PYGM cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PYGM
Entrez Gene ID	327664
Full Name	glycogen phosphorylase, muscle associated
General protein information	Preferred Names glycogen phosphorylase, muscle associated Names glycogen phosphorylase, muscle form myophosphorylase phosphorylase, glycogen, muscle phosphorylase, glycogen; muscle (McArdle syndrome, glycogen storage disease type V)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_175786.2	NP_786980.1	glycogen phosphorylase, muscle form

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007939	XP_317541.3
Macaca mulatta (Rhesus monkey)	PYGM	XP_001114805.1
Oryza sativa (rice)	Os01g0851700	NP_001044823.1
Homo sapiens (human)	PYGM	NP_005600.1
Canis lupus familiaris (dog)	PYGM	XP_853123.1
Rattus norvegicus (Norway rat)	Pygm	NP_036770.1
Drosophila melanogaster (fruit fly)	GlyP	NP_722762.1
Xenopus tropicalis (tropical clawed frog)	LOC100491051	XP_002935410.2
Arabidopsis thaliana (thale cress)	PHS2	NP_190281.1
Bos taurus (cattle)	PYGM	NP_786980.1
Mus musculus (house mouse)	Pygm	NP_035354.1
Danio rerio (zebrafish)	pygma	NP_001018464.1
Caenorhabditis elegans (roundworm)	T22F3.3	NP_504007.1
Pan troglodytes (chimpanzee)	PYGM	XP_001165783.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Cloning of bovine muscle glycogen phosphorylase cDNA and identification of a mutation in cattle with myophosphorylase deficiency, an animal model for McArdle's disease.
Tsujino S, Shanske S, Valberg SJ, Cardinet GH 3rd, Smith BP, DiMauro S
Neuromuscular disorders : NMD6(1)19-26(1996 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PYGM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PYGM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42290	NM_175786.2 Latest version!	Bos taurus glycogen phosphorylase, muscle associated (PYGM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PYGM ( NM_175786.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_786980.1 Bos taurus glycogen phosphorylase, muscle associated (PYGM), mRNA.

Price & Availability↑

CloneID	OBa42290
Clone ID Related Accession (Same CDS sequence)	NM_175786.2
Accession Version	NM_175786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577030400000
Organism	Bos taurus(cattle)
Product	glycogen phosphorylase, muscle form
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S82859.1. On or before Mar 29, 2005 this sequence version replaced XM_615171.1, NM_175786.1. ##Evidence-Data-START## Transcript exon combination :: S82859.1, BC151314.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145459, SAMN03145475 [ECO:0000348] ##Evidence-Data-END##

ATGTCCCGGC CCCTGACAGA CCAGGAGAAG AGAAAGCAGA TCAGCGTGCG TGGCCTGGCC 
GGTGTGGAGA ATGTGACTGA GCTGAAGAAG AACTTCAACC GGCACCTACA CTTCACCCTT 
GTGAAGGACC GCAATGTGGC CACCCCGCGA GACTACTACT TCGCGCTGGC CTACACGGTG 
CGAGACCACC TCGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GAAGGACCCC 
AAGAGGATCT ACTACCTGTC TCTGGAATTC TACATCGGCC GGACGCTGCA GAACACCATG 
GTGAACCTGG CGTTGGAGAA TGCCTGTGAC GAGGCCACCT ATCAGCTGGG CCTAGACATG 
GAGGAGCTGG AGGAAATCGA GGAGGATGCA GGGCTGGGCA ACGGGGGCCT GGGCCGGCTG 
GCGGCCTGCT TTCTGGACTC AATGGCAACA CTGGGCCTGG CTGCCTATGG CTATGGGATC 
CGCTATGAGT TTGGGATTTT TAACCAGAAG ATCTCTGGGG GTTGGCAGAT GGAGGAAGCT 
GATGACTGGC TTCGCTACGG CAACCCCTGG GAGAAGGCCC GTCCTGAGTT CACGCTGCCG 
GTGCACTTCT ATGGCCGAGT GGAACACACC AGCCAGGGGG CCAAGTGGGT GGACACACAG 
GTGGTGCTGG CCATGCCCTA CGACACACCC GTGCCTGGCT ACCGCAACAA CGTGGTCAAC 
ACCATGCGCC TGTGGTCTGC CAAAGCCCCC AACGACTTCA ACCTCAAGGA CTTCAATGTC 
GGTGGCTACA TCCAGGCCGT GCTGGACCGC AACCTGGCTG AGAACATCTC ACGGGTCCTG 
TACCCCAACG ACAACTTCTT TGAGGGGAAG GAGCTGCGGC TGAAGCAGGA GTACTTCGTA 
GTGGCTGCCA CGCTCCAGGA CATCATCCGC CGCTTCAAGT CCTCCAAGTT TGGCTGCCTC 
GACCCCGTGC GCACCAACTT CGATGCCTTC CCAGATAAGG TGGCCATCCA GCTCAATGAC 
ACCCACCCCT CCTTGGCCAT CCCTGAGCTG ATGAGGATTC TGGTGGACCA GGAGCGCCTG 
GAGTGGGAAA AGGCATGGGA AGTGACTGTG AAGACTTGTG CCTACACCAA CCACACGGTG 
CTGCCCGAGG CCCTGGAGCG CTGGCCTGTG CACCTCATAG AGACCCTGCT GCCGCGGCAC 
CTCCAGATCA TCTATGAAAT CAACCAGCGC TTCCTTAACC GGGTGGCAGC CGCGTTCCCA 
GGGGATGTAG ACCGACTGCG GCGCATGTCG CTGGTGGAGG AGGGCGCGGT GAAGCGCATC 
AATATGGCAC ACCTGTGCAT CGCCGGGTCT CATGCCGTCA ATGGCGTGGC TCGCATCCAC 
TCGGAGATCC TCAAGAAGAC CATCTTCAAG GACTTCTACG AGCTGGAGCC TCATAAGTTC 
CAGAATAAGA CCAATGGGAT CACCCCTCGG CGCTGGTTGG TGATGTGTAA CCCTGGGCTG 
GCAGAGATCA TTGCTGAGCG CATCGGAGAG GAATACATCG CAGACCTGGA TCAGCTGCGC 
AAACTGCTGT CCTATGTGGA TGATGAATCC TTTATCCGGG ATGTGGCCAA AGTGAAGCAG 
GAAAACAAGT TAAAGTTTTC TGCATACCTG GAGAAGGAAT ACAAAGTCCA CATCAACCCC 
AACTCACTCT TCGATATCCA GGTGAAGCGG ATTCACGAAT ATAAACGCCA GCTCCTCAAC 
TGCCTCCATG TCATCACGCT GTACAACCGT ATCAAGAAGG AACCCAATAA GTTTTTTGTG 
CCTCGGACTG TGATGATTGG AGGGAAGGCT GCCCCTGGGT ACCATATGGC CAAGATGATC 
ATCAAACTCA TCACAGCCAT TGGGGATGTG GTCAACCACG ACCCGGTGGT GGGAGACCGC 
CTTCGTGTGA TCTTCCTGGA GAACTACCGA GTCTCGCTGG CCGAGAAAGT GATCCCGGCT 
GCTGACCTCT CCGAGCAGAT CTCTACTGCG GGCACTGAAG CCTCAGGCAC TGGCAACATG 
AAGTTCATGC TCAACGGGGC TCTGACCATT GGCACCATGG ACGGGGCCAA CGTGGAGATG 
GCGGAAGAGG CGGGAGAGGA GAACTTCTTC ATCTTTGGCA TGCGGGTGGA GGACGTGGAA 
AGGCTTGACC AGAAAGGGTA CAATGCCCAG GAGTATTATG ACCGGATTCC TGAGCTTCGG 
CACGTCATTG ACCAGCTGAG CAGTGGCTTC TTCTCCCCCA AACAGCCTGA CCTATTCAAG 
GACATTGTCA ACATGCTCAT GCACCATGAC CGGTTTAAAG TCTTTGCGGA TTATGAAGAG 
TACATTAAAT GCCAGGAAAG AGTCAGTGCC TTGTACAAGA ACCCGAGAGA GTGGACGCGG 
ATGGTGATCC GGAACATAGC TACATCTGGC AAGTTCTCCA GTGACCGCAC CATTGCCCAG 
TACGCCAGGG AGATCTGGGG CGTGGAGCCG ACCCGCCAGC GCATGCCGGC CCCGGATGAG 
AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_786980.1
CDS	39..2567
Translation	MSRPLTDQEKRKQISVRGLAGVENVTELKKNFNRHLHFTLVKDRNVATPRDYYFALAYTVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYIGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKISGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGRVEHTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCLDPVRTNFDAFPDKVAIQLNDTHPSLAIPELMRILVDQERLEWEKAWEVTVKTCAYTNHTVLPEALERWPVHLIETLLPRHLQIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVMCNPGLAEIIAERIGEEYIADLDQLRKLLSYVDDESFIRDVAKVKQENKLKFSAYLEKEYKVHINPNSLFDIQVKRIHEYKRQLLNCLHVITLYNRIKKEPNKFFVPRTVMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPVVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVERLDQKGYNAQEYYDRIPELRHVIDQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEEYIKCQERVSALYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPTRQRMPAPDEKI

Target ORF information:

RefSeq Version	NM_175786.2
Organism	Bos taurus(cattle)
Definition	Bos taurus glycogen phosphorylase, muscle associated (PYGM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175786.2

ORF Insert Sequence:

ATGTCCCGGC CCCTGACAGA CCAGGAGAAG AGAAAGCAGA TCAGCGTGCG TGGCCTGGCC 
GGTGTGGAGA ATGTGACTGA GCTGAAGAAG AACTTCAACC GGCACCTACA CTTCACCCTT 
GTGAAGGACC GCAATGTGGC CACCCCGCGA GACTACTACT TCGCGCTGGC CTACACGGTG 
CGAGACCACC TCGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GAAGGACCCC 
AAGAGGATCT ACTACCTGTC TCTGGAATTC TACATCGGCC GGACGCTGCA GAACACCATG 
GTGAACCTGG CGTTGGAGAA TGCCTGTGAC GAGGCCACCT ATCAGCTGGG CCTAGACATG 
GAGGAGCTGG AGGAAATCGA GGAGGATGCA GGGCTGGGCA ACGGGGGCCT GGGCCGGCTG 
GCGGCCTGCT TTCTGGACTC AATGGCAACA CTGGGCCTGG CTGCCTATGG CTATGGGATC 
CGCTATGAGT TTGGGATTTT TAACCAGAAG ATCTCTGGGG GTTGGCAGAT GGAGGAAGCT 
GATGACTGGC TTCGCTACGG CAACCCCTGG GAGAAGGCCC GTCCTGAGTT CACGCTGCCG 
GTGCACTTCT ATGGCCGAGT GGAACACACC AGCCAGGGGG CCAAGTGGGT GGACACACAG 
GTGGTGCTGG CCATGCCCTA CGACACACCC GTGCCTGGCT ACCGCAACAA CGTGGTCAAC 
ACCATGCGCC TGTGGTCTGC CAAAGCCCCC AACGACTTCA ACCTCAAGGA CTTCAATGTC 
GGTGGCTACA TCCAGGCCGT GCTGGACCGC AACCTGGCTG AGAACATCTC ACGGGTCCTG 
TACCCCAACG ACAACTTCTT TGAGGGGAAG GAGCTGCGGC TGAAGCAGGA GTACTTCGTA 
GTGGCTGCCA CGCTCCAGGA CATCATCCGC CGCTTCAAGT CCTCCAAGTT TGGCTGCCTC 
GACCCCGTGC GCACCAACTT CGATGCCTTC CCAGATAAGG TGGCCATCCA GCTCAATGAC 
ACCCACCCCT CCTTGGCCAT CCCTGAGCTG ATGAGGATTC TGGTGGACCA GGAGCGCCTG 
GAGTGGGAAA AGGCATGGGA AGTGACTGTG AAGACTTGTG CCTACACCAA CCACACGGTG 
CTGCCCGAGG CCCTGGAGCG CTGGCCTGTG CACCTCATAG AGACCCTGCT GCCGCGGCAC 
CTCCAGATCA TCTATGAAAT CAACCAGCGC TTCCTTAACC GGGTGGCAGC CGCGTTCCCA 
GGGGATGTAG ACCGACTGCG GCGCATGTCG CTGGTGGAGG AGGGCGCGGT GAAGCGCATC 
AATATGGCAC ACCTGTGCAT CGCCGGGTCT CATGCCGTCA ATGGCGTGGC TCGCATCCAC 
TCGGAGATCC TCAAGAAGAC CATCTTCAAG GACTTCTACG AGCTGGAGCC TCATAAGTTC 
CAGAATAAGA CCAATGGGAT CACCCCTCGG CGCTGGTTGG TGATGTGTAA CCCTGGGCTG 
GCAGAGATCA TTGCTGAGCG CATCGGAGAG GAATACATCG CAGACCTGGA TCAGCTGCGC 
AAACTGCTGT CCTATGTGGA TGATGAATCC TTTATCCGGG ATGTGGCCAA AGTGAAGCAG 
GAAAACAAGT TAAAGTTTTC TGCATACCTG GAGAAGGAAT ACAAAGTCCA CATCAACCCC 
AACTCACTCT TCGATATCCA GGTGAAGCGG ATTCACGAAT ATAAACGCCA GCTCCTCAAC 
TGCCTCCATG TCATCACGCT GTACAACCGT ATCAAGAAGG AACCCAATAA GTTTTTTGTG 
CCTCGGACTG TGATGATTGG AGGGAAGGCT GCCCCTGGGT ACCATATGGC CAAGATGATC 
ATCAAACTCA TCACAGCCAT TGGGGATGTG GTCAACCACG ACCCGGTGGT GGGAGACCGC 
CTTCGTGTGA TCTTCCTGGA GAACTACCGA GTCTCGCTGG CCGAGAAAGT GATCCCGGCT 
GCTGACCTCT CCGAGCAGAT CTCTACTGCG GGCACTGAAG CCTCAGGCAC TGGCAACATG 
AAGTTCATGC TCAACGGGGC TCTGACCATT GGCACCATGG ACGGGGCCAA CGTGGAGATG 
GCGGAAGAGG CGGGAGAGGA GAACTTCTTC ATCTTTGGCA TGCGGGTGGA GGACGTGGAA 
AGGCTTGACC AGAAAGGGTA CAATGCCCAG GAGTATTATG ACCGGATTCC TGAGCTTCGG 
CACGTCATTG ACCAGCTGAG CAGTGGCTTC TTCTCCCCCA AACAGCCTGA CCTATTCAAG 
GACATTGTCA ACATGCTCAT GCACCATGAC CGGTTTAAAG TCTTTGCGGA TTATGAAGAG 
TACATTAAAT GCCAGGAAAG AGTCAGTGCC TTGTACAAGA ACCCGAGAGA GTGGACGCGG 
ATGGTGATCC GGAACATAGC TACATCTGGC AAGTTCTCCA GTGACCGCAC CATTGCCCAG 
TACGCCAGGG AGATCTGGGG CGTGGAGCCG ACCCGCCAGC GCATGCCGGC CCCGGATGAG 
AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Cloning of bovine muscle glycogen phosphorylase cDNA and identification of a mutation in cattle with myophosphorylase deficiency, an animal model for McArdle's disease.
Neuromuscular disorders : NMD6(1)19-26(1996 Jan)
Tsujino S,Shanske S,Valberg SJ,Cardinet GH 3rd,Smith BP,DiMauro S

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PYGM cDNA ORF clone, Bos taurus(cattle)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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