INSL3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	INSL3
Entrez Gene ID	281870
Full Name	insulin like 3
Synonyms	RLF
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174365.2	NP_776790.1	insulin-like 3 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	INSL3	NP_005534.2
Mus musculus (house mouse)	Insl3	NP_038592.3
Rattus norvegicus (Norway rat)	Insl3	NP_446132.1
Pan troglodytes (chimpanzee)	INSL3	NP_001171286.1
Canis lupus familiaris (dog)	INSL3	NP_001002962.1
Bos taurus (cattle)	INSL3	NP_776790.1

Gene Ontology
Bibliography

Related articles in PubMed

Relationships of plasma insulin-like peptide 3, testosterone, inhibin, and insulin-like growth factor-I concentrations with scrotal circumference and testicular weight in Japanese Black beef bull calves.
Sakase M, Kitagawa K, Kibushi M, Kawate N, Weerakoon WWPN, Hannan MA, Kohama N, Tamada H
The Journal of reproduction and development64(5)401-407(2018 Oct)

Plasma IGF-I, INSL3, testosterone, inhibin concentrations and scrotal circumferences surrounding puberty in Japanese Black beef bulls with normal and abnormal semen.
Weerakoon WWPN, Sakase M, Kawate N, Hannan MA, Kohama N, Tamada H
Theriogenology11454-62(2018 Jul)

Plasma insulin-like peptide 3 concentrations are acutely regulated by luteinizing hormone in pubertal Japanese Black beef bulls.
Hannan MA, Fukami Y, Kawate N, Sakase M, Fukushima M, Pathirana IN, Büllesbach EE, Inaba T, Tamada H
Theriogenology84(9)1530-5(2015 Dec)

Ovarian expression of insulin-like peptide 3 (INSL3) and its receptor (RXFP2) during development of bovine antral follicles and corpora lutea and measurement of circulating INSL3 levels during synchronized estrous cycles.
Satchell L, Glister C, Bleach EC, Glencross RG, Bicknell AB, Dai Y, Anand-Ivell R, Ivell R, Knight PG
Endocrinology154(5)1897-906(2013 May)

Functional link between bone morphogenetic proteins and insulin-like peptide 3 signaling in modulating ovarian androgen production.
Glister C, Satchell L, Bathgate RA, Wade JD, Dai Y, Ivell R, Anand-Ivell R, Rodgers RJ, Knight PG
Proceedings of the National Academy of Sciences of the United States of America110(15)E1426-35(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following INSL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the INSL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41241	NM_174365.2 Latest version!	Bos taurus insulin like 3 (INSL3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

INSL3 ( NM_174365.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776790.1 Bos taurus insulin like 3 (INSL3), mRNA.

Price & Availability↑

CloneID	OBa41241
Clone ID Related Accession (Same CDS sequence)	NM_174365.2
Accession Version	NM_174365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Bos taurus(cattle)
Product	insulin-like 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF094580.1. On Jun 3, 2003 this sequence version replaced NM_174365.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF094580.1, DT860268.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145429 [ECO:0000348] ##Evidence-Data-END##

ATGGACCGTC GTCCGCTCAC CTGGGCTCTG GTGCTGCTGG GCCCGGCCCT TGCAATCGCC 
CTCGGTCCTG CAGCCGCGCA GGAGGCGCCT GAGAAACTGT GTGGCCACCA CTTCGTGCGC 
GCGCTCGTGC GGCTGTGCGG CGGACCGCGC TGGTCTTCCG AGGAGGACGG GCGACCTGTG 
GCTGGCGGCG ACCGTGAGCT CCTACGGTGG CTGGAAGGAC AACATCTCCT CCATGGGCTG 
ATGGCCAGTG GGGACCCCGT GCTGGTACTG GCCCCACAGC CCCTGCCCCA GGCTTCTCGC 
CATCACCACC ACCGCCGAGC AACTGCCATC AACCCTGCCC GCCACTGCTG CCTCAGCGGC 
TGCACCCGGC AAGACCTGCT GACCCTCTGT CCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776790.1
CDS	33..431
Translation	MDRRPLTWALVLLGPALAIALGPAAAQEAPEKLCGHHFVRALVRLCGGPRWSSEEDGRPVAGGDRELLRWLEGQHLLHGLMASGDPVLVLAPQPLPQASRHHHHRRATAINPARHCCLSGCTRQDLLTLCPH

Target ORF information:

RefSeq Version	NM_174365.2
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin like 3 (INSL3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174365.2

ORF Insert Sequence:

ATGGACCGTC GTCCGCTCAC CTGGGCTCTG GTGCTGCTGG GCCCGGCCCT TGCAATCGCC 
CTCGGTCCTG CAGCCGCGCA GGAGGCGCCT GAGAAACTGT GTGGCCACCA CTTCGTGCGC 
GCGCTCGTGC GGCTGTGCGG CGGACCGCGC TGGTCTTCCG AGGAGGACGG GCGACCTGTG 
GCTGGCGGCG ACCGTGAGCT CCTACGGTGG CTGGAAGGAC AACATCTCCT CCATGGGCTG 
ATGGCCAGTG GGGACCCCGT GCTGGTACTG GCCCCACAGC CCCTGCCCCA GGCTTCTCGC 
CATCACCACC ACCGCCGAGC AACTGCCATC AACCCTGCCC GCCACTGCTG CCTCAGCGGC 
TGCACCCGGC AAGACCTGCT GACCCTCTGT CCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Relationships of plasma insulin-like peptide 3, testosterone, inhibin, and insulin-like growth factor-I concentrations with scrotal circumference and testicular weight in Japanese Black beef bull calves.
The Journal of reproduction and development64(5)401-407(2018 Oct)
Sakase M,Kitagawa K,Kibushi M,Kawate N,Weerakoon WWPN,Hannan MA,Kohama N,Tamada H

Plasma IGF-I, INSL3, testosterone, inhibin concentrations and scrotal circumferences surrounding puberty in Japanese Black beef bulls with normal and abnormal semen.
Theriogenology11454-62(2018 Jul)
Weerakoon WWPN,Sakase M,Kawate N,Hannan MA,Kohama N,Tamada H

Plasma insulin-like peptide 3 concentrations are acutely regulated by luteinizing hormone in pubertal Japanese Black beef bulls.
Theriogenology84(9)1530-5(2015 Dec)
Hannan MA,Fukami Y,Kawate N,Sakase M,Fukushima M,Pathirana IN,Büllesbach EE,Inaba T,Tamada H

Ovarian expression of insulin-like peptide 3 (INSL3) and its receptor (RXFP2) during development of bovine antral follicles and corpora lutea and measurement of circulating INSL3 levels during synchronized estrous cycles.
Endocrinology154(5)1897-906(2013 May)
Satchell L,Glister C,Bleach EC,Glencross RG,Bicknell AB,Dai Y,Anand-Ivell R,Ivell R,Knight PG

Functional link between bone morphogenetic proteins and insulin-like peptide 3 signaling in modulating ovarian androgen production.
Proceedings of the National Academy of Sciences of the United States of America110(15)E1426-35(2013 Apr)
Glister C,Satchell L,Bathgate RA,Wade JD,Dai Y,Ivell R,Anand-Ivell R,Rodgers RJ,Knight PG

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INSL3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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