MELK cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MELK
Entrez Gene ID	520088
Full Name	maternal embryonic leucine zipper kinase
General protein information	Preferred Names maternal embryonic leucine zipper kinase Names maternal embryonic leucine zipper kinase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001111260.1	NP_001104730.1	maternal embryonic leucine zipper kinase
XM_010807979.2	XP_010806281.1	maternal embryonic leucine zipper kinase isoform X1
XM_024995724.1	XP_024851492.1	maternal embryonic leucine zipper kinase isoform X1
XM_024995723.1	XP_024851491.1	maternal embryonic leucine zipper kinase isoform X1
XM_024995725.1	XP_024851493.1	maternal embryonic leucine zipper kinase isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MELK	XP_538730.2
Gallus gallus (chicken)	MELK	NP_001026680.1
Xenopus tropicalis (tropical clawed frog)	melk	NP_001016390.1
Rattus norvegicus (Norway rat)	Melk	NP_001102132.1
Pan troglodytes (chimpanzee)	MELK	XP_001169038.1
Mus musculus (house mouse)	Melk	NP_034920.2
Danio rerio (zebrafish)	melk	NP_996771.2
Arabidopsis thaliana (thale cress)	CIPK7	NP_188940.1
Homo sapiens (human)	MELK	NP_055606.1
Macaca mulatta (Rhesus monkey)	MELK	XP_001115076.2
Bos taurus (cattle)	MELK	NP_001104730.1
Caenorhabditis elegans (roundworm)	pig-1	NP_001023420.2
Arabidopsis thaliana (thale cress)	CIPK4	NP_193194.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MELK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MELK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36099	NM_001111260.1 Latest version!	Bos taurus maternal embryonic leucine zipper kinase (MELK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa237087	XM_024995725.1 Latest version!	Bos taurus maternal embryonic leucine zipper kinase (MELK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa50183	XM_024995723.1 Latest version!	Bos taurus maternal embryonic leucine zipper kinase (MELK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa50183	XM_010807979.2 Latest version!	Bos taurus maternal embryonic leucine zipper kinase (MELK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa50183	XM_024995724.1 Latest version!	Bos taurus maternal embryonic leucine zipper kinase (MELK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MELK ( NM_001111260.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001104730.1 Bos taurus maternal embryonic leucine zipper kinase (MELK), mRNA.

Price & Availability↑

CloneID	OBa36099
Clone ID Related Accession (Same CDS sequence)	NM_001111260.1
Accession Version	NM_001111260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1531411200000
Organism	Bos taurus(cattle)
Product	maternal embryonic leucine zipper kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF446902.1. On Dec 12, 2007 this sequence version replaced XM_598320.3. ##Evidence-Data-START## Transcript exon combination :: EF446902.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145426, SAMN03145433 [ECO:0000348] ##Evidence-Data-END##

ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGG TCAAAACAGA AATTGATGCC 
TTGAAGAACC TGAGACATCA ACATATATGT CAACTCTACC ATGTGATAGA GACAGCCAAC 
AAAATATTCA TGGTTCTCGA GTATTGCCCT GGAGGAGAGC TGTTTGACTA CATAATTTCC 
CACGATCGCC TGTCAGAGGA GGAGACCCGC ATTGTCTTCC GTCAGATAGT GTCTGCAGTT 
GCTTATGTGC ACAGCCAGGG CTACGCTCAC AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGATTATC ACCTGCAGAC CTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATT 
CAAGGCAAAT CATATCTGGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GACGATAATG TCATGGCTTT GTACAAAAAG 
ATTATGAAAG GGCAATATGA CGTTCCAAAG TGGCTGTCTC CCAGTAGCGT TCTACTTCTT 
CAGCAAATGC TACAGGTGGA CCCAAAGAAA CGGATTGCTA TGAGAAATCT CTTGAACCAC 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AAAGCAATGC TCCTCTTATT 
CACCTTGATG AAGATTGTGT AACGGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACT 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CAGCTACTTA CCTTCTGCTC 
CTAGCCAAGA AGGCTCGGGG GAGACCGGTT CGTTTAAGGC TTCTTTCTTC CTATGGACAG 
GCCAGCTCAA CGCCAGTCAC AAACATAAAG TCGAAGAATC TGAGTCTAGA AGATATGATC 
ACAAGTGATG AAAATTATGT GACTGGATTA ATGAGCTATG AATGGTGTGA AGACATTTCA 
TCAAGAGGTG TGGCTACTCC ACAAACACCA CAGGTTGCCA AGCATTGGAC AGAATCAAAT 
GACCTGGAAT CTACATCATT AACTCCAGTA TTATGCAGGG CATCTGCCAA TAAATTAAAG 
AACAAAGAAA ATGTATATAC TCCTAAGTCT GCTGTAAAGA ATGAAGAGTG CTTTGTATTT 
CCTGAGCCAA AGACTCCAGT TAATAAGAAC CAGTATAAAA GAGAAATACT CACTACACCA 
AATCATTACA GTACACCCTC AAAAGCTGGA AACCAGTTCC TGAAAGAAAC CCCAATTAAA 
ATGCCGGTAA ATTCAACAGG AACAGAGAAG TTACTAACAG GAGTCATTAG CCCTGAGAGG 
CGGTGTCGCT CAGTAGAACT CAATCTCAAC CAAGTACATG TAGAGGAAAC TCCAAAAAGA 
AAAGGAACCA AAGTGTTTGG GAGCCTTGAG AAGGGGTTGG ATAAGGTTAT CACAGTGCTT 
ACCAGGAGCA AAAGGAAAGG CTCTGTCAAA GATGGGCCAA GAAGACTAAA GCCTCACTAT 
AATGTGACTA CAACCAGATT AGTGAATCCA GATCAACTCT TGAATGAAAT AATGTCTGTT 
CTTCCAAAGA AGCACGTTGA CTTTGTACAG AAGGGCTATA CACTGAAGTG TCAGACACAG 
TCAGATTTTG GCAAAGTGAC AATGCAGTTT GAACTAGAAG TGTGCCAGCT TCAAAAACCC 
GATGTGGTGG GTATCAGGAG GCAGCGGCTT AAGGGTGATG CCTGGGTTTA CAAGAGATTA 
GTGGAAGACA TCCTATCTAG CTGCAAGGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104730.1
CDS	148..2100
Translation	MKDYDELLKYYELYETIGTGGFAKVKLACHILTGEMVAIKIMDKNALGSDLPRVKTEIDALKNLRHQHICQLYHVIETANKIFMVLEYCPGGELFDYIISHDRLSEEETRIVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALYKKIMKGQYDVPKWLSPSSVLLLQQMLQVDPKKRIAMRNLLNHPWIMHDYNCPVEWQSNAPLIHLDEDCVTELSVHHRNNRQTMEDLISLWQYDHLTATYLLLLAKKARGRPVRLRLLSSYGQASSTPVTNIKSKNLSLEDMITSDENYVTGLMSYEWCEDISSRGVATPQTPQVAKHWTESNDLESTSLTPVLCRASANKLKNKENVYTPKSAVKNEECFVFPEPKTPVNKNQYKREILTTPNHYSTPSKAGNQFLKETPIKMPVNSTGTEKLLTGVISPERRCRSVELNLNQVHVEETPKRKGTKVFGSLEKGLDKVITVLTRSKRKGSVKDGPRRLKPHYNVTTTRLVNPDQLLNEIMSVLPKKHVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQKPDVVGIRRQRLKGDAWVYKRLVEDILSSCKV

Target ORF information:

RefSeq Version	NM_001111260.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maternal embryonic leucine zipper kinase (MELK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001111260.1

ORF Insert Sequence:

ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGG TCAAAACAGA AATTGATGCC 
TTGAAGAACC TGAGACATCA ACATATATGT CAACTCTACC ATGTGATAGA GACAGCCAAC 
AAAATATTCA TGGTTCTCGA GTATTGCCCT GGAGGAGAGC TGTTTGACTA CATAATTTCC 
CACGATCGCC TGTCAGAGGA GGAGACCCGC ATTGTCTTCC GTCAGATAGT GTCTGCAGTT 
GCTTATGTGC ACAGCCAGGG CTACGCTCAC AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGATTATC ACCTGCAGAC CTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATT 
CAAGGCAAAT CATATCTGGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GACGATAATG TCATGGCTTT GTACAAAAAG 
ATTATGAAAG GGCAATATGA CGTTCCAAAG TGGCTGTCTC CCAGTAGCGT TCTACTTCTT 
CAGCAAATGC TACAGGTGGA CCCAAAGAAA CGGATTGCTA TGAGAAATCT CTTGAACCAC 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AAAGCAATGC TCCTCTTATT 
CACCTTGATG AAGATTGTGT AACGGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACT 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CAGCTACTTA CCTTCTGCTC 
CTAGCCAAGA AGGCTCGGGG GAGACCGGTT CGTTTAAGGC TTCTTTCTTC CTATGGACAG 
GCCAGCTCAA CGCCAGTCAC AAACATAAAG TCGAAGAATC TGAGTCTAGA AGATATGATC 
ACAAGTGATG AAAATTATGT GACTGGATTA ATGAGCTATG AATGGTGTGA AGACATTTCA 
TCAAGAGGTG TGGCTACTCC ACAAACACCA CAGGTTGCCA AGCATTGGAC AGAATCAAAT 
GACCTGGAAT CTACATCATT AACTCCAGTA TTATGCAGGG CATCTGCCAA TAAATTAAAG 
AACAAAGAAA ATGTATATAC TCCTAAGTCT GCTGTAAAGA ATGAAGAGTG CTTTGTATTT 
CCTGAGCCAA AGACTCCAGT TAATAAGAAC CAGTATAAAA GAGAAATACT CACTACACCA 
AATCATTACA GTACACCCTC AAAAGCTGGA AACCAGTTCC TGAAAGAAAC CCCAATTAAA 
ATGCCGGTAA ATTCAACAGG AACAGAGAAG TTACTAACAG GAGTCATTAG CCCTGAGAGG 
CGGTGTCGCT CAGTAGAACT CAATCTCAAC CAAGTACATG TAGAGGAAAC TCCAAAAAGA 
AAAGGAACCA AAGTGTTTGG GAGCCTTGAG AAGGGGTTGG ATAAGGTTAT CACAGTGCTT 
ACCAGGAGCA AAAGGAAAGG CTCTGTCAAA GATGGGCCAA GAAGACTAAA GCCTCACTAT 
AATGTGACTA CAACCAGATT AGTGAATCCA GATCAACTCT TGAATGAAAT AATGTCTGTT 
CTTCCAAAGA AGCACGTTGA CTTTGTACAG AAGGGCTATA CACTGAAGTG TCAGACACAG 
TCAGATTTTG GCAAAGTGAC AATGCAGTTT GAACTAGAAG TGTGCCAGCT TCAAAAACCC 
GATGTGGTGG GTATCAGGAG GCAGCGGCTT AAGGGTGATG CCTGGGTTTA CAAGAGATTA 
GTGGAAGACA TCCTATCTAG CTGCAAGGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MELK ( XM_024995725.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851493.1 Bos taurus maternal embryonic leucine zipper kinase (MELK), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa237087
Clone ID Related Accession (Same CDS sequence)	XM_024995725.1
Accession Version	XM_024995725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	maternal embryonic leucine zipper kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGG TCAAAACAGA AATTGATGCC 
TTGAAGAACC TGAGACATCA ACATATATGT CAACTCTACC ATGTGATAGA GACAGCCAAC 
AAAATATTCA TGGTTCTCGA GTATTGCCCT GGAGGAGAGC TGTTTGACTA CATAATTTCC 
CACGATCGCC TGTCAGAGGA GGAGACCCGC ATTGTCTTCC GTCAGATAGT GTCTGCAGTT 
GCTTATGTGC ACAGCCAGGG CTACGCTCAC AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGATTATC ACCTGCAGAC CTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATT 
CAAGGCAAAT CATATCTGGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GACGATAATG TCATGGCTTT GTACAAAAAG 
ATTATGAAAG GGCAATATGA CGTTCCAAAG TGGCTGTCTC CCAGTAGCGT TCTACTTCTT 
CAGCAAATGC TACAGCTTAT TCACCTTGAT GAAGATTGTG TAACGGAACT TTCTGTACAT 
CACAGAAATA ACAGGCAAAC TATGGAGGAT TTAATTTCAC TGTGGCAGTA TGATCACCTC 
ACAGCTACTT ACCTTCTGCT CCTAGCCAAG AAGGCTCGGG GGAGACCGGT TCGTTTAAGG 
CTTCTTTCTT CCTATGGACA GGCCAGCTCA ACGCCAGTCA CAAACATAAA GTCGAAGAAT 
CTGAGTCTAG AAGATATGAT CACAAGTGAT GAAAATTATG TGACTGGATT AATGAGCTAT 
GAATGGTGTG AAGACATTTC ATCAAGAGGT GTGGCTACTC CACAAACACC ACAGTTTGCC 
AAGCATTGGA CAGAATCAAA TGACCTGGAA TCTACATCAT TAACTCCAGT ATTATGCAGG 
GCATCTGCCA ATAAATTAAA GAACAAAGAA AATGTATATA CTCCTAAGTC TGCTGTAAAG 
AATGAAGAGT GCTTTGTATT TCCTGAGCCA AAGACTCCAG TTAATAAGAA CCAGTATAAA 
AGAGAAATAC TCACTACACC AAATCATTAC AGTACACCCT CAAAAGCTGG AAACCAGTTC 
CTGAAAGAAA CCCCAATTAA AATGCCGGTA AATTCAACAG GAACAGAGAA GTTACTAACA 
GGAGTCATTA GCCCTGAGAG GCGGTGTCGC TCAGTAGAAC TCAATCTCAA CCAAGTACAT 
GTAGAGGAAA CTCCAAAAAG AAAAGGAACC AAAGTGTTTG GGAGCCTTGA GAAGGGGTTG 
GATAAGGTTA TCACAGTGCT TACCAGGAGC AAAAGGAAAG GCTCTGTCAA AGATGGGCCA 
AGAAGACTAA AGCCTCACTA TAATGTGACT ACAACCAGAT TAGTGAATCC AGATCAACTC 
TTGAATGAAA TAATGTCTGT TCTTCCAAAG AAGCACGTTG ACTTTGTACA GAAGGGCTAT 
ACACTGAAGT GTCAGACACA GTCAGATTTT GGCAAAGTGA CAATGCAGTT TGAACTAGAA 
GTGTGCCAGC TTCAAAAACC CGATGTGGTG GGTATCAGGA GGCAGCGGCT TAAGGGTGAT 
GCCTGGGTTT ACAAGAGATT AGTGGAAGAC ATCCTATCTA GCTGCAAGGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851493.1
CDS	206..2059
Translation	MKDYDELLKYYELYETIGTGGFAKVKLACHILTGEMVAIKIMDKNALGSDLPRVKTEIDALKNLRHQHICQLYHVIETANKIFMVLEYCPGGELFDYIISHDRLSEEETRIVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALYKKIMKGQYDVPKWLSPSSVLLLQQMLQLIHLDEDCVTELSVHHRNNRQTMEDLISLWQYDHLTATYLLLLAKKARGRPVRLRLLSSYGQASSTPVTNIKSKNLSLEDMITSDENYVTGLMSYEWCEDISSRGVATPQTPQFAKHWTESNDLESTSLTPVLCRASANKLKNKENVYTPKSAVKNEECFVFPEPKTPVNKNQYKREILTTPNHYSTPSKAGNQFLKETPIKMPVNSTGTEKLLTGVISPERRCRSVELNLNQVHVEETPKRKGTKVFGSLEKGLDKVITVLTRSKRKGSVKDGPRRLKPHYNVTTTRLVNPDQLLNEIMSVLPKKHVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQKPDVVGIRRQRLKGDAWVYKRLVEDILSSCKV

Target ORF information:

RefSeq Version	XM_024995725.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maternal embryonic leucine zipper kinase (MELK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995725.1

ORF Insert Sequence:

ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGG TCAAAACAGA AATTGATGCC 
TTGAAGAACC TGAGACATCA ACATATATGT CAACTCTACC ATGTGATAGA GACAGCCAAC 
AAAATATTCA TGGTTCTCGA GTATTGCCCT GGAGGAGAGC TGTTTGACTA CATAATTTCC 
CACGATCGCC TGTCAGAGGA GGAGACCCGC ATTGTCTTCC GTCAGATAGT GTCTGCAGTT 
GCTTATGTGC ACAGCCAGGG CTACGCTCAC AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGATTATC ACCTGCAGAC CTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATT 
CAAGGCAAAT CATATCTGGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GACGATAATG TCATGGCTTT GTACAAAAAG 
ATTATGAAAG GGCAATATGA CGTTCCAAAG TGGCTGTCTC CCAGTAGCGT TCTACTTCTT 
CAGCAAATGC TACAGCTTAT TCACCTTGAT GAAGATTGTG TAACGGAACT TTCTGTACAT 
CACAGAAATA ACAGGCAAAC TATGGAGGAT TTAATTTCAC TGTGGCAGTA TGATCACCTC 
ACAGCTACTT ACCTTCTGCT CCTAGCCAAG AAGGCTCGGG GGAGACCGGT TCGTTTAAGG 
CTTCTTTCTT CCTATGGACA GGCCAGCTCA ACGCCAGTCA CAAACATAAA GTCGAAGAAT 
CTGAGTCTAG AAGATATGAT CACAAGTGAT GAAAATTATG TGACTGGATT AATGAGCTAT 
GAATGGTGTG AAGACATTTC ATCAAGAGGT GTGGCTACTC CACAAACACC ACAGTTTGCC 
AAGCATTGGA CAGAATCAAA TGACCTGGAA TCTACATCAT TAACTCCAGT ATTATGCAGG 
GCATCTGCCA ATAAATTAAA GAACAAAGAA AATGTATATA CTCCTAAGTC TGCTGTAAAG 
AATGAAGAGT GCTTTGTATT TCCTGAGCCA AAGACTCCAG TTAATAAGAA CCAGTATAAA 
AGAGAAATAC TCACTACACC AAATCATTAC AGTACACCCT CAAAAGCTGG AAACCAGTTC 
CTGAAAGAAA CCCCAATTAA AATGCCGGTA AATTCAACAG GAACAGAGAA GTTACTAACA 
GGAGTCATTA GCCCTGAGAG GCGGTGTCGC TCAGTAGAAC TCAATCTCAA CCAAGTACAT 
GTAGAGGAAA CTCCAAAAAG AAAAGGAACC AAAGTGTTTG GGAGCCTTGA GAAGGGGTTG 
GATAAGGTTA TCACAGTGCT TACCAGGAGC AAAAGGAAAG GCTCTGTCAA AGATGGGCCA 
AGAAGACTAA AGCCTCACTA TAATGTGACT ACAACCAGAT TAGTGAATCC AGATCAACTC 
TTGAATGAAA TAATGTCTGT TCTTCCAAAG AAGCACGTTG ACTTTGTACA GAAGGGCTAT 
ACACTGAAGT GTCAGACACA GTCAGATTTT GGCAAAGTGA CAATGCAGTT TGAACTAGAA 
GTGTGCCAGC TTCAAAAACC CGATGTGGTG GGTATCAGGA GGCAGCGGCT TAAGGGTGAT 
GCCTGGGTTT ACAAGAGATT AGTGGAAGAC ATCCTATCTA GCTGCAAGGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MELK ( XM_010807979.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010806281.1 Bos taurus maternal embryonic leucine zipper kinase (MELK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa50183
Clone ID Related Accession (Same CDS sequence)	XM_010807979.2 , XM_024995724.1 , XM_024995723.1
Accession Version	XM_010807979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	maternal embryonic leucine zipper kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104104.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807979.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGG TCAAAACAGA AATTGATGCC 
TTGAAGAACC TGAGACATCA ACATATATGT CAACTCTACC ATGTGATAGA GACAGCCAAC 
AAAATATTCA TGGTTCTCGA GTATTGCCCT GGAGGAGAGC TGTTTGACTA CATAATTTCC 
CACGATCGCC TGTCAGAGGA GGAGACCCGC ATTGTCTTCC GTCAGATAGT GTCTGCAGTT 
GCTTATGTGC ACAGCCAGGG CTACGCTCAC AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGATTATC ACCTGCAGAC CTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATT 
CAAGGCAAAT CATATCTGGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GACGATAATG TCATGGCTTT GTACAAAAAG 
ATTATGAAAG GGCAATATGA CGTTCCAAAG TGGCTGTCTC CCAGTAGCGT TCTACTTCTT 
CAGCAAATGC TACAGGTGGA CCCAAAGAAA CGGATTGCTA TGAGAAATCT CTTGAACCAC 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AAAGCAATGC TCCTCTTATT 
CACCTTGATG AAGATTGTGT AACGGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACT 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CAGCTACTTA CCTTCTGCTC 
CTAGCCAAGA AGGCTCGGGG GAGACCGGTT CGTTTAAGGC TTCTTTCTTC CTATGGACAG 
GCCAGCTCAA CGCCAGTCAC AAACATAAAG TCGAAGAATC TGAGTCTAGA AGATATGATC 
ACAAGTGATG AAAATTATGT GACTGGATTA ATGAGCTATG AATGGTGTGA AGACATTTCA 
TCAAGAGGTG TGGCTACTCC ACAAACACCA CAGTTTGCCA AGCATTGGAC AGAATCAAAT 
GACCTGGAAT CTACATCATT AACTCCAGTA TTATGCAGGG CATCTGCCAA TAAATTAAAG 
AACAAAGAAA ATGTATATAC TCCTAAGTCT GCTGTAAAGA ATGAAGAGTG CTTTGTATTT 
CCTGAGCCAA AGACTCCAGT TAATAAGAAC CAGTATAAAA GAGAAATACT CACTACACCA 
AATCATTACA GTACACCCTC AAAAGCTGGA AACCAGTTCC TGAAAGAAAC CCCAATTAAA 
ATGCCGGTAA ATTCAACAGG AACAGAGAAG TTACTAACAG GAGTCATTAG CCCTGAGAGG 
CGGTGTCGCT CAGTAGAACT CAATCTCAAC CAAGTACATG TAGAGGAAAC TCCAAAAAGA 
AAAGGAACCA AAGTGTTTGG GAGCCTTGAG AAGGGGTTGG ATAAGGTTAT CACAGTGCTT 
ACCAGGAGCA AAAGGAAAGG CTCTGTCAAA GATGGGCCAA GAAGACTAAA GCCTCACTAT 
AATGTGACTA CAACCAGATT AGTGAATCCA GATCAACTCT TGAATGAAAT AATGTCTGTT 
CTTCCAAAGA AGCACGTTGA CTTTGTACAG AAGGGCTATA CACTGAAGTG TCAGACACAG 
TCAGATTTTG GCAAAGTGAC AATGCAGTTT GAACTAGAAG TGTGCCAGCT TCAAAAACCC 
GATGTGGTGG GTATCAGGAG GCAGCGGCTT AAGGGTGATG CCTGGGTTTA CAAGAGATTA 
GTGGAAGACA TCCTATCTAG CTGCAAGGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010806281.1
CDS	419..2371
Translation	MKDYDELLKYYELYETIGTGGFAKVKLACHILTGEMVAIKIMDKNALGSDLPRVKTEIDALKNLRHQHICQLYHVIETANKIFMVLEYCPGGELFDYIISHDRLSEEETRIVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALYKKIMKGQYDVPKWLSPSSVLLLQQMLQVDPKKRIAMRNLLNHPWIMHDYNCPVEWQSNAPLIHLDEDCVTELSVHHRNNRQTMEDLISLWQYDHLTATYLLLLAKKARGRPVRLRLLSSYGQASSTPVTNIKSKNLSLEDMITSDENYVTGLMSYEWCEDISSRGVATPQTPQFAKHWTESNDLESTSLTPVLCRASANKLKNKENVYTPKSAVKNEECFVFPEPKTPVNKNQYKREILTTPNHYSTPSKAGNQFLKETPIKMPVNSTGTEKLLTGVISPERRCRSVELNLNQVHVEETPKRKGTKVFGSLEKGLDKVITVLTRSKRKGSVKDGPRRLKPHYNVTTTRLVNPDQLLNEIMSVLPKKHVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQKPDVVGIRRQRLKGDAWVYKRLVEDILSSCKV

Target ORF information:

RefSeq Version	XM_010807979.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maternal embryonic leucine zipper kinase (MELK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807979.2

ORF Insert Sequence:

ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGG TCAAAACAGA AATTGATGCC 
TTGAAGAACC TGAGACATCA ACATATATGT CAACTCTACC ATGTGATAGA GACAGCCAAC 
AAAATATTCA TGGTTCTCGA GTATTGCCCT GGAGGAGAGC TGTTTGACTA CATAATTTCC 
CACGATCGCC TGTCAGAGGA GGAGACCCGC ATTGTCTTCC GTCAGATAGT GTCTGCAGTT 
GCTTATGTGC ACAGCCAGGG CTACGCTCAC AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGATTATC ACCTGCAGAC CTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATT 
CAAGGCAAAT CATATCTGGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GACGATAATG TCATGGCTTT GTACAAAAAG 
ATTATGAAAG GGCAATATGA CGTTCCAAAG TGGCTGTCTC CCAGTAGCGT TCTACTTCTT 
CAGCAAATGC TACAGGTGGA CCCAAAGAAA CGGATTGCTA TGAGAAATCT CTTGAACCAC 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AAAGCAATGC TCCTCTTATT 
CACCTTGATG AAGATTGTGT AACGGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACT 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CAGCTACTTA CCTTCTGCTC 
CTAGCCAAGA AGGCTCGGGG GAGACCGGTT CGTTTAAGGC TTCTTTCTTC CTATGGACAG 
GCCAGCTCAA CGCCAGTCAC AAACATAAAG TCGAAGAATC TGAGTCTAGA AGATATGATC 
ACAAGTGATG AAAATTATGT GACTGGATTA ATGAGCTATG AATGGTGTGA AGACATTTCA 
TCAAGAGGTG TGGCTACTCC ACAAACACCA CAGTTTGCCA AGCATTGGAC AGAATCAAAT 
GACCTGGAAT CTACATCATT AACTCCAGTA TTATGCAGGG CATCTGCCAA TAAATTAAAG 
AACAAAGAAA ATGTATATAC TCCTAAGTCT GCTGTAAAGA ATGAAGAGTG CTTTGTATTT 
CCTGAGCCAA AGACTCCAGT TAATAAGAAC CAGTATAAAA GAGAAATACT CACTACACCA 
AATCATTACA GTACACCCTC AAAAGCTGGA AACCAGTTCC TGAAAGAAAC CCCAATTAAA 
ATGCCGGTAA ATTCAACAGG AACAGAGAAG TTACTAACAG GAGTCATTAG CCCTGAGAGG 
CGGTGTCGCT CAGTAGAACT CAATCTCAAC CAAGTACATG TAGAGGAAAC TCCAAAAAGA 
AAAGGAACCA AAGTGTTTGG GAGCCTTGAG AAGGGGTTGG ATAAGGTTAT CACAGTGCTT 
ACCAGGAGCA AAAGGAAAGG CTCTGTCAAA GATGGGCCAA GAAGACTAAA GCCTCACTAT 
AATGTGACTA CAACCAGATT AGTGAATCCA GATCAACTCT TGAATGAAAT AATGTCTGTT 
CTTCCAAAGA AGCACGTTGA CTTTGTACAG AAGGGCTATA CACTGAAGTG TCAGACACAG 
TCAGATTTTG GCAAAGTGAC AATGCAGTTT GAACTAGAAG TGTGCCAGCT TCAAAAACCC 
GATGTGGTGG GTATCAGGAG GCAGCGGCTT AAGGGTGATG CCTGGGTTTA CAAGAGATTA 
GTGGAAGACA TCCTATCTAG CTGCAAGGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MELK ( XM_024995724.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851492.1 Bos taurus maternal embryonic leucine zipper kinase (MELK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa50183
Clone ID Related Accession (Same CDS sequence)	XM_010807979.2 , XM_024995724.1 , XM_024995723.1
Accession Version	XM_024995724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	maternal embryonic leucine zipper kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGG TCAAAACAGA AATTGATGCC 
TTGAAGAACC TGAGACATCA ACATATATGT CAACTCTACC ATGTGATAGA GACAGCCAAC 
AAAATATTCA TGGTTCTCGA GTATTGCCCT GGAGGAGAGC TGTTTGACTA CATAATTTCC 
CACGATCGCC TGTCAGAGGA GGAGACCCGC ATTGTCTTCC GTCAGATAGT GTCTGCAGTT 
GCTTATGTGC ACAGCCAGGG CTACGCTCAC AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGATTATC ACCTGCAGAC CTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATT 
CAAGGCAAAT CATATCTGGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GACGATAATG TCATGGCTTT GTACAAAAAG 
ATTATGAAAG GGCAATATGA CGTTCCAAAG TGGCTGTCTC CCAGTAGCGT TCTACTTCTT 
CAGCAAATGC TACAGGTGGA CCCAAAGAAA CGGATTGCTA TGAGAAATCT CTTGAACCAC 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AAAGCAATGC TCCTCTTATT 
CACCTTGATG AAGATTGTGT AACGGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACT 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CAGCTACTTA CCTTCTGCTC 
CTAGCCAAGA AGGCTCGGGG GAGACCGGTT CGTTTAAGGC TTCTTTCTTC CTATGGACAG 
GCCAGCTCAA CGCCAGTCAC AAACATAAAG TCGAAGAATC TGAGTCTAGA AGATATGATC 
ACAAGTGATG AAAATTATGT GACTGGATTA ATGAGCTATG AATGGTGTGA AGACATTTCA 
TCAAGAGGTG TGGCTACTCC ACAAACACCA CAGTTTGCCA AGCATTGGAC AGAATCAAAT 
GACCTGGAAT CTACATCATT AACTCCAGTA TTATGCAGGG CATCTGCCAA TAAATTAAAG 
AACAAAGAAA ATGTATATAC TCCTAAGTCT GCTGTAAAGA ATGAAGAGTG CTTTGTATTT 
CCTGAGCCAA AGACTCCAGT TAATAAGAAC CAGTATAAAA GAGAAATACT CACTACACCA 
AATCATTACA GTACACCCTC AAAAGCTGGA AACCAGTTCC TGAAAGAAAC CCCAATTAAA 
ATGCCGGTAA ATTCAACAGG AACAGAGAAG TTACTAACAG GAGTCATTAG CCCTGAGAGG 
CGGTGTCGCT CAGTAGAACT CAATCTCAAC CAAGTACATG TAGAGGAAAC TCCAAAAAGA 
AAAGGAACCA AAGTGTTTGG GAGCCTTGAG AAGGGGTTGG ATAAGGTTAT CACAGTGCTT 
ACCAGGAGCA AAAGGAAAGG CTCTGTCAAA GATGGGCCAA GAAGACTAAA GCCTCACTAT 
AATGTGACTA CAACCAGATT AGTGAATCCA GATCAACTCT TGAATGAAAT AATGTCTGTT 
CTTCCAAAGA AGCACGTTGA CTTTGTACAG AAGGGCTATA CACTGAAGTG TCAGACACAG 
TCAGATTTTG GCAAAGTGAC AATGCAGTTT GAACTAGAAG TGTGCCAGCT TCAAAAACCC 
GATGTGGTGG GTATCAGGAG GCAGCGGCTT AAGGGTGATG CCTGGGTTTA CAAGAGATTA 
GTGGAAGACA TCCTATCTAG CTGCAAGGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851492.1
CDS	248..2200
Translation	MKDYDELLKYYELYETIGTGGFAKVKLACHILTGEMVAIKIMDKNALGSDLPRVKTEIDALKNLRHQHICQLYHVIETANKIFMVLEYCPGGELFDYIISHDRLSEEETRIVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALYKKIMKGQYDVPKWLSPSSVLLLQQMLQVDPKKRIAMRNLLNHPWIMHDYNCPVEWQSNAPLIHLDEDCVTELSVHHRNNRQTMEDLISLWQYDHLTATYLLLLAKKARGRPVRLRLLSSYGQASSTPVTNIKSKNLSLEDMITSDENYVTGLMSYEWCEDISSRGVATPQTPQFAKHWTESNDLESTSLTPVLCRASANKLKNKENVYTPKSAVKNEECFVFPEPKTPVNKNQYKREILTTPNHYSTPSKAGNQFLKETPIKMPVNSTGTEKLLTGVISPERRCRSVELNLNQVHVEETPKRKGTKVFGSLEKGLDKVITVLTRSKRKGSVKDGPRRLKPHYNVTTTRLVNPDQLLNEIMSVLPKKHVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQKPDVVGIRRQRLKGDAWVYKRLVEDILSSCKV

Target ORF information:

RefSeq Version	XM_024995724.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maternal embryonic leucine zipper kinase (MELK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995724.1

ORF Insert Sequence:

ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGG TCAAAACAGA AATTGATGCC 
TTGAAGAACC TGAGACATCA ACATATATGT CAACTCTACC ATGTGATAGA GACAGCCAAC 
AAAATATTCA TGGTTCTCGA GTATTGCCCT GGAGGAGAGC TGTTTGACTA CATAATTTCC 
CACGATCGCC TGTCAGAGGA GGAGACCCGC ATTGTCTTCC GTCAGATAGT GTCTGCAGTT 
GCTTATGTGC ACAGCCAGGG CTACGCTCAC AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGATTATC ACCTGCAGAC CTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATT 
CAAGGCAAAT CATATCTGGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GACGATAATG TCATGGCTTT GTACAAAAAG 
ATTATGAAAG GGCAATATGA CGTTCCAAAG TGGCTGTCTC CCAGTAGCGT TCTACTTCTT 
CAGCAAATGC TACAGGTGGA CCCAAAGAAA CGGATTGCTA TGAGAAATCT CTTGAACCAC 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AAAGCAATGC TCCTCTTATT 
CACCTTGATG AAGATTGTGT AACGGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACT 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CAGCTACTTA CCTTCTGCTC 
CTAGCCAAGA AGGCTCGGGG GAGACCGGTT CGTTTAAGGC TTCTTTCTTC CTATGGACAG 
GCCAGCTCAA CGCCAGTCAC AAACATAAAG TCGAAGAATC TGAGTCTAGA AGATATGATC 
ACAAGTGATG AAAATTATGT GACTGGATTA ATGAGCTATG AATGGTGTGA AGACATTTCA 
TCAAGAGGTG TGGCTACTCC ACAAACACCA CAGTTTGCCA AGCATTGGAC AGAATCAAAT 
GACCTGGAAT CTACATCATT AACTCCAGTA TTATGCAGGG CATCTGCCAA TAAATTAAAG 
AACAAAGAAA ATGTATATAC TCCTAAGTCT GCTGTAAAGA ATGAAGAGTG CTTTGTATTT 
CCTGAGCCAA AGACTCCAGT TAATAAGAAC CAGTATAAAA GAGAAATACT CACTACACCA 
AATCATTACA GTACACCCTC AAAAGCTGGA AACCAGTTCC TGAAAGAAAC CCCAATTAAA 
ATGCCGGTAA ATTCAACAGG AACAGAGAAG TTACTAACAG GAGTCATTAG CCCTGAGAGG 
CGGTGTCGCT CAGTAGAACT CAATCTCAAC CAAGTACATG TAGAGGAAAC TCCAAAAAGA 
AAAGGAACCA AAGTGTTTGG GAGCCTTGAG AAGGGGTTGG ATAAGGTTAT CACAGTGCTT 
ACCAGGAGCA AAAGGAAAGG CTCTGTCAAA GATGGGCCAA GAAGACTAAA GCCTCACTAT 
AATGTGACTA CAACCAGATT AGTGAATCCA GATCAACTCT TGAATGAAAT AATGTCTGTT 
CTTCCAAAGA AGCACGTTGA CTTTGTACAG AAGGGCTATA CACTGAAGTG TCAGACACAG 
TCAGATTTTG GCAAAGTGAC AATGCAGTTT GAACTAGAAG TGTGCCAGCT TCAAAAACCC 
GATGTGGTGG GTATCAGGAG GCAGCGGCTT AAGGGTGATG CCTGGGTTTA CAAGAGATTA 
GTGGAAGACA TCCTATCTAG CTGCAAGGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MELK ( XM_024995723.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851491.1 Bos taurus maternal embryonic leucine zipper kinase (MELK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa50183
Clone ID Related Accession (Same CDS sequence)	XM_010807979.2 , XM_024995724.1 , XM_024995723.1
Accession Version	XM_024995723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	maternal embryonic leucine zipper kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGG TCAAAACAGA AATTGATGCC 
TTGAAGAACC TGAGACATCA ACATATATGT CAACTCTACC ATGTGATAGA GACAGCCAAC 
AAAATATTCA TGGTTCTCGA GTATTGCCCT GGAGGAGAGC TGTTTGACTA CATAATTTCC 
CACGATCGCC TGTCAGAGGA GGAGACCCGC ATTGTCTTCC GTCAGATAGT GTCTGCAGTT 
GCTTATGTGC ACAGCCAGGG CTACGCTCAC AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGATTATC ACCTGCAGAC CTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATT 
CAAGGCAAAT CATATCTGGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GACGATAATG TCATGGCTTT GTACAAAAAG 
ATTATGAAAG GGCAATATGA CGTTCCAAAG TGGCTGTCTC CCAGTAGCGT TCTACTTCTT 
CAGCAAATGC TACAGGTGGA CCCAAAGAAA CGGATTGCTA TGAGAAATCT CTTGAACCAC 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AAAGCAATGC TCCTCTTATT 
CACCTTGATG AAGATTGTGT AACGGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACT 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CAGCTACTTA CCTTCTGCTC 
CTAGCCAAGA AGGCTCGGGG GAGACCGGTT CGTTTAAGGC TTCTTTCTTC CTATGGACAG 
GCCAGCTCAA CGCCAGTCAC AAACATAAAG TCGAAGAATC TGAGTCTAGA AGATATGATC 
ACAAGTGATG AAAATTATGT GACTGGATTA ATGAGCTATG AATGGTGTGA AGACATTTCA 
TCAAGAGGTG TGGCTACTCC ACAAACACCA CAGTTTGCCA AGCATTGGAC AGAATCAAAT 
GACCTGGAAT CTACATCATT AACTCCAGTA TTATGCAGGG CATCTGCCAA TAAATTAAAG 
AACAAAGAAA ATGTATATAC TCCTAAGTCT GCTGTAAAGA ATGAAGAGTG CTTTGTATTT 
CCTGAGCCAA AGACTCCAGT TAATAAGAAC CAGTATAAAA GAGAAATACT CACTACACCA 
AATCATTACA GTACACCCTC AAAAGCTGGA AACCAGTTCC TGAAAGAAAC CCCAATTAAA 
ATGCCGGTAA ATTCAACAGG AACAGAGAAG TTACTAACAG GAGTCATTAG CCCTGAGAGG 
CGGTGTCGCT CAGTAGAACT CAATCTCAAC CAAGTACATG TAGAGGAAAC TCCAAAAAGA 
AAAGGAACCA AAGTGTTTGG GAGCCTTGAG AAGGGGTTGG ATAAGGTTAT CACAGTGCTT 
ACCAGGAGCA AAAGGAAAGG CTCTGTCAAA GATGGGCCAA GAAGACTAAA GCCTCACTAT 
AATGTGACTA CAACCAGATT AGTGAATCCA GATCAACTCT TGAATGAAAT AATGTCTGTT 
CTTCCAAAGA AGCACGTTGA CTTTGTACAG AAGGGCTATA CACTGAAGTG TCAGACACAG 
TCAGATTTTG GCAAAGTGAC AATGCAGTTT GAACTAGAAG TGTGCCAGCT TCAAAAACCC 
GATGTGGTGG GTATCAGGAG GCAGCGGCTT AAGGGTGATG CCTGGGTTTA CAAGAGATTA 
GTGGAAGACA TCCTATCTAG CTGCAAGGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851491.1
CDS	172..2124
Translation	MKDYDELLKYYELYETIGTGGFAKVKLACHILTGEMVAIKIMDKNALGSDLPRVKTEIDALKNLRHQHICQLYHVIETANKIFMVLEYCPGGELFDYIISHDRLSEEETRIVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALYKKIMKGQYDVPKWLSPSSVLLLQQMLQVDPKKRIAMRNLLNHPWIMHDYNCPVEWQSNAPLIHLDEDCVTELSVHHRNNRQTMEDLISLWQYDHLTATYLLLLAKKARGRPVRLRLLSSYGQASSTPVTNIKSKNLSLEDMITSDENYVTGLMSYEWCEDISSRGVATPQTPQFAKHWTESNDLESTSLTPVLCRASANKLKNKENVYTPKSAVKNEECFVFPEPKTPVNKNQYKREILTTPNHYSTPSKAGNQFLKETPIKMPVNSTGTEKLLTGVISPERRCRSVELNLNQVHVEETPKRKGTKVFGSLEKGLDKVITVLTRSKRKGSVKDGPRRLKPHYNVTTTRLVNPDQLLNEIMSVLPKKHVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQKPDVVGIRRQRLKGDAWVYKRLVEDILSSCKV

Target ORF information:

RefSeq Version	XM_024995723.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maternal embryonic leucine zipper kinase (MELK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995723.1

ORF Insert Sequence:

ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAT ATCCTCACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGG TCAAAACAGA AATTGATGCC 
TTGAAGAACC TGAGACATCA ACATATATGT CAACTCTACC ATGTGATAGA GACAGCCAAC 
AAAATATTCA TGGTTCTCGA GTATTGCCCT GGAGGAGAGC TGTTTGACTA CATAATTTCC 
CACGATCGCC TGTCAGAGGA GGAGACCCGC ATTGTCTTCC GTCAGATAGT GTCTGCAGTT 
GCTTATGTGC ACAGCCAGGG CTACGCTCAC AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGATTATC ACCTGCAGAC CTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATT 
CAAGGCAAAT CATATCTGGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GACGATAATG TCATGGCTTT GTACAAAAAG 
ATTATGAAAG GGCAATATGA CGTTCCAAAG TGGCTGTCTC CCAGTAGCGT TCTACTTCTT 
CAGCAAATGC TACAGGTGGA CCCAAAGAAA CGGATTGCTA TGAGAAATCT CTTGAACCAC 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AAAGCAATGC TCCTCTTATT 
CACCTTGATG AAGATTGTGT AACGGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACT 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CAGCTACTTA CCTTCTGCTC 
CTAGCCAAGA AGGCTCGGGG GAGACCGGTT CGTTTAAGGC TTCTTTCTTC CTATGGACAG 
GCCAGCTCAA CGCCAGTCAC AAACATAAAG TCGAAGAATC TGAGTCTAGA AGATATGATC 
ACAAGTGATG AAAATTATGT GACTGGATTA ATGAGCTATG AATGGTGTGA AGACATTTCA 
TCAAGAGGTG TGGCTACTCC ACAAACACCA CAGTTTGCCA AGCATTGGAC AGAATCAAAT 
GACCTGGAAT CTACATCATT AACTCCAGTA TTATGCAGGG CATCTGCCAA TAAATTAAAG 
AACAAAGAAA ATGTATATAC TCCTAAGTCT GCTGTAAAGA ATGAAGAGTG CTTTGTATTT 
CCTGAGCCAA AGACTCCAGT TAATAAGAAC CAGTATAAAA GAGAAATACT CACTACACCA 
AATCATTACA GTACACCCTC AAAAGCTGGA AACCAGTTCC TGAAAGAAAC CCCAATTAAA 
ATGCCGGTAA ATTCAACAGG AACAGAGAAG TTACTAACAG GAGTCATTAG CCCTGAGAGG 
CGGTGTCGCT CAGTAGAACT CAATCTCAAC CAAGTACATG TAGAGGAAAC TCCAAAAAGA 
AAAGGAACCA AAGTGTTTGG GAGCCTTGAG AAGGGGTTGG ATAAGGTTAT CACAGTGCTT 
ACCAGGAGCA AAAGGAAAGG CTCTGTCAAA GATGGGCCAA GAAGACTAAA GCCTCACTAT 
AATGTGACTA CAACCAGATT AGTGAATCCA GATCAACTCT TGAATGAAAT AATGTCTGTT 
CTTCCAAAGA AGCACGTTGA CTTTGTACAG AAGGGCTATA CACTGAAGTG TCAGACACAG 
TCAGATTTTG GCAAAGTGAC AATGCAGTTT GAACTAGAAG TGTGCCAGCT TCAAAAACCC 
GATGTGGTGG GTATCAGGAG GCAGCGGCTT AAGGGTGATG CCTGGGTTTA CAAGAGATTA 
GTGGAAGACA TCCTATCTAG CTGCAAGGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MELK cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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