TCF25 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TCF25
Entrez Gene ID	100848970
Full Name	transcription factor 25
General protein information	Preferred Names transcription factor 25 Names transcription factor 25
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099177.1	NP_001092647.1	transcription factor 25
XM_024977926.1	XP_024833694.1	transcription factor 25 isoform X1
XM_010814600.2	XP_010812902.2	transcription factor 25 isoform X1
XM_015467326.1	XP_015322812.1	transcription factor 25 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Unexpected cross-species contamination in genome sequencing projects.
Merchant S, Wood DE, Salzberg SL
PeerJ2e675(2014)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TCF25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TCF25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa02246	NM_001099177.1 Latest version!	Bos taurus transcription factor 25 (TCF25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa224774	XM_024977926.1 Latest version!	Bos taurus transcription factor 25 (TCF25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa93387	XM_010814600.2 Latest version!	Bos taurus transcription factor 25 (basic helix-loop-helix) (TCF25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa94969	XM_015467326.1 Latest version!	Bos taurus transcription factor 25 (basic helix-loop-helix) (TCF25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TCF25 ( NM_001099177.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001092647.1 Bos taurus transcription factor 25 (TCF25), mRNA.

Price & Availability↑

CloneID	OBa02246
Clone ID Related Accession (Same CDS sequence)	NM_001099177.1
Accession Version	NM_001099177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	transcription factor 25
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC142262.1. On Aug 4, 2007 this sequence version replaced XM_870719.2. ##Evidence-Data-START## Transcript exon combination :: BC142262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGTCGCGCC GGGCCCTCCG GAGGCTGAGA GGGGAACAGC GCGGCCAGGA GCCCCTCGGG 
CCTGGCGCCC TGCAGTTTGT CCTTCATGAT GACGATGATG TGGAAGAAGA ATGCCCAAAG 
CGGGGTCCAG GTGGCCGGCG CCCCCGGGGC GCAGGGAAGG AGGGAGTCCG AGTCAACAAC 
CGGTTCGAGC TGATAAACAT CGAGGACCTG GACGAGGCAC CCGGGGTGAA TGGGGAGAGG 
ACAGACTGTC TGCTTGTGGA TGCTGTGGTG TCAGGGAACA AGAGGAGGGC CCAGAGCGGG 
CATTCAGAGT CCACCAGGGG CGGCAATGCG GCAGACACAG CGGTTCCCTC AGAACAGTCC 
AATACCTGTA GCAAACTCCG CAAGAAGAAA AGAAAACAGA AAAATAAGAA AAACGCAGGA 
GAAACCTCGC AGAATGGCCT GGAGGACATT GACCGCATCC TGGAGAGGAT CGAGGATGCC 
AGCGGTGCCA GCCGCCCGGG CCCGCCCCCC CTGAGCACGA AGAAGCATGT CCTGTATGTG 
GAGCACAGAC ACTTGAATCC AGACACAGAG CTGAAAAGAT ATTTTGGTGC CAGAGCTGTT 
CTCGGGGAGC AGAGGCCGCG GCAGAGACAG CGCATGTACC CCAAGTGTAC ATGGCTGACC 
ACCCCGAAGA GCACGTGGCC CCGGTACACC AAACCAGGTC TGTCGATGCG GCTGTTGGAA 
TCCAGGAAAG GCCGCTCGGT CTTCGCCTTT GAGCACAGCG AGGAGTACCA ACAGACGCAG 
CACAAGTTCC TGGCCGCCGT CGAGTCCATG GAGCCCAATA ACATTGTGGT CCTGCTCCAG 
TCAAGCCCCT ACCACGTGGA CTCGCTCCTG CAGCTCAGCG ACGCATGCCG CTTTCAGGAG 
GACCAGGAGA CGGCACGCGA CCTCGTGGAG CGTGCGCTGT ACAGCATGGA GTGTGCCTTC 
CACCCCTTGT TCAGCCTCAC CAGTGGGACC TGCCGGCTGG ACTACCGCAG GCCTGAGAAC 
AGGAGCTTCT ACCTGGCCCT CTACAAGCAG ATGAGCTTCC TGGAGAAGCG GGGCTGCCCA 
CGCACGGCGC TGGAGTACTG CAAGCTCATC CTCAGCCTGG AGCCAGACGA AGACCCCCTG 
TGCATGCTGC TGCTAATCGA CCACCTGGCC TTACGAGCGC GGAGCTACGA GTACTTGATC 
CGCCTGTTCG AAGAGTGGGA GGCCCATCGG AACCTGTCCC AGCTCCCAAA CTTTGCCTTC 
TCTGTGCCGC TGGCCTATTT CCTGCTGAGC CAACAGGCAG ACCTCTCCGA GCAGGAGATG 
AGCTCCGCGA GGGAGGAGGC CTGTGTCCGG ATCCAGCAGG CGCTCACCAT GTTCCCTGGA 
GTGCTCATGC CCCTGCTCGA GTACTGCAGT GTACGCCCCG ACACCACCGT GGCCACGCAC 
CACTTCTTCG GACCCGACGC CGAAATCAGC CAGCCACCCG CCCTGAGCCA GCTGGTGAGC 
CTGTACCTGG GGAGGTCACA CTTCCTCTGG AAGGAGCCGG CCACCATGAG CTGGCTGGAG 
GAGAATGTGC ACAAGGTTCT ACAGGTGGTG GACGCCGGGG ACCCTGCCGT GGAGGCGTGT 
GAGAGCAGGC GCAAGGTGCT GTACCAGCGG GCGCCCAGGA ACATCCACCG GCATGTGGTC 
CTGTCTGAGA TCAAGGAGGC TGTGGCCGCC CTGCCGCCGG ATGTGACCAC ACAGTCTGTG 
TTGGGCTTCG ACCCTCTGCC CCCACTGGAC ACCATCTACT CCTACGTCAG ACCAGAGAGG 
CTCAGCCCCA TCAGCCACGG AAACACCATC GCCCTCTTCT TCCGCTCACT CCTGCCCAAC 
TACACTGTGG AGGGGGAGCG ACCGGAGGAC GGGGGGCCCG GGGGTCTGCA CCGTGACCAG 
GGCCTGAACA GACTGATGCT GGCTGTGCGG GACATGATGG CCAACTTCCA CTTCCACGAC 
CTGGAGGTGC TGCCCGATGA GCACCCTGAG GGCGACGGGG AGTGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092647.1
CDS	92..2122
Translation	MSRRALRRLRGEQRGQEPLGPGALQFVLHDDDDVEEECPKRGPGGRRPRGAGKEGVRVNNRFELINIEDLDEAPGVNGERTDCLLVDAVVSGNKRRAQSGHSESTRGGNAADTAVPSEQSNTCSKLRKKKRKQKNKKNAGETSQNGLEDIDRILERIEDASGASRPGPPPLSTKKHVLYVEHRHLNPDTELKRYFGARAVLGEQRPRQRQRMYPKCTWLTTPKSTWPRYTKPGLSMRLLESRKGRSVFAFEHSEEYQQTQHKFLAAVESMEPNNIVVLLQSSPYHVDSLLQLSDACRFQEDQETARDLVERALYSMECAFHPLFSLTSGTCRLDYRRPENRSFYLALYKQMSFLEKRGCPRTALEYCKLILSLEPDEDPLCMLLLIDHLALRARSYEYLIRLFEEWEAHRNLSQLPNFAFSVPLAYFLLSQQADLSEQEMSSAREEACVRIQQALTMFPGVLMPLLEYCSVRPDTTVATHHFFGPDAEISQPPALSQLVSLYLGRSHFLWKEPATMSWLEENVHKVLQVVDAGDPAVEACESRRKVLYQRAPRNIHRHVVLSEIKEAVAALPPDVTTQSVLGFDPLPPLDTIYSYVRPERLSPISHGNTIALFFRSLLPNYTVEGERPEDGGPGGLHRDQGLNRLMLAVRDMMANFHFHDLEVLPDEHPEGDGEWD

Target ORF information:

RefSeq Version	NM_001099177.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 25 (TCF25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099177.1

ORF Insert Sequence:

ATGTCGCGCC GGGCCCTCCG GAGGCTGAGA GGGGAACAGC GCGGCCAGGA GCCCCTCGGG 
CCTGGCGCCC TGCAGTTTGT CCTTCATGAT GACGATGATG TGGAAGAAGA ATGCCCAAAG 
CGGGGTCCAG GTGGCCGGCG CCCCCGGGGC GCAGGGAAGG AGGGAGTCCG AGTCAACAAC 
CGGTTCGAGC TGATAAACAT CGAGGACCTG GACGAGGCAC CCGGGGTGAA TGGGGAGAGG 
ACAGACTGTC TGCTTGTGGA TGCTGTGGTG TCAGGGAACA AGAGGAGGGC CCAGAGCGGG 
CATTCAGAGT CCACCAGGGG CGGCAATGCG GCAGACACAG CGGTTCCCTC AGAACAGTCC 
AATACCTGTA GCAAACTCCG CAAGAAGAAA AGAAAACAGA AAAATAAGAA AAACGCAGGA 
GAAACCTCGC AGAATGGCCT GGAGGACATT GACCGCATCC TGGAGAGGAT CGAGGATGCC 
AGCGGTGCCA GCCGCCCGGG CCCGCCCCCC CTGAGCACGA AGAAGCATGT CCTGTATGTG 
GAGCACAGAC ACTTGAATCC AGACACAGAG CTGAAAAGAT ATTTTGGTGC CAGAGCTGTT 
CTCGGGGAGC AGAGGCCGCG GCAGAGACAG CGCATGTACC CCAAGTGTAC ATGGCTGACC 
ACCCCGAAGA GCACGTGGCC CCGGTACACC AAACCAGGTC TGTCGATGCG GCTGTTGGAA 
TCCAGGAAAG GCCGCTCGGT CTTCGCCTTT GAGCACAGCG AGGAGTACCA ACAGACGCAG 
CACAAGTTCC TGGCCGCCGT CGAGTCCATG GAGCCCAATA ACATTGTGGT CCTGCTCCAG 
TCAAGCCCCT ACCACGTGGA CTCGCTCCTG CAGCTCAGCG ACGCATGCCG CTTTCAGGAG 
GACCAGGAGA CGGCACGCGA CCTCGTGGAG CGTGCGCTGT ACAGCATGGA GTGTGCCTTC 
CACCCCTTGT TCAGCCTCAC CAGTGGGACC TGCCGGCTGG ACTACCGCAG GCCTGAGAAC 
AGGAGCTTCT ACCTGGCCCT CTACAAGCAG ATGAGCTTCC TGGAGAAGCG GGGCTGCCCA 
CGCACGGCGC TGGAGTACTG CAAGCTCATC CTCAGCCTGG AGCCAGACGA AGACCCCCTG 
TGCATGCTGC TGCTAATCGA CCACCTGGCC TTACGAGCGC GGAGCTACGA GTACTTGATC 
CGCCTGTTCG AAGAGTGGGA GGCCCATCGG AACCTGTCCC AGCTCCCAAA CTTTGCCTTC 
TCTGTGCCGC TGGCCTATTT CCTGCTGAGC CAACAGGCAG ACCTCTCCGA GCAGGAGATG 
AGCTCCGCGA GGGAGGAGGC CTGTGTCCGG ATCCAGCAGG CGCTCACCAT GTTCCCTGGA 
GTGCTCATGC CCCTGCTCGA GTACTGCAGT GTACGCCCCG ACACCACCGT GGCCACGCAC 
CACTTCTTCG GACCCGACGC CGAAATCAGC CAGCCACCCG CCCTGAGCCA GCTGGTGAGC 
CTGTACCTGG GGAGGTCACA CTTCCTCTGG AAGGAGCCGG CCACCATGAG CTGGCTGGAG 
GAGAATGTGC ACAAGGTTCT ACAGGTGGTG GACGCCGGGG ACCCTGCCGT GGAGGCGTGT 
GAGAGCAGGC GCAAGGTGCT GTACCAGCGG GCGCCCAGGA ACATCCACCG GCATGTGGTC 
CTGTCTGAGA TCAAGGAGGC TGTGGCCGCC CTGCCGCCGG ATGTGACCAC ACAGTCTGTG 
TTGGGCTTCG ACCCTCTGCC CCCACTGGAC ACCATCTACT CCTACGTCAG ACCAGAGAGG 
CTCAGCCCCA TCAGCCACGG AAACACCATC GCCCTCTTCT TCCGCTCACT CCTGCCCAAC 
TACACTGTGG AGGGGGAGCG ACCGGAGGAC GGGGGGCCCG GGGGTCTGCA CCGTGACCAG 
GGCCTGAACA GACTGATGCT GGCTGTGCGG GACATGATGG CCAACTTCCA CTTCCACGAC 
CTGGAGGTGC TGCCCGATGA GCACCCTGAG GGCGACGGGG AGTGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF25 ( XM_024977926.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833694.1 Bos taurus transcription factor 25 (TCF25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa224774
Clone ID Related Accession (Same CDS sequence)	XM_024977926.1
Accession Version	XM_024977926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	transcription factor 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCC GGGCCCTCCG GAGGCTGAGA GGGGAACAGC GCGGCCAGGA GCCCCTCGGG 
CCTGGCGCCC TGCAGTTTGT CCTTCATGAT GACGATGATG TGGAAGAAGA ATGCCCAAAG 
CGGGGTCCAG GTGGCCGGCG CCCCCGGGGC GCAGGGAAGG AGGGAGTCCG AGTCAACAAC 
CGGTTCGAGC TGATAAACAT CGAGGACCTG GACGAGGCAC CCGGGGTGAA TGGGGAGAGG 
ACAGACTGTC TGCTTGTGGA TGCTGTGGTG TCAGGGAACA AGAGGAGGGC CCAGAGCGGG 
CATTCAGAGT CCACCAGGGG CGGCAATGCG GCAGACACAG CGGTTCCCTC AGAACAGTCC 
AATACCTGTA GCAAACTCCG CAAGAAGAAA AGAAAACAGA AAAATAAGAA AAACGCAGGA 
GAAACCTCGA ATGGCCTGGA GGACATTGAC CGCATCCTGG AGAGGATCGA GGATGCCAGC 
GGTGCCAGCC GCCCGGGCCC GCCCCCCCTG AGCACGAAGA AGCATGTCCT GTATGTGGAG 
CACAGACACT TGAATCCAGA CACAGAGCTG AAAAGATATT TTGGTGCCAG AGCTGTTCTC 
GGGGAGCAGA GGCCGCGGCA GAGACAGCGC ATGTACCCCA AGTGTACATG GCTGACCACC 
CCGAAGAGCA CGTGGCCCCG GTACACCAAA CCAGGTCTGT CGATGCGGCT GTTGGAATCC 
AGGAAAGGCC GCTCGGTCTT CGCCTTTGAG CACAGCGAGG AGTACCAACA GACGCAGCAC 
AAGTTCCTGG CCGCCGTCGA GTCCATGGAG CCCAATAACA TTGTGGTCCT GCTCCAGTCA 
AGCCCCTACC ACGTGGACTC GCTCCTGCAG CTCAGCGACG CATGCCGCTT TCAGGAGGAC 
CAGGAGACGG CACGCGACCT CGTGGAGCGT GCGCTGTACA GCATGGAGTG TGCCTTCCAC 
CCCTTGTTCA GCCTCACCAG TGGGACCTGC CGGCTGGACT ACCGCAGGCC TGAGAACAGG 
AGCTTCTACC TGGCCCTCTA CAAGCAGATG AGCTTCCTGG AGAAGCGGGG CTGCCCACGC 
ACGGCGCTGG AGTACTGCAA GCTCATCCTC AGCCTGGAGC CAGACGAAGA CCCCCTGTGC 
ATGCTGCTGC TAATCGACCA CCTGGCCTTA CGAGCGCGGA GCTACGAGTA CTTGATCCGC 
CTGTTCGAAG AGTGGGAGGC CCATCGGAAC CTGTCCCAGC TCCCAAACTT TGCCTTCTCT 
GTGCCGCTGG CCTATTTCCT GCTGAGCCAA CAGGCAGACC TCTCCGAGCA GGAGATGAGC 
TCCGCGAGGG AGGAGGCCTG TGTCCGGATC CAGCAGGCGC TCACCATGTT CCCCGGAGTG 
CTCATGCCCC TGCTCGAGTA CTGCAGTGTA CGCCCCGACA CCACCGTGGC CACGCACCAC 
TTCTTCGGAC CCGACGCCGA AATCAGCCAG CCACCCGCCC TGAGCCAGCT GGTGAGCCTG 
TACCTGGGGA GGTCACACTT CCTCTGGAAG GAGCCGGCCA CCATGAGCTG GCTGGAGGAG 
AATGTGCACA AGGTTCTACA GGTGGTGGAC GCCGGGGACC CTGCCGTGGA GGCGTGTGAG 
AGCAGGCGCA AGGTGCTGTA CCAGCGGGCG CCCAGGAACA TCCACCGGCA TGTGGTCCTG 
TCTGAGATCA AGGAGGCTGT GGCCGCCCTG CCGCCGGATG TGACCACACA GTCTGTGTTG 
GGCTTCGACC CTCTGCCCCC ACTGGACACC ATCTACTCCT ACGTCAGACC AGAGAGGCTC 
AGCCCCATCA GCCACGGAAA CACCATCGCC CTCTTCTTCC GCTCACTCCT GCCCAACTAC 
ACTGTGGAGG GGGAGCGACC GGAGGACGGG GGGCCCGGGG GTCTGCACCG TGACCAGGGC 
CTGAACAGAC TGATGCTGGC TGTGCGGGAC ATGATGGCCA ACTTCCACTT CCACGACCTG 
GAGGTGCTGC CCGAGGAGCA CCCTGAGGGC GACGGGGAGT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833694.1
CDS	140..2167
Translation	MSRRALRRLRGEQRGQEPLGPGALQFVLHDDDDVEEECPKRGPGGRRPRGAGKEGVRVNNRFELINIEDLDEAPGVNGERTDCLLVDAVVSGNKRRAQSGHSESTRGGNAADTAVPSEQSNTCSKLRKKKRKQKNKKNAGETSNGLEDIDRILERIEDASGASRPGPPPLSTKKHVLYVEHRHLNPDTELKRYFGARAVLGEQRPRQRQRMYPKCTWLTTPKSTWPRYTKPGLSMRLLESRKGRSVFAFEHSEEYQQTQHKFLAAVESMEPNNIVVLLQSSPYHVDSLLQLSDACRFQEDQETARDLVERALYSMECAFHPLFSLTSGTCRLDYRRPENRSFYLALYKQMSFLEKRGCPRTALEYCKLILSLEPDEDPLCMLLLIDHLALRARSYEYLIRLFEEWEAHRNLSQLPNFAFSVPLAYFLLSQQADLSEQEMSSAREEACVRIQQALTMFPGVLMPLLEYCSVRPDTTVATHHFFGPDAEISQPPALSQLVSLYLGRSHFLWKEPATMSWLEENVHKVLQVVDAGDPAVEACESRRKVLYQRAPRNIHRHVVLSEIKEAVAALPPDVTTQSVLGFDPLPPLDTIYSYVRPERLSPISHGNTIALFFRSLLPNYTVEGERPEDGGPGGLHRDQGLNRLMLAVRDMMANFHFHDLEVLPEEHPEGDGEWD

Target ORF information:

RefSeq Version	XM_024977926.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 25 (TCF25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977926.1

ORF Insert Sequence:

ATGTCGCGCC GGGCCCTCCG GAGGCTGAGA GGGGAACAGC GCGGCCAGGA GCCCCTCGGG 
CCTGGCGCCC TGCAGTTTGT CCTTCATGAT GACGATGATG TGGAAGAAGA ATGCCCAAAG 
CGGGGTCCAG GTGGCCGGCG CCCCCGGGGC GCAGGGAAGG AGGGAGTCCG AGTCAACAAC 
CGGTTCGAGC TGATAAACAT CGAGGACCTG GACGAGGCAC CCGGGGTGAA TGGGGAGAGG 
ACAGACTGTC TGCTTGTGGA TGCTGTGGTG TCAGGGAACA AGAGGAGGGC CCAGAGCGGG 
CATTCAGAGT CCACCAGGGG CGGCAATGCG GCAGACACAG CGGTTCCCTC AGAACAGTCC 
AATACCTGTA GCAAACTCCG CAAGAAGAAA AGAAAACAGA AAAATAAGAA AAACGCAGGA 
GAAACCTCGA ATGGCCTGGA GGACATTGAC CGCATCCTGG AGAGGATCGA GGATGCCAGC 
GGTGCCAGCC GCCCGGGCCC GCCCCCCCTG AGCACGAAGA AGCATGTCCT GTATGTGGAG 
CACAGACACT TGAATCCAGA CACAGAGCTG AAAAGATATT TTGGTGCCAG AGCTGTTCTC 
GGGGAGCAGA GGCCGCGGCA GAGACAGCGC ATGTACCCCA AGTGTACATG GCTGACCACC 
CCGAAGAGCA CGTGGCCCCG GTACACCAAA CCAGGTCTGT CGATGCGGCT GTTGGAATCC 
AGGAAAGGCC GCTCGGTCTT CGCCTTTGAG CACAGCGAGG AGTACCAACA GACGCAGCAC 
AAGTTCCTGG CCGCCGTCGA GTCCATGGAG CCCAATAACA TTGTGGTCCT GCTCCAGTCA 
AGCCCCTACC ACGTGGACTC GCTCCTGCAG CTCAGCGACG CATGCCGCTT TCAGGAGGAC 
CAGGAGACGG CACGCGACCT CGTGGAGCGT GCGCTGTACA GCATGGAGTG TGCCTTCCAC 
CCCTTGTTCA GCCTCACCAG TGGGACCTGC CGGCTGGACT ACCGCAGGCC TGAGAACAGG 
AGCTTCTACC TGGCCCTCTA CAAGCAGATG AGCTTCCTGG AGAAGCGGGG CTGCCCACGC 
ACGGCGCTGG AGTACTGCAA GCTCATCCTC AGCCTGGAGC CAGACGAAGA CCCCCTGTGC 
ATGCTGCTGC TAATCGACCA CCTGGCCTTA CGAGCGCGGA GCTACGAGTA CTTGATCCGC 
CTGTTCGAAG AGTGGGAGGC CCATCGGAAC CTGTCCCAGC TCCCAAACTT TGCCTTCTCT 
GTGCCGCTGG CCTATTTCCT GCTGAGCCAA CAGGCAGACC TCTCCGAGCA GGAGATGAGC 
TCCGCGAGGG AGGAGGCCTG TGTCCGGATC CAGCAGGCGC TCACCATGTT CCCCGGAGTG 
CTCATGCCCC TGCTCGAGTA CTGCAGTGTA CGCCCCGACA CCACCGTGGC CACGCACCAC 
TTCTTCGGAC CCGACGCCGA AATCAGCCAG CCACCCGCCC TGAGCCAGCT GGTGAGCCTG 
TACCTGGGGA GGTCACACTT CCTCTGGAAG GAGCCGGCCA CCATGAGCTG GCTGGAGGAG 
AATGTGCACA AGGTTCTACA GGTGGTGGAC GCCGGGGACC CTGCCGTGGA GGCGTGTGAG 
AGCAGGCGCA AGGTGCTGTA CCAGCGGGCG CCCAGGAACA TCCACCGGCA TGTGGTCCTG 
TCTGAGATCA AGGAGGCTGT GGCCGCCCTG CCGCCGGATG TGACCACACA GTCTGTGTTG 
GGCTTCGACC CTCTGCCCCC ACTGGACACC ATCTACTCCT ACGTCAGACC AGAGAGGCTC 
AGCCCCATCA GCCACGGAAA CACCATCGCC CTCTTCTTCC GCTCACTCCT GCCCAACTAC 
ACTGTGGAGG GGGAGCGACC GGAGGACGGG GGGCCCGGGG GTCTGCACCG TGACCAGGGC 
CTGAACAGAC TGATGCTGGC TGTGCGGGAC ATGATGGCCA ACTTCCACTT CCACGACCTG 
GAGGTGCTGC CCGAGGAGCA CCCTGAGGGC GACGGGGAGT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF25 ( XM_010814600.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812902.2 Bos taurus transcription factor 25 (basic helix-loop-helix) (TCF25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa93387
Clone ID Related Accession (Same CDS sequence)	XM_010814600.2
Accession Version	XM_010814600.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	transcription factor 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) and transcript sequence (BC142262.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814600.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 356 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGCGCC GGGCCCTCCG GAGGCTGAGA GGGGAACAGC GCGGCCAGGA GCCCCTCGGG 
CCTGGCGCCC TGCAGTTTGT CCTTCATGAT GACGATGATG TGGAAGAAGA ATGCCCAAAG 
CGGGGTCCAG GTGGCCGGCG CCCCCGGGGC GCAGGGAAGG AGGGAGTCCG AGTCAACAAC 
CGGTTCGAGC TGATAAACAT CGAGGACCTG GACGAGGCAC CCGGGGTGAA TGGGGAGAGG 
ACAGACTGTC TGCTTGTGGA TGCTGTGGTG TCAGGGAACA AGAGGAGGGC CCAGAGCGGG 
CATTCAGAGT CCACCAGGGG CGGCAATGCG GCAGACACAG CGGTTCCCTC AGAACAGTCC 
AATACCTGTA GCAAACTCCG CAAGAAGAAA AGAAAACAGA AAAATAAGAA AAACGCAGGA 
GAAACCTCGC AGAATGGCCT GGAGGACATT GACCGCATCC TGGAGAGGAT CGAGGATGCC 
AGCGGTGCCA GCCGCCCGGG CCCGCCCCCC CTGAGCACGA AGAAGCATGT CCTGTATGTG 
GAGCACAGAC ACTTGAATCC AGACACAGAG CTGAAAAGAT ATTTTGGTGC CAGAGCTGTT 
CTCGGGGAGC AGAGGCCGCG GCAGAGACAG CGCATGTACC CCAAGTGTAC ATGGCTGACC 
ACCCCGAAGA GCACGTGGCC CCGGTACACC AAACCAGGTC TGTCGATGCG GCTGTTGGAA 
TCCAGGAAAG GCCGCTCGGT CTTCGCCTTT GAGCACAGCG AGGAGTACCA ACAGACGCAG 
CACAAGTTCC TGGCCGCCGT CGAGTCCATG GAGCCCAATA ACATTGTGGT CCTGCTCCAG 
TCAAGCCCCT ACCACGTGGA CTCGCTCCTG CAGCTCAGCG ACGCATGCCG CTTTCAGGAG 
GACCAGGAGA CGGCACGCGA CCTCGTGGAG CGTGCGCTGT ACAGCATGGA GTGTGCCTTC 
CACCCCTTGT TCAGCCTCAC CAGTGGGACC TGCCGGCTGG ACTACCGCAG GCCTGAGAAC 
AGGAGCTTCT ACCTGGCCCT CTACAAGCAG ATGAGCTTCC TGGAGAAGCG GGGCTGCCCA 
CGCACGGCGC TGGAGTACTG CAAGCTCATC CTCAGCCTGG AGCCAGACGA AGACCCCCTG 
TGCATGCTGC TGCTAATCGA CCACCTGGCC TTACGAGCGC GGAGCTACGA GTACTTGATC 
CGCCTGTTCG AAGAGTGGGA GGCCCATCGG AACCTGTCCC AGCTCCCAAA CTTTGCCTTC 
TCTGTGCCGC TGGCCTATTT CCTGCTGAGC CAACAGGCAG ACCTCTCCGA GCAGGAGATG 
AGCTCCGCGA GGGAGGAGGC CTGTGTCCGG ATCCAGCAGG CGCTCACCAT GTTCCCCGGA 
GTGCTCATGC CCCTGCTCGA GTACTGCAGT GTACGCCCCG ACACCACCGT GGCCACGCAC 
CACTTCTTCG GACCCGACGC CGAAATCAGC CAGCCACCCG CCCTGAGCCA GCTGGTGAGC 
CTGTACCTGG GGAGGTCACA CTTCCTCTGG AAGGAGCCGG CCACCATGAG CTGGCTGGAG 
GAGAATGTGC ACAAGGTTCT ACAGGTGGTG GACGCCGGGG ACCCTGCCGT GGAGGCGTGT 
GAGAGCAGGC GCAAGGTGCT GTACCAGCGG GCGCCCAGGA ACATCCACCG GCATGTGGTC 
CTGTCTGAGA TCAAGGAGGC TGTGGCCGCC CTGCCGCCGG ATGTGACCAC ACAGTCTGTG 
TTGGGCTTCG ACCCTCTGCC CCCACTGGAC ACCATCTACT CCTACGTCAG ACCAGAGAGG 
CTCAGCCCCA TCAGCCACGG AAACACCATC GCCCTCTTCT TCCGCTCACT CCTGCCCAAC 
TACACTGTGG AGGGGGAGCG ACCGGAGGAC GGGGGGCCCG GGGGTCTGCA CCGTGACCAG 
GGCCTGAACA GACTGATGCT GGCTGTGCGG GACATGATGG CCAACTTCCA CTTCCACGAC 
CTGGAGGTGC TGCCCGAGGA GCACCCTGAG GGCGACGGGG AGTGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812902.2
CDS	92..2122
Translation	MSRRALRRLRGEQRGQEPLGPGALQFVLHDDDDVEEECPKRGPGGRRPRGAGKEGVRVNNRFELINIEDLDEAPGVNGERTDCLLVDAVVSGNKRRAQSGHSESTRGGNAADTAVPSEQSNTCSKLRKKKRKQKNKKNAGETSQNGLEDIDRILERIEDASGASRPGPPPLSTKKHVLYVEHRHLNPDTELKRYFGARAVLGEQRPRQRQRMYPKCTWLTTPKSTWPRYTKPGLSMRLLESRKGRSVFAFEHSEEYQQTQHKFLAAVESMEPNNIVVLLQSSPYHVDSLLQLSDACRFQEDQETARDLVERALYSMECAFHPLFSLTSGTCRLDYRRPENRSFYLALYKQMSFLEKRGCPRTALEYCKLILSLEPDEDPLCMLLLIDHLALRARSYEYLIRLFEEWEAHRNLSQLPNFAFSVPLAYFLLSQQADLSEQEMSSAREEACVRIQQALTMFPGVLMPLLEYCSVRPDTTVATHHFFGPDAEISQPPALSQLVSLYLGRSHFLWKEPATMSWLEENVHKVLQVVDAGDPAVEACESRRKVLYQRAPRNIHRHVVLSEIKEAVAALPPDVTTQSVLGFDPLPPLDTIYSYVRPERLSPISHGNTIALFFRSLLPNYTVEGERPEDGGPGGLHRDQGLNRLMLAVRDMMANFHFHDLEVLPEEHPEGDGEWD

Target ORF information:

RefSeq Version	XM_010814600.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 25 (basic helix-loop-helix) (TCF25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814600.2

ORF Insert Sequence:

ATGTCGCGCC GGGCCCTCCG GAGGCTGAGA GGGGAACAGC GCGGCCAGGA GCCCCTCGGG 
CCTGGCGCCC TGCAGTTTGT CCTTCATGAT GACGATGATG TGGAAGAAGA ATGCCCAAAG 
CGGGGTCCAG GTGGCCGGCG CCCCCGGGGC GCAGGGAAGG AGGGAGTCCG AGTCAACAAC 
CGGTTCGAGC TGATAAACAT CGAGGACCTG GACGAGGCAC CCGGGGTGAA TGGGGAGAGG 
ACAGACTGTC TGCTTGTGGA TGCTGTGGTG TCAGGGAACA AGAGGAGGGC CCAGAGCGGG 
CATTCAGAGT CCACCAGGGG CGGCAATGCG GCAGACACAG CGGTTCCCTC AGAACAGTCC 
AATACCTGTA GCAAACTCCG CAAGAAGAAA AGAAAACAGA AAAATAAGAA AAACGCAGGA 
GAAACCTCGC AGAATGGCCT GGAGGACATT GACCGCATCC TGGAGAGGAT CGAGGATGCC 
AGCGGTGCCA GCCGCCCGGG CCCGCCCCCC CTGAGCACGA AGAAGCATGT CCTGTATGTG 
GAGCACAGAC ACTTGAATCC AGACACAGAG CTGAAAAGAT ATTTTGGTGC CAGAGCTGTT 
CTCGGGGAGC AGAGGCCGCG GCAGAGACAG CGCATGTACC CCAAGTGTAC ATGGCTGACC 
ACCCCGAAGA GCACGTGGCC CCGGTACACC AAACCAGGTC TGTCGATGCG GCTGTTGGAA 
TCCAGGAAAG GCCGCTCGGT CTTCGCCTTT GAGCACAGCG AGGAGTACCA ACAGACGCAG 
CACAAGTTCC TGGCCGCCGT CGAGTCCATG GAGCCCAATA ACATTGTGGT CCTGCTCCAG 
TCAAGCCCCT ACCACGTGGA CTCGCTCCTG CAGCTCAGCG ACGCATGCCG CTTTCAGGAG 
GACCAGGAGA CGGCACGCGA CCTCGTGGAG CGTGCGCTGT ACAGCATGGA GTGTGCCTTC 
CACCCCTTGT TCAGCCTCAC CAGTGGGACC TGCCGGCTGG ACTACCGCAG GCCTGAGAAC 
AGGAGCTTCT ACCTGGCCCT CTACAAGCAG ATGAGCTTCC TGGAGAAGCG GGGCTGCCCA 
CGCACGGCGC TGGAGTACTG CAAGCTCATC CTCAGCCTGG AGCCAGACGA AGACCCCCTG 
TGCATGCTGC TGCTAATCGA CCACCTGGCC TTACGAGCGC GGAGCTACGA GTACTTGATC 
CGCCTGTTCG AAGAGTGGGA GGCCCATCGG AACCTGTCCC AGCTCCCAAA CTTTGCCTTC 
TCTGTGCCGC TGGCCTATTT CCTGCTGAGC CAACAGGCAG ACCTCTCCGA GCAGGAGATG 
AGCTCCGCGA GGGAGGAGGC CTGTGTCCGG ATCCAGCAGG CGCTCACCAT GTTCCCCGGA 
GTGCTCATGC CCCTGCTCGA GTACTGCAGT GTACGCCCCG ACACCACCGT GGCCACGCAC 
CACTTCTTCG GACCCGACGC CGAAATCAGC CAGCCACCCG CCCTGAGCCA GCTGGTGAGC 
CTGTACCTGG GGAGGTCACA CTTCCTCTGG AAGGAGCCGG CCACCATGAG CTGGCTGGAG 
GAGAATGTGC ACAAGGTTCT ACAGGTGGTG GACGCCGGGG ACCCTGCCGT GGAGGCGTGT 
GAGAGCAGGC GCAAGGTGCT GTACCAGCGG GCGCCCAGGA ACATCCACCG GCATGTGGTC 
CTGTCTGAGA TCAAGGAGGC TGTGGCCGCC CTGCCGCCGG ATGTGACCAC ACAGTCTGTG 
TTGGGCTTCG ACCCTCTGCC CCCACTGGAC ACCATCTACT CCTACGTCAG ACCAGAGAGG 
CTCAGCCCCA TCAGCCACGG AAACACCATC GCCCTCTTCT TCCGCTCACT CCTGCCCAAC 
TACACTGTGG AGGGGGAGCG ACCGGAGGAC GGGGGGCCCG GGGGTCTGCA CCGTGACCAG 
GGCCTGAACA GACTGATGCT GGCTGTGCGG GACATGATGG CCAACTTCCA CTTCCACGAC 
CTGGAGGTGC TGCCCGAGGA GCACCCTGAG GGCGACGGGG AGTGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCF25 ( XM_015467326.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322812.1 Bos taurus transcription factor 25 (basic helix-loop-helix) (TCF25), transcript variant X2, mRNA.

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CloneID	OBa94969
Clone ID Related Accession (Same CDS sequence)	XM_015467326.1
Accession Version	XM_015467326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	transcription factor 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) and transcript sequence (BC142262.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 283 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGCGCC GGGCCCTCCG GAGGCTGAGA GGGGAACAGC GCGGCCAGGA GCCCCTCGGG 
CCTGGCGCCC TGCAGTTTGT CCTTCATGAT GACGATGATG TGGAAGAAGA ATGCCCAAAG 
CGGGGTCCAG GTGGCCGGCG CCCCCGGGGC GCAGGGAAGG AGGGAGTCCG AGTCAACAAC 
CGGTTCGAGC TGATAAACAT CGAGGACCTG GACGAGGCAC CCGGGGTGAA TGGGGAGAGG 
ACAGACTGTC TGCTTGTGGA TGCTGTGGTG TCAGGGAACA AGAGGAGGGC CCAGAGCGGG 
CATTCAGAGT CCACCAGGGG CGGCAATGCG GCAGACACAG CGGTTCCCTC AGAACAGTCC 
AATACCTGTA GCAAACTCCG CAAGAAGAAA AGAAAACAGA AAAATAAGAA AAACGCAGGA 
GAAACCTCGC AGAATGGCCT GGAGGACATT GACCGCATCC TGGAGAGGAT CGAGGATGCC 
AGCGGTGCCA GCCGCCCGGG CCCGCCCCCC CTGAGCACGA AGAAGCATGT CCTGTATGTG 
GAGCACAGAC ACTTGAATCC AGACACAGAG CTGAAAAGAT ATTTTGGTGC CAGAGCTGTT 
CTCGGGGAGC AGAGGCCGCG GCAGAGACAG CGCATGTACC CCAAGTGTAC ATGGCTGACC 
ACCCCGAAGA GCACGTGGCC CCGGTACACC AAACCAGGTC TGTCGATGCG GCTGTTGGAA 
TCCAGGAAAG GCCGCTCGGT CTTCGCCTTT GAGCACAGCG AGGAGTACCA ACAGACGCAG 
CACAAGTTCC TGGCCGCCGT CGAGTCCATG GAGCCCAATA ACATTGTGGT CCTGCTCCAG 
TCAAGCCCCT ACCACGTGGA CTCGCTCCTG CAGCTCAGCG ACGCATGCCG CTTTCAGGAG 
GACCAGGAGA CGGCACGCGA CCTCGTGGAG CGTGCGCTGT ACAGCATGGA GTGTGCCTTC 
CACCCCTTGT TCAGCCTCAC CAGTGGGACC TGCCGGCTGG ACTACCGCAG GCCTGAGAAC 
AGGAGCTTCT ACCTGGCCCT CTACAAGCAG ATGAGCTTCC TGGAGAAGCG GGGCTGCCCA 
CGCACGGCGC TGGAGTACTG CAAGCTCATC CTCAGCCTGG AGCCAGACGA AGACCCCCTG 
TGCATGCTGC TGCTAATCGA CCACCTGGCC TTACGAGCGC GGAGCTACGA GTACTTGATC 
CGCCTGTTCG AAGAGTGGGA GGCCCATCGG AACCTGTCCC AGCTCCCAAA CTTTGCCTTC 
TCTGTGCCGC TGGCCTATTT CCTGCTGAGC CAACAGGCAG ACCTCTCCGA GCAGGAGATG 
AGCTCCGCGA GGGAGGAGGC CTGTGTCCGG ATCCAGCAGG CGCTCACCAT GTTCCCCGGA 
GTGCTCATGC CCCTGCTCGA GTACTGCAGT GTACGCCCCG ACACCACCGT GGCCACGCAC 
CACTTCTTCG GACCCGACGC CGAAATCAGC CAGCCACCCG CCCTGAGCCA GCTGGTGAGC 
CTGTACCTGG GGAGGTCACA CTTCCTCTGG AAGGAGCCGG CCACCATGAG CTGGCTGGAG 
GAGAATGTGC ACAAGGTTCT ACAGGTGGTG GACGCCGGGG ACCCTGCCGT GGAGGCGTGT 
GAGAGCAGGC GCAAGGTGCT GTACCAGCGG GCGCCCAGGA ACATCCACCG GCATGTGGTC 
CTGTCTGAGA TCAAGGAGGC TGTGGCCGCC CTGCCGCCGG ATGTGACCAC ACAGTCTGTG 
TTGGGCTTCG ACCCTCTGCC CCCACTGGAC ACCATCTACT CCTACGTCAG ACCAGAGAGG 
CTCAGCCCCA TCAGCCACGG AAACACCATC GCCCCTCTCT TCCGCTCACT CCTGCCCAAC 
TACACTGTGG AGGGGGAGCG ACCGGAGGAC GGGGGGCCCG GGGGTCTGCA CCGTGACCAG 
GGCCTGAACA GACTGATGCT GGCTGTGCGG GACATGATGG CCAACTTCCA CTTCCACGAC 
CTGGAGGTGC TGCCCGAGGA GCACCCTGAG GGCGACGGGG AGTGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322812.1
CDS	92..2122
Translation	MSRRALRRLRGEQRGQEPLGPGALQFVLHDDDDVEEECPKRGPGGRRPRGAGKEGVRVNNRFELINIEDLDEAPGVNGERTDCLLVDAVVSGNKRRAQSGHSESTRGGNAADTAVPSEQSNTCSKLRKKKRKQKNKKNAGETSQNGLEDIDRILERIEDASGASRPGPPPLSTKKHVLYVEHRHLNPDTELKRYFGARAVLGEQRPRQRQRMYPKCTWLTTPKSTWPRYTKPGLSMRLLESRKGRSVFAFEHSEEYQQTQHKFLAAVESMEPNNIVVLLQSSPYHVDSLLQLSDACRFQEDQETARDLVERALYSMECAFHPLFSLTSGTCRLDYRRPENRSFYLALYKQMSFLEKRGCPRTALEYCKLILSLEPDEDPLCMLLLIDHLALRARSYEYLIRLFEEWEAHRNLSQLPNFAFSVPLAYFLLSQQADLSEQEMSSAREEACVRIQQALTMFPGVLMPLLEYCSVRPDTTVATHHFFGPDAEISQPPALSQLVSLYLGRSHFLWKEPATMSWLEENVHKVLQVVDAGDPAVEACESRRKVLYQRAPRNIHRHVVLSEIKEAVAALPPDVTTQSVLGFDPLPPLDTIYSYVRPERLSPISHGNTIAPLFRSLLPNYTVEGERPEDGGPGGLHRDQGLNRLMLAVRDMMANFHFHDLEVLPEEHPEGDGEWD

Target ORF information:

RefSeq Version	XM_015467326.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transcription factor 25 (basic helix-loop-helix) (TCF25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015467326.1

ORF Insert Sequence:

ATGTCGCGCC GGGCCCTCCG GAGGCTGAGA GGGGAACAGC GCGGCCAGGA GCCCCTCGGG 
CCTGGCGCCC TGCAGTTTGT CCTTCATGAT GACGATGATG TGGAAGAAGA ATGCCCAAAG 
CGGGGTCCAG GTGGCCGGCG CCCCCGGGGC GCAGGGAAGG AGGGAGTCCG AGTCAACAAC 
CGGTTCGAGC TGATAAACAT CGAGGACCTG GACGAGGCAC CCGGGGTGAA TGGGGAGAGG 
ACAGACTGTC TGCTTGTGGA TGCTGTGGTG TCAGGGAACA AGAGGAGGGC CCAGAGCGGG 
CATTCAGAGT CCACCAGGGG CGGCAATGCG GCAGACACAG CGGTTCCCTC AGAACAGTCC 
AATACCTGTA GCAAACTCCG CAAGAAGAAA AGAAAACAGA AAAATAAGAA AAACGCAGGA 
GAAACCTCGC AGAATGGCCT GGAGGACATT GACCGCATCC TGGAGAGGAT CGAGGATGCC 
AGCGGTGCCA GCCGCCCGGG CCCGCCCCCC CTGAGCACGA AGAAGCATGT CCTGTATGTG 
GAGCACAGAC ACTTGAATCC AGACACAGAG CTGAAAAGAT ATTTTGGTGC CAGAGCTGTT 
CTCGGGGAGC AGAGGCCGCG GCAGAGACAG CGCATGTACC CCAAGTGTAC ATGGCTGACC 
ACCCCGAAGA GCACGTGGCC CCGGTACACC AAACCAGGTC TGTCGATGCG GCTGTTGGAA 
TCCAGGAAAG GCCGCTCGGT CTTCGCCTTT GAGCACAGCG AGGAGTACCA ACAGACGCAG 
CACAAGTTCC TGGCCGCCGT CGAGTCCATG GAGCCCAATA ACATTGTGGT CCTGCTCCAG 
TCAAGCCCCT ACCACGTGGA CTCGCTCCTG CAGCTCAGCG ACGCATGCCG CTTTCAGGAG 
GACCAGGAGA CGGCACGCGA CCTCGTGGAG CGTGCGCTGT ACAGCATGGA GTGTGCCTTC 
CACCCCTTGT TCAGCCTCAC CAGTGGGACC TGCCGGCTGG ACTACCGCAG GCCTGAGAAC 
AGGAGCTTCT ACCTGGCCCT CTACAAGCAG ATGAGCTTCC TGGAGAAGCG GGGCTGCCCA 
CGCACGGCGC TGGAGTACTG CAAGCTCATC CTCAGCCTGG AGCCAGACGA AGACCCCCTG 
TGCATGCTGC TGCTAATCGA CCACCTGGCC TTACGAGCGC GGAGCTACGA GTACTTGATC 
CGCCTGTTCG AAGAGTGGGA GGCCCATCGG AACCTGTCCC AGCTCCCAAA CTTTGCCTTC 
TCTGTGCCGC TGGCCTATTT CCTGCTGAGC CAACAGGCAG ACCTCTCCGA GCAGGAGATG 
AGCTCCGCGA GGGAGGAGGC CTGTGTCCGG ATCCAGCAGG CGCTCACCAT GTTCCCCGGA 
GTGCTCATGC CCCTGCTCGA GTACTGCAGT GTACGCCCCG ACACCACCGT GGCCACGCAC 
CACTTCTTCG GACCCGACGC CGAAATCAGC CAGCCACCCG CCCTGAGCCA GCTGGTGAGC 
CTGTACCTGG GGAGGTCACA CTTCCTCTGG AAGGAGCCGG CCACCATGAG CTGGCTGGAG 
GAGAATGTGC ACAAGGTTCT ACAGGTGGTG GACGCCGGGG ACCCTGCCGT GGAGGCGTGT 
GAGAGCAGGC GCAAGGTGCT GTACCAGCGG GCGCCCAGGA ACATCCACCG GCATGTGGTC 
CTGTCTGAGA TCAAGGAGGC TGTGGCCGCC CTGCCGCCGG ATGTGACCAC ACAGTCTGTG 
TTGGGCTTCG ACCCTCTGCC CCCACTGGAC ACCATCTACT CCTACGTCAG ACCAGAGAGG 
CTCAGCCCCA TCAGCCACGG AAACACCATC GCCCCTCTCT TCCGCTCACT CCTGCCCAAC 
TACACTGTGG AGGGGGAGCG ACCGGAGGAC GGGGGGCCCG GGGGTCTGCA CCGTGACCAG 
GGCCTGAACA GACTGATGCT GGCTGTGCGG GACATGATGG CCAACTTCCA CTTCCACGAC 
CTGGAGGTGC TGCCCGAGGA GCACCCTGAG GGCGACGGGG AGTGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Unexpected cross-species contamination in genome sequencing projects.
PeerJ2e675(2014)
Merchant S,Wood DE,Salzberg SL

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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TCF25 cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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