CACNA1A gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
NM_001075129.1	NP_001068597.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A
XM_024993848.1	XP_024849616.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
XM_024993850.1	XP_024849618.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
XM_024993853.1	XP_024849621.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
XM_024993858.1	XP_024849626.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
XM_024993859.1	XP_024849627.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11
XM_024993849.1	XP_024849617.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
XM_024993851.1	XP_024849619.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
XM_024993854.1	XP_024849622.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7
XM_024993856.1	XP_024849624.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X9
XM_024993855.1	XP_024849623.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
XM_024993852.1	XP_024849620.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01216	NM_001075129.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa235770	XM_024993848.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa235771	XM_024993849.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa235772	XM_024993850.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa235773	XM_024993851.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa235774	XM_024993852.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa235775	XM_024993853.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa235776	XM_024993854.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa235777	XM_024993855.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa235778	XM_024993856.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa235779	XM_024993858.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa235780	XM_024993859.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

CACNA1A ( NM_001075129.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001068597.1 Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), mRNA.

Price & Availability↑

CloneID	OBa01216
Clone ID Related Accession (Same CDS sequence)	NM_001075129.1
Accession Version	NM_001075129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ207750.1. On Oct 28, 2006 this sequence version replaced XM_613176.2. ##Evidence-Data-START## Transcript exon combination :: DQ207750.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145431, SAMN03145437 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGTTGGA 
GCTCTGCGGA GAGCCACTAT AAAGAAGAGC AAGACAGACC TGCTCAACCC CGAGGAGGCT 
GAGGATCAGC TGGCCGACAT CGCCTCTGTG GGGTCTCCCT TCGCCCGAGC CAGCATTAAA 
AGTGCCAAGC TGGAGAACTC AACTTTTTTT CACAAAAAGG AGAGGAGGAT GCGTTTCTAC 
ATCCGCCGCA TGGTCAAAAC TCAGGCCTTC TACTGGACTG TGCTCAGTCT GGTAGCCCTC 
AACACGCTGT GTGTCGCTAT CGTTCACTAC AACCAGCCTG AGTGGCTCTC CGACTTCCTC 
TACTATGCAG AATTCATTTT CTTAGGACTC TTTATGTCCG AAATGTTTAT AAAAATGTAC 
GGGCTTGGGA CGCGGCCTTA CTTCCACTCT TCCTTCAACT GTTTTGATTG TGGGGTCATC 
ATCGGGAGTA TCTTTGAGGT CATCTGGGCT GTCATAAAAC CCGGCACATC CTTTGGAATC 
AGTGTGTTAC GAGCCCTCAG GTTATTGCGT ATTTTCAAAG TCACAAAGTA CTGGGCATCT 
CTCAGGAACC TGGTCGTCTC TCTTCTCAAC TCCATGAAAT CCATCATCAG CCTGTTGTTC 
CTCCTTTTCC TATTCATCGT CGTCTTTGCC CTTCTGGGAA TGCAACTCTT TGGCGGCCAG 
TTTAATTTCG ATGAAGGGAC TCCTCCTACC AACTTCGACA CTTTTCCAGC AGCAATAATG 
ACAGTTTTTC AGATCCTGAC GGGCGAAGAC TGGAACGAGG TCATGTATGA CGGCATCAAG 
TCTCAGGGGG GCGTGCAAGG CGGCATGGTG TTCTCCATCT ACTTCATCGT GCTGACGCTC 
TTCGGGAACT ACACCCTCCT GAACGTGTTC TTGGCCATCG CGGTGGATAA TCTGGCTAAC 
GCCCAGGAGC TCACCAAGGA TGAACAAGAA GAAGAAGAGG CAGCCAACCA GAAACTTGCC 
CTACAGAAAG CCAAGGAGGT GGCAGAAGTG AGTCCTCTGT CTGCAGCCAA CATGTCCATA 
GCTGTGAAGG AGCAACAGAA GAACCAGAAG CCCGCCAAGT CGGTGTGGGA GCAGCGGACA 
AGCGAGATGA GGAAGCAGAA TCTTCTGGCC AGTCGGGAGG CACTCTACAA CGAGATGGAC 
CCAGACGAGC GCTGGAAGGC CTCCTACGCT CGCCACCTGC GGCCCGACAT GAAGACCCAC 
CTGGACCGGC CGCTGGTGGT CGACCCCCAG GAGAACCGTA ACAACAACAC CAACAAGAGC 
CGGGCGGCCG AGCCCACGGT GGACCAGCGC CTCGGGCAGC AGCGAGCCGA GGACTTCCTC 
AGGAAACAGG CCCGCTACCA CGACCGGGCC CGGGACCCCA GCAGCTCCGT GGGCCTGGAC 
ACCCGGAGGC CCTGGGCGGG CAGCCAGGAA GCCGAGCTGA GCCGGGAGGG GCCTTACGGT 
CGCGAGTCGG ACCACCACGC CCGGGAGGGC GGCCTGGAGC AGCCGGGGTT CTGGGAGGGC 
GAGGCGGAGC GGGGCAAGGC GGGCGACCCG CACCGGAGGC ACGCGCACCG GCAGGCGGGC 
AGCCGCGAGA GCCGCAGCGG GTCCCCGCGC ACCGGGGCCG ACGGAGAGCC CCGCCGGCAC 
CGGGCGCACC GCAGGCCGGG CGACGAGGCC CCGGACGACA AGGCCGATCG GAGGTCCCGG 
CACCGCGAGG GCAGCCGGCC GGCCCGGGGC GGCGAGGGCG AGAGCGAGGG CCCAGACGGC 
GGCGGCGAGC GGAGGCGGCG GCACCGGCAT GGCCCTCCCC CAGCGTACGA CGCGGACATG 
CGCAGGGAGG ACAAGGAGCG CAGGCACCGC AGGAGGAAAG AGAACCAGGG CTCCGGGGTC 
CCCGTGTCAG GCCCCAACCT GTCGACCACA CGGCCAATCC AGCAGGACCT GGGCCGCCAA 
GACCCCCCGC TGGCCGAGGA TATCGACAAC ATGAAGAACA ACAAACTGGC CACTGCGGAG 
TCAGCCAGTC CCCACGACAG CCTCAGCCAC GCCGGCCTGC CCCAGAGCCC AGCCAAGATG 
GGGAACAGCA TGGACCCTGG CCCCACACCG GCCCCCACCA CCACGGCCAC CAACCCTCAG 
AATGCCGTCA GTCGCCGGAC AGCCAACAAC CCGGGGAACC CGTCCAACCC CGGCCCCCCC 
AAGACCCCTG AGAACAGCCT TATCGTCACC AACCCCAGCA GCACCCAGGC CAACTCAGCT 
AAGACTGCCA GGAAACCTGA CCACACCACG GTGGACATCC CCCCAGCCTG CCCCCCCCCA 
CTCAACCACA CCATCATCCA AGTGAACAAA AACGCCAACC CAGACCCACT GCCAAAAAAG 
GAGGAAGAGA AGAAAGAGGA GGAGGAAGAC GACCCCGGGG AAGACGGCCC CAAGCCCATG 
CCCCCCTACA GCTCCATGTT TATCCTCTCT ACGACCAACC CCCTTCGCCG CCTGTGCCAT 
TACATCCTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCA TGGTCATCGC CATGAGCAGC 
ATCGCCCTGG CGGCCGAGGA TCCTGTTCAG CCCAACGCAC CACGAAACAA TGTGCTGCGA 
TATTTTGACT ACGTTTTTAC AGGCGTCTTT ACCTTTGAGA TGGTGATCAA GATGATCGAC 
CTGGGGCTCG TCCTGCACCA GGGTGCCTAC TTCCGTGACC TCTGGAACAT TCTCGACTTC 
ATAGTGGTCA GTGGGGCCCT GGTGGCCTTT GCCTTCACTG GCAATAGCAA AGGAAAGGAC 
ATCAACACGA TTAAATCCCT CCGAGTCCTC CGGGTGCTAC GACCTCTTAA AACCATCAAG 
CGGTTGCCAA AGCTCAAGGC CGTGTTTGAC TGCGTGGTGA ACTCGCTCAA GAACGTCTTC 
AACATCCTCA TCGTCTACAT GCTGTTCATG TTCATCTTCG CCGTGGTGGC CGTGCAGCTC 
TTCAAGGGCA AGTTTTTCCA CTGCACGGAT GAGTCCAAGG AGTTTGAGAA AGATTGTCGC 
GGCAAGTACC TCCTCTATGA GAAGAATGAG GTAAAGGCGA GGGATCGAGA GTGGAAGAAG 
TATGAGTTCC ATTATGACAA TGTGCTGTGG GCGCTGCTGA CCCTCTTCAC CGTGTCCACG 
GGAGAAGGCT GGCCACAGGT CCTCAAGCAC TCGGTGGACG CCACCTTTGA GAACCAGGGC 
CCCAGCCCCG GGTACCGCAT GGAGATGTCC ATCTTCTACG TCGTCTACTT TGTGGTGTTC 
CCCTTCTTCT TTGTCAACAT CTTTGTGGCC TTGATCATCA TCACCTTCCA GGAGCAGGGG 
GACAAGATGA TGGAGGAGTA CAGCCTGGAG AAAAATGAGA GGGCGTGCAT CGACTTCGCC 
ATCAGCGCCA AACCCCTGAC CCGGCACATG CCGCAGAACA AGCAGAGTTT CCAGTACCGC 
ATGTGGCAGT TCGTGGTGTC CCCGCCTTTC GAGTACACCA TCATGGCCAT GATCGCCCTC 
AACACCATCG TGCTCATGAT GAAGTTCTAC GGGGCCTCTG TTGCTTATGA GAATGCTCTG 
AGAGTATTCA ACATCGTCTT CACCTCCCTC TTCTCACTGG AATGTGTGCT GAAAGTCATG 
GCTTTTGGGA TTCTGAATTA TTTTCGCGAT GCCTGGAACA TCTTCGACTT TGTGACTGTT 
CTGGGCAGCA TCACCGACAT CCTCGTGACC GAGTTTGGGA ATAACTTCAT CAACTTGAGC 
TTCCTCCGCC TCTTCCGAGC TGCCCGGCTC ATCAAACTCC TCCGTCAGGG TTACACCATC 
CGAATTCTTC TCTGGACCTT TGTGCAGTCC TTCAAGGCCC TGCCCTATGT CTGTCTACTG 
ATTGCCATGC TTTTTTTCAT CTACGCCATC ATTGGGATGC AGGTGTTTGG CAACATTGGC 
ATCGATGTGG AGGACGAGGA CAGTGATGAA GATGAATTCC AAATCACTGA GCACAATAAC 
TTCCGCACCT TCTTCCAGGC CCTCATGCTT CTCTTCCGGA GTGCCACTGG GGAGGCTTGG 
CACAACATCA TGCTTTCCTG CCTCAGCGGG AAACCCTGTG ATAAGAACTC TGGCATCCTG 
ACTCACGAAT GCGGCAATGA ATTTGCTTAT TTTTACTTCG TTTCCTTCAT CTTCCTCTGC 
TCGTTTCTGA TGCTGAATCT TTTTGTTGCC GTCATCATGG ACAACTTTGA GTACCTCACC 
CGAGATTCCT CCATCCTGGG TCCCCACCAC CTGGATGAGT ACGTGCGTGT CTGGGCCGAG 
TATGACCCTG CAGCGTGCGG TCGGATTCAT TATAAGGATA TGTACAGTTT ATTGCGTGTA 
ATATCTCCCC CTCTCGGCTT AGGCAAGAAA TGTCCTCATA GGGTTGCTTG CAAGAGGCTC 
CTGCGGATGG ATCTGCCCGT CGCTGATGAC AACACCGTCC ACTTCAACTC TACCCTCATG 
GCTCTGATCC GCACGGCCCT GGACATCAAA ATTGCCAAAG GTGGAGCTGA CAAACAACAA 
ATGGATGCTG AGTTGCGGAA GGAGATGATG GCCATCTGGC CCAATCTGTC CCAGAAGACG 
CTGGACCTGC TGGTCACCCC CCACAAGTCC ACGGACCTGA CAGTGGGGAA GATCTATGCG 
GCCATGATGA TCATGGAGTA TTACCGGCAG AGCAAGGCCA AGAAGCTTCA GGCCATGCGC 
GAGGAACAGA ACCGGACACC TCTCATGTTC CAGCGCATGG AGCCCCCATC GCCAACACAG 
GAGGGGGGGC CTGGCCAGAA CGCCCTCCCC TCCACCCAGC TGGACCCAGG AGGAGGCTTG 
ATGGCTCACG AAAGTGGCAT GAAAGAGAGC CCATCCTGGG TGACCCAGCG GGCCCAGGAG 
ATGTTCCAGA AGACGGGCAC ATGGAGCCCA GAGCGGGGCC CCCCCACTGA TATGCCTAAT 
AGCCAACCCA ACTCTCAGTC TGTGGAGATG CGAGAGATGG GCAGAGACGG CTACTCTGAC 
AGTGAGCACT ACTTCCCCGT TGAAGGCCAG GCCCGGGCCG CCTCCATGCC CCGCCTGCCT 
GCCGAGAACC AGAGGAGAAG GGGCCGGCCA CGTGGGAATA ACCTCAGTAC CATCTCAGAC 
ACCAGCCCCA TGAAGCGCTC GGCCTCCGTG CTGGGCCCCA AGGCCCGGCG CCTGGACGAC 
TACTCCCTGG AGAGGGTGCC GCCCGAGGAG AACCAGCGGC ACCACCAGAG GCGCAGAGAC 
CGTGGCCACC GCACCTCCGA GCGCTCCTTG GGCCGCTACA CGGACGTGGA CACAGGCTTG 
GGGACAGACC TGAGCATGAC CACCCAATCT GGGGACCTGC CATCAAAGGA AAGAGACCAG 
GAGCGGGGCC GGCCCAAGGA CCGGAAGCAT CGGCAACACC ACCACCACCA CCACCACCAC 
CACCATCCCC CGCCCCCTGA CAAGGAGCGC TACACCCAGG AGCGGCCTGA GCACGGCCGG 
CCCCGGGCCC GTGACCAGCG CTGGTCCCGC TCACCCAGTG AGGGCCGGGA GCACATGGCC 
CACCGTCAGG GCAGTAGTTC CGTAAGTGGA AGCCCAGCCC CCTCAACATC TGGTACCAGC 
ACTCCGCGGC GGGGACGCCG CCAGCTCCCC CAGACCCCCT CCACTCCCCG GCCGCACGTG 
TCCTATTCCC CCGTGATCCG TAAGGCCGGC GGCTCGGGTC CCCAGCAGCA GCAGCAGCAG 
CCCGCGGCGC GGCCGGGCCG AACGGCCCCC AGCGGCCCGC GGAGGTACCC GGGCCCCGTG 
GCCGAGCCCC TGGCCGGGGA GCGGCCTCCC ACGGGAGGCC ACGGAAGCAG CCGCTCGCCC 
GCGATGGAGC GGCGGATCCC GGGCCCGGCC CGGAGCGAGT CCCCCAGGGC GCCGTGTCGC 
CACGGCGGGG CGCGATGGCC GGCGTCCGGC GCGCACGCGG CCGAGGGCCC CCCGGGCCCC 
CGGCACCACC ACGGTTACTA CCGGGGCTCC GACTACGACG AGGCCGATGG CCCGGGCGGC 
GGCGGCGGGG AGGAGGAGGA GCCCCGGGCC GCCGCCTACG ACGCGCCGCC CCCCGCGCGC 
CACGGGTGCT CGCCCAGGAC TCCCCGGGCC GCGGGCCCGG CCTGCGCCTC GCCTTCCAGG 
CACGGCCGGC GACTCCCGAA CGGCTACTAC CCGGCCCACG GACTGGCCAG GCCCCGCGGG 
CCGGGCTCCC GGAAAGGCCT GCACGAAGCC TACAGCGAGA GCGACGACGA TTGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001068597.1
CDS	32..7531
Translation	MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRHPFDVGALRRATIKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSSSVGLDTRRPWAGSQEAELSREGPYGRESDHHAREGGLEQPGFWEGEAERGKAGDPHRRHAHRQAGSRESRSGSPRTGADGEPRRHRAHRRPGDEAPDDKADRRSRHREGSRPARGGEGESEGPDGGGERRRRHRHGPPPAYDADMRREDKERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESASPHDSLSHAGLPQSPAKMGNSMDPGPTPAPTTTATNPQNAVSRRTANNPGNPSNPGPPKTPENSLIVTNPSSTQANSAKTARKPDHTTVDIPPACPPPLNHTIIQVNKNANPDPLPKKEEEKKEEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTHECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPGQNALPSTQLDPGGGLMAHESGMKESPSWVTQRAQEMFQKTGTWSPERGPPTDMPNSQPNSQSVEMREMGRDGYSDSEHYFPVEGQARAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRPKDRKHRQHHHHHHHHHHPPPPDKERYTQERPEHGRPRARDQRWSRSPSEGREHMAHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPSTPRPHVSYSPVIRKAGGSGPQQQQQQPAARPGRTAPSGPRRYPGPVAEPLAGERPPTGGHGSSRSPAMERRIPGPARSESPRAPCRHGGARWPASGAHAAEGPPGPRHHHGYYRGSDYDEADGPGGGGGEEEEPRAAAYDAPPPARHGCSPRTPRAAGPACASPSRHGRRLPNGYYPAHGLARPRGPGSRKGLHEAYSESDDDWC

Target ORF information:

RefSeq Version	NM_001075129.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075129.1

ORF Insert Sequence:

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGTTGGA 
GCTCTGCGGA GAGCCACTAT AAAGAAGAGC AAGACAGACC TGCTCAACCC CGAGGAGGCT 
GAGGATCAGC TGGCCGACAT CGCCTCTGTG GGGTCTCCCT TCGCCCGAGC CAGCATTAAA 
AGTGCCAAGC TGGAGAACTC AACTTTTTTT CACAAAAAGG AGAGGAGGAT GCGTTTCTAC 
ATCCGCCGCA TGGTCAAAAC TCAGGCCTTC TACTGGACTG TGCTCAGTCT GGTAGCCCTC 
AACACGCTGT GTGTCGCTAT CGTTCACTAC AACCAGCCTG AGTGGCTCTC CGACTTCCTC 
TACTATGCAG AATTCATTTT CTTAGGACTC TTTATGTCCG AAATGTTTAT AAAAATGTAC 
GGGCTTGGGA CGCGGCCTTA CTTCCACTCT TCCTTCAACT GTTTTGATTG TGGGGTCATC 
ATCGGGAGTA TCTTTGAGGT CATCTGGGCT GTCATAAAAC CCGGCACATC CTTTGGAATC 
AGTGTGTTAC GAGCCCTCAG GTTATTGCGT ATTTTCAAAG TCACAAAGTA CTGGGCATCT 
CTCAGGAACC TGGTCGTCTC TCTTCTCAAC TCCATGAAAT CCATCATCAG CCTGTTGTTC 
CTCCTTTTCC TATTCATCGT CGTCTTTGCC CTTCTGGGAA TGCAACTCTT TGGCGGCCAG 
TTTAATTTCG ATGAAGGGAC TCCTCCTACC AACTTCGACA CTTTTCCAGC AGCAATAATG 
ACAGTTTTTC AGATCCTGAC GGGCGAAGAC TGGAACGAGG TCATGTATGA CGGCATCAAG 
TCTCAGGGGG GCGTGCAAGG CGGCATGGTG TTCTCCATCT ACTTCATCGT GCTGACGCTC 
TTCGGGAACT ACACCCTCCT GAACGTGTTC TTGGCCATCG CGGTGGATAA TCTGGCTAAC 
GCCCAGGAGC TCACCAAGGA TGAACAAGAA GAAGAAGAGG CAGCCAACCA GAAACTTGCC 
CTACAGAAAG CCAAGGAGGT GGCAGAAGTG AGTCCTCTGT CTGCAGCCAA CATGTCCATA 
GCTGTGAAGG AGCAACAGAA GAACCAGAAG CCCGCCAAGT CGGTGTGGGA GCAGCGGACA 
AGCGAGATGA GGAAGCAGAA TCTTCTGGCC AGTCGGGAGG CACTCTACAA CGAGATGGAC 
CCAGACGAGC GCTGGAAGGC CTCCTACGCT CGCCACCTGC GGCCCGACAT GAAGACCCAC 
CTGGACCGGC CGCTGGTGGT CGACCCCCAG GAGAACCGTA ACAACAACAC CAACAAGAGC 
CGGGCGGCCG AGCCCACGGT GGACCAGCGC CTCGGGCAGC AGCGAGCCGA GGACTTCCTC 
AGGAAACAGG CCCGCTACCA CGACCGGGCC CGGGACCCCA GCAGCTCCGT GGGCCTGGAC 
ACCCGGAGGC CCTGGGCGGG CAGCCAGGAA GCCGAGCTGA GCCGGGAGGG GCCTTACGGT 
CGCGAGTCGG ACCACCACGC CCGGGAGGGC GGCCTGGAGC AGCCGGGGTT CTGGGAGGGC 
GAGGCGGAGC GGGGCAAGGC GGGCGACCCG CACCGGAGGC ACGCGCACCG GCAGGCGGGC 
AGCCGCGAGA GCCGCAGCGG GTCCCCGCGC ACCGGGGCCG ACGGAGAGCC CCGCCGGCAC 
CGGGCGCACC GCAGGCCGGG CGACGAGGCC CCGGACGACA AGGCCGATCG GAGGTCCCGG 
CACCGCGAGG GCAGCCGGCC GGCCCGGGGC GGCGAGGGCG AGAGCGAGGG CCCAGACGGC 
GGCGGCGAGC GGAGGCGGCG GCACCGGCAT GGCCCTCCCC CAGCGTACGA CGCGGACATG 
CGCAGGGAGG ACAAGGAGCG CAGGCACCGC AGGAGGAAAG AGAACCAGGG CTCCGGGGTC 
CCCGTGTCAG GCCCCAACCT GTCGACCACA CGGCCAATCC AGCAGGACCT GGGCCGCCAA 
GACCCCCCGC TGGCCGAGGA TATCGACAAC ATGAAGAACA ACAAACTGGC CACTGCGGAG 
TCAGCCAGTC CCCACGACAG CCTCAGCCAC GCCGGCCTGC CCCAGAGCCC AGCCAAGATG 
GGGAACAGCA TGGACCCTGG CCCCACACCG GCCCCCACCA CCACGGCCAC CAACCCTCAG 
AATGCCGTCA GTCGCCGGAC AGCCAACAAC CCGGGGAACC CGTCCAACCC CGGCCCCCCC 
AAGACCCCTG AGAACAGCCT TATCGTCACC AACCCCAGCA GCACCCAGGC CAACTCAGCT 
AAGACTGCCA GGAAACCTGA CCACACCACG GTGGACATCC CCCCAGCCTG CCCCCCCCCA 
CTCAACCACA CCATCATCCA AGTGAACAAA AACGCCAACC CAGACCCACT GCCAAAAAAG 
GAGGAAGAGA AGAAAGAGGA GGAGGAAGAC GACCCCGGGG AAGACGGCCC CAAGCCCATG 
CCCCCCTACA GCTCCATGTT TATCCTCTCT ACGACCAACC CCCTTCGCCG CCTGTGCCAT 
TACATCCTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCA TGGTCATCGC CATGAGCAGC 
ATCGCCCTGG CGGCCGAGGA TCCTGTTCAG CCCAACGCAC CACGAAACAA TGTGCTGCGA 
TATTTTGACT ACGTTTTTAC AGGCGTCTTT ACCTTTGAGA TGGTGATCAA GATGATCGAC 
CTGGGGCTCG TCCTGCACCA GGGTGCCTAC TTCCGTGACC TCTGGAACAT TCTCGACTTC 
ATAGTGGTCA GTGGGGCCCT GGTGGCCTTT GCCTTCACTG GCAATAGCAA AGGAAAGGAC 
ATCAACACGA TTAAATCCCT CCGAGTCCTC CGGGTGCTAC GACCTCTTAA AACCATCAAG 
CGGTTGCCAA AGCTCAAGGC CGTGTTTGAC TGCGTGGTGA ACTCGCTCAA GAACGTCTTC 
AACATCCTCA TCGTCTACAT GCTGTTCATG TTCATCTTCG CCGTGGTGGC CGTGCAGCTC 
TTCAAGGGCA AGTTTTTCCA CTGCACGGAT GAGTCCAAGG AGTTTGAGAA AGATTGTCGC 
GGCAAGTACC TCCTCTATGA GAAGAATGAG GTAAAGGCGA GGGATCGAGA GTGGAAGAAG 
TATGAGTTCC ATTATGACAA TGTGCTGTGG GCGCTGCTGA CCCTCTTCAC CGTGTCCACG 
GGAGAAGGCT GGCCACAGGT CCTCAAGCAC TCGGTGGACG CCACCTTTGA GAACCAGGGC 
CCCAGCCCCG GGTACCGCAT GGAGATGTCC ATCTTCTACG TCGTCTACTT TGTGGTGTTC 
CCCTTCTTCT TTGTCAACAT CTTTGTGGCC TTGATCATCA TCACCTTCCA GGAGCAGGGG 
GACAAGATGA TGGAGGAGTA CAGCCTGGAG AAAAATGAGA GGGCGTGCAT CGACTTCGCC 
ATCAGCGCCA AACCCCTGAC CCGGCACATG CCGCAGAACA AGCAGAGTTT CCAGTACCGC 
ATGTGGCAGT TCGTGGTGTC CCCGCCTTTC GAGTACACCA TCATGGCCAT GATCGCCCTC 
AACACCATCG TGCTCATGAT GAAGTTCTAC GGGGCCTCTG TTGCTTATGA GAATGCTCTG 
AGAGTATTCA ACATCGTCTT CACCTCCCTC TTCTCACTGG AATGTGTGCT GAAAGTCATG 
GCTTTTGGGA TTCTGAATTA TTTTCGCGAT GCCTGGAACA TCTTCGACTT TGTGACTGTT 
CTGGGCAGCA TCACCGACAT CCTCGTGACC GAGTTTGGGA ATAACTTCAT CAACTTGAGC 
TTCCTCCGCC TCTTCCGAGC TGCCCGGCTC ATCAAACTCC TCCGTCAGGG TTACACCATC 
CGAATTCTTC TCTGGACCTT TGTGCAGTCC TTCAAGGCCC TGCCCTATGT CTGTCTACTG 
ATTGCCATGC TTTTTTTCAT CTACGCCATC ATTGGGATGC AGGTGTTTGG CAACATTGGC 
ATCGATGTGG AGGACGAGGA CAGTGATGAA GATGAATTCC AAATCACTGA GCACAATAAC 
TTCCGCACCT TCTTCCAGGC CCTCATGCTT CTCTTCCGGA GTGCCACTGG GGAGGCTTGG 
CACAACATCA TGCTTTCCTG CCTCAGCGGG AAACCCTGTG ATAAGAACTC TGGCATCCTG 
ACTCACGAAT GCGGCAATGA ATTTGCTTAT TTTTACTTCG TTTCCTTCAT CTTCCTCTGC 
TCGTTTCTGA TGCTGAATCT TTTTGTTGCC GTCATCATGG ACAACTTTGA GTACCTCACC 
CGAGATTCCT CCATCCTGGG TCCCCACCAC CTGGATGAGT ACGTGCGTGT CTGGGCCGAG 
TATGACCCTG CAGCGTGCGG TCGGATTCAT TATAAGGATA TGTACAGTTT ATTGCGTGTA 
ATATCTCCCC CTCTCGGCTT AGGCAAGAAA TGTCCTCATA GGGTTGCTTG CAAGAGGCTC 
CTGCGGATGG ATCTGCCCGT CGCTGATGAC AACACCGTCC ACTTCAACTC TACCCTCATG 
GCTCTGATCC GCACGGCCCT GGACATCAAA ATTGCCAAAG GTGGAGCTGA CAAACAACAA 
ATGGATGCTG AGTTGCGGAA GGAGATGATG GCCATCTGGC CCAATCTGTC CCAGAAGACG 
CTGGACCTGC TGGTCACCCC CCACAAGTCC ACGGACCTGA CAGTGGGGAA GATCTATGCG 
GCCATGATGA TCATGGAGTA TTACCGGCAG AGCAAGGCCA AGAAGCTTCA GGCCATGCGC 
GAGGAACAGA ACCGGACACC TCTCATGTTC CAGCGCATGG AGCCCCCATC GCCAACACAG 
GAGGGGGGGC CTGGCCAGAA CGCCCTCCCC TCCACCCAGC TGGACCCAGG AGGAGGCTTG 
ATGGCTCACG AAAGTGGCAT GAAAGAGAGC CCATCCTGGG TGACCCAGCG GGCCCAGGAG 
ATGTTCCAGA AGACGGGCAC ATGGAGCCCA GAGCGGGGCC CCCCCACTGA TATGCCTAAT 
AGCCAACCCA ACTCTCAGTC TGTGGAGATG CGAGAGATGG GCAGAGACGG CTACTCTGAC 
AGTGAGCACT ACTTCCCCGT TGAAGGCCAG GCCCGGGCCG CCTCCATGCC CCGCCTGCCT 
GCCGAGAACC AGAGGAGAAG GGGCCGGCCA CGTGGGAATA ACCTCAGTAC CATCTCAGAC 
ACCAGCCCCA TGAAGCGCTC GGCCTCCGTG CTGGGCCCCA AGGCCCGGCG CCTGGACGAC 
TACTCCCTGG AGAGGGTGCC GCCCGAGGAG AACCAGCGGC ACCACCAGAG GCGCAGAGAC 
CGTGGCCACC GCACCTCCGA GCGCTCCTTG GGCCGCTACA CGGACGTGGA CACAGGCTTG 
GGGACAGACC TGAGCATGAC CACCCAATCT GGGGACCTGC CATCAAAGGA AAGAGACCAG 
GAGCGGGGCC GGCCCAAGGA CCGGAAGCAT CGGCAACACC ACCACCACCA CCACCACCAC 
CACCATCCCC CGCCCCCTGA CAAGGAGCGC TACACCCAGG AGCGGCCTGA GCACGGCCGG 
CCCCGGGCCC GTGACCAGCG CTGGTCCCGC TCACCCAGTG AGGGCCGGGA GCACATGGCC 
CACCGTCAGG GCAGTAGTTC CGTAAGTGGA AGCCCAGCCC CCTCAACATC TGGTACCAGC 
ACTCCGCGGC GGGGACGCCG CCAGCTCCCC CAGACCCCCT CCACTCCCCG GCCGCACGTG 
TCCTATTCCC CCGTGATCCG TAAGGCCGGC GGCTCGGGTC CCCAGCAGCA GCAGCAGCAG 
CCCGCGGCGC GGCCGGGCCG AACGGCCCCC AGCGGCCCGC GGAGGTACCC GGGCCCCGTG 
GCCGAGCCCC TGGCCGGGGA GCGGCCTCCC ACGGGAGGCC ACGGAAGCAG CCGCTCGCCC 
GCGATGGAGC GGCGGATCCC GGGCCCGGCC CGGAGCGAGT CCCCCAGGGC GCCGTGTCGC 
CACGGCGGGG CGCGATGGCC GGCGTCCGGC GCGCACGCGG CCGAGGGCCC CCCGGGCCCC 
CGGCACCACC ACGGTTACTA CCGGGGCTCC GACTACGACG AGGCCGATGG CCCGGGCGGC 
GGCGGCGGGG AGGAGGAGGA GCCCCGGGCC GCCGCCTACG ACGCGCCGCC CCCCGCGCGC 
CACGGGTGCT CGCCCAGGAC TCCCCGGGCC GCGGGCCCGG CCTGCGCCTC GCCTTCCAGG 
CACGGCCGGC GACTCCCGAA CGGCTACTAC CCGGCCCACG GACTGGCCAG GCCCCGCGGG 
CCGGGCTCCC GGAAAGGCCT GCACGAAGCC TACAGCGAGA GCGACGACGA TTGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1A ( XM_024993848.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849616.1 Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X1, mRNA.

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CloneID	OBa235770
Clone ID Related Accession (Same CDS sequence)	XM_024993848.1
Accession Version	XM_024993848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA 
GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA 
ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG 
AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC 
GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA 
GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG 
TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC 
GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG 
AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT 
GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC 
GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC 
CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG 
ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG 
AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG 
AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT 
GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC 
AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT 
TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC 
TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC 
AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG 
CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG 
GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT 
GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC 
TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG 
TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC 
TGGGCCGAGT ATGACCCTGC AGCGTGCGGT CGGATTCATT ATAAGGATAT GTACAGTTTA 
TTGCGCGTAA TATCTCCCCC TCTCGGCTTA GGCAAGAAAT GTCCTCATAG GGTTGCTTGC 
AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT 
ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC 
AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC 
CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG 
ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG 
GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG 
CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA 
GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG 
GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT 
ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC 
TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC 
CGCCTGCCTG CCGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC 
ATCTCAGACA CCAGCCCCAT GAAGCGCTCG GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC 
CTGGACGACT ACTCCCTGGA GAGGGTGCCG CCCGAGGAGA ACCAGCGGCA CCACCAGAGG 
CGCAGAGACC GTGGCCACCG CACCTCCGAG CGCTCCTTGG GCCGCTACAC GGACGTGGAC 
ACAGGCTTGG GGACAGACTT GAGCATGACC ACCCAGTCTG GGGACCTGCC ATCAAAGGAA 
AGAGACCAGG AGCGGGGCCG GCCCAAGGAC CGGAAGCATC GGCAACACCA CCACCACCAC 
CACCACCACC ATCCCCCGCC CCCTGACAAG GAGCGCTACA CCCAGGAGCG GCCTGAGCAC 
GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC 
ATGGCCCACC GTCAGGGCAG TAGTTCCGTA AGTGGAAGCC CAGCCCCCTC AACATCTGGT 
ACCAGCACTC CGCGGCGGGG ACGCCGCCAG CTCCCCCAGA CCCCCTCCAC TCCCCGGCCG 
CACGTGTCCT ATTCCCCCGT GATCCGTAAG GCCGGCGGCT CGGGTCCCCA GCAGCAGCAG 
CAGCCCGCGG CGCGGCCGGG CCGAACGGCC CCCAGCGGCC CGCGGAGGTA CCCGGGCCCC 
GTGGCCGAGC CCCTGGCCGG GGAGCGGCCT CCCACGGGAG GCCACGGAAG CAGCCGCTCG 
CCCGCGATGG AGCGGCGGAT CCCGGGCCCG GCCCGGAGCG AGTCCCCCAG GGCGCCGTGT 
CGCCACGGCG GGGCGCGATG GCCGGCGTCC GGCGCGCACG CGGCCGAGGG CCCCCCGGGC 
CCCCGGCACC ACCACGGTTA CTACCGGGGC TCCGACTACG ACGAGCCAGA CGGCCCGGGC 
GGCGGCGGCG GGGAGGAGGA GGAGCCCCGG GCCGCCGCCT ACGACGCGCC GCCCCCCGCG 
CGCCACGGGT GCTCGCCCAG GACTCCCCGG GCCGCGGGCC CGGCCTGCGC CTCGCCTTCC 
AGGCACGGCC GGCGACTCCC GAACGGCTAC TACCCGGCCC ACGGACTGGC CAGGCCCCGC 
GGGCCGGGCT CCCGGAAAGG CCTGCACGAA GCCTACAGCG AGACCGACGA CGATGACTGG 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849616.1
CDS	230..7735
Translation	MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRHPFDGALRRATIKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSSSVGLDTRRPWAGSQEAELSREGPYGRESDHHAREGGLEQPGFWEGEAERGKAGDPHRRHAHRQAGSRESRSGSPRTGADGEPRRHRAHRRPGDEAPDDKADRRSRHREGSRPARGGEGESEGPDGGGERRRRHRHGPPPAYDADMRREDKERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESASPHDSLSHAGLPQSPAKMGNSMDPGPTPAPTTTATNPQNAVSRRTANNPGNPSNPGPPKTPENSLIVTNPSSTQANSAKTARKPDHTTVDIPPACPPPLNHTIIQVNKNANPDPLPKKEEEKKEEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTHECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPGQNALPSTQLDPGGGLMAHESGMKESPSWVTQRAQEMFQKTGTWSPERGPPTDMPNSQPNSQSVEMREMGRDGYSDSEHYFPVEGQARAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRPKDRKHRQHHHHHHHHHPPPPDKERYTQERPEHGRPRARDQRWSRSPSEGREHMAHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPSTPRPHVSYSPVIRKAGGSGPQQQQQPAARPGRTAPSGPRRYPGPVAEPLAGERPPTGGHGSSRSPAMERRIPGPARSESPRAPCRHGGARWPASGAHAAEGPPGPRHHHGYYRGSDYDEPDGPGGGGGEEEEPRAAAYDAPPPARHGCSPRTPRAAGPACASPSRHGRRLPNGYYPAHGLARPRGPGSRKGLHEAYSETDDDDWC

Target ORF information:

RefSeq Version	XM_024993848.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993848.1

ORF Insert Sequence:

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA 
GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA 
ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG 
AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC 
GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA 
GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG 
TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC 
GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG 
AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT 
GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC 
GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC 
CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG 
ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG 
AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG 
AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT 
GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC 
AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT 
TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC 
TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC 
AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG 
CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG 
GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT 
GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC 
TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG 
TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC 
TGGGCCGAGT ATGACCCTGC AGCGTGCGGT CGGATTCATT ATAAGGATAT GTACAGTTTA 
TTGCGCGTAA TATCTCCCCC TCTCGGCTTA GGCAAGAAAT GTCCTCATAG GGTTGCTTGC 
AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT 
ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC 
AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC 
CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG 
ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG 
GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG 
CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA 
GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG 
GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT 
ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC 
TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC 
CGCCTGCCTG CCGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC 
ATCTCAGACA CCAGCCCCAT GAAGCGCTCG GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC 
CTGGACGACT ACTCCCTGGA GAGGGTGCCG CCCGAGGAGA ACCAGCGGCA CCACCAGAGG 
CGCAGAGACC GTGGCCACCG CACCTCCGAG CGCTCCTTGG GCCGCTACAC GGACGTGGAC 
ACAGGCTTGG GGACAGACTT GAGCATGACC ACCCAGTCTG GGGACCTGCC ATCAAAGGAA 
AGAGACCAGG AGCGGGGCCG GCCCAAGGAC CGGAAGCATC GGCAACACCA CCACCACCAC 
CACCACCACC ATCCCCCGCC CCCTGACAAG GAGCGCTACA CCCAGGAGCG GCCTGAGCAC 
GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC 
ATGGCCCACC GTCAGGGCAG TAGTTCCGTA AGTGGAAGCC CAGCCCCCTC AACATCTGGT 
ACCAGCACTC CGCGGCGGGG ACGCCGCCAG CTCCCCCAGA CCCCCTCCAC TCCCCGGCCG 
CACGTGTCCT ATTCCCCCGT GATCCGTAAG GCCGGCGGCT CGGGTCCCCA GCAGCAGCAG 
CAGCCCGCGG CGCGGCCGGG CCGAACGGCC CCCAGCGGCC CGCGGAGGTA CCCGGGCCCC 
GTGGCCGAGC CCCTGGCCGG GGAGCGGCCT CCCACGGGAG GCCACGGAAG CAGCCGCTCG 
CCCGCGATGG AGCGGCGGAT CCCGGGCCCG GCCCGGAGCG AGTCCCCCAG GGCGCCGTGT 
CGCCACGGCG GGGCGCGATG GCCGGCGTCC GGCGCGCACG CGGCCGAGGG CCCCCCGGGC 
CCCCGGCACC ACCACGGTTA CTACCGGGGC TCCGACTACG ACGAGCCAGA CGGCCCGGGC 
GGCGGCGGCG GGGAGGAGGA GGAGCCCCGG GCCGCCGCCT ACGACGCGCC GCCCCCCGCG 
CGCCACGGGT GCTCGCCCAG GACTCCCCGG GCCGCGGGCC CGGCCTGCGC CTCGCCTTCC 
AGGCACGGCC GGCGACTCCC GAACGGCTAC TACCCGGCCC ACGGACTGGC CAGGCCCCGC 
GGGCCGGGCT CCCGGAAAGG CCTGCACGAA GCCTACAGCG AGACCGACGA CGATGACTGG 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1A ( XM_024993849.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849617.1 Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X2, mRNA.

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CloneID	OBa235771
Clone ID Related Accession (Same CDS sequence)	XM_024993849.1
Accession Version	XM_024993849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA 
GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA 
ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG 
AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC 
GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA 
GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG 
TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC 
GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG 
AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT 
GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC 
GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC 
CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG 
ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG 
AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG 
AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT 
GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC 
AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT 
TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC 
TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC 
AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG 
CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG 
GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT 
GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC 
TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG 
TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC 
TGGGCCGAGT ATGACCCTGC AGCGTGGGGC CGCATGCCTT ACCCGGACAT GTATCAGATG 
CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC 
AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT 
ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC 
AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC 
CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG 
ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG 
GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG 
CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA 
GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG 
GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT 
ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC 
TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC 
CGCCTGCCTG CCGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC 
ATCTCAGACA CCAGCCCCAT GAAGCGCTCG GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC 
CTGGACGACT ACTCCCTGGA GAGGGTGCCG CCCGAGGAGA ACCAGCGGCA CCACCAGAGG 
CGCAGAGACC GTGGCCACCG CACCTCCGAG CGCTCCTTGG GCCGCTACAC GGACGTGGAC 
ACAGGCTTGG GGACAGACTT GAGCATGACC ACCCAGTCTG GGGACCTGCC ATCAAAGGAA 
AGAGACCAGG AGCGGGGCCG GCCCAAGGAC CGGAAGCATC GGCAACACCA CCACCACCAC 
CACCACCACC ATCCCCCGCC CCCTGACAAG GAGCGCTACA CCCAGGAGCG GCCTGAGCAC 
GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC 
ATGGCCCACC GTCAGGGCAG TAGTTCCGTA AGTGGAAGCC CAGCCCCCTC AACATCTGGT 
ACCAGCACTC CGCGGCGGGG ACGCCGCCAG CTCCCCCAGA CCCCCTCCAC TCCCCGGCCG 
CACGTGTCCT ATTCCCCCGT GATCCGTAAG GCCGGCGGCT CGGGTCCCCA GCAGCAGCAG 
CAGCCCGCGG CGCGGCCGGG CCGAACGGCC CCCAGCGGCC CGCGGAGGTA CCCGGGCCCC 
GTGGCCGAGC CCCTGGCCGG GGAGCGGCCT CCCACGGGAG GCCACGGAAG CAGCCGCTCG 
CCCGCGATGG AGCGGCGGAT CCCGGGCCCG GCCCGGAGCG AGTCCCCCAG GGCGCCGTGT 
CGCCACGGCG GGGCGCGATG GCCGGCGTCC GGCGCGCACG CGGCCGAGGG CCCCCCGGGC 
CCCCGGCACC ACCACGGTTA CTACCGGGGC TCCGACTACG ACGAGCCAGA CGGCCCGGGC 
GGCGGCGGCG GGGAGGAGGA GGAGCCCCGG GCCGCCGCCT ACGACGCGCC GCCCCCCGCG 
CGCCACGGGT GCTCGCCCAG GACTCCCCGG GCCGCGGGCC CGGCCTGCGC CTCGCCTTCC 
AGGCACGGCC GGCGACTCCC GAACGGCTAC TACCCGGCCC ACGGACTGGC CAGGCCCCGC 
GGGCCGGGCT CCCGGAAAGG CCTGCACGAA GCCTACAGCG AGACCGACGA CGATGACTGG 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849617.1
CDS	230..7735
Translation	MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRHPFDGALRRATIKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSSSVGLDTRRPWAGSQEAELSREGPYGRESDHHAREGGLEQPGFWEGEAERGKAGDPHRRHAHRQAGSRESRSGSPRTGADGEPRRHRAHRRPGDEAPDDKADRRSRHREGSRPARGGEGESEGPDGGGERRRRHRHGPPPAYDADMRREDKERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESASPHDSLSHAGLPQSPAKMGNSMDPGPTPAPTTTATNPQNAVSRRTANNPGNPSNPGPPKTPENSLIVTNPSSTQANSAKTARKPDHTTVDIPPACPPPLNHTIIQVNKNANPDPLPKKEEEKKEEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTHECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMPYPDMYQMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPGQNALPSTQLDPGGGLMAHESGMKESPSWVTQRAQEMFQKTGTWSPERGPPTDMPNSQPNSQSVEMREMGRDGYSDSEHYFPVEGQARAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRPKDRKHRQHHHHHHHHHPPPPDKERYTQERPEHGRPRARDQRWSRSPSEGREHMAHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPSTPRPHVSYSPVIRKAGGSGPQQQQQPAARPGRTAPSGPRRYPGPVAEPLAGERPPTGGHGSSRSPAMERRIPGPARSESPRAPCRHGGARWPASGAHAAEGPPGPRHHHGYYRGSDYDEPDGPGGGGGEEEEPRAAAYDAPPPARHGCSPRTPRAAGPACASPSRHGRRLPNGYYPAHGLARPRGPGSRKGLHEAYSETDDDDWC

Target ORF information:

RefSeq Version	XM_024993849.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993849.1

ORF Insert Sequence:

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA 
GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA 
ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG 
AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC 
GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA 
GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG 
TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC 
GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG 
AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT 
GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC 
GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC 
CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG 
ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG 
AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG 
AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT 
GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC 
AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT 
TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC 
TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC 
AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG 
CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG 
GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT 
GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC 
TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG 
TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC 
TGGGCCGAGT ATGACCCTGC AGCGTGGGGC CGCATGCCTT ACCCGGACAT GTATCAGATG 
CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC 
AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT 
ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC 
AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC 
CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG 
ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG 
GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG 
CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA 
GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG 
GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT 
ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC 
TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC 
CGCCTGCCTG CCGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC 
ATCTCAGACA CCAGCCCCAT GAAGCGCTCG GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC 
CTGGACGACT ACTCCCTGGA GAGGGTGCCG CCCGAGGAGA ACCAGCGGCA CCACCAGAGG 
CGCAGAGACC GTGGCCACCG CACCTCCGAG CGCTCCTTGG GCCGCTACAC GGACGTGGAC 
ACAGGCTTGG GGACAGACTT GAGCATGACC ACCCAGTCTG GGGACCTGCC ATCAAAGGAA 
AGAGACCAGG AGCGGGGCCG GCCCAAGGAC CGGAAGCATC GGCAACACCA CCACCACCAC 
CACCACCACC ATCCCCCGCC CCCTGACAAG GAGCGCTACA CCCAGGAGCG GCCTGAGCAC 
GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC 
ATGGCCCACC GTCAGGGCAG TAGTTCCGTA AGTGGAAGCC CAGCCCCCTC AACATCTGGT 
ACCAGCACTC CGCGGCGGGG ACGCCGCCAG CTCCCCCAGA CCCCCTCCAC TCCCCGGCCG 
CACGTGTCCT ATTCCCCCGT GATCCGTAAG GCCGGCGGCT CGGGTCCCCA GCAGCAGCAG 
CAGCCCGCGG CGCGGCCGGG CCGAACGGCC CCCAGCGGCC CGCGGAGGTA CCCGGGCCCC 
GTGGCCGAGC CCCTGGCCGG GGAGCGGCCT CCCACGGGAG GCCACGGAAG CAGCCGCTCG 
CCCGCGATGG AGCGGCGGAT CCCGGGCCCG GCCCGGAGCG AGTCCCCCAG GGCGCCGTGT 
CGCCACGGCG GGGCGCGATG GCCGGCGTCC GGCGCGCACG CGGCCGAGGG CCCCCCGGGC 
CCCCGGCACC ACCACGGTTA CTACCGGGGC TCCGACTACG ACGAGCCAGA CGGCCCGGGC 
GGCGGCGGCG GGGAGGAGGA GGAGCCCCGG GCCGCCGCCT ACGACGCGCC GCCCCCCGCG 
CGCCACGGGT GCTCGCCCAG GACTCCCCGG GCCGCGGGCC CGGCCTGCGC CTCGCCTTCC 
AGGCACGGCC GGCGACTCCC GAACGGCTAC TACCCGGCCC ACGGACTGGC CAGGCCCCGC 
GGGCCGGGCT CCCGGAAAGG CCTGCACGAA GCCTACAGCG AGACCGACGA CGATGACTGG 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1A ( XM_024993850.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849618.1 Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X3, mRNA.

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CloneID	OBa235772
Clone ID Related Accession (Same CDS sequence)	XM_024993850.1
Accession Version	XM_024993850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGCTCTG 
CGGAGAGCCA CTATAAAGAA GAGCAAGACA GACCTGCTCA ACCCCGAGGA GGCTGAGGAT 
CAGCTGGCCG ACATCGCCTC TGTGGGGTCT CCCTTCGCCC GAGCCAGCAT TAAAAGTGCC 
AAGCTGGAGA ACTCAACTTT TTTTCACAAA AAGGAGAGGA GGATGCGTTT CTACATCCGC 
CGCATGGTCA AAACTCAGGC CTTCTACTGG ACTGTGCTCA GTCTGGTAGC CCTCAACACG 
CTGTGTGTCG CTATCGTTCA CTACAACCAG CCTGAGTGGC TCTCCGACTT CCTCTACTAT 
GCAGAATTCA TTTTCTTAGG ACTCTTTATG TCCGAAATGT TTATAAAAAT GTACGGGCTT 
GGGACGCGGC CTTACTTCCA CTCTTCCTTC AACTGTTTTG ATTGTGGGGT CATCATCGGG 
AGTATCTTTG AGGTCATCTG GGCTGTCATA AAACCCGGCA CATCCTTTGG AATCAGTGTG 
TTACGAGCCC TCAGGTTATT GCGTATTTTC AAAGTCACAA AGTACTGGGC ATCTCTCAGG 
AACCTGGTCG TCTCTCTTCT CAACTCCATG AAATCCATCA TCAGCCTGTT GTTCCTCCTT 
TTCCTATTCA TCGTCGTCTT TGCCCTTCTG GGAATGCAAC TCTTTGGCGG CCAGTTTAAT 
TTCGATGAAG GGACTCCTCC TACCAACTTC GACACTTTTC CAGCAGCAAT AATGACAGTT 
TTTCAGATCC TGACGGGCGA AGACTGGAAC GAGGTCATGT ATGACGGCAT CAAGTCTCAG 
GGGGGCGTGC AAGGCGGCAT GGTGTTCTCC ATCTACTTCA TCGTGCTGAC GCTCTTCGGG 
AACTACACCC TCCTGAACGT GTTCTTGGCC ATCGCGGTGG ATAATCTGGC TAACGCCCAG 
GAGCTCACCA AGGATGAACA AGAAGAAGAA GAGGCTGCCA ACCAGAAACT CGCCCTCCAG 
AAAGCCAAAG AGGTGGCAGA AGTGAGTCCT CTGTCTGCAG CCAACATGTC CATAGCTGTG 
AAGGAGCAAC AGAAGAACCA GAAGCCCGCC AAGTCGGTGT GGGAGCAGCG GACAAGCGAG 
ATGAGGAAGC AGAATCTTCT GGCCAGTCGG GAGGCACTCT ACAACGAGAT GGACCCAGAC 
GAGCGCTGGA AGGCCTCCTA CGCTCGCCAC CTGCGGCCCG ACATGAAGAC CCACCTGGAC 
CGGCCGCTGG TGGTCGACCC CCAGGAGAAC CGTAACAACA ACACCAACAA GAGCCGGGCG 
GCCGAGCCCA CGGTGGACCA GCGCCTCGGG CAGCAGCGAG CCGAGGACTT CCTCAGGAAA 
CAGGCCCGCT ACCACGACCG GGCCCGGGAC CCCAGCAGCT CCGTGGGCCT GGACACCCGG 
AGGCCCTGGG CGGGCAGCCA GGAAGCCGAG CTGAGCCGGG AGGGGCCTTA CGGTCGCGAG 
TCGGACCACC ACGCCCGGGA GGGCGGCCTG GAGCAGCCGG GGTTCTGGGA GGGCGAGGCG 
GAGCGGGGCA AGGCGGGCGA CCCGCACCGG AGGCACGCGC ACCGGCAGGC GGGCAGCCGC 
GAGAGCCGCA GCGGGTCCCC GCGCACCGGG GCCGACGGAG AGCCCCGCCG GCACCGGGCG 
CACCGCAGGC CGGGCGACGA GGCCCCGGAC GACAAGGCCG ATCGGAGGTC CCGGCACCGC 
GAGGGCAGCC GGCCGGCCCG GGGCGGCGAG GGCGAGAGCG AGGGCCCAGA CGGCGGCGGC 
GAGCGGAGGC GGCGGCACCG GCATGGCCCT CCCCCAGCGT ACGACGCGGA CATGCGCAGG 
GAGGACAAGG AGCGCAGGCA CCGCAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCCGTG 
TCAGGCCCCA ACCTGTCGAC CACACGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCC 
CCGCTGGCCG AGGATATCGA CAACATGAAG AACAACAAAC TGGCCACTGC GGAGTCAGCC 
AGTCCCCACG ACAGCCTCAG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGGAAC 
AGCATGGACC CTGGCCCCAC ACCGGCCCCC ACCACCACGG CCACCAACCC TCAGAATGCC 
GTCAGTCGCC GGACAGCCAA CAACCCGGGG AACCCGTCCA ACCCCGGCCC CCCCAAGACC 
CCTGAGAACA GCCTTATCGT CACCAACCCC AGCAGCACCC AGGCCAACTC AGCTAAGACT 
GCCAGGAAAC CTGACCACAC CACGGTGGAC ATCCCCCCAG CCTGCCCACC CCCACTCAAC 
CACACCATCA TCCAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAGGAGGAA 
GAGAAGAAAG AGGAGGAGGA AGACGACCCC GGGGAAGACG GCCCCAAGCC CATGCCCCCC 
TACAGCTCCA TGTTTATCCT CTCTACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC 
CTGAACCTGC GCTACTTTGA GATGTGCATT CTCATGGTCA TCGCCATGAG CAGCATCGCC 
CTGGCGGCCG AGGATCCTGT TCAGCCCAAC GCACCACGAA ACAATGTGCT GCGATATTTT 
GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT CGACCTGGGG 
CTCGTCCTGC ACCAGGGTGC CTACTTCCGT GACCTCTGGA ACATTCTCGA CTTCATAGTG 
GTCAGTGGGG CCCTGGTGGC CTTTGCCTTC ACGAGCAGTA CACGTGGCAA TAGCAAAGGA 
AAGGACATCA ACACGATTAA ATCCCTCCGA GTCCTCCGGG TGCTACGACC TCTTAAAACC 
ATCAAGCGGT TGCCAAAGCT CAAGGCCGTG TTTGACTGCG TGGTGAACTC TCTCAAGAAT 
GTCTTCAACA TCCTCATCGT CTACATGCTG TTCATGTTCA TCTTCGCCGT GGTGGCCGTG 
CAGCTCTTCA AGGGCAAGTT TTTCCACTGC ACAGATGAGT CCAAGGAGTT TGAGAAAGAT 
TGCCGCGGCA AGTACCTCCT CTATGAGAAG AATGAGGTAA AGGCGAGGGA TCGAGAGTGG 
AAGAAGTATG AGTTCCATTA TGACAATGTG CTGTGGGCGC TGCTGACCCT CTTCACCGTG 
TCCACGGGAG AAGGCTGGCC ACAGGTCCTC AAGCACTCCG TGGACGCCAC CTTCGAGAAC 
CAGGGCCCCA GCCCCGGGTA CCGCATGGAG ATGTCCATCT TCTACGTCGT CTACTTTGTG 
GTGTTCCCCT TCTTCTTTGT CAACATCTTT GTGGCCTTGA TCATCATCAC CTTCCAGGAG 
CAGGGGGACA AGATGATGGA GGAGTACAGC CTGGAGAAAA ATGAGAGGGC GTGCATCGAC 
TTCGCCATCA GCGCCAAACC CCTGACCCGG CACATGCCGC AGAACAAGCA GAGTTTCCAG 
TACCGCATGT GGCAGTTCGT GGTGTCCCCG CCTTTCGAGT ACACCATCAT GGCCATGATC 
GCCCTCAACA CCATCGTGCT CATGATGAAG TTCTACGGGG CCTCTGTTGC TTATGAGAAT 
GCTCTGAGAG TATTCAACAT CGTCTTCACC TCCCTCTTCT CACTGGAATG TGTGCTGAAA 
GTCATGGCTT TTGGGATTCT GAATTATTTT CGCGATGCCT GGAACATCTT CGACTTTGTG 
ACTGTTCTGG GCAGCATCAC CGACATCCTC GTGACCGAGT TTGGGAATAA CTTCATCAAC 
TTGAGCTTCC TCCGCCTCTT CCGAGCTGCC CGGCTCATCA AACTCCTCCG TCAGGGTTAC 
ACCATCCGAA TTCTTCTCTG GACCTTTGTG CAGTCCTTCA AGGCCCTGCC CTATGTCTGT 
CTACTGATTG CCATGCTTTT TTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGCAAC 
ATTGGCATCG ATGTGGAGGA CGAGGACAGT GATGAAGATG AATTCCAAAT CACTGAGCAC 
AATAACTTCC GCACCTTCTT CCAGGCCCTC ATGCTTCTCT TCCGGAGTGC CACTGGGGAG 
GCTTGGCACA ACATCATGCT TTCCTGCCTC AGCGGGAAAC CCTGTGATAA GAACTCTGGC 
ATCCTGACTC ACGAATGCGG CAATGAATTT GCTTATTTTT ACTTCGTTTC CTTCATCTTC 
CTCTGCTCGT TTCTGATGCT GAATCTTTTT GTTGCCGTCA TCATGGACAA CTTTGAGTAC 
CTCACCCGAG ATTCCTCCAT CCTGGGTCCC CACCACCTGG ATGAGTACGT GCGTGTCTGG 
GCCGAGTATG ACCCTGCAGC GTGCGGTCGG ATTCATTATA AGGATATGTA CAGTTTATTG 
CGCGTAATAT CTCCCCCTCT CGGCTTAGGC AAGAAATGTC CTCATAGGGT TGCTTGCAAG 
AGGCTCCTGC GGATGGATCT GCCCGTCGCT GATGACAACA CCGTCCACTT CAACTCTACC 
CTCATGGCTC TGATCCGCAC GGCCCTGGAC ATCAAAATTG CCAAAGGTGG AGCTGACAAA 
CAACAAATGG ATGCTGAGTT GCGGAAGGAG ATGATGGCCA TCTGGCCCAA TCTGTCCCAG 
AAGACGCTGG ACCTGCTGGT CACCCCCCAC AAGTCCACGG ACCTGACAGT GGGGAAGATC 
TATGCGGCCA TGATGATCAT GGAGTATTAC CGGCAGAGCA AGGCCAAGAA GCTTCAGGCC 
ATGCGCGAGG AACAGAACCG GACACCTCTC ATGTTCCAGC GCATGGAGCC CCCATCGCCA 
ACACAGGAGG GGGGGCCTGG CCAGAACGCC CTCCCCTCCA CCCAGCTGGA CCCAGGAGGA 
GGCTTGATGG CTCACGAAAG TGGCATGAAA GAGAGCCCAT CCTGGGTGAC CCAGCGGGCC 
CAGGAGATGT TCCAGAAGAC GGGCACATGG AGCCCAGAGC GGGGCCCCCC CACTGATATG 
CCTAATAGCC AACCCAACTC TCAGTCTGTG GAGATGCGAG AGATGGGCAG AGACGGCTAC 
TCTGACAGTG AGCACTACTT CCCCGTTGAA GGCCAGGCCC GGGCCGCCTC CATGCCCCGC 
CTGCCTGCCG AGAACCAGAG GAGAAGGGGC CGGCCACGTG GGAATAACCT CAGTACCATC 
TCAGACACCA GCCCCATGAA GCGCTCGGCC TCCGTGCTGG GCCCCAAGGC CCGGCGCCTG 
GACGACTACT CCCTGGAGAG GGTGCCGCCC GAGGAGAACC AGCGGCACCA CCAGAGGCGC 
AGAGACCGTG GCCACCGCAC CTCCGAGCGC TCCTTGGGCC GCTACACGGA CGTGGACACA 
GGCTTGGGGA CAGACTTGAG CATGACCACC CAGTCTGGGG ACCTGCCATC AAAGGAAAGA 
GACCAGGAGC GGGGCCGGCC CAAGGACCGG AAGCATCGGC AACACCACCA CCACCACCAC 
CACCACCATC CCCCGCCCCC TGACAAGGAG CGCTACACCC AGGAGCGGCC TGAGCACGGC 
CGGCCCCGGG CCCGTGACCA GCGCTGGTCC CGCTCACCCA GTGAGGGCCG GGAGCACATG 
GCCCACCGTC AGGGCAGTAG TTCCGTAAGT GGAAGCCCAG CCCCCTCAAC ATCTGGTACC 
AGCACTCCGC GGCGGGGACG CCGCCAGCTC CCCCAGACCC CCTCCACTCC CCGGCCGCAC 
GTGTCCTATT CCCCCGTGAT CCGTAAGGCC GGCGGCTCGG GTCCCCAGCA GCAGCAGCAG 
CCCGCGGCGC GGCCGGGCCG AACGGCCCCC AGCGGCCCGC GGAGGTACCC GGGCCCCGTG 
GCCGAGCCCC TGGCCGGGGA GCGGCCTCCC ACGGGAGGCC ACGGAAGCAG CCGCTCGCCC 
GCGATGGAGC GGCGGATCCC GGGCCCGGCC CGGAGCGAGT CCCCCAGGGC GCCGTGTCGC 
CACGGCGGGG CGCGATGGCC GGCGTCCGGC GCGCACGCGG CCGAGGGCCC CCCGGGCCCC 
CGGCACCACC ACGGTTACTA CCGGGGCTCC GACTACGACG AGCCAGACGG CCCGGGCGGC 
GGCGGCGGGG AGGAGGAGGA GCCCCGGGCC GCCGCCTACG ACGCGCCGCC CCCCGCGCGC 
CACGGGTGCT CGCCCAGGAC TCCCCGGGCC GCGGGCCCGG CCTGCGCCTC GCCTTCCAGG 
CACGGCCGGC GACTCCCGAA CGGCTACTAC CCGGCCCACG GACTGGCCAG GCCCCGCGGG 
CCGGGCTCCC GGAAAGGCCT GCACGAAGCC TACAGCGAGA CCGACGACGA TGACTGGTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849618.1
CDS	230..7732
Translation	MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRHPFDALRRATIKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSSSVGLDTRRPWAGSQEAELSREGPYGRESDHHAREGGLEQPGFWEGEAERGKAGDPHRRHAHRQAGSRESRSGSPRTGADGEPRRHRAHRRPGDEAPDDKADRRSRHREGSRPARGGEGESEGPDGGGERRRRHRHGPPPAYDADMRREDKERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESASPHDSLSHAGLPQSPAKMGNSMDPGPTPAPTTTATNPQNAVSRRTANNPGNPSNPGPPKTPENSLIVTNPSSTQANSAKTARKPDHTTVDIPPACPPPLNHTIIQVNKNANPDPLPKKEEEKKEEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTHECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPGQNALPSTQLDPGGGLMAHESGMKESPSWVTQRAQEMFQKTGTWSPERGPPTDMPNSQPNSQSVEMREMGRDGYSDSEHYFPVEGQARAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRPKDRKHRQHHHHHHHHHPPPPDKERYTQERPEHGRPRARDQRWSRSPSEGREHMAHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPSTPRPHVSYSPVIRKAGGSGPQQQQQPAARPGRTAPSGPRRYPGPVAEPLAGERPPTGGHGSSRSPAMERRIPGPARSESPRAPCRHGGARWPASGAHAAEGPPGPRHHHGYYRGSDYDEPDGPGGGGGEEEEPRAAAYDAPPPARHGCSPRTPRAAGPACASPSRHGRRLPNGYYPAHGLARPRGPGSRKGLHEAYSETDDDDWC

Target ORF information:

RefSeq Version	XM_024993850.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993850.1

ORF Insert Sequence:

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGCTCTG 
CGGAGAGCCA CTATAAAGAA GAGCAAGACA GACCTGCTCA ACCCCGAGGA GGCTGAGGAT 
CAGCTGGCCG ACATCGCCTC TGTGGGGTCT CCCTTCGCCC GAGCCAGCAT TAAAAGTGCC 
AAGCTGGAGA ACTCAACTTT TTTTCACAAA AAGGAGAGGA GGATGCGTTT CTACATCCGC 
CGCATGGTCA AAACTCAGGC CTTCTACTGG ACTGTGCTCA GTCTGGTAGC CCTCAACACG 
CTGTGTGTCG CTATCGTTCA CTACAACCAG CCTGAGTGGC TCTCCGACTT CCTCTACTAT 
GCAGAATTCA TTTTCTTAGG ACTCTTTATG TCCGAAATGT TTATAAAAAT GTACGGGCTT 
GGGACGCGGC CTTACTTCCA CTCTTCCTTC AACTGTTTTG ATTGTGGGGT CATCATCGGG 
AGTATCTTTG AGGTCATCTG GGCTGTCATA AAACCCGGCA CATCCTTTGG AATCAGTGTG 
TTACGAGCCC TCAGGTTATT GCGTATTTTC AAAGTCACAA AGTACTGGGC ATCTCTCAGG 
AACCTGGTCG TCTCTCTTCT CAACTCCATG AAATCCATCA TCAGCCTGTT GTTCCTCCTT 
TTCCTATTCA TCGTCGTCTT TGCCCTTCTG GGAATGCAAC TCTTTGGCGG CCAGTTTAAT 
TTCGATGAAG GGACTCCTCC TACCAACTTC GACACTTTTC CAGCAGCAAT AATGACAGTT 
TTTCAGATCC TGACGGGCGA AGACTGGAAC GAGGTCATGT ATGACGGCAT CAAGTCTCAG 
GGGGGCGTGC AAGGCGGCAT GGTGTTCTCC ATCTACTTCA TCGTGCTGAC GCTCTTCGGG 
AACTACACCC TCCTGAACGT GTTCTTGGCC ATCGCGGTGG ATAATCTGGC TAACGCCCAG 
GAGCTCACCA AGGATGAACA AGAAGAAGAA GAGGCTGCCA ACCAGAAACT CGCCCTCCAG 
AAAGCCAAAG AGGTGGCAGA AGTGAGTCCT CTGTCTGCAG CCAACATGTC CATAGCTGTG 
AAGGAGCAAC AGAAGAACCA GAAGCCCGCC AAGTCGGTGT GGGAGCAGCG GACAAGCGAG 
ATGAGGAAGC AGAATCTTCT GGCCAGTCGG GAGGCACTCT ACAACGAGAT GGACCCAGAC 
GAGCGCTGGA AGGCCTCCTA CGCTCGCCAC CTGCGGCCCG ACATGAAGAC CCACCTGGAC 
CGGCCGCTGG TGGTCGACCC CCAGGAGAAC CGTAACAACA ACACCAACAA GAGCCGGGCG 
GCCGAGCCCA CGGTGGACCA GCGCCTCGGG CAGCAGCGAG CCGAGGACTT CCTCAGGAAA 
CAGGCCCGCT ACCACGACCG GGCCCGGGAC CCCAGCAGCT CCGTGGGCCT GGACACCCGG 
AGGCCCTGGG CGGGCAGCCA GGAAGCCGAG CTGAGCCGGG AGGGGCCTTA CGGTCGCGAG 
TCGGACCACC ACGCCCGGGA GGGCGGCCTG GAGCAGCCGG GGTTCTGGGA GGGCGAGGCG 
GAGCGGGGCA AGGCGGGCGA CCCGCACCGG AGGCACGCGC ACCGGCAGGC GGGCAGCCGC 
GAGAGCCGCA GCGGGTCCCC GCGCACCGGG GCCGACGGAG AGCCCCGCCG GCACCGGGCG 
CACCGCAGGC CGGGCGACGA GGCCCCGGAC GACAAGGCCG ATCGGAGGTC CCGGCACCGC 
GAGGGCAGCC GGCCGGCCCG GGGCGGCGAG GGCGAGAGCG AGGGCCCAGA CGGCGGCGGC 
GAGCGGAGGC GGCGGCACCG GCATGGCCCT CCCCCAGCGT ACGACGCGGA CATGCGCAGG 
GAGGACAAGG AGCGCAGGCA CCGCAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCCGTG 
TCAGGCCCCA ACCTGTCGAC CACACGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCC 
CCGCTGGCCG AGGATATCGA CAACATGAAG AACAACAAAC TGGCCACTGC GGAGTCAGCC 
AGTCCCCACG ACAGCCTCAG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGGAAC 
AGCATGGACC CTGGCCCCAC ACCGGCCCCC ACCACCACGG CCACCAACCC TCAGAATGCC 
GTCAGTCGCC GGACAGCCAA CAACCCGGGG AACCCGTCCA ACCCCGGCCC CCCCAAGACC 
CCTGAGAACA GCCTTATCGT CACCAACCCC AGCAGCACCC AGGCCAACTC AGCTAAGACT 
GCCAGGAAAC CTGACCACAC CACGGTGGAC ATCCCCCCAG CCTGCCCACC CCCACTCAAC 
CACACCATCA TCCAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAGGAGGAA 
GAGAAGAAAG AGGAGGAGGA AGACGACCCC GGGGAAGACG GCCCCAAGCC CATGCCCCCC 
TACAGCTCCA TGTTTATCCT CTCTACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC 
CTGAACCTGC GCTACTTTGA GATGTGCATT CTCATGGTCA TCGCCATGAG CAGCATCGCC 
CTGGCGGCCG AGGATCCTGT TCAGCCCAAC GCACCACGAA ACAATGTGCT GCGATATTTT 
GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT CGACCTGGGG 
CTCGTCCTGC ACCAGGGTGC CTACTTCCGT GACCTCTGGA ACATTCTCGA CTTCATAGTG 
GTCAGTGGGG CCCTGGTGGC CTTTGCCTTC ACGAGCAGTA CACGTGGCAA TAGCAAAGGA 
AAGGACATCA ACACGATTAA ATCCCTCCGA GTCCTCCGGG TGCTACGACC TCTTAAAACC 
ATCAAGCGGT TGCCAAAGCT CAAGGCCGTG TTTGACTGCG TGGTGAACTC TCTCAAGAAT 
GTCTTCAACA TCCTCATCGT CTACATGCTG TTCATGTTCA TCTTCGCCGT GGTGGCCGTG 
CAGCTCTTCA AGGGCAAGTT TTTCCACTGC ACAGATGAGT CCAAGGAGTT TGAGAAAGAT 
TGCCGCGGCA AGTACCTCCT CTATGAGAAG AATGAGGTAA AGGCGAGGGA TCGAGAGTGG 
AAGAAGTATG AGTTCCATTA TGACAATGTG CTGTGGGCGC TGCTGACCCT CTTCACCGTG 
TCCACGGGAG AAGGCTGGCC ACAGGTCCTC AAGCACTCCG TGGACGCCAC CTTCGAGAAC 
CAGGGCCCCA GCCCCGGGTA CCGCATGGAG ATGTCCATCT TCTACGTCGT CTACTTTGTG 
GTGTTCCCCT TCTTCTTTGT CAACATCTTT GTGGCCTTGA TCATCATCAC CTTCCAGGAG 
CAGGGGGACA AGATGATGGA GGAGTACAGC CTGGAGAAAA ATGAGAGGGC GTGCATCGAC 
TTCGCCATCA GCGCCAAACC CCTGACCCGG CACATGCCGC AGAACAAGCA GAGTTTCCAG 
TACCGCATGT GGCAGTTCGT GGTGTCCCCG CCTTTCGAGT ACACCATCAT GGCCATGATC 
GCCCTCAACA CCATCGTGCT CATGATGAAG TTCTACGGGG CCTCTGTTGC TTATGAGAAT 
GCTCTGAGAG TATTCAACAT CGTCTTCACC TCCCTCTTCT CACTGGAATG TGTGCTGAAA 
GTCATGGCTT TTGGGATTCT GAATTATTTT CGCGATGCCT GGAACATCTT CGACTTTGTG 
ACTGTTCTGG GCAGCATCAC CGACATCCTC GTGACCGAGT TTGGGAATAA CTTCATCAAC 
TTGAGCTTCC TCCGCCTCTT CCGAGCTGCC CGGCTCATCA AACTCCTCCG TCAGGGTTAC 
ACCATCCGAA TTCTTCTCTG GACCTTTGTG CAGTCCTTCA AGGCCCTGCC CTATGTCTGT 
CTACTGATTG CCATGCTTTT TTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGCAAC 
ATTGGCATCG ATGTGGAGGA CGAGGACAGT GATGAAGATG AATTCCAAAT CACTGAGCAC 
AATAACTTCC GCACCTTCTT CCAGGCCCTC ATGCTTCTCT TCCGGAGTGC CACTGGGGAG 
GCTTGGCACA ACATCATGCT TTCCTGCCTC AGCGGGAAAC CCTGTGATAA GAACTCTGGC 
ATCCTGACTC ACGAATGCGG CAATGAATTT GCTTATTTTT ACTTCGTTTC CTTCATCTTC 
CTCTGCTCGT TTCTGATGCT GAATCTTTTT GTTGCCGTCA TCATGGACAA CTTTGAGTAC 
CTCACCCGAG ATTCCTCCAT CCTGGGTCCC CACCACCTGG ATGAGTACGT GCGTGTCTGG 
GCCGAGTATG ACCCTGCAGC GTGCGGTCGG ATTCATTATA AGGATATGTA CAGTTTATTG 
CGCGTAATAT CTCCCCCTCT CGGCTTAGGC AAGAAATGTC CTCATAGGGT TGCTTGCAAG 
AGGCTCCTGC GGATGGATCT GCCCGTCGCT GATGACAACA CCGTCCACTT CAACTCTACC 
CTCATGGCTC TGATCCGCAC GGCCCTGGAC ATCAAAATTG CCAAAGGTGG AGCTGACAAA 
CAACAAATGG ATGCTGAGTT GCGGAAGGAG ATGATGGCCA TCTGGCCCAA TCTGTCCCAG 
AAGACGCTGG ACCTGCTGGT CACCCCCCAC AAGTCCACGG ACCTGACAGT GGGGAAGATC 
TATGCGGCCA TGATGATCAT GGAGTATTAC CGGCAGAGCA AGGCCAAGAA GCTTCAGGCC 
ATGCGCGAGG AACAGAACCG GACACCTCTC ATGTTCCAGC GCATGGAGCC CCCATCGCCA 
ACACAGGAGG GGGGGCCTGG CCAGAACGCC CTCCCCTCCA CCCAGCTGGA CCCAGGAGGA 
GGCTTGATGG CTCACGAAAG TGGCATGAAA GAGAGCCCAT CCTGGGTGAC CCAGCGGGCC 
CAGGAGATGT TCCAGAAGAC GGGCACATGG AGCCCAGAGC GGGGCCCCCC CACTGATATG 
CCTAATAGCC AACCCAACTC TCAGTCTGTG GAGATGCGAG AGATGGGCAG AGACGGCTAC 
TCTGACAGTG AGCACTACTT CCCCGTTGAA GGCCAGGCCC GGGCCGCCTC CATGCCCCGC 
CTGCCTGCCG AGAACCAGAG GAGAAGGGGC CGGCCACGTG GGAATAACCT CAGTACCATC 
TCAGACACCA GCCCCATGAA GCGCTCGGCC TCCGTGCTGG GCCCCAAGGC CCGGCGCCTG 
GACGACTACT CCCTGGAGAG GGTGCCGCCC GAGGAGAACC AGCGGCACCA CCAGAGGCGC 
AGAGACCGTG GCCACCGCAC CTCCGAGCGC TCCTTGGGCC GCTACACGGA CGTGGACACA 
GGCTTGGGGA CAGACTTGAG CATGACCACC CAGTCTGGGG ACCTGCCATC AAAGGAAAGA 
GACCAGGAGC GGGGCCGGCC CAAGGACCGG AAGCATCGGC AACACCACCA CCACCACCAC 
CACCACCATC CCCCGCCCCC TGACAAGGAG CGCTACACCC AGGAGCGGCC TGAGCACGGC 
CGGCCCCGGG CCCGTGACCA GCGCTGGTCC CGCTCACCCA GTGAGGGCCG GGAGCACATG 
GCCCACCGTC AGGGCAGTAG TTCCGTAAGT GGAAGCCCAG CCCCCTCAAC ATCTGGTACC 
AGCACTCCGC GGCGGGGACG CCGCCAGCTC CCCCAGACCC CCTCCACTCC CCGGCCGCAC 
GTGTCCTATT CCCCCGTGAT CCGTAAGGCC GGCGGCTCGG GTCCCCAGCA GCAGCAGCAG 
CCCGCGGCGC GGCCGGGCCG AACGGCCCCC AGCGGCCCGC GGAGGTACCC GGGCCCCGTG 
GCCGAGCCCC TGGCCGGGGA GCGGCCTCCC ACGGGAGGCC ACGGAAGCAG CCGCTCGCCC 
GCGATGGAGC GGCGGATCCC GGGCCCGGCC CGGAGCGAGT CCCCCAGGGC GCCGTGTCGC 
CACGGCGGGG CGCGATGGCC GGCGTCCGGC GCGCACGCGG CCGAGGGCCC CCCGGGCCCC 
CGGCACCACC ACGGTTACTA CCGGGGCTCC GACTACGACG AGCCAGACGG CCCGGGCGGC 
GGCGGCGGGG AGGAGGAGGA GCCCCGGGCC GCCGCCTACG ACGCGCCGCC CCCCGCGCGC 
CACGGGTGCT CGCCCAGGAC TCCCCGGGCC GCGGGCCCGG CCTGCGCCTC GCCTTCCAGG 
CACGGCCGGC GACTCCCGAA CGGCTACTAC CCGGCCCACG GACTGGCCAG GCCCCGCGGG 
CCGGGCTCCC GGAAAGGCCT GCACGAAGCC TACAGCGAGA CCGACGACGA TGACTGGTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1A ( XM_024993851.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849619.1 Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X4, mRNA.

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CloneID	OBa235773
Clone ID Related Accession (Same CDS sequence)	XM_024993851.1
Accession Version	XM_024993851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACTGGCA ATAGCAAAGG AAAGGACATC 
AACACGATTA AATCCCTCCG AGTCCTCCGG GTGCTACGAC CTCTTAAAAC CATCAAGCGG 
TTGCCAAAGC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTCAAGAA TGTCTTCAAC 
ATCCTCATCG TCTACATGCT GTTCATGTTC ATCTTCGCCG TGGTGGCCGT GCAGCTCTTC 
AAGGGCAAGT TTTTCCACTG CACAGATGAG TCCAAGGAGT TTGAGAAAGA TTGCCGCGGC 
AAGTACCTCC TCTATGAGAA GAATGAGGTA AAGGCGAGGG ATCGAGAGTG GAAGAAGTAT 
GAGTTCCATT ATGACAATGT GCTGTGGGCG CTGCTGACCC TCTTCACCGT GTCCACGGGA 
GAAGGCTGGC CACAGGTCCT CAAGCACTCC GTGGACGCCA CCTTCGAGAA CCAGGGCCCC 
AGCCCCGGGT ACCGCATGGA GATGTCCATC TTCTACGTCG TCTACTTTGT GGTGTTCCCC 
TTCTTCTTTG TCAACATCTT TGTGGCCTTG ATCATCATCA CCTTCCAGGA GCAGGGGGAC 
AAGATGATGG AGGAGTACAG CCTGGAGAAA AATGAGAGGG CGTGCATCGA CTTCGCCATC 
AGCGCCAAAC CCCTGACCCG GCACATGCCG CAGAACAAGC AGAGTTTCCA GTACCGCATG 
TGGCAGTTCG TGGTGTCCCC GCCTTTCGAG TACACCATCA TGGCCATGAT CGCCCTCAAC 
ACCATCGTGC TCATGATGAA GTTCTACGGG GCCTCTGTTG CTTATGAGAA TGCTCTGAGA 
GTATTCAACA TCGTCTTCAC CTCCCTCTTC TCACTGGAAT GTGTGCTGAA AGTCATGGCT 
TTTGGGATTC TGAATTATTT TCGCGATGCC TGGAACATCT TCGACTTTGT GACTGTTCTG 
GGCAGCATCA CCGACATCCT CGTGACCGAG TTTGGGAATA ACTTCATCAA CTTGAGCTTC 
CTCCGCCTCT TCCGAGCTGC CCGGCTCATC AAACTCCTCC GTCAGGGTTA CACCATCCGA 
ATTCTTCTCT GGACCTTTGT GCAGTCCTTC AAGGCCCTGC CCTATGTCTG TCTACTGATT 
GCCATGCTTT TTTTCATCTA CGCCATCATT GGGATGCAGG TGTTTGGCAA CATTGGCATC 
GATGTGGAGG ACGAGGACAG TGATGAAGAT GAATTCCAAA TCACTGAGCA CAATAACTTC 
CGCACCTTCT TCCAGGCCCT CATGCTTCTC TTCCGGAGTG CCACTGGGGA GGCTTGGCAC 
AACATCATGC TTTCCTGCCT CAGCGGGAAA CCCTGTGATA AGAACTCTGG CATCCTGACT 
CACGAATGCG GCAATGAATT TGCTTATTTT TACTTCGTTT CCTTCATCTT CCTCTGCTCG 
TTTCTGATGC TGAATCTTTT TGTTGCCGTC ATCATGGACA ACTTTGAGTA CCTCACCCGA 
GATTCCTCCA TCCTGGGTCC CCACCACCTG GATGAGTACG TGCGTGTCTG GGCCGAGTAT 
GACCCTGCAG CGTGCGGTCG GATTCATTAT AAGGATATGT ACAGTTTATT GCGCGTAATA 
TCTCCCCCTC TCGGCTTAGG CAAGAAATGT CCTCATAGGG TTGCTTGCAA GAGGCTCCTG 
CGGATGGATC TGCCCGTCGC TGATGACAAC ACCGTCCACT TCAACTCTAC CCTCATGGCT 
CTGATCCGCA CGGCCCTGGA CATCAAAATT GCCAAAGGTG GAGCTGACAA ACAACAAATG 
GATGCTGAGT TGCGGAAGGA GATGATGGCC ATCTGGCCCA ATCTGTCCCA GAAGACGCTG 
GACCTGCTGG TCACCCCCCA CAAGTCCACG GACCTGACAG TGGGGAAGAT CTATGCGGCC 
ATGATGATCA TGGAGTATTA CCGGCAGAGC AAGGCCAAGA AGCTTCAGGC CATGCGCGAG 
GAACAGAACC GGACACCTCT CATGTTCCAG CGCATGGAGC CCCCATCGCC AACACAGGAG 
GGGGGGCCTG GCCAGAACGC CCTCCCCTCC ACCCAGCTGG ACCCAGGAGG AGGCTTGATG 
GCTCACGAAA GTGGCATGAA AGAGAGCCCA TCCTGGGTGA CCCAGCGGGC CCAGGAGATG 
TTCCAGAAGA CGGGCACATG GAGCCCAGAG CGGGGCCCCC CCACTGATAT GCCTAATAGC 
CAACCCAACT CTCAGTCTGT GGAGATGCGA GAGATGGGCA GAGACGGCTA CTCTGACAGT 
GAGCACTACT TCCCCGTTGA AGGCCAGGCC CGGGCCGCCT CCATGCCCCG CCTGCCTGCC 
GAGAACCAGA GGAGAAGGGG CCGGCCACGT GGGAATAACC TCAGTACCAT CTCAGACACC 
AGCCCCATGA AGCGCTCGGC CTCCGTGCTG GGCCCCAAGG CCCGGCGCCT GGACGACTAC 
TCCCTGGAGA GGGTGCCGCC CGAGGAGAAC CAGCGGCACC ACCAGAGGCG CAGAGACCGT 
GGCCACCGCA CCTCCGAGCG CTCCTTGGGC CGCTACACGG ACGTGGACAC AGGCTTGGGG 
ACAGACTTGA GCATGACCAC CCAGTCTGGG GACCTGCCAT CAAAGGAAAG AGACCAGGAG 
CGGGGCCGGC CCAAGGACCG GAAGCATCGG CAACACCACC ACCACCACCA CCACCACCAT 
CCCCCGCCCC CTGACAAGGA GCGCTACACC CAGGAGCGGC CTGAGCACGG CCGGCCCCGG 
GCCCGTGACC AGCGCTGGTC CCGCTCACCC AGTGAGGGCC GGGAGCACAT GGCCCACCGT 
CAGGGCAGTA GTTCCGTAAG TGGAAGCCCA GCCCCCTCAA CATCTGGTAC CAGCACTCCG 
CGGCGGGGAC GCCGCCAGCT CCCCCAGACC CCCTCCACTC CCCGGCCGCA CGTGTCCTAT 
TCCCCCGTGA TCCGTAAGGC CGGCGGCTCG GGTCCCCAGC AGCAGCAGCA GCCCGCGGCG 
CGGCCGGGCC GAACGGCCCC CAGCGGCCCG CGGAGGTACC CGGGCCCCGT GGCCGAGCCC 
CTGGCCGGGG AGCGGCCTCC CACGGGAGGC CACGGAAGCA GCCGCTCGCC CGCGATGGAG 
CGGCGGATCC CGGGCCCGGC CCGGAGCGAG TCCCCCAGGG CGCCGTGTCG CCACGGCGGG 
GCGCGATGGC CGGCGTCCGG CGCGCACGCG GCCGAGGGCC CCCCGGGCCC CCGGCACCAC 
CACGGTTACT ACCGGGGCTC CGACTACGAC GAGCCAGACG GCCCGGGCGG CGGCGGCGGG 
GAGGAGGAGG AGCCCCGGGC CGCCGCCTAC GACGCGCCGC CCCCCGCGCG CCACGGGTGC 
TCGCCCAGGA CTCCCCGGGC CGCGGGCCCG GCCTGCGCCT CGCCTTCCAG GCACGGCCGG 
CGACTCCCGA ACGGCTACTA CCCGGCCCAC GGACTGGCCA GGCCCCGCGG GCCGGGCTCC 
CGGAAAGGCC TGCACGAAGC CTACAGCGAG ACCGACGACG ATGACTGGTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849619.1
CDS	230..7723
Translation	MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRHPFDGALRRATIKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSSSVGLDTRRPWAGSQEAELSREGPYGRESDHHAREGGLEQPGFWEGEAERGKAGDPHRRHAHRQAGSRESRSGSPRTGADGEPRRHRAHRRPGDEAPDDKADRRSRHREGSRPARGGEGESEGPDGGGERRRRHRHGPPPAYDADMRREDKERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESASPHDSLSHAGLPQSPAKMGNSMDPGPTPAPTTTATNPQNAVSRRTANNPGNPSNPGPPKTPENSLIVTNPSSTQANSAKTARKPDHTTVDIPPACPPPLNHTIIQVNKNANPDPLPKKEEEKKEEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTHECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPGQNALPSTQLDPGGGLMAHESGMKESPSWVTQRAQEMFQKTGTWSPERGPPTDMPNSQPNSQSVEMREMGRDGYSDSEHYFPVEGQARAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRPKDRKHRQHHHHHHHHHPPPPDKERYTQERPEHGRPRARDQRWSRSPSEGREHMAHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPSTPRPHVSYSPVIRKAGGSGPQQQQQPAARPGRTAPSGPRRYPGPVAEPLAGERPPTGGHGSSRSPAMERRIPGPARSESPRAPCRHGGARWPASGAHAAEGPPGPRHHHGYYRGSDYDEPDGPGGGGGEEEEPRAAAYDAPPPARHGCSPRTPRAAGPACASPSRHGRRLPNGYYPAHGLARPRGPGSRKGLHEAYSETDDDDWC

Target ORF information:

RefSeq Version	XM_024993851.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993851.1

ORF Insert Sequence:

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACTGGCA ATAGCAAAGG AAAGGACATC 
AACACGATTA AATCCCTCCG AGTCCTCCGG GTGCTACGAC CTCTTAAAAC CATCAAGCGG 
TTGCCAAAGC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTCAAGAA TGTCTTCAAC 
ATCCTCATCG TCTACATGCT GTTCATGTTC ATCTTCGCCG TGGTGGCCGT GCAGCTCTTC 
AAGGGCAAGT TTTTCCACTG CACAGATGAG TCCAAGGAGT TTGAGAAAGA TTGCCGCGGC 
AAGTACCTCC TCTATGAGAA GAATGAGGTA AAGGCGAGGG ATCGAGAGTG GAAGAAGTAT 
GAGTTCCATT ATGACAATGT GCTGTGGGCG CTGCTGACCC TCTTCACCGT GTCCACGGGA 
GAAGGCTGGC CACAGGTCCT CAAGCACTCC GTGGACGCCA CCTTCGAGAA CCAGGGCCCC 
AGCCCCGGGT ACCGCATGGA GATGTCCATC TTCTACGTCG TCTACTTTGT GGTGTTCCCC 
TTCTTCTTTG TCAACATCTT TGTGGCCTTG ATCATCATCA CCTTCCAGGA GCAGGGGGAC 
AAGATGATGG AGGAGTACAG CCTGGAGAAA AATGAGAGGG CGTGCATCGA CTTCGCCATC 
AGCGCCAAAC CCCTGACCCG GCACATGCCG CAGAACAAGC AGAGTTTCCA GTACCGCATG 
TGGCAGTTCG TGGTGTCCCC GCCTTTCGAG TACACCATCA TGGCCATGAT CGCCCTCAAC 
ACCATCGTGC TCATGATGAA GTTCTACGGG GCCTCTGTTG CTTATGAGAA TGCTCTGAGA 
GTATTCAACA TCGTCTTCAC CTCCCTCTTC TCACTGGAAT GTGTGCTGAA AGTCATGGCT 
TTTGGGATTC TGAATTATTT TCGCGATGCC TGGAACATCT TCGACTTTGT GACTGTTCTG 
GGCAGCATCA CCGACATCCT CGTGACCGAG TTTGGGAATA ACTTCATCAA CTTGAGCTTC 
CTCCGCCTCT TCCGAGCTGC CCGGCTCATC AAACTCCTCC GTCAGGGTTA CACCATCCGA 
ATTCTTCTCT GGACCTTTGT GCAGTCCTTC AAGGCCCTGC CCTATGTCTG TCTACTGATT 
GCCATGCTTT TTTTCATCTA CGCCATCATT GGGATGCAGG TGTTTGGCAA CATTGGCATC 
GATGTGGAGG ACGAGGACAG TGATGAAGAT GAATTCCAAA TCACTGAGCA CAATAACTTC 
CGCACCTTCT TCCAGGCCCT CATGCTTCTC TTCCGGAGTG CCACTGGGGA GGCTTGGCAC 
AACATCATGC TTTCCTGCCT CAGCGGGAAA CCCTGTGATA AGAACTCTGG CATCCTGACT 
CACGAATGCG GCAATGAATT TGCTTATTTT TACTTCGTTT CCTTCATCTT CCTCTGCTCG 
TTTCTGATGC TGAATCTTTT TGTTGCCGTC ATCATGGACA ACTTTGAGTA CCTCACCCGA 
GATTCCTCCA TCCTGGGTCC CCACCACCTG GATGAGTACG TGCGTGTCTG GGCCGAGTAT 
GACCCTGCAG CGTGCGGTCG GATTCATTAT AAGGATATGT ACAGTTTATT GCGCGTAATA 
TCTCCCCCTC TCGGCTTAGG CAAGAAATGT CCTCATAGGG TTGCTTGCAA GAGGCTCCTG 
CGGATGGATC TGCCCGTCGC TGATGACAAC ACCGTCCACT TCAACTCTAC CCTCATGGCT 
CTGATCCGCA CGGCCCTGGA CATCAAAATT GCCAAAGGTG GAGCTGACAA ACAACAAATG 
GATGCTGAGT TGCGGAAGGA GATGATGGCC ATCTGGCCCA ATCTGTCCCA GAAGACGCTG 
GACCTGCTGG TCACCCCCCA CAAGTCCACG GACCTGACAG TGGGGAAGAT CTATGCGGCC 
ATGATGATCA TGGAGTATTA CCGGCAGAGC AAGGCCAAGA AGCTTCAGGC CATGCGCGAG 
GAACAGAACC GGACACCTCT CATGTTCCAG CGCATGGAGC CCCCATCGCC AACACAGGAG 
GGGGGGCCTG GCCAGAACGC CCTCCCCTCC ACCCAGCTGG ACCCAGGAGG AGGCTTGATG 
GCTCACGAAA GTGGCATGAA AGAGAGCCCA TCCTGGGTGA CCCAGCGGGC CCAGGAGATG 
TTCCAGAAGA CGGGCACATG GAGCCCAGAG CGGGGCCCCC CCACTGATAT GCCTAATAGC 
CAACCCAACT CTCAGTCTGT GGAGATGCGA GAGATGGGCA GAGACGGCTA CTCTGACAGT 
GAGCACTACT TCCCCGTTGA AGGCCAGGCC CGGGCCGCCT CCATGCCCCG CCTGCCTGCC 
GAGAACCAGA GGAGAAGGGG CCGGCCACGT GGGAATAACC TCAGTACCAT CTCAGACACC 
AGCCCCATGA AGCGCTCGGC CTCCGTGCTG GGCCCCAAGG CCCGGCGCCT GGACGACTAC 
TCCCTGGAGA GGGTGCCGCC CGAGGAGAAC CAGCGGCACC ACCAGAGGCG CAGAGACCGT 
GGCCACCGCA CCTCCGAGCG CTCCTTGGGC CGCTACACGG ACGTGGACAC AGGCTTGGGG 
ACAGACTTGA GCATGACCAC CCAGTCTGGG GACCTGCCAT CAAAGGAAAG AGACCAGGAG 
CGGGGCCGGC CCAAGGACCG GAAGCATCGG CAACACCACC ACCACCACCA CCACCACCAT 
CCCCCGCCCC CTGACAAGGA GCGCTACACC CAGGAGCGGC CTGAGCACGG CCGGCCCCGG 
GCCCGTGACC AGCGCTGGTC CCGCTCACCC AGTGAGGGCC GGGAGCACAT GGCCCACCGT 
CAGGGCAGTA GTTCCGTAAG TGGAAGCCCA GCCCCCTCAA CATCTGGTAC CAGCACTCCG 
CGGCGGGGAC GCCGCCAGCT CCCCCAGACC CCCTCCACTC CCCGGCCGCA CGTGTCCTAT 
TCCCCCGTGA TCCGTAAGGC CGGCGGCTCG GGTCCCCAGC AGCAGCAGCA GCCCGCGGCG 
CGGCCGGGCC GAACGGCCCC CAGCGGCCCG CGGAGGTACC CGGGCCCCGT GGCCGAGCCC 
CTGGCCGGGG AGCGGCCTCC CACGGGAGGC CACGGAAGCA GCCGCTCGCC CGCGATGGAG 
CGGCGGATCC CGGGCCCGGC CCGGAGCGAG TCCCCCAGGG CGCCGTGTCG CCACGGCGGG 
GCGCGATGGC CGGCGTCCGG CGCGCACGCG GCCGAGGGCC CCCCGGGCCC CCGGCACCAC 
CACGGTTACT ACCGGGGCTC CGACTACGAC GAGCCAGACG GCCCGGGCGG CGGCGGCGGG 
GAGGAGGAGG AGCCCCGGGC CGCCGCCTAC GACGCGCCGC CCCCCGCGCG CCACGGGTGC 
TCGCCCAGGA CTCCCCGGGC CGCGGGCCCG GCCTGCGCCT CGCCTTCCAG GCACGGCCGG 
CGACTCCCGA ACGGCTACTA CCCGGCCCAC GGACTGGCCA GGCCCCGCGG GCCGGGCTCC 
CGGAAAGGCC TGCACGAAGC CTACAGCGAG ACCGACGACG ATGACTGGTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1A ( XM_024993852.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849620.1 Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X5, mRNA.

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CloneID	OBa235774
Clone ID Related Accession (Same CDS sequence)	XM_024993852.1
Accession Version	XM_024993852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACTGGCA ATAGCAAAGG AAAGGACATC 
AACACGATTA AATCCCTCCG AGTCCTCCGG GTGCTACGAC CTCTTAAAAC CATCAAGCGG 
TTGCCAAAGC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTCAAGAA TGTCTTCAAC 
ATCCTCATCG TCTACATGCT GTTCATGTTC ATCTTCGCCG TGGTGGCCGT GCAGCTCTTC 
AAGGGCAAGT TTTTCCACTG CACAGATGAG TCCAAGGAGT TTGAGAAAGA TTGCCGCGGC 
AAGTACCTCC TCTATGAGAA GAATGAGGTA AAGGCGAGGG ATCGAGAGTG GAAGAAGTAT 
GAGTTCCATT ATGACAATGT GCTGTGGGCG CTGCTGACCC TCTTCACCGT GTCCACGGGA 
GAAGGCTGGC CACAGGTCCT CAAGCACTCC GTGGACGCCA CCTTCGAGAA CCAGGGCCCC 
AGCCCCGGGT ACCGCATGGA GATGTCCATC TTCTACGTCG TCTACTTTGT GGTGTTCCCC 
TTCTTCTTTG TCAACATCTT TGTGGCCTTG ATCATCATCA CCTTCCAGGA GCAGGGGGAC 
AAGATGATGG AGGAGTACAG CCTGGAGAAA AATGAGAGGG CGTGCATCGA CTTCGCCATC 
AGCGCCAAAC CCCTGACCCG GCACATGCCG CAGAACAAGC AGAGTTTCCA GTACCGCATG 
TGGCAGTTCG TGGTGTCCCC GCCTTTCGAG TACACCATCA TGGCCATGAT CGCCCTCAAC 
ACCATCGTGC TCATGATGAA GTTCTACGGG GCCTCTGTTG CTTATGAGAA TGCTCTGAGA 
GTATTCAACA TCGTCTTCAC CTCCCTCTTC TCACTGGAAT GTGTGCTGAA AGTCATGGCT 
TTTGGGATTC TGAATTATTT TCGCGATGCC TGGAACATCT TCGACTTTGT GACTGTTCTG 
GGCAGCATCA CCGACATCCT CGTGACCGAG TTTGGGAATA ACTTCATCAA CTTGAGCTTC 
CTCCGCCTCT TCCGAGCTGC CCGGCTCATC AAACTCCTCC GTCAGGGTTA CACCATCCGA 
ATTCTTCTCT GGACCTTTGT GCAGTCCTTC AAGGCCCTGC CCTATGTCTG TCTACTGATT 
GCCATGCTTT TTTTCATCTA CGCCATCATT GGGATGCAGG TGTTTGGCAA CATTGGCATC 
GATGTGGAGG ACGAGGACAG TGATGAAGAT GAATTCCAAA TCACTGAGCA CAATAACTTC 
CGCACCTTCT TCCAGGCCCT CATGCTTCTC TTCCGGAGTG CCACTGGGGA GGCTTGGCAC 
AACATCATGC TTTCCTGCCT CAGCGGGAAA CCCTGTGATA AGAACTCTGG CATCCTGACT 
CACGAATGCG GCAATGAATT TGCTTATTTT TACTTCGTTT CCTTCATCTT CCTCTGCTCG 
TTTCTGATGC TGAATCTTTT TGTTGCCGTC ATCATGGACA ACTTTGAGTA CCTCACCCGA 
GATTCCTCCA TCCTGGGTCC CCACCACCTG GATGAGTACG TGCGTGTCTG GGCCGAGTAT 
GACCCTGCAG CGTGGGGCCG CATGCCTTAC CCGGACATGT ATCAGATGCT GAGACACATG 
TCTCCGCCCC TGGGTCTGGG GAAGAAGTGT CCGGCCAGAG TGGCTTACAA GAGGCTCCTG 
CGGATGGATC TGCCCGTCGC TGATGACAAC ACCGTCCACT TCAACTCTAC CCTCATGGCT 
CTGATCCGCA CGGCCCTGGA CATCAAAATT GCCAAAGGTG GAGCTGACAA ACAACAAATG 
GATGCTGAGT TGCGGAAGGA GATGATGGCC ATCTGGCCCA ATCTGTCCCA GAAGACGCTG 
GACCTGCTGG TCACCCCCCA CAAGTCCACG GACCTGACAG TGGGGAAGAT CTATGCGGCC 
ATGATGATCA TGGAGTATTA CCGGCAGAGC AAGGCCAAGA AGCTTCAGGC CATGCGCGAG 
GAACAGAACC GGACACCTCT CATGTTCCAG CGCATGGAGC CCCCATCGCC AACACAGGAG 
GGGGGGCCTG GCCAGAACGC CCTCCCCTCC ACCCAGCTGG ACCCAGGAGG AGGCTTGATG 
GCTCACGAAA GTGGCATGAA AGAGAGCCCA TCCTGGGTGA CCCAGCGGGC CCAGGAGATG 
TTCCAGAAGA CGGGCACATG GAGCCCAGAG CGGGGCCCCC CCACTGATAT GCCTAATAGC 
CAACCCAACT CTCAGTCTGT GGAGATGCGA GAGATGGGCA GAGACGGCTA CTCTGACAGT 
GAGCACTACT TCCCCGTTGA AGGCCAGGCC CGGGCCGCCT CCATGCCCCG CCTGCCTGCC 
GAGAACCAGA GGAGAAGGGG CCGGCCACGT GGGAATAACC TCAGTACCAT CTCAGACACC 
AGCCCCATGA AGCGCTCGGC CTCCGTGCTG GGCCCCAAGG CCCGGCGCCT GGACGACTAC 
TCCCTGGAGA GGGTGCCGCC CGAGGAGAAC CAGCGGCACC ACCAGAGGCG CAGAGACCGT 
GGCCACCGCA CCTCCGAGCG CTCCTTGGGC CGCTACACGG ACGTGGACAC AGGCTTGGGG 
ACAGACTTGA GCATGACCAC CCAGTCTGGG GACCTGCCAT CAAAGGAAAG AGACCAGGAG 
CGGGGCCGGC CCAAGGACCG GAAGCATCGG CAACACCACC ACCACCACCA CCACCACCAT 
CCCCCGCCCC CTGACAAGGA GCGCTACACC CAGGAGCGGC CTGAGCACGG CCGGCCCCGG 
GCCCGTGACC AGCGCTGGTC CCGCTCACCC AGTGAGGGCC GGGAGCACAT GGCCCACCGT 
CAGGGCAGTA GTTCCGTAAG TGGAAGCCCA GCCCCCTCAA CATCTGGTAC CAGCACTCCG 
CGGCGGGGAC GCCGCCAGCT CCCCCAGACC CCCTCCACTC CCCGGCCGCA CGTGTCCTAT 
TCCCCCGTGA TCCGTAAGGC CGGCGGCTCG GGTCCCCAGC AGCAGCAGCA GCCCGCGGCG 
CGGCCGGGCC GAACGGCCCC CAGCGGCCCG CGGAGGTACC CGGGCCCCGT GGCCGAGCCC 
CTGGCCGGGG AGCGGCCTCC CACGGGAGGC CACGGAAGCA GCCGCTCGCC CGCGATGGAG 
CGGCGGATCC CGGGCCCGGC CCGGAGCGAG TCCCCCAGGG CGCCGTGTCG CCACGGCGGG 
GCGCGATGGC CGGCGTCCGG CGCGCACGCG GCCGAGGGCC CCCCGGGCCC CCGGCACCAC 
CACGGTTACT ACCGGGGCTC CGACTACGAC GAGCCAGACG GCCCGGGCGG CGGCGGCGGG 
GAGGAGGAGG AGCCCCGGGC CGCCGCCTAC GACGCGCCGC CCCCCGCGCG CCACGGGTGC 
TCGCCCAGGA CTCCCCGGGC CGCGGGCCCG GCCTGCGCCT CGCCTTCCAG GCACGGCCGG 
CGACTCCCGA ACGGCTACTA CCCGGCCCAC GGACTGGCCA GGCCCCGCGG GCCGGGCTCC 
CGGAAAGGCC TGCACGAAGC CTACAGCGAG ACCGACGACG ATGACTGGTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849620.1
CDS	230..7723
Translation	MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRHPFDGALRRATIKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSSSVGLDTRRPWAGSQEAELSREGPYGRESDHHAREGGLEQPGFWEGEAERGKAGDPHRRHAHRQAGSRESRSGSPRTGADGEPRRHRAHRRPGDEAPDDKADRRSRHREGSRPARGGEGESEGPDGGGERRRRHRHGPPPAYDADMRREDKERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESASPHDSLSHAGLPQSPAKMGNSMDPGPTPAPTTTATNPQNAVSRRTANNPGNPSNPGPPKTPENSLIVTNPSSTQANSAKTARKPDHTTVDIPPACPPPLNHTIIQVNKNANPDPLPKKEEEKKEEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTHECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMPYPDMYQMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPGQNALPSTQLDPGGGLMAHESGMKESPSWVTQRAQEMFQKTGTWSPERGPPTDMPNSQPNSQSVEMREMGRDGYSDSEHYFPVEGQARAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRPKDRKHRQHHHHHHHHHPPPPDKERYTQERPEHGRPRARDQRWSRSPSEGREHMAHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPSTPRPHVSYSPVIRKAGGSGPQQQQQPAARPGRTAPSGPRRYPGPVAEPLAGERPPTGGHGSSRSPAMERRIPGPARSESPRAPCRHGGARWPASGAHAAEGPPGPRHHHGYYRGSDYDEPDGPGGGGGEEEEPRAAAYDAPPPARHGCSPRTPRAAGPACASPSRHGRRLPNGYYPAHGLARPRGPGSRKGLHEAYSETDDDDWC

Target ORF information:

RefSeq Version	XM_024993852.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993852.1

ORF Insert Sequence:

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACTGGCA ATAGCAAAGG AAAGGACATC 
AACACGATTA AATCCCTCCG AGTCCTCCGG GTGCTACGAC CTCTTAAAAC CATCAAGCGG 
TTGCCAAAGC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTCAAGAA TGTCTTCAAC 
ATCCTCATCG TCTACATGCT GTTCATGTTC ATCTTCGCCG TGGTGGCCGT GCAGCTCTTC 
AAGGGCAAGT TTTTCCACTG CACAGATGAG TCCAAGGAGT TTGAGAAAGA TTGCCGCGGC 
AAGTACCTCC TCTATGAGAA GAATGAGGTA AAGGCGAGGG ATCGAGAGTG GAAGAAGTAT 
GAGTTCCATT ATGACAATGT GCTGTGGGCG CTGCTGACCC TCTTCACCGT GTCCACGGGA 
GAAGGCTGGC CACAGGTCCT CAAGCACTCC GTGGACGCCA CCTTCGAGAA CCAGGGCCCC 
AGCCCCGGGT ACCGCATGGA GATGTCCATC TTCTACGTCG TCTACTTTGT GGTGTTCCCC 
TTCTTCTTTG TCAACATCTT TGTGGCCTTG ATCATCATCA CCTTCCAGGA GCAGGGGGAC 
AAGATGATGG AGGAGTACAG CCTGGAGAAA AATGAGAGGG CGTGCATCGA CTTCGCCATC 
AGCGCCAAAC CCCTGACCCG GCACATGCCG CAGAACAAGC AGAGTTTCCA GTACCGCATG 
TGGCAGTTCG TGGTGTCCCC GCCTTTCGAG TACACCATCA TGGCCATGAT CGCCCTCAAC 
ACCATCGTGC TCATGATGAA GTTCTACGGG GCCTCTGTTG CTTATGAGAA TGCTCTGAGA 
GTATTCAACA TCGTCTTCAC CTCCCTCTTC TCACTGGAAT GTGTGCTGAA AGTCATGGCT 
TTTGGGATTC TGAATTATTT TCGCGATGCC TGGAACATCT TCGACTTTGT GACTGTTCTG 
GGCAGCATCA CCGACATCCT CGTGACCGAG TTTGGGAATA ACTTCATCAA CTTGAGCTTC 
CTCCGCCTCT TCCGAGCTGC CCGGCTCATC AAACTCCTCC GTCAGGGTTA CACCATCCGA 
ATTCTTCTCT GGACCTTTGT GCAGTCCTTC AAGGCCCTGC CCTATGTCTG TCTACTGATT 
GCCATGCTTT TTTTCATCTA CGCCATCATT GGGATGCAGG TGTTTGGCAA CATTGGCATC 
GATGTGGAGG ACGAGGACAG TGATGAAGAT GAATTCCAAA TCACTGAGCA CAATAACTTC 
CGCACCTTCT TCCAGGCCCT CATGCTTCTC TTCCGGAGTG CCACTGGGGA GGCTTGGCAC 
AACATCATGC TTTCCTGCCT CAGCGGGAAA CCCTGTGATA AGAACTCTGG CATCCTGACT 
CACGAATGCG GCAATGAATT TGCTTATTTT TACTTCGTTT CCTTCATCTT CCTCTGCTCG 
TTTCTGATGC TGAATCTTTT TGTTGCCGTC ATCATGGACA ACTTTGAGTA CCTCACCCGA 
GATTCCTCCA TCCTGGGTCC CCACCACCTG GATGAGTACG TGCGTGTCTG GGCCGAGTAT 
GACCCTGCAG CGTGGGGCCG CATGCCTTAC CCGGACATGT ATCAGATGCT GAGACACATG 
TCTCCGCCCC TGGGTCTGGG GAAGAAGTGT CCGGCCAGAG TGGCTTACAA GAGGCTCCTG 
CGGATGGATC TGCCCGTCGC TGATGACAAC ACCGTCCACT TCAACTCTAC CCTCATGGCT 
CTGATCCGCA CGGCCCTGGA CATCAAAATT GCCAAAGGTG GAGCTGACAA ACAACAAATG 
GATGCTGAGT TGCGGAAGGA GATGATGGCC ATCTGGCCCA ATCTGTCCCA GAAGACGCTG 
GACCTGCTGG TCACCCCCCA CAAGTCCACG GACCTGACAG TGGGGAAGAT CTATGCGGCC 
ATGATGATCA TGGAGTATTA CCGGCAGAGC AAGGCCAAGA AGCTTCAGGC CATGCGCGAG 
GAACAGAACC GGACACCTCT CATGTTCCAG CGCATGGAGC CCCCATCGCC AACACAGGAG 
GGGGGGCCTG GCCAGAACGC CCTCCCCTCC ACCCAGCTGG ACCCAGGAGG AGGCTTGATG 
GCTCACGAAA GTGGCATGAA AGAGAGCCCA TCCTGGGTGA CCCAGCGGGC CCAGGAGATG 
TTCCAGAAGA CGGGCACATG GAGCCCAGAG CGGGGCCCCC CCACTGATAT GCCTAATAGC 
CAACCCAACT CTCAGTCTGT GGAGATGCGA GAGATGGGCA GAGACGGCTA CTCTGACAGT 
GAGCACTACT TCCCCGTTGA AGGCCAGGCC CGGGCCGCCT CCATGCCCCG CCTGCCTGCC 
GAGAACCAGA GGAGAAGGGG CCGGCCACGT GGGAATAACC TCAGTACCAT CTCAGACACC 
AGCCCCATGA AGCGCTCGGC CTCCGTGCTG GGCCCCAAGG CCCGGCGCCT GGACGACTAC 
TCCCTGGAGA GGGTGCCGCC CGAGGAGAAC CAGCGGCACC ACCAGAGGCG CAGAGACCGT 
GGCCACCGCA CCTCCGAGCG CTCCTTGGGC CGCTACACGG ACGTGGACAC AGGCTTGGGG 
ACAGACTTGA GCATGACCAC CCAGTCTGGG GACCTGCCAT CAAAGGAAAG AGACCAGGAG 
CGGGGCCGGC CCAAGGACCG GAAGCATCGG CAACACCACC ACCACCACCA CCACCACCAT 
CCCCCGCCCC CTGACAAGGA GCGCTACACC CAGGAGCGGC CTGAGCACGG CCGGCCCCGG 
GCCCGTGACC AGCGCTGGTC CCGCTCACCC AGTGAGGGCC GGGAGCACAT GGCCCACCGT 
CAGGGCAGTA GTTCCGTAAG TGGAAGCCCA GCCCCCTCAA CATCTGGTAC CAGCACTCCG 
CGGCGGGGAC GCCGCCAGCT CCCCCAGACC CCCTCCACTC CCCGGCCGCA CGTGTCCTAT 
TCCCCCGTGA TCCGTAAGGC CGGCGGCTCG GGTCCCCAGC AGCAGCAGCA GCCCGCGGCG 
CGGCCGGGCC GAACGGCCCC CAGCGGCCCG CGGAGGTACC CGGGCCCCGT GGCCGAGCCC 
CTGGCCGGGG AGCGGCCTCC CACGGGAGGC CACGGAAGCA GCCGCTCGCC CGCGATGGAG 
CGGCGGATCC CGGGCCCGGC CCGGAGCGAG TCCCCCAGGG CGCCGTGTCG CCACGGCGGG 
GCGCGATGGC CGGCGTCCGG CGCGCACGCG GCCGAGGGCC CCCCGGGCCC CCGGCACCAC 
CACGGTTACT ACCGGGGCTC CGACTACGAC GAGCCAGACG GCCCGGGCGG CGGCGGCGGG 
GAGGAGGAGG AGCCCCGGGC CGCCGCCTAC GACGCGCCGC CCCCCGCGCG CCACGGGTGC 
TCGCCCAGGA CTCCCCGGGC CGCGGGCCCG GCCTGCGCCT CGCCTTCCAG GCACGGCCGG 
CGACTCCCGA ACGGCTACTA CCCGGCCCAC GGACTGGCCA GGCCCCGCGG GCCGGGCTCC 
CGGAAAGGCC TGCACGAAGC CTACAGCGAG ACCGACGACG ATGACTGGTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1A ( XM_024993853.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849621.1 Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X6, mRNA.

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CloneID	OBa235775
Clone ID Related Accession (Same CDS sequence)	XM_024993853.1
Accession Version	XM_024993853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGCTCTG 
CGGAGAGCCA CTATAAAGAA GAGCAAGACA GACCTGCTCA ACCCCGAGGA GGCTGAGGAT 
CAGCTGGCCG ACATCGCCTC TGTGGGGTCT CCCTTCGCCC GAGCCAGCAT TAAAAGTGCC 
AAGCTGGAGA ACTCAACTTT TTTTCACAAA AAGGAGAGGA GGATGCGTTT CTACATCCGC 
CGCATGGTCA AAACTCAGGC CTTCTACTGG ACTGTGCTCA GTCTGGTAGC CCTCAACACG 
CTGTGTGTCG CTATCGTTCA CTACAACCAG CCTGAGTGGC TCTCCGACTT CCTCTACTAT 
GCAGAATTCA TTTTCTTAGG ACTCTTTATG TCCGAAATGT TTATAAAAAT GTACGGGCTT 
GGGACGCGGC CTTACTTCCA CTCTTCCTTC AACTGTTTTG ATTGTGGGGT CATCATCGGG 
AGTATCTTTG AGGTCATCTG GGCTGTCATA AAACCCGGCA CATCCTTTGG AATCAGTGTG 
TTACGAGCCC TCAGGTTATT GCGTATTTTC AAAGTCACAA AGTACTGGGC ATCTCTCAGG 
AACCTGGTCG TCTCTCTTCT CAACTCCATG AAATCCATCA TCAGCCTGTT GTTCCTCCTT 
TTCCTATTCA TCGTCGTCTT TGCCCTTCTG GGAATGCAAC TCTTTGGCGG CCAGTTTAAT 
TTCGATGAAG GGACTCCTCC TACCAACTTC GACACTTTTC CAGCAGCAAT AATGACAGTT 
TTTCAGATCC TGACGGGCGA AGACTGGAAC GAGGTCATGT ATGACGGCAT CAAGTCTCAG 
GGGGGCGTGC AAGGCGGCAT GGTGTTCTCC ATCTACTTCA TCGTGCTGAC GCTCTTCGGG 
AACTACACCC TCCTGAACGT GTTCTTGGCC ATCGCGGTGG ATAATCTGGC TAACGCCCAG 
GAGCTCACCA AGGATGAACA AGAAGAAGAA GAGGCTGCCA ACCAGAAACT CGCCCTCCAG 
AAAGCCAAAG AGGTGGCAGA AGTGAGTCCT CTGTCTGCAG CCAACATGTC CATAGCTGTG 
AAGGAGCAAC AGAAGAACCA GAAGCCCGCC AAGTCGGTGT GGGAGCAGCG GACAAGCGAG 
ATGAGGAAGC AGAATCTTCT GGCCAGTCGG GAGGCACTCT ACAACGAGAT GGACCCAGAC 
GAGCGCTGGA AGGCCTCCTA CGCTCGCCAC CTGCGGCCCG ACATGAAGAC CCACCTGGAC 
CGGCCGCTGG TGGTCGACCC CCAGGAGAAC CGTAACAACA ACACCAACAA GAGCCGGGCG 
GCCGAGCCCA CGGTGGACCA GCGCCTCGGG CAGCAGCGAG CCGAGGACTT CCTCAGGAAA 
CAGGCCCGCT ACCACGACCG GGCCCGGGAC CCCAGCAGCT CCGTGGGCCT GGACACCCGG 
AGGCCCTGGG CGGGCAGCCA GGAAGCCGAG CTGAGCCGGG AGGGGCCTTA CGGTCGCGAG 
TCGGACCACC ACGCCCGGGA GGGCGGCCTG GAGCAGCCGG GGTTCTGGGA GGGCGAGGCG 
GAGCGGGGCA AGGCGGGCGA CCCGCACCGG AGGCACGCGC ACCGGCAGGC GGGCAGCCGC 
GAGAGCCGCA GCGGGTCCCC GCGCACCGGG GCCGACGGAG AGCCCCGCCG GCACCGGGCG 
CACCGCAGGC CGGGCGACGA GGCCCCGGAC GACAAGGCCG ATCGGAGGTC CCGGCACCGC 
GAGGGCAGCC GGCCGGCCCG GGGCGGCGAG GGCGAGAGCG AGGGCCCAGA CGGCGGCGGC 
GAGCGGAGGC GGCGGCACCG GCATGGCCCT CCCCCAGCGT ACGACGCGGA CATGCGCAGG 
GAGGACAAGG AGCGCAGGCA CCGCAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCCGTG 
TCAGGCCCCA ACCTGTCGAC CACACGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCC 
CCGCTGGCCG AGGATATCGA CAACATGAAG AACAACAAAC TGGCCACTGC GGAGTCAGCC 
AGTCCCCACG ACAGCCTCAG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGGAAC 
AGCATGGACC CTGGCCCCAC ACCGGCCCCC ACCACCACGG CCACCAACCC TCAGAATGCC 
GTCAGTCGCC GGACAGCCAA CAACCCGGGG AACCCGTCCA ACCCCGGCCC CCCCAAGACC 
CCTGAGAACA GCCTTATCGT CACCAACCCC AGCAGCACCC AGGCCAACTC AGCTAAGACT 
GCCAGGAAAC CTGACCACAC CACGGTGGAC ATCCCCCCAG CCTGCCCACC CCCACTCAAC 
CACACCATCA TCCAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAGGAGGAA 
GAGAAGAAAG AGGAGGAGGA AGACGACCCC GGGGAAGACG GCCCCAAGCC CATGCCCCCC 
TACAGCTCCA TGTTTATCCT CTCTACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC 
CTGAACCTGC GCTACTTTGA GATGTGCATT CTCATGGTCA TCGCCATGAG CAGCATCGCC 
CTGGCGGCCG AGGATCCTGT TCAGCCCAAC GCACCACGAA ACAATGTGCT GCGATATTTT 
GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT CGACCTGGGG 
CTCGTCCTGC ACCAGGGTGC CTACTTCCGT GACCTCTGGA ACATTCTCGA CTTCATAGTG 
GTCAGTGGGG CCCTGGTGGC CTTTGCCTTC ACTGGCAATA GCAAAGGAAA GGACATCAAC 
ACGATTAAAT CCCTCCGAGT CCTCCGGGTG CTACGACCTC TTAAAACCAT CAAGCGGTTG 
CCAAAGCTCA AGGCCGTGTT TGACTGCGTG GTGAACTCTC TCAAGAATGT CTTCAACATC 
CTCATCGTCT ACATGCTGTT CATGTTCATC TTCGCCGTGG TGGCCGTGCA GCTCTTCAAG 
GGCAAGTTTT TCCACTGCAC AGATGAGTCC AAGGAGTTTG AGAAAGATTG CCGCGGCAAG 
TACCTCCTCT ATGAGAAGAA TGAGGTAAAG GCGAGGGATC GAGAGTGGAA GAAGTATGAG 
TTCCATTATG ACAATGTGCT GTGGGCGCTG CTGACCCTCT TCACCGTGTC CACGGGAGAA 
GGCTGGCCAC AGGTCCTCAA GCACTCCGTG GACGCCACCT TCGAGAACCA GGGCCCCAGC 
CCCGGGTACC GCATGGAGAT GTCCATCTTC TACGTCGTCT ACTTTGTGGT GTTCCCCTTC 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATCATCACCT TCCAGGAGCA GGGGGACAAG 
ATGATGGAGG AGTACAGCCT GGAGAAAAAT GAGAGGGCGT GCATCGACTT CGCCATCAGC 
GCCAAACCCC TGACCCGGCA CATGCCGCAG AACAAGCAGA GTTTCCAGTA CCGCATGTGG 
CAGTTCGTGG TGTCCCCGCC TTTCGAGTAC ACCATCATGG CCATGATCGC CCTCAACACC 
ATCGTGCTCA TGATGAAGTT CTACGGGGCC TCTGTTGCTT ATGAGAATGC TCTGAGAGTA 
TTCAACATCG TCTTCACCTC CCTCTTCTCA CTGGAATGTG TGCTGAAAGT CATGGCTTTT 
GGGATTCTGA ATTATTTTCG CGATGCCTGG AACATCTTCG ACTTTGTGAC TGTTCTGGGC 
AGCATCACCG ACATCCTCGT GACCGAGTTT GGGAATAACT TCATCAACTT GAGCTTCCTC 
CGCCTCTTCC GAGCTGCCCG GCTCATCAAA CTCCTCCGTC AGGGTTACAC CATCCGAATT 
CTTCTCTGGA CCTTTGTGCA GTCCTTCAAG GCCCTGCCCT ATGTCTGTCT ACTGATTGCC 
ATGCTTTTTT TCATCTACGC CATCATTGGG ATGCAGGTGT TTGGCAACAT TGGCATCGAT 
GTGGAGGACG AGGACAGTGA TGAAGATGAA TTCCAAATCA CTGAGCACAA TAACTTCCGC 
ACCTTCTTCC AGGCCCTCAT GCTTCTCTTC CGGAGTGCCA CTGGGGAGGC TTGGCACAAC 
ATCATGCTTT CCTGCCTCAG CGGGAAACCC TGTGATAAGA ACTCTGGCAT CCTGACTCAC 
GAATGCGGCA ATGAATTTGC TTATTTTTAC TTCGTTTCCT TCATCTTCCT CTGCTCGTTT 
CTGATGCTGA ATCTTTTTGT TGCCGTCATC ATGGACAACT TTGAGTACCT CACCCGAGAT 
TCCTCCATCC TGGGTCCCCA CCACCTGGAT GAGTACGTGC GTGTCTGGGC CGAGTATGAC 
CCTGCAGCGT GGGGCCGCAT GCCTTACCCG GACATGTATC AGATGCTGAG ACACATGTCT 
CCGCCCCTGG GTCTGGGGAA GAAGTGTCCG GCCAGAGTGG CTTACAAGAG GCTCCTGCGG 
ATGGATCTGC CCGTCGCTGA TGACAACACC GTCCACTTCA ACTCTACCCT CATGGCTCTG 
ATCCGCACGG CCCTGGACAT CAAAATTGCC AAAGGTGGAG CTGACAAACA ACAAATGGAT 
GCTGAGTTGC GGAAGGAGAT GATGGCCATC TGGCCCAATC TGTCCCAGAA GACGCTGGAC 
CTGCTGGTCA CCCCCCACAA GTCCACGGAC CTGACAGTGG GGAAGATCTA TGCGGCCATG 
ATGATCATGG AGTATTACCG GCAGAGCAAG GCCAAGAAGC TTCAGGCCAT GCGCGAGGAA 
CAGAACCGGA CACCTCTCAT GTTCCAGCGC ATGGAGCCCC CATCGCCAAC ACAGGAGGGG 
GGGCCTGGCC AGAACGCCCT CCCCTCCACC CAGCTGGACC CAGGAGGAGG CTTGATGGCT 
CACGAAAGTG GCATGAAAGA GAGCCCATCC TGGGTGACCC AGCGGGCCCA GGAGATGTTC 
CAGAAGACGG GCACATGGAG CCCAGAGCGG GGCCCCCCCA CTGATATGCC TAATAGCCAA 
CCCAACTCTC AGTCTGTGGA GATGCGAGAG ATGGGCAGAG ACGGCTACTC TGACAGTGAG 
CACTACTTCC CCGTTGAAGG CCAGGCCCGG GCCGCCTCCA TGCCCCGCCT GCCTGCCGAG 
AACCAGAGGA GAAGGGGCCG GCCACGTGGG AATAACCTCA GTACCATCTC AGACACCAGC 
CCCATGAAGC GCTCGGCCTC CGTGCTGGGC CCCAAGGCCC GGCGCCTGGA CGACTACTCC 
CTGGAGAGGG TGCCGCCCGA GGAGAACCAG CGGCACCACC AGAGGCGCAG AGACCGTGGC 
CACCGCACCT CCGAGCGCTC CTTGGGCCGC TACACGGACG TGGACACAGG CTTGGGGACA 
GACTTGAGCA TGACCACCCA GTCTGGGGAC CTGCCATCAA AGGAAAGAGA CCAGGAGCGG 
GGCCGGCCCA AGGACCGGAA GCATCGGCAA CACCACCACC ACCACCACCA CCACCATCCC 
CCGCCCCCTG ACAAGGAGCG CTACACCCAG GAGCGGCCTG AGCACGGCCG GCCCCGGGCC 
CGTGACCAGC GCTGGTCCCG CTCACCCAGT GAGGGCCGGG AGCACATGGC CCACCGTCAG 
GGCAGTAGTT CCGTAAGTGG AAGCCCAGCC CCCTCAACAT CTGGTACCAG CACTCCGCGG 
CGGGGACGCC GCCAGCTCCC CCAGACCCCC TCCACTCCCC GGCCGCACGT GTCCTATTCC 
CCCGTGATCC GTAAGGCCGG CGGCTCGGGT CCCCAGCAGC AGCAGCAGCC CGCGGCGCGG 
CCGGGCCGAA CGGCCCCCAG CGGCCCGCGG AGGTACCCGG GCCCCGTGGC CGAGCCCCTG 
GCCGGGGAGC GGCCTCCCAC GGGAGGCCAC GGAAGCAGCC GCTCGCCCGC GATGGAGCGG 
CGGATCCCGG GCCCGGCCCG GAGCGAGTCC CCCAGGGCGC CGTGTCGCCA CGGCGGGGCG 
CGATGGCCGG CGTCCGGCGC GCACGCGGCC GAGGGCCCCC CGGGCCCCCG GCACCACCAC 
GGTTACTACC GGGGCTCCGA CTACGACGAG CCAGACGGCC CGGGCGGCGG CGGCGGGGAG 
GAGGAGGAGC CCCGGGCCGC CGCCTACGAC GCGCCGCCCC CCGCGCGCCA CGGGTGCTCG 
CCCAGGACTC CCCGGGCCGC GGGCCCGGCC TGCGCCTCGC CTTCCAGGCA CGGCCGGCGA 
CTCCCGAACG GCTACTACCC GGCCCACGGA CTGGCCAGGC CCCGCGGGCC GGGCTCCCGG 
AAAGGCCTGC ACGAAGCCTA CAGCGAGACC GACGACGATG ACTGGTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849621.1
CDS	230..7720
Translation	MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRHPFDALRRATIKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSSSVGLDTRRPWAGSQEAELSREGPYGRESDHHAREGGLEQPGFWEGEAERGKAGDPHRRHAHRQAGSRESRSGSPRTGADGEPRRHRAHRRPGDEAPDDKADRRSRHREGSRPARGGEGESEGPDGGGERRRRHRHGPPPAYDADMRREDKERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESASPHDSLSHAGLPQSPAKMGNSMDPGPTPAPTTTATNPQNAVSRRTANNPGNPSNPGPPKTPENSLIVTNPSSTQANSAKTARKPDHTTVDIPPACPPPLNHTIIQVNKNANPDPLPKKEEEKKEEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTHECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMPYPDMYQMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPGQNALPSTQLDPGGGLMAHESGMKESPSWVTQRAQEMFQKTGTWSPERGPPTDMPNSQPNSQSVEMREMGRDGYSDSEHYFPVEGQARAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRPKDRKHRQHHHHHHHHHPPPPDKERYTQERPEHGRPRARDQRWSRSPSEGREHMAHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPSTPRPHVSYSPVIRKAGGSGPQQQQQPAARPGRTAPSGPRRYPGPVAEPLAGERPPTGGHGSSRSPAMERRIPGPARSESPRAPCRHGGARWPASGAHAAEGPPGPRHHHGYYRGSDYDEPDGPGGGGGEEEEPRAAAYDAPPPARHGCSPRTPRAAGPACASPSRHGRRLPNGYYPAHGLARPRGPGSRKGLHEAYSETDDDDWC

Target ORF information:

RefSeq Version	XM_024993853.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993853.1

ORF Insert Sequence:

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGCTCTG 
CGGAGAGCCA CTATAAAGAA GAGCAAGACA GACCTGCTCA ACCCCGAGGA GGCTGAGGAT 
CAGCTGGCCG ACATCGCCTC TGTGGGGTCT CCCTTCGCCC GAGCCAGCAT TAAAAGTGCC 
AAGCTGGAGA ACTCAACTTT TTTTCACAAA AAGGAGAGGA GGATGCGTTT CTACATCCGC 
CGCATGGTCA AAACTCAGGC CTTCTACTGG ACTGTGCTCA GTCTGGTAGC CCTCAACACG 
CTGTGTGTCG CTATCGTTCA CTACAACCAG CCTGAGTGGC TCTCCGACTT CCTCTACTAT 
GCAGAATTCA TTTTCTTAGG ACTCTTTATG TCCGAAATGT TTATAAAAAT GTACGGGCTT 
GGGACGCGGC CTTACTTCCA CTCTTCCTTC AACTGTTTTG ATTGTGGGGT CATCATCGGG 
AGTATCTTTG AGGTCATCTG GGCTGTCATA AAACCCGGCA CATCCTTTGG AATCAGTGTG 
TTACGAGCCC TCAGGTTATT GCGTATTTTC AAAGTCACAA AGTACTGGGC ATCTCTCAGG 
AACCTGGTCG TCTCTCTTCT CAACTCCATG AAATCCATCA TCAGCCTGTT GTTCCTCCTT 
TTCCTATTCA TCGTCGTCTT TGCCCTTCTG GGAATGCAAC TCTTTGGCGG CCAGTTTAAT 
TTCGATGAAG GGACTCCTCC TACCAACTTC GACACTTTTC CAGCAGCAAT AATGACAGTT 
TTTCAGATCC TGACGGGCGA AGACTGGAAC GAGGTCATGT ATGACGGCAT CAAGTCTCAG 
GGGGGCGTGC AAGGCGGCAT GGTGTTCTCC ATCTACTTCA TCGTGCTGAC GCTCTTCGGG 
AACTACACCC TCCTGAACGT GTTCTTGGCC ATCGCGGTGG ATAATCTGGC TAACGCCCAG 
GAGCTCACCA AGGATGAACA AGAAGAAGAA GAGGCTGCCA ACCAGAAACT CGCCCTCCAG 
AAAGCCAAAG AGGTGGCAGA AGTGAGTCCT CTGTCTGCAG CCAACATGTC CATAGCTGTG 
AAGGAGCAAC AGAAGAACCA GAAGCCCGCC AAGTCGGTGT GGGAGCAGCG GACAAGCGAG 
ATGAGGAAGC AGAATCTTCT GGCCAGTCGG GAGGCACTCT ACAACGAGAT GGACCCAGAC 
GAGCGCTGGA AGGCCTCCTA CGCTCGCCAC CTGCGGCCCG ACATGAAGAC CCACCTGGAC 
CGGCCGCTGG TGGTCGACCC CCAGGAGAAC CGTAACAACA ACACCAACAA GAGCCGGGCG 
GCCGAGCCCA CGGTGGACCA GCGCCTCGGG CAGCAGCGAG CCGAGGACTT CCTCAGGAAA 
CAGGCCCGCT ACCACGACCG GGCCCGGGAC CCCAGCAGCT CCGTGGGCCT GGACACCCGG 
AGGCCCTGGG CGGGCAGCCA GGAAGCCGAG CTGAGCCGGG AGGGGCCTTA CGGTCGCGAG 
TCGGACCACC ACGCCCGGGA GGGCGGCCTG GAGCAGCCGG GGTTCTGGGA GGGCGAGGCG 
GAGCGGGGCA AGGCGGGCGA CCCGCACCGG AGGCACGCGC ACCGGCAGGC GGGCAGCCGC 
GAGAGCCGCA GCGGGTCCCC GCGCACCGGG GCCGACGGAG AGCCCCGCCG GCACCGGGCG 
CACCGCAGGC CGGGCGACGA GGCCCCGGAC GACAAGGCCG ATCGGAGGTC CCGGCACCGC 
GAGGGCAGCC GGCCGGCCCG GGGCGGCGAG GGCGAGAGCG AGGGCCCAGA CGGCGGCGGC 
GAGCGGAGGC GGCGGCACCG GCATGGCCCT CCCCCAGCGT ACGACGCGGA CATGCGCAGG 
GAGGACAAGG AGCGCAGGCA CCGCAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCCGTG 
TCAGGCCCCA ACCTGTCGAC CACACGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCC 
CCGCTGGCCG AGGATATCGA CAACATGAAG AACAACAAAC TGGCCACTGC GGAGTCAGCC 
AGTCCCCACG ACAGCCTCAG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGGAAC 
AGCATGGACC CTGGCCCCAC ACCGGCCCCC ACCACCACGG CCACCAACCC TCAGAATGCC 
GTCAGTCGCC GGACAGCCAA CAACCCGGGG AACCCGTCCA ACCCCGGCCC CCCCAAGACC 
CCTGAGAACA GCCTTATCGT CACCAACCCC AGCAGCACCC AGGCCAACTC AGCTAAGACT 
GCCAGGAAAC CTGACCACAC CACGGTGGAC ATCCCCCCAG CCTGCCCACC CCCACTCAAC 
CACACCATCA TCCAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAGGAGGAA 
GAGAAGAAAG AGGAGGAGGA AGACGACCCC GGGGAAGACG GCCCCAAGCC CATGCCCCCC 
TACAGCTCCA TGTTTATCCT CTCTACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC 
CTGAACCTGC GCTACTTTGA GATGTGCATT CTCATGGTCA TCGCCATGAG CAGCATCGCC 
CTGGCGGCCG AGGATCCTGT TCAGCCCAAC GCACCACGAA ACAATGTGCT GCGATATTTT 
GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT CGACCTGGGG 
CTCGTCCTGC ACCAGGGTGC CTACTTCCGT GACCTCTGGA ACATTCTCGA CTTCATAGTG 
GTCAGTGGGG CCCTGGTGGC CTTTGCCTTC ACTGGCAATA GCAAAGGAAA GGACATCAAC 
ACGATTAAAT CCCTCCGAGT CCTCCGGGTG CTACGACCTC TTAAAACCAT CAAGCGGTTG 
CCAAAGCTCA AGGCCGTGTT TGACTGCGTG GTGAACTCTC TCAAGAATGT CTTCAACATC 
CTCATCGTCT ACATGCTGTT CATGTTCATC TTCGCCGTGG TGGCCGTGCA GCTCTTCAAG 
GGCAAGTTTT TCCACTGCAC AGATGAGTCC AAGGAGTTTG AGAAAGATTG CCGCGGCAAG 
TACCTCCTCT ATGAGAAGAA TGAGGTAAAG GCGAGGGATC GAGAGTGGAA GAAGTATGAG 
TTCCATTATG ACAATGTGCT GTGGGCGCTG CTGACCCTCT TCACCGTGTC CACGGGAGAA 
GGCTGGCCAC AGGTCCTCAA GCACTCCGTG GACGCCACCT TCGAGAACCA GGGCCCCAGC 
CCCGGGTACC GCATGGAGAT GTCCATCTTC TACGTCGTCT ACTTTGTGGT GTTCCCCTTC 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATCATCACCT TCCAGGAGCA GGGGGACAAG 
ATGATGGAGG AGTACAGCCT GGAGAAAAAT GAGAGGGCGT GCATCGACTT CGCCATCAGC 
GCCAAACCCC TGACCCGGCA CATGCCGCAG AACAAGCAGA GTTTCCAGTA CCGCATGTGG 
CAGTTCGTGG TGTCCCCGCC TTTCGAGTAC ACCATCATGG CCATGATCGC CCTCAACACC 
ATCGTGCTCA TGATGAAGTT CTACGGGGCC TCTGTTGCTT ATGAGAATGC TCTGAGAGTA 
TTCAACATCG TCTTCACCTC CCTCTTCTCA CTGGAATGTG TGCTGAAAGT CATGGCTTTT 
GGGATTCTGA ATTATTTTCG CGATGCCTGG AACATCTTCG ACTTTGTGAC TGTTCTGGGC 
AGCATCACCG ACATCCTCGT GACCGAGTTT GGGAATAACT TCATCAACTT GAGCTTCCTC 
CGCCTCTTCC GAGCTGCCCG GCTCATCAAA CTCCTCCGTC AGGGTTACAC CATCCGAATT 
CTTCTCTGGA CCTTTGTGCA GTCCTTCAAG GCCCTGCCCT ATGTCTGTCT ACTGATTGCC 
ATGCTTTTTT TCATCTACGC CATCATTGGG ATGCAGGTGT TTGGCAACAT TGGCATCGAT 
GTGGAGGACG AGGACAGTGA TGAAGATGAA TTCCAAATCA CTGAGCACAA TAACTTCCGC 
ACCTTCTTCC AGGCCCTCAT GCTTCTCTTC CGGAGTGCCA CTGGGGAGGC TTGGCACAAC 
ATCATGCTTT CCTGCCTCAG CGGGAAACCC TGTGATAAGA ACTCTGGCAT CCTGACTCAC 
GAATGCGGCA ATGAATTTGC TTATTTTTAC TTCGTTTCCT TCATCTTCCT CTGCTCGTTT 
CTGATGCTGA ATCTTTTTGT TGCCGTCATC ATGGACAACT TTGAGTACCT CACCCGAGAT 
TCCTCCATCC TGGGTCCCCA CCACCTGGAT GAGTACGTGC GTGTCTGGGC CGAGTATGAC 
CCTGCAGCGT GGGGCCGCAT GCCTTACCCG GACATGTATC AGATGCTGAG ACACATGTCT 
CCGCCCCTGG GTCTGGGGAA GAAGTGTCCG GCCAGAGTGG CTTACAAGAG GCTCCTGCGG 
ATGGATCTGC CCGTCGCTGA TGACAACACC GTCCACTTCA ACTCTACCCT CATGGCTCTG 
ATCCGCACGG CCCTGGACAT CAAAATTGCC AAAGGTGGAG CTGACAAACA ACAAATGGAT 
GCTGAGTTGC GGAAGGAGAT GATGGCCATC TGGCCCAATC TGTCCCAGAA GACGCTGGAC 
CTGCTGGTCA CCCCCCACAA GTCCACGGAC CTGACAGTGG GGAAGATCTA TGCGGCCATG 
ATGATCATGG AGTATTACCG GCAGAGCAAG GCCAAGAAGC TTCAGGCCAT GCGCGAGGAA 
CAGAACCGGA CACCTCTCAT GTTCCAGCGC ATGGAGCCCC CATCGCCAAC ACAGGAGGGG 
GGGCCTGGCC AGAACGCCCT CCCCTCCACC CAGCTGGACC CAGGAGGAGG CTTGATGGCT 
CACGAAAGTG GCATGAAAGA GAGCCCATCC TGGGTGACCC AGCGGGCCCA GGAGATGTTC 
CAGAAGACGG GCACATGGAG CCCAGAGCGG GGCCCCCCCA CTGATATGCC TAATAGCCAA 
CCCAACTCTC AGTCTGTGGA GATGCGAGAG ATGGGCAGAG ACGGCTACTC TGACAGTGAG 
CACTACTTCC CCGTTGAAGG CCAGGCCCGG GCCGCCTCCA TGCCCCGCCT GCCTGCCGAG 
AACCAGAGGA GAAGGGGCCG GCCACGTGGG AATAACCTCA GTACCATCTC AGACACCAGC 
CCCATGAAGC GCTCGGCCTC CGTGCTGGGC CCCAAGGCCC GGCGCCTGGA CGACTACTCC 
CTGGAGAGGG TGCCGCCCGA GGAGAACCAG CGGCACCACC AGAGGCGCAG AGACCGTGGC 
CACCGCACCT CCGAGCGCTC CTTGGGCCGC TACACGGACG TGGACACAGG CTTGGGGACA 
GACTTGAGCA TGACCACCCA GTCTGGGGAC CTGCCATCAA AGGAAAGAGA CCAGGAGCGG 
GGCCGGCCCA AGGACCGGAA GCATCGGCAA CACCACCACC ACCACCACCA CCACCATCCC 
CCGCCCCCTG ACAAGGAGCG CTACACCCAG GAGCGGCCTG AGCACGGCCG GCCCCGGGCC 
CGTGACCAGC GCTGGTCCCG CTCACCCAGT GAGGGCCGGG AGCACATGGC CCACCGTCAG 
GGCAGTAGTT CCGTAAGTGG AAGCCCAGCC CCCTCAACAT CTGGTACCAG CACTCCGCGG 
CGGGGACGCC GCCAGCTCCC CCAGACCCCC TCCACTCCCC GGCCGCACGT GTCCTATTCC 
CCCGTGATCC GTAAGGCCGG CGGCTCGGGT CCCCAGCAGC AGCAGCAGCC CGCGGCGCGG 
CCGGGCCGAA CGGCCCCCAG CGGCCCGCGG AGGTACCCGG GCCCCGTGGC CGAGCCCCTG 
GCCGGGGAGC GGCCTCCCAC GGGAGGCCAC GGAAGCAGCC GCTCGCCCGC GATGGAGCGG 
CGGATCCCGG GCCCGGCCCG GAGCGAGTCC CCCAGGGCGC CGTGTCGCCA CGGCGGGGCG 
CGATGGCCGG CGTCCGGCGC GCACGCGGCC GAGGGCCCCC CGGGCCCCCG GCACCACCAC 
GGTTACTACC GGGGCTCCGA CTACGACGAG CCAGACGGCC CGGGCGGCGG CGGCGGGGAG 
GAGGAGGAGC CCCGGGCCGC CGCCTACGAC GCGCCGCCCC CCGCGCGCCA CGGGTGCTCG 
CCCAGGACTC CCCGGGCCGC GGGCCCGGCC TGCGCCTCGC CTTCCAGGCA CGGCCGGCGA 
CTCCCGAACG GCTACTACCC GGCCCACGGA CTGGCCAGGC CCCGCGGGCC GGGCTCCCGG 
AAAGGCCTGC ACGAAGCCTA CAGCGAGACC GACGACGATG ACTGGTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1A ( XM_024993854.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849622.1 Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X7, mRNA.

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CloneID	OBa235776
Clone ID Related Accession (Same CDS sequence)	XM_024993854.1
Accession Version	XM_024993854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA 
GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA 
ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG 
AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC 
GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA 
GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG 
TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC 
GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG 
AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT 
GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC 
GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC 
CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG 
ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG 
AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG 
AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT 
GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC 
AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT 
TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC 
TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC 
AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG 
CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG 
GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT 
GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC 
TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG 
TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC 
TGGGCCGAGT ATGACCCTGC AGCGTGCGGT CGGATTCATT ATAAGGATAT GTACAGTTTA 
TTGCGCGTAA TATCTCCCCC TCTCGGCTTA GGCAAGAAAT GTCCTCATAG GGTTGCTTGC 
AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT 
ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC 
AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC 
CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG 
ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG 
GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG 
CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA 
GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG 
GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT 
ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC 
TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC 
CGCCTGCCTG CCGAGAACCA GACCATCTCA GACACCAGCC CCATGAAGCG CTCGGCCTCC 
GTGCTGGGCC CCAAGGCCCG GCGCCTGGAC GACTACTCCC TGGAGAGGGT GCCGCCCGAG 
GAGAACCAGC GGCACCACCA GAGGCGCAGA GACCGTGGCC ACCGCACCTC CGAGCGCTCC 
TTGGGCCGCT ACACGGACGT GGACACAGGC TTGGGGACAG ACTTGAGCAT GACCACCCAG 
TCTGGGGACC TGCCATCAAA GGAAAGAGAC CAGGAGCGGG GCCGGCCCAA GGACCGGAAG 
CATCGGCAAC ACCACCACCA CCACCACCAC CACCATCCCC CGCCCCCTGA CAAGGAGCGC 
TACACCCAGG AGCGGCCTGA GCACGGCCGG CCCCGGGCCC GTGACCAGCG CTGGTCCCGC 
TCACCCAGTG AGGGCCGGGA GCACATGGCC CACCGTCAGG GCAGTAGTTC CGTAAGTGGA 
AGCCCAGCCC CCTCAACATC TGGTACCAGC ACTCCGCGGC GGGGACGCCG CCAGCTCCCC 
CAGACCCCCT CCACTCCCCG GCCGCACGTG TCCTATTCCC CCGTGATCCG TAAGGCCGGC 
GGCTCGGGTC CCCAGCAGCA GCAGCAGCCC GCGGCGCGGC CGGGCCGAAC GGCCCCCAGC 
GGCCCGCGGA GGTACCCGGG CCCCGTGGCC GAGCCCCTGG CCGGGGAGCG GCCTCCCACG 
GGAGGCCACG GAAGCAGCCG CTCGCCCGCG ATGGAGCGGC GGATCCCGGG CCCGGCCCGG 
AGCGAGTCCC CCAGGGCGCC GTGTCGCCAC GGCGGGGCGC GATGGCCGGC GTCCGGCGCG 
CACGCGGCCG AGGGCCCCCC GGGCCCCCGG CACCACCACG GTTACTACCG GGGCTCCGAC 
TACGACGAGC CAGACGGCCC GGGCGGCGGC GGCGGGGAGG AGGAGGAGCC CCGGGCCGCC 
GCCTACGACG CGCCGCCCCC CGCGCGCCAC GGGTGCTCGC CCAGGACTCC CCGGGCCGCG 
GGCCCGGCCT GCGCCTCGCC TTCCAGGCAC GGCCGGCGAC TCCCGAACGG CTACTACCCG 
GCCCACGGAC TGGCCAGGCC CCGCGGGCCG GGCTCCCGGA AAGGCCTGCA CGAAGCCTAC 
AGCGAGACCG ACGACGATGA CTGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849622.1
CDS	230..7699
Translation	MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRHPFDGALRRATIKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSSSVGLDTRRPWAGSQEAELSREGPYGRESDHHAREGGLEQPGFWEGEAERGKAGDPHRRHAHRQAGSRESRSGSPRTGADGEPRRHRAHRRPGDEAPDDKADRRSRHREGSRPARGGEGESEGPDGGGERRRRHRHGPPPAYDADMRREDKERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESASPHDSLSHAGLPQSPAKMGNSMDPGPTPAPTTTATNPQNAVSRRTANNPGNPSNPGPPKTPENSLIVTNPSSTQANSAKTARKPDHTTVDIPPACPPPLNHTIIQVNKNANPDPLPKKEEEKKEEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTHECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPGQNALPSTQLDPGGGLMAHESGMKESPSWVTQRAQEMFQKTGTWSPERGPPTDMPNSQPNSQSVEMREMGRDGYSDSEHYFPVEGQARAASMPRLPAENQTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRPKDRKHRQHHHHHHHHHPPPPDKERYTQERPEHGRPRARDQRWSRSPSEGREHMAHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPSTPRPHVSYSPVIRKAGGSGPQQQQQPAARPGRTAPSGPRRYPGPVAEPLAGERPPTGGHGSSRSPAMERRIPGPARSESPRAPCRHGGARWPASGAHAAEGPPGPRHHHGYYRGSDYDEPDGPGGGGGEEEEPRAAAYDAPPPARHGCSPRTPRAAGPACASPSRHGRRLPNGYYPAHGLARPRGPGSRKGLHEAYSETDDDDWC

Target ORF information:

RefSeq Version	XM_024993854.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993854.1

ORF Insert Sequence:

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA 
GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA 
ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG 
AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC 
GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA 
GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG 
TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC 
GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG 
AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT 
GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC 
GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC 
CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG 
ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG 
AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG 
AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT 
GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC 
AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT 
TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC 
TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC 
AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG 
CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG 
GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT 
GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC 
TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG 
TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC 
TGGGCCGAGT ATGACCCTGC AGCGTGCGGT CGGATTCATT ATAAGGATAT GTACAGTTTA 
TTGCGCGTAA TATCTCCCCC TCTCGGCTTA GGCAAGAAAT GTCCTCATAG GGTTGCTTGC 
AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT 
ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC 
AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC 
CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG 
ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG 
GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG 
CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA 
GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG 
GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT 
ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC 
TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC 
CGCCTGCCTG CCGAGAACCA GACCATCTCA GACACCAGCC CCATGAAGCG CTCGGCCTCC 
GTGCTGGGCC CCAAGGCCCG GCGCCTGGAC GACTACTCCC TGGAGAGGGT GCCGCCCGAG 
GAGAACCAGC GGCACCACCA GAGGCGCAGA GACCGTGGCC ACCGCACCTC CGAGCGCTCC 
TTGGGCCGCT ACACGGACGT GGACACAGGC TTGGGGACAG ACTTGAGCAT GACCACCCAG 
TCTGGGGACC TGCCATCAAA GGAAAGAGAC CAGGAGCGGG GCCGGCCCAA GGACCGGAAG 
CATCGGCAAC ACCACCACCA CCACCACCAC CACCATCCCC CGCCCCCTGA CAAGGAGCGC 
TACACCCAGG AGCGGCCTGA GCACGGCCGG CCCCGGGCCC GTGACCAGCG CTGGTCCCGC 
TCACCCAGTG AGGGCCGGGA GCACATGGCC CACCGTCAGG GCAGTAGTTC CGTAAGTGGA 
AGCCCAGCCC CCTCAACATC TGGTACCAGC ACTCCGCGGC GGGGACGCCG CCAGCTCCCC 
CAGACCCCCT CCACTCCCCG GCCGCACGTG TCCTATTCCC CCGTGATCCG TAAGGCCGGC 
GGCTCGGGTC CCCAGCAGCA GCAGCAGCCC GCGGCGCGGC CGGGCCGAAC GGCCCCCAGC 
GGCCCGCGGA GGTACCCGGG CCCCGTGGCC GAGCCCCTGG CCGGGGAGCG GCCTCCCACG 
GGAGGCCACG GAAGCAGCCG CTCGCCCGCG ATGGAGCGGC GGATCCCGGG CCCGGCCCGG 
AGCGAGTCCC CCAGGGCGCC GTGTCGCCAC GGCGGGGCGC GATGGCCGGC GTCCGGCGCG 
CACGCGGCCG AGGGCCCCCC GGGCCCCCGG CACCACCACG GTTACTACCG GGGCTCCGAC 
TACGACGAGC CAGACGGCCC GGGCGGCGGC GGCGGGGAGG AGGAGGAGCC CCGGGCCGCC 
GCCTACGACG CGCCGCCCCC CGCGCGCCAC GGGTGCTCGC CCAGGACTCC CCGGGCCGCG 
GGCCCGGCCT GCGCCTCGCC TTCCAGGCAC GGCCGGCGAC TCCCGAACGG CTACTACCCG 
GCCCACGGAC TGGCCAGGCC CCGCGGGCCG GGCTCCCGGA AAGGCCTGCA CGAAGCCTAC 
AGCGAGACCG ACGACGATGA CTGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1A ( XM_024993855.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849623.1 Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X8, mRNA.

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CloneID	OBa235777
Clone ID Related Accession (Same CDS sequence)	XM_024993855.1
Accession Version	XM_024993855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA 
GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA 
ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG 
AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC 
GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA 
GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG 
TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC 
GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG 
AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT 
GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC 
GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC 
CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG 
ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG 
AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG 
AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT 
GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC 
AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT 
TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC 
TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC 
AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG 
CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG 
GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT 
GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC 
TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG 
TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC 
TGGGCCGAGT ATGACCCTGC AGCGTGGGGC CGCATGCCTT ACCCGGACAT GTATCAGATG 
CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC 
AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT 
ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC 
AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC 
CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG 
ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG 
GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG 
CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA 
GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG 
GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT 
ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC 
TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC 
CGCCTGCCTG CCGAGAACCA GACCATCTCA GACACCAGCC CCATGAAGCG CTCGGCCTCC 
GTGCTGGGCC CCAAGGCCCG GCGCCTGGAC GACTACTCCC TGGAGAGGGT GCCGCCCGAG 
GAGAACCAGC GGCACCACCA GAGGCGCAGA GACCGTGGCC ACCGCACCTC CGAGCGCTCC 
TTGGGCCGCT ACACGGACGT GGACACAGGC TTGGGGACAG ACTTGAGCAT GACCACCCAG 
TCTGGGGACC TGCCATCAAA GGAAAGAGAC CAGGAGCGGG GCCGGCCCAA GGACCGGAAG 
CATCGGCAAC ACCACCACCA CCACCACCAC CACCATCCCC CGCCCCCTGA CAAGGAGCGC 
TACACCCAGG AGCGGCCTGA GCACGGCCGG CCCCGGGCCC GTGACCAGCG CTGGTCCCGC 
TCACCCAGTG AGGGCCGGGA GCACATGGCC CACCGTCAGG GCAGTAGTTC CGTAAGTGGA 
AGCCCAGCCC CCTCAACATC TGGTACCAGC ACTCCGCGGC GGGGACGCCG CCAGCTCCCC 
CAGACCCCCT CCACTCCCCG GCCGCACGTG TCCTATTCCC CCGTGATCCG TAAGGCCGGC 
GGCTCGGGTC CCCAGCAGCA GCAGCAGCCC GCGGCGCGGC CGGGCCGAAC GGCCCCCAGC 
GGCCCGCGGA GGTACCCGGG CCCCGTGGCC GAGCCCCTGG CCGGGGAGCG GCCTCCCACG 
GGAGGCCACG GAAGCAGCCG CTCGCCCGCG ATGGAGCGGC GGATCCCGGG CCCGGCCCGG 
AGCGAGTCCC CCAGGGCGCC GTGTCGCCAC GGCGGGGCGC GATGGCCGGC GTCCGGCGCG 
CACGCGGCCG AGGGCCCCCC GGGCCCCCGG CACCACCACG GTTACTACCG GGGCTCCGAC 
TACGACGAGC CAGACGGCCC GGGCGGCGGC GGCGGGGAGG AGGAGGAGCC CCGGGCCGCC 
GCCTACGACG CGCCGCCCCC CGCGCGCCAC GGGTGCTCGC CCAGGACTCC CCGGGCCGCG 
GGCCCGGCCT GCGCCTCGCC TTCCAGGCAC GGCCGGCGAC TCCCGAACGG CTACTACCCG 
GCCCACGGAC TGGCCAGGCC CCGCGGGCCG GGCTCCCGGA AAGGCCTGCA CGAAGCCTAC 
AGCGAGACCG ACGACGATGA CTGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849623.1
CDS	230..7699
Translation	MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRHPFDGALRRATIKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSSSVGLDTRRPWAGSQEAELSREGPYGRESDHHAREGGLEQPGFWEGEAERGKAGDPHRRHAHRQAGSRESRSGSPRTGADGEPRRHRAHRRPGDEAPDDKADRRSRHREGSRPARGGEGESEGPDGGGERRRRHRHGPPPAYDADMRREDKERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESASPHDSLSHAGLPQSPAKMGNSMDPGPTPAPTTTATNPQNAVSRRTANNPGNPSNPGPPKTPENSLIVTNPSSTQANSAKTARKPDHTTVDIPPACPPPLNHTIIQVNKNANPDPLPKKEEEKKEEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTHECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMPYPDMYQMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPGQNALPSTQLDPGGGLMAHESGMKESPSWVTQRAQEMFQKTGTWSPERGPPTDMPNSQPNSQSVEMREMGRDGYSDSEHYFPVEGQARAASMPRLPAENQTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRPKDRKHRQHHHHHHHHHPPPPDKERYTQERPEHGRPRARDQRWSRSPSEGREHMAHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPSTPRPHVSYSPVIRKAGGSGPQQQQQPAARPGRTAPSGPRRYPGPVAEPLAGERPPTGGHGSSRSPAMERRIPGPARSESPRAPCRHGGARWPASGAHAAEGPPGPRHHHGYYRGSDYDEPDGPGGGGGEEEEPRAAAYDAPPPARHGCSPRTPRAAGPACASPSRHGRRLPNGYYPAHGLARPRGPGSRKGLHEAYSETDDDDWC

Target ORF information:

RefSeq Version	XM_024993855.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993855.1

ORF Insert Sequence:

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA 
GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA 
ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG 
AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC 
GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA 
GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG 
TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC 
GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG 
AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT 
GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC 
GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC 
CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG 
ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG 
AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG 
AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT 
GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC 
AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT 
TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC 
TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC 
AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG 
CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG 
GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT 
GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC 
TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG 
TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC 
TGGGCCGAGT ATGACCCTGC AGCGTGGGGC CGCATGCCTT ACCCGGACAT GTATCAGATG 
CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC 
AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT 
ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC 
AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC 
CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG 
ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG 
GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG 
CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA 
GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG 
GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT 
ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC 
TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC 
CGCCTGCCTG CCGAGAACCA GACCATCTCA GACACCAGCC CCATGAAGCG CTCGGCCTCC 
GTGCTGGGCC CCAAGGCCCG GCGCCTGGAC GACTACTCCC TGGAGAGGGT GCCGCCCGAG 
GAGAACCAGC GGCACCACCA GAGGCGCAGA GACCGTGGCC ACCGCACCTC CGAGCGCTCC 
TTGGGCCGCT ACACGGACGT GGACACAGGC TTGGGGACAG ACTTGAGCAT GACCACCCAG 
TCTGGGGACC TGCCATCAAA GGAAAGAGAC CAGGAGCGGG GCCGGCCCAA GGACCGGAAG 
CATCGGCAAC ACCACCACCA CCACCACCAC CACCATCCCC CGCCCCCTGA CAAGGAGCGC 
TACACCCAGG AGCGGCCTGA GCACGGCCGG CCCCGGGCCC GTGACCAGCG CTGGTCCCGC 
TCACCCAGTG AGGGCCGGGA GCACATGGCC CACCGTCAGG GCAGTAGTTC CGTAAGTGGA 
AGCCCAGCCC CCTCAACATC TGGTACCAGC ACTCCGCGGC GGGGACGCCG CCAGCTCCCC 
CAGACCCCCT CCACTCCCCG GCCGCACGTG TCCTATTCCC CCGTGATCCG TAAGGCCGGC 
GGCTCGGGTC CCCAGCAGCA GCAGCAGCCC GCGGCGCGGC CGGGCCGAAC GGCCCCCAGC 
GGCCCGCGGA GGTACCCGGG CCCCGTGGCC GAGCCCCTGG CCGGGGAGCG GCCTCCCACG 
GGAGGCCACG GAAGCAGCCG CTCGCCCGCG ATGGAGCGGC GGATCCCGGG CCCGGCCCGG 
AGCGAGTCCC CCAGGGCGCC GTGTCGCCAC GGCGGGGCGC GATGGCCGGC GTCCGGCGCG 
CACGCGGCCG AGGGCCCCCC GGGCCCCCGG CACCACCACG GTTACTACCG GGGCTCCGAC 
TACGACGAGC CAGACGGCCC GGGCGGCGGC GGCGGGGAGG AGGAGGAGCC CCGGGCCGCC 
GCCTACGACG CGCCGCCCCC CGCGCGCCAC GGGTGCTCGC CCAGGACTCC CCGGGCCGCG 
GGCCCGGCCT GCGCCTCGCC TTCCAGGCAC GGCCGGCGAC TCCCGAACGG CTACTACCCG 
GCCCACGGAC TGGCCAGGCC CCGCGGGCCG GGCTCCCGGA AAGGCCTGCA CGAAGCCTAC 
AGCGAGACCG ACGACGATGA CTGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1A ( XM_024993856.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849624.1 Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X9, mRNA.

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CloneID	OBa235778
Clone ID Related Accession (Same CDS sequence)	XM_024993856.1
Accession Version	XM_024993856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA 
GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA 
ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG 
AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC 
GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA 
GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG 
TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC 
GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG 
AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT 
GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC 
GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC 
CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG 
ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG 
AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG 
AAAGTCATGG CTTTTGGGAT TCTGAATAAC TTCATCAACT TGAGCTTCCT CCGCCTCTTC 
CGAGCTGCCC GGCTCATCAA ACTCCTCCGT CAGGGTTACA CCATCCGAAT TCTTCTCTGG 
ACCTTTGTGC AGTCCTTCAA GGCCCTGCCC TATGTCTGTC TACTGATTGC CATGCTTTTT 
TTCATCTACG CCATCATTGG GATGCAGGTG TTTGGCAACA TTGGCATCGA TGTGGAGGAC 
GAGGACAGTG ATGAAGATGA ATTCCAAATC ACTGAGCACA ATAACTTCCG CACCTTCTTC 
CAGGCCCTCA TGCTTCTCTT CCGGAGTGCC ACTGGGGAGG CTTGGCACAA CATCATGCTT 
TCCTGCCTCA GCGGGAAACC CTGTGATAAG AACTCTGGCA TCCTGACTCA CGAATGCGGC 
AATGAATTTG CTTATTTTTA CTTCGTTTCC TTCATCTTCC TCTGCTCGTT TCTGATGCTG 
AATCTTTTTG TTGCCGTCAT CATGGACAAC TTTGAGTACC TCACCCGAGA TTCCTCCATC 
CTGGGTCCCC ACCACCTGGA TGAGTACGTG CGTGTCTGGG CCGAGTATGA CCCTGCAGCG 
TGCGGTCGGA TTCATTATAA GGATATGTAC AGTTTATTGC GCGTAATATC TCCCCCTCTC 
GGCTTAGGCA AGAAATGTCC TCATAGGGTT GCTTGCAAGA GGCTCCTGCG GATGGATCTG 
CCCGTCGCTG ATGACAACAC CGTCCACTTC AACTCTACCC TCATGGCTCT GATCCGCACG 
GCCCTGGACA TCAAAATTGC CAAAGGTGGA GCTGACAAAC AACAAATGGA TGCTGAGTTG 
CGGAAGGAGA TGATGGCCAT CTGGCCCAAT CTGTCCCAGA AGACGCTGGA CCTGCTGGTC 
ACCCCCCACA AGTCCACGGA CCTGACAGTG GGGAAGATCT ATGCGGCCAT GATGATCATG 
GAGTATTACC GGCAGAGCAA GGCCAAGAAG CTTCAGGCCA TGCGCGAGGA ACAGAACCGG 
ACACCTCTCA TGTTCCAGCG CATGGAGCCC CCATCGCCAA CACAGGAGGG GGGGCCTGGC 
CAGAACGCCC TCCCCTCCAC CCAGCTGGAC CCAGGAGGAG GCTTGATGGC TCACGAAAGT 
GGCATGAAAG AGAGCCCATC CTGGGTGACC CAGCGGGCCC AGGAGATGTT CCAGAAGACG 
GGCACATGGA GCCCAGAGCG GGGCCCCCCC ACTGATATGC CTAATAGCCA ACCCAACTCT 
CAGTCTGTGG AGATGCGAGA GATGGGCAGA GACGGCTACT CTGACAGTGA GCACTACTTC 
CCCGTTGAAG GCCAGGCCCG GGCCGCCTCC ATGCCCCGCC TGCCTGCCGA GAACCAGAGG 
AGAAGGGGCC GGCCACGTGG GAATAACCTC AGTACCATCT CAGACACCAG CCCCATGAAG 
CGCTCGGCCT CCGTGCTGGG CCCCAAGGCC CGGCGCCTGG ACGACTACTC CCTGGAGAGG 
GTGCCGCCCG AGGAGAACCA GCGGCACCAC CAGAGGCGCA GAGACCGTGG CCACCGCACC 
TCCGAGCGCT CCTTGGGCCG CTACACGGAC GTGGACACAG GCTTGGGGAC AGACTTGAGC 
ATGACCACCC AGTCTGGGGA CCTGCCATCA AAGGAAAGAG ACCAGGAGCG GGGCCGGCCC 
AAGGACCGGA AGCATCGGCA ACACCACCAC CACCACCACC ACCACCATCC CCCGCCCCCT 
GACAAGGAGC GCTACACCCA GGAGCGGCCT GAGCACGGCC GGCCCCGGGC CCGTGACCAG 
CGCTGGTCCC GCTCACCCAG TGAGGGCCGG GAGCACATGG CCCACCGTCA GGGCAGTAGT 
TCCGTAAGTG GAAGCCCAGC CCCCTCAACA TCTGGTACCA GCACTCCGCG GCGGGGACGC 
CGCCAGCTCC CCCAGACCCC CTCCACTCCC CGGCCGCACG TGTCCTATTC CCCCGTGATC 
CGTAAGGCCG GCGGCTCGGG TCCCCAGCAG CAGCAGCAGC CCGCGGCGCG GCCGGGCCGA 
ACGGCCCCCA GCGGCCCGCG GAGGTACCCG GGCCCCGTGG CCGAGCCCCT GGCCGGGGAG 
CGGCCTCCCA CGGGAGGCCA CGGAAGCAGC CGCTCGCCCG CGATGGAGCG GCGGATCCCG 
GGCCCGGCCC GGAGCGAGTC CCCCAGGGCG CCGTGTCGCC ACGGCGGGGC GCGATGGCCG 
GCGTCCGGCG CGCACGCGGC CGAGGGCCCC CCGGGCCCCC GGCACCACCA CGGTTACTAC 
CGGGGCTCCG ACTACGACGA GCCAGACGGC CCGGGCGGCG GCGGCGGGGA GGAGGAGGAG 
CCCCGGGCCG CCGCCTACGA CGCGCCGCCC CCCGCGCGCC ACGGGTGCTC GCCCAGGACT 
CCCCGGGCCG CGGGCCCGGC CTGCGCCTCG CCTTCCAGGC ACGGCCGGCG ACTCCCGAAC 
GGCTACTACC CGGCCCACGG ACTGGCCAGG CCCCGCGGGC CGGGCTCCCG GAAAGGCCTG 
CACGAAGCCT ACAGCGAGAC CGACGACGAT GACTGGTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849624.1
CDS	231..7652
Translation	MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRHPFDGALRRATIKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSSSVGLDTRRPWAGSQEAELSREGPYGRESDHHAREGGLEQPGFWEGEAERGKAGDPHRRHAHRQAGSRESRSGSPRTGADGEPRRHRAHRRPGDEAPDDKADRRSRHREGSRPARGGEGESEGPDGGGERRRRHRHGPPPAYDADMRREDKERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESASPHDSLSHAGLPQSPAKMGNSMDPGPTPAPTTTATNPQNAVSRRTANNPGNPSNPGPPKTPENSLIVTNPSSTQANSAKTARKPDHTTVDIPPACPPPLNHTIIQVNKNANPDPLPKKEEEKKEEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTHECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPGQNALPSTQLDPGGGLMAHESGMKESPSWVTQRAQEMFQKTGTWSPERGPPTDMPNSQPNSQSVEMREMGRDGYSDSEHYFPVEGQARAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRPKDRKHRQHHHHHHHHHPPPPDKERYTQERPEHGRPRARDQRWSRSPSEGREHMAHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPSTPRPHVSYSPVIRKAGGSGPQQQQQPAARPGRTAPSGPRRYPGPVAEPLAGERPPTGGHGSSRSPAMERRIPGPARSESPRAPCRHGGARWPASGAHAAEGPPGPRHHHGYYRGSDYDEPDGPGGGGGEEEEPRAAAYDAPPPARHGCSPRTPRAAGPACASPSRHGRRLPNGYYPAHGLARPRGPGSRKGLHEAYSETDDDDWC

Target ORF information:

RefSeq Version	XM_024993856.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993856.1

ORF Insert Sequence:

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA 
GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA 
ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG 
AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC 
GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA 
GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG 
TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC 
GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG 
AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT 
GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC 
GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC 
CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG 
ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG 
AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG 
AAAGTCATGG CTTTTGGGAT TCTGAATAAC TTCATCAACT TGAGCTTCCT CCGCCTCTTC 
CGAGCTGCCC GGCTCATCAA ACTCCTCCGT CAGGGTTACA CCATCCGAAT TCTTCTCTGG 
ACCTTTGTGC AGTCCTTCAA GGCCCTGCCC TATGTCTGTC TACTGATTGC CATGCTTTTT 
TTCATCTACG CCATCATTGG GATGCAGGTG TTTGGCAACA TTGGCATCGA TGTGGAGGAC 
GAGGACAGTG ATGAAGATGA ATTCCAAATC ACTGAGCACA ATAACTTCCG CACCTTCTTC 
CAGGCCCTCA TGCTTCTCTT CCGGAGTGCC ACTGGGGAGG CTTGGCACAA CATCATGCTT 
TCCTGCCTCA GCGGGAAACC CTGTGATAAG AACTCTGGCA TCCTGACTCA CGAATGCGGC 
AATGAATTTG CTTATTTTTA CTTCGTTTCC TTCATCTTCC TCTGCTCGTT TCTGATGCTG 
AATCTTTTTG TTGCCGTCAT CATGGACAAC TTTGAGTACC TCACCCGAGA TTCCTCCATC 
CTGGGTCCCC ACCACCTGGA TGAGTACGTG CGTGTCTGGG CCGAGTATGA CCCTGCAGCG 
TGCGGTCGGA TTCATTATAA GGATATGTAC AGTTTATTGC GCGTAATATC TCCCCCTCTC 
GGCTTAGGCA AGAAATGTCC TCATAGGGTT GCTTGCAAGA GGCTCCTGCG GATGGATCTG 
CCCGTCGCTG ATGACAACAC CGTCCACTTC AACTCTACCC TCATGGCTCT GATCCGCACG 
GCCCTGGACA TCAAAATTGC CAAAGGTGGA GCTGACAAAC AACAAATGGA TGCTGAGTTG 
CGGAAGGAGA TGATGGCCAT CTGGCCCAAT CTGTCCCAGA AGACGCTGGA CCTGCTGGTC 
ACCCCCCACA AGTCCACGGA CCTGACAGTG GGGAAGATCT ATGCGGCCAT GATGATCATG 
GAGTATTACC GGCAGAGCAA GGCCAAGAAG CTTCAGGCCA TGCGCGAGGA ACAGAACCGG 
ACACCTCTCA TGTTCCAGCG CATGGAGCCC CCATCGCCAA CACAGGAGGG GGGGCCTGGC 
CAGAACGCCC TCCCCTCCAC CCAGCTGGAC CCAGGAGGAG GCTTGATGGC TCACGAAAGT 
GGCATGAAAG AGAGCCCATC CTGGGTGACC CAGCGGGCCC AGGAGATGTT CCAGAAGACG 
GGCACATGGA GCCCAGAGCG GGGCCCCCCC ACTGATATGC CTAATAGCCA ACCCAACTCT 
CAGTCTGTGG AGATGCGAGA GATGGGCAGA GACGGCTACT CTGACAGTGA GCACTACTTC 
CCCGTTGAAG GCCAGGCCCG GGCCGCCTCC ATGCCCCGCC TGCCTGCCGA GAACCAGAGG 
AGAAGGGGCC GGCCACGTGG GAATAACCTC AGTACCATCT CAGACACCAG CCCCATGAAG 
CGCTCGGCCT CCGTGCTGGG CCCCAAGGCC CGGCGCCTGG ACGACTACTC CCTGGAGAGG 
GTGCCGCCCG AGGAGAACCA GCGGCACCAC CAGAGGCGCA GAGACCGTGG CCACCGCACC 
TCCGAGCGCT CCTTGGGCCG CTACACGGAC GTGGACACAG GCTTGGGGAC AGACTTGAGC 
ATGACCACCC AGTCTGGGGA CCTGCCATCA AAGGAAAGAG ACCAGGAGCG GGGCCGGCCC 
AAGGACCGGA AGCATCGGCA ACACCACCAC CACCACCACC ACCACCATCC CCCGCCCCCT 
GACAAGGAGC GCTACACCCA GGAGCGGCCT GAGCACGGCC GGCCCCGGGC CCGTGACCAG 
CGCTGGTCCC GCTCACCCAG TGAGGGCCGG GAGCACATGG CCCACCGTCA GGGCAGTAGT 
TCCGTAAGTG GAAGCCCAGC CCCCTCAACA TCTGGTACCA GCACTCCGCG GCGGGGACGC 
CGCCAGCTCC CCCAGACCCC CTCCACTCCC CGGCCGCACG TGTCCTATTC CCCCGTGATC 
CGTAAGGCCG GCGGCTCGGG TCCCCAGCAG CAGCAGCAGC CCGCGGCGCG GCCGGGCCGA 
ACGGCCCCCA GCGGCCCGCG GAGGTACCCG GGCCCCGTGG CCGAGCCCCT GGCCGGGGAG 
CGGCCTCCCA CGGGAGGCCA CGGAAGCAGC CGCTCGCCCG CGATGGAGCG GCGGATCCCG 
GGCCCGGCCC GGAGCGAGTC CCCCAGGGCG CCGTGTCGCC ACGGCGGGGC GCGATGGCCG 
GCGTCCGGCG CGCACGCGGC CGAGGGCCCC CCGGGCCCCC GGCACCACCA CGGTTACTAC 
CGGGGCTCCG ACTACGACGA GCCAGACGGC CCGGGCGGCG GCGGCGGGGA GGAGGAGGAG 
CCCCGGGCCG CCGCCTACGA CGCGCCGCCC CCCGCGCGCC ACGGGTGCTC GCCCAGGACT 
CCCCGGGCCG CGGGCCCGGC CTGCGCCTCG CCTTCCAGGC ACGGCCGGCG ACTCCCGAAC 
GGCTACTACC CGGCCCACGG ACTGGCCAGG CCCCGCGGGC CGGGCTCCCG GAAAGGCCTG 
CACGAAGCCT ACAGCGAGAC CGACGACGAT GACTGGTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1A ( XM_024993858.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849626.1 Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X10, mRNA.

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CloneID	OBa235779
Clone ID Related Accession (Same CDS sequence)	XM_024993858.1
Accession Version	XM_024993858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGAC TATGCAGAAT TCATTTTCTT AGGACTCTTT 
ATGTCCGAAA TGTTTATAAA AATGTACGGG CTTGGGACGC GGCCTTACTT CCACTCTTCC 
TTCAACTGTT TTGATTGTGG GGTCATCATC GGGAGTATCT TTGAGGTCAT CTGGGCTGTC 
ATAAAACCCG GCACATCCTT TGGAATCAGT GTGTTACGAG CCCTCAGGTT ATTGCGTATT 
TTCAAAGTCA CAAAGTACTG GGCATCTCTC AGGAACCTGG TCGTCTCTCT TCTCAACTCC 
ATGAAATCCA TCATCAGCCT GTTGTTCCTC CTTTTCCTAT TCATCGTCGT CTTTGCCCTT 
CTGGGAATGC AACTCTTTGG CGGCCAGTTT AATTTCGATG AAGGGACTCC TCCTACCAAC 
TTCGACACTT TTCCAGCAGC AATAATGACA GTTTTTCAGA TCCTGACGGG CGAAGACTGG 
AACGAGGTCA TGTATGACGG CATCAAGTCT CAGGGGGGCG TGCAAGGCGG CATGGTGTTC 
TCCATCTACT TCATCGTGCT GACGCTCTTC GGGAACTACA CCCTCCTGAA CGTGTTCTTG 
GCCATCGCGG TGGATAATCT GGCTAACGCC CAGGAGCTCA CCAAGGATGA ACAAGAAGAA 
GAAGAGGCTG CCAACCAGAA ACTCGCCCTC CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT 
CCTCTGTCTG CAGCCAACAT GTCCATAGCT GTGAAGGAGC AACAGAAGAA CCAGAAGCCC 
GCCAAGTCGG TGTGGGAGCA GCGGACAAGC GAGATGAGGA AGCAGAATCT TCTGGCCAGT 
CGGGAGGCAC TCTACAACGA GATGGACCCA GACGAGCGCT GGAAGGCCTC CTACGCTCGC 
CACCTGCGGC CCGACATGAA GACCCACCTG GACCGGCCGC TGGTGGTCGA CCCCCAGGAG 
AACCGTAACA ACAACACCAA CAAGAGCCGG GCGGCCGAGC CCACGGTGGA CCAGCGCCTC 
GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG AAACAGGCCC GCTACCACGA CCGGGCCCGG 
GACCCCAGCA GCTCCGTGGG CCTGGACACC CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC 
GAGCTGAGCC GGGAGGGGCC TTACGGTCGC GAGTCGGACC ACCACGCCCG GGAGGGCGGC 
CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG GCGGAGCGGG GCAAGGCGGG CGACCCGCAC 
CGGAGGCACG CGCACCGGCA GGCGGGCAGC CGCGAGAGCC GCAGCGGGTC CCCGCGCACC 
GGGGCCGACG GAGAGCCCCG CCGGCACCGG GCGCACCGCA GGCCGGGCGA CGAGGCCCCG 
GACGACAAGG CCGATCGGAG GTCCCGGCAC CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC 
GAGGGCGAGA GCGAGGGCCC AGACGGCGGC GGCGAGCGGA GGCGGCGGCA CCGGCATGGC 
CCTCCCCCAG CGTACGACGC GGACATGCGC AGGGAGGACA AGGAGCGCAG GCACCGCAGG 
AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC GTGTCAGGCC CCAACCTGTC GACCACACGG 
CCAATCCAGC AGGACCTGGG CCGCCAAGAC CCCCCGCTGG CCGAGGATAT CGACAACATG 
AAGAACAACA AACTGGCCAC TGCGGAGTCA GCCAGTCCCC ACGACAGCCT CAGCCACGCC 
GGCCTGCCCC AGAGCCCAGC CAAGATGGGG AACAGCATGG ACCCTGGCCC CACACCGGCC 
CCCACCACCA CGGCCACCAA CCCTCAGAAT GCCGTCAGTC GCCGGACAGC CAACAACCCG 
GGGAACCCGT CCAACCCCGG CCCCCCCAAG ACCCCTGAGA ACAGCCTTAT CGTCACCAAC 
CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG ACTGCCAGGA AACCTGACCA CACCACGGTG 
GACATCCCCC CAGCCTGCCC ACCCCCACTC AACCACACCA TCATCCAAGT GAACAAAAAC 
GCCAACCCAG ACCCACTGCC AAAAAAGGAG GAAGAGAAGA AAGAGGAGGA GGAAGACGAC 
CCCGGGGAAG ACGGCCCCAA GCCCATGCCC CCCTACAGCT CCATGTTTAT CCTCTCTACG 
ACCAACCCCC TTCGCCGCCT GTGCCATTAC ATCCTGAACC TGCGCTACTT TGAGATGTGC 
ATTCTCATGG TCATCGCCAT GAGCAGCATC GCCCTGGCGG CCGAGGATCC TGTTCAGCCC 
AACGCACCAC GAAACAATGT GCTGCGATAT TTTGACTACG TTTTTACAGG CGTCTTTACC 
TTTGAGATGG TGATCAAGAT GATCGACCTG GGGCTCGTCC TGCACCAGGG TGCCTACTTC 
CGTGACCTCT GGAACATTCT CGACTTCATA GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC 
TTCACGAGCA GTACACGTGG CAATAGCAAA GGAAAGGACA TCAACACGAT TAAATCCCTC 
CGAGTCCTCC GGGTGCTACG ACCTCTTAAA ACCATCAAGC GGTTGCCAAA GCTCAAGGCC 
GTGTTTGACT GCGTGGTGAA CTCTCTCAAG AATGTCTTCA ACATCCTCAT CGTCTACATG 
CTGTTCATGT TCATCTTCGC CGTGGTGGCC GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC 
TGCACAGATG AGTCCAAGGA GTTTGAGAAA GATTGCCGCG GCAAGTACCT CCTCTATGAG 
AAGAATGAGG TAAAGGCGAG GGATCGAGAG TGGAAGAAGT ATGAGTTCCA TTATGACAAT 
GTGCTGTGGG CGCTGCTGAC CCTCTTCACC GTGTCCACGG GAGAAGGCTG GCCACAGGTC 
CTCAAGCACT CCGTGGACGC CACCTTCGAG AACCAGGGCC CCAGCCCCGG GTACCGCATG 
GAGATGTCCA TCTTCTACGT CGTCTACTTT GTGGTGTTCC CCTTCTTCTT TGTCAACATC 
TTTGTGGCCT TGATCATCAT CACCTTCCAG GAGCAGGGGG ACAAGATGAT GGAGGAGTAC 
AGCCTGGAGA AAAATGAGAG GGCGTGCATC GACTTCGCCA TCAGCGCCAA ACCCCTGACC 
CGGCACATGC CGCAGAACAA GCAGAGTTTC CAGTACCGCA TGTGGCAGTT CGTGGTGTCC 
CCGCCTTTCG AGTACACCAT CATGGCCATG ATCGCCCTCA ACACCATCGT GCTCATGATG 
AAGTTCTACG GGGCCTCTGT TGCTTATGAG AATGCTCTGA GAGTATTCAA CATCGTCTTC 
ACCTCCCTCT TCTCACTGGA ATGTGTGCTG AAAGTCATGG CTTTTGGGAT TCTGAATTAT 
TTTCGCGATG CCTGGAACAT CTTCGACTTT GTGACTGTTC TGGGCAGCAT CACCGACATC 
CTCGTGACCG AGTTTGGGAA TAACTTCATC AACTTGAGCT TCCTCCGCCT CTTCCGAGCT 
GCCCGGCTCA TCAAACTCCT CCGTCAGGGT TACACCATCC GAATTCTTCT CTGGACCTTT 
GTGCAGTCCT TCAAGGCCCT GCCCTATGTC TGTCTACTGA TTGCCATGCT TTTTTTCATC 
TACGCCATCA TTGGGATGCA GGTGTTTGGC AACATTGGCA TCGATGTGGA GGACGAGGAC 
AGTGATGAAG ATGAATTCCA AATCACTGAG CACAATAACT TCCGCACCTT CTTCCAGGCC 
CTCATGCTTC TCTTCCGGAG TGCCACTGGG GAGGCTTGGC ACAACATCAT GCTTTCCTGC 
CTCAGCGGGA AACCCTGTGA TAAGAACTCT GGCATCCTGA CTCACGAATG CGGCAATGAA 
TTTGCTTATT TTTACTTCGT TTCCTTCATC TTCCTCTGCT CGTTTCTGAT GCTGAATCTT 
TTTGTTGCCG TCATCATGGA CAACTTTGAG TACCTCACCC GAGATTCCTC CATCCTGGGT 
CCCCACCACC TGGATGAGTA CGTGCGTGTC TGGGCCGAGT ATGACCCTGC AGCGTGCGGT 
CGGATTCATT ATAAGGATAT GTACAGTTTA TTGCGCGTAA TATCTCCCCC TCTCGGCTTA 
GGCAAGAAAT GTCCTCATAG GGTTGCTTGC AAGAGGCTCC TGCGGATGGA TCTGCCCGTC 
GCTGATGACA ACACCGTCCA CTTCAACTCT ACCCTCATGG CTCTGATCCG CACGGCCCTG 
GACATCAAAA TTGCCAAAGG TGGAGCTGAC AAACAACAAA TGGATGCTGA GTTGCGGAAG 
GAGATGATGG CCATCTGGCC CAATCTGTCC CAGAAGACGC TGGACCTGCT GGTCACCCCC 
CACAAGTCCA CGGACCTGAC AGTGGGGAAG ATCTATGCGG CCATGATGAT CATGGAGTAT 
TACCGGCAGA GCAAGGCCAA GAAGCTTCAG GCCATGCGCG AGGAACAGAA CCGGACACCT 
CTCATGTTCC AGCGCATGGA GCCCCCATCG CCAACACAGG AGGGGGGGCC TGGCCAGAAC 
GCCCTCCCCT CCACCCAGCT GGACCCAGGA GGAGGCTTGA TGGCTCACGA AAGTGGCATG 
AAAGAGAGCC CATCCTGGGT GACCCAGCGG GCCCAGGAGA TGTTCCAGAA GACGGGCACA 
TGGAGCCCAG AGCGGGGCCC CCCCACTGAT ATGCCTAATA GCCAACCCAA CTCTCAGTCT 
GTGGAGATGC GAGAGATGGG CAGAGACGGC TACTCTGACA GTGAGCACTA CTTCCCCGTT 
GAAGGCCAGG CCCGGGCCGC CTCCATGCCC CGCCTGCCTG CCGAGAACCA GAGGAGAAGG 
GGCCGGCCAC GTGGGAATAA CCTCAGTACC ATCTCAGACA CCAGCCCCAT GAAGCGCTCG 
GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC CTGGACGACT ACTCCCTGGA GAGGGTGCCG 
CCCGAGGAGA ACCAGCGGCA CCACCAGAGG CGCAGAGACC GTGGCCACCG CACCTCCGAG 
CGCTCCTTGG GCCGCTACAC GGACGTGGAC ACAGGCTTGG GGACAGACTT GAGCATGACC 
ACCCAGTCTG GGGACCTGCC ATCAAAGGAA AGAGACCAGG AGCGGGGCCG GCCCAAGGAC 
CGGAAGCATC GGCAACACCA CCACCACCAC CACCACCACC ATCCCCCGCC CCCTGACAAG 
GAGCGCTACA CCCAGGAGCG GCCTGAGCAC GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG 
TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC ATGGCCCACC GTCAGGGCAG TAGTTCCGTA 
AGTGGAAGCC CAGCCCCCTC AACATCTGGT ACCAGCACTC CGCGGCGGGG ACGCCGCCAG 
CTCCCCCAGA CCCCCTCCAC TCCCCGGCCG CACGTGTCCT ATTCCCCCGT GATCCGTAAG 
GCCGGCGGCT CGGGTCCCCA GCAGCAGCAG CAGCCCGCGG CGCGGCCGGG CCGAACGGCC 
CCCAGCGGCC CGCGGAGGTA CCCGGGCCCC GTGGCCGAGC CCCTGGCCGG GGAGCGGCCT 
CCCACGGGAG GCCACGGAAG CAGCCGCTCG CCCGCGATGG AGCGGCGGAT CCCGGGCCCG 
GCCCGGAGCG AGTCCCCCAG GGCGCCGTGT CGCCACGGCG GGGCGCGATG GCCGGCGTCC 
GGCGCGCACG CGGCCGAGGG CCCCCCGGGC CCCCGGCACC ACCACGGTTA CTACCGGGGC 
TCCGACTACG ACGAGCCAGA CGGCCCGGGC GGCGGCGGCG GGGAGGAGGA GGAGCCCCGG 
GCCGCCGCCT ACGACGCGCC GCCCCCCGCG CGCCACGGGT GCTCGCCCAG GACTCCCCGG 
GCCGCGGGCC CGGCCTGCGC CTCGCCTTCC AGGCACGGCC GGCGACTCCC GAACGGCTAC 
TACCCGGCCC ACGGACTGGC CAGGCCCCGC GGGCCGGGCT CCCGGAAAGG CCTGCACGAA 
GCCTACAGCG AGACCGACGA CGATGACTGG TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849626.1
CDS	230..7525
Translation	MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRHPFDGALRRATIKKSKTDLLNPEEAEDQLADIASVDYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSSSVGLDTRRPWAGSQEAELSREGPYGRESDHHAREGGLEQPGFWEGEAERGKAGDPHRRHAHRQAGSRESRSGSPRTGADGEPRRHRAHRRPGDEAPDDKADRRSRHREGSRPARGGEGESEGPDGGGERRRRHRHGPPPAYDADMRREDKERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESASPHDSLSHAGLPQSPAKMGNSMDPGPTPAPTTTATNPQNAVSRRTANNPGNPSNPGPPKTPENSLIVTNPSSTQANSAKTARKPDHTTVDIPPACPPPLNHTIIQVNKNANPDPLPKKEEEKKEEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTHECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPGQNALPSTQLDPGGGLMAHESGMKESPSWVTQRAQEMFQKTGTWSPERGPPTDMPNSQPNSQSVEMREMGRDGYSDSEHYFPVEGQARAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRPKDRKHRQHHHHHHHHHPPPPDKERYTQERPEHGRPRARDQRWSRSPSEGREHMAHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPSTPRPHVSYSPVIRKAGGSGPQQQQQPAARPGRTAPSGPRRYPGPVAEPLAGERPPTGGHGSSRSPAMERRIPGPARSESPRAPCRHGGARWPASGAHAAEGPPGPRHHHGYYRGSDYDEPDGPGGGGGEEEEPRAAAYDAPPPARHGCSPRTPRAAGPACASPSRHGRRLPNGYYPAHGLARPRGPGSRKGLHEAYSETDDDDWC

Target ORF information:

RefSeq Version	XM_024993858.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993858.1

ORF Insert Sequence:

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGAC TATGCAGAAT TCATTTTCTT AGGACTCTTT 
ATGTCCGAAA TGTTTATAAA AATGTACGGG CTTGGGACGC GGCCTTACTT CCACTCTTCC 
TTCAACTGTT TTGATTGTGG GGTCATCATC GGGAGTATCT TTGAGGTCAT CTGGGCTGTC 
ATAAAACCCG GCACATCCTT TGGAATCAGT GTGTTACGAG CCCTCAGGTT ATTGCGTATT 
TTCAAAGTCA CAAAGTACTG GGCATCTCTC AGGAACCTGG TCGTCTCTCT TCTCAACTCC 
ATGAAATCCA TCATCAGCCT GTTGTTCCTC CTTTTCCTAT TCATCGTCGT CTTTGCCCTT 
CTGGGAATGC AACTCTTTGG CGGCCAGTTT AATTTCGATG AAGGGACTCC TCCTACCAAC 
TTCGACACTT TTCCAGCAGC AATAATGACA GTTTTTCAGA TCCTGACGGG CGAAGACTGG 
AACGAGGTCA TGTATGACGG CATCAAGTCT CAGGGGGGCG TGCAAGGCGG CATGGTGTTC 
TCCATCTACT TCATCGTGCT GACGCTCTTC GGGAACTACA CCCTCCTGAA CGTGTTCTTG 
GCCATCGCGG TGGATAATCT GGCTAACGCC CAGGAGCTCA CCAAGGATGA ACAAGAAGAA 
GAAGAGGCTG CCAACCAGAA ACTCGCCCTC CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT 
CCTCTGTCTG CAGCCAACAT GTCCATAGCT GTGAAGGAGC AACAGAAGAA CCAGAAGCCC 
GCCAAGTCGG TGTGGGAGCA GCGGACAAGC GAGATGAGGA AGCAGAATCT TCTGGCCAGT 
CGGGAGGCAC TCTACAACGA GATGGACCCA GACGAGCGCT GGAAGGCCTC CTACGCTCGC 
CACCTGCGGC CCGACATGAA GACCCACCTG GACCGGCCGC TGGTGGTCGA CCCCCAGGAG 
AACCGTAACA ACAACACCAA CAAGAGCCGG GCGGCCGAGC CCACGGTGGA CCAGCGCCTC 
GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG AAACAGGCCC GCTACCACGA CCGGGCCCGG 
GACCCCAGCA GCTCCGTGGG CCTGGACACC CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC 
GAGCTGAGCC GGGAGGGGCC TTACGGTCGC GAGTCGGACC ACCACGCCCG GGAGGGCGGC 
CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG GCGGAGCGGG GCAAGGCGGG CGACCCGCAC 
CGGAGGCACG CGCACCGGCA GGCGGGCAGC CGCGAGAGCC GCAGCGGGTC CCCGCGCACC 
GGGGCCGACG GAGAGCCCCG CCGGCACCGG GCGCACCGCA GGCCGGGCGA CGAGGCCCCG 
GACGACAAGG CCGATCGGAG GTCCCGGCAC CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC 
GAGGGCGAGA GCGAGGGCCC AGACGGCGGC GGCGAGCGGA GGCGGCGGCA CCGGCATGGC 
CCTCCCCCAG CGTACGACGC GGACATGCGC AGGGAGGACA AGGAGCGCAG GCACCGCAGG 
AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC GTGTCAGGCC CCAACCTGTC GACCACACGG 
CCAATCCAGC AGGACCTGGG CCGCCAAGAC CCCCCGCTGG CCGAGGATAT CGACAACATG 
AAGAACAACA AACTGGCCAC TGCGGAGTCA GCCAGTCCCC ACGACAGCCT CAGCCACGCC 
GGCCTGCCCC AGAGCCCAGC CAAGATGGGG AACAGCATGG ACCCTGGCCC CACACCGGCC 
CCCACCACCA CGGCCACCAA CCCTCAGAAT GCCGTCAGTC GCCGGACAGC CAACAACCCG 
GGGAACCCGT CCAACCCCGG CCCCCCCAAG ACCCCTGAGA ACAGCCTTAT CGTCACCAAC 
CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG ACTGCCAGGA AACCTGACCA CACCACGGTG 
GACATCCCCC CAGCCTGCCC ACCCCCACTC AACCACACCA TCATCCAAGT GAACAAAAAC 
GCCAACCCAG ACCCACTGCC AAAAAAGGAG GAAGAGAAGA AAGAGGAGGA GGAAGACGAC 
CCCGGGGAAG ACGGCCCCAA GCCCATGCCC CCCTACAGCT CCATGTTTAT CCTCTCTACG 
ACCAACCCCC TTCGCCGCCT GTGCCATTAC ATCCTGAACC TGCGCTACTT TGAGATGTGC 
ATTCTCATGG TCATCGCCAT GAGCAGCATC GCCCTGGCGG CCGAGGATCC TGTTCAGCCC 
AACGCACCAC GAAACAATGT GCTGCGATAT TTTGACTACG TTTTTACAGG CGTCTTTACC 
TTTGAGATGG TGATCAAGAT GATCGACCTG GGGCTCGTCC TGCACCAGGG TGCCTACTTC 
CGTGACCTCT GGAACATTCT CGACTTCATA GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC 
TTCACGAGCA GTACACGTGG CAATAGCAAA GGAAAGGACA TCAACACGAT TAAATCCCTC 
CGAGTCCTCC GGGTGCTACG ACCTCTTAAA ACCATCAAGC GGTTGCCAAA GCTCAAGGCC 
GTGTTTGACT GCGTGGTGAA CTCTCTCAAG AATGTCTTCA ACATCCTCAT CGTCTACATG 
CTGTTCATGT TCATCTTCGC CGTGGTGGCC GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC 
TGCACAGATG AGTCCAAGGA GTTTGAGAAA GATTGCCGCG GCAAGTACCT CCTCTATGAG 
AAGAATGAGG TAAAGGCGAG GGATCGAGAG TGGAAGAAGT ATGAGTTCCA TTATGACAAT 
GTGCTGTGGG CGCTGCTGAC CCTCTTCACC GTGTCCACGG GAGAAGGCTG GCCACAGGTC 
CTCAAGCACT CCGTGGACGC CACCTTCGAG AACCAGGGCC CCAGCCCCGG GTACCGCATG 
GAGATGTCCA TCTTCTACGT CGTCTACTTT GTGGTGTTCC CCTTCTTCTT TGTCAACATC 
TTTGTGGCCT TGATCATCAT CACCTTCCAG GAGCAGGGGG ACAAGATGAT GGAGGAGTAC 
AGCCTGGAGA AAAATGAGAG GGCGTGCATC GACTTCGCCA TCAGCGCCAA ACCCCTGACC 
CGGCACATGC CGCAGAACAA GCAGAGTTTC CAGTACCGCA TGTGGCAGTT CGTGGTGTCC 
CCGCCTTTCG AGTACACCAT CATGGCCATG ATCGCCCTCA ACACCATCGT GCTCATGATG 
AAGTTCTACG GGGCCTCTGT TGCTTATGAG AATGCTCTGA GAGTATTCAA CATCGTCTTC 
ACCTCCCTCT TCTCACTGGA ATGTGTGCTG AAAGTCATGG CTTTTGGGAT TCTGAATTAT 
TTTCGCGATG CCTGGAACAT CTTCGACTTT GTGACTGTTC TGGGCAGCAT CACCGACATC 
CTCGTGACCG AGTTTGGGAA TAACTTCATC AACTTGAGCT TCCTCCGCCT CTTCCGAGCT 
GCCCGGCTCA TCAAACTCCT CCGTCAGGGT TACACCATCC GAATTCTTCT CTGGACCTTT 
GTGCAGTCCT TCAAGGCCCT GCCCTATGTC TGTCTACTGA TTGCCATGCT TTTTTTCATC 
TACGCCATCA TTGGGATGCA GGTGTTTGGC AACATTGGCA TCGATGTGGA GGACGAGGAC 
AGTGATGAAG ATGAATTCCA AATCACTGAG CACAATAACT TCCGCACCTT CTTCCAGGCC 
CTCATGCTTC TCTTCCGGAG TGCCACTGGG GAGGCTTGGC ACAACATCAT GCTTTCCTGC 
CTCAGCGGGA AACCCTGTGA TAAGAACTCT GGCATCCTGA CTCACGAATG CGGCAATGAA 
TTTGCTTATT TTTACTTCGT TTCCTTCATC TTCCTCTGCT CGTTTCTGAT GCTGAATCTT 
TTTGTTGCCG TCATCATGGA CAACTTTGAG TACCTCACCC GAGATTCCTC CATCCTGGGT 
CCCCACCACC TGGATGAGTA CGTGCGTGTC TGGGCCGAGT ATGACCCTGC AGCGTGCGGT 
CGGATTCATT ATAAGGATAT GTACAGTTTA TTGCGCGTAA TATCTCCCCC TCTCGGCTTA 
GGCAAGAAAT GTCCTCATAG GGTTGCTTGC AAGAGGCTCC TGCGGATGGA TCTGCCCGTC 
GCTGATGACA ACACCGTCCA CTTCAACTCT ACCCTCATGG CTCTGATCCG CACGGCCCTG 
GACATCAAAA TTGCCAAAGG TGGAGCTGAC AAACAACAAA TGGATGCTGA GTTGCGGAAG 
GAGATGATGG CCATCTGGCC CAATCTGTCC CAGAAGACGC TGGACCTGCT GGTCACCCCC 
CACAAGTCCA CGGACCTGAC AGTGGGGAAG ATCTATGCGG CCATGATGAT CATGGAGTAT 
TACCGGCAGA GCAAGGCCAA GAAGCTTCAG GCCATGCGCG AGGAACAGAA CCGGACACCT 
CTCATGTTCC AGCGCATGGA GCCCCCATCG CCAACACAGG AGGGGGGGCC TGGCCAGAAC 
GCCCTCCCCT CCACCCAGCT GGACCCAGGA GGAGGCTTGA TGGCTCACGA AAGTGGCATG 
AAAGAGAGCC CATCCTGGGT GACCCAGCGG GCCCAGGAGA TGTTCCAGAA GACGGGCACA 
TGGAGCCCAG AGCGGGGCCC CCCCACTGAT ATGCCTAATA GCCAACCCAA CTCTCAGTCT 
GTGGAGATGC GAGAGATGGG CAGAGACGGC TACTCTGACA GTGAGCACTA CTTCCCCGTT 
GAAGGCCAGG CCCGGGCCGC CTCCATGCCC CGCCTGCCTG CCGAGAACCA GAGGAGAAGG 
GGCCGGCCAC GTGGGAATAA CCTCAGTACC ATCTCAGACA CCAGCCCCAT GAAGCGCTCG 
GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC CTGGACGACT ACTCCCTGGA GAGGGTGCCG 
CCCGAGGAGA ACCAGCGGCA CCACCAGAGG CGCAGAGACC GTGGCCACCG CACCTCCGAG 
CGCTCCTTGG GCCGCTACAC GGACGTGGAC ACAGGCTTGG GGACAGACTT GAGCATGACC 
ACCCAGTCTG GGGACCTGCC ATCAAAGGAA AGAGACCAGG AGCGGGGCCG GCCCAAGGAC 
CGGAAGCATC GGCAACACCA CCACCACCAC CACCACCACC ATCCCCCGCC CCCTGACAAG 
GAGCGCTACA CCCAGGAGCG GCCTGAGCAC GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG 
TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC ATGGCCCACC GTCAGGGCAG TAGTTCCGTA 
AGTGGAAGCC CAGCCCCCTC AACATCTGGT ACCAGCACTC CGCGGCGGGG ACGCCGCCAG 
CTCCCCCAGA CCCCCTCCAC TCCCCGGCCG CACGTGTCCT ATTCCCCCGT GATCCGTAAG 
GCCGGCGGCT CGGGTCCCCA GCAGCAGCAG CAGCCCGCGG CGCGGCCGGG CCGAACGGCC 
CCCAGCGGCC CGCGGAGGTA CCCGGGCCCC GTGGCCGAGC CCCTGGCCGG GGAGCGGCCT 
CCCACGGGAG GCCACGGAAG CAGCCGCTCG CCCGCGATGG AGCGGCGGAT CCCGGGCCCG 
GCCCGGAGCG AGTCCCCCAG GGCGCCGTGT CGCCACGGCG GGGCGCGATG GCCGGCGTCC 
GGCGCGCACG CGGCCGAGGG CCCCCCGGGC CCCCGGCACC ACCACGGTTA CTACCGGGGC 
TCCGACTACG ACGAGCCAGA CGGCCCGGGC GGCGGCGGCG GGGAGGAGGA GGAGCCCCGG 
GCCGCCGCCT ACGACGCGCC GCCCCCCGCG CGCCACGGGT GCTCGCCCAG GACTCCCCGG 
GCCGCGGGCC CGGCCTGCGC CTCGCCTTCC AGGCACGGCC GGCGACTCCC GAACGGCTAC 
TACCCGGCCC ACGGACTGGC CAGGCCCCGC GGGCCGGGCT CCCGGAAAGG CCTGCACGAA 
GCCTACAGCG AGACCGACGA CGATGACTGG TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1A ( XM_024993859.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849627.1 Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X11, mRNA.

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CloneID	OBa235780
Clone ID Related Accession (Same CDS sequence)	XM_024993859.1
Accession Version	XM_024993859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA 
GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA 
ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG 
AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC 
GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA 
GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG 
TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC 
GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG 
AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT 
GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC 
GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC 
CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG 
ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG 
AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG 
AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT 
GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC 
AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT 
TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC 
TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC 
AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG 
CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG 
GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT 
GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC 
TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG 
TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC 
TGGGCCGAGT ATGACCCTGC AGCGTGCGGT CGGATTCATT ATAAGGATAT GTACAGTTTA 
TTGCGCGTAA TATCTCCCCC TCTCGGCTTA GGCAAGAAAT GTCCTCATAG GGTTGCTTGC 
AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT 
ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC 
AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC 
CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG 
ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG 
GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG 
CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA 
GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG 
GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT 
ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC 
TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC 
CGCCTGCCTG CCGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC 
ATCTCAGACA CCAGCCCCAT GAAGCGCTCG GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC 
CTGGACGACT ACTCCCTGGA GAGGGTGCCG CCCGAGGAGA ACCAGCGGCA CCACCAGAGG 
CGCAGAGACC GTGGCCACCG CACCTCCGAG CGCTCCTTGG GCCGCTACAC GGACGTGGAC 
ACAGGCTTGG GGACAGACTT GAGCATGACC ACCCAGTCTG GGGACCTGCC ATCAAAGGAA 
AGAGACCAGG AGCGGGGCCG GCCCAAGGAC CGGAAGCATC GGCAACACCA CCACCACCAC 
CACCACCACC ATCCCCCGCC CCCTGACAAG GAGCGCTACA CCCAGGAGCG GCCTGAGCAC 
GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC 
ATGGCCCACC GTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849627.1
CDS	230..7027
Translation	MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQRHPFDGALRRATIKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSSSVGLDTRRPWAGSQEAELSREGPYGRESDHHAREGGLEQPGFWEGEAERGKAGDPHRRHAHRQAGSRESRSGSPRTGADGEPRRHRAHRRPGDEAPDDKADRRSRHREGSRPARGGEGESEGPDGGGERRRRHRHGPPPAYDADMRREDKERRHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESASPHDSLSHAGLPQSPAKMGNSMDPGPTPAPTTTATNPQNAVSRRTANNPGNPSNPGPPKTPENSLIVTNPSSTQANSAKTARKPDHTTVDIPPACPPPLNHTIIQVNKNANPDPLPKKEEEKKEEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTHECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPGQNALPSTQLDPGGGLMAHESGMKESPSWVTQRAQEMFQKTGTWSPERGPPTDMPNSQPNSQSVEMREMGRDGYSDSEHYFPVEGQARAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRPKDRKHRQHHHHHHHHHPPPPDKERYTQERPEHGRPRARDQRWSRSPSEGREHMAHRQ

Target ORF information:

RefSeq Version	XM_024993859.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993859.1

ORF Insert Sequence:

ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG 
CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC 
CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC 
TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT 
GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC 
CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC 
ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT 
AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC 
ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG 
CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG 
AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT 
GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC 
ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC 
TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG 
TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG 
ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC 
ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT 
GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG 
CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA 
GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT 
CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG 
GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT 
GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC 
CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC 
ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC 
TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG 
CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC 
GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT 
GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC 
AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC 
CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT 
AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA 
GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT 
CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC 
GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC 
CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC 
CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT 
GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC 
GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA 
GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG 
GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG 
GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG 
AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC 
CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC 
GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG 
GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC 
CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG 
GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC 
CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC 
GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC 
AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC 
GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC 
CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA 
GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG 
AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT 
GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG 
ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG 
ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC 
AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG 
GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC 
CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC 
ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC 
GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT 
TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG 
GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA 
GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA 
GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA 
ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG 
AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC 
GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA 
GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG 
TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC 
GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG 
AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT 
GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC 
GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC 
CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG 
ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG 
AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG 
AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT 
GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC 
AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT 
TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC 
TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC 
AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG 
CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG 
GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT 
GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC 
TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG 
TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC 
TGGGCCGAGT ATGACCCTGC AGCGTGCGGT CGGATTCATT ATAAGGATAT GTACAGTTTA 
TTGCGCGTAA TATCTCCCCC TCTCGGCTTA GGCAAGAAAT GTCCTCATAG GGTTGCTTGC 
AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT 
ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC 
AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC 
CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG 
ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG 
GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG 
CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA 
GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG 
GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT 
ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC 
TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC 
CGCCTGCCTG CCGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC 
ATCTCAGACA CCAGCCCCAT GAAGCGCTCG GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC 
CTGGACGACT ACTCCCTGGA GAGGGTGCCG CCCGAGGAGA ACCAGCGGCA CCACCAGAGG 
CGCAGAGACC GTGGCCACCG CACCTCCGAG CGCTCCTTGG GCCGCTACAC GGACGTGGAC 
ACAGGCTTGG GGACAGACTT GAGCATGACC ACCCAGTCTG GGGACCTGCC ATCAAAGGAA 
AGAGACCAGG AGCGGGGCCG GCCCAAGGAC CGGAAGCATC GGCAACACCA CCACCACCAC 
CACCACCACC ATCCCCCGCC CCCTGACAAG GAGCGCTACA CCCAGGAGCG GCCTGAGCAC 
GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC 
ATGGCCCACC GTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CACNA1A
Entrez Gene ID	282648
Full Name	calcium voltage-gated channel subunit alpha1 A
Synonyms	CAV2.1
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 A Names voltage-dependent P/Q-type calcium channel subunit alpha-1A calcium channel, alpha 1A subunit calcium channel, voltage-dependent, P/Q type, alpha 1A subunit voltage-gated calcium channel alpha 1A subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	CACNA1A	NP_001120694.1
Pan troglodytes (chimpanzee)	CACNA1A	XP_003316202.1
Xenopus tropicalis (tropical clawed frog)	cacna1a	XP_004919196.1
Macaca mulatta (Rhesus monkey)	CACNA1A	XP_002801141.1
Danio rerio (zebrafish)	cacna1ab	XP_698014.6
Bos taurus (cattle)	CACNA1A	NP_001068597.1
Mus musculus (house mouse)	Cacna1a	NP_031604.3
Rattus norvegicus (Norway rat)	Cacna1a	NP_037050.2
Canis lupus familiaris (dog)	CACNA1A	XP_005632867.1

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CACNA1A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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