NDUFA6 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFA6
Entrez Gene ID	327670
Full Name	NADH:ubiquinone oxidoreductase subunit A6
Synonyms	B14
General protein information	Preferred Names NADH:ubiquinone oxidoreductase subunit A6 Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 CI-B14 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 6, 14kDa NADH-ubiquinone oxidoreductase B14 subunit complex I-B14
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024991398.1	XP_024847166.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 isoform X1
NM_175791.2	NP_786985.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NDUFA6	XP_005625856.1
Bos taurus (cattle)	NDUFA6	NP_786985.1
Danio rerio (zebrafish)	ndufa6	NP_957262.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012533	XP_306101.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003328	XP_314225.5
Homo sapiens (human)	NDUFA6	NP_002481.2
Mus musculus (house mouse)	Ndufa6	NP_080263.1
Rattus norvegicus (Norway rat)	Ndufa6	NP_001123977.1
Xenopus tropicalis (tropical clawed frog)	ndufa6	NP_001017354.1
Pan troglodytes (chimpanzee)	NDUFA6	NP_001065259.1
Gallus gallus (chicken)	NDUFA6	NP_001265037.1
Drosophila melanogaster (fruit fly)	CG7712	NP_001260877.1
Macaca mulatta (Rhesus monkey)	LOC709640	XP_001106675.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Lemma-Gray P, Valusová E, Carroll CA, Weintraub ST, Musatov A, Robinson NC
Analytical biochemistry382(2)116-21(2008 Nov)

Oxidative damage to mitochondrial complex I due to peroxynitrite: identification of reactive tyrosines by mass spectrometry.
Murray J, Taylor SW, Zhang B, Ghosh SS, Capaldi RA
The Journal of biological chemistry278(39)37223-30(2003 Sep)

Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme.
Sazanov LA, Peak-Chew SY, Fearnley IM, Walker JE
Biochemistry39(24)7229-35(2000 Jun)

Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction.
Walker JE, Arizmendi JM, Dupuis A, Fearnley IM, Finel M, Medd SM, Pilkington SJ, Runswick MJ, Skehel JM
Journal of molecular biology226(4)1051-72(1992 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NDUFA6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFA6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43102	NM_175791.2 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase subunit A6 (NDUFA6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OBa43102	XM_024991398.1 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase subunit A6 (NDUFA6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFA6 ( NM_175791.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_786985.1 Bos taurus NADH:ubiquinone oxidoreductase subunit A6 (NDUFA6), mRNA.

Price & Availability↑

CloneID	OBa43102
Clone ID Related Accession (Same CDS sequence)	XM_024991398.1 , NM_175791.2
Accession Version	NM_175791.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X63211.1. On Jun 3, 2003 this sequence version replaced NM_175791.1. ##Evidence-Data-START## Transcript exon combination :: X63211.1, CF614977.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145420, SAMN03145433 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCGA GCGGACTCCG CCAGGCTGCC GTTGCGGCCA GCACCTCGGT GAAGCCCATT 
TTCAGTCGGG ACATGAACGA GGCCAAGCGG AGGGTGCGCG AACTCTACCG CGCCTGGTAT 
CGGGAGGTGC CGAACACTGT GCATTTATTC CAGCTAGACA TCTCTGTGAA ACAGGGACGG 
GATAAAGTCC GGGAAATGTT TAAGAAGAAT GCGCACATCA CAGACCCCAG GGTGGTTGAT 
CTTCTGGTAA TTAAGGGAAA GATGGAACTG GAAGAAACAA TTAAAGTGTG GAAGCAGCGG 
ACACATGTTA TGCGGTTTTT CCATGAAACA GAAGCACCGA GGCCAAAGGA TTTCCTCTCC 
AAGTTCTATG TTGGGCACGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_786985.1
CDS	6..392
Translation	MAASGLRQAAVAASTSVKPIFSRDMNEAKRRVRELYRAWYREVPNTVHLFQLDISVKQGRDKVREMFKKNAHITDPRVVDLLVIKGKMELEETIKVWKQRTHVMRFFHETEAPRPKDFLSKFYVGHDP

Target ORF information:

RefSeq Version	NM_175791.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit A6 (NDUFA6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175791.2

ORF Insert Sequence:

ATGGCGGCGA GCGGACTCCG CCAGGCTGCC GTTGCGGCCA GCACCTCGGT GAAGCCCATT 
TTCAGTCGGG ACATGAACGA GGCCAAGCGG AGGGTGCGCG AACTCTACCG CGCCTGGTAT 
CGGGAGGTGC CGAACACTGT GCATTTATTC CAGCTAGACA TCTCTGTGAA ACAGGGACGG 
GATAAAGTCC GGGAAATGTT TAAGAAGAAT GCGCACATCA CAGACCCCAG GGTGGTTGAT 
CTTCTGGTAA TTAAGGGAAA GATGGAACTG GAAGAAACAA TTAAAGTGTG GAAGCAGCGG 
ACACATGTTA TGCGGTTTTT CCATGAAACA GAAGCACCGA GGCCAAAGGA TTTCCTCTCC 
AAGTTCTATG TTGGGCACGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFA6 ( XM_024991398.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847166.1 Bos taurus NADH:ubiquinone oxidoreductase subunit A6 (NDUFA6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa43102
Clone ID Related Accession (Same CDS sequence)	XM_024991398.1 , NM_175791.2
Accession Version	XM_024991398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA GCGGACTCCG CCAGGCTGCC GTTGCGGCCA GCACCTCGGT GAAGCCCATT 
TTCAGTCGGG ACATGAACGA GGCCAAGCGG AGGGTGCGCG AACTCTACCG CGCCTGGTAT 
CGGGAGGTGC CGAACACTGT GCATTTATTC CAGCTAGACA TCTCTGTGAA ACAGGGACGG 
GATAAAGTCC GGGAAATGTT TAAGAAGAAT GCGCACATCA CAGACCCCAG GGTGGTTGAT 
CTTCTGGTAA TTAAGGGAAA GATGGAACTG GAAGAAACAA TTAAAGTGTG GAAGCAGCGG 
ACACATGTTA TGCGGTTTTT CCATGAAACA GAAGCACCGA GGCCAAAGGA TTTCCTCTCC 
AAGTTCTATG TTGGGCACGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847166.1
CDS	69..455
Translation	MAASGLRQAAVAASTSVKPIFSRDMNEAKRRVRELYRAWYREVPNTVHLFQLDISVKQGRDKVREMFKKNAHITDPRVVDLLVIKGKMELEETIKVWKQRTHVMRFFHETEAPRPKDFLSKFYVGHDP

Target ORF information:

RefSeq Version	XM_024991398.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit A6 (NDUFA6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991398.1

ORF Insert Sequence:

ATGGCGGCGA GCGGACTCCG CCAGGCTGCC GTTGCGGCCA GCACCTCGGT GAAGCCCATT 
TTCAGTCGGG ACATGAACGA GGCCAAGCGG AGGGTGCGCG AACTCTACCG CGCCTGGTAT 
CGGGAGGTGC CGAACACTGT GCATTTATTC CAGCTAGACA TCTCTGTGAA ACAGGGACGG 
GATAAAGTCC GGGAAATGTT TAAGAAGAAT GCGCACATCA CAGACCCCAG GGTGGTTGAT 
CTTCTGGTAA TTAAGGGAAA GATGGAACTG GAAGAAACAA TTAAAGTGTG GAAGCAGCGG 
ACACATGTTA TGCGGTTTTT CCATGAAACA GAAGCACCGA GGCCAAAGGA TTTCCTCTCC 
AAGTTCTATG TTGGGCACGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Analytical biochemistry382(2)116-21(2008 Nov)
Lemma-Gray P,Valusová E,Carroll CA,Weintraub ST,Musatov A,Robinson NC

Oxidative damage to mitochondrial complex I due to peroxynitrite: identification of reactive tyrosines by mass spectrometry.
The Journal of biological chemistry278(39)37223-30(2003 Sep)
Murray J,Taylor SW,Zhang B,Ghosh SS,Capaldi RA

Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme.
Biochemistry39(24)7229-35(2000 Jun)
Sazanov LA,Peak-Chew SY,Fearnley IM,Walker JE

Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction.
Journal of molecular biology226(4)1051-72(1992 Aug)
Walker JE,Arizmendi JM,Dupuis A,Fearnley IM,Finel M,Medd SM,Pilkington SJ,Runswick MJ,Skehel JM