RASGEF1A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGEF1A
Entrez Gene ID	514877
Full Name	RasGEF domain family member 1A
General protein information	Preferred Names RasGEF domain family member 1A Names ras-GEF domain-containing family member 1A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010820457.3	XP_010818759.1	ras-GEF domain-containing family member 1A isoform X2
XM_010820457.2	XP_010818759.1	ras-GEF domain-containing family member 1A isoform X2
NM_001015601.1	NP_001015601.1	ras-GEF domain-containing family member 1A
XM_005226256.3	XP_005226313.1	ras-GEF domain-containing family member 1A isoform X1
XM_024986646.1	XP_024842414.1	ras-GEF domain-containing family member 1A isoform X2
XM_010820456.2	XP_010818758.1	ras-GEF domain-containing family member 1A isoform X2
XM_010820456.3	XP_010818758.1	ras-GEF domain-containing family member 1A isoform X2
XM_005226256.4	XP_005226313.1	ras-GEF domain-containing family member 1A isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rasgef1a	NP_081802.1
Rattus norvegicus (Norway rat)	Rasgef1a	XP_232315.6
Xenopus tropicalis (tropical clawed frog)	rasgef1a	NP_001039175.2
Canis lupus familiaris (dog)	RASGEF1A	XP_534955.4
Gallus gallus (chicken)	RASGEF1A	XP_004942024.1
Macaca mulatta (Rhesus monkey)	RASGEF1A	XP_002805664.1
Homo sapiens (human)	RASGEF1A	NP_001269791.1
Bos taurus (cattle)	RASGEF1A	NP_001015601.1
Pan troglodytes (chimpanzee)	RASGEF1A	XP_003312591.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RASGEF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGEF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa39340	NM_001015601.1 Latest version!	Bos taurus RasGEF domain family member 1A (RASGEF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa34132	XM_005226256.4 Latest version!	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa49109	XM_010820457.3 Latest version!	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa49109	XM_024986646.1 Latest version!	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa49109	XM_010820456.3 Latest version!	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OBa49109	XM_010820457.2	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa49109	XM_010820456.2	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa34132	XM_005226256.3	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGEF1A ( NM_001015601.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001015601.1 Bos taurus RasGEF domain family member 1A (RASGEF1A), mRNA.

Price & Availability↑

CloneID	OBa39340
Clone ID Related Accession (Same CDS sequence)	NM_001015601.1
Accession Version	NM_001015601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	ras-GEF domain-containing family member 1A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT020843.1. On or before Apr 21, 2005 this sequence version replaced XM_592791.1, XM_595865.1. ##Evidence-Data-START## Transcript exon combination :: BT020843.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

ATGGGGGAGC GAGGCGGTGG GGCCGGCGGC TCTGGGGACC TCATCTTCCA AGATGGACGT 
CTCATCTCGG GGTCCCTGGA GGCCCTGATG GAGCACCTGG TCCCCACAGT GGACTATTAC 
CCTGACAGGA CGTACATCTT CACGTTTCTC CTGAGCTCCC GGGTCTTCAT CCCCCCTCAT 
GACCTGCTGG CCCGCGTGGG GCAGCTCTGC GTGGAGCAGA GGCAGCAGCT GGAGGCTGGG 
TCCGAGAAGG CCAAGCTGAA GTCCTTCTCA GCCAAGATCG TGCAGCTGCT TAAGGAGTGG 
ACGGAGGCCT TCCCCTTTGA CTTCCAGGAC GAGAAGGCCA TGGCTGAGTT GAAGGCCATC 
ACCCATCGGG TTACCCAGTG TGATGAGGAG AACGGCACTG TGAAGAAGGC CATCTCCCAG 
ATGACGCAGA GCCTGCTGCT GTCCCTGGCT GCCCGGAGCC AGCTTCAGGA GCTGCGGGAG 
AAGCTCCGCT CACCGGCCAT GGACAAAGGG CCCGTCCTCA AGGCCAAGCC TCCAGCTGCT 
CAGAAGGACA TCCTGGGTGT GTGCTGCGAC CCCCTGGTGC TGGCCCAGCA GCTGACACAT 
ATCGAGCTGG AGAGAGTCAG CAGCATCCAC CCTGAAGACT TGATGCAGAT TGTCAGCCAC 
ATGGACTCCC GGGACAAGCA CAGGTGCCGA GGGGACATGA CCAAGACGTA CAGCCTGGAG 
GCCTACGACA ACTGGTTCAA CTGCCTCAGC ATGCTGGTGG CCACCGAGGT GTGCCGGGTA 
GTGAAGAAGA AGCACCGGAC CCGCATGCTG GAGTTCTTCA TTGACGTGGC CCGAGAGTGC 
TTCAACATCG GCAACTTCAA CTCCATGATG GCCATCATCT CTGGCATGAA CCTCAGTCCT 
GTGGCGAGGC TGAAGAAAAC GTGGTCCAAG GTTAAGACAG CAAAGTTCGA CGTCTTGGAG 
CACCACATGG ACCCGTCCAG CAATTTCTGC AACTACCGGA CAGCCCTGCA GGGGGCCACG 
CAGAGGTCCC AGACAGCCAA CAGCAGCCGG GAGAAGATCG TCATCCCCGT GTTCAACCTT 
TTCGTCAAGG ACATCTACTT CCTGCACAAA ATCCACACCA ACCACCTGCC CAATGGACAC 
ATTAACTTCA AGAAATTTTG GGAGATCTCC AGGCAGATCC ATGAGTTCAT GACATGGACA 
CAGGTGGAGT GTCCCTTTGA GAAGGACAAG AAGATTCAGA GTTATCTGCT GACGGCTCCA 
ATCTACAGCG AAGAAGCTCT CTTCATCGCC TCCTTTGAAA GTGAAGGTCC AGAAAACCAC 
ATGGAAAAGG ACAGCTGGAA GACCCTCAGG ACCACTCTCC TTAACAGAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015601.1
CDS	11..1384
Translation	MGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	NM_001015601.1
Organism	Bos taurus(cattle)
Definition	Bos taurus RasGEF domain family member 1A (RASGEF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015601.1

ORF Insert Sequence:

ATGGGGGAGC GAGGCGGTGG GGCCGGCGGC TCTGGGGACC TCATCTTCCA AGATGGACGT 
CTCATCTCGG GGTCCCTGGA GGCCCTGATG GAGCACCTGG TCCCCACAGT GGACTATTAC 
CCTGACAGGA CGTACATCTT CACGTTTCTC CTGAGCTCCC GGGTCTTCAT CCCCCCTCAT 
GACCTGCTGG CCCGCGTGGG GCAGCTCTGC GTGGAGCAGA GGCAGCAGCT GGAGGCTGGG 
TCCGAGAAGG CCAAGCTGAA GTCCTTCTCA GCCAAGATCG TGCAGCTGCT TAAGGAGTGG 
ACGGAGGCCT TCCCCTTTGA CTTCCAGGAC GAGAAGGCCA TGGCTGAGTT GAAGGCCATC 
ACCCATCGGG TTACCCAGTG TGATGAGGAG AACGGCACTG TGAAGAAGGC CATCTCCCAG 
ATGACGCAGA GCCTGCTGCT GTCCCTGGCT GCCCGGAGCC AGCTTCAGGA GCTGCGGGAG 
AAGCTCCGCT CACCGGCCAT GGACAAAGGG CCCGTCCTCA AGGCCAAGCC TCCAGCTGCT 
CAGAAGGACA TCCTGGGTGT GTGCTGCGAC CCCCTGGTGC TGGCCCAGCA GCTGACACAT 
ATCGAGCTGG AGAGAGTCAG CAGCATCCAC CCTGAAGACT TGATGCAGAT TGTCAGCCAC 
ATGGACTCCC GGGACAAGCA CAGGTGCCGA GGGGACATGA CCAAGACGTA CAGCCTGGAG 
GCCTACGACA ACTGGTTCAA CTGCCTCAGC ATGCTGGTGG CCACCGAGGT GTGCCGGGTA 
GTGAAGAAGA AGCACCGGAC CCGCATGCTG GAGTTCTTCA TTGACGTGGC CCGAGAGTGC 
TTCAACATCG GCAACTTCAA CTCCATGATG GCCATCATCT CTGGCATGAA CCTCAGTCCT 
GTGGCGAGGC TGAAGAAAAC GTGGTCCAAG GTTAAGACAG CAAAGTTCGA CGTCTTGGAG 
CACCACATGG ACCCGTCCAG CAATTTCTGC AACTACCGGA CAGCCCTGCA GGGGGCCACG 
CAGAGGTCCC AGACAGCCAA CAGCAGCCGG GAGAAGATCG TCATCCCCGT GTTCAACCTT 
TTCGTCAAGG ACATCTACTT CCTGCACAAA ATCCACACCA ACCACCTGCC CAATGGACAC 
ATTAACTTCA AGAAATTTTG GGAGATCTCC AGGCAGATCC ATGAGTTCAT GACATGGACA 
CAGGTGGAGT GTCCCTTTGA GAAGGACAAG AAGATTCAGA GTTATCTGCT GACGGCTCCA 
ATCTACAGCG AAGAAGCTCT CTTCATCGCC TCCTTTGAAA GTGAAGGTCC AGAAAACCAC 
ATGGAAAAGG ACAGCTGGAA GACCCTCAGG ACCACTCTCC TTAACAGAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_005226256.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226313.1 Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa34132
Clone ID Related Accession (Same CDS sequence)	XM_005226256.3 , XM_005226256.4
Accession Version	XM_005226256.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	ras-GEF domain-containing family member 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104621.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226256.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTGG AGCCCCAGGA AACTATGCCC CAGACGTCCG TGGTCTTCTC CAGCATCCTC 
GGGCCCAGCT GTAGTGGACA GGGGCAGCCC GGCATGGGGG AGCGAGGCGG TGGGGCCGGC 
GGCTCTGGGG ACCTCATCTT CCAAGATGGA CGTCTCATCT CGGGGTCCCT GGAGGCCCTG 
ATGGAGCACC TGGTCCCCAC AGTGGACTAT TACCCTGACA GGACGTACAT CTTCACGTTT 
CTCCTGAGCT CCCGGGTCTT CATCCCCCCT CATGACCTGC TGGCCCGCGT GGGGCAGCTC 
TGCGTGGAGC AGAGGCAGCA GCTGGAGGCT GGGTCCGAGA AGGCCAAGCT GAAGTCCTTC 
TCAGCCAAGA TCGTGCAGCT GCTTAAGGAG TGGACGGAGG CCTTCCCCTT TGACTTCCAG 
GACGAGAAGG CCATGGCTGA GTTGAAGGCC ATCACCCATC GGGTTACCCA GTGTGATGAG 
GAGAACGGCA CTGTGAAGAA GGCCATCTCC CAGATGACGC AGAGCCTGCT GCTGTCCCTG 
GCTGCCCGGA GCCAGCTTCA GGAGCTGCGG GAGAAGCTCC GCTCACCGGC CATGGACAAA 
GGGCCCGTCC TCAAGGCCAA GCCTCCAGCT GCTCAGAAGG ACATCCTGGG TGTGTGCTGC 
GACCCCCTGG TGCTGGCCCA GCAGCTGACA CATATCGAGC TGGAGAGAGT CAGCAGCATC 
CACCCTGAAG ACTTGATGCA GATTGTCAGC CACATGGACT CCCGGGACAA GCACAGGTGC 
CGAGGGGACA TGACCAAGAC GTACAGCCTG GAGGCCTATG ACAACTGGTT CAACTGCCTC 
AGCATGCTGG TGGCCACCGA GGTGTGCCGG GTAGTGAAGA AGAAGCACCG GACCCGCATG 
CTGGAGTTCT TCATTGACGT GGCCCGAGAG TGCTTCAACA TCGGCAACTT CAACTCCATG 
ATGGCCATCA TCTCTGGCAT GAACCTCAGT CCTGTGGCGA GGCTGAAGAA AACGTGGTCC 
AAGGTTAAGA CAGCAAAGTT CGACGTCTTG GAGCACCACA TGGACCCGTC CAGCAATTTC 
TGCAACTACC GGACAGCCCT GCAGGGGGCC ACGCAGAGGT CCCAGACAGC CAACAGTAGC 
CGGGAGAAGA TCGTCATCCC CGTGTTCAAC CTTTTCGTCA AGGACATCTA CTTCCTGCAC 
AAAATCCACA CCAACCACCT GCCCAATGGA CACATTAACT TCAAGAAATT TTGGGAGATC 
TCCAGGCAGA TCCATGAGTT CATGACATGG ACACAGGTGG AGTGTCCCTT TGAGAAGGAC 
AAGAAGATTC AGAGTTATCT GCTGACGGCT CCAATCTACA GCGAAGAAGC TCTCTTCATC 
GCCTCCTTTG AAAGTGAAGG TCCAGAAAAC CACATGGAAA AGGACAGCTG GAAGACCCTC 
AGGACCACTC TCCTTAACAG AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226313.1
CDS	94..1560
Translation	MFLEPQETMPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_005226256.3
Organism	Bos taurus(cattle)
Definition	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226256.3

ORF Insert Sequence:

ATGTTTCTGG AGCCCCAGGA AACTATGCCC CAGACGTCCG TGGTCTTCTC CAGCATCCTC 
GGGCCCAGCT GTAGTGGACA GGGGCAGCCC GGCATGGGGG AGCGAGGCGG TGGGGCCGGC 
GGCTCTGGGG ACCTCATCTT CCAAGATGGA CGTCTCATCT CGGGGTCCCT GGAGGCCCTG 
ATGGAGCACC TGGTCCCCAC AGTGGACTAT TACCCTGACA GGACGTACAT CTTCACGTTT 
CTCCTGAGCT CCCGGGTCTT CATCCCCCCT CATGACCTGC TGGCCCGCGT GGGGCAGCTC 
TGCGTGGAGC AGAGGCAGCA GCTGGAGGCT GGGTCCGAGA AGGCCAAGCT GAAGTCCTTC 
TCAGCCAAGA TCGTGCAGCT GCTTAAGGAG TGGACGGAGG CCTTCCCCTT TGACTTCCAG 
GACGAGAAGG CCATGGCTGA GTTGAAGGCC ATCACCCATC GGGTTACCCA GTGTGATGAG 
GAGAACGGCA CTGTGAAGAA GGCCATCTCC CAGATGACGC AGAGCCTGCT GCTGTCCCTG 
GCTGCCCGGA GCCAGCTTCA GGAGCTGCGG GAGAAGCTCC GCTCACCGGC CATGGACAAA 
GGGCCCGTCC TCAAGGCCAA GCCTCCAGCT GCTCAGAAGG ACATCCTGGG TGTGTGCTGC 
GACCCCCTGG TGCTGGCCCA GCAGCTGACA CATATCGAGC TGGAGAGAGT CAGCAGCATC 
CACCCTGAAG ACTTGATGCA GATTGTCAGC CACATGGACT CCCGGGACAA GCACAGGTGC 
CGAGGGGACA TGACCAAGAC GTACAGCCTG GAGGCCTATG ACAACTGGTT CAACTGCCTC 
AGCATGCTGG TGGCCACCGA GGTGTGCCGG GTAGTGAAGA AGAAGCACCG GACCCGCATG 
CTGGAGTTCT TCATTGACGT GGCCCGAGAG TGCTTCAACA TCGGCAACTT CAACTCCATG 
ATGGCCATCA TCTCTGGCAT GAACCTCAGT CCTGTGGCGA GGCTGAAGAA AACGTGGTCC 
AAGGTTAAGA CAGCAAAGTT CGACGTCTTG GAGCACCACA TGGACCCGTC CAGCAATTTC 
TGCAACTACC GGACAGCCCT GCAGGGGGCC ACGCAGAGGT CCCAGACAGC CAACAGTAGC 
CGGGAGAAGA TCGTCATCCC CGTGTTCAAC CTTTTCGTCA AGGACATCTA CTTCCTGCAC 
AAAATCCACA CCAACCACCT GCCCAATGGA CACATTAACT TCAAGAAATT TTGGGAGATC 
TCCAGGCAGA TCCATGAGTT CATGACATGG ACACAGGTGG AGTGTCCCTT TGAGAAGGAC 
AAGAAGATTC AGAGTTATCT GCTGACGGCT CCAATCTACA GCGAAGAAGC TCTCTTCATC 
GCCTCCTTTG AAAGTGAAGG TCCAGAAAAC CACATGGAAA AGGACAGCTG GAAGACCCTC 
AGGACCACTC TCCTTAACAG AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_005226256.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226313.1 Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa34132
Clone ID Related Accession (Same CDS sequence)	XM_005226256.3 , XM_005226256.4
Accession Version	XM_005226256.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ras-GEF domain-containing family member 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226256.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTGG AGCCCCAGGA AACTATGCCC CAGACGTCCG TGGTCTTCTC CAGCATCCTC 
GGGCCCAGCT GTAGTGGACA GGGGCAGCCC GGCATGGGGG AGCGAGGCGG TGGGGCCGGC 
GGCTCTGGGG ACCTCATCTT CCAAGATGGA CGTCTCATCT CGGGGTCCCT GGAGGCCCTG 
ATGGAGCACC TGGTCCCCAC AGTGGACTAT TACCCTGACA GGACGTACAT CTTCACGTTT 
CTCCTGAGCT CCCGGGTCTT CATCCCCCCT CATGACCTGC TGGCCCGCGT GGGGCAGCTC 
TGCGTGGAGC AGAGGCAGCA GCTGGAGGCT GGGTCCGAGA AGGCCAAGCT GAAGTCCTTC 
TCAGCCAAGA TCGTGCAGCT GCTTAAGGAG TGGACGGAGG CCTTCCCCTT TGACTTCCAG 
GACGAGAAGG CCATGGCTGA GTTGAAGGCC ATCACCCATC GGGTTACCCA GTGTGATGAG 
GAGAACGGCA CTGTGAAGAA GGCCATCTCC CAGATGACGC AGAGCCTGCT GCTGTCCCTG 
GCTGCCCGGA GCCAGCTTCA GGAGCTGCGG GAGAAGCTCC GCTCACCGGC CATGGACAAA 
GGGCCCGTCC TCAAGGCCAA GCCTCCAGCT GCTCAGAAGG ACATCCTGGG TGTGTGCTGC 
GACCCCCTGG TGCTGGCCCA GCAGCTGACA CATATCGAGC TGGAGAGAGT CAGCAGCATC 
CACCCTGAAG ACTTGATGCA GATTGTCAGC CACATGGACT CCCGGGACAA GCACAGGTGC 
CGAGGGGACA TGACCAAGAC GTACAGCCTG GAGGCCTATG ACAACTGGTT CAACTGCCTC 
AGCATGCTGG TGGCCACCGA GGTGTGCCGG GTAGTGAAGA AGAAGCACCG GACCCGCATG 
CTGGAGTTCT TCATTGACGT GGCCCGAGAG TGCTTCAACA TCGGCAACTT CAACTCCATG 
ATGGCCATCA TCTCTGGCAT GAACCTCAGT CCTGTGGCGA GGCTGAAGAA AACGTGGTCC 
AAGGTTAAGA CAGCAAAGTT CGACGTCTTG GAGCACCACA TGGACCCGTC CAGCAATTTC 
TGCAACTACC GGACAGCCCT GCAGGGGGCC ACGCAGAGGT CCCAGACAGC CAACAGTAGC 
CGGGAGAAGA TCGTCATCCC CGTGTTCAAC CTTTTCGTCA AGGACATCTA CTTCCTGCAC 
AAAATCCACA CCAACCACCT GCCCAATGGA CACATTAACT TCAAGAAATT TTGGGAGATC 
TCCAGGCAGA TCCATGAGTT CATGACATGG ACACAGGTGG AGTGTCCCTT TGAGAAGGAC 
AAGAAGATTC AGAGTTATCT GCTGACGGCT CCAATCTACA GCGAAGAAGC TCTCTTCATC 
GCCTCCTTTG AAAGTGAAGG TCCAGAAAAC CACATGGAAA AGGACAGCTG GAAGACCCTC 
AGGACCACTC TCCTTAACAG AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226313.1
CDS	121..1587
Translation	MFLEPQETMPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_005226256.4
Organism	Bos taurus(cattle)
Definition	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226256.4

ORF Insert Sequence:

ATGTTTCTGG AGCCCCAGGA AACTATGCCC CAGACGTCCG TGGTCTTCTC CAGCATCCTC 
GGGCCCAGCT GTAGTGGACA GGGGCAGCCC GGCATGGGGG AGCGAGGCGG TGGGGCCGGC 
GGCTCTGGGG ACCTCATCTT CCAAGATGGA CGTCTCATCT CGGGGTCCCT GGAGGCCCTG 
ATGGAGCACC TGGTCCCCAC AGTGGACTAT TACCCTGACA GGACGTACAT CTTCACGTTT 
CTCCTGAGCT CCCGGGTCTT CATCCCCCCT CATGACCTGC TGGCCCGCGT GGGGCAGCTC 
TGCGTGGAGC AGAGGCAGCA GCTGGAGGCT GGGTCCGAGA AGGCCAAGCT GAAGTCCTTC 
TCAGCCAAGA TCGTGCAGCT GCTTAAGGAG TGGACGGAGG CCTTCCCCTT TGACTTCCAG 
GACGAGAAGG CCATGGCTGA GTTGAAGGCC ATCACCCATC GGGTTACCCA GTGTGATGAG 
GAGAACGGCA CTGTGAAGAA GGCCATCTCC CAGATGACGC AGAGCCTGCT GCTGTCCCTG 
GCTGCCCGGA GCCAGCTTCA GGAGCTGCGG GAGAAGCTCC GCTCACCGGC CATGGACAAA 
GGGCCCGTCC TCAAGGCCAA GCCTCCAGCT GCTCAGAAGG ACATCCTGGG TGTGTGCTGC 
GACCCCCTGG TGCTGGCCCA GCAGCTGACA CATATCGAGC TGGAGAGAGT CAGCAGCATC 
CACCCTGAAG ACTTGATGCA GATTGTCAGC CACATGGACT CCCGGGACAA GCACAGGTGC 
CGAGGGGACA TGACCAAGAC GTACAGCCTG GAGGCCTATG ACAACTGGTT CAACTGCCTC 
AGCATGCTGG TGGCCACCGA GGTGTGCCGG GTAGTGAAGA AGAAGCACCG GACCCGCATG 
CTGGAGTTCT TCATTGACGT GGCCCGAGAG TGCTTCAACA TCGGCAACTT CAACTCCATG 
ATGGCCATCA TCTCTGGCAT GAACCTCAGT CCTGTGGCGA GGCTGAAGAA AACGTGGTCC 
AAGGTTAAGA CAGCAAAGTT CGACGTCTTG GAGCACCACA TGGACCCGTC CAGCAATTTC 
TGCAACTACC GGACAGCCCT GCAGGGGGCC ACGCAGAGGT CCCAGACAGC CAACAGTAGC 
CGGGAGAAGA TCGTCATCCC CGTGTTCAAC CTTTTCGTCA AGGACATCTA CTTCCTGCAC 
AAAATCCACA CCAACCACCT GCCCAATGGA CACATTAACT TCAAGAAATT TTGGGAGATC 
TCCAGGCAGA TCCATGAGTT CATGACATGG ACACAGGTGG AGTGTCCCTT TGAGAAGGAC 
AAGAAGATTC AGAGTTATCT GCTGACGGCT CCAATCTACA GCGAAGAAGC TCTCTTCATC 
GCCTCCTTTG AAAGTGAAGG TCCAGAAAAC CACATGGAAA AGGACAGCTG GAAGACCCTC 
AGGACCACTC TCCTTAACAG AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_010820457.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818759.1 Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa49109
Clone ID Related Accession (Same CDS sequence)	XM_010820457.3 , XM_010820457.2 , XM_024986646.1 , XM_010820456.2 , XM_010820456.3
Accession Version	XM_010820457.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ras-GEF domain-containing family member 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820457.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGCTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACGCAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGTGTG TGCTGCGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CTGAAGACTT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGACATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TTAAGACAGC AAAGTTCGAC 
GTCTTGGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACAGCCAAC AGTAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AATGGACACA TTAACTTCAA GAAATTTTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACGGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818759.1
CDS	201..1643
Translation	MPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_010820457.3
Organism	Bos taurus(cattle)
Definition	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820457.3

ORF Insert Sequence:

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGCTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACGCAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGTGTG TGCTGCGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CTGAAGACTT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGACATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TTAAGACAGC AAAGTTCGAC 
GTCTTGGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACAGCCAAC AGTAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AATGGACACA TTAACTTCAA GAAATTTTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACGGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_010820457.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818759.1 Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa49109
Clone ID Related Accession (Same CDS sequence)	XM_010820457.3 , XM_010820457.2 , XM_024986646.1 , XM_010820456.2 , XM_010820456.3
Accession Version	XM_010820457.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	ras-GEF domain-containing family member 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104621.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGCTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACGCAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGTGTG TGCTGCGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CTGAAGACTT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGACATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TTAAGACAGC AAAGTTCGAC 
GTCTTGGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACAGCCAAC AGTAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AATGGACACA TTAACTTCAA GAAATTTTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACGGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818759.1
CDS	86..1528
Translation	MPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_010820457.2
Organism	Bos taurus(cattle)
Definition	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820457.2

ORF Insert Sequence:

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGCTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACGCAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGTGTG TGCTGCGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CTGAAGACTT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGACATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TTAAGACAGC AAAGTTCGAC 
GTCTTGGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACAGCCAAC AGTAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AATGGACACA TTAACTTCAA GAAATTTTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACGGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_024986646.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842414.1 Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa49109
Clone ID Related Accession (Same CDS sequence)	XM_010820457.3 , XM_010820457.2 , XM_024986646.1 , XM_010820456.2 , XM_010820456.3
Accession Version	XM_024986646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ras-GEF domain-containing family member 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGCTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACGCAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGTGTG TGCTGCGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CTGAAGACTT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGACATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TTAAGACAGC AAAGTTCGAC 
GTCTTGGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACAGCCAAC AGTAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AATGGACACA TTAACTTCAA GAAATTTTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACGGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842414.1
CDS	233..1675
Translation	MPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_024986646.1
Organism	Bos taurus(cattle)
Definition	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986646.1

ORF Insert Sequence:

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGCTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACGCAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGTGTG TGCTGCGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CTGAAGACTT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGACATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TTAAGACAGC AAAGTTCGAC 
GTCTTGGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACAGCCAAC AGTAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AATGGACACA TTAACTTCAA GAAATTTTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACGGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_010820456.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818758.1 Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X2, mRNA.

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CloneID	OBa49109
Clone ID Related Accession (Same CDS sequence)	XM_010820457.3 , XM_010820457.2 , XM_024986646.1 , XM_010820456.2 , XM_010820456.3
Accession Version	XM_010820456.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	ras-GEF domain-containing family member 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104621.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820456.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGCTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACGCAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGTGTG TGCTGCGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CTGAAGACTT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGACATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TTAAGACAGC AAAGTTCGAC 
GTCTTGGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACAGCCAAC AGTAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AATGGACACA TTAACTTCAA GAAATTTTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACGGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818758.1
CDS	298..1740
Translation	MPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_010820456.2
Organism	Bos taurus(cattle)
Definition	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820456.2

ORF Insert Sequence:

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGCTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACGCAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGTGTG TGCTGCGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CTGAAGACTT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGACATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TTAAGACAGC AAAGTTCGAC 
GTCTTGGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACAGCCAAC AGTAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AATGGACACA TTAACTTCAA GAAATTTTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACGGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_010820456.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818758.1 Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X2, mRNA.

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CloneID	OBa49109
Clone ID Related Accession (Same CDS sequence)	XM_010820457.3 , XM_010820457.2 , XM_024986646.1 , XM_010820456.2 , XM_010820456.3
Accession Version	XM_010820456.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ras-GEF domain-containing family member 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820456.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGCTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACGCAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGTGTG TGCTGCGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CTGAAGACTT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGACATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TTAAGACAGC AAAGTTCGAC 
GTCTTGGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACAGCCAAC AGTAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AATGGACACA TTAACTTCAA GAAATTTTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACGGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818758.1
CDS	302..1744
Translation	MPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_010820456.3
Organism	Bos taurus(cattle)
Definition	Bos taurus RasGEF domain family member 1A (RASGEF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820456.3

ORF Insert Sequence:

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGCTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACGCAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGTGTG TGCTGCGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CTGAAGACTT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGACATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TTAAGACAGC AAAGTTCGAC 
GTCTTGGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACAGCCAAC AGTAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AATGGACACA TTAACTTCAA GAAATTTTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACGGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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RASGEF1A cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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