ATP6V1A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP6V1A
Entrez Gene ID	282147
Full Name	ATPase H+ transporting V1 subunit A
Synonyms	ATP6A1
General protein information	Preferred Names ATPase H+ transporting V1 subunit A Names V-type proton ATPase catalytic subunit A ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha polypeptide, 70kD ATPase, H+ transporting, lysosomal V1 subunit A H(+)-ATPase subunit A V-ATPase 69 kDa subunit V-ATPase subunit A vacuolar H+-ATPase A subunit vacuolar proton pump subunit alpha
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015470992.1	XP_015326478.1	V-type proton ATPase catalytic subunit A isoform X1
XM_015470992.2	XP_015326478.1	V-type proton ATPase catalytic subunit A isoform X1
NM_174504.2	NP_776929.1	V-type proton ATPase catalytic subunit A

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F05401g	XP_455325.1
Oryza sativa (rice)	Os02g0175400	NP_001046054.1
Canis lupus familiaris (dog)	ATP6V1A	XP_545103.1
Bos taurus (cattle)	ATP6V1A	NP_776929.1
Mus musculus (house mouse)	Atp6v1a	NP_031534.2
Arabidopsis thaliana (thale cress)	VHA-A	NP_178011.1
Schizosaccharomyces pombe (fission yeast)	vma1	NP_593425.1
Neurospora crassa	NCU01207	XP_961573.1
Homo sapiens (human)	ATP6V1A	NP_001681.2
Danio rerio (zebrafish)	atp6v1ab	XP_002666686.1
Danio rerio (zebrafish)	atp6v1aa	NP_957429.1
Magnaporthe oryzae (rice blast fungus)	MGG_08087	XP_003715042.1
Macaca mulatta (Rhesus monkey)	ATP6V1A	XP_001106889.1
Gallus gallus (chicken)	ATP6V1A	NP_990305.1
Caenorhabditis elegans (roundworm)	vha-13	NP_506559.1
Pan troglodytes (chimpanzee)	ATP6V1A	XP_003309977.1
Macaca mulatta (Rhesus monkey)	LOC696878	XP_001091043.1
Rattus norvegicus (Norway rat)	Atp6v1a	NP_001101788.1
Oryza sativa (rice)	Os06g0662000	NP_001058280.1
Drosophila melanogaster (fruit fly)	Vha68-1	NP_523560.2
Saccharomyces cerevisiae (baker's yeast)	VMA1	NP_010096.1
Xenopus tropicalis (tropical clawed frog)	atp6v1a	NP_001007512.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

[Structural peculiarities of Na+,K+-ATPase isozymes from the calf brain].
Vladimirova NM, Sautkina EN, Murav'eva TI, Ovchinnikova TV, Potapenko NA
Bioorganicheskaia khimiia29(2)146-58(2003 Mar-Apr)

cDNA sequence and tissue expression of bovine vacuolar H(+)-ATPase M(r) 70,000 subunit.
Marushack MM, Lee BS, Masood K, Gluck S
The American journal of physiology263(1 Pt 2)F171-4(1992 Jul)

A single gene encodes the catalytic "A" subunit of the bovine vacuolar H(+)-ATPase.
Puopolo K, Kumamoto C, Adachi I, Forgac M
The Journal of biological chemistry266(36)24564-72(1991 Dec)

Structure and expression of subunit A from the bovine chromaffin cell vacuolar ATPase.
Pan YX, Xu J, Strasser JE, Howell M, Dean GE
FEBS letters293(1-2)89-92(1991 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ATP6V1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP6V1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa15578	NM_174504.2 Latest version!	Bos taurus ATPase H+ transporting V1 subunit A (ATP6V1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa15578	XM_015470992.2 Latest version!	Bos taurus ATPase H+ transporting V1 subunit A (ATP6V1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OBa15578	XM_015470992.1	Bos taurus ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A (ATP6V1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP6V1A ( NM_174504.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776929.1 Bos taurus ATPase H+ transporting V1 subunit A (ATP6V1A), mRNA.

Price & Availability↑

CloneID	OBa15578
Clone ID Related Accession (Same CDS sequence)	XM_015470992.1 , XM_015470992.2 , NM_174504.2
Accession Version	NM_174504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Bos taurus(cattle)
Product	V-type proton ATPase catalytic subunit A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105145.1. On Sep 25, 2005 this sequence version replaced NM_174504.1. ##Evidence-Data-START## Transcript exon combination :: BC105145.1, X58386.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGATGGATT TCTCCAAGCT CCCCAAAATA CGTGATGAAG ATAAAGAAAG CACGTTTGGT 
TATGTGCATG GGGTCTCAGG ACCTGTGGTT ACCGCCTGTG ACATGGCAGG CGCAGCCATG 
TACGAGCTGG TGAGAGTGGG CCACAGCGAG TTGGTTGGAG AGATTATCCG ATTAGAGGGC 
GACATGGCTA CTATCCAGGT GTATGAAGAA ACTTCTGGTG TGTCTGTTGG AGACCCTGTA 
CTTCGCACTG GTAAACCCCT CTCAGTTGAG CTTGGTCCAG GCATTATGGG AGCCATTTTT 
GATGGTATTC AAAGACCTTT GTCGGATATC AGCAGTCAGA CTCAAAGTAT TTACATTCCC 
AGAGGAGTAA ATGTGTCTGC TCTTAGCAGA GATGTCAAAT GGGATTTCAC ACCTTGCAAA 
AACCTACGGG TTGGTAGTCA CATCACTGGT GGAGATATTT ACGGAATTGT CAATGAGAAC 
TCTCTCATCA AACACAAAAT CATGTTGCCT CCACGAAACA GAGGAACTGT CACCTACATC 
GCTCCACCTG GAAATTACGA TACCTCTGAT GTCGTGTTGG AGCTTGAATT TGAAGGTATA 
AAGGAGAAGT TCAGCATGGT CCAAGTATGG CCTGTACGTC AGGTTCGGCC TGTCACTGAG 
AAGTTGCCAG CTAATCATCC TCTGTTGACT GGCCAGAGAG TCCTTGATGC CCTTTTTCCA 
TGTGTACAGG GAGGAACTAC TGCAATCCCT GGGGCCTTTG GCTGTGGGAA GACAGTGATA 
TCACAGTCTC TATCCAAGTA TTCCAACAGT GATGTGATCA TCTACGTAGG ATGTGGTGAG 
AGAGGAAACG AGATGTCTGA AGTCCTCCGG GACTTCCCAG AGCTCACGAT GGAAGTCGAT 
GGTAAGGTAG AGTCAATTAT GAAGAGAACA GCATTGGTAG CCAATACATC CAATATGCCT 
GTGGCTGCTA GAGAAGCCTC TATTTATACT GGAATTACAC TGTCAGAATA TTTCCGTGAC 
ATGGGCTATC ATGTCAGTAT GATGGCTGAC TCCACCTCTC GATGGGCTGA GGCTCTTAGA 
GAAATCTCTG GTCGTTTGGC TGAAATGCCT GCAGATAGTG GATATCCTGC ATATCTTGGT 
GCCCGTTTGG CCTCTTTCTA TGAGCGAGCC GGCAGAGTGA AGTGTCTTGG AAATCCTGAG 
AGGGAAGGGA GTGTCAGCAT TGTAGGAGCA GTTTCTCCAC CTGGTGGTGA CTTTTCTGAT 
CCAGTTACAT CTGCTACTCT TGGTATTGTT CAGGTGTTCT GGGGCTTAGA TAAGAAACTA 
GCTCAACGTA AGCATTTCCC TTCTGTCAAT TGGCTGATCA GCTACAGCAA GTACATGCGT 
GCCTTGGATG AGTACTACGA CAAACACTTC ACAGAGTTCG TCCCTCTGAG GACCAAAGCT 
AAGGAGATTC TGCAGGAAGA AGAAGACTTG GCAGAAATCG TACAGCTGGT GGGAAAGGCT 
TCTCTAGCAG AAACAGATAA AATCACTCTG GAGGTAGCAA AACTTATCAA AGATGATTTC 
CTTCAACAAA ATGGATATAC TCCTTATGAC AGGTTCTGCC CATTCTACAA GACAGTGGGG 
ATGCTGTCCA ACATGATTGC ATTTTATGAT ATGGCCCGCA GAGCTGTTGA AACCACTGCC 
CAGAGTGACA ATAAAATCAC ATGGTCCATT ATCCGTGAGC ACATGGGGGA GATCCTCTAT 
AAGCTTTCCT CCATGAAATT CAAGGATCCA GTGAAAGATG GTGAGGCGAA GATCAAGGCC 
GACTATGCAC AACTTCTTGA AGATATGCAG AATGCATTCC GTAGCCTTGA AGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776929.1
CDS	57..1913
Translation	MMDFSKLPKIRDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDVKWDFTPCKNLRVGSHITGGDIYGIVNENSLIKHKIMLPPRNRGTVTYIAPPGNYDTSDVVLELEFEGIKEKFSMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGEILYKLSSMKFKDPVKDGEAKIKADYAQLLEDMQNAFRSLED

Target ORF information:

RefSeq Version	NM_174504.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ATPase H+ transporting V1 subunit A (ATP6V1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174504.2

ORF Insert Sequence:

ATGATGGATT TCTCCAAGCT CCCCAAAATA CGTGATGAAG ATAAAGAAAG CACGTTTGGT 
TATGTGCATG GGGTCTCAGG ACCTGTGGTT ACCGCCTGTG ACATGGCAGG CGCAGCCATG 
TACGAGCTGG TGAGAGTGGG CCACAGCGAG TTGGTTGGAG AGATTATCCG ATTAGAGGGC 
GACATGGCTA CTATCCAGGT GTATGAAGAA ACTTCTGGTG TGTCTGTTGG AGACCCTGTA 
CTTCGCACTG GTAAACCCCT CTCAGTTGAG CTTGGTCCAG GCATTATGGG AGCCATTTTT 
GATGGTATTC AAAGACCTTT GTCGGATATC AGCAGTCAGA CTCAAAGTAT TTACATTCCC 
AGAGGAGTAA ATGTGTCTGC TCTTAGCAGA GATGTCAAAT GGGATTTCAC ACCTTGCAAA 
AACCTACGGG TTGGTAGTCA CATCACTGGT GGAGATATTT ACGGAATTGT CAATGAGAAC 
TCTCTCATCA AACACAAAAT CATGTTGCCT CCACGAAACA GAGGAACTGT CACCTACATC 
GCTCCACCTG GAAATTACGA TACCTCTGAT GTCGTGTTGG AGCTTGAATT TGAAGGTATA 
AAGGAGAAGT TCAGCATGGT CCAAGTATGG CCTGTACGTC AGGTTCGGCC TGTCACTGAG 
AAGTTGCCAG CTAATCATCC TCTGTTGACT GGCCAGAGAG TCCTTGATGC CCTTTTTCCA 
TGTGTACAGG GAGGAACTAC TGCAATCCCT GGGGCCTTTG GCTGTGGGAA GACAGTGATA 
TCACAGTCTC TATCCAAGTA TTCCAACAGT GATGTGATCA TCTACGTAGG ATGTGGTGAG 
AGAGGAAACG AGATGTCTGA AGTCCTCCGG GACTTCCCAG AGCTCACGAT GGAAGTCGAT 
GGTAAGGTAG AGTCAATTAT GAAGAGAACA GCATTGGTAG CCAATACATC CAATATGCCT 
GTGGCTGCTA GAGAAGCCTC TATTTATACT GGAATTACAC TGTCAGAATA TTTCCGTGAC 
ATGGGCTATC ATGTCAGTAT GATGGCTGAC TCCACCTCTC GATGGGCTGA GGCTCTTAGA 
GAAATCTCTG GTCGTTTGGC TGAAATGCCT GCAGATAGTG GATATCCTGC ATATCTTGGT 
GCCCGTTTGG CCTCTTTCTA TGAGCGAGCC GGCAGAGTGA AGTGTCTTGG AAATCCTGAG 
AGGGAAGGGA GTGTCAGCAT TGTAGGAGCA GTTTCTCCAC CTGGTGGTGA CTTTTCTGAT 
CCAGTTACAT CTGCTACTCT TGGTATTGTT CAGGTGTTCT GGGGCTTAGA TAAGAAACTA 
GCTCAACGTA AGCATTTCCC TTCTGTCAAT TGGCTGATCA GCTACAGCAA GTACATGCGT 
GCCTTGGATG AGTACTACGA CAAACACTTC ACAGAGTTCG TCCCTCTGAG GACCAAAGCT 
AAGGAGATTC TGCAGGAAGA AGAAGACTTG GCAGAAATCG TACAGCTGGT GGGAAAGGCT 
TCTCTAGCAG AAACAGATAA AATCACTCTG GAGGTAGCAA AACTTATCAA AGATGATTTC 
CTTCAACAAA ATGGATATAC TCCTTATGAC AGGTTCTGCC CATTCTACAA GACAGTGGGG 
ATGCTGTCCA ACATGATTGC ATTTTATGAT ATGGCCCGCA GAGCTGTTGA AACCACTGCC 
CAGAGTGACA ATAAAATCAC ATGGTCCATT ATCCGTGAGC ACATGGGGGA GATCCTCTAT 
AAGCTTTCCT CCATGAAATT CAAGGATCCA GTGAAAGATG GTGAGGCGAA GATCAAGGCC 
GACTATGCAC AACTTCTTGA AGATATGCAG AATGCATTCC GTAGCCTTGA AGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V1A ( XM_015470992.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326478.1 Bos taurus ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A (ATP6V1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa15578
Clone ID Related Accession (Same CDS sequence)	XM_015470992.1 , XM_015470992.2 , NM_174504.2
Accession Version	XM_015470992.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	V-type proton ATPase catalytic subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATT TCTCCAAGCT CCCCAAAATA CGTGATGAAG ATAAAGAAAG CACGTTTGGT 
TATGTGCATG GGGTCTCAGG ACCTGTGGTT ACCGCCTGTG ACATGGCAGG CGCAGCCATG 
TACGAGCTGG TGAGAGTGGG CCACAGCGAG TTGGTTGGAG AGATTATCCG ATTAGAGGGC 
GACATGGCTA CTATCCAGGT GTATGAAGAA ACTTCTGGTG TGTCTGTTGG AGACCCTGTA 
CTTCGCACTG GTAAACCCCT CTCAGTTGAG CTTGGTCCAG GCATTATGGG AGCCATTTTT 
GATGGTATTC AAAGACCTTT GTCGGATATC AGCAGTCAGA CTCAAAGTAT TTACATTCCC 
AGAGGAGTAA ATGTGTCTGC TCTTAGCAGA GATGTCAAAT GGGATTTCAC ACCTTGCAAA 
AACCTACGGG TTGGTAGTCA CATCACTGGT GGAGATATTT ACGGAATTGT CAATGAGAAC 
TCTCTCATCA AACACAAAAT CATGTTGCCT CCACGAAACA GAGGAACTGT CACCTACATC 
GCTCCACCTG GAAATTACGA TACCTCTGAT GTCGTGTTGG AGCTTGAATT TGAAGGTATA 
AAGGAGAAGT TCAGCATGGT CCAAGTATGG CCTGTACGTC AGGTTCGGCC TGTCACTGAG 
AAGTTGCCAG CTAATCATCC TCTGTTGACT GGCCAGAGAG TCCTTGATGC CCTTTTTCCA 
TGTGTACAGG GAGGAACTAC TGCAATCCCT GGGGCCTTTG GCTGTGGGAA GACAGTGATA 
TCACAGTCTC TATCCAAGTA TTCCAACAGT GATGTGATCA TCTACGTAGG ATGTGGTGAG 
AGAGGAAACG AGATGTCTGA AGTCCTCCGG GACTTCCCAG AGCTCACGAT GGAAGTCGAT 
GGTAAGGTAG AGTCAATTAT GAAGAGAACA GCATTGGTAG CCAATACATC CAATATGCCT 
GTGGCTGCTA GAGAAGCCTC TATTTATACT GGAATTACAC TGTCAGAATA TTTCCGTGAC 
ATGGGCTATC ATGTCAGTAT GATGGCTGAC TCCACCTCTC GATGGGCTGA GGCTCTTAGA 
GAAATCTCTG GTCGTTTGGC TGAAATGCCT GCAGATAGTG GATATCCTGC ATATCTTGGT 
GCCCGTTTGG CCTCTTTCTA TGAGCGAGCC GGCAGAGTGA AGTGTCTTGG AAATCCTGAG 
AGGGAAGGGA GTGTCAGCAT TGTAGGAGCA GTTTCTCCAC CTGGTGGTGA CTTTTCTGAT 
CCAGTTACAT CTGCTACTCT TGGTATTGTT CAGGTGTTCT GGGGCTTAGA TAAGAAACTA 
GCTCAACGTA AGCATTTCCC TTCTGTCAAT TGGCTGATCA GCTACAGCAA GTACATGCGT 
GCCTTGGATG AGTACTACGA CAAACACTTC ACAGAGTTCG TCCCTCTGAG GACCAAAGCT 
AAGGAGATTC TGCAGGAAGA AGAAGACTTG GCAGAAATCG TACAGCTGGT GGGAAAGGCT 
TCTCTAGCAG AAACAGATAA AATCACTCTG GAGGTAGCAA AACTTATCAA AGATGATTTC 
CTTCAACAAA ATGGATATAC TCCTTATGAC AGGTTCTGCC CATTCTACAA GACAGTGGGG 
ATGCTGTCCA ACATGATTGC ATTTTATGAT ATGGCCCGCA GAGCTGTTGA AACCACTGCC 
CAGAGTGACA ATAAAATCAC ATGGTCCATT ATCCGTGAGC ACATGGGGGA GATCCTCTAT 
AAGCTTTCCT CCATGAAATT CAAGGATCCA GTGAAAGATG GTGAGGCGAA GATCAAGGCC 
GACTATGCAC AACTTCTTGA AGATATGCAG AATGCATTCC GTAGCCTTGA AGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326478.1
CDS	319..2175
Translation	MMDFSKLPKIRDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDVKWDFTPCKNLRVGSHITGGDIYGIVNENSLIKHKIMLPPRNRGTVTYIAPPGNYDTSDVVLELEFEGIKEKFSMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGEILYKLSSMKFKDPVKDGEAKIKADYAQLLEDMQNAFRSLED

Target ORF information:

RefSeq Version	XM_015470992.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A (ATP6V1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470992.1

ORF Insert Sequence:

ATGATGGATT TCTCCAAGCT CCCCAAAATA CGTGATGAAG ATAAAGAAAG CACGTTTGGT 
TATGTGCATG GGGTCTCAGG ACCTGTGGTT ACCGCCTGTG ACATGGCAGG CGCAGCCATG 
TACGAGCTGG TGAGAGTGGG CCACAGCGAG TTGGTTGGAG AGATTATCCG ATTAGAGGGC 
GACATGGCTA CTATCCAGGT GTATGAAGAA ACTTCTGGTG TGTCTGTTGG AGACCCTGTA 
CTTCGCACTG GTAAACCCCT CTCAGTTGAG CTTGGTCCAG GCATTATGGG AGCCATTTTT 
GATGGTATTC AAAGACCTTT GTCGGATATC AGCAGTCAGA CTCAAAGTAT TTACATTCCC 
AGAGGAGTAA ATGTGTCTGC TCTTAGCAGA GATGTCAAAT GGGATTTCAC ACCTTGCAAA 
AACCTACGGG TTGGTAGTCA CATCACTGGT GGAGATATTT ACGGAATTGT CAATGAGAAC 
TCTCTCATCA AACACAAAAT CATGTTGCCT CCACGAAACA GAGGAACTGT CACCTACATC 
GCTCCACCTG GAAATTACGA TACCTCTGAT GTCGTGTTGG AGCTTGAATT TGAAGGTATA 
AAGGAGAAGT TCAGCATGGT CCAAGTATGG CCTGTACGTC AGGTTCGGCC TGTCACTGAG 
AAGTTGCCAG CTAATCATCC TCTGTTGACT GGCCAGAGAG TCCTTGATGC CCTTTTTCCA 
TGTGTACAGG GAGGAACTAC TGCAATCCCT GGGGCCTTTG GCTGTGGGAA GACAGTGATA 
TCACAGTCTC TATCCAAGTA TTCCAACAGT GATGTGATCA TCTACGTAGG ATGTGGTGAG 
AGAGGAAACG AGATGTCTGA AGTCCTCCGG GACTTCCCAG AGCTCACGAT GGAAGTCGAT 
GGTAAGGTAG AGTCAATTAT GAAGAGAACA GCATTGGTAG CCAATACATC CAATATGCCT 
GTGGCTGCTA GAGAAGCCTC TATTTATACT GGAATTACAC TGTCAGAATA TTTCCGTGAC 
ATGGGCTATC ATGTCAGTAT GATGGCTGAC TCCACCTCTC GATGGGCTGA GGCTCTTAGA 
GAAATCTCTG GTCGTTTGGC TGAAATGCCT GCAGATAGTG GATATCCTGC ATATCTTGGT 
GCCCGTTTGG CCTCTTTCTA TGAGCGAGCC GGCAGAGTGA AGTGTCTTGG AAATCCTGAG 
AGGGAAGGGA GTGTCAGCAT TGTAGGAGCA GTTTCTCCAC CTGGTGGTGA CTTTTCTGAT 
CCAGTTACAT CTGCTACTCT TGGTATTGTT CAGGTGTTCT GGGGCTTAGA TAAGAAACTA 
GCTCAACGTA AGCATTTCCC TTCTGTCAAT TGGCTGATCA GCTACAGCAA GTACATGCGT 
GCCTTGGATG AGTACTACGA CAAACACTTC ACAGAGTTCG TCCCTCTGAG GACCAAAGCT 
AAGGAGATTC TGCAGGAAGA AGAAGACTTG GCAGAAATCG TACAGCTGGT GGGAAAGGCT 
TCTCTAGCAG AAACAGATAA AATCACTCTG GAGGTAGCAA AACTTATCAA AGATGATTTC 
CTTCAACAAA ATGGATATAC TCCTTATGAC AGGTTCTGCC CATTCTACAA GACAGTGGGG 
ATGCTGTCCA ACATGATTGC ATTTTATGAT ATGGCCCGCA GAGCTGTTGA AACCACTGCC 
CAGAGTGACA ATAAAATCAC ATGGTCCATT ATCCGTGAGC ACATGGGGGA GATCCTCTAT 
AAGCTTTCCT CCATGAAATT CAAGGATCCA GTGAAAGATG GTGAGGCGAA GATCAAGGCC 
GACTATGCAC AACTTCTTGA AGATATGCAG AATGCATTCC GTAGCCTTGA AGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V1A ( XM_015470992.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326478.1 Bos taurus ATPase H+ transporting V1 subunit A (ATP6V1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa15578
Clone ID Related Accession (Same CDS sequence)	XM_015470992.1 , XM_015470992.2 , NM_174504.2
Accession Version	XM_015470992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	V-type proton ATPase catalytic subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATT TCTCCAAGCT CCCCAAAATA CGTGATGAAG ATAAAGAAAG CACGTTTGGT 
TATGTGCATG GGGTCTCAGG ACCTGTGGTT ACCGCCTGTG ACATGGCAGG CGCAGCCATG 
TACGAGCTGG TGAGAGTGGG CCACAGCGAG TTGGTTGGAG AGATTATCCG ATTAGAGGGC 
GACATGGCTA CTATCCAGGT GTATGAAGAA ACTTCTGGTG TGTCTGTTGG AGACCCTGTA 
CTTCGCACTG GTAAACCCCT CTCAGTTGAG CTTGGTCCAG GCATTATGGG AGCCATTTTT 
GATGGTATTC AAAGACCTTT GTCGGATATC AGCAGTCAGA CTCAAAGTAT TTACATTCCC 
AGAGGAGTAA ATGTGTCTGC TCTTAGCAGA GATGTCAAAT GGGATTTCAC ACCTTGCAAA 
AACCTACGGG TTGGTAGTCA CATCACTGGT GGAGATATTT ACGGAATTGT CAATGAGAAC 
TCTCTCATCA AACACAAAAT CATGTTGCCT CCACGAAACA GAGGAACTGT CACCTACATC 
GCTCCACCTG GAAATTACGA TACCTCTGAT GTCGTGTTGG AGCTTGAATT TGAAGGTATA 
AAGGAGAAGT TCAGCATGGT CCAAGTATGG CCTGTACGTC AGGTTCGGCC TGTCACTGAG 
AAGTTGCCAG CTAATCATCC TCTGTTGACT GGCCAGAGAG TCCTTGATGC CCTTTTTCCA 
TGTGTACAGG GAGGAACTAC TGCAATCCCT GGGGCCTTTG GCTGTGGGAA GACAGTGATA 
TCACAGTCTC TATCCAAGTA TTCCAACAGT GATGTGATCA TCTACGTAGG ATGTGGTGAG 
AGAGGAAACG AGATGTCTGA AGTCCTCCGG GACTTCCCAG AGCTCACGAT GGAAGTCGAT 
GGTAAGGTAG AGTCAATTAT GAAGAGAACA GCATTGGTAG CCAATACATC CAATATGCCT 
GTGGCTGCTA GAGAAGCCTC TATTTATACT GGAATTACAC TGTCAGAATA TTTCCGTGAC 
ATGGGCTATC ATGTCAGTAT GATGGCTGAC TCCACCTCTC GATGGGCTGA GGCTCTTAGA 
GAAATCTCTG GTCGTTTGGC TGAAATGCCT GCAGATAGTG GATATCCTGC ATATCTTGGT 
GCCCGTTTGG CCTCTTTCTA TGAGCGAGCC GGCAGAGTGA AGTGTCTTGG AAATCCTGAG 
AGGGAAGGGA GTGTCAGCAT TGTAGGAGCA GTTTCTCCAC CTGGTGGTGA CTTTTCTGAT 
CCAGTTACAT CTGCTACTCT TGGTATTGTT CAGGTGTTCT GGGGCTTAGA TAAGAAACTA 
GCTCAACGTA AGCATTTCCC TTCTGTCAAT TGGCTGATCA GCTACAGCAA GTACATGCGT 
GCCTTGGATG AGTACTACGA CAAACACTTC ACAGAGTTCG TCCCTCTGAG GACCAAAGCT 
AAGGAGATTC TGCAGGAAGA AGAAGACTTG GCAGAAATCG TACAGCTGGT GGGAAAGGCT 
TCTCTAGCAG AAACAGATAA AATCACTCTG GAGGTAGCAA AACTTATCAA AGATGATTTC 
CTTCAACAAA ATGGATATAC TCCTTATGAC AGGTTCTGCC CATTCTACAA GACAGTGGGG 
ATGCTGTCCA ACATGATTGC ATTTTATGAT ATGGCCCGCA GAGCTGTTGA AACCACTGCC 
CAGAGTGACA ATAAAATCAC ATGGTCCATT ATCCGTGAGC ACATGGGGGA GATCCTCTAT 
AAGCTTTCCT CCATGAAATT CAAGGATCCA GTGAAAGATG GTGAGGCGAA GATCAAGGCC 
GACTATGCAC AACTTCTTGA AGATATGCAG AATGCATTCC GTAGCCTTGA AGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326478.1
CDS	351..2207
Translation	MMDFSKLPKIRDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDVKWDFTPCKNLRVGSHITGGDIYGIVNENSLIKHKIMLPPRNRGTVTYIAPPGNYDTSDVVLELEFEGIKEKFSMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGEILYKLSSMKFKDPVKDGEAKIKADYAQLLEDMQNAFRSLED

Target ORF information:

RefSeq Version	XM_015470992.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ATPase H+ transporting V1 subunit A (ATP6V1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470992.2

ORF Insert Sequence:

ATGATGGATT TCTCCAAGCT CCCCAAAATA CGTGATGAAG ATAAAGAAAG CACGTTTGGT 
TATGTGCATG GGGTCTCAGG ACCTGTGGTT ACCGCCTGTG ACATGGCAGG CGCAGCCATG 
TACGAGCTGG TGAGAGTGGG CCACAGCGAG TTGGTTGGAG AGATTATCCG ATTAGAGGGC 
GACATGGCTA CTATCCAGGT GTATGAAGAA ACTTCTGGTG TGTCTGTTGG AGACCCTGTA 
CTTCGCACTG GTAAACCCCT CTCAGTTGAG CTTGGTCCAG GCATTATGGG AGCCATTTTT 
GATGGTATTC AAAGACCTTT GTCGGATATC AGCAGTCAGA CTCAAAGTAT TTACATTCCC 
AGAGGAGTAA ATGTGTCTGC TCTTAGCAGA GATGTCAAAT GGGATTTCAC ACCTTGCAAA 
AACCTACGGG TTGGTAGTCA CATCACTGGT GGAGATATTT ACGGAATTGT CAATGAGAAC 
TCTCTCATCA AACACAAAAT CATGTTGCCT CCACGAAACA GAGGAACTGT CACCTACATC 
GCTCCACCTG GAAATTACGA TACCTCTGAT GTCGTGTTGG AGCTTGAATT TGAAGGTATA 
AAGGAGAAGT TCAGCATGGT CCAAGTATGG CCTGTACGTC AGGTTCGGCC TGTCACTGAG 
AAGTTGCCAG CTAATCATCC TCTGTTGACT GGCCAGAGAG TCCTTGATGC CCTTTTTCCA 
TGTGTACAGG GAGGAACTAC TGCAATCCCT GGGGCCTTTG GCTGTGGGAA GACAGTGATA 
TCACAGTCTC TATCCAAGTA TTCCAACAGT GATGTGATCA TCTACGTAGG ATGTGGTGAG 
AGAGGAAACG AGATGTCTGA AGTCCTCCGG GACTTCCCAG AGCTCACGAT GGAAGTCGAT 
GGTAAGGTAG AGTCAATTAT GAAGAGAACA GCATTGGTAG CCAATACATC CAATATGCCT 
GTGGCTGCTA GAGAAGCCTC TATTTATACT GGAATTACAC TGTCAGAATA TTTCCGTGAC 
ATGGGCTATC ATGTCAGTAT GATGGCTGAC TCCACCTCTC GATGGGCTGA GGCTCTTAGA 
GAAATCTCTG GTCGTTTGGC TGAAATGCCT GCAGATAGTG GATATCCTGC ATATCTTGGT 
GCCCGTTTGG CCTCTTTCTA TGAGCGAGCC GGCAGAGTGA AGTGTCTTGG AAATCCTGAG 
AGGGAAGGGA GTGTCAGCAT TGTAGGAGCA GTTTCTCCAC CTGGTGGTGA CTTTTCTGAT 
CCAGTTACAT CTGCTACTCT TGGTATTGTT CAGGTGTTCT GGGGCTTAGA TAAGAAACTA 
GCTCAACGTA AGCATTTCCC TTCTGTCAAT TGGCTGATCA GCTACAGCAA GTACATGCGT 
GCCTTGGATG AGTACTACGA CAAACACTTC ACAGAGTTCG TCCCTCTGAG GACCAAAGCT 
AAGGAGATTC TGCAGGAAGA AGAAGACTTG GCAGAAATCG TACAGCTGGT GGGAAAGGCT 
TCTCTAGCAG AAACAGATAA AATCACTCTG GAGGTAGCAA AACTTATCAA AGATGATTTC 
CTTCAACAAA ATGGATATAC TCCTTATGAC AGGTTCTGCC CATTCTACAA GACAGTGGGG 
ATGCTGTCCA ACATGATTGC ATTTTATGAT ATGGCCCGCA GAGCTGTTGA AACCACTGCC 
CAGAGTGACA ATAAAATCAC ATGGTCCATT ATCCGTGAGC ACATGGGGGA GATCCTCTAT 
AAGCTTTCCT CCATGAAATT CAAGGATCCA GTGAAAGATG GTGAGGCGAA GATCAAGGCC 
GACTATGCAC AACTTCTTGA AGATATGCAG AATGCATTCC GTAGCCTTGA AGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

[Structural peculiarities of Na+,K+-ATPase isozymes from the calf brain].
Bioorganicheskaia khimiia29(2)146-58(2003 Mar-Apr)
Vladimirova NM,Sautkina EN,Murav'eva TI,Ovchinnikova TV,Potapenko NA

cDNA sequence and tissue expression of bovine vacuolar H(+)-ATPase M(r) 70,000 subunit.
The American journal of physiology263(1 Pt 2)F171-4(1992 Jul)
Marushack MM,Lee BS,Masood K,Gluck S

A single gene encodes the catalytic "A" subunit of the bovine vacuolar H(+)-ATPase.
The Journal of biological chemistry266(36)24564-72(1991 Dec)
Puopolo K,Kumamoto C,Adachi I,Forgac M

Structure and expression of subunit A from the bovine chromaffin cell vacuolar ATPase.
FEBS letters293(1-2)89-92(1991 Nov)
Pan YX,Xu J,Strasser JE,Howell M,Dean GE

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ATP6V1A cDNA ORF clone, Bos taurus(cattle)

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Clones

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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