ARHGAP28 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGAP28
Entrez Gene ID	530550
Full Name	Rho GTPase activating protein 28
General protein information	Preferred Names Rho GTPase activating protein 28 Names rho GTPase-activating protein 28
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010818945.3	XP_010817247.1	rho GTPase-activating protein 28 isoform X1
XM_010825551.2	XP_010823853.2	rho GTPase-activating protein 28 isoform X2
XM_010818945.2	XP_010817247.1	rho GTPase-activating protein 28 isoform X1
XM_010818947.2	XP_010817249.1	rho GTPase-activating protein 28 isoform X2
XM_010825549.2	XP_010823851.2	rho GTPase-activating protein 28 isoform X1
XM_010818947.3	XP_010817249.1	rho GTPase-activating protein 28 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARHGAP28	NP_001010000.1
Canis lupus familiaris (dog)	ARHGAP28	XP_005623121.1
Mus musculus (house mouse)	Arhgap28	NP_766552.3
Gallus gallus (chicken)	ARHGAP28	XP_419140.3
Xenopus tropicalis (tropical clawed frog)	arhgap28	XP_002938100.2
Pan troglodytes (chimpanzee)	ARHGAP28	XP_003315857.1
Macaca mulatta (Rhesus monkey)	ARHGAP28	XP_002800906.1
Bos taurus (cattle)	ARHGAP28	XP_002697842.2
Rattus norvegicus (Norway rat)	Arhgap28	NP_001178744.1
Danio rerio (zebrafish)	arhgap28	NP_001007436.1

Gene Ontology

The following ARHGAP28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGAP28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa230008	XM_010818945.3 Latest version!	Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa230009	XM_010818947.3 Latest version!	Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa51832	XM_010825549.2 Latest version!	Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa51832	XM_010818945.2 Latest version!	Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa51833	XM_010818947.2 Latest version!	Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa51833	XM_010825551.2 Latest version!	Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGAP28 ( XM_010818945.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817247.1 Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa230008
Clone ID Related Accession (Same CDS sequence)	XM_010818945.3
Accession Version	XM_010818945.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818945.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTCC CCGCCTTTGT TCTCCGGCCG GAGCGGAGCC ATGCGCGGCT GACGATGGAA 
GTGGAGGACT CGGGCGGCGT GGTGCTCACC GCCTACCACT CGTACGCGCG CCCCCCGCCG 
CCCAGCCCGG AGCCGCGCTG CGCGCCCCGG GCGACCGCCA GCCTCCCCGG CAGCAGAAAG 
TCCATCCCTC GCTGCCGAAG AATCAACAGG ATGCTCTCCA ATGAGTCCCT TCAGTCCCCG 
GCGTTCAGCC GCTCCAACTC TCAAGCCTCC GTAGACAGCG CCTCCATGGA GAATTTCTGG 
CGGGAAATAG AAAGCATCAA AGAGACCAGC CTCGGGGCTC AGGAGGAACA GACGCCTGCC 
GAGGCCAAGC CCCTGGACGA AGGAGAACTT GAAGCAGAGT GGTTGCAAGA TGTTGGTTTA 
TCAACCCTGA TCTCGGGTGA TGAGGAGGAA GATGGCAAAG CCTTGCTCTC TACTTTGACT 
CGAACCCAGG CAGCTGCAGT GAAGAAGAGA TACAATACTT ACACACAAAC CTTGAGGAAA 
AAGAACAAGC AGTCTGTCAG GGATGTCAGA GACATCTTCG GAACCAGCGA ATCTCCTCCA 
GATGAGTTTT GCTGTGTCCC AGCTCCTCTT CTGGATGTGA GCCCGGATGA AGAAAGGATG 
CCAACGGTCC CTTGCAGAAA TGGTCAGCTG CCAGGTGATA CCTGTGACAG CCACGCTAGC 
GAGCTGGATG GCGCCCGGGA GGGGAAAGCG CTGCCAGGAA TCATCAAAGC CAGCGGCCCC 
TTGCCAGATG GTGCTTCTCT CAACAGTAGT ACCCTGTCAG ATGGGTCCCA GGATGAAGAA 
TGGAGTTTTA CTGTTCCCAG GAGTGGCTCC ATGTCCATCC TGGAGGCCAT TCCAGACGTG 
CCAGTTCATT CCAACGTCGC GGCAGACCCT GGACGGTCGG CGCGGAACGC TGCAAGTGAT 
GATGAATATC TGGAAAAAAA CATTCCGGTA GAAGCTGAAG AGCTGTCCTT TGAAGTATCA 
TATTCAGAAA AGCTAACACA GGCTCCAAAA AGGAACAGAT TTAAGAAGTT AGATTTTAAG 
AAAGAAGACT ATGTTTTACC TAAATTTGTT GTTCAGAAAA CCAGATTTGG CCTAACCAAA 
GCAGAAGATC TGTCTGCGGA AGACATGAAG AAAATCCGCC ATCTCTCTCT GATTGAATTG 
ACGGCCTTTT TTGATGCCTT TCGAATTCAG CTGAAAAGAA ACAAAACAGA GAAAGTCAAA 
GGACGAGACA GTGGGATTTT TGGAGTTCCC CTCACAGTCC TGCTAGAAAA TGACCGAAGG 
AAAGACTCTG GGGTCAAAGT TCCCCTTGTG TTACAAAAGT TCTTTGAGAA AGTTGAGGAA 
TCGGGTCTGG AATCTGAAGG GATCTTTCGA CTTTCAGGAT GTACTGCCAA AGTCAAGCAA 
TACCGTGAAG AACTGGATGC CAAGTTTAAT GCTGACAAGT TTAAATGGGA GAAAATGTGC 
CATAGAGAAG CTGCAGTGAT GCTGAAAGCC TTTTTCAGAG AACTGCCCAC CTCTCTCTTT 
CCCGTGGAAT ATATACCTGC CTTCATCACT CTAATGGAAA GAGGGCCTCA CATCCGAGTA 
CAGTTTCAAG CCTTACACCT CATGGTCATG GCACTGCCTG ATGCCAACAG GGACACAGCC 
CAGGCCCTCA TGACATTCTT CAATAAAGTC ATTGCCAATG AATCAAAAAA TCGCATGAGT 
ATATGGAACA TTTCTACTGT CATGGCACCA AATCTTTTCT TCAGTAGAAG CAAACATTCT 
GACTGTGAAG AATTACTCTT AGCAAACACT GCTGCCCACA TCATCCGTCT GATGCTCAAG 
TACCAGGAGA TGTTGTGGAC GGTTCCGTCT TTCTTAATCT CTCAAGTCAG AAGAATGAAT 
GAAGCCACCA TGTTGTTAAA GAAGCCGCTC CCAAGTGTCA AAAAGCTGCT AAGGAGGAAG 
ACCATAGAAC GAGGGGTTAC AAGCCCCAAG ACATCAAAGG TACTGCAAAA ATCACCCTCT 
ACAAGAAGAA TGTCCGACGT GCCAGAGGGC GTCATAAGGG TCCACGCGCC CCTTCTCTCC 
AAGGTGTCCA TGGCCATTCA ACTCAACAGT CAAACCAAAG TCAAAGACAT CCTGGCAAAA 
TTTCAATGTG AGAACAGTCA TGGTTCATCG GAATGCATTA AGATCCAGAA CCAAAGGTTA 
TATGAAATTG GAGGTAATAT AGGACAGCAC TGCTTGGATC CGGATGCTTA TATACTGGAT 
GTTTATCATG TGAATCCCCA GGCAGAATGG GTGATTAAAC CCCAACAGAG TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817247.1
CDS	436..2772
Translation	MLVPAFVLRPERSHARLTMEVEDSGGVVLTAYHSYARPPPPSPEPRCAPRATASLPGSRKSIPRCRRINRMLSNESLQSPAFSRSNSQASVDSASMENFWREIESIKETSLGAQEEQTPAEAKPLDEGELEAEWLQDVGLSTLISGDEEEDGKALLSTLTRTQAAAVKKRYNTYTQTLRKKNKQSVRDVRDIFGTSESPPDEFCCVPAPLLDVSPDEERMPTVPCRNGQLPGDTCDSHASELDGAREGKALPGIIKASGPLPDGASLNSSTLSDGSQDEEWSFTVPRSGSMSILEAIPDVPVHSNVAADPGRSARNAASDDEYLEKNIPVEAEELSFEVSYSEKLTQAPKRNRFKKLDFKKEDYVLPKFVVQKTRFGLTKAEDLSAEDMKKIRHLSLIELTAFFDAFRIQLKRNKTEKVKGRDSGIFGVPLTVLLENDRRKDSGVKVPLVLQKFFEKVEESGLESEGIFRLSGCTAKVKQYREELDAKFNADKFKWEKMCHREAAVMLKAFFRELPTSLFPVEYIPAFITLMERGPHIRVQFQALHLMVMALPDANRDTAQALMTFFNKVIANESKNRMSIWNISTVMAPNLFFSRSKHSDCEELLLANTAAHIIRLMLKYQEMLWTVPSFLISQVRRMNEATMLLKKPLPSVKKLLRRKTIERGVTSPKTSKVLQKSPSTRRMSDVPEGVIRVHAPLLSKVSMAIQLNSQTKVKDILAKFQCENSHGSSECIKIQNQRLYEIGGNIGQHCLDPDAYILDVYHVNPQAEWVIKPQQSV

Target ORF information:

RefSeq Version	XM_010818945.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818945.3

ORF Insert Sequence:

ATGTTGGTCC CCGCCTTTGT TCTCCGGCCG GAGCGGAGCC ATGCGCGGCT GACGATGGAA 
GTGGAGGACT CGGGCGGCGT GGTGCTCACC GCCTACCACT CGTACGCGCG CCCCCCGCCG 
CCCAGCCCGG AGCCGCGCTG CGCGCCCCGG GCGACCGCCA GCCTCCCCGG CAGCAGAAAG 
TCCATCCCTC GCTGCCGAAG AATCAACAGG ATGCTCTCCA ATGAGTCCCT TCAGTCCCCG 
GCGTTCAGCC GCTCCAACTC TCAAGCCTCC GTAGACAGCG CCTCCATGGA GAATTTCTGG 
CGGGAAATAG AAAGCATCAA AGAGACCAGC CTCGGGGCTC AGGAGGAACA GACGCCTGCC 
GAGGCCAAGC CCCTGGACGA AGGAGAACTT GAAGCAGAGT GGTTGCAAGA TGTTGGTTTA 
TCAACCCTGA TCTCGGGTGA TGAGGAGGAA GATGGCAAAG CCTTGCTCTC TACTTTGACT 
CGAACCCAGG CAGCTGCAGT GAAGAAGAGA TACAATACTT ACACACAAAC CTTGAGGAAA 
AAGAACAAGC AGTCTGTCAG GGATGTCAGA GACATCTTCG GAACCAGCGA ATCTCCTCCA 
GATGAGTTTT GCTGTGTCCC AGCTCCTCTT CTGGATGTGA GCCCGGATGA AGAAAGGATG 
CCAACGGTCC CTTGCAGAAA TGGTCAGCTG CCAGGTGATA CCTGTGACAG CCACGCTAGC 
GAGCTGGATG GCGCCCGGGA GGGGAAAGCG CTGCCAGGAA TCATCAAAGC CAGCGGCCCC 
TTGCCAGATG GTGCTTCTCT CAACAGTAGT ACCCTGTCAG ATGGGTCCCA GGATGAAGAA 
TGGAGTTTTA CTGTTCCCAG GAGTGGCTCC ATGTCCATCC TGGAGGCCAT TCCAGACGTG 
CCAGTTCATT CCAACGTCGC GGCAGACCCT GGACGGTCGG CGCGGAACGC TGCAAGTGAT 
GATGAATATC TGGAAAAAAA CATTCCGGTA GAAGCTGAAG AGCTGTCCTT TGAAGTATCA 
TATTCAGAAA AGCTAACACA GGCTCCAAAA AGGAACAGAT TTAAGAAGTT AGATTTTAAG 
AAAGAAGACT ATGTTTTACC TAAATTTGTT GTTCAGAAAA CCAGATTTGG CCTAACCAAA 
GCAGAAGATC TGTCTGCGGA AGACATGAAG AAAATCCGCC ATCTCTCTCT GATTGAATTG 
ACGGCCTTTT TTGATGCCTT TCGAATTCAG CTGAAAAGAA ACAAAACAGA GAAAGTCAAA 
GGACGAGACA GTGGGATTTT TGGAGTTCCC CTCACAGTCC TGCTAGAAAA TGACCGAAGG 
AAAGACTCTG GGGTCAAAGT TCCCCTTGTG TTACAAAAGT TCTTTGAGAA AGTTGAGGAA 
TCGGGTCTGG AATCTGAAGG GATCTTTCGA CTTTCAGGAT GTACTGCCAA AGTCAAGCAA 
TACCGTGAAG AACTGGATGC CAAGTTTAAT GCTGACAAGT TTAAATGGGA GAAAATGTGC 
CATAGAGAAG CTGCAGTGAT GCTGAAAGCC TTTTTCAGAG AACTGCCCAC CTCTCTCTTT 
CCCGTGGAAT ATATACCTGC CTTCATCACT CTAATGGAAA GAGGGCCTCA CATCCGAGTA 
CAGTTTCAAG CCTTACACCT CATGGTCATG GCACTGCCTG ATGCCAACAG GGACACAGCC 
CAGGCCCTCA TGACATTCTT CAATAAAGTC ATTGCCAATG AATCAAAAAA TCGCATGAGT 
ATATGGAACA TTTCTACTGT CATGGCACCA AATCTTTTCT TCAGTAGAAG CAAACATTCT 
GACTGTGAAG AATTACTCTT AGCAAACACT GCTGCCCACA TCATCCGTCT GATGCTCAAG 
TACCAGGAGA TGTTGTGGAC GGTTCCGTCT TTCTTAATCT CTCAAGTCAG AAGAATGAAT 
GAAGCCACCA TGTTGTTAAA GAAGCCGCTC CCAAGTGTCA AAAAGCTGCT AAGGAGGAAG 
ACCATAGAAC GAGGGGTTAC AAGCCCCAAG ACATCAAAGG TACTGCAAAA ATCACCCTCT 
ACAAGAAGAA TGTCCGACGT GCCAGAGGGC GTCATAAGGG TCCACGCGCC CCTTCTCTCC 
AAGGTGTCCA TGGCCATTCA ACTCAACAGT CAAACCAAAG TCAAAGACAT CCTGGCAAAA 
TTTCAATGTG AGAACAGTCA TGGTTCATCG GAATGCATTA AGATCCAGAA CCAAAGGTTA 
TATGAAATTG GAGGTAATAT AGGACAGCAC TGCTTGGATC CGGATGCTTA TATACTGGAT 
GTTTATCATG TGAATCCCCA GGCAGAATGG GTGATTAAAC CCCAACAGAG TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP28 ( XM_010818947.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817249.1 Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa230009
Clone ID Related Accession (Same CDS sequence)	XM_010818947.3
Accession Version	XM_010818947.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818947.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCA ATGAGTCCCT TCAGTCCCCG GCGTTCAGCC GCTCCAACTC TCAAGCCTCC 
GTAGACAGCG CCTCCATGGA GAATTTCTGG CGGGAAATAG AAAGCATCAA AGAGACCAGC 
CTCGGGGCTC AGGAGGAACA GACGCCTGCC GAGGCCAAGC CCCTGGACGA AGGAGAACTT 
GAAGCAGAGT GGTTGCAAGA TGTTGGTTTA TCAACCCTGA TCTCGGGTGA TGAGGAGGAA 
GATGGCAAAG CCTTGCTCTC TACTTTGACT CGAACCCAGG CAGCTGCAGT GAAGAAGAGA 
TACAATACTT ACACACAAAC CTTGAGGAAA AAGAACAAGC AGTCTGTCAG GGATGTCAGA 
GACATCTTCG GAACCAGCGA ATCTCCTCCA GATGAGTTTT GCTGTGTCCC AGCTCCTCTT 
CTGGATGTGA GCCCGGATGA AGAAAGGATG CCAACGGTCC CTTGCAGAAA TGGTCAGCTG 
CCAGGTGATA CCTGTGACAG CCACGCTAGC GAGCTGGATG GCGCCCGGGA GGGGAAAGCG 
CTGCCAGGAA TCATCAAAGC CAGCGGCCCC TTGCCAGATG GTGCTTCTCT CAACAGTAGT 
ACCCTGTCAG ATGGGTCCCA GGATGAAGAA TGGAGTTTTA CTGTTCCCAG GAGTGGCTCC 
ATGTCCATCC TGGAGGCCAT TCCAGACGTG CCAGTTCATT CCAACGTCGC GGCAGACCCT 
GGACGGTCGG CGCGGAACGC TGCAAGTGAT GATGAATATC TGGAAAAAAA CATTCCGGTA 
GAAGCTGAAG AGCTGTCCTT TGAAGTATCA TATTCAGAAA AGCTAACACA GGCTCCAAAA 
AGGAACAGAT TTAAGAAGTT AGATTTTAAG AAAGAAGACT ATGTTTTACC TAAATTTGTT 
GTTCAGAAAA CCAGATTTGG CCTAACCAAA GCAGAAGATC TGTCTGCGGA AGACATGAAG 
AAAATCCGCC ATCTCTCTCT GATTGAATTG ACGGCCTTTT TTGATGCCTT TCGAATTCAG 
CTGAAAAGAA ACAAAACAGA GAAAGTCAAA GGACGAGACA GTGGGATTTT TGGAGTTCCC 
CTCACAGTCC TGCTAGAAAA TGACCGAAGG AAAGACTCTG GGGTCAAAGT TCCCCTTGTG 
TTACAAAAGT TCTTTGAGAA AGTTGAGGAA TCGGGTCTGG AATCTGAAGG GATCTTTCGA 
CTTTCAGGAT GTACTGCCAA AGTCAAGCAA TACCGTGAAG AACTGGATGC CAAGTTTAAT 
GCTGACAAGT TTAAATGGGA GAAAATGTGC CATAGAGAAG CTGCAGTGAT GCTGAAAGCC 
TTTTTCAGAG AACTGCCCAC CTCTCTCTTT CCCGTGGAAT ATATACCTGC CTTCATCACT 
CTAATGGAAA GAGGGCCTCA CATCCGAGTA CAGTTTCAAG CCTTACACCT CATGGTCATG 
GCACTGCCTG ATGCCAACAG GGACACAGCC CAGGCCCTCA TGACATTCTT CAATAAAGTC 
ATTGCCAATG AATCAAAAAA TCGCATGAGT ATATGGAACA TTTCTACTGT CATGGCACCA 
AATCTTTTCT TCAGTAGAAG CAAACATTCT GACTGTGAAG AATTACTCTT AGCAAACACT 
GCTGCCCACA TCATCCGTCT GATGCTCAAG TACCAGGAGA TGTTGTGGAC GGTTCCGTCT 
TTCTTAATCT CTCAAGTCAG AAGAATGAAT GAAGCCACCA TGTTGTTAAA GAAGCCGCTC 
CCAAGTGTCA AAAAGCTGCT AAGGAGGAAG ACCATAGAAC GAGGGGTTAC AAGCCCCAAG 
ACATCAAAGG TACTGCAAAA ATCACCCTCT ACAAGAAGAA TGTCCGACGT GCCAGAGGGC 
GTCATAAGGG TCCACGCGCC CCTTCTCTCC AAGGTGTCCA TGGCCATTCA ACTCAACAGT 
CAAACCAAAG TCAAAGACAT CCTGGCAAAA TTTCAATGTG AGAACAGTCA TGGTTCATCG 
GAATGCATTA AGATCCAGAA CCAAAGGTTA TATGAAATTG GAGGTAATAT AGGACAGCAC 
TGCTTGGATC CGGATGCTTA TATACTGGAT GTTTATCATG TGAATCCCCA GGCAGAATGG 
GTGATTAAAC CCCAACAGAG TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817249.1
CDS	566..2692
Translation	MLSNESLQSPAFSRSNSQASVDSASMENFWREIESIKETSLGAQEEQTPAEAKPLDEGELEAEWLQDVGLSTLISGDEEEDGKALLSTLTRTQAAAVKKRYNTYTQTLRKKNKQSVRDVRDIFGTSESPPDEFCCVPAPLLDVSPDEERMPTVPCRNGQLPGDTCDSHASELDGAREGKALPGIIKASGPLPDGASLNSSTLSDGSQDEEWSFTVPRSGSMSILEAIPDVPVHSNVAADPGRSARNAASDDEYLEKNIPVEAEELSFEVSYSEKLTQAPKRNRFKKLDFKKEDYVLPKFVVQKTRFGLTKAEDLSAEDMKKIRHLSLIELTAFFDAFRIQLKRNKTEKVKGRDSGIFGVPLTVLLENDRRKDSGVKVPLVLQKFFEKVEESGLESEGIFRLSGCTAKVKQYREELDAKFNADKFKWEKMCHREAAVMLKAFFRELPTSLFPVEYIPAFITLMERGPHIRVQFQALHLMVMALPDANRDTAQALMTFFNKVIANESKNRMSIWNISTVMAPNLFFSRSKHSDCEELLLANTAAHIIRLMLKYQEMLWTVPSFLISQVRRMNEATMLLKKPLPSVKKLLRRKTIERGVTSPKTSKVLQKSPSTRRMSDVPEGVIRVHAPLLSKVSMAIQLNSQTKVKDILAKFQCENSHGSSECIKIQNQRLYEIGGNIGQHCLDPDAYILDVYHVNPQAEWVIKPQQSV

Target ORF information:

RefSeq Version	XM_010818947.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818947.3

ORF Insert Sequence:

ATGCTCTCCA ATGAGTCCCT TCAGTCCCCG GCGTTCAGCC GCTCCAACTC TCAAGCCTCC 
GTAGACAGCG CCTCCATGGA GAATTTCTGG CGGGAAATAG AAAGCATCAA AGAGACCAGC 
CTCGGGGCTC AGGAGGAACA GACGCCTGCC GAGGCCAAGC CCCTGGACGA AGGAGAACTT 
GAAGCAGAGT GGTTGCAAGA TGTTGGTTTA TCAACCCTGA TCTCGGGTGA TGAGGAGGAA 
GATGGCAAAG CCTTGCTCTC TACTTTGACT CGAACCCAGG CAGCTGCAGT GAAGAAGAGA 
TACAATACTT ACACACAAAC CTTGAGGAAA AAGAACAAGC AGTCTGTCAG GGATGTCAGA 
GACATCTTCG GAACCAGCGA ATCTCCTCCA GATGAGTTTT GCTGTGTCCC AGCTCCTCTT 
CTGGATGTGA GCCCGGATGA AGAAAGGATG CCAACGGTCC CTTGCAGAAA TGGTCAGCTG 
CCAGGTGATA CCTGTGACAG CCACGCTAGC GAGCTGGATG GCGCCCGGGA GGGGAAAGCG 
CTGCCAGGAA TCATCAAAGC CAGCGGCCCC TTGCCAGATG GTGCTTCTCT CAACAGTAGT 
ACCCTGTCAG ATGGGTCCCA GGATGAAGAA TGGAGTTTTA CTGTTCCCAG GAGTGGCTCC 
ATGTCCATCC TGGAGGCCAT TCCAGACGTG CCAGTTCATT CCAACGTCGC GGCAGACCCT 
GGACGGTCGG CGCGGAACGC TGCAAGTGAT GATGAATATC TGGAAAAAAA CATTCCGGTA 
GAAGCTGAAG AGCTGTCCTT TGAAGTATCA TATTCAGAAA AGCTAACACA GGCTCCAAAA 
AGGAACAGAT TTAAGAAGTT AGATTTTAAG AAAGAAGACT ATGTTTTACC TAAATTTGTT 
GTTCAGAAAA CCAGATTTGG CCTAACCAAA GCAGAAGATC TGTCTGCGGA AGACATGAAG 
AAAATCCGCC ATCTCTCTCT GATTGAATTG ACGGCCTTTT TTGATGCCTT TCGAATTCAG 
CTGAAAAGAA ACAAAACAGA GAAAGTCAAA GGACGAGACA GTGGGATTTT TGGAGTTCCC 
CTCACAGTCC TGCTAGAAAA TGACCGAAGG AAAGACTCTG GGGTCAAAGT TCCCCTTGTG 
TTACAAAAGT TCTTTGAGAA AGTTGAGGAA TCGGGTCTGG AATCTGAAGG GATCTTTCGA 
CTTTCAGGAT GTACTGCCAA AGTCAAGCAA TACCGTGAAG AACTGGATGC CAAGTTTAAT 
GCTGACAAGT TTAAATGGGA GAAAATGTGC CATAGAGAAG CTGCAGTGAT GCTGAAAGCC 
TTTTTCAGAG AACTGCCCAC CTCTCTCTTT CCCGTGGAAT ATATACCTGC CTTCATCACT 
CTAATGGAAA GAGGGCCTCA CATCCGAGTA CAGTTTCAAG CCTTACACCT CATGGTCATG 
GCACTGCCTG ATGCCAACAG GGACACAGCC CAGGCCCTCA TGACATTCTT CAATAAAGTC 
ATTGCCAATG AATCAAAAAA TCGCATGAGT ATATGGAACA TTTCTACTGT CATGGCACCA 
AATCTTTTCT TCAGTAGAAG CAAACATTCT GACTGTGAAG AATTACTCTT AGCAAACACT 
GCTGCCCACA TCATCCGTCT GATGCTCAAG TACCAGGAGA TGTTGTGGAC GGTTCCGTCT 
TTCTTAATCT CTCAAGTCAG AAGAATGAAT GAAGCCACCA TGTTGTTAAA GAAGCCGCTC 
CCAAGTGTCA AAAAGCTGCT AAGGAGGAAG ACCATAGAAC GAGGGGTTAC AAGCCCCAAG 
ACATCAAAGG TACTGCAAAA ATCACCCTCT ACAAGAAGAA TGTCCGACGT GCCAGAGGGC 
GTCATAAGGG TCCACGCGCC CCTTCTCTCC AAGGTGTCCA TGGCCATTCA ACTCAACAGT 
CAAACCAAAG TCAAAGACAT CCTGGCAAAA TTTCAATGTG AGAACAGTCA TGGTTCATCG 
GAATGCATTA AGATCCAGAA CCAAAGGTTA TATGAAATTG GAGGTAATAT AGGACAGCAC 
TGCTTGGATC CGGATGCTTA TATACTGGAT GTTTATCATG TGAATCCCCA GGCAGAATGG 
GTGATTAAAC CCCAACAGAG TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP28 ( XM_010818945.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817247.1 Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa51832
Clone ID Related Accession (Same CDS sequence)	XM_010818945.2 , XM_010825549.2
Accession Version	XM_010818945.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTCC CCGCCTTTGT TCTCCGGCCG GAGCGGAGCC ATGCGCGGCT GACGATGGAA 
GTGGAGGACT CGGGCGGCGT GGTGCTCACC GCCTACCACT CGTACGCGCG CCCCCCGCCG 
CCCAGCCCGG AGCCGCGCTG CGCGCCCCGG GCGACCGCCA GCCTCCCCGG CAGCAGAAAG 
TCCATCCCTC GCTGCCGAAG AATCAACAGG ATGCTCTCCA ATGAGTCCCT TCAGTCCCCG 
GCGTTCAGCC GCTCCAACTC TCAAGCCTCC GTAGACAGCG CCTCCATGGA GAATTTCTGG 
CGGGAAATAG AAAGCATCAA AGAGACCAGC CTCGGGGCTC AGGAGGAACA GACGCCTGCC 
GAGGCCAAGC CCCTGGACGA AGGAGAACTT GAAGCAGAGT GGTTGCAAGA TGTTGGTTTA 
TCAACCCTGA TCTCGGGTGA TGAGGAGGAA GATGGCAAAG CCTTGCTCTC TACTTTGACT 
CGAACCCAGG CAGCTGCAGT GAAGAAGAGA TACAATACTT ACACACAAAC CTTGAGGAAA 
AAGAACAAGC AGTCTGTCAG GGATGTCAGA GACATCTTCG GAACCAGCGA ATCTCCTCCA 
GATGAGTTTT GCTGTGTCCC AGCTCCTCTT CTGGATGTGA GCCCGGATGA AGAAAGGATG 
CCAACGGTCC CTTGCAGAAA TGGTCAGCTG CCAGGTGATA CCTGTGACAG CCACGCTAGC 
GAGCTGGATG GCGCCCGGGA GGGGAAAGCG CTGCCAGGAA TCATCAAAGC CAGCGGCCCC 
TTGCCAGATG GTGCTTCTCT CAACAGTAGT ACCCTGTCAG ATGGGTCCCA GGATGAAGAA 
TGGAGTTTTA CTGTTCCCAG GAGTGGCTCC ATGTCCATCC TGGAGGCCAT TCCAGACGTG 
CCAGTTCATT CCAACGTCGC GGCAGACCCT GGACGGTCGG CGCGGAACGC TGCAAGTGAT 
GATGAATATC TGGAAAAAAA CATTCCGGTA GAAGCTGAAG AGCTGTCCTT TGAAGTATCA 
TATTCAGAAA AGCTAACACA GGCTCCAAAA AGGAACAGAT TTAAGAAGTT AGATTTTAAG 
AAAGAAGACT ATGTTTTACC TAAATTTGTT GTTCAGAAAA CCAGATTTGG CCTAACCAAA 
GCAGAAGATC TGTCTGCGGA AGACATGAAG AAAATCCGCC ATCTCTCTCT GATTGAATTG 
ACGGCCTTTT TTGATGCCTT TCGAATTCAG CTGAAAAGAA ACAAAACAGA GAAAGTCAAA 
GGACGAGACA GTGGGATTTT TGGAGTTCCC CTCACAGTCC TGCTAGAAAA TGACCGAAGG 
AAAGACTCTG GGGTCAAAGT TCCCCTTGTG TTACAAAAGT TCTTTGAGAA AGTTGAGGAA 
TCGGGTCTGG AATCTGAAGG GATCTTTCGA CTTTCAGGAT GTACTGCCAA AGTCAAGCAA 
TACCGTGAAG AACTGGATGC CAAGTTTAAT GCTGACAAGT TTAAATGGGA GAAAATGTGC 
CATAGAGAAG CTGCAGTGAT GCTGAAAGCC TTTTTCAGAG AACTGCCCAC CTCTCTCTTT 
CCCGTGGAAT ATATACCTGC CTTCATCACT CTAATGGAAA GAGGGCCTCA CATCCGAGTA 
CAGTTTCAAG CCTTACACCT CATGGTCATG GCACTGCCTG ATGCCAACAG GGACACAGCC 
CAGGCCCTCA TGACATTCTT CAATAAAGTC ATTGCCAATG AATCAAAAAA TCGCATGAGT 
ATATGGAACA TTTCTACTGT CATGGCACCC AATCTTTTCT TCAGTAGAAG CAAACATTCT 
GACTGTGAAG AATTACTCTT AGCAAACACT GCTGCCCACA TCATCCGTCT GATGCTCAAG 
TACCAGGAGA TGTTGTGGAC GGTTCCGTCT TTCTTAATCT CTCAAGTCAG AAGAATGAAT 
GAAGCCACCA TGTTGTTAAA GAAGCCGCTC CCAAGTGTCA AAAAGCTGCT AAGGAGGAAG 
ACCATAGAAC GAGGGGTTAC AAGCCCCAAG ACATCAAAGG TACTGCAAAA ATCACCCTCT 
ACAAGAAGAA TGTCCGACGT GCCAGAGGGC GTCATAAGGG TCCACGCGCC CCTTCTCTCC 
AAGGTGTCCA TGGCCATTCA ACTCAACAGT CAAACCAAAG TCAAAGACAT CCTGGCAAAA 
TTTCAATGTG AGAACAGTCA TGGTTCATCG GAATGCATTA AGATCCAGAA CCAAAGGTTA 
TATGAAATTG GAGGTAATAT AGGACAGCAC TGCTTGGATC CGGATGCTTA TATACTGGAT 
GTTTATCATG TGAATCCCCA GGCAGAATGG GTGATTAAAC CCCAACAGAG TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817247.1
CDS	33..2369
Translation	MLVPAFVLRPERSHARLTMEVEDSGGVVLTAYHSYARPPPPSPEPRCAPRATASLPGSRKSIPRCRRINRMLSNESLQSPAFSRSNSQASVDSASMENFWREIESIKETSLGAQEEQTPAEAKPLDEGELEAEWLQDVGLSTLISGDEEEDGKALLSTLTRTQAAAVKKRYNTYTQTLRKKNKQSVRDVRDIFGTSESPPDEFCCVPAPLLDVSPDEERMPTVPCRNGQLPGDTCDSHASELDGAREGKALPGIIKASGPLPDGASLNSSTLSDGSQDEEWSFTVPRSGSMSILEAIPDVPVHSNVAADPGRSARNAASDDEYLEKNIPVEAEELSFEVSYSEKLTQAPKRNRFKKLDFKKEDYVLPKFVVQKTRFGLTKAEDLSAEDMKKIRHLSLIELTAFFDAFRIQLKRNKTEKVKGRDSGIFGVPLTVLLENDRRKDSGVKVPLVLQKFFEKVEESGLESEGIFRLSGCTAKVKQYREELDAKFNADKFKWEKMCHREAAVMLKAFFRELPTSLFPVEYIPAFITLMERGPHIRVQFQALHLMVMALPDANRDTAQALMTFFNKVIANESKNRMSIWNISTVMAPNLFFSRSKHSDCEELLLANTAAHIIRLMLKYQEMLWTVPSFLISQVRRMNEATMLLKKPLPSVKKLLRRKTIERGVTSPKTSKVLQKSPSTRRMSDVPEGVIRVHAPLLSKVSMAIQLNSQTKVKDILAKFQCENSHGSSECIKIQNQRLYEIGGNIGQHCLDPDAYILDVYHVNPQAEWVIKPQQSV

Target ORF information:

RefSeq Version	XM_010818945.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818945.2

ORF Insert Sequence:

ATGTTGGTCC CCGCCTTTGT TCTCCGGCCG GAGCGGAGCC ATGCGCGGCT GACGATGGAA 
GTGGAGGACT CGGGCGGCGT GGTGCTCACC GCCTACCACT CGTACGCGCG CCCCCCGCCG 
CCCAGCCCGG AGCCGCGCTG CGCGCCCCGG GCGACCGCCA GCCTCCCCGG CAGCAGAAAG 
TCCATCCCTC GCTGCCGAAG AATCAACAGG ATGCTCTCCA ATGAGTCCCT TCAGTCCCCG 
GCGTTCAGCC GCTCCAACTC TCAAGCCTCC GTAGACAGCG CCTCCATGGA GAATTTCTGG 
CGGGAAATAG AAAGCATCAA AGAGACCAGC CTCGGGGCTC AGGAGGAACA GACGCCTGCC 
GAGGCCAAGC CCCTGGACGA AGGAGAACTT GAAGCAGAGT GGTTGCAAGA TGTTGGTTTA 
TCAACCCTGA TCTCGGGTGA TGAGGAGGAA GATGGCAAAG CCTTGCTCTC TACTTTGACT 
CGAACCCAGG CAGCTGCAGT GAAGAAGAGA TACAATACTT ACACACAAAC CTTGAGGAAA 
AAGAACAAGC AGTCTGTCAG GGATGTCAGA GACATCTTCG GAACCAGCGA ATCTCCTCCA 
GATGAGTTTT GCTGTGTCCC AGCTCCTCTT CTGGATGTGA GCCCGGATGA AGAAAGGATG 
CCAACGGTCC CTTGCAGAAA TGGTCAGCTG CCAGGTGATA CCTGTGACAG CCACGCTAGC 
GAGCTGGATG GCGCCCGGGA GGGGAAAGCG CTGCCAGGAA TCATCAAAGC CAGCGGCCCC 
TTGCCAGATG GTGCTTCTCT CAACAGTAGT ACCCTGTCAG ATGGGTCCCA GGATGAAGAA 
TGGAGTTTTA CTGTTCCCAG GAGTGGCTCC ATGTCCATCC TGGAGGCCAT TCCAGACGTG 
CCAGTTCATT CCAACGTCGC GGCAGACCCT GGACGGTCGG CGCGGAACGC TGCAAGTGAT 
GATGAATATC TGGAAAAAAA CATTCCGGTA GAAGCTGAAG AGCTGTCCTT TGAAGTATCA 
TATTCAGAAA AGCTAACACA GGCTCCAAAA AGGAACAGAT TTAAGAAGTT AGATTTTAAG 
AAAGAAGACT ATGTTTTACC TAAATTTGTT GTTCAGAAAA CCAGATTTGG CCTAACCAAA 
GCAGAAGATC TGTCTGCGGA AGACATGAAG AAAATCCGCC ATCTCTCTCT GATTGAATTG 
ACGGCCTTTT TTGATGCCTT TCGAATTCAG CTGAAAAGAA ACAAAACAGA GAAAGTCAAA 
GGACGAGACA GTGGGATTTT TGGAGTTCCC CTCACAGTCC TGCTAGAAAA TGACCGAAGG 
AAAGACTCTG GGGTCAAAGT TCCCCTTGTG TTACAAAAGT TCTTTGAGAA AGTTGAGGAA 
TCGGGTCTGG AATCTGAAGG GATCTTTCGA CTTTCAGGAT GTACTGCCAA AGTCAAGCAA 
TACCGTGAAG AACTGGATGC CAAGTTTAAT GCTGACAAGT TTAAATGGGA GAAAATGTGC 
CATAGAGAAG CTGCAGTGAT GCTGAAAGCC TTTTTCAGAG AACTGCCCAC CTCTCTCTTT 
CCCGTGGAAT ATATACCTGC CTTCATCACT CTAATGGAAA GAGGGCCTCA CATCCGAGTA 
CAGTTTCAAG CCTTACACCT CATGGTCATG GCACTGCCTG ATGCCAACAG GGACACAGCC 
CAGGCCCTCA TGACATTCTT CAATAAAGTC ATTGCCAATG AATCAAAAAA TCGCATGAGT 
ATATGGAACA TTTCTACTGT CATGGCACCC AATCTTTTCT TCAGTAGAAG CAAACATTCT 
GACTGTGAAG AATTACTCTT AGCAAACACT GCTGCCCACA TCATCCGTCT GATGCTCAAG 
TACCAGGAGA TGTTGTGGAC GGTTCCGTCT TTCTTAATCT CTCAAGTCAG AAGAATGAAT 
GAAGCCACCA TGTTGTTAAA GAAGCCGCTC CCAAGTGTCA AAAAGCTGCT AAGGAGGAAG 
ACCATAGAAC GAGGGGTTAC AAGCCCCAAG ACATCAAAGG TACTGCAAAA ATCACCCTCT 
ACAAGAAGAA TGTCCGACGT GCCAGAGGGC GTCATAAGGG TCCACGCGCC CCTTCTCTCC 
AAGGTGTCCA TGGCCATTCA ACTCAACAGT CAAACCAAAG TCAAAGACAT CCTGGCAAAA 
TTTCAATGTG AGAACAGTCA TGGTTCATCG GAATGCATTA AGATCCAGAA CCAAAGGTTA 
TATGAAATTG GAGGTAATAT AGGACAGCAC TGCTTGGATC CGGATGCTTA TATACTGGAT 
GTTTATCATG TGAATCCCCA GGCAGAATGG GTGATTAAAC CCCAACAGAG TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP28 ( XM_010825549.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823851.2 Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa51832
Clone ID Related Accession (Same CDS sequence)	XM_010818945.2 , XM_010825549.2
Accession Version	XM_010825549.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTCC CCGCCTTTGT TCTCCGGCCG GAGCGGAGCC ATGCGCGGCT GACGATGGAA 
GTGGAGGACT CGGGCGGCGT GGTGCTCACC GCCTACCACT CGTACGCGCG CCCCCCGCCG 
CCCAGCCCGG AGCCGCGCTG CGCGCCCCGG GCGACCGCCA GCCTCCCCGG CAGCAGAAAG 
TCCATCCCTC GCTGCCGAAG AATCAACAGG ATGCTCTCCA ATGAGTCCCT TCAGTCCCCG 
GCGTTCAGCC GCTCCAACTC TCAAGCCTCC GTAGACAGCG CCTCCATGGA GAATTTCTGG 
CGGGAAATAG AAAGCATCAA AGAGACCAGC CTCGGGGCTC AGGAGGAACA GACGCCTGCC 
GAGGCCAAGC CCCTGGACGA AGGAGAACTT GAAGCAGAGT GGTTGCAAGA TGTTGGTTTA 
TCAACCCTGA TCTCGGGTGA TGAGGAGGAA GATGGCAAAG CCTTGCTCTC TACTTTGACT 
CGAACCCAGG CAGCTGCAGT GAAGAAGAGA TACAATACTT ACACACAAAC CTTGAGGAAA 
AAGAACAAGC AGTCTGTCAG GGATGTCAGA GACATCTTCG GAACCAGCGA ATCTCCTCCA 
GATGAGTTTT GCTGTGTCCC AGCTCCTCTT CTGGATGTGA GCCCGGATGA AGAAAGGATG 
CCAACGGTCC CTTGCAGAAA TGGTCAGCTG CCAGGTGATA CCTGTGACAG CCACGCTAGC 
GAGCTGGATG GCGCCCGGGA GGGGAAAGCG CTGCCAGGAA TCATCAAAGC CAGCGGCCCC 
TTGCCAGATG GTGCTTCTCT CAACAGTAGT ACCCTGTCAG ATGGGTCCCA GGATGAAGAA 
TGGAGTTTTA CTGTTCCCAG GAGTGGCTCC ATGTCCATCC TGGAGGCCAT TCCAGACGTG 
CCAGTTCATT CCAACGTCGC GGCAGACCCT GGACGGTCGG CGCGGAACGC TGCAAGTGAT 
GATGAATATC TGGAAAAAAA CATTCCGGTA GAAGCTGAAG AGCTGTCCTT TGAAGTATCA 
TATTCAGAAA AGCTAACACA GGCTCCAAAA AGGAACAGAT TTAAGAAGTT AGATTTTAAG 
AAAGAAGACT ATGTTTTACC TAAATTTGTT GTTCAGAAAA CCAGATTTGG CCTAACCAAA 
GCAGAAGATC TGTCTGCGGA AGACATGAAG AAAATCCGCC ATCTCTCTCT GATTGAATTG 
ACGGCCTTTT TTGATGCCTT TCGAATTCAG CTGAAAAGAA ACAAAACAGA GAAAGTCAAA 
GGACGAGACA GTGGGATTTT TGGAGTTCCC CTCACAGTCC TGCTAGAAAA TGACCGAAGG 
AAAGACTCTG GGGTCAAAGT TCCCCTTGTG TTACAAAAGT TCTTTGAGAA AGTTGAGGAA 
TCGGGTCTGG AATCTGAAGG GATCTTTCGA CTTTCAGGAT GTACTGCCAA AGTCAAGCAA 
TACCGTGAAG AACTGGATGC CAAGTTTAAT GCTGACAAGT TTAAATGGGA GAAAATGTGC 
CATAGAGAAG CTGCAGTGAT GCTGAAAGCC TTTTTCAGAG AACTGCCCAC CTCTCTCTTT 
CCCGTGGAAT ATATACCTGC CTTCATCACT CTAATGGAAA GAGGGCCTCA CATCCGAGTA 
CAGTTTCAAG CCTTACACCT CATGGTCATG GCACTGCCTG ATGCCAACAG GGACACAGCC 
CAGGCCCTCA TGACATTCTT CAATAAAGTC ATTGCCAATG AATCAAAAAA TCGCATGAGT 
ATATGGAACA TTTCTACTGT CATGGCACCC AATCTTTTCT TCAGTAGAAG CAAACATTCT 
GACTGTGAAG AATTACTCTT AGCAAACACT GCTGCCCACA TCATCCGTCT GATGCTCAAG 
TACCAGGAGA TGTTGTGGAC GGTTCCGTCT TTCTTAATCT CTCAAGTCAG AAGAATGAAT 
GAAGCCACCA TGTTGTTAAA GAAGCCGCTC CCAAGTGTCA AAAAGCTGCT AAGGAGGAAG 
ACCATAGAAC GAGGGGTTAC AAGCCCCAAG ACATCAAAGG TACTGCAAAA ATCACCCTCT 
ACAAGAAGAA TGTCCGACGT GCCAGAGGGC GTCATAAGGG TCCACGCGCC CCTTCTCTCC 
AAGGTGTCCA TGGCCATTCA ACTCAACAGT CAAACCAAAG TCAAAGACAT CCTGGCAAAA 
TTTCAATGTG AGAACAGTCA TGGTTCATCG GAATGCATTA AGATCCAGAA CCAAAGGTTA 
TATGAAATTG GAGGTAATAT AGGACAGCAC TGCTTGGATC CGGATGCTTA TATACTGGAT 
GTTTATCATG TGAATCCCCA GGCAGAATGG GTGATTAAAC CCCAACAGAG TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823851.2
CDS	33..2369
Translation	MLVPAFVLRPERSHARLTMEVEDSGGVVLTAYHSYARPPPPSPEPRCAPRATASLPGSRKSIPRCRRINRMLSNESLQSPAFSRSNSQASVDSASMENFWREIESIKETSLGAQEEQTPAEAKPLDEGELEAEWLQDVGLSTLISGDEEEDGKALLSTLTRTQAAAVKKRYNTYTQTLRKKNKQSVRDVRDIFGTSESPPDEFCCVPAPLLDVSPDEERMPTVPCRNGQLPGDTCDSHASELDGAREGKALPGIIKASGPLPDGASLNSSTLSDGSQDEEWSFTVPRSGSMSILEAIPDVPVHSNVAADPGRSARNAASDDEYLEKNIPVEAEELSFEVSYSEKLTQAPKRNRFKKLDFKKEDYVLPKFVVQKTRFGLTKAEDLSAEDMKKIRHLSLIELTAFFDAFRIQLKRNKTEKVKGRDSGIFGVPLTVLLENDRRKDSGVKVPLVLQKFFEKVEESGLESEGIFRLSGCTAKVKQYREELDAKFNADKFKWEKMCHREAAVMLKAFFRELPTSLFPVEYIPAFITLMERGPHIRVQFQALHLMVMALPDANRDTAQALMTFFNKVIANESKNRMSIWNISTVMAPNLFFSRSKHSDCEELLLANTAAHIIRLMLKYQEMLWTVPSFLISQVRRMNEATMLLKKPLPSVKKLLRRKTIERGVTSPKTSKVLQKSPSTRRMSDVPEGVIRVHAPLLSKVSMAIQLNSQTKVKDILAKFQCENSHGSSECIKIQNQRLYEIGGNIGQHCLDPDAYILDVYHVNPQAEWVIKPQQSV

Target ORF information:

RefSeq Version	XM_010825549.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825549.2

ORF Insert Sequence:

ATGTTGGTCC CCGCCTTTGT TCTCCGGCCG GAGCGGAGCC ATGCGCGGCT GACGATGGAA 
GTGGAGGACT CGGGCGGCGT GGTGCTCACC GCCTACCACT CGTACGCGCG CCCCCCGCCG 
CCCAGCCCGG AGCCGCGCTG CGCGCCCCGG GCGACCGCCA GCCTCCCCGG CAGCAGAAAG 
TCCATCCCTC GCTGCCGAAG AATCAACAGG ATGCTCTCCA ATGAGTCCCT TCAGTCCCCG 
GCGTTCAGCC GCTCCAACTC TCAAGCCTCC GTAGACAGCG CCTCCATGGA GAATTTCTGG 
CGGGAAATAG AAAGCATCAA AGAGACCAGC CTCGGGGCTC AGGAGGAACA GACGCCTGCC 
GAGGCCAAGC CCCTGGACGA AGGAGAACTT GAAGCAGAGT GGTTGCAAGA TGTTGGTTTA 
TCAACCCTGA TCTCGGGTGA TGAGGAGGAA GATGGCAAAG CCTTGCTCTC TACTTTGACT 
CGAACCCAGG CAGCTGCAGT GAAGAAGAGA TACAATACTT ACACACAAAC CTTGAGGAAA 
AAGAACAAGC AGTCTGTCAG GGATGTCAGA GACATCTTCG GAACCAGCGA ATCTCCTCCA 
GATGAGTTTT GCTGTGTCCC AGCTCCTCTT CTGGATGTGA GCCCGGATGA AGAAAGGATG 
CCAACGGTCC CTTGCAGAAA TGGTCAGCTG CCAGGTGATA CCTGTGACAG CCACGCTAGC 
GAGCTGGATG GCGCCCGGGA GGGGAAAGCG CTGCCAGGAA TCATCAAAGC CAGCGGCCCC 
TTGCCAGATG GTGCTTCTCT CAACAGTAGT ACCCTGTCAG ATGGGTCCCA GGATGAAGAA 
TGGAGTTTTA CTGTTCCCAG GAGTGGCTCC ATGTCCATCC TGGAGGCCAT TCCAGACGTG 
CCAGTTCATT CCAACGTCGC GGCAGACCCT GGACGGTCGG CGCGGAACGC TGCAAGTGAT 
GATGAATATC TGGAAAAAAA CATTCCGGTA GAAGCTGAAG AGCTGTCCTT TGAAGTATCA 
TATTCAGAAA AGCTAACACA GGCTCCAAAA AGGAACAGAT TTAAGAAGTT AGATTTTAAG 
AAAGAAGACT ATGTTTTACC TAAATTTGTT GTTCAGAAAA CCAGATTTGG CCTAACCAAA 
GCAGAAGATC TGTCTGCGGA AGACATGAAG AAAATCCGCC ATCTCTCTCT GATTGAATTG 
ACGGCCTTTT TTGATGCCTT TCGAATTCAG CTGAAAAGAA ACAAAACAGA GAAAGTCAAA 
GGACGAGACA GTGGGATTTT TGGAGTTCCC CTCACAGTCC TGCTAGAAAA TGACCGAAGG 
AAAGACTCTG GGGTCAAAGT TCCCCTTGTG TTACAAAAGT TCTTTGAGAA AGTTGAGGAA 
TCGGGTCTGG AATCTGAAGG GATCTTTCGA CTTTCAGGAT GTACTGCCAA AGTCAAGCAA 
TACCGTGAAG AACTGGATGC CAAGTTTAAT GCTGACAAGT TTAAATGGGA GAAAATGTGC 
CATAGAGAAG CTGCAGTGAT GCTGAAAGCC TTTTTCAGAG AACTGCCCAC CTCTCTCTTT 
CCCGTGGAAT ATATACCTGC CTTCATCACT CTAATGGAAA GAGGGCCTCA CATCCGAGTA 
CAGTTTCAAG CCTTACACCT CATGGTCATG GCACTGCCTG ATGCCAACAG GGACACAGCC 
CAGGCCCTCA TGACATTCTT CAATAAAGTC ATTGCCAATG AATCAAAAAA TCGCATGAGT 
ATATGGAACA TTTCTACTGT CATGGCACCC AATCTTTTCT TCAGTAGAAG CAAACATTCT 
GACTGTGAAG AATTACTCTT AGCAAACACT GCTGCCCACA TCATCCGTCT GATGCTCAAG 
TACCAGGAGA TGTTGTGGAC GGTTCCGTCT TTCTTAATCT CTCAAGTCAG AAGAATGAAT 
GAAGCCACCA TGTTGTTAAA GAAGCCGCTC CCAAGTGTCA AAAAGCTGCT AAGGAGGAAG 
ACCATAGAAC GAGGGGTTAC AAGCCCCAAG ACATCAAAGG TACTGCAAAA ATCACCCTCT 
ACAAGAAGAA TGTCCGACGT GCCAGAGGGC GTCATAAGGG TCCACGCGCC CCTTCTCTCC 
AAGGTGTCCA TGGCCATTCA ACTCAACAGT CAAACCAAAG TCAAAGACAT CCTGGCAAAA 
TTTCAATGTG AGAACAGTCA TGGTTCATCG GAATGCATTA AGATCCAGAA CCAAAGGTTA 
TATGAAATTG GAGGTAATAT AGGACAGCAC TGCTTGGATC CGGATGCTTA TATACTGGAT 
GTTTATCATG TGAATCCCCA GGCAGAATGG GTGATTAAAC CCCAACAGAG TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP28 ( XM_010825551.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823853.2 Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa51833
Clone ID Related Accession (Same CDS sequence)	XM_010825551.2 , XM_010818947.2
Accession Version	XM_010825551.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCA ATGAGTCCCT TCAGTCCCCG GCGTTCAGCC GCTCCAACTC TCAAGCCTCC 
GTAGACAGCG CCTCCATGGA GAATTTCTGG CGGGAAATAG AAAGCATCAA AGAGACCAGC 
CTCGGGGCTC AGGAGGAACA GACGCCTGCC GAGGCCAAGC CCCTGGACGA AGGAGAACTT 
GAAGCAGAGT GGTTGCAAGA TGTTGGTTTA TCAACCCTGA TCTCGGGTGA TGAGGAGGAA 
GATGGCAAAG CCTTGCTCTC TACTTTGACT CGAACCCAGG CAGCTGCAGT GAAGAAGAGA 
TACAATACTT ACACACAAAC CTTGAGGAAA AAGAACAAGC AGTCTGTCAG GGATGTCAGA 
GACATCTTCG GAACCAGCGA ATCTCCTCCA GATGAGTTTT GCTGTGTCCC AGCTCCTCTT 
CTGGATGTGA GCCCGGATGA AGAAAGGATG CCAACGGTCC CTTGCAGAAA TGGTCAGCTG 
CCAGGTGATA CCTGTGACAG CCACGCTAGC GAGCTGGATG GCGCCCGGGA GGGGAAAGCG 
CTGCCAGGAA TCATCAAAGC CAGCGGCCCC TTGCCAGATG GTGCTTCTCT CAACAGTAGT 
ACCCTGTCAG ATGGGTCCCA GGATGAAGAA TGGAGTTTTA CTGTTCCCAG GAGTGGCTCC 
ATGTCCATCC TGGAGGCCAT TCCAGACGTG CCAGTTCATT CCAACGTCGC GGCAGACCCT 
GGACGGTCGG CGCGGAACGC TGCAAGTGAT GATGAATATC TGGAAAAAAA CATTCCGGTA 
GAAGCTGAAG AGCTGTCCTT TGAAGTATCA TATTCAGAAA AGCTAACACA GGCTCCAAAA 
AGGAACAGAT TTAAGAAGTT AGATTTTAAG AAAGAAGACT ATGTTTTACC TAAATTTGTT 
GTTCAGAAAA CCAGATTTGG CCTAACCAAA GCAGAAGATC TGTCTGCGGA AGACATGAAG 
AAAATCCGCC ATCTCTCTCT GATTGAATTG ACGGCCTTTT TTGATGCCTT TCGAATTCAG 
CTGAAAAGAA ACAAAACAGA GAAAGTCAAA GGACGAGACA GTGGGATTTT TGGAGTTCCC 
CTCACAGTCC TGCTAGAAAA TGACCGAAGG AAAGACTCTG GGGTCAAAGT TCCCCTTGTG 
TTACAAAAGT TCTTTGAGAA AGTTGAGGAA TCGGGTCTGG AATCTGAAGG GATCTTTCGA 
CTTTCAGGAT GTACTGCCAA AGTCAAGCAA TACCGTGAAG AACTGGATGC CAAGTTTAAT 
GCTGACAAGT TTAAATGGGA GAAAATGTGC CATAGAGAAG CTGCAGTGAT GCTGAAAGCC 
TTTTTCAGAG AACTGCCCAC CTCTCTCTTT CCCGTGGAAT ATATACCTGC CTTCATCACT 
CTAATGGAAA GAGGGCCTCA CATCCGAGTA CAGTTTCAAG CCTTACACCT CATGGTCATG 
GCACTGCCTG ATGCCAACAG GGACACAGCC CAGGCCCTCA TGACATTCTT CAATAAAGTC 
ATTGCCAATG AATCAAAAAA TCGCATGAGT ATATGGAACA TTTCTACTGT CATGGCACCC 
AATCTTTTCT TCAGTAGAAG CAAACATTCT GACTGTGAAG AATTACTCTT AGCAAACACT 
GCTGCCCACA TCATCCGTCT GATGCTCAAG TACCAGGAGA TGTTGTGGAC GGTTCCGTCT 
TTCTTAATCT CTCAAGTCAG AAGAATGAAT GAAGCCACCA TGTTGTTAAA GAAGCCGCTC 
CCAAGTGTCA AAAAGCTGCT AAGGAGGAAG ACCATAGAAC GAGGGGTTAC AAGCCCCAAG 
ACATCAAAGG TACTGCAAAA ATCACCCTCT ACAAGAAGAA TGTCCGACGT GCCAGAGGGC 
GTCATAAGGG TCCACGCGCC CCTTCTCTCC AAGGTGTCCA TGGCCATTCA ACTCAACAGT 
CAAACCAAAG TCAAAGACAT CCTGGCAAAA TTTCAATGTG AGAACAGTCA TGGTTCATCG 
GAATGCATTA AGATCCAGAA CCAAAGGTTA TATGAAATTG GAGGTAATAT AGGACAGCAC 
TGCTTGGATC CGGATGCTTA TATACTGGAT GTTTATCATG TGAATCCCCA GGCAGAATGG 
GTGATTAAAC CCCAACAGAG TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823853.2
CDS	1147..3273
Translation	MLSNESLQSPAFSRSNSQASVDSASMENFWREIESIKETSLGAQEEQTPAEAKPLDEGELEAEWLQDVGLSTLISGDEEEDGKALLSTLTRTQAAAVKKRYNTYTQTLRKKNKQSVRDVRDIFGTSESPPDEFCCVPAPLLDVSPDEERMPTVPCRNGQLPGDTCDSHASELDGAREGKALPGIIKASGPLPDGASLNSSTLSDGSQDEEWSFTVPRSGSMSILEAIPDVPVHSNVAADPGRSARNAASDDEYLEKNIPVEAEELSFEVSYSEKLTQAPKRNRFKKLDFKKEDYVLPKFVVQKTRFGLTKAEDLSAEDMKKIRHLSLIELTAFFDAFRIQLKRNKTEKVKGRDSGIFGVPLTVLLENDRRKDSGVKVPLVLQKFFEKVEESGLESEGIFRLSGCTAKVKQYREELDAKFNADKFKWEKMCHREAAVMLKAFFRELPTSLFPVEYIPAFITLMERGPHIRVQFQALHLMVMALPDANRDTAQALMTFFNKVIANESKNRMSIWNISTVMAPNLFFSRSKHSDCEELLLANTAAHIIRLMLKYQEMLWTVPSFLISQVRRMNEATMLLKKPLPSVKKLLRRKTIERGVTSPKTSKVLQKSPSTRRMSDVPEGVIRVHAPLLSKVSMAIQLNSQTKVKDILAKFQCENSHGSSECIKIQNQRLYEIGGNIGQHCLDPDAYILDVYHVNPQAEWVIKPQQSV

Target ORF information:

RefSeq Version	XM_010825551.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825551.2

ORF Insert Sequence:

ATGCTCTCCA ATGAGTCCCT TCAGTCCCCG GCGTTCAGCC GCTCCAACTC TCAAGCCTCC 
GTAGACAGCG CCTCCATGGA GAATTTCTGG CGGGAAATAG AAAGCATCAA AGAGACCAGC 
CTCGGGGCTC AGGAGGAACA GACGCCTGCC GAGGCCAAGC CCCTGGACGA AGGAGAACTT 
GAAGCAGAGT GGTTGCAAGA TGTTGGTTTA TCAACCCTGA TCTCGGGTGA TGAGGAGGAA 
GATGGCAAAG CCTTGCTCTC TACTTTGACT CGAACCCAGG CAGCTGCAGT GAAGAAGAGA 
TACAATACTT ACACACAAAC CTTGAGGAAA AAGAACAAGC AGTCTGTCAG GGATGTCAGA 
GACATCTTCG GAACCAGCGA ATCTCCTCCA GATGAGTTTT GCTGTGTCCC AGCTCCTCTT 
CTGGATGTGA GCCCGGATGA AGAAAGGATG CCAACGGTCC CTTGCAGAAA TGGTCAGCTG 
CCAGGTGATA CCTGTGACAG CCACGCTAGC GAGCTGGATG GCGCCCGGGA GGGGAAAGCG 
CTGCCAGGAA TCATCAAAGC CAGCGGCCCC TTGCCAGATG GTGCTTCTCT CAACAGTAGT 
ACCCTGTCAG ATGGGTCCCA GGATGAAGAA TGGAGTTTTA CTGTTCCCAG GAGTGGCTCC 
ATGTCCATCC TGGAGGCCAT TCCAGACGTG CCAGTTCATT CCAACGTCGC GGCAGACCCT 
GGACGGTCGG CGCGGAACGC TGCAAGTGAT GATGAATATC TGGAAAAAAA CATTCCGGTA 
GAAGCTGAAG AGCTGTCCTT TGAAGTATCA TATTCAGAAA AGCTAACACA GGCTCCAAAA 
AGGAACAGAT TTAAGAAGTT AGATTTTAAG AAAGAAGACT ATGTTTTACC TAAATTTGTT 
GTTCAGAAAA CCAGATTTGG CCTAACCAAA GCAGAAGATC TGTCTGCGGA AGACATGAAG 
AAAATCCGCC ATCTCTCTCT GATTGAATTG ACGGCCTTTT TTGATGCCTT TCGAATTCAG 
CTGAAAAGAA ACAAAACAGA GAAAGTCAAA GGACGAGACA GTGGGATTTT TGGAGTTCCC 
CTCACAGTCC TGCTAGAAAA TGACCGAAGG AAAGACTCTG GGGTCAAAGT TCCCCTTGTG 
TTACAAAAGT TCTTTGAGAA AGTTGAGGAA TCGGGTCTGG AATCTGAAGG GATCTTTCGA 
CTTTCAGGAT GTACTGCCAA AGTCAAGCAA TACCGTGAAG AACTGGATGC CAAGTTTAAT 
GCTGACAAGT TTAAATGGGA GAAAATGTGC CATAGAGAAG CTGCAGTGAT GCTGAAAGCC 
TTTTTCAGAG AACTGCCCAC CTCTCTCTTT CCCGTGGAAT ATATACCTGC CTTCATCACT 
CTAATGGAAA GAGGGCCTCA CATCCGAGTA CAGTTTCAAG CCTTACACCT CATGGTCATG 
GCACTGCCTG ATGCCAACAG GGACACAGCC CAGGCCCTCA TGACATTCTT CAATAAAGTC 
ATTGCCAATG AATCAAAAAA TCGCATGAGT ATATGGAACA TTTCTACTGT CATGGCACCC 
AATCTTTTCT TCAGTAGAAG CAAACATTCT GACTGTGAAG AATTACTCTT AGCAAACACT 
GCTGCCCACA TCATCCGTCT GATGCTCAAG TACCAGGAGA TGTTGTGGAC GGTTCCGTCT 
TTCTTAATCT CTCAAGTCAG AAGAATGAAT GAAGCCACCA TGTTGTTAAA GAAGCCGCTC 
CCAAGTGTCA AAAAGCTGCT AAGGAGGAAG ACCATAGAAC GAGGGGTTAC AAGCCCCAAG 
ACATCAAAGG TACTGCAAAA ATCACCCTCT ACAAGAAGAA TGTCCGACGT GCCAGAGGGC 
GTCATAAGGG TCCACGCGCC CCTTCTCTCC AAGGTGTCCA TGGCCATTCA ACTCAACAGT 
CAAACCAAAG TCAAAGACAT CCTGGCAAAA TTTCAATGTG AGAACAGTCA TGGTTCATCG 
GAATGCATTA AGATCCAGAA CCAAAGGTTA TATGAAATTG GAGGTAATAT AGGACAGCAC 
TGCTTGGATC CGGATGCTTA TATACTGGAT GTTTATCATG TGAATCCCCA GGCAGAATGG 
GTGATTAAAC CCCAACAGAG TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP28 ( XM_010818947.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817249.1 Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X2, mRNA.

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CloneID	OBa51833
Clone ID Related Accession (Same CDS sequence)	XM_010825551.2 , XM_010818947.2
Accession Version	XM_010818947.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCA ATGAGTCCCT TCAGTCCCCG GCGTTCAGCC GCTCCAACTC TCAAGCCTCC 
GTAGACAGCG CCTCCATGGA GAATTTCTGG CGGGAAATAG AAAGCATCAA AGAGACCAGC 
CTCGGGGCTC AGGAGGAACA GACGCCTGCC GAGGCCAAGC CCCTGGACGA AGGAGAACTT 
GAAGCAGAGT GGTTGCAAGA TGTTGGTTTA TCAACCCTGA TCTCGGGTGA TGAGGAGGAA 
GATGGCAAAG CCTTGCTCTC TACTTTGACT CGAACCCAGG CAGCTGCAGT GAAGAAGAGA 
TACAATACTT ACACACAAAC CTTGAGGAAA AAGAACAAGC AGTCTGTCAG GGATGTCAGA 
GACATCTTCG GAACCAGCGA ATCTCCTCCA GATGAGTTTT GCTGTGTCCC AGCTCCTCTT 
CTGGATGTGA GCCCGGATGA AGAAAGGATG CCAACGGTCC CTTGCAGAAA TGGTCAGCTG 
CCAGGTGATA CCTGTGACAG CCACGCTAGC GAGCTGGATG GCGCCCGGGA GGGGAAAGCG 
CTGCCAGGAA TCATCAAAGC CAGCGGCCCC TTGCCAGATG GTGCTTCTCT CAACAGTAGT 
ACCCTGTCAG ATGGGTCCCA GGATGAAGAA TGGAGTTTTA CTGTTCCCAG GAGTGGCTCC 
ATGTCCATCC TGGAGGCCAT TCCAGACGTG CCAGTTCATT CCAACGTCGC GGCAGACCCT 
GGACGGTCGG CGCGGAACGC TGCAAGTGAT GATGAATATC TGGAAAAAAA CATTCCGGTA 
GAAGCTGAAG AGCTGTCCTT TGAAGTATCA TATTCAGAAA AGCTAACACA GGCTCCAAAA 
AGGAACAGAT TTAAGAAGTT AGATTTTAAG AAAGAAGACT ATGTTTTACC TAAATTTGTT 
GTTCAGAAAA CCAGATTTGG CCTAACCAAA GCAGAAGATC TGTCTGCGGA AGACATGAAG 
AAAATCCGCC ATCTCTCTCT GATTGAATTG ACGGCCTTTT TTGATGCCTT TCGAATTCAG 
CTGAAAAGAA ACAAAACAGA GAAAGTCAAA GGACGAGACA GTGGGATTTT TGGAGTTCCC 
CTCACAGTCC TGCTAGAAAA TGACCGAAGG AAAGACTCTG GGGTCAAAGT TCCCCTTGTG 
TTACAAAAGT TCTTTGAGAA AGTTGAGGAA TCGGGTCTGG AATCTGAAGG GATCTTTCGA 
CTTTCAGGAT GTACTGCCAA AGTCAAGCAA TACCGTGAAG AACTGGATGC CAAGTTTAAT 
GCTGACAAGT TTAAATGGGA GAAAATGTGC CATAGAGAAG CTGCAGTGAT GCTGAAAGCC 
TTTTTCAGAG AACTGCCCAC CTCTCTCTTT CCCGTGGAAT ATATACCTGC CTTCATCACT 
CTAATGGAAA GAGGGCCTCA CATCCGAGTA CAGTTTCAAG CCTTACACCT CATGGTCATG 
GCACTGCCTG ATGCCAACAG GGACACAGCC CAGGCCCTCA TGACATTCTT CAATAAAGTC 
ATTGCCAATG AATCAAAAAA TCGCATGAGT ATATGGAACA TTTCTACTGT CATGGCACCC 
AATCTTTTCT TCAGTAGAAG CAAACATTCT GACTGTGAAG AATTACTCTT AGCAAACACT 
GCTGCCCACA TCATCCGTCT GATGCTCAAG TACCAGGAGA TGTTGTGGAC GGTTCCGTCT 
TTCTTAATCT CTCAAGTCAG AAGAATGAAT GAAGCCACCA TGTTGTTAAA GAAGCCGCTC 
CCAAGTGTCA AAAAGCTGCT AAGGAGGAAG ACCATAGAAC GAGGGGTTAC AAGCCCCAAG 
ACATCAAAGG TACTGCAAAA ATCACCCTCT ACAAGAAGAA TGTCCGACGT GCCAGAGGGC 
GTCATAAGGG TCCACGCGCC CCTTCTCTCC AAGGTGTCCA TGGCCATTCA ACTCAACAGT 
CAAACCAAAG TCAAAGACAT CCTGGCAAAA TTTCAATGTG AGAACAGTCA TGGTTCATCG 
GAATGCATTA AGATCCAGAA CCAAAGGTTA TATGAAATTG GAGGTAATAT AGGACAGCAC 
TGCTTGGATC CGGATGCTTA TATACTGGAT GTTTATCATG TGAATCCCCA GGCAGAATGG 
GTGATTAAAC CCCAACAGAG TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817249.1
CDS	1147..3273
Translation	MLSNESLQSPAFSRSNSQASVDSASMENFWREIESIKETSLGAQEEQTPAEAKPLDEGELEAEWLQDVGLSTLISGDEEEDGKALLSTLTRTQAAAVKKRYNTYTQTLRKKNKQSVRDVRDIFGTSESPPDEFCCVPAPLLDVSPDEERMPTVPCRNGQLPGDTCDSHASELDGAREGKALPGIIKASGPLPDGASLNSSTLSDGSQDEEWSFTVPRSGSMSILEAIPDVPVHSNVAADPGRSARNAASDDEYLEKNIPVEAEELSFEVSYSEKLTQAPKRNRFKKLDFKKEDYVLPKFVVQKTRFGLTKAEDLSAEDMKKIRHLSLIELTAFFDAFRIQLKRNKTEKVKGRDSGIFGVPLTVLLENDRRKDSGVKVPLVLQKFFEKVEESGLESEGIFRLSGCTAKVKQYREELDAKFNADKFKWEKMCHREAAVMLKAFFRELPTSLFPVEYIPAFITLMERGPHIRVQFQALHLMVMALPDANRDTAQALMTFFNKVIANESKNRMSIWNISTVMAPNLFFSRSKHSDCEELLLANTAAHIIRLMLKYQEMLWTVPSFLISQVRRMNEATMLLKKPLPSVKKLLRRKTIERGVTSPKTSKVLQKSPSTRRMSDVPEGVIRVHAPLLSKVSMAIQLNSQTKVKDILAKFQCENSHGSSECIKIQNQRLYEIGGNIGQHCLDPDAYILDVYHVNPQAEWVIKPQQSV

Target ORF information:

RefSeq Version	XM_010818947.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 28 (ARHGAP28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818947.2

ORF Insert Sequence:

ATGCTCTCCA ATGAGTCCCT TCAGTCCCCG GCGTTCAGCC GCTCCAACTC TCAAGCCTCC 
GTAGACAGCG CCTCCATGGA GAATTTCTGG CGGGAAATAG AAAGCATCAA AGAGACCAGC 
CTCGGGGCTC AGGAGGAACA GACGCCTGCC GAGGCCAAGC CCCTGGACGA AGGAGAACTT 
GAAGCAGAGT GGTTGCAAGA TGTTGGTTTA TCAACCCTGA TCTCGGGTGA TGAGGAGGAA 
GATGGCAAAG CCTTGCTCTC TACTTTGACT CGAACCCAGG CAGCTGCAGT GAAGAAGAGA 
TACAATACTT ACACACAAAC CTTGAGGAAA AAGAACAAGC AGTCTGTCAG GGATGTCAGA 
GACATCTTCG GAACCAGCGA ATCTCCTCCA GATGAGTTTT GCTGTGTCCC AGCTCCTCTT 
CTGGATGTGA GCCCGGATGA AGAAAGGATG CCAACGGTCC CTTGCAGAAA TGGTCAGCTG 
CCAGGTGATA CCTGTGACAG CCACGCTAGC GAGCTGGATG GCGCCCGGGA GGGGAAAGCG 
CTGCCAGGAA TCATCAAAGC CAGCGGCCCC TTGCCAGATG GTGCTTCTCT CAACAGTAGT 
ACCCTGTCAG ATGGGTCCCA GGATGAAGAA TGGAGTTTTA CTGTTCCCAG GAGTGGCTCC 
ATGTCCATCC TGGAGGCCAT TCCAGACGTG CCAGTTCATT CCAACGTCGC GGCAGACCCT 
GGACGGTCGG CGCGGAACGC TGCAAGTGAT GATGAATATC TGGAAAAAAA CATTCCGGTA 
GAAGCTGAAG AGCTGTCCTT TGAAGTATCA TATTCAGAAA AGCTAACACA GGCTCCAAAA 
AGGAACAGAT TTAAGAAGTT AGATTTTAAG AAAGAAGACT ATGTTTTACC TAAATTTGTT 
GTTCAGAAAA CCAGATTTGG CCTAACCAAA GCAGAAGATC TGTCTGCGGA AGACATGAAG 
AAAATCCGCC ATCTCTCTCT GATTGAATTG ACGGCCTTTT TTGATGCCTT TCGAATTCAG 
CTGAAAAGAA ACAAAACAGA GAAAGTCAAA GGACGAGACA GTGGGATTTT TGGAGTTCCC 
CTCACAGTCC TGCTAGAAAA TGACCGAAGG AAAGACTCTG GGGTCAAAGT TCCCCTTGTG 
TTACAAAAGT TCTTTGAGAA AGTTGAGGAA TCGGGTCTGG AATCTGAAGG GATCTTTCGA 
CTTTCAGGAT GTACTGCCAA AGTCAAGCAA TACCGTGAAG AACTGGATGC CAAGTTTAAT 
GCTGACAAGT TTAAATGGGA GAAAATGTGC CATAGAGAAG CTGCAGTGAT GCTGAAAGCC 
TTTTTCAGAG AACTGCCCAC CTCTCTCTTT CCCGTGGAAT ATATACCTGC CTTCATCACT 
CTAATGGAAA GAGGGCCTCA CATCCGAGTA CAGTTTCAAG CCTTACACCT CATGGTCATG 
GCACTGCCTG ATGCCAACAG GGACACAGCC CAGGCCCTCA TGACATTCTT CAATAAAGTC 
ATTGCCAATG AATCAAAAAA TCGCATGAGT ATATGGAACA TTTCTACTGT CATGGCACCC 
AATCTTTTCT TCAGTAGAAG CAAACATTCT GACTGTGAAG AATTACTCTT AGCAAACACT 
GCTGCCCACA TCATCCGTCT GATGCTCAAG TACCAGGAGA TGTTGTGGAC GGTTCCGTCT 
TTCTTAATCT CTCAAGTCAG AAGAATGAAT GAAGCCACCA TGTTGTTAAA GAAGCCGCTC 
CCAAGTGTCA AAAAGCTGCT AAGGAGGAAG ACCATAGAAC GAGGGGTTAC AAGCCCCAAG 
ACATCAAAGG TACTGCAAAA ATCACCCTCT ACAAGAAGAA TGTCCGACGT GCCAGAGGGC 
GTCATAAGGG TCCACGCGCC CCTTCTCTCC AAGGTGTCCA TGGCCATTCA ACTCAACAGT 
CAAACCAAAG TCAAAGACAT CCTGGCAAAA TTTCAATGTG AGAACAGTCA TGGTTCATCG 
GAATGCATTA AGATCCAGAA CCAAAGGTTA TATGAAATTG GAGGTAATAT AGGACAGCAC 
TGCTTGGATC CGGATGCTTA TATACTGGAT GTTTATCATG TGAATCCCCA GGCAGAATGG 
GTGATTAAAC CCCAACAGAG TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ARHGAP28 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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