DAGLA cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DAGLA
Entrez Gene ID	523665
Full Name	diacylglycerol lipase alpha
General protein information	Preferred Names diacylglycerol lipase alpha Names sn1-specific diacylglycerol lipase alpha
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001192583.3	NP_001179512.1	sn1-specific diacylglycerol lipase alpha
XM_005226931.4	XP_005226988.1	sn1-specific diacylglycerol lipase alpha isoform X1
XM_005226930.3	XP_005226987.1	sn1-specific diacylglycerol lipase alpha isoform X1
XM_005226930.4	XP_005226987.1	sn1-specific diacylglycerol lipase alpha isoform X1
XM_005226931.3	XP_005226988.1	sn1-specific diacylglycerol lipase alpha isoform X1
NM_001192583.1	NP_001179512.1	sn1-specific diacylglycerol lipase alpha
NM_001192583.2	NP_001179512.1	sn1-specific diacylglycerol lipase alpha

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	dagla	XP_697873.5
Homo sapiens (human)	DAGLA	NP_006124.1
Canis lupus familiaris (dog)	DAGLA	XP_005631813.1
Mus musculus (house mouse)	Dagla	NP_932782.2
Gallus gallus (chicken)	DAGLA	XP_423696.3
Xenopus tropicalis (tropical clawed frog)	dagla	XP_004913466.1
Rattus norvegicus (Norway rat)	Dagla	NP_001005886.1
Pan troglodytes (chimpanzee)	DAGLA	XP_508479.4
Bos taurus (cattle)	DAGLA	NP_001179512.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DAGLA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAGLA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa231998	NM_001192583.3 Latest version!	Bos taurus diacylglycerol lipase alpha (DAGLA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa34664	NM_001192583.2 Latest version!	Bos taurus diacylglycerol lipase alpha (DAGLA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa37613	NM_001192583.1 Latest version!	Bos taurus diacylglycerol lipase, alpha (DAGLA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa231998	XM_005226931.4 Latest version!	Bos taurus diacylglycerol lipase alpha (DAGLA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa231998	XM_005226930.4 Latest version!	Bos taurus diacylglycerol lipase alpha (DAGLA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa34664	XM_005226931.3 Latest version!	Bos taurus diacylglycerol lipase, alpha (DAGLA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa34664	XM_005226930.3 Latest version!	Bos taurus diacylglycerol lipase, alpha (DAGLA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAGLA ( NM_001192583.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179512.1 Bos taurus diacylglycerol lipase alpha (DAGLA), mRNA.

Price & Availability↑

CloneID	OBa231998
Clone ID Related Accession (Same CDS sequence)	NM_001192583.3 , XM_005226931.4 , XM_005226930.4
Accession Version	NM_001192583.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527523200000
Organism	Bos taurus(cattle)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000029.1. On May 30, 2018 this sequence version replaced NM_001192583.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR6764281.8000.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT TTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179512.1
CDS	1..3126
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPDEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRESMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPVPAGLALELPAADHRNSSIRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANEEPEEGGGGAAPRSGELALQEGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAVDKVRTSTPAGRGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	NM_001192583.3
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol lipase alpha (DAGLA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192583.3

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT TTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( NM_001192583.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179512.1 Bos taurus diacylglycerol lipase alpha (DAGLA), mRNA.

Price & Availability↑

CloneID	OBa34664
Clone ID Related Accession (Same CDS sequence)	XM_005226930.3 , XM_005226931.3 , NM_001192583.2
Accession Version	NM_001192583.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1508601600000
Organism	Bos taurus(cattle)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02063470.1. On Jun 30, 2017 this sequence version replaced NM_001192583.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179512.1
CDS	1..3126
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPDEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRESMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPVPAGLALELPAADHRNSSIRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANEEPEEGGGGAAPRSGELALQEGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAVDKVRTSTPAGRGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	NM_001192583.2
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol lipase alpha (DAGLA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192583.2

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( NM_001192583.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179512.1 Bos taurus diacylglycerol lipase, alpha (DAGLA), mRNA.

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CloneID	OBa37613
Clone ID Related Accession (Same CDS sequence)	NM_001192583.1
Accession Version	NM_001192583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1420214400000
Organism	Bos taurus(Cattle)
Product	sn1-specific diacylglycerol lipase alpha
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03045766.1. On Jul 17, 2010 this sequence version replaced XM_601967.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01093727, SAMN01093728 [ECO:0000348] ##Evidence-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGATGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179512.1
CDS	1..3126
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPDEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRESMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPVPAGLALELPAADHRNSSIRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANEEPEEGGGGAAPRSGELALQEGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAVDKVRTSTPAGRGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	NM_001192583.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus diacylglycerol lipase, alpha (DAGLA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192583.1

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGATGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_005226931.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226988.1 Bos taurus diacylglycerol lipase alpha (DAGLA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa231998
Clone ID Related Accession (Same CDS sequence)	NM_001192583.3 , XM_005226931.4 , XM_005226930.4
Accession Version	XM_005226931.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226931.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT TTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226988.1
CDS	179..3304
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPDEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRESMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPVPAGLALELPAADHRNSSIRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANEEPEEGGGGAAPRSGELALQEGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAVDKVRTSTPAGRGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	XM_005226931.4
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol lipase alpha (DAGLA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226931.4

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT TTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_005226930.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226987.1 Bos taurus diacylglycerol lipase alpha (DAGLA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa231998
Clone ID Related Accession (Same CDS sequence)	NM_001192583.3 , XM_005226931.4 , XM_005226930.4
Accession Version	XM_005226930.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226930.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT TTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226987.1
CDS	2752..5877
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPDEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRESMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPVPAGLALELPAADHRNSSIRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANEEPEEGGGGAAPRSGELALQEGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAVDKVRTSTPAGRGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	XM_005226930.4
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol lipase alpha (DAGLA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226930.4

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT TTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_005226930.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226987.1 Bos taurus diacylglycerol lipase, alpha (DAGLA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa34664
Clone ID Related Accession (Same CDS sequence)	XM_005226930.3 , XM_005226931.3 , NM_001192583.2
Accession Version	XM_005226930.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104646.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226930.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226987.1
CDS	5032..8157
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPDEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRESMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPVPAGLALELPAADHRNSSIRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANEEPEEGGGGAAPRSGELALQEGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAVDKVRTSTPAGRGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	XM_005226930.3
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol lipase, alpha (DAGLA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226930.3

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_005226931.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226988.1 Bos taurus diacylglycerol lipase, alpha (DAGLA), transcript variant X1, mRNA.

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CloneID	OBa34664
Clone ID Related Accession (Same CDS sequence)	XM_005226930.3 , XM_005226931.3 , NM_001192583.2
Accession Version	XM_005226931.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104646.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226988.1
CDS	186..3311
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPDEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRESMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPVPAGLALELPAADHRNSSIRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANEEPEEGGGGAAPRSGELALQEGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAVDKVRTSTPAGRGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	XM_005226931.3
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol lipase, alpha (DAGLA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226931.3

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCCTG 
CCAGCCATCT TCCTCTTCCT GCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTACAACCC GGACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATTTTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGCG GCATCCTCTA CACAGAGCCC CGCGAATCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTAAT CGAGTTCATC TATGCCATCG TGGGCATCGT CTGGCTCACC 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAACG TCACGCTCGG GATGGTCGTC 
TGCAACTGGG TGGTCATCCT GAGTGTGTGC ATCACGGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TCGTCAAGCT GAGGGCCACC AAGAGGCGGC AGCGCAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA AGAGGGTCAG GCCACCAGCT GGTCACGCCG GCTCAAAGTG 
TTCCTCTGCT GCACTCGGAC GAAGGACTCC CAGTCAGATG CCTACTCGGA AATCGCCTAC 
CTCTTTGCCG AGTTTTTCCG AGACCTGGAC ATTGTGCCGT CGGACATCAT CGCCGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAATGCCG TGCTGGACGA GGCCAACAAC 
GACATCCTGG CCTTCCTGTC TGGCATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT CCGCTATAAG GAGGTCTGCT ATTACATGCT CTTTGCCCTG 
GCTGCCTATG GCTGGCCCAT GTACCTGATG CGGAAACCCG CCTGCGGCCT CTGCCAGCTG 
GCCCGGTCCT GCTCGTGCTG CTTGTGCCCC GCCCGGCCCC GCTTCGCCCC CGGAGTCACC 
ATCGAGGAAG ACAACTGCTG CGGCTGCAAC GCCATCGCCA TTCGACGCCA CTTCCTGGAC 
GAGAACATGA CCGCGGTGGA CATCGTCTAC ACGTCCTGCC ACGACGCGGT CTATGAAACC 
CCCTTCTACG TGGCGGTGGA CCACGACAAG AAGAAGGTGG TGATCAGTAT CCGGGGAACC 
CTGTCCCCCA AGGACGCCCT GACAGACCTG ACCGGTGATG CCGAGCGCCT CCCCGTGGAG 
GGACACCACG GCACCTGGCT GGGACACAAG GGGATGGTCC TCTCGGCCGA GTATATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTCG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTCGGC CACTCCCTGG GCGCGGGCAC CGCCGCCATC 
CTCTCCTTCC TCCTGAGACC CCAGTACCCA ACCCTCAAGT GCTTTGCCTA CTCCCCCCCT 
GGGGGCCTGC TGAGCGAGGA CGCCATGGAG TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTTGTTCC CAGGATTGGC CTCTCCCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GAATCATTGT GGGGGCCACC 
AAATGCATCC CCAAGTCAGA GCTGCCCGAG GAGGTGGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CTCATCCCAG CGACCTGACC ATCGCCCTGT CAGCCAGCAC TCCCCTCTAC 
CCCCCTGGCC GCATCATCCA CGTGGTCCAC AATCACCCAG CGGAGCAGTG CTGCTGCTGC 
GAGCAGGAGG AGCCCACGTA CTTCGCCATC TGGGGCGACA ACAAGGCCTT CAACGAGGTG 
ATCATCTCGC CGGCCATGCT GCACGAGCAC CTCCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACTGCCCTGC TGTCCGCTGC AAAGGTCATG 
GTGAGCCCCA CAGAGGTGGA CCTAACCCCC GAGCTCATCT TCCAGCAGCA GCCGCTGCCC 
ACGGGGCCAC CCGTGCCTGC TGGCCTGGCC CTGGAGCTGC CCGCTGCAGA CCACCGAAAC 
AGCAGCATCA GGAGCAAGTC CCAATCTGAG ATGAGCCTGG AGGGCTTCTC CGAGGGGCGG 
CTGCTCTCCC CAGTGGCCGC GGCCTCAGCT GCCCGCCAGG ACCCGGTAGA GCTGCTGCTG 
CTGTCTACCC AGGAGCGGCT GGCAGCTGAG CTGCAGGCGC GGCGGGCGCC GCTGGCCACC 
ATGGAGAGTC TGTCGGACAC GGAGTCTCTG TACAGCTTCG ACTCGCGCCG TTCCTCCGGC 
TTCCGCAGCA TCCGCGGCTC GCCTAGCCTC CACGCCGTGC TGGAGCGCGA CGAGGGCCAC 
CTCTTCTACA TCGACCCGGC CATCCCCGAG GAGAACCCAT CCCTGAGTTC GCGCACCGAG 
CTGCTGGCAG CCGACAGCCT GTCCAAGCAC TCGCAGGACA CGCAGCCCCT GGAGGCCGCC 
CTGGGCAGTG GGGGCGTCAC CCCCGAGCGG CCCCCCAGCG CGGCGGCCAA TGAGGAGCCG 
GAGGAAGGGG GCGGAGGGGC AGCCCCCCGC AGCGGAGAGC TGGCGCTGCA GGAGGGGCGC 
CTGGGGGACT CACCCAGCCC GCAGGTGCTG GAGTTCGCCG AGTTCATCGA CAGCCTCTTC 
AACTTGGACA GCAAGAGCAG TTCCTTCCAG GACCTCTACT GCATGGTGGT GCCGGAGAGC 
CCCACCAGCG ACTACGCGGA GGGCCCCAAG TCCCCCAGCC AGCAGGAGAT CCTGCTCCGA 
GCCCAGTTCG AGCCCAATCT GGTGCCCAAG CCCCCACGGC TCTTCGCTGG CTCAGCCGAC 
CCCTCCTCGG GCATCTCGCT GTCACCCTCC TTCCCACTCA GCTCCTCGGG CGAGCTCATG 
GACCTGACTC CCACGGGCCT CAGCAGCCAG GAGTGCCTGG CAGTGGACAA GGTCCGGACT 
TCTACCCCGG CTGGCCGTGG GGCCAGCCCC ACCAAGCAGG ATGACCTGGT CATCTCGGCA 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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DAGLA cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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