COL2A1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL2A1
Entrez Gene ID	407142
Full Name	collagen type II alpha 1 chain
Synonyms	CB11
General protein information	Preferred Names collagen type II alpha 1 chain Names collagen alpha-1(II) chain alpha-1 type II collagen collagen, type II, alpha 1 pro-alpha 1(II)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113224.2	NP_001106695.1	collagen alpha-1(II) chain isoform 2 precursor
NM_001001135.3	NP_001001135.2	collagen alpha-1(II) chain isoform 1 preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	COL2A1	NP_001001135.2
Gallus gallus (chicken)	COL2A1	NP_989757.1
Xenopus tropicalis (tropical clawed frog)	col2a1	NP_989220.1
Macaca mulatta (Rhesus monkey)	COL2A1	XP_001100739.1
Canis lupus familiaris (dog)	COL2A1	NP_001006952.1
Rattus norvegicus (Norway rat)	Col2a1	NP_037061.1
Danio rerio (zebrafish)	col2a1a	NP_571367.1
Pan troglodytes (chimpanzee)	COL2A1	XP_509026.2
Homo sapiens (human)	COL2A1	NP_001835.3
Mus musculus (house mouse)	Col2a1	NP_112440.2

Gene Ontology
Bibliography

Related articles in PubMed

Lethal chondrodysplasia in a family of Holstein cattle is associated with a de novo splice site variant of COL2A1.
Agerholm JS, Menzi F, McEvoy FJ, Jagannathan V, Drögemüller C
BMC veterinary research12100(2016 Jun)

Definition of the native and denatured type II collagen binding site for fibronectin using a recombinant collagen system.
An B, Abbonante V, Yigit S, Balduini A, Kaplan DL, Brodsky B
The Journal of biological chemistry289(8)4941-51(2014 Feb)

Two subsystems of meniscal collagen and their different thermal stabilities.
Ignatieva NY, Zakharkina OL, Lunin VV, Baratova LA, Grokhovskaya TE, Balalaeva IV, Sergeeva EA
Doklady. Biochemistry and biophysics444175-7(2012 May-Jun)

Disc strain and resulting positive mRNA expression from application of a noninvasive treatment.
Desmoulin GT, Hewitt CR, Hunter CJ
Spine36(14)E921-8(2011 Jun)

Free axial vibrations at 0 to 200 Hz positively affect extracellular matrix messenger ribonucleic acid expression in bovine nucleus pulposi.
Desmoulin GT, Reno CR, Hunter CJ
Spine35(15)1437-44(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following COL2A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL2A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa134046	NM_001113224.2 Latest version!	Bos taurus collagen type II alpha 1 chain (COL2A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa134048	NM_001001135.3 Latest version!	Bos taurus collagen type II alpha 1 chain (COL2A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL2A1 ( NM_001113224.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001106695.1 Bos taurus collagen type II alpha 1 chain (COL2A1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OBa134046
Clone ID Related Accession (Same CDS sequence)	NM_001113224.2
Accession Version	NM_001113224.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	collagen alpha-1(II) chain isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02012985.1 and DAAA02012986.1. On Oct 1, 2015 this sequence version replaced NM_001113224.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in a shorter protein. The resulting protein (isoform 2) lacks the von Willebrand factor type C (VWC) domain, compared to isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignment of the human homolog (NM_033150.2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145430 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC 
CTTCGGTGTC ACGGCCAGGA TGTCCGGCAA CCAGGACCAA AGGGACAGAA AGGAGAACCT 
GGAGATATCA AGGACATCGT AGGACCCAAG GGACCTCCTG GACCTCAGGG ACCCGCAGGT 
GAACAAGGAC CCAGAGGTGA TCGTGGTGAC AAAGGTGAAA AAGGTGCTCC TGGACCTCGT 
GGCAGAGATG GAGAGCCTGG GACCCCTGGA AACCCTGGCC CCCCTGGTCC TCCCGGCCCC 
CCTGGCCCCC CTGGCCTTGG TGGAAACTTT GCTGCCCAGA TGGCTGGTGG ATTTGATGAG 
AAGGCTGGTG GCGCCCAGAT GGGAGTAATG CAGGGACCAA TGGGCCCCAT GGGACCTCGG 
GGACCCCCAG GCCCTGCTGG TGCTCCCGGA CCTCAAGGAT TTCAAGGCAA CCCTGGTGAA 
CCTGGTGAAC CCGGTGTTTC TGGTCCCATG GGCCCCCGTG GTCCTCCTGG CCCCCCTGGG 
AAACCTGGTG ATGATGGTGA AGCTGGAAAG CCTGGAAAAT CTGGCGAGAG AGGCCCTCCT 
GGCCCTCAGG GTGCTCGTGG TTTCCCAGGA ACCCCAGGCC TTCCTGGTGT CAAAGGTCAC 
AGAGGTTACC CAGGTCTAGA TGGTGCTAAG GGAGAAGCTG GTGCTCCAGG TGTGAAGGGT 
GAGAGTGGTT CCCCAGGTGA GAACGGTTCT CCAGGCCCAA TGGGTCCCCG CGGCTTGCCT 
GGTGAGAGAG GACGGACTGG CCCTGCCGGC GCTGCAGGTG CTCGAGGCAA TGACGGTCAG 
CCAGGCCCTG CAGGGCCTCC GGGTCCTGTG GGTCCTGCTG GCGGTCCTGG CTTTCCTGGT 
GCTCCTGGTG CCAAGGGTGA AGCTGGCCCC ACCGGTGCTC GAGGTCCCGA AGGCGCCCAG 
GGTCCTCGCG GTGAACCGGG TACTCCTGGG TCCCCTGGGC CAGCTGGTGC TGCTGGCAAC 
CCTGGAACTG ATGGAATCCC TGGAGCCAAG GGATCTGCTG GTGCCCCTGG CATTGCTGGT 
GCTCCCGGCT TCCCTGGACC CCGTGGTCCA CCCGGCCCTC AAGGTGCAAC TGGTCCTCTG 
GGCCCAAAAG GTCAAACGGG TGAGCCTGGT ATTGCTGGCT TCAAAGGCGA ACAAGGCCCC 
AAGGGAGAAC CGGGCCCTGC TGGTCCCCAA GGAGCCCCTG GTCCTGCTGG TGAAGAAGGG 
AAAAGAGGTG CCCGTGGAGA ACCTGGTGGT GCTGGGCCCG CCGGTCCCCC TGGAGAAAGA 
GGCGCTCCTG GAAACCGTGG TTTCCCAGGT CAGGATGGTC TGGCAGGTCC CAAGGGAGCC 
CCTGGAGAGC GAGGACCCAG TGGCCTCGCT GGTCCCAAAG GCGCCAACGG TGACCCTGGC 
CGTCCCGGAG AGCCTGGCCT TCCTGGAGCC CGGGGTCTCA CTGGTCGCCC TGGTGATGCT 
GGTCCTCAAG GCAAAGTTGG TCCTTCCGGA GCCCCTGGTG AAGACGGTCG CCCTGGACCC 
CCAGGTCCTC AGGGGGCTCG TGGGCAGCCT GGTGTCATGG GTTTCCCTGG CCCCAAAGGT 
GCCAATGGTG AGCCTGGCAA AGCTGGTGAG AAAGGACTGC CTGGTGCTCC TGGTCTGAGA 
GGTCTTCCTG GCAAAGATGG TGAGACAGGT GCTGCAGGCC CCCCTGGACC CGCTGGACCA 
GCTGGTGAAC GAGGCGAGCA GGGTGCTCCT GGGCCATCTG GGTTCCAGGG ACTTCCTGGC 
CCTCCCGGCC CCCCAGGTGA AGGTGGAAAA CCAGGTGACC AGGGTGTTCC TGGTGAAGCT 
GGAGCTCCCG GCCTCGTGGG TCCCAGGGGT GAACGAGGTT TCCCAGGAGA ACGTGGCTCC 
CCCGGTTCCC AGGGCCTCCA GGGGGCCCGC GGTCTCCCCG GCACTCCTGG CACTGATGGT 
CCCAAAGGCG CAGCTGGCCC AGCTGGCCCC CCTGGGGCTC AGGGCCCTCC AGGTCTGCAG 
GGCATGCCCG GTGAGAGGGG AGCAGCTGGT ATCGCCGGAC CCAAGGGAGA CAGGGGTGAT 
GTTGGTGAGA AAGGCCCCGA GGGCGCCCCC GGGAAGGACG GTGGACGAGG TCTGACTGGC 
CCCATTGGCC CCCCTGGCCC AGCCGGAGCC AACGGTGAGA AGGGAGAAGT TGGACCCCCT 
GGTCCTGCTG GAACTGCTGG TGCTCGAGGT GCCCCGGGTG AACGTGGAGA GACAGGCCCC 
CCTGGACCTG CTGGATTCGC CGGTCCCCCT GGTGCTGATG GCCAGCCTGG TGCCAAAGGT 
GAACAAGGAG AAGCCGGCCA GAAAGGTGAC GCTGGTGCCC CCGGTCCTCA GGGCCCCTCT 
GGAGCTCCTG GGCCTCAGGG TCCTACTGGT GTGACCGGTC CTAAAGGAGC CCGAGGTGCT 
CAAGGGCCCC CGGGAGCCAC CGGATTCCCC GGAGCAGCTG GCCGCGTCGG ACCCCCAGGC 
TCCAATGGCA ACCCTGGACC CCCTGGTCCT CCTGGTCCTT CTGGAAAAGA TGGTCCTAAA 
GGTGCTCGAG GCGACAGTGG GCCCCCTGGC CGAGCTGGTG ACCCTGGCCT CCAAGGTCCT 
GCTGGACCCC CTGGCGAGAA GGGAGAGCCT GGAGATGATG GTCCTTCTGG TCCCGATGGT 
CCTCCAGGTC CTCAGGGTCT GGCTGGTCAG AGGGGCATCG TTGGTCTGCC TGGACAGCGT 
GGTGAGCGAG GATTCCCCGG CCTCCCCGGC CCCTCGGGTG AGCCTGGCAA GCAGGGAGCC 
CCTGGAGCAT CTGGAGACCG AGGTCCCCCT GGCCCTGTGG GTCCTCCTGG CCTGACTGGT 
CCTGCTGGTG AGCCTGGACG TGAGGGAAGC CCCGGTGCTG ACGGCCCCCC TGGCAGAGAT 
GGCGCAGCTG GAGTCAAAGG TGACCGTGGA GAGACCGGTG CCGTGGGTGC TCCTGGAGCC 
CCTGGGCCCC CTGGCTCTCC TGGCCCCGCT GGCCCAATTG GCAAGCAGGG AGACCGAGGA 
GAAGCTGGCG CACAAGGTCC CATGGGACCT GCAGGACCTG CTGGAGCCCG GGGAATGCCA 
GGCCCTCAAG GCCCCCGAGG TGACAAAGGA GAAACTGGAG AGGCTGGCGA GAGGGGACTG 
AAGGGACACC GTGGCTTCAC TGGTCTGCAG GGTCTGCCCG GCCCCCCTGG TCCTTCTGGA 
GACCAGGGTG CTTCCGGTCC TGCAGGTCCT TCCGGCCCCA GAGGTCCTCC TGGTCCCGTC 
GGTCCCTCTG GCAAAGATGG TGCTAACGGA ATCCCTGGCC CCATTGGACC TCCTGGGCCC 
CGTGGACGTT CCGGCGAGAC TGGCCCTGCT GGTCCTCCTG GAAACCCTGG ACCCCCTGGC 
CCTCCTGGCC CCCCCGGCCC TGGCATCGAC ATGTCCGCCT TTGCTGGCCT TGGCCAGAGA 
GAGAAAGGCC CCGATCCTCT GCAGTACATG CGGGCCGACG AGGCAGCCGG CAACCTGAGA 
CAGCATGATG CCGAGGTGGA CGCCACACTC AAGTCCCTCA ACAACCAGAT CGAGAGCCTC 
CGTAGCCCCG AGGGCTCCCG CAAGAACCCA GCTCGCACCT GCCGAGACCT GAAACTCTGC 
CACCCTGAGT GGAAGAGCGG AGACTACTGG ATCGACCCCA ACCAGGGCTG CACCCTGGAT 
GCCATGAAGG TTTTCTGCAA CATGGAGACT GGCGAGACCT GCGTCTACCC CAACCCGGCC 
AGCGTCCCCA AGAAGAACTG GTGGAGCAGC AAGAGCAAGG ACAAGAAACA CATCTGGTTT 
GGAGAAACCA TCAACGGTGG CTTCCACTTC AGCTATGGAG ATGACAACCT GGCTCCCAAC 
ACCGCCAACG TCCAGATGAC CTTCCTGCGC CTGCTGTCCA CCGAGGGCTC TCAGAACATC 
ACCTACCACT GCAAGAACAG CATTGCCTAC CTGGACGAAG CTGCTGGCAA CCTCAAGAAG 
GCTCTGCTCA TCCAGGGCTC CAACGACGTG GAGATCCGGG CTGAGGGCAA CAGCAGGTTC 
ACATATACCG TTCTGAAGGA TGGCTGCACG AAACACACCG GTAAGTGGGG CAAGACTATG 
ATCGAGTACC GGTCACAGAA GACCTCCCGT CTGCCCATCA TTGACATTGC ACCCATGGAC 
ATAGGAGGGC CCGAGCAGGA ATTCGGTGTG GACATAGGGC CTGTCTGCTT CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106695.1
CDS	27..4283
Translation	MIRLGAPQTLVLLTLLVAAVLRCHGQDVRQPGPKGQKGEPGDIKDIVGPKGPPGPQGPAGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGPPGPPGPPGPPGLGGNFAAQMAGGFDEKAGGAQMGVMQGPMGPMGPRGPPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPPGKPGDDGEAGKPGKSGERGPPGPQGARGFPGTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDGQPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGPRGEPGTPGSPGPAGAAGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEPGIAGFKGEQGPKGEPGPAGPQGAPGPAGEEGKRGARGEPGGAGPAGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLAGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLPGAPGLRGLPGKDGETGAAGPPGPAGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKPGDQGVPGEAGAPGLVGPRGERGFPGERGSPGSQGLQGARGLPGTPGTDGPKGAAGPAGPPGAQGPPGLQGMPGERGAAGIAGPKGDRGDVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPAGTAGARGAPGERGETGPPGPAGFAGPPGADGQPGAKGEQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGSNGNPGPPGPPGPSGKDGPKGARGDSGPPGRAGDPGLQGPAGPPGEKGEPGDDGPSGPDGPPGPQGLAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGRDGAAGVKGDRGETGAVGAPGAPGPPGSPGPAGPIGKQGDRGEAGAQGPMGPAGPAGARGMPGPQGPRGDKGETGEAGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGANGIPGPIGPPGPRGRSGETGPAGPPGNPGPPGPPGPPGPGIDMSAFAGLGQREKGPDPLQYMRADEAAGNLRQHDAEVDATLKSLNNQIESLRSPEGSRKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPASVPKKNWWSSKSKDKKHIWFGETINGGFHFSYGDDNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEIRAEGNSRFTYTVLKDGCTKHTGKWGKTMIEYRSQKTSRLPIIDIAPMDIGGPEQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	NM_001113224.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type II alpha 1 chain (COL2A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113224.2

ORF Insert Sequence:

ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC 
CTTCGGTGTC ACGGCCAGGA TGTCCGGCAA CCAGGACCAA AGGGACAGAA AGGAGAACCT 
GGAGATATCA AGGACATCGT AGGACCCAAG GGACCTCCTG GACCTCAGGG ACCCGCAGGT 
GAACAAGGAC CCAGAGGTGA TCGTGGTGAC AAAGGTGAAA AAGGTGCTCC TGGACCTCGT 
GGCAGAGATG GAGAGCCTGG GACCCCTGGA AACCCTGGCC CCCCTGGTCC TCCCGGCCCC 
CCTGGCCCCC CTGGCCTTGG TGGAAACTTT GCTGCCCAGA TGGCTGGTGG ATTTGATGAG 
AAGGCTGGTG GCGCCCAGAT GGGAGTAATG CAGGGACCAA TGGGCCCCAT GGGACCTCGG 
GGACCCCCAG GCCCTGCTGG TGCTCCCGGA CCTCAAGGAT TTCAAGGCAA CCCTGGTGAA 
CCTGGTGAAC CCGGTGTTTC TGGTCCCATG GGCCCCCGTG GTCCTCCTGG CCCCCCTGGG 
AAACCTGGTG ATGATGGTGA AGCTGGAAAG CCTGGAAAAT CTGGCGAGAG AGGCCCTCCT 
GGCCCTCAGG GTGCTCGTGG TTTCCCAGGA ACCCCAGGCC TTCCTGGTGT CAAAGGTCAC 
AGAGGTTACC CAGGTCTAGA TGGTGCTAAG GGAGAAGCTG GTGCTCCAGG TGTGAAGGGT 
GAGAGTGGTT CCCCAGGTGA GAACGGTTCT CCAGGCCCAA TGGGTCCCCG CGGCTTGCCT 
GGTGAGAGAG GACGGACTGG CCCTGCCGGC GCTGCAGGTG CTCGAGGCAA TGACGGTCAG 
CCAGGCCCTG CAGGGCCTCC GGGTCCTGTG GGTCCTGCTG GCGGTCCTGG CTTTCCTGGT 
GCTCCTGGTG CCAAGGGTGA AGCTGGCCCC ACCGGTGCTC GAGGTCCCGA AGGCGCCCAG 
GGTCCTCGCG GTGAACCGGG TACTCCTGGG TCCCCTGGGC CAGCTGGTGC TGCTGGCAAC 
CCTGGAACTG ATGGAATCCC TGGAGCCAAG GGATCTGCTG GTGCCCCTGG CATTGCTGGT 
GCTCCCGGCT TCCCTGGACC CCGTGGTCCA CCCGGCCCTC AAGGTGCAAC TGGTCCTCTG 
GGCCCAAAAG GTCAAACGGG TGAGCCTGGT ATTGCTGGCT TCAAAGGCGA ACAAGGCCCC 
AAGGGAGAAC CGGGCCCTGC TGGTCCCCAA GGAGCCCCTG GTCCTGCTGG TGAAGAAGGG 
AAAAGAGGTG CCCGTGGAGA ACCTGGTGGT GCTGGGCCCG CCGGTCCCCC TGGAGAAAGA 
GGCGCTCCTG GAAACCGTGG TTTCCCAGGT CAGGATGGTC TGGCAGGTCC CAAGGGAGCC 
CCTGGAGAGC GAGGACCCAG TGGCCTCGCT GGTCCCAAAG GCGCCAACGG TGACCCTGGC 
CGTCCCGGAG AGCCTGGCCT TCCTGGAGCC CGGGGTCTCA CTGGTCGCCC TGGTGATGCT 
GGTCCTCAAG GCAAAGTTGG TCCTTCCGGA GCCCCTGGTG AAGACGGTCG CCCTGGACCC 
CCAGGTCCTC AGGGGGCTCG TGGGCAGCCT GGTGTCATGG GTTTCCCTGG CCCCAAAGGT 
GCCAATGGTG AGCCTGGCAA AGCTGGTGAG AAAGGACTGC CTGGTGCTCC TGGTCTGAGA 
GGTCTTCCTG GCAAAGATGG TGAGACAGGT GCTGCAGGCC CCCCTGGACC CGCTGGACCA 
GCTGGTGAAC GAGGCGAGCA GGGTGCTCCT GGGCCATCTG GGTTCCAGGG ACTTCCTGGC 
CCTCCCGGCC CCCCAGGTGA AGGTGGAAAA CCAGGTGACC AGGGTGTTCC TGGTGAAGCT 
GGAGCTCCCG GCCTCGTGGG TCCCAGGGGT GAACGAGGTT TCCCAGGAGA ACGTGGCTCC 
CCCGGTTCCC AGGGCCTCCA GGGGGCCCGC GGTCTCCCCG GCACTCCTGG CACTGATGGT 
CCCAAAGGCG CAGCTGGCCC AGCTGGCCCC CCTGGGGCTC AGGGCCCTCC AGGTCTGCAG 
GGCATGCCCG GTGAGAGGGG AGCAGCTGGT ATCGCCGGAC CCAAGGGAGA CAGGGGTGAT 
GTTGGTGAGA AAGGCCCCGA GGGCGCCCCC GGGAAGGACG GTGGACGAGG TCTGACTGGC 
CCCATTGGCC CCCCTGGCCC AGCCGGAGCC AACGGTGAGA AGGGAGAAGT TGGACCCCCT 
GGTCCTGCTG GAACTGCTGG TGCTCGAGGT GCCCCGGGTG AACGTGGAGA GACAGGCCCC 
CCTGGACCTG CTGGATTCGC CGGTCCCCCT GGTGCTGATG GCCAGCCTGG TGCCAAAGGT 
GAACAAGGAG AAGCCGGCCA GAAAGGTGAC GCTGGTGCCC CCGGTCCTCA GGGCCCCTCT 
GGAGCTCCTG GGCCTCAGGG TCCTACTGGT GTGACCGGTC CTAAAGGAGC CCGAGGTGCT 
CAAGGGCCCC CGGGAGCCAC CGGATTCCCC GGAGCAGCTG GCCGCGTCGG ACCCCCAGGC 
TCCAATGGCA ACCCTGGACC CCCTGGTCCT CCTGGTCCTT CTGGAAAAGA TGGTCCTAAA 
GGTGCTCGAG GCGACAGTGG GCCCCCTGGC CGAGCTGGTG ACCCTGGCCT CCAAGGTCCT 
GCTGGACCCC CTGGCGAGAA GGGAGAGCCT GGAGATGATG GTCCTTCTGG TCCCGATGGT 
CCTCCAGGTC CTCAGGGTCT GGCTGGTCAG AGGGGCATCG TTGGTCTGCC TGGACAGCGT 
GGTGAGCGAG GATTCCCCGG CCTCCCCGGC CCCTCGGGTG AGCCTGGCAA GCAGGGAGCC 
CCTGGAGCAT CTGGAGACCG AGGTCCCCCT GGCCCTGTGG GTCCTCCTGG CCTGACTGGT 
CCTGCTGGTG AGCCTGGACG TGAGGGAAGC CCCGGTGCTG ACGGCCCCCC TGGCAGAGAT 
GGCGCAGCTG GAGTCAAAGG TGACCGTGGA GAGACCGGTG CCGTGGGTGC TCCTGGAGCC 
CCTGGGCCCC CTGGCTCTCC TGGCCCCGCT GGCCCAATTG GCAAGCAGGG AGACCGAGGA 
GAAGCTGGCG CACAAGGTCC CATGGGACCT GCAGGACCTG CTGGAGCCCG GGGAATGCCA 
GGCCCTCAAG GCCCCCGAGG TGACAAAGGA GAAACTGGAG AGGCTGGCGA GAGGGGACTG 
AAGGGACACC GTGGCTTCAC TGGTCTGCAG GGTCTGCCCG GCCCCCCTGG TCCTTCTGGA 
GACCAGGGTG CTTCCGGTCC TGCAGGTCCT TCCGGCCCCA GAGGTCCTCC TGGTCCCGTC 
GGTCCCTCTG GCAAAGATGG TGCTAACGGA ATCCCTGGCC CCATTGGACC TCCTGGGCCC 
CGTGGACGTT CCGGCGAGAC TGGCCCTGCT GGTCCTCCTG GAAACCCTGG ACCCCCTGGC 
CCTCCTGGCC CCCCCGGCCC TGGCATCGAC ATGTCCGCCT TTGCTGGCCT TGGCCAGAGA 
GAGAAAGGCC CCGATCCTCT GCAGTACATG CGGGCCGACG AGGCAGCCGG CAACCTGAGA 
CAGCATGATG CCGAGGTGGA CGCCACACTC AAGTCCCTCA ACAACCAGAT CGAGAGCCTC 
CGTAGCCCCG AGGGCTCCCG CAAGAACCCA GCTCGCACCT GCCGAGACCT GAAACTCTGC 
CACCCTGAGT GGAAGAGCGG AGACTACTGG ATCGACCCCA ACCAGGGCTG CACCCTGGAT 
GCCATGAAGG TTTTCTGCAA CATGGAGACT GGCGAGACCT GCGTCTACCC CAACCCGGCC 
AGCGTCCCCA AGAAGAACTG GTGGAGCAGC AAGAGCAAGG ACAAGAAACA CATCTGGTTT 
GGAGAAACCA TCAACGGTGG CTTCCACTTC AGCTATGGAG ATGACAACCT GGCTCCCAAC 
ACCGCCAACG TCCAGATGAC CTTCCTGCGC CTGCTGTCCA CCGAGGGCTC TCAGAACATC 
ACCTACCACT GCAAGAACAG CATTGCCTAC CTGGACGAAG CTGCTGGCAA CCTCAAGAAG 
GCTCTGCTCA TCCAGGGCTC CAACGACGTG GAGATCCGGG CTGAGGGCAA CAGCAGGTTC 
ACATATACCG TTCTGAAGGA TGGCTGCACG AAACACACCG GTAAGTGGGG CAAGACTATG 
ATCGAGTACC GGTCACAGAA GACCTCCCGT CTGCCCATCA TTGACATTGC ACCCATGGAC 
ATAGGAGGGC CCGAGCAGGA ATTCGGTGTG GACATAGGGC CTGTCTGCTT CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL2A1 ( NM_001001135.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001001135.2 Bos taurus collagen type II alpha 1 chain (COL2A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa134048
Clone ID Related Accession (Same CDS sequence)	NM_001001135.3
Accession Version	NM_001001135.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	collagen alpha-1(II) chain isoform 1 preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02012985.1 and DAAA02012986.1. On Oct 1, 2015 this sequence version replaced NM_001001135.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignment of the human homolog (NM_001844.4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145425 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC 
CTTCGGTGTC ACGGCCAGGA TGTCCAGAAG GCTGGCAGCT GTGTGCAGGA CGGGCAGAGG 
TATAATGATA AGGATGTGTG GAAGCCCGAG CCCTGCCGGA TCTGTGTCTG TGACACTGGG 
ACTGTCCTCT GCGACGACAT AATCTGTGAA GACATGAAAG ACTGCCTCAG CCCCGAGACC 
CCCTTCGGAG AGTGCTGCCC CATCTGCTCA GCTGACCTCC CCACTGCCAG TGGGCAACCA 
GGACCAAAGG GACAGAAAGG AGAACCTGGA GATATCAAGG ACATCGTAGG ACCCAAGGGA 
CCTCCTGGAC CTCAGGGACC CGCAGGTGAA CAAGGACCCA GAGGTGATCG TGGTGACAAA 
GGTGAAAAAG GTGCTCCTGG ACCTCGTGGC AGAGATGGAG AGCCTGGGAC CCCTGGAAAC 
CCTGGCCCCC CTGGTCCTCC CGGCCCCCCT GGCCCCCCTG GCCTTGGTGG AAACTTTGCT 
GCCCAGATGG CTGGTGGATT TGATGAGAAG GCTGGTGGCG CCCAGATGGG AGTAATGCAG 
GGACCAATGG GCCCCATGGG ACCTCGGGGA CCCCCAGGCC CTGCTGGTGC TCCCGGACCT 
CAAGGATTTC AAGGCAACCC TGGTGAACCT GGTGAACCCG GTGTTTCTGG TCCCATGGGC 
CCCCGTGGTC CTCCTGGCCC CCCTGGGAAA CCTGGTGATG ATGGTGAAGC TGGAAAGCCT 
GGAAAATCTG GCGAGAGAGG CCCTCCTGGC CCTCAGGGTG CTCGTGGTTT CCCAGGAACC 
CCAGGCCTTC CTGGTGTCAA AGGTCACAGA GGTTACCCAG GTCTAGATGG TGCTAAGGGA 
GAAGCTGGTG CTCCAGGTGT GAAGGGTGAG AGTGGTTCCC CAGGTGAGAA CGGTTCTCCA 
GGCCCAATGG GTCCCCGCGG CTTGCCTGGT GAGAGAGGAC GGACTGGCCC TGCCGGCGCT 
GCAGGTGCTC GAGGCAATGA CGGTCAGCCA GGCCCTGCAG GGCCTCCGGG TCCTGTGGGT 
CCTGCTGGCG GTCCTGGCTT TCCTGGTGCT CCTGGTGCCA AGGGTGAAGC TGGCCCCACC 
GGTGCTCGAG GTCCCGAAGG CGCCCAGGGT CCTCGCGGTG AACCGGGTAC TCCTGGGTCC 
CCTGGGCCAG CTGGTGCTGC TGGCAACCCT GGAACTGATG GAATCCCTGG AGCCAAGGGA 
TCTGCTGGTG CCCCTGGCAT TGCTGGTGCT CCCGGCTTCC CTGGACCCCG TGGTCCACCC 
GGCCCTCAAG GTGCAACTGG TCCTCTGGGC CCAAAAGGTC AAACGGGTGA GCCTGGTATT 
GCTGGCTTCA AAGGCGAACA AGGCCCCAAG GGAGAACCGG GCCCTGCTGG TCCCCAAGGA 
GCCCCTGGTC CTGCTGGTGA AGAAGGGAAA AGAGGTGCCC GTGGAGAACC TGGTGGTGCT 
GGGCCCGCCG GTCCCCCTGG AGAAAGAGGC GCTCCTGGAA ACCGTGGTTT CCCAGGTCAG 
GATGGTCTGG CAGGTCCCAA GGGAGCCCCT GGAGAGCGAG GACCCAGTGG CCTCGCTGGT 
CCCAAAGGCG CCAACGGTGA CCCTGGCCGT CCCGGAGAGC CTGGCCTTCC TGGAGCCCGG 
GGTCTCACTG GTCGCCCTGG TGATGCTGGT CCTCAAGGCA AAGTTGGTCC TTCCGGAGCC 
CCTGGTGAAG ACGGTCGCCC TGGACCCCCA GGTCCTCAGG GGGCTCGTGG GCAGCCTGGT 
GTCATGGGTT TCCCTGGCCC CAAAGGTGCC AATGGTGAGC CTGGCAAAGC TGGTGAGAAA 
GGACTGCCTG GTGCTCCTGG TCTGAGAGGT CTTCCTGGCA AAGATGGTGA GACAGGTGCT 
GCAGGCCCCC CTGGACCCGC TGGACCAGCT GGTGAACGAG GCGAGCAGGG TGCTCCTGGG 
CCATCTGGGT TCCAGGGACT TCCTGGCCCT CCCGGCCCCC CAGGTGAAGG TGGAAAACCA 
GGTGACCAGG GTGTTCCTGG TGAAGCTGGA GCTCCCGGCC TCGTGGGTCC CAGGGGTGAA 
CGAGGTTTCC CAGGAGAACG TGGCTCCCCC GGTTCCCAGG GCCTCCAGGG GGCCCGCGGT 
CTCCCCGGCA CTCCTGGCAC TGATGGTCCC AAAGGCGCAG CTGGCCCAGC TGGCCCCCCT 
GGGGCTCAGG GCCCTCCAGG TCTGCAGGGC ATGCCCGGTG AGAGGGGAGC AGCTGGTATC 
GCCGGACCCA AGGGAGACAG GGGTGATGTT GGTGAGAAAG GCCCCGAGGG CGCCCCCGGG 
AAGGACGGTG GACGAGGTCT GACTGGCCCC ATTGGCCCCC CTGGCCCAGC CGGAGCCAAC 
GGTGAGAAGG GAGAAGTTGG ACCCCCTGGT CCTGCTGGAA CTGCTGGTGC TCGAGGTGCC 
CCGGGTGAAC GTGGAGAGAC AGGCCCCCCT GGACCTGCTG GATTCGCCGG TCCCCCTGGT 
GCTGATGGCC AGCCTGGTGC CAAAGGTGAA CAAGGAGAAG CCGGCCAGAA AGGTGACGCT 
GGTGCCCCCG GTCCTCAGGG CCCCTCTGGA GCTCCTGGGC CTCAGGGTCC TACTGGTGTG 
ACCGGTCCTA AAGGAGCCCG AGGTGCTCAA GGGCCCCCGG GAGCCACCGG ATTCCCCGGA 
GCAGCTGGCC GCGTCGGACC CCCAGGCTCC AATGGCAACC CTGGACCCCC TGGTCCTCCT 
GGTCCTTCTG GAAAAGATGG TCCTAAAGGT GCTCGAGGCG ACAGTGGGCC CCCTGGCCGA 
GCTGGTGACC CTGGCCTCCA AGGTCCTGCT GGACCCCCTG GCGAGAAGGG AGAGCCTGGA 
GATGATGGTC CTTCTGGTCC CGATGGTCCT CCAGGTCCTC AGGGTCTGGC TGGTCAGAGG 
GGCATCGTTG GTCTGCCTGG ACAGCGTGGT GAGCGAGGAT TCCCCGGCCT CCCCGGCCCC 
TCGGGTGAGC CTGGCAAGCA GGGAGCCCCT GGAGCATCTG GAGACCGAGG TCCCCCTGGC 
CCTGTGGGTC CTCCTGGCCT GACTGGTCCT GCTGGTGAGC CTGGACGTGA GGGAAGCCCC 
GGTGCTGACG GCCCCCCTGG CAGAGATGGC GCAGCTGGAG TCAAAGGTGA CCGTGGAGAG 
ACCGGTGCCG TGGGTGCTCC TGGAGCCCCT GGGCCCCCTG GCTCTCCTGG CCCCGCTGGC 
CCAATTGGCA AGCAGGGAGA CCGAGGAGAA GCTGGCGCAC AAGGTCCCAT GGGACCTGCA 
GGACCTGCTG GAGCCCGGGG AATGCCAGGC CCTCAAGGCC CCCGAGGTGA CAAAGGAGAA 
ACTGGAGAGG CTGGCGAGAG GGGACTGAAG GGACACCGTG GCTTCACTGG TCTGCAGGGT 
CTGCCCGGCC CCCCTGGTCC TTCTGGAGAC CAGGGTGCTT CCGGTCCTGC AGGTCCTTCC 
GGCCCCAGAG GTCCTCCTGG TCCCGTCGGT CCCTCTGGCA AAGATGGTGC TAACGGAATC 
CCTGGCCCCA TTGGACCTCC TGGGCCCCGT GGACGTTCCG GCGAGACTGG CCCTGCTGGT 
CCTCCTGGAA ACCCTGGACC CCCTGGCCCT CCTGGCCCCC CCGGCCCTGG CATCGACATG 
TCCGCCTTTG CTGGCCTTGG CCAGAGAGAG AAAGGCCCCG ATCCTCTGCA GTACATGCGG 
GCCGACGAGG CAGCCGGCAA CCTGAGACAG CATGATGCCG AGGTGGACGC CACACTCAAG 
TCCCTCAACA ACCAGATCGA GAGCCTCCGT AGCCCCGAGG GCTCCCGCAA GAACCCAGCT 
CGCACCTGCC GAGACCTGAA ACTCTGCCAC CCTGAGTGGA AGAGCGGAGA CTACTGGATC 
GACCCCAACC AGGGCTGCAC CCTGGATGCC ATGAAGGTTT TCTGCAACAT GGAGACTGGC 
GAGACCTGCG TCTACCCCAA CCCGGCCAGC GTCCCCAAGA AGAACTGGTG GAGCAGCAAG 
AGCAAGGACA AGAAACACAT CTGGTTTGGA GAAACCATCA ACGGTGGCTT CCACTTCAGC 
TATGGAGATG ACAACCTGGC TCCCAACACC GCCAACGTCC AGATGACCTT CCTGCGCCTG 
CTGTCCACCG AGGGCTCTCA GAACATCACC TACCACTGCA AGAACAGCAT TGCCTACCTG 
GACGAAGCTG CTGGCAACCT CAAGAAGGCT CTGCTCATCC AGGGCTCCAA CGACGTGGAG 
ATCCGGGCTG AGGGCAACAG CAGGTTCACA TATACCGTTC TGAAGGATGG CTGCACGAAA 
CACACCGGTA AGTGGGGCAA GACTATGATC GAGTACCGGT CACAGAAGAC CTCCCGTCTG 
CCCATCATTG ACATTGCACC CATGGACATA GGAGGGCCCG AGCAGGAATT CGGTGTGGAC 
ATAGGGCCTG TCTGCTTCTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001135.2
CDS	27..4490
Translation	MIRLGAPQTLVLLTLLVAAVLRCHGQDVQKAGSCVQDGQRYNDKDVWKPEPCRICVCDTGTVLCDDIICEDMKDCLSPETPFGECCPICSADLPTASGQPGPKGQKGEPGDIKDIVGPKGPPGPQGPAGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGPPGPPGPPGPPGLGGNFAAQMAGGFDEKAGGAQMGVMQGPMGPMGPRGPPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPPGKPGDDGEAGKPGKSGERGPPGPQGARGFPGTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDGQPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGPRGEPGTPGSPGPAGAAGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEPGIAGFKGEQGPKGEPGPAGPQGAPGPAGEEGKRGARGEPGGAGPAGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLAGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLPGAPGLRGLPGKDGETGAAGPPGPAGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKPGDQGVPGEAGAPGLVGPRGERGFPGERGSPGSQGLQGARGLPGTPGTDGPKGAAGPAGPPGAQGPPGLQGMPGERGAAGIAGPKGDRGDVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPAGTAGARGAPGERGETGPPGPAGFAGPPGADGQPGAKGEQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGSNGNPGPPGPPGPSGKDGPKGARGDSGPPGRAGDPGLQGPAGPPGEKGEPGDDGPSGPDGPPGPQGLAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGRDGAAGVKGDRGETGAVGAPGAPGPPGSPGPAGPIGKQGDRGEAGAQGPMGPAGPAGARGMPGPQGPRGDKGETGEAGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGANGIPGPIGPPGPRGRSGETGPAGPPGNPGPPGPPGPPGPGIDMSAFAGLGQREKGPDPLQYMRADEAAGNLRQHDAEVDATLKSLNNQIESLRSPEGSRKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPASVPKKNWWSSKSKDKKHIWFGETINGGFHFSYGDDNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEIRAEGNSRFTYTVLKDGCTKHTGKWGKTMIEYRSQKTSRLPIIDIAPMDIGGPEQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	NM_001001135.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type II alpha 1 chain (COL2A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001135.3

ORF Insert Sequence:

ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC 
CTTCGGTGTC ACGGCCAGGA TGTCCAGAAG GCTGGCAGCT GTGTGCAGGA CGGGCAGAGG 
TATAATGATA AGGATGTGTG GAAGCCCGAG CCCTGCCGGA TCTGTGTCTG TGACACTGGG 
ACTGTCCTCT GCGACGACAT AATCTGTGAA GACATGAAAG ACTGCCTCAG CCCCGAGACC 
CCCTTCGGAG AGTGCTGCCC CATCTGCTCA GCTGACCTCC CCACTGCCAG TGGGCAACCA 
GGACCAAAGG GACAGAAAGG AGAACCTGGA GATATCAAGG ACATCGTAGG ACCCAAGGGA 
CCTCCTGGAC CTCAGGGACC CGCAGGTGAA CAAGGACCCA GAGGTGATCG TGGTGACAAA 
GGTGAAAAAG GTGCTCCTGG ACCTCGTGGC AGAGATGGAG AGCCTGGGAC CCCTGGAAAC 
CCTGGCCCCC CTGGTCCTCC CGGCCCCCCT GGCCCCCCTG GCCTTGGTGG AAACTTTGCT 
GCCCAGATGG CTGGTGGATT TGATGAGAAG GCTGGTGGCG CCCAGATGGG AGTAATGCAG 
GGACCAATGG GCCCCATGGG ACCTCGGGGA CCCCCAGGCC CTGCTGGTGC TCCCGGACCT 
CAAGGATTTC AAGGCAACCC TGGTGAACCT GGTGAACCCG GTGTTTCTGG TCCCATGGGC 
CCCCGTGGTC CTCCTGGCCC CCCTGGGAAA CCTGGTGATG ATGGTGAAGC TGGAAAGCCT 
GGAAAATCTG GCGAGAGAGG CCCTCCTGGC CCTCAGGGTG CTCGTGGTTT CCCAGGAACC 
CCAGGCCTTC CTGGTGTCAA AGGTCACAGA GGTTACCCAG GTCTAGATGG TGCTAAGGGA 
GAAGCTGGTG CTCCAGGTGT GAAGGGTGAG AGTGGTTCCC CAGGTGAGAA CGGTTCTCCA 
GGCCCAATGG GTCCCCGCGG CTTGCCTGGT GAGAGAGGAC GGACTGGCCC TGCCGGCGCT 
GCAGGTGCTC GAGGCAATGA CGGTCAGCCA GGCCCTGCAG GGCCTCCGGG TCCTGTGGGT 
CCTGCTGGCG GTCCTGGCTT TCCTGGTGCT CCTGGTGCCA AGGGTGAAGC TGGCCCCACC 
GGTGCTCGAG GTCCCGAAGG CGCCCAGGGT CCTCGCGGTG AACCGGGTAC TCCTGGGTCC 
CCTGGGCCAG CTGGTGCTGC TGGCAACCCT GGAACTGATG GAATCCCTGG AGCCAAGGGA 
TCTGCTGGTG CCCCTGGCAT TGCTGGTGCT CCCGGCTTCC CTGGACCCCG TGGTCCACCC 
GGCCCTCAAG GTGCAACTGG TCCTCTGGGC CCAAAAGGTC AAACGGGTGA GCCTGGTATT 
GCTGGCTTCA AAGGCGAACA AGGCCCCAAG GGAGAACCGG GCCCTGCTGG TCCCCAAGGA 
GCCCCTGGTC CTGCTGGTGA AGAAGGGAAA AGAGGTGCCC GTGGAGAACC TGGTGGTGCT 
GGGCCCGCCG GTCCCCCTGG AGAAAGAGGC GCTCCTGGAA ACCGTGGTTT CCCAGGTCAG 
GATGGTCTGG CAGGTCCCAA GGGAGCCCCT GGAGAGCGAG GACCCAGTGG CCTCGCTGGT 
CCCAAAGGCG CCAACGGTGA CCCTGGCCGT CCCGGAGAGC CTGGCCTTCC TGGAGCCCGG 
GGTCTCACTG GTCGCCCTGG TGATGCTGGT CCTCAAGGCA AAGTTGGTCC TTCCGGAGCC 
CCTGGTGAAG ACGGTCGCCC TGGACCCCCA GGTCCTCAGG GGGCTCGTGG GCAGCCTGGT 
GTCATGGGTT TCCCTGGCCC CAAAGGTGCC AATGGTGAGC CTGGCAAAGC TGGTGAGAAA 
GGACTGCCTG GTGCTCCTGG TCTGAGAGGT CTTCCTGGCA AAGATGGTGA GACAGGTGCT 
GCAGGCCCCC CTGGACCCGC TGGACCAGCT GGTGAACGAG GCGAGCAGGG TGCTCCTGGG 
CCATCTGGGT TCCAGGGACT TCCTGGCCCT CCCGGCCCCC CAGGTGAAGG TGGAAAACCA 
GGTGACCAGG GTGTTCCTGG TGAAGCTGGA GCTCCCGGCC TCGTGGGTCC CAGGGGTGAA 
CGAGGTTTCC CAGGAGAACG TGGCTCCCCC GGTTCCCAGG GCCTCCAGGG GGCCCGCGGT 
CTCCCCGGCA CTCCTGGCAC TGATGGTCCC AAAGGCGCAG CTGGCCCAGC TGGCCCCCCT 
GGGGCTCAGG GCCCTCCAGG TCTGCAGGGC ATGCCCGGTG AGAGGGGAGC AGCTGGTATC 
GCCGGACCCA AGGGAGACAG GGGTGATGTT GGTGAGAAAG GCCCCGAGGG CGCCCCCGGG 
AAGGACGGTG GACGAGGTCT GACTGGCCCC ATTGGCCCCC CTGGCCCAGC CGGAGCCAAC 
GGTGAGAAGG GAGAAGTTGG ACCCCCTGGT CCTGCTGGAA CTGCTGGTGC TCGAGGTGCC 
CCGGGTGAAC GTGGAGAGAC AGGCCCCCCT GGACCTGCTG GATTCGCCGG TCCCCCTGGT 
GCTGATGGCC AGCCTGGTGC CAAAGGTGAA CAAGGAGAAG CCGGCCAGAA AGGTGACGCT 
GGTGCCCCCG GTCCTCAGGG CCCCTCTGGA GCTCCTGGGC CTCAGGGTCC TACTGGTGTG 
ACCGGTCCTA AAGGAGCCCG AGGTGCTCAA GGGCCCCCGG GAGCCACCGG ATTCCCCGGA 
GCAGCTGGCC GCGTCGGACC CCCAGGCTCC AATGGCAACC CTGGACCCCC TGGTCCTCCT 
GGTCCTTCTG GAAAAGATGG TCCTAAAGGT GCTCGAGGCG ACAGTGGGCC CCCTGGCCGA 
GCTGGTGACC CTGGCCTCCA AGGTCCTGCT GGACCCCCTG GCGAGAAGGG AGAGCCTGGA 
GATGATGGTC CTTCTGGTCC CGATGGTCCT CCAGGTCCTC AGGGTCTGGC TGGTCAGAGG 
GGCATCGTTG GTCTGCCTGG ACAGCGTGGT GAGCGAGGAT TCCCCGGCCT CCCCGGCCCC 
TCGGGTGAGC CTGGCAAGCA GGGAGCCCCT GGAGCATCTG GAGACCGAGG TCCCCCTGGC 
CCTGTGGGTC CTCCTGGCCT GACTGGTCCT GCTGGTGAGC CTGGACGTGA GGGAAGCCCC 
GGTGCTGACG GCCCCCCTGG CAGAGATGGC GCAGCTGGAG TCAAAGGTGA CCGTGGAGAG 
ACCGGTGCCG TGGGTGCTCC TGGAGCCCCT GGGCCCCCTG GCTCTCCTGG CCCCGCTGGC 
CCAATTGGCA AGCAGGGAGA CCGAGGAGAA GCTGGCGCAC AAGGTCCCAT GGGACCTGCA 
GGACCTGCTG GAGCCCGGGG AATGCCAGGC CCTCAAGGCC CCCGAGGTGA CAAAGGAGAA 
ACTGGAGAGG CTGGCGAGAG GGGACTGAAG GGACACCGTG GCTTCACTGG TCTGCAGGGT 
CTGCCCGGCC CCCCTGGTCC TTCTGGAGAC CAGGGTGCTT CCGGTCCTGC AGGTCCTTCC 
GGCCCCAGAG GTCCTCCTGG TCCCGTCGGT CCCTCTGGCA AAGATGGTGC TAACGGAATC 
CCTGGCCCCA TTGGACCTCC TGGGCCCCGT GGACGTTCCG GCGAGACTGG CCCTGCTGGT 
CCTCCTGGAA ACCCTGGACC CCCTGGCCCT CCTGGCCCCC CCGGCCCTGG CATCGACATG 
TCCGCCTTTG CTGGCCTTGG CCAGAGAGAG AAAGGCCCCG ATCCTCTGCA GTACATGCGG 
GCCGACGAGG CAGCCGGCAA CCTGAGACAG CATGATGCCG AGGTGGACGC CACACTCAAG 
TCCCTCAACA ACCAGATCGA GAGCCTCCGT AGCCCCGAGG GCTCCCGCAA GAACCCAGCT 
CGCACCTGCC GAGACCTGAA ACTCTGCCAC CCTGAGTGGA AGAGCGGAGA CTACTGGATC 
GACCCCAACC AGGGCTGCAC CCTGGATGCC ATGAAGGTTT TCTGCAACAT GGAGACTGGC 
GAGACCTGCG TCTACCCCAA CCCGGCCAGC GTCCCCAAGA AGAACTGGTG GAGCAGCAAG 
AGCAAGGACA AGAAACACAT CTGGTTTGGA GAAACCATCA ACGGTGGCTT CCACTTCAGC 
TATGGAGATG ACAACCTGGC TCCCAACACC GCCAACGTCC AGATGACCTT CCTGCGCCTG 
CTGTCCACCG AGGGCTCTCA GAACATCACC TACCACTGCA AGAACAGCAT TGCCTACCTG 
GACGAAGCTG CTGGCAACCT CAAGAAGGCT CTGCTCATCC AGGGCTCCAA CGACGTGGAG 
ATCCGGGCTG AGGGCAACAG CAGGTTCACA TATACCGTTC TGAAGGATGG CTGCACGAAA 
CACACCGGTA AGTGGGGCAA GACTATGATC GAGTACCGGT CACAGAAGAC CTCCCGTCTG 
CCCATCATTG ACATTGCACC CATGGACATA GGAGGGCCCG AGCAGGAATT CGGTGTGGAC 
ATAGGGCCTG TCTGCTTCTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lethal chondrodysplasia in a family of Holstein cattle is associated with a de novo splice site variant of COL2A1.
BMC veterinary research12100(2016 Jun)
Agerholm JS,Menzi F,McEvoy FJ,Jagannathan V,Drögemüller C

Definition of the native and denatured type II collagen binding site for fibronectin using a recombinant collagen system.
The Journal of biological chemistry289(8)4941-51(2014 Feb)
An B,Abbonante V,Yigit S,Balduini A,Kaplan DL,Brodsky B

Two subsystems of meniscal collagen and their different thermal stabilities.
Doklady. Biochemistry and biophysics444175-7(2012 May-Jun)
Ignatieva NY,Zakharkina OL,Lunin VV,Baratova LA,Grokhovskaya TE,Balalaeva IV,Sergeeva EA

Disc strain and resulting positive mRNA expression from application of a noninvasive treatment.
Spine36(14)E921-8(2011 Jun)
Desmoulin GT,Hewitt CR,Hunter CJ

Free axial vibrations at 0 to 200 Hz positively affect extracellular matrix messenger ribonucleic acid expression in bovine nucleus pulposi.
Spine35(15)1437-44(2010 Jul)
Desmoulin GT,Reno CR,Hunter CJ

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COL2A1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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