ACSBG1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACSBG1
Entrez Gene ID	515577
Full Name	acyl-CoA synthetase bubblegum family member 1
Synonyms	BG1
General protein information	Preferred Names acyl-CoA synthetase bubblegum family member 1 Names long-chain-fatty-acid--CoA ligase ACSBG1 lipidosin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010817065.2	XP_010815367.1	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X3
XM_024981756.1	XP_024837524.1	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X2
XM_015459239.1	XP_015314725.1	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X1
XM_005221946.3	XP_005222003.1	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X2
XM_024981755.1	XP_024837523.1	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X1
NM_001024548.1	NP_001019719.1	long-chain-fatty-acid--CoA ligase ACSBG1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100006023	XP_001344904.2
Homo sapiens (human)	ACSBG1	NP_055977.3
Macaca mulatta (Rhesus monkey)	LOC709676	XP_002804950.1
Bos taurus (cattle)	ACSBG1	NP_001019719.1
Canis lupus familiaris (dog)	ACSBG1	XP_005618497.1
Rattus norvegicus (Norway rat)	Acsbg1	NP_599216.1
Pan troglodytes (chimpanzee)	ACSBG1	XP_510525.3
Mus musculus (house mouse)	Acsbg1	NP_444408.1
Gallus gallus (chicken)	ACSBG1	XP_413747.3

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACSBG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACSBG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43090	NM_001024548.1 Latest version!	Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa228310	XM_024981755.1 Latest version!	Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa228311	XM_024981756.1 Latest version!	Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa30946	XM_005221946.3 Latest version!	Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa49336	XM_010817065.2 Latest version!	Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa94230	XM_015459239.1 Latest version!	Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACSBG1 ( NM_001024548.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001019719.1 Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), mRNA.

Price & Availability↑

CloneID	OBa43090
Clone ID Related Accession (Same CDS sequence)	NM_001024548.1
Accession Version	NM_001024548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase ACSBG1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT021864.1. On May 29, 2005 this sequence version replaced XM_593618.1. ##Evidence-Data-START## Transcript exon combination :: BT021864.1, BC112858.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGACA GCAGAGCAGC CCCGCAGGAG AGCCTGCTGG ATGCAAGCCT GGGGACCACC 
CAAGAAAACG TGGGAACCAG CTCCCTGACT GATGGGCAGA CGCTCTCCAA AGAGCCCCTA 
AGCCATGCTC TCAAACTCTC GACCCCAGAG AAGGTGAAAG ATGCCCAGTT GAATCCTCCA 
GAGGAGGCGC TATGGACGAC TCGGGCCGAC GGGCGCGTCC GCCTACGCAT AGACCCCATC 
TGCTCGCAGA CTCCCCGCAC TGTTCATCAG ATGTTCTCCA CGACTCTGGA CAAGTATGGG 
GACCTCAGTG CTATGGGCTT CAAGAGGCAG GGCACGTGGG AACACATCTC CTACACCCAG 
TACTACCTGC TGGCCCGCAA AGCTGCCAAG GGCTTCCTGA AGCTCGGCCT GGAGCGGGCC 
CACAGCGTGG CCATCCTCGC CTTCAACTCC CCGGAGTGGT TCTTCTCAGC AGTGGGCGCA 
GTCTTCGGAG GTGGCATCAT CACCGGCATC TACACGACCA GCTCCCCTGA GGCCTGTCAG 
TATATCGCCT ATGACTGCCG TGCCAACATC ATCGTGGTCG ACACACAGAA ACAGCTGGAA 
AAGATCCTGA AGATCTGGAA ACACCTACCA CATCTGAAGG CAGTAGTGAT ATATAGAGAA 
GCGCCTCCCA TGCGGATGCC CAGCGTGTAC ACGATGGAGG AGCTCATGGA GCTGGGGAAT 
GAGGTGCCTG AGGAGGCCCT GGACGTCATC ATCAACGCCC AGAAGCCTAA CCAGTGCTGT 
GCATTGGTCT ACACGTCGGG CACCACCGGG AACCCCAAGG GTGTGATGCT GAGTCAGGAC 
AATATCACGT GGACAGCACG GTACGGCAGC CAGGCCGGTG ACATCCAACC TGCGGAAATC 
CAACAGGAGG TGGTGGTCAG CTACCTGCCT CTCAGCCACA TTGCCGCCCA GATCTATGAC 
CTGTGGACAG GCATCCAGTG GGGGGCCCAG GTCTGCTTTG CCGAGCCTGA TGCTCTGAAG 
GGGAGTCTGG TGAACACGCT GCGGGAGGTG GAGCCCACGT CCCACATGGG GGTGCCTCGG 
GTGTGGGAGA AGATCATGGA GCAGATCCAG GAGGTGGCGG CTCAGTCTGG CTTCATCTGG 
CGCAAGATGC TGCTCTGGGC CATGTCGGTG ACCTTGGAGC AGAACCTCAC CTGCCCGAGC 
AGCGATCTGA AGCCCTTCAC AACCCGACTG GCAGATTACC TGGTGCTAGC CAAGGTCCGC 
CAGGCGCTGG GCTTTGCCAA GTGTCAGAAG AACTTCTACG GGGCAGCCCC CATGACTGCG 
GAGACACAGC ACTTCTTCCT GGGCCTCAAC ATCCGCTTGT ATGCAGGCTA CGGCCTCAGC 
GAGACCTCAG GCCCCCACTT CATGTCCAGC CCCTACAACT ACCGACTCTA TAGTTCCGGC 
AAGGTGGTGC CAGGTTGCCA GGTGAAGCTG GTGAATGAGG ACGCAGAGGG CATCGGGGAG 
ATCTGCCTGT GGGGCCGCAC CATCTTCATG GGCTACCTGA ACATGGAGGA CAAGACGTGT 
GAGGCCATTG ACGCCGAGGG CTGGCTGCAC ACTGGAGACA CGGGGCGCCT GGATGCCGAT 
GGCTTCCTCT ATATCACGGG GCGTCTCAAA GAGTTAATCA TCACAGCTGG TGGGGAGAAC 
GTGCCCCCTG TGCCCATTGA GGAGGCCGTG AAGACGGAGC TGCCAATCAT CAGGAATGCC 
ATGCTGATTG GAGACCAGAG GAAGTTCCTG TCCATGCTGC TAACCTTGAA GTGCACTCTG 
GACCCTGACA CCTTTGAGCC CACGGATAAT CTGACGGAAC AAGCTGTAGA GTTCTGCCAG 
AGGGTTGGCA GCAAAGCCAC CACGGTATCT GAGGTTGTGG GGAAGAAGGA TGAGGCCGTG 
TATCAGGCCA TCGAAGAAGG GATCCAGAGA GTCAACATGA ATGCAGCTGC ACGACCCTAC 
CACATCCAGA AGTGGGCCAT TCTTGAGAAG GACTTCTCCA TTTCAGGTGG AGAGTTGGGT 
CCCACAATGA AACTGAAACG GCTCGCAGTC CTGGAGAAGT ACAAAGATGT CATCGATTCC 
TTTTACCAAG AGCAAAAAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019719.1
CDS	64..2187
Translation	MPDSRAAPQESLLDASLGTTQENVGTSSLTDGQTLSKEPLSHALKLSTPEKVKDAQLNPPEEALWTTRADGRVRLRIDPICSQTPRTVHQMFSTTLDKYGDLSAMGFKRQGTWEHISYTQYYLLARKAAKGFLKLGLERAHSVAILAFNSPEWFFSAVGAVFGGGIITGIYTTSSPEACQYIAYDCRANIIVVDTQKQLEKILKIWKHLPHLKAVVIYREAPPMRMPSVYTMEELMELGNEVPEEALDVIINAQKPNQCCALVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEIQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMEQIQEVAAQSGFIWRKMLLWAMSVTLEQNLTCPSSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMTAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKVVPGCQVKLVNEDAEGIGEICLWGRTIFMGYLNMEDKTCEAIDAEGWLHTGDTGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKTELPIIRNAMLIGDQRKFLSMLLTLKCTLDPDTFEPTDNLTEQAVEFCQRVGSKATTVSEVVGKKDEAVYQAIEEGIQRVNMNAAARPYHIQKWAILEKDFSISGGELGPTMKLKRLAVLEKYKDVIDSFYQEQKK

Target ORF information:

RefSeq Version	NM_001024548.1
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024548.1

ORF Insert Sequence:

ATGCCGGACA GCAGAGCAGC CCCGCAGGAG AGCCTGCTGG ATGCAAGCCT GGGGACCACC 
CAAGAAAACG TGGGAACCAG CTCCCTGACT GATGGGCAGA CGCTCTCCAA AGAGCCCCTA 
AGCCATGCTC TCAAACTCTC GACCCCAGAG AAGGTGAAAG ATGCCCAGTT GAATCCTCCA 
GAGGAGGCGC TATGGACGAC TCGGGCCGAC GGGCGCGTCC GCCTACGCAT AGACCCCATC 
TGCTCGCAGA CTCCCCGCAC TGTTCATCAG ATGTTCTCCA CGACTCTGGA CAAGTATGGG 
GACCTCAGTG CTATGGGCTT CAAGAGGCAG GGCACGTGGG AACACATCTC CTACACCCAG 
TACTACCTGC TGGCCCGCAA AGCTGCCAAG GGCTTCCTGA AGCTCGGCCT GGAGCGGGCC 
CACAGCGTGG CCATCCTCGC CTTCAACTCC CCGGAGTGGT TCTTCTCAGC AGTGGGCGCA 
GTCTTCGGAG GTGGCATCAT CACCGGCATC TACACGACCA GCTCCCCTGA GGCCTGTCAG 
TATATCGCCT ATGACTGCCG TGCCAACATC ATCGTGGTCG ACACACAGAA ACAGCTGGAA 
AAGATCCTGA AGATCTGGAA ACACCTACCA CATCTGAAGG CAGTAGTGAT ATATAGAGAA 
GCGCCTCCCA TGCGGATGCC CAGCGTGTAC ACGATGGAGG AGCTCATGGA GCTGGGGAAT 
GAGGTGCCTG AGGAGGCCCT GGACGTCATC ATCAACGCCC AGAAGCCTAA CCAGTGCTGT 
GCATTGGTCT ACACGTCGGG CACCACCGGG AACCCCAAGG GTGTGATGCT GAGTCAGGAC 
AATATCACGT GGACAGCACG GTACGGCAGC CAGGCCGGTG ACATCCAACC TGCGGAAATC 
CAACAGGAGG TGGTGGTCAG CTACCTGCCT CTCAGCCACA TTGCCGCCCA GATCTATGAC 
CTGTGGACAG GCATCCAGTG GGGGGCCCAG GTCTGCTTTG CCGAGCCTGA TGCTCTGAAG 
GGGAGTCTGG TGAACACGCT GCGGGAGGTG GAGCCCACGT CCCACATGGG GGTGCCTCGG 
GTGTGGGAGA AGATCATGGA GCAGATCCAG GAGGTGGCGG CTCAGTCTGG CTTCATCTGG 
CGCAAGATGC TGCTCTGGGC CATGTCGGTG ACCTTGGAGC AGAACCTCAC CTGCCCGAGC 
AGCGATCTGA AGCCCTTCAC AACCCGACTG GCAGATTACC TGGTGCTAGC CAAGGTCCGC 
CAGGCGCTGG GCTTTGCCAA GTGTCAGAAG AACTTCTACG GGGCAGCCCC CATGACTGCG 
GAGACACAGC ACTTCTTCCT GGGCCTCAAC ATCCGCTTGT ATGCAGGCTA CGGCCTCAGC 
GAGACCTCAG GCCCCCACTT CATGTCCAGC CCCTACAACT ACCGACTCTA TAGTTCCGGC 
AAGGTGGTGC CAGGTTGCCA GGTGAAGCTG GTGAATGAGG ACGCAGAGGG CATCGGGGAG 
ATCTGCCTGT GGGGCCGCAC CATCTTCATG GGCTACCTGA ACATGGAGGA CAAGACGTGT 
GAGGCCATTG ACGCCGAGGG CTGGCTGCAC ACTGGAGACA CGGGGCGCCT GGATGCCGAT 
GGCTTCCTCT ATATCACGGG GCGTCTCAAA GAGTTAATCA TCACAGCTGG TGGGGAGAAC 
GTGCCCCCTG TGCCCATTGA GGAGGCCGTG AAGACGGAGC TGCCAATCAT CAGGAATGCC 
ATGCTGATTG GAGACCAGAG GAAGTTCCTG TCCATGCTGC TAACCTTGAA GTGCACTCTG 
GACCCTGACA CCTTTGAGCC CACGGATAAT CTGACGGAAC AAGCTGTAGA GTTCTGCCAG 
AGGGTTGGCA GCAAAGCCAC CACGGTATCT GAGGTTGTGG GGAAGAAGGA TGAGGCCGTG 
TATCAGGCCA TCGAAGAAGG GATCCAGAGA GTCAACATGA ATGCAGCTGC ACGACCCTAC 
CACATCCAGA AGTGGGCCAT TCTTGAGAAG GACTTCTCCA TTTCAGGTGG AGAGTTGGGT 
CCCACAATGA AACTGAAACG GCTCGCAGTC CTGGAGAAGT ACAAAGATGT CATCGATTCC 
TTTTACCAAG AGCAAAAAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSBG1 ( XM_024981755.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837523.1 Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa228310
Clone ID Related Accession (Same CDS sequence)	XM_024981755.1
Accession Version	XM_024981755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACA GCAGAGCAGC CCCGCAGGAG AGCCTGCTGG ATGCAAGCCT GGGGACCACC 
CAAGAAAACG TGGGAACCAG GTTCTTAAAC AGACTACCGC CAGGTGGGCC TGAGTGTGTT 
CGCAAACTCA GTTTGAACCT GCGGTACCAG CAAGAAGTAA GAGCGAATGT GAAAAGCAGC 
TCCCTGACTG ATGGGCAGAC GCTCTCCAAA GAGCCCCTAA GCCATGCTCT CAAACTCTCG 
ACCCCAGAGA AGGTGAAAGA TGCCCAGTTG AATCCTCCAG AGGAGGCGCT ATGGACGACT 
CGGGCCGACG GGCGCGTCCG CCTACGCATA GACCCCATCT GCTCGCAGAC TCCCCGCACT 
GTTCATCAGA TGTTCTCCAC GACTCTGGAC AAGTATGGGG ACCTCAGTGC TATGGGCTTC 
AAGAGGCAGG GCACGTGGGA ACACATCTCC TACACCCAGT ACTACCTGCT GGCCCGCAAA 
GCTGCCAAGG GCTTCCTGAA GCTCGGCCTG GAGCGGGCCC ACAGCGTGGC CATCCTCGCC 
TTCAACTCCC CGGAGTGGTT CTTCTCAGCA GTGGGCGCAG TCTTCGGAGG TGGCATCATC 
ACCGGCATCT ACACGACCAG CTCCCCTGAG GCCTGTCAGT ATATCGCCTA TGACTGCCGT 
GCCAACATCA TCGTGGTCGA CACACAGAAA CAGCTGGAAA AGATCCTGAA GATCTGGAAA 
CACCTACCAC ATCTGAAGGC AGTAGTGATA TATAGAGAAG CGCCTCCCAT GCGGATGCCC 
AGCGTGTACA CGATGGAGGA GCTCATGGAG CTGGGGAATG AGGTGCCTGA GGAGGCCCTG 
GACGTCATCA TCAACGCCCA GAAGCCTAAC CAGTGCTGTG CATTGGTCTA CACGTCGGGC 
ACCACCGGGA ACCCCAAGGG TGTGATGCTG AGTCAGGACA ATATCACGTG GACAGCACGG 
TACGGCAGCC AGGCCGGTGA CATCCAACCT GCGGAAATCC AACAGGAGGT GGTGGTCAGC 
TACCTGCCTC TCAGCCACAT TGCCGCCCAG ATCTATGACC TGTGGACAGG CATCCAGTGG 
GGGGCCCAGG TCTGCTTTGC CGAGCCTGAT GCTCTGAAGG GGAGTCTGGT GAACACGCTG 
CGGGAGGTGG AGCCCACGTC CCACATGGGG GTGCCTCGGG TGTGGGAGAA GATCATGGAG 
CAGATCCAGG AGGTGGCGGC TCAGTCTGGC TTCATCTGGC GCAAGATGCT GCTCTGGGCC 
ATGTCGGTGA CCTTGGAGCA GAACCTCACC TGCCCGAGCA GCGATCTGAA GCCCTTCACA 
ACCCGACTGG CAGATTACCT GGTGCTAGCC AAGGTCCGCC AGGCGCTGGG CTTTGCCAAG 
TGTCAGAAGA ACTTCTACGG GGCAGCCCCC ATGACTGCGG AGACACAGCA CTTCTTCCTG 
GGCCTCAACA TCCGCTTGTA TGCAGGCTAC GGCCTCAGCG AGACCTCAGG CCCCCACTTC 
ATGTCCAGCC CCTACAACTA CCGACTCTAT AGTTCCGGCA AGGTGGTGCC AGGTTGCCAG 
GTGAAGCTGG TGAATGAGGA CGCAGAGGGC ATCGGGGAGA TCTGCCTGTG GGGCCGCACC 
ATCTTCATGG GCTACCTGAA CATGGAGGAC AAGACGTGTG AGGCCATTGA CGCCGAGGGC 
TGGCTGCACA CCGGAGACAC GGGGCGCCTG GATGCCGATG GCTTCCTCTA TATCACGGGG 
CGTCTCAAAG AGTTAATCAT CACAGCTGGT GGGGAGAACG TGCCCCCTGT GCCCATTGAG 
GAGGCCGTGA AGACGGAGCT GCCAATCATC AGGAATGCCA TGCTGATTGG AGACCAGAGG 
AAGTTCCTGT CCATGCTGCT AACCTTGAAG TGCACTCTGG ACCCTGACAC CTTTGAGCCC 
ACGGATAATC TGACGGAACA AGCTGTAGAG TTCTGCCAGA GGGTTGGCAG CAAAGCCACC 
ACGGTATCTG AGGTTGTGGG GAAGAAGGAT GAGGCCGTGT ATCAGGCCAT CGAAGAAGGG 
ATCCAGAGAG TCAACATGAA TGCAGCTGCA CGACCCTACC ACATCCAGAA GTGGGCCATT 
CTTGAGAAGG ACTTCTCCAT TTCAGGTGGA GAGTTGGGTC CCACAATGAA ACTGAAGCGG 
CTCGCAGTCC TGGAGAAGTA CAAAGATGTC ATCGATTCCT TTTACCAAGA GCAAAAAAAT 
TTTCAGGTAG ATTTAACACG CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837523.1
CDS	104..2350
Translation	MPDSRAAPQESLLDASLGTTQENVGTRFLNRLPPGGPECVRKLSLNLRYQQEVRANVKSSSLTDGQTLSKEPLSHALKLSTPEKVKDAQLNPPEEALWTTRADGRVRLRIDPICSQTPRTVHQMFSTTLDKYGDLSAMGFKRQGTWEHISYTQYYLLARKAAKGFLKLGLERAHSVAILAFNSPEWFFSAVGAVFGGGIITGIYTTSSPEACQYIAYDCRANIIVVDTQKQLEKILKIWKHLPHLKAVVIYREAPPMRMPSVYTMEELMELGNEVPEEALDVIINAQKPNQCCALVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEIQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMEQIQEVAAQSGFIWRKMLLWAMSVTLEQNLTCPSSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMTAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKVVPGCQVKLVNEDAEGIGEICLWGRTIFMGYLNMEDKTCEAIDAEGWLHTGDTGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKTELPIIRNAMLIGDQRKFLSMLLTLKCTLDPDTFEPTDNLTEQAVEFCQRVGSKATTVSEVVGKKDEAVYQAIEEGIQRVNMNAAARPYHIQKWAILEKDFSISGGELGPTMKLKRLAVLEKYKDVIDSFYQEQKNFQVDLTRC

Target ORF information:

RefSeq Version	XM_024981755.1
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981755.1

ORF Insert Sequence:

ATGCCGGACA GCAGAGCAGC CCCGCAGGAG AGCCTGCTGG ATGCAAGCCT GGGGACCACC 
CAAGAAAACG TGGGAACCAG GTTCTTAAAC AGACTACCGC CAGGTGGGCC TGAGTGTGTT 
CGCAAACTCA GTTTGAACCT GCGGTACCAG CAAGAAGTAA GAGCGAATGT GAAAAGCAGC 
TCCCTGACTG ATGGGCAGAC GCTCTCCAAA GAGCCCCTAA GCCATGCTCT CAAACTCTCG 
ACCCCAGAGA AGGTGAAAGA TGCCCAGTTG AATCCTCCAG AGGAGGCGCT ATGGACGACT 
CGGGCCGACG GGCGCGTCCG CCTACGCATA GACCCCATCT GCTCGCAGAC TCCCCGCACT 
GTTCATCAGA TGTTCTCCAC GACTCTGGAC AAGTATGGGG ACCTCAGTGC TATGGGCTTC 
AAGAGGCAGG GCACGTGGGA ACACATCTCC TACACCCAGT ACTACCTGCT GGCCCGCAAA 
GCTGCCAAGG GCTTCCTGAA GCTCGGCCTG GAGCGGGCCC ACAGCGTGGC CATCCTCGCC 
TTCAACTCCC CGGAGTGGTT CTTCTCAGCA GTGGGCGCAG TCTTCGGAGG TGGCATCATC 
ACCGGCATCT ACACGACCAG CTCCCCTGAG GCCTGTCAGT ATATCGCCTA TGACTGCCGT 
GCCAACATCA TCGTGGTCGA CACACAGAAA CAGCTGGAAA AGATCCTGAA GATCTGGAAA 
CACCTACCAC ATCTGAAGGC AGTAGTGATA TATAGAGAAG CGCCTCCCAT GCGGATGCCC 
AGCGTGTACA CGATGGAGGA GCTCATGGAG CTGGGGAATG AGGTGCCTGA GGAGGCCCTG 
GACGTCATCA TCAACGCCCA GAAGCCTAAC CAGTGCTGTG CATTGGTCTA CACGTCGGGC 
ACCACCGGGA ACCCCAAGGG TGTGATGCTG AGTCAGGACA ATATCACGTG GACAGCACGG 
TACGGCAGCC AGGCCGGTGA CATCCAACCT GCGGAAATCC AACAGGAGGT GGTGGTCAGC 
TACCTGCCTC TCAGCCACAT TGCCGCCCAG ATCTATGACC TGTGGACAGG CATCCAGTGG 
GGGGCCCAGG TCTGCTTTGC CGAGCCTGAT GCTCTGAAGG GGAGTCTGGT GAACACGCTG 
CGGGAGGTGG AGCCCACGTC CCACATGGGG GTGCCTCGGG TGTGGGAGAA GATCATGGAG 
CAGATCCAGG AGGTGGCGGC TCAGTCTGGC TTCATCTGGC GCAAGATGCT GCTCTGGGCC 
ATGTCGGTGA CCTTGGAGCA GAACCTCACC TGCCCGAGCA GCGATCTGAA GCCCTTCACA 
ACCCGACTGG CAGATTACCT GGTGCTAGCC AAGGTCCGCC AGGCGCTGGG CTTTGCCAAG 
TGTCAGAAGA ACTTCTACGG GGCAGCCCCC ATGACTGCGG AGACACAGCA CTTCTTCCTG 
GGCCTCAACA TCCGCTTGTA TGCAGGCTAC GGCCTCAGCG AGACCTCAGG CCCCCACTTC 
ATGTCCAGCC CCTACAACTA CCGACTCTAT AGTTCCGGCA AGGTGGTGCC AGGTTGCCAG 
GTGAAGCTGG TGAATGAGGA CGCAGAGGGC ATCGGGGAGA TCTGCCTGTG GGGCCGCACC 
ATCTTCATGG GCTACCTGAA CATGGAGGAC AAGACGTGTG AGGCCATTGA CGCCGAGGGC 
TGGCTGCACA CCGGAGACAC GGGGCGCCTG GATGCCGATG GCTTCCTCTA TATCACGGGG 
CGTCTCAAAG AGTTAATCAT CACAGCTGGT GGGGAGAACG TGCCCCCTGT GCCCATTGAG 
GAGGCCGTGA AGACGGAGCT GCCAATCATC AGGAATGCCA TGCTGATTGG AGACCAGAGG 
AAGTTCCTGT CCATGCTGCT AACCTTGAAG TGCACTCTGG ACCCTGACAC CTTTGAGCCC 
ACGGATAATC TGACGGAACA AGCTGTAGAG TTCTGCCAGA GGGTTGGCAG CAAAGCCACC 
ACGGTATCTG AGGTTGTGGG GAAGAAGGAT GAGGCCGTGT ATCAGGCCAT CGAAGAAGGG 
ATCCAGAGAG TCAACATGAA TGCAGCTGCA CGACCCTACC ACATCCAGAA GTGGGCCATT 
CTTGAGAAGG ACTTCTCCAT TTCAGGTGGA GAGTTGGGTC CCACAATGAA ACTGAAGCGG 
CTCGCAGTCC TGGAGAAGTA CAAAGATGTC ATCGATTCCT TTTACCAAGA GCAAAAAAAT 
TTTCAGGTAG ATTTAACACG CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSBG1 ( XM_024981756.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837524.1 Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X2, mRNA.

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CloneID	OBa228311
Clone ID Related Accession (Same CDS sequence)	XM_024981756.1
Accession Version	XM_024981756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACA GCAGAGCAGC CCCGCAGGAG AGCCTGCTGG ATGCAAGCCT GGGGACCACC 
CAAGAAAACG TGGGAACCAG CTCCCTGACT GATGGGCAGA CGCTCTCCAA AGAGCCCCTA 
AGCCATGCTC TCAAACTCTC GACCCCAGAG AAGGTGAAAG ATGCCCAGTT GAATCCTCCA 
GAGGAGGCGC TATGGACGAC TCGGGCCGAC GGGCGCGTCC GCCTACGCAT AGACCCCATC 
TGCTCGCAGA CTCCCCGCAC TGTTCATCAG ATGTTCTCCA CGACTCTGGA CAAGTATGGG 
GACCTCAGTG CTATGGGCTT CAAGAGGCAG GGCACGTGGG AACACATCTC CTACACCCAG 
TACTACCTGC TGGCCCGCAA AGCTGCCAAG GGCTTCCTGA AGCTCGGCCT GGAGCGGGCC 
CACAGCGTGG CCATCCTCGC CTTCAACTCC CCGGAGTGGT TCTTCTCAGC AGTGGGCGCA 
GTCTTCGGAG GTGGCATCAT CACCGGCATC TACACGACCA GCTCCCCTGA GGCCTGTCAG 
TATATCGCCT ATGACTGCCG TGCCAACATC ATCGTGGTCG ACACACAGAA ACAGCTGGAA 
AAGATCCTGA AGATCTGGAA ACACCTACCA CATCTGAAGG CAGTAGTGAT ATATAGAGAA 
GCGCCTCCCA TGCGGATGCC CAGCGTGTAC ACGATGGAGG AGCTCATGGA GCTGGGGAAT 
GAGGTGCCTG AGGAGGCCCT GGACGTCATC ATCAACGCCC AGAAGCCTAA CCAGTGCTGT 
GCATTGGTCT ACACGTCGGG CACCACCGGG AACCCCAAGG GTGTGATGCT GAGTCAGGAC 
AATATCACGT GGACAGCACG GTACGGCAGC CAGGCCGGTG ACATCCAACC TGCGGAAATC 
CAACAGGAGG TGGTGGTCAG CTACCTGCCT CTCAGCCACA TTGCCGCCCA GATCTATGAC 
CTGTGGACAG GCATCCAGTG GGGGGCCCAG GTCTGCTTTG CCGAGCCTGA TGCTCTGAAG 
GGGAGTCTGG TGAACACGCT GCGGGAGGTG GAGCCCACGT CCCACATGGG GGTGCCTCGG 
GTGTGGGAGA AGATCATGGA GCAGATCCAG GAGGTGGCGG CTCAGTCTGG CTTCATCTGG 
CGCAAGATGC TGCTCTGGGC CATGTCGGTG ACCTTGGAGC AGAACCTCAC CTGCCCGAGC 
AGCGATCTGA AGCCCTTCAC AACCCGACTG GCAGATTACC TGGTGCTAGC CAAGGTCCGC 
CAGGCGCTGG GCTTTGCCAA GTGTCAGAAG AACTTCTACG GGGCAGCCCC CATGACTGCG 
GAGACACAGC ACTTCTTCCT GGGCCTCAAC ATCCGCTTGT ATGCAGGCTA CGGCCTCAGC 
GAGACCTCAG GCCCCCACTT CATGTCCAGC CCCTACAACT ACCGACTCTA TAGTTCCGGC 
AAGGTGGTGC CAGGTTGCCA GGTGAAGCTG GTGAATGAGG ACGCAGAGGG CATCGGGGAG 
ATCTGCCTGT GGGGCCGCAC CATCTTCATG GGCTACCTGA ACATGGAGGA CAAGACGTGT 
GAGGCCATTG ACGCCGAGGG CTGGCTGCAC ACCGGAGACA CGGGGCGCCT GGATGCCGAT 
GGCTTCCTCT ATATCACGGG GCGTCTCAAA GAGTTAATCA TCACAGCTGG TGGGGAGAAC 
GTGCCCCCTG TGCCCATTGA GGAGGCCGTG AAGACGGAGC TGCCAATCAT CAGGAATGCC 
ATGCTGATTG GAGACCAGAG GAAGTTCCTG TCCATGCTGC TAACCTTGAA GTGCACTCTG 
GACCCTGACA CCTTTGAGCC CACGGATAAT CTGACGGAAC AAGCTGTAGA GTTCTGCCAG 
AGGGTTGGCA GCAAAGCCAC CACGGTATCT GAGGTTGTGG GGAAGAAGGA TGAGGCCGTG 
TATCAGGCCA TCGAAGAAGG GATCCAGAGA GTCAACATGA ATGCAGCTGC ACGACCCTAC 
CACATCCAGA AGTGGGCCAT TCTTGAGAAG GACTTCTCCA TTTCAGGTGG AGAGTTGGGT 
CCCACAATGA AACTGAAGCG GCTCGCAGTC CTGGAGAAGT ACAAAGATGT CATCGATTCC 
TTTTACCAAG AGCAAAAAAA TTTTCAGGTA GATTTAACAC GCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837524.1
CDS	104..2251
Translation	MPDSRAAPQESLLDASLGTTQENVGTSSLTDGQTLSKEPLSHALKLSTPEKVKDAQLNPPEEALWTTRADGRVRLRIDPICSQTPRTVHQMFSTTLDKYGDLSAMGFKRQGTWEHISYTQYYLLARKAAKGFLKLGLERAHSVAILAFNSPEWFFSAVGAVFGGGIITGIYTTSSPEACQYIAYDCRANIIVVDTQKQLEKILKIWKHLPHLKAVVIYREAPPMRMPSVYTMEELMELGNEVPEEALDVIINAQKPNQCCALVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEIQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMEQIQEVAAQSGFIWRKMLLWAMSVTLEQNLTCPSSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMTAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKVVPGCQVKLVNEDAEGIGEICLWGRTIFMGYLNMEDKTCEAIDAEGWLHTGDTGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKTELPIIRNAMLIGDQRKFLSMLLTLKCTLDPDTFEPTDNLTEQAVEFCQRVGSKATTVSEVVGKKDEAVYQAIEEGIQRVNMNAAARPYHIQKWAILEKDFSISGGELGPTMKLKRLAVLEKYKDVIDSFYQEQKNFQVDLTRC

Target ORF information:

RefSeq Version	XM_024981756.1
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981756.1

ORF Insert Sequence:

ATGCCGGACA GCAGAGCAGC CCCGCAGGAG AGCCTGCTGG ATGCAAGCCT GGGGACCACC 
CAAGAAAACG TGGGAACCAG CTCCCTGACT GATGGGCAGA CGCTCTCCAA AGAGCCCCTA 
AGCCATGCTC TCAAACTCTC GACCCCAGAG AAGGTGAAAG ATGCCCAGTT GAATCCTCCA 
GAGGAGGCGC TATGGACGAC TCGGGCCGAC GGGCGCGTCC GCCTACGCAT AGACCCCATC 
TGCTCGCAGA CTCCCCGCAC TGTTCATCAG ATGTTCTCCA CGACTCTGGA CAAGTATGGG 
GACCTCAGTG CTATGGGCTT CAAGAGGCAG GGCACGTGGG AACACATCTC CTACACCCAG 
TACTACCTGC TGGCCCGCAA AGCTGCCAAG GGCTTCCTGA AGCTCGGCCT GGAGCGGGCC 
CACAGCGTGG CCATCCTCGC CTTCAACTCC CCGGAGTGGT TCTTCTCAGC AGTGGGCGCA 
GTCTTCGGAG GTGGCATCAT CACCGGCATC TACACGACCA GCTCCCCTGA GGCCTGTCAG 
TATATCGCCT ATGACTGCCG TGCCAACATC ATCGTGGTCG ACACACAGAA ACAGCTGGAA 
AAGATCCTGA AGATCTGGAA ACACCTACCA CATCTGAAGG CAGTAGTGAT ATATAGAGAA 
GCGCCTCCCA TGCGGATGCC CAGCGTGTAC ACGATGGAGG AGCTCATGGA GCTGGGGAAT 
GAGGTGCCTG AGGAGGCCCT GGACGTCATC ATCAACGCCC AGAAGCCTAA CCAGTGCTGT 
GCATTGGTCT ACACGTCGGG CACCACCGGG AACCCCAAGG GTGTGATGCT GAGTCAGGAC 
AATATCACGT GGACAGCACG GTACGGCAGC CAGGCCGGTG ACATCCAACC TGCGGAAATC 
CAACAGGAGG TGGTGGTCAG CTACCTGCCT CTCAGCCACA TTGCCGCCCA GATCTATGAC 
CTGTGGACAG GCATCCAGTG GGGGGCCCAG GTCTGCTTTG CCGAGCCTGA TGCTCTGAAG 
GGGAGTCTGG TGAACACGCT GCGGGAGGTG GAGCCCACGT CCCACATGGG GGTGCCTCGG 
GTGTGGGAGA AGATCATGGA GCAGATCCAG GAGGTGGCGG CTCAGTCTGG CTTCATCTGG 
CGCAAGATGC TGCTCTGGGC CATGTCGGTG ACCTTGGAGC AGAACCTCAC CTGCCCGAGC 
AGCGATCTGA AGCCCTTCAC AACCCGACTG GCAGATTACC TGGTGCTAGC CAAGGTCCGC 
CAGGCGCTGG GCTTTGCCAA GTGTCAGAAG AACTTCTACG GGGCAGCCCC CATGACTGCG 
GAGACACAGC ACTTCTTCCT GGGCCTCAAC ATCCGCTTGT ATGCAGGCTA CGGCCTCAGC 
GAGACCTCAG GCCCCCACTT CATGTCCAGC CCCTACAACT ACCGACTCTA TAGTTCCGGC 
AAGGTGGTGC CAGGTTGCCA GGTGAAGCTG GTGAATGAGG ACGCAGAGGG CATCGGGGAG 
ATCTGCCTGT GGGGCCGCAC CATCTTCATG GGCTACCTGA ACATGGAGGA CAAGACGTGT 
GAGGCCATTG ACGCCGAGGG CTGGCTGCAC ACCGGAGACA CGGGGCGCCT GGATGCCGAT 
GGCTTCCTCT ATATCACGGG GCGTCTCAAA GAGTTAATCA TCACAGCTGG TGGGGAGAAC 
GTGCCCCCTG TGCCCATTGA GGAGGCCGTG AAGACGGAGC TGCCAATCAT CAGGAATGCC 
ATGCTGATTG GAGACCAGAG GAAGTTCCTG TCCATGCTGC TAACCTTGAA GTGCACTCTG 
GACCCTGACA CCTTTGAGCC CACGGATAAT CTGACGGAAC AAGCTGTAGA GTTCTGCCAG 
AGGGTTGGCA GCAAAGCCAC CACGGTATCT GAGGTTGTGG GGAAGAAGGA TGAGGCCGTG 
TATCAGGCCA TCGAAGAAGG GATCCAGAGA GTCAACATGA ATGCAGCTGC ACGACCCTAC 
CACATCCAGA AGTGGGCCAT TCTTGAGAAG GACTTCTCCA TTTCAGGTGG AGAGTTGGGT 
CCCACAATGA AACTGAAGCG GCTCGCAGTC CTGGAGAAGT ACAAAGATGT CATCGATTCC 
TTTTACCAAG AGCAAAAAAA TTTTCAGGTA GATTTAACAC GCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSBG1 ( XM_005221946.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222003.1 Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X2, mRNA.

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CloneID	OBa30946
Clone ID Related Accession (Same CDS sequence)	XM_005221946.3
Accession Version	XM_005221946.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221946.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACA GCAGAGCAGC CCCGCAGGAG AGCCTGCTGG ATGCAAGCCT GGGGACCACC 
CAAGAAAACG TGGGAACCAG GTTCTTAAAC AGACTACCGC CAGGTGGGCC TGAGTGTGTT 
CGCAAACTCA GTTTGAACCT GCGGTACCAG CAAGAAGTAA GAGCGAATGT GAAAAGCAGC 
TCCCTGACTG ATGGGCAGAC GCTCTCCAAA GAGCCCCTAA GCCATGCTCT CAAACTCTCG 
ACCCCAGAGA AGGTGAAAGA TGCCCAGTTG AATCCTCCAG AGGAGGCGCT ATGGACGACT 
CGGGCCGACG GGCGCGTCCG CCTACGCATA GACCCCATCT GCTCGCAGAC TCCCCGCACT 
GTTCATCAGA TGTTCTCCAC GACTCTGGAC AAGTATGGGG ACCTCAGTGC TATGGGCTTC 
AAGAGGCAGG GCACGTGGGA ACACATCTCC TACACCCAGT ACTACCTGCT GGCCCGCAAA 
GCTGCCAAGG GCTTCCTGAA GCTCGGCCTG GAGCGGGCCC ACAGCGTGGC CATCCTCGCC 
TTCAACTCCC CGGAGTGGTT CTTCTCAGCA GTGGGCGCAG TCTTCGGAGG TGGCATCATC 
ACCGGCATCT ACACGACCAG CTCCCCTGAG GCCTGTCAGT ATATCGCCTA TGACTGCCGT 
GCCAACATCA TCGTGGTCGA CACACAGAAA CAGCTGGAAA AGATCCTGAA GATCTGGAAA 
CACCTACCAC ATCTGAAGGC AGTAGTGATA TATAGAGAAG CGCCTCCCAT GCGGATGCCC 
AGCGTGTACA CGATGGAGGA GCTCATGGAG CTGGGGAATG AGGTGCCTGA GGAGGCCCTG 
GACGTCATCA TCAACGCCCA GAAGCCTAAC CAGTGCTGTG CATTGGTCTA CACGTCGGGC 
ACCACCGGGA ACCCCAAGGG TGTGATGCTG AGTCAGGACA ATATCACGTG GACAGCACGG 
TACGGCAGCC AGGCCGGTGA CATCCAACCT GCGGAAATCC AACAGGAGGT GGTGGTCAGC 
TACCTGCCTC TCAGCCACAT TGCCGCCCAG ATCTATGACC TGTGGACAGG CATCCAGTGG 
GGGGCCCAGG TCTGCTTTGC CGAGCCTGAT GCTCTGAAGG GGAGTCTGGT GAACACGCTG 
CGGGAGGTGG AGCCCACGTC CCACATGGGG GTGCCTCGGG TGTGGGAGAA GATCATGGAG 
CAGATCCAGG AGGTGGCGGC TCAGTCTGGC TTCATCTGGC GCAAGATGCT GCTCTGGGCC 
ATGTCGGTGA CCTTGGAGCA GAACCTCACC TGCCCGAGCA GCGATCTGAA GCCCTTCACA 
ACCCGACTGG CAGATTACCT GGTGCTAGCC AAGGTCCGCC AGGCGCTGGG CTTTGCCAAG 
TGTCAGAAGA ACTTCTACGG GGCAGCCCCC ATGACTGCGG AGACACAGCA CTTCTTCCTG 
GGCCTCAACA TCCGCTTGTA TGCAGGCTAC GGCCTCAGCG AGACCTCAGG CCCCCACTTC 
ATGTCCAGCC CCTACAACTA CCGACTCTAT AGTTCCGGCA AGGTGGTGCC AGGTTGCCAG 
GTGAAGCTGG TGAATGAGGA CGCAGAGGGC ATCGGGGAGA TCTGCCTGTG GGGCCGCACC 
ATCTTCATGG GCTACCTGAA CATGGAGGAC AAGACGTGTG AGGCCATTGA CGCCGAGGGC 
TGGCTGCACA CCGGAGACAC GGGGCGCCTG GATGCCGATG GCTTCCTCTA TATCACGGGG 
CGTCTCAAAG AGTTAATCAT CACAGCTGGT GGGGAGAACG TGCCCCCTGT GCCCATTGAG 
GAGGCCGTGA AGACGGAGCT GCCAATCATC AGGAATGCCA TGCTGATTGG AGACCAGAGG 
AAGTTCCTGT CCATGCTGCT AACCTTGAAG TGCACTCTGG ACCCTGACAC CTTTGAGCCC 
ACGGATAATC TGACGGAACA AGCTGTAGAG TTCTGCCAGA GGGTTGGCAG CAAAGCCACC 
ACGGTATCTG AGGTTGTGGG GAAGAAGGAT GAGGCCGTGT ATCAGGCCAT CGAAGAAGGG 
ATCCAGAGAG TCAACATGAA TGCAGCTGCA CGACCCTACC ACATCCAGAA GTGGGCCATT 
CTTGAGAAGG ACTTCTCCAT TTCAGGTGGA GAGTTGGGTC CCACAATGAA ACTGAAACGG 
CTCGCAGTCC TGGAGAAGTA CAAAGATGTC ATCGATTCCT TTTACCAAGA GCAAAAAAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222003.1
CDS	104..2326
Translation	MPDSRAAPQESLLDASLGTTQENVGTRFLNRLPPGGPECVRKLSLNLRYQQEVRANVKSSSLTDGQTLSKEPLSHALKLSTPEKVKDAQLNPPEEALWTTRADGRVRLRIDPICSQTPRTVHQMFSTTLDKYGDLSAMGFKRQGTWEHISYTQYYLLARKAAKGFLKLGLERAHSVAILAFNSPEWFFSAVGAVFGGGIITGIYTTSSPEACQYIAYDCRANIIVVDTQKQLEKILKIWKHLPHLKAVVIYREAPPMRMPSVYTMEELMELGNEVPEEALDVIINAQKPNQCCALVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEIQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMEQIQEVAAQSGFIWRKMLLWAMSVTLEQNLTCPSSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMTAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKVVPGCQVKLVNEDAEGIGEICLWGRTIFMGYLNMEDKTCEAIDAEGWLHTGDTGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKTELPIIRNAMLIGDQRKFLSMLLTLKCTLDPDTFEPTDNLTEQAVEFCQRVGSKATTVSEVVGKKDEAVYQAIEEGIQRVNMNAAARPYHIQKWAILEKDFSISGGELGPTMKLKRLAVLEKYKDVIDSFYQEQKK

Target ORF information:

RefSeq Version	XM_005221946.3
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221946.3

ORF Insert Sequence:

ATGCCGGACA GCAGAGCAGC CCCGCAGGAG AGCCTGCTGG ATGCAAGCCT GGGGACCACC 
CAAGAAAACG TGGGAACCAG GTTCTTAAAC AGACTACCGC CAGGTGGGCC TGAGTGTGTT 
CGCAAACTCA GTTTGAACCT GCGGTACCAG CAAGAAGTAA GAGCGAATGT GAAAAGCAGC 
TCCCTGACTG ATGGGCAGAC GCTCTCCAAA GAGCCCCTAA GCCATGCTCT CAAACTCTCG 
ACCCCAGAGA AGGTGAAAGA TGCCCAGTTG AATCCTCCAG AGGAGGCGCT ATGGACGACT 
CGGGCCGACG GGCGCGTCCG CCTACGCATA GACCCCATCT GCTCGCAGAC TCCCCGCACT 
GTTCATCAGA TGTTCTCCAC GACTCTGGAC AAGTATGGGG ACCTCAGTGC TATGGGCTTC 
AAGAGGCAGG GCACGTGGGA ACACATCTCC TACACCCAGT ACTACCTGCT GGCCCGCAAA 
GCTGCCAAGG GCTTCCTGAA GCTCGGCCTG GAGCGGGCCC ACAGCGTGGC CATCCTCGCC 
TTCAACTCCC CGGAGTGGTT CTTCTCAGCA GTGGGCGCAG TCTTCGGAGG TGGCATCATC 
ACCGGCATCT ACACGACCAG CTCCCCTGAG GCCTGTCAGT ATATCGCCTA TGACTGCCGT 
GCCAACATCA TCGTGGTCGA CACACAGAAA CAGCTGGAAA AGATCCTGAA GATCTGGAAA 
CACCTACCAC ATCTGAAGGC AGTAGTGATA TATAGAGAAG CGCCTCCCAT GCGGATGCCC 
AGCGTGTACA CGATGGAGGA GCTCATGGAG CTGGGGAATG AGGTGCCTGA GGAGGCCCTG 
GACGTCATCA TCAACGCCCA GAAGCCTAAC CAGTGCTGTG CATTGGTCTA CACGTCGGGC 
ACCACCGGGA ACCCCAAGGG TGTGATGCTG AGTCAGGACA ATATCACGTG GACAGCACGG 
TACGGCAGCC AGGCCGGTGA CATCCAACCT GCGGAAATCC AACAGGAGGT GGTGGTCAGC 
TACCTGCCTC TCAGCCACAT TGCCGCCCAG ATCTATGACC TGTGGACAGG CATCCAGTGG 
GGGGCCCAGG TCTGCTTTGC CGAGCCTGAT GCTCTGAAGG GGAGTCTGGT GAACACGCTG 
CGGGAGGTGG AGCCCACGTC CCACATGGGG GTGCCTCGGG TGTGGGAGAA GATCATGGAG 
CAGATCCAGG AGGTGGCGGC TCAGTCTGGC TTCATCTGGC GCAAGATGCT GCTCTGGGCC 
ATGTCGGTGA CCTTGGAGCA GAACCTCACC TGCCCGAGCA GCGATCTGAA GCCCTTCACA 
ACCCGACTGG CAGATTACCT GGTGCTAGCC AAGGTCCGCC AGGCGCTGGG CTTTGCCAAG 
TGTCAGAAGA ACTTCTACGG GGCAGCCCCC ATGACTGCGG AGACACAGCA CTTCTTCCTG 
GGCCTCAACA TCCGCTTGTA TGCAGGCTAC GGCCTCAGCG AGACCTCAGG CCCCCACTTC 
ATGTCCAGCC CCTACAACTA CCGACTCTAT AGTTCCGGCA AGGTGGTGCC AGGTTGCCAG 
GTGAAGCTGG TGAATGAGGA CGCAGAGGGC ATCGGGGAGA TCTGCCTGTG GGGCCGCACC 
ATCTTCATGG GCTACCTGAA CATGGAGGAC AAGACGTGTG AGGCCATTGA CGCCGAGGGC 
TGGCTGCACA CCGGAGACAC GGGGCGCCTG GATGCCGATG GCTTCCTCTA TATCACGGGG 
CGTCTCAAAG AGTTAATCAT CACAGCTGGT GGGGAGAACG TGCCCCCTGT GCCCATTGAG 
GAGGCCGTGA AGACGGAGCT GCCAATCATC AGGAATGCCA TGCTGATTGG AGACCAGAGG 
AAGTTCCTGT CCATGCTGCT AACCTTGAAG TGCACTCTGG ACCCTGACAC CTTTGAGCCC 
ACGGATAATC TGACGGAACA AGCTGTAGAG TTCTGCCAGA GGGTTGGCAG CAAAGCCACC 
ACGGTATCTG AGGTTGTGGG GAAGAAGGAT GAGGCCGTGT ATCAGGCCAT CGAAGAAGGG 
ATCCAGAGAG TCAACATGAA TGCAGCTGCA CGACCCTACC ACATCCAGAA GTGGGCCATT 
CTTGAGAAGG ACTTCTCCAT TTCAGGTGGA GAGTTGGGTC CCACAATGAA ACTGAAACGG 
CTCGCAGTCC TGGAGAAGTA CAAAGATGTC ATCGATTCCT TTTACCAAGA GCAAAAAAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSBG1 ( XM_010817065.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815367.1 Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa49336
Clone ID Related Accession (Same CDS sequence)	XM_010817065.2
Accession Version	XM_010817065.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817065.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGAGG CACTCCTGCT CAGGTCTGCC AGCTCCATCC TGAGGACCGT GTTCTTAAAC 
AGACTACCGC CAGGTGGGCC TGAGTGTGTT CGCAAACTCA GTTTGAACCT GCGGTACCAG 
CAAGAAGTAA GAGCGAATGT GAAAAGCAGC TCCCTGACTG ATGGGCAGAC GCTCTCCAAA 
GAGCCCCTAA GCCATGCTCT CAAACTCTCG ACCCCAGAGA AGGTGAAAGA TGCCCAGTTG 
AATCCTCCAG AGGAGGCGCT ATGGACGACT CGGGCCGACG GGCGCGTCCG CCTACGCATA 
GACCCCATCT GCTCGCAGAC TCCCCGCACT GTTCATCAGA TGTTCTCCAC GACTCTGGAC 
AAGTATGGGG ACCTCAGTGC TATGGGCTTC AAGAGGCAGG GCACGTGGGA ACACATCTCC 
TACACCCAGT ACTACCTGCT GGCCCGCAAA GCTGCCAAGG GCTTCCTGAA GCTCGGCCTG 
GAGCGGGCCC ACAGCGTGGC CATCCTCGCC TTCAACTCCC CGGAGTGGTT CTTCTCAGCA 
GTGGGCGCAG TCTTCGGAGG TGGCATCATC ACCGGCATCT ACACGACCAG CTCCCCTGAG 
GCCTGTCAGT ATATCGCCTA TGACTGCCGT GCCAACATCA TCGTGGTCGA CACACAGAAA 
CAGCTGGAAA AGATCCTGAA GATCTGGAAA CACCTACCAC ATCTGAAGGC AGTAGTGATA 
TATAGAGAAG CGCCTCCCAT GCGGATGCCC AGCGTGTACA CGATGGAGGA GCTCATGGAG 
CTGGGGAATG AGGTGCCTGA GGAGGCCCTG GACGTCATCA TCAACGCCCA GAAGCCTAAC 
CAGTGCTGTG CATTGGTCTA CACGTCGGGC ACCACCGGGA ACCCCAAGGG TGTGATGCTG 
AGTCAGGACA ATATCACGTG GACAGCACGG TACGGCAGCC AGGCCGGTGA CATCCAACCT 
GCGGAAATCC AACAGGAGGT GGTGGTCAGC TACCTGCCTC TCAGCCACAT TGCCGCCCAG 
ATCTATGACC TGTGGACAGG CATCCAGTGG GGGGCCCAGG TCTGCTTTGC CGAGCCTGAT 
GCTCTGAAGG GGAGTCTGGT GAACACGCTG CGGGAGGTGG AGCCCACGTC CCACATGGGG 
GTGCCTCGGG TGTGGGAGAA GATCATGGAG CAGATCCAGG AGGTGGCGGC TCAGTCTGGC 
TTCATCTGGC GCAAGATGCT GCTCTGGGCC ATGTCGGTGA CCTTGGAGCA GAACCTCACC 
TGCCCGAGCA GCGATCTGAA GCCCTTCACA ACCCGACTGG CAGATTACCT GGTGCTAGCC 
AAGGTCCGCC AGGCGCTGGG CTTTGCCAAG TGTCAGAAGA ACTTCTACGG GGCAGCCCCC 
ATGACTGCGG AGACACAGCA CTTCTTCCTG GGCCTCAACA TCCGCTTGTA TGCAGGCTAC 
GGCCTCAGCG AGACCTCAGG CCCCCACTTC ATGTCCAGCC CCTACAACTA CCGACTCTAT 
AGTTCCGGCA AGGTGGTGCC AGGTTGCCAG GTGAAGCTGG TGAATGAGGA CGCAGAGGGC 
ATCGGGGAGA TCTGCCTGTG GGGCCGCACC ATCTTCATGG GCTACCTGAA CATGGAGGAC 
AAGACGTGTG AGGCCATTGA CGCCGAGGGC TGGCTGCACA CCGGAGACAC GGGGCGCCTG 
GATGCCGATG GCTTCCTCTA TATCACGGGG CGTCTCAAAG AGTTAATCAT CACAGCTGGT 
GGGGAGAACG TGCCCCCTGT GCCCATTGAG GAGGCCGTGA AGACGGAGCT GCCAATCATC 
AGGAATGCCA TGCTGATTGG AGACCAGAGG AAGTTCCTGT CCATGCTGCT AACCTTGAAG 
TGCACTCTGG ACCCTGACAC CTTTGAGCCC ACGGATAATC TGACGGAACA AGCTGTAGAG 
TTCTGCCAGA GGGTTGGCAG CAAAGCCACC ACGGTATCTG AGGTTGTGGG GAAGAAGGAT 
GAGGCCGTGT ATCAGGCCAT CGAAGAAGGG ATCCAGAGAG TCAACATGAA TGCAGCTGCA 
CGACCCTACC ACATCCAGAA GTGGGCCATT CTTGAGAAGG ACTTCTCCAT TTCAGGTGGA 
GAGTTGGGTC CCACAATGAA ACTGAAACGG CTCGCAGTCC TGGAGAAGTA CAAAGATGTC 
ATCGATTCCT TTTACCAAGA GCAAAAAAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815367.1
CDS	212..2404
Translation	MPEALLLRSASSILRTVFLNRLPPGGPECVRKLSLNLRYQQEVRANVKSSSLTDGQTLSKEPLSHALKLSTPEKVKDAQLNPPEEALWTTRADGRVRLRIDPICSQTPRTVHQMFSTTLDKYGDLSAMGFKRQGTWEHISYTQYYLLARKAAKGFLKLGLERAHSVAILAFNSPEWFFSAVGAVFGGGIITGIYTTSSPEACQYIAYDCRANIIVVDTQKQLEKILKIWKHLPHLKAVVIYREAPPMRMPSVYTMEELMELGNEVPEEALDVIINAQKPNQCCALVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEIQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMEQIQEVAAQSGFIWRKMLLWAMSVTLEQNLTCPSSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMTAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKVVPGCQVKLVNEDAEGIGEICLWGRTIFMGYLNMEDKTCEAIDAEGWLHTGDTGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKTELPIIRNAMLIGDQRKFLSMLLTLKCTLDPDTFEPTDNLTEQAVEFCQRVGSKATTVSEVVGKKDEAVYQAIEEGIQRVNMNAAARPYHIQKWAILEKDFSISGGELGPTMKLKRLAVLEKYKDVIDSFYQEQKK

Target ORF information:

RefSeq Version	XM_010817065.2
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817065.2

ORF Insert Sequence:

ATGCCAGAGG CACTCCTGCT CAGGTCTGCC AGCTCCATCC TGAGGACCGT GTTCTTAAAC 
AGACTACCGC CAGGTGGGCC TGAGTGTGTT CGCAAACTCA GTTTGAACCT GCGGTACCAG 
CAAGAAGTAA GAGCGAATGT GAAAAGCAGC TCCCTGACTG ATGGGCAGAC GCTCTCCAAA 
GAGCCCCTAA GCCATGCTCT CAAACTCTCG ACCCCAGAGA AGGTGAAAGA TGCCCAGTTG 
AATCCTCCAG AGGAGGCGCT ATGGACGACT CGGGCCGACG GGCGCGTCCG CCTACGCATA 
GACCCCATCT GCTCGCAGAC TCCCCGCACT GTTCATCAGA TGTTCTCCAC GACTCTGGAC 
AAGTATGGGG ACCTCAGTGC TATGGGCTTC AAGAGGCAGG GCACGTGGGA ACACATCTCC 
TACACCCAGT ACTACCTGCT GGCCCGCAAA GCTGCCAAGG GCTTCCTGAA GCTCGGCCTG 
GAGCGGGCCC ACAGCGTGGC CATCCTCGCC TTCAACTCCC CGGAGTGGTT CTTCTCAGCA 
GTGGGCGCAG TCTTCGGAGG TGGCATCATC ACCGGCATCT ACACGACCAG CTCCCCTGAG 
GCCTGTCAGT ATATCGCCTA TGACTGCCGT GCCAACATCA TCGTGGTCGA CACACAGAAA 
CAGCTGGAAA AGATCCTGAA GATCTGGAAA CACCTACCAC ATCTGAAGGC AGTAGTGATA 
TATAGAGAAG CGCCTCCCAT GCGGATGCCC AGCGTGTACA CGATGGAGGA GCTCATGGAG 
CTGGGGAATG AGGTGCCTGA GGAGGCCCTG GACGTCATCA TCAACGCCCA GAAGCCTAAC 
CAGTGCTGTG CATTGGTCTA CACGTCGGGC ACCACCGGGA ACCCCAAGGG TGTGATGCTG 
AGTCAGGACA ATATCACGTG GACAGCACGG TACGGCAGCC AGGCCGGTGA CATCCAACCT 
GCGGAAATCC AACAGGAGGT GGTGGTCAGC TACCTGCCTC TCAGCCACAT TGCCGCCCAG 
ATCTATGACC TGTGGACAGG CATCCAGTGG GGGGCCCAGG TCTGCTTTGC CGAGCCTGAT 
GCTCTGAAGG GGAGTCTGGT GAACACGCTG CGGGAGGTGG AGCCCACGTC CCACATGGGG 
GTGCCTCGGG TGTGGGAGAA GATCATGGAG CAGATCCAGG AGGTGGCGGC TCAGTCTGGC 
TTCATCTGGC GCAAGATGCT GCTCTGGGCC ATGTCGGTGA CCTTGGAGCA GAACCTCACC 
TGCCCGAGCA GCGATCTGAA GCCCTTCACA ACCCGACTGG CAGATTACCT GGTGCTAGCC 
AAGGTCCGCC AGGCGCTGGG CTTTGCCAAG TGTCAGAAGA ACTTCTACGG GGCAGCCCCC 
ATGACTGCGG AGACACAGCA CTTCTTCCTG GGCCTCAACA TCCGCTTGTA TGCAGGCTAC 
GGCCTCAGCG AGACCTCAGG CCCCCACTTC ATGTCCAGCC CCTACAACTA CCGACTCTAT 
AGTTCCGGCA AGGTGGTGCC AGGTTGCCAG GTGAAGCTGG TGAATGAGGA CGCAGAGGGC 
ATCGGGGAGA TCTGCCTGTG GGGCCGCACC ATCTTCATGG GCTACCTGAA CATGGAGGAC 
AAGACGTGTG AGGCCATTGA CGCCGAGGGC TGGCTGCACA CCGGAGACAC GGGGCGCCTG 
GATGCCGATG GCTTCCTCTA TATCACGGGG CGTCTCAAAG AGTTAATCAT CACAGCTGGT 
GGGGAGAACG TGCCCCCTGT GCCCATTGAG GAGGCCGTGA AGACGGAGCT GCCAATCATC 
AGGAATGCCA TGCTGATTGG AGACCAGAGG AAGTTCCTGT CCATGCTGCT AACCTTGAAG 
TGCACTCTGG ACCCTGACAC CTTTGAGCCC ACGGATAATC TGACGGAACA AGCTGTAGAG 
TTCTGCCAGA GGGTTGGCAG CAAAGCCACC ACGGTATCTG AGGTTGTGGG GAAGAAGGAT 
GAGGCCGTGT ATCAGGCCAT CGAAGAAGGG ATCCAGAGAG TCAACATGAA TGCAGCTGCA 
CGACCCTACC ACATCCAGAA GTGGGCCATT CTTGAGAAGG ACTTCTCCAT TTCAGGTGGA 
GAGTTGGGTC CCACAATGAA ACTGAAACGG CTCGCAGTCC TGGAGAAGTA CAAAGATGTC 
ATCGATTCCT TTTACCAAGA GCAAAAAAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSBG1 ( XM_015459239.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314725.1 Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X1, mRNA.

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CloneID	OBa94230
Clone ID Related Accession (Same CDS sequence)	XM_015459239.1
Accession Version	XM_015459239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCC CTGTGCCTAC CAGCCCCAAG GAGGAAGGTG AACACCCTGT GGCAGCCAGA 
TCCTCCCTGG AGGTGCACAG TCCATCACTG TTCTTAAACA GACTACCGCC AGGTGGGCCT 
GAGTGTGTTC GCAAACTCAG TTTGAACCTG CGGTACCAGC AAGAAGTAAG AGCGAATGTG 
AAAAGCAGCT CCCTGACTGA TGGGCAGACG CTCTCCAAAG AGCCCCTAAG CCATGCTCTC 
AAACTCTCGA CCCCAGAGAA GGTGAAAGAT GCCCAGTTGA ATCCTCCAGA GGAGGCGCTA 
TGGACGACTC GGGCCGACGG GCGCGTCCGC CTACGCATAG ACCCCATCTG CTCGCAGACT 
CCCCGCACTG TTCATCAGAT GTTCTCCACG ACTCTGGACA AGTATGGGGA CCTCAGTGCT 
ATGGGCTTCA AGAGGCAGGG CACGTGGGAA CACATCTCCT ACACCCAGTA CTACCTGCTG 
GCCCGCAAAG CTGCCAAGGG CTTCCTGAAG CTCGGCCTGG AGCGGGCCCA CAGCGTGGCC 
ATCCTCGCCT TCAACTCCCC GGAGTGGTTC TTCTCAGCAG TGGGCGCAGT CTTCGGAGGT 
GGCATCATCA CCGGCATCTA CACGACCAGC TCCCCTGAGG CCTGTCAGTA TATCGCCTAT 
GACTGCCGTG CCAACATCAT CGTGGTCGAC ACACAGAAAC AGCTGGAAAA GATCCTGAAG 
ATCTGGAAAC ACCTACCACA TCTGAAGGCA GTAGTGATAT ATAGAGAAGC GCCTCCCATG 
CGGATGCCCA GCGTGTACAC GATGGAGGAG CTCATGGAGC TGGGGAATGA GGTGCCTGAG 
GAGGCCCTGG ACGTCATCAT CAACGCCCAG AAGCCTAACC AGTGCTGTGC ATTGGTCTAC 
ACGTCGGGCA CCACCGGGAA CCCCAAGGGT GTGATGCTGA GTCAGGACAA TATCACGTGG 
ACAGCACGGT ACGGCAGCCA GGCCGGTGAC ATCCAACCTG CGGAAATCCA ACAGGAGGTG 
GTGGTCAGCT ACCTGCCTCT CAGCCACATT GCCGCCCAGA TCTATGACCT GTGGACAGGC 
ATCCAGTGGG GGGCCCAGGT CTGCTTTGCC GAGCCTGATG CTCTGAAGGG GAGTCTGGTG 
AACACGCTGC GGGAGGTGGA GCCCACGTCC CACATGGGGG TGCCTCGGGT GTGGGAGAAG 
ATCATGGAGC AGATCCAGGA GGTGGCGGCT CAGTCTGGCT TCATCTGGCG CAAGATGCTG 
CTCTGGGCCA TGTCGGTGAC CTTGGAGCAG AACCTCACCT GCCCGAGCAG CGATCTGAAG 
CCCTTCACAA CCCGACTGGC AGATTACCTG GTGCTAGCCA AGGTCCGCCA GGCGCTGGGC 
TTTGCCAAGT GTCAGAAGAA CTTCTACGGG GCAGCCCCCA TGACTGCGGA GACACAGCAC 
TTCTTCCTGG GCCTCAACAT CCGCTTGTAT GCAGGCTACG GCCTCAGCGA GACCTCAGGC 
CCCCACTTCA TGTCCAGCCC CTACAACTAC CGACTCTATA GTTCCGGCAA GGTGGTGCCA 
GGTTGCCAGG TGAAGCTGGT GAATGAGGAC GCAGAGGGCA TCGGGGAGAT CTGCCTGTGG 
GGCCGCACCA TCTTCATGGG CTACCTGAAC ATGGAGGACA AGACGTGTGA GGCCATTGAC 
GCCGAGGGCT GGCTGCACAC CGGAGACACG GGGCGCCTGG ATGCCGATGG CTTCCTCTAT 
ATCACGGGGC GTCTCAAAGA GTTAATCATC ACAGCTGGTG GGGAGAACGT GCCCCCTGTG 
CCCATTGAGG AGGCCGTGAA GACGGAGCTG CCAATCATCA GGAATGCCAT GCTGATTGGA 
GACCAGAGGA AGTTCCTGTC CATGCTGCTA ACCTTGAAGT GCACTCTGGA CCCTGACACC 
TTTGAGCCCA CGGATAATCT GACGGAACAA GCTGTAGAGT TCTGCCAGAG GGTTGGCAGC 
AAAGCCACCA CGGTATCTGA GGTTGTGGGG AAGAAGGATG AGGCCGTGTA TCAGGCCATC 
GAAGAAGGGA TCCAGAGAGT CAACATGAAT GCAGCTGCAC GACCCTACCA CATCCAGAAG 
TGGGCCATTC TTGAGAAGGA CTTCTCCATT TCAGGTGGAG AGTTGGGTCC CACAATGAAA 
CTGAAACGGC TCGCAGTCCT GGAGAAGTAC AAAGATGTCA TCGATTCCTT TTACCAAGAG 
CAAAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314725.1
CDS	33..2264
Translation	MLCPVPTSPKEEGEHPVAARSSLEVHSPSLFLNRLPPGGPECVRKLSLNLRYQQEVRANVKSSSLTDGQTLSKEPLSHALKLSTPEKVKDAQLNPPEEALWTTRADGRVRLRIDPICSQTPRTVHQMFSTTLDKYGDLSAMGFKRQGTWEHISYTQYYLLARKAAKGFLKLGLERAHSVAILAFNSPEWFFSAVGAVFGGGIITGIYTTSSPEACQYIAYDCRANIIVVDTQKQLEKILKIWKHLPHLKAVVIYREAPPMRMPSVYTMEELMELGNEVPEEALDVIINAQKPNQCCALVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEIQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMEQIQEVAAQSGFIWRKMLLWAMSVTLEQNLTCPSSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMTAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKVVPGCQVKLVNEDAEGIGEICLWGRTIFMGYLNMEDKTCEAIDAEGWLHTGDTGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKTELPIIRNAMLIGDQRKFLSMLLTLKCTLDPDTFEPTDNLTEQAVEFCQRVGSKATTVSEVVGKKDEAVYQAIEEGIQRVNMNAAARPYHIQKWAILEKDFSISGGELGPTMKLKRLAVLEKYKDVIDSFYQEQKK

Target ORF information:

RefSeq Version	XM_015459239.1
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459239.1

ORF Insert Sequence:

ATGCTGTGCC CTGTGCCTAC CAGCCCCAAG GAGGAAGGTG AACACCCTGT GGCAGCCAGA 
TCCTCCCTGG AGGTGCACAG TCCATCACTG TTCTTAAACA GACTACCGCC AGGTGGGCCT 
GAGTGTGTTC GCAAACTCAG TTTGAACCTG CGGTACCAGC AAGAAGTAAG AGCGAATGTG 
AAAAGCAGCT CCCTGACTGA TGGGCAGACG CTCTCCAAAG AGCCCCTAAG CCATGCTCTC 
AAACTCTCGA CCCCAGAGAA GGTGAAAGAT GCCCAGTTGA ATCCTCCAGA GGAGGCGCTA 
TGGACGACTC GGGCCGACGG GCGCGTCCGC CTACGCATAG ACCCCATCTG CTCGCAGACT 
CCCCGCACTG TTCATCAGAT GTTCTCCACG ACTCTGGACA AGTATGGGGA CCTCAGTGCT 
ATGGGCTTCA AGAGGCAGGG CACGTGGGAA CACATCTCCT ACACCCAGTA CTACCTGCTG 
GCCCGCAAAG CTGCCAAGGG CTTCCTGAAG CTCGGCCTGG AGCGGGCCCA CAGCGTGGCC 
ATCCTCGCCT TCAACTCCCC GGAGTGGTTC TTCTCAGCAG TGGGCGCAGT CTTCGGAGGT 
GGCATCATCA CCGGCATCTA CACGACCAGC TCCCCTGAGG CCTGTCAGTA TATCGCCTAT 
GACTGCCGTG CCAACATCAT CGTGGTCGAC ACACAGAAAC AGCTGGAAAA GATCCTGAAG 
ATCTGGAAAC ACCTACCACA TCTGAAGGCA GTAGTGATAT ATAGAGAAGC GCCTCCCATG 
CGGATGCCCA GCGTGTACAC GATGGAGGAG CTCATGGAGC TGGGGAATGA GGTGCCTGAG 
GAGGCCCTGG ACGTCATCAT CAACGCCCAG AAGCCTAACC AGTGCTGTGC ATTGGTCTAC 
ACGTCGGGCA CCACCGGGAA CCCCAAGGGT GTGATGCTGA GTCAGGACAA TATCACGTGG 
ACAGCACGGT ACGGCAGCCA GGCCGGTGAC ATCCAACCTG CGGAAATCCA ACAGGAGGTG 
GTGGTCAGCT ACCTGCCTCT CAGCCACATT GCCGCCCAGA TCTATGACCT GTGGACAGGC 
ATCCAGTGGG GGGCCCAGGT CTGCTTTGCC GAGCCTGATG CTCTGAAGGG GAGTCTGGTG 
AACACGCTGC GGGAGGTGGA GCCCACGTCC CACATGGGGG TGCCTCGGGT GTGGGAGAAG 
ATCATGGAGC AGATCCAGGA GGTGGCGGCT CAGTCTGGCT TCATCTGGCG CAAGATGCTG 
CTCTGGGCCA TGTCGGTGAC CTTGGAGCAG AACCTCACCT GCCCGAGCAG CGATCTGAAG 
CCCTTCACAA CCCGACTGGC AGATTACCTG GTGCTAGCCA AGGTCCGCCA GGCGCTGGGC 
TTTGCCAAGT GTCAGAAGAA CTTCTACGGG GCAGCCCCCA TGACTGCGGA GACACAGCAC 
TTCTTCCTGG GCCTCAACAT CCGCTTGTAT GCAGGCTACG GCCTCAGCGA GACCTCAGGC 
CCCCACTTCA TGTCCAGCCC CTACAACTAC CGACTCTATA GTTCCGGCAA GGTGGTGCCA 
GGTTGCCAGG TGAAGCTGGT GAATGAGGAC GCAGAGGGCA TCGGGGAGAT CTGCCTGTGG 
GGCCGCACCA TCTTCATGGG CTACCTGAAC ATGGAGGACA AGACGTGTGA GGCCATTGAC 
GCCGAGGGCT GGCTGCACAC CGGAGACACG GGGCGCCTGG ATGCCGATGG CTTCCTCTAT 
ATCACGGGGC GTCTCAAAGA GTTAATCATC ACAGCTGGTG GGGAGAACGT GCCCCCTGTG 
CCCATTGAGG AGGCCGTGAA GACGGAGCTG CCAATCATCA GGAATGCCAT GCTGATTGGA 
GACCAGAGGA AGTTCCTGTC CATGCTGCTA ACCTTGAAGT GCACTCTGGA CCCTGACACC 
TTTGAGCCCA CGGATAATCT GACGGAACAA GCTGTAGAGT TCTGCCAGAG GGTTGGCAGC 
AAAGCCACCA CGGTATCTGA GGTTGTGGGG AAGAAGGATG AGGCCGTGTA TCAGGCCATC 
GAAGAAGGGA TCCAGAGAGT CAACATGAAT GCAGCTGCAC GACCCTACCA CATCCAGAAG 
TGGGCCATTC TTGAGAAGGA CTTCTCCATT TCAGGTGGAG AGTTGGGTCC CACAATGAAA 
CTGAAACGGC TCGCAGTCCT GGAGAAGTAC AAAGATGTCA TCGATTCCTT TTACCAAGAG 
CAAAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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ACSBG1 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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