COL7A1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL7A1
Entrez Gene ID	507133
Full Name	collagen type VII alpha 1 chain
General protein information	Preferred Names collagen type VII alpha 1 chain Names collagen alpha-1(VII) chain collagen, type VII, alpha 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002697051.6	XP_002697097.2	collagen alpha-1(VII) chain
XM_002697051.5	XP_002697097.2	collagen alpha-1(VII) chain
XM_015468790.1	XP_015324276.1	collagen alpha-1(VII) chain

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col7a1	NP_001100328.1
Bos taurus (cattle)	COL7A1	XP_002697097.2
Mus musculus (house mouse)	Col7a1	NP_031764.2
Gallus gallus (chicken)	COL7A1	XP_425157.4
Xenopus tropicalis (tropical clawed frog)	col7a1	XP_002933125.2
Canis lupus familiaris (dog)	COL7A1	NP_001002980.1
Danio rerio (zebrafish)	col7a1	XP_002662551.3
Homo sapiens (human)	COL7A1	NP_000085.1
Pan troglodytes (chimpanzee)	COL7A1	XP_003950149.1

Gene Ontology

The following COL7A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL7A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa229323	XM_002697051.6 Latest version!	Bos taurus collagen type VII alpha 1 chain (COL7A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa31702	XM_015468790.1 Latest version!	Bos taurus collagen type VII alpha 1 (COL7A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa31702	XM_002697051.5 Latest version!	Bos taurus collagen type VII alpha 1 (COL7A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL7A1 ( XM_002697051.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697097.2 Bos taurus collagen type VII alpha 1 chain (COL7A1), mRNA.

Price & Availability↑

CloneID	OBa229323
Clone ID Related Accession (Same CDS sequence)	XM_002697051.6
Accession Version	XM_002697051.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	collagen alpha-1(VII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697051.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTGC GGCTCCTGGT GGCTGCGCTC TGCGCCGGGA TCCTGGCAGG GGCTCCCCGA 
GTGTGGGCCC AGCCCAGGGA GAGAGTGAGC TGCACGCGCC TCTACGCCGC TGACATCGTG 
TTCCTCCTTG ACGGCTCCTC GTCCATCGGC CGAAGCAACT TCCGCGAAGT GCGGGGTTTC 
CTTGAGGGGC TGGTGCTGCC CTTCTCCGGG GCAGCTGGGG CACAGGGTGT GCGCTTCGCG 
GCCGTGCAGT ACAGCGACGA CCCACGGACA GAATTTGACC TGGATGCACT TGGCTCAGGC 
GGAGATGTGA TCCGTGCCAT CCGAGAGCTC AGCTACAAGG GGGGCAACAC GCGCACAGGG 
GCCGCGATTC TCCACGTGGC TGACCGCGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GTATCCTCAT CACAGACGGG AAGTCCCAGG ACATGGTGGA CACAGCTGCC 
CAGAGGTTGA AGGGGCAGGG AGTCAAGCTG TTTGCTGTGG GCATCAAGAA TGCCGACCCC 
GAGGAGCTGA AGCGAATTGC CTCACAGCCG ACTAGCGATT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACGCT CCTGCCTCTG GTTTCCCGGA GGGTGTGCAC AACTGCGGGT 
GGTGTGCCCG TGACCCTGCC CCCCGACGAC TCGACCTCTG GTCCACGAGA CCTGCTGCTT 
GAGCCAGGCA GTCAGTCCTT GAGAGTCCAG TGGACAGCGG CCAGTGGCCC CGTGACTGGC 
TACAAGGTCC AGTACGCACC CCTGACAGGG CTGGGACAGC CATTGCCAAG CGAGCTGCGG 
GAGGTGAGCG TCCCAGCTGG TGAGACCAGC GTGCAGCTGC AGGGTCTCCG GCCACTGACT 
GAGTACCAAG TGACGGTGGT CGCCCTCTAC GCCAACAGCA TCGGGGAGGC CGTGAGCGGG 
ACCGCTAGGA CCACTGCTCT GGAGGGGCCA GAAGTGACCA TCCAGAACAC CACAGCCCAC 
AGCCTCCTGG TGGCCTGGCA GAGTGTGCCA GGTGCCACTG GCTACCGGGT GACGTGGCGG 
GTCGTCAGGG GTGGGGCCAC ACAGCAGCAG GAGCTGGGCT CTGGGCAGGG GTCAGTGTTG 
CTGAGGGACC TGCAGCCTGG CACCGACTAC GAGGTGACCG TGAGTGCCCT GCTTGGCCGG 
AGCGTGGGGC CAGCCACCTC CCTGACTGCC CGCACTGACA CGTCCGTCGA GCAGACCCTG 
CGTCCTATCA TCCTGGGCCC CACGTCCATC CTCCTCTCCT GGAACCTGGT GCCTGAGGCC 
CGTGGCTACC GGCTGGAATG GCGGCGTGAG AGCGGCTTAG AGGTGCCACA GAAGGTGGTG 
CTGCCCTCTG ACGTGACCCG CTACCAGTTG GATGGGCTGC AGCCAGGCAC CGAGTACCGC 
CTCACTCTCT ACACGCTGCT AGAGGGCCGT GAGGTGGCCA CCCCAGCGAC CGTGGTCCCC 
ACTGAGCTGC CCGTGGGCCC CGTCACAGCC CTCCAGGCCA CCGAGCTGCC TGGGCAGCGT 
GTGCGAGTGT CCTGGAGCCC AGTTCCCGGC GCCACCGAGT ATCGCATCAC TGTGCGCAGC 
GCCCAGGGGG TTGAGCGGAG CCTGGTGCTT CCTGGGAGTC AGACGGCCTT TGACTTGGAT 
GACGTCCGGG CTGGGCTCAG CTACACAGTG CGGGTGTCAG CGCGAGTAGG CGGCCGGGAG 
GGCGCTGCCA GCATCCTCAC TGTCCGCCGG GAGCCAGAAC CCCCACTGGC CATCCCAGGG 
CTGCGGGTCA TGGCATCGGA CGCAACGCGA GTGAGGGTGG CCTGGGGACC AGTTCCCGGA 
GCCAGCGGAT TTCGGATTAG CTGGAGGACA GACAATGGTC TGGAGTCCAG CCAGACAGTT 
CCTTCAGACT CTACTGCCAC CGACATTGTG GGGCTGAGAC CTGGGACCTC CTACCAGGTG 
GCCGTGTCGG CGCTGCGAGG AAGAGAGGAG GGCCCTCCTG CGGTCATCGT GGCTCGAACC 
GATCCCTTGG GCCCAGTGAG ATCGGTCCAT GTGACTCGAG TCAGCAGCTC ATCTGTCACC 
ATCACCTGGA ACCGGGTTCC TGGTGCCACA GGGTACAGGG TCTCCTGGCG CTCAGGCCGA 
GGCCCAGAGA AATCCCAGTT GGTTTCTGGG GAGGCCACGG TGGCCGAGCT GGATGGACTG 
GAGCCAGATA CTGAGTACAC CGTGCGTGTG TGGGCCCGTG TGGCTGGTGT GGACGGGACG 
CCTGCCTCTG CGGTTGTGAG AACTGACCCT CGGCCCGTGG GCAGTGTGTC GAAGCTGCAG 
ATCCATAATG CTTCCAGTGA CGTTCTGCGG GTCACCTGGG TGGGGGTCCC TGGAGCCACA 
GCCTACAGAC TGGCCTGGGG CCGGAGTGAG GGTGGCCCCA CAAGACAGCA GATTCTCCCA 
GGAAACACGG ACTCCGCAGA AATACGGGGC CTCGAAGGCG GAGTCAGTTA TTCAGTGAGA 
GTGACCGCAC TCATAGGGGA CCGCGAGGGC GCGCCTGTCT CCATTGTCGT CACCACCCCT 
CCGGAGGCTC CTCCAGCCCT GGAGACACTT CGCGTGGAGC AGCGAGGGGA GCACTCACTG 
AGGCTGCACT GGCAGCCGGT GCCTGGTGCA CGCGGCTTCC GTCTGCGTTG GCGACCTGAG 
GGTGGCCAGG AACAGTCCCG AGTCCTGGGG CCGGAAGTCA GCAGCTATGA CCTGGATGGG 
CTGGAGCCAG CGACCCACTA CCGCATATGG CTGAGCGTCT TGGGGACGGC TGGAGAAGGG 
CCCCCCTCGG AAGTGACTGC ATACACTGAG TCACCTCGTG TCCCAAGCAC TCAACTGCGT 
GTGGTGGACA CGTCAGTCGA CTCAGTGACT CTGGCCTGGA CCCCAGTGTC TGGGGTATCC 
AGCTACATCC TATCCTGGCG GCCTCTCAGA GGCCCTGGCC AAGAACTGCC TGGGGCCTCA 
CAGACACTTC CGGGGACCTC AAGTTCCCAG CGGGTGACAG GCCTAGAGCT GGGCATCTCC 
TACCTCTTCT CCCTAACTCC TGTCCGGGAC GGTGTACGCG GTCCTGAAGC CTCTATCACA 
CAGAGCCCAG CGTGTCCCCG CGGCCTGATG GATGTGGTGT TCCTGGTGCA CGCCACTCGA 
GACAATGCTC ATCGCTCGGA GGCTGTGAAG CGAGCCCTGG AGCACCTGGT GTCTGCACTC 
GGGCCTCTCG GGCCACAGGC CGTCCAGGTC GGCCTCCTGT CCTACAGTCA CCGGCCCTCC 
CCACTGTTGT CGCTGAACAG CTCCCATGAC CTTGGCGTCA TCCTGCAGAA GATCCGCAAC 
ATCCCCTACA CGGACCCAAG TGGGAACAAC CTGGGCACAG CCGTGGTTAC AGCCCACAGA 
TACCTGCTGG CACCAGATGC GCCTGGACGC CGCCGGCATG TGCCGGGGGT GATGGTTCTC 
CTGGTGGATG AGCCCTTGAG AGGTGACATC TTCAACTCTA TCCGTGAGGC CCAGGCTGCT 
GGGCTCAGAG TGATGATGCT GGGGCTGGCG GGAGCTGACC CAGAGCAGCT GCGTCGCTTG 
GTGCCTGGCA TGGACCCTTC CCAGACCTTC TTCGCTGTGG ATGATGGTCT GAGCTTGGAG 
CGGGCCGTCA GTAGTCTGGC AGCAGCCCTG TGTCAGACAG CCTTGACCAC GCAGCCACAG 
CCAGAGCCTT GCTCAGTGCA TTGTCCAAAG GGCCAGAAGG GGGAACCCGG ACAGATGGGC 
CTGAAAGGAC AAGCCGGGCC TCCTGGCCCC CCCGGCCTCC CGGGCAGGAT TGGTGCTCCT 
GGCCCCCAGG GACCTCCTGG AAGTGCTTCT GCGAAGGGCG AGAGGGGCTT CCCTGGGGCA 
GATGGGCCTC CAGGCAGCCC TGGCCGCCCT GGGACTCCTG GAGCCCCTGG TCCGAAGGGC 
TCCCCAGGGT GGGCCGGCCC TCGTGGAGAA CCAGGAGAGC GAGGACCTCG AGGCCCAAAG 
GGGGAGCCGG GAGAGCCTGG ACAAGTCATT GGAGGCGAGG GCCCAGGGCT TCCTGGGCAG 
AAAGGGGACC CTGGACCTCC AGGTCCCCCT GGATCTCGTG GGCCGTTGGG GGACCCGGGA 
CCCCGGGGTC CCCCGGGATT TCCTGGAACA GCTGTGAAGG GTGAGAAAGG CGATCGTGGG 
GAGCAGGGCC CCCCTGGACC AGGTGACGGC ACTGCTGCTC TGGGCGAGCC TGGGCTGCCG 
GGTCTTCCTG GAAGCCCCGG ACCCCAAGGC TCAGTTGGCC CCCCTGGAGA GAAAGGAGAA 
AAGGGTGACT GTGAAGATGG AGCCCCGGGC CTCCCAGGAC AACCCGGAAT TCCGGGTGAG 
CGGGGCCTTC GGGGACTTCC TGGAGATGCT GGCCCCAAAG GCGACCGAGG ACTAACAGGG 
GCCGCTGGAG AGAAGGGTGA ACGTGGATCG CCTGGCCCAG TGGGACCACA GGGGCTGCCA 
GGAGTTGCTG GACGACCTGG GCCCGAGGGT CCTGAAGGGC CACCAGGACC GACCGGCCGC 
CGAGGAGAGA AGGGGGAGCC TGGTCGGCCT GGAGACCCTG CAGTGGGACC TGGGGGTGCT 
GGAGCTAAAG GAGAGAAGGG AGATATGGGC CCCCCCGGGC CCAAAGGAGC TACCGGATTC 
AAAGGGGAAC AGGGCTCACC TGGCTTGGCT CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC 
CCTGGAGGCC GAGGTCCCAT TGGCCTCACT GGCAGAGCAG GACCCCCGGG CGACTCAGGA 
CCCCCTGGAG AGAAGGGAGA CCCTGGGCGG CCCGGTTCCC CAGGACCCGT CGGCCCCCGA 
GGACGAGACG GTGAAGTTGG AGAGAAGGGC GATGACGGTC CCCCGGGTGA CCCAGGTTTG 
CCTGGAAAAG CTGGCGAGCG TGGCCTTCGG GGAGCGCCTG GAGCTCGGGG GCCTGTGGGT 
GAGAAGGGAG ACCAGGGAGA TCCTGGAGAG GAAGGACGAA ATGGGAGCCC TGGACCGTCT 
GGACCCAAGG GTGACCGTGG GGAGCCAGGT CCCCCAGGAG CCCCTGGACG GCTGGTGGAC 
GTGGGACTTG GAGCCGGAGA GAAGGGAGAG CCTGGGGACC GAGGACAGGA GGGTCCACGA 
GGACCCAAGG GCGACCCTGG CCCCCCTGGA GCTTCTGGGG AGAGGGGCGT CAGTGGACTT 
CGGGGGCCTC CAGGCCCACA GGGGGACCCA GGTGTTCGAG GCCCAGCAGG AGAAAAGGGC 
GACCGGGGCC CTCCTGGCCT GGATGGCCGC AGTGGGCTGG ATGGGAAACC GGGAGCCTCT 
GGTCCCCCTG GGCCGAATGG TGCCATGGGC AAGGCCGGAG ACCCAGGGAG AGACGGACTT 
CCGGGCCTTC GAGGAGAACA GGGCCCCCCT GGCCCTGCTG GTCCCCCTGG AGCACCGGGA 
AAGCCGGGCG AGGATGGCAA GCCTGGCCTG AATGGGAAAA ACGGAGAACC CGGGGACCCT 
GGAGAAGACG GGAGGAAGGG AGAGAAGGGA GATTCAGGCG CCCCTGGGAG AGAAGGTCGT 
GACGGCCCCA AGGGTGAGCG TGGAGCTCCT GGTAGCCCTG GACTCCAGGG CCCCCCGGGC 
CTCCCAGGGC AGGTCGGCCC TCCCGGGCAG GGCTTCCCTG GCGTCCCGGG GAGTTCAGGC 
CCCAAGGGTG ACCGTGGGGA GACTGGACCC AGAGGGGAGC AGGGCCTCCC AGGAGAGCGT 
GGCCTGAGAG GAGAGCCTGG GAGCCTGGTG AATGCAGAGC GGTTGCTGGA AAATATTGGC 
ATCAAGACAT CTGCCCTGAG GGAGATCGTG GAGACCTGGG AAGAAAGCTC TGGTGGCTTC 
CTGCCCACGC CTGAACGGCG TCGCGGCCCC AAGGGGGACC CAGGCGAGCG GGGCCCCCCA 
GGCAAGGAGG GCCCCATCGG CTTTTCTGGA GAACGTGGAC CAAAGGGAGA TCGTGGAGAC 
CCCGGCCCTC AGGGGCCACC TGGCCTGGCC CTGGGGGAGA GGGGCCTTCC TGGACCTACC 
GGCCTTGCCG GGGAGCCTGG GAAGCCTGGT ATCCCTGGGC TCCCTGGCCC AGCTGGGAGT 
GTGGGGGAGG CCGGAAGACC CGGAGAGAGG GGAGAACGGG GAGAGAAAGG AGAACGTGGA 
GAACAGGGCA GAGACGGCCT TCCTGGCCTC CCTGGACCCC CCGGACCCCC AGGCCCCAAG 
GTGGCCGTGG ACGAGCCAGG TTCTGGGCTC TCTAGAGAAC AAGGACCCCC TGGATTCAAG 
GGTGCTAAGG GGGATCCAGG CAGTGATGGC GACCGAGGCC CCAAAGGAGA CAGGGGTGAG 
CCGGGCATCA AGGGCGACCG GGGAGAGCCT GGACAGAGGG GTCCGAATGG CAGCCCGGGT 
CTGCCAGGAG AGCGCGGCGT GGCTGGGCCC GAGGGGAAGC CGGGTCTGCA AGGTCCACGG 
GGGACCCCTG GCCCGGCGGG CGGCCATGGA GACCCTGGAC CACCCGGGGC CCCGGGTCTT 
GCTGGCCCTG CGGGACCCCA GGGACCTTCT GGCCTAAAGG GGGAGCCCGG AGAGACAGGA 
CCACCAGGAC GGGGCCTGCC TGGACCTACT GGAGCCGTGG GACTTCCCGG GCCCCCTGGC 
CCTTCAGGCC TGGTGGGTCC TCAAGGGGCA CCGGGTTTGC CTGGACAAGT GGGGGAGACG 
GGAAAGCCGG GAGCGCCAGG TCGTGATGGT GCCACTGGGA AAGATGGAGA CAGAGGAGCC 
CCCGGTGTGC CGGGGTCACC AGGTCTGCCA GGCCCTGTCG GACCTAAAGG AGAGCCGGGA 
CCCATGGGGA CCCCTGGACA GGCTGTGGTC GGGCCCCCTG GAGCAAAGGG AGAGAAGGGT 
GCCCCTGGAG CCCTTGCTGG AGACCTGGTG GGAGAGCCGG GAGCCAAGGG TGACCGAGGA 
CTGCCAGGGC CGCGGGGCGA GAAGGGTGAA GCTGGCCGTG CGGGGGAGCC TGGAGACCCT 
GGCGAAGATG GTCAGAAGGG GGCTCCGGGA CCCAAAGGTC ACAAGGGTGA CCCAGGAGTT 
GGGGTCCAGG GGCCCCCTGG GCCAGTTGGC CCTCCAGGTC TAAAGGGAGA CTCAGGGCCC 
CCCGGATCCC CCGGACCTCC TGGTATTGTG GGGTTCCCCG GTCAGACAGG CCCTCGAGGA 
GAGCTGGGGC AGCCGGGCCC CAGTGGAGAG CGGGGTCTGG CAGGTGCCCC AGGGAGAGAG 
GGAGCCCCAG GTCCCTTGGG GCCGCCTGGA CCACCTGGGT CAGCGGGAGT ACCTGGGGTC 
CCTGGACTCA AAGGAGACAA GGGTGACACT GGAGCAGGGC TGCCTGGGGC CCGAGGCGAG 
CGTGGGGAGC CCGGTGTCCG GGGTGAAGAT GGCCGCCCCG GCCTGGAGGG ACCCCGAGGA 
CTTGCGGGTC CCCCAGGCAT CCGGGGAGAA CGTGGGGAGA AGGGTGACGC TGGTGCCTCA 
GGACTGAAGG GTGACAAGGG CGACTCAGCT GTGATTTTGG GGCCTCCGGG GCCACGGGGT 
GCCAAGGGGG ACTCGGGTGA ACGAGGGCCT CGGGGAATAG ATGGTGACAA AGGACCTCGG 
GGAGACAGCG GGGAACCTGG AGAGAAGGGC ACCAAGGGAG AGCCTGGTGA CAAGGGCTCA 
GCCGGGCTGC TGGGAGCGCG CGGACTCATG GGACCCAAGG GCGAGCCTGG TGCCGCAGGG 
ATCCCCGGCG AACCGGGATC CCCAGGAAAA GACGGAGTCC CTGGTGTCCG AGGAGACAAA 
GGAGATGTTG GCTTCATGGG TCCCCGGGGC CTCAAGGGTG AACGGGGAAT TAAGGGAGCC 
TGTGGCCTCG ACGGAGAGAA GGGAGATAAG GGAGAAGCCG GCCCCCCGGG CCGCCCAGGG 
CTGGCAGGAC GCAGAGGAGA GCTGGGGGAG CCGGGTGTTC CAGGCCAGGC AGGGGCCCCT 
GGGAAGGAGG GCCTGATCGG TCCCAAGGGT GACCGAGGTT TCGATGGGCA GCCAGGACCC 
AAGGGTGACC AGGGCGAGAA AGGGGAGCGG GGACCCCCAG GAGTGGGGGG CTTCCCAGGT 
CCCAGGGGCA GTGATGGCTC TAGTGGTCCT CCCGGGCCAC CTGGCAGCAT TGGTCCCAAA 
GGCCCTGAAG GACTTCAGGG TCAGAAGGGT GAGCGAGGTC CCCCTGGAGA GAGCGTGGTG 
GGTGCCCCTG GGGCCCCTGG AACTCCTGGA GAGAGAGGGG AGCAGGGGCG ACCAGGCCCC 
GCGGGGCCCC GTGGTGAAAA GGGAGAAGCT GCGCTGACGG AGGATGACAT TCGGGGCCTT 
GTGCGTCAGG AGATGAGTCA ACATTGCGCC TGTCAGGGCC AGTATATCGC ATCTGGATCA 
CGGCCTCTCC CGAGTTACGC AGCAGACACC GCTGGCCCGC AGCTCCACCC CGTGCCCGTG 
CTCCGCGTGT CCCACGCAGA GGAGGACGGC CAGGTGCCCC CCGAGGACGA TGAGTATGAA 
TACTCTGAGT ATTCCATGGA GGAATACCAG GACCCCGCGG CTCCTTGGGA TGACGTTGAC 
CCCTGCTCGC TTCCACTGGA CGAGGGTTCC TGCAGTGCCT ACACCCTGCG CTGGTATCAT 
CGGGCTGGGG CGGGGGGCAC GAAGGCCTGT CACCCCTTCG TCTACGGTGG CTGCGGAGGG 
AATGCCAACC GTTTTGGGAC CCGGGAGGCC TGTGAGCACC GCTGCCTGCC CCAGGCGGCC 
CACAGTCAAG GGACAGGTGC TGCCCGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697097.2
CDS	187..8979
Translation	MRLRLLVAALCAGILAGAPRVWAQPRERVSCTRLYAADIVFLLDGSSSIGRSNFREVRGFLEGLVLPFSGAAGAQGVRFAAVQYSDDPRTEFDLDALGSGGDVIRAIRELSYKGGNTRTGAAILHVADRVFLPQLARPGVPKVCILITDGKSQDMVDTAAQRLKGQGVKLFAVGIKNADPEELKRIASQPTSDFFFFVNDFSILRTLLPLVSRRVCTTAGGVPVTLPPDDSTSGPRDLLLEPGSQSLRVQWTAASGPVTGYKVQYAPLTGLGQPLPSELREVSVPAGETSVQLQGLRPLTEYQVTVVALYANSIGEAVSGTARTTALEGPEVTIQNTTAHSLLVAWQSVPGATGYRVTWRVVRGGATQQQELGSGQGSVLLRDLQPGTDYEVTVSALLGRSVGPATSLTARTDTSVEQTLRPIILGPTSILLSWNLVPEARGYRLEWRRESGLEVPQKVVLPSDVTRYQLDGLQPGTEYRLTLYTLLEGREVATPATVVPTELPVGPVTALQATELPGQRVRVSWSPVPGATEYRITVRSAQGVERSLVLPGSQTAFDLDDVRAGLSYTVRVSARVGGREGAASILTVRREPEPPLAIPGLRVMASDATRVRVAWGPVPGASGFRISWRTDNGLESSQTVPSDSTATDIVGLRPGTSYQVAVSALRGREEGPPAVIVARTDPLGPVRSVHVTRVSSSSVTITWNRVPGATGYRVSWRSGRGPEKSQLVSGEATVAELDGLEPDTEYTVRVWARVAGVDGTPASAVVRTDPRPVGSVSKLQIHNASSDVLRVTWVGVPGATAYRLAWGRSEGGPTRQQILPGNTDSAEIRGLEGGVSYSVRVTALIGDREGAPVSIVVTTPPEAPPALETLRVEQRGEHSLRLHWQPVPGARGFRLRWRPEGGQEQSRVLGPEVSSYDLDGLEPATHYRIWLSVLGTAGEGPPSEVTAYTESPRVPSTQLRVVDTSVDSVTLAWTPVSGVSSYILSWRPLRGPGQELPGASQTLPGTSSSQRVTGLELGISYLFSLTPVRDGVRGPEASITQSPACPRGLMDVVFLVHATRDNAHRSEAVKRALEHLVSALGPLGPQAVQVGLLSYSHRPSPLLSLNSSHDLGVILQKIRNIPYTDPSGNNLGTAVVTAHRYLLAPDAPGRRRHVPGVMVLLVDEPLRGDIFNSIREAQAAGLRVMMLGLAGADPEQLRRLVPGMDPSQTFFAVDDGLSLERAVSSLAAALCQTALTTQPQPEPCSVHCPKGQKGEPGQMGLKGQAGPPGPPGLPGRIGAPGPQGPPGSASAKGERGFPGADGPPGSPGRPGTPGAPGPKGSPGWAGPRGEPGERGPRGPKGEPGEPGQVIGGEGPGLPGQKGDPGPPGPPGSRGPLGDPGPRGPPGFPGTAVKGEKGDRGEQGPPGPGDGTAALGEPGLPGLPGSPGPQGSVGPPGEKGEKGDCEDGAPGLPGQPGIPGERGLRGLPGDAGPKGDRGLTGAAGEKGERGSPGPVGPQGLPGVAGRPGPEGPEGPPGPTGRRGEKGEPGRPGDPAVGPGGAGAKGEKGDMGPPGPKGATGFKGEQGSPGLALPGDPGPKGDPGGRGPIGLTGRAGPPGDSGPPGEKGDPGRPGSPGPVGPRGRDGEVGEKGDDGPPGDPGLPGKAGERGLRGAPGARGPVGEKGDQGDPGEEGRNGSPGPSGPKGDRGEPGPPGAPGRLVDVGLGAGEKGEPGDRGQEGPRGPKGDPGPPGASGERGVSGLRGPPGPQGDPGVRGPAGEKGDRGPPGLDGRSGLDGKPGASGPPGPNGAMGKAGDPGRDGLPGLRGEQGPPGPAGPPGAPGKPGEDGKPGLNGKNGEPGDPGEDGRKGEKGDSGAPGREGRDGPKGERGAPGSPGLQGPPGLPGQVGPPGQGFPGVPGSSGPKGDRGETGPRGEQGLPGERGLRGEPGSLVNAERLLENIGIKTSALREIVETWEESSGGFLPTPERRRGPKGDPGERGPPGKEGPIGFSGERGPKGDRGDPGPQGPPGLALGERGLPGPTGLAGEPGKPGIPGLPGPAGSVGEAGRPGERGERGEKGERGEQGRDGLPGLPGPPGPPGPKVAVDEPGSGLSREQGPPGFKGAKGDPGSDGDRGPKGDRGEPGIKGDRGEPGQRGPNGSPGLPGERGVAGPEGKPGLQGPRGTPGPAGGHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLPGPTGAVGLPGPPGPSGLVGPQGAPGLPGQVGETGKPGAPGRDGATGKDGDRGAPGVPGSPGLPGPVGPKGEPGPMGTPGQAVVGPPGAKGEKGAPGALAGDLVGEPGAKGDRGLPGPRGEKGEAGRAGEPGDPGEDGQKGAPGPKGHKGDPGVGVQGPPGPVGPPGLKGDSGPPGSPGPPGIVGFPGQTGPRGELGQPGPSGERGLAGAPGREGAPGPLGPPGPPGSAGVPGVPGLKGDKGDTGAGLPGARGERGEPGVRGEDGRPGLEGPRGLAGPPGIRGERGEKGDAGASGLKGDKGDSAVILGPPGPRGAKGDSGERGPRGIDGDKGPRGDSGEPGEKGTKGEPGDKGSAGLLGARGLMGPKGEPGAAGIPGEPGSPGKDGVPGVRGDKGDVGFMGPRGLKGERGIKGACGLDGEKGDKGEAGPPGRPGLAGRRGELGEPGVPGQAGAPGKEGLIGPKGDRGFDGQPGPKGDQGEKGERGPPGVGGFPGPRGSDGSSGPPGPPGSIGPKGPEGLQGQKGERGPPGESVVGAPGAPGTPGERGEQGRPGPAGPRGEKGEAALTEDDIRGLVRQEMSQHCACQGQYIASGSRPLPSYAADTAGPQLHPVPVLRVSHAEEDGQVPPEDDEYEYSEYSMEEYQDPAAPWDDVDPCSLPLDEGSCSAYTLRWYHRAGAGGTKACHPFVYGGCGGNANRFGTREACEHRCLPQAAHSQGTGAARS

Target ORF information:

RefSeq Version	XM_002697051.6
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VII alpha 1 chain (COL7A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697051.6

ORF Insert Sequence:

ATGAGGCTGC GGCTCCTGGT GGCTGCGCTC TGCGCCGGGA TCCTGGCAGG GGCTCCCCGA 
GTGTGGGCCC AGCCCAGGGA GAGAGTGAGC TGCACGCGCC TCTACGCCGC TGACATCGTG 
TTCCTCCTTG ACGGCTCCTC GTCCATCGGC CGAAGCAACT TCCGCGAAGT GCGGGGTTTC 
CTTGAGGGGC TGGTGCTGCC CTTCTCCGGG GCAGCTGGGG CACAGGGTGT GCGCTTCGCG 
GCCGTGCAGT ACAGCGACGA CCCACGGACA GAATTTGACC TGGATGCACT TGGCTCAGGC 
GGAGATGTGA TCCGTGCCAT CCGAGAGCTC AGCTACAAGG GGGGCAACAC GCGCACAGGG 
GCCGCGATTC TCCACGTGGC TGACCGCGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GTATCCTCAT CACAGACGGG AAGTCCCAGG ACATGGTGGA CACAGCTGCC 
CAGAGGTTGA AGGGGCAGGG AGTCAAGCTG TTTGCTGTGG GCATCAAGAA TGCCGACCCC 
GAGGAGCTGA AGCGAATTGC CTCACAGCCG ACTAGCGATT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACGCT CCTGCCTCTG GTTTCCCGGA GGGTGTGCAC AACTGCGGGT 
GGTGTGCCCG TGACCCTGCC CCCCGACGAC TCGACCTCTG GTCCACGAGA CCTGCTGCTT 
GAGCCAGGCA GTCAGTCCTT GAGAGTCCAG TGGACAGCGG CCAGTGGCCC CGTGACTGGC 
TACAAGGTCC AGTACGCACC CCTGACAGGG CTGGGACAGC CATTGCCAAG CGAGCTGCGG 
GAGGTGAGCG TCCCAGCTGG TGAGACCAGC GTGCAGCTGC AGGGTCTCCG GCCACTGACT 
GAGTACCAAG TGACGGTGGT CGCCCTCTAC GCCAACAGCA TCGGGGAGGC CGTGAGCGGG 
ACCGCTAGGA CCACTGCTCT GGAGGGGCCA GAAGTGACCA TCCAGAACAC CACAGCCCAC 
AGCCTCCTGG TGGCCTGGCA GAGTGTGCCA GGTGCCACTG GCTACCGGGT GACGTGGCGG 
GTCGTCAGGG GTGGGGCCAC ACAGCAGCAG GAGCTGGGCT CTGGGCAGGG GTCAGTGTTG 
CTGAGGGACC TGCAGCCTGG CACCGACTAC GAGGTGACCG TGAGTGCCCT GCTTGGCCGG 
AGCGTGGGGC CAGCCACCTC CCTGACTGCC CGCACTGACA CGTCCGTCGA GCAGACCCTG 
CGTCCTATCA TCCTGGGCCC CACGTCCATC CTCCTCTCCT GGAACCTGGT GCCTGAGGCC 
CGTGGCTACC GGCTGGAATG GCGGCGTGAG AGCGGCTTAG AGGTGCCACA GAAGGTGGTG 
CTGCCCTCTG ACGTGACCCG CTACCAGTTG GATGGGCTGC AGCCAGGCAC CGAGTACCGC 
CTCACTCTCT ACACGCTGCT AGAGGGCCGT GAGGTGGCCA CCCCAGCGAC CGTGGTCCCC 
ACTGAGCTGC CCGTGGGCCC CGTCACAGCC CTCCAGGCCA CCGAGCTGCC TGGGCAGCGT 
GTGCGAGTGT CCTGGAGCCC AGTTCCCGGC GCCACCGAGT ATCGCATCAC TGTGCGCAGC 
GCCCAGGGGG TTGAGCGGAG CCTGGTGCTT CCTGGGAGTC AGACGGCCTT TGACTTGGAT 
GACGTCCGGG CTGGGCTCAG CTACACAGTG CGGGTGTCAG CGCGAGTAGG CGGCCGGGAG 
GGCGCTGCCA GCATCCTCAC TGTCCGCCGG GAGCCAGAAC CCCCACTGGC CATCCCAGGG 
CTGCGGGTCA TGGCATCGGA CGCAACGCGA GTGAGGGTGG CCTGGGGACC AGTTCCCGGA 
GCCAGCGGAT TTCGGATTAG CTGGAGGACA GACAATGGTC TGGAGTCCAG CCAGACAGTT 
CCTTCAGACT CTACTGCCAC CGACATTGTG GGGCTGAGAC CTGGGACCTC CTACCAGGTG 
GCCGTGTCGG CGCTGCGAGG AAGAGAGGAG GGCCCTCCTG CGGTCATCGT GGCTCGAACC 
GATCCCTTGG GCCCAGTGAG ATCGGTCCAT GTGACTCGAG TCAGCAGCTC ATCTGTCACC 
ATCACCTGGA ACCGGGTTCC TGGTGCCACA GGGTACAGGG TCTCCTGGCG CTCAGGCCGA 
GGCCCAGAGA AATCCCAGTT GGTTTCTGGG GAGGCCACGG TGGCCGAGCT GGATGGACTG 
GAGCCAGATA CTGAGTACAC CGTGCGTGTG TGGGCCCGTG TGGCTGGTGT GGACGGGACG 
CCTGCCTCTG CGGTTGTGAG AACTGACCCT CGGCCCGTGG GCAGTGTGTC GAAGCTGCAG 
ATCCATAATG CTTCCAGTGA CGTTCTGCGG GTCACCTGGG TGGGGGTCCC TGGAGCCACA 
GCCTACAGAC TGGCCTGGGG CCGGAGTGAG GGTGGCCCCA CAAGACAGCA GATTCTCCCA 
GGAAACACGG ACTCCGCAGA AATACGGGGC CTCGAAGGCG GAGTCAGTTA TTCAGTGAGA 
GTGACCGCAC TCATAGGGGA CCGCGAGGGC GCGCCTGTCT CCATTGTCGT CACCACCCCT 
CCGGAGGCTC CTCCAGCCCT GGAGACACTT CGCGTGGAGC AGCGAGGGGA GCACTCACTG 
AGGCTGCACT GGCAGCCGGT GCCTGGTGCA CGCGGCTTCC GTCTGCGTTG GCGACCTGAG 
GGTGGCCAGG AACAGTCCCG AGTCCTGGGG CCGGAAGTCA GCAGCTATGA CCTGGATGGG 
CTGGAGCCAG CGACCCACTA CCGCATATGG CTGAGCGTCT TGGGGACGGC TGGAGAAGGG 
CCCCCCTCGG AAGTGACTGC ATACACTGAG TCACCTCGTG TCCCAAGCAC TCAACTGCGT 
GTGGTGGACA CGTCAGTCGA CTCAGTGACT CTGGCCTGGA CCCCAGTGTC TGGGGTATCC 
AGCTACATCC TATCCTGGCG GCCTCTCAGA GGCCCTGGCC AAGAACTGCC TGGGGCCTCA 
CAGACACTTC CGGGGACCTC AAGTTCCCAG CGGGTGACAG GCCTAGAGCT GGGCATCTCC 
TACCTCTTCT CCCTAACTCC TGTCCGGGAC GGTGTACGCG GTCCTGAAGC CTCTATCACA 
CAGAGCCCAG CGTGTCCCCG CGGCCTGATG GATGTGGTGT TCCTGGTGCA CGCCACTCGA 
GACAATGCTC ATCGCTCGGA GGCTGTGAAG CGAGCCCTGG AGCACCTGGT GTCTGCACTC 
GGGCCTCTCG GGCCACAGGC CGTCCAGGTC GGCCTCCTGT CCTACAGTCA CCGGCCCTCC 
CCACTGTTGT CGCTGAACAG CTCCCATGAC CTTGGCGTCA TCCTGCAGAA GATCCGCAAC 
ATCCCCTACA CGGACCCAAG TGGGAACAAC CTGGGCACAG CCGTGGTTAC AGCCCACAGA 
TACCTGCTGG CACCAGATGC GCCTGGACGC CGCCGGCATG TGCCGGGGGT GATGGTTCTC 
CTGGTGGATG AGCCCTTGAG AGGTGACATC TTCAACTCTA TCCGTGAGGC CCAGGCTGCT 
GGGCTCAGAG TGATGATGCT GGGGCTGGCG GGAGCTGACC CAGAGCAGCT GCGTCGCTTG 
GTGCCTGGCA TGGACCCTTC CCAGACCTTC TTCGCTGTGG ATGATGGTCT GAGCTTGGAG 
CGGGCCGTCA GTAGTCTGGC AGCAGCCCTG TGTCAGACAG CCTTGACCAC GCAGCCACAG 
CCAGAGCCTT GCTCAGTGCA TTGTCCAAAG GGCCAGAAGG GGGAACCCGG ACAGATGGGC 
CTGAAAGGAC AAGCCGGGCC TCCTGGCCCC CCCGGCCTCC CGGGCAGGAT TGGTGCTCCT 
GGCCCCCAGG GACCTCCTGG AAGTGCTTCT GCGAAGGGCG AGAGGGGCTT CCCTGGGGCA 
GATGGGCCTC CAGGCAGCCC TGGCCGCCCT GGGACTCCTG GAGCCCCTGG TCCGAAGGGC 
TCCCCAGGGT GGGCCGGCCC TCGTGGAGAA CCAGGAGAGC GAGGACCTCG AGGCCCAAAG 
GGGGAGCCGG GAGAGCCTGG ACAAGTCATT GGAGGCGAGG GCCCAGGGCT TCCTGGGCAG 
AAAGGGGACC CTGGACCTCC AGGTCCCCCT GGATCTCGTG GGCCGTTGGG GGACCCGGGA 
CCCCGGGGTC CCCCGGGATT TCCTGGAACA GCTGTGAAGG GTGAGAAAGG CGATCGTGGG 
GAGCAGGGCC CCCCTGGACC AGGTGACGGC ACTGCTGCTC TGGGCGAGCC TGGGCTGCCG 
GGTCTTCCTG GAAGCCCCGG ACCCCAAGGC TCAGTTGGCC CCCCTGGAGA GAAAGGAGAA 
AAGGGTGACT GTGAAGATGG AGCCCCGGGC CTCCCAGGAC AACCCGGAAT TCCGGGTGAG 
CGGGGCCTTC GGGGACTTCC TGGAGATGCT GGCCCCAAAG GCGACCGAGG ACTAACAGGG 
GCCGCTGGAG AGAAGGGTGA ACGTGGATCG CCTGGCCCAG TGGGACCACA GGGGCTGCCA 
GGAGTTGCTG GACGACCTGG GCCCGAGGGT CCTGAAGGGC CACCAGGACC GACCGGCCGC 
CGAGGAGAGA AGGGGGAGCC TGGTCGGCCT GGAGACCCTG CAGTGGGACC TGGGGGTGCT 
GGAGCTAAAG GAGAGAAGGG AGATATGGGC CCCCCCGGGC CCAAAGGAGC TACCGGATTC 
AAAGGGGAAC AGGGCTCACC TGGCTTGGCT CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC 
CCTGGAGGCC GAGGTCCCAT TGGCCTCACT GGCAGAGCAG GACCCCCGGG CGACTCAGGA 
CCCCCTGGAG AGAAGGGAGA CCCTGGGCGG CCCGGTTCCC CAGGACCCGT CGGCCCCCGA 
GGACGAGACG GTGAAGTTGG AGAGAAGGGC GATGACGGTC CCCCGGGTGA CCCAGGTTTG 
CCTGGAAAAG CTGGCGAGCG TGGCCTTCGG GGAGCGCCTG GAGCTCGGGG GCCTGTGGGT 
GAGAAGGGAG ACCAGGGAGA TCCTGGAGAG GAAGGACGAA ATGGGAGCCC TGGACCGTCT 
GGACCCAAGG GTGACCGTGG GGAGCCAGGT CCCCCAGGAG CCCCTGGACG GCTGGTGGAC 
GTGGGACTTG GAGCCGGAGA GAAGGGAGAG CCTGGGGACC GAGGACAGGA GGGTCCACGA 
GGACCCAAGG GCGACCCTGG CCCCCCTGGA GCTTCTGGGG AGAGGGGCGT CAGTGGACTT 
CGGGGGCCTC CAGGCCCACA GGGGGACCCA GGTGTTCGAG GCCCAGCAGG AGAAAAGGGC 
GACCGGGGCC CTCCTGGCCT GGATGGCCGC AGTGGGCTGG ATGGGAAACC GGGAGCCTCT 
GGTCCCCCTG GGCCGAATGG TGCCATGGGC AAGGCCGGAG ACCCAGGGAG AGACGGACTT 
CCGGGCCTTC GAGGAGAACA GGGCCCCCCT GGCCCTGCTG GTCCCCCTGG AGCACCGGGA 
AAGCCGGGCG AGGATGGCAA GCCTGGCCTG AATGGGAAAA ACGGAGAACC CGGGGACCCT 
GGAGAAGACG GGAGGAAGGG AGAGAAGGGA GATTCAGGCG CCCCTGGGAG AGAAGGTCGT 
GACGGCCCCA AGGGTGAGCG TGGAGCTCCT GGTAGCCCTG GACTCCAGGG CCCCCCGGGC 
CTCCCAGGGC AGGTCGGCCC TCCCGGGCAG GGCTTCCCTG GCGTCCCGGG GAGTTCAGGC 
CCCAAGGGTG ACCGTGGGGA GACTGGACCC AGAGGGGAGC AGGGCCTCCC AGGAGAGCGT 
GGCCTGAGAG GAGAGCCTGG GAGCCTGGTG AATGCAGAGC GGTTGCTGGA AAATATTGGC 
ATCAAGACAT CTGCCCTGAG GGAGATCGTG GAGACCTGGG AAGAAAGCTC TGGTGGCTTC 
CTGCCCACGC CTGAACGGCG TCGCGGCCCC AAGGGGGACC CAGGCGAGCG GGGCCCCCCA 
GGCAAGGAGG GCCCCATCGG CTTTTCTGGA GAACGTGGAC CAAAGGGAGA TCGTGGAGAC 
CCCGGCCCTC AGGGGCCACC TGGCCTGGCC CTGGGGGAGA GGGGCCTTCC TGGACCTACC 
GGCCTTGCCG GGGAGCCTGG GAAGCCTGGT ATCCCTGGGC TCCCTGGCCC AGCTGGGAGT 
GTGGGGGAGG CCGGAAGACC CGGAGAGAGG GGAGAACGGG GAGAGAAAGG AGAACGTGGA 
GAACAGGGCA GAGACGGCCT TCCTGGCCTC CCTGGACCCC CCGGACCCCC AGGCCCCAAG 
GTGGCCGTGG ACGAGCCAGG TTCTGGGCTC TCTAGAGAAC AAGGACCCCC TGGATTCAAG 
GGTGCTAAGG GGGATCCAGG CAGTGATGGC GACCGAGGCC CCAAAGGAGA CAGGGGTGAG 
CCGGGCATCA AGGGCGACCG GGGAGAGCCT GGACAGAGGG GTCCGAATGG CAGCCCGGGT 
CTGCCAGGAG AGCGCGGCGT GGCTGGGCCC GAGGGGAAGC CGGGTCTGCA AGGTCCACGG 
GGGACCCCTG GCCCGGCGGG CGGCCATGGA GACCCTGGAC CACCCGGGGC CCCGGGTCTT 
GCTGGCCCTG CGGGACCCCA GGGACCTTCT GGCCTAAAGG GGGAGCCCGG AGAGACAGGA 
CCACCAGGAC GGGGCCTGCC TGGACCTACT GGAGCCGTGG GACTTCCCGG GCCCCCTGGC 
CCTTCAGGCC TGGTGGGTCC TCAAGGGGCA CCGGGTTTGC CTGGACAAGT GGGGGAGACG 
GGAAAGCCGG GAGCGCCAGG TCGTGATGGT GCCACTGGGA AAGATGGAGA CAGAGGAGCC 
CCCGGTGTGC CGGGGTCACC AGGTCTGCCA GGCCCTGTCG GACCTAAAGG AGAGCCGGGA 
CCCATGGGGA CCCCTGGACA GGCTGTGGTC GGGCCCCCTG GAGCAAAGGG AGAGAAGGGT 
GCCCCTGGAG CCCTTGCTGG AGACCTGGTG GGAGAGCCGG GAGCCAAGGG TGACCGAGGA 
CTGCCAGGGC CGCGGGGCGA GAAGGGTGAA GCTGGCCGTG CGGGGGAGCC TGGAGACCCT 
GGCGAAGATG GTCAGAAGGG GGCTCCGGGA CCCAAAGGTC ACAAGGGTGA CCCAGGAGTT 
GGGGTCCAGG GGCCCCCTGG GCCAGTTGGC CCTCCAGGTC TAAAGGGAGA CTCAGGGCCC 
CCCGGATCCC CCGGACCTCC TGGTATTGTG GGGTTCCCCG GTCAGACAGG CCCTCGAGGA 
GAGCTGGGGC AGCCGGGCCC CAGTGGAGAG CGGGGTCTGG CAGGTGCCCC AGGGAGAGAG 
GGAGCCCCAG GTCCCTTGGG GCCGCCTGGA CCACCTGGGT CAGCGGGAGT ACCTGGGGTC 
CCTGGACTCA AAGGAGACAA GGGTGACACT GGAGCAGGGC TGCCTGGGGC CCGAGGCGAG 
CGTGGGGAGC CCGGTGTCCG GGGTGAAGAT GGCCGCCCCG GCCTGGAGGG ACCCCGAGGA 
CTTGCGGGTC CCCCAGGCAT CCGGGGAGAA CGTGGGGAGA AGGGTGACGC TGGTGCCTCA 
GGACTGAAGG GTGACAAGGG CGACTCAGCT GTGATTTTGG GGCCTCCGGG GCCACGGGGT 
GCCAAGGGGG ACTCGGGTGA ACGAGGGCCT CGGGGAATAG ATGGTGACAA AGGACCTCGG 
GGAGACAGCG GGGAACCTGG AGAGAAGGGC ACCAAGGGAG AGCCTGGTGA CAAGGGCTCA 
GCCGGGCTGC TGGGAGCGCG CGGACTCATG GGACCCAAGG GCGAGCCTGG TGCCGCAGGG 
ATCCCCGGCG AACCGGGATC CCCAGGAAAA GACGGAGTCC CTGGTGTCCG AGGAGACAAA 
GGAGATGTTG GCTTCATGGG TCCCCGGGGC CTCAAGGGTG AACGGGGAAT TAAGGGAGCC 
TGTGGCCTCG ACGGAGAGAA GGGAGATAAG GGAGAAGCCG GCCCCCCGGG CCGCCCAGGG 
CTGGCAGGAC GCAGAGGAGA GCTGGGGGAG CCGGGTGTTC CAGGCCAGGC AGGGGCCCCT 
GGGAAGGAGG GCCTGATCGG TCCCAAGGGT GACCGAGGTT TCGATGGGCA GCCAGGACCC 
AAGGGTGACC AGGGCGAGAA AGGGGAGCGG GGACCCCCAG GAGTGGGGGG CTTCCCAGGT 
CCCAGGGGCA GTGATGGCTC TAGTGGTCCT CCCGGGCCAC CTGGCAGCAT TGGTCCCAAA 
GGCCCTGAAG GACTTCAGGG TCAGAAGGGT GAGCGAGGTC CCCCTGGAGA GAGCGTGGTG 
GGTGCCCCTG GGGCCCCTGG AACTCCTGGA GAGAGAGGGG AGCAGGGGCG ACCAGGCCCC 
GCGGGGCCCC GTGGTGAAAA GGGAGAAGCT GCGCTGACGG AGGATGACAT TCGGGGCCTT 
GTGCGTCAGG AGATGAGTCA ACATTGCGCC TGTCAGGGCC AGTATATCGC ATCTGGATCA 
CGGCCTCTCC CGAGTTACGC AGCAGACACC GCTGGCCCGC AGCTCCACCC CGTGCCCGTG 
CTCCGCGTGT CCCACGCAGA GGAGGACGGC CAGGTGCCCC CCGAGGACGA TGAGTATGAA 
TACTCTGAGT ATTCCATGGA GGAATACCAG GACCCCGCGG CTCCTTGGGA TGACGTTGAC 
CCCTGCTCGC TTCCACTGGA CGAGGGTTCC TGCAGTGCCT ACACCCTGCG CTGGTATCAT 
CGGGCTGGGG CGGGGGGCAC GAAGGCCTGT CACCCCTTCG TCTACGGTGG CTGCGGAGGG 
AATGCCAACC GTTTTGGGAC CCGGGAGGCC TGTGAGCACC GCTGCCTGCC CCAGGCGGCC 
CACAGTCAAG GGACAGGTGC TGCCCGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL7A1 ( XM_002697051.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697097.2 Bos taurus collagen type VII alpha 1 (COL7A1), mRNA.

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CloneID	OBa31702
Clone ID Related Accession (Same CDS sequence)	XM_002697051.5 , XM_015468790.1
Accession Version	XM_002697051.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-1(VII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697051.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTGC GGCTCCTGGT GGCTGCGCTC TGCGCCGGGA TCCTGGCAGG GGCTCCCCGA 
GTGTGGGCCC AGCCCAGGGA GAGAGTGAGC TGCACGCGCC TCTACGCCGC TGACATCGTG 
TTCCTCCTTG ACGGCTCCTC GTCCATCGGC CGAAGCAACT TCCGCGAAGT GCGGGGTTTC 
CTTGAGGGGC TGGTGCTGCC CTTCTCCGGG GCAGCTGGGG CACAGGGTGT GCGCTTCGCG 
GCCGTGCAGT ACAGCGACGA CCCACGGACA GAATTTGACC TGGATGCACT TGGCTCAGGC 
GGAGATGTGA TCCGTGCCAT CCGAGAGCTC AGCTACAAGG GGGGCAACAC GCGCACAGGG 
GCCGCGATTC TCCACGTGGC TGACCGCGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GTATCCTCAT CACAGACGGG AAGTCCCAGG ACATGGTGGA CACAGCTGCC 
CAGAGGTTGA AGGGGCAGGG AGTCAAGCTG TTTGCTGTGG GCATCAAGAA TGCCGACCCC 
GAGGAGCTGA AGCGAATTGC CTCACAGCCG ACTAGCGATT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACGCT CCTGCCTCTG GTTTCCCGGA GGGTGTGCAC AACTGCGGGT 
GGTGTGCCCG TGACCCTGCC CCCCGACGAC TCGACCTCTG GTCCACGAGA CCTGCTGCTT 
GAGCCAGGCA GTCAGTCCTT GAGAGTCCAG TGGACAGCGG CCAGTGGCCC CGTGACTGGC 
TACAAGGTCC AGTACGCACC CCTGACAGGG CTGGGACAGC CATTGCCAAG CGAGCTGCGG 
GAGGTGAGCG TCCCAGCTGG TGAGACCAGC GTGCAGCTGC AGGGTCTCCG GCCACTGACT 
GAGTACCAAG TGACGGTGGT CGCCCTCTAC GCCAACAGCA TCGGGGAGGC CGTGAGCGGG 
ACCGCTAGGA CCACTGCTCT GGAGGGGCCA GAAGTGACCA TCCAGAACAC CACAGCCCAC 
AGCCTCCTGG TGGCCTGGCA GAGTGTGCCA GGTGCCACTG GCTACCGGGT GACGTGGCGG 
GTCGTCAGGG GTGGGGCCAC ACAGCAGCAG GAGCTGGGCT CTGGGCAGGG GTCAGTGTTG 
CTGAGGGACC TGCAGCCTGG CACCGACTAC GAGGTGACCG TGAGTGCCCT GCTTGGCCGG 
AGCGTGGGGC CAGCCACCTC CCTGACTGCC CGCACTGACA CGTCCGTCGA GCAGACCCTG 
CGTCCTATCA TCCTGGGCCC CACGTCCATC CTCCTCTCCT GGAACCTGGT GCCTGAGGCC 
CGTGGCTACC GGCTGGAATG GCGGCGTGAG AGCGGCTTAG AGGTGCCACA GAAGGTGGTG 
CTGCCCTCTG ACGTGACCCG CTACCAGTTG GATGGGCTGC AGCCAGGCAC CGAGTACCGC 
CTCACTCTCT ACACGCTGCT AGAGGGCCGT GAGGTGGCCA CCCCAGCGAC CGTGGTCCCC 
ACTGAGCTGC CCGTGGGCCC CGTCACAGCC CTCCAGGCCA CCGAGCTGCC TGGGCAGCGT 
GTGCGAGTGT CCTGGAGCCC AGTTCCCGGC GCCACCGAGT ATCGCATCAC TGTGCGCAGC 
GCCCAGGGGG TTGAGCGGAG CCTGGTGCTT CCTGGGAGTC AGACGGCCTT TGACTTGGAT 
GACGTCCGGG CTGGGCTCAG CTACACAGTG CGGGTGTCAG CGCGAGTAGG CGGCCGGGAG 
GGCGCTGCCA GCATCCTCAC TGTCCGCCGG GAGCCAGAAC CCCCACTGGC CATCCCAGGG 
CTGCGGGTCA TGGCATCGGA CGCAACGCGA GTGAGGGTGG CCTGGGGACC AGTTCCCGGA 
GCCAGCGGAT TTCGGATTAG CTGGAGGACA GACAATGGTC TGGAGTCCAG CCAGACAGTT 
CCTTCAGACT CTACTGCCAC CGACATTGTG GGGCTGAGAC CTGGGACCTC CTACCAGGTG 
GCCGTGTCGG CGCTGCGAGG AAGAGAGGAG GGCCCTCCTG CGGTCATCGT GGCTCGAACC 
GATCCCTTGG GCCCAGTGAG ATCGGTCCAT GTGACTCGAG TCAGCAGCTC ATCTGTCACC 
ATCACCTGGA ACCGGGTTCC TGGTGCCACA GGGTACAGGG TCTCCTGGCG CTCAGGCCGA 
GGCCCAGAGA AATCCCAGTT GGTTTCTGGG GAGGCCACGG TGGCCGAGCT GGATGGACTG 
GAGCCAGATA CTGAGTACAC CGTGCGTGTG TGGGCCCGTG TGGCTGGTGT GGACGGGACG 
CCTGCCTCTG CGGTTGTGAG AACTGACCCT CGGCCCGTGG GCAGTGTGTC GAAGCTGCAG 
ATCCATAATG CTTCCAGTGA CGTTCTGCGG GTCACCTGGG TGGGGGTCCC TGGAGCCACA 
GCCTACAGAC TGGCCTGGGG CCGGAGTGAG GGTGGCCCCA CAAGACAGCA GATTCTCCCA 
GGAAACACGG ACTCCGCAGA AATACGGGGC CTCGAAGGCG GAGTCAGTTA TTCAGTGAGA 
GTGACCGCAC TCATAGGGGA CCGCGAGGGC GCGCCTGTCT CCATTGTCGT CACCACCCCT 
CCGGAGGCTC CTCCAGCCCT GGAGACACTT CGCGTGGAGC AGCGAGGGGA GCACTCACTG 
AGGCTGCACT GGCAGCCGGT GCCTGGTGCA CGCGGCTTCC GTCTGCGTTG GCGACCTGAG 
GGTGGCCAGG AACAGTCCCG AGTCCTGGGG CCGGAAGTCA GCAGCTATGA CCTGGATGGG 
CTGGAGCCAG CGACCCACTA CCGCATATGG CTGAGCGTCT TGGGGACGGC TGGAGAAGGG 
CCCCCCTCGG AAGTGACTGC ATACACTGAG TCACCTCGTG TCCCAAGCAC TCAACTGCGT 
GTGGTGGACA CGTCAGTCGA CTCAGTGACT CTGGCCTGGA CCCCAGTGTC TGGGGTATCC 
AGCTACATCC TATCCTGGCG GCCTCTCAGA GGCCCTGGCC AAGAACTGCC TGGGGCCTCA 
CAGACACTTC CGGGGACCTC AAGTTCCCAG CGGGTGACAG GCCTAGAGCT GGGCATCTCC 
TACCTCTTCT CCCTAACTCC TGTCCGGGAC GGTGTACGCG GTCCTGAAGC CTCTATCACA 
CAGAGCCCAG CGTGTCCCCG CGGCCTGATG GATGTGGTGT TCCTGGTGCA CGCCACTCGA 
GACAATGCTC ATCGCTCGGA GGCTGTGAAG CGAGCCCTGG AGCACCTGGT GTCTGCACTC 
GGGCCTCTCG GGCCACAGGC CGTCCAGGTC GGCCTCCTGT CCTACAGTCA CCGGCCCTCC 
CCACTGTTGT CGCTGAACAG CTCCCATGAC CTTGGCGTCA TCCTGCAGAA GATCCGCAAC 
ATCCCCTACA CGGACCCAAG TGGGAACAAC CTGGGCACAG CCGTGGTTAC AGCCCACAGA 
TACCTGCTGG CACCAGATGC GCCTGGACGC CGCCGGCATG TGCCGGGGGT GATGGTTCTC 
CTGGTGGATG AGCCCTTGAG AGGTGACATC TTCAACTCTA TCCGTGAGGC CCAGGCTGCT 
GGGCTCAGAG TGATGATGCT GGGGCTGGCG GGAGCTGACC CAGAGCAGCT GCGTCGCTTG 
GTGCCTGGCA TGGACCCTTC CCAGACCTTC TTCGCTGTGG ATGATGGTCT GAGCTTGGAG 
CGGGCCGTCA GTAGTCTGGC AGCAGCCCTG TGTCAGACAG CCTTGACCAC GCAGCCACAG 
CCAGAGCCTT GCTCAGTGCA TTGTCCAAAG GGCCAGAAGG GGGAACCCGG ACAGATGGGC 
CTGAAAGGAC AAGCCGGGCC TCCTGGCCCC CCCGGCCTCC CGGGCAGGAT TGGTGCTCCT 
GGCCCCCAGG GACCTCCTGG AAGTGCTTCT GCGAAGGGCG AGAGGGGCTT CCCTGGGGCA 
GATGGGCCTC CAGGCAGCCC TGGCCGCCCT GGGACTCCTG GAGCCCCTGG TCCGAAGGGC 
TCCCCAGGGT GGGCCGGCCC TCGTGGAGAA CCAGGAGAGC GAGGACCTCG AGGCCCAAAG 
GGGGAGCCGG GAGAGCCTGG ACAAGTCATT GGAGGCGAGG GCCCAGGGCT TCCTGGGCAG 
AAAGGGGACC CTGGACCTCC AGGTCCCCCT GGATCTCGTG GGCCGTTGGG GGACCCGGGA 
CCCCGGGGTC CCCCGGGATT TCCTGGAACA GCTGTGAAGG GTGAGAAAGG CGATCGTGGG 
GAGCAGGGCC CCCCTGGACC AGGTGACGGC ACTGCTGCTC TGGGCGAGCC TGGGCTGCCG 
GGTCTTCCTG GAAGCCCCGG ACCCCAAGGC TCAGTTGGCC CCCCTGGAGA GAAAGGAGAA 
AAGGGTGACT GTGAAGATGG AGCCCCGGGC CTCCCAGGAC AACCCGGAAT TCCGGGTGAG 
CGGGGCCTTC GGGGACTTCC TGGAGATGCT GGCCCCAAAG GCGACCGAGG ACTAACAGGG 
GCCGCTGGAG AGAAGGGTGA ACGTGGATCG CCTGGCCCAG TGGGACCACA GGGGCTGCCA 
GGAGTTGCTG GACGACCTGG GCCCGAGGGT CCTGAAGGGC CACCAGGACC GACCGGCCGC 
CGAGGAGAGA AGGGGGAGCC TGGTCGGCCT GGAGACCCTG CAGTGGGACC TGGGGGTGCT 
GGAGCTAAAG GAGAGAAGGG AGATATGGGC CCCCCCGGGC CCAAAGGAGC TACCGGATTC 
AAAGGGGAAC AGGGCTCACC TGGCTTGGCT CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC 
CCTGGAGGCC GAGGTCCCAT TGGCCTCACT GGCAGAGCAG GACCCCCGGG CGACTCAGGA 
CCCCCTGGAG AGAAGGGAGA CCCTGGGCGG CCCGGTTCCC CAGGACCCGT CGGCCCCCGA 
GGACGAGACG GTGAAGTTGG AGAGAAGGGC GATGACGGTC CCCCGGGTGA CCCAGGTTTG 
CCTGGAAAAG CTGGCGAGCG TGGCCTTCGG GGAGCGCCTG GAGCTCGGGG GCCTGTGGGT 
GAGAAGGGAG ACCAGGGAGA TCCTGGAGAG GAAGGACGAA ATGGGAGCCC TGGACCGTCT 
GGACCCAAGG GTGACCGTGG GGAGCCAGGT CCCCCAGGAG CCCCTGGACG GCTGGTGGAC 
GTGGGACTTG GAGCCGGAGA GAAGGGAGAG CCTGGGGACC GAGGACAGGA GGGTCCACGA 
GGACCCAAGG GCGACCCTGG CCCCCCTGGA GCTTCTGGGG AGAGGGGCGT CAGTGGACTT 
CGGGGGCCTC CAGGCCCACA GGGGGACCCA GGTGTTCGAG GCCCAGCAGG AGAAAAGGGC 
GACCGGGGCC CTCCTGGCCT GGATGGCCGC AGTGGGCTGG ATGGGAAACC GGGAGCCTCT 
GGTCCCCCTG GGCCGAATGG TGCCATGGGC AAGGCCGGAG ACCCAGGGAG AGACGGACTT 
CCGGGCCTTC GAGGAGAACA GGGCCCCCCT GGCCCTGCTG GTCCCCCTGG AGCACCGGGA 
AAGCCGGGCG AGGATGGCAA GCCTGGCCTG AATGGGAAAA ACGGAGAACC CGGGGACCCT 
GGAGAAGACG GGAGGAAGGG AGAGAAGGGA GATTCAGGCG CCCCTGGGAG AGAAGGTCGT 
GACGGCCCCA AGGGTGAGCG TGGAGCTCCT GGTAGCCCTG GACTCCAGGG CCCCCCGGGC 
CTCCCAGGGC AGGTCGGCCC TCCCGGGCAG GGCTTCCCTG GCGTCCCGGG GAGTTCAGGC 
CCCAAGGGTG ACCGTGGGGA GACTGGACCC AGAGGGGAGC AGGGCCTCCC AGGAGAGCGT 
GGCCTGAGAG GAGAGCCTGG GAGCCTGGTG AATGCAGAGC GGTTGCTGGA AAATATTGGC 
ATCAAGACAT CTGCCCTGAG GGAGATCGTG GAGACCTGGG AAGAAAGCTC TGGTGGCTTC 
CTGCCCACGC CTGAACGGCG TCGCGGCCCC AAGGGGGACC CAGGCGAGCG GGGCCCCCCA 
GGCAAGGAGG GCCCCATCGG CTTTTCTGGA GAACGTGGAC CAAAGGGAGA TCGTGGAGAC 
CCCGGCCCTC AGGGGCCACC TGGCCTGGCC CTGGGGGAGA GGGGCCTTCC TGGACCTACC 
GGCCTTGCCG GGGAGCCTGG GAAGCCTGGT ATCCCTGGGC TCCCTGGCCC AGCTGGGAGT 
GTGGGGGAGG CCGGAAGACC CGGAGAGAGG GGAGAACGGG GAGAGAAAGG AGAACGTGGA 
GAACAGGGCA GAGACGGCCT TCCTGGCCTC CCTGGACCCC CCGGACCCCC AGGCCCCAAG 
GTGGCCGTGG ACGAGCCAGG TTCTGGGCTC TCTAGAGAAC AAGGACCCCC TGGATTCAAG 
GGTGCTAAGG GGGATCCAGG CAGTGATGGC GACCGAGGCC CCAAAGGAGA CAGGGGTGAG 
CCGGGCATCA AGGGCGACCG GGGAGAGCCT GGACAGAGGG GTCCGAATGG CAGCCCGGGT 
CTGCCAGGAG AGCGCGGCGT GGCTGGGCCC GAGGGGAAGC CGGGTCTGCA AGGTCCACGG 
GGGACCCCTG GCCCGGCGGG CGGCCATGGA GACCCTGGAC CACCCGGGGC CCCGGGTCTT 
GCTGGCCCTG CGGGACCCCA GGGACCTTCT GGCCTAAAGG GGGAGCCCGG AGAGACAGGA 
CCACCAGGAC GGGGCCTGCC TGGACCTACT GGAGCCGTGG GACTTCCCGG GCCCCCTGGC 
CCTTCAGGCC TGGTGGGTCC TCAAGGGGCA CCGGGTTTGC CTGGACAAGT GGGGGAGACG 
GGAAAGCCGG GAGCGCCAGG TCGTGATGGT GCCACTGGGA AAGATGGAGA CAGAGGAGCC 
CCCGGTGTGC CGGGGTCACC AGGTCTGCCA GGCCCTGTCG GACCTAAAGG AGAGCCGGGA 
CCCATGGGGA CCCCTGGACA GGCTGTGGTC GGGCCCCCTG GAGCAAAGGG AGAGAAGGGC 
GCCCCTGGAG CCCTTGCTGG AGACCTGGTG GGAGAGCCGG GAGCCAAGGG TGACCGAGGA 
CTGCCAGGGC CGCGGGGCGA GAAGGGTGAA GCTGGCCGTG CGGGGGAGCC TGGAGACCCT 
GGCGAAGATG GTCAGAAGGG GGCTCCGGGA CCCAAAGGTC ACAAGGGTGA CCCAGGAGTT 
GGGGTCCAGG GGCCCCCTGG GCCAGTTGGC CCTCCAGGTC TAAAGGGAGA CTCAGGGCCC 
CCCGGATCCC CCGGACCTCC TGGTATTGTG GGGTTCCCCG GTCAGACAGG CCCTCGAGGA 
GAGCTGGGGC AGCCGGGCCC CAGTGGAGAG CGGGGTCTGG CAGGTGCCCC AGGGAGAGAG 
GGAGCCCCAG GTCCCTTGGG GCCGCCTGGA CCACCTGGGT CAGCGGGAGT ACCTGGGGTC 
CCTGGACTCA AAGGAGACAA GGGTGACACT GGAGCAGGGC TGCCTGGGGC CCGAGGCGAG 
CGTGGGGAGC CCGGTGTCCG GGGTGAAGAT GGCCGCCCCG GCCTGGAGGG ACCCCGAGGA 
CTTGCGGGTC CCCCAGGCAT CCGGGGAGAA CGTGGGGAGA AGGGTGACGC TGGTGCCTCA 
GGACTGAAGG GTGACAAGGG CGACTCAGCT GTGATTTTGG GGCCTCCGGG GCCACGGGGT 
GCCAAGGGGG ACTCGGGTGA ACGAGGGCCT CGGGGAATAG ATGGTGACAA AGGACCTCGG 
GGAGACAGCG GGGAACCTGG AGAGAAGGGC ACCAAGGGAG AGCCTGGTGA CAAGGGCTCA 
GCCGGGCTGC TGGGAGCGCG CGGACTCATG GGACCCAAGG GCGAGCCTGG TGCCGCAGGG 
ATCCCCGGCG AACCGGGATC CCCAGGAAAA GACGGAGTCC CTGGTGTCCG AGGAGACAAA 
GGAGATGTTG GCTTCATGGG TCCCCGGGGC CTCAAGGGTG AACGGGGAAT TAAGGGAGCC 
TGTGGCCTCG ACGGAGAGAA GGGAGATAAG GGAGAAGCCG GCCCCCCGGG CCGCCCAGGG 
CTGGCAGGAC GCAGAGGAGA GCTGGGGGAG CCGGGTGTTC CAGGCCAGGC AGGGGCCCCT 
GGGAAGGAGG GCCTGATCGG TCCCAAGGGT GACCGAGGTT TCGATGGGCA GCCAGGACCC 
AAGGGTGACC AGGGCGAGAA AGGGGAGCGG GGACCCCCAG GAGTGGGGGG CTTCCCAGGT 
CCCAGGGGCA GTGATGGCTC TAGTGGTCCT CCCGGGCCAC CTGGCAGCAT TGGTCCCAAA 
GGCCCTGAAG GACTTCAGGG TCAGAAGGGT GAGCGAGGTC CCCCTGGAGA GAGCGTGGTG 
GGTGCCCCTG GGGCCCCTGG AACTCCTGGA GAGAGAGGGG AGCAGGGGCG ACCAGGCCCC 
GCGGGGCCCC GTGGTGAAAA GGGAGAAGCT GCGCTGACGG AGGATGACAT TCGGGGCCTT 
GTGCGTCAGG AGATGAGTCA ACATTGCGCC TGTCAGGGCC AGTATATCGC ATCTGGATCA 
CGGCCTCTCC CGAGTTACGC AGCAGACACC GCTGGCCCGC AGCTCCACCC CGTGCCCGTG 
CTCCGCGTGT CCCACGCAGA GGAGGACGGC CAGGTGCCCC CCGAGGACGA TGAGTATGAA 
TACTCTGAGT ATTCCATGGA GGAATACCAG GACCCCGCGG CTCCTTGGGA TGACGTTGAC 
CCCTGCTCGC TTCCACTGGA CGAGGGTTCC TGCAGTGCCT ACACCCTGCG CTGGTATCAT 
CGGGCTGGGG CGGGGGGCAC GAAGGCCTGT CACCCCTTCG TCTACGGTGG CTGCGGAGGG 
AATGCCAACC GTTTTGGGAC CCGGGAGGCC TGTGAGCACC GCTGCCTGCC CCAGGCGGCC 
CACAGTCAAG GGACAGGTGC TGCCCGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697097.2
CDS	189..8981
Translation	MRLRLLVAALCAGILAGAPRVWAQPRERVSCTRLYAADIVFLLDGSSSIGRSNFREVRGFLEGLVLPFSGAAGAQGVRFAAVQYSDDPRTEFDLDALGSGGDVIRAIRELSYKGGNTRTGAAILHVADRVFLPQLARPGVPKVCILITDGKSQDMVDTAAQRLKGQGVKLFAVGIKNADPEELKRIASQPTSDFFFFVNDFSILRTLLPLVSRRVCTTAGGVPVTLPPDDSTSGPRDLLLEPGSQSLRVQWTAASGPVTGYKVQYAPLTGLGQPLPSELREVSVPAGETSVQLQGLRPLTEYQVTVVALYANSIGEAVSGTARTTALEGPEVTIQNTTAHSLLVAWQSVPGATGYRVTWRVVRGGATQQQELGSGQGSVLLRDLQPGTDYEVTVSALLGRSVGPATSLTARTDTSVEQTLRPIILGPTSILLSWNLVPEARGYRLEWRRESGLEVPQKVVLPSDVTRYQLDGLQPGTEYRLTLYTLLEGREVATPATVVPTELPVGPVTALQATELPGQRVRVSWSPVPGATEYRITVRSAQGVERSLVLPGSQTAFDLDDVRAGLSYTVRVSARVGGREGAASILTVRREPEPPLAIPGLRVMASDATRVRVAWGPVPGASGFRISWRTDNGLESSQTVPSDSTATDIVGLRPGTSYQVAVSALRGREEGPPAVIVARTDPLGPVRSVHVTRVSSSSVTITWNRVPGATGYRVSWRSGRGPEKSQLVSGEATVAELDGLEPDTEYTVRVWARVAGVDGTPASAVVRTDPRPVGSVSKLQIHNASSDVLRVTWVGVPGATAYRLAWGRSEGGPTRQQILPGNTDSAEIRGLEGGVSYSVRVTALIGDREGAPVSIVVTTPPEAPPALETLRVEQRGEHSLRLHWQPVPGARGFRLRWRPEGGQEQSRVLGPEVSSYDLDGLEPATHYRIWLSVLGTAGEGPPSEVTAYTESPRVPSTQLRVVDTSVDSVTLAWTPVSGVSSYILSWRPLRGPGQELPGASQTLPGTSSSQRVTGLELGISYLFSLTPVRDGVRGPEASITQSPACPRGLMDVVFLVHATRDNAHRSEAVKRALEHLVSALGPLGPQAVQVGLLSYSHRPSPLLSLNSSHDLGVILQKIRNIPYTDPSGNNLGTAVVTAHRYLLAPDAPGRRRHVPGVMVLLVDEPLRGDIFNSIREAQAAGLRVMMLGLAGADPEQLRRLVPGMDPSQTFFAVDDGLSLERAVSSLAAALCQTALTTQPQPEPCSVHCPKGQKGEPGQMGLKGQAGPPGPPGLPGRIGAPGPQGPPGSASAKGERGFPGADGPPGSPGRPGTPGAPGPKGSPGWAGPRGEPGERGPRGPKGEPGEPGQVIGGEGPGLPGQKGDPGPPGPPGSRGPLGDPGPRGPPGFPGTAVKGEKGDRGEQGPPGPGDGTAALGEPGLPGLPGSPGPQGSVGPPGEKGEKGDCEDGAPGLPGQPGIPGERGLRGLPGDAGPKGDRGLTGAAGEKGERGSPGPVGPQGLPGVAGRPGPEGPEGPPGPTGRRGEKGEPGRPGDPAVGPGGAGAKGEKGDMGPPGPKGATGFKGEQGSPGLALPGDPGPKGDPGGRGPIGLTGRAGPPGDSGPPGEKGDPGRPGSPGPVGPRGRDGEVGEKGDDGPPGDPGLPGKAGERGLRGAPGARGPVGEKGDQGDPGEEGRNGSPGPSGPKGDRGEPGPPGAPGRLVDVGLGAGEKGEPGDRGQEGPRGPKGDPGPPGASGERGVSGLRGPPGPQGDPGVRGPAGEKGDRGPPGLDGRSGLDGKPGASGPPGPNGAMGKAGDPGRDGLPGLRGEQGPPGPAGPPGAPGKPGEDGKPGLNGKNGEPGDPGEDGRKGEKGDSGAPGREGRDGPKGERGAPGSPGLQGPPGLPGQVGPPGQGFPGVPGSSGPKGDRGETGPRGEQGLPGERGLRGEPGSLVNAERLLENIGIKTSALREIVETWEESSGGFLPTPERRRGPKGDPGERGPPGKEGPIGFSGERGPKGDRGDPGPQGPPGLALGERGLPGPTGLAGEPGKPGIPGLPGPAGSVGEAGRPGERGERGEKGERGEQGRDGLPGLPGPPGPPGPKVAVDEPGSGLSREQGPPGFKGAKGDPGSDGDRGPKGDRGEPGIKGDRGEPGQRGPNGSPGLPGERGVAGPEGKPGLQGPRGTPGPAGGHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLPGPTGAVGLPGPPGPSGLVGPQGAPGLPGQVGETGKPGAPGRDGATGKDGDRGAPGVPGSPGLPGPVGPKGEPGPMGTPGQAVVGPPGAKGEKGAPGALAGDLVGEPGAKGDRGLPGPRGEKGEAGRAGEPGDPGEDGQKGAPGPKGHKGDPGVGVQGPPGPVGPPGLKGDSGPPGSPGPPGIVGFPGQTGPRGELGQPGPSGERGLAGAPGREGAPGPLGPPGPPGSAGVPGVPGLKGDKGDTGAGLPGARGERGEPGVRGEDGRPGLEGPRGLAGPPGIRGERGEKGDAGASGLKGDKGDSAVILGPPGPRGAKGDSGERGPRGIDGDKGPRGDSGEPGEKGTKGEPGDKGSAGLLGARGLMGPKGEPGAAGIPGEPGSPGKDGVPGVRGDKGDVGFMGPRGLKGERGIKGACGLDGEKGDKGEAGPPGRPGLAGRRGELGEPGVPGQAGAPGKEGLIGPKGDRGFDGQPGPKGDQGEKGERGPPGVGGFPGPRGSDGSSGPPGPPGSIGPKGPEGLQGQKGERGPPGESVVGAPGAPGTPGERGEQGRPGPAGPRGEKGEAALTEDDIRGLVRQEMSQHCACQGQYIASGSRPLPSYAADTAGPQLHPVPVLRVSHAEEDGQVPPEDDEYEYSEYSMEEYQDPAAPWDDVDPCSLPLDEGSCSAYTLRWYHRAGAGGTKACHPFVYGGCGGNANRFGTREACEHRCLPQAAHSQGTGAARS

Target ORF information:

RefSeq Version	XM_002697051.5
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VII alpha 1 (COL7A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697051.5

ORF Insert Sequence:

ATGAGGCTGC GGCTCCTGGT GGCTGCGCTC TGCGCCGGGA TCCTGGCAGG GGCTCCCCGA 
GTGTGGGCCC AGCCCAGGGA GAGAGTGAGC TGCACGCGCC TCTACGCCGC TGACATCGTG 
TTCCTCCTTG ACGGCTCCTC GTCCATCGGC CGAAGCAACT TCCGCGAAGT GCGGGGTTTC 
CTTGAGGGGC TGGTGCTGCC CTTCTCCGGG GCAGCTGGGG CACAGGGTGT GCGCTTCGCG 
GCCGTGCAGT ACAGCGACGA CCCACGGACA GAATTTGACC TGGATGCACT TGGCTCAGGC 
GGAGATGTGA TCCGTGCCAT CCGAGAGCTC AGCTACAAGG GGGGCAACAC GCGCACAGGG 
GCCGCGATTC TCCACGTGGC TGACCGCGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GTATCCTCAT CACAGACGGG AAGTCCCAGG ACATGGTGGA CACAGCTGCC 
CAGAGGTTGA AGGGGCAGGG AGTCAAGCTG TTTGCTGTGG GCATCAAGAA TGCCGACCCC 
GAGGAGCTGA AGCGAATTGC CTCACAGCCG ACTAGCGATT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACGCT CCTGCCTCTG GTTTCCCGGA GGGTGTGCAC AACTGCGGGT 
GGTGTGCCCG TGACCCTGCC CCCCGACGAC TCGACCTCTG GTCCACGAGA CCTGCTGCTT 
GAGCCAGGCA GTCAGTCCTT GAGAGTCCAG TGGACAGCGG CCAGTGGCCC CGTGACTGGC 
TACAAGGTCC AGTACGCACC CCTGACAGGG CTGGGACAGC CATTGCCAAG CGAGCTGCGG 
GAGGTGAGCG TCCCAGCTGG TGAGACCAGC GTGCAGCTGC AGGGTCTCCG GCCACTGACT 
GAGTACCAAG TGACGGTGGT CGCCCTCTAC GCCAACAGCA TCGGGGAGGC CGTGAGCGGG 
ACCGCTAGGA CCACTGCTCT GGAGGGGCCA GAAGTGACCA TCCAGAACAC CACAGCCCAC 
AGCCTCCTGG TGGCCTGGCA GAGTGTGCCA GGTGCCACTG GCTACCGGGT GACGTGGCGG 
GTCGTCAGGG GTGGGGCCAC ACAGCAGCAG GAGCTGGGCT CTGGGCAGGG GTCAGTGTTG 
CTGAGGGACC TGCAGCCTGG CACCGACTAC GAGGTGACCG TGAGTGCCCT GCTTGGCCGG 
AGCGTGGGGC CAGCCACCTC CCTGACTGCC CGCACTGACA CGTCCGTCGA GCAGACCCTG 
CGTCCTATCA TCCTGGGCCC CACGTCCATC CTCCTCTCCT GGAACCTGGT GCCTGAGGCC 
CGTGGCTACC GGCTGGAATG GCGGCGTGAG AGCGGCTTAG AGGTGCCACA GAAGGTGGTG 
CTGCCCTCTG ACGTGACCCG CTACCAGTTG GATGGGCTGC AGCCAGGCAC CGAGTACCGC 
CTCACTCTCT ACACGCTGCT AGAGGGCCGT GAGGTGGCCA CCCCAGCGAC CGTGGTCCCC 
ACTGAGCTGC CCGTGGGCCC CGTCACAGCC CTCCAGGCCA CCGAGCTGCC TGGGCAGCGT 
GTGCGAGTGT CCTGGAGCCC AGTTCCCGGC GCCACCGAGT ATCGCATCAC TGTGCGCAGC 
GCCCAGGGGG TTGAGCGGAG CCTGGTGCTT CCTGGGAGTC AGACGGCCTT TGACTTGGAT 
GACGTCCGGG CTGGGCTCAG CTACACAGTG CGGGTGTCAG CGCGAGTAGG CGGCCGGGAG 
GGCGCTGCCA GCATCCTCAC TGTCCGCCGG GAGCCAGAAC CCCCACTGGC CATCCCAGGG 
CTGCGGGTCA TGGCATCGGA CGCAACGCGA GTGAGGGTGG CCTGGGGACC AGTTCCCGGA 
GCCAGCGGAT TTCGGATTAG CTGGAGGACA GACAATGGTC TGGAGTCCAG CCAGACAGTT 
CCTTCAGACT CTACTGCCAC CGACATTGTG GGGCTGAGAC CTGGGACCTC CTACCAGGTG 
GCCGTGTCGG CGCTGCGAGG AAGAGAGGAG GGCCCTCCTG CGGTCATCGT GGCTCGAACC 
GATCCCTTGG GCCCAGTGAG ATCGGTCCAT GTGACTCGAG TCAGCAGCTC ATCTGTCACC 
ATCACCTGGA ACCGGGTTCC TGGTGCCACA GGGTACAGGG TCTCCTGGCG CTCAGGCCGA 
GGCCCAGAGA AATCCCAGTT GGTTTCTGGG GAGGCCACGG TGGCCGAGCT GGATGGACTG 
GAGCCAGATA CTGAGTACAC CGTGCGTGTG TGGGCCCGTG TGGCTGGTGT GGACGGGACG 
CCTGCCTCTG CGGTTGTGAG AACTGACCCT CGGCCCGTGG GCAGTGTGTC GAAGCTGCAG 
ATCCATAATG CTTCCAGTGA CGTTCTGCGG GTCACCTGGG TGGGGGTCCC TGGAGCCACA 
GCCTACAGAC TGGCCTGGGG CCGGAGTGAG GGTGGCCCCA CAAGACAGCA GATTCTCCCA 
GGAAACACGG ACTCCGCAGA AATACGGGGC CTCGAAGGCG GAGTCAGTTA TTCAGTGAGA 
GTGACCGCAC TCATAGGGGA CCGCGAGGGC GCGCCTGTCT CCATTGTCGT CACCACCCCT 
CCGGAGGCTC CTCCAGCCCT GGAGACACTT CGCGTGGAGC AGCGAGGGGA GCACTCACTG 
AGGCTGCACT GGCAGCCGGT GCCTGGTGCA CGCGGCTTCC GTCTGCGTTG GCGACCTGAG 
GGTGGCCAGG AACAGTCCCG AGTCCTGGGG CCGGAAGTCA GCAGCTATGA CCTGGATGGG 
CTGGAGCCAG CGACCCACTA CCGCATATGG CTGAGCGTCT TGGGGACGGC TGGAGAAGGG 
CCCCCCTCGG AAGTGACTGC ATACACTGAG TCACCTCGTG TCCCAAGCAC TCAACTGCGT 
GTGGTGGACA CGTCAGTCGA CTCAGTGACT CTGGCCTGGA CCCCAGTGTC TGGGGTATCC 
AGCTACATCC TATCCTGGCG GCCTCTCAGA GGCCCTGGCC AAGAACTGCC TGGGGCCTCA 
CAGACACTTC CGGGGACCTC AAGTTCCCAG CGGGTGACAG GCCTAGAGCT GGGCATCTCC 
TACCTCTTCT CCCTAACTCC TGTCCGGGAC GGTGTACGCG GTCCTGAAGC CTCTATCACA 
CAGAGCCCAG CGTGTCCCCG CGGCCTGATG GATGTGGTGT TCCTGGTGCA CGCCACTCGA 
GACAATGCTC ATCGCTCGGA GGCTGTGAAG CGAGCCCTGG AGCACCTGGT GTCTGCACTC 
GGGCCTCTCG GGCCACAGGC CGTCCAGGTC GGCCTCCTGT CCTACAGTCA CCGGCCCTCC 
CCACTGTTGT CGCTGAACAG CTCCCATGAC CTTGGCGTCA TCCTGCAGAA GATCCGCAAC 
ATCCCCTACA CGGACCCAAG TGGGAACAAC CTGGGCACAG CCGTGGTTAC AGCCCACAGA 
TACCTGCTGG CACCAGATGC GCCTGGACGC CGCCGGCATG TGCCGGGGGT GATGGTTCTC 
CTGGTGGATG AGCCCTTGAG AGGTGACATC TTCAACTCTA TCCGTGAGGC CCAGGCTGCT 
GGGCTCAGAG TGATGATGCT GGGGCTGGCG GGAGCTGACC CAGAGCAGCT GCGTCGCTTG 
GTGCCTGGCA TGGACCCTTC CCAGACCTTC TTCGCTGTGG ATGATGGTCT GAGCTTGGAG 
CGGGCCGTCA GTAGTCTGGC AGCAGCCCTG TGTCAGACAG CCTTGACCAC GCAGCCACAG 
CCAGAGCCTT GCTCAGTGCA TTGTCCAAAG GGCCAGAAGG GGGAACCCGG ACAGATGGGC 
CTGAAAGGAC AAGCCGGGCC TCCTGGCCCC CCCGGCCTCC CGGGCAGGAT TGGTGCTCCT 
GGCCCCCAGG GACCTCCTGG AAGTGCTTCT GCGAAGGGCG AGAGGGGCTT CCCTGGGGCA 
GATGGGCCTC CAGGCAGCCC TGGCCGCCCT GGGACTCCTG GAGCCCCTGG TCCGAAGGGC 
TCCCCAGGGT GGGCCGGCCC TCGTGGAGAA CCAGGAGAGC GAGGACCTCG AGGCCCAAAG 
GGGGAGCCGG GAGAGCCTGG ACAAGTCATT GGAGGCGAGG GCCCAGGGCT TCCTGGGCAG 
AAAGGGGACC CTGGACCTCC AGGTCCCCCT GGATCTCGTG GGCCGTTGGG GGACCCGGGA 
CCCCGGGGTC CCCCGGGATT TCCTGGAACA GCTGTGAAGG GTGAGAAAGG CGATCGTGGG 
GAGCAGGGCC CCCCTGGACC AGGTGACGGC ACTGCTGCTC TGGGCGAGCC TGGGCTGCCG 
GGTCTTCCTG GAAGCCCCGG ACCCCAAGGC TCAGTTGGCC CCCCTGGAGA GAAAGGAGAA 
AAGGGTGACT GTGAAGATGG AGCCCCGGGC CTCCCAGGAC AACCCGGAAT TCCGGGTGAG 
CGGGGCCTTC GGGGACTTCC TGGAGATGCT GGCCCCAAAG GCGACCGAGG ACTAACAGGG 
GCCGCTGGAG AGAAGGGTGA ACGTGGATCG CCTGGCCCAG TGGGACCACA GGGGCTGCCA 
GGAGTTGCTG GACGACCTGG GCCCGAGGGT CCTGAAGGGC CACCAGGACC GACCGGCCGC 
CGAGGAGAGA AGGGGGAGCC TGGTCGGCCT GGAGACCCTG CAGTGGGACC TGGGGGTGCT 
GGAGCTAAAG GAGAGAAGGG AGATATGGGC CCCCCCGGGC CCAAAGGAGC TACCGGATTC 
AAAGGGGAAC AGGGCTCACC TGGCTTGGCT CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC 
CCTGGAGGCC GAGGTCCCAT TGGCCTCACT GGCAGAGCAG GACCCCCGGG CGACTCAGGA 
CCCCCTGGAG AGAAGGGAGA CCCTGGGCGG CCCGGTTCCC CAGGACCCGT CGGCCCCCGA 
GGACGAGACG GTGAAGTTGG AGAGAAGGGC GATGACGGTC CCCCGGGTGA CCCAGGTTTG 
CCTGGAAAAG CTGGCGAGCG TGGCCTTCGG GGAGCGCCTG GAGCTCGGGG GCCTGTGGGT 
GAGAAGGGAG ACCAGGGAGA TCCTGGAGAG GAAGGACGAA ATGGGAGCCC TGGACCGTCT 
GGACCCAAGG GTGACCGTGG GGAGCCAGGT CCCCCAGGAG CCCCTGGACG GCTGGTGGAC 
GTGGGACTTG GAGCCGGAGA GAAGGGAGAG CCTGGGGACC GAGGACAGGA GGGTCCACGA 
GGACCCAAGG GCGACCCTGG CCCCCCTGGA GCTTCTGGGG AGAGGGGCGT CAGTGGACTT 
CGGGGGCCTC CAGGCCCACA GGGGGACCCA GGTGTTCGAG GCCCAGCAGG AGAAAAGGGC 
GACCGGGGCC CTCCTGGCCT GGATGGCCGC AGTGGGCTGG ATGGGAAACC GGGAGCCTCT 
GGTCCCCCTG GGCCGAATGG TGCCATGGGC AAGGCCGGAG ACCCAGGGAG AGACGGACTT 
CCGGGCCTTC GAGGAGAACA GGGCCCCCCT GGCCCTGCTG GTCCCCCTGG AGCACCGGGA 
AAGCCGGGCG AGGATGGCAA GCCTGGCCTG AATGGGAAAA ACGGAGAACC CGGGGACCCT 
GGAGAAGACG GGAGGAAGGG AGAGAAGGGA GATTCAGGCG CCCCTGGGAG AGAAGGTCGT 
GACGGCCCCA AGGGTGAGCG TGGAGCTCCT GGTAGCCCTG GACTCCAGGG CCCCCCGGGC 
CTCCCAGGGC AGGTCGGCCC TCCCGGGCAG GGCTTCCCTG GCGTCCCGGG GAGTTCAGGC 
CCCAAGGGTG ACCGTGGGGA GACTGGACCC AGAGGGGAGC AGGGCCTCCC AGGAGAGCGT 
GGCCTGAGAG GAGAGCCTGG GAGCCTGGTG AATGCAGAGC GGTTGCTGGA AAATATTGGC 
ATCAAGACAT CTGCCCTGAG GGAGATCGTG GAGACCTGGG AAGAAAGCTC TGGTGGCTTC 
CTGCCCACGC CTGAACGGCG TCGCGGCCCC AAGGGGGACC CAGGCGAGCG GGGCCCCCCA 
GGCAAGGAGG GCCCCATCGG CTTTTCTGGA GAACGTGGAC CAAAGGGAGA TCGTGGAGAC 
CCCGGCCCTC AGGGGCCACC TGGCCTGGCC CTGGGGGAGA GGGGCCTTCC TGGACCTACC 
GGCCTTGCCG GGGAGCCTGG GAAGCCTGGT ATCCCTGGGC TCCCTGGCCC AGCTGGGAGT 
GTGGGGGAGG CCGGAAGACC CGGAGAGAGG GGAGAACGGG GAGAGAAAGG AGAACGTGGA 
GAACAGGGCA GAGACGGCCT TCCTGGCCTC CCTGGACCCC CCGGACCCCC AGGCCCCAAG 
GTGGCCGTGG ACGAGCCAGG TTCTGGGCTC TCTAGAGAAC AAGGACCCCC TGGATTCAAG 
GGTGCTAAGG GGGATCCAGG CAGTGATGGC GACCGAGGCC CCAAAGGAGA CAGGGGTGAG 
CCGGGCATCA AGGGCGACCG GGGAGAGCCT GGACAGAGGG GTCCGAATGG CAGCCCGGGT 
CTGCCAGGAG AGCGCGGCGT GGCTGGGCCC GAGGGGAAGC CGGGTCTGCA AGGTCCACGG 
GGGACCCCTG GCCCGGCGGG CGGCCATGGA GACCCTGGAC CACCCGGGGC CCCGGGTCTT 
GCTGGCCCTG CGGGACCCCA GGGACCTTCT GGCCTAAAGG GGGAGCCCGG AGAGACAGGA 
CCACCAGGAC GGGGCCTGCC TGGACCTACT GGAGCCGTGG GACTTCCCGG GCCCCCTGGC 
CCTTCAGGCC TGGTGGGTCC TCAAGGGGCA CCGGGTTTGC CTGGACAAGT GGGGGAGACG 
GGAAAGCCGG GAGCGCCAGG TCGTGATGGT GCCACTGGGA AAGATGGAGA CAGAGGAGCC 
CCCGGTGTGC CGGGGTCACC AGGTCTGCCA GGCCCTGTCG GACCTAAAGG AGAGCCGGGA 
CCCATGGGGA CCCCTGGACA GGCTGTGGTC GGGCCCCCTG GAGCAAAGGG AGAGAAGGGC 
GCCCCTGGAG CCCTTGCTGG AGACCTGGTG GGAGAGCCGG GAGCCAAGGG TGACCGAGGA 
CTGCCAGGGC CGCGGGGCGA GAAGGGTGAA GCTGGCCGTG CGGGGGAGCC TGGAGACCCT 
GGCGAAGATG GTCAGAAGGG GGCTCCGGGA CCCAAAGGTC ACAAGGGTGA CCCAGGAGTT 
GGGGTCCAGG GGCCCCCTGG GCCAGTTGGC CCTCCAGGTC TAAAGGGAGA CTCAGGGCCC 
CCCGGATCCC CCGGACCTCC TGGTATTGTG GGGTTCCCCG GTCAGACAGG CCCTCGAGGA 
GAGCTGGGGC AGCCGGGCCC CAGTGGAGAG CGGGGTCTGG CAGGTGCCCC AGGGAGAGAG 
GGAGCCCCAG GTCCCTTGGG GCCGCCTGGA CCACCTGGGT CAGCGGGAGT ACCTGGGGTC 
CCTGGACTCA AAGGAGACAA GGGTGACACT GGAGCAGGGC TGCCTGGGGC CCGAGGCGAG 
CGTGGGGAGC CCGGTGTCCG GGGTGAAGAT GGCCGCCCCG GCCTGGAGGG ACCCCGAGGA 
CTTGCGGGTC CCCCAGGCAT CCGGGGAGAA CGTGGGGAGA AGGGTGACGC TGGTGCCTCA 
GGACTGAAGG GTGACAAGGG CGACTCAGCT GTGATTTTGG GGCCTCCGGG GCCACGGGGT 
GCCAAGGGGG ACTCGGGTGA ACGAGGGCCT CGGGGAATAG ATGGTGACAA AGGACCTCGG 
GGAGACAGCG GGGAACCTGG AGAGAAGGGC ACCAAGGGAG AGCCTGGTGA CAAGGGCTCA 
GCCGGGCTGC TGGGAGCGCG CGGACTCATG GGACCCAAGG GCGAGCCTGG TGCCGCAGGG 
ATCCCCGGCG AACCGGGATC CCCAGGAAAA GACGGAGTCC CTGGTGTCCG AGGAGACAAA 
GGAGATGTTG GCTTCATGGG TCCCCGGGGC CTCAAGGGTG AACGGGGAAT TAAGGGAGCC 
TGTGGCCTCG ACGGAGAGAA GGGAGATAAG GGAGAAGCCG GCCCCCCGGG CCGCCCAGGG 
CTGGCAGGAC GCAGAGGAGA GCTGGGGGAG CCGGGTGTTC CAGGCCAGGC AGGGGCCCCT 
GGGAAGGAGG GCCTGATCGG TCCCAAGGGT GACCGAGGTT TCGATGGGCA GCCAGGACCC 
AAGGGTGACC AGGGCGAGAA AGGGGAGCGG GGACCCCCAG GAGTGGGGGG CTTCCCAGGT 
CCCAGGGGCA GTGATGGCTC TAGTGGTCCT CCCGGGCCAC CTGGCAGCAT TGGTCCCAAA 
GGCCCTGAAG GACTTCAGGG TCAGAAGGGT GAGCGAGGTC CCCCTGGAGA GAGCGTGGTG 
GGTGCCCCTG GGGCCCCTGG AACTCCTGGA GAGAGAGGGG AGCAGGGGCG ACCAGGCCCC 
GCGGGGCCCC GTGGTGAAAA GGGAGAAGCT GCGCTGACGG AGGATGACAT TCGGGGCCTT 
GTGCGTCAGG AGATGAGTCA ACATTGCGCC TGTCAGGGCC AGTATATCGC ATCTGGATCA 
CGGCCTCTCC CGAGTTACGC AGCAGACACC GCTGGCCCGC AGCTCCACCC CGTGCCCGTG 
CTCCGCGTGT CCCACGCAGA GGAGGACGGC CAGGTGCCCC CCGAGGACGA TGAGTATGAA 
TACTCTGAGT ATTCCATGGA GGAATACCAG GACCCCGCGG CTCCTTGGGA TGACGTTGAC 
CCCTGCTCGC TTCCACTGGA CGAGGGTTCC TGCAGTGCCT ACACCCTGCG CTGGTATCAT 
CGGGCTGGGG CGGGGGGCAC GAAGGCCTGT CACCCCTTCG TCTACGGTGG CTGCGGAGGG 
AATGCCAACC GTTTTGGGAC CCGGGAGGCC TGTGAGCACC GCTGCCTGCC CCAGGCGGCC 
CACAGTCAAG GGACAGGTGC TGCCCGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL7A1 ( XM_015468790.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324276.1 Bos taurus collagen type VII alpha 1 (COL7A1), mRNA.

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CloneID	OBa31702
Clone ID Related Accession (Same CDS sequence)	XM_002697051.5 , XM_015468790.1
Accession Version	XM_015468790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	collagen alpha-1(VII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTGC GGCTCCTGGT GGCTGCGCTC TGCGCCGGGA TCCTGGCAGG GGCTCCCCGA 
GTGTGGGCCC AGCCCAGGGA GAGAGTGAGC TGCACGCGCC TCTACGCCGC TGACATCGTG 
TTCCTCCTTG ACGGCTCCTC GTCCATCGGC CGAAGCAACT TCCGCGAAGT GCGGGGTTTC 
CTTGAGGGGC TGGTGCTGCC CTTCTCCGGG GCAGCTGGGG CACAGGGTGT GCGCTTCGCG 
GCCGTGCAGT ACAGCGACGA CCCACGGACA GAATTTGACC TGGATGCACT TGGCTCAGGC 
GGAGATGTGA TCCGTGCCAT CCGAGAGCTC AGCTACAAGG GGGGCAACAC GCGCACAGGG 
GCCGCGATTC TCCACGTGGC TGACCGCGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GTATCCTCAT CACAGACGGG AAGTCCCAGG ACATGGTGGA CACAGCTGCC 
CAGAGGTTGA AGGGGCAGGG AGTCAAGCTG TTTGCTGTGG GCATCAAGAA TGCCGACCCC 
GAGGAGCTGA AGCGAATTGC CTCACAGCCG ACTAGCGATT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACGCT CCTGCCTCTG GTTTCCCGGA GGGTGTGCAC AACTGCGGGT 
GGTGTGCCCG TGACCCTGCC CCCCGACGAC TCGACCTCTG GTCCACGAGA CCTGCTGCTT 
GAGCCAGGCA GTCAGTCCTT GAGAGTCCAG TGGACAGCGG CCAGTGGCCC CGTGACTGGC 
TACAAGGTCC AGTACGCACC CCTGACAGGG CTGGGACAGC CATTGCCAAG CGAGCTGCGG 
GAGGTGAGCG TCCCAGCTGG TGAGACCAGC GTGCAGCTGC AGGGTCTCCG GCCACTGACT 
GAGTACCAAG TGACGGTGGT CGCCCTCTAC GCCAACAGCA TCGGGGAGGC CGTGAGCGGG 
ACCGCTAGGA CCACTGCTCT GGAGGGGCCA GAAGTGACCA TCCAGAACAC CACAGCCCAC 
AGCCTCCTGG TGGCCTGGCA GAGTGTGCCA GGTGCCACTG GCTACCGGGT GACGTGGCGG 
GTCGTCAGGG GTGGGGCCAC ACAGCAGCAG GAGCTGGGCT CTGGGCAGGG GTCAGTGTTG 
CTGAGGGACC TGCAGCCTGG CACCGACTAC GAGGTGACCG TGAGTGCCCT GCTTGGCCGG 
AGCGTGGGGC CAGCCACCTC CCTGACTGCC CGCACTGACA CGTCCGTCGA GCAGACCCTG 
CGTCCTATCA TCCTGGGCCC CACGTCCATC CTCCTCTCCT GGAACCTGGT GCCTGAGGCC 
CGTGGCTACC GGCTGGAATG GCGGCGTGAG AGCGGCTTAG AGGTGCCACA GAAGGTGGTG 
CTGCCCTCTG ACGTGACCCG CTACCAGTTG GATGGGCTGC AGCCAGGCAC CGAGTACCGC 
CTCACTCTCT ACACGCTGCT AGAGGGCCGT GAGGTGGCCA CCCCAGCGAC CGTGGTCCCC 
ACTGAGCTGC CCGTGGGCCC CGTCACAGCC CTCCAGGCCA CCGAGCTGCC TGGGCAGCGT 
GTGCGAGTGT CCTGGAGCCC AGTTCCCGGC GCCACCGAGT ATCGCATCAC TGTGCGCAGC 
GCCCAGGGGG TTGAGCGGAG CCTGGTGCTT CCTGGGAGTC AGACGGCCTT TGACTTGGAT 
GACGTCCGGG CTGGGCTCAG CTACACAGTG CGGGTGTCAG CGCGAGTAGG CGGCCGGGAG 
GGCGCTGCCA GCATCCTCAC TGTCCGCCGG GAGCCAGAAC CCCCACTGGC CATCCCAGGG 
CTGCGGGTCA TGGCATCGGA CGCAACGCGA GTGAGGGTGG CCTGGGGACC AGTTCCCGGA 
GCCAGCGGAT TTCGGATTAG CTGGAGGACA GACAATGGTC TGGAGTCCAG CCAGACAGTT 
CCTTCAGACT CTACTGCCAC CGACATTGTG GGGCTGAGAC CTGGGACCTC CTACCAGGTG 
GCCGTGTCGG CGCTGCGAGG AAGAGAGGAG GGCCCTCCTG CGGTCATCGT GGCTCGAACC 
GATCCCTTGG GCCCAGTGAG ATCGGTCCAT GTGACTCGAG TCAGCAGCTC ATCTGTCACC 
ATCACCTGGA ACCGGGTTCC TGGTGCCACA GGGTACAGGG TCTCCTGGCG CTCAGGCCGA 
GGCCCAGAGA AATCCCAGTT GGTTTCTGGG GAGGCCACGG TGGCCGAGCT GGATGGACTG 
GAGCCAGATA CTGAGTACAC CGTGCGTGTG TGGGCCCGTG TGGCTGGTGT GGACGGGACG 
CCTGCCTCTG CGGTTGTGAG AACTGACCCT CGGCCCGTGG GCAGTGTGTC GAAGCTGCAG 
ATCCATAATG CTTCCAGTGA CGTTCTGCGG GTCACCTGGG TGGGGGTCCC TGGAGCCACA 
GCCTACAGAC TGGCCTGGGG CCGGAGTGAG GGTGGCCCCA CAAGACAGCA GATTCTCCCA 
GGAAACACGG ACTCCGCAGA AATACGGGGC CTCGAAGGCG GAGTCAGTTA TTCAGTGAGA 
GTGACCGCAC TCATAGGGGA CCGCGAGGGC GCGCCTGTCT CCATTGTCGT CACCACCCCT 
CCGGAGGCTC CTCCAGCCCT GGAGACACTT CGCGTGGAGC AGCGAGGGGA GCACTCACTG 
AGGCTGCACT GGCAGCCGGT GCCTGGTGCA CGCGGCTTCC GTCTGCGTTG GCGACCTGAG 
GGTGGCCAGG AACAGTCCCG AGTCCTGGGG CCGGAAGTCA GCAGCTATGA CCTGGATGGG 
CTGGAGCCAG CGACCCACTA CCGCATATGG CTGAGCGTCT TGGGGACGGC TGGAGAAGGG 
CCCCCCTCGG AAGTGACTGC ATACACTGAG TCACCTCGTG TCCCAAGCAC TCAACTGCGT 
GTGGTGGACA CGTCAGTCGA CTCAGTGACT CTGGCCTGGA CCCCAGTGTC TGGGGTATCC 
AGCTACATCC TATCCTGGCG GCCTCTCAGA GGCCCTGGCC AAGAACTGCC TGGGGCCTCA 
CAGACACTTC CGGGGACCTC AAGTTCCCAG CGGGTGACAG GCCTAGAGCT GGGCATCTCC 
TACCTCTTCT CCCTAACTCC TGTCCGGGAC GGTGTACGCG GTCCTGAAGC CTCTATCACA 
CAGAGCCCAG CGTGTCCCCG CGGCCTGATG GATGTGGTGT TCCTGGTGCA CGCCACTCGA 
GACAATGCTC ATCGCTCGGA GGCTGTGAAG CGAGCCCTGG AGCACCTGGT GTCTGCACTC 
GGGCCTCTCG GGCCACAGGC CGTCCAGGTC GGCCTCCTGT CCTACAGTCA CCGGCCCTCC 
CCACTGTTGT CGCTGAACAG CTCCCATGAC CTTGGCGTCA TCCTGCAGAA GATCCGCAAC 
ATCCCCTACA CGGACCCAAG TGGGAACAAC CTGGGCACAG CCGTGGTTAC AGCCCACAGA 
TACCTGCTGG CACCAGATGC GCCTGGACGC CGCCGGCATG TGCCGGGGGT GATGGTTCTC 
CTGGTGGATG AGCCCTTGAG AGGTGACATC TTCAACTCTA TCCGTGAGGC CCAGGCTGCT 
GGGCTCAGAG TGATGATGCT GGGGCTGGCG GGAGCTGACC CAGAGCAGCT GCGTCGCTTG 
GTGCCTGGCA TGGACCCTTC CCAGACCTTC TTCGCTGTGG ATGATGGTCT GAGCTTGGAG 
CGGGCCGTCA GTAGTCTGGC AGCAGCCCTG TGTCAGACAG CCTTGACCAC GCAGCCACAG 
CCAGAGCCTT GCTCAGTGCA TTGTCCAAAG GGCCAGAAGG GGGAACCCGG ACAGATGGGC 
CTGAAAGGAC AAGCCGGGCC TCCTGGCCCC CCCGGCCTCC CGGGCAGGAT TGGTGCTCCT 
GGCCCCCAGG GACCTCCTGG AAGTGCTTCT GCGAAGGGCG AGAGGGGCTT CCCTGGGGCA 
GATGGGCCTC CAGGCAGCCC TGGCCGCCCT GGGACTCCTG GAGCCCCTGG TCCGAAGGGC 
TCCCCAGGGT GGGCCGGCCC TCGTGGAGAA CCAGGAGAGC GAGGACCTCG AGGCCCAAAG 
GGGGAGCCGG GAGAGCCTGG ACAAGTCATT GGAGGCGAGG GCCCAGGGCT TCCTGGGCAG 
AAAGGGGACC CTGGACCTCC AGGTCCCCCT GGATCTCGTG GGCCGTTGGG GGACCCGGGA 
CCCCGGGGTC CCCCGGGATT TCCTGGAACA GCTGTGAAGG GTGAGAAAGG CGATCGTGGG 
GAGCAGGGCC CCCCTGGACC AGGTGACGGC ACTGCTGCTC TGGGCGAGCC TGGGCTGCCG 
GGTCTTCCTG GAAGCCCCGG ACCCCAAGGC TCAGTTGGCC CCCCTGGAGA GAAAGGAGAA 
AAGGGTGACT GTGAAGATGG AGCCCCGGGC CTCCCAGGAC AACCCGGAAT TCCGGGTGAG 
CGGGGCCTTC GGGGACTTCC TGGAGATGCT GGCCCCAAAG GCGACCGAGG ACTAACAGGG 
GCCGCTGGAG AGAAGGGTGA ACGTGGATCG CCTGGCCCAG TGGGACCACA GGGGCTGCCA 
GGAGTTGCTG GACGACCTGG GCCCGAGGGT CCTGAAGGGC CACCAGGACC GACCGGCCGC 
CGAGGAGAGA AGGGGGAGCC TGGTCGGCCT GGAGACCCTG CAGTGGGACC TGGGGGTGCT 
GGAGCTAAAG GAGAGAAGGG AGATATGGGC CCCCCCGGGC CCAAAGGAGC TACCGGATTC 
AAAGGGGAAC AGGGCTCACC TGGCTTGGCT CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC 
CCTGGAGGCC GAGGTCCCAT TGGCCTCACT GGCAGAGCAG GACCCCCGGG CGACTCAGGA 
CCCCCTGGAG AGAAGGGAGA CCCTGGGCGG CCCGGTTCCC CAGGACCCGT CGGCCCCCGA 
GGACGAGACG GTGAAGTTGG AGAGAAGGGC GATGACGGTC CCCCGGGTGA CCCAGGTTTG 
CCTGGAAAAG CTGGCGAGCG TGGCCTTCGG GGAGCGCCTG GAGCTCGGGG GCCTGTGGGT 
GAGAAGGGAG ACCAGGGAGA TCCTGGAGAG GAAGGACGAA ATGGGAGCCC TGGACCGTCT 
GGACCCAAGG GTGACCGTGG GGAGCCAGGT CCCCCAGGAG CCCCTGGACG GCTGGTGGAC 
GTGGGACTTG GAGCCGGAGA GAAGGGAGAG CCTGGGGACC GAGGACAGGA GGGTCCACGA 
GGACCCAAGG GCGACCCTGG CCCCCCTGGA GCTTCTGGGG AGAGGGGCGT CAGTGGACTT 
CGGGGGCCTC CAGGCCCACA GGGGGACCCA GGTGTTCGAG GCCCAGCAGG AGAAAAGGGC 
GACCGGGGCC CTCCTGGCCT GGATGGCCGC AGTGGGCTGG ATGGGAAACC GGGAGCCTCT 
GGTCCCCCTG GGCCGAATGG TGCCATGGGC AAGGCCGGAG ACCCAGGGAG AGACGGACTT 
CCGGGCCTTC GAGGAGAACA GGGCCCCCCT GGCCCTGCTG GTCCCCCTGG AGCACCGGGA 
AAGCCGGGCG AGGATGGCAA GCCTGGCCTG AATGGGAAAA ACGGAGAACC CGGGGACCCT 
GGAGAAGACG GGAGGAAGGG AGAGAAGGGA GATTCAGGCG CCCCTGGGAG AGAAGGTCGT 
GACGGCCCCA AGGGTGAGCG TGGAGCTCCT GGTAGCCCTG GACTCCAGGG CCCCCCGGGC 
CTCCCAGGGC AGGTCGGCCC TCCCGGGCAG GGCTTCCCTG GCGTCCCGGG GAGTTCAGGC 
CCCAAGGGTG ACCGTGGGGA GACTGGACCC AGAGGGGAGC AGGGCCTCCC AGGAGAGCGT 
GGCCTGAGAG GAGAGCCTGG GAGCCTGGTG AATGCAGAGC GGTTGCTGGA AAATATTGGC 
ATCAAGACAT CTGCCCTGAG GGAGATCGTG GAGACCTGGG AAGAAAGCTC TGGTGGCTTC 
CTGCCCACGC CTGAACGGCG TCGCGGCCCC AAGGGGGACC CAGGCGAGCG GGGCCCCCCA 
GGCAAGGAGG GCCCCATCGG CTTTTCTGGA GAACGTGGAC CAAAGGGAGA TCGTGGAGAC 
CCCGGCCCTC AGGGGCCACC TGGCCTGGCC CTGGGGGAGA GGGGCCTTCC TGGACCTACC 
GGCCTTGCCG GGGAGCCTGG GAAGCCTGGT ATCCCTGGGC TCCCTGGCCC AGCTGGGAGT 
GTGGGGGAGG CCGGAAGACC CGGAGAGAGG GGAGAACGGG GAGAGAAAGG AGAACGTGGA 
GAACAGGGCA GAGACGGCCT TCCTGGCCTC CCTGGACCCC CCGGACCCCC AGGCCCCAAG 
GTGGCCGTGG ACGAGCCAGG TTCTGGGCTC TCTAGAGAAC AAGGACCCCC TGGATTCAAG 
GGTGCTAAGG GGGATCCAGG CAGTGATGGC GACCGAGGCC CCAAAGGAGA CAGGGGTGAG 
CCGGGCATCA AGGGCGACCG GGGAGAGCCT GGACAGAGGG GTCCGAATGG CAGCCCGGGT 
CTGCCAGGAG AGCGCGGCGT GGCTGGGCCC GAGGGGAAGC CGGGTCTGCA AGGTCCACGG 
GGGACCCCTG GCCCGGCGGG CGGCCATGGA GACCCTGGAC CACCCGGGGC CCCGGGTCTT 
GCTGGCCCTG CGGGACCCCA GGGACCTTCT GGCCTAAAGG GGGAGCCCGG AGAGACAGGA 
CCACCAGGAC GGGGCCTGCC TGGACCTACT GGAGCCGTGG GACTTCCCGG GCCCCCTGGC 
CCTTCAGGCC TGGTGGGTCC TCAAGGGGCA CCGGGTTTGC CTGGACAAGT GGGGGAGACG 
GGAAAGCCGG GAGCGCCAGG TCGTGATGGT GCCACTGGGA AAGATGGAGA CAGAGGAGCC 
CCCGGTGTGC CGGGGTCACC AGGTCTGCCA GGCCCTGTCG GACCTAAAGG AGAGCCGGGA 
CCCATGGGGA CCCCTGGACA GGCTGTGGTC GGGCCCCCTG GAGCAAAGGG AGAGAAGGGC 
GCCCCTGGAG CCCTTGCTGG AGACCTGGTG GGAGAGCCGG GAGCCAAGGG TGACCGAGGA 
CTGCCAGGGC CGCGGGGCGA GAAGGGTGAA GCTGGCCGTG CGGGGGAGCC TGGAGACCCT 
GGCGAAGATG GTCAGAAGGG GGCTCCGGGA CCCAAAGGTC ACAAGGGTGA CCCAGGAGTT 
GGGGTCCAGG GGCCCCCTGG GCCAGTTGGC CCTCCAGGTC TAAAGGGAGA CTCAGGGCCC 
CCCGGATCCC CCGGACCTCC TGGTATTGTG GGGTTCCCCG GTCAGACAGG CCCTCGAGGA 
GAGCTGGGGC AGCCGGGCCC CAGTGGAGAG CGGGGTCTGG CAGGTGCCCC AGGGAGAGAG 
GGAGCCCCAG GTCCCTTGGG GCCGCCTGGA CCACCTGGGT CAGCGGGAGT ACCTGGGGTC 
CCTGGACTCA AAGGAGACAA GGGTGACACT GGAGCAGGGC TGCCTGGGGC CCGAGGCGAG 
CGTGGGGAGC CCGGTGTCCG GGGTGAAGAT GGCCGCCCCG GCCTGGAGGG ACCCCGAGGA 
CTTGCGGGTC CCCCAGGCAT CCGGGGAGAA CGTGGGGAGA AGGGTGACGC TGGTGCCTCA 
GGACTGAAGG GTGACAAGGG CGACTCAGCT GTGATTTTGG GGCCTCCGGG GCCACGGGGT 
GCCAAGGGGG ACTCGGGTGA ACGAGGGCCT CGGGGAATAG ATGGTGACAA AGGACCTCGG 
GGAGACAGCG GGGAACCTGG AGAGAAGGGC ACCAAGGGAG AGCCTGGTGA CAAGGGCTCA 
GCCGGGCTGC TGGGAGCGCG CGGACTCATG GGACCCAAGG GCGAGCCTGG TGCCGCAGGG 
ATCCCCGGCG AACCGGGATC CCCAGGAAAA GACGGAGTCC CTGGTGTCCG AGGAGACAAA 
GGAGATGTTG GCTTCATGGG TCCCCGGGGC CTCAAGGGTG AACGGGGAAT TAAGGGAGCC 
TGTGGCCTCG ACGGAGAGAA GGGAGATAAG GGAGAAGCCG GCCCCCCGGG CCGCCCAGGG 
CTGGCAGGAC GCAGAGGAGA GCTGGGGGAG CCGGGTGTTC CAGGCCAGGC AGGGGCCCCT 
GGGAAGGAGG GCCTGATCGG TCCCAAGGGT GACCGAGGTT TCGATGGGCA GCCAGGACCC 
AAGGGTGACC AGGGCGAGAA AGGGGAGCGG GGACCCCCAG GAGTGGGGGG CTTCCCAGGT 
CCCAGGGGCA GTGATGGCTC TAGTGGTCCT CCCGGGCCAC CTGGCAGCAT TGGTCCCAAA 
GGCCCTGAAG GACTTCAGGG TCAGAAGGGT GAGCGAGGTC CCCCTGGAGA GAGCGTGGTG 
GGTGCCCCTG GGGCCCCTGG AACTCCTGGA GAGAGAGGGG AGCAGGGGCG ACCAGGCCCC 
GCGGGGCCCC GTGGTGAAAA GGGAGAAGCT GCGCTGACGG AGGATGACAT TCGGGGCCTT 
GTGCGTCAGG AGATGAGTCA ACATTGCGCC TGTCAGGGCC AGTATATCGC ATCTGGATCA 
CGGCCTCTCC CGAGTTACGC AGCAGACACC GCTGGCCCGC AGCTCCACCC CGTGCCCGTG 
CTCCGCGTGT CCCACGCAGA GGAGGACGGC CAGGTGCCCC CCGAGGACGA TGAGTATGAA 
TACTCTGAGT ATTCCATGGA GGAATACCAG GACCCCGCGG CTCCTTGGGA TGACGTTGAC 
CCCTGCTCGC TTCCACTGGA CGAGGGTTCC TGCAGTGCCT ACACCCTGCG CTGGTATCAT 
CGGGCTGGGG CGGGGGGCAC GAAGGCCTGT CACCCCTTCG TCTACGGTGG CTGCGGAGGG 
AATGCCAACC GTTTTGGGAC CCGGGAGGCC TGTGAGCACC GCTGCCTGCC CCAGGCGGCC 
CACAGTCAAG GGACAGGTGC TGCCCGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324276.1
CDS	189..8981
Translation	MRLRLLVAALCAGILAGAPRVWAQPRERVSCTRLYAADIVFLLDGSSSIGRSNFREVRGFLEGLVLPFSGAAGAQGVRFAAVQYSDDPRTEFDLDALGSGGDVIRAIRELSYKGGNTRTGAAILHVADRVFLPQLARPGVPKVCILITDGKSQDMVDTAAQRLKGQGVKLFAVGIKNADPEELKRIASQPTSDFFFFVNDFSILRTLLPLVSRRVCTTAGGVPVTLPPDDSTSGPRDLLLEPGSQSLRVQWTAASGPVTGYKVQYAPLTGLGQPLPSELREVSVPAGETSVQLQGLRPLTEYQVTVVALYANSIGEAVSGTARTTALEGPEVTIQNTTAHSLLVAWQSVPGATGYRVTWRVVRGGATQQQELGSGQGSVLLRDLQPGTDYEVTVSALLGRSVGPATSLTARTDTSVEQTLRPIILGPTSILLSWNLVPEARGYRLEWRRESGLEVPQKVVLPSDVTRYQLDGLQPGTEYRLTLYTLLEGREVATPATVVPTELPVGPVTALQATELPGQRVRVSWSPVPGATEYRITVRSAQGVERSLVLPGSQTAFDLDDVRAGLSYTVRVSARVGGREGAASILTVRREPEPPLAIPGLRVMASDATRVRVAWGPVPGASGFRISWRTDNGLESSQTVPSDSTATDIVGLRPGTSYQVAVSALRGREEGPPAVIVARTDPLGPVRSVHVTRVSSSSVTITWNRVPGATGYRVSWRSGRGPEKSQLVSGEATVAELDGLEPDTEYTVRVWARVAGVDGTPASAVVRTDPRPVGSVSKLQIHNASSDVLRVTWVGVPGATAYRLAWGRSEGGPTRQQILPGNTDSAEIRGLEGGVSYSVRVTALIGDREGAPVSIVVTTPPEAPPALETLRVEQRGEHSLRLHWQPVPGARGFRLRWRPEGGQEQSRVLGPEVSSYDLDGLEPATHYRIWLSVLGTAGEGPPSEVTAYTESPRVPSTQLRVVDTSVDSVTLAWTPVSGVSSYILSWRPLRGPGQELPGASQTLPGTSSSQRVTGLELGISYLFSLTPVRDGVRGPEASITQSPACPRGLMDVVFLVHATRDNAHRSEAVKRALEHLVSALGPLGPQAVQVGLLSYSHRPSPLLSLNSSHDLGVILQKIRNIPYTDPSGNNLGTAVVTAHRYLLAPDAPGRRRHVPGVMVLLVDEPLRGDIFNSIREAQAAGLRVMMLGLAGADPEQLRRLVPGMDPSQTFFAVDDGLSLERAVSSLAAALCQTALTTQPQPEPCSVHCPKGQKGEPGQMGLKGQAGPPGPPGLPGRIGAPGPQGPPGSASAKGERGFPGADGPPGSPGRPGTPGAPGPKGSPGWAGPRGEPGERGPRGPKGEPGEPGQVIGGEGPGLPGQKGDPGPPGPPGSRGPLGDPGPRGPPGFPGTAVKGEKGDRGEQGPPGPGDGTAALGEPGLPGLPGSPGPQGSVGPPGEKGEKGDCEDGAPGLPGQPGIPGERGLRGLPGDAGPKGDRGLTGAAGEKGERGSPGPVGPQGLPGVAGRPGPEGPEGPPGPTGRRGEKGEPGRPGDPAVGPGGAGAKGEKGDMGPPGPKGATGFKGEQGSPGLALPGDPGPKGDPGGRGPIGLTGRAGPPGDSGPPGEKGDPGRPGSPGPVGPRGRDGEVGEKGDDGPPGDPGLPGKAGERGLRGAPGARGPVGEKGDQGDPGEEGRNGSPGPSGPKGDRGEPGPPGAPGRLVDVGLGAGEKGEPGDRGQEGPRGPKGDPGPPGASGERGVSGLRGPPGPQGDPGVRGPAGEKGDRGPPGLDGRSGLDGKPGASGPPGPNGAMGKAGDPGRDGLPGLRGEQGPPGPAGPPGAPGKPGEDGKPGLNGKNGEPGDPGEDGRKGEKGDSGAPGREGRDGPKGERGAPGSPGLQGPPGLPGQVGPPGQGFPGVPGSSGPKGDRGETGPRGEQGLPGERGLRGEPGSLVNAERLLENIGIKTSALREIVETWEESSGGFLPTPERRRGPKGDPGERGPPGKEGPIGFSGERGPKGDRGDPGPQGPPGLALGERGLPGPTGLAGEPGKPGIPGLPGPAGSVGEAGRPGERGERGEKGERGEQGRDGLPGLPGPPGPPGPKVAVDEPGSGLSREQGPPGFKGAKGDPGSDGDRGPKGDRGEPGIKGDRGEPGQRGPNGSPGLPGERGVAGPEGKPGLQGPRGTPGPAGGHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLPGPTGAVGLPGPPGPSGLVGPQGAPGLPGQVGETGKPGAPGRDGATGKDGDRGAPGVPGSPGLPGPVGPKGEPGPMGTPGQAVVGPPGAKGEKGAPGALAGDLVGEPGAKGDRGLPGPRGEKGEAGRAGEPGDPGEDGQKGAPGPKGHKGDPGVGVQGPPGPVGPPGLKGDSGPPGSPGPPGIVGFPGQTGPRGELGQPGPSGERGLAGAPGREGAPGPLGPPGPPGSAGVPGVPGLKGDKGDTGAGLPGARGERGEPGVRGEDGRPGLEGPRGLAGPPGIRGERGEKGDAGASGLKGDKGDSAVILGPPGPRGAKGDSGERGPRGIDGDKGPRGDSGEPGEKGTKGEPGDKGSAGLLGARGLMGPKGEPGAAGIPGEPGSPGKDGVPGVRGDKGDVGFMGPRGLKGERGIKGACGLDGEKGDKGEAGPPGRPGLAGRRGELGEPGVPGQAGAPGKEGLIGPKGDRGFDGQPGPKGDQGEKGERGPPGVGGFPGPRGSDGSSGPPGPPGSIGPKGPEGLQGQKGERGPPGESVVGAPGAPGTPGERGEQGRPGPAGPRGEKGEAALTEDDIRGLVRQEMSQHCACQGQYIASGSRPLPSYAADTAGPQLHPVPVLRVSHAEEDGQVPPEDDEYEYSEYSMEEYQDPAAPWDDVDPCSLPLDEGSCSAYTLRWYHRAGAGGTKACHPFVYGGCGGNANRFGTREACEHRCLPQAAHSQGTGAARS

Target ORF information:

RefSeq Version	XM_015468790.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VII alpha 1 (COL7A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468790.1

ORF Insert Sequence:

ATGAGGCTGC GGCTCCTGGT GGCTGCGCTC TGCGCCGGGA TCCTGGCAGG GGCTCCCCGA 
GTGTGGGCCC AGCCCAGGGA GAGAGTGAGC TGCACGCGCC TCTACGCCGC TGACATCGTG 
TTCCTCCTTG ACGGCTCCTC GTCCATCGGC CGAAGCAACT TCCGCGAAGT GCGGGGTTTC 
CTTGAGGGGC TGGTGCTGCC CTTCTCCGGG GCAGCTGGGG CACAGGGTGT GCGCTTCGCG 
GCCGTGCAGT ACAGCGACGA CCCACGGACA GAATTTGACC TGGATGCACT TGGCTCAGGC 
GGAGATGTGA TCCGTGCCAT CCGAGAGCTC AGCTACAAGG GGGGCAACAC GCGCACAGGG 
GCCGCGATTC TCCACGTGGC TGACCGCGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GTATCCTCAT CACAGACGGG AAGTCCCAGG ACATGGTGGA CACAGCTGCC 
CAGAGGTTGA AGGGGCAGGG AGTCAAGCTG TTTGCTGTGG GCATCAAGAA TGCCGACCCC 
GAGGAGCTGA AGCGAATTGC CTCACAGCCG ACTAGCGATT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACGCT CCTGCCTCTG GTTTCCCGGA GGGTGTGCAC AACTGCGGGT 
GGTGTGCCCG TGACCCTGCC CCCCGACGAC TCGACCTCTG GTCCACGAGA CCTGCTGCTT 
GAGCCAGGCA GTCAGTCCTT GAGAGTCCAG TGGACAGCGG CCAGTGGCCC CGTGACTGGC 
TACAAGGTCC AGTACGCACC CCTGACAGGG CTGGGACAGC CATTGCCAAG CGAGCTGCGG 
GAGGTGAGCG TCCCAGCTGG TGAGACCAGC GTGCAGCTGC AGGGTCTCCG GCCACTGACT 
GAGTACCAAG TGACGGTGGT CGCCCTCTAC GCCAACAGCA TCGGGGAGGC CGTGAGCGGG 
ACCGCTAGGA CCACTGCTCT GGAGGGGCCA GAAGTGACCA TCCAGAACAC CACAGCCCAC 
AGCCTCCTGG TGGCCTGGCA GAGTGTGCCA GGTGCCACTG GCTACCGGGT GACGTGGCGG 
GTCGTCAGGG GTGGGGCCAC ACAGCAGCAG GAGCTGGGCT CTGGGCAGGG GTCAGTGTTG 
CTGAGGGACC TGCAGCCTGG CACCGACTAC GAGGTGACCG TGAGTGCCCT GCTTGGCCGG 
AGCGTGGGGC CAGCCACCTC CCTGACTGCC CGCACTGACA CGTCCGTCGA GCAGACCCTG 
CGTCCTATCA TCCTGGGCCC CACGTCCATC CTCCTCTCCT GGAACCTGGT GCCTGAGGCC 
CGTGGCTACC GGCTGGAATG GCGGCGTGAG AGCGGCTTAG AGGTGCCACA GAAGGTGGTG 
CTGCCCTCTG ACGTGACCCG CTACCAGTTG GATGGGCTGC AGCCAGGCAC CGAGTACCGC 
CTCACTCTCT ACACGCTGCT AGAGGGCCGT GAGGTGGCCA CCCCAGCGAC CGTGGTCCCC 
ACTGAGCTGC CCGTGGGCCC CGTCACAGCC CTCCAGGCCA CCGAGCTGCC TGGGCAGCGT 
GTGCGAGTGT CCTGGAGCCC AGTTCCCGGC GCCACCGAGT ATCGCATCAC TGTGCGCAGC 
GCCCAGGGGG TTGAGCGGAG CCTGGTGCTT CCTGGGAGTC AGACGGCCTT TGACTTGGAT 
GACGTCCGGG CTGGGCTCAG CTACACAGTG CGGGTGTCAG CGCGAGTAGG CGGCCGGGAG 
GGCGCTGCCA GCATCCTCAC TGTCCGCCGG GAGCCAGAAC CCCCACTGGC CATCCCAGGG 
CTGCGGGTCA TGGCATCGGA CGCAACGCGA GTGAGGGTGG CCTGGGGACC AGTTCCCGGA 
GCCAGCGGAT TTCGGATTAG CTGGAGGACA GACAATGGTC TGGAGTCCAG CCAGACAGTT 
CCTTCAGACT CTACTGCCAC CGACATTGTG GGGCTGAGAC CTGGGACCTC CTACCAGGTG 
GCCGTGTCGG CGCTGCGAGG AAGAGAGGAG GGCCCTCCTG CGGTCATCGT GGCTCGAACC 
GATCCCTTGG GCCCAGTGAG ATCGGTCCAT GTGACTCGAG TCAGCAGCTC ATCTGTCACC 
ATCACCTGGA ACCGGGTTCC TGGTGCCACA GGGTACAGGG TCTCCTGGCG CTCAGGCCGA 
GGCCCAGAGA AATCCCAGTT GGTTTCTGGG GAGGCCACGG TGGCCGAGCT GGATGGACTG 
GAGCCAGATA CTGAGTACAC CGTGCGTGTG TGGGCCCGTG TGGCTGGTGT GGACGGGACG 
CCTGCCTCTG CGGTTGTGAG AACTGACCCT CGGCCCGTGG GCAGTGTGTC GAAGCTGCAG 
ATCCATAATG CTTCCAGTGA CGTTCTGCGG GTCACCTGGG TGGGGGTCCC TGGAGCCACA 
GCCTACAGAC TGGCCTGGGG CCGGAGTGAG GGTGGCCCCA CAAGACAGCA GATTCTCCCA 
GGAAACACGG ACTCCGCAGA AATACGGGGC CTCGAAGGCG GAGTCAGTTA TTCAGTGAGA 
GTGACCGCAC TCATAGGGGA CCGCGAGGGC GCGCCTGTCT CCATTGTCGT CACCACCCCT 
CCGGAGGCTC CTCCAGCCCT GGAGACACTT CGCGTGGAGC AGCGAGGGGA GCACTCACTG 
AGGCTGCACT GGCAGCCGGT GCCTGGTGCA CGCGGCTTCC GTCTGCGTTG GCGACCTGAG 
GGTGGCCAGG AACAGTCCCG AGTCCTGGGG CCGGAAGTCA GCAGCTATGA CCTGGATGGG 
CTGGAGCCAG CGACCCACTA CCGCATATGG CTGAGCGTCT TGGGGACGGC TGGAGAAGGG 
CCCCCCTCGG AAGTGACTGC ATACACTGAG TCACCTCGTG TCCCAAGCAC TCAACTGCGT 
GTGGTGGACA CGTCAGTCGA CTCAGTGACT CTGGCCTGGA CCCCAGTGTC TGGGGTATCC 
AGCTACATCC TATCCTGGCG GCCTCTCAGA GGCCCTGGCC AAGAACTGCC TGGGGCCTCA 
CAGACACTTC CGGGGACCTC AAGTTCCCAG CGGGTGACAG GCCTAGAGCT GGGCATCTCC 
TACCTCTTCT CCCTAACTCC TGTCCGGGAC GGTGTACGCG GTCCTGAAGC CTCTATCACA 
CAGAGCCCAG CGTGTCCCCG CGGCCTGATG GATGTGGTGT TCCTGGTGCA CGCCACTCGA 
GACAATGCTC ATCGCTCGGA GGCTGTGAAG CGAGCCCTGG AGCACCTGGT GTCTGCACTC 
GGGCCTCTCG GGCCACAGGC CGTCCAGGTC GGCCTCCTGT CCTACAGTCA CCGGCCCTCC 
CCACTGTTGT CGCTGAACAG CTCCCATGAC CTTGGCGTCA TCCTGCAGAA GATCCGCAAC 
ATCCCCTACA CGGACCCAAG TGGGAACAAC CTGGGCACAG CCGTGGTTAC AGCCCACAGA 
TACCTGCTGG CACCAGATGC GCCTGGACGC CGCCGGCATG TGCCGGGGGT GATGGTTCTC 
CTGGTGGATG AGCCCTTGAG AGGTGACATC TTCAACTCTA TCCGTGAGGC CCAGGCTGCT 
GGGCTCAGAG TGATGATGCT GGGGCTGGCG GGAGCTGACC CAGAGCAGCT GCGTCGCTTG 
GTGCCTGGCA TGGACCCTTC CCAGACCTTC TTCGCTGTGG ATGATGGTCT GAGCTTGGAG 
CGGGCCGTCA GTAGTCTGGC AGCAGCCCTG TGTCAGACAG CCTTGACCAC GCAGCCACAG 
CCAGAGCCTT GCTCAGTGCA TTGTCCAAAG GGCCAGAAGG GGGAACCCGG ACAGATGGGC 
CTGAAAGGAC AAGCCGGGCC TCCTGGCCCC CCCGGCCTCC CGGGCAGGAT TGGTGCTCCT 
GGCCCCCAGG GACCTCCTGG AAGTGCTTCT GCGAAGGGCG AGAGGGGCTT CCCTGGGGCA 
GATGGGCCTC CAGGCAGCCC TGGCCGCCCT GGGACTCCTG GAGCCCCTGG TCCGAAGGGC 
TCCCCAGGGT GGGCCGGCCC TCGTGGAGAA CCAGGAGAGC GAGGACCTCG AGGCCCAAAG 
GGGGAGCCGG GAGAGCCTGG ACAAGTCATT GGAGGCGAGG GCCCAGGGCT TCCTGGGCAG 
AAAGGGGACC CTGGACCTCC AGGTCCCCCT GGATCTCGTG GGCCGTTGGG GGACCCGGGA 
CCCCGGGGTC CCCCGGGATT TCCTGGAACA GCTGTGAAGG GTGAGAAAGG CGATCGTGGG 
GAGCAGGGCC CCCCTGGACC AGGTGACGGC ACTGCTGCTC TGGGCGAGCC TGGGCTGCCG 
GGTCTTCCTG GAAGCCCCGG ACCCCAAGGC TCAGTTGGCC CCCCTGGAGA GAAAGGAGAA 
AAGGGTGACT GTGAAGATGG AGCCCCGGGC CTCCCAGGAC AACCCGGAAT TCCGGGTGAG 
CGGGGCCTTC GGGGACTTCC TGGAGATGCT GGCCCCAAAG GCGACCGAGG ACTAACAGGG 
GCCGCTGGAG AGAAGGGTGA ACGTGGATCG CCTGGCCCAG TGGGACCACA GGGGCTGCCA 
GGAGTTGCTG GACGACCTGG GCCCGAGGGT CCTGAAGGGC CACCAGGACC GACCGGCCGC 
CGAGGAGAGA AGGGGGAGCC TGGTCGGCCT GGAGACCCTG CAGTGGGACC TGGGGGTGCT 
GGAGCTAAAG GAGAGAAGGG AGATATGGGC CCCCCCGGGC CCAAAGGAGC TACCGGATTC 
AAAGGGGAAC AGGGCTCACC TGGCTTGGCT CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC 
CCTGGAGGCC GAGGTCCCAT TGGCCTCACT GGCAGAGCAG GACCCCCGGG CGACTCAGGA 
CCCCCTGGAG AGAAGGGAGA CCCTGGGCGG CCCGGTTCCC CAGGACCCGT CGGCCCCCGA 
GGACGAGACG GTGAAGTTGG AGAGAAGGGC GATGACGGTC CCCCGGGTGA CCCAGGTTTG 
CCTGGAAAAG CTGGCGAGCG TGGCCTTCGG GGAGCGCCTG GAGCTCGGGG GCCTGTGGGT 
GAGAAGGGAG ACCAGGGAGA TCCTGGAGAG GAAGGACGAA ATGGGAGCCC TGGACCGTCT 
GGACCCAAGG GTGACCGTGG GGAGCCAGGT CCCCCAGGAG CCCCTGGACG GCTGGTGGAC 
GTGGGACTTG GAGCCGGAGA GAAGGGAGAG CCTGGGGACC GAGGACAGGA GGGTCCACGA 
GGACCCAAGG GCGACCCTGG CCCCCCTGGA GCTTCTGGGG AGAGGGGCGT CAGTGGACTT 
CGGGGGCCTC CAGGCCCACA GGGGGACCCA GGTGTTCGAG GCCCAGCAGG AGAAAAGGGC 
GACCGGGGCC CTCCTGGCCT GGATGGCCGC AGTGGGCTGG ATGGGAAACC GGGAGCCTCT 
GGTCCCCCTG GGCCGAATGG TGCCATGGGC AAGGCCGGAG ACCCAGGGAG AGACGGACTT 
CCGGGCCTTC GAGGAGAACA GGGCCCCCCT GGCCCTGCTG GTCCCCCTGG AGCACCGGGA 
AAGCCGGGCG AGGATGGCAA GCCTGGCCTG AATGGGAAAA ACGGAGAACC CGGGGACCCT 
GGAGAAGACG GGAGGAAGGG AGAGAAGGGA GATTCAGGCG CCCCTGGGAG AGAAGGTCGT 
GACGGCCCCA AGGGTGAGCG TGGAGCTCCT GGTAGCCCTG GACTCCAGGG CCCCCCGGGC 
CTCCCAGGGC AGGTCGGCCC TCCCGGGCAG GGCTTCCCTG GCGTCCCGGG GAGTTCAGGC 
CCCAAGGGTG ACCGTGGGGA GACTGGACCC AGAGGGGAGC AGGGCCTCCC AGGAGAGCGT 
GGCCTGAGAG GAGAGCCTGG GAGCCTGGTG AATGCAGAGC GGTTGCTGGA AAATATTGGC 
ATCAAGACAT CTGCCCTGAG GGAGATCGTG GAGACCTGGG AAGAAAGCTC TGGTGGCTTC 
CTGCCCACGC CTGAACGGCG TCGCGGCCCC AAGGGGGACC CAGGCGAGCG GGGCCCCCCA 
GGCAAGGAGG GCCCCATCGG CTTTTCTGGA GAACGTGGAC CAAAGGGAGA TCGTGGAGAC 
CCCGGCCCTC AGGGGCCACC TGGCCTGGCC CTGGGGGAGA GGGGCCTTCC TGGACCTACC 
GGCCTTGCCG GGGAGCCTGG GAAGCCTGGT ATCCCTGGGC TCCCTGGCCC AGCTGGGAGT 
GTGGGGGAGG CCGGAAGACC CGGAGAGAGG GGAGAACGGG GAGAGAAAGG AGAACGTGGA 
GAACAGGGCA GAGACGGCCT TCCTGGCCTC CCTGGACCCC CCGGACCCCC AGGCCCCAAG 
GTGGCCGTGG ACGAGCCAGG TTCTGGGCTC TCTAGAGAAC AAGGACCCCC TGGATTCAAG 
GGTGCTAAGG GGGATCCAGG CAGTGATGGC GACCGAGGCC CCAAAGGAGA CAGGGGTGAG 
CCGGGCATCA AGGGCGACCG GGGAGAGCCT GGACAGAGGG GTCCGAATGG CAGCCCGGGT 
CTGCCAGGAG AGCGCGGCGT GGCTGGGCCC GAGGGGAAGC CGGGTCTGCA AGGTCCACGG 
GGGACCCCTG GCCCGGCGGG CGGCCATGGA GACCCTGGAC CACCCGGGGC CCCGGGTCTT 
GCTGGCCCTG CGGGACCCCA GGGACCTTCT GGCCTAAAGG GGGAGCCCGG AGAGACAGGA 
CCACCAGGAC GGGGCCTGCC TGGACCTACT GGAGCCGTGG GACTTCCCGG GCCCCCTGGC 
CCTTCAGGCC TGGTGGGTCC TCAAGGGGCA CCGGGTTTGC CTGGACAAGT GGGGGAGACG 
GGAAAGCCGG GAGCGCCAGG TCGTGATGGT GCCACTGGGA AAGATGGAGA CAGAGGAGCC 
CCCGGTGTGC CGGGGTCACC AGGTCTGCCA GGCCCTGTCG GACCTAAAGG AGAGCCGGGA 
CCCATGGGGA CCCCTGGACA GGCTGTGGTC GGGCCCCCTG GAGCAAAGGG AGAGAAGGGC 
GCCCCTGGAG CCCTTGCTGG AGACCTGGTG GGAGAGCCGG GAGCCAAGGG TGACCGAGGA 
CTGCCAGGGC CGCGGGGCGA GAAGGGTGAA GCTGGCCGTG CGGGGGAGCC TGGAGACCCT 
GGCGAAGATG GTCAGAAGGG GGCTCCGGGA CCCAAAGGTC ACAAGGGTGA CCCAGGAGTT 
GGGGTCCAGG GGCCCCCTGG GCCAGTTGGC CCTCCAGGTC TAAAGGGAGA CTCAGGGCCC 
CCCGGATCCC CCGGACCTCC TGGTATTGTG GGGTTCCCCG GTCAGACAGG CCCTCGAGGA 
GAGCTGGGGC AGCCGGGCCC CAGTGGAGAG CGGGGTCTGG CAGGTGCCCC AGGGAGAGAG 
GGAGCCCCAG GTCCCTTGGG GCCGCCTGGA CCACCTGGGT CAGCGGGAGT ACCTGGGGTC 
CCTGGACTCA AAGGAGACAA GGGTGACACT GGAGCAGGGC TGCCTGGGGC CCGAGGCGAG 
CGTGGGGAGC CCGGTGTCCG GGGTGAAGAT GGCCGCCCCG GCCTGGAGGG ACCCCGAGGA 
CTTGCGGGTC CCCCAGGCAT CCGGGGAGAA CGTGGGGAGA AGGGTGACGC TGGTGCCTCA 
GGACTGAAGG GTGACAAGGG CGACTCAGCT GTGATTTTGG GGCCTCCGGG GCCACGGGGT 
GCCAAGGGGG ACTCGGGTGA ACGAGGGCCT CGGGGAATAG ATGGTGACAA AGGACCTCGG 
GGAGACAGCG GGGAACCTGG AGAGAAGGGC ACCAAGGGAG AGCCTGGTGA CAAGGGCTCA 
GCCGGGCTGC TGGGAGCGCG CGGACTCATG GGACCCAAGG GCGAGCCTGG TGCCGCAGGG 
ATCCCCGGCG AACCGGGATC CCCAGGAAAA GACGGAGTCC CTGGTGTCCG AGGAGACAAA 
GGAGATGTTG GCTTCATGGG TCCCCGGGGC CTCAAGGGTG AACGGGGAAT TAAGGGAGCC 
TGTGGCCTCG ACGGAGAGAA GGGAGATAAG GGAGAAGCCG GCCCCCCGGG CCGCCCAGGG 
CTGGCAGGAC GCAGAGGAGA GCTGGGGGAG CCGGGTGTTC CAGGCCAGGC AGGGGCCCCT 
GGGAAGGAGG GCCTGATCGG TCCCAAGGGT GACCGAGGTT TCGATGGGCA GCCAGGACCC 
AAGGGTGACC AGGGCGAGAA AGGGGAGCGG GGACCCCCAG GAGTGGGGGG CTTCCCAGGT 
CCCAGGGGCA GTGATGGCTC TAGTGGTCCT CCCGGGCCAC CTGGCAGCAT TGGTCCCAAA 
GGCCCTGAAG GACTTCAGGG TCAGAAGGGT GAGCGAGGTC CCCCTGGAGA GAGCGTGGTG 
GGTGCCCCTG GGGCCCCTGG AACTCCTGGA GAGAGAGGGG AGCAGGGGCG ACCAGGCCCC 
GCGGGGCCCC GTGGTGAAAA GGGAGAAGCT GCGCTGACGG AGGATGACAT TCGGGGCCTT 
GTGCGTCAGG AGATGAGTCA ACATTGCGCC TGTCAGGGCC AGTATATCGC ATCTGGATCA 
CGGCCTCTCC CGAGTTACGC AGCAGACACC GCTGGCCCGC AGCTCCACCC CGTGCCCGTG 
CTCCGCGTGT CCCACGCAGA GGAGGACGGC CAGGTGCCCC CCGAGGACGA TGAGTATGAA 
TACTCTGAGT ATTCCATGGA GGAATACCAG GACCCCGCGG CTCCTTGGGA TGACGTTGAC 
CCCTGCTCGC TTCCACTGGA CGAGGGTTCC TGCAGTGCCT ACACCCTGCG CTGGTATCAT 
CGGGCTGGGG CGGGGGGCAC GAAGGCCTGT CACCCCTTCG TCTACGGTGG CTGCGGAGGG 
AATGCCAACC GTTTTGGGAC CCGGGAGGCC TGTGAGCACC GCTGCCTGCC CCAGGCGGCC 
CACAGTCAAG GGACAGGTGC TGCCCGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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COL7A1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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