG 
CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATCGACAT GGCTTTCATC 
TTAGATAGCT CCGAGTCCAC CACCCCGTTC CAGTTCAACG AGATGAGGAA GTACATCGAG 
TACCTGGTCC GACAGCTGGA TGTGAGCCCA GACCCCAAGG CCTCCCAGCA CTTTGCCCGC 
GTGGCGGTGG TGCAGCATGC GCCCTACGAG GCGGTGGACA ACGCCAGTGT GCCGCCTGTG 
AAGGTGGAGT TCTCCCTGAC CGACTACGGC TCCAAAGAGA AGCTGCTGGC CTTCCTTGGC 
AGCAGGATGA CCCAGCTGCA GGGGACCAGG GCTTTGGGCC GCGCCATCGA CTACACCATT 
GAGAACATCT TCGAAAGTGC TCCCAACCCC CGGGACCTGA AGATCGTGGT GCTGATGCTG 
ACGGGCGAGG TGCAGAAGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT ACAGGCCAAG 
TGCAAAGGCT ACTTCTTCGT GATCCTGGGC ATTGGCAGGA AGGTGAATGT CAAGGAGCTG 
TACAGCTCTG CCAGCGAGCC CAACGACGTC TTCTTCAAGC TAGTGGACAA GTCGACCGAG 
CTCAACGAGG AGCCCCTAAT GCGCTTCGGG AGGCTGCTGC CATCCTTCGT CAGCAGTAAA 
GATGCTTTCT ACCTGTCCCC AGATATCAGG AAACAGTGTG ATTGGTTCCA AGCAGACCAA 
CCGGCAAAGA ATCTCGTGCA GTTTGGTTAC AAACAAATAA ATGTTCCTAA TAACGTTACT 
TCAAGTCCTA CATCTAAACT GGTGACCACA ACGAAGCCGG TGATCACCAC CACCAAACCG 
ATCACAGTGG TGAACCTGCC AGCCTCAAAG CCAGCTGCCA CGAGACCTGT GGATGAGAGA 
CCCGCAGCTG GGAGATCCGT GGCCACCAAG ATGGACAGTG CCAGGCCCGT GGCCACCAAG 
ACCGAGGCCA CCAAACCCGT GGCCACCAAG ACCGAGGCCA CCAAGACTGA AGCTGCCAAA 
CCTGTGGTCA CCAAGGCTGA GGCTGCAAAA CCCACGGCCA CCAAGACTGA AGCCACTAAG 
ACCGAGGCTG CAAAGCTCAT GGCCACCAAG ACCGAGGCCA CCAAACCTGT GGCTGCCAGG 
CCCGAGGCGG CAAAGACAGC CACAGCCAGA CCTGCAGCAG CCGCGAAGCC TGCAGCAGCC 
AAACCTGCCC CTGCCAGACC CCCCACTGCT GCCAGATCCA TGGCCACGAG GTCCGAGGCC 
CCCAGGCCCC AGGCAGTCAA ACTGGCTGCC AGCAGGCCCG GCGCTGCTAA GCCCGTGGTG 
AAGGCACCTC GCGAGATCCA TGTGTCTGAG GTCACAGAGA ACAGCGCCAA GCTGCACTGG 
GAGAGGCCCG AGCCCCCCAG CCCTTACTTA TACAACCTCA CCGTCACCTC GGCCCACGAT 
CAGGCCGTGG TTCTGAAGCA GAACCTCACT GTCACGGACC GCGTCATCGG GGGCCTGCTC 
CCCGGGCAGA CTTACCACGT TACGGTGACC TGCTCCCTGA GGTCTCAGGT CAGAGCCATC 
TACCAAGGCA GCTTCAGCAC GAAGAAAATT CAGCCTCCAC CTCCACAAAC AGAGCGATCA 
GCCTCTAGTG CAACCATCAA CCTAGTGGTG AGCGCAGACC GGTTGGCTGG GAGTAAAGCA 
GATATATGTA AGTTGCCAAA GGACGGCGGA ACTTGCAGAG AGTTTGTATT AAAGTGGTAC 
TATGATTCCG TAACTGAAAA CTGCGCCCGA TTCTGGTATG GAGGCTGCGG TGGAAACGAA 
AACAGATTTA ATTCGCAGGA TGAATGTGAA AAGGTTTGCC CTCCTGTGCC CATCAAGCAG 
CCCGGAGTTA TCGCCGCGAT GGGGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802172.1
CDS	338..7627
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_010803870.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803870.2

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAG AGCAAGCGAG ACATCCTGTT CCTTTTCGAC 
GGCTCAGCCA ACCTCATGGG CCAGTTCACT GCCGCCCGTG ACTTTCTCTA CAAGGTCATC 
GACGAGCTGG ATGTGAAACC TGAGGGGACC CGGGTTGCAG TGGCTCAGTT CAGTGATGAT 
GTCAAGGTGG AGTCCCGTTT TGACCAGCAC CAGAATAAGC CTGAGATCCT GAATCTCGTG 
AAGAGGATGA AGCTCAAGAC AGGCAAAGCC CTCAACCTGG GCTACGCCTT GGATTACGCA 
CAGAGGTACA TCTTTGTGAA GTCCGCTGGA AGCCGGATTG AGGATGGAGT GCTTCAGTTC 
CTGGTGCTGC TGGTGGCGGG AAAGTCATCG GACCGGGTGG ATACACCCGC ACTCAACCTG 
AAGCAGAGCG GGGTGGTGCC CTTCATCCTC CAGGCGAAAA ACGCGGACCC TGCTGAGTTG 
GAGCTGATCG TACCATCCCC AGCCTTTATT TTGGTTGCGG AGTCCCTTCC TAAGATTGGA 
GACCTGCAAC CACAGATTGT GAACCTCTTA AAATCAGTGC AAAATGGGGC ACCGGCACCA 
GTTTCAGTTG AAAAGGATGT GGTGTTCCTG ATTGATGGCT CCGAGGGTGT CAGGAGCGGC 
TTCCCACTGC TGAAGGAGTT CGTCCAGAGA GTGGTGGAGA GCCTGGACGT GGGACCAGAC 
CGGGTGCGTG TGGCCGTGGT GCAGTACAGC GACCGGACCA GACCTGAGTT CTACCTGAAT 
TCCTACATGG ACCAGCAGAG TGTGGTCGGA GCCATCCGTG GGCTGACCCT GCTGGGCGGG 
CCGGCCCCCA ACACAGGGGC TGCCCTGGAG TTCGTCCTGA GGAACATCCT GGTGGGCTCT 
GCTGGGAGCC GCATAGCAGA AGGTGTCCCC CAGCTCCTGA TTGTCCTCAC GGCCGACAGG 
TCTGGGGATG ACGTGAGGGG CCCCTCTGTG GTCCTGAGGA GGGGAGGGGC CGTGCCCATC 
GGCATCGGCA TTGGGAACGC TGACATCACC GAGATGCAGA CCCTCTCCTT CGTCCCGGAC 
TTCGCCGTGG TCATCCCCAC CTTCCGGCAG CTGGGGACCA TCCAACAGGT CATCTCGGAG 
AGAGTGACCC AGCTCAGCCG CGAGGAACTG AGCAGGCTGC AGGCGTCTGT CACCCCCTTG 
ACGACTCCGG TGGTCAGCAG CAAGAGGGAC GTGGTCTTTC TCATCGATGG GTCCCAGAGT 
GCTGGCCCCG AGTTCCAGTA CATCCGAACG CTCATCGAGA GGCTGGTGGA CTACCTGGAT 
GTGGGCTTCG ACACGACCCG GGTGGCGGTC ATCCAGTTCA GCGATGACCC CAGGGTGGAG 
TTCCTGTTGA ACGTCCACTC CAGCAAGGAC GAGGTGCAGA ACGCCGTGCG GCGGCTGCGG 
CCCAAAGGTG GGAGGCAGGT CAACGTTGGG GGTGCCCTGG AGTACGTGGC CAGGAACATC 
TTCAAGAGGC CCCTGGGGAG CCGGATTGAG GAGGGTGTCC CTCAGTTCCT GGTCCTCATT 
TCCTCCGGGA AGTCGGATGA TGAGGTGGAG GACTCGGCCA TAGAGCTCAA GCAGTTTGGC 
GTGGCGCCAC TCACGATCGC CAGGAACGTG GACCAGGAAG AGCTGGTGAA GATCTCGCTG 
AGCCCCGAGT ACGTGTTCTC AGTGAACACC TTCCGGGAGC TCCCCAGCCT GGAGCAGAAA 
CTGCTGACAC CGCTCACCAC CCTGACTGCC GGGCAGATAC AGCAGCTCCT GGCCAGCACG 
CGCTACCCGC CTCCAGCTGT GGAGAGCGAT GCTGCGGACA TTGTCTTCCT CATCGACAGC 
TCTGATGGCG TGAAGCCGGA TGGCATCGCA CACATCCGGG ACTTTGTTAT CAGGATCGTG 
CGGAGACTCA ACGTCGGTCC CAACAAAGTG AGAATTGGGG TCCTGCAGTT CAGCAACGAC 
GTCTTCCCAG AATTTCAACT AAAGACCTAT AAATCCCAGG CCAGCGTGCT GGATGCCATA 
CGTCGCCTGA GGTTCAAAGG GGGGTCTCCT CTCAACACTG GCAAGGCTCT GGAATTCGTG 
GCGAGGAATT ACTTTGTCAA GTCTGCCGGG AGCCGGATAG AAGATGGGGT GCCCCAACAC 
CTGGTTCTAT TTCTGGGTGG AAAATCCCAG GATGATATTT CCAGGTATTC CCAGGTGATA 
AAATCTGCAG GAATTGCGAG CTTAGGGGTT GGAGACCGAA ATATTGACAG AACAGAGCTG 
CAGACCATCA CCAGCGATCC CAGGCTTGTC TTCACGGTGC GGGAGTTCAG AGACCTCCCC 
AGCATAGAAG AAAGGATGGT GAATTCCTTT GGGTCCTCCG GGGTCACCCC TGCACCTCCG 
GGAGTGGACA CACCCTCTCC TTCAAGGCCA GAGAAGAAGA AAGCCGACAT TGTGTTCCTG 
CTGGATGGTT CCATCAATTT CCGGAGAGAC AGTTTCCAGG AAGTGCTCCG TTTTGTGTCA 
GAAATTGTGG ACACGGTTTA TGAAGGGGGC GACTCCATCC AGGTGGGGCT GGTCCAATAC 
AACTCTGACC CCACCGACGA GTTCTTCCTG AAGGACTTCC CCACCAAGCA GCAGATTATC 
GACGCCATAA ACAAGGTGGT CTACAAAGGG GGGAGGCACG CGAACACCAA GGTGGGCCTG 
GAGCACCTGC GGCGGAACCA CTTCGTGCCC GAGGCGGGCA GCCGCCTGGA CCAGAGGGTC 
CCGCAGATCG CCTTTGTGAT CACGGGGGGC AAGTCGGTGG AGGACGCTCA GGAGGCAAGC 
ATGGCGCTCA CGCAGAGAGG CGTCAAGGTG TTTGCGGTGG GCGTAAGGAA CATCGACTCG 
GAGGAGGTTG GGAAGATAGC GTCCAACAGC GCCACGGCCT TCCGGGTGGG GAACGTCCAG 
GAGCTGTCGG AACTGAGCGA GCAGGTTTTG GAAACTCTGC ACGATGCAAT GCATGAGACC 
TTATGTCCAG GCGTGACTGA CGTTTCCAAA GCCTGTAACT TGGATGTGAT TCTGGGCTTC 
GATGGTTCAG GAGATCAGAA TGTCTTTGTG GCTCAGAAGG GCCTCGAGCC CAAGGTGGAC 
ACCATCCTGA GGAGAATCAG TCAGATGCAG AAGATCAGCT GCAGCGGCAG CCAGCTGCCC 
ACCGTGCGGG TGTCTGTGGT GGCCCTCACG CCCTCGGGCC CCGTTGAGGC CTTTGACTTT 
GCTGAGTACC AGCCGGAGCT GTTTGAGAAG TTCCAGAACA TGCGTGCCCA GCACCCCTAC 
GTCCTCACTG CGGACACACT GAAGCTCTAC CAGAACAAGT TCCGGCAGGC CTCGATGGAC 
AACGTGAAGG TGGTCATTCA TTTTACCGAT GGAGTGGATG GGGATCTGGC TGATTTACAA 
AGAGCATCAG AGGAACTCCG ACAAGAAGGT GTCCGAGCTC TGATCTTGGT GGGCCTCGAA 
CGCGTCGCCA ACTTGGAGCA GCTCATGCAG CTGGAGTTCG GGCGAGGGTT CACGTACAAC 
CGGCCCCTGA GGCTGAACTT GCTGGACCTG GACTACGAAC TAGCCGAGCA GCTTGACAAC 
ATTGCTGAGA AAGCCTGCTG TGGGATTCCC TGCAAATGCT CTGGACAAAG AGGAGACCGA 
GGGCCCATAG GCAGCATCGG GCCAAAGGGC ATTCCTGGAG AAGATGGCTA CCGAGGTTAT 
CCTGGTGACG AAGGTGGACC TGGTGAACGC GGTCCTCCTG GCGTGAACGG CACTCAAGGT 
TTCCAGGGCT GCCCCGGGCA GAGAGGAGTC AAGGGCTCTC GTGGATTCCC GGGAGAGAAG 
GGTGAATTAG GAGAAATTGG ACTGGATGGT CTTGATGGTG AAGATGGCGA CAAAGGGCTG 
CCCGGTACTT CTGGAGAGAA AGGGAGTCCA GGAAGGAGGG GCGACAAAGG ACCCAAAGGC 
GACAAAGGGG AGAGAGGCGA TGTCGGGATC AGAGGCGACC CGGGCAATTC AGGACAGGAC 
AGCCAGCAGA GAGGAGCCAA AGGAGAAACC GGAGACATCG GCCCCGTGGG TCTCCCTGGG 
AGAGACGGGG TGTCTGGAGG GCCTGGAGAA CCCGGGAAGA GCGGTGGCTT TGGCCGAAGG 
GGACCAGCGG GAGCTAAGGG CAACCAGGGC GGCCCCGGCC AGCAGGGCCC TGTAGGAGAG 
CAGGGGACCA GAGGCGCACA GGGTCCGCCT GGTCCCACCG GTCCTCCAGG CCTGATTGGT 
GAACAGGGCA TTCCCGGACC TCGGGGAGGC GGGGGAACCG CAGGTGCTCC TGGAGAACGC 
GGCAGAACCG GGCCCCTGGG AAGAAAGGGT GAGCCCGGAG ATCCAGGACC TAAGGGGAGC 
ATCGGGAACC GGGGTCCTCG TGGGGAGACG GGAGATGATG GGCGAGATGG AGTTGGCAGC 
GAAGGTCGCA TAGGCAAGAA GGGAGAAAGA GGATTCCCTG GATACCCAGG TCCAAAGGGC 
AATCCTGGTG AGCCAGGGAC AGACGGACCA CCGGGACCCA AAGGCATCAG AGGCCGAAGG 
GGAAACTCGG GACCTCCAGG GATAGCTGGA CAGAAGGGAG ACCCCGGTTA CCCAGGGCCA 
TCTGGTTACA AAGGCAGCAG AGGCGACTCA ATGGACCAAT GTGCGCTGGT CCAGAGCATC 
AAAGATAAAT GCCCTTGCTG TTACGGGCCC CTGGAGTGCC CCGTCTTCCC GACGGAGCTG 
GCTTTCGCCT TGGACACCTC AGAGGGGGTC ACCCAGGACA GGTTCAGCCA GATGAGGGAG 
GTGGTCCTCA AGATTCTGGA CGACCTGACC ATCGCCGAGA GCAACTGCCC GAGGGGGGCC 
CGCGTGGCCG TGGTCACTTA CAACAACGAG GTTACCACGG AGATCCGCTT TGCCGACTCC 
AAGAAGAAGT CCGTCCTCCT GGACAAGATC AAGAACCTGC AGGTGTCGCT GACCTCCAAG 
CAGCAGAGCC TGGAAACCGC CATGTCCTTT GTGGCCAGAA ACACGTTTAA GCGTGTGCGG 
AGCGGGTTCC TGATGAGGAA GGTGGCGGTG TTCTTCAGCA ACAAGCCCAC GAGGGCGACC 
CCGCAGCTCC GGGAGGCTGT GCTCAAGCTC TCAGATGCTG GGATCACACC CTTGTTCCTC 
ACAAGCCAGG AGGACCGGCA GCTGATCAAT GCCCTACAGA TCAATAACAC AGCGGTGGGA 
CATGCCCTTG TCCTTCCTGC TGGCGGAGAC CTCACAGACT TCTTGAAGAA TGTCCTGACG 
TGTCACGTCT GCTTGGACAT CTGCAACATT GACCCATCCT GTGGATTCGG CAGCTGGAGG 
CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATCGACAT GGCTTTCATC 
TTAGATAGCT CCGAGTCCAC CACCCCGTTC CAGTTCAACG AGATGAGGAA GTACATCGAG 
TACCTGGTCC GACAGCTGGA TGTGAGCCCA GACCCCAAGG CCTCCCAGCA CTTTGCCCGC 
GTGGCGGTGG TGCAGCATGC GCCCTACGAG GCGGTGGACA ACGCCAGTGT GCCGCCTGTG 
AAGGTGGAGT TCTCCCTGAC CGACTACGGC TCCAAAGAGA AGCTGCTGGC CTTCCTTGGC 
AGCAGGATGA CCCAGCTGCA GGGGACCAGG GCTTTGGGCC GCGCCATCGA CTACACCATT 
GAGAACATCT TCGAAAGTGC TCCCAACCCC CGGGACCTGA AGATCGTGGT GCTGATGCTG 
ACGGGCGAGG TGCAGAAGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT ACAGGCCAAG 
TGCAAAGGCT ACTTCTTCGT GATCCTGGGC ATTGGCAGGA AGGTGAATGT CAAGGAGCTG 
TACAGCTCTG CCAGCGAGCC CAACGACGTC TTCTTCAAGC TAGTGGACAA GTCGACCGAG 
CTCAACGAGG AGCCCCTAAT GCGCTTCGGG AGGCTGCTGC CATCCTTCGT CAGCAGTAAA 
GATGCTTTCT ACCTGTCCCC AGATATCAGG AAACAGTGTG ATTGGTTCCA AGCAGACCAA 
CCGGCAAAGA ATCTCGTGCA GTTTGGTTAC AAACAAATAA ATGTTCCTAA TAACGTTACT 
TCAAGTCCTA CATCTAAACT GGTGACCACA ACGAAGCCGG TGATCACCAC CACCAAACCG 
ATCACAGTGG TGAACCTGCC AGCCTCAAAG CCAGCTGCCA CGAGACCTGT GGATGAGAGA 
CCCGCAGCTG GGAGATCCGT GGCCACCAAG ATGGACAGTG CCAGGCCCGT GGCCACCAAG 
ACCGAGGCCA CCAAACCCGT GGCCACCAAG ACCGAGGCCA CCAAGACTGA AGCTGCCAAA 
CCTGTGGTCA CCAAGGCTGA GGCTGCAAAA CCCACGGCCA CCAAGACTGA AGCCACTAAG 
ACCGAGGCTG CAAAGCTCAT GGCCACCAAG ACCGAGGCCA CCAAACCTGT GGCTGCCAGG 
CCCGAGGCGG CAAAGACAGC CACAGCCAGA CCTGCAGCAG CCGCGAAGCC TGCAGCAGCC 
AAACCTGCCC CTGCCAGACC CCCCACTGCT GCCAGATCCA TGGCCACGAG GTCCGAGGCC 
CCCAGGCCCC AGGCAGTCAA ACTGGCTGCC AGCAGGCCCG GCGCTGCTAA GCCCGTGGTG 
AAGGCACCTC GCGAGATCCA TGTGTCTGAG GTCACAGAGA ACAGCGCCAA GCTGCACTGG 
GAGAGGCCCG AGCCCCCCAG CCCTTACTTA TACAACCTCA CCGTCACCTC GGCCCACGAT 
CAGGCCGTGG TTCTGAAGCA GAACCTCACT GTCACGGACC GCGTCATCGG GGGCCTGCTC 
CCCGGGCAGA CTTACCACGT TACGGTGACC TGCTCCCTGA GGTCTCAGGT CAGAGCCATC 
TACCAAGGCA GCTTCAGCAC GAAGAAAATT CAGCCTCCAC CTCCACAAAC AGAGCGATCA 
GCCTCTAGTG CAACCATCAA CCTAGTGGTG AGCGCAGACC GGTTGGCTGG GAGTAAAGCA 
GATATATGTA AGTTGCCAAA GGACGGCGGA ACTTGCAGAG AGTTTGTATT AAAGTGGTAC 
TATGATTCCG TAACTGAAAA CTGCGCCCGA TTCTGGTATG GAGGCTGCGG TGGAAACGAA 
AACAGATTTA ATTCGCAGGA TGAATGTGAA AAGGTTTGCC CTCCTGTGCC CATCAAGCAG 
CCCGGAGTTA TCGCCGCGAT GGGGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_024990267.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846035.1 Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X6, mRNA.

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CloneID	OBa51859
Clone ID Related Accession (Same CDS sequence)	XM_010803870.2 , XM_024990267.1 , XM_010827158.2
Accession Version	XM_024990267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAG AGCAAGCGAG ACATCCTGTT CCTTTTCGAC 
GGCTCAGCCA ACCTCATGGG CCAGTTCACT GCCGCCCGTG ACTTTCTCTA CAAGGTCATC 
GACGAGCTGG ATGTGAAACC TGAGGGGACC CGGGTTGCAG TGGCTCAGTT CAGTGATGAT 
GTCAAGGTGG AGTCCCGTTT TGACCAGCAC CAGAATAAGC CTGAGATCCT GAATCTCGTG 
AAGAGGATGA AGCTCAAGAC AGGCAAAGCC CTCAACCTGG GCTACGCCTT GGATTACGCA 
CAGAGGTACA TCTTTGTGAA GTCCGCTGGA AGCCGGATTG AGGATGGAGT GCTTCAGTTC 
CTGGTGCTGC TGGTGGCGGG AAAGTCATCG GACCGGGTGG ATACACCCGC ACTCAACCTG 
AAGCAGAGCG GGGTGGTGCC CTTCATCCTC CAGGCGAAAA ACGCGGACCC TGCTGAGTTG 
GAGCTGATCG TACCATCCCC AGCCTTTATT TTGGTTGCGG AGTCCCTTCC TAAGATTGGA 
GACCTGCAAC CACAGATTGT GAACCTCTTA AAATCAGTGC AAAATGGGGC ACCGGCACCA 
GTTTCAGTTG AAAAGGATGT GGTGTTCCTG ATTGATGGCT CCGAGGGTGT CAGGAGCGGC 
TTCCCACTGC TGAAGGAGTT CGTCCAGAGA GTGGTGGAGA GCCTGGACGT GGGACCAGAC 
CGGGTGCGTG TGGCCGTGGT GCAGTACAGC GACCGGACCA GACCTGAGTT CTACCTGAAT 
TCCTACATGG ACCAGCAGAG TGTGGTCGGA GCCATCCGTG GGCTGACCCT GCTGGGCGGG 
CCGGCCCCCA ACACAGGGGC TGCCCTGGAG TTCGTCCTGA GGAACATCCT GGTGGGCTCT 
GCTGGGAGCC GCATAGCAGA AGGTGTCCCC CAGCTCCTGA TTGTCCTCAC GGCCGACAGG 
TCTGGGGATG ACGTGAGGGG CCCCTCTGTG GTCCTGAGGA GGGGAGGGGC CGTGCCCATC 
GGCATCGGCA TTGGGAACGC TGACATCACC GAGATGCAGA CCCTCTCCTT CGTCCCGGAC 
TTCGCCGTGG TCATCCCCAC CTTCCGGCAG CTGGGGACCA TCCAACAGGT CATCTCGGAG 
AGAGTGACCC AGCTCAGCCG CGAGGAACTG AGCAGGCTGC AGGCGTCTGT CACCCCCTTG 
ACGACTCCGG TGGTCAGCAG CAAGAGGGAC GTGGTCTTTC TCATCGATGG GTCCCAGAGT 
GCTGGCCCCG AGTTCCAGTA CATCCGAACG CTCATCGAGA GGCTGGTGGA CTACCTGGAT 
GTGGGCTTCG ACACGACCCG GGTGGCGGTC ATCCAGTTCA GCGATGACCC CAGGGTGGAG 
TTCCTGTTGA ACGTCCACTC CAGCAAGGAC GAGGTGCAGA ACGCCGTGCG GCGGCTGCGG 
CCCAAAGGTG GGAGGCAGGT CAACGTTGGG GGTGCCCTGG AGTACGTGGC CAGGAACATC 
TTCAAGAGGC CCCTGGGGAG CCGGATTGAG GAGGGTGTCC CTCAGTTCCT GGTCCTCATT 
TCCTCCGGGA AGTCGGATGA TGAGGTGGAG GACTCGGCCA TAGAGCTCAA GCAGTTTGGC 
GTGGCGCCAC TCACGATCGC CAGGAACGTG GACCAGGAAG AGCTGGTGAA GATCTCGCTG 
AGCCCCGAGT ACGTGTTCTC AGTGAACACC TTCCGGGAGC TCCCCAGCCT GGAGCAGAAA 
CTGCTGACAC CGCTCACCAC CCTGACTGCC GGGCAGATAC AGCAGCTCCT GGCCAGCACG 
CGCTACCCGC CTCCAGCTGT GGAGAGCGAT GCTGCGGACA TTGTCTTCCT CATCGACAGC 
TCTGATGGCG TGAAGCCGGA TGGCATCGCA CACATCCGGG ACTTTGTTAT CAGGATCGTG 
CGGAGACTCA ACGTCGGTCC CAACAAAGTG AGAATTGGGG TCCTGCAGTT CAGCAACGAC 
GTCTTCCCAG AATTTCAACT AAAGACCTAT AAATCCCAGG CCAGCGTGCT GGATGCCATA 
CGTCGCCTGA GGTTCAAAGG GGGGTCTCCT CTCAACACTG GCAAGGCTCT GGAATTCGTG 
GCGAGGAATT ACTTTGTCAA GTCTGCCGGG AGCCGGATAG AAGATGGGGT GCCCCAACAC 
CTGGTTCTAT TTCTGGGTGG AAAATCCCAG GATGATATTT CCAGGTATTC CCAGGTGATA 
AAATCTGCAG GAATTGCGAG CTTAGGGGTT GGAGACCGAA ATATTGACAG AACAGAGCTG 
CAGACCATCA CCAGCGATCC CAGGCTTGTC TTCACGGTGC GGGAGTTCAG AGACCTCCCC 
AGCATAGAAG AAAGGATGGT GAATTCCTTT GGGTCCTCCG GGGTCACCCC TGCACCTCCG 
GGAGTGGACA CACCCTCTCC TTCAAGGCCA GAGAAGAAGA AAGCCGACAT TGTGTTCCTG 
CTGGATGGTT CCATCAATTT CCGGAGAGAC AGTTTCCAGG AAGTGCTCCG TTTTGTGTCA 
GAAATTGTGG ACACGGTTTA TGAAGGGGGC GACTCCATCC AGGTGGGGCT GGTCCAATAC 
AACTCTGACC CCACCGACGA GTTCTTCCTG AAGGACTTCC CCACCAAGCA GCAGATTATC 
GACGCCATAA ACAAGGTGGT CTACAAAGGG GGGAGGCACG CGAACACCAA GGTGGGCCTG 
GAGCACCTGC GGCGGAACCA CTTCGTGCCC GAGGCGGGCA GCCGCCTGGA CCAGAGGGTC 
CCGCAGATCG CCTTTGTGAT CACGGGGGGC AAGTCGGTGG AGGACGCTCA GGAGGCAAGC 
ATGGCGCTCA CGCAGAGAGG CGTCAAGGTG TTTGCGGTGG GCGTAAGGAA CATCGACTCG 
GAGGAGGTTG GGAAGATAGC GTCCAACAGC GCCACGGCCT TCCGGGTGGG GAACGTCCAG 
GAGCTGTCGG AACTGAGCGA GCAGGTTTTG GAAACTCTGC ACGATGCAAT GCATGAGACC 
TTATGTCCAG GCGTGACTGA CGTTTCCAAA GCCTGTAACT TGGATGTGAT TCTGGGCTTC 
GATGGTTCAG GAGATCAGAA TGTCTTTGTG GCTCAGAAGG GCCTCGAGCC CAAGGTGGAC 
ACCATCCTGA GGAGAATCAG TCAGATGCAG AAGATCAGCT GCAGCGGCAG CCAGCTGCCC 
ACCGTGCGGG TGTCTGTGGT GGCCCTCACG CCCTCGGGCC CCGTTGAGGC CTTTGACTTT 
GCTGAGTACC AGCCGGAGCT GTTTGAGAAG TTCCAGAACA TGCGTGCCCA GCACCCCTAC 
GTCCTCACTG CGGACACACT GAAGCTCTAC CAGAACAAGT TCCGGCAGGC CTCGATGGAC 
AACGTGAAGG TGGTCATTCA TTTTACCGAT GGAGTGGATG GGGATCTGGC TGATTTACAA 
AGAGCATCAG AGGAACTCCG ACAAGAAGGT GTCCGAGCTC TGATCTTGGT GGGCCTCGAA 
CGCGTCGCCA ACTTGGAGCA GCTCATGCAG CTGGAGTTCG GGCGAGGGTT CACGTACAAC 
CGGCCCCTGA GGCTGAACTT GCTGGACCTG GACTACGAAC TAGCCGAGCA GCTTGACAAC 
ATTGCTGAGA AAGCCTGCTG TGGGATTCCC TGCAAATGCT CTGGACAAAG AGGAGACCGA 
GGGCCCATAG GCAGCATCGG GCCAAAGGGC ATTCCTGGAG AAGATGGCTA CCGAGGTTAT 
CCTGGTGACG AAGGTGGACC TGGTGAACGC GGTCCTCCTG GCGTGAACGG CACTCAAGGT 
TTCCAGGGCT GCCCCGGGCA GAGAGGAGTC AAGGGCTCTC GTGGATTCCC GGGAGAGAAG 
GGTGAATTAG GAGAAATTGG ACTGGATGGT CTTGATGGTG AAGATGGCGA CAAAGGGCTG 
CCCGGTACTT CTGGAGAGAA AGGGAGTCCA GGAAGGAGGG GCGACAAAGG ACCCAAAGGC 
GACAAAGGGG AGAGAGGCGA TGTCGGGATC AGAGGCGACC CGGGCAATTC AGGACAGGAC 
AGCCAGCAGA GAGGAGCCAA AGGAGAAACC GGAGACATCG GCCCCGTGGG TCTCCCTGGG 
AGAGACGGGG TGTCTGGAGG GCCTGGAGAA CCCGGGAAGA GCGGTGGCTT TGGCCGAAGG 
GGACCAGCGG GAGCTAAGGG CAACCAGGGC GGCCCCGGCC AGCAGGGCCC TGTAGGAGAG 
CAGGGGACCA GAGGCGCACA GGGTCCGCCT GGTCCCACCG GTCCTCCAGG CCTGATTGGT 
GAACAGGGCA TTCCCGGACC TCGGGGAGGC GGGGGAACCG CAGGTGCTCC TGGAGAACGC 
GGCAGAACCG GGCCCCTGGG AAGAAAGGGT GAGCCCGGAG ATCCAGGACC TAAGGGGAGC 
ATCGGGAACC GGGGTCCTCG TGGGGAGACG GGAGATGATG GGCGAGATGG AGTTGGCAGC 
GAAGGTCGCA TAGGCAAGAA GGGAGAAAGA GGATTCCCTG GATACCCAGG TCCAAAGGGC 
AATCCTGGTG AGCCAGGGAC AGACGGACCA CCGGGACCCA AAGGCATCAG AGGCCGAAGG 
GGAAACTCGG GACCTCCAGG GATAGCTGGA CAGAAGGGAG ACCCCGGTTA CCCAGGGCCA 
TCTGGTTACA AAGGCAGCAG AGGCGACTCA ATGGACCAAT GTGCGCTGGT CCAGAGCATC 
AAAGATAAAT GCCCTTGCTG TTACGGGCCC CTGGAGTGCC CCGTCTTCCC GACGGAGCTG 
GCTTTCGCCT TGGACACCTC AGAGGGGGTC ACCCAGGACA GGTTCAGCCA GATGAGGGAG 
GTGGTCCTCA AGATTCTGGA CGACCTGACC ATCGCCGAGA GCAACTGCCC GAGGGGGGCC 
CGCGTGGCCG TGGTCACTTA CAACAACGAG GTTACCACGG AGATCCGCTT TGCCGACTCC 
AAGAAGAAGT CCGTCCTCCT GGACAAGATC AAGAACCTGC AGGTGTCGCT GACCTCCAAG 
CAGCAGAGCC TGGAAACCGC CATGTCCTTT GTGGCCAGAA ACACGTTTAA GCGTGTGCGG 
AGCGGGTTCC TGATGAGGAA GGTGGCGGTG TTCTTCAGCA ACAAGCCCAC GAGGGCGACC 
CCGCAGCTCC GGGAGGCTGT GCTCAAGCTC TCAGATGCTG GGATCACACC CTTGTTCCTC 
ACAAGCCAGG AGGACCGGCA GCTGATCAAT GCCCTACAGA TCAATAACAC AGCGGTGGGA 
CATGCCCTTG TCCTTCCTGC TGGCGGAGAC CTCACAGACT TCTTGAAGAA TGTCCTGACG 
TGTCACGTCT GCTTGGACAT CTGCAACATT GACCCATCCT GTGGATTCGG CAGCTGGAGG 
CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATCGACAT GGCTTTCATC 
TTAGATAGCT CCGAGTCCAC CACCCCGTTC CAGTTCAACG AGATGAGGAA GTACATCGAG 
TACCTGGTCC GACAGCTGGA TGTGAGCCCA GACCCCAAGG CCTCCCAGCA CTTTGCCCGC 
GTGGCGGTGG TGCAGCATGC GCCCTACGAG GCGGTGGACA ACGCCAGTGT GCCGCCTGTG 
AAGGTGGAGT TCTCCCTGAC CGACTACGGC TCCAAAGAGA AGCTGCTGGC CTTCCTTGGC 
AGCAGGATGA CCCAGCTGCA GGGGACCAGG GCTTTGGGCC GCGCCATCGA CTACACCATT 
GAGAACATCT TCGAAAGTGC TCCCAACCCC CGGGACCTGA AGATCGTGGT GCTGATGCTG 
ACGGGCGAGG TGCAGAAGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT ACAGGCCAAG 
TGCAAAGGCT ACTTCTTCGT GATCCTGGGC ATTGGCAGGA AGGTGAATGT CAAGGAGCTG 
TACAGCTCTG CCAGCGAGCC CAACGACGTC TTCTTCAAGC TAGTGGACAA GTCGACCGAG 
CTCAACGAGG AGCCCCTAAT GCGCTTCGGG AGGCTGCTGC CATCCTTCGT CAGCAGTAAA 
GATGCTTTCT ACCTGTCCCC AGATATCAGG AAACAGTGTG ATTGGTTCCA AGCAGACCAA 
CCGGCAAAGA ATCTCGTGCA GTTTGGTTAC AAACAAATAA ATGTTCCTAA TAACGTTACT 
TCAAGTCCTA CATCTAAACT GGTGACCACA ACGAAGCCGG TGATCACCAC CACCAAACCG 
ATCACAGTGG TGAACCTGCC AGCCTCAAAG CCAGCTGCCA CGAGACCTGT GGATGAGAGA 
CCCGCAGCTG GGAGATCCGT GGCCACCAAG ATGGACAGTG CCAGGCCCGT GGCCACCAAG 
ACCGAGGCCA CCAAACCCGT GGCCACCAAG ACCGAGGCCA CCAAGACTGA AGCTGCCAAA 
CCTGTGGTCA CCAAGGCTGA GGCTGCAAAA CCCACGGCCA CCAAGACTGA AGCCACTAAG 
ACCGAGGCTG CAAAGCTCAT GGCCACCAAG ACCGAGGCCA CCAAACCTGT GGCTGCCAGG 
CCCGAGGCGG CAAAGACAGC CACAGCCAGA CCTGCAGCAG CCGCGAAGCC TGCAGCAGCC 
AAACCTGCCC CTGCCAGACC CCCCACTGCT GCCAGATCCA TGGCCACGAG GTCCGAGGCC 
CCCAGGCCCC AGGCAGTCAA ACTGGCTGCC AGCAGGCCCG GCGCTGCTAA GCCCGTGGTG 
AAGGCACCTC GCGAGATCCA TGTGTCTGAG GTCACAGAGA ACAGCGCCAA GCTGCACTGG 
GAGAGGCCCG AGCCCCCCAG CCCTTACTTA TACAACCTCA CCGTCACCTC GGCCCACGAT 
CAGGCCGTGG TTCTGAAGCA GAACCTCACT GTCACGGACC GCGTCATCGG GGGCCTGCTC 
CCCGGGCAGA CTTACCACGT TACGGTGACC TGCTCCCTGA GGTCTCAGGT CAGAGCCATC 
TACCAAGGCA GCTTCAGCAC GAAGAAAATT CAGCCTCCAC CTCCACAAAC AGAGCGATCA 
GCCTCTAGTG CAACCATCAA CCTAGTGGTG AGCGCAGACC GGTTGGCTGG GAGTAAAGCA 
GATATATGTA AGTTGCCAAA GGACGGCGGA ACTTGCAGAG AGTTTGTATT AAAGTGGTAC 
TATGATTCCG TAACTGAAAA CTGCGCCCGA TTCTGGTATG GAGGCTGCGG TGGAAACGAA 
AACAGATTTA ATTCGCAGGA TGAATGTGAA AAGGTTTGCC CTCCTGTGCC CATCAAGCAG 
CCCGGAGTTA TCGCCGCGAT GGGGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846035.1
CDS	275..7564
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_024990267.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990267.1

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAG AGCAAGCGAG ACATCCTGTT CCTTTTCGAC 
GGCTCAGCCA ACCTCATGGG CCAGTTCACT GCCGCCCGTG ACTTTCTCTA CAAGGTCATC 
GACGAGCTGG ATGTGAAACC TGAGGGGACC CGGGTTGCAG TGGCTCAGTT CAGTGATGAT 
GTCAAGGTGG AGTCCCGTTT TGACCAGCAC CAGAATAAGC CTGAGATCCT GAATCTCGTG 
AAGAGGATGA AGCTCAAGAC AGGCAAAGCC CTCAACCTGG GCTACGCCTT GGATTACGCA 
CAGAGGTACA TCTTTGTGAA GTCCGCTGGA AGCCGGATTG AGGATGGAGT GCTTCAGTTC 
CTGGTGCTGC TGGTGGCGGG AAAGTCATCG GACCGGGTGG ATACACCCGC ACTCAACCTG 
AAGCAGAGCG GGGTGGTGCC CTTCATCCTC CAGGCGAAAA ACGCGGACCC TGCTGAGTTG 
GAGCTGATCG TACCATCCCC AGCCTTTATT TTGGTTGCGG AGTCCCTTCC TAAGATTGGA 
GACCTGCAAC CACAGATTGT GAACCTCTTA AAATCAGTGC AAAATGGGGC ACCGGCACCA 
GTTTCAGTTG AAAAGGATGT GGTGTTCCTG ATTGATGGCT CCGAGGGTGT CAGGAGCGGC 
TTCCCACTGC TGAAGGAGTT CGTCCAGAGA GTGGTGGAGA GCCTGGACGT GGGACCAGAC 
CGGGTGCGTG TGGCCGTGGT GCAGTACAGC GACCGGACCA GACCTGAGTT CTACCTGAAT 
TCCTACATGG ACCAGCAGAG TGTGGTCGGA GCCATCCGTG GGCTGACCCT GCTGGGCGGG 
CCGGCCCCCA ACACAGGGGC TGCCCTGGAG TTCGTCCTGA GGAACATCCT GGTGGGCTCT 
GCTGGGAGCC GCATAGCAGA AGGTGTCCCC CAGCTCCTGA TTGTCCTCAC GGCCGACAGG 
TCTGGGGATG ACGTGAGGGG CCCCTCTGTG GTCCTGAGGA GGGGAGGGGC CGTGCCCATC 
GGCATCGGCA TTGGGAACGC TGACATCACC GAGATGCAGA CCCTCTCCTT CGTCCCGGAC 
TTCGCCGTGG TCATCCCCAC CTTCCGGCAG CTGGGGACCA TCCAACAGGT CATCTCGGAG 
AGAGTGACCC AGCTCAGCCG CGAGGAACTG AGCAGGCTGC AGGCGTCTGT CACCCCCTTG 
ACGACTCCGG TGGTCAGCAG CAAGAGGGAC GTGGTCTTTC TCATCGATGG GTCCCAGAGT 
GCTGGCCCCG AGTTCCAGTA CATCCGAACG CTCATCGAGA GGCTGGTGGA CTACCTGGAT 
GTGGGCTTCG ACACGACCCG GGTGGCGGTC ATCCAGTTCA GCGATGACCC CAGGGTGGAG 
TTCCTGTTGA ACGTCCACTC CAGCAAGGAC GAGGTGCAGA ACGCCGTGCG GCGGCTGCGG 
CCCAAAGGTG GGAGGCAGGT CAACGTTGGG GGTGCCCTGG AGTACGTGGC CAGGAACATC 
TTCAAGAGGC CCCTGGGGAG CCGGATTGAG GAGGGTGTCC CTCAGTTCCT GGTCCTCATT 
TCCTCCGGGA AGTCGGATGA TGAGGTGGAG GACTCGGCCA TAGAGCTCAA GCAGTTTGGC 
GTGGCGCCAC TCACGATCGC CAGGAACGTG GACCAGGAAG AGCTGGTGAA GATCTCGCTG 
AGCCCCGAGT ACGTGTTCTC AGTGAACACC TTCCGGGAGC TCCCCAGCCT GGAGCAGAAA 
CTGCTGACAC CGCTCACCAC CCTGACTGCC GGGCAGATAC AGCAGCTCCT GGCCAGCACG 
CGCTACCCGC CTCCAGCTGT GGAGAGCGAT GCTGCGGACA TTGTCTTCCT CATCGACAGC 
TCTGATGGCG TGAAGCCGGA TGGCATCGCA CACATCCGGG ACTTTGTTAT CAGGATCGTG 
CGGAGACTCA ACGTCGGTCC CAACAAAGTG AGAATTGGGG TCCTGCAGTT CAGCAACGAC 
GTCTTCCCAG AATTTCAACT AAAGACCTAT AAATCCCAGG CCAGCGTGCT GGATGCCATA 
CGTCGCCTGA GGTTCAAAGG GGGGTCTCCT CTCAACACTG GCAAGGCTCT GGAATTCGTG 
GCGAGGAATT ACTTTGTCAA GTCTGCCGGG AGCCGGATAG AAGATGGGGT GCCCCAACAC 
CTGGTTCTAT TTCTGGGTGG AAAATCCCAG GATGATATTT CCAGGTATTC CCAGGTGATA 
AAATCTGCAG GAATTGCGAG CTTAGGGGTT GGAGACCGAA ATATTGACAG AACAGAGCTG 
CAGACCATCA CCAGCGATCC CAGGCTTGTC TTCACGGTGC GGGAGTTCAG AGACCTCCCC 
AGCATAGAAG AAAGGATGGT GAATTCCTTT GGGTCCTCCG GGGTCACCCC TGCACCTCCG 
GGAGTGGACA CACCCTCTCC TTCAAGGCCA GAGAAGAAGA AAGCCGACAT TGTGTTCCTG 
CTGGATGGTT CCATCAATTT CCGGAGAGAC AGTTTCCAGG AAGTGCTCCG TTTTGTGTCA 
GAAATTGTGG ACACGGTTTA TGAAGGGGGC GACTCCATCC AGGTGGGGCT GGTCCAATAC 
AACTCTGACC CCACCGACGA GTTCTTCCTG AAGGACTTCC CCACCAAGCA GCAGATTATC 
GACGCCATAA ACAAGGTGGT CTACAAAGGG GGGAGGCACG CGAACACCAA GGTGGGCCTG 
GAGCACCTGC GGCGGAACCA CTTCGTGCCC GAGGCGGGCA GCCGCCTGGA CCAGAGGGTC 
CCGCAGATCG CCTTTGTGAT CACGGGGGGC AAGTCGGTGG AGGACGCTCA GGAGGCAAGC 
ATGGCGCTCA CGCAGAGAGG CGTCAAGGTG TTTGCGGTGG GCGTAAGGAA CATCGACTCG 
GAGGAGGTTG GGAAGATAGC GTCCAACAGC GCCACGGCCT TCCGGGTGGG GAACGTCCAG 
GAGCTGTCGG AACTGAGCGA GCAGGTTTTG GAAACTCTGC ACGATGCAAT GCATGAGACC 
TTATGTCCAG GCGTGACTGA CGTTTCCAAA GCCTGTAACT TGGATGTGAT TCTGGGCTTC 
GATGGTTCAG GAGATCAGAA TGTCTTTGTG GCTCAGAAGG GCCTCGAGCC CAAGGTGGAC 
ACCATCCTGA GGAGAATCAG TCAGATGCAG AAGATCAGCT GCAGCGGCAG CCAGCTGCCC 
ACCGTGCGGG TGTCTGTGGT GGCCCTCACG CCCTCGGGCC CCGTTGAGGC CTTTGACTTT 
GCTGAGTACC AGCCGGAGCT GTTTGAGAAG TTCCAGAACA TGCGTGCCCA GCACCCCTAC 
GTCCTCACTG CGGACACACT GAAGCTCTAC CAGAACAAGT TCCGGCAGGC CTCGATGGAC 
AACGTGAAGG TGGTCATTCA TTTTACCGAT GGAGTGGATG GGGATCTGGC TGATTTACAA 
AGAGCATCAG AGGAACTCCG ACAAGAAGGT GTCCGAGCTC TGATCTTGGT GGGCCTCGAA 
CGCGTCGCCA ACTTGGAGCA GCTCATGCAG CTGGAGTTCG GGCGAGGGTT CACGTACAAC 
CGGCCCCTGA GGCTGAACTT GCTGGACCTG GACTACGAAC TAGCCGAGCA GCTTGACAAC 
ATTGCTGAGA AAGCCTGCTG TGGGATTCCC TGCAAATGCT CTGGACAAAG AGGAGACCGA 
GGGCCCATAG GCAGCATCGG GCCAAAGGGC ATTCCTGGAG AAGATGGCTA CCGAGGTTAT 
CCTGGTGACG AAGGTGGACC TGGTGAACGC GGTCCTCCTG GCGTGAACGG CACTCAAGGT 
TTCCAGGGCT GCCCCGGGCA GAGAGGAGTC AAGGGCTCTC GTGGATTCCC GGGAGAGAAG 
GGTGAATTAG GAGAAATTGG ACTGGATGGT CTTGATGGTG AAGATGGCGA CAAAGGGCTG 
CCCGGTACTT CTGGAGAGAA AGGGAGTCCA GGAAGGAGGG GCGACAAAGG ACCCAAAGGC 
GACAAAGGGG AGAGAGGCGA TGTCGGGATC AGAGGCGACC CGGGCAATTC AGGACAGGAC 
AGCCAGCAGA GAGGAGCCAA AGGAGAAACC GGAGACATCG GCCCCGTGGG TCTCCCTGGG 
AGAGACGGGG TGTCTGGAGG GCCTGGAGAA CCCGGGAAGA GCGGTGGCTT TGGCCGAAGG 
GGACCAGCGG GAGCTAAGGG CAACCAGGGC GGCCCCGGCC AGCAGGGCCC TGTAGGAGAG 
CAGGGGACCA GAGGCGCACA GGGTCCGCCT GGTCCCACCG GTCCTCCAGG CCTGATTGGT 
GAACAGGGCA TTCCCGGACC TCGGGGAGGC GGGGGAACCG CAGGTGCTCC TGGAGAACGC 
GGCAGAACCG GGCCCCTGGG AAGAAAGGGT GAGCCCGGAG ATCCAGGACC TAAGGGGAGC 
ATCGGGAACC GGGGTCCTCG TGGGGAGACG GGAGATGATG GGCGAGATGG AGTTGGCAGC 
GAAGGTCGCA TAGGCAAGAA GGGAGAAAGA GGATTCCCTG GATACCCAGG TCCAAAGGGC 
AATCCTGGTG AGCCAGGGAC AGACGGACCA CCGGGACCCA AAGGCATCAG AGGCCGAAGG 
GGAAACTCGG GACCTCCAGG GATAGCTGGA CAGAAGGGAG ACCCCGGTTA CCCAGGGCCA 
TCTGGTTACA AAGGCAGCAG AGGCGACTCA ATGGACCAAT GTGCGCTGGT CCAGAGCATC 
AAAGATAAAT GCCCTTGCTG TTACGGGCCC CTGGAGTGCC CCGTCTTCCC GACGGAGCTG 
GCTTTCGCCT TGGACACCTC AGAGGGGGTC ACCCAGGACA GGTTCAGCCA GATGAGGGAG 
GTGGTCCTCA AGATTCTGGA CGACCTGACC ATCGCCGAGA GCAACTGCCC GAGGGGGGCC 
CGCGTGGCCG TGGTCACTTA CAACAACGAG GTTACCACGG AGATCCGCTT TGCCGACTCC 
AAGAAGAAGT CCGTCCTCCT GGACAAGATC AAGAACCTGC AGGTGTCGCT GACCTCCAAG 
CAGCAGAGCC TGGAAACCGC CATGTCCTTT GTGGCCAGAA ACACGTTTAA GCGTGTGCGG 
AGCGGGTTCC TGATGAGGAA GGTGGCGGTG TTCTTCAGCA ACAAGCCCAC GAGGGCGACC 
CCGCAGCTCC GGGAGGCTGT GCTCAAGCTC TCAGATGCTG GGATCACACC CTTGTTCCTC 
ACAAGCCAGG AGGACCGGCA GCTGATCAAT GCCCTACAGA TCAATAACAC AGCGGTGGGA 
CATGCCCTTG TCCTTCCTGC TGGCGGAGAC CTCACAGACT TCTTGAAGAA TGTCCTGACG 
TGTCACGTCT GCTTGGACAT CTGCAACATT GACCCATCCT GTGGATTCGG CAGCTGGAGG 
CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATCGACAT GGCTTTCATC 
TTAGATAGCT CCGAGTCCAC CACCCCGTTC CAGTTCAACG AGATGAGGAA GTACATCGAG 
TACCTGGTCC GACAGCTGGA TGTGAGCCCA GACCCCAAGG CCTCCCAGCA CTTTGCCCGC 
GTGGCGGTGG TGCAGCATGC GCCCTACGAG GCGGTGGACA ACGCCAGTGT GCCGCCTGTG 
AAGGTGGAGT TCTCCCTGAC CGACTACGGC TCCAAAGAGA AGCTGCTGGC CTTCCTTGGC 
AGCAGGATGA CCCAGCTGCA GGGGACCAGG GCTTTGGGCC GCGCCATCGA CTACACCATT 
GAGAACATCT TCGAAAGTGC TCCCAACCCC CGGGACCTGA AGATCGTGGT GCTGATGCTG 
ACGGGCGAGG TGCAGAAGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT ACAGGCCAAG 
TGCAAAGGCT ACTTCTTCGT GATCCTGGGC ATTGGCAGGA AGGTGAATGT CAAGGAGCTG 
TACAGCTCTG CCAGCGAGCC CAACGACGTC TTCTTCAAGC TAGTGGACAA GTCGACCGAG 
CTCAACGAGG AGCCCCTAAT GCGCTTCGGG AGGCTGCTGC CATCCTTCGT CAGCAGTAAA 
GATGCTTTCT ACCTGTCCCC AGATATCAGG AAACAGTGTG ATTGGTTCCA AGCAGACCAA 
CCGGCAAAGA ATCTCGTGCA GTTTGGTTAC AAACAAATAA ATGTTCCTAA TAACGTTACT 
TCAAGTCCTA CATCTAAACT GGTGACCACA ACGAAGCCGG TGATCACCAC CACCAAACCG 
ATCACAGTGG TGAACCTGCC AGCCTCAAAG CCAGCTGCCA CGAGACCTGT GGATGAGAGA 
CCCGCAGCTG GGAGATCCGT GGCCACCAAG ATGGACAGTG CCAGGCCCGT GGCCACCAAG 
ACCGAGGCCA CCAAACCCGT GGCCACCAAG ACCGAGGCCA CCAAGACTGA AGCTGCCAAA 
CCTGTGGTCA CCAAGGCTGA GGCTGCAAAA CCCACGGCCA CCAAGACTGA AGCCACTAAG 
ACCGAGGCTG CAAAGCTCAT GGCCACCAAG ACCGAGGCCA CCAAACCTGT GGCTGCCAGG 
CCCGAGGCGG CAAAGACAGC CACAGCCAGA CCTGCAGCAG CCGCGAAGCC TGCAGCAGCC 
AAACCTGCCC CTGCCAGACC CCCCACTGCT GCCAGATCCA TGGCCACGAG GTCCGAGGCC 
CCCAGGCCCC AGGCAGTCAA ACTGGCTGCC AGCAGGCCCG GCGCTGCTAA GCCCGTGGTG 
AAGGCACCTC GCGAGATCCA TGTGTCTGAG GTCACAGAGA ACAGCGCCAA GCTGCACTGG 
GAGAGGCCCG AGCCCCCCAG CCCTTACTTA TACAACCTCA CCGTCACCTC GGCCCACGAT 
CAGGCCGTGG TTCTGAAGCA GAACCTCACT GTCACGGACC GCGTCATCGG GGGCCTGCTC 
CCCGGGCAGA CTTACCACGT TACGGTGACC TGCTCCCTGA GGTCTCAGGT CAGAGCCATC 
TACCAAGGCA GCTTCAGCAC GAAGAAAATT CAGCCTCCAC CTCCACAAAC AGAGCGATCA 
GCCTCTAGTG CAACCATCAA CCTAGTGGTG AGCGCAGACC GGTTGGCTGG GAGTAAAGCA 
GATATATGTA AGTTGCCAAA GGACGGCGGA ACTTGCAGAG AGTTTGTATT AAAGTGGTAC 
TATGATTCCG TAACTGAAAA CTGCGCCCGA TTCTGGTATG GAGGCTGCGG TGGAAACGAA 
AACAGATTTA ATTCGCAGGA TGAATGTGAA AAGGTTTGCC CTCCTGTGCC CATCAAGCAG 
CCCGGAGTTA TCGCCGCGAT GGGGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_010827158.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825460.1 Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X6, mRNA.

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CloneID	OBa51859
Clone ID Related Accession (Same CDS sequence)	XM_010803870.2 , XM_024990267.1 , XM_010827158.2
Accession Version	XM_010827158.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644673.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAG AGCAAGCGAG ACATCCTGTT CCTTTTCGAC 
GGCTCAGCCA ACCTCATGGG CCAGTTCACT GCCGCCCGTG ACTTTCTCTA CAAGGTCATC 
GACGAGCTGG ATGTGAAACC TGAGGGGACC CGGGTTGCAG TGGCTCAGTT CAGTGATGAT 
GTCAAGGTGG AGTCCCGTTT TGACCAGCAC CAGAATAAGC CTGAGATCCT GAATCTCGTG 
AAGAGGATGA AGCTCAAGAC AGGCAAAGCC CTCAACCTGG GCTACGCCTT GGATTACGCA 
CAGAGGTACA TCTTTGTGAA GTCCGCTGGA AGCCGGATTG AGGATGGAGT GCTTCAGTTC 
CTGGTGCTGC TGGTGGCGGG AAAGTCATCG GACCGGGTGG ATACACCCGC ACTCAACCTG 
AAGCAGAGCG GGGTGGTGCC CTTCATCCTC CAGGCGAAAA ACGCGGACCC TGCTGAGTTG 
GAGCTGATCG TACCATCCCC AGCCTTTATT TTGGTTGCGG AGTCCCTTCC TAAGATTGGA 
GACCTGCAAC CACAGATTGT GAACCTCTTA AAATCAGTGC AAAATGGGGC ACCGGCACCA 
GTTTCAGTTG AAAAGGATGT GGTGTTCCTG ATTGATGGCT CCGAGGGTGT CAGGAGCGGC 
TTCCCACTGC TGAAGGAGTT CGTCCAGAGA GTGGTGGAGA GCCTGGACGT GGGACCAGAC 
CGGGTGCGTG TGGCCGTGGT GCAGTACAGC GACCGGACCA GACCTGAGTT CTACCTGAAT 
TCCTACATGG ACCAGCAGAG TGTGGTCGGA GCCATCCGTG GGCTGACCCT GCTGGGCGGG 
CCGGCCCCCA ACACAGGGGC TGCCCTGGAG TTCGTCCTGA GGAACATCCT GGTGGGCTCT 
GCTGGGAGCC GCATAGCAGA AGGTGTCCCC CAGCTCCTGA TTGTCCTCAC GGCCGACAGG 
TCTGGGGATG ACGTGAGGGG CCCCTCTGTG GTCCTGAGGA GGGGAGGGGC CGTGCCCATC 
GGCATCGGCA TTGGGAACGC TGACATCACC GAGATGCAGA CCCTCTCCTT CGTCCCGGAC 
TTCGCCGTGG TCATCCCCAC CTTCCGGCAG CTGGGGACCA TCCAACAGGT CATCTCGGAG 
AGAGTGACCC AGCTCAGCCG CGAGGAACTG AGCAGGCTGC AGGCGTCTGT CACCCCCTTG 
ACGACTCCGG TGGTCAGCAG CAAGAGGGAC GTGGTCTTTC TCATCGATGG GTCCCAGAGT 
GCTGGCCCCG AGTTCCAGTA CATCCGAACG CTCATCGAGA GGCTGGTGGA CTACCTGGAT 
GTGGGCTTCG ACACGACCCG GGTGGCGGTC ATCCAGTTCA GCGATGACCC CAGGGTGGAG 
TTCCTGTTGA ACGTCCACTC CAGCAAGGAC GAGGTGCAGA ACGCCGTGCG GCGGCTGCGG 
CCCAAAGGTG GGAGGCAGGT CAACGTTGGG GGTGCCCTGG AGTACGTGGC CAGGAACATC 
TTCAAGAGGC CCCTGGGGAG CCGGATTGAG GAGGGTGTCC CTCAGTTCCT GGTCCTCATT 
TCCTCCGGGA AGTCGGATGA TGAGGTGGAG GACTCGGCCA TAGAGCTCAA GCAGTTTGGC 
GTGGCGCCAC TCACGATCGC CAGGAACGTG GACCAGGAAG AGCTGGTGAA GATCTCGCTG 
AGCCCCGAGT ACGTGTTCTC AGTGAACACC TTCCGGGAGC TCCCCAGCCT GGAGCAGAAA 
CTGCTGACAC CGCTCACCAC CCTGACTGCC GGGCAGATAC AGCAGCTCCT GGCCAGCACG 
CGCTACCCGC CTCCAGCTGT GGAGAGCGAT GCTGCGGACA TTGTCTTCCT CATCGACAGC 
TCTGATGGCG TGAAGCCGGA TGGCATCGCA CACATCCGGG ACTTTGTTAT CAGGATCGTG 
CGGAGACTCA ACGTCGGTCC CAACAAAGTG AGAATTGGGG TCCTGCAGTT CAGCAACGAC 
GTCTTCCCAG AATTTCAACT AAAGACCTAT AAATCCCAGG CCAGCGTGCT GGATGCCATA 
CGTCGCCTGA GGTTCAAAGG GGGGTCTCCT CTCAACACTG GCAAGGCTCT GGAATTCGTG 
GCGAGGAATT ACTTTGTCAA GTCTGCCGGG AGCCGGATAG AAGATGGGGT GCCCCAACAC 
CTGGTTCTAT TTCTGGGTGG AAAATCCCAG GATGATATTT CCAGGTATTC CCAGGTGATA 
AAATCTGCAG GAATTGCGAG CTTAGGGGTT GGAGACCGAA ATATTGACAG AACAGAGCTG 
CAGACCATCA CCAGCGATCC CAGGCTTGTC TTCACGGTGC GGGAGTTCAG AGACCTCCCC 
AGCATAGAAG AAAGGATGGT GAATTCCTTT GGGTCCTCCG GGGTCACCCC TGCACCTCCG 
GGAGTGGACA CACCCTCTCC TTCAAGGCCA GAGAAGAAGA AAGCCGACAT TGTGTTCCTG 
CTGGATGGTT CCATCAATTT CCGGAGAGAC AGTTTCCAGG AAGTGCTCCG TTTTGTGTCA 
GAAATTGTGG ACACGGTTTA TGAAGGGGGC GACTCCATCC AGGTGGGGCT GGTCCAATAC 
AACTCTGACC CCACCGACGA GTTCTTCCTG AAGGACTTCC CCACCAAGCA GCAGATTATC 
GACGCCATAA ACAAGGTGGT CTACAAAGGG GGGAGGCACG CGAACACCAA GGTGGGCCTG 
GAGCACCTGC GGCGGAACCA CTTCGTGCCC GAGGCGGGCA GCCGCCTGGA CCAGAGGGTC 
CCGCAGATCG CCTTTGTGAT CACGGGGGGC AAGTCGGTGG AGGACGCTCA GGAGGCAAGC 
ATGGCGCTCA CGCAGAGAGG CGTCAAGGTG TTTGCGGTGG GCGTAAGGAA CATCGACTCG 
GAGGAGGTTG GGAAGATAGC GTCCAACAGC GCCACGGCCT TCCGGGTGGG GAACGTCCAG 
GAGCTGTCGG AACTGAGCGA GCAGGTTTTG GAAACTCTGC ACGATGCAAT GCATGAGACC 
TTATGTCCAG GCGTGACTGA CGTTTCCAAA GCCTGTAACT TGGATGTGAT TCTGGGCTTC 
GATGGTTCAG GAGATCAGAA TGTCTTTGTG GCTCAGAAGG GCCTCGAGCC CAAGGTGGAC 
ACCATCCTGA GGAGAATCAG TCAGATGCAG AAGATCAGCT GCAGCGGCAG CCAGCTGCCC 
ACCGTGCGGG TGTCTGTGGT GGCCCTCACG CCCTCGGGCC CCGTTGAGGC CTTTGACTTT 
GCTGAGTACC AGCCGGAGCT GTTTGAGAAG TTCCAGAACA TGCGTGCCCA GCACCCCTAC 
GTCCTCACTG CGGACACACT GAAGCTCTAC CAGAACAAGT TCCGGCAGGC CTCGATGGAC 
AACGTGAAGG TGGTCATTCA TTTTACCGAT GGAGTGGATG GGGATCTGGC TGATTTACAA 
AGAGCATCAG AGGAACTCCG ACAAGAAGGT GTCCGAGCTC TGATCTTGGT GGGCCTCGAA 
CGCGTCGCCA ACTTGGAGCA GCTCATGCAG CTGGAGTTCG GGCGAGGGTT CACGTACAAC 
CGGCCCCTGA GGCTGAACTT GCTGGACCTG GACTACGAAC TAGCCGAGCA GCTTGACAAC 
ATTGCTGAGA AAGCCTGCTG TGGGATTCCC TGCAAATGCT CTGGACAAAG AGGAGACCGA 
GGGCCCATAG GCAGCATCGG GCCAAAGGGC ATTCCTGGAG AAGATGGCTA CCGAGGTTAT 
CCTGGTGACG AAGGTGGACC TGGTGAACGC GGTCCTCCTG GCGTGAACGG CACTCAAGGT 
TTCCAGGGCT GCCCCGGGCA GAGAGGAGTC AAGGGCTCTC GTGGATTCCC GGGAGAGAAG 
GGTGAATTAG GAGAAATTGG ACTGGATGGT CTTGATGGTG AAGATGGCGA CAAAGGGCTG 
CCCGGTACTT CTGGAGAGAA AGGGAGTCCA GGAAGGAGGG GCGACAAAGG ACCCAAAGGC 
GACAAAGGGG AGAGAGGCGA TGTCGGGATC AGAGGCGACC CGGGCAATTC AGGACAGGAC 
AGCCAGCAGA GAGGAGCCAA AGGAGAAACC GGAGACATCG GCCCCGTGGG TCTCCCTGGG 
AGAGACGGGG TGTCTGGAGG GCCTGGAGAA CCCGGGAAGA GCGGTGGCTT TGGCCGAAGG 
GGACCAGCGG GAGCTAAGGG CAACCAGGGC GGCCCCGGCC AGCAGGGCCC TGTAGGAGAG 
CAGGGGACCA GAGGCGCACA GGGTCCGCCT GGTCCCACCG GTCCTCCAGG CCTGATTGGT 
GAACAGGGCA TTCCCGGACC TCGGGGAGGC GGGGGAACCG CAGGTGCTCC TGGAGAACGC 
GGCAGAACCG GGCCCCTGGG AAGAAAGGGT GAGCCCGGAG ATCCAGGACC TAAGGGGAGC 
ATCGGGAACC GGGGTCCTCG TGGGGAGACG GGAGATGATG GGCGAGATGG AGTTGGCAGC 
GAAGGTCGCA TAGGCAAGAA GGGAGAAAGA GGATTCCCTG GATACCCAGG TCCAAAGGGC 
AATCCTGGTG AGCCAGGGAC AGACGGACCA CCGGGACCCA AAGGCATCAG AGGCCGAAGG 
GGAAACTCGG GACCTCCAGG GATAGCTGGA CAGAAGGGAG ACCCCGGTTA CCCAGGGCCA 
TCTGGTTACA AAGGCAGCAG AGGCGACTCA ATGGACCAAT GTGCGCTGGT CCAGAGCATC 
AAAGATAAAT GCCCTTGCTG TTACGGGCCC CTGGAGTGCC CCGTCTTCCC GACGGAGCTG 
GCTTTCGCCT TGGACACCTC AGAGGGGGTC ACCCAGGACA GGTTCAGCCA GATGAGGGAG 
GTGGTCCTCA AGATTCTGGA CGACCTGACC ATCGCCGAGA GCAACTGCCC GAGGGGGGCC 
CGCGTGGCCG TGGTCACTTA CAACAACGAG GTTACCACGG AGATCCGCTT TGCCGACTCC 
AAGAAGAAGT CCGTCCTCCT GGACAAGATC AAGAACCTGC AGGTGTCGCT GACCTCCAAG 
CAGCAGAGCC TGGAAACCGC CATGTCCTTT GTGGCCAGAA ACACGTTTAA GCGTGTGCGG 
AGCGGGTTCC TGATGAGGAA GGTGGCGGTG TTCTTCAGCA ACAAGCCCAC GAGGGCGACC 
CCGCAGCTCC GGGAGGCTGT GCTCAAGCTC TCAGATGCTG GGATCACACC CTTGTTCCTC 
ACAAGCCAGG AGGACCGGCA GCTGATCAAT GCCCTACAGA TCAATAACAC AGCGGTGGGA 
CATGCCCTTG TCCTTCCTGC TGGCGGAGAC CTCACAGACT TCTTGAAGAA TGTCCTGACG 
TGTCACGTCT GCTTGGACAT CTGCAACATT GACCCATCCT GTGGATTCGG CAGCTGGAGG 
CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATCGACAT GGCTTTCATC 
TTAGATAGCT CCGAGTCCAC CACCCCGTTC CAGTTCAACG AGATGAGGAA GTACATCGAG 
TACCTGGTCC GACAGCTGGA TGTGAGCCCA GACCCCAAGG CCTCCCAGCA CTTTGCCCGC 
GTGGCGGTGG TGCAGCATGC GCCCTACGAG GCGGTGGACA ACGCCAGTGT GCCGCCTGTG 
AAGGTGGAGT TCTCCCTGAC CGACTACGGC TCCAAAGAGA AGCTGCTGGC CTTCCTTGGC 
AGCAGGATGA CCCAGCTGCA GGGGACCAGG GCTTTGGGCC GCGCCATCGA CTACACCATT 
GAGAACATCT TCGAAAGTGC TCCCAACCCC CGGGACCTGA AGATCGTGGT GCTGATGCTG 
ACGGGCGAGG TGCAGAAGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT ACAGGCCAAG 
TGCAAAGGCT ACTTCTTCGT GATCCTGGGC ATTGGCAGGA AGGTGAATGT CAAGGAGCTG 
TACAGCTCTG CCAGCGAGCC CAACGACGTC TTCTTCAAGC TAGTGGACAA GTCGACCGAG 
CTCAACGAGG AGCCCCTAAT GCGCTTCGGG AGGCTGCTGC CATCCTTCGT CAGCAGTAAA 
GATGCTTTCT ACCTGTCCCC AGATATCAGG AAACAGTGTG ATTGGTTCCA AGCAGACCAA 
CCGGCAAAGA ATCTCGTGCA GTTTGGTTAC AAACAAATAA ATGTTCCTAA TAACGTTACT 
TCAAGTCCTA CATCTAAACT GGTGACCACA ACGAAGCCGG TGATCACCAC CACCAAACCG 
ATCACAGTGG TGAACCTGCC AGCCTCAAAG CCAGCTGCCA CGAGACCTGT GGATGAGAGA 
CCCGCAGCTG GGAGATCCGT GGCCACCAAG ATGGACAGTG CCAGGCCCGT GGCCACCAAG 
ACCGAGGCCA CCAAACCCGT GGCCACCAAG ACCGAGGCCA CCAAGACTGA AGCTGCCAAA 
CCTGTGGTCA CCAAGGCTGA GGCTGCAAAA CCCACGGCCA CCAAGACTGA AGCCACTAAG 
ACCGAGGCTG CAAAGCTCAT GGCCACCAAG ACCGAGGCCA CCAAACCTGT GGCTGCCAGG 
CCCGAGGCGG CAAAGACAGC CACAGCCAGA CCTGCAGCAG CCGCGAAGCC TGCAGCAGCC 
AAACCTGCCC CTGCCAGACC CCCCACTGCT GCCAGATCCA TGGCCACGAG GTCCGAGGCC 
CCCAGGCCCC AGGCAGTCAA ACTGGCTGCC AGCAGGCCCG GCGCTGCTAA GCCCGTGGTG 
AAGGCACCTC GCGAGATCCA TGTGTCTGAG GTCACAGAGA ACAGCGCCAA GCTGCACTGG 
GAGAGGCCCG AGCCCCCCAG CCCTTACTTA TACAACCTCA CCGTCACCTC GGCCCACGAT 
CAGGCCGTGG TTCTGAAGCA GAACCTCACT GTCACGGACC GCGTCATCGG GGGCCTGCTC 
CCCGGGCAGA CTTACCACGT TACGGTGACC TGCTCCCTGA GGTCTCAGGT CAGAGCCATC 
TACCAAGGCA GCTTCAGCAC GAAGAAAATT CAGCCTCCAC CTCCACAAAC AGAGCGATCA 
GCCTCTAGTG CAACCATCAA CCTAGTGGTG AGCGCAGACC GGTTGGCTGG GAGTAAAGCA 
GATATATGTA AGTTGCCAAA GGACGGCGGA ACTTGCAGAG AGTTTGTATT AAAGTGGTAC 
TATGATTCCG TAACTGAAAA CTGCGCCCGA TTCTGGTATG GAGGCTGCGG TGGAAACGAA 
AACAGATTTA ATTCGCAGGA TGAATGTGAA AAGGTTTGCC CTCCTGTGCC CATCAAGCAG 
CCCGGAGTTA TCGCCGCGAT GGGGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825460.1
CDS	338..7627
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_010827158.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010827158.2

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAG AGCAAGCGAG ACATCCTGTT CCTTTTCGAC 
GGCTCAGCCA ACCTCATGGG CCAGTTCACT GCCGCCCGTG ACTTTCTCTA CAAGGTCATC 
GACGAGCTGG ATGTGAAACC TGAGGGGACC CGGGTTGCAG TGGCTCAGTT CAGTGATGAT 
GTCAAGGTGG AGTCCCGTTT TGACCAGCAC CAGAATAAGC CTGAGATCCT GAATCTCGTG 
AAGAGGATGA AGCTCAAGAC AGGCAAAGCC CTCAACCTGG GCTACGCCTT GGATTACGCA 
CAGAGGTACA TCTTTGTGAA GTCCGCTGGA AGCCGGATTG AGGATGGAGT GCTTCAGTTC 
CTGGTGCTGC TGGTGGCGGG AAAGTCATCG GACCGGGTGG ATACACCCGC ACTCAACCTG 
AAGCAGAGCG GGGTGGTGCC CTTCATCCTC CAGGCGAAAA ACGCGGACCC TGCTGAGTTG 
GAGCTGATCG TACCATCCCC AGCCTTTATT TTGGTTGCGG AGTCCCTTCC TAAGATTGGA 
GACCTGCAAC CACAGATTGT GAACCTCTTA AAATCAGTGC AAAATGGGGC ACCGGCACCA 
GTTTCAGTTG AAAAGGATGT GGTGTTCCTG ATTGATGGCT CCGAGGGTGT CAGGAGCGGC 
TTCCCACTGC TGAAGGAGTT CGTCCAGAGA GTGGTGGAGA GCCTGGACGT GGGACCAGAC 
CGGGTGCGTG TGGCCGTGGT GCAGTACAGC GACCGGACCA GACCTGAGTT CTACCTGAAT 
TCCTACATGG ACCAGCAGAG TGTGGTCGGA GCCATCCGTG GGCTGACCCT GCTGGGCGGG 
CCGGCCCCCA ACACAGGGGC TGCCCTGGAG TTCGTCCTGA GGAACATCCT GGTGGGCTCT 
GCTGGGAGCC GCATAGCAGA AGGTGTCCCC CAGCTCCTGA TTGTCCTCAC GGCCGACAGG 
TCTGGGGATG ACGTGAGGGG CCCCTCTGTG GTCCTGAGGA GGGGAGGGGC CGTGCCCATC 
GGCATCGGCA TTGGGAACGC TGACATCACC GAGATGCAGA CCCTCTCCTT CGTCCCGGAC 
TTCGCCGTGG TCATCCCCAC CTTCCGGCAG CTGGGGACCA TCCAACAGGT CATCTCGGAG 
AGAGTGACCC AGCTCAGCCG CGAGGAACTG AGCAGGCTGC AGGCGTCTGT CACCCCCTTG 
ACGACTCCGG TGGTCAGCAG CAAGAGGGAC GTGGTCTTTC TCATCGATGG GTCCCAGAGT 
GCTGGCCCCG AGTTCCAGTA CATCCGAACG CTCATCGAGA GGCTGGTGGA CTACCTGGAT 
GTGGGCTTCG ACACGACCCG GGTGGCGGTC ATCCAGTTCA GCGATGACCC CAGGGTGGAG 
TTCCTGTTGA ACGTCCACTC CAGCAAGGAC GAGGTGCAGA ACGCCGTGCG GCGGCTGCGG 
CCCAAAGGTG GGAGGCAGGT CAACGTTGGG GGTGCCCTGG AGTACGTGGC CAGGAACATC 
TTCAAGAGGC CCCTGGGGAG CCGGATTGAG GAGGGTGTCC CTCAGTTCCT GGTCCTCATT 
TCCTCCGGGA AGTCGGATGA TGAGGTGGAG GACTCGGCCA TAGAGCTCAA GCAGTTTGGC 
GTGGCGCCAC TCACGATCGC CAGGAACGTG GACCAGGAAG AGCTGGTGAA GATCTCGCTG 
AGCCCCGAGT ACGTGTTCTC AGTGAACACC TTCCGGGAGC TCCCCAGCCT GGAGCAGAAA 
CTGCTGACAC CGCTCACCAC CCTGACTGCC GGGCAGATAC AGCAGCTCCT GGCCAGCACG 
CGCTACCCGC CTCCAGCTGT GGAGAGCGAT GCTGCGGACA TTGTCTTCCT CATCGACAGC 
TCTGATGGCG TGAAGCCGGA TGGCATCGCA CACATCCGGG ACTTTGTTAT CAGGATCGTG 
CGGAGACTCA ACGTCGGTCC CAACAAAGTG AGAATTGGGG TCCTGCAGTT CAGCAACGAC 
GTCTTCCCAG AATTTCAACT AAAGACCTAT AAATCCCAGG CCAGCGTGCT GGATGCCATA 
CGTCGCCTGA GGTTCAAAGG GGGGTCTCCT CTCAACACTG GCAAGGCTCT GGAATTCGTG 
GCGAGGAATT ACTTTGTCAA GTCTGCCGGG AGCCGGATAG AAGATGGGGT GCCCCAACAC 
CTGGTTCTAT TTCTGGGTGG AAAATCCCAG GATGATATTT CCAGGTATTC CCAGGTGATA 
AAATCTGCAG GAATTGCGAG CTTAGGGGTT GGAGACCGAA ATATTGACAG AACAGAGCTG 
CAGACCATCA CCAGCGATCC CAGGCTTGTC TTCACGGTGC GGGAGTTCAG AGACCTCCCC 
AGCATAGAAG AAAGGATGGT GAATTCCTTT GGGTCCTCCG GGGTCACCCC TGCACCTCCG 
GGAGTGGACA CACCCTCTCC TTCAAGGCCA GAGAAGAAGA AAGCCGACAT TGTGTTCCTG 
CTGGATGGTT CCATCAATTT CCGGAGAGAC AGTTTCCAGG AAGTGCTCCG TTTTGTGTCA 
GAAATTGTGG ACACGGTTTA TGAAGGGGGC GACTCCATCC AGGTGGGGCT GGTCCAATAC 
AACTCTGACC CCACCGACGA GTTCTTCCTG AAGGACTTCC CCACCAAGCA GCAGATTATC 
GACGCCATAA ACAAGGTGGT CTACAAAGGG GGGAGGCACG CGAACACCAA GGTGGGCCTG 
GAGCACCTGC GGCGGAACCA CTTCGTGCCC GAGGCGGGCA GCCGCCTGGA CCAGAGGGTC 
CCGCAGATCG CCTTTGTGAT CACGGGGGGC AAGTCGGTGG AGGACGCTCA GGAGGCAAGC 
ATGGCGCTCA CGCAGAGAGG CGTCAAGGTG TTTGCGGTGG GCGTAAGGAA CATCGACTCG 
GAGGAGGTTG GGAAGATAGC GTCCAACAGC GCCACGGCCT TCCGGGTGGG GAACGTCCAG 
GAGCTGTCGG AACTGAGCGA GCAGGTTTTG GAAACTCTGC ACGATGCAAT GCATGAGACC 
TTATGTCCAG GCGTGACTGA CGTTTCCAAA GCCTGTAACT TGGATGTGAT TCTGGGCTTC 
GATGGTTCAG GAGATCAGAA TGTCTTTGTG GCTCAGAAGG GCCTCGAGCC CAAGGTGGAC 
ACCATCCTGA GGAGAATCAG TCAGATGCAG AAGATCAGCT GCAGCGGCAG CCAGCTGCCC 
ACCGTGCGGG TGTCTGTGGT GGCCCTCACG CCCTCGGGCC CCGTTGAGGC CTTTGACTTT 
GCTGAGTACC AGCCGGAGCT GTTTGAGAAG TTCCAGAACA TGCGTGCCCA GCACCCCTAC 
GTCCTCACTG CGGACACACT GAAGCTCTAC CAGAACAAGT TCCGGCAGGC CTCGATGGAC 
AACGTGAAGG TGGTCATTCA TTTTACCGAT GGAGTGGATG GGGATCTGGC TGATTTACAA 
AGAGCATCAG AGGAACTCCG ACAAGAAGGT GTCCGAGCTC TGATCTTGGT GGGCCTCGAA 
CGCGTCGCCA ACTTGGAGCA GCTCATGCAG CTGGAGTTCG GGCGAGGGTT CACGTACAAC 
CGGCCCCTGA GGCTGAACTT GCTGGACCTG GACTACGAAC TAGCCGAGCA GCTTGACAAC 
ATTGCTGAGA AAGCCTGCTG TGGGATTCCC TGCAAATGCT CTGGACAAAG AGGAGACCGA 
GGGCCCATAG GCAGCATCGG GCCAAAGGGC ATTCCTGGAG AAGATGGCTA CCGAGGTTAT 
CCTGGTGACG AAGGTGGACC TGGTGAACGC GGTCCTCCTG GCGTGAACGG CACTCAAGGT 
TTCCAGGGCT GCCCCGGGCA GAGAGGAGTC AAGGGCTCTC GTGGATTCCC GGGAGAGAAG 
GGTGAATTAG GAGAAATTGG ACTGGATGGT CTTGATGGTG AAGATGGCGA CAAAGGGCTG 
CCCGGTACTT CTGGAGAGAA AGGGAGTCCA GGAAGGAGGG GCGACAAAGG ACCCAAAGGC 
GACAAAGGGG AGAGAGGCGA TGTCGGGATC AGAGGCGACC CGGGCAATTC AGGACAGGAC 
AGCCAGCAGA GAGGAGCCAA AGGAGAAACC GGAGACATCG GCCCCGTGGG TCTCCCTGGG 
AGAGACGGGG TGTCTGGAGG GCCTGGAGAA CCCGGGAAGA GCGGTGGCTT TGGCCGAAGG 
GGACCAGCGG GAGCTAAGGG CAACCAGGGC GGCCCCGGCC AGCAGGGCCC TGTAGGAGAG 
CAGGGGACCA GAGGCGCACA GGGTCCGCCT GGTCCCACCG GTCCTCCAGG CCTGATTGGT 
GAACAGGGCA TTCCCGGACC TCGGGGAGGC GGGGGAACCG CAGGTGCTCC TGGAGAACGC 
GGCAGAACCG GGCCCCTGGG AAGAAAGGGT GAGCCCGGAG ATCCAGGACC TAAGGGGAGC 
ATCGGGAACC GGGGTCCTCG TGGGGAGACG GGAGATGATG GGCGAGATGG AGTTGGCAGC 
GAAGGTCGCA TAGGCAAGAA GGGAGAAAGA GGATTCCCTG GATACCCAGG TCCAAAGGGC 
AATCCTGGTG AGCCAGGGAC AGACGGACCA CCGGGACCCA AAGGCATCAG AGGCCGAAGG 
GGAAACTCGG GACCTCCAGG GATAGCTGGA CAGAAGGGAG ACCCCGGTTA CCCAGGGCCA 
TCTGGTTACA AAGGCAGCAG AGGCGACTCA ATGGACCAAT GTGCGCTGGT CCAGAGCATC 
AAAGATAAAT GCCCTTGCTG TTACGGGCCC CTGGAGTGCC CCGTCTTCCC GACGGAGCTG 
GCTTTCGCCT TGGACACCTC AGAGGGGGTC ACCCAGGACA GGTTCAGCCA GATGAGGGAG 
GTGGTCCTCA AGATTCTGGA CGACCTGACC ATCGCCGAGA GCAACTGCCC GAGGGGGGCC 
CGCGTGGCCG TGGTCACTTA CAACAACGAG GTTACCACGG AGATCCGCTT TGCCGACTCC 
AAGAAGAAGT CCGTCCTCCT GGACAAGATC AAGAACCTGC AGGTGTCGCT GACCTCCAAG 
CAGCAGAGCC TGGAAACCGC CATGTCCTTT GTGGCCAGAA ACACGTTTAA GCGTGTGCGG 
AGCGGGTTCC TGATGAGGAA GGTGGCGGTG TTCTTCAGCA ACAAGCCCAC GAGGGCGACC 
CCGCAGCTCC GGGAGGCTGT GCTCAAGCTC TCAGATGCTG GGATCACACC CTTGTTCCTC 
ACAAGCCAGG AGGACCGGCA GCTGATCAAT GCCCTACAGA TCAATAACAC AGCGGTGGGA 
CATGCCCTTG TCCTTCCTGC TGGCGGAGAC CTCACAGACT TCTTGAAGAA TGTCCTGACG 
TGTCACGTCT GCTTGGACAT CTGCAACATT GACCCATCCT GTGGATTCGG CAGCTGGAGG 
CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATCGACAT GGCTTTCATC 
TTAGATAGCT CCGAGTCCAC CACCCCGTTC CAGTTCAACG AGATGAGGAA GTACATCGAG 
TACCTGGTCC GACAGCTGGA TGTGAGCCCA GACCCCAAGG CCTCCCAGCA CTTTGCCCGC 
GTGGCGGTGG TGCAGCATGC GCCCTACGAG GCGGTGGACA ACGCCAGTGT GCCGCCTGTG 
AAGGTGGAGT TCTCCCTGAC CGACTACGGC TCCAAAGAGA AGCTGCTGGC CTTCCTTGGC 
AGCAGGATGA CCCAGCTGCA GGGGACCAGG GCTTTGGGCC GCGCCATCGA CTACACCATT 
GAGAACATCT TCGAAAGTGC TCCCAACCCC CGGGACCTGA AGATCGTGGT GCTGATGCTG 
ACGGGCGAGG TGCAGAAGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT ACAGGCCAAG 
TGCAAAGGCT ACTTCTTCGT GATCCTGGGC ATTGGCAGGA AGGTGAATGT CAAGGAGCTG 
TACAGCTCTG CCAGCGAGCC CAACGACGTC TTCTTCAAGC TAGTGGACAA GTCGACCGAG 
CTCAACGAGG AGCCCCTAAT GCGCTTCGGG AGGCTGCTGC CATCCTTCGT CAGCAGTAAA 
GATGCTTTCT ACCTGTCCCC AGATATCAGG AAACAGTGTG ATTGGTTCCA AGCAGACCAA 
CCGGCAAAGA ATCTCGTGCA GTTTGGTTAC AAACAAATAA ATGTTCCTAA TAACGTTACT 
TCAAGTCCTA CATCTAAACT GGTGACCACA ACGAAGCCGG TGATCACCAC CACCAAACCG 
ATCACAGTGG TGAACCTGCC AGCCTCAAAG CCAGCTGCCA CGAGACCTGT GGATGAGAGA 
CCCGCAGCTG GGAGATCCGT GGCCACCAAG ATGGACAGTG CCAGGCCCGT GGCCACCAAG 
ACCGAGGCCA CCAAACCCGT GGCCACCAAG ACCGAGGCCA CCAAGACTGA AGCTGCCAAA 
CCTGTGGTCA CCAAGGCTGA GGCTGCAAAA CCCACGGCCA CCAAGACTGA AGCCACTAAG 
ACCGAGGCTG CAAAGCTCAT GGCCACCAAG ACCGAGGCCA CCAAACCTGT GGCTGCCAGG 
CCCGAGGCGG CAAAGACAGC CACAGCCAGA CCTGCAGCAG CCGCGAAGCC TGCAGCAGCC 
AAACCTGCCC CTGCCAGACC CCCCACTGCT GCCAGATCCA TGGCCACGAG GTCCGAGGCC 
CCCAGGCCCC AGGCAGTCAA ACTGGCTGCC AGCAGGCCCG GCGCTGCTAA GCCCGTGGTG 
AAGGCACCTC GCGAGATCCA TGTGTCTGAG GTCACAGAGA ACAGCGCCAA GCTGCACTGG 
GAGAGGCCCG AGCCCCCCAG CCCTTACTTA TACAACCTCA CCGTCACCTC GGCCCACGAT 
CAGGCCGTGG TTCTGAAGCA GAACCTCACT GTCACGGACC GCGTCATCGG GGGCCTGCTC 
CCCGGGCAGA CTTACCACGT TACGGTGACC TGCTCCCTGA GGTCTCAGGT CAGAGCCATC 
TACCAAGGCA GCTTCAGCAC GAAGAAAATT CAGCCTCCAC CTCCACAAAC AGAGCGATCA 
GCCTCTAGTG CAACCATCAA CCTAGTGGTG AGCGCAGACC GGTTGGCTGG GAGTAAAGCA 
GATATATGTA AGTTGCCAAA GGACGGCGGA ACTTGCAGAG AGTTTGTATT AAAGTGGTAC 
TATGATTCCG TAACTGAAAA CTGCGCCCGA TTCTGGTATG GAGGCTGCGG TGGAAACGAA 
AACAGATTTA ATTCGCAGGA TGAATGTGAA AAGGTTTGCC CTCCTGTGCC CATCAAGCAG 
CCCGGAGTTA TCGCCGCGAT GGGGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_005197963.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005198020.1 Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X1, mRNA.

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CloneID	OBa03695
Clone ID Related Accession (Same CDS sequence)	XM_005197963.3 , XM_005204978.3
Accession Version	XM_005197963.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644673.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197963.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA 
TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT 
CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC 
CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA 
GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA 
TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA 
GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT 
GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG 
AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG 
GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT 
GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT 
CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG 
GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC 
AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT 
GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA 
GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC 
CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC 
GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC 
AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC 
AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT 
ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC 
TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA 
GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC 
GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC 
CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC 
GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG 
GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC 
GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGTTCGCG TAAACAAAAG GGATATCATC 
TTTCTTTTGG ATGGATCGTC CAACGTCGGA GAGACCAATT TCCCTTATGT GCGGGACTTT 
GTCATGAACC TAGTTAACAG CCTTGATGTT GGAAGCGACC ACATTCGGGT CGGTCTAGTG 
CAATTTAGTG ACACTCCGGT GACGGAGTTC TCCCTAAACA CCTACCCGAC CAAGTCAGAG 
TTGCTAGCTC ACCTGAGGCA GATGCAGCTC CAGGGGGGGT CGGTCCTGAA CACGGGCGCG 
GCCCTGAGCT ATGTGCATGC CAACCACTTC ACCGAAGCTG GCGGCAGCAG GATCCAGGAC 
CACGTGCCGC AGCTGCTACT CCTGCTCACG GCCGGGCAGT CTGAGGACTC CTATTTGCAA 
GCCGCCAACG CCCTGGCGCG CGCGGGCATC CTGACCTTTT GTGTGGGCAC TAGCCAGGCC 
GATAGGGCGG AGCTTGAGGA GATTGCTTTT AACCCGGGCC TGGTGTATCT CATGGATGAT 
TTCAGCTCCT TGCCAGCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC TTATGTTAGC 
GGAGGTGTGG AGGAGGTGCC TCTTGCCCAG CCAGAGAGCA AGCGAGACAT CCTGTTCCTT 
TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG 
GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT 
GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT 
CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT 
TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT 
CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC 
AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT 
GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG 
ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG 
GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG 
AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA 
CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC 
CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG 
GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG 
GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC 
GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG 
CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC 
CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC 
TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC 
CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC 
CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC 
CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG 
GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG 
CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG 
AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC 
CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG 
TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC 
TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG 
CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC 
AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC 
GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG 
ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC 
AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT 
GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA 
TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC 
CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG 
GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA 
GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC 
CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA 
CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG 
TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT 
GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC 
CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG 
ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG 
GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG 
AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG 
GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC 
GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC 
GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT 
GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG 
GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG 
GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG 
CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT 
GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC 
CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG 
ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT 
TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC 
CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG 
TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT 
GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA 
GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA 
GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT 
CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA 
GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA 
GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC 
AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA 
CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC 
CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC 
CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA 
GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG 
ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA 
GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG 
GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT 
GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA 
AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC 
CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA 
GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG 
AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG 
GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG 
AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG 
GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC 
GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC 
TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT 
GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG 
GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG 
TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG 
GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC 
CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC 
TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT 
TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC 
ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT 
GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG 
CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC 
CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC 
ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG 
ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG 
GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG 
GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG 
ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC 
AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA 
GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC 
GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC 
AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT 
GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC 
ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT 
GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC 
ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT 
GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA 
GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC 
GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC 
GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG 
CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC 
CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC 
CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA 
GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG 
CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT 
AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG 
TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA 
AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC 
AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005198020.1
CDS	348..10043
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPDVKNGAAADIIFLVDSSWSIGKEHFQLVREFLYDVIESLAVGDNDFRFALVQFNGNPHTEFLFNTYRSKQEVLSHVSNMSYIGGSNQTGKGLAYVMQNHLTEAAGSRASDGVPQVIVVLTHGHSEDGLALPSAELKSADVNVFAIGVEDADEAALKEIASEPLNMHVFNLENYTSLHDIVGNLVACVRSSMAPERAGGTEIPKDITAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLERLSIGTQQIRVGVVQYSDEPRTMFSLNSYSTKAQVLDAVKALGFIGGELANVGLALDFVVENHFTRAGGSRAEEGVPQVLVLISAGPSSDEIRDGVIALKQASVFSFGLGAQAASKAELQHIATNDNLVFTVPEFRSLGDVQEQLLPYIVGVAQRHIVLQPPTIVTQVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEVRVNKRDIIFLLDGSSNVGETNFPYVRDFVMNLVNSLDVGSDHIRVGLVQFSDTPVTEFSLNTYPTKSELLAHLRQMQLQGGSVLNTGAALSYVHANHFTEAGGSRIQDHVPQLLLLLTAGQSEDSYLQAANALARAGILTFCVGTSQADRAELEEIAFNPGLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_005197963.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197963.3

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA 
TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT 
CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC 
CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA 
GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA 
TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA 
GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT 
GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG 
AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG 
GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT 
GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT 
CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG 
GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC 
AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT 
GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA 
GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC 
CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC 
GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC 
AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC 
AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT 
ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC 
TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA 
GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC 
GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC 
CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC 
GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG 
GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC 
GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGTTCGCG TAAACAAAAG GGATATCATC 
TTTCTTTTGG ATGGATCGTC CAACGTCGGA GAGACCAATT TCCCTTATGT GCGGGACTTT 
GTCATGAACC TAGTTAACAG CCTTGATGTT GGAAGCGACC ACATTCGGGT CGGTCTAGTG 
CAATTTAGTG ACACTCCGGT GACGGAGTTC TCCCTAAACA CCTACCCGAC CAAGTCAGAG 
TTGCTAGCTC ACCTGAGGCA GATGCAGCTC CAGGGGGGGT CGGTCCTGAA CACGGGCGCG 
GCCCTGAGCT ATGTGCATGC CAACCACTTC ACCGAAGCTG GCGGCAGCAG GATCCAGGAC 
CACGTGCCGC AGCTGCTACT CCTGCTCACG GCCGGGCAGT CTGAGGACTC CTATTTGCAA 
GCCGCCAACG CCCTGGCGCG CGCGGGCATC CTGACCTTTT GTGTGGGCAC TAGCCAGGCC 
GATAGGGCGG AGCTTGAGGA GATTGCTTTT AACCCGGGCC TGGTGTATCT CATGGATGAT 
TTCAGCTCCT TGCCAGCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC TTATGTTAGC 
GGAGGTGTGG AGGAGGTGCC TCTTGCCCAG CCAGAGAGCA AGCGAGACAT CCTGTTCCTT 
TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG 
GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT 
GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT 
CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT 
TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT 
CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC 
AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT 
GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG 
ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG 
GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG 
AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA 
CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC 
CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG 
GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG 
GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC 
GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG 
CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC 
CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC 
TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC 
CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC 
CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC 
CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG 
GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG 
CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG 
AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC 
CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG 
TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC 
TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG 
CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC 
AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC 
GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG 
ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC 
AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT 
GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA 
TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC 
CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG 
GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA 
GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC 
CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA 
CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG 
TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT 
GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC 
CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG 
ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG 
GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG 
AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG 
GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC 
GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC 
GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT 
GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG 
GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG 
GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG 
CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT 
GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC 
CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG 
ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT 
TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC 
CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG 
TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT 
GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA 
GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA 
GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT 
CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA 
GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA 
GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC 
AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA 
CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC 
CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC 
CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA 
GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG 
ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA 
GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG 
GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT 
GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA 
AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC 
CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA 
GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG 
AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG 
GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG 
AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG 
GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC 
GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC 
TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT 
GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG 
GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG 
TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG 
GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC 
CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC 
TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT 
TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC 
ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT 
GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG 
CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC 
CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC 
ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG 
ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG 
GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG 
GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG 
ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC 
AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA 
GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC 
GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC 
AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT 
GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC 
ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT 
GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC 
ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT 
GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA 
GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC 
GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC 
GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG 
CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC 
CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC 
CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA 
GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG 
CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT 
AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG 
TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA 
AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC 
AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_005204978.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205035.1 Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X1, mRNA.

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CloneID	OBa03695
Clone ID Related Accession (Same CDS sequence)	XM_005197963.3 , XM_005204978.3
Accession Version	XM_005204978.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204978.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA 
TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT 
CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC 
CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA 
GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA 
TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA 
GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT 
GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG 
AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG 
GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT 
GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT 
CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG 
GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC 
AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT 
GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA 
GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC 
CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC 
GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC 
AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC 
AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT 
ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC 
TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA 
GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC 
GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC 
CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC 
GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG 
GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC 
GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGTTCGCG TAAACAAAAG GGATATCATC 
TTTCTTTTGG ATGGATCGTC CAACGTCGGA GAGACCAATT TCCCTTATGT GCGGGACTTT 
GTCATGAACC TAGTTAACAG CCTTGATGTT GGAAGCGACC ACATTCGGGT CGGTCTAGTG 
CAATTTAGTG ACACTCCGGT GACGGAGTTC TCCCTAAACA CCTACCCGAC CAAGTCAGAG 
TTGCTAGCTC ACCTGAGGCA GATGCAGCTC CAGGGGGGGT CGGTCCTGAA CACGGGCGCG 
GCCCTGAGCT ATGTGCATGC CAACCACTTC ACCGAAGCTG GCGGCAGCAG GATCCAGGAC 
CACGTGCCGC AGCTGCTACT CCTGCTCACG GCCGGGCAGT CTGAGGACTC CTATTTGCAA 
GCCGCCAACG CCCTGGCGCG CGCGGGCATC CTGACCTTTT GTGTGGGCAC TAGCCAGGCC 
GATAGGGCGG AGCTTGAGGA GATTGCTTTT AACCCGGGCC TGGTGTATCT CATGGATGAT 
TTCAGCTCCT TGCCAGCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC TTATGTTAGC 
GGAGGTGTGG AGGAGGTGCC TCTTGCCCAG CCAGAGAGCA AGCGAGACAT CCTGTTCCTT 
TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG 
GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT 
GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT 
CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT 
TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT 
CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC 
AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT 
GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG 
ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG 
GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG 
AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA 
CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC 
CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG 
GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG 
GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC 
GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG 
CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC 
CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC 
TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC 
CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC 
CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC 
CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG 
GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG 
CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG 
AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC 
CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG 
TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC 
TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG 
CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC 
AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC 
GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG 
ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC 
AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT 
GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA 
TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC 
CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG 
GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA 
GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC 
CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA 
CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG 
TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT 
GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC 
CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG 
ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG 
GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG 
AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG 
GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC 
GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC 
GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT 
GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG 
GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG 
GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG 
CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT 
GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC 
CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG 
ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT 
TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC 
CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG 
TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT 
GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA 
GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA 
GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT 
CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA 
GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA 
GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC 
AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA 
CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC 
CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC 
CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA 
GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG 
ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA 
GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG 
GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT 
GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA 
AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC 
CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA 
GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG 
AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG 
GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG 
AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG 
GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC 
GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC 
TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT 
GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG 
GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG 
TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG 
GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC 
CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC 
TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT 
TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC 
ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT 
GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG 
CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC 
CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC 
ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG 
ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG 
GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG 
GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG 
ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC 
AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA 
GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC 
GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC 
AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT 
GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC 
ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT 
GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC 
ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT 
GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA 
GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC 
GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC 
GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG 
CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC 
CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC 
CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA 
GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG 
CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT 
AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG 
TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA 
AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC 
AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205035.1
CDS	348..10043
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPDVKNGAAADIIFLVDSSWSIGKEHFQLVREFLYDVIESLAVGDNDFRFALVQFNGNPHTEFLFNTYRSKQEVLSHVSNMSYIGGSNQTGKGLAYVMQNHLTEAAGSRASDGVPQVIVVLTHGHSEDGLALPSAELKSADVNVFAIGVEDADEAALKEIASEPLNMHVFNLENYTSLHDIVGNLVACVRSSMAPERAGGTEIPKDITAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLERLSIGTQQIRVGVVQYSDEPRTMFSLNSYSTKAQVLDAVKALGFIGGELANVGLALDFVVENHFTRAGGSRAEEGVPQVLVLISAGPSSDEIRDGVIALKQASVFSFGLGAQAASKAELQHIATNDNLVFTVPEFRSLGDVQEQLLPYIVGVAQRHIVLQPPTIVTQVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEVRVNKRDIIFLLDGSSNVGETNFPYVRDFVMNLVNSLDVGSDHIRVGLVQFSDTPVTEFSLNTYPTKSELLAHLRQMQLQGGSVLNTGAALSYVHANHFTEAGGSRIQDHVPQLLLLLTAGQSEDSYLQAANALARAGILTFCVGTSQADRAELEEIAFNPGLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_005204978.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204978.3

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA 
TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT 
CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC 
CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA 
GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA 
TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA 
GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT 
GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG 
AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG 
GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT 
GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT 
CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG 
GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC 
AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT 
GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA 
GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC 
CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC 
GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC 
AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC 
AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT 
ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC 
TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA 
GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC 
GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC 
CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC 
GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG 
GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC 
GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGTTCGCG TAAACAAAAG GGATATCATC 
TTTCTTTTGG ATGGATCGTC CAACGTCGGA GAGACCAATT TCCCTTATGT GCGGGACTTT 
GTCATGAACC TAGTTAACAG CCTTGATGTT GGAAGCGACC ACATTCGGGT CGGTCTAGTG 
CAATTTAGTG ACACTCCGGT GACGGAGTTC TCCCTAAACA CCTACCCGAC CAAGTCAGAG 
TTGCTAGCTC ACCTGAGGCA GATGCAGCTC CAGGGGGGGT CGGTCCTGAA CACGGGCGCG 
GCCCTGAGCT ATGTGCATGC CAACCACTTC ACCGAAGCTG GCGGCAGCAG GATCCAGGAC 
CACGTGCCGC AGCTGCTACT CCTGCTCACG GCCGGGCAGT CTGAGGACTC CTATTTGCAA 
GCCGCCAACG CCCTGGCGCG CGCGGGCATC CTGACCTTTT GTGTGGGCAC TAGCCAGGCC 
GATAGGGCGG AGCTTGAGGA GATTGCTTTT AACCCGGGCC TGGTGTATCT CATGGATGAT 
TTCAGCTCCT TGCCAGCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC TTATGTTAGC 
GGAGGTGTGG AGGAGGTGCC TCTTGCCCAG CCAGAGAGCA AGCGAGACAT CCTGTTCCTT 
TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG 
GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT 
GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT 
CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT 
TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT 
CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC 
AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT 
GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG 
ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG 
GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG 
AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA 
CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC 
CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG 
GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG 
GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC 
GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG 
CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC 
CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC 
TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC 
CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC 
CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC 
CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG 
GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG 
CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG 
AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC 
CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG 
TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC 
TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG 
CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC 
AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC 
GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG 
ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC 
AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT 
GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA 
TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC 
CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG 
GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA 
GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC 
CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA 
CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG 
TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT 
GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC 
CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG 
ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG 
GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG 
AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG 
GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC 
GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC 
GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT 
GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG 
GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG 
GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG 
CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT 
GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC 
CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG 
ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT 
TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC 
CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG 
TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT 
GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA 
GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA 
GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT 
CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA 
GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA 
GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC 
AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA 
CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC 
CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC 
CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA 
GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG 
ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA 
GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG 
GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT 
GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA 
AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC 
CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA 
GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG 
AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG 
GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG 
AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG 
GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC 
GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC 
TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT 
GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG 
GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG 
TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG 
GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC 
CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC 
TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT 
TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC 
ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT 
GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG 
CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC 
CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC 
ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG 
ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG 
GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG 
GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG 
ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC 
AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA 
GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC 
GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC 
AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT 
GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC 
ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT 
GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC 
ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT 
GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA 
GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC 
GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC 
GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG 
CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC 
CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC 
CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA 
GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG 
CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT 
AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG 
TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA 
AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC 
AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_005197964.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005198021.1 Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X2, mRNA.

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CloneID	OBa03697
Clone ID Related Accession (Same CDS sequence)	XM_005197964.3 , XM_005204979.3
Accession Version	XM_005197964.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644673.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197964.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA 
TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT 
CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC 
CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA 
GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA 
TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA 
GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT 
GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG 
AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG 
GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT 
GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT 
CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG 
GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC 
AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT 
GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA 
GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC 
CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC 
GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC 
AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC 
AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT 
ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC 
TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA 
GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC 
GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC 
CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC 
GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG 
GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC 
GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGAGAGCA AGCGAGACAT CCTGTTCCTT 
TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG 
GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT 
GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT 
CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT 
TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT 
CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC 
AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT 
GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG 
ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG 
GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG 
AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA 
CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC 
CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG 
GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG 
GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC 
GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG 
CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC 
CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC 
TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC 
CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC 
CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC 
CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG 
GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG 
CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG 
AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC 
CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG 
TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC 
TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG 
CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC 
AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC 
GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG 
ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC 
AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT 
GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA 
TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC 
CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG 
GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA 
GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC 
CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA 
CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG 
TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT 
GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC 
CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG 
ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG 
GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG 
AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG 
GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC 
GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC 
GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT 
GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG 
GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG 
GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG 
CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT 
GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC 
CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG 
ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT 
TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC 
CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG 
TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT 
GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA 
GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA 
GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT 
CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA 
GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA 
GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC 
AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA 
CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC 
CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC 
CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA 
GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG 
ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA 
GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG 
GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT 
GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA 
AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC 
CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA 
GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG 
AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG 
GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG 
AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG 
GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC 
GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC 
TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT 
GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG 
GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG 
TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG 
GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC 
CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC 
TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT 
TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC 
ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT 
GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG 
CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC 
CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC 
ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG 
ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG 
GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG 
GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG 
ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC 
AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA 
GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC 
GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC 
AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT 
GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC 
ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT 
GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC 
ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT 
GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA 
GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC 
GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC 
GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG 
CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC 
CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC 
CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA 
GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG 
CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT 
AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG 
TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA 
AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC 
AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005198021.1
CDS	346..9441
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPDVKNGAAADIIFLVDSSWSIGKEHFQLVREFLYDVIESLAVGDNDFRFALVQFNGNPHTEFLFNTYRSKQEVLSHVSNMSYIGGSNQTGKGLAYVMQNHLTEAAGSRASDGVPQVIVVLTHGHSEDGLALPSAELKSADVNVFAIGVEDADEAALKEIASEPLNMHVFNLENYTSLHDIVGNLVACVRSSMAPERAGGTEIPKDITAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLERLSIGTQQIRVGVVQYSDEPRTMFSLNSYSTKAQVLDAVKALGFIGGELANVGLALDFVVENHFTRAGGSRAEEGVPQVLVLISAGPSSDEIRDGVIALKQASVFSFGLGAQAASKAELQHIATNDNLVFTVPEFRSLGDVQEQLLPYIVGVAQRHIVLQPPTIVTQVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_005197964.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197964.3

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA 
TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT 
CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC 
CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA 
GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA 
TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA 
GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT 
GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG 
AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG 
GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT 
GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT 
CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG 
GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC 
AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT 
GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA 
GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC 
CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC 
GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC 
AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC 
AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT 
ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC 
TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA 
GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC 
GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC 
CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC 
GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG 
GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC 
GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGAGAGCA AGCGAGACAT CCTGTTCCTT 
TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG 
GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT 
GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT 
CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT 
TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT 
CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC 
AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT 
GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG 
ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG 
GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG 
AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA 
CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC 
CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG 
GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG 
GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC 
GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG 
CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC 
CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC 
TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC 
CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC 
CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC 
CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG 
GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG 
CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG 
AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC 
CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG 
TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC 
TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG 
CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC 
AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC 
GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG 
ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC 
AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT 
GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA 
TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC 
CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG 
GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA 
GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC 
CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA 
CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG 
TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT 
GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC 
CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG 
ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG 
GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG 
AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG 
GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC 
GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC 
GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT 
GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG 
GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG 
GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG 
CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT 
GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC 
CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG 
ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT 
TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC 
CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG 
TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT 
GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA 
GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA 
GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT 
CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA 
GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA 
GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC 
AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA 
CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC 
CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC 
CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA 
GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG 
ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA 
GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG 
GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT 
GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA 
AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC 
CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA 
GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG 
AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG 
GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG 
AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG 
GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC 
GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC 
TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT 
GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG 
GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG 
TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG 
GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC 
CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC 
TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT 
TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC 
ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT 
GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG 
CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC 
CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC 
ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG 
ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG 
GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG 
GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG 
ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC 
AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA 
GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC 
GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC 
AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT 
GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC 
ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT 
GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC 
ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT 
GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA 
GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC 
GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC 
GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG 
CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC 
CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC 
CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA 
GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG 
CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT 
AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG 
TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA 
AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC 
AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_005204979.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205036.1 Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X2, mRNA.

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CloneID	OBa03697
Clone ID Related Accession (Same CDS sequence)	XM_005197964.3 , XM_005204979.3
Accession Version	XM_005204979.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204979.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA 
TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT 
CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC 
CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA 
GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA 
TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA 
GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT 
GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG 
AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG 
GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT 
GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT 
CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG 
GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC 
AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT 
GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA 
GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC 
CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC 
GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC 
AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC 
AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT 
ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC 
TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA 
GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC 
GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC 
CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC 
GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG 
GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC 
GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGAGAGCA AGCGAGACAT CCTGTTCCTT 
TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG 
GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT 
GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT 
CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT 
TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT 
CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC 
AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT 
GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG 
ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG 
GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG 
AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA 
CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC 
CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG 
GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG 
GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC 
GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG 
CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC 
CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC 
TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC 
CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC 
CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC 
CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG 
GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG 
CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG 
AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC 
CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG 
TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC 
TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG 
CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC 
AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC 
GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG 
ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC 
AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT 
GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA 
TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC 
CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG 
GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA 
GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC 
CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA 
CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG 
TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT 
GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC 
CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG 
ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG 
GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG 
AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG 
GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC 
GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC 
GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT 
GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG 
GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG 
GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG 
CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT 
GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC 
CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG 
ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT 
TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC 
CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG 
TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT 
GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA 
GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA 
GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT 
CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA 
GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA 
GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC 
AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA 
CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC 
CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC 
CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA 
GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG 
ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA 
GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG 
GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT 
GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA 
AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC 
CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA 
GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG 
AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG 
GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG 
AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG 
GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC 
GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC 
TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT 
GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG 
GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG 
TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG 
GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC 
CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC 
TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT 
TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC 
ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT 
GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG 
CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC 
CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC 
ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG 
ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG 
GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG 
GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG 
ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC 
AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA 
GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC 
GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC 
AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT 
GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC 
ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT 
GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC 
ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT 
GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA 
GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC 
GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC 
GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG 
CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC 
CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC 
CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA 
GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG 
CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT 
AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG 
TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA 
AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC 
AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205036.1
CDS	346..9441
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPDVKNGAAADIIFLVDSSWSIGKEHFQLVREFLYDVIESLAVGDNDFRFALVQFNGNPHTEFLFNTYRSKQEVLSHVSNMSYIGGSNQTGKGLAYVMQNHLTEAAGSRASDGVPQVIVVLTHGHSEDGLALPSAELKSADVNVFAIGVEDADEAALKEIASEPLNMHVFNLENYTSLHDIVGNLVACVRSSMAPERAGGTEIPKDITAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLERLSIGTQQIRVGVVQYSDEPRTMFSLNSYSTKAQVLDAVKALGFIGGELANVGLALDFVVENHFTRAGGSRAEEGVPQVLVLISAGPSSDEIRDGVIALKQASVFSFGLGAQAASKAELQHIATNDNLVFTVPEFRSLGDVQEQLLPYIVGVAQRHIVLQPPTIVTQVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_005204979.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204979.3

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA 
TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT 
CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC 
CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA 
GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA 
TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA 
GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT 
GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG 
AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG 
GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT 
GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT 
CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG 
GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC 
AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT 
GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA 
GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC 
CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC 
GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC 
AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC 
AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT 
ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC 
TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA 
GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC 
GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC 
CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC 
GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG 
GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC 
GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGAGAGCA AGCGAGACAT CCTGTTCCTT 
TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG 
GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT 
GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT 
CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT 
TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT 
CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC 
AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT 
GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG 
ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG 
GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG 
AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA 
CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC 
CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG 
GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG 
GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC 
GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG 
CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC 
CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC 
TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC 
CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC 
CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC 
CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG 
GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG 
CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG 
AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC 
CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG 
TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC 
TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG 
CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC 
AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC 
GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG 
ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC 
AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT 
GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA 
TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC 
CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG 
GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA 
GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC 
CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA 
CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG 
TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT 
GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC 
CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG 
ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG 
GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG 
AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG 
GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC 
GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC 
GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT 
GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG 
GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG 
GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG 
CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT 
GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC 
CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG 
ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT 
TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC 
CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG 
TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT 
GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA 
GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA 
GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT 
CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA 
GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA 
GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC 
AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA 
CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC 
CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC 
CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA 
GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG 
ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA 
GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG 
GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT 
GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA 
AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC 
CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA 
GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG 
AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG 
GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG 
AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG 
GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC 
GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC 
TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT 
GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG 
GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG 
TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG 
GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC 
CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC 
TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT 
TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC 
ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT 
GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG 
CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC 
CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC 
ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG 
ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG 
GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG 
GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG 
ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC 
AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA 
GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC 
GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC 
AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT 
GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC 
ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT 
GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC 
ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT 
GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA 
GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC 
GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC 
GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG 
CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC 
CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC 
CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA 
GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG 
CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT 
AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG 
TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA 
AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC 
AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_003582038.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003582086.2 Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X3, mRNA.

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CloneID	OBa03698
Clone ID Related Accession (Same CDS sequence)	XM_003582038.4 , XM_002686571.5
Accession Version	XM_003582038.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644673.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582038.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGCA CAAGACTCTG CTGACATTAT TTTCCTTATT 
GATGGATCAA ACAACACCGG AAGTGTCAAT TTCGCAGTCA TTCTCGACTT CCTTGTAAAT 
CTCCTTGAGA GACTGTCAAT TGGAACTCAG CAGATCCGAG TGGGGGTGGT GCAGTATAGC 
GATGAGCCCA GAACCATGTT CTCCTTGAAC AGCTACTCCA CCAAGGCTCA GGTTCTGGAT 
GCAGTGAAGG CCCTGGGGTT CATTGGTGGA GAGCTGGCCA ATGTTGGCCT TGCCCTGGAT 
TTTGTGGTGG AGAATCACTT CACCCGGGCG GGGGGCAGCC GAGCAGAGGA AGGGGTTCCC 
CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATCCGAGA CGGGGTCATA 
GCACTGAAGC AGGCTAGCGT TTTCTCGTTT GGCCTCGGAG CCCAGGCCGC CTCCAAGGCA 
GAGCTTCAGC ACATAGCTAC CAATGACAAC CTGGTGTTTA CTGTCCCGGA ATTCCGTAGC 
CTTGGGGACG TCCAGGAACA ATTACTGCCG TACATTGTTG GCGTGGCCCA AAGGCACATT 
GTCTTGCAAC CGCCAACCAT TGTCACCCAA GTCATTGAAG TCAACAAGAG GGACATAGTT 
TTCCTGGTGG ATGGCTCTTC CAAACTGGGA CTGACCAACT TCAATGCAAT CCGCGACTTC 
ATTGCCAAGG TCATCCAGAG GCTGGAGATC AGACAGGATC TTATCCAGGT GGCAGTGGCT 
CAGTATGCAG ACACAGTGAG GCCAGAGTTT TATTTCAATA CCTACCCCAG CAAGAGGGAG 
GTCATAAACG CCGTGCGGAA AATGAAGGCT TTGGATGGCT CAGCCTTGTA CACGGGCTCC 
GCTCTGGATT TTGTTCGGAA CAACCTGTTC ACGGAGGCGG CCGGCTACCG GGCTGCCGAG 
GGGGTCCCTA AGCTTCTGGT GCTGGTTACA GGCGGTAAGT CCCTAGATGC GGTCAGCCAG 
CCCGCCCAGG AGCTGAAGAG GAGCGGCATC CTGGCCTTCG CCGTCGGGAA CAAGGTGGCT 
GACCAGGCTG AACTGGAAGA GATCGCTTTC GATTCCTCCC TGGTGTTCAC TGCCACCGAG 
TTCCGACCCG CCCCTTTGCA GGGCGTGCTG CCCGGCTTGC TCGGGCCTCT CAGGACCCTC 
ACTGGAACCA CTGAAGTTCG CGTAAACAAA AGGGATATCA TCTTTCTTTT GGATGGATCG 
TCCAACGTCG GAGAGACCAA TTTCCCTTAT GTGCGGGACT TTGTCATGAA CCTAGTTAAC 
AGCCTTGATG TTGGAAGCGA CCACATTCGG GTCGGTCTAG TGCAATTTAG TGACACTCCG 
GTGACGGAGT TCTCCCTAAA CACCTACCCG ACCAAGTCAG AGTTGCTAGC TCACCTGAGG 
CAGATGCAGC TCCAGGGGGG GTCGGTCCTG AACACGGGCG CGGCCCTGAG CTATGTGCAT 
GCCAACCACT TCACCGAAGC TGGCGGCAGC AGGATCCAGG ACCACGTGCC GCAGCTGCTA 
CTCCTGCTCA CGGCCGGGCA GTCTGAGGAC TCCTATTTGC AAGCCGCCAA CGCCCTGGCG 
CGCGCGGGCA TCCTGACCTT TTGTGTGGGC ACTAGCCAGG CCGATAGGGC GGAGCTTGAG 
GAGATTGCTT TTAACCCGGG CCTGGTGTAT CTCATGGATG ATTTCAGCTC CTTGCCAGCT 
TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACTTATGTTA GCGGAGGTGT GGAGGAGGTG 
CCTCTTGCCC AGCCAGAGAG CAAGCGAGAC ATCCTGTTCC TTTTCGACGG CTCAGCCAAC 
CTCATGGGCC AGTTCACTGC CGCCCGTGAC TTTCTCTACA AGGTCATCGA CGAGCTGGAT 
GTGAAACCTG AGGGGACCCG GGTTGCAGTG GCTCAGTTCA GTGATGATGT CAAGGTGGAG 
TCCCGTTTTG ACCAGCACCA GAATAAGCCT GAGATCCTGA ATCTCGTGAA GAGGATGAAG 
CTCAAGACAG GCAAAGCCCT CAACCTGGGC TACGCCTTGG ATTACGCACA GAGGTACATC 
TTTGTGAAGT CCGCTGGAAG CCGGATTGAG GATGGAGTGC TTCAGTTCCT GGTGCTGCTG 
GTGGCGGGAA AGTCATCGGA CCGGGTGGAT ACACCCGCAC TCAACCTGAA GCAGAGCGGG 
GTGGTGCCCT TCATCCTCCA GGCGAAAAAC GCGGACCCTG CTGAGTTGGA GCTGATCGTA 
CCATCCCCAG CCTTTATTTT GGTTGCGGAG TCCCTTCCTA AGATTGGAGA CCTGCAACCA 
CAGATTGTGA ACCTCTTAAA ATCAGTGCAA AATGGGGCAC CGGCACCAGT TTCAGTTGAA 
AAGGATGTGG TGTTCCTGAT TGATGGCTCC GAGGGTGTCA GGAGCGGCTT CCCACTGCTG 
AAGGAGTTCG TCCAGAGAGT GGTGGAGAGC CTGGACGTGG GACCAGACCG GGTGCGTGTG 
GCCGTGGTGC AGTACAGCGA CCGGACCAGA CCTGAGTTCT ACCTGAATTC CTACATGGAC 
CAGCAGAGTG TGGTCGGAGC CATCCGTGGG CTGACCCTGC TGGGCGGGCC GGCCCCCAAC 
ACAGGGGCTG CCCTGGAGTT CGTCCTGAGG AACATCCTGG TGGGCTCTGC TGGGAGCCGC 
ATAGCAGAAG GTGTCCCCCA GCTCCTGATT GTCCTCACGG CCGACAGGTC TGGGGATGAC 
GTGAGGGGCC CCTCTGTGGT CCTGAGGAGG GGAGGGGCCG TGCCCATCGG CATCGGCATT 
GGGAACGCTG ACATCACCGA GATGCAGACC CTCTCCTTCG TCCCGGACTT CGCCGTGGTC 
ATCCCCACCT TCCGGCAGCT GGGGACCATC CAACAGGTCA TCTCGGAGAG AGTGACCCAG 
CTCAGCCGCG AGGAACTGAG CAGGCTGCAG GCGTCTGTCA CCCCCTTGAC GACTCCGGTG 
GTCAGCAGCA AGAGGGACGT GGTCTTTCTC ATCGATGGGT CCCAGAGTGC TGGCCCCGAG 
TTCCAGTACA TCCGAACGCT CATCGAGAGG CTGGTGGACT ACCTGGATGT GGGCTTCGAC 
ACGACCCGGG TGGCGGTCAT CCAGTTCAGC GATGACCCCA GGGTGGAGTT CCTGTTGAAC 
GTCCACTCCA GCAAGGACGA GGTGCAGAAC GCCGTGCGGC GGCTGCGGCC CAAAGGTGGG 
AGGCAGGTCA ACGTTGGGGG TGCCCTGGAG TACGTGGCCA GGAACATCTT CAAGAGGCCC 
CTGGGGAGCC GGATTGAGGA GGGTGTCCCT CAGTTCCTGG TCCTCATTTC CTCCGGGAAG 
TCGGATGATG AGGTGGAGGA CTCGGCCATA GAGCTCAAGC AGTTTGGCGT GGCGCCACTC 
ACGATCGCCA GGAACGTGGA CCAGGAAGAG CTGGTGAAGA TCTCGCTGAG CCCCGAGTAC 
GTGTTCTCAG TGAACACCTT CCGGGAGCTC CCCAGCCTGG AGCAGAAACT GCTGACACCG 
CTCACCACCC TGACTGCCGG GCAGATACAG CAGCTCCTGG CCAGCACGCG CTACCCGCCT 
CCAGCTGTGG AGAGCGATGC TGCGGACATT GTCTTCCTCA TCGACAGCTC TGATGGCGTG 
AAGCCGGATG GCATCGCACA CATCCGGGAC TTTGTTATCA GGATCGTGCG GAGACTCAAC 
GTCGGTCCCA ACAAAGTGAG AATTGGGGTC CTGCAGTTCA GCAACGACGT CTTCCCAGAA 
TTTCAACTAA AGACCTATAA ATCCCAGGCC AGCGTGCTGG ATGCCATACG TCGCCTGAGG 
TTCAAAGGGG GGTCTCCTCT CAACACTGGC AAGGCTCTGG AATTCGTGGC GAGGAATTAC 
TTTGTCAAGT CTGCCGGGAG CCGGATAGAA GATGGGGTGC CCCAACACCT GGTTCTATTT 
CTGGGTGGAA AATCCCAGGA TGATATTTCC AGGTATTCCC AGGTGATAAA ATCTGCAGGA 
ATTGCGAGCT TAGGGGTTGG AGACCGAAAT ATTGACAGAA CAGAGCTGCA GACCATCACC 
AGCGATCCCA GGCTTGTCTT CACGGTGCGG GAGTTCAGAG ACCTCCCCAG CATAGAAGAA 
AGGATGGTGA ATTCCTTTGG GTCCTCCGGG GTCACCCCTG CACCTCCGGG AGTGGACACA 
CCCTCTCCTT CAAGGCCAGA GAAGAAGAAA GCCGACATTG TGTTCCTGCT GGATGGTTCC 
ATCAATTTCC GGAGAGACAG TTTCCAGGAA GTGCTCCGTT TTGTGTCAGA AATTGTGGAC 
ACGGTTTATG AAGGGGGCGA CTCCATCCAG GTGGGGCTGG TCCAATACAA CTCTGACCCC 
ACCGACGAGT TCTTCCTGAA GGACTTCCCC ACCAAGCAGC AGATTATCGA CGCCATAAAC 
AAGGTGGTCT ACAAAGGGGG GAGGCACGCG AACACCAAGG TGGGCCTGGA GCACCTGCGG 
CGGAACCACT TCGTGCCCGA GGCGGGCAGC CGCCTGGACC AGAGGGTCCC GCAGATCGCC 
TTTGTGATCA CGGGGGGCAA GTCGGTGGAG GACGCTCAGG AGGCAAGCAT GGCGCTCACG 
CAGAGAGGCG TCAAGGTGTT TGCGGTGGGC GTAAGGAACA TCGACTCGGA GGAGGTTGGG 
AAGATAGCGT CCAACAGCGC CACGGCCTTC CGGGTGGGGA ACGTCCAGGA GCTGTCGGAA 
CTGAGCGAGC AGGTTTTGGA AACTCTGCAC GATGCAATGC ATGAGACCTT ATGTCCAGGC 
GTGACTGACG TTTCCAAAGC CTGTAACTTG GATGTGATTC TGGGCTTCGA TGGTTCAGGA 
GATCAGAATG TCTTTGTGGC TCAGAAGGGC CTCGAGCCCA AGGTGGACAC CATCCTGAGG 
AGAATCAGTC AGATGCAGAA GATCAGCTGC AGCGGCAGCC AGCTGCCCAC CGTGCGGGTG 
TCTGTGGTGG CCCTCACGCC CTCGGGCCCC GTTGAGGCCT TTGACTTTGC TGAGTACCAG 
CCGGAGCTGT TTGAGAAGTT CCAGAACATG CGTGCCCAGC ACCCCTACGT CCTCACTGCG 
GACACACTGA AGCTCTACCA GAACAAGTTC CGGCAGGCCT CGATGGACAA CGTGAAGGTG 
GTCATTCATT TTACCGATGG AGTGGATGGG GATCTGGCTG ATTTACAAAG AGCATCAGAG 
GAACTCCGAC AAGAAGGTGT CCGAGCTCTG ATCTTGGTGG GCCTCGAACG CGTCGCCAAC 
TTGGAGCAGC TCATGCAGCT GGAGTTCGGG CGAGGGTTCA CGTACAACCG GCCCCTGAGG 
CTGAACTTGC TGGACCTGGA CTACGAACTA GCCGAGCAGC TTGACAACAT TGCTGAGAAA 
GCCTGCTGTG GGATTCCCTG CAAATGCTCT GGACAAAGAG GAGACCGAGG GCCCATAGGC 
AGCATCGGGC CAAAGGGCAT TCCTGGAGAA GATGGCTACC GAGGTTATCC TGGTGACGAA 
GGTGGACCTG GTGAACGCGG TCCTCCTGGC GTGAACGGCA CTCAAGGTTT CCAGGGCTGC 
CCCGGGCAGA GAGGAGTCAA GGGCTCTCGT GGATTCCCGG GAGAGAAGGG TGAATTAGGA 
GAAATTGGAC TGGATGGTCT TGATGGTGAA GATGGCGACA AAGGGCTGCC CGGTACTTCT 
GGAGAGAAAG GGAGTCCAGG AAGGAGGGGC GACAAAGGAC CCAAAGGCGA CAAAGGGGAG 
AGAGGCGATG TCGGGATCAG AGGCGACCCG GGCAATTCAG GACAGGACAG CCAGCAGAGA 
GGAGCCAAAG GAGAAACCGG AGACATCGGC CCCGTGGGTC TCCCTGGGAG AGACGGGGTG 
TCTGGAGGGC CTGGAGAACC CGGGAAGAGC GGTGGCTTTG GCCGAAGGGG ACCAGCGGGA 
GCTAAGGGCA ACCAGGGCGG CCCCGGCCAG CAGGGCCCTG TAGGAGAGCA GGGGACCAGA 
GGCGCACAGG GTCCGCCTGG TCCCACCGGT CCTCCAGGCC TGATTGGTGA ACAGGGCATT 
CCCGGACCTC GGGGAGGCGG GGGAACCGCA GGTGCTCCTG GAGAACGCGG CAGAACCGGG 
CCCCTGGGAA GAAAGGGTGA GCCCGGAGAT CCAGGACCTA AGGGGAGCAT CGGGAACCGG 
GGTCCTCGTG GGGAGACGGG AGATGATGGG CGAGATGGAG TTGGCAGCGA AGGTCGCATA 
GGCAAGAAGG GAGAAAGAGG ATTCCCTGGA TACCCAGGTC CAAAGGGCAA TCCTGGTGAG 
CCAGGGACAG ACGGACCACC GGGACCCAAA GGCATCAGAG GCCGAAGGGG AAACTCGGGA 
CCTCCAGGGA TAGCTGGACA GAAGGGAGAC CCCGGTTACC CAGGGCCATC TGGTTACAAA 
GGCAGCAGAG GCGACTCAAT GGACCAATGT GCGCTGGTCC AGAGCATCAA AGATAAATGC 
CCTTGCTGTT ACGGGCCCCT GGAGTGCCCC GTCTTCCCGA CGGAGCTGGC TTTCGCCTTG 
GACACCTCAG AGGGGGTCAC CCAGGACAGG TTCAGCCAGA TGAGGGAGGT GGTCCTCAAG 
ATTCTGGACG ACCTGACCAT CGCCGAGAGC AACTGCCCGA GGGGGGCCCG CGTGGCCGTG 
GTCACTTACA ACAACGAGGT TACCACGGAG ATCCGCTTTG CCGACTCCAA GAAGAAGTCC 
GTCCTCCTGG ACAAGATCAA GAACCTGCAG GTGTCGCTGA CCTCCAAGCA GCAGAGCCTG 
GAAACCGCCA TGTCCTTTGT GGCCAGAAAC ACGTTTAAGC GTGTGCGGAG CGGGTTCCTG 
ATGAGGAAGG TGGCGGTGTT CTTCAGCAAC AAGCCCACGA GGGCGACCCC GCAGCTCCGG 
GAGGCTGTGC TCAAGCTCTC AGATGCTGGG ATCACACCCT TGTTCCTCAC AAGCCAGGAG 
GACCGGCAGC TGATCAATGC CCTACAGATC AATAACACAG CGGTGGGACA TGCCCTTGTC 
CTTCCTGCTG GCGGAGACCT CACAGACTTC TTGAAGAATG TCCTGACGTG TCACGTCTGC 
TTGGACATCT GCAACATTGA CCCATCCTGT GGATTCGGCA GCTGGAGGCC TTCCTTCAGG 
GACCGGAGGG CAGCAGGCAG TGATGTGGAC ATCGACATGG CTTTCATCTT AGATAGCTCC 
GAGTCCACCA CCCCGTTCCA GTTCAACGAG ATGAGGAAGT ACATCGAGTA CCTGGTCCGA 
CAGCTGGATG TGAGCCCAGA CCCCAAGGCC TCCCAGCACT TTGCCCGCGT GGCGGTGGTG 
CAGCATGCGC CCTACGAGGC GGTGGACAAC GCCAGTGTGC CGCCTGTGAA GGTGGAGTTC 
TCCCTGACCG ACTACGGCTC CAAAGAGAAG CTGCTGGCCT TCCTTGGCAG CAGGATGACC 
CAGCTGCAGG GGACCAGGGC TTTGGGCCGC GCCATCGACT ACACCATTGA GAACATCTTC 
GAAAGTGCTC CCAACCCCCG GGACCTGAAG ATCGTGGTGC TGATGCTGAC GGGCGAGGTG 
CAGAAGCAGC AGCTGGAGGA GGCCCAGAGA GCCATCCTAC AGGCCAAGTG CAAAGGCTAC 
TTCTTCGTGA TCCTGGGCAT TGGCAGGAAG GTGAATGTCA AGGAGCTGTA CAGCTCTGCC 
AGCGAGCCCA ACGACGTCTT CTTCAAGCTA GTGGACAAGT CGACCGAGCT CAACGAGGAG 
CCCCTAATGC GCTTCGGGAG GCTGCTGCCA TCCTTCGTCA GCAGTAAAGA TGCTTTCTAC 
CTGTCCCCAG ATATCAGGAA ACAGTGTGAT TGGTTCCAAG CAGACCAACC GGCAAAGAAT 
CTCGTGCAGT TTGGTTACAA ACAAATAAAT GTTCCTAATA ACGTTACTTC AAGTCCTACA 
TCTAAACTGG TGACCACAAC GAAGCCGGTG ATCACCACCA CCAAACCGAT CACAGTGGTG 
AACCTGCCAG CCTCAAAGCC AGCTGCCACG AGACCTGTGG ATGAGAGACC CGCAGCTGGG 
AGATCCGTGG CCACCAAGAT GGACAGTGCC AGGCCCGTGG CCACCAAGAC CGAGGCCACC 
AAACCCGTGG CCACCAAGAC CGAGGCCACC AAGACTGAAG CTGCCAAACC TGTGGTCACC 
AAGGCTGAGG CTGCAAAACC CACGGCCACC AAGACTGAAG CCACTAAGAC CGAGGCTGCA 
AAGCTCATGG CCACCAAGAC CGAGGCCACC AAACCTGTGG CTGCCAGGCC CGAGGCGGCA 
AAGACAGCCA CAGCCAGACC TGCAGCAGCC GCGAAGCCTG CAGCAGCCAA ACCTGCCCCT 
GCCAGACCCC CCACTGCTGC CAGATCCATG GCCACGAGGT CCGAGGCCCC CAGGCCCCAG 
GCAGTCAAAC TGGCTGCCAG CAGGCCCGGC GCTGCTAAGC CCGTGGTGAA GGCACCTCGC 
GAGATCCATG TGTCTGAGGT CACAGAGAAC AGCGCCAAGC TGCACTGGGA GAGGCCCGAG 
CCCCCCAGCC CTTACTTATA CAACCTCACC GTCACCTCGG CCCACGATCA GGCCGTGGTT 
CTGAAGCAGA ACCTCACTGT CACGGACCGC GTCATCGGGG GCCTGCTCCC CGGGCAGACT 
TACCACGTTA CGGTGACCTG CTCCCTGAGG TCTCAGGTCA GAGCCATCTA CCAAGGCAGC 
TTCAGCACGA AGAAAATTCA GCCTCCACCT CCACAAACAG AGCGATCAGC CTCTAGTGCA 
ACCATCAACC TAGTGGTGAG CGCAGACCGG TTGGCTGGGA GTAAAGCAGA TATATGTAAG 
TTGCCAAAGG ACGGCGGAAC TTGCAGAGAG TTTGTATTAA AGTGGTACTA TGATTCCGTA 
ACTGAAAACT GCGCCCGATT CTGGTATGGA GGCTGCGGTG GAAACGAAAA CAGATTTAAT 
TCGCAGGATG AATGTGAAAA GGTTTGCCCT CCTGTGCCCA TCAAGCAGCC CGGAGTTATC 
GCCGCGATGG GGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003582086.2
CDS	346..9423
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLERLSIGTQQIRVGVVQYSDEPRTMFSLNSYSTKAQVLDAVKALGFIGGELANVGLALDFVVENHFTRAGGSRAEEGVPQVLVLISAGPSSDEIRDGVIALKQASVFSFGLGAQAASKAELQHIATNDNLVFTVPEFRSLGDVQEQLLPYIVGVAQRHIVLQPPTIVTQVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEVRVNKRDIIFLLDGSSNVGETNFPYVRDFVMNLVNSLDVGSDHIRVGLVQFSDTPVTEFSLNTYPTKSELLAHLRQMQLQGGSVLNTGAALSYVHANHFTEAGGSRIQDHVPQLLLLLTAGQSEDSYLQAANALARAGILTFCVGTSQADRAELEEIAFNPGLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_003582038.4
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003582038.4

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGCA CAAGACTCTG CTGACATTAT TTTCCTTATT 
GATGGATCAA ACAACACCGG AAGTGTCAAT TTCGCAGTCA TTCTCGACTT CCTTGTAAAT 
CTCCTTGAGA GACTGTCAAT TGGAACTCAG CAGATCCGAG TGGGGGTGGT GCAGTATAGC 
GATGAGCCCA GAACCATGTT CTCCTTGAAC AGCTACTCCA CCAAGGCTCA GGTTCTGGAT 
GCAGTGAAGG CCCTGGGGTT CATTGGTGGA GAGCTGGCCA ATGTTGGCCT TGCCCTGGAT 
TTTGTGGTGG AGAATCACTT CACCCGGGCG GGGGGCAGCC GAGCAGAGGA AGGGGTTCCC 
CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATCCGAGA CGGGGTCATA 
GCACTGAAGC AGGCTAGCGT TTTCTCGTTT GGCCTCGGAG CCCAGGCCGC CTCCAAGGCA 
GAGCTTCAGC ACATAGCTAC CAATGACAAC CTGGTGTTTA CTGTCCCGGA ATTCCGTAGC 
CTTGGGGACG TCCAGGAACA ATTACTGCCG TACATTGTTG GCGTGGCCCA AAGGCACATT 
GTCTTGCAAC CGCCAACCAT TGTCACCCAA GTCATTGAAG TCAACAAGAG GGACATAGTT 
TTCCTGGTGG ATGGCTCTTC CAAACTGGGA CTGACCAACT TCAATGCAAT CCGCGACTTC 
ATTGCCAAGG TCATCCAGAG GCTGGAGATC AGACAGGATC TTATCCAGGT GGCAGTGGCT 
CAGTATGCAG ACACAGTGAG GCCAGAGTTT TATTTCAATA CCTACCCCAG CAAGAGGGAG 
GTCATAAACG CCGTGCGGAA AATGAAGGCT TTGGATGGCT CAGCCTTGTA CACGGGCTCC 
GCTCTGGATT TTGTTCGGAA CAACCTGTTC ACGGAGGCGG CCGGCTACCG GGCTGCCGAG 
GGGGTCCCTA AGCTTCTGGT GCTGGTTACA GGCGGTAAGT CCCTAGATGC GGTCAGCCAG 
CCCGCCCAGG AGCTGAAGAG GAGCGGCATC CTGGCCTTCG CCGTCGGGAA CAAGGTGGCT 
GACCAGGCTG AACTGGAAGA GATCGCTTTC GATTCCTCCC TGGTGTTCAC TGCCACCGAG 
TTCCGACCCG CCCCTTTGCA GGGCGTGCTG CCCGGCTTGC TCGGGCCTCT CAGGACCCTC 
ACTGGAACCA CTGAAGTTCG CGTAAACAAA AGGGATATCA TCTTTCTTTT GGATGGATCG 
TCCAACGTCG GAGAGACCAA TTTCCCTTAT GTGCGGGACT TTGTCATGAA CCTAGTTAAC 
AGCCTTGATG TTGGAAGCGA CCACATTCGG GTCGGTCTAG TGCAATTTAG TGACACTCCG 
GTGACGGAGT TCTCCCTAAA CACCTACCCG ACCAAGTCAG AGTTGCTAGC TCACCTGAGG 
CAGATGCAGC TCCAGGGGGG GTCGGTCCTG AACACGGGCG CGGCCCTGAG CTATGTGCAT 
GCCAACCACT TCACCGAAGC TGGCGGCAGC AGGATCCAGG ACCACGTGCC GCAGCTGCTA 
CTCCTGCTCA CGGCCGGGCA GTCTGAGGAC TCCTATTTGC AAGCCGCCAA CGCCCTGGCG 
CGCGCGGGCA TCCTGACCTT TTGTGTGGGC ACTAGCCAGG CCGATAGGGC GGAGCTTGAG 
GAGATTGCTT TTAACCCGGG CCTGGTGTAT CTCATGGATG ATTTCAGCTC CTTGCCAGCT 
TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACTTATGTTA GCGGAGGTGT GGAGGAGGTG 
CCTCTTGCCC AGCCAGAGAG CAAGCGAGAC ATCCTGTTCC TTTTCGACGG CTCAGCCAAC 
CTCATGGGCC AGTTCACTGC CGCCCGTGAC TTTCTCTACA AGGTCATCGA CGAGCTGGAT 
GTGAAACCTG AGGGGACCCG GGTTGCAGTG GCTCAGTTCA GTGATGATGT CAAGGTGGAG 
TCCCGTTTTG ACCAGCACCA GAATAAGCCT GAGATCCTGA ATCTCGTGAA GAGGATGAAG 
CTCAAGACAG GCAAAGCCCT CAACCTGGGC TACGCCTTGG ATTACGCACA GAGGTACATC 
TTTGTGAAGT CCGCTGGAAG CCGGATTGAG GATGGAGTGC TTCAGTTCCT GGTGCTGCTG 
GTGGCGGGAA AGTCATCGGA CCGGGTGGAT ACACCCGCAC TCAACCTGAA GCAGAGCGGG 
GTGGTGCCCT TCATCCTCCA GGCGAAAAAC GCGGACCCTG CTGAGTTGGA GCTGATCGTA 
CCATCCCCAG CCTTTATTTT GGTTGCGGAG TCCCTTCCTA AGATTGGAGA CCTGCAACCA 
CAGATTGTGA ACCTCTTAAA ATCAGTGCAA AATGGGGCAC CGGCACCAGT TTCAGTTGAA 
AAGGATGTGG TGTTCCTGAT TGATGGCTCC GAGGGTGTCA GGAGCGGCTT CCCACTGCTG 
AAGGAGTTCG TCCAGAGAGT GGTGGAGAGC CTGGACGTGG GACCAGACCG GGTGCGTGTG 
GCCGTGGTGC AGTACAGCGA CCGGACCAGA CCTGAGTTCT ACCTGAATTC CTACATGGAC 
CAGCAGAGTG TGGTCGGAGC CATCCGTGGG CTGACCCTGC TGGGCGGGCC GGCCCCCAAC 
ACAGGGGCTG CCCTGGAGTT CGTCCTGAGG AACATCCTGG TGGGCTCTGC TGGGAGCCGC 
ATAGCAGAAG GTGTCCCCCA GCTCCTGATT GTCCTCACGG CCGACAGGTC TGGGGATGAC 
GTGAGGGGCC CCTCTGTGGT CCTGAGGAGG GGAGGGGCCG TGCCCATCGG CATCGGCATT 
GGGAACGCTG ACATCACCGA GATGCAGACC CTCTCCTTCG TCCCGGACTT CGCCGTGGTC 
ATCCCCACCT TCCGGCAGCT GGGGACCATC CAACAGGTCA TCTCGGAGAG AGTGACCCAG 
CTCAGCCGCG AGGAACTGAG CAGGCTGCAG GCGTCTGTCA CCCCCTTGAC GACTCCGGTG 
GTCAGCAGCA AGAGGGACGT GGTCTTTCTC ATCGATGGGT CCCAGAGTGC TGGCCCCGAG 
TTCCAGTACA TCCGAACGCT CATCGAGAGG CTGGTGGACT ACCTGGATGT GGGCTTCGAC 
ACGACCCGGG TGGCGGTCAT CCAGTTCAGC GATGACCCCA GGGTGGAGTT CCTGTTGAAC 
GTCCACTCCA GCAAGGACGA GGTGCAGAAC GCCGTGCGGC GGCTGCGGCC CAAAGGTGGG 
AGGCAGGTCA ACGTTGGGGG TGCCCTGGAG TACGTGGCCA GGAACATCTT CAAGAGGCCC 
CTGGGGAGCC GGATTGAGGA GGGTGTCCCT CAGTTCCTGG TCCTCATTTC CTCCGGGAAG 
TCGGATGATG AGGTGGAGGA CTCGGCCATA GAGCTCAAGC AGTTTGGCGT GGCGCCACTC 
ACGATCGCCA GGAACGTGGA CCAGGAAGAG CTGGTGAAGA TCTCGCTGAG CCCCGAGTAC 
GTGTTCTCAG TGAACACCTT CCGGGAGCTC CCCAGCCTGG AGCAGAAACT GCTGACACCG 
CTCACCACCC TGACTGCCGG GCAGATACAG CAGCTCCTGG CCAGCACGCG CTACCCGCCT 
CCAGCTGTGG AGAGCGATGC TGCGGACATT GTCTTCCTCA TCGACAGCTC TGATGGCGTG 
AAGCCGGATG GCATCGCACA CATCCGGGAC TTTGTTATCA GGATCGTGCG GAGACTCAAC 
GTCGGTCCCA ACAAAGTGAG AATTGGGGTC CTGCAGTTCA GCAACGACGT CTTCCCAGAA 
TTTCAACTAA AGACCTATAA ATCCCAGGCC AGCGTGCTGG ATGCCATACG TCGCCTGAGG 
TTCAAAGGGG GGTCTCCTCT CAACACTGGC AAGGCTCTGG AATTCGTGGC GAGGAATTAC 
TTTGTCAAGT CTGCCGGGAG CCGGATAGAA GATGGGGTGC CCCAACACCT GGTTCTATTT 
CTGGGTGGAA AATCCCAGGA TGATATTTCC AGGTATTCCC AGGTGATAAA ATCTGCAGGA 
ATTGCGAGCT TAGGGGTTGG AGACCGAAAT ATTGACAGAA CAGAGCTGCA GACCATCACC 
AGCGATCCCA GGCTTGTCTT CACGGTGCGG GAGTTCAGAG ACCTCCCCAG CATAGAAGAA 
AGGATGGTGA ATTCCTTTGG GTCCTCCGGG GTCACCCCTG CACCTCCGGG AGTGGACACA 
CCCTCTCCTT CAAGGCCAGA GAAGAAGAAA GCCGACATTG TGTTCCTGCT GGATGGTTCC 
ATCAATTTCC GGAGAGACAG TTTCCAGGAA GTGCTCCGTT TTGTGTCAGA AATTGTGGAC 
ACGGTTTATG AAGGGGGCGA CTCCATCCAG GTGGGGCTGG TCCAATACAA CTCTGACCCC 
ACCGACGAGT TCTTCCTGAA GGACTTCCCC ACCAAGCAGC AGATTATCGA CGCCATAAAC 
AAGGTGGTCT ACAAAGGGGG GAGGCACGCG AACACCAAGG TGGGCCTGGA GCACCTGCGG 
CGGAACCACT TCGTGCCCGA GGCGGGCAGC CGCCTGGACC AGAGGGTCCC GCAGATCGCC 
TTTGTGATCA CGGGGGGCAA GTCGGTGGAG GACGCTCAGG AGGCAAGCAT GGCGCTCACG 
CAGAGAGGCG TCAAGGTGTT TGCGGTGGGC GTAAGGAACA TCGACTCGGA GGAGGTTGGG 
AAGATAGCGT CCAACAGCGC CACGGCCTTC CGGGTGGGGA ACGTCCAGGA GCTGTCGGAA 
CTGAGCGAGC AGGTTTTGGA AACTCTGCAC GATGCAATGC ATGAGACCTT ATGTCCAGGC 
GTGACTGACG TTTCCAAAGC CTGTAACTTG GATGTGATTC TGGGCTTCGA TGGTTCAGGA 
GATCAGAATG TCTTTGTGGC TCAGAAGGGC CTCGAGCCCA AGGTGGACAC CATCCTGAGG 
AGAATCAGTC AGATGCAGAA GATCAGCTGC AGCGGCAGCC AGCTGCCCAC CGTGCGGGTG 
TCTGTGGTGG CCCTCACGCC CTCGGGCCCC GTTGAGGCCT TTGACTTTGC TGAGTACCAG 
CCGGAGCTGT TTGAGAAGTT CCAGAACATG CGTGCCCAGC ACCCCTACGT CCTCACTGCG 
GACACACTGA AGCTCTACCA GAACAAGTTC CGGCAGGCCT CGATGGACAA CGTGAAGGTG 
GTCATTCATT TTACCGATGG AGTGGATGGG GATCTGGCTG ATTTACAAAG AGCATCAGAG 
GAACTCCGAC AAGAAGGTGT CCGAGCTCTG ATCTTGGTGG GCCTCGAACG CGTCGCCAAC 
TTGGAGCAGC TCATGCAGCT GGAGTTCGGG CGAGGGTTCA CGTACAACCG GCCCCTGAGG 
CTGAACTTGC TGGACCTGGA CTACGAACTA GCCGAGCAGC TTGACAACAT TGCTGAGAAA 
GCCTGCTGTG GGATTCCCTG CAAATGCTCT GGACAAAGAG GAGACCGAGG GCCCATAGGC 
AGCATCGGGC CAAAGGGCAT TCCTGGAGAA GATGGCTACC GAGGTTATCC TGGTGACGAA 
GGTGGACCTG GTGAACGCGG TCCTCCTGGC GTGAACGGCA CTCAAGGTTT CCAGGGCTGC 
CCCGGGCAGA GAGGAGTCAA GGGCTCTCGT GGATTCCCGG GAGAGAAGGG TGAATTAGGA 
GAAATTGGAC TGGATGGTCT TGATGGTGAA GATGGCGACA AAGGGCTGCC CGGTACTTCT 
GGAGAGAAAG GGAGTCCAGG AAGGAGGGGC GACAAAGGAC CCAAAGGCGA CAAAGGGGAG 
AGAGGCGATG TCGGGATCAG AGGCGACCCG GGCAATTCAG GACAGGACAG CCAGCAGAGA 
GGAGCCAAAG GAGAAACCGG AGACATCGGC CCCGTGGGTC TCCCTGGGAG AGACGGGGTG 
TCTGGAGGGC CTGGAGAACC CGGGAAGAGC GGTGGCTTTG GCCGAAGGGG ACCAGCGGGA 
GCTAAGGGCA ACCAGGGCGG CCCCGGCCAG CAGGGCCCTG TAGGAGAGCA GGGGACCAGA 
GGCGCACAGG GTCCGCCTGG TCCCACCGGT CCTCCAGGCC TGATTGGTGA ACAGGGCATT 
CCCGGACCTC GGGGAGGCGG GGGAACCGCA GGTGCTCCTG GAGAACGCGG CAGAACCGGG 
CCCCTGGGAA GAAAGGGTGA GCCCGGAGAT CCAGGACCTA AGGGGAGCAT CGGGAACCGG 
GGTCCTCGTG GGGAGACGGG AGATGATGGG CGAGATGGAG TTGGCAGCGA AGGTCGCATA 
GGCAAGAAGG GAGAAAGAGG ATTCCCTGGA TACCCAGGTC CAAAGGGCAA TCCTGGTGAG 
CCAGGGACAG ACGGACCACC GGGACCCAAA GGCATCAGAG GCCGAAGGGG AAACTCGGGA 
CCTCCAGGGA TAGCTGGACA GAAGGGAGAC CCCGGTTACC CAGGGCCATC TGGTTACAAA 
GGCAGCAGAG GCGACTCAAT GGACCAATGT GCGCTGGTCC AGAGCATCAA AGATAAATGC 
CCTTGCTGTT ACGGGCCCCT GGAGTGCCCC GTCTTCCCGA CGGAGCTGGC TTTCGCCTTG 
GACACCTCAG AGGGGGTCAC CCAGGACAGG TTCAGCCAGA TGAGGGAGGT GGTCCTCAAG 
ATTCTGGACG ACCTGACCAT CGCCGAGAGC AACTGCCCGA GGGGGGCCCG CGTGGCCGTG 
GTCACTTACA ACAACGAGGT TACCACGGAG ATCCGCTTTG CCGACTCCAA GAAGAAGTCC 
GTCCTCCTGG ACAAGATCAA GAACCTGCAG GTGTCGCTGA CCTCCAAGCA GCAGAGCCTG 
GAAACCGCCA TGTCCTTTGT GGCCAGAAAC ACGTTTAAGC GTGTGCGGAG CGGGTTCCTG 
ATGAGGAAGG TGGCGGTGTT CTTCAGCAAC AAGCCCACGA GGGCGACCCC GCAGCTCCGG 
GAGGCTGTGC TCAAGCTCTC AGATGCTGGG ATCACACCCT TGTTCCTCAC AAGCCAGGAG 
GACCGGCAGC TGATCAATGC CCTACAGATC AATAACACAG CGGTGGGACA TGCCCTTGTC 
CTTCCTGCTG GCGGAGACCT CACAGACTTC TTGAAGAATG TCCTGACGTG TCACGTCTGC 
TTGGACATCT GCAACATTGA CCCATCCTGT GGATTCGGCA GCTGGAGGCC TTCCTTCAGG 
GACCGGAGGG CAGCAGGCAG TGATGTGGAC ATCGACATGG CTTTCATCTT AGATAGCTCC 
GAGTCCACCA CCCCGTTCCA GTTCAACGAG ATGAGGAAGT ACATCGAGTA CCTGGTCCGA 
CAGCTGGATG TGAGCCCAGA CCCCAAGGCC TCCCAGCACT TTGCCCGCGT GGCGGTGGTG 
CAGCATGCGC CCTACGAGGC GGTGGACAAC GCCAGTGTGC CGCCTGTGAA GGTGGAGTTC 
TCCCTGACCG ACTACGGCTC CAAAGAGAAG CTGCTGGCCT TCCTTGGCAG CAGGATGACC 
CAGCTGCAGG GGACCAGGGC TTTGGGCCGC GCCATCGACT ACACCATTGA GAACATCTTC 
GAAAGTGCTC CCAACCCCCG GGACCTGAAG ATCGTGGTGC TGATGCTGAC GGGCGAGGTG 
CAGAAGCAGC AGCTGGAGGA GGCCCAGAGA GCCATCCTAC AGGCCAAGTG CAAAGGCTAC 
TTCTTCGTGA TCCTGGGCAT TGGCAGGAAG GTGAATGTCA AGGAGCTGTA CAGCTCTGCC 
AGCGAGCCCA ACGACGTCTT CTTCAAGCTA GTGGACAAGT CGACCGAGCT CAACGAGGAG 
CCCCTAATGC GCTTCGGGAG GCTGCTGCCA TCCTTCGTCA GCAGTAAAGA TGCTTTCTAC 
CTGTCCCCAG ATATCAGGAA ACAGTGTGAT TGGTTCCAAG CAGACCAACC GGCAAAGAAT 
CTCGTGCAGT TTGGTTACAA ACAAATAAAT GTTCCTAATA ACGTTACTTC AAGTCCTACA 
TCTAAACTGG TGACCACAAC GAAGCCGGTG ATCACCACCA CCAAACCGAT CACAGTGGTG 
AACCTGCCAG CCTCAAAGCC AGCTGCCACG AGACCTGTGG ATGAGAGACC CGCAGCTGGG 
AGATCCGTGG CCACCAAGAT GGACAGTGCC AGGCCCGTGG CCACCAAGAC CGAGGCCACC 
AAACCCGTGG CCACCAAGAC CGAGGCCACC AAGACTGAAG CTGCCAAACC TGTGGTCACC 
AAGGCTGAGG CTGCAAAACC CACGGCCACC AAGACTGAAG CCACTAAGAC CGAGGCTGCA 
AAGCTCATGG CCACCAAGAC CGAGGCCACC AAACCTGTGG CTGCCAGGCC CGAGGCGGCA 
AAGACAGCCA CAGCCAGACC TGCAGCAGCC GCGAAGCCTG CAGCAGCCAA ACCTGCCCCT 
GCCAGACCCC CCACTGCTGC CAGATCCATG GCCACGAGGT CCGAGGCCCC CAGGCCCCAG 
GCAGTCAAAC TGGCTGCCAG CAGGCCCGGC GCTGCTAAGC CCGTGGTGAA GGCACCTCGC 
GAGATCCATG TGTCTGAGGT CACAGAGAAC AGCGCCAAGC TGCACTGGGA GAGGCCCGAG 
CCCCCCAGCC CTTACTTATA CAACCTCACC GTCACCTCGG CCCACGATCA GGCCGTGGTT 
CTGAAGCAGA ACCTCACTGT CACGGACCGC GTCATCGGGG GCCTGCTCCC CGGGCAGACT 
TACCACGTTA CGGTGACCTG CTCCCTGAGG TCTCAGGTCA GAGCCATCTA CCAAGGCAGC 
TTCAGCACGA AGAAAATTCA GCCTCCACCT CCACAAACAG AGCGATCAGC CTCTAGTGCA 
ACCATCAACC TAGTGGTGAG CGCAGACCGG TTGGCTGGGA GTAAAGCAGA TATATGTAAG 
TTGCCAAAGG ACGGCGGAAC TTGCAGAGAG TTTGTATTAA AGTGGTACTA TGATTCCGTA 
ACTGAAAACT GCGCCCGATT CTGGTATGGA GGCTGCGGTG GAAACGAAAA CAGATTTAAT 
TCGCAGGATG AATGTGAAAA GGTTTGCCCT CCTGTGCCCA TCAAGCAGCC CGGAGTTATC 
GCCGCGATGG GGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_002686571.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002686617.2 Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X3, mRNA.

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CloneID	OBa03698
Clone ID Related Accession (Same CDS sequence)	XM_003582038.4 , XM_002686571.5
Accession Version	XM_002686571.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002686571.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGCA CAAGACTCTG CTGACATTAT TTTCCTTATT 
GATGGATCAA ACAACACCGG AAGTGTCAAT TTCGCAGTCA TTCTCGACTT CCTTGTAAAT 
CTCCTTGAGA GACTGTCAAT TGGAACTCAG CAGATCCGAG TGGGGGTGGT GCAGTATAGC 
GATGAGCCCA GAACCATGTT CTCCTTGAAC AGCTACTCCA CCAAGGCTCA GGTTCTGGAT 
GCAGTGAAGG CCCTGGGGTT CATTGGTGGA GAGCTGGCCA ATGTTGGCCT TGCCCTGGAT 
TTTGTGGTGG AGAATCACTT CACCCGGGCG GGGGGCAGCC GAGCAGAGGA AGGGGTTCCC 
CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATCCGAGA CGGGGTCATA 
GCACTGAAGC AGGCTAGCGT TTTCTCGTTT GGCCTCGGAG CCCAGGCCGC CTCCAAGGCA 
GAGCTTCAGC ACATAGCTAC CAATGACAAC CTGGTGTTTA CTGTCCCGGA ATTCCGTAGC 
CTTGGGGACG TCCAGGAACA ATTACTGCCG TACATTGTTG GCGTGGCCCA AAGGCACATT 
GTCTTGCAAC CGCCAACCAT TGTCACCCAA GTCATTGAAG TCAACAAGAG GGACATAGTT 
TTCCTGGTGG ATGGCTCTTC CAAACTGGGA CTGACCAACT TCAATGCAAT CCGCGACTTC 
ATTGCCAAGG TCATCCAGAG GCTGGAGATC AGACAGGATC TTATCCAGGT GGCAGTGGCT 
CAGTATGCAG ACACAGTGAG GCCAGAGTTT TATTTCAATA CCTACCCCAG CAAGAGGGAG 
GTCATAAACG CCGTGCGGAA AATGAAGGCT TTGGATGGCT CAGCCTTGTA CACGGGCTCC 
GCTCTGGATT TTGTTCGGAA CAACCTGTTC ACGGAGGCGG CCGGCTACCG GGCTGCCGAG 
GGGGTCCCTA AGCTTCTGGT GCTGGTTACA GGCGGTAAGT CCCTAGATGC GGTCAGCCAG 
CCCGCCCAGG AGCTGAAGAG GAGCGGCATC CTGGCCTTCG CCGTCGGGAA CAAGGTGGCT 
GACCAGGCTG AACTGGAAGA GATCGCTTTC GATTCCTCCC TGGTGTTCAC TGCCACCGAG 
TTCCGACCCG CCCCTTTGCA GGGCGTGCTG CCCGGCTTGC TCGGGCCTCT CAGGACCCTC 
ACTGGAACCA CTGAAGTTCG CGTAAACAAA AGGGATATCA TCTTTCTTTT GGATGGATCG 
TCCAACGTCG GAGAGACCAA TTTCCCTTAT GTGCGGGACT TTGTCATGAA CCTAGTTAAC 
AGCCTTGATG TTGGAAGCGA CCACATTCGG GTCGGTCTAG TGCAATTTAG TGACACTCCG 
GTGACGGAGT TCTCCCTAAA CACCTACCCG ACCAAGTCAG AGTTGCTAGC TCACCTGAGG 
CAGATGCAGC TCCAGGGGGG GTCGGTCCTG AACACGGGCG CGGCCCTGAG CTATGTGCAT 
GCCAACCACT TCACCGAAGC TGGCGGCAGC AGGATCCAGG ACCACGTGCC GCAGCTGCTA 
CTCCTGCTCA CGGCCGGGCA GTCTGAGGAC TCCTATTTGC AAGCCGCCAA CGCCCTGGCG 
CGCGCGGGCA TCCTGACCTT TTGTGTGGGC ACTAGCCAGG CCGATAGGGC GGAGCTTGAG 
GAGATTGCTT TTAACCCGGG CCTGGTGTAT CTCATGGATG ATTTCAGCTC CTTGCCAGCT 
TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACTTATGTTA GCGGAGGTGT GGAGGAGGTG 
CCTCTTGCCC AGCCAGAGAG CAAGCGAGAC ATCCTGTTCC TTTTCGACGG CTCAGCCAAC 
CTCATGGGCC AGTTCACTGC CGCCCGTGAC TTTCTCTACA AGGTCATCGA CGAGCTGGAT 
GTGAAACCTG AGGGGACCCG GGTTGCAGTG GCTCAGTTCA GTGATGATGT CAAGGTGGAG 
TCCCGTTTTG ACCAGCACCA GAATAAGCCT GAGATCCTGA ATCTCGTGAA GAGGATGAAG 
CTCAAGACAG GCAAAGCCCT CAACCTGGGC TACGCCTTGG ATTACGCACA GAGGTACATC 
TTTGTGAAGT CCGCTGGAAG CCGGATTGAG GATGGAGTGC TTCAGTTCCT GGTGCTGCTG 
GTGGCGGGAA AGTCATCGGA CCGGGTGGAT ACACCCGCAC TCAACCTGAA GCAGAGCGGG 
GTGGTGCCCT TCATCCTCCA GGCGAAAAAC GCGGACCCTG CTGAGTTGGA GCTGATCGTA 
CCATCCCCAG CCTTTATTTT GGTTGCGGAG TCCCTTCCTA AGATTGGAGA CCTGCAACCA 
CAGATTGTGA ACCTCTTAAA ATCAGTGCAA AATGGGGCAC CGGCACCAGT TTCAGTTGAA 
AAGGATGTGG TGTTCCTGAT TGATGGCTCC GAGGGTGTCA GGAGCGGCTT CCCACTGCTG 
AAGGAGTTCG TCCAGAGAGT GGTGGAGAGC CTGGACGTGG GACCAGACCG GGTGCGTGTG 
GCCGTGGTGC AGTACAGCGA CCGGACCAGA CCTGAGTTCT ACCTGAATTC CTACATGGAC 
CAGCAGAGTG TGGTCGGAGC CATCCGTGGG CTGACCCTGC TGGGCGGGCC GGCCCCCAAC 
ACAGGGGCTG CCCTGGAGTT CGTCCTGAGG AACATCCTGG TGGGCTCTGC TGGGAGCCGC 
ATAGCAGAAG GTGTCCCCCA GCTCCTGATT GTCCTCACGG CCGACAGGTC TGGGGATGAC 
GTGAGGGGCC CCTCTGTGGT CCTGAGGAGG GGAGGGGCCG TGCCCATCGG CATCGGCATT 
GGGAACGCTG ACATCACCGA GATGCAGACC CTCTCCTTCG TCCCGGACTT CGCCGTGGTC 
ATCCCCACCT TCCGGCAGCT GGGGACCATC CAACAGGTCA TCTCGGAGAG AGTGACCCAG 
CTCAGCCGCG AGGAACTGAG CAGGCTGCAG GCGTCTGTCA CCCCCTTGAC GACTCCGGTG 
GTCAGCAGCA AGAGGGACGT GGTCTTTCTC ATCGATGGGT CCCAGAGTGC TGGCCCCGAG 
TTCCAGTACA TCCGAACGCT CATCGAGAGG CTGGTGGACT ACCTGGATGT GGGCTTCGAC 
ACGACCCGGG TGGCGGTCAT CCAGTTCAGC GATGACCCCA GGGTGGAGTT CCTGTTGAAC 
GTCCACTCCA GCAAGGACGA GGTGCAGAAC GCCGTGCGGC GGCTGCGGCC CAAAGGTGGG 
AGGCAGGTCA ACGTTGGGGG TGCCCTGGAG TACGTGGCCA GGAACATCTT CAAGAGGCCC 
CTGGGGAGCC GGATTGAGGA GGGTGTCCCT CAGTTCCTGG TCCTCATTTC CTCCGGGAAG 
TCGGATGATG AGGTGGAGGA CTCGGCCATA GAGCTCAAGC AGTTTGGCGT GGCGCCACTC 
ACGATCGCCA GGAACGTGGA CCAGGAAGAG CTGGTGAAGA TCTCGCTGAG CCCCGAGTAC 
GTGTTCTCAG TGAACACCTT CCGGGAGCTC CCCAGCCTGG AGCAGAAACT GCTGACACCG 
CTCACCACCC TGACTGCCGG GCAGATACAG CAGCTCCTGG CCAGCACGCG CTACCCGCCT 
CCAGCTGTGG AGAGCGATGC TGCGGACATT GTCTTCCTCA TCGACAGCTC TGATGGCGTG 
AAGCCGGATG GCATCGCACA CATCCGGGAC TTTGTTATCA GGATCGTGCG GAGACTCAAC 
GTCGGTCCCA ACAAAGTGAG AATTGGGGTC CTGCAGTTCA GCAACGACGT CTTCCCAGAA 
TTTCAACTAA AGACCTATAA ATCCCAGGCC AGCGTGCTGG ATGCCATACG TCGCCTGAGG 
TTCAAAGGGG GGTCTCCTCT CAACACTGGC AAGGCTCTGG AATTCGTGGC GAGGAATTAC 
TTTGTCAAGT CTGCCGGGAG CCGGATAGAA GATGGGGTGC CCCAACACCT GGTTCTATTT 
CTGGGTGGAA AATCCCAGGA TGATATTTCC AGGTATTCCC AGGTGATAAA ATCTGCAGGA 
ATTGCGAGCT TAGGGGTTGG AGACCGAAAT ATTGACAGAA CAGAGCTGCA GACCATCACC 
AGCGATCCCA GGCTTGTCTT CACGGTGCGG GAGTTCAGAG ACCTCCCCAG CATAGAAGAA 
AGGATGGTGA ATTCCTTTGG GTCCTCCGGG GTCACCCCTG CACCTCCGGG AGTGGACACA 
CCCTCTCCTT CAAGGCCAGA GAAGAAGAAA GCCGACATTG TGTTCCTGCT GGATGGTTCC 
ATCAATTTCC GGAGAGACAG TTTCCAGGAA GTGCTCCGTT TTGTGTCAGA AATTGTGGAC 
ACGGTTTATG AAGGGGGCGA CTCCATCCAG GTGGGGCTGG TCCAATACAA CTCTGACCCC 
ACCGACGAGT TCTTCCTGAA GGACTTCCCC ACCAAGCAGC AGATTATCGA CGCCATAAAC 
AAGGTGGTCT ACAAAGGGGG GAGGCACGCG AACACCAAGG TGGGCCTGGA GCACCTGCGG 
CGGAACCACT TCGTGCCCGA GGCGGGCAGC CGCCTGGACC AGAGGGTCCC GCAGATCGCC 
TTTGTGATCA CGGGGGGCAA GTCGGTGGAG GACGCTCAGG AGGCAAGCAT GGCGCTCACG 
CAGAGAGGCG TCAAGGTGTT TGCGGTGGGC GTAAGGAACA TCGACTCGGA GGAGGTTGGG 
AAGATAGCGT CCAACAGCGC CACGGCCTTC CGGGTGGGGA ACGTCCAGGA GCTGTCGGAA 
CTGAGCGAGC AGGTTTTGGA AACTCTGCAC GATGCAATGC ATGAGACCTT ATGTCCAGGC 
GTGACTGACG TTTCCAAAGC CTGTAACTTG GATGTGATTC TGGGCTTCGA TGGTTCAGGA 
GATCAGAATG TCTTTGTGGC TCAGAAGGGC CTCGAGCCCA AGGTGGACAC CATCCTGAGG 
AGAATCAGTC AGATGCAGAA GATCAGCTGC AGCGGCAGCC AGCTGCCCAC CGTGCGGGTG 
TCTGTGGTGG CCCTCACGCC CTCGGGCCCC GTTGAGGCCT TTGACTTTGC TGAGTACCAG 
CCGGAGCTGT TTGAGAAGTT CCAGAACATG CGTGCCCAGC ACCCCTACGT CCTCACTGCG 
GACACACTGA AGCTCTACCA GAACAAGTTC CGGCAGGCCT CGATGGACAA CGTGAAGGTG 
GTCATTCATT TTACCGATGG AGTGGATGGG GATCTGGCTG ATTTACAAAG AGCATCAGAG 
GAACTCCGAC AAGAAGGTGT CCGAGCTCTG ATCTTGGTGG GCCTCGAACG CGTCGCCAAC 
TTGGAGCAGC TCATGCAGCT GGAGTTCGGG CGAGGGTTCA CGTACAACCG GCCCCTGAGG 
CTGAACTTGC TGGACCTGGA CTACGAACTA GCCGAGCAGC TTGACAACAT TGCTGAGAAA 
GCCTGCTGTG GGATTCCCTG CAAATGCTCT GGACAAAGAG GAGACCGAGG GCCCATAGGC 
AGCATCGGGC CAAAGGGCAT TCCTGGAGAA GATGGCTACC GAGGTTATCC TGGTGACGAA 
GGTGGACCTG GTGAACGCGG TCCTCCTGGC GTGAACGGCA CTCAAGGTTT CCAGGGCTGC 
CCCGGGCAGA GAGGAGTCAA GGGCTCTCGT GGATTCCCGG GAGAGAAGGG TGAATTAGGA 
GAAATTGGAC TGGATGGTCT TGATGGTGAA GATGGCGACA AAGGGCTGCC CGGTACTTCT 
GGAGAGAAAG GGAGTCCAGG AAGGAGGGGC GACAAAGGAC CCAAAGGCGA CAAAGGGGAG 
AGAGGCGATG TCGGGATCAG AGGCGACCCG GGCAATTCAG GACAGGACAG CCAGCAGAGA 
GGAGCCAAAG GAGAAACCGG AGACATCGGC CCCGTGGGTC TCCCTGGGAG AGACGGGGTG 
TCTGGAGGGC CTGGAGAACC CGGGAAGAGC GGTGGCTTTG GCCGAAGGGG ACCAGCGGGA 
GCTAAGGGCA ACCAGGGCGG CCCCGGCCAG CAGGGCCCTG TAGGAGAGCA GGGGACCAGA 
GGCGCACAGG GTCCGCCTGG TCCCACCGGT CCTCCAGGCC TGATTGGTGA ACAGGGCATT 
CCCGGACCTC GGGGAGGCGG GGGAACCGCA GGTGCTCCTG GAGAACGCGG CAGAACCGGG 
CCCCTGGGAA GAAAGGGTGA GCCCGGAGAT CCAGGACCTA AGGGGAGCAT CGGGAACCGG 
GGTCCTCGTG GGGAGACGGG AGATGATGGG CGAGATGGAG TTGGCAGCGA AGGTCGCATA 
GGCAAGAAGG GAGAAAGAGG ATTCCCTGGA TACCCAGGTC CAAAGGGCAA TCCTGGTGAG 
CCAGGGACAG ACGGACCACC GGGACCCAAA GGCATCAGAG GCCGAAGGGG AAACTCGGGA 
CCTCCAGGGA TAGCTGGACA GAAGGGAGAC CCCGGTTACC CAGGGCCATC TGGTTACAAA 
GGCAGCAGAG GCGACTCAAT GGACCAATGT GCGCTGGTCC AGAGCATCAA AGATAAATGC 
CCTTGCTGTT ACGGGCCCCT GGAGTGCCCC GTCTTCCCGA CGGAGCTGGC TTTCGCCTTG 
GACACCTCAG AGGGGGTCAC CCAGGACAGG TTCAGCCAGA TGAGGGAGGT GGTCCTCAAG 
ATTCTGGACG ACCTGACCAT CGCCGAGAGC AACTGCCCGA GGGGGGCCCG CGTGGCCGTG 
GTCACTTACA ACAACGAGGT TACCACGGAG ATCCGCTTTG CCGACTCCAA GAAGAAGTCC 
GTCCTCCTGG ACAAGATCAA GAACCTGCAG GTGTCGCTGA CCTCCAAGCA GCAGAGCCTG 
GAAACCGCCA TGTCCTTTGT GGCCAGAAAC ACGTTTAAGC GTGTGCGGAG CGGGTTCCTG 
ATGAGGAAGG TGGCGGTGTT CTTCAGCAAC AAGCCCACGA GGGCGACCCC GCAGCTCCGG 
GAGGCTGTGC TCAAGCTCTC AGATGCTGGG ATCACACCCT TGTTCCTCAC AAGCCAGGAG 
GACCGGCAGC TGATCAATGC CCTACAGATC AATAACACAG CGGTGGGACA TGCCCTTGTC 
CTTCCTGCTG GCGGAGACCT CACAGACTTC TTGAAGAATG TCCTGACGTG TCACGTCTGC 
TTGGACATCT GCAACATTGA CCCATCCTGT GGATTCGGCA GCTGGAGGCC TTCCTTCAGG 
GACCGGAGGG CAGCAGGCAG TGATGTGGAC ATCGACATGG CTTTCATCTT AGATAGCTCC 
GAGTCCACCA CCCCGTTCCA GTTCAACGAG ATGAGGAAGT ACATCGAGTA CCTGGTCCGA 
CAGCTGGATG TGAGCCCAGA CCCCAAGGCC TCCCAGCACT TTGCCCGCGT GGCGGTGGTG 
CAGCATGCGC CCTACGAGGC GGTGGACAAC GCCAGTGTGC CGCCTGTGAA GGTGGAGTTC 
TCCCTGACCG ACTACGGCTC CAAAGAGAAG CTGCTGGCCT TCCTTGGCAG CAGGATGACC 
CAGCTGCAGG GGACCAGGGC TTTGGGCCGC GCCATCGACT ACACCATTGA GAACATCTTC 
GAAAGTGCTC CCAACCCCCG GGACCTGAAG ATCGTGGTGC TGATGCTGAC GGGCGAGGTG 
CAGAAGCAGC AGCTGGAGGA GGCCCAGAGA GCCATCCTAC AGGCCAAGTG CAAAGGCTAC 
TTCTTCGTGA TCCTGGGCAT TGGCAGGAAG GTGAATGTCA AGGAGCTGTA CAGCTCTGCC 
AGCGAGCCCA ACGACGTCTT CTTCAAGCTA GTGGACAAGT CGACCGAGCT CAACGAGGAG 
CCCCTAATGC GCTTCGGGAG GCTGCTGCCA TCCTTCGTCA GCAGTAAAGA TGCTTTCTAC 
CTGTCCCCAG ATATCAGGAA ACAGTGTGAT TGGTTCCAAG CAGACCAACC GGCAAAGAAT 
CTCGTGCAGT TTGGTTACAA ACAAATAAAT GTTCCTAATA ACGTTACTTC AAGTCCTACA 
TCTAAACTGG TGACCACAAC GAAGCCGGTG ATCACCACCA CCAAACCGAT CACAGTGGTG 
AACCTGCCAG CCTCAAAGCC AGCTGCCACG AGACCTGTGG ATGAGAGACC CGCAGCTGGG 
AGATCCGTGG CCACCAAGAT GGACAGTGCC AGGCCCGTGG CCACCAAGAC CGAGGCCACC 
AAACCCGTGG CCACCAAGAC CGAGGCCACC AAGACTGAAG CTGCCAAACC TGTGGTCACC 
AAGGCTGAGG CTGCAAAACC CACGGCCACC AAGACTGAAG CCACTAAGAC CGAGGCTGCA 
AAGCTCATGG CCACCAAGAC CGAGGCCACC AAACCTGTGG CTGCCAGGCC CGAGGCGGCA 
AAGACAGCCA CAGCCAGACC TGCAGCAGCC GCGAAGCCTG CAGCAGCCAA ACCTGCCCCT 
GCCAGACCCC CCACTGCTGC CAGATCCATG GCCACGAGGT CCGAGGCCCC CAGGCCCCAG 
GCAGTCAAAC TGGCTGCCAG CAGGCCCGGC GCTGCTAAGC CCGTGGTGAA GGCACCTCGC 
GAGATCCATG TGTCTGAGGT CACAGAGAAC AGCGCCAAGC TGCACTGGGA GAGGCCCGAG 
CCCCCCAGCC CTTACTTATA CAACCTCACC GTCACCTCGG CCCACGATCA GGCCGTGGTT 
CTGAAGCAGA ACCTCACTGT CACGGACCGC GTCATCGGGG GCCTGCTCCC CGGGCAGACT 
TACCACGTTA CGGTGACCTG CTCCCTGAGG TCTCAGGTCA GAGCCATCTA CCAAGGCAGC 
TTCAGCACGA AGAAAATTCA GCCTCCACCT CCACAAACAG AGCGATCAGC CTCTAGTGCA 
ACCATCAACC TAGTGGTGAG CGCAGACCGG TTGGCTGGGA GTAAAGCAGA TATATGTAAG 
TTGCCAAAGG ACGGCGGAAC TTGCAGAGAG TTTGTATTAA AGTGGTACTA TGATTCCGTA 
ACTGAAAACT GCGCCCGATT CTGGTATGGA GGCTGCGGTG GAAACGAAAA CAGATTTAAT 
TCGCAGGATG AATGTGAAAA GGTTTGCCCT CCTGTGCCCA TCAAGCAGCC CGGAGTTATC 
GCCGCGATGG GGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002686617.2
CDS	346..9423
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLERLSIGTQQIRVGVVQYSDEPRTMFSLNSYSTKAQVLDAVKALGFIGGELANVGLALDFVVENHFTRAGGSRAEEGVPQVLVLISAGPSSDEIRDGVIALKQASVFSFGLGAQAASKAELQHIATNDNLVFTVPEFRSLGDVQEQLLPYIVGVAQRHIVLQPPTIVTQVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEVRVNKRDIIFLLDGSSNVGETNFPYVRDFVMNLVNSLDVGSDHIRVGLVQFSDTPVTEFSLNTYPTKSELLAHLRQMQLQGGSVLNTGAALSYVHANHFTEAGGSRIQDHVPQLLLLLTAGQSEDSYLQAANALARAGILTFCVGTSQADRAELEEIAFNPGLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_002686571.5
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002686571.5

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGCA CAAGACTCTG CTGACATTAT TTTCCTTATT 
GATGGATCAA ACAACACCGG AAGTGTCAAT TTCGCAGTCA TTCTCGACTT CCTTGTAAAT 
CTCCTTGAGA GACTGTCAAT TGGAACTCAG CAGATCCGAG TGGGGGTGGT GCAGTATAGC 
GATGAGCCCA GAACCATGTT CTCCTTGAAC AGCTACTCCA CCAAGGCTCA GGTTCTGGAT 
GCAGTGAAGG CCCTGGGGTT CATTGGTGGA GAGCTGGCCA ATGTTGGCCT TGCCCTGGAT 
TTTGTGGTGG AGAATCACTT CACCCGGGCG GGGGGCAGCC GAGCAGAGGA AGGGGTTCCC 
CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATCCGAGA CGGGGTCATA 
GCACTGAAGC AGGCTAGCGT TTTCTCGTTT GGCCTCGGAG CCCAGGCCGC CTCCAAGGCA 
GAGCTTCAGC ACATAGCTAC CAATGACAAC CTGGTGTTTA CTGTCCCGGA ATTCCGTAGC 
CTTGGGGACG TCCAGGAACA ATTACTGCCG TACATTGTTG GCGTGGCCCA AAGGCACATT 
GTCTTGCAAC CGCCAACCAT TGTCACCCAA GTCATTGAAG TCAACAAGAG GGACATAGTT 
TTCCTGGTGG ATGGCTCTTC CAAACTGGGA CTGACCAACT TCAATGCAAT CCGCGACTTC 
ATTGCCAAGG TCATCCAGAG GCTGGAGATC AGACAGGATC TTATCCAGGT GGCAGTGGCT 
CAGTATGCAG ACACAGTGAG GCCAGAGTTT TATTTCAATA CCTACCCCAG CAAGAGGGAG 
GTCATAAACG CCGTGCGGAA AATGAAGGCT TTGGATGGCT CAGCCTTGTA CACGGGCTCC 
GCTCTGGATT TTGTTCGGAA CAACCTGTTC ACGGAGGCGG CCGGCTACCG GGCTGCCGAG 
GGGGTCCCTA AGCTTCTGGT GCTGGTTACA GGCGGTAAGT CCCTAGATGC GGTCAGCCAG 
CCCGCCCAGG AGCTGAAGAG GAGCGGCATC CTGGCCTTCG CCGTCGGGAA CAAGGTGGCT 
GACCAGGCTG AACTGGAAGA GATCGCTTTC GATTCCTCCC TGGTGTTCAC TGCCACCGAG 
TTCCGACCCG CCCCTTTGCA GGGCGTGCTG CCCGGCTTGC TCGGGCCTCT CAGGACCCTC 
ACTGGAACCA CTGAAGTTCG CGTAAACAAA AGGGATATCA TCTTTCTTTT GGATGGATCG 
TCCAACGTCG GAGAGACCAA TTTCCCTTAT GTGCGGGACT TTGTCATGAA CCTAGTTAAC 
AGCCTTGATG TTGGAAGCGA CCACATTCGG GTCGGTCTAG TGCAATTTAG TGACACTCCG 
GTGACGGAGT TCTCCCTAAA CACCTACCCG ACCAAGTCAG AGTTGCTAGC TCACCTGAGG 
CAGATGCAGC TCCAGGGGGG GTCGGTCCTG AACACGGGCG CGGCCCTGAG CTATGTGCAT 
GCCAACCACT TCACCGAAGC TGGCGGCAGC AGGATCCAGG ACCACGTGCC GCAGCTGCTA 
CTCCTGCTCA CGGCCGGGCA GTCTGAGGAC TCCTATTTGC AAGCCGCCAA CGCCCTGGCG 
CGCGCGGGCA TCCTGACCTT TTGTGTGGGC ACTAGCCAGG CCGATAGGGC GGAGCTTGAG 
GAGATTGCTT TTAACCCGGG CCTGGTGTAT CTCATGGATG ATTTCAGCTC CTTGCCAGCT 
TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACTTATGTTA GCGGAGGTGT GGAGGAGGTG 
CCTCTTGCCC AGCCAGAGAG CAAGCGAGAC ATCCTGTTCC TTTTCGACGG CTCAGCCAAC 
CTCATGGGCC AGTTCACTGC CGCCCGTGAC TTTCTCTACA AGGTCATCGA CGAGCTGGAT 
GTGAAACCTG AGGGGACCCG GGTTGCAGTG GCTCAGTTCA GTGATGATGT CAAGGTGGAG 
TCCCGTTTTG ACCAGCACCA GAATAAGCCT GAGATCCTGA ATCTCGTGAA GAGGATGAAG 
CTCAAGACAG GCAAAGCCCT CAACCTGGGC TACGCCTTGG ATTACGCACA GAGGTACATC 
TTTGTGAAGT CCGCTGGAAG CCGGATTGAG GATGGAGTGC TTCAGTTCCT GGTGCTGCTG 
GTGGCGGGAA AGTCATCGGA CCGGGTGGAT ACACCCGCAC TCAACCTGAA GCAGAGCGGG 
GTGGTGCCCT TCATCCTCCA GGCGAAAAAC GCGGACCCTG CTGAGTTGGA GCTGATCGTA 
CCATCCCCAG CCTTTATTTT GGTTGCGGAG TCCCTTCCTA AGATTGGAGA CCTGCAACCA 
CAGATTGTGA ACCTCTTAAA ATCAGTGCAA AATGGGGCAC CGGCACCAGT TTCAGTTGAA 
AAGGATGTGG TGTTCCTGAT TGATGGCTCC GAGGGTGTCA GGAGCGGCTT CCCACTGCTG 
AAGGAGTTCG TCCAGAGAGT GGTGGAGAGC CTGGACGTGG GACCAGACCG GGTGCGTGTG 
GCCGTGGTGC AGTACAGCGA CCGGACCAGA CCTGAGTTCT ACCTGAATTC CTACATGGAC 
CAGCAGAGTG TGGTCGGAGC CATCCGTGGG CTGACCCTGC TGGGCGGGCC GGCCCCCAAC 
ACAGGGGCTG CCCTGGAGTT CGTCCTGAGG AACATCCTGG TGGGCTCTGC TGGGAGCCGC 
ATAGCAGAAG GTGTCCCCCA GCTCCTGATT GTCCTCACGG CCGACAGGTC TGGGGATGAC 
GTGAGGGGCC CCTCTGTGGT CCTGAGGAGG GGAGGGGCCG TGCCCATCGG CATCGGCATT 
GGGAACGCTG ACATCACCGA GATGCAGACC CTCTCCTTCG TCCCGGACTT CGCCGTGGTC 
ATCCCCACCT TCCGGCAGCT GGGGACCATC CAACAGGTCA TCTCGGAGAG AGTGACCCAG 
CTCAGCCGCG AGGAACTGAG CAGGCTGCAG GCGTCTGTCA CCCCCTTGAC GACTCCGGTG 
GTCAGCAGCA AGAGGGACGT GGTCTTTCTC ATCGATGGGT CCCAGAGTGC TGGCCCCGAG 
TTCCAGTACA TCCGAACGCT CATCGAGAGG CTGGTGGACT ACCTGGATGT GGGCTTCGAC 
ACGACCCGGG TGGCGGTCAT CCAGTTCAGC GATGACCCCA GGGTGGAGTT CCTGTTGAAC 
GTCCACTCCA GCAAGGACGA GGTGCAGAAC GCCGTGCGGC GGCTGCGGCC CAAAGGTGGG 
AGGCAGGTCA ACGTTGGGGG TGCCCTGGAG TACGTGGCCA GGAACATCTT CAAGAGGCCC 
CTGGGGAGCC GGATTGAGGA GGGTGTCCCT CAGTTCCTGG TCCTCATTTC CTCCGGGAAG 
TCGGATGATG AGGTGGAGGA CTCGGCCATA GAGCTCAAGC AGTTTGGCGT GGCGCCACTC 
ACGATCGCCA GGAACGTGGA CCAGGAAGAG CTGGTGAAGA TCTCGCTGAG CCCCGAGTAC 
GTGTTCTCAG TGAACACCTT CCGGGAGCTC CCCAGCCTGG AGCAGAAACT GCTGACACCG 
CTCACCACCC TGACTGCCGG GCAGATACAG CAGCTCCTGG CCAGCACGCG CTACCCGCCT 
CCAGCTGTGG AGAGCGATGC TGCGGACATT GTCTTCCTCA TCGACAGCTC TGATGGCGTG 
AAGCCGGATG GCATCGCACA CATCCGGGAC TTTGTTATCA GGATCGTGCG GAGACTCAAC 
GTCGGTCCCA ACAAAGTGAG AATTGGGGTC CTGCAGTTCA GCAACGACGT CTTCCCAGAA 
TTTCAACTAA AGACCTATAA ATCCCAGGCC AGCGTGCTGG ATGCCATACG TCGCCTGAGG 
TTCAAAGGGG GGTCTCCTCT CAACACTGGC AAGGCTCTGG AATTCGTGGC GAGGAATTAC 
TTTGTCAAGT CTGCCGGGAG CCGGATAGAA GATGGGGTGC CCCAACACCT GGTTCTATTT 
CTGGGTGGAA AATCCCAGGA TGATATTTCC AGGTATTCCC AGGTGATAAA ATCTGCAGGA 
ATTGCGAGCT TAGGGGTTGG AGACCGAAAT ATTGACAGAA CAGAGCTGCA GACCATCACC 
AGCGATCCCA GGCTTGTCTT CACGGTGCGG GAGTTCAGAG ACCTCCCCAG CATAGAAGAA 
AGGATGGTGA ATTCCTTTGG GTCCTCCGGG GTCACCCCTG CACCTCCGGG AGTGGACACA 
CCCTCTCCTT CAAGGCCAGA GAAGAAGAAA GCCGACATTG TGTTCCTGCT GGATGGTTCC 
ATCAATTTCC GGAGAGACAG TTTCCAGGAA GTGCTCCGTT TTGTGTCAGA AATTGTGGAC 
ACGGTTTATG AAGGGGGCGA CTCCATCCAG GTGGGGCTGG TCCAATACAA CTCTGACCCC 
ACCGACGAGT TCTTCCTGAA GGACTTCCCC ACCAAGCAGC AGATTATCGA CGCCATAAAC 
AAGGTGGTCT ACAAAGGGGG GAGGCACGCG AACACCAAGG TGGGCCTGGA GCACCTGCGG 
CGGAACCACT TCGTGCCCGA GGCGGGCAGC CGCCTGGACC AGAGGGTCCC GCAGATCGCC 
TTTGTGATCA CGGGGGGCAA GTCGGTGGAG GACGCTCAGG AGGCAAGCAT GGCGCTCACG 
CAGAGAGGCG TCAAGGTGTT TGCGGTGGGC GTAAGGAACA TCGACTCGGA GGAGGTTGGG 
AAGATAGCGT CCAACAGCGC CACGGCCTTC CGGGTGGGGA ACGTCCAGGA GCTGTCGGAA 
CTGAGCGAGC AGGTTTTGGA AACTCTGCAC GATGCAATGC ATGAGACCTT ATGTCCAGGC 
GTGACTGACG TTTCCAAAGC CTGTAACTTG GATGTGATTC TGGGCTTCGA TGGTTCAGGA 
GATCAGAATG TCTTTGTGGC TCAGAAGGGC CTCGAGCCCA AGGTGGACAC CATCCTGAGG 
AGAATCAGTC AGATGCAGAA GATCAGCTGC AGCGGCAGCC AGCTGCCCAC CGTGCGGGTG 
TCTGTGGTGG CCCTCACGCC CTCGGGCCCC GTTGAGGCCT TTGACTTTGC TGAGTACCAG 
CCGGAGCTGT TTGAGAAGTT CCAGAACATG CGTGCCCAGC ACCCCTACGT CCTCACTGCG 
GACACACTGA AGCTCTACCA GAACAAGTTC CGGCAGGCCT CGATGGACAA CGTGAAGGTG 
GTCATTCATT TTACCGATGG AGTGGATGGG GATCTGGCTG ATTTACAAAG AGCATCAGAG 
GAACTCCGAC AAGAAGGTGT CCGAGCTCTG ATCTTGGTGG GCCTCGAACG CGTCGCCAAC 
TTGGAGCAGC TCATGCAGCT GGAGTTCGGG CGAGGGTTCA CGTACAACCG GCCCCTGAGG 
CTGAACTTGC TGGACCTGGA CTACGAACTA GCCGAGCAGC TTGACAACAT TGCTGAGAAA 
GCCTGCTGTG GGATTCCCTG CAAATGCTCT GGACAAAGAG GAGACCGAGG GCCCATAGGC 
AGCATCGGGC CAAAGGGCAT TCCTGGAGAA GATGGCTACC GAGGTTATCC TGGTGACGAA 
GGTGGACCTG GTGAACGCGG TCCTCCTGGC GTGAACGGCA CTCAAGGTTT CCAGGGCTGC 
CCCGGGCAGA GAGGAGTCAA GGGCTCTCGT GGATTCCCGG GAGAGAAGGG TGAATTAGGA 
GAAATTGGAC TGGATGGTCT TGATGGTGAA GATGGCGACA AAGGGCTGCC CGGTACTTCT 
GGAGAGAAAG GGAGTCCAGG AAGGAGGGGC GACAAAGGAC CCAAAGGCGA CAAAGGGGAG 
AGAGGCGATG TCGGGATCAG AGGCGACCCG GGCAATTCAG GACAGGACAG CCAGCAGAGA 
GGAGCCAAAG GAGAAACCGG AGACATCGGC CCCGTGGGTC TCCCTGGGAG AGACGGGGTG 
TCTGGAGGGC CTGGAGAACC CGGGAAGAGC GGTGGCTTTG GCCGAAGGGG ACCAGCGGGA 
GCTAAGGGCA ACCAGGGCGG CCCCGGCCAG CAGGGCCCTG TAGGAGAGCA GGGGACCAGA 
GGCGCACAGG GTCCGCCTGG TCCCACCGGT CCTCCAGGCC TGATTGGTGA ACAGGGCATT 
CCCGGACCTC GGGGAGGCGG GGGAACCGCA GGTGCTCCTG GAGAACGCGG CAGAACCGGG 
CCCCTGGGAA GAAAGGGTGA GCCCGGAGAT CCAGGACCTA AGGGGAGCAT CGGGAACCGG 
GGTCCTCGTG GGGAGACGGG AGATGATGGG CGAGATGGAG TTGGCAGCGA AGGTCGCATA 
GGCAAGAAGG GAGAAAGAGG ATTCCCTGGA TACCCAGGTC CAAAGGGCAA TCCTGGTGAG 
CCAGGGACAG ACGGACCACC GGGACCCAAA GGCATCAGAG GCCGAAGGGG AAACTCGGGA 
CCTCCAGGGA TAGCTGGACA GAAGGGAGAC CCCGGTTACC CAGGGCCATC TGGTTACAAA 
GGCAGCAGAG GCGACTCAAT GGACCAATGT GCGCTGGTCC AGAGCATCAA AGATAAATGC 
CCTTGCTGTT ACGGGCCCCT GGAGTGCCCC GTCTTCCCGA CGGAGCTGGC TTTCGCCTTG 
GACACCTCAG AGGGGGTCAC CCAGGACAGG TTCAGCCAGA TGAGGGAGGT GGTCCTCAAG 
ATTCTGGACG ACCTGACCAT CGCCGAGAGC AACTGCCCGA GGGGGGCCCG CGTGGCCGTG 
GTCACTTACA ACAACGAGGT TACCACGGAG ATCCGCTTTG CCGACTCCAA GAAGAAGTCC 
GTCCTCCTGG ACAAGATCAA GAACCTGCAG GTGTCGCTGA CCTCCAAGCA GCAGAGCCTG 
GAAACCGCCA TGTCCTTTGT GGCCAGAAAC ACGTTTAAGC GTGTGCGGAG CGGGTTCCTG 
ATGAGGAAGG TGGCGGTGTT CTTCAGCAAC AAGCCCACGA GGGCGACCCC GCAGCTCCGG 
GAGGCTGTGC TCAAGCTCTC AGATGCTGGG ATCACACCCT TGTTCCTCAC AAGCCAGGAG 
GACCGGCAGC TGATCAATGC CCTACAGATC AATAACACAG CGGTGGGACA TGCCCTTGTC 
CTTCCTGCTG GCGGAGACCT CACAGACTTC TTGAAGAATG TCCTGACGTG TCACGTCTGC 
TTGGACATCT GCAACATTGA CCCATCCTGT GGATTCGGCA GCTGGAGGCC TTCCTTCAGG 
GACCGGAGGG CAGCAGGCAG TGATGTGGAC ATCGACATGG CTTTCATCTT AGATAGCTCC 
GAGTCCACCA CCCCGTTCCA GTTCAACGAG ATGAGGAAGT ACATCGAGTA CCTGGTCCGA 
CAGCTGGATG TGAGCCCAGA CCCCAAGGCC TCCCAGCACT TTGCCCGCGT GGCGGTGGTG 
CAGCATGCGC CCTACGAGGC GGTGGACAAC GCCAGTGTGC CGCCTGTGAA GGTGGAGTTC 
TCCCTGACCG ACTACGGCTC CAAAGAGAAG CTGCTGGCCT TCCTTGGCAG CAGGATGACC 
CAGCTGCAGG GGACCAGGGC TTTGGGCCGC GCCATCGACT ACACCATTGA GAACATCTTC 
GAAAGTGCTC CCAACCCCCG GGACCTGAAG ATCGTGGTGC TGATGCTGAC GGGCGAGGTG 
CAGAAGCAGC AGCTGGAGGA GGCCCAGAGA GCCATCCTAC AGGCCAAGTG CAAAGGCTAC 
TTCTTCGTGA TCCTGGGCAT TGGCAGGAAG GTGAATGTCA AGGAGCTGTA CAGCTCTGCC 
AGCGAGCCCA ACGACGTCTT CTTCAAGCTA GTGGACAAGT CGACCGAGCT CAACGAGGAG 
CCCCTAATGC GCTTCGGGAG GCTGCTGCCA TCCTTCGTCA GCAGTAAAGA TGCTTTCTAC 
CTGTCCCCAG ATATCAGGAA ACAGTGTGAT TGGTTCCAAG CAGACCAACC GGCAAAGAAT 
CTCGTGCAGT TTGGTTACAA ACAAATAAAT GTTCCTAATA ACGTTACTTC AAGTCCTACA 
TCTAAACTGG TGACCACAAC GAAGCCGGTG ATCACCACCA CCAAACCGAT CACAGTGGTG 
AACCTGCCAG CCTCAAAGCC AGCTGCCACG AGACCTGTGG ATGAGAGACC CGCAGCTGGG 
AGATCCGTGG CCACCAAGAT GGACAGTGCC AGGCCCGTGG CCACCAAGAC CGAGGCCACC 
AAACCCGTGG CCACCAAGAC CGAGGCCACC AAGACTGAAG CTGCCAAACC TGTGGTCACC 
AAGGCTGAGG CTGCAAAACC CACGGCCACC AAGACTGAAG CCACTAAGAC CGAGGCTGCA 
AAGCTCATGG CCACCAAGAC CGAGGCCACC AAACCTGTGG CTGCCAGGCC CGAGGCGGCA 
AAGACAGCCA CAGCCAGACC TGCAGCAGCC GCGAAGCCTG CAGCAGCCAA ACCTGCCCCT 
GCCAGACCCC CCACTGCTGC CAGATCCATG GCCACGAGGT CCGAGGCCCC CAGGCCCCAG 
GCAGTCAAAC TGGCTGCCAG CAGGCCCGGC GCTGCTAAGC CCGTGGTGAA GGCACCTCGC 
GAGATCCATG TGTCTGAGGT CACAGAGAAC AGCGCCAAGC TGCACTGGGA GAGGCCCGAG 
CCCCCCAGCC CTTACTTATA CAACCTCACC GTCACCTCGG CCCACGATCA GGCCGTGGTT 
CTGAAGCAGA ACCTCACTGT CACGGACCGC GTCATCGGGG GCCTGCTCCC CGGGCAGACT 
TACCACGTTA CGGTGACCTG CTCCCTGAGG TCTCAGGTCA GAGCCATCTA CCAAGGCAGC 
TTCAGCACGA AGAAAATTCA GCCTCCACCT CCACAAACAG AGCGATCAGC CTCTAGTGCA 
ACCATCAACC TAGTGGTGAG CGCAGACCGG TTGGCTGGGA GTAAAGCAGA TATATGTAAG 
TTGCCAAAGG ACGGCGGAAC TTGCAGAGAG TTTGTATTAA AGTGGTACTA TGATTCCGTA 
ACTGAAAACT GCGCCCGATT CTGGTATGGA GGCTGCGGTG GAAACGAAAA CAGATTTAAT 
TCGCAGGATG AATGTGAAAA GGTTTGCCCT CCTGTGCCCA TCAAGCAGCC CGGAGTTATC 
GCCGCGATGG GGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_005197965.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005198022.1 Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X4, mRNA.

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CloneID	OBa03699
Clone ID Related Accession (Same CDS sequence)	XM_005197965.3 , XM_024990265.1 , XM_005204980.3
Accession Version	XM_005197965.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644673.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197965.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC 
CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT 
GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG 
TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC 
ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT 
CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG 
GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC 
GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC 
CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC 
CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT 
GGAACCACTG AAGTTCGCGT AAACAAAAGG GATATCATCT TTCTTTTGGA TGGATCGTCC 
AACGTCGGAG AGACCAATTT CCCTTATGTG CGGGACTTTG TCATGAACCT AGTTAACAGC 
CTTGATGTTG GAAGCGACCA CATTCGGGTC GGTCTAGTGC AATTTAGTGA CACTCCGGTG 
ACGGAGTTCT CCCTAAACAC CTACCCGACC AAGTCAGAGT TGCTAGCTCA CCTGAGGCAG 
ATGCAGCTCC AGGGGGGGTC GGTCCTGAAC ACGGGCGCGG CCCTGAGCTA TGTGCATGCC 
AACCACTTCA CCGAAGCTGG CGGCAGCAGG ATCCAGGACC ACGTGCCGCA GCTGCTACTC 
CTGCTCACGG CCGGGCAGTC TGAGGACTCC TATTTGCAAG CCGCCAACGC CCTGGCGCGC 
GCGGGCATCC TGACCTTTTG TGTGGGCACT AGCCAGGCCG ATAGGGCGGA GCTTGAGGAG 
ATTGCTTTTA ACCCGGGCCT GGTGTATCTC ATGGATGATT TCAGCTCCTT GCCAGCTTTG 
CCTCAACAGC TGATTCAGCC CCTAACCACT TATGTTAGCG GAGGTGTGGA GGAGGTGCCT 
CTTGCCCAGC CAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC 
ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG 
AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC 
CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC 
AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT 
GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG 
GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG 
GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA 
TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG 
ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG 
GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG 
GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC 
GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG 
CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA 
GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA 
GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG 
AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG 
AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC 
CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC 
AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC 
AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC 
CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG 
ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC 
CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG 
CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG 
GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG 
GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG 
ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG 
TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC 
ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA 
GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG 
CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC 
GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT 
CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC 
AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT 
GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG 
GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT 
GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC 
GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG 
ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC 
TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC 
AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG 
GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC 
GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG 
GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG 
AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT 
GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG 
AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG 
ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG 
AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG 
ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT 
CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA 
ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT 
GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG 
GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC 
ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC 
ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA 
CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG 
GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG 
AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC 
TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC 
ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT 
GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC 
GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA 
ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA 
GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA 
GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA 
GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT 
GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT 
AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC 
GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC 
GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC 
CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT 
CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC 
AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA 
GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT 
CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC 
AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT 
TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC 
ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT 
CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC 
ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC 
CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA 
ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG 
AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG 
GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC 
CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT 
CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG 
GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC 
CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG 
TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG 
CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG 
CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC 
CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG 
CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA 
AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG 
AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC 
TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC 
GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC 
CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG 
TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC 
GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT 
AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC 
CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA 
TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA 
CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG 
GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG 
CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG 
ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC 
AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA 
GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG 
ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC 
CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG 
AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC 
CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC 
AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC 
ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG 
CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT 
GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG 
CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC 
GCGATGGGGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005198022.1
CDS	343..8817
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEVRVNKRDIIFLLDGSSNVGETNFPYVRDFVMNLVNSLDVGSDHIRVGLVQFSDTPVTEFSLNTYPTKSELLAHLRQMQLQGGSVLNTGAALSYVHANHFTEAGGSRIQDHVPQLLLLLTAGQSEDSYLQAANALARAGILTFCVGTSQADRAELEEIAFNPGLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_005197965.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197965.3

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC 
CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT 
GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG 
TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC 
ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT 
CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG 
GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC 
GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC 
CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC 
CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT 
GGAACCACTG AAGTTCGCGT AAACAAAAGG GATATCATCT TTCTTTTGGA TGGATCGTCC 
AACGTCGGAG AGACCAATTT CCCTTATGTG CGGGACTTTG TCATGAACCT AGTTAACAGC 
CTTGATGTTG GAAGCGACCA CATTCGGGTC GGTCTAGTGC AATTTAGTGA CACTCCGGTG 
ACGGAGTTCT CCCTAAACAC CTACCCGACC AAGTCAGAGT TGCTAGCTCA CCTGAGGCAG 
ATGCAGCTCC AGGGGGGGTC GGTCCTGAAC ACGGGCGCGG CCCTGAGCTA TGTGCATGCC 
AACCACTTCA CCGAAGCTGG CGGCAGCAGG ATCCAGGACC ACGTGCCGCA GCTGCTACTC 
CTGCTCACGG CCGGGCAGTC TGAGGACTCC TATTTGCAAG CCGCCAACGC CCTGGCGCGC 
GCGGGCATCC TGACCTTTTG TGTGGGCACT AGCCAGGCCG ATAGGGCGGA GCTTGAGGAG 
ATTGCTTTTA ACCCGGGCCT GGTGTATCTC ATGGATGATT TCAGCTCCTT GCCAGCTTTG 
CCTCAACAGC TGATTCAGCC CCTAACCACT TATGTTAGCG GAGGTGTGGA GGAGGTGCCT 
CTTGCCCAGC CAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC 
ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG 
AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC 
CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC 
AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT 
GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG 
GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG 
GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA 
TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG 
ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG 
GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG 
GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC 
GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG 
CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA 
GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA 
GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG 
AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG 
AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC 
CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC 
AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC 
AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC 
CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG 
ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC 
CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG 
CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG 
GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG 
GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG 
ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG 
TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC 
ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA 
GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG 
CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC 
GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT 
CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC 
AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT 
GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG 
GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT 
GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC 
GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG 
ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC 
TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC 
AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG 
GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC 
GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG 
GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG 
AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT 
GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG 
AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG 
ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG 
AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG 
ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT 
CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA 
ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT 
GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG 
GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC 
ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC 
ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA 
CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG 
GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG 
AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC 
TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC 
ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT 
GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC 
GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA 
ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA 
GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA 
GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA 
GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT 
GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT 
AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC 
GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC 
GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC 
CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT 
CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC 
AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA 
GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT 
CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC 
AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT 
TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC 
ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT 
CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC 
ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC 
CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA 
ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG 
AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG 
GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC 
CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT 
CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG 
GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC 
CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG 
TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG 
CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG 
CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC 
CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG 
CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA 
AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG 
AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC 
TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC 
GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC 
CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG 
TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC 
GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT 
AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC 
CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA 
TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA 
CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG 
GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG 
CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG 
ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC 
AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA 
GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG 
ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC 
CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG 
AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC 
CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC 
AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC 
ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG 
CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT 
GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG 
CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC 
GCGATGGGGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_024990265.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846033.1 Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X4, mRNA.

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CloneID	OBa03699
Clone ID Related Accession (Same CDS sequence)	XM_005197965.3 , XM_024990265.1 , XM_005204980.3
Accession Version	XM_024990265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC 
CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT 
GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG 
TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC 
ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT 
CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG 
GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC 
GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC 
CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC 
CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT 
GGAACCACTG AAGTTCGCGT AAACAAAAGG GATATCATCT TTCTTTTGGA TGGATCGTCC 
AACGTCGGAG AGACCAATTT CCCTTATGTG CGGGACTTTG TCATGAACCT AGTTAACAGC 
CTTGATGTTG GAAGCGACCA CATTCGGGTC GGTCTAGTGC AATTTAGTGA CACTCCGGTG 
ACGGAGTTCT CCCTAAACAC CTACCCGACC AAGTCAGAGT TGCTAGCTCA CCTGAGGCAG 
ATGCAGCTCC AGGGGGGGTC GGTCCTGAAC ACGGGCGCGG CCCTGAGCTA TGTGCATGCC 
AACCACTTCA CCGAAGCTGG CGGCAGCAGG ATCCAGGACC ACGTGCCGCA GCTGCTACTC 
CTGCTCACGG CCGGGCAGTC TGAGGACTCC TATTTGCAAG CCGCCAACGC CCTGGCGCGC 
GCGGGCATCC TGACCTTTTG TGTGGGCACT AGCCAGGCCG ATAGGGCGGA GCTTGAGGAG 
ATTGCTTTTA ACCCGGGCCT GGTGTATCTC ATGGATGATT TCAGCTCCTT GCCAGCTTTG 
CCTCAACAGC TGATTCAGCC CCTAACCACT TATGTTAGCG GAGGTGTGGA GGAGGTGCCT 
CTTGCCCAGC CAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC 
ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG 
AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC 
CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC 
AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT 
GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG 
GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG 
GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA 
TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG 
ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG 
GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG 
GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC 
GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG 
CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA 
GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA 
GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG 
AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG 
AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC 
CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC 
AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC 
AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC 
CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG 
ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC 
CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG 
CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG 
GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG 
GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG 
ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG 
TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC 
ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA 
GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG 
CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC 
GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT 
CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC 
AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT 
GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG 
GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT 
GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC 
GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG 
ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC 
TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC 
AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG 
GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC 
GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG 
GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG 
AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT 
GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG 
AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG 
ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG 
AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG 
ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT 
CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA 
ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT 
GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG 
GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC 
ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC 
ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA 
CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG 
GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG 
AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC 
TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC 
ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT 
GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC 
GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA 
ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA 
GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA 
GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA 
GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT 
GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT 
AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC 
GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC 
GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC 
CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT 
CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC 
AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA 
GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT 
CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC 
AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT 
TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC 
ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT 
CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC 
ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC 
CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA 
ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG 
AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG 
GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC 
CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT 
CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG 
GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC 
CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG 
TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG 
CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG 
CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC 
CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG 
CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA 
AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG 
AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC 
TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC 
GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC 
CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG 
TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC 
GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT 
AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC 
CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA 
TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA 
CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG 
GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG 
CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG 
ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC 
AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA 
GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG 
ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC 
CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG 
AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC 
CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC 
AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC 
ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG 
CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT 
GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG 
CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC 
GCGATGGGGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846033.1
CDS	276..8750
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEVRVNKRDIIFLLDGSSNVGETNFPYVRDFVMNLVNSLDVGSDHIRVGLVQFSDTPVTEFSLNTYPTKSELLAHLRQMQLQGGSVLNTGAALSYVHANHFTEAGGSRIQDHVPQLLLLLTAGQSEDSYLQAANALARAGILTFCVGTSQADRAELEEIAFNPGLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_024990265.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990265.1

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC 
CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT 
GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG 
TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC 
ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT 
CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG 
GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC 
GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC 
CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC 
CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT 
GGAACCACTG AAGTTCGCGT AAACAAAAGG GATATCATCT TTCTTTTGGA TGGATCGTCC 
AACGTCGGAG AGACCAATTT CCCTTATGTG CGGGACTTTG TCATGAACCT AGTTAACAGC 
CTTGATGTTG GAAGCGACCA CATTCGGGTC GGTCTAGTGC AATTTAGTGA CACTCCGGTG 
ACGGAGTTCT CCCTAAACAC CTACCCGACC AAGTCAGAGT TGCTAGCTCA CCTGAGGCAG 
ATGCAGCTCC AGGGGGGGTC GGTCCTGAAC ACGGGCGCGG CCCTGAGCTA TGTGCATGCC 
AACCACTTCA CCGAAGCTGG CGGCAGCAGG ATCCAGGACC ACGTGCCGCA GCTGCTACTC 
CTGCTCACGG CCGGGCAGTC TGAGGACTCC TATTTGCAAG CCGCCAACGC CCTGGCGCGC 
GCGGGCATCC TGACCTTTTG TGTGGGCACT AGCCAGGCCG ATAGGGCGGA GCTTGAGGAG 
ATTGCTTTTA ACCCGGGCCT GGTGTATCTC ATGGATGATT TCAGCTCCTT GCCAGCTTTG 
CCTCAACAGC TGATTCAGCC CCTAACCACT TATGTTAGCG GAGGTGTGGA GGAGGTGCCT 
CTTGCCCAGC CAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC 
ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG 
AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC 
CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC 
AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT 
GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG 
GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG 
GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA 
TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG 
ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG 
GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG 
GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC 
GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG 
CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA 
GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA 
GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG 
AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG 
AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC 
CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC 
AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC 
AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC 
CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG 
ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC 
CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG 
CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG 
GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG 
GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG 
ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG 
TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC 
ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA 
GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG 
CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC 
GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT 
CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC 
AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT 
GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG 
GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT 
GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC 
GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG 
ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC 
TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC 
AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG 
GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC 
GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG 
GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG 
AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT 
GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG 
AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG 
ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG 
AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG 
ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT 
CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA 
ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT 
GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG 
GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC 
ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC 
ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA 
CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG 
GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG 
AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC 
TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC 
ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT 
GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC 
GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA 
ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA 
GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA 
GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA 
GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT 
GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT 
AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC 
GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC 
GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC 
CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT 
CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC 
AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA 
GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT 
CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC 
AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT 
TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC 
ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT 
CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC 
ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC 
CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA 
ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG 
AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG 
GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC 
CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT 
CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG 
GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC 
CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG 
TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG 
CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG 
CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC 
CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG 
CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA 
AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG 
AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC 
TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC 
GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC 
CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG 
TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC 
GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT 
AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC 
CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA 
TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA 
CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG 
GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG 
CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG 
ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC 
AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA 
GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG 
ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC 
CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG 
AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC 
CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC 
AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC 
ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG 
CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT 
GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG 
CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC 
GCGATGGGGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_005204980.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205037.1 Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X4, mRNA.

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CloneID	OBa03699
Clone ID Related Accession (Same CDS sequence)	XM_005197965.3 , XM_024990265.1 , XM_005204980.3
Accession Version	XM_005204980.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC 
CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT 
GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG 
TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC 
ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT 
CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG 
GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC 
GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC 
CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC 
CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT 
GGAACCACTG AAGTTCGCGT AAACAAAAGG GATATCATCT TTCTTTTGGA TGGATCGTCC 
AACGTCGGAG AGACCAATTT CCCTTATGTG CGGGACTTTG TCATGAACCT AGTTAACAGC 
CTTGATGTTG GAAGCGACCA CATTCGGGTC GGTCTAGTGC AATTTAGTGA CACTCCGGTG 
ACGGAGTTCT CCCTAAACAC CTACCCGACC AAGTCAGAGT TGCTAGCTCA CCTGAGGCAG 
ATGCAGCTCC AGGGGGGGTC GGTCCTGAAC ACGGGCGCGG CCCTGAGCTA TGTGCATGCC 
AACCACTTCA CCGAAGCTGG CGGCAGCAGG ATCCAGGACC ACGTGCCGCA GCTGCTACTC 
CTGCTCACGG CCGGGCAGTC TGAGGACTCC TATTTGCAAG CCGCCAACGC CCTGGCGCGC 
GCGGGCATCC TGACCTTTTG TGTGGGCACT AGCCAGGCCG ATAGGGCGGA GCTTGAGGAG 
ATTGCTTTTA ACCCGGGCCT GGTGTATCTC ATGGATGATT TCAGCTCCTT GCCAGCTTTG 
CCTCAACAGC TGATTCAGCC CCTAACCACT TATGTTAGCG GAGGTGTGGA GGAGGTGCCT 
CTTGCCCAGC CAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC 
ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG 
AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC 
CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC 
AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT 
GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG 
GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG 
GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA 
TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG 
ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG 
GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG 
GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC 
GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG 
CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA 
GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA 
GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG 
AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG 
AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC 
CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC 
AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC 
AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC 
CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG 
ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC 
CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG 
CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG 
GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG 
GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG 
ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG 
TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC 
ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA 
GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG 
CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC 
GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT 
CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC 
AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT 
GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG 
GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT 
GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC 
GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG 
ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC 
TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC 
AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG 
GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC 
GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG 
GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG 
AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT 
GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG 
AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG 
ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG 
AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG 
ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT 
CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA 
ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT 
GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG 
GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC 
ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC 
ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA 
CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG 
GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG 
AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC 
TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC 
ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT 
GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC 
GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA 
ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA 
GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA 
GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA 
GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT 
GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT 
AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC 
GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC 
GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC 
CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT 
CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC 
AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA 
GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT 
CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC 
AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT 
TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC 
ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT 
CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC 
ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC 
CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA 
ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG 
AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG 
GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC 
CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT 
CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG 
GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC 
CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG 
TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG 
CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG 
CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC 
CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG 
CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA 
AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG 
AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC 
TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC 
GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC 
CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG 
TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC 
GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT 
AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC 
CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA 
TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA 
CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG 
GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG 
CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG 
ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC 
AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA 
GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG 
ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC 
CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG 
AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC 
CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC 
AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC 
ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG 
CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT 
GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG 
CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC 
GCGATGGGGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205037.1
CDS	343..8817
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEVRVNKRDIIFLLDGSSNVGETNFPYVRDFVMNLVNSLDVGSDHIRVGLVQFSDTPVTEFSLNTYPTKSELLAHLRQMQLQGGSVLNTGAALSYVHANHFTEAGGSRIQDHVPQLLLLLTAGQSEDSYLQAANALARAGILTFCVGTSQADRAELEEIAFNPGLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_005204980.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204980.3

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC 
CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT 
GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG 
TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC 
ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT 
CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG 
GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC 
GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC 
CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC 
CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT 
GGAACCACTG AAGTTCGCGT AAACAAAAGG GATATCATCT TTCTTTTGGA TGGATCGTCC 
AACGTCGGAG AGACCAATTT CCCTTATGTG CGGGACTTTG TCATGAACCT AGTTAACAGC 
CTTGATGTTG GAAGCGACCA CATTCGGGTC GGTCTAGTGC AATTTAGTGA CACTCCGGTG 
ACGGAGTTCT CCCTAAACAC CTACCCGACC AAGTCAGAGT TGCTAGCTCA CCTGAGGCAG 
ATGCAGCTCC AGGGGGGGTC GGTCCTGAAC ACGGGCGCGG CCCTGAGCTA TGTGCATGCC 
AACCACTTCA CCGAAGCTGG CGGCAGCAGG ATCCAGGACC ACGTGCCGCA GCTGCTACTC 
CTGCTCACGG CCGGGCAGTC TGAGGACTCC TATTTGCAAG CCGCCAACGC CCTGGCGCGC 
GCGGGCATCC TGACCTTTTG TGTGGGCACT AGCCAGGCCG ATAGGGCGGA GCTTGAGGAG 
ATTGCTTTTA ACCCGGGCCT GGTGTATCTC ATGGATGATT TCAGCTCCTT GCCAGCTTTG 
CCTCAACAGC TGATTCAGCC CCTAACCACT TATGTTAGCG GAGGTGTGGA GGAGGTGCCT 
CTTGCCCAGC CAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC 
ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG 
AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC 
CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC 
AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT 
GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG 
GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG 
GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA 
TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG 
ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG 
GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG 
GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC 
GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG 
CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA 
GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA 
GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG 
AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG 
AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC 
CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC 
AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC 
AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC 
CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG 
ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC 
CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG 
CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG 
GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG 
GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG 
ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG 
TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC 
ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA 
GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG 
CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC 
GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT 
CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC 
AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT 
GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG 
GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT 
GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC 
GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG 
ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC 
TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC 
AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG 
GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC 
GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG 
GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG 
AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT 
GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG 
AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG 
ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG 
AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG 
ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT 
CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA 
ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT 
GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG 
GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC 
ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC 
ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA 
CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG 
GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG 
AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC 
TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC 
ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT 
GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC 
GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA 
ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA 
GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA 
GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA 
GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT 
GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT 
AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC 
GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC 
GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC 
CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT 
CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC 
AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA 
GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT 
CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC 
AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT 
TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC 
ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT 
CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC 
ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC 
CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA 
ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG 
AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG 
GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC 
CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT 
CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG 
GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC 
CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG 
TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG 
CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG 
CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC 
CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG 
CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA 
AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG 
AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC 
TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC 
GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC 
CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG 
TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC 
GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT 
AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC 
CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA 
TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA 
CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG 
GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG 
CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG 
ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC 
AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA 
GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG 
ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC 
CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG 
AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC 
CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC 
AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC 
ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG 
CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT 
GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG 
CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC 
GCGATGGGGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_024990262.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846030.1 Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X1, mRNA.

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CloneID	OBa233420
Clone ID Related Accession (Same CDS sequence)	XM_024990262.1
Accession Version	XM_024990262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA 
TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT 
CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC 
CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA 
GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA 
TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA 
GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT 
GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG 
AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG 
GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT 
GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT 
CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG 
GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC 
AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT 
GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA 
GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATCCGAGACG GGGTCGTAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC 
CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC 
GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC 
AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC 
AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT 
ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC 
TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA 
GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC 
GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC 
CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC 
GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG 
GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC 
GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGTTCGCG TAAACAAAAG GGATATCATC 
TTTCTTTTGG ATGGATCGTC CAACGTCGGA GAGACCAATT TCCCTTATGT GCGGGACTTT 
GTCATGAACC TAGTTAACAG CCTTGATGTT GGAAGCGACC ACATTCGGGT CGGTCTAGTG 
CAATTTAGTG ACACTCCGGT GACGGAGTTC TCCCTAAACA CCTACCCGAC CAAGTCAGAG 
TTGCTAGCTC ACCTGAGGCA GATGCAGCTC CAGGGGGGGT CGGTCCTGAA CACGGGCGCG 
GCCCTGAGCT ATGTGCATGC CAACCACTTC ACCGAAGCTG GCGGCAGCAG GATCCAGGAC 
CACGTGCCGC AGCTGCTACT CCTGCTCACG GCCGGGCAGT CTGAGGACTC CTATTTGCAA 
GCCGCCAACG CCCTGGCGCG CGCGGGCATC CTGACCTTTT GTGTGGGCAC TAGCCAGGCC 
GATAGGGCGG AGCTTGAGGA GATTGCTTTT AACCCGGGCC TGGTGTATCT CATGGATGAT 
TTCAGCTCCT TGCCAGCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC TTATGTTAGC 
GGAGGTGTGG AGGAGGTGCC TCTTGCCCAG CCAGAGAGCA AGCGAGACAT CCTGTTCCTT 
TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG 
GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT 
GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT 
CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT 
TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT 
CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC 
AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT 
GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG 
ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG 
GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG 
AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA 
CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC 
CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG 
GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG 
GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC 
GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG 
CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC 
CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC 
TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC 
CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC 
CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC 
CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG 
GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG 
CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG 
AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC 
CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG 
TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC 
TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG 
CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC 
AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC 
GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG 
ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC 
AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT 
GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA 
TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC 
CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG 
GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA 
GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC 
CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA 
CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG 
TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT 
GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC 
CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG 
ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG 
GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG 
AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG 
GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC 
GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC 
GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT 
GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG 
GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG 
GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG 
CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT 
GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC 
CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG 
ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT 
TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC 
CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG 
TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT 
GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA 
GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA 
GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT 
CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA 
GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA 
GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC 
AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA 
CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC 
CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC 
CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA 
GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG 
ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA 
GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG 
GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT 
GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA 
AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC 
CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA 
GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG 
AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG 
GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG 
AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG 
GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC 
GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC 
TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT 
GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG 
GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG 
TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG 
GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC 
CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC 
TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT 
TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC 
ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT 
GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG 
CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC 
CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC 
ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG 
ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG 
GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG 
GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG 
ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC 
AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA 
GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC 
GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC 
AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT 
GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC 
ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT 
GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC 
ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT 
GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA 
GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC 
GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC 
GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG 
CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC 
CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC 
CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA 
GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG 
CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT 
AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG 
TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA 
AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC 
AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846030.1
CDS	278..9973
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPDVKNGAAADIIFLVDSSWSIGKEHFQLVREFLYDVIESLAVGDNDFRFALVQFNGNPHTEFLFNTYRSKQEVLSHVSNMSYIGGSNQTGKGLAYVMQNHLTEAAGSRASDGVPQVIVVLTHGHSEDGLALPSAELKSADVNVFAIGVEDADEAALKEIASEPLNMHVFNLENYTSLHDIVGNLVACVRSSMAPERAGGTEIPKDITAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLERLSIGTQQIRVGVVQYSDEPRTMFSLNSYSTKAQVLDAVKALGFIGGELANVGLALDFVVENHFTRAGGSRAEEGVPQVLVLISAGPSSDEIRDGVVALKQASVFSFGLGAQAASKAELQHIATNDNLVFTVPEFRSLGDVQEQLLPYIVGVAQRHIVLQPPTIVTQVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEVRVNKRDIIFLLDGSSNVGETNFPYVRDFVMNLVNSLDVGSDHIRVGLVQFSDTPVTEFSLNTYPTKSELLAHLRQMQLQGGSVLNTGAALSYVHANHFTEAGGSRIQDHVPQLLLLLTAGQSEDSYLQAANALARAGILTFCVGTSQADRAELEEIAFNPGLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_024990262.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990262.1

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA 
TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT 
CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC 
CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA 
GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA 
TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA 
GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT 
GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG 
AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG 
GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT 
GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT 
CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG 
GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC 
AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT 
GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA 
GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATCCGAGACG GGGTCGTAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC 
CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC 
GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC 
AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC 
AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT 
ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC 
TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA 
GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC 
GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC 
CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC 
GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG 
GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC 
GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGTTCGCG TAAACAAAAG GGATATCATC 
TTTCTTTTGG ATGGATCGTC CAACGTCGGA GAGACCAATT TCCCTTATGT GCGGGACTTT 
GTCATGAACC TAGTTAACAG CCTTGATGTT GGAAGCGACC ACATTCGGGT CGGTCTAGTG 
CAATTTAGTG ACACTCCGGT GACGGAGTTC TCCCTAAACA CCTACCCGAC CAAGTCAGAG 
TTGCTAGCTC ACCTGAGGCA GATGCAGCTC CAGGGGGGGT CGGTCCTGAA CACGGGCGCG 
GCCCTGAGCT ATGTGCATGC CAACCACTTC ACCGAAGCTG GCGGCAGCAG GATCCAGGAC 
CACGTGCCGC AGCTGCTACT CCTGCTCACG GCCGGGCAGT CTGAGGACTC CTATTTGCAA 
GCCGCCAACG CCCTGGCGCG CGCGGGCATC CTGACCTTTT GTGTGGGCAC TAGCCAGGCC 
GATAGGGCGG AGCTTGAGGA GATTGCTTTT AACCCGGGCC TGGTGTATCT CATGGATGAT 
TTCAGCTCCT TGCCAGCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC TTATGTTAGC 
GGAGGTGTGG AGGAGGTGCC TCTTGCCCAG CCAGAGAGCA AGCGAGACAT CCTGTTCCTT 
TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG 
GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT 
GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT 
CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT 
TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT 
CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC 
AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT 
GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG 
ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG 
GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG 
AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA 
CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC 
CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG 
GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG 
GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC 
GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG 
CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC 
CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC 
TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC 
CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC 
CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC 
CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG 
GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG 
CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG 
AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC 
CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG 
TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC 
TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG 
CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC 
AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC 
GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG 
ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC 
AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT 
GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA 
TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC 
CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG 
GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA 
GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC 
CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA 
CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG 
TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT 
GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC 
CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG 
ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG 
GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG 
AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG 
GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC 
GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC 
GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT 
GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG 
GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG 
GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG 
CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT 
GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC 
CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG 
ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT 
TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC 
CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG 
TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT 
GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA 
GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA 
GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT 
CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA 
GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA 
GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC 
AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA 
CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC 
CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC 
CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA 
GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG 
ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA 
GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG 
GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT 
GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA 
AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC 
CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA 
GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG 
AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG 
GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG 
AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG 
GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC 
GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC 
TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT 
GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG 
GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG 
TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG 
GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC 
CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC 
TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT 
TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC 
ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT 
GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG 
CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC 
CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC 
ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG 
ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG 
GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG 
GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG 
ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC 
AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA 
GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC 
GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC 
AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT 
GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC 
ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT 
GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC 
ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT 
GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA 
GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC 
GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC 
GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG 
CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC 
CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC 
CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA 
GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG 
CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT 
AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG 
TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA 
AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC 
AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_024990263.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846031.1 Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X2, mRNA.

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CloneID	OBa233421
Clone ID Related Accession (Same CDS sequence)	XM_024990263.1
Accession Version	XM_024990263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA 
TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT 
CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC 
CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA 
GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA 
TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA 
GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT 
GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG 
AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG 
GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT 
GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT 
CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG 
GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC 
AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT 
GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA 
GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATCCGAGACG GGGTCGTAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC 
CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC 
GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC 
AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC 
AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT 
ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC 
TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA 
GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC 
GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC 
CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC 
GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG 
GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC 
GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGAGAGCA AGCGAGACAT CCTGTTCCTT 
TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG 
GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT 
GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT 
CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT 
TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT 
CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC 
AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT 
GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG 
ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG 
GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG 
AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA 
CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC 
CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG 
GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG 
GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC 
GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG 
CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC 
CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC 
TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC 
CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC 
CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC 
CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG 
GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG 
CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG 
AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC 
CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG 
TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC 
TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG 
CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC 
AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC 
GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG 
ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC 
AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT 
GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA 
TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC 
CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG 
GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA 
GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC 
CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA 
CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG 
TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT 
GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC 
CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG 
ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG 
GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG 
AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG 
GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC 
GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC 
GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT 
GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG 
GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG 
GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG 
CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT 
GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC 
CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG 
ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT 
TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC 
CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG 
TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT 
GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA 
GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA 
GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT 
CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA 
GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA 
GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC 
AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA 
CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC 
CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC 
CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA 
GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG 
ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA 
GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG 
GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT 
GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA 
AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC 
CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA 
GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG 
AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG 
GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG 
AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG 
GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC 
GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC 
TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT 
GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG 
GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG 
TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG 
GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC 
CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC 
TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT 
TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC 
ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT 
GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG 
CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC 
CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC 
ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG 
ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG 
GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG 
GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG 
ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC 
AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA 
GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC 
GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC 
AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT 
GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC 
ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT 
GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC 
ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT 
GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA 
GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC 
GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC 
GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG 
CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC 
CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC 
CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA 
GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG 
CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT 
AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG 
TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA 
AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC 
AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846031.1
CDS	277..9372
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPDVKNGAAADIIFLVDSSWSIGKEHFQLVREFLYDVIESLAVGDNDFRFALVQFNGNPHTEFLFNTYRSKQEVLSHVSNMSYIGGSNQTGKGLAYVMQNHLTEAAGSRASDGVPQVIVVLTHGHSEDGLALPSAELKSADVNVFAIGVEDADEAALKEIASEPLNMHVFNLENYTSLHDIVGNLVACVRSSMAPERAGGTEIPKDITAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLERLSIGTQQIRVGVVQYSDEPRTMFSLNSYSTKAQVLDAVKALGFIGGELANVGLALDFVVENHFTRAGGSRAEEGVPQVLVLISAGPSSDEIRDGVVALKQASVFSFGLGAQAASKAELQHIATNDNLVFTVPEFRSLGDVQEQLLPYIVGVAQRHIVLQPPTIVTQVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_024990263.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990263.1

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA 
TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT 
CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC 
CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA 
GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA 
TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA 
GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT 
GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG 
AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG 
GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT 
GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT 
CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG 
GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC 
AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT 
GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA 
GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATCCGAGACG GGGTCGTAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC 
CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC 
GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC 
AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC 
AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT 
ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC 
TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA 
GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC 
GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC 
CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC 
GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG 
GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC 
GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGAGAGCA AGCGAGACAT CCTGTTCCTT 
TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG 
GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT 
GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT 
CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT 
TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT 
CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC 
AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT 
GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG 
ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG 
GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG 
AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA 
CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC 
CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG 
GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG 
GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC 
GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG 
CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC 
CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC 
TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC 
CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC 
CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC 
CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG 
GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG 
CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG 
AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC 
CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG 
TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC 
TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG 
CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC 
AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC 
GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG 
ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC 
AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT 
GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA 
TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC 
CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG 
GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA 
GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC 
CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA 
CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG 
TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT 
GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC 
CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG 
ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG 
GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG 
AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG 
GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC 
GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC 
GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT 
GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG 
GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG 
GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG 
CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT 
GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC 
CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG 
ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT 
TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC 
CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG 
TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT 
GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA 
GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA 
GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT 
CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA 
GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA 
GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC 
AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA 
CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC 
CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC 
CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA 
GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG 
ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA 
GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG 
GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT 
GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA 
AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC 
CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA 
GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG 
AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG 
GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG 
AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG 
GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC 
GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC 
TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT 
GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG 
GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG 
TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG 
GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC 
CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC 
TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT 
TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC 
ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT 
GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG 
CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC 
CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC 
ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG 
ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG 
GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG 
GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG 
ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC 
AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA 
GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC 
GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC 
AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT 
GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC 
ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT 
GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC 
ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT 
GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA 
GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC 
GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC 
GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG 
CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC 
CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC 
CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA 
GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG 
CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT 
AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG 
TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA 
AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC 
AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A3 ( XM_024990264.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846032.1 Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X3, mRNA.

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CloneID	OBa233422
Clone ID Related Accession (Same CDS sequence)	XM_024990264.1
Accession Version	XM_024990264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	collagen alpha-3(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGCA CAAGACTCTG CTGACATTAT TTTCCTTATT 
GATGGATCAA ACAACACCGG AAGTGTCAAT TTCGCAGTCA TTCTCGACTT CCTTGTAAAT 
CTCCTTGAGA GACTGTCAAT TGGAACTCAG CAGATCCGAG TGGGGGTGGT GCAGTATAGC 
GATGAGCCCA GAACCATGTT CTCCTTGAAC AGCTACTCCA CCAAGGCTCA GGTTCTGGAT 
GCAGTGAAGG CCCTGGGGTT CATTGGTGGA GAGCTGGCCA ATGTTGGCCT TGCCCTGGAT 
TTTGTGGTGG AGAATCACTT CACCCGGGCG GGGGGCAGCC GAGCAGAGGA AGGGGTTCCC 
CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATCCGAGA CGGGGTCGTA 
GCACTGAAGC AGGCTAGCGT TTTCTCGTTT GGCCTCGGAG CCCAGGCCGC CTCCAAGGCA 
GAGCTTCAGC ACATAGCTAC CAATGACAAC CTGGTGTTTA CTGTCCCGGA ATTCCGTAGC 
CTTGGGGACG TCCAGGAACA ATTACTGCCG TACATTGTTG GCGTGGCCCA AAGGCACATT 
GTCTTGCAAC CGCCAACCAT TGTCACCCAA GTCATTGAAG TCAACAAGAG GGACATAGTT 
TTCCTGGTGG ATGGCTCTTC CAAACTGGGA CTGACCAACT TCAATGCAAT CCGCGACTTC 
ATTGCCAAGG TCATCCAGAG GCTGGAGATC AGACAGGATC TTATCCAGGT GGCAGTGGCT 
CAGTATGCAG ACACAGTGAG GCCAGAGTTT TATTTCAATA CCTACCCCAG CAAGAGGGAG 
GTCATAAACG CCGTGCGGAA AATGAAGGCT TTGGATGGCT CAGCCTTGTA CACGGGCTCC 
GCTCTGGATT TTGTTCGGAA CAACCTGTTC ACGGAGGCGG CCGGCTACCG GGCTGCCGAG 
GGGGTCCCTA AGCTTCTGGT GCTGGTTACA GGCGGTAAGT CCCTAGATGC GGTCAGCCAG 
CCCGCCCAGG AGCTGAAGAG GAGCGGCATC CTGGCCTTCG CCGTCGGGAA CAAGGTGGCT 
GACCAGGCTG AACTGGAAGA GATCGCTTTC GATTCCTCCC TGGTGTTCAC TGCCACCGAG 
TTCCGACCCG CCCCTTTGCA GGGCGTGCTG CCCGGCTTGC TCGGGCCTCT CAGGACCCTC 
ACTGGAACCA CTGAAGTTCG CGTAAACAAA AGGGATATCA TCTTTCTTTT GGATGGATCG 
TCCAACGTCG GAGAGACCAA TTTCCCTTAT GTGCGGGACT TTGTCATGAA CCTAGTTAAC 
AGCCTTGATG TTGGAAGCGA CCACATTCGG GTCGGTCTAG TGCAATTTAG TGACACTCCG 
GTGACGGAGT TCTCCCTAAA CACCTACCCG ACCAAGTCAG AGTTGCTAGC TCACCTGAGG 
CAGATGCAGC TCCAGGGGGG GTCGGTCCTG AACACGGGCG CGGCCCTGAG CTATGTGCAT 
GCCAACCACT TCACCGAAGC TGGCGGCAGC AGGATCCAGG ACCACGTGCC GCAGCTGCTA 
CTCCTGCTCA CGGCCGGGCA GTCTGAGGAC TCCTATTTGC AAGCCGCCAA CGCCCTGGCG 
CGCGCGGGCA TCCTGACCTT TTGTGTGGGC ACTAGCCAGG CCGATAGGGC GGAGCTTGAG 
GAGATTGCTT TTAACCCGGG CCTGGTGTAT CTCATGGATG ATTTCAGCTC CTTGCCAGCT 
TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACTTATGTTA GCGGAGGTGT GGAGGAGGTG 
CCTCTTGCCC AGCCAGAGAG CAAGCGAGAC ATCCTGTTCC TTTTCGACGG CTCAGCCAAC 
CTCATGGGCC AGTTCACTGC CGCCCGTGAC TTTCTCTACA AGGTCATCGA CGAGCTGGAT 
GTGAAACCTG AGGGGACCCG GGTTGCAGTG GCTCAGTTCA GTGATGATGT CAAGGTGGAG 
TCCCGTTTTG ACCAGCACCA GAATAAGCCT GAGATCCTGA ATCTCGTGAA GAGGATGAAG 
CTCAAGACAG GCAAAGCCCT CAACCTGGGC TACGCCTTGG ATTACGCACA GAGGTACATC 
TTTGTGAAGT CCGCTGGAAG CCGGATTGAG GATGGAGTGC TTCAGTTCCT GGTGCTGCTG 
GTGGCGGGAA AGTCATCGGA CCGGGTGGAT ACACCCGCAC TCAACCTGAA GCAGAGCGGG 
GTGGTGCCCT TCATCCTCCA GGCGAAAAAC GCGGACCCTG CTGAGTTGGA GCTGATCGTA 
CCATCCCCAG CCTTTATTTT GGTTGCGGAG TCCCTTCCTA AGATTGGAGA CCTGCAACCA 
CAGATTGTGA ACCTCTTAAA ATCAGTGCAA AATGGGGCAC CGGCACCAGT TTCAGTTGAA 
AAGGATGTGG TGTTCCTGAT TGATGGCTCC GAGGGTGTCA GGAGCGGCTT CCCACTGCTG 
AAGGAGTTCG TCCAGAGAGT GGTGGAGAGC CTGGACGTGG GACCAGACCG GGTGCGTGTG 
GCCGTGGTGC AGTACAGCGA CCGGACCAGA CCTGAGTTCT ACCTGAATTC CTACATGGAC 
CAGCAGAGTG TGGTCGGAGC CATCCGTGGG CTGACCCTGC TGGGCGGGCC GGCCCCCAAC 
ACAGGGGCTG CCCTGGAGTT CGTCCTGAGG AACATCCTGG TGGGCTCTGC TGGGAGCCGC 
ATAGCAGAAG GTGTCCCCCA GCTCCTGATT GTCCTCACGG CCGACAGGTC TGGGGATGAC 
GTGAGGGGCC CCTCTGTGGT CCTGAGGAGG GGAGGGGCCG TGCCCATCGG CATCGGCATT 
GGGAACGCTG ACATCACCGA GATGCAGACC CTCTCCTTCG TCCCGGACTT CGCCGTGGTC 
ATCCCCACCT TCCGGCAGCT GGGGACCATC CAACAGGTCA TCTCGGAGAG AGTGACCCAG 
CTCAGCCGCG AGGAACTGAG CAGGCTGCAG GCGTCTGTCA CCCCCTTGAC GACTCCGGTG 
GTCAGCAGCA AGAGGGACGT GGTCTTTCTC ATCGATGGGT CCCAGAGTGC TGGCCCCGAG 
TTCCAGTACA TCCGAACGCT CATCGAGAGG CTGGTGGACT ACCTGGATGT GGGCTTCGAC 
ACGACCCGGG TGGCGGTCAT CCAGTTCAGC GATGACCCCA GGGTGGAGTT CCTGTTGAAC 
GTCCACTCCA GCAAGGACGA GGTGCAGAAC GCCGTGCGGC GGCTGCGGCC CAAAGGTGGG 
AGGCAGGTCA ACGTTGGGGG TGCCCTGGAG TACGTGGCCA GGAACATCTT CAAGAGGCCC 
CTGGGGAGCC GGATTGAGGA GGGTGTCCCT CAGTTCCTGG TCCTCATTTC CTCCGGGAAG 
TCGGATGATG AGGTGGAGGA CTCGGCCATA GAGCTCAAGC AGTTTGGCGT GGCGCCACTC 
ACGATCGCCA GGAACGTGGA CCAGGAAGAG CTGGTGAAGA TCTCGCTGAG CCCCGAGTAC 
GTGTTCTCAG TGAACACCTT CCGGGAGCTC CCCAGCCTGG AGCAGAAACT GCTGACACCG 
CTCACCACCC TGACTGCCGG GCAGATACAG CAGCTCCTGG CCAGCACGCG CTACCCGCCT 
CCAGCTGTGG AGAGCGATGC TGCGGACATT GTCTTCCTCA TCGACAGCTC TGATGGCGTG 
AAGCCGGATG GCATCGCACA CATCCGGGAC TTTGTTATCA GGATCGTGCG GAGACTCAAC 
GTCGGTCCCA ACAAAGTGAG AATTGGGGTC CTGCAGTTCA GCAACGACGT CTTCCCAGAA 
TTTCAACTAA AGACCTATAA ATCCCAGGCC AGCGTGCTGG ATGCCATACG TCGCCTGAGG 
TTCAAAGGGG GGTCTCCTCT CAACACTGGC AAGGCTCTGG AATTCGTGGC GAGGAATTAC 
TTTGTCAAGT CTGCCGGGAG CCGGATAGAA GATGGGGTGC CCCAACACCT GGTTCTATTT 
CTGGGTGGAA AATCCCAGGA TGATATTTCC AGGTATTCCC AGGTGATAAA ATCTGCAGGA 
ATTGCGAGCT TAGGGGTTGG AGACCGAAAT ATTGACAGAA CAGAGCTGCA GACCATCACC 
AGCGATCCCA GGCTTGTCTT CACGGTGCGG GAGTTCAGAG ACCTCCCCAG CATAGAAGAA 
AGGATGGTGA ATTCCTTTGG GTCCTCCGGG GTCACCCCTG CACCTCCGGG AGTGGACACA 
CCCTCTCCTT CAAGGCCAGA GAAGAAGAAA GCCGACATTG TGTTCCTGCT GGATGGTTCC 
ATCAATTTCC GGAGAGACAG TTTCCAGGAA GTGCTCCGTT TTGTGTCAGA AATTGTGGAC 
ACGGTTTATG AAGGGGGCGA CTCCATCCAG GTGGGGCTGG TCCAATACAA CTCTGACCCC 
ACCGACGAGT TCTTCCTGAA GGACTTCCCC ACCAAGCAGC AGATTATCGA CGCCATAAAC 
AAGGTGGTCT ACAAAGGGGG GAGGCACGCG AACACCAAGG TGGGCCTGGA GCACCTGCGG 
CGGAACCACT TCGTGCCCGA GGCGGGCAGC CGCCTGGACC AGAGGGTCCC GCAGATCGCC 
TTTGTGATCA CGGGGGGCAA GTCGGTGGAG GACGCTCAGG AGGCAAGCAT GGCGCTCACG 
CAGAGAGGCG TCAAGGTGTT TGCGGTGGGC GTAAGGAACA TCGACTCGGA GGAGGTTGGG 
AAGATAGCGT CCAACAGCGC CACGGCCTTC CGGGTGGGGA ACGTCCAGGA GCTGTCGGAA 
CTGAGCGAGC AGGTTTTGGA AACTCTGCAC GATGCAATGC ATGAGACCTT ATGTCCAGGC 
GTGACTGACG TTTCCAAAGC CTGTAACTTG GATGTGATTC TGGGCTTCGA TGGTTCAGGA 
GATCAGAATG TCTTTGTGGC TCAGAAGGGC CTCGAGCCCA AGGTGGACAC CATCCTGAGG 
AGAATCAGTC AGATGCAGAA GATCAGCTGC AGCGGCAGCC AGCTGCCCAC CGTGCGGGTG 
TCTGTGGTGG CCCTCACGCC CTCGGGCCCC GTTGAGGCCT TTGACTTTGC TGAGTACCAG 
CCGGAGCTGT TTGAGAAGTT CCAGAACATG CGTGCCCAGC ACCCCTACGT CCTCACTGCG 
GACACACTGA AGCTCTACCA GAACAAGTTC CGGCAGGCCT CGATGGACAA CGTGAAGGTG 
GTCATTCATT TTACCGATGG AGTGGATGGG GATCTGGCTG ATTTACAAAG AGCATCAGAG 
GAACTCCGAC AAGAAGGTGT CCGAGCTCTG ATCTTGGTGG GCCTCGAACG CGTCGCCAAC 
TTGGAGCAGC TCATGCAGCT GGAGTTCGGG CGAGGGTTCA CGTACAACCG GCCCCTGAGG 
CTGAACTTGC TGGACCTGGA CTACGAACTA GCCGAGCAGC TTGACAACAT TGCTGAGAAA 
GCCTGCTGTG GGATTCCCTG CAAATGCTCT GGACAAAGAG GAGACCGAGG GCCCATAGGC 
AGCATCGGGC CAAAGGGCAT TCCTGGAGAA GATGGCTACC GAGGTTATCC TGGTGACGAA 
GGTGGACCTG GTGAACGCGG TCCTCCTGGC GTGAACGGCA CTCAAGGTTT CCAGGGCTGC 
CCCGGGCAGA GAGGAGTCAA GGGCTCTCGT GGATTCCCGG GAGAGAAGGG TGAATTAGGA 
GAAATTGGAC TGGATGGTCT TGATGGTGAA GATGGCGACA AAGGGCTGCC CGGTACTTCT 
GGAGAGAAAG GGAGTCCAGG AAGGAGGGGC GACAAAGGAC CCAAAGGCGA CAAAGGGGAG 
AGAGGCGATG TCGGGATCAG AGGCGACCCG GGCAATTCAG GACAGGACAG CCAGCAGAGA 
GGAGCCAAAG GAGAAACCGG AGACATCGGC CCCGTGGGTC TCCCTGGGAG AGACGGGGTG 
TCTGGAGGGC CTGGAGAACC CGGGAAGAGC GGTGGCTTTG GCCGAAGGGG ACCAGCGGGA 
GCTAAGGGCA ACCAGGGCGG CCCCGGCCAG CAGGGCCCTG TAGGAGAGCA GGGGACCAGA 
GGCGCACAGG GTCCGCCTGG TCCCACCGGT CCTCCAGGCC TGATTGGTGA ACAGGGCATT 
CCCGGACCTC GGGGAGGCGG GGGAACCGCA GGTGCTCCTG GAGAACGCGG CAGAACCGGG 
CCCCTGGGAA GAAAGGGTGA GCCCGGAGAT CCAGGACCTA AGGGGAGCAT CGGGAACCGG 
GGTCCTCGTG GGGAGACGGG AGATGATGGG CGAGATGGAG TTGGCAGCGA AGGTCGCATA 
GGCAAGAAGG GAGAAAGAGG ATTCCCTGGA TACCCAGGTC CAAAGGGCAA TCCTGGTGAG 
CCAGGGACAG ACGGACCACC GGGACCCAAA GGCATCAGAG GCCGAAGGGG AAACTCGGGA 
CCTCCAGGGA TAGCTGGACA GAAGGGAGAC CCCGGTTACC CAGGGCCATC TGGTTACAAA 
GGCAGCAGAG GCGACTCAAT GGACCAATGT GCGCTGGTCC AGAGCATCAA AGATAAATGC 
CCTTGCTGTT ACGGGCCCCT GGAGTGCCCC GTCTTCCCGA CGGAGCTGGC TTTCGCCTTG 
GACACCTCAG AGGGGGTCAC CCAGGACAGG TTCAGCCAGA TGAGGGAGGT GGTCCTCAAG 
ATTCTGGACG ACCTGACCAT CGCCGAGAGC AACTGCCCGA GGGGGGCCCG CGTGGCCGTG 
GTCACTTACA ACAACGAGGT TACCACGGAG ATCCGCTTTG CCGACTCCAA GAAGAAGTCC 
GTCCTCCTGG ACAAGATCAA GAACCTGCAG GTGTCGCTGA CCTCCAAGCA GCAGAGCCTG 
GAAACCGCCA TGTCCTTTGT GGCCAGAAAC ACGTTTAAGC GTGTGCGGAG CGGGTTCCTG 
ATGAGGAAGG TGGCGGTGTT CTTCAGCAAC AAGCCCACGA GGGCGACCCC GCAGCTCCGG 
GAGGCTGTGC TCAAGCTCTC AGATGCTGGG ATCACACCCT TGTTCCTCAC AAGCCAGGAG 
GACCGGCAGC TGATCAATGC CCTACAGATC AATAACACAG CGGTGGGACA TGCCCTTGTC 
CTTCCTGCTG GCGGAGACCT CACAGACTTC TTGAAGAATG TCCTGACGTG TCACGTCTGC 
TTGGACATCT GCAACATTGA CCCATCCTGT GGATTCGGCA GCTGGAGGCC TTCCTTCAGG 
GACCGGAGGG CAGCAGGCAG TGATGTGGAC ATCGACATGG CTTTCATCTT AGATAGCTCC 
GAGTCCACCA CCCCGTTCCA GTTCAACGAG ATGAGGAAGT ACATCGAGTA CCTGGTCCGA 
CAGCTGGATG TGAGCCCAGA CCCCAAGGCC TCCCAGCACT TTGCCCGCGT GGCGGTGGTG 
CAGCATGCGC CCTACGAGGC GGTGGACAAC GCCAGTGTGC CGCCTGTGAA GGTGGAGTTC 
TCCCTGACCG ACTACGGCTC CAAAGAGAAG CTGCTGGCCT TCCTTGGCAG CAGGATGACC 
CAGCTGCAGG GGACCAGGGC TTTGGGCCGC GCCATCGACT ACACCATTGA GAACATCTTC 
GAAAGTGCTC CCAACCCCCG GGACCTGAAG ATCGTGGTGC TGATGCTGAC GGGCGAGGTG 
CAGAAGCAGC AGCTGGAGGA GGCCCAGAGA GCCATCCTAC AGGCCAAGTG CAAAGGCTAC 
TTCTTCGTGA TCCTGGGCAT TGGCAGGAAG GTGAATGTCA AGGAGCTGTA CAGCTCTGCC 
AGCGAGCCCA ACGACGTCTT CTTCAAGCTA GTGGACAAGT CGACCGAGCT CAACGAGGAG 
CCCCTAATGC GCTTCGGGAG GCTGCTGCCA TCCTTCGTCA GCAGTAAAGA TGCTTTCTAC 
CTGTCCCCAG ATATCAGGAA ACAGTGTGAT TGGTTCCAAG CAGACCAACC GGCAAAGAAT 
CTCGTGCAGT TTGGTTACAA ACAAATAAAT GTTCCTAATA ACGTTACTTC AAGTCCTACA 
TCTAAACTGG TGACCACAAC GAAGCCGGTG ATCACCACCA CCAAACCGAT CACAGTGGTG 
AACCTGCCAG CCTCAAAGCC AGCTGCCACG AGACCTGTGG ATGAGAGACC CGCAGCTGGG 
AGATCCGTGG CCACCAAGAT GGACAGTGCC AGGCCCGTGG CCACCAAGAC CGAGGCCACC 
AAACCCGTGG CCACCAAGAC CGAGGCCACC AAGACTGAAG CTGCCAAACC TGTGGTCACC 
AAGGCTGAGG CTGCAAAACC CACGGCCACC AAGACTGAAG CCACTAAGAC CGAGGCTGCA 
AAGCTCATGG CCACCAAGAC CGAGGCCACC AAACCTGTGG CTGCCAGGCC CGAGGCGGCA 
AAGACAGCCA CAGCCAGACC TGCAGCAGCC GCGAAGCCTG CAGCAGCCAA ACCTGCCCCT 
GCCAGACCCC CCACTGCTGC CAGATCCATG GCCACGAGGT CCGAGGCCCC CAGGCCCCAG 
GCAGTCAAAC TGGCTGCCAG CAGGCCCGGC GCTGCTAAGC CCGTGGTGAA GGCACCTCGC 
GAGATCCATG TGTCTGAGGT CACAGAGAAC AGCGCCAAGC TGCACTGGGA GAGGCCCGAG 
CCCCCCAGCC CTTACTTATA CAACCTCACC GTCACCTCGG CCCACGATCA GGCCGTGGTT 
CTGAAGCAGA ACCTCACTGT CACGGACCGC GTCATCGGGG GCCTGCTCCC CGGGCAGACT 
TACCACGTTA CGGTGACCTG CTCCCTGAGG TCTCAGGTCA GAGCCATCTA CCAAGGCAGC 
TTCAGCACGA AGAAAATTCA GCCTCCACCT CCACAAACAG AGCGATCAGC CTCTAGTGCA 
ACCATCAACC TAGTGGTGAG CGCAGACCGG TTGGCTGGGA GTAAAGCAGA TATATGTAAG 
TTGCCAAAGG ACGGCGGAAC TTGCAGAGAG TTTGTATTAA AGTGGTACTA TGATTCCGTA 
ACTGAAAACT GCGCCCGATT CTGGTATGGA GGCTGCGGTG GAAACGAAAA CAGATTTAAT 
TCGCAGGATG AATGTGAAAA GGTTTGCCCT CCTGTGCCCA TCAAGCAGCC CGGAGTTATC 
GCCGCGATGG GGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846032.1
CDS	277..9354
Translation	MRKHRHLPLVAILCLFFSGFSFTRGQQQPAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLERLSIGTQQIRVGVVQYSDEPRTMFSLNSYSTKAQVLDAVKALGFIGGELANVGLALDFVVENHFTRAGGSRAEEGVPQVLVLISAGPSSDEIRDGVVALKQASVFSFGLGAQAASKAELQHIATNDNLVFTVPEFRSLGDVQEQLLPYIVGVAQRHIVLQPPTIVTQVIEVNKRDIVFLVDGSSKLGLTNFNAIRDFIAKVIQRLEIRQDLIQVAVAQYADTVRPEFYFNTYPSKREVINAVRKMKALDGSALYTGSALDFVRNNLFTEAAGYRAAEGVPKLLVLVTGGKSLDAVSQPAQELKRSGILAFAVGNKVADQAELEEIAFDSSLVFTATEFRPAPLQGVLPGLLGPLRTLTGTTEVRVNKRDIIFLLDGSSNVGETNFPYVRDFVMNLVNSLDVGSDHIRVGLVQFSDTPVTEFSLNTYPTKSELLAHLRQMQLQGGSVLNTGAALSYVHANHFTEAGGSRIQDHVPQLLLLLTAGQSEDSYLQAANALARAGILTFCVGTSQADRAELEEIAFNPGLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLMGQFTAARDFLYKVIDELDVKPEGTRVAVAQFSDDVKVESRFDQHQNKPEILNLVKRMKLKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGKSSDRVDTPALNLKQSGVVPFILQAKNADPAELELIVPSPAFILVAESLPKIGDLQPQIVNLLKSVQNGAPAPVSVEKDVVFLIDGSEGVRSGFPLLKEFVQRVVESLDVGPDRVRVAVVQYSDRTRPEFYLNSYMDQQSVVGAIRGLTLLGGPAPNTGAALEFVLRNILVGSAGSRIAEGVPQLLIVLTADRSGDDVRGPSVVLRRGGAVPIGIGIGNADITEMQTLSFVPDFAVVIPTFRQLGTIQQVISERVTQLSREELSRLQASVTPLTTPVVSSKRDVVFLIDGSQSAGPEFQYIRTLIERLVDYLDVGFDTTRVAVIQFSDDPRVEFLLNVHSSKDEVQNAVRRLRPKGGRQVNVGGALEYVARNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVEDSAIELKQFGVAPLTIARNVDQEELVKISLSPEYVFSVNTFRELPSLEQKLLTPLTTLTAGQIQQLLASTRYPPPAVESDAADIVFLIDSSDGVKPDGIAHIRDFVIRIVRRLNVGPNKVRIGVLQFSNDVFPEFQLKTYKSQASVLDAIRRLRFKGGSPLNTGKALEFVARNYFVKSAGSRIEDGVPQHLVLFLGGKSQDDISRYSQVIKSAGIASLGVGDRNIDRTELQTITSDPRLVFTVREFRDLPSIEERMVNSFGSSGVTPAPPGVDTPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEGGDSIQVGLVQYNSDPTDEFFLKDFPTKQQIIDAINKVVYKGGRHANTKVGLEHLRRNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQEASMALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDVSKACNLDVILGFDGSGDQNVFVAQKGLEPKVDTILRRISQMQKISCSGSQLPTVRVSVVALTPSGPVEAFDFAEYQPELFEKFQNMRAQHPYVLTADTLKLYQNKFRQASMDNVKVVIHFTDGVDGDLADLQRASEELRQEGVRALILVGLERVANLEQLMQLEFGRGFTYNRPLRLNLLDLDYELAEQLDNIAEKACCGIPCKCSGQRGDRGPIGSIGPKGIPGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEDGDKGLPGTSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGNSGQDSQQRGAKGETGDIGPVGLPGRDGVSGGPGEPGKSGGFGRRGPAGAKGNQGGPGQQGPVGEQGTRGAQGPPGPTGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGDPGPKGSIGNRGPRGETGDDGRDGVGSEGRIGKKGERGFPGYPGPKGNPGEPGTDGPPGPKGIRGRRGNSGPPGIAGQKGDPGYPGPSGYKGSRGDSMDQCALVQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVTQDRFSQMREVVLKILDDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSVLLDKIKNLQVSLTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRATPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPAGGDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDMAFILDSSESTTPFQFNEMRKYIEYLVRQLDVSPDPKASQHFARVAVVQHAPYEAVDNASVPPVKVEFSLTDYGSKEKLLAFLGSRMTQLQGTRALGRAIDYTIENIFESAPNPRDLKIVVLMLTGEVQKQQLEEAQRAILQAKCKGYFFVILGIGRKVNVKELYSSASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFVSSKDAFYLSPDIRKQCDWFQADQPAKNLVQFGYKQINVPNNVTSSPTSKLVTTTKPVITTTKPITVVNLPASKPAATRPVDERPAAGRSVATKMDSARPVATKTEATKPVATKTEATKTEAAKPVVTKAEAAKPTATKTEATKTEAAKLMATKTEATKPVAARPEAAKTATARPAAAAKPAAAKPAPARPPTAARSMATRSEAPRPQAVKLAASRPGAAKPVVKAPREIHVSEVTENSAKLHWERPEPPSPYLYNLTVTSAHDQAVVLKQNLTVTDRVIGGLLPGQTYHVTVTCSLRSQVRAIYQGSFSTKKIQPPPPQTERSASSATINLVVSADRLAGSKADICKLPKDGGTCREFVLKWYYDSVTENCARFWYGGCGGNENRFNSQDECEKVCPPVPIKQPGVIAAMGT

Target ORF information:

RefSeq Version	XM_024990264.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990264.1

ORF Insert Sequence:

ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
TCCTTTACTC GTGGTCAACA ACAGCCAGCA CAAGACTCTG CTGACATTAT TTTCCTTATT 
GATGGATCAA ACAACACCGG AAGTGTCAAT TTCGCAGTCA TTCTCGACTT CCTTGTAAAT 
CTCCTTGAGA GACTGTCAAT TGGAACTCAG CAGATCCGAG TGGGGGTGGT GCAGTATAGC 
GATGAGCCCA GAACCATGTT CTCCTTGAAC AGCTACTCCA CCAAGGCTCA GGTTCTGGAT 
GCAGTGAAGG CCCTGGGGTT CATTGGTGGA GAGCTGGCCA ATGTTGGCCT TGCCCTGGAT 
TTTGTGGTGG AGAATCACTT CACCCGGGCG GGGGGCAGCC GAGCAGAGGA AGGGGTTCCC 
CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATCCGAGA CGGGGTCGTA 
GCACTGAAGC AGGCTAGCGT TTTCTCGTTT GGCCTCGGAG CCCAGGCCGC CTCCAAGGCA 
GAGCTTCAGC ACATAGCTAC CAATGACAAC CTGGTGTTTA CTGTCCCGGA ATTCCGTAGC 
CTTGGGGACG TCCAGGAACA ATTACTGCCG TACATTGTTG GCGTGGCCCA AAGGCACATT 
GTCTTGCAAC CGCCAACCAT TGTCACCCAA GTCATTGAAG TCAACAAGAG GGACATAGTT 
TTCCTGGTGG ATGGCTCTTC CAAACTGGGA CTGACCAACT TCAATGCAAT CCGCGACTTC 
ATTGCCAAGG TCATCCAGAG GCTGGAGATC AGACAGGATC TTATCCAGGT GGCAGTGGCT 
CAGTATGCAG ACACAGTGAG GCCAGAGTTT TATTTCAATA CCTACCCCAG CAAGAGGGAG 
GTCATAAACG CCGTGCGGAA AATGAAGGCT TTGGATGGCT CAGCCTTGTA CACGGGCTCC 
GCTCTGGATT TTGTTCGGAA CAACCTGTTC ACGGAGGCGG CCGGCTACCG GGCTGCCGAG 
GGGGTCCCTA AGCTTCTGGT GCTGGTTACA GGCGGTAAGT CCCTAGATGC GGTCAGCCAG 
CCCGCCCAGG AGCTGAAGAG GAGCGGCATC CTGGCCTTCG CCGTCGGGAA CAAGGTGGCT 
GACCAGGCTG AACTGGAAGA GATCGCTTTC GATTCCTCCC TGGTGTTCAC TGCCACCGAG 
TTCCGACCCG CCCCTTTGCA GGGCGTGCTG CCCGGCTTGC TCGGGCCTCT CAGGACCCTC 
ACTGGAACCA CTGAAGTTCG CGTAAACAAA AGGGATATCA TCTTTCTTTT GGATGGATCG 
TCCAACGTCG GAGAGACCAA TTTCCCTTAT GTGCGGGACT TTGTCATGAA CCTAGTTAAC 
AGCCTTGATG TTGGAAGCGA CCACATTCGG GTCGGTCTAG TGCAATTTAG TGACACTCCG 
GTGACGGAGT TCTCCCTAAA CACCTACCCG ACCAAGTCAG AGTTGCTAGC TCACCTGAGG 
CAGATGCAGC TCCAGGGGGG GTCGGTCCTG AACACGGGCG CGGCCCTGAG CTATGTGCAT 
GCCAACCACT TCACCGAAGC TGGCGGCAGC AGGATCCAGG ACCACGTGCC GCAGCTGCTA 
CTCCTGCTCA CGGCCGGGCA GTCTGAGGAC TCCTATTTGC AAGCCGCCAA CGCCCTGGCG 
CGCGCGGGCA TCCTGACCTT TTGTGTGGGC ACTAGCCAGG CCGATAGGGC GGAGCTTGAG 
GAGATTGCTT TTAACCCGGG CCTGGTGTAT CTCATGGATG ATTTCAGCTC CTTGCCAGCT 
TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACTTATGTTA GCGGAGGTGT GGAGGAGGTG 
CCTCTTGCCC AGCCAGAGAG CAAGCGAGAC ATCCTGTTCC TTTTCGACGG CTCAGCCAAC 
CTCATGGGCC AGTTCACTGC CGCCCGTGAC TTTCTCTACA AGGTCATCGA CGAGCTGGAT 
GTGAAACCTG AGGGGACCCG GGTTGCAGTG GCTCAGTTCA GTGATGATGT CAAGGTGGAG 
TCCCGTTTTG ACCAGCACCA GAATAAGCCT GAGATCCTGA ATCTCGTGAA GAGGATGAAG 
CTCAAGACAG GCAAAGCCCT CAACCTGGGC TACGCCTTGG ATTACGCACA GAGGTACATC 
TTTGTGAAGT CCGCTGGAAG CCGGATTGAG GATGGAGTGC TTCAGTTCCT GGTGCTGCTG 
GTGGCGGGAA AGTCATCGGA CCGGGTGGAT ACACCCGCAC TCAACCTGAA GCAGAGCGGG 
GTGGTGCCCT TCATCCTCCA GGCGAAAAAC GCGGACCCTG CTGAGTTGGA GCTGATCGTA 
CCATCCCCAG CCTTTATTTT GGTTGCGGAG TCCCTTCCTA AGATTGGAGA CCTGCAACCA 
CAGATTGTGA ACCTCTTAAA ATCAGTGCAA AATGGGGCAC CGGCACCAGT TTCAGTTGAA 
AAGGATGTGG TGTTCCTGAT TGATGGCTCC GAGGGTGTCA GGAGCGGCTT CCCACTGCTG 
AAGGAGTTCG TCCAGAGAGT GGTGGAGAGC CTGGACGTGG GACCAGACCG GGTGCGTGTG 
GCCGTGGTGC AGTACAGCGA CCGGACCAGA CCTGAGTTCT ACCTGAATTC CTACATGGAC 
CAGCAGAGTG TGGTCGGAGC CATCCGTGGG CTGACCCTGC TGGGCGGGCC GGCCCCCAAC 
ACAGGGGCTG CCCTGGAGTT CGTCCTGAGG AACATCCTGG TGGGCTCTGC TGGGAGCCGC 
ATAGCAGAAG GTGTCCCCCA GCTCCTGATT GTCCTCACGG CCGACAGGTC TGGGGATGAC 
GTGAGGGGCC CCTCTGTGGT CCTGAGGAGG GGAGGGGCCG TGCCCATCGG CATCGGCATT 
GGGAACGCTG ACATCACCGA GATGCAGACC CTCTCCTTCG TCCCGGACTT CGCCGTGGTC 
ATCCCCACCT TCCGGCAGCT GGGGACCATC CAACAGGTCA TCTCGGAGAG AGTGACCCAG 
CTCAGCCGCG AGGAACTGAG CAGGCTGCAG GCGTCTGTCA CCCCCTTGAC GACTCCGGTG 
GTCAGCAGCA AGAGGGACGT GGTCTTTCTC ATCGATGGGT CCCAGAGTGC TGGCCCCGAG 
TTCCAGTACA TCCGAACGCT CATCGAGAGG CTGGTGGACT ACCTGGATGT GGGCTTCGAC 
ACGACCCGGG TGGCGGTCAT CCAGTTCAGC GATGACCCCA GGGTGGAGTT CCTGTTGAAC 
GTCCACTCCA GCAAGGACGA GGTGCAGAAC GCCGTGCGGC GGCTGCGGCC CAAAGGTGGG 
AGGCAGGTCA ACGTTGGGGG TGCCCTGGAG TACGTGGCCA GGAACATCTT CAAGAGGCCC 
CTGGGGAGCC GGATTGAGGA GGGTGTCCCT CAGTTCCTGG TCCTCATTTC CTCCGGGAAG 
TCGGATGATG AGGTGGAGGA CTCGGCCATA GAGCTCAAGC AGTTTGGCGT GGCGCCACTC 
ACGATCGCCA GGAACGTGGA CCAGGAAGAG CTGGTGAAGA TCTCGCTGAG CCCCGAGTAC 
GTGTTCTCAG TGAACACCTT CCGGGAGCTC CCCAGCCTGG AGCAGAAACT GCTGACACCG 
CTCACCACCC TGACTGCCGG GCAGATACAG CAGCTCCTGG CCAGCACGCG CTACCCGCCT 
CCAGCTGTGG AGAGCGATGC TGCGGACATT GTCTTCCTCA TCGACAGCTC TGATGGCGTG 
AAGCCGGATG GCATCGCACA CATCCGGGAC TTTGTTATCA GGATCGTGCG GAGACTCAAC 
GTCGGTCCCA ACAAAGTGAG AATTGGGGTC CTGCAGTTCA GCAACGACGT CTTCCCAGAA 
TTTCAACTAA AGACCTATAA ATCCCAGGCC AGCGTGCTGG ATGCCATACG TCGCCTGAGG 
TTCAAAGGGG GGTCTCCTCT CAACACTGGC AAGGCTCTGG AATTCGTGGC GAGGAATTAC 
TTTGTCAAGT CTGCCGGGAG CCGGATAGAA GATGGGGTGC CCCAACACCT GGTTCTATTT 
CTGGGTGGAA AATCCCAGGA TGATATTTCC AGGTATTCCC AGGTGATAAA ATCTGCAGGA 
ATTGCGAGCT TAGGGGTTGG AGACCGAAAT ATTGACAGAA CAGAGCTGCA GACCATCACC 
AGCGATCCCA GGCTTGTCTT CACGGTGCGG GAGTTCAGAG ACCTCCCCAG CATAGAAGAA 
AGGATGGTGA ATTCCTTTGG GTCCTCCGGG GTCACCCCTG CACCTCCGGG AGTGGACACA 
CCCTCTCCTT CAAGGCCAGA GAAGAAGAAA GCCGACATTG TGTTCCTGCT GGATGGTTCC 
ATCAATTTCC GGAGAGACAG TTTCCAGGAA GTGCTCCGTT TTGTGTCAGA AATTGTGGAC 
ACGGTTTATG AAGGGGGCGA CTCCATCCAG GTGGGGCTGG TCCAATACAA CTCTGACCCC 
ACCGACGAGT TCTTCCTGAA GGACTTCCCC ACCAAGCAGC AGATTATCGA CGCCATAAAC 
AAGGTGGTCT ACAAAGGGGG GAGGCACGCG AACACCAAGG TGGGCCTGGA GCACCTGCGG 
CGGAACCACT TCGTGCCCGA GGCGGGCAGC CGCCTGGACC AGAGGGTCCC GCAGATCGCC 
TTTGTGATCA CGGGGGGCAA GTCGGTGGAG GACGCTCAGG AGGCAAGCAT GGCGCTCACG 
CAGAGAGGCG TCAAGGTGTT TGCGGTGGGC GTAAGGAACA TCGACTCGGA GGAGGTTGGG 
AAGATAGCGT CCAACAGCGC CACGGCCTTC CGGGTGGGGA ACGTCCAGGA GCTGTCGGAA 
CTGAGCGAGC AGGTTTTGGA AACTCTGCAC GATGCAATGC ATGAGACCTT ATGTCCAGGC 
GTGACTGACG TTTCCAAAGC CTGTAACTTG GATGTGATTC TGGGCTTCGA TGGTTCAGGA 
GATCAGAATG TCTTTGTGGC TCAGAAGGGC CTCGAGCCCA AGGTGGACAC CATCCTGAGG 
AGAATCAGTC AGATGCAGAA GATCAGCTGC AGCGGCAGCC AGCTGCCCAC CGTGCGGGTG 
TCTGTGGTGG CCCTCACGCC CTCGGGCCCC GTTGAGGCCT TTGACTTTGC TGAGTACCAG 
CCGGAGCTGT TTGAGAAGTT CCAGAACATG CGTGCCCAGC ACCCCTACGT CCTCACTGCG 
GACACACTGA AGCTCTACCA GAACAAGTTC CGGCAGGCCT CGATGGACAA CGTGAAGGTG 
GTCATTCATT TTACCGATGG AGTGGATGGG GATCTGGCTG ATTTACAAAG AGCATCAGAG 
GAACTCCGAC AAGAAGGTGT CCGAGCTCTG ATCTTGGTGG GCCTCGAACG CGTCGCCAAC 
TTGGAGCAGC TCATGCAGCT GGAGTTCGGG CGAGGGTTCA CGTACAACCG GCCCCTGAGG 
CTGAACTTGC TGGACCTGGA CTACGAACTA GCCGAGCAGC TTGACAACAT TGCTGAGAAA 
GCCTGCTGTG GGATTCCCTG CAAATGCTCT GGACAAAGAG GAGACCGAGG GCCCATAGGC 
AGCATCGGGC CAAAGGGCAT TCCTGGAGAA GATGGCTACC GAGGTTATCC TGGTGACGAA 
GGTGGACCTG GTGAACGCGG TCCTCCTGGC GTGAACGGCA CTCAAGGTTT CCAGGGCTGC 
CCCGGGCAGA GAGGAGTCAA GGGCTCTCGT GGATTCCCGG GAGAGAAGGG TGAATTAGGA 
GAAATTGGAC TGGATGGTCT TGATGGTGAA GATGGCGACA AAGGGCTGCC CGGTACTTCT 
GGAGAGAAAG GGAGTCCAGG AAGGAGGGGC GACAAAGGAC CCAAAGGCGA CAAAGGGGAG 
AGAGGCGATG TCGGGATCAG AGGCGACCCG GGCAATTCAG GACAGGACAG CCAGCAGAGA 
GGAGCCAAAG GAGAAACCGG AGACATCGGC CCCGTGGGTC TCCCTGGGAG AGACGGGGTG 
TCTGGAGGGC CTGGAGAACC CGGGAAGAGC GGTGGCTTTG GCCGAAGGGG ACCAGCGGGA 
GCTAAGGGCA ACCAGGGCGG CCCCGGCCAG CAGGGCCCTG TAGGAGAGCA GGGGACCAGA 
GGCGCACAGG GTCCGCCTGG TCCCACCGGT CCTCCAGGCC TGATTGGTGA ACAGGGCATT 
CCCGGACCTC GGGGAGGCGG GGGAACCGCA GGTGCTCCTG GAGAACGCGG CAGAACCGGG 
CCCCTGGGAA GAAAGGGTGA GCCCGGAGAT CCAGGACCTA AGGGGAGCAT CGGGAACCGG 
GGTCCTCGTG GGGAGACGGG AGATGATGGG CGAGATGGAG TTGGCAGCGA AGGTCGCATA 
GGCAAGAAGG GAGAAAGAGG ATTCCCTGGA TACCCAGGTC CAAAGGGCAA TCCTGGTGAG 
CCAGGGACAG ACGGACCACC GGGACCCAAA GGCATCAGAG GCCGAAGGGG AAACTCGGGA 
CCTCCAGGGA TAGCTGGACA GAAGGGAGAC CCCGGTTACC CAGGGCCATC TGGTTACAAA 
GGCAGCAGAG GCGACTCAAT GGACCAATGT GCGCTGGTCC AGAGCATCAA AGATAAATGC 
CCTTGCTGTT ACGGGCCCCT GGAGTGCCCC GTCTTCCCGA CGGAGCTGGC TTTCGCCTTG 
GACACCTCAG AGGGGGTCAC CCAGGACAGG TTCAGCCAGA TGAGGGAGGT GGTCCTCAAG 
ATTCTGGACG ACCTGACCAT CGCCGAGAGC AACTGCCCGA GGGGGGCCCG CGTGGCCGTG 
GTCACTTACA ACAACGAGGT TACCACGGAG ATCCGCTTTG CCGACTCCAA GAAGAAGTCC 
GTCCTCCTGG ACAAGATCAA GAACCTGCAG GTGTCGCTGA CCTCCAAGCA GCAGAGCCTG 
GAAACCGCCA TGTCCTTTGT GGCCAGAAAC ACGTTTAAGC GTGTGCGGAG CGGGTTCCTG 
ATGAGGAAGG TGGCGGTGTT CTTCAGCAAC AAGCCCACGA GGGCGACCCC GCAGCTCCGG 
GAGGCTGTGC TCAAGCTCTC AGATGCTGGG ATCACACCCT TGTTCCTCAC AAGCCAGGAG 
GACCGGCAGC TGATCAATGC CCTACAGATC AATAACACAG CGGTGGGACA TGCCCTTGTC 
CTTCCTGCTG GCGGAGACCT CACAGACTTC TTGAAGAATG TCCTGACGTG TCACGTCTGC 
TTGGACATCT GCAACATTGA CCCATCCTGT GGATTCGGCA GCTGGAGGCC TTCCTTCAGG 
GACCGGAGGG CAGCAGGCAG TGATGTGGAC ATCGACATGG CTTTCATCTT AGATAGCTCC 
GAGTCCACCA CCCCGTTCCA GTTCAACGAG ATGAGGAAGT ACATCGAGTA CCTGGTCCGA 
CAGCTGGATG TGAGCCCAGA CCCCAAGGCC TCCCAGCACT TTGCCCGCGT GGCGGTGGTG 
CAGCATGCGC CCTACGAGGC GGTGGACAAC GCCAGTGTGC CGCCTGTGAA GGTGGAGTTC 
TCCCTGACCG ACTACGGCTC CAAAGAGAAG CTGCTGGCCT TCCTTGGCAG CAGGATGACC 
CAGCTGCAGG GGACCAGGGC TTTGGGCCGC GCCATCGACT ACACCATTGA GAACATCTTC 
GAAAGTGCTC CCAACCCCCG GGACCTGAAG ATCGTGGTGC TGATGCTGAC GGGCGAGGTG 
CAGAAGCAGC AGCTGGAGGA GGCCCAGAGA GCCATCCTAC AGGCCAAGTG CAAAGGCTAC 
TTCTTCGTGA TCCTGGGCAT TGGCAGGAAG GTGAATGTCA AGGAGCTGTA CAGCTCTGCC 
AGCGAGCCCA ACGACGTCTT CTTCAAGCTA GTGGACAAGT CGACCGAGCT CAACGAGGAG 
CCCCTAATGC GCTTCGGGAG GCTGCTGCCA TCCTTCGTCA GCAGTAAAGA TGCTTTCTAC 
CTGTCCCCAG ATATCAGGAA ACAGTGTGAT TGGTTCCAAG CAGACCAACC GGCAAAGAAT 
CTCGTGCAGT TTGGTTACAA ACAAATAAAT GTTCCTAATA ACGTTACTTC AAGTCCTACA 
TCTAAACTGG TGACCACAAC GAAGCCGGTG ATCACCACCA CCAAACCGAT CACAGTGGTG 
AACCTGCCAG CCTCAAAGCC AGCTGCCACG AGACCTGTGG ATGAGAGACC CGCAGCTGGG 
AGATCCGTGG CCACCAAGAT GGACAGTGCC AGGCCCGTGG CCACCAAGAC CGAGGCCACC 
AAACCCGTGG CCACCAAGAC CGAGGCCACC AAGACTGAAG CTGCCAAACC TGTGGTCACC 
AAGGCTGAGG CTGCAAAACC CACGGCCACC AAGACTGAAG CCACTAAGAC CGAGGCTGCA 
AAGCTCATGG CCACCAAGAC CGAGGCCACC AAACCTGTGG CTGCCAGGCC CGAGGCGGCA 
AAGACAGCCA CAGCCAGACC TGCAGCAGCC GCGAAGCCTG CAGCAGCCAA ACCTGCCCCT 
GCCAGACCCC CCACTGCTGC CAGATCCATG GCCACGAGGT CCGAGGCCCC CAGGCCCCAG 
GCAGTCAAAC TGGCTGCCAG CAGGCCCGGC GCTGCTAAGC CCGTGGTGAA GGCACCTCGC 
GAGATCCATG TGTCTGAGGT CACAGAGAAC AGCGCCAAGC TGCACTGGGA GAGGCCCGAG 
CCCCCCAGCC CTTACTTATA CAACCTCACC GTCACCTCGG CCCACGATCA GGCCGTGGTT 
CTGAAGCAGA ACCTCACTGT CACGGACCGC GTCATCGGGG GCCTGCTCCC CGGGCAGACT 
TACCACGTTA CGGTGACCTG CTCCCTGAGG TCTCAGGTCA GAGCCATCTA CCAAGGCAGC 
TTCAGCACGA AGAAAATTCA GCCTCCACCT CCACAAACAG AGCGATCAGC CTCTAGTGCA 
ACCATCAACC TAGTGGTGAG CGCAGACCGG TTGGCTGGGA GTAAAGCAGA TATATGTAAG 
TTGCCAAAGG ACGGCGGAAC TTGCAGAGAG TTTGTATTAA AGTGGTACTA TGATTCCGTA 
ACTGAAAACT GCGCCCGATT CTGGTATGGA GGCTGCGGTG GAAACGAAAA CAGATTTAAT 
TCGCAGGATG AATGTGAAAA GGTTTGCCCT CCTGTGCCCA TCAAGCAGCC CGGAGTTATC 
GCCGCGATGG GGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	COL6A3
Entrez Gene ID	530657
Full Name	collagen type VI alpha 3 chain
General protein information	Preferred Names collagen type VI alpha 3 chain Names collagen alpha-3(VI) chain alpha 3 type VI collagen collagen, type VI, alpha 3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	COL6A3	XP_005205035.1
Canis lupus familiaris (dog)	COL6A3	NP_001096685.1
Mus musculus (house mouse)	Col6a3	NP_001229937.1
Xenopus tropicalis (tropical clawed frog)	col6a3	XP_002932067.2
Homo sapiens (human)	COL6A3	NP_004360.2
Pan troglodytes (chimpanzee)	COL6A3	XP_001153544.1
Macaca mulatta (Rhesus monkey)	COL6A3	XP_001084624.1
Rattus norvegicus (Norway rat)	Col6a3	XP_003750780.1
Gallus gallus (chicken)	COL6A3	NP_990865.1
Danio rerio (zebrafish)	col6a3	XP_002663415.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

COL6A3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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