ITSN1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ITSN1
Entrez Gene ID	510879
Full Name	intersectin 1
General protein information	Preferred Names intersectin 1 Names intersectin-1 LOW QUALITY PROTEIN: intersectin-1 intersectin 1 (SH3 domain protein)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024993604.1	XP_024849372.1	intersectin-1 isoform X4
XM_024993605.1	XP_024849373.1	intersectin-1 isoform X5
XM_015465187.1	XP_015320673.1	LOW QUALITY PROTEIN: intersectin-1
XM_024993592.1	XP_024849360.1	intersectin-1 isoform X1
XM_024993593.1	XP_024849361.1	intersectin-1 isoform X1
XM_024993595.1	XP_024849363.1	intersectin-1 isoform X2
XM_024993603.1	XP_024849371.1	intersectin-1 isoform X3
XM_015461917.1	XP_015317403.1	LOW QUALITY PROTEIN: intersectin-1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ITSN1	NP_003015.2
Canis lupus familiaris (dog)	ITSN1	XP_005638937.1
Drosophila melanogaster (fruit fly)	Dap160	NP_001246118.1
Xenopus tropicalis (tropical clawed frog)	itsn1	XP_002939390.1
Bos taurus (cattle)	ITSN1	XP_002707731.1
Mus musculus (house mouse)	Itsn1	NP_034717.2
Gallus gallus (chicken)	ITSN1	XP_416715.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009037	XP_319789.4
Macaca mulatta (Rhesus monkey)	ITSN1	XP_002803192.1
Rattus norvegicus (Norway rat)	Itsn1	NP_001129568.1
Pan troglodytes (chimpanzee)	ITSN1	XP_001166935.1
Danio rerio (zebrafish)	itsn1	NP_997065.1
Caenorhabditis elegans (roundworm)	itsn-1	NP_001255927.1
Canis lupus familiaris (dog)	LOC100856635	XP_003640127.1

Gene Ontology

The following ITSN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITSN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219037	XM_024993605.1 Latest version!	Bos taurus intersectin 1 (ITSN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa47837	XM_024993604.1 Latest version!	Bos taurus intersectin 1 (ITSN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa02401	XM_024993592.1 Latest version!	Bos taurus intersectin 1 (ITSN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa02401	XM_024993593.1 Latest version!	Bos taurus intersectin 1 (ITSN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa02403	XM_024993603.1 Latest version!	Bos taurus intersectin 1 (ITSN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa47836	XM_024993595.1 Latest version!	Bos taurus intersectin 1 (ITSN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa87631	XM_015461917.1 Latest version!	Bos taurus intersectin 1 (ITSN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa87631	XM_015465187.1 Latest version!	Bos taurus intersectin 1 (ITSN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITSN1 ( XM_024993605.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849373.1 Bos taurus intersectin 1 (ITSN1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa219037
Clone ID Related Accession (Same CDS sequence)	XM_024993605.1
Accession Version	XM_024993605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	intersectin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAAATCGCGC AGGTCATTGC CTCCTACACA GCTACGGGTC CTGAACAGCT TACCCTGGCC 
CCCGGTCAGC TGATCCTGAT CCGGAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTA 
CAGGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTTCCTG CTAATTACGT CAAACTTTTA 
AGCCCTGGGA CAAGCAAAAT CACTCCAACA GACCCACCGA AGCCAACAGC TTTCCCAACA 
GTGTGCCAGG TGATCGGGAT GTACGACTAC ACCGCGCAGA ACGACGACGA GCTGGCCTTC 
AGCAAGGGCC AGGTCATCAA TGTCCTCAAC AAGGAGGACC CCGACTGGTG GAAGGGTGAA 
GTCCACGGGC AGGTGGGGCT CTTCCCGTCC AACTACGTGA AGTTGACCAC GGACACGGAC 
CCCAGCCAGC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849373.1
CDS	271..3705
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVCETPAPSSTTPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQ

Target ORF information:

RefSeq Version	XM_024993605.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intersectin 1 (ITSN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993605.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAAATCGCGC AGGTCATTGC CTCCTACACA GCTACGGGTC CTGAACAGCT TACCCTGGCC 
CCCGGTCAGC TGATCCTGAT CCGGAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTA 
CAGGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTTCCTG CTAATTACGT CAAACTTTTA 
AGCCCTGGGA CAAGCAAAAT CACTCCAACA GACCCACCGA AGCCAACAGC TTTCCCAACA 
GTGTGCCAGG TGATCGGGAT GTACGACTAC ACCGCGCAGA ACGACGACGA GCTGGCCTTC 
AGCAAGGGCC AGGTCATCAA TGTCCTCAAC AAGGAGGACC CCGACTGGTG GAAGGGTGAA 
GTCCACGGGC AGGTGGGGCT CTTCCCGTCC AACTACGTGA AGTTGACCAC GGACACGGAC 
CCCAGCCAGC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_024993604.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849372.1 Bos taurus intersectin 1 (ITSN1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa47837
Clone ID Related Accession (Same CDS sequence)	XM_024993604.1
Accession Version	XM_024993604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	intersectin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849372.1
CDS	302..3949
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVCETPAPSSTTPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQ

Target ORF information:

RefSeq Version	XM_024993604.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intersectin 1 (ITSN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993604.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_024993592.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849360.1 Bos taurus intersectin 1 (ITSN1), transcript variant X1, mRNA.

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CloneID	OBa02401
Clone ID Related Accession (Same CDS sequence)	XM_024993592.1 , XM_024993593.1
Accession Version	XM_024993592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAATGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCTTG 
TCAGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTGCAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAACGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACCTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAGCCCA TCTTCCACAT TTCCCACATT 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCAG CGTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC 
CTGGTCCGCT CCCAAAGAGC CACAGGCATC GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGTGA GGTGACCATG 
GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849360.1
CDS	278..5428
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVCETPAPSSTTPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_024993592.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intersectin 1 (ITSN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993592.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAATGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCTTG 
TCAGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTGCAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAACGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACCTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAGCCCA TCTTCCACAT TTCCCACATT 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCAG CGTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC 
CTGGTCCGCT CCCAAAGAGC CACAGGCATC GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGTGA GGTGACCATG 
GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_024993593.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849361.1 Bos taurus intersectin 1 (ITSN1), transcript variant X2, mRNA.

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CloneID	OBa02401
Clone ID Related Accession (Same CDS sequence)	XM_024993592.1 , XM_024993593.1
Accession Version	XM_024993593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAATGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCTTG 
TCAGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTGCAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAACGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACCTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAGCCCA TCTTCCACAT TTCCCACATT 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCAG CGTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC 
CTGGTCCGCT CCCAAAGAGC CACAGGCATC GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGTGA GGTGACCATG 
GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849361.1
CDS	195..5345
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVCETPAPSSTTPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_024993593.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intersectin 1 (ITSN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993593.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAATGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCTTG 
TCAGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTGCAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAACGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACCTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAGCCCA TCTTCCACAT TTCCCACATT 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCAG CGTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC 
CTGGTCCGCT CCCAAAGAGC CACAGGCATC GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGTGA GGTGACCATG 
GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_024993603.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849371.1 Bos taurus intersectin 1 (ITSN1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa02403
Clone ID Related Accession (Same CDS sequence)	XM_024993603.1
Accession Version	XM_024993603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	intersectin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAAATCGCGC AGGTCATTGC CTCCTACACA GCTACGGGTC CTGAACAGCT TACCCTGGCC 
CCCGGTCAGC TGATCCTGAT CCGGAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTA 
CAGGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTTCCTG CTAATTACGT CAAACTTTTA 
AGCCCTGGGA CAAGCAAAAT CACTCCAACA GACCCACCGA AGCCAACAGC TTTCCCAACA 
GTGTGCCAGG TGATCGGGAT GTACGACTAC ACCGCGCAGA ACGACGACGA GCTGGCCTTC 
AGCAAGGGCC AGGTCATCAA TGTCCTCAAC AAGGAGGACC CCGACTGGTG GAAGGGTGAA 
GTCCACGGGC AGGTGGGGCT CTTCCCGTCC AACTACGTGA AGTTGACCAC GGACACGGAC 
CCCAGCCAGC AATGGTGCTC AGACTTACAC CTCTTGGATA TGCTGACCCC AACTGAAAGA 
AAGCGACAGG GGTACATCCA CGAGCTCATC GTCACGGAGG AGAACTACGT GAATGACCTG 
CAGCTGGTCA CAGAGATCTT TCAGAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA 
GAGGGTGCTA TGATTTTTGT TAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTG 
AAAGCGCTGA GGGTCCGCAA GAAGATGTCT GGGGAGAAGA TGCCCGTGAA GATGATCGGA 
GACATCCTGA CGGCCCAGCT GCCACACATG CAGCCCTACA TCCGCTTCTG CAGCTGCCAG 
CTCAACGGGG CCGCCTTGAT CCAGCAGAAG ACTGACGAGG CCCCAGACTT CAAGGAGTTT 
GTCAAAAGAC TGGCCATGGA CCCACGCTGT AAGGGAATGC CCCTGTCTAG TTTTATACTG 
AAGCCTATGC AACGAGTAAC GAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACT 
CCTGAAAACC ACCCAGACCA CAGCCACCTG AAGCACGCCC TGGAGAAGGC GGAGGAGCTG 
TGCTCCCAGG TGAATGAAGG GGTGCGGGAG AAGGAGAACT CGGACCGACT GGAGTGGATC 
CAGGCCCACG TGCTGTGTGA AGGCTTGTCA GAGCAACTTG TGTTCAATTC CGTGACCAAC 
TGCCTGGGGC CGCGCAAGTT CCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTCTT CAACGACTTC CTGCTGCTGA CCCAAATCAT CAAGCCCCTG 
GGCTCCTCGG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAC 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTGAAGTTGC CCACTGACCC TTCTGGGGAT 
GAGCCCATCT TCCACATTTC CCACATTGAC CGAGTCTACA CCCTCCGAGC AGAGAGCATA 
AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCAGCGT CTGAACTCTA CATAGAGACA 
GAGAAGAAGA AGCGGGAGAA GGCGTACCTG GTCCGCTCCC AAAGAGCCAC AGGCATCGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATT GAGCTGAAAC CCTGTCGCTC CCATGGAAAG 
AGTAACCCGT ACTGTGAGGT GACCATGGGC TCCCAGTGTC ACATCACCAA GACGATCCAG 
GACACTCTGA ATCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCAGAGA CCTGGAGCAG 
GAGGTGCTGT GCATCACTGT GTTTGAGAGG GACCAGTTCT CGCCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGCGTGGC CGACATCAAG AAGGACCAGG GCTCCAAGGG TCCCGTTACG 
AAGTGTCTCC TGCTGCACGA AGTTCCCACG GGAGAGATCG TGGTCCGCTT GGACCTGCAG 
TTGTTCGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849371.1
CDS	279..5216
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVCETPAPSSTTPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_024993603.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intersectin 1 (ITSN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993603.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAAATCGCGC AGGTCATTGC CTCCTACACA GCTACGGGTC CTGAACAGCT TACCCTGGCC 
CCCGGTCAGC TGATCCTGAT CCGGAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTA 
CAGGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTTCCTG CTAATTACGT CAAACTTTTA 
AGCCCTGGGA CAAGCAAAAT CACTCCAACA GACCCACCGA AGCCAACAGC TTTCCCAACA 
GTGTGCCAGG TGATCGGGAT GTACGACTAC ACCGCGCAGA ACGACGACGA GCTGGCCTTC 
AGCAAGGGCC AGGTCATCAA TGTCCTCAAC AAGGAGGACC CCGACTGGTG GAAGGGTGAA 
GTCCACGGGC AGGTGGGGCT CTTCCCGTCC AACTACGTGA AGTTGACCAC GGACACGGAC 
CCCAGCCAGC AATGGTGCTC AGACTTACAC CTCTTGGATA TGCTGACCCC AACTGAAAGA 
AAGCGACAGG GGTACATCCA CGAGCTCATC GTCACGGAGG AGAACTACGT GAATGACCTG 
CAGCTGGTCA CAGAGATCTT TCAGAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA 
GAGGGTGCTA TGATTTTTGT TAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTG 
AAAGCGCTGA GGGTCCGCAA GAAGATGTCT GGGGAGAAGA TGCCCGTGAA GATGATCGGA 
GACATCCTGA CGGCCCAGCT GCCACACATG CAGCCCTACA TCCGCTTCTG CAGCTGCCAG 
CTCAACGGGG CCGCCTTGAT CCAGCAGAAG ACTGACGAGG CCCCAGACTT CAAGGAGTTT 
GTCAAAAGAC TGGCCATGGA CCCACGCTGT AAGGGAATGC CCCTGTCTAG TTTTATACTG 
AAGCCTATGC AACGAGTAAC GAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACT 
CCTGAAAACC ACCCAGACCA CAGCCACCTG AAGCACGCCC TGGAGAAGGC GGAGGAGCTG 
TGCTCCCAGG TGAATGAAGG GGTGCGGGAG AAGGAGAACT CGGACCGACT GGAGTGGATC 
CAGGCCCACG TGCTGTGTGA AGGCTTGTCA GAGCAACTTG TGTTCAATTC CGTGACCAAC 
TGCCTGGGGC CGCGCAAGTT CCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTCTT CAACGACTTC CTGCTGCTGA CCCAAATCAT CAAGCCCCTG 
GGCTCCTCGG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAC 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTGAAGTTGC CCACTGACCC TTCTGGGGAT 
GAGCCCATCT TCCACATTTC CCACATTGAC CGAGTCTACA CCCTCCGAGC AGAGAGCATA 
AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCAGCGT CTGAACTCTA CATAGAGACA 
GAGAAGAAGA AGCGGGAGAA GGCGTACCTG GTCCGCTCCC AAAGAGCCAC AGGCATCGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATT GAGCTGAAAC CCTGTCGCTC CCATGGAAAG 
AGTAACCCGT ACTGTGAGGT GACCATGGGC TCCCAGTGTC ACATCACCAA GACGATCCAG 
GACACTCTGA ATCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCAGAGA CCTGGAGCAG 
GAGGTGCTGT GCATCACTGT GTTTGAGAGG GACCAGTTCT CGCCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGCGTGGC CGACATCAAG AAGGACCAGG GCTCCAAGGG TCCCGTTACG 
AAGTGTCTCC TGCTGCACGA AGTTCCCACG GGAGAGATCG TGGTCCGCTT GGACCTGCAG 
TTGTTCGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_024993595.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849363.1 Bos taurus intersectin 1 (ITSN1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa47836
Clone ID Related Accession (Same CDS sequence)	XM_024993595.1
Accession Version	XM_024993595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	intersectin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATG ATGGAAGGAT GGATCAAGTG GAGTTTTCCA TTGCTATGAA ACTCATCAAG 
CTGAAGCTCC AAGGATATCA GCTCCCCTCT GCACTTCCCC CTGTCATGAA ACAGCAACCA 
GTTGCTATTT CTAGTGCACC AGCCTTTGGT ATGGGAGGTA TTGCCAGCAT GCCGCCGCTG 
ACCGCTGTTG CTCCTGTACC AATGGGCTCC ATTCCCGTTG TTGGGATGTC TCCCCCACTA 
GTGTCATCTG TTCCTGCAGC AGGGGTGCCC CCCCTGGCCA ATGGGGCGCC CCCGGTCATA 
CAGCCTCTGC CTGCATTTGC TCATCCTGCT GCCACATTGC CAAAGAGTTC TTCCTTTAGT 
AGATCTGGCC CAGGGTCGCA GTTAAACACA AAATTACAGA AGGCACAATC ATTTGATGTG 
GCCAGTGTCC CTCCTACAGC TGAGTGGGCT GTTCCTCAGT CATCCAGGCT GAAATACAGA 
CAGCTCTTCA ATAGTCATGA CAAAACCATG AGTGGACACC TAACAGGTCC CCAAGCAAGA 
ACTATTCTTA TGCAATCAAG TTTACCACAG GCTCAGCTGG CTTCAATATG GAATCTTTCT 
GACATTGATC AAGATGGAAA ACTCACAGCA GAAGAATTTA TCCTGGCTAT GCACCTCATT 
GATGTAGCTA TGTCTGGTCA ACCACTGCCA CCGGTCCTGC CCCCAGAATA CATTCCACCT 
TCCTTTAGAA GAGTTCGATC TGGTAGTGGT ATATCTGTCA TAACCTCAAC TTCGGTGGAT 
CAGAGGTTGC CAGAGGAACC AGTTTTGGAA GATGAACAGC AACAACTAGA AAAGAAACTA 
CCTGTAACCT TTGAAGATAA GAAGCGGGAG AACTTTGAAC GGGGCAACCT GGAGCTGGAG 
AAGCGGAGAC AAGCGCTCTT GGAGCAGCAG CGTAAAGAGC AGGAGCGCCT GGCCCAGCTG 
GAGCGGGCAG AGCAGGAGAG GAAGGAGCGG GAGCGCCAGG AGCAGGAGCG CAAAAGACAA 
CTGGAGCTGG AAAAGCAGCT AGAGAAGCAG CGGGAGCTGG AGCGGCAGAG GGAGGAGGAG 
CGAAGGAAGG AAATCGAGAG GCGGGAGGCT GCGAAACGGG AGCTTGAAAG GCAAAGACAG 
CTTGAATGGG AACGGAATCG AAGACAAGAA CTATTAAATC AAAGAAACAA AGAACAAGAA 
GACATAGTTG TACTGAAAGC AAAGAAAAAG ACTTTGGAAT TTGAATTAGA AGCTCTAAAT 
GATAAAAAAC ATCAATTAGA AGGAAAACTT CAGGATATCA GATGTCGACT GACAACCCAA 
AGGCAAGAAA TTGAGAGCAC AAACAAATCT AGAGAGTTGA GAATTGCAGA AATCACCCAC 
CTACAACAGC AATTACAGGA GTCCCAGCAA ATGCTTGGAA GACTTATTCC AGAAAAACAG 
ATACTCAATG ACCAATTGAA ACAAGTTCAG CAAAACAGTT TGCACAGGGA TTCACTTCTC 
ACACTCAAAA GAGCCTTAGA AGCGAAAGAA CTGGCTCGGC AGCAGCTACG AGACCAACTG 
GATGAAGTGG AGAAAGAAAC TAGGTCAAAA TTACAGGAGA TTGATATTTT CAACAATCAG 
CTGAAGGAAC TTAGAGAAAT ACATAATAAG CAGCAGCTCC AGAAACAGAA GTCCATAGAG 
GCTGAACGAC TGAAACAGAA GGAACAAGAA CGAAAGATCC TGGAATTAGA AAAACAAAAA 
GAAGAAGCCC AAAGACGAGC TCAGGAAAGG GACCAGCAGT GGCTGGAGCG CGTGCATCAG 
GAGGATGAGC AGCAGCGGCC ACGAAAACCC CACGAGGAGG AGAAGCTCAA AAGGGAGGAG 
AGCGTCAAAA AGAAGGACAG TGAGGAGAAG GGCAAACCGG AAATGCAAGA CAAGCTGAGT 
CGGCTTTTCC ACCAGCATCA AGAACCAGCT AAGCCCGCTG TCCAGGCACC CTGGTCCACT 
GCAGAAAAAG GCCCACTTAC GATTTCTGCA CAGGAGAATA TAAAAGTGGT GTATTACCGA 
GCACTTTATC CCTTTGAGTC CAGGAGTCAC GATGAAATCA CAATCCAGCC GGGAGATATA 
GTCATGGTGG ACGAAAGCCA AACTGGAGAA CCGGGATGGC TTGGAGGAGA ATTAAAAGGA 
AAGACGGGAT GGTTCCCTGC AAACTACGCA GAGAAAATCC CAGAAAATGA GGTTCCCGCC 
CCAGTCAAAC CAGTGACTGA GGCGGCCTCC ACCCCTACAC CCAAAGTGGC TGTGTGTGAG 
ACCCCCGCCC CTTCATCCAC AACCCCTGCA GAGCCCTCCG AGACCCCCAA CAACTGGGCC 
GACTTCAGCT CCACGTGGCC CACCAGCACG AATGAGAAAC CAGAAACCGA CAGCTGGGAC 
GCATGGGCAA CCCAGCCCTC CCTCACCGTG CCAAGCGCCG GGCAGCTGAG GCAGAGGTCG 
GCCTTCACTC CAGCCACGGC CACGGGCTCA TCTCCGTCTC CTGTTCTAGG CCAGGGTGAA 
AAGGTGGAGG GGCTACAGGC ACAAGCCCTG TATCCGTGGA GAGCCAAGAA AGACAACCAT 
TTAAATTTTA ACAAAAACGA CGTCATCACC GTACTGGAGC AGCAAGACAT GTGGTGGTTT 
GGAGAAGTTC AAGGCCAGAA GGGTTGGTTC CCCAAGTCCT ACGTGAAACT CATTTCAGGG 
CCCATAAGGA AATCGACAAG CATGGATTCT GGTCCCTCAG AAAGTCCTGC AAGTCTAAAG 
AGGGTAGCTT CCCCAGCAGC CAAGCCAGCC GTGTCGGGAG AAGAATTTAT TGCCATGTAC 
ACTTACGAGA GTTCTGAGCA AGGCGATTTA ACCTTTCAGC AAGGGGATGT GATTTTGGTT 
ACCAAGAAAG ATGGTGACTG GTGGACAGGA ACAGTGGGCG ACAAGTCCGG CGTCTTCCCT 
TCTAACTATG TGAGGCTTAA AGATTCGGAG GGCTCTGGAA CTGCTGGGAA AACAGGGAGT 
TTAGGAAAAA AACCTGAAAT CGCGCAGGTC ATTGCCTCCT ACACAGCTAC GGGTCCTGAA 
CAGCTTACCC TGGCCCCCGG TCAGCTGATC CTGATCCGGA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTACAGGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT TCCTGCTAAT 
TACGTCAAAC TTTTAAGCCC TGGGACAAGC AAAATCACTC CAACAGACCC ACCGAAGCCA 
ACAGCTTTCC CAACAGTGTG CCAGGTGATC GGGATGTACG ACTACACCGC GCAGAACGAC 
GACGAGCTGG CCTTCAGCAA GGGCCAGGTC ATCAATGTCC TCAACAAGGA GGACCCCGAC 
TGGTGGAAGG GTGAAGTCCA CGGGCAGGTG GGGCTCTTCC CGTCCAACTA CGTGAAGTTG 
ACCACGGACA CGGACCCCAG CCAGCAATGG TGCTCAGACT TACACCTCTT GGATATGCTG 
ACCCCAACTG AAAGAAAGCG ACAGGGGTAC ATCCACGAGC TCATCGTCAC GGAGGAGAAC 
TACGTGAATG ACCTGCAGCT GGTCACAGAG ATCTTTCAGA AACCCCTGAT GGAGTCTGAG 
CTGCTGACAG AAAAAGAGGG TGCTATGATT TTTGTTAACT GGAAGGAGCT GATTATGTGT 
AATATCAAAC TACTGAAAGC GCTGAGGGTC CGCAAGAAGA TGTCTGGGGA GAAGATGCCC 
GTGAAGATGA TCGGAGACAT CCTGACGGCC CAGCTGCCAC ACATGCAGCC CTACATCCGC 
TTCTGCAGCT GCCAGCTCAA CGGGGCCGCC TTGATCCAGC AGAAGACTGA CGAGGCCCCA 
GACTTCAAGG AGTTTGTCAA AAGACTGGCC ATGGACCCAC GCTGTAAGGG AATGCCCCTG 
TCTAGTTTTA TACTGAAGCC TATGCAACGA GTAACGAGAT ACCCACTGAT CATTAAAAAT 
ATCCTGGAAA ACACTCCTGA AAACCACCCA GACCACAGCC ACCTGAAGCA CGCCCTGGAG 
AAGGCGGAGG AGCTGTGCTC CCAGGTGAAT GAAGGGGTGC GGGAGAAGGA GAACTCGGAC 
CGACTGGAGT GGATCCAGGC CCACGTGCTG TGTGAAGGCT TGTCAGAGCA ACTTGTGTTC 
AATTCCGTGA CCAACTGCCT GGGGCCGCGC AAGTTCCTGC ACAGTGGGAA GCTCTACAAG 
GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTCTTCAACG ACTTCCTGCT GCTGACCCAA 
ATCATCAAGC CCCTGGGCTC CTCGGGCACC GACAAAGTCT TCAGCCCCAA ATCAAACCTG 
CAGTATAAAA TGTACAAAAC ACCTATTTTC CTAAATGAGG TTCTAGTGAA GTTGCCCACT 
GACCCTTCTG GGGATGAGCC CATCTTCCAC ATTTCCCACA TTGACCGAGT CTACACCCTC 
CGAGCAGAGA GCATAAATGA AAGGACTGCC TGGGTGCAGA AAATCAAAGC AGCGTCTGAA 
CTCTACATAG AGACAGAGAA GAAGAAGCGG GAGAAGGCGT ACCTGGTCCG CTCCCAAAGA 
GCCACAGGCA TCGGAAGGTT GATGGTGAAC GTGGTTGAAG GCATTGAGCT GAAACCCTGT 
CGCTCCCATG GAAAGAGTAA CCCGTACTGT GAGGTGACCA TGGGCTCCCA GTGTCACATC 
ACCAAGACGA TCCAGGACAC TCTGAATCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC 
AGAGACCTGG AGCAGGAGGT GCTGTGCATC ACTGTGTTTG AGAGGGACCA GTTCTCGCCA 
GATGATTTTT TGGGTCGGAC GGAGATCCGC GTGGCCGACA TCAAGAAGGA CCAGGGCTCC 
AAGGGTCCCG TTACGAAGTG TCTCCTGCTG CACGAAGTTC CCACGGGAGA GATCGTGGTC 
CGCTTGGACC TGCAGTTGTT CGATGAGCCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849363.1
CDS	586..5538
Translation	MNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVCETPAPSSTTPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_024993595.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intersectin 1 (ITSN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993595.1

ORF Insert Sequence:

ATGAATAATG ATGGAAGGAT GGATCAAGTG GAGTTTTCCA TTGCTATGAA ACTCATCAAG 
CTGAAGCTCC AAGGATATCA GCTCCCCTCT GCACTTCCCC CTGTCATGAA ACAGCAACCA 
GTTGCTATTT CTAGTGCACC AGCCTTTGGT ATGGGAGGTA TTGCCAGCAT GCCGCCGCTG 
ACCGCTGTTG CTCCTGTACC AATGGGCTCC ATTCCCGTTG TTGGGATGTC TCCCCCACTA 
GTGTCATCTG TTCCTGCAGC AGGGGTGCCC CCCCTGGCCA ATGGGGCGCC CCCGGTCATA 
CAGCCTCTGC CTGCATTTGC TCATCCTGCT GCCACATTGC CAAAGAGTTC TTCCTTTAGT 
AGATCTGGCC CAGGGTCGCA GTTAAACACA AAATTACAGA AGGCACAATC ATTTGATGTG 
GCCAGTGTCC CTCCTACAGC TGAGTGGGCT GTTCCTCAGT CATCCAGGCT GAAATACAGA 
CAGCTCTTCA ATAGTCATGA CAAAACCATG AGTGGACACC TAACAGGTCC CCAAGCAAGA 
ACTATTCTTA TGCAATCAAG TTTACCACAG GCTCAGCTGG CTTCAATATG GAATCTTTCT 
GACATTGATC AAGATGGAAA ACTCACAGCA GAAGAATTTA TCCTGGCTAT GCACCTCATT 
GATGTAGCTA TGTCTGGTCA ACCACTGCCA CCGGTCCTGC CCCCAGAATA CATTCCACCT 
TCCTTTAGAA GAGTTCGATC TGGTAGTGGT ATATCTGTCA TAACCTCAAC TTCGGTGGAT 
CAGAGGTTGC CAGAGGAACC AGTTTTGGAA GATGAACAGC AACAACTAGA AAAGAAACTA 
CCTGTAACCT TTGAAGATAA GAAGCGGGAG AACTTTGAAC GGGGCAACCT GGAGCTGGAG 
AAGCGGAGAC AAGCGCTCTT GGAGCAGCAG CGTAAAGAGC AGGAGCGCCT GGCCCAGCTG 
GAGCGGGCAG AGCAGGAGAG GAAGGAGCGG GAGCGCCAGG AGCAGGAGCG CAAAAGACAA 
CTGGAGCTGG AAAAGCAGCT AGAGAAGCAG CGGGAGCTGG AGCGGCAGAG GGAGGAGGAG 
CGAAGGAAGG AAATCGAGAG GCGGGAGGCT GCGAAACGGG AGCTTGAAAG GCAAAGACAG 
CTTGAATGGG AACGGAATCG AAGACAAGAA CTATTAAATC AAAGAAACAA AGAACAAGAA 
GACATAGTTG TACTGAAAGC AAAGAAAAAG ACTTTGGAAT TTGAATTAGA AGCTCTAAAT 
GATAAAAAAC ATCAATTAGA AGGAAAACTT CAGGATATCA GATGTCGACT GACAACCCAA 
AGGCAAGAAA TTGAGAGCAC AAACAAATCT AGAGAGTTGA GAATTGCAGA AATCACCCAC 
CTACAACAGC AATTACAGGA GTCCCAGCAA ATGCTTGGAA GACTTATTCC AGAAAAACAG 
ATACTCAATG ACCAATTGAA ACAAGTTCAG CAAAACAGTT TGCACAGGGA TTCACTTCTC 
ACACTCAAAA GAGCCTTAGA AGCGAAAGAA CTGGCTCGGC AGCAGCTACG AGACCAACTG 
GATGAAGTGG AGAAAGAAAC TAGGTCAAAA TTACAGGAGA TTGATATTTT CAACAATCAG 
CTGAAGGAAC TTAGAGAAAT ACATAATAAG CAGCAGCTCC AGAAACAGAA GTCCATAGAG 
GCTGAACGAC TGAAACAGAA GGAACAAGAA CGAAAGATCC TGGAATTAGA AAAACAAAAA 
GAAGAAGCCC AAAGACGAGC TCAGGAAAGG GACCAGCAGT GGCTGGAGCG CGTGCATCAG 
GAGGATGAGC AGCAGCGGCC ACGAAAACCC CACGAGGAGG AGAAGCTCAA AAGGGAGGAG 
AGCGTCAAAA AGAAGGACAG TGAGGAGAAG GGCAAACCGG AAATGCAAGA CAAGCTGAGT 
CGGCTTTTCC ACCAGCATCA AGAACCAGCT AAGCCCGCTG TCCAGGCACC CTGGTCCACT 
GCAGAAAAAG GCCCACTTAC GATTTCTGCA CAGGAGAATA TAAAAGTGGT GTATTACCGA 
GCACTTTATC CCTTTGAGTC CAGGAGTCAC GATGAAATCA CAATCCAGCC GGGAGATATA 
GTCATGGTGG ACGAAAGCCA AACTGGAGAA CCGGGATGGC TTGGAGGAGA ATTAAAAGGA 
AAGACGGGAT GGTTCCCTGC AAACTACGCA GAGAAAATCC CAGAAAATGA GGTTCCCGCC 
CCAGTCAAAC CAGTGACTGA GGCGGCCTCC ACCCCTACAC CCAAAGTGGC TGTGTGTGAG 
ACCCCCGCCC CTTCATCCAC AACCCCTGCA GAGCCCTCCG AGACCCCCAA CAACTGGGCC 
GACTTCAGCT CCACGTGGCC CACCAGCACG AATGAGAAAC CAGAAACCGA CAGCTGGGAC 
GCATGGGCAA CCCAGCCCTC CCTCACCGTG CCAAGCGCCG GGCAGCTGAG GCAGAGGTCG 
GCCTTCACTC CAGCCACGGC CACGGGCTCA TCTCCGTCTC CTGTTCTAGG CCAGGGTGAA 
AAGGTGGAGG GGCTACAGGC ACAAGCCCTG TATCCGTGGA GAGCCAAGAA AGACAACCAT 
TTAAATTTTA ACAAAAACGA CGTCATCACC GTACTGGAGC AGCAAGACAT GTGGTGGTTT 
GGAGAAGTTC AAGGCCAGAA GGGTTGGTTC CCCAAGTCCT ACGTGAAACT CATTTCAGGG 
CCCATAAGGA AATCGACAAG CATGGATTCT GGTCCCTCAG AAAGTCCTGC AAGTCTAAAG 
AGGGTAGCTT CCCCAGCAGC CAAGCCAGCC GTGTCGGGAG AAGAATTTAT TGCCATGTAC 
ACTTACGAGA GTTCTGAGCA AGGCGATTTA ACCTTTCAGC AAGGGGATGT GATTTTGGTT 
ACCAAGAAAG ATGGTGACTG GTGGACAGGA ACAGTGGGCG ACAAGTCCGG CGTCTTCCCT 
TCTAACTATG TGAGGCTTAA AGATTCGGAG GGCTCTGGAA CTGCTGGGAA AACAGGGAGT 
TTAGGAAAAA AACCTGAAAT CGCGCAGGTC ATTGCCTCCT ACACAGCTAC GGGTCCTGAA 
CAGCTTACCC TGGCCCCCGG TCAGCTGATC CTGATCCGGA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTACAGGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT TCCTGCTAAT 
TACGTCAAAC TTTTAAGCCC TGGGACAAGC AAAATCACTC CAACAGACCC ACCGAAGCCA 
ACAGCTTTCC CAACAGTGTG CCAGGTGATC GGGATGTACG ACTACACCGC GCAGAACGAC 
GACGAGCTGG CCTTCAGCAA GGGCCAGGTC ATCAATGTCC TCAACAAGGA GGACCCCGAC 
TGGTGGAAGG GTGAAGTCCA CGGGCAGGTG GGGCTCTTCC CGTCCAACTA CGTGAAGTTG 
ACCACGGACA CGGACCCCAG CCAGCAATGG TGCTCAGACT TACACCTCTT GGATATGCTG 
ACCCCAACTG AAAGAAAGCG ACAGGGGTAC ATCCACGAGC TCATCGTCAC GGAGGAGAAC 
TACGTGAATG ACCTGCAGCT GGTCACAGAG ATCTTTCAGA AACCCCTGAT GGAGTCTGAG 
CTGCTGACAG AAAAAGAGGG TGCTATGATT TTTGTTAACT GGAAGGAGCT GATTATGTGT 
AATATCAAAC TACTGAAAGC GCTGAGGGTC CGCAAGAAGA TGTCTGGGGA GAAGATGCCC 
GTGAAGATGA TCGGAGACAT CCTGACGGCC CAGCTGCCAC ACATGCAGCC CTACATCCGC 
TTCTGCAGCT GCCAGCTCAA CGGGGCCGCC TTGATCCAGC AGAAGACTGA CGAGGCCCCA 
GACTTCAAGG AGTTTGTCAA AAGACTGGCC ATGGACCCAC GCTGTAAGGG AATGCCCCTG 
TCTAGTTTTA TACTGAAGCC TATGCAACGA GTAACGAGAT ACCCACTGAT CATTAAAAAT 
ATCCTGGAAA ACACTCCTGA AAACCACCCA GACCACAGCC ACCTGAAGCA CGCCCTGGAG 
AAGGCGGAGG AGCTGTGCTC CCAGGTGAAT GAAGGGGTGC GGGAGAAGGA GAACTCGGAC 
CGACTGGAGT GGATCCAGGC CCACGTGCTG TGTGAAGGCT TGTCAGAGCA ACTTGTGTTC 
AATTCCGTGA CCAACTGCCT GGGGCCGCGC AAGTTCCTGC ACAGTGGGAA GCTCTACAAG 
GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTCTTCAACG ACTTCCTGCT GCTGACCCAA 
ATCATCAAGC CCCTGGGCTC CTCGGGCACC GACAAAGTCT TCAGCCCCAA ATCAAACCTG 
CAGTATAAAA TGTACAAAAC ACCTATTTTC CTAAATGAGG TTCTAGTGAA GTTGCCCACT 
GACCCTTCTG GGGATGAGCC CATCTTCCAC ATTTCCCACA TTGACCGAGT CTACACCCTC 
CGAGCAGAGA GCATAAATGA AAGGACTGCC TGGGTGCAGA AAATCAAAGC AGCGTCTGAA 
CTCTACATAG AGACAGAGAA GAAGAAGCGG GAGAAGGCGT ACCTGGTCCG CTCCCAAAGA 
GCCACAGGCA TCGGAAGGTT GATGGTGAAC GTGGTTGAAG GCATTGAGCT GAAACCCTGT 
CGCTCCCATG GAAAGAGTAA CCCGTACTGT GAGGTGACCA TGGGCTCCCA GTGTCACATC 
ACCAAGACGA TCCAGGACAC TCTGAATCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC 
AGAGACCTGG AGCAGGAGGT GCTGTGCATC ACTGTGTTTG AGAGGGACCA GTTCTCGCCA 
GATGATTTTT TGGGTCGGAC GGAGATCCGC GTGGCCGACA TCAAGAAGGA CCAGGGCTCC 
AAGGGTCCCG TTACGAAGTG TCTCCTGCTG CACGAAGTTC CCACGGGAGA GATCGTGGTC 
CGCTTGGACC TGCAGTTGTT CGATGAGCCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_015465187.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320673.1 Bos taurus intersectin 1 (ITSN1), mRNA.

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CloneID	OBa87631
Clone ID Related Accession (Same CDS sequence)	XM_015465187.1 , XM_015461917.1
Accession Version	XM_015465187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: intersectin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTCNAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGTGT GAGACCCCCG CCCCTTCATC CACAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCACAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGACGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTCCCT CAGAAAGTCC TGCAAGTCTA AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCCGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCG 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCGCAG 
GTCATTGCCT CCTACACAGC TACGGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACA 
AGCAAAATCA CTCCAACAGA CCCACCGAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG TTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGCTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGACAGGGG 
TACATCCACG AGCTCATCGT CACGGAGGAG AACTACGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT CAAAAGACTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC TGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCGG AGGAGCTGTG CTCCCAGGTG 
AATGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTG 
CTGTGTGAAG GCTTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ACGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCGGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATT 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA GCCCATCTTC 
CACATTTCCC ACATTGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA TGAAAGGACT 
GCCTGGGTGC AGAAAATCAA AGCAGCGTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTACCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGAAAGAG TAACCCGTAC 
TGTGAGGTGA CCATGGGCTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACC TGGAGCAGGA GGTGCTGTGC 
ATCACTGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CCGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320673.1
CDS	288..5453
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFnullSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVCETPAPSSTTPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_015465187.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intersectin 1 (ITSN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465187.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTCNAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGTGT GAGACCCCCG CCCCTTCATC CACAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCACAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGACGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTCCCT CAGAAAGTCC TGCAAGTCTA AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCCGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCG 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCGCAG 
GTCATTGCCT CCTACACAGC TACGGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACA 
AGCAAAATCA CTCCAACAGA CCCACCGAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG TTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGCTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGACAGGGG 
TACATCCACG AGCTCATCGT CACGGAGGAG AACTACGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT CAAAAGACTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC TGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCGG AGGAGCTGTG CTCCCAGGTG 
AATGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTG 
CTGTGTGAAG GCTTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ACGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCGGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATT 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA GCCCATCTTC 
CACATTTCCC ACATTGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA TGAAAGGACT 
GCCTGGGTGC AGAAAATCAA AGCAGCGTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTACCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGAAAGAG TAACCCGTAC 
TGTGAGGTGA CCATGGGCTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACC TGGAGCAGGA GGTGCTGTGC 
ATCACTGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CCGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_015461917.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317403.1 Bos taurus intersectin 1 (ITSN1), mRNA.

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CloneID	OBa87631
Clone ID Related Accession (Same CDS sequence)	XM_015465187.1 , XM_015461917.1
Accession Version	XM_015461917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: intersectin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644478.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTCNAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGTGT GAGACCCCCG CCCCTTCATC CACAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCACAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGACGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTCCCT CAGAAAGTCC TGCAAGTCTA AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCCGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCG 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCGCAG 
GTCATTGCCT CCTACACAGC TACGGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACA 
AGCAAAATCA CTCCAACAGA CCCACCGAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG TTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGCTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGACAGGGG 
TACATCCACG AGCTCATCGT CACGGAGGAG AACTACGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT CAAAAGACTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC TGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCGG AGGAGCTGTG CTCCCAGGTG 
AATGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTG 
CTGTGTGAAG GCTTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ACGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCGGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATT 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA GCCCATCTTC 
CACATTTCCC ACATTGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA TGAAAGGACT 
GCCTGGGTGC AGAAAATCAA AGCAGCGTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTACCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGAAAGAG TAACCCGTAC 
TGTGAGGTGA CCATGGGCTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACC TGGAGCAGGA GGTGCTGTGC 
ATCACTGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CCGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317403.1
CDS	288..5453
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFnullSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERVHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVCETPAPSSTTPAEPSETPNNWADFSSTWPTSTNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_015461917.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intersectin 1 (ITSN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461917.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTCNAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA 
ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA 
GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC 
TCCACCCCTA CACCCAAAGT GGCTGTGTGT GAGACCCCCG CCCCTTCATC CACAACCCCT 
GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC 
GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC 
TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCACAAGCC 
CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGACGTCATC 
ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG 
TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT 
TCTGGTCCCT CAGAAAGTCC TGCAAGTCTA AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA 
GCCGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCG 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCGCAG 
GTCATTGCCT CCTACACAGC TACGGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG 
ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACA 
AGCAAAATCA CTCCAACAGA CCCACCGAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG 
ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG 
GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG 
GTGGGGCTCT TCCCGTCCAA CTACGTGAAG TTGACCACGG ACACGGACCC CAGCCAGCAA 
TGGTGCTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGACAGGGG 
TACATCCACG AGCTCATCGT CACGGAGGAG AACTACGTGA ATGACCTGCA GCTGGTCACA 
GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG 
ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG 
GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG 
GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC 
GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT CAAAAGACTG 
GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA 
CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC TGAAAACCAC 
CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCGG AGGAGCTGTG CTCCCAGGTG 
AATGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTG 
CTGTGTGAAG GCTTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG 
CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTCTTCA ACGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCGGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATT 
TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA GCCCATCTTC 
CACATTTCCC ACATTGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA TGAAAGGACT 
GCCTGGGTGC AGAAAATCAA AGCAGCGTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG 
CGGGAGAAGG CGTACCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGAAAGAG TAACCCGTAC 
TGTGAGGTGA CCATGGGCTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACC TGGAGCAGGA GGTGCTGTGC 
ATCACTGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CCGTTACGAA GTGTCTCCTG 
CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ITSN1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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