| Gene Symbol | APLN |
| Entrez Gene ID | 282143 |
| Full Name | apelin |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Bos taurus(cattle) |
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APLN cDNA ORF clone, Bos taurus(cattle)
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Gene
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Clones
gRNAs
The following APLN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APLN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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Reference Sequences (Refseq)
CloneID OBa35135 Clone ID Related Accession (Same CDS sequence) XM_010821394.3 , NM_174503.1 , XM_005227485.3 , XM_005227485.4 , XM_010821394.2 Accession Version NM_174503.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 234bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector 
User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1577116800000 Organism Bos taurus(cattle) Product apelin precursor Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB023492.1. ##Evidence-Data-START## Transcript exon combination :: AB023492.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##
1
61
121
181ATGAATCTGC GGCGCTGCGT GCAGGCGCTC CTGCTGCTCT GGCTCTGCCT GAGCGCGGTG
TGCGGAGGAC CCCTGCTGCA GACTTCTGAC GGGAAGGAGA TGGAAGAAGG CACCATCCGA
TACCTGGTGC AGCCCAGGGG GCCGAGGAGC GGCCCAGGCC CCTGGCAGGG AGGTCGGAGG
AAGTTCCGGC GCCAGCGGCC ACGCCTCTCC CACAAGGGTC CCATGCCTTT CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_776928.1 CDS 1..234 Translation 
Target ORF information:
RefSeq Version NM_174503.1 Organism Bos taurus(cattle) Definition Bos taurus apelin (APLN), mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK 
NM_174503.1
ORF Insert Sequence:
1
61
121
181ATGAATCTGC GGCGCTGCGT GCAGGCGCTC CTGCTGCTCT GGCTCTGCCT GAGCGCGGTG
TGCGGAGGAC CCCTGCTGCA GACTTCTGAC GGGAAGGAGA TGGAAGAAGG CACCATCCGA
TACCTGGTGC AGCCCAGGGG GCCGAGGAGC GGCCCAGGCC CCTGGCAGGG AGGTCGGAGG
AAGTTCCGGC GCCAGCGGCC ACGCCTCTCC CACAAGGGTC CCATGCCTTT CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OBa35135 Clone ID Related Accession (Same CDS sequence) XM_010821394.3 , NM_174503.1 , XM_005227485.3 , XM_005227485.4 , XM_010821394.2 Accession Version XM_010821394.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 234bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1525536000000 Organism Bos taurus(cattle) Product apelin isoform X1 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010821394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181ATGAATCTGC GGCGCTGCGT GCAGGCGCTC CTGCTGCTCT GGCTCTGCCT GAGCGCGGTG
TGCGGAGGAC CCCTGCTGCA GACTTCTGAC GGGAAGGAGA TGGAAGAAGG CACCATCCGA
TACCTGGTGC AGCCCAGGGG GCCGAGGAGC GGCCCAGGCC CCTGGCAGGG AGGTCGGAGG
AAGTTCCGGC GCCAGCGGCC ACGCCTCTCC CACAAGGGTC CCATGCCTTT CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_010819696.1 CDS 419..652 Translation
Target ORF information:
RefSeq Version XM_010821394.3 Organism Bos taurus(cattle) Definition Bos taurus apelin (APLN), transcript variant X2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_010821394.3
ORF Insert Sequence:
1
61
121
181ATGAATCTGC GGCGCTGCGT GCAGGCGCTC CTGCTGCTCT GGCTCTGCCT GAGCGCGGTG
TGCGGAGGAC CCCTGCTGCA GACTTCTGAC GGGAAGGAGA TGGAAGAAGG CACCATCCGA
TACCTGGTGC AGCCCAGGGG GCCGAGGAGC GGCCCAGGCC CCTGGCAGGG AGGTCGGAGG
AAGTTCCGGC GCCAGCGGCC ACGCCTCTCC CACAAGGGTC CCATGCCTTT CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OBa35135 Clone ID Related Accession (Same CDS sequence) XM_010821394.3 , NM_174503.1 , XM_005227485.3 , XM_005227485.4 , XM_010821394.2 Accession Version XM_005227485.3 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 234bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1453651200000 Organism Bos taurus(cattle) Product apelin isoform X1 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104655.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181ATGAATCTGC GGCGCTGCGT GCAGGCGCTC CTGCTGCTCT GGCTCTGCCT GAGCGCGGTG
TGCGGAGGAC CCCTGCTGCA GACTTCTGAC GGGAAGGAGA TGGAAGAAGG CACCATCCGA
TACCTGGTGC AGCCCAGGGG GCCGAGGAGC GGCCCAGGCC CCTGGCAGGG AGGTCGGAGG
AAGTTCCGGC GCCAGCGGCC ACGCCTCTCC CACAAGGGTC CCATGCCTTT CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_005227542.1 CDS 335..568 Translation
Target ORF information:
RefSeq Version XM_005227485.3 Organism Bos taurus(cattle) Definition Bos taurus apelin (APLN), transcript variant X2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_005227485.3
ORF Insert Sequence:
1
61
121
181ATGAATCTGC GGCGCTGCGT GCAGGCGCTC CTGCTGCTCT GGCTCTGCCT GAGCGCGGTG
TGCGGAGGAC CCCTGCTGCA GACTTCTGAC GGGAAGGAGA TGGAAGAAGG CACCATCCGA
TACCTGGTGC AGCCCAGGGG GCCGAGGAGC GGCCCAGGCC CCTGGCAGGG AGGTCGGAGG
AAGTTCCGGC GCCAGCGGCC ACGCCTCTCC CACAAGGGTC CCATGCCTTT CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OBa35135 Clone ID Related Accession (Same CDS sequence) XM_010821394.3 , NM_174503.1 , XM_005227485.3 , XM_005227485.4 , XM_010821394.2 Accession Version XM_005227485.4 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 234bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1525536000000 Organism Bos taurus(cattle) Product apelin isoform X1 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227485.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181ATGAATCTGC GGCGCTGCGT GCAGGCGCTC CTGCTGCTCT GGCTCTGCCT GAGCGCGGTG
TGCGGAGGAC CCCTGCTGCA GACTTCTGAC GGGAAGGAGA TGGAAGAAGG CACCATCCGA
TACCTGGTGC AGCCCAGGGG GCCGAGGAGC GGCCCAGGCC CCTGGCAGGG AGGTCGGAGG
AAGTTCCGGC GCCAGCGGCC ACGCCTCTCC CACAAGGGTC CCATGCCTTT CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_005227542.1 CDS 373..606 Translation
Target ORF information:
RefSeq Version XM_005227485.4 Organism Bos taurus(cattle) Definition Bos taurus apelin (APLN), transcript variant X1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_005227485.4
ORF Insert Sequence:
1
61
121
181ATGAATCTGC GGCGCTGCGT GCAGGCGCTC CTGCTGCTCT GGCTCTGCCT GAGCGCGGTG
TGCGGAGGAC CCCTGCTGCA GACTTCTGAC GGGAAGGAGA TGGAAGAAGG CACCATCCGA
TACCTGGTGC AGCCCAGGGG GCCGAGGAGC GGCCCAGGCC CCTGGCAGGG AGGTCGGAGG
AAGTTCCGGC GCCAGCGGCC ACGCCTCTCC CACAAGGGTC CCATGCCTTT CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OBa35135 Clone ID Related Accession (Same CDS sequence) XM_010821394.3 , NM_174503.1 , XM_005227485.3 , XM_005227485.4 , XM_010821394.2 Accession Version XM_010821394.2 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 234bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1453651200000 Organism Bos taurus(cattle) Product apelin isoform X1 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104655.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181ATGAATCTGC GGCGCTGCGT GCAGGCGCTC CTGCTGCTCT GGCTCTGCCT GAGCGCGGTG
TGCGGAGGAC CCCTGCTGCA GACTTCTGAC GGGAAGGAGA TGGAAGAAGG CACCATCCGA
TACCTGGTGC AGCCCAGGGG GCCGAGGAGC GGCCCAGGCC CCTGGCAGGG AGGTCGGAGG
AAGTTCCGGC GCCAGCGGCC ACGCCTCTCC CACAAGGGTC CCATGCCTTT CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_010819696.1 CDS 79..312 Translation
Target ORF information:
RefSeq Version XM_010821394.2 Organism Bos taurus(cattle) Definition Bos taurus apelin (APLN), transcript variant X1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_010821394.2
ORF Insert Sequence:
1
61
121
181ATGAATCTGC GGCGCTGCGT GCAGGCGCTC CTGCTGCTCT GGCTCTGCCT GAGCGCGGTG
TGCGGAGGAC CCCTGCTGCA GACTTCTGAC GGGAAGGAGA TGGAAGAAGG CACCATCCGA
TACCTGGTGC AGCCCAGGGG GCCGAGGAGC GGCCCAGGCC CCTGGCAGGG AGGTCGGAGG
AAGTTCCGGC GCCAGCGGCC ACGCCTCTCC CACAAGGGTC CCATGCCTTT CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
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PubMed
Apelin and APJ receptor expression in granulosa and theca cells during different stages of follicular development in the bovine ovary: Involvement of apoptosis and hormonal regulation.
Animal reproduction science116(1-2)28-37(2009 Nov)
Shimizu T,Kosaka N,Murayama C,Tetsuka M,Miyamoto A
The expression of apelin and its receptor APJ during different physiological stages in the bovine ovary.
International journal of biological sciences5(4)344-50(2009 May)
Schilffarth S,Antoni B,Schams D,Meyer HH,Berisha B
A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL
Apelin, the natural ligand of the orphan receptor APJ, is abundantly secreted in the colostrum.
Biochimica et biophysica acta1452(1)25-35(1999 Oct)
Habata Y,Fujii R,Hosoya M,Fukusumi S,Kawamata Y,Hinuma S,Kitada C,Nishizawa N,Murosaki S,Kurokawa T,Onda H,Tatemoto K,Fujino M
Isolation and characterization of a novel endogenous peptide ligand for the human APJ receptor.
Biochemical and biophysical research communications251(2)471-6(1998 Oct)
Tatemoto K,Hosoya M,Habata Y,Fujii R,Kakegawa T,Zou MX,Kawamata Y,Fukusumi S,Hinuma S,Kitada C,Kurokawa T,Onda H,Fujino M
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