GRK1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRK1
Entrez Gene ID	281457
Full Name	G protein-coupled receptor kinase 1
Synonyms	RHOK
General protein information	Preferred Names G protein-coupled receptor kinase 1 Names rhodopsin kinase RK
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174173.2	NP_776598.1	rhodopsin kinase GRK1
XM_010810973.2	XP_010809275.1	rhodopsin kinase isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	GRK1	XP_001105738.1
Rattus norvegicus (Norway rat)	Grk1	NP_112358.1
Danio rerio (zebrafish)	grk1a	NP_001029353.2
Pan troglodytes (chimpanzee)	GRK1	XP_003314275.1
Mus musculus (house mouse)	Grk1	NP_036011.3
Homo sapiens (human)	GRK1	NP_002920.1
Canis lupus familiaris (dog)	GRK1	XP_003639862.1
Xenopus tropicalis (tropical clawed frog)	grk1	XP_002937018.2
Bos taurus (cattle)	GRK1	NP_776598.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulatory function of the C-terminal segment of guanylate cyclase-activating protein 2.
Zernii EY, Grigoriev II, Nazipova AA, Scholten A, Kolpakova TV, Zinchenko DV, Kazakov AS, Senin II, Permyakov SE, Dell'Orco D, Philippov PP, Koch KW
Biochimica et biophysica acta1854(10 Pt A)1325-37(2015 Oct)

Regulation of the methylation status of G protein-coupled receptor kinase 1 (rhodopsin kinase).
Kutuzov MA, Andreeva AV, Bennett N
Cellular signalling24(12)2259-67(2012 Dec)

Structure of a monomeric variant of rhodopsin kinase at 2.5 Å resolution.
Tesmer JJ, Nance MR, Singh P, Lee H
Acta crystallographica. Section F, Structural biology and crystallization communications68(Pt 6)622-5(2012 Jun)

Involvement of the recoverin C-terminal segment in recognition of the target enzyme rhodopsin kinase.
Zernii EY, Komolov KE, Permyakov SE, Kolpakova T, Dell'orco D, Poetzsch A, Knyazeva EL, Grigoriev II, Permyakov EA, Senin II, Philippov PP, Koch KW
The Biochemical journal435(2)441-50(2011 Apr)

Monomeric rhodopsin is sufficient for normal rhodopsin kinase (GRK1) phosphorylation and arrestin-1 binding.
Bayburt TH, Vishnivetskiy SA, McLean MA, Morizumi T, Huang CC, Tesmer JJ, Ernst OP, Sligar SG, Gurevich VV
The Journal of biological chemistry286(2)1420-8(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GRK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42648	NM_174173.2 Latest version!	Bos taurus G protein-coupled receptor kinase 1 (GRK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa45143	XM_010810973.2 Latest version!	Bos taurus G protein-coupled receptor kinase 1 (GRK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRK1 ( NM_174173.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776598.1 Bos taurus G protein-coupled receptor kinase 1 (GRK1), mRNA.

Price & Availability↑

CloneID	OBa42648
Clone ID Related Accession (Same CDS sequence)	NM_174173.2
Accession Version	NM_174173.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Bos taurus(cattle)
Product	rhodopsin kinase GRK1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M73836.1. On or before Oct 4, 2005 this sequence version replaced XM_870643.1, NM_174173.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M73836.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145474 [ECO:0000348] ##Evidence-Data-END##

ATGGATTTCG GGTCCCTGGA GACGGTGGTG GCCAATTCAG CCTTCATTGC GGCCCGGGGC 
AGCTTCGATG CGAGCAGCGG CCCGGCCTCT CGGGACAGGA AGTACCTGGC CAGGCTCAAG 
CTGCCCCCGC TGTCCAAGTG CGAGGCGCTC CGGGAGAGCC TGGACCTGGG CTTCGAGGGC 
ATGTGCCTGG AGCAGCCCAT TGGCAAGCGG CTCTTCCAGC AGTTCCTGCG GACCCACGAG 
CAGCACGGGC CAGCCCTGCA GCTCTGGAAG GACATCGAGG ACTACGACAC GGCCGACGAT 
GCCCTCCGGC CGCAGAAGGC CCAGGCCCTC CGGGCCGCGT ACCTGGAGCC CCAGGCCCAG 
CTCTTCTGCA GCTTCCTGGA TGCGGAGACG GTGGCACGGG CCCGCGCGGG GGCGGGGGAC 
GGGCTCTTCC AGCCCCTGCT GCGGGCCGTC CTGGCGCACC TCGGCCAGGC CCCCTTCCAG 
GAGTTCCTGG ACAGCCTCTA CTTCCTGCGC TTCTTGCAGT GGAAGTGGCT GGAGGCCCAG 
CCCATGGGCG AGGACTGGTT CCTAGACTTC AGGGTGCTGG GGCGGGGCGG CTTCGGGGAG 
GTGTTCGCCT GCCAGATGAA GGCCACCGGC AAGCTGTATG CGTGCAAGAA GCTGAACAAG 
AAGCGGCTGA AGAAGCGGAA GGGGTACCAG GGCGCGATGG TGGAGAAGAA GATCCTCGCC 
AAAGTGCACA GCAGGTTCAT TGTGTCTCTG GCCTACGCCT TCGAGACCAA GACGGACCTC 
TGCCTAGTGA TGACCATCAT GAACGGAGGT GACATTCGGT ACCACATCTA CAACGTGGAC 
GAGGACAACC CCGGCTTCCA GGAGCCTCGT GCCATCTTCT ACACGGCCCA GATCGTCAGC 
GGCCTGGAGC ACCTGCACCA GCGGAACATC ATCTACCGAG ACCTCAAGCC CGAGAACGTG 
CTGCTGGACG ACGATGGCAA CGTCCGCATT TCCGACCTTG GGCTGGCCGT GGAGCTGAAG 
GCAGGGCAGA CCAAGACCAA GGGCTATGCG GGGACCCCAG GGTTCATGGC CCCTGAGCTC 
CTGCTGGGGG AGGAGTACGA CTTTTCCGTA GATTACTTCG CGCTGGGTGT CACGCTGTAC 
GAGATGATCG CGGCCAGAGG GCCCTTTCGA GCCCGTGGAG AGAAGGTAGA GAACAAGGAA 
CTGAAGCAGA GGGTCCTGGA GCAGGCGGTC ACCTACCCCG ACAAGTTCAG CCCGGCCAGC 
AAAGACTTCT GCGAGGCACT GCTGCAGAAG GACCCTGAGA AGCGCCTGGG GTTCCGGGAC 
GGCTCCTGTG ATGGGCTCCG CACGCACCCC CTCTTCAGAG ACATCAGCTG GCGGCAGCTG 
GAGGCTGGGA TGCTGACCCC GCCCTTCGTC CCAGACTCCA GGACCGTCTA TGCAAAGAAT 
ATCCAGGATG TGGGTGCCTT CTCCACGGTC AAGGGCGTGG CCTTTGAAAA AGCGGACACG 
GAATTCTTCC AGGAGTTCGC CTCGGGCACC TGCCCCATCC CCTGGCAGGA GGAGATGATC 
GAGACGGGCG TCTTTGGGGA CTTGAACGTG TGGCGGCCCG ACGGGCAGAT GCCGGATGAC 
ATGAAGGGCG TCTCTGGGCA GGAGGCAGCC CCCTCATCCA AGTCGGGCAT GTGTGTGCTT 
TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776598.1
CDS	205..1890
Translation	MDFGSLETVVANSAFIAARGSFDASSGPASRDRKYLARLKLPPLSKCEALRESLDLGFEGMCLEQPIGKRLFQQFLRTHEQHGPALQLWKDIEDYDTADDALRPQKAQALRAAYLEPQAQLFCSFLDAETVARARAGAGDGLFQPLLRAVLAHLGQAPFQEFLDSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGRGGFGEVFACQMKATGKLYACKKLNKKRLKKRKGYQGAMVEKKILAKVHSRFIVSLAYAFETKTDLCLVMTIMNGGDIRYHIYNVDEDNPGFQEPRAIFYTAQIVSGLEHLHQRNIIYRDLKPENVLLDDDGNVRISDLGLAVELKAGQTKTKGYAGTPGFMAPELLLGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKQRVLEQAVTYPDKFSPASKDFCEALLQKDPEKRLGFRDGSCDGLRTHPLFRDISWRQLEAGMLTPPFVPDSRTVYAKNIQDVGAFSTVKGVAFEKADTEFFQEFASGTCPIPWQEEMIETGVFGDLNVWRPDGQMPDDMKGVSGQEAAPSSKSGMCVLS

Target ORF information:

RefSeq Version	NM_174173.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 1 (GRK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174173.2

ORF Insert Sequence:

ATGGATTTCG GGTCCCTGGA GACGGTGGTG GCCAATTCAG CCTTCATTGC GGCCCGGGGC 
AGCTTCGATG CGAGCAGCGG CCCGGCCTCT CGGGACAGGA AGTACCTGGC CAGGCTCAAG 
CTGCCCCCGC TGTCCAAGTG CGAGGCGCTC CGGGAGAGCC TGGACCTGGG CTTCGAGGGC 
ATGTGCCTGG AGCAGCCCAT TGGCAAGCGG CTCTTCCAGC AGTTCCTGCG GACCCACGAG 
CAGCACGGGC CAGCCCTGCA GCTCTGGAAG GACATCGAGG ACTACGACAC GGCCGACGAT 
GCCCTCCGGC CGCAGAAGGC CCAGGCCCTC CGGGCCGCGT ACCTGGAGCC CCAGGCCCAG 
CTCTTCTGCA GCTTCCTGGA TGCGGAGACG GTGGCACGGG CCCGCGCGGG GGCGGGGGAC 
GGGCTCTTCC AGCCCCTGCT GCGGGCCGTC CTGGCGCACC TCGGCCAGGC CCCCTTCCAG 
GAGTTCCTGG ACAGCCTCTA CTTCCTGCGC TTCTTGCAGT GGAAGTGGCT GGAGGCCCAG 
CCCATGGGCG AGGACTGGTT CCTAGACTTC AGGGTGCTGG GGCGGGGCGG CTTCGGGGAG 
GTGTTCGCCT GCCAGATGAA GGCCACCGGC AAGCTGTATG CGTGCAAGAA GCTGAACAAG 
AAGCGGCTGA AGAAGCGGAA GGGGTACCAG GGCGCGATGG TGGAGAAGAA GATCCTCGCC 
AAAGTGCACA GCAGGTTCAT TGTGTCTCTG GCCTACGCCT TCGAGACCAA GACGGACCTC 
TGCCTAGTGA TGACCATCAT GAACGGAGGT GACATTCGGT ACCACATCTA CAACGTGGAC 
GAGGACAACC CCGGCTTCCA GGAGCCTCGT GCCATCTTCT ACACGGCCCA GATCGTCAGC 
GGCCTGGAGC ACCTGCACCA GCGGAACATC ATCTACCGAG ACCTCAAGCC CGAGAACGTG 
CTGCTGGACG ACGATGGCAA CGTCCGCATT TCCGACCTTG GGCTGGCCGT GGAGCTGAAG 
GCAGGGCAGA CCAAGACCAA GGGCTATGCG GGGACCCCAG GGTTCATGGC CCCTGAGCTC 
CTGCTGGGGG AGGAGTACGA CTTTTCCGTA GATTACTTCG CGCTGGGTGT CACGCTGTAC 
GAGATGATCG CGGCCAGAGG GCCCTTTCGA GCCCGTGGAG AGAAGGTAGA GAACAAGGAA 
CTGAAGCAGA GGGTCCTGGA GCAGGCGGTC ACCTACCCCG ACAAGTTCAG CCCGGCCAGC 
AAAGACTTCT GCGAGGCACT GCTGCAGAAG GACCCTGAGA AGCGCCTGGG GTTCCGGGAC 
GGCTCCTGTG ATGGGCTCCG CACGCACCCC CTCTTCAGAG ACATCAGCTG GCGGCAGCTG 
GAGGCTGGGA TGCTGACCCC GCCCTTCGTC CCAGACTCCA GGACCGTCTA TGCAAAGAAT 
ATCCAGGATG TGGGTGCCTT CTCCACGGTC AAGGGCGTGG CCTTTGAAAA AGCGGACACG 
GAATTCTTCC AGGAGTTCGC CTCGGGCACC TGCCCCATCC CCTGGCAGGA GGAGATGATC 
GAGACGGGCG TCTTTGGGGA CTTGAACGTG TGGCGGCCCG ACGGGCAGAT GCCGGATGAC 
ATGAAGGGCG TCTCTGGGCA GGAGGCAGCC CCCTCATCCA AGTCGGGCAT GTGTGTGCTT 
TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK1 ( XM_010810973.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809275.1 Bos taurus G protein-coupled receptor kinase 1 (GRK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45143
Clone ID Related Accession (Same CDS sequence)	XM_010810973.2
Accession Version	XM_010810973.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	rhodopsin kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810973.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTCG GGTCCCTGGA GACGGTGGTG GCCAATTCAG CCTTCATTGC GGCCCGGGGC 
AGCTTCGATG CGAGCAGCGG CCCGGCCTCT CGGGACAGGA AGTACCTGGC CAGGCTCAAG 
CTGCCCCCGC TGTCCAAGTG CGAGGCGCTC CGGGAGAGCC TGGACCTGGG CTTCGAGGGC 
ATGTGCCTGG AGCAGCCCAT TGGCAAGCGG CTCTTCCAGC AGTTCCTGCG GACCCACGAG 
CAGCACGGGC CAGCCCTGCA GCTCTGGAAG GACATCGAGG ACTACGACAC GGCCGACGAT 
GCCCTCCGGC CGCAGAAGGC CCAGGCCCTC CGGGCCGCGT ACCTGGAGCC CCAGGCCCAG 
CTCTTCTGCA GCTTCCTGGA TGCGGAGACG GTGGCACGGG CCCGCGCGGG GGCGGGGGAC 
GGGCTCTTCC AGCCCCTGCT GCGGGCCGTC CTGGCGCACC TCGGCCAGGC CCCCTTCCAG 
GAGTTCCTGG ACAGCCTCTA CTTCCTGCGC TTCTTGCAGT GGAAGTGGCT GGAGGCCCAG 
CCCATGGGCG AGGACTGGTT CCTAGACTTC AGGGTGCTGG GGCGGGGCGG CTTCGGGGAG 
GTGTTCGCCT GCCAGATGAA GGCCACCGGC AAGCTGTATG CGTGCAAGAA GCTGAACAAG 
AAGCGGCTGA AGAAGCGGAA GGGGTACCAG GGCGCGATGG TGGAGAAGAA GATCCTCGCC 
AAAGTGCACA GCAGGTTCAT TGTGTCTCTG GCCTACGCCT TCGAGACCAA GACGGACCTC 
TGCCTAGTGA TGACCATCAT GAACGGAGGT GACATTCGGT ACCACATCTA CAACGTGGAC 
GAGGACAACC CCGGCTTCCA GGAGCCTCGT GCCATCTTCT ACACGGCCCA GATCGTCAGC 
GGCCTGGAGC ACCTGCACCA GCGGAACATC ATCTACCGAG ACCTCAAGCC CGAGAACGTG 
CTGCTGGACG ACGATGGCAA CGTCCGCATT TCCGACCTTG GGCTGGCCGT GGAGCTGAAG 
GCAGGGCAGA CCAAGACCAA GGGCTATGCG GGGACCCCAG GGTTCATGGC CCCTGAGCTC 
CTGCTGGGGG AGGAGTACGA CTTTTCCGTA GATTACTTCG CGCTGGGTGT CACGCTGTAC 
GAGATGATCG CGGCCAGAGG GCCCTTTCGA GCCCGTGGAG AGAAGGTAGA GAACAAGGAA 
CTGAAGCAGA GGGTCCTGGA GCAGGCGGTC ACCTACCCCG ACAAGTTCAG CCCGGCCAGC 
AAAGACTTCT GCGAGGCACT GCTGCAGAAG GACCCTGAGA AGCGCCTGGG GTTCCGGGAC 
GGCTCCTGTG ATGGGCTCCG CACGCACCCC CTCTTCAGAG ACATCAGCTG GCGGCAGCTG 
GAGGCTGGAA CCCACGAGCC AAGTCAAGCA GGGTTTCTCC AGTCGTCATC CAACCCGAGT 
TTGCTGCCGG GTCTTTGGGA TGCTGACCCC GCCCTTCGTC CCAGACTCCA GGACCGTCTA 
TGCAAAGAAT ATCCAGGATG TGGGTGCCTT CTCCACGGTC AAGGGCGTGG CCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809275.1
CDS	205..1761
Translation	MDFGSLETVVANSAFIAARGSFDASSGPASRDRKYLARLKLPPLSKCEALRESLDLGFEGMCLEQPIGKRLFQQFLRTHEQHGPALQLWKDIEDYDTADDALRPQKAQALRAAYLEPQAQLFCSFLDAETVARARAGAGDGLFQPLLRAVLAHLGQAPFQEFLDSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGRGGFGEVFACQMKATGKLYACKKLNKKRLKKRKGYQGAMVEKKILAKVHSRFIVSLAYAFETKTDLCLVMTIMNGGDIRYHIYNVDEDNPGFQEPRAIFYTAQIVSGLEHLHQRNIIYRDLKPENVLLDDDGNVRISDLGLAVELKAGQTKTKGYAGTPGFMAPELLLGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKQRVLEQAVTYPDKFSPASKDFCEALLQKDPEKRLGFRDGSCDGLRTHPLFRDISWRQLEAGTHEPSQAGFLQSSSNPSLLPGLWDADPALRPRLQDRLCKEYPGCGCLLHGQGRGL

Target ORF information:

RefSeq Version	XM_010810973.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 1 (GRK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810973.2

ORF Insert Sequence:

ATGGATTTCG GGTCCCTGGA GACGGTGGTG GCCAATTCAG CCTTCATTGC GGCCCGGGGC 
AGCTTCGATG CGAGCAGCGG CCCGGCCTCT CGGGACAGGA AGTACCTGGC CAGGCTCAAG 
CTGCCCCCGC TGTCCAAGTG CGAGGCGCTC CGGGAGAGCC TGGACCTGGG CTTCGAGGGC 
ATGTGCCTGG AGCAGCCCAT TGGCAAGCGG CTCTTCCAGC AGTTCCTGCG GACCCACGAG 
CAGCACGGGC CAGCCCTGCA GCTCTGGAAG GACATCGAGG ACTACGACAC GGCCGACGAT 
GCCCTCCGGC CGCAGAAGGC CCAGGCCCTC CGGGCCGCGT ACCTGGAGCC CCAGGCCCAG 
CTCTTCTGCA GCTTCCTGGA TGCGGAGACG GTGGCACGGG CCCGCGCGGG GGCGGGGGAC 
GGGCTCTTCC AGCCCCTGCT GCGGGCCGTC CTGGCGCACC TCGGCCAGGC CCCCTTCCAG 
GAGTTCCTGG ACAGCCTCTA CTTCCTGCGC TTCTTGCAGT GGAAGTGGCT GGAGGCCCAG 
CCCATGGGCG AGGACTGGTT CCTAGACTTC AGGGTGCTGG GGCGGGGCGG CTTCGGGGAG 
GTGTTCGCCT GCCAGATGAA GGCCACCGGC AAGCTGTATG CGTGCAAGAA GCTGAACAAG 
AAGCGGCTGA AGAAGCGGAA GGGGTACCAG GGCGCGATGG TGGAGAAGAA GATCCTCGCC 
AAAGTGCACA GCAGGTTCAT TGTGTCTCTG GCCTACGCCT TCGAGACCAA GACGGACCTC 
TGCCTAGTGA TGACCATCAT GAACGGAGGT GACATTCGGT ACCACATCTA CAACGTGGAC 
GAGGACAACC CCGGCTTCCA GGAGCCTCGT GCCATCTTCT ACACGGCCCA GATCGTCAGC 
GGCCTGGAGC ACCTGCACCA GCGGAACATC ATCTACCGAG ACCTCAAGCC CGAGAACGTG 
CTGCTGGACG ACGATGGCAA CGTCCGCATT TCCGACCTTG GGCTGGCCGT GGAGCTGAAG 
GCAGGGCAGA CCAAGACCAA GGGCTATGCG GGGACCCCAG GGTTCATGGC CCCTGAGCTC 
CTGCTGGGGG AGGAGTACGA CTTTTCCGTA GATTACTTCG CGCTGGGTGT CACGCTGTAC 
GAGATGATCG CGGCCAGAGG GCCCTTTCGA GCCCGTGGAG AGAAGGTAGA GAACAAGGAA 
CTGAAGCAGA GGGTCCTGGA GCAGGCGGTC ACCTACCCCG ACAAGTTCAG CCCGGCCAGC 
AAAGACTTCT GCGAGGCACT GCTGCAGAAG GACCCTGAGA AGCGCCTGGG GTTCCGGGAC 
GGCTCCTGTG ATGGGCTCCG CACGCACCCC CTCTTCAGAG ACATCAGCTG GCGGCAGCTG 
GAGGCTGGAA CCCACGAGCC AAGTCAAGCA GGGTTTCTCC AGTCGTCATC CAACCCGAGT 
TTGCTGCCGG GTCTTTGGGA TGCTGACCCC GCCCTTCGTC CCAGACTCCA GGACCGTCTA 
TGCAAAGAAT ATCCAGGATG TGGGTGCCTT CTCCACGGTC AAGGGCGTGG CCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulatory function of the C-terminal segment of guanylate cyclase-activating protein 2.
Biochimica et biophysica acta1854(10 Pt A)1325-37(2015 Oct)
Zernii EY,Grigoriev II,Nazipova AA,Scholten A,Kolpakova TV,Zinchenko DV,Kazakov AS,Senin II,Permyakov SE,Dell'Orco D,Philippov PP,Koch KW

Regulation of the methylation status of G protein-coupled receptor kinase 1 (rhodopsin kinase).
Cellular signalling24(12)2259-67(2012 Dec)
Kutuzov MA,Andreeva AV,Bennett N

Structure of a monomeric variant of rhodopsin kinase at 2.5 Å resolution.
Acta crystallographica. Section F, Structural biology and crystallization communications68(Pt 6)622-5(2012 Jun)
Tesmer JJ,Nance MR,Singh P,Lee H

Involvement of the recoverin C-terminal segment in recognition of the target enzyme rhodopsin kinase.
The Biochemical journal435(2)441-50(2011 Apr)
Zernii EY,Komolov KE,Permyakov SE,Kolpakova T,Dell'orco D,Poetzsch A,Knyazeva EL,Grigoriev II,Permyakov EA,Senin II,Philippov PP,Koch KW

Monomeric rhodopsin is sufficient for normal rhodopsin kinase (GRK1) phosphorylation and arrestin-1 binding.
The Journal of biological chemistry286(2)1420-8(2011 Jan)
Bayburt TH,Vishnivetskiy SA,McLean MA,Morizumi T,Huang CC,Tesmer JJ,Ernst OP,Sligar SG,Gurevich VV

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GRK1 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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