DESI2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DESI2
Entrez Gene ID	519650
Full Name	desumoylating isopeptidase 2
Synonyms	FAM152A,PPPDE1
General protein information	Preferred Names desumoylating isopeptidase 2 Names desumoylating isopeptidase 2 PPPDE peptidase domain containing 1 family with sequence similarity 152, member A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015475048.1	XP_015330534.1	desumoylating isopeptidase 2 isoform X3
XM_010813122.3	XP_010811424.1	deubiquitinase DESI2 isoform X1
XM_005216811.4	XP_005216868.1	deubiquitinase DESI2 isoform X2
XM_005216811.3	XP_005216868.1	desumoylating isopeptidase 2 isoform X2
NM_001100384.2	NP_001093854.1	deubiquitinase DESI2
XM_010813122.2	XP_010811424.1	desumoylating isopeptidase 2 isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012058	XP_320465.2
Homo sapiens (human)	DESI2	NP_057160.2
Drosophila melanogaster (fruit fly)	CG7222	NP_610613.1
Caenorhabditis elegans (roundworm)	F36D4.5	NP_741591.1
Oryza sativa (rice)	Os03g0100900	NP_001048648.1
Pan troglodytes (chimpanzee)	DESI2	XP_003949780.1
Macaca mulatta (Rhesus monkey)	PPPDE1	XP_001102905.2
Canis lupus familiaris (dog)	DESI2	XP_005622658.1
Bos taurus (cattle)	PPPDE1	NP_001093854.1
Mus musculus (house mouse)	Desi2	NP_077244.1
Rattus norvegicus (Norway rat)	Desi2	NP_001013895.1
Gallus gallus (chicken)	PPPDE1	NP_001008460.1
Danio rerio (zebrafish)	desi2	NP_001003532.1
Arabidopsis thaliana (thale cress)	AT4G17486	NP_567528.2
Arabidopsis thaliana (thale cress)	AT5G47310	NP_199542.1
Xenopus tropicalis (tropical clawed frog)	desi2	XP_002942241.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DESI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DESI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa38477	NM_001100384.2 Latest version!	Bos taurus desumoylating isopeptidase 2 (DESI2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OBa27077	XM_005216811.4 Latest version!	Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OBa50115	XM_010813122.3 Latest version!	Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OBa92901	XM_015475048.1 Latest version!	Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OBa27077	XM_005216811.3	Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OBa50115	XM_010813122.2	Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DESI2 ( NM_001100384.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001093854.1 Bos taurus desumoylating isopeptidase 2 (DESI2), mRNA.

Price & Availability↑

CloneID	OBa38477
Clone ID Related Accession (Same CDS sequence)	NM_001100384.2
Accession Version	NM_001100384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	deubiquitinase DESI2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC149676.1. On Aug 30, 2012 this sequence version replaced NM_001100384.1. ##Evidence-Data-START## Transcript exon combination :: BC149676.1, DT835552.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145464 [ECO:0000350] ##Evidence-Data-END##

ATGAACGAAT ACACCTCTTC CATCGGAATT GGAGTTTTTC ATTCAGGAAT TGAAGTATAT 
GGCAGAGAAT TTGCTTATGG TGGCCATCCT TATCCCTTTT CTGGAATATT TGAAATTTCC 
CCAGGAAATG CTTCTGAACT AGGAGAAACA TTTAAATTTA AAGAAGCTGT TGTTTTAGGG 
AGCACTGACT TCCTAGAAGA TGATATAGAA AAAATTGTGG AAGAATTGGG AAAAGAATAC 
AAAGGCAATG CCTATCATTT GATGCATAAA AATTGCAATC ACTTTTCTTC GGCTTTATCA 
GAGATTCTCT GTGGGAAAGA GATTCCTCGC TGGATCAATC GGCTTGCCTA CTTCAGCTCC 
TGTATACCTT TTCTACAGAG TTGCCTCCCA AAGGAGTGGC TCACGCCCGC AGCCCTGCAG 
TCCAGTGTCA GCCAGGAGCT CCAGGACGAA CTGGAGGAAG CAGAGGACGC TGCAGCCTCG 
GCTTCCATGG CCAGCACCAC CGCGGGCTCC AGACCTGGGC GCCACACCAA ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093854.1
CDS	70..606
Translation	MNEYTSSIGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNASELGETFKFKEAVVLGSTDFLEDDIEKIVEELGKEYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLAYFSSCIPFLQSCLPKEWLTPAALQSSVSQELQDELEEAEDAAASASMASTTAGSRPGRHTKL

Target ORF information:

RefSeq Version	NM_001100384.2
Organism	Bos taurus(cattle)
Definition	Bos taurus desumoylating isopeptidase 2 (DESI2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100384.2

ORF Insert Sequence:

ATGAACGAAT ACACCTCTTC CATCGGAATT GGAGTTTTTC ATTCAGGAAT TGAAGTATAT 
GGCAGAGAAT TTGCTTATGG TGGCCATCCT TATCCCTTTT CTGGAATATT TGAAATTTCC 
CCAGGAAATG CTTCTGAACT AGGAGAAACA TTTAAATTTA AAGAAGCTGT TGTTTTAGGG 
AGCACTGACT TCCTAGAAGA TGATATAGAA AAAATTGTGG AAGAATTGGG AAAAGAATAC 
AAAGGCAATG CCTATCATTT GATGCATAAA AATTGCAATC ACTTTTCTTC GGCTTTATCA 
GAGATTCTCT GTGGGAAAGA GATTCCTCGC TGGATCAATC GGCTTGCCTA CTTCAGCTCC 
TGTATACCTT TTCTACAGAG TTGCCTCCCA AAGGAGTGGC TCACGCCCGC AGCCCTGCAG 
TCCAGTGTCA GCCAGGAGCT CCAGGACGAA CTGGAGGAAG CAGAGGACGC TGCAGCCTCG 
GCTTCCATGG CCAGCACCAC CGCGGGCTCC AGACCTGGGC GCCACACCAA ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DESI2 ( XM_005216811.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216868.1 Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa27077
Clone ID Related Accession (Same CDS sequence)	XM_005216811.4 , XM_005216811.3
Accession Version	XM_005216811.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	deubiquitinase DESI2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216811.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCTA ACCAGTTAGT GGTGCTCAAC GTGTACGACA TGTACTGGAT GAACGAATAC 
ACCTCTTCCA TCGGAATTGG AGTTTTTCAT TCAGGAATTG AAGTATATGG CAGAGAATTT 
GCTTATGGTG GCCATCCTTA TCCCTTTTCT GGAATATTTG AAATTTCCCC AGGAAATGCT 
TCTGAACTAG GAGAAACATT TAAATTTAAA GAAGCTGTTG TTTTAGGGAG CACTGACTTC 
CTAGAAGATG ATATAGAAAA AATTGTGGAA GAATTGGGAA AAGAATACAA AGGCAATGCC 
TATCATTTGA TGCATAAAAA TTGCAATCAC TTTTCTTCGG CTTTATCAGA GATTCTCTGT 
GGGAAAGAGA TTCCTCGCTG GATCAATCGG CTTGCCTACT TCAGCTCCTG TATACCTTTT 
CTACAGAGTT GCCTCCCAAA GGAGTGGCTC ACGCCCGCAG CCCTGCAGTC CAGTGTCAGC 
CAGGAGCTCC AGGACGAACT GGAGGAAGCA GAGGACGCTG CAGCCTCGGC TTCCATGGCC 
AGCACCACCG CGGGCTCCAG ACCTGGGCGC CACACCAAAC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216868.1
CDS	240..824
Translation	MGANQLVVLNVYDMYWMNEYTSSIGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNASELGETFKFKEAVVLGSTDFLEDDIEKIVEELGKEYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLAYFSSCIPFLQSCLPKEWLTPAALQSSVSQELQDELEEAEDAAASASMASTTAGSRPGRHTKL

Target ORF information:

RefSeq Version	XM_005216811.4
Organism	Bos taurus(cattle)
Definition	Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216811.4

ORF Insert Sequence:

ATGGGGGCTA ACCAGTTAGT GGTGCTCAAC GTGTACGACA TGTACTGGAT GAACGAATAC 
ACCTCTTCCA TCGGAATTGG AGTTTTTCAT TCAGGAATTG AAGTATATGG CAGAGAATTT 
GCTTATGGTG GCCATCCTTA TCCCTTTTCT GGAATATTTG AAATTTCCCC AGGAAATGCT 
TCTGAACTAG GAGAAACATT TAAATTTAAA GAAGCTGTTG TTTTAGGGAG CACTGACTTC 
CTAGAAGATG ATATAGAAAA AATTGTGGAA GAATTGGGAA AAGAATACAA AGGCAATGCC 
TATCATTTGA TGCATAAAAA TTGCAATCAC TTTTCTTCGG CTTTATCAGA GATTCTCTGT 
GGGAAAGAGA TTCCTCGCTG GATCAATCGG CTTGCCTACT TCAGCTCCTG TATACCTTTT 
CTACAGAGTT GCCTCCCAAA GGAGTGGCTC ACGCCCGCAG CCCTGCAGTC CAGTGTCAGC 
CAGGAGCTCC AGGACGAACT GGAGGAAGCA GAGGACGCTG CAGCCTCGGC TTCCATGGCC 
AGCACCACCG CGGGCTCCAG ACCTGGGCGC CACACCAAAC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DESI2 ( XM_005216811.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216868.1 Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa27077
Clone ID Related Accession (Same CDS sequence)	XM_005216811.4 , XM_005216811.3
Accession Version	XM_005216811.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	desumoylating isopeptidase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104436.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216811.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCTA ACCAGTTAGT GGTGCTCAAC GTGTACGACA TGTACTGGAT GAACGAATAC 
ACCTCTTCCA TCGGAATTGG AGTTTTTCAT TCAGGAATTG AAGTATATGG CAGAGAATTT 
GCTTATGGTG GCCATCCTTA TCCCTTTTCT GGAATATTTG AAATTTCCCC AGGAAATGCT 
TCTGAACTAG GAGAAACATT TAAATTTAAA GAAGCTGTTG TTTTAGGGAG CACTGACTTC 
CTAGAAGATG ATATAGAAAA AATTGTGGAA GAATTGGGAA AAGAATACAA AGGCAATGCC 
TATCATTTGA TGCATAAAAA TTGCAATCAC TTTTCTTCGG CTTTATCAGA GATTCTCTGT 
GGGAAAGAGA TTCCTCGCTG GATCAATCGG CTTGCCTACT TCAGCTCCTG TATACCTTTT 
CTACAGAGTT GCCTCCCAAA GGAGTGGCTC ACGCCCGCAG CCCTGCAGTC CAGTGTCAGC 
CAGGAGCTCC AGGACGAACT GGAGGAAGCA GAGGACGCTG CAGCCTCGGC TTCCATGGCC 
AGCACCACCG CGGGCTCCAG ACCTGGGCGC CACACCAAAC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216868.1
CDS	231..815
Translation	MGANQLVVLNVYDMYWMNEYTSSIGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNASELGETFKFKEAVVLGSTDFLEDDIEKIVEELGKEYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLAYFSSCIPFLQSCLPKEWLTPAALQSSVSQELQDELEEAEDAAASASMASTTAGSRPGRHTKL

Target ORF information:

RefSeq Version	XM_005216811.3
Organism	Bos taurus(cattle)
Definition	Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216811.3

ORF Insert Sequence:

ATGGGGGCTA ACCAGTTAGT GGTGCTCAAC GTGTACGACA TGTACTGGAT GAACGAATAC 
ACCTCTTCCA TCGGAATTGG AGTTTTTCAT TCAGGAATTG AAGTATATGG CAGAGAATTT 
GCTTATGGTG GCCATCCTTA TCCCTTTTCT GGAATATTTG AAATTTCCCC AGGAAATGCT 
TCTGAACTAG GAGAAACATT TAAATTTAAA GAAGCTGTTG TTTTAGGGAG CACTGACTTC 
CTAGAAGATG ATATAGAAAA AATTGTGGAA GAATTGGGAA AAGAATACAA AGGCAATGCC 
TATCATTTGA TGCATAAAAA TTGCAATCAC TTTTCTTCGG CTTTATCAGA GATTCTCTGT 
GGGAAAGAGA TTCCTCGCTG GATCAATCGG CTTGCCTACT TCAGCTCCTG TATACCTTTT 
CTACAGAGTT GCCTCCCAAA GGAGTGGCTC ACGCCCGCAG CCCTGCAGTC CAGTGTCAGC 
CAGGAGCTCC AGGACGAACT GGAGGAAGCA GAGGACGCTG CAGCCTCGGC TTCCATGGCC 
AGCACCACCG CGGGCTCCAG ACCTGGGCGC CACACCAAAC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DESI2 ( XM_010813122.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811424.1 Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa50115
Clone ID Related Accession (Same CDS sequence)	XM_010813122.3 , XM_010813122.2
Accession Version	XM_010813122.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	deubiquitinase DESI2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813122.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCAGG AACTTCTCTC CTTTTTGTTG AGAAATAATG TAATAAAGTA CTGGATGAAC 
GAATACACCT CTTCCATCGG AATTGGAGTT TTTCATTCAG GAATTGAAGT ATATGGCAGA 
GAATTTGCTT ATGGTGGCCA TCCTTATCCC TTTTCTGGAA TATTTGAAAT TTCCCCAGGA 
AATGCTTCTG AACTAGGAGA AACATTTAAA TTTAAAGAAG CTGTTGTTTT AGGGAGCACT 
GACTTCCTAG AAGATGATAT AGAAAAAATT GTGGAAGAAT TGGGAAAAGA ATACAAAGGC 
AATGCCTATC ATTTGATGCA TAAAAATTGC AATCACTTTT CTTCGGCTTT ATCAGAGATT 
CTCTGTGGGA AAGAGATTCC TCGCTGGATC AATCGGCTTG CCTACTTCAG CTCCTGTATA 
CCTTTTCTAC AGAGTTGCCT CCCAAAGGAG TGGCTCACGC CCGCAGCCCT GCAGTCCAGT 
GTCAGCCAGG AGCTCCAGGA CGAACTGGAG GAAGCAGAGG ACGCTGCAGC CTCGGCTTCC 
ATGGCCAGCA CCACCGCGGG CTCCAGACCT GGGCGCCACA CCAAACTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811424.1
CDS	86..676
Translation	MHQELLSFLLRNNVIKYWMNEYTSSIGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNASELGETFKFKEAVVLGSTDFLEDDIEKIVEELGKEYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLAYFSSCIPFLQSCLPKEWLTPAALQSSVSQELQDELEEAEDAAASASMASTTAGSRPGRHTKL

Target ORF information:

RefSeq Version	XM_010813122.3
Organism	Bos taurus(cattle)
Definition	Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813122.3

ORF Insert Sequence:

ATGCATCAGG AACTTCTCTC CTTTTTGTTG AGAAATAATG TAATAAAGTA CTGGATGAAC 
GAATACACCT CTTCCATCGG AATTGGAGTT TTTCATTCAG GAATTGAAGT ATATGGCAGA 
GAATTTGCTT ATGGTGGCCA TCCTTATCCC TTTTCTGGAA TATTTGAAAT TTCCCCAGGA 
AATGCTTCTG AACTAGGAGA AACATTTAAA TTTAAAGAAG CTGTTGTTTT AGGGAGCACT 
GACTTCCTAG AAGATGATAT AGAAAAAATT GTGGAAGAAT TGGGAAAAGA ATACAAAGGC 
AATGCCTATC ATTTGATGCA TAAAAATTGC AATCACTTTT CTTCGGCTTT ATCAGAGATT 
CTCTGTGGGA AAGAGATTCC TCGCTGGATC AATCGGCTTG CCTACTTCAG CTCCTGTATA 
CCTTTTCTAC AGAGTTGCCT CCCAAAGGAG TGGCTCACGC CCGCAGCCCT GCAGTCCAGT 
GTCAGCCAGG AGCTCCAGGA CGAACTGGAG GAAGCAGAGG ACGCTGCAGC CTCGGCTTCC 
ATGGCCAGCA CCACCGCGGG CTCCAGACCT GGGCGCCACA CCAAACTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DESI2 ( XM_010813122.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811424.1 Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa50115
Clone ID Related Accession (Same CDS sequence)	XM_010813122.3 , XM_010813122.2
Accession Version	XM_010813122.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	desumoylating isopeptidase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104436.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813122.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCAGG AACTTCTCTC CTTTTTGTTG AGAAATAATG TAATAAAGTA CTGGATGAAC 
GAATACACCT CTTCCATCGG AATTGGAGTT TTTCATTCAG GAATTGAAGT ATATGGCAGA 
GAATTTGCTT ATGGTGGCCA TCCTTATCCC TTTTCTGGAA TATTTGAAAT TTCCCCAGGA 
AATGCTTCTG AACTAGGAGA AACATTTAAA TTTAAAGAAG CTGTTGTTTT AGGGAGCACT 
GACTTCCTAG AAGATGATAT AGAAAAAATT GTGGAAGAAT TGGGAAAAGA ATACAAAGGC 
AATGCCTATC ATTTGATGCA TAAAAATTGC AATCACTTTT CTTCGGCTTT ATCAGAGATT 
CTCTGTGGGA AAGAGATTCC TCGCTGGATC AATCGGCTTG CCTACTTCAG CTCCTGTATA 
CCTTTTCTAC AGAGTTGCCT CCCAAAGGAG TGGCTCACGC CCGCAGCCCT GCAGTCCAGT 
GTCAGCCAGG AGCTCCAGGA CGAACTGGAG GAAGCAGAGG ACGCTGCAGC CTCGGCTTCC 
ATGGCCAGCA CCACCGCGGG CTCCAGACCT GGGCGCCACA CCAAACTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811424.1
CDS	356..946
Translation	MHQELLSFLLRNNVIKYWMNEYTSSIGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNASELGETFKFKEAVVLGSTDFLEDDIEKIVEELGKEYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLAYFSSCIPFLQSCLPKEWLTPAALQSSVSQELQDELEEAEDAAASASMASTTAGSRPGRHTKL

Target ORF information:

RefSeq Version	XM_010813122.2
Organism	Bos taurus(cattle)
Definition	Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813122.2

ORF Insert Sequence:

ATGCATCAGG AACTTCTCTC CTTTTTGTTG AGAAATAATG TAATAAAGTA CTGGATGAAC 
GAATACACCT CTTCCATCGG AATTGGAGTT TTTCATTCAG GAATTGAAGT ATATGGCAGA 
GAATTTGCTT ATGGTGGCCA TCCTTATCCC TTTTCTGGAA TATTTGAAAT TTCCCCAGGA 
AATGCTTCTG AACTAGGAGA AACATTTAAA TTTAAAGAAG CTGTTGTTTT AGGGAGCACT 
GACTTCCTAG AAGATGATAT AGAAAAAATT GTGGAAGAAT TGGGAAAAGA ATACAAAGGC 
AATGCCTATC ATTTGATGCA TAAAAATTGC AATCACTTTT CTTCGGCTTT ATCAGAGATT 
CTCTGTGGGA AAGAGATTCC TCGCTGGATC AATCGGCTTG CCTACTTCAG CTCCTGTATA 
CCTTTTCTAC AGAGTTGCCT CCCAAAGGAG TGGCTCACGC CCGCAGCCCT GCAGTCCAGT 
GTCAGCCAGG AGCTCCAGGA CGAACTGGAG GAAGCAGAGG ACGCTGCAGC CTCGGCTTCC 
ATGGCCAGCA CCACCGCGGG CTCCAGACCT GGGCGCCACA CCAAACTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DESI2 ( XM_015475048.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330534.1 Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X3, mRNA.

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CloneID	OBa92901
Clone ID Related Accession (Same CDS sequence)	XM_015475048.1
Accession Version	XM_015475048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	desumoylating isopeptidase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104436.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCAGG AACTTCTCTC CTTTTTGTTG AGAAATAATG TAATAAAGTA CTGGATGAAC 
GAATACACCT CTTCCATCGG AATTGGAGTT TTTCATTCAG GAATTGAAGT ATATGGCAGA 
GCTGTTGTTT TAGGGAGCAC TGACTTCCTA GAAGATGATA TAGAAAAAAT TGTGGAAGAA 
TTGGGAAAAG AATACAAAGG CAATGCCTAT CATTTGATGC ATAAAAATTG CAATCACTTT 
TCTTCGGCTT TATCAGAGAT TCTCTGTGGG AAAGAGATTC CTCGCTGGAT CAATCGGCTT 
GCCTACTTCA GCTCCTGTAT ACCTTTTCTA CAGAGTTGCC TCCCAAAGGA GTGGCTCACG 
CCCGCAGCCC TGCAGTCCAG TGTCAGCCAG GAGCTCCAGG ACGAACTGGA GGAAGCAGAG 
GACGCTGCAG CCTCGGCTTC CATGGCCAGC ACCACCGCGG GCTCCAGACC TGGGCGCCAC 
ACCAAACTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330534.1
CDS	363..854
Translation	MHQELLSFLLRNNVIKYWMNEYTSSIGIGVFHSGIEVYGRAVVLGSTDFLEDDIEKIVEELGKEYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLAYFSSCIPFLQSCLPKEWLTPAALQSSVSQELQDELEEAEDAAASASMASTTAGSRPGRHTKL

Target ORF information:

RefSeq Version	XM_015475048.1
Organism	Bos taurus(cattle)
Definition	Bos taurus desumoylating isopeptidase 2 (DESI2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475048.1

ORF Insert Sequence:

ATGCATCAGG AACTTCTCTC CTTTTTGTTG AGAAATAATG TAATAAAGTA CTGGATGAAC 
GAATACACCT CTTCCATCGG AATTGGAGTT TTTCATTCAG GAATTGAAGT ATATGGCAGA 
GCTGTTGTTT TAGGGAGCAC TGACTTCCTA GAAGATGATA TAGAAAAAAT TGTGGAAGAA 
TTGGGAAAAG AATACAAAGG CAATGCCTAT CATTTGATGC ATAAAAATTG CAATCACTTT 
TCTTCGGCTT TATCAGAGAT TCTCTGTGGG AAAGAGATTC CTCGCTGGAT CAATCGGCTT 
GCCTACTTCA GCTCCTGTAT ACCTTTTCTA CAGAGTTGCC TCCCAAAGGA GTGGCTCACG 
CCCGCAGCCC TGCAGTCCAG TGTCAGCCAG GAGCTCCAGG ACGAACTGGA GGAAGCAGAG 
GACGCTGCAG CCTCGGCTTC CATGGCCAGC ACCACCGCGG GCTCCAGACC TGGGCGCCAC 
ACCAAACTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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DESI2 cDNA ORF clone, Bos taurus(cattle)

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