SHANK1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SHANK1
Entrez Gene ID	518970
Full Name	SH3 and multiple ankyrin repeat domains 1
General protein information	Preferred Names SH3 and multiple ankyrin repeat domains 1 Names SH3 and multiple ankyrin repeat domains protein 1 LOW QUALITY PROTEIN: SH3 and multiple ankyrin repeat domains protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002695214.4	XP_002695260.2	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
XM_015467539.1	XP_015323025.1	LOW QUALITY PROTEIN: SH3 and multiple ankyrin repeat domains protein 1 isoform X2
XM_024978739.1	XP_024834507.1	LOW QUALITY PROTEIN: SH3 and multiple ankyrin repeat domains protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SHANK1	XP_005616915.1
Xenopus tropicalis (tropical clawed frog)	shank1	XP_002938582.2
Homo sapiens (human)	SHANK1	NP_057232.2
Macaca mulatta (Rhesus monkey)	LOC719673	XP_002808253.1
Bos taurus (cattle)	SHANK1	XP_002695260.2
Mus musculus (house mouse)	Shank1	NP_001029287.1
Rattus norvegicus (Norway rat)	Shank1	NP_113939.2

Gene Ontology

The following SHANK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHANK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa225297	XM_024978739.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 1 (SHANK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa29074	XM_002695214.4 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 1 (SHANK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa94991	XM_015467539.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 1 (SHANK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHANK1 ( XM_024978739.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834507.1 Bos taurus SH3 and multiple ankyrin repeat domains 1 (SHANK1), mRNA.

Price & Availability↑

CloneID	OBa225297
Clone ID Related Accession (Same CDS sequence)	XM_024978739.1
Accession Version	XM_024978739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: SH3 and multiple ankyrin repeat domains protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGACCCACA GCCCCGCGTC AAGCGAGGAC GAAGAACGCC ACAGTGTCAG CGAGTGTCCC 
GAGGGGGGCT CAGAGTCCGA CAGCTCCCCA GACGGGCCCG GGAGAGGCCC CCGGGGGACC 
CGGGGCCGGG GCAGCGCAGC ACCTGGTAGC CTGGCCTCGA TCCGAGGCCT CCAGAGCCGC 
TCCATGTCCG TCCCAGACGA CGCCCACTTC AGCATGATGG TTTTTAGGAT CGGCATCCCT 
GACCTTCACC AGACAAAATG CCTGCGCTTC AACCCCGATG CCACCATCTG GACGGCCAAG 
CAGCAGGTGC TCTGTGCCCT GAGCGAGAGC CTCCAGGACG TGCTCAACTA TGGCCTGTTC 
CAGCCAGCCA CCTCGGGCCG GGACGCCAAC TTCCTGGAGG AGGAGCGGCT GCTGCGGGAG 
TACCCCCAGT CCTTCGAGAA GGGGGTGCCC TACCTGGAGT TCCGATACAA GACCCGTGTT 
TACAAGCAGA CCAACCTGGA TGAGAAGCAG CTGGCCAAGT TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TGGAGTACGT GCAGCTCGGG ACATCCGATA AGGTGGCACG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA TCATGACTCA GATTCAGGAG AGACCCCCCT GACGCTGGCT 
GCTCAGACCG AAGGCTCCGT AGAGGTGATT CGAACCCTAT GCCTGGGCGG GGCGCACATT 
GACTTCCGGG CCCGGGATGG CATGACCGCG CTACACAAGG CCGCCTGTGC CCGCCACTGC 
CTCGCTCTGA CGGCGCTCCT GGATCTTGGG GGCTCCCCCA ACTACAAGGA CCGCCGAGGG 
CTGACTCCCC TGTTCCACAC GGCTATGGTG GGGGGTGACC CCCGCTGTTG CGAGCTGCTC 
CTGTACAACA GGGCCCAGCT GGGCATAGCT GATGAGAACG GCTGGCAGGA GATTCACCAG 
GCCTGCCAGC GCGGTCACTC TCAGCATCTG GAACATCTGC TCTTCTACGG AGCTGAGCCT 
GGAGCCCAGA ACGCCTCGGG GAACACGGCC CTACACATCT GTGCCCTCTA CAATAAGGAG 
ACGTGTGCTA GGATCCTTCT CTATCGAGGG GCCAACAAGG ATGTCAAGAA TAACAGTGGA 
CAGACCCCCT TCCAGGTGGC AGTGATCGCT GGGAATTTTG AACTCGGGGA GCTGATCCGA 
AACCATCGAG AACAGGACGT GGTGCCCTTC CAGGAGTCCC CCAAATATGC AGCCCGGCGA 
CGGGGACCCC CGGGCACAGG GCTGACGGTG CCCCCCGCAC TGCTGCGGGC CAACAGTGAT 
ACCAGCATGG CCCTGCCCGA CTGGATGGTG TTCGCCGCCC CAGGGACCTC GTCTGCGGGG 
GCCCCCGGCC CTACCGCAGG GCCCCAGGGC CAGGCACAGC CCGCCGCCCC CAGCGCCAAG 
CTCAGCAGCG GGACCCTGCG AAGTGCCAGC AGCCCCCGGG GCGCCCGGGC CCGCTCCCCA 
TCCCGCGGGA GGCACCCTGA GGAGGCCAAG CGCCAGCCCC GTGGACGGCC CAGCTCCAGC 
GGGACACCCC GGGAAGGGCC AGCCGGGGGC ACGGGGGGCT CCGGGGGCCC CGGGGGCTCC 
CTGGGCAGCC GCGGGAGACG CAGGAAGCTC TACTCGGCAG TCCCTGGACG CTCCTTCATG 
GCTGTGAAGT CCTACCAGGC CCAAGCCGAG GGGGAGATCT CCCTGAGCAA GGGCGAAAAG 
ATCAAAGTGC TTAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGCATC 
GGCTGGTTCC CCTCTGACTG CCTGGAAGAG GTGGCCAACC GCTCCCAGGA AGCAAAGCAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCCGGCATT ATACCGTGGG CTCCTACGAC 
AGCTTTGATG CCCCAAGCTT GATGGATGGG ATTGGCCCAG GGAGCGATTA CATCATCAAA 
GAGAAGACAG TCCTGCTGCA GAAGAAGGAC AGTGAGGGGT TTGGGTTCGT GCTCCGGGGT 
GCCAAGGCGC AGACCCCCAT CGAGGAGTTC ACCCCCACCC CAGCCTTCCC GGCACTGCAG 
TATCTGGAGT CAGTGGACGA GGGCGGCGTG GCATGGCGGG CTGGACTGCG AATGGGAGAC 
TTCCTCATCG AGGTGAACGG GCAGAACGTG GTGAAGGTCG GCCACCGGCA GGTGGTGAAC 
ATGATCCGCC AAGGGGGCAA CACGCTGATG GTCAAGGTGG TGATGGTCAC CAGGCACCCG 
GACATGGATG AAGCTGTTCA CAAGAAAGCA CCCCAGCAAG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCT TGCGTTCCAA GTCTATGACC TCTGAGCTTG AGGAGATGGA GTACGAGCAG 
CAGCCGCCCC CGATGCCCAG CATGGAGAAA AAGAGGACTG TGTATCAGAT GGCTCTCAAC 
AAACTGGATG AAATCTTGGC GGCAGCTCAG CAGACTATCA GTGCAAGCGA GAGTCCTGGG 
CCTGGTGGCT TGGCGTCCCT GGGTAAACAC CGACCCAAAG GCTTCTTTAC CACTGAGTCG 
AGCTTTGATC CCCACCACCG CTCCCAGCCA AGTTACGAAC GTCCTTCCTA CCTGCCTCCT 
GGACCCGGGC TCATGCTCCG ACAAAAATCT ATTGGGGCTG CAGAGGACGA TAGACCCTAC 
CTAGCACCCC CAGCCATGAA GTTCAGCCGC AGTCTGTCTG TGCCTGGTTC GGAGGACATT 
CCCCCGCCGC CCACCACGTC CCCACCAGAG CCCCCCTACA GCACGCCTCC AGCCCCATCC 
TCCTCCGGCC GCCTCACTCC CTCTCCGCGG GGTGGGCCCT TCAACCCCAG CTCAGGTGGC 
ACCCACCCGG CCTCGTCCCC GGCGTCCTTC GATGGGCCTG CTCCCCCTGA CACCCGGATA 
GGGAGCCGAG ATAAGAGCCT GTATCACAGC GGGCCCCTGC CCCCACCCCA CCACCACCCG 
CCCCACCACC ACCACCACCA CGCCCCGCCC CCCCAGCCCC ACCACCACCA TGCCCACCCG 
CCTCACGCCC CGGAGATGGA GACTGGCGGC TCCCCCGACG ACCCCCCACC CCGCCTGGCT 
CTGGGGCCCC AGCCCAGCCT ACGAGGCTGG CGGGGCGGCG GGCCCAGCCC GACCCCGGGT 
GCTCTGTCCC CGTCGCACCA CGGCAGCGGG GGCGGCGCGG GCGGCCCCTC CCAGGCTCCG 
GCGCTGCGCT ACTTCCAGCT GCCTCCGCGG GCGGCCAGCG CGGCCATGTA TGTGCCCGCC 
CGTTCCGGCC GCGGGCGCAA GGGCCCGCTG GTCAAGCAGA CCAAAGTCGT CGAAGGCGAG 
CCCCAGAAGG GCGGCGGCCT CCCGCCTGCC CCCTCGCCCA CGTCTCCGGC GTCCCCGCAG 
CCGCCGCCGC CGCCGGCCGC GGCCGCCCCG TCGGAAAAGA ACTCCATCCC CATCCCCACC 
ATCATCATCA AGGCGCCATC CACCAGTAGC AGCGGCCGCA GCAGCCAGGG GAGCAGCACC 
GAGGCGGAGC CCCCGAGCCA GCCCGAGGCC GCGGGCGGCG GGGTCGGCGG CGGCGGCTCT 
TCCGCGCCCA GCCCCGCCCC GGCCGTGTCG CCCGTGCCGC CGTCCCCCTC GCCTGTGCCC 
ACCCCCGCCT CGCCCAGCGG CCCGGCCACC CTGGATTTCA CCAGCCAGTT CGGAGCGGCC 
CTGGTGGGGG CGGCTCGGAG GGAAGGGGGC TGGCAGAACG AAGCCCGCCG GCGGTCCACG 
CTCTTCCTCT CCACCGACGC GGGGGACGAG GACGGCGGCG ACAGCTCGCT GGGCCCAGGG 
GCGCCCCCGG GCCCCCGGCT GCGCCACTCC AAGTCCATCG ACGAAGGCAT GTTTTCGGCC 
GAGCCCTACC TCCGGCTGGA GTCGGCGGGC GCCGGCAGCG GCTACGCGGG CTACGCGGCC 
GGCGGCCGAG CCTACGGGGG CAGCAGCGCC TTCACCAGCT TCCTGCCGCC GCGACCCCTG 
GTCCACCCGC TGACCGGCAA GNGCCTGGAC CCGGCCTCCC CACTCGGGCT GGCCCTGGCC 
GCCCGGGAGC GGGCGCTGAA GGAGTCCTCG GAGGGCGGTG GGCCCCCCCA GCCCCCTCCA 
AGGCCCCCAT CGCCCCGCTA CGAGGCCCCG CCGCCCACCC CGCACCACCA CTCGCCCCAC 
GCCCACCACG AGCCAGTGCT GCGCCTCTGG GGAGCCTCGC CCCCGGACCC GGCCCGCCGG 
GAGCTGGGGT ACCGGGCAGG GCTGGGCAGC CAGGAGAAGT CCCTTCCGGC CAGCCCACCG 
GCGGCCCGAC GCTCCTTGCT GCACCGCCTG CCTCCCACAG CTCCCGGGGT CGGGCCCCTC 
CTGCTGCAGC TGGGGCCCGA GCCCCCCGCC CCGCACCCCG GGGTAAGCAA GCCCTGGAGA 
TCTGGCGCCC CCCCGGAGGA GCCCGAGCGG CTGCCCCTCC ACGTGCGGTT CCTGGAGAAC 
TGCCAGCCCC GGGCCGGCGG GGCGGGTGGA AGGGGGCCCC CCTCGGAGGA CGGGCCGGGG 
GTCCCGCCGC CCAGCCCGCG CCGCTCTGTG CCGCCCTCGC CGACCTCCCC GCGGGGCAGT 
GAAGAGAACG GGCTCCCCCT GCTGGTCCTG CCGCCGCCCG CCCCCTCAGT AGACGTGGAA 
GACGGCGAAT TCCTCTTTGT GGAACCGCTG CCCCCGCCCC TCGAGTTTTC CAACAGCTTC 
GAGAAGCCGG AGTCGCCTCT CACGCCGGGG CCTCCCCACC CGCTGCCGGA CCCACCCACC 
CCGACCACCC CATTGCCCTC TGTGCCGCCC CCGGCCGTGG CCGCCGCCCC TCCCACCCTG 
GACTCGACCG CATCCAGCCT GACTTCCTAT GACAGCGAGG TGGCAACGCT GACCCAGGGG 
GCCCCCGCGG CTCCTGGGGA CCCCCCTCCG CCAGGCCCTC CCGCCCCAGC CGCTCCGGCT 
CCCCCAGCCC CCCAGCCTGG CCCAGACCCT CCCCCGGGCA CGGATTCTGG TATTGAGGAG 
GTGGACAGTC GGAGCAGCAG CGACCACCCC CTGGAGACCA TCAGCAGCGC CTCCACGCTG 
AGCAGCCTGT CCGCTGAAGG CGGGGGCGGC GCAGGGGGTG GAACTGGCGG CGTTGGAGGC 
AGTGGTGGCG CCAGCGTGGC CGGTGGACCA GAGCTCCTGG ATACCTACGT GGCCTACTTG 
GATGGCCAGG CCTTCGGGGG CAGCGGCACT CCTGGCCCCC CATACCCCCC GCAGCTCATG 
ACTCCCTCTA AGCTCCGGGG CCGGGCGCTC GGGGCCGGCG GAGGCCTGCG GCCGGGCCCC 
GGTGGGGGAC TCCGAGACCC TATCACTCCC ACCAGCCCCA CTGTCTCGGT GACGGGGGCG 
GGAACCGATG GGCTGCTGGC CCTCAGCGGT TGTCCGGGAT CCTCGACGGC AGGTGTGGCT 
GGCGGCCCCG TGGCTGTAGA GCCGGAAGGT CCGCCAGTGC CCTTGCCGTC GGCCTCCTCC 
CTCCCTCGAA AGCTGCTGCC CTGGGAGGAG GGCCCGGGCC CGCCGCCGCC CCCCCTGCCG 
GGGCCCTTAG CCCAACCTCA GGCCTCAGCC TTGGCCACTG TAAAAGCCAG CATCATCAGT 
GAACTCAGCT CCAAGCTTCA GCAGTTTGGG GGCTCCTCGG CAGCTGGTGG CGCTCTGCCC 
TGGGCCCGGG GAGGCAGCGG GGGAAGCGGG GACAGCCACC ACGGGGGAGC CAGCTACGTC 
CCCGAGCGGA CTTCCTCCCT GCAGCGGCAG AGACTCTCCG AAGACTCCCA GTCCTCGCTC 
CTCTCCAAAC CCGTGAGCAG CCTGTTTCAG AACTGGTCCA AACCACCTCT GCCGCCACTC 
CCCACGGGAA CCGCGGTGTC CCCTTCAGCC GCTGCAGCCC CGGGGGCCAC CTCCCCATCG 
GCTTCCTCCT CCTCCACGTC CGCCCGCCAC CTCCAGGGCG TGGAGTTCGA GATGCGCCCA 
CCTTTGCTCC GCCGGGCCCC CAGCCCCTCA TTGCTGCCCG CCTCGGAGCA CAAGGTCAGC 
CCTGCGCCCC GGCCCTCGTC CCTACCCATC CTGCCTTCCG GACCCCTCTA CCCGGGCCTC 
TTTGACATCC GAGGCTCCCC CACCGGAGGG GCAGGAGGCT CGGCCGACCC CTTTGCCCCC 
GTCTTTGTAC CGCCGCACCC CGGGATATCC GGGGGTCTCG GCGGGGCCTT GTCAGGGGCC 
TCCCGCTCCC TCTCACCGAC CCGCCTGCTT TCCCTGCCCC CGGACAAGCC GTTCGGCGCT 
AAACCTCTGG GCTTCTGGAC CAAGTTCGAT GTGGCTGACT GGCTAGAGTG GCTGGGCTTG 
GCCGAACACC GGGCCCGGTT CCTGGACCAC GAGATCGATG GCTCCCACCT TCCCGCCTTG 
ACCAAGGAGG ACTACGTCGA TTTGGGGGTG ACCAGGGTGG GACACCGCAT GAACATCGAC 
CGGGCTCTCA AGTTTTTCCT GGAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834507.1
CDS	1..6510
Translation	MTHSPASSEDEERHSVSECPEGGSESDSSPDGPGRGPRGTRGRGSAAPGSLASIRGLQSRSMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELLLYNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKETCARILLYRGANKDVKNNSGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARRRGPPGTGLTVPPALLRANSDTSMALPDWMVFAAPGTSSAGAPGPTAGPQGQAQPAAPSAKLSSGTLRSASSPRGARARSPSRGRHPEEAKRQPRGRPSSSGTPREGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFMAVKSYQAQAEGEISLSKGEKIKVLSIGEGGFWEGQVKGRIGWFPSDCLEEVANRSQEAKQESRSDKAKRLFRHYTVGSYDSFDAPSLMDGIGPGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKAPQQAKRLPPPAISLRSKSMTSELEEMEYEQQPPPMPSMEKKRTVYQMALNKLDEILAAAQQTISASESPGPGGLASLGKHRPKGFFTTESSFDPHHRSQPSYERPSYLPPGPGLMLRQKSIGAAEDDRPYLAPPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPAPSSSGRLTPSPRGGPFNPSSGGTHPASSPASFDGPAPPDTRIGSRDKSLYHSGPLPPPHHHPPHHHHHHAPPPQPHHHHAHPPHAPEMETGGSPDDPPPRLALGPQPSLRGWRGGGPSPTPGALSPSHHGSGGGAGGPSQAPALRYFQLPPRAASAAMYVPARSGRGRKGPLVKQTKVVEGEPQKGGGLPPAPSPTSPASPQPPPPPAAAAPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPSQPEAAGGGVGGGGSSAPSPAPAVSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLSTDAGDEDGGDSSLGPGAPPGPRLRHSKSIDEGMFSAEPYLRLESAGAGSGYAGYAAGGRAYGGSSAFTSFLPPRPLVHPLTGKnullLDPASPLGLALAARERALKESSEGGGPPQPPPRPPSPRYEAPPPTPHHHSPHAHHEPVLRLWGASPPDPARRELGYRAGLGSQEKSLPASPPAARRSLLHRLPPTAPGVGPLLLQLGPEPPAPHPGVSKPWRSGAPPEEPERLPLHVRFLENCQPRAGGAGGRGPPSEDGPGVPPPSPRRSVPPSPTSPRGSEENGLPLLVLPPPAPSVDVEDGEFLFVEPLPPPLEFSNSFEKPESPLTPGPPHPLPDPPTPTTPLPSVPPPAVAAAPPTLDSTASSLTSYDSEVATLTQGAPAAPGDPPPPGPPAPAAPAPPAPQPGPDPPPGTDSGIEEVDSRSSSDHPLETISSASTLSSLSAEGGGGAGGGTGGVGGSGGASVAGGPELLDTYVAYLDGQAFGGSGTPGPPYPPQLMTPSKLRGRALGAGGGLRPGPGGGLRDPITPTSPTVSVTGAGTDGLLALSGCPGSSTAGVAGGPVAVEPEGPPVPLPSASSLPRKLLPWEEGPGPPPPPLPGPLAQPQASALATVKASIISELSSKLQQFGGSSAAGGALPWARGGSGGSGDSHHGGASYVPERTSSLQRQRLSEDSQSSLLSKPVSSLFQNWSKPPLPPLPTGTAVSPSAAAAPGATSPSASSSSTSARHLQGVEFEMRPPLLRRAPSPSLLPASEHKVSPAPRPSSLPILPSGPLYPGLFDIRGSPTGGAGGSADPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKPLGFWTKFDVADWLEWLGLAEHRARFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLER

Target ORF information:

RefSeq Version	XM_024978739.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 1 (SHANK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978739.1

ORF Insert Sequence:

ATGACCCACA GCCCCGCGTC AAGCGAGGAC GAAGAACGCC ACAGTGTCAG CGAGTGTCCC 
GAGGGGGGCT CAGAGTCCGA CAGCTCCCCA GACGGGCCCG GGAGAGGCCC CCGGGGGACC 
CGGGGCCGGG GCAGCGCAGC ACCTGGTAGC CTGGCCTCGA TCCGAGGCCT CCAGAGCCGC 
TCCATGTCCG TCCCAGACGA CGCCCACTTC AGCATGATGG TTTTTAGGAT CGGCATCCCT 
GACCTTCACC AGACAAAATG CCTGCGCTTC AACCCCGATG CCACCATCTG GACGGCCAAG 
CAGCAGGTGC TCTGTGCCCT GAGCGAGAGC CTCCAGGACG TGCTCAACTA TGGCCTGTTC 
CAGCCAGCCA CCTCGGGCCG GGACGCCAAC TTCCTGGAGG AGGAGCGGCT GCTGCGGGAG 
TACCCCCAGT CCTTCGAGAA GGGGGTGCCC TACCTGGAGT TCCGATACAA GACCCGTGTT 
TACAAGCAGA CCAACCTGGA TGAGAAGCAG CTGGCCAAGT TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TGGAGTACGT GCAGCTCGGG ACATCCGATA AGGTGGCACG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA TCATGACTCA GATTCAGGAG AGACCCCCCT GACGCTGGCT 
GCTCAGACCG AAGGCTCCGT AGAGGTGATT CGAACCCTAT GCCTGGGCGG GGCGCACATT 
GACTTCCGGG CCCGGGATGG CATGACCGCG CTACACAAGG CCGCCTGTGC CCGCCACTGC 
CTCGCTCTGA CGGCGCTCCT GGATCTTGGG GGCTCCCCCA ACTACAAGGA CCGCCGAGGG 
CTGACTCCCC TGTTCCACAC GGCTATGGTG GGGGGTGACC CCCGCTGTTG CGAGCTGCTC 
CTGTACAACA GGGCCCAGCT GGGCATAGCT GATGAGAACG GCTGGCAGGA GATTCACCAG 
GCCTGCCAGC GCGGTCACTC TCAGCATCTG GAACATCTGC TCTTCTACGG AGCTGAGCCT 
GGAGCCCAGA ACGCCTCGGG GAACACGGCC CTACACATCT GTGCCCTCTA CAATAAGGAG 
ACGTGTGCTA GGATCCTTCT CTATCGAGGG GCCAACAAGG ATGTCAAGAA TAACAGTGGA 
CAGACCCCCT TCCAGGTGGC AGTGATCGCT GGGAATTTTG AACTCGGGGA GCTGATCCGA 
AACCATCGAG AACAGGACGT GGTGCCCTTC CAGGAGTCCC CCAAATATGC AGCCCGGCGA 
CGGGGACCCC CGGGCACAGG GCTGACGGTG CCCCCCGCAC TGCTGCGGGC CAACAGTGAT 
ACCAGCATGG CCCTGCCCGA CTGGATGGTG TTCGCCGCCC CAGGGACCTC GTCTGCGGGG 
GCCCCCGGCC CTACCGCAGG GCCCCAGGGC CAGGCACAGC CCGCCGCCCC CAGCGCCAAG 
CTCAGCAGCG GGACCCTGCG AAGTGCCAGC AGCCCCCGGG GCGCCCGGGC CCGCTCCCCA 
TCCCGCGGGA GGCACCCTGA GGAGGCCAAG CGCCAGCCCC GTGGACGGCC CAGCTCCAGC 
GGGACACCCC GGGAAGGGCC AGCCGGGGGC ACGGGGGGCT CCGGGGGCCC CGGGGGCTCC 
CTGGGCAGCC GCGGGAGACG CAGGAAGCTC TACTCGGCAG TCCCTGGACG CTCCTTCATG 
GCTGTGAAGT CCTACCAGGC CCAAGCCGAG GGGGAGATCT CCCTGAGCAA GGGCGAAAAG 
ATCAAAGTGC TTAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGCATC 
GGCTGGTTCC CCTCTGACTG CCTGGAAGAG GTGGCCAACC GCTCCCAGGA AGCAAAGCAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCCGGCATT ATACCGTGGG CTCCTACGAC 
AGCTTTGATG CCCCAAGCTT GATGGATGGG ATTGGCCCAG GGAGCGATTA CATCATCAAA 
GAGAAGACAG TCCTGCTGCA GAAGAAGGAC AGTGAGGGGT TTGGGTTCGT GCTCCGGGGT 
GCCAAGGCGC AGACCCCCAT CGAGGAGTTC ACCCCCACCC CAGCCTTCCC GGCACTGCAG 
TATCTGGAGT CAGTGGACGA GGGCGGCGTG GCATGGCGGG CTGGACTGCG AATGGGAGAC 
TTCCTCATCG AGGTGAACGG GCAGAACGTG GTGAAGGTCG GCCACCGGCA GGTGGTGAAC 
ATGATCCGCC AAGGGGGCAA CACGCTGATG GTCAAGGTGG TGATGGTCAC CAGGCACCCG 
GACATGGATG AAGCTGTTCA CAAGAAAGCA CCCCAGCAAG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCT TGCGTTCCAA GTCTATGACC TCTGAGCTTG AGGAGATGGA GTACGAGCAG 
CAGCCGCCCC CGATGCCCAG CATGGAGAAA AAGAGGACTG TGTATCAGAT GGCTCTCAAC 
AAACTGGATG AAATCTTGGC GGCAGCTCAG CAGACTATCA GTGCAAGCGA GAGTCCTGGG 
CCTGGTGGCT TGGCGTCCCT GGGTAAACAC CGACCCAAAG GCTTCTTTAC CACTGAGTCG 
AGCTTTGATC CCCACCACCG CTCCCAGCCA AGTTACGAAC GTCCTTCCTA CCTGCCTCCT 
GGACCCGGGC TCATGCTCCG ACAAAAATCT ATTGGGGCTG CAGAGGACGA TAGACCCTAC 
CTAGCACCCC CAGCCATGAA GTTCAGCCGC AGTCTGTCTG TGCCTGGTTC GGAGGACATT 
CCCCCGCCGC CCACCACGTC CCCACCAGAG CCCCCCTACA GCACGCCTCC AGCCCCATCC 
TCCTCCGGCC GCCTCACTCC CTCTCCGCGG GGTGGGCCCT TCAACCCCAG CTCAGGTGGC 
ACCCACCCGG CCTCGTCCCC GGCGTCCTTC GATGGGCCTG CTCCCCCTGA CACCCGGATA 
GGGAGCCGAG ATAAGAGCCT GTATCACAGC GGGCCCCTGC CCCCACCCCA CCACCACCCG 
CCCCACCACC ACCACCACCA CGCCCCGCCC CCCCAGCCCC ACCACCACCA TGCCCACCCG 
CCTCACGCCC CGGAGATGGA GACTGGCGGC TCCCCCGACG ACCCCCCACC CCGCCTGGCT 
CTGGGGCCCC AGCCCAGCCT ACGAGGCTGG CGGGGCGGCG GGCCCAGCCC GACCCCGGGT 
GCTCTGTCCC CGTCGCACCA CGGCAGCGGG GGCGGCGCGG GCGGCCCCTC CCAGGCTCCG 
GCGCTGCGCT ACTTCCAGCT GCCTCCGCGG GCGGCCAGCG CGGCCATGTA TGTGCCCGCC 
CGTTCCGGCC GCGGGCGCAA GGGCCCGCTG GTCAAGCAGA CCAAAGTCGT CGAAGGCGAG 
CCCCAGAAGG GCGGCGGCCT CCCGCCTGCC CCCTCGCCCA CGTCTCCGGC GTCCCCGCAG 
CCGCCGCCGC CGCCGGCCGC GGCCGCCCCG TCGGAAAAGA ACTCCATCCC CATCCCCACC 
ATCATCATCA AGGCGCCATC CACCAGTAGC AGCGGCCGCA GCAGCCAGGG GAGCAGCACC 
GAGGCGGAGC CCCCGAGCCA GCCCGAGGCC GCGGGCGGCG GGGTCGGCGG CGGCGGCTCT 
TCCGCGCCCA GCCCCGCCCC GGCCGTGTCG CCCGTGCCGC CGTCCCCCTC GCCTGTGCCC 
ACCCCCGCCT CGCCCAGCGG CCCGGCCACC CTGGATTTCA CCAGCCAGTT CGGAGCGGCC 
CTGGTGGGGG CGGCTCGGAG GGAAGGGGGC TGGCAGAACG AAGCCCGCCG GCGGTCCACG 
CTCTTCCTCT CCACCGACGC GGGGGACGAG GACGGCGGCG ACAGCTCGCT GGGCCCAGGG 
GCGCCCCCGG GCCCCCGGCT GCGCCACTCC AAGTCCATCG ACGAAGGCAT GTTTTCGGCC 
GAGCCCTACC TCCGGCTGGA GTCGGCGGGC GCCGGCAGCG GCTACGCGGG CTACGCGGCC 
GGCGGCCGAG CCTACGGGGG CAGCAGCGCC TTCACCAGCT TCCTGCCGCC GCGACCCCTG 
GTCCACCCGC TGACCGGCAA GNGCCTGGAC CCGGCCTCCC CACTCGGGCT GGCCCTGGCC 
GCCCGGGAGC GGGCGCTGAA GGAGTCCTCG GAGGGCGGTG GGCCCCCCCA GCCCCCTCCA 
AGGCCCCCAT CGCCCCGCTA CGAGGCCCCG CCGCCCACCC CGCACCACCA CTCGCCCCAC 
GCCCACCACG AGCCAGTGCT GCGCCTCTGG GGAGCCTCGC CCCCGGACCC GGCCCGCCGG 
GAGCTGGGGT ACCGGGCAGG GCTGGGCAGC CAGGAGAAGT CCCTTCCGGC CAGCCCACCG 
GCGGCCCGAC GCTCCTTGCT GCACCGCCTG CCTCCCACAG CTCCCGGGGT CGGGCCCCTC 
CTGCTGCAGC TGGGGCCCGA GCCCCCCGCC CCGCACCCCG GGGTAAGCAA GCCCTGGAGA 
TCTGGCGCCC CCCCGGAGGA GCCCGAGCGG CTGCCCCTCC ACGTGCGGTT CCTGGAGAAC 
TGCCAGCCCC GGGCCGGCGG GGCGGGTGGA AGGGGGCCCC CCTCGGAGGA CGGGCCGGGG 
GTCCCGCCGC CCAGCCCGCG CCGCTCTGTG CCGCCCTCGC CGACCTCCCC GCGGGGCAGT 
GAAGAGAACG GGCTCCCCCT GCTGGTCCTG CCGCCGCCCG CCCCCTCAGT AGACGTGGAA 
GACGGCGAAT TCCTCTTTGT GGAACCGCTG CCCCCGCCCC TCGAGTTTTC CAACAGCTTC 
GAGAAGCCGG AGTCGCCTCT CACGCCGGGG CCTCCCCACC CGCTGCCGGA CCCACCCACC 
CCGACCACCC CATTGCCCTC TGTGCCGCCC CCGGCCGTGG CCGCCGCCCC TCCCACCCTG 
GACTCGACCG CATCCAGCCT GACTTCCTAT GACAGCGAGG TGGCAACGCT GACCCAGGGG 
GCCCCCGCGG CTCCTGGGGA CCCCCCTCCG CCAGGCCCTC CCGCCCCAGC CGCTCCGGCT 
CCCCCAGCCC CCCAGCCTGG CCCAGACCCT CCCCCGGGCA CGGATTCTGG TATTGAGGAG 
GTGGACAGTC GGAGCAGCAG CGACCACCCC CTGGAGACCA TCAGCAGCGC CTCCACGCTG 
AGCAGCCTGT CCGCTGAAGG CGGGGGCGGC GCAGGGGGTG GAACTGGCGG CGTTGGAGGC 
AGTGGTGGCG CCAGCGTGGC CGGTGGACCA GAGCTCCTGG ATACCTACGT GGCCTACTTG 
GATGGCCAGG CCTTCGGGGG CAGCGGCACT CCTGGCCCCC CATACCCCCC GCAGCTCATG 
ACTCCCTCTA AGCTCCGGGG CCGGGCGCTC GGGGCCGGCG GAGGCCTGCG GCCGGGCCCC 
GGTGGGGGAC TCCGAGACCC TATCACTCCC ACCAGCCCCA CTGTCTCGGT GACGGGGGCG 
GGAACCGATG GGCTGCTGGC CCTCAGCGGT TGTCCGGGAT CCTCGACGGC AGGTGTGGCT 
GGCGGCCCCG TGGCTGTAGA GCCGGAAGGT CCGCCAGTGC CCTTGCCGTC GGCCTCCTCC 
CTCCCTCGAA AGCTGCTGCC CTGGGAGGAG GGCCCGGGCC CGCCGCCGCC CCCCCTGCCG 
GGGCCCTTAG CCCAACCTCA GGCCTCAGCC TTGGCCACTG TAAAAGCCAG CATCATCAGT 
GAACTCAGCT CCAAGCTTCA GCAGTTTGGG GGCTCCTCGG CAGCTGGTGG CGCTCTGCCC 
TGGGCCCGGG GAGGCAGCGG GGGAAGCGGG GACAGCCACC ACGGGGGAGC CAGCTACGTC 
CCCGAGCGGA CTTCCTCCCT GCAGCGGCAG AGACTCTCCG AAGACTCCCA GTCCTCGCTC 
CTCTCCAAAC CCGTGAGCAG CCTGTTTCAG AACTGGTCCA AACCACCTCT GCCGCCACTC 
CCCACGGGAA CCGCGGTGTC CCCTTCAGCC GCTGCAGCCC CGGGGGCCAC CTCCCCATCG 
GCTTCCTCCT CCTCCACGTC CGCCCGCCAC CTCCAGGGCG TGGAGTTCGA GATGCGCCCA 
CCTTTGCTCC GCCGGGCCCC CAGCCCCTCA TTGCTGCCCG CCTCGGAGCA CAAGGTCAGC 
CCTGCGCCCC GGCCCTCGTC CCTACCCATC CTGCCTTCCG GACCCCTCTA CCCGGGCCTC 
TTTGACATCC GAGGCTCCCC CACCGGAGGG GCAGGAGGCT CGGCCGACCC CTTTGCCCCC 
GTCTTTGTAC CGCCGCACCC CGGGATATCC GGGGGTCTCG GCGGGGCCTT GTCAGGGGCC 
TCCCGCTCCC TCTCACCGAC CCGCCTGCTT TCCCTGCCCC CGGACAAGCC GTTCGGCGCT 
AAACCTCTGG GCTTCTGGAC CAAGTTCGAT GTGGCTGACT GGCTAGAGTG GCTGGGCTTG 
GCCGAACACC GGGCCCGGTT CCTGGACCAC GAGATCGATG GCTCCCACCT TCCCGCCTTG 
ACCAAGGAGG ACTACGTCGA TTTGGGGGTG ACCAGGGTGG GACACCGCAT GAACATCGAC 
CGGGCTCTCA AGTTTTTCCT GGAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK1 ( XM_002695214.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002695260.2 Bos taurus SH3 and multiple ankyrin repeat domains 1 (SHANK1), transcript variant X1, mRNA.

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CloneID	OBa29074
Clone ID Related Accession (Same CDS sequence)	XM_002695214.4
Accession Version	XM_002695214.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002695214.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGACCCACA GCCCCGCGTC AAGCGAGGAC GAAGAACGCC ACAGTGTCAG CGAGTGTCCC 
GAGGGGGGCT CAGAGTCCGA CAGCTCCCCA GACGGGCCCG GGAGAGGCCC CCGGGGGACC 
CGGGGCCGGG GCAGCGCAGC ACCTGGTAGC CTGGCCTCGA TCCGAGGCCT CCAGAGCCGC 
TCCATGTCCG TCCCAGACGA CGCCCACTTC AGCATGATGG TTTTTAGGAT CGGCATCCCT 
GACCTTCACC AGACAAAATG CCTGCGCTTC AACCCCGATG CCACCATCTG GACGGCCAAG 
CAGCAGGTGC TCTGTGCCCT GAGCGAGAGC CTCCAGGACG TGCTCAACTA TGGCCTGTTC 
CAGCCAGCCA CCTCGGGCCG GGACGCCAAC TTCCTGGAGG AGGAGCGGCT GCTGCGGGAG 
TACCCCCAGT CCTTCGAGAA GGGGGTGCCC TACCTGGAGT TCCGATACAA GACCCGTGTT 
TACAAGCAGA CCAACCTGGA TGAGAAGCAG CTGGCCAAGT TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TGGAGTACGT GCAGCTCGGG ACATCCGATA AGGTGGCACG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA TCATGACTCA GATTCAGGAG AGACCCCCCT GACGCTGGCT 
GCTCAGACCG AAGGCTCCGT AGAGGTGATT CGAACCCTAT GCCTGGGCGG GGCGCACATT 
GACTTCCGGG CCCGGGATGG CATGACCGCG CTACACAAGG CCGCCTGTGC CCGCCACTGC 
CTCGCTCTGA CGGCGCTCCT GGATCTTGGG GGCTCCCCCA ACTACAAGGA CCGCCGAGGG 
CTGACTCCCC TGTTCCACAC GGCTATGGTG GGGGGTGACC CCCGCTGTTG CGAGCTGCTC 
CTGTACAACA GGGCCCAGCT GGGCATAGCT GATGAGAACG GCTGGCAGGA GATTCACCAG 
GCCTGCCAGC GCGGTCACTC TCAGCATCTG GAACATCTGC TCTTCTATGG AGCTGAGCCT 
GGAGCCCAGA ACGCCTCGGG GAACACGGCC CTACACATCT GTGCCCTCTA CAATAAGGAG 
ACGTGTGCTA GGATCCTTCT CTATCGAGGG GCCAACAAGG ATGTCAAGAA TAACAGTGGA 
CAGACCCCCT TCCAGGTGGC AGTGATCGCT GGGAATTTTG AACTCGGGGA GCTGATCCGA 
AACCATCGAG AACAGGACGT GGTGCCCTTC CAGGAGTCCC CCAAATATGC AGCCCGGCGA 
CGGGGACCCC CGGGCACAGG GCTGACGGTG CCCCCCGCAC TGCTGCGGGC CAACAGTGAT 
ACCAGCATGG CCCTGCCCGA CTGGATGGTG TTCGCCGCCC CAGGGACCTC GTCTGCGGGG 
GCCCCCGGCC CCACCGCAGG GCCCCAGGGC CAGGCACAGC CCGCCGCCCC CAGCGCCAAG 
CTCAGCAGCG GGACCCTGCG AAGTGCCAGC AGCCCCCGGG GCGCCCGGGC CCGCTCCCCA 
TCCCGAGGGA GGCACCCTGA GGAGGCCAAG CGCCAGCCCC GTGGACGGCC CAGCTCCAGC 
GGGACACCCC GGGAAGGGCC AGCCGGGGGC ACGGGGGGCT CCGGGGGCCC CGGGGGCTCC 
CTGGGCAGCC GCGGGAGACG CAGGAAGCTC TACTCGGCAG TCCCTGGACG CTCCTTCATG 
GCTGTGAAGT CCTACCAGGC CCAAGCCGAG GGGGAGATCT CCCTGAGCAA GGGCGAAAAG 
ATCAAAGTGC TTAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGCATC 
GGCTGGTTCC CCTCTGACTG CCTGGAAGAG GTGGCCAACC GCTCCCAGGA AGCAAAGCAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCCGGCATT ATACCGTGGG CTCCTACGAC 
AGCTTTGATG CCCCAAGCTT GATGGATGGG ATTGGCCCAG GGAGCGATTA CATCATCAAA 
GAGAAGACAG TCCTGCTGCA GAAGAAGGAC AGTGAGGGGT TTGGGTTCGT GCTCCGGGGT 
GCCAAGGCGC AGACCCCCAT CGAGGAGTTC ACCCCCACCC CAGCCTTCCC GGCACTGCAG 
TATCTGGAGT CAGTGGACGA GGGCGGCGTG GCATGGCGGG CTGGACTGCG AATGGGAGAC 
TTCCTCATCG AGGTGAACGG GCAGAACGTG GTGAAGGTCG GCCACCGGCA GGTGGTGAAC 
ATGATCCGCC AAGGGGGCAA CACGCTGATG GTCAAGGTGG TGATGGTCAC CAGGCACCCG 
GACATGGATG AAGCTGTTCA CAAGAAAGCA CCCCAGCAAG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCT TGCGTTCCAA GTCTATGACC TCTGAGCTGG AGGAGATGGA GTACGAGCAG 
CAGCCGCCCC CGATGCCCAG CATGGAGAAA AAGAGGACTG TGTATCAGAT GGCTCTCAAC 
AAACTGGATG AAATCTTGGC GGCAGCTCAG CAGACTATCA GTGCAAGCGA GAGTCCTGGG 
CCTGGTGGCT TGGCGTCCCT GGGTAAACAC CGACCCAAAG GCTTCTTTAC CACTGAGTCG 
AGCTTTGATC CCCACCACCG CTCCCAGCCA AGTTACGAAC GTCCTTCCTA CCTGCCTCCT 
GGACCCGGGC TCATGCTCCG ACAAAAATCT ATTGGGGCTG CAGAGGACGA TAGACCCTAC 
CTAGCACCCC CAGCCATGAA GTTCAGCCGC AGTCTGTCTG TGCCTGGTTC GGAGGACATT 
CCCCCGCCGC CCACCACGTC CCCACCAGAG CCCCCCTACA GCACGCCTCC AGCCCCATCC 
TCCTCCGGCC GCCTCACTCC CTCTCCGCGG GGTGGGCCCT TCAACCCCAG CTCAGGTGGC 
ACCCACCCGG CCTCGTCCCC GGCGTCCTTC GATGGGCCTG CTCCCCCTGA CACCCGGATA 
GGGAGCCGAG ATAAGAGCCT GTATCACAGC GGGCCCCTGC CCCCACCCCA CCACCACCCG 
CCCCACCACC ACCACCACCA CGCCCCGCCC CCCCAGCCCC ACCACCACCA TGCCCACCCG 
CCTCACGCCC CGGAGATGGA GACTGGCGGC TCCCCCGACG ACCCCCCACC CCGCCTGGCT 
CTGGGGCCCC AGCCCAGCCT ACGAGGCTGG CGGGGCGCGG CCGCCCCGTC GGAAAAGAAC 
TCCATCCCCA TCCCCACCAT CATCATCAAG GCGCCGTCCA CCAGTAGCAG CGGCCGCAGC 
AGCCAGGGGA GCAGCACCGA GGCGGAGCCC CCGAGCCAGC CCGAGGCCGC GGGCGGCGGG 
GTCGGCGGCG GCGGCTCTTC CGCGCCCAGC CCCGCCCCGG CCGTGTCGCC CGTGCCGCCG 
TCCCCCTCGC CTGTGCCCAC CCCCGCCTCG CCCAGCGGCC CGGCCACCCT GGATTTCACC 
AGCCAGTTCG GAGCGGCCCT GGTGGGGGCG GCTCGGAGGG AAGGGGGCTG GCAGAACGAA 
GCCCGCCGGC GGTCCACGCT CTTCCTCTCC ACCGACGCGG GGGACGAGGA CGGCGGCGAC 
AGCTCGCTGG GCCCAGGGGC GCCCCCGGGC CCCCGGCTGC GCCACTCCAA GTCCATCGAC 
GAAGGCATGT TTTCGGCCGA GCCCTACCTC CGGCTGGAGT CGGCGGGCGC CGGCAGCGGC 
TACGCGGGCT ACGCGGCCGG CGGCCGAGCC TACGGGGGCA GCAGCGCCTT CACCAGCTTC 
CTGCCGCCGC GACCCCTGGT CCACCCGCTG ACCGGCAAGG CCTTGGACCC GGCCTCCCCA 
CTCGGGCTGG CCCTGGCCGC CCGGGAGCGG GCGCTGAAGG AGTCCTCGGA GGGCGGTGGG 
CCCCCCCAGC CCCCTCCAAG GCCCCCATCG CCCCGCTACG AGGCCCCGCC GCCCACCCCG 
CACCACCACT CGCCCCACGC CCACCACGAG CCAGTGCTGC GCCTCTGGGG AGCCTCGCCC 
CCGGACCCGG CCCGCCGGGA GCTGGGGTAC CGGGCAGGGC TGGGCAGCCA GGAGAAGTCC 
CTTCCGGCCA GCCCACCGGC GGCCCGACGC TCCTTGCTGC ACCGCCTGCC TCCCACAGCT 
CCCGGGGTCG GGCCCCTCCT GCTGCAGCTG GGGCCCGAGC CCCCCGCCCC GCACCCCGGG 
GTAAGCAAGC CCTGGAGATC TGGCGCCCCC CCGGAGGAGC CCGAGCGGCT GCCCCTCCAC 
GTGCGGTTCC TGGAGAACTG CCAGCCCCGG GCCGGCGGGG CGGGTGGAAG GGGGCCCCCC 
TCGGAGGACG GGCCGGGGGT CCCGCCGCCC AGCCCGCGCC GCTCTGTGCC GCCCTCGCCG 
ACCTCCCCGC GGGGCAGTGA AGAGAACGGG CTCCCCCTGC TGGTCCTGCC GCCGCCCGCC 
CCCTCGGTAG ACGTGGAAGA CGGCGAATTC CTCTTTGTGG AACCGCTGCC CCCGCCCCTC 
GAGTTTTCCA ACAGCTTCGA GAAGCCGGAG TCGCCTCTCA CGCCGGGGCC TCCCCACCCG 
CTGCCGGACC CACCCACCCC GACCACCCCA TTGCCCTCTG TGCCGCCCCC GGCTGTGGCC 
GCCGCCCCTC CCACCCTGGA CTCGACCGCA TCCAGCCTGA CTTCCTATGA CAGCGAGGTG 
GCAACGCTGA CCCAGGGGGC CCCCGCGGCT CCTGGGGACC CCCCTCCGCC AGGCCCTCCC 
GCCCCAGCCG CTCCGGCTCC CCCAGCCCCC CAGCCTGGCC CAGACCCTCC CCCGGGCACG 
GATTCTGGTA TTGAGGAGGT GGACAGTCGG AGCAGCAGCG ACCACCCCCT GGAGACCATC 
AGCAGCGCCT CCACGCTGAG CAGCCTGTCC GCTGAAGGCG GGGGCGGCGC AGGGGGTGGA 
ACTGGCGGCG TTGGAGGCAG TGGTGGCGCC AGCGTGGCCG GTGGACCAGA GCTCCTGGAT 
ACCTACGTGG CCTACTTGGA TGGCCAGGCC TTCGGGGGCA GCGGCACTCC TGGCCCCCCA 
TACCCCCCGC AGCTCATGAC TCCCTCTAAG CTCCGGGGCC GGGCGCTCGG GGCCGGCGGA 
GGCCTGCGGC TGGGCCCCGG TGGGGGACTC CGAGACCCTA TCACTCCCAC CAGCCCCACT 
GTCTCGGTGA CGGGGGCGGG AACCGATGGG CTGCTGGCCC TGAGCGGTTG TCCGGGATCC 
TCGACGGCAG GTGTGGCTGG CGGCCCCGTG GCTGTAGAGC CGGAAGGTCC GCCAGTGCCC 
TTGCCGTCGG CCTCCTCCCT CCCTCGAAAG CTGCTGCCCT GGGAGGAGGG CCCGGGCCCG 
CCGCCGCCCC CCCTGCCGGG GCCCTTAGCC CAACCTCAGG CCTCAGCCTT GGCCACTGTA 
AAAGCCAGCA TCATCAGTGA ACTCAGCTCC AAGCTTCAGC AGTTTGGGGG CTCCTCGGCA 
GCTGGTGGCG CTCTGCCCTG GGCCCGGGGA GGCAGCGGGG GAAGCGGGGA CAGCCACCAC 
GGGGGAGCCA GCTACGTCCC CGAGCGGACT TCCTCCCTGC AGCGGCAGAG ACTCTCCGAA 
GACTCCCAGT CCTCGCTCCT CTCCAAACCC GTGAGCAGCC TGTTTCAGAA CTGGTCCAAA 
CCACCTCTGC CACCACTCCC CACGGGAACC GCGGTGTCCC CTTCAGCCGC TGCAGCCCCG 
GGGGCCACCT CCCCATCGGC TTCCTCCTCC TCCACGTCCG CCCGCCACCT CCAGGGCGTG 
GAGTTCGAGA TGCGCCCACC TTTGCTCCGC CGGGCCCCCA GCCCCTCATT GCTGCCCGCC 
TCGGAGCACA AGGTCAGCCC TGCGCCCCGG CCCTCGTCCC TACCCATCCT GCCTTCCGGA 
CCCCTCTACC CGGGCCTCTT TGACATCCGA GGCTCCCCCA CCGGAGGGGC AGGAGGCTCG 
GCCGACCCCT TTGCCCCCGT CTTTGTACCG CCGCACCCCG GGATATCCGG GGGTCTCGGC 
GGGGCCTTGT CAGGGGCCTC CCGCTCCCTC TCACCGACCC GCCTGCTTTC CCTGCCCCCG 
GACAAGCCGT TCGGCGCTAA ACCTCTGGGC TTCTGGACCA AGTTCGATGT GGCTGACTGG 
CTAGAGTGGC TGGGCTTGGC CGAACACCGG GCCCGGTTCC TGGACCACGA GATCGATGGC 
TCCCACCTTC CCGCCTTGAC CAAGGAGGAC TACGTCGATT TGGGGGTGAC CAGGGTGGGA 
CACCGCATGA ACATCGACCG GGCTCTCAAG TTTTTCCTGG AGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002695260.2
CDS	242..6469
Translation	MTHSPASSEDEERHSVSECPEGGSESDSSPDGPGRGPRGTRGRGSAAPGSLASIRGLQSRSMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELLLYNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKETCARILLYRGANKDVKNNSGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARRRGPPGTGLTVPPALLRANSDTSMALPDWMVFAAPGTSSAGAPGPTAGPQGQAQPAAPSAKLSSGTLRSASSPRGARARSPSRGRHPEEAKRQPRGRPSSSGTPREGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFMAVKSYQAQAEGEISLSKGEKIKVLSIGEGGFWEGQVKGRIGWFPSDCLEEVANRSQEAKQESRSDKAKRLFRHYTVGSYDSFDAPSLMDGIGPGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKAPQQAKRLPPPAISLRSKSMTSELEEMEYEQQPPPMPSMEKKRTVYQMALNKLDEILAAAQQTISASESPGPGGLASLGKHRPKGFFTTESSFDPHHRSQPSYERPSYLPPGPGLMLRQKSIGAAEDDRPYLAPPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPAPSSSGRLTPSPRGGPFNPSSGGTHPASSPASFDGPAPPDTRIGSRDKSLYHSGPLPPPHHHPPHHHHHHAPPPQPHHHHAHPPHAPEMETGGSPDDPPPRLALGPQPSLRGWRGAAAPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPSQPEAAGGGVGGGGSSAPSPAPAVSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLSTDAGDEDGGDSSLGPGAPPGPRLRHSKSIDEGMFSAEPYLRLESAGAGSGYAGYAAGGRAYGGSSAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGPPQPPPRPPSPRYEAPPPTPHHHSPHAHHEPVLRLWGASPPDPARRELGYRAGLGSQEKSLPASPPAARRSLLHRLPPTAPGVGPLLLQLGPEPPAPHPGVSKPWRSGAPPEEPERLPLHVRFLENCQPRAGGAGGRGPPSEDGPGVPPPSPRRSVPPSPTSPRGSEENGLPLLVLPPPAPSVDVEDGEFLFVEPLPPPLEFSNSFEKPESPLTPGPPHPLPDPPTPTTPLPSVPPPAVAAAPPTLDSTASSLTSYDSEVATLTQGAPAAPGDPPPPGPPAPAAPAPPAPQPGPDPPPGTDSGIEEVDSRSSSDHPLETISSASTLSSLSAEGGGGAGGGTGGVGGSGGASVAGGPELLDTYVAYLDGQAFGGSGTPGPPYPPQLMTPSKLRGRALGAGGGLRLGPGGGLRDPITPTSPTVSVTGAGTDGLLALSGCPGSSTAGVAGGPVAVEPEGPPVPLPSASSLPRKLLPWEEGPGPPPPPLPGPLAQPQASALATVKASIISELSSKLQQFGGSSAAGGALPWARGGSGGSGDSHHGGASYVPERTSSLQRQRLSEDSQSSLLSKPVSSLFQNWSKPPLPPLPTGTAVSPSAAAAPGATSPSASSSSTSARHLQGVEFEMRPPLLRRAPSPSLLPASEHKVSPAPRPSSLPILPSGPLYPGLFDIRGSPTGGAGGSADPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKPLGFWTKFDVADWLEWLGLAEHRARFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLER

Target ORF information:

RefSeq Version	XM_002695214.4
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 1 (SHANK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002695214.4

ORF Insert Sequence:

ATGACCCACA GCCCCGCGTC AAGCGAGGAC GAAGAACGCC ACAGTGTCAG CGAGTGTCCC 
GAGGGGGGCT CAGAGTCCGA CAGCTCCCCA GACGGGCCCG GGAGAGGCCC CCGGGGGACC 
CGGGGCCGGG GCAGCGCAGC ACCTGGTAGC CTGGCCTCGA TCCGAGGCCT CCAGAGCCGC 
TCCATGTCCG TCCCAGACGA CGCCCACTTC AGCATGATGG TTTTTAGGAT CGGCATCCCT 
GACCTTCACC AGACAAAATG CCTGCGCTTC AACCCCGATG CCACCATCTG GACGGCCAAG 
CAGCAGGTGC TCTGTGCCCT GAGCGAGAGC CTCCAGGACG TGCTCAACTA TGGCCTGTTC 
CAGCCAGCCA CCTCGGGCCG GGACGCCAAC TTCCTGGAGG AGGAGCGGCT GCTGCGGGAG 
TACCCCCAGT CCTTCGAGAA GGGGGTGCCC TACCTGGAGT TCCGATACAA GACCCGTGTT 
TACAAGCAGA CCAACCTGGA TGAGAAGCAG CTGGCCAAGT TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TGGAGTACGT GCAGCTCGGG ACATCCGATA AGGTGGCACG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA TCATGACTCA GATTCAGGAG AGACCCCCCT GACGCTGGCT 
GCTCAGACCG AAGGCTCCGT AGAGGTGATT CGAACCCTAT GCCTGGGCGG GGCGCACATT 
GACTTCCGGG CCCGGGATGG CATGACCGCG CTACACAAGG CCGCCTGTGC CCGCCACTGC 
CTCGCTCTGA CGGCGCTCCT GGATCTTGGG GGCTCCCCCA ACTACAAGGA CCGCCGAGGG 
CTGACTCCCC TGTTCCACAC GGCTATGGTG GGGGGTGACC CCCGCTGTTG CGAGCTGCTC 
CTGTACAACA GGGCCCAGCT GGGCATAGCT GATGAGAACG GCTGGCAGGA GATTCACCAG 
GCCTGCCAGC GCGGTCACTC TCAGCATCTG GAACATCTGC TCTTCTATGG AGCTGAGCCT 
GGAGCCCAGA ACGCCTCGGG GAACACGGCC CTACACATCT GTGCCCTCTA CAATAAGGAG 
ACGTGTGCTA GGATCCTTCT CTATCGAGGG GCCAACAAGG ATGTCAAGAA TAACAGTGGA 
CAGACCCCCT TCCAGGTGGC AGTGATCGCT GGGAATTTTG AACTCGGGGA GCTGATCCGA 
AACCATCGAG AACAGGACGT GGTGCCCTTC CAGGAGTCCC CCAAATATGC AGCCCGGCGA 
CGGGGACCCC CGGGCACAGG GCTGACGGTG CCCCCCGCAC TGCTGCGGGC CAACAGTGAT 
ACCAGCATGG CCCTGCCCGA CTGGATGGTG TTCGCCGCCC CAGGGACCTC GTCTGCGGGG 
GCCCCCGGCC CCACCGCAGG GCCCCAGGGC CAGGCACAGC CCGCCGCCCC CAGCGCCAAG 
CTCAGCAGCG GGACCCTGCG AAGTGCCAGC AGCCCCCGGG GCGCCCGGGC CCGCTCCCCA 
TCCCGAGGGA GGCACCCTGA GGAGGCCAAG CGCCAGCCCC GTGGACGGCC CAGCTCCAGC 
GGGACACCCC GGGAAGGGCC AGCCGGGGGC ACGGGGGGCT CCGGGGGCCC CGGGGGCTCC 
CTGGGCAGCC GCGGGAGACG CAGGAAGCTC TACTCGGCAG TCCCTGGACG CTCCTTCATG 
GCTGTGAAGT CCTACCAGGC CCAAGCCGAG GGGGAGATCT CCCTGAGCAA GGGCGAAAAG 
ATCAAAGTGC TTAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGCATC 
GGCTGGTTCC CCTCTGACTG CCTGGAAGAG GTGGCCAACC GCTCCCAGGA AGCAAAGCAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCCGGCATT ATACCGTGGG CTCCTACGAC 
AGCTTTGATG CCCCAAGCTT GATGGATGGG ATTGGCCCAG GGAGCGATTA CATCATCAAA 
GAGAAGACAG TCCTGCTGCA GAAGAAGGAC AGTGAGGGGT TTGGGTTCGT GCTCCGGGGT 
GCCAAGGCGC AGACCCCCAT CGAGGAGTTC ACCCCCACCC CAGCCTTCCC GGCACTGCAG 
TATCTGGAGT CAGTGGACGA GGGCGGCGTG GCATGGCGGG CTGGACTGCG AATGGGAGAC 
TTCCTCATCG AGGTGAACGG GCAGAACGTG GTGAAGGTCG GCCACCGGCA GGTGGTGAAC 
ATGATCCGCC AAGGGGGCAA CACGCTGATG GTCAAGGTGG TGATGGTCAC CAGGCACCCG 
GACATGGATG AAGCTGTTCA CAAGAAAGCA CCCCAGCAAG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCT TGCGTTCCAA GTCTATGACC TCTGAGCTGG AGGAGATGGA GTACGAGCAG 
CAGCCGCCCC CGATGCCCAG CATGGAGAAA AAGAGGACTG TGTATCAGAT GGCTCTCAAC 
AAACTGGATG AAATCTTGGC GGCAGCTCAG CAGACTATCA GTGCAAGCGA GAGTCCTGGG 
CCTGGTGGCT TGGCGTCCCT GGGTAAACAC CGACCCAAAG GCTTCTTTAC CACTGAGTCG 
AGCTTTGATC CCCACCACCG CTCCCAGCCA AGTTACGAAC GTCCTTCCTA CCTGCCTCCT 
GGACCCGGGC TCATGCTCCG ACAAAAATCT ATTGGGGCTG CAGAGGACGA TAGACCCTAC 
CTAGCACCCC CAGCCATGAA GTTCAGCCGC AGTCTGTCTG TGCCTGGTTC GGAGGACATT 
CCCCCGCCGC CCACCACGTC CCCACCAGAG CCCCCCTACA GCACGCCTCC AGCCCCATCC 
TCCTCCGGCC GCCTCACTCC CTCTCCGCGG GGTGGGCCCT TCAACCCCAG CTCAGGTGGC 
ACCCACCCGG CCTCGTCCCC GGCGTCCTTC GATGGGCCTG CTCCCCCTGA CACCCGGATA 
GGGAGCCGAG ATAAGAGCCT GTATCACAGC GGGCCCCTGC CCCCACCCCA CCACCACCCG 
CCCCACCACC ACCACCACCA CGCCCCGCCC CCCCAGCCCC ACCACCACCA TGCCCACCCG 
CCTCACGCCC CGGAGATGGA GACTGGCGGC TCCCCCGACG ACCCCCCACC CCGCCTGGCT 
CTGGGGCCCC AGCCCAGCCT ACGAGGCTGG CGGGGCGCGG CCGCCCCGTC GGAAAAGAAC 
TCCATCCCCA TCCCCACCAT CATCATCAAG GCGCCGTCCA CCAGTAGCAG CGGCCGCAGC 
AGCCAGGGGA GCAGCACCGA GGCGGAGCCC CCGAGCCAGC CCGAGGCCGC GGGCGGCGGG 
GTCGGCGGCG GCGGCTCTTC CGCGCCCAGC CCCGCCCCGG CCGTGTCGCC CGTGCCGCCG 
TCCCCCTCGC CTGTGCCCAC CCCCGCCTCG CCCAGCGGCC CGGCCACCCT GGATTTCACC 
AGCCAGTTCG GAGCGGCCCT GGTGGGGGCG GCTCGGAGGG AAGGGGGCTG GCAGAACGAA 
GCCCGCCGGC GGTCCACGCT CTTCCTCTCC ACCGACGCGG GGGACGAGGA CGGCGGCGAC 
AGCTCGCTGG GCCCAGGGGC GCCCCCGGGC CCCCGGCTGC GCCACTCCAA GTCCATCGAC 
GAAGGCATGT TTTCGGCCGA GCCCTACCTC CGGCTGGAGT CGGCGGGCGC CGGCAGCGGC 
TACGCGGGCT ACGCGGCCGG CGGCCGAGCC TACGGGGGCA GCAGCGCCTT CACCAGCTTC 
CTGCCGCCGC GACCCCTGGT CCACCCGCTG ACCGGCAAGG CCTTGGACCC GGCCTCCCCA 
CTCGGGCTGG CCCTGGCCGC CCGGGAGCGG GCGCTGAAGG AGTCCTCGGA GGGCGGTGGG 
CCCCCCCAGC CCCCTCCAAG GCCCCCATCG CCCCGCTACG AGGCCCCGCC GCCCACCCCG 
CACCACCACT CGCCCCACGC CCACCACGAG CCAGTGCTGC GCCTCTGGGG AGCCTCGCCC 
CCGGACCCGG CCCGCCGGGA GCTGGGGTAC CGGGCAGGGC TGGGCAGCCA GGAGAAGTCC 
CTTCCGGCCA GCCCACCGGC GGCCCGACGC TCCTTGCTGC ACCGCCTGCC TCCCACAGCT 
CCCGGGGTCG GGCCCCTCCT GCTGCAGCTG GGGCCCGAGC CCCCCGCCCC GCACCCCGGG 
GTAAGCAAGC CCTGGAGATC TGGCGCCCCC CCGGAGGAGC CCGAGCGGCT GCCCCTCCAC 
GTGCGGTTCC TGGAGAACTG CCAGCCCCGG GCCGGCGGGG CGGGTGGAAG GGGGCCCCCC 
TCGGAGGACG GGCCGGGGGT CCCGCCGCCC AGCCCGCGCC GCTCTGTGCC GCCCTCGCCG 
ACCTCCCCGC GGGGCAGTGA AGAGAACGGG CTCCCCCTGC TGGTCCTGCC GCCGCCCGCC 
CCCTCGGTAG ACGTGGAAGA CGGCGAATTC CTCTTTGTGG AACCGCTGCC CCCGCCCCTC 
GAGTTTTCCA ACAGCTTCGA GAAGCCGGAG TCGCCTCTCA CGCCGGGGCC TCCCCACCCG 
CTGCCGGACC CACCCACCCC GACCACCCCA TTGCCCTCTG TGCCGCCCCC GGCTGTGGCC 
GCCGCCCCTC CCACCCTGGA CTCGACCGCA TCCAGCCTGA CTTCCTATGA CAGCGAGGTG 
GCAACGCTGA CCCAGGGGGC CCCCGCGGCT CCTGGGGACC CCCCTCCGCC AGGCCCTCCC 
GCCCCAGCCG CTCCGGCTCC CCCAGCCCCC CAGCCTGGCC CAGACCCTCC CCCGGGCACG 
GATTCTGGTA TTGAGGAGGT GGACAGTCGG AGCAGCAGCG ACCACCCCCT GGAGACCATC 
AGCAGCGCCT CCACGCTGAG CAGCCTGTCC GCTGAAGGCG GGGGCGGCGC AGGGGGTGGA 
ACTGGCGGCG TTGGAGGCAG TGGTGGCGCC AGCGTGGCCG GTGGACCAGA GCTCCTGGAT 
ACCTACGTGG CCTACTTGGA TGGCCAGGCC TTCGGGGGCA GCGGCACTCC TGGCCCCCCA 
TACCCCCCGC AGCTCATGAC TCCCTCTAAG CTCCGGGGCC GGGCGCTCGG GGCCGGCGGA 
GGCCTGCGGC TGGGCCCCGG TGGGGGACTC CGAGACCCTA TCACTCCCAC CAGCCCCACT 
GTCTCGGTGA CGGGGGCGGG AACCGATGGG CTGCTGGCCC TGAGCGGTTG TCCGGGATCC 
TCGACGGCAG GTGTGGCTGG CGGCCCCGTG GCTGTAGAGC CGGAAGGTCC GCCAGTGCCC 
TTGCCGTCGG CCTCCTCCCT CCCTCGAAAG CTGCTGCCCT GGGAGGAGGG CCCGGGCCCG 
CCGCCGCCCC CCCTGCCGGG GCCCTTAGCC CAACCTCAGG CCTCAGCCTT GGCCACTGTA 
AAAGCCAGCA TCATCAGTGA ACTCAGCTCC AAGCTTCAGC AGTTTGGGGG CTCCTCGGCA 
GCTGGTGGCG CTCTGCCCTG GGCCCGGGGA GGCAGCGGGG GAAGCGGGGA CAGCCACCAC 
GGGGGAGCCA GCTACGTCCC CGAGCGGACT TCCTCCCTGC AGCGGCAGAG ACTCTCCGAA 
GACTCCCAGT CCTCGCTCCT CTCCAAACCC GTGAGCAGCC TGTTTCAGAA CTGGTCCAAA 
CCACCTCTGC CACCACTCCC CACGGGAACC GCGGTGTCCC CTTCAGCCGC TGCAGCCCCG 
GGGGCCACCT CCCCATCGGC TTCCTCCTCC TCCACGTCCG CCCGCCACCT CCAGGGCGTG 
GAGTTCGAGA TGCGCCCACC TTTGCTCCGC CGGGCCCCCA GCCCCTCATT GCTGCCCGCC 
TCGGAGCACA AGGTCAGCCC TGCGCCCCGG CCCTCGTCCC TACCCATCCT GCCTTCCGGA 
CCCCTCTACC CGGGCCTCTT TGACATCCGA GGCTCCCCCA CCGGAGGGGC AGGAGGCTCG 
GCCGACCCCT TTGCCCCCGT CTTTGTACCG CCGCACCCCG GGATATCCGG GGGTCTCGGC 
GGGGCCTTGT CAGGGGCCTC CCGCTCCCTC TCACCGACCC GCCTGCTTTC CCTGCCCCCG 
GACAAGCCGT TCGGCGCTAA ACCTCTGGGC TTCTGGACCA AGTTCGATGT GGCTGACTGG 
CTAGAGTGGC TGGGCTTGGC CGAACACCGG GCCCGGTTCC TGGACCACGA GATCGATGGC 
TCCCACCTTC CCGCCTTGAC CAAGGAGGAC TACGTCGATT TGGGGGTGAC CAGGGTGGGA 
CACCGCATGA ACATCGACCG GGCTCTCAAG TTTTTCCTGG AGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK1 ( XM_015467539.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323025.1 Bos taurus SH3 and multiple ankyrin repeat domains 1 (SHANK1), transcript variant X2, mRNA.

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CloneID	OBa94991
Clone ID Related Accession (Same CDS sequence)	XM_015467539.1
Accession Version	XM_015467539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: SH3 and multiple ankyrin repeat domains protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGACCCACA GCCCCGCGTC AAGCGAGGAC GAAGAACGCC ACAGTGTCAG CGAGTGTCCC 
GAGGGGGGCT CAGAGTCCGA CAGCTCCCCA GACGGGCCCG GGAGAGGCCC CCGGGGGACC 
CGGGGCCGGG GCAGCGCAGC ACCTGGTAGC CTGGCCTCGA TCCGAGGCCT CCAGAGCCGC 
TCCATGTCCG TCCCAGACGA CGCCCACTTC AGCATGATGG TTTTTAGGAT CGGCATCCCT 
GACCTTCACC AGACAAAATG CCTGCGCTTC AACCCCGATG CCACCATCTG GACGGCCAAG 
CAGCAGGTGC TCTGTGCCCT GAGCGAGAGC CTCCAGGACG TGCTCAACTA TGGCCTGTTC 
CAGCCAGCCA CCTCGGGCCG GGACGCCAAC TTCCTGGAGG AGGAGCGGCT GCTGCGGGAG 
TACCCCCAGT CCTTCGAGAA GGGGGTGCCC TACCTGGAGT TCCGATACAA GACCCGTGTT 
TACAAGCAGA CCAACCTGGA TGAGAAGCAG CTGGCCAAGT TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TGGAGTACGT GCAGCTCGGG ACATCCGATA AGGTGGCACG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA TCATGACTCA GATTCAGGAG AGACCCCCCT GACGCTGGCT 
GCTCAGACCG AAGGCTCCGT AGAGGTGATT CGAACCCTAT GCCTGGGCGG GGCGCACATT 
GACTTCCGGG CCCGGGATGG CATGACCGCG CTACACAAGG CCGCCTGTGC CCGCCACTGC 
CTCGCTCTGA CGGCGCTCCT GGATCTTGGG GGCTCCCCCA ACTACAAGGA CCGCCGAGGG 
CTGACTCCCC TGTTCCACAC GGCTATGGTG GGGGGTGACC CCCGCTGTTG CGAGCTGCTC 
CTGTACAACA GGGCCCAGCT GGGCATAGCT GATGAGAACG GCTGGCAGGA GATTCACCAG 
GCCTGCCAGC GCGGTCACTC TCAGCATCTG GAACATCTGC TCTTCTATGG AGCTGAGCCT 
GGAGCCCAGA ACGCCTCGGG GAACACGGCC CTACACATCT GTGCCCTCTA CAATAAGGAG 
ACGTGTGCTA GGATCCTTCT CTATCGAGGG GCCAACAAGG ATGTCAAGAA TAACAGTGGA 
CAGACCCCCT TCCAGGTGGC AGTGATCGCT GGGAATTTTG AACTCGGGGA GCTGATCCGA 
AACCATCGAG AACAGGACGT GGTGCCCTTC CAGGAGTCCC CCAAATATGC AGCCCGGCGA 
CGGGGACCCC CGGGCACAGG GCTGACGGTG CCCCCCGCAC TGCTGCGGGC CAACAGTGAT 
ACCAGCATGG CCCTGCCCGA CTGGATGGTG TTCGCCGCCC CAGGGACCTC GTCTGCGGGG 
GCCCCCGGCC CCACCGCAGG GCCCCAGGGC CAGGCACAGC CCGCCGCCCC CAGCGCCAAG 
CTCAGCAGCG GGACCCTGCG AAGTGCCAGC AGCCCCCGGG GCGCCCGGGC CCGCTCCCCA 
TCCCGAGGGA GGCACCCTGA GGAGGCCAAG CGCCAGCCCC GTGGACGGCC CAGCTCCAGC 
GGGACACCCC GGGAAGGGCC AGCCGGGGGC ACGGGGGGCT CCGGGGGCCC CGGGGGCTCC 
CTGGGCAGCC GCGGGAGACG CAGGAAGCTC TACTCGGCAG TCCCTGGACG CTCCTTCATG 
GCTGTGAAGT CCTACCAGGC CCAAGCCGAG GGGGAGATCT CCCTGAGCAA GGGCGAAAAG 
ATCAAAGTGC TTAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGCATC 
GGCTGGTTCC CCTCTGACTG CCTGGAAGAG GTGGCCAACC GCTCCCAGGA AGCAAAGCAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCCGGCATT ATACCGTGGG CTCCTACGAC 
AGCTTTGATG CCCCAAGCTT GATGGATGGG ATTGGCCCAG GGAGCGATTA CATCATCAAA 
GAGAAGACAG TCCTGCTGCA GAAGAAGGAC AGTGAGGGGT TTGGGTTCGT GCTCCGGGGT 
GCCAAGGCGC AGACCCCCAT CGAGGAGTTC ACCCCCACCC CAGCCTTCCC GGCACTGCAG 
TATCTGGAGT CAGTGGACGA GGGCGGCGTG GCATGGCGGG CTGGACTGCG AATGGGAGAC 
TTCCTCATCG AGGTGAACGG GCAGAACGTG GTGAAGGTCG GCCACCGGCA GGTGGTGAAC 
ATGATCCGCC AAGGGGGCAA CACGCTGATG GTCAAGGTGG TGATGGTCAC CAGGCACCCG 
GACATGGATG AAGCTGTTCA CAAGAAAGCA CCCCAGCAAG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCT TGCGTTCCAA GTCTATGACC TCTGAGCTGG AGGAGATGGA GTACGAGCAG 
CAGCCGCCCC CGATGCCCAG CATGGAGAAA AAGAGGACTG TGTATCAGAT GGCTCTCAAC 
AAACTGGATG AAATCTTGGC GGCAGCTCAG CAGACTATCA GTGCAAGCGA GAGTCCTGGG 
CCTGGTGGCT TGGCGTCCCT GGGTAAACAC CGACCCAAAG GCTTCTTTAC CACTGAGTCG 
AGCTTTGATC CCCACCACCG CTCCCAGCCA AGTTACGAAC GTCCTTCCTA CCTGCCTCCT 
GGACCCGGGC TCATGCTCCG ACAAAAATCT ATTGGGGCTG CAGAGGACGA TAGACCCTAC 
CTAGCACCCC CAGCCATGAA GTTCAGCCGC AGTCTGTCTG TGCCTGGTTC GGAGGACATT 
CCCCCGCCGC CCACCACGTC CCCACCAGAG CCCCCCTACA GCACGCCTCC AGCCCCATCC 
TCCTCCGGCC GCCTCACTCC CTCTCCGCGG GGTGGGCCCT TCAACCCCAG CTCAGGTGGC 
ACCCACCCGG CCTCGTCCCC GGCGTCCTTC GATGGGCCTG CTCCCCCTGA CACCCGGATA 
GGGAGCCGAG ATAAGAGCCT GTATCACAGC GGGCCCCTGC CCCCACCCCA CCACCACCCG 
CCCCACCACC ACCACCACCA CGCCCCGCCC CCCCAGCCCC ACCACCACCA TGCCCACCCG 
CCTCACGCCC CGGAGATGGA GACTGGCGGC TCCCCCGACG ACCCCCCACC CCGCCTGGCT 
CTGGGGCCCC AGCCCAGCCT ACGAGGCTGG CGGGGCGGCG GGCGGGCCCA GCCCGACCCC 
GGGTGCTCTG TCCCGTCGCA CCACGGCAGC GGGGGCGGCG CGGGCGGCCC CTCCCAGGCT 
CCGGCGGCTG CGCTACTTCA GCTGCCTCCG CGGGCGGCCA GCGCGCGCCA TGTATGTGCC 
CGCCCGTTCG GCCGCGGGCG CAAGGGCCCG CTGGTCAACA AGCCAAAAGT CGTCGAAGGC 
GAGCCCAGAA GAGGCGGCGC CNNTCCGCCT GCCCCCTCGC CACGTCTCCG GCGTTCCGCA 
GCCGCCCCGC AGCCGCCATG CGCCGCGGCC GCCCCGTCGG AAAAGAACTC CATCCCCATC 
CCCACCATCA TCATCAAGGC GCCGTCCACC AGTAGCAGCG GCCGCAGCAG CCAGGGGAGC 
AGCACCGAGG CGGAGCCCCC GAGCCAGCCC GAGGCCGCGG GCGGCGGGGT CGGCGGCGGC 
GGCTCTTCCG CGCCCAGCCC CGCCCCGGCC GTGTCGCCCG TGCCGCCGTC CCCCTCGCCT 
GTGCCCACCC CCGCCTCGCC CAGCGGCCCG GCCACCCTGG ATTTCACCAG CCAGTTCGGA 
GCGGCCCTGG TGGGGGCGGC TCGGAGGGAA GGGGGCTGGC AGAACGAAGC CCGCCGGCGG 
TCCACGCTCT TCCTCTCCAC CGACGCGGGG GACGAGGACG GCGGCGACAG CTCGCTGGGC 
CCAGGGGCGC CCCCGGGCCC CCGGCTGCGC CACTCCAAGT CCATCGACGA AGGCATGTTT 
TCGGCCGAGC CCTACCTCCG GCTGGAGTCG GCGGGCGCCG GCAGCGGCTA CGCGGGCTAC 
GCGGCCGGCG GCCGAGCCTA CGGGGGCAGC AGCGCCTTCA CCAGCTTCCT GCCGCCGCGA 
CCCCTGGTCC ACCCGCTGAC CGGCAAGGCC TTGGACCCGG CCTCCCCACT CGGGCTGGCC 
CTGGCCGCCC GGGAGCGGGC GCTGAAGGAG TCCTCGGAGG GCGGTGGGCC CCCCCAGCCC 
CCTCCAAGGC CCCCATCGCC CCGCTACGAG GCCCCGCCGC CCACCCCGCA CCACCACTCG 
CCCCACGCCC ACCACGAGCC AGTGCTGCGC CTCTGGGGAG CCTCGCCCCC GGACCCGGCC 
CGCCGGGAGC TGGGGTACCG GGCAGGGCTG GGCAGCCAGG AGAAGTCCCT TCCGGCCAGC 
CCACCGGCGG CCCGACGCTC CTTGCTGCAC CGCCTGCCTC CCACAGCTCC CGGGGTCGGG 
CCCCTCCTGC TGCAGCTGGG GCCCGAGCCC CCCGCCCCGC ACCCCGGGGT AAGCAAGCCC 
TGGAGATCTG GCGCCCCCCC GGAGGAGCCC GAGCGGCTGC CCCTCCACGT GCGGTTCCTG 
GAGAACTGCC AGCCCCGGGC CGGCGGGGCG GGTGGAAGGG GGCCCCCCTC GGAGGACGGG 
CCGGGGGTCC CGCCGCCCAG CCCGCGCCGC TCTGTGCCGC CCTCGCCGAC CTCCCCGCGG 
GGCAGTGAAG AGAACGGGCT CCCCCTGCTG GTCCTGCCGC CGCCCGCCCC CTCGGTAGAC 
GTGGAAGACG GCGAATTCCT CTTTGTGGAA CCGCTGCCCC CGCCCCTCGA GTTTTCCAAC 
AGCTTCGAGA AGCCGGAGTC GCCTCTCACG CCGGGGCCTC CCCACCCGCT GCCGGACCCA 
CCCACCCCGA CCACCCCATT GCCCTCTGTG CCGCCCCCGG CTGTGGCCGC CGCCCCTCCC 
ACCCTGGACT CGACCGCATC CAGCCTGACT TCCTATGACA GCGAGGTGGC AACGCTGACC 
CAGGGGGCCC CCGCGGCTCC TGGGGACCCC CCTCCGCCAG GCCCTCCCGC CCCAGCCGCT 
CCGGCTCCCC CAGCCCCCCA GCCTGGCCCA GACCCTCCCC CGGGCACGGA TTCTGGTATT 
GAGGAGGTGG ACAGTCGGAG CAGCAGCGAC CACCCCCTGG AGACCATCAG CAGCGCCTCC 
ACGCTGAGCA GCCTGTCCGC TGAAGGCGGG GGCGGCGCAG GGGGTGGAAC TGGCGGCGTT 
GGAGGCAGTG GTGGCGCCAG CGTGGCCGGT GGACCAGAGC TCCTGGATAC CTACGTGGCC 
TACTTGGATG GCCAGGCCTT CGGGGGCAGC GGCACTCCTG GCCCCCCATA CCCCCCGCAG 
CTCATGACTC CCTCTAAGCT CCGGGGCCGG GCGCTCGGGG CCGGCGGAGG CCTGCGGCTG 
GGCCCCGGTG GGGGACTCCG AGACCCTATC ACTCCCACCA GCCCCACTGT CTCGGTGACG 
GGGGCGGGAA CCGATGGGCT GCTGGCCCTG AGCGGTTGTC CGGGATCCTC GACGGCAGGT 
GTGGCTGGCG GCCCCGTGGC TGTAGAGCCG GAAGGTCCGC CAGTGCCCTT GCCGTCGGCC 
TCCTCCCTCC CTCGAAAGCT GCTGCCCTGG GAGGAGGGCC CGGGCCCGCC GCCGCCCCCC 
CTGCCGGGGC CCTTAGCCCA ACCTCAGGCC TCAGCCTTGG CCACTGTAAA AGCCAGCATC 
ATCAGTGAAC TCAGCTCCAA GCTTCAGCAG TTTGGGGGCT CCTCGGCAGC TGGTGGCGCT 
CTGCCCTGGG CCCGGGGAGG CAGCGGGGGA AGCGGGGACA GCCACCACGG GGGAGCCAGC 
TACGTCCCCG AGCGGACTTC CTCCCTGCAG CGGCAGAGAC TCTCCGAAGA CTCCCAGTCC 
TCGCTCCTCT CCAAACCCGT GAGCAGCCTG TTTCAGAACT GGTCCAAACC ACCTCTGCCA 
CCACTCCCCA CGGGAACCGC GGTGTCCCCT TCAGCCGCTG CAGCCCCGGG GGCCACCTCC 
CCATCGGCTT CCTCCTCCTC CACGTCCGCC CGCCACCTCC AGGGCGTGGA GTTCGAGATG 
CGCCCACCTT TGCTCCGCCG GGCCCCCAGC CCCTCATTGC TGCCCGCCTC GGAGCACAAG 
GTCAGCCCTG CGCCCCGGCC CTCGTCCCTA CCCATCCTGC CTTCCGGACC CCTCTACCCG 
GGCCTCTTTG ACATCCGAGG CTCCCCCACC GGAGGGGCAG GAGGCTCGGC CGACCCCTTT 
GCCCCCGTCT TTGTACCGCC GCACCCCGGG ATATCCGGGG GTCTCGGCGG GGCCTTGTCA 
GGGGCCTCCC GCTCCCTCTC ACCGACCCGC CTGCTTTCCC TGCCCCCGGA CAAGCCGTTC 
GGCGCTAAAC CTCTGGGCTT CTGGACCAAG TTCGATGTGG CTGACTGGCT AGAGTGGCTG 
GGCTTGGCCG AACACCGGGC CCGGTTCCTG GACCACGAGA TCGATGGCTC CCACCTTCCC 
GCCTTGACCA AGGAGGACTA CGTCGATTTG GGGGTGACCA GGGTGGGACA CCGCATGAAC 
ATCGACCGGG CTCTCAAGTT TTTCCTGGAG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323025.1
CDS	243..6758
Translation	MTHSPASSEDEERHSVSECPEGGSESDSSPDGPGRGPRGTRGRGSAAPGSLASIRGLQSRSMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELLLYNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKETCARILLYRGANKDVKNNSGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARRRGPPGTGLTVPPALLRANSDTSMALPDWMVFAAPGTSSAGAPGPTAGPQGQAQPAAPSAKLSSGTLRSASSPRGARARSPSRGRHPEEAKRQPRGRPSSSGTPREGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFMAVKSYQAQAEGEISLSKGEKIKVLSIGEGGFWEGQVKGRIGWFPSDCLEEVANRSQEAKQESRSDKAKRLFRHYTVGSYDSFDAPSLMDGIGPGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKAPQQAKRLPPPAISLRSKSMTSELEEMEYEQQPPPMPSMEKKRTVYQMALNKLDEILAAAQQTISASESPGPGGLASLGKHRPKGFFTTESSFDPHHRSQPSYERPSYLPPGPGLMLRQKSIGAAEDDRPYLAPPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPAPSSSGRLTPSPRGGPFNPSSGGTHPASSPASFDGPAPPDTRIGSRDKSLYHSGPLPPPHHHPPHHHHHHAPPPQPHHHHAHPPHAPEMETGGSPDDPPPRLALGPQPSLRGWRGGGRAQPDPGCSVPSHHGSGGGAGGPSQAPAAALLQLPPRAASARHVCARPFGRGRKGPLVNKPKVVEGEPRRGGAnullPPAPSPRLRRSAAAPQPPCAAAAPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPSQPEAAGGGVGGGGSSAPSPAPAVSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLSTDAGDEDGGDSSLGPGAPPGPRLRHSKSIDEGMFSAEPYLRLESAGAGSGYAGYAAGGRAYGGSSAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGPPQPPPRPPSPRYEAPPPTPHHHSPHAHHEPVLRLWGASPPDPARRELGYRAGLGSQEKSLPASPPAARRSLLHRLPPTAPGVGPLLLQLGPEPPAPHPGVSKPWRSGAPPEEPERLPLHVRFLENCQPRAGGAGGRGPPSEDGPGVPPPSPRRSVPPSPTSPRGSEENGLPLLVLPPPAPSVDVEDGEFLFVEPLPPPLEFSNSFEKPESPLTPGPPHPLPDPPTPTTPLPSVPPPAVAAAPPTLDSTASSLTSYDSEVATLTQGAPAAPGDPPPPGPPAPAAPAPPAPQPGPDPPPGTDSGIEEVDSRSSSDHPLETISSASTLSSLSAEGGGGAGGGTGGVGGSGGASVAGGPELLDTYVAYLDGQAFGGSGTPGPPYPPQLMTPSKLRGRALGAGGGLRLGPGGGLRDPITPTSPTVSVTGAGTDGLLALSGCPGSSTAGVAGGPVAVEPEGPPVPLPSASSLPRKLLPWEEGPGPPPPPLPGPLAQPQASALATVKASIISELSSKLQQFGGSSAAGGALPWARGGSGGSGDSHHGGASYVPERTSSLQRQRLSEDSQSSLLSKPVSSLFQNWSKPPLPPLPTGTAVSPSAAAAPGATSPSASSSSTSARHLQGVEFEMRPPLLRRAPSPSLLPASEHKVSPAPRPSSLPILPSGPLYPGLFDIRGSPTGGAGGSADPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKPLGFWTKFDVADWLEWLGLAEHRARFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLER

Target ORF information:

RefSeq Version	XM_015467539.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 1 (SHANK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015467539.1

ORF Insert Sequence:

ATGACCCACA GCCCCGCGTC AAGCGAGGAC GAAGAACGCC ACAGTGTCAG CGAGTGTCCC 
GAGGGGGGCT CAGAGTCCGA CAGCTCCCCA GACGGGCCCG GGAGAGGCCC CCGGGGGACC 
CGGGGCCGGG GCAGCGCAGC ACCTGGTAGC CTGGCCTCGA TCCGAGGCCT CCAGAGCCGC 
TCCATGTCCG TCCCAGACGA CGCCCACTTC AGCATGATGG TTTTTAGGAT CGGCATCCCT 
GACCTTCACC AGACAAAATG CCTGCGCTTC AACCCCGATG CCACCATCTG GACGGCCAAG 
CAGCAGGTGC TCTGTGCCCT GAGCGAGAGC CTCCAGGACG TGCTCAACTA TGGCCTGTTC 
CAGCCAGCCA CCTCGGGCCG GGACGCCAAC TTCCTGGAGG AGGAGCGGCT GCTGCGGGAG 
TACCCCCAGT CCTTCGAGAA GGGGGTGCCC TACCTGGAGT TCCGATACAA GACCCGTGTT 
TACAAGCAGA CCAACCTGGA TGAGAAGCAG CTGGCCAAGT TGCACACGAA GACGGGGTTG 
AAGAAGTTCC TGGAGTACGT GCAGCTCGGG ACATCCGATA AGGTGGCACG GCTGCTGGAC 
AAGGGGCTGG ACCCCAATTA TCATGACTCA GATTCAGGAG AGACCCCCCT GACGCTGGCT 
GCTCAGACCG AAGGCTCCGT AGAGGTGATT CGAACCCTAT GCCTGGGCGG GGCGCACATT 
GACTTCCGGG CCCGGGATGG CATGACCGCG CTACACAAGG CCGCCTGTGC CCGCCACTGC 
CTCGCTCTGA CGGCGCTCCT GGATCTTGGG GGCTCCCCCA ACTACAAGGA CCGCCGAGGG 
CTGACTCCCC TGTTCCACAC GGCTATGGTG GGGGGTGACC CCCGCTGTTG CGAGCTGCTC 
CTGTACAACA GGGCCCAGCT GGGCATAGCT GATGAGAACG GCTGGCAGGA GATTCACCAG 
GCCTGCCAGC GCGGTCACTC TCAGCATCTG GAACATCTGC TCTTCTATGG AGCTGAGCCT 
GGAGCCCAGA ACGCCTCGGG GAACACGGCC CTACACATCT GTGCCCTCTA CAATAAGGAG 
ACGTGTGCTA GGATCCTTCT CTATCGAGGG GCCAACAAGG ATGTCAAGAA TAACAGTGGA 
CAGACCCCCT TCCAGGTGGC AGTGATCGCT GGGAATTTTG AACTCGGGGA GCTGATCCGA 
AACCATCGAG AACAGGACGT GGTGCCCTTC CAGGAGTCCC CCAAATATGC AGCCCGGCGA 
CGGGGACCCC CGGGCACAGG GCTGACGGTG CCCCCCGCAC TGCTGCGGGC CAACAGTGAT 
ACCAGCATGG CCCTGCCCGA CTGGATGGTG TTCGCCGCCC CAGGGACCTC GTCTGCGGGG 
GCCCCCGGCC CCACCGCAGG GCCCCAGGGC CAGGCACAGC CCGCCGCCCC CAGCGCCAAG 
CTCAGCAGCG GGACCCTGCG AAGTGCCAGC AGCCCCCGGG GCGCCCGGGC CCGCTCCCCA 
TCCCGAGGGA GGCACCCTGA GGAGGCCAAG CGCCAGCCCC GTGGACGGCC CAGCTCCAGC 
GGGACACCCC GGGAAGGGCC AGCCGGGGGC ACGGGGGGCT CCGGGGGCCC CGGGGGCTCC 
CTGGGCAGCC GCGGGAGACG CAGGAAGCTC TACTCGGCAG TCCCTGGACG CTCCTTCATG 
GCTGTGAAGT CCTACCAGGC CCAAGCCGAG GGGGAGATCT CCCTGAGCAA GGGCGAAAAG 
ATCAAAGTGC TTAGCATCGG GGAAGGAGGC TTCTGGGAAG GCCAGGTCAA AGGTCGCATC 
GGCTGGTTCC CCTCTGACTG CCTGGAAGAG GTGGCCAACC GCTCCCAGGA AGCAAAGCAA 
GAAAGCCGAA GTGACAAGGC AAAGAGACTC TTCCGGCATT ATACCGTGGG CTCCTACGAC 
AGCTTTGATG CCCCAAGCTT GATGGATGGG ATTGGCCCAG GGAGCGATTA CATCATCAAA 
GAGAAGACAG TCCTGCTGCA GAAGAAGGAC AGTGAGGGGT TTGGGTTCGT GCTCCGGGGT 
GCCAAGGCGC AGACCCCCAT CGAGGAGTTC ACCCCCACCC CAGCCTTCCC GGCACTGCAG 
TATCTGGAGT CAGTGGACGA GGGCGGCGTG GCATGGCGGG CTGGACTGCG AATGGGAGAC 
TTCCTCATCG AGGTGAACGG GCAGAACGTG GTGAAGGTCG GCCACCGGCA GGTGGTGAAC 
ATGATCCGCC AAGGGGGCAA CACGCTGATG GTCAAGGTGG TGATGGTCAC CAGGCACCCG 
GACATGGATG AAGCTGTTCA CAAGAAAGCA CCCCAGCAAG CTAAGCGGCT CCCGCCCCCA 
GCCATCTCCT TGCGTTCCAA GTCTATGACC TCTGAGCTGG AGGAGATGGA GTACGAGCAG 
CAGCCGCCCC CGATGCCCAG CATGGAGAAA AAGAGGACTG TGTATCAGAT GGCTCTCAAC 
AAACTGGATG AAATCTTGGC GGCAGCTCAG CAGACTATCA GTGCAAGCGA GAGTCCTGGG 
CCTGGTGGCT TGGCGTCCCT GGGTAAACAC CGACCCAAAG GCTTCTTTAC CACTGAGTCG 
AGCTTTGATC CCCACCACCG CTCCCAGCCA AGTTACGAAC GTCCTTCCTA CCTGCCTCCT 
GGACCCGGGC TCATGCTCCG ACAAAAATCT ATTGGGGCTG CAGAGGACGA TAGACCCTAC 
CTAGCACCCC CAGCCATGAA GTTCAGCCGC AGTCTGTCTG TGCCTGGTTC GGAGGACATT 
CCCCCGCCGC CCACCACGTC CCCACCAGAG CCCCCCTACA GCACGCCTCC AGCCCCATCC 
TCCTCCGGCC GCCTCACTCC CTCTCCGCGG GGTGGGCCCT TCAACCCCAG CTCAGGTGGC 
ACCCACCCGG CCTCGTCCCC GGCGTCCTTC GATGGGCCTG CTCCCCCTGA CACCCGGATA 
GGGAGCCGAG ATAAGAGCCT GTATCACAGC GGGCCCCTGC CCCCACCCCA CCACCACCCG 
CCCCACCACC ACCACCACCA CGCCCCGCCC CCCCAGCCCC ACCACCACCA TGCCCACCCG 
CCTCACGCCC CGGAGATGGA GACTGGCGGC TCCCCCGACG ACCCCCCACC CCGCCTGGCT 
CTGGGGCCCC AGCCCAGCCT ACGAGGCTGG CGGGGCGGCG GGCGGGCCCA GCCCGACCCC 
GGGTGCTCTG TCCCGTCGCA CCACGGCAGC GGGGGCGGCG CGGGCGGCCC CTCCCAGGCT 
CCGGCGGCTG CGCTACTTCA GCTGCCTCCG CGGGCGGCCA GCGCGCGCCA TGTATGTGCC 
CGCCCGTTCG GCCGCGGGCG CAAGGGCCCG CTGGTCAACA AGCCAAAAGT CGTCGAAGGC 
GAGCCCAGAA GAGGCGGCGC CNNTCCGCCT GCCCCCTCGC CACGTCTCCG GCGTTCCGCA 
GCCGCCCCGC AGCCGCCATG CGCCGCGGCC GCCCCGTCGG AAAAGAACTC CATCCCCATC 
CCCACCATCA TCATCAAGGC GCCGTCCACC AGTAGCAGCG GCCGCAGCAG CCAGGGGAGC 
AGCACCGAGG CGGAGCCCCC GAGCCAGCCC GAGGCCGCGG GCGGCGGGGT CGGCGGCGGC 
GGCTCTTCCG CGCCCAGCCC CGCCCCGGCC GTGTCGCCCG TGCCGCCGTC CCCCTCGCCT 
GTGCCCACCC CCGCCTCGCC CAGCGGCCCG GCCACCCTGG ATTTCACCAG CCAGTTCGGA 
GCGGCCCTGG TGGGGGCGGC TCGGAGGGAA GGGGGCTGGC AGAACGAAGC CCGCCGGCGG 
TCCACGCTCT TCCTCTCCAC CGACGCGGGG GACGAGGACG GCGGCGACAG CTCGCTGGGC 
CCAGGGGCGC CCCCGGGCCC CCGGCTGCGC CACTCCAAGT CCATCGACGA AGGCATGTTT 
TCGGCCGAGC CCTACCTCCG GCTGGAGTCG GCGGGCGCCG GCAGCGGCTA CGCGGGCTAC 
GCGGCCGGCG GCCGAGCCTA CGGGGGCAGC AGCGCCTTCA CCAGCTTCCT GCCGCCGCGA 
CCCCTGGTCC ACCCGCTGAC CGGCAAGGCC TTGGACCCGG CCTCCCCACT CGGGCTGGCC 
CTGGCCGCCC GGGAGCGGGC GCTGAAGGAG TCCTCGGAGG GCGGTGGGCC CCCCCAGCCC 
CCTCCAAGGC CCCCATCGCC CCGCTACGAG GCCCCGCCGC CCACCCCGCA CCACCACTCG 
CCCCACGCCC ACCACGAGCC AGTGCTGCGC CTCTGGGGAG CCTCGCCCCC GGACCCGGCC 
CGCCGGGAGC TGGGGTACCG GGCAGGGCTG GGCAGCCAGG AGAAGTCCCT TCCGGCCAGC 
CCACCGGCGG CCCGACGCTC CTTGCTGCAC CGCCTGCCTC CCACAGCTCC CGGGGTCGGG 
CCCCTCCTGC TGCAGCTGGG GCCCGAGCCC CCCGCCCCGC ACCCCGGGGT AAGCAAGCCC 
TGGAGATCTG GCGCCCCCCC GGAGGAGCCC GAGCGGCTGC CCCTCCACGT GCGGTTCCTG 
GAGAACTGCC AGCCCCGGGC CGGCGGGGCG GGTGGAAGGG GGCCCCCCTC GGAGGACGGG 
CCGGGGGTCC CGCCGCCCAG CCCGCGCCGC TCTGTGCCGC CCTCGCCGAC CTCCCCGCGG 
GGCAGTGAAG AGAACGGGCT CCCCCTGCTG GTCCTGCCGC CGCCCGCCCC CTCGGTAGAC 
GTGGAAGACG GCGAATTCCT CTTTGTGGAA CCGCTGCCCC CGCCCCTCGA GTTTTCCAAC 
AGCTTCGAGA AGCCGGAGTC GCCTCTCACG CCGGGGCCTC CCCACCCGCT GCCGGACCCA 
CCCACCCCGA CCACCCCATT GCCCTCTGTG CCGCCCCCGG CTGTGGCCGC CGCCCCTCCC 
ACCCTGGACT CGACCGCATC CAGCCTGACT TCCTATGACA GCGAGGTGGC AACGCTGACC 
CAGGGGGCCC CCGCGGCTCC TGGGGACCCC CCTCCGCCAG GCCCTCCCGC CCCAGCCGCT 
CCGGCTCCCC CAGCCCCCCA GCCTGGCCCA GACCCTCCCC CGGGCACGGA TTCTGGTATT 
GAGGAGGTGG ACAGTCGGAG CAGCAGCGAC CACCCCCTGG AGACCATCAG CAGCGCCTCC 
ACGCTGAGCA GCCTGTCCGC TGAAGGCGGG GGCGGCGCAG GGGGTGGAAC TGGCGGCGTT 
GGAGGCAGTG GTGGCGCCAG CGTGGCCGGT GGACCAGAGC TCCTGGATAC CTACGTGGCC 
TACTTGGATG GCCAGGCCTT CGGGGGCAGC GGCACTCCTG GCCCCCCATA CCCCCCGCAG 
CTCATGACTC CCTCTAAGCT CCGGGGCCGG GCGCTCGGGG CCGGCGGAGG CCTGCGGCTG 
GGCCCCGGTG GGGGACTCCG AGACCCTATC ACTCCCACCA GCCCCACTGT CTCGGTGACG 
GGGGCGGGAA CCGATGGGCT GCTGGCCCTG AGCGGTTGTC CGGGATCCTC GACGGCAGGT 
GTGGCTGGCG GCCCCGTGGC TGTAGAGCCG GAAGGTCCGC CAGTGCCCTT GCCGTCGGCC 
TCCTCCCTCC CTCGAAAGCT GCTGCCCTGG GAGGAGGGCC CGGGCCCGCC GCCGCCCCCC 
CTGCCGGGGC CCTTAGCCCA ACCTCAGGCC TCAGCCTTGG CCACTGTAAA AGCCAGCATC 
ATCAGTGAAC TCAGCTCCAA GCTTCAGCAG TTTGGGGGCT CCTCGGCAGC TGGTGGCGCT 
CTGCCCTGGG CCCGGGGAGG CAGCGGGGGA AGCGGGGACA GCCACCACGG GGGAGCCAGC 
TACGTCCCCG AGCGGACTTC CTCCCTGCAG CGGCAGAGAC TCTCCGAAGA CTCCCAGTCC 
TCGCTCCTCT CCAAACCCGT GAGCAGCCTG TTTCAGAACT GGTCCAAACC ACCTCTGCCA 
CCACTCCCCA CGGGAACCGC GGTGTCCCCT TCAGCCGCTG CAGCCCCGGG GGCCACCTCC 
CCATCGGCTT CCTCCTCCTC CACGTCCGCC CGCCACCTCC AGGGCGTGGA GTTCGAGATG 
CGCCCACCTT TGCTCCGCCG GGCCCCCAGC CCCTCATTGC TGCCCGCCTC GGAGCACAAG 
GTCAGCCCTG CGCCCCGGCC CTCGTCCCTA CCCATCCTGC CTTCCGGACC CCTCTACCCG 
GGCCTCTTTG ACATCCGAGG CTCCCCCACC GGAGGGGCAG GAGGCTCGGC CGACCCCTTT 
GCCCCCGTCT TTGTACCGCC GCACCCCGGG ATATCCGGGG GTCTCGGCGG GGCCTTGTCA 
GGGGCCTCCC GCTCCCTCTC ACCGACCCGC CTGCTTTCCC TGCCCCCGGA CAAGCCGTTC 
GGCGCTAAAC CTCTGGGCTT CTGGACCAAG TTCGATGTGG CTGACTGGCT AGAGTGGCTG 
GGCTTGGCCG AACACCGGGC CCGGTTCCTG GACCACGAGA TCGATGGCTC CCACCTTCCC 
GCCTTGACCA AGGAGGACTA CGTCGATTTG GGGGTGACCA GGGTGGGACA CCGCATGAAC 
ATCGACCGGG CTCTCAAGTT TTTCCTGGAG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SHANK1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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