NDUFV1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFV1
Entrez Gene ID	287014
Full Name	NADH:ubiquinone oxidoreductase core subunit V1
General protein information	Preferred Names NADH:ubiquinone oxidoreductase core subunit V1 Names NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial CI-51kD NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa NADH dehydrogenase flavoprotein 1 NADH dehydrogenase ubiquinone flavoprotein 1 NADH-ubiquinone oxidoreductase 51 kDa subunit complex I-51kD
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010821048.2	XP_010819350.1	NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial isoform X1
XM_010821048.1	XP_010819350.1	NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial isoform X1
NM_174808.1	NP_777233.1	NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	NDUFV1	NP_009034.2
Pan troglodytes (chimpanzee)	NDUFV1	NP_001065293.1
Caenorhabditis elegans (roundworm)	nuo-1	NP_496376.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010039	XP_319184.4
Oryza sativa (rice)	Os07g0645400	NP_001060455.1
Magnaporthe oryzae (rice blast fungus)	MGG_00647	XP_003718370.1
Danio rerio (zebrafish)	ndufv1	NP_001003747.1
Canis lupus familiaris (dog)	NDUFV1	XP_851761.2
Bos taurus (cattle)	NDUFV1	NP_777233.1
Rattus norvegicus (Norway rat)	Ndufv1	NP_001006973.1
Drosophila melanogaster (fruit fly)	CG9140	NP_608987.1
Arabidopsis thaliana (thale cress)	CI51	NP_196470.1
Mus musculus (house mouse)	Ndufv1	NP_598427.1
Neurospora crassa	NCU04044	XP_957659.1
Gallus gallus (chicken)	NDUFV1	NP_001026754.1
Xenopus tropicalis (tropical clawed frog)	ndufv1	NP_001011491.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Lemma-Gray P, Valusová E, Carroll CA, Weintraub ST, Musatov A, Robinson NC
Analytical biochemistry382(2)116-21(2008 Nov)

Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme.
Sazanov LA, Peak-Chew SY, Fearnley IM, Walker JE
Biochemistry39(24)7229-35(2000 Jun)

cDNA-derived amino acid sequence of the NADH-binding 51-kDa subunit of the bovine respiratory NADH dehydrogenase reveals striking similarities to a bacterial NAD(+)-reducing hydrogenase.
Patel SD, Aebersold R, Attardi G
Proceedings of the National Academy of Sciences of the United States of America88(10)4225-9(1991 May)

Relationship between mitochondrial NADH-ubiquinone reductase and a bacterial NAD-reducing hydrogenase.
Pilkington SJ, Skehel JM, Gennis RB, Walker JE
Biochemistry30(8)2166-75(1991 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NDUFV1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFV1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41772	NM_174808.1 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase core subunit V1 (NDUFV1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa231846	XM_010821048.2 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase core subunit V1 (NDUFV1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa45534	XM_010821048.1 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase core subunit V1 (NDUFV1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFV1 ( NM_174808.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777233.1 Bos taurus NADH:ubiquinone oxidoreductase core subunit V1 (NDUFV1), mRNA.

Price & Availability↑

CloneID	OBa41772
Clone ID Related Accession (Same CDS sequence)	NM_174808.1
Accession Version	NM_174808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M58607.1. ##Evidence-Data-START## Transcript exon combination :: M58607.1, BC118304.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCTGGCGG CACGGCGGCT GCTCGGCGGG TCGCTCCCCG CGCGGGTGTC TGTGCGATTC 
AGCGGCGACA CGACAGCGCC CAAGAAAACC TCATTTGGCT CGCTGAAGGA CGAAGACCGG 
ATCTTCACCA ACCTGTATGG CCGCCATGAC TGGAGGCTTA AAGGTGCCCA GAGTCGAGGT 
GACTGGTACA AGACGAAGGA GATTCTGCTG AAGGGGCCTG ACTGGATCCT GGGTGAGGTC 
AAGACGTCGG GCTTGCGGGG CCGTGGAGGT GCTGGCTTCC CCACTGGCCT TAAATGGAGC 
TTCATGAATA AGCCCTCAGA TGGCAGGCCC AAGTATCTGG TGGTGAACGC GGATGAGGGG 
GAGCCAGGCA CGTGCAAGGA CCGAGAGATC ATCCGCCACG ACCCCCACAA GCTGGTGGAA 
GGCTGCCTAG TAGGGGGCCG GGCCATGGGC GCTCGTGCCG CCTACATCTA CATCCGCGGG 
GAGTTCTACA ACGAGGCCTC CAATCTGCAG GTAGCCATCC GAGAGGCCTA CGAGGCTGGT 
CTGATTGGCA AGAATGCCTG CGGCTCCGGC TACGATTTCG ACGTGTTTGT GGTGCGTGGG 
GCCGGGGCCT ACATCTGCGG GGAGGAGACG GCGCTCATCG AGTCCATCGA GGGCAAACAG 
GGCAAGCCCC GCCTGAAGCC GCCTTTCCCT GCAGACGTGG GAGTGTTTGG CTGCCCTACA 
ACTGTGGCCA ATGTGGAGAC TGTGGCCGTG TCCCCTACCA TCTGCCGCCG TGGGGGTGCC 
TGGTTTGCCA GCTTCGGCCG AGAGCGCAAC TCAGGCACCA AACTGTTCAA CATCTCTGGC 
CACGTCAACA ACCCCTGCAC CGTGGAGGAG GAGATGTCTG TGCCGCTGAA GGAGCTGATT 
GAAAAGCACG CCGGGGGTGT CACGGGTGGC TGGGACAACC TCCTTGCTGT GATCCCTGGC 
GGCTCGTCCA CCCCGCTGAT CCCCAAGTCC GTGTGTGAGA CGGTGCTGAT GGACTTCGAC 
GCGCTGATCC AGGCGCAGAC GGGGCTGGGC ACGGCTGCCG TGATCGTCAT GGACCGCTCG 
ACGGACATTG TGAAAGCCAT CGCCCGCCTC ATCGAGTTCT ACAAGCATGA GAGCTGTGGC 
CAGTGTACCC CGTGCCGCGA GGGTGTGGAC TGGATGAACA AGGTGATGGC CCGCTTTGTG 
AGGGGAGATG CCCGGCCGGC AGAGATCGAC TCTCTGTGTG AGATCAGCAA GCAGATCGAG 
GGCCACACCA TCTGCGCCCT GGGCGATGGG GCTGCGTGGC CCGTGCAGGG CCTGATCCGA 
CACTTCCGGC CGGAGCTCGA GGAGCGGATG CAGCAGTTTG CCCAGCAGCA CCAGGCCAGG 
CAAGCAGCTT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777233.1
CDS	59..1453
Translation	MLAARRLLGGSLPARVSVRFSGDTTAPKKTSFGSLKDEDRIFTNLYGRHDWRLKGAQSRGDWYKTKEILLKGPDWILGEVKTSGLRGRGGAGFPTGLKWSFMNKPSDGRPKYLVVNADEGEPGTCKDREIIRHDPHKLVEGCLVGGRAMGARAAYIYIRGEFYNEASNLQVAIREAYEAGLIGKNACGSGYDFDVFVVRGAGAYICGEETALIESIEGKQGKPRLKPPFPADVGVFGCPTTVANVETVAVSPTICRRGGAWFASFGRERNSGTKLFNISGHVNNPCTVEEEMSVPLKELIEKHAGGVTGGWDNLLAVIPGGSSTPLIPKSVCETVLMDFDALIQAQTGLGTAAVIVMDRSTDIVKAIARLIEFYKHESCGQCTPCREGVDWMNKVMARFVRGDARPAEIDSLCEISKQIEGHTICALGDGAAWPVQGLIRHFRPELEERMQQFAQQHQARQAAF

Target ORF information:

RefSeq Version	NM_174808.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase core subunit V1 (NDUFV1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174808.1

ORF Insert Sequence:

ATGCTGGCGG CACGGCGGCT GCTCGGCGGG TCGCTCCCCG CGCGGGTGTC TGTGCGATTC 
AGCGGCGACA CGACAGCGCC CAAGAAAACC TCATTTGGCT CGCTGAAGGA CGAAGACCGG 
ATCTTCACCA ACCTGTATGG CCGCCATGAC TGGAGGCTTA AAGGTGCCCA GAGTCGAGGT 
GACTGGTACA AGACGAAGGA GATTCTGCTG AAGGGGCCTG ACTGGATCCT GGGTGAGGTC 
AAGACGTCGG GCTTGCGGGG CCGTGGAGGT GCTGGCTTCC CCACTGGCCT TAAATGGAGC 
TTCATGAATA AGCCCTCAGA TGGCAGGCCC AAGTATCTGG TGGTGAACGC GGATGAGGGG 
GAGCCAGGCA CGTGCAAGGA CCGAGAGATC ATCCGCCACG ACCCCCACAA GCTGGTGGAA 
GGCTGCCTAG TAGGGGGCCG GGCCATGGGC GCTCGTGCCG CCTACATCTA CATCCGCGGG 
GAGTTCTACA ACGAGGCCTC CAATCTGCAG GTAGCCATCC GAGAGGCCTA CGAGGCTGGT 
CTGATTGGCA AGAATGCCTG CGGCTCCGGC TACGATTTCG ACGTGTTTGT GGTGCGTGGG 
GCCGGGGCCT ACATCTGCGG GGAGGAGACG GCGCTCATCG AGTCCATCGA GGGCAAACAG 
GGCAAGCCCC GCCTGAAGCC GCCTTTCCCT GCAGACGTGG GAGTGTTTGG CTGCCCTACA 
ACTGTGGCCA ATGTGGAGAC TGTGGCCGTG TCCCCTACCA TCTGCCGCCG TGGGGGTGCC 
TGGTTTGCCA GCTTCGGCCG AGAGCGCAAC TCAGGCACCA AACTGTTCAA CATCTCTGGC 
CACGTCAACA ACCCCTGCAC CGTGGAGGAG GAGATGTCTG TGCCGCTGAA GGAGCTGATT 
GAAAAGCACG CCGGGGGTGT CACGGGTGGC TGGGACAACC TCCTTGCTGT GATCCCTGGC 
GGCTCGTCCA CCCCGCTGAT CCCCAAGTCC GTGTGTGAGA CGGTGCTGAT GGACTTCGAC 
GCGCTGATCC AGGCGCAGAC GGGGCTGGGC ACGGCTGCCG TGATCGTCAT GGACCGCTCG 
ACGGACATTG TGAAAGCCAT CGCCCGCCTC ATCGAGTTCT ACAAGCATGA GAGCTGTGGC 
CAGTGTACCC CGTGCCGCGA GGGTGTGGAC TGGATGAACA AGGTGATGGC CCGCTTTGTG 
AGGGGAGATG CCCGGCCGGC AGAGATCGAC TCTCTGTGTG AGATCAGCAA GCAGATCGAG 
GGCCACACCA TCTGCGCCCT GGGCGATGGG GCTGCGTGGC CCGTGCAGGG CCTGATCCGA 
CACTTCCGGC CGGAGCTCGA GGAGCGGATG CAGCAGTTTG CCCAGCAGCA CCAGGCCAGG 
CAAGCAGCTT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFV1 ( XM_010821048.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819350.1 Bos taurus NADH:ubiquinone oxidoreductase core subunit V1 (NDUFV1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa231846
Clone ID Related Accession (Same CDS sequence)	XM_010821048.2
Accession Version	XM_010821048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010821048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCCT CGCGACAGGA GAAAGGCGGT CCGGAGGCCC ACGGGACTAC ATCTCCCAGC 
AGCCTCAGCG CCAGACAGGG CACTTCCGGC CTCTCCGCGC TCCCTCGAGG AGGCAGGGCC 
AAGGGCGGGG CGGGAGCGGC GCGGGGAAGT TGGGGCGCGC CCTGTCAGGG CGCGTGTGAC 
GTTCTTCCTG TGCGCCGCCT AGCGTCTCGG TCGTGCCGGG TTCTGAGCCT CAGAGCTGTG 
AAGACAGCGC CCAAGAAAAC CTCATTTGGC TCGCTGAAGG ACGAAGACCG GATCTTCACC 
AACCTGTATG GCCGCCATGA CTGGAGGCTT AAAGGTGCCC AGAGTCGAGG TGACTGGTAC 
AAGACGAAGG AGATTCTGCT GAAGGGGCCT GACTGGATCC TGGGTGAGGT CAAGACGTCG 
GGCTTGCGGG GCCGTGGAGG TGCTGGCTTC CCCACTGGCC TTAAATGGAG CTTCATGAAT 
AAGCCCTCAG ATGGCAGGCC CAAGTATCTG GTGGTGAACG CGGATGAGGG GGAGCCAGGC 
ACGTGCAAGG ACCGAGAGAT CATCCGCCAC GACCCCCACA AGCTGGTGGA AGGCTGCCTA 
GTAGGGGGCC GGGCCATGGG CGCTCGTGCC GCCTACATCT ACATCCGCGG GGAGTTCTAC 
AACGAGGCCT CCAATCTGCA GGTAGCCATC CGAGAGGCCT ACGAGGCTGG TCTGATTGGC 
AAGAATGCCT GCGGCTCCGG CTACGATTTC GACGTGTTTG TGGTGCGTGG GGCCGGGGCC 
TACATCTGCG GGGAGGAGAC GGCGCTCATC GAGTCCATCG AGGGCAAACA GGGCAAGCCC 
CGCCTGAAGC CGCCTTTCCC TGCAGACGTG GGAGTGTTTG GCTGCCCTAC AACTGTGGCC 
AATGTGGAGA CTGTGGCCGT GTCCCCTACC ATCTGCCGCC GTGGGGGTGC CTGGTTTGCC 
AGCTTCGGCC GAGAGCGCAA CTCAGGCACC AAACTGTTCA ACATCTCTGG CCACGTCAAC 
AACCCCTGCA CCGTGGAGGA GGAGATGTCT GTGCCGCTGA AGGAGCTGAT TGAAAAGCAC 
GCCGGGGGTG TCACGGGTGG CTGGGACAAC CTCCTTGCTG TGATCCCTGG CGGCTCGTCC 
ACCCCGCTGA TCCCCAAGTC CGTGTGTGAG ACGGTGCTGA TGGACTTCGA CGCGCTGATC 
CAGGCGCAGA CGGGGCTGGG CACGGCTGCC GTGATCGTCA TGGACCGCTC GACGGACATT 
GTGAAAGCCA TCGCCCGCCT CATCGAGTTC TACAAGCATG AGAGCTGTGG CCAGTGTACC 
CCGTGCCGCG AGGGTGTGGA CTGGATGAAC AAGGTGATGG CCCGCTTTGT GAGGGGAGAT 
GCCCGGCCGG CAGAGATCGA CTCTCTGTGG GAGATCAGCA AGCAGATCGA GGGCCACACC 
ATCTGCGCCC TGGGCGATGG GGCTGCGTGG CCCGTGCAGG GCCTGATCCG ACACTTCCGG 
CCGGAGCTCG AGGAGCGGAT GCAGCAGTTT GCCCAGCAGC ACCAGGCCAG GCAAGCAGCT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819350.1
CDS	148..1713
Translation	MPASRQEKGGPEAHGTTSPSSLSARQGTSGLSALPRGGRAKGGAGAARGSWGAPCQGACDVLPVRRLASRSCRVLSLRAVKTAPKKTSFGSLKDEDRIFTNLYGRHDWRLKGAQSRGDWYKTKEILLKGPDWILGEVKTSGLRGRGGAGFPTGLKWSFMNKPSDGRPKYLVVNADEGEPGTCKDREIIRHDPHKLVEGCLVGGRAMGARAAYIYIRGEFYNEASNLQVAIREAYEAGLIGKNACGSGYDFDVFVVRGAGAYICGEETALIESIEGKQGKPRLKPPFPADVGVFGCPTTVANVETVAVSPTICRRGGAWFASFGRERNSGTKLFNISGHVNNPCTVEEEMSVPLKELIEKHAGGVTGGWDNLLAVIPGGSSTPLIPKSVCETVLMDFDALIQAQTGLGTAAVIVMDRSTDIVKAIARLIEFYKHESCGQCTPCREGVDWMNKVMARFVRGDARPAEIDSLWEISKQIEGHTICALGDGAAWPVQGLIRHFRPELEERMQQFAQQHQARQAAF

Target ORF information:

RefSeq Version	XM_010821048.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase core subunit V1 (NDUFV1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010821048.2

ORF Insert Sequence:

ATGCCAGCCT CGCGACAGGA GAAAGGCGGT CCGGAGGCCC ACGGGACTAC ATCTCCCAGC 
AGCCTCAGCG CCAGACAGGG CACTTCCGGC CTCTCCGCGC TCCCTCGAGG AGGCAGGGCC 
AAGGGCGGGG CGGGAGCGGC GCGGGGAAGT TGGGGCGCGC CCTGTCAGGG CGCGTGTGAC 
GTTCTTCCTG TGCGCCGCCT AGCGTCTCGG TCGTGCCGGG TTCTGAGCCT CAGAGCTGTG 
AAGACAGCGC CCAAGAAAAC CTCATTTGGC TCGCTGAAGG ACGAAGACCG GATCTTCACC 
AACCTGTATG GCCGCCATGA CTGGAGGCTT AAAGGTGCCC AGAGTCGAGG TGACTGGTAC 
AAGACGAAGG AGATTCTGCT GAAGGGGCCT GACTGGATCC TGGGTGAGGT CAAGACGTCG 
GGCTTGCGGG GCCGTGGAGG TGCTGGCTTC CCCACTGGCC TTAAATGGAG CTTCATGAAT 
AAGCCCTCAG ATGGCAGGCC CAAGTATCTG GTGGTGAACG CGGATGAGGG GGAGCCAGGC 
ACGTGCAAGG ACCGAGAGAT CATCCGCCAC GACCCCCACA AGCTGGTGGA AGGCTGCCTA 
GTAGGGGGCC GGGCCATGGG CGCTCGTGCC GCCTACATCT ACATCCGCGG GGAGTTCTAC 
AACGAGGCCT CCAATCTGCA GGTAGCCATC CGAGAGGCCT ACGAGGCTGG TCTGATTGGC 
AAGAATGCCT GCGGCTCCGG CTACGATTTC GACGTGTTTG TGGTGCGTGG GGCCGGGGCC 
TACATCTGCG GGGAGGAGAC GGCGCTCATC GAGTCCATCG AGGGCAAACA GGGCAAGCCC 
CGCCTGAAGC CGCCTTTCCC TGCAGACGTG GGAGTGTTTG GCTGCCCTAC AACTGTGGCC 
AATGTGGAGA CTGTGGCCGT GTCCCCTACC ATCTGCCGCC GTGGGGGTGC CTGGTTTGCC 
AGCTTCGGCC GAGAGCGCAA CTCAGGCACC AAACTGTTCA ACATCTCTGG CCACGTCAAC 
AACCCCTGCA CCGTGGAGGA GGAGATGTCT GTGCCGCTGA AGGAGCTGAT TGAAAAGCAC 
GCCGGGGGTG TCACGGGTGG CTGGGACAAC CTCCTTGCTG TGATCCCTGG CGGCTCGTCC 
ACCCCGCTGA TCCCCAAGTC CGTGTGTGAG ACGGTGCTGA TGGACTTCGA CGCGCTGATC 
CAGGCGCAGA CGGGGCTGGG CACGGCTGCC GTGATCGTCA TGGACCGCTC GACGGACATT 
GTGAAAGCCA TCGCCCGCCT CATCGAGTTC TACAAGCATG AGAGCTGTGG CCAGTGTACC 
CCGTGCCGCG AGGGTGTGGA CTGGATGAAC AAGGTGATGG CCCGCTTTGT GAGGGGAGAT 
GCCCGGCCGG CAGAGATCGA CTCTCTGTGG GAGATCAGCA AGCAGATCGA GGGCCACACC 
ATCTGCGCCC TGGGCGATGG GGCTGCGTGG CCCGTGCAGG GCCTGATCCG ACACTTCCGG 
CCGGAGCTCG AGGAGCGGAT GCAGCAGTTT GCCCAGCAGC ACCAGGCCAG GCAAGCAGCT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFV1 ( XM_010821048.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819350.1 Bos taurus NADH:ubiquinone oxidoreductase core subunit V1 (NDUFV1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45534
Clone ID Related Accession (Same CDS sequence)	XM_010821048.1
Accession Version	XM_010821048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCCT CGCGACAGGA GAAAGGCGGT CCGGAGGCCC ACGGGACTAC ATCTCCCAGC 
AGCCTCAGCG CCAGACAGGG CACTTCCGGC CTCTCCGCGC TCCCTCGAGG AGGCAGGGCC 
AAGGGCGGGG CGGGAGCGGC GCGGGGAAGT TGGGGCGCGC CCTGTCAGGG CGCGTGTGAC 
GTTCTTCCTG TGCGCCGCCT AGCGTCTCGG TCGTGCCGGG TTCTGAGCCT CAGAGCTGTG 
AAGACAGCGC CCAAGAAAAC CTCATTTGGC TCGCTGAAGG ACGAAGACCG GATCTTCACC 
AACCTGTATG GCCGCCATGA CTGGAGGCTT AAAGGTGCCC AGAGTCGAGG TGACTGGTAC 
AAGACGAAGG AGATTCTGCT GAAGGGGCCT GACTGGATCC TGGGTGAGGT CAAGACGTCG 
GGCTTGCGGG GCCGTGGAGG TGCTGGCTTC CCCACTGGCC TTAAATGGAG CTTCATGAAT 
AAGCCCTCAG ATGGCAGGCC CAAGTATCTG GTGGTGAACG CGGATGAGGG GGAGCCAGGC 
ACGTGCAAGG ACCGAGAGAT CATCCGCCAC GACCCCCACA AGCTGGTGGA AGGCTGCCTA 
GTAGGGGGCC GGGCCATGGG CGCTCGTGCC GCCTACATCT ACATCCGCGG GGAGTTCTAC 
AACGAGGCCT CCAATCTGCA GGTAGCCATC CGAGAGGCCT ACGAGGCTGG TCTGATTGGC 
AAGAATGCCT GCGGCTCCGG CTACGATTTC GACGTGTTTG TGGTGCGTGG GGCCGGGGCC 
TACATCTGCG GGGAGGAGAC GGCGCTCATC GAGTCCATCG AGGGCAAACA GGGCAAGCCC 
CGCCTGAAGC CGCCTTTCCC TGCAGACGTG GGAGTGTTTG GCTGCCCTAC AACTGTGGCC 
AATGTGGAGA CTGTGGCCGT GTCCCCTACC ATCTGCCGCC GTGGGGGTGC CTGGTTTGCC 
AGCTTCGGCC GAGAGCGCAA CTCGGGCACC AAACTGTTCA ACATCTCTGG CCACGTCAAC 
AACCCCTGCA CCGTGGAGGA GGAGATGTCT GTGCCGCTGA AGGAGCTGAT TGAAAAGCAC 
GCCGGGGGTG TCACGGGTGG CTGGGACAAC CTCCTTGCTG TGATCCCTGG CGGCTCGTCC 
ACCCCGCTGA TCCCCAAGTC CGTGTGTGAG ACGGTGCTGA TGGACTTCGA CGCGCTGATC 
CAGGCGCAGA CGGGGCTGGG CACGGCTGCC GTGATCGTCA TGGACCGCTC GACGGACATT 
GTGAAAGCCA TCGCCCGCCT CATCGAGTTC TACAAGCATG AGAGCTGTGG CCAGTGTACC 
CCGTGCCGCG AGGGTGTGGA CTGGATGAAC AAGGTGATGG CCCGCTTTGT GAGGGGAGAT 
GCCCGGCCGG CAGAGATCGA CTCTCTGTGG GAGATCAGCA AGCAGATCGA GGGCCACACC 
ATCTGCGCCC TGGGCGATGG GGCTGCGTGG CCCGTGCAGG GCCTGATCCG ACACTTCCGG 
CCGGAGCTCG AGGAGCGGAT GCAGCAGTTT GCCCAGCAGC ACCAGGCCAG GCAAGCAGCT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819350.1
CDS	16..1581
Translation	MPASRQEKGGPEAHGTTSPSSLSARQGTSGLSALPRGGRAKGGAGAARGSWGAPCQGACDVLPVRRLASRSCRVLSLRAVKTAPKKTSFGSLKDEDRIFTNLYGRHDWRLKGAQSRGDWYKTKEILLKGPDWILGEVKTSGLRGRGGAGFPTGLKWSFMNKPSDGRPKYLVVNADEGEPGTCKDREIIRHDPHKLVEGCLVGGRAMGARAAYIYIRGEFYNEASNLQVAIREAYEAGLIGKNACGSGYDFDVFVVRGAGAYICGEETALIESIEGKQGKPRLKPPFPADVGVFGCPTTVANVETVAVSPTICRRGGAWFASFGRERNSGTKLFNISGHVNNPCTVEEEMSVPLKELIEKHAGGVTGGWDNLLAVIPGGSSTPLIPKSVCETVLMDFDALIQAQTGLGTAAVIVMDRSTDIVKAIARLIEFYKHESCGQCTPCREGVDWMNKVMARFVRGDARPAEIDSLWEISKQIEGHTICALGDGAAWPVQGLIRHFRPELEERMQQFAQQHQARQAAF

Target ORF information:

RefSeq Version	XM_010821048.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase core subunit V1 (NDUFV1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010821048.1

ORF Insert Sequence:

ATGCCAGCCT CGCGACAGGA GAAAGGCGGT CCGGAGGCCC ACGGGACTAC ATCTCCCAGC 
AGCCTCAGCG CCAGACAGGG CACTTCCGGC CTCTCCGCGC TCCCTCGAGG AGGCAGGGCC 
AAGGGCGGGG CGGGAGCGGC GCGGGGAAGT TGGGGCGCGC CCTGTCAGGG CGCGTGTGAC 
GTTCTTCCTG TGCGCCGCCT AGCGTCTCGG TCGTGCCGGG TTCTGAGCCT CAGAGCTGTG 
AAGACAGCGC CCAAGAAAAC CTCATTTGGC TCGCTGAAGG ACGAAGACCG GATCTTCACC 
AACCTGTATG GCCGCCATGA CTGGAGGCTT AAAGGTGCCC AGAGTCGAGG TGACTGGTAC 
AAGACGAAGG AGATTCTGCT GAAGGGGCCT GACTGGATCC TGGGTGAGGT CAAGACGTCG 
GGCTTGCGGG GCCGTGGAGG TGCTGGCTTC CCCACTGGCC TTAAATGGAG CTTCATGAAT 
AAGCCCTCAG ATGGCAGGCC CAAGTATCTG GTGGTGAACG CGGATGAGGG GGAGCCAGGC 
ACGTGCAAGG ACCGAGAGAT CATCCGCCAC GACCCCCACA AGCTGGTGGA AGGCTGCCTA 
GTAGGGGGCC GGGCCATGGG CGCTCGTGCC GCCTACATCT ACATCCGCGG GGAGTTCTAC 
AACGAGGCCT CCAATCTGCA GGTAGCCATC CGAGAGGCCT ACGAGGCTGG TCTGATTGGC 
AAGAATGCCT GCGGCTCCGG CTACGATTTC GACGTGTTTG TGGTGCGTGG GGCCGGGGCC 
TACATCTGCG GGGAGGAGAC GGCGCTCATC GAGTCCATCG AGGGCAAACA GGGCAAGCCC 
CGCCTGAAGC CGCCTTTCCC TGCAGACGTG GGAGTGTTTG GCTGCCCTAC AACTGTGGCC 
AATGTGGAGA CTGTGGCCGT GTCCCCTACC ATCTGCCGCC GTGGGGGTGC CTGGTTTGCC 
AGCTTCGGCC GAGAGCGCAA CTCGGGCACC AAACTGTTCA ACATCTCTGG CCACGTCAAC 
AACCCCTGCA CCGTGGAGGA GGAGATGTCT GTGCCGCTGA AGGAGCTGAT TGAAAAGCAC 
GCCGGGGGTG TCACGGGTGG CTGGGACAAC CTCCTTGCTG TGATCCCTGG CGGCTCGTCC 
ACCCCGCTGA TCCCCAAGTC CGTGTGTGAG ACGGTGCTGA TGGACTTCGA CGCGCTGATC 
CAGGCGCAGA CGGGGCTGGG CACGGCTGCC GTGATCGTCA TGGACCGCTC GACGGACATT 
GTGAAAGCCA TCGCCCGCCT CATCGAGTTC TACAAGCATG AGAGCTGTGG CCAGTGTACC 
CCGTGCCGCG AGGGTGTGGA CTGGATGAAC AAGGTGATGG CCCGCTTTGT GAGGGGAGAT 
GCCCGGCCGG CAGAGATCGA CTCTCTGTGG GAGATCAGCA AGCAGATCGA GGGCCACACC 
ATCTGCGCCC TGGGCGATGG GGCTGCGTGG CCCGTGCAGG GCCTGATCCG ACACTTCCGG 
CCGGAGCTCG AGGAGCGGAT GCAGCAGTTT GCCCAGCAGC ACCAGGCCAG GCAAGCAGCT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Analytical biochemistry382(2)116-21(2008 Nov)
Lemma-Gray P,Valusová E,Carroll CA,Weintraub ST,Musatov A,Robinson NC

Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme.
Biochemistry39(24)7229-35(2000 Jun)
Sazanov LA,Peak-Chew SY,Fearnley IM,Walker JE

cDNA-derived amino acid sequence of the NADH-binding 51-kDa subunit of the bovine respiratory NADH dehydrogenase reveals striking similarities to a bacterial NAD(+)-reducing hydrogenase.
Proceedings of the National Academy of Sciences of the United States of America88(10)4225-9(1991 May)
Patel SD,Aebersold R,Attardi G

Relationship between mitochondrial NADH-ubiquinone reductase and a bacterial NAD-reducing hydrogenase.
Biochemistry30(8)2166-75(1991 Feb)
Pilkington SJ,Skehel JM,Gennis RB,Walker JE