PPO2 cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPO2
Entrez Gene ID	693073
Full Name	phenoloxidase subunit 2
Synonyms	BmPPO2,pro-PO2
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044069.1	NP_001037534.1	phenoloxidase subunit 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A QM protein from Bombyx mori negatively regulates prophenoloxidase activation and melanization by interacting with Jun protein.
Zhou XS, Chen C, Li TH, Tang JJ, Zhu BJ, Wei GQ, Qian C, Liu CL, Wang L
Insect molecular biology28(4)578-590(2019 08)

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
Suetsugu Y, Futahashi R, Kanamori H, Kadono-Okuda K, Sasanuma S, Narukawa J, Ajimura M, Jouraku A, Namiki N, Shimomura M, Sezutsu H, Osanai-Futahashi M, Suzuki MG, Daimon T, Shinoda T, Taniai K, Asaoka K, Niwa R, Kawaoka S, Katsuma S, Tamura T, Noda H, Kasahara M, Sugano S, Suzuki Y, Fujiwara H, Kataoka H, Arunkumar KP, Tomar A, Nagaraju J, Goldsmith MR, Feng Q, Xia Q, Yamamoto K, Shimada T, Mita K
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)

A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori.
Tanaka H, Ishibashi J, Fujita K, Nakajima Y, Sagisaka A, Tomimoto K, Suzuki N, Yoshiyama M, Kaneko Y, Iwasaki T, Sunagawa T, Yamaji K, Asaoka A, Mita K, Yamakawa M
Insect biochemistry and molecular biology38(12)1087-110(2008 Dec)

Cuticular pro-phenoloxidase of the silkworm, Bombyx mori. Purification and demonstration of its transport from hemolymph.
Asano T, Ashida M
The Journal of biological chemistry276(14)11100-12(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PPO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBg00028	NM_001044069.1 Latest version!	Bombyx mori phenoloxidase subunit 2 (PPO2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPO2 ( NM_001044069.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001037534.1 Bombyx mori phenoloxidase subunit 2 (PPO2), mRNA.

Price & Availability↑

CloneID	OBg00028
Clone ID Related Accession (Same CDS sequence)	NM_001044069.1
Accession Version	NM_001044069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Bombyx mori(domestic silkworm)
Product	phenoloxidase subunit 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D49371.1. On Sep 6, 2006 this sequence version replaced NM_001044070.1. ##Evidence-Data-START## Transcript exon combination :: D49371.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTGACG TTTTTGAAAG CCTCGAGTTG CTGTTCGATC GTCCAAATGA GCCGCTCATC 
ACACCCAAGG GCGAAAACAA TTCTGTGTTT CAACTCACAG AACAATTTTT GACTGAGGAC 
TACGCCAACA ACGGCATCGA ATTAAACAAC CGTTTCGGTG ACGATGCTTC TGAGAAGATA 
CCCCTCAAGA ACCTCAGCAA ACTCCCAGAA TTTAAAATTG CAACTCAACT ACCCAAGGAC 
GCTGAATTCT CATTGTTTCT ACCTAAACAT CAAGAAATGG CAAATGAACT TCTTGGCGTT 
CTCATGGATG TACCAGAGAA CGAATTACAA GATTTGCTAT CGACATGCGC CTTTGCACGA 
GTAAACCTGA ACCCTCAGTT GTTCAACTAT TGTTACTCTG TGGCACTGAT GCACAGACGT 
GATACCAGAA AAGTCAGAGT TAAGAATTTT GCAGAAGTAT TTCCCTCTAA ATTCTTGGAT 
TCCCAAGTAT TCACTCAGGC TCGTGAAACT GCAGCCGTTA TTCCACCAGA TGTTCCACGC 
ATACCTATTA TCATTCCACG AGACTACACT GCGACGGACT TAGAAGAAGA ACACCGCCTT 
GCGTACTGGC GAGAAGATAT CGGCATCAAC CTGCATCATT ATCACTGGCA TTTGGTCTAC 
CCGTTTACGG CCAACGATCT TTCAATCGTA GCTAAGGACC GTCGTGGGGA GTTGTTCTTC 
TATATGCACC AGCAAGTGAT AGCCCGCTTC AACTGCGAGC GCTTGTGCAA TTCATTAAAA 
AGAGTTAAAA AATTCAGCAA CTGGAGGGAG CCAATTCCAG AGGCTTACTT CCCTAAGCTT 
GACAGTTTGA CTTCGTCGCG CGGATGGCCG CCGCGTCAGT CCGGTATGCA ATGGCAGGAC 
CTGAATCGTG CGGCCGAAGG TCTATTTGTG ACCATTGACG AAATGGAACG CTGGAGAAGA 
AACGTTGAAG AAGCTATCGC GACTGGTACC GTTAGATTGC CAAACGGGCA GACTCGGCCT 
CTTGACATCG ATACGCTCGG CAACATGTTG GAGTCCAGCG CCCTCTCGCC GAACAGAGAA 
CTGTACGGTT CGATACACAA CAACGGGCAC AGTTTCACCG CGTACATGCA CGACCCGGAA 
CACAGATACC TTGAACAATT TGGTGTTATC GCTGATGAGG CCACAACGAT GCGCGATCCA 
TTCTTCTACC GCTGGCACGC CTACATCGAT GACGTTTTCC AGAAGCACAA GGAATCTGCC 
TACGTGCGTC CTTACACTCG ATCTGAGCTC GAGAACCCGG GCGTGCAAGT GAGGTCCGTG 
TCGGTGGAGA CGCCCGGCGG ACAACCGAAC ACGCTGAACA CGTTCTGGAT GCTCAGCGAC 
GTGAACTTGT CGCGCGGACT CGACTTCTCC GACAACGGGC CCGTGTACGC TCGCTTCACT 
CACCTCAACT ACAGGCATTT CAGCTACAGA ATAAACGTGA ACAACACCGG TAGCAGCCGT 
CGCACCACAG TGCGTATCTT CATCACTCCG AAGTTCGACG AGCGCAATGT CCCCTGGATA 
TTCTCGGACC AACGCAAGAT GTGTATTGAA ATGGACAGAT TCGTCACTGT CCTGAACGCC 
GGAGAGAATA ATATTGTCCG TCAGTCGACG GAGTCTTCCA TAACTATTCC GTTCGAACAG 
ACATTCCGCG ACCTATCCGC TCAGGGCAAT GATCCACGAC GCGACGAACT CGCTACCTTC 
AATTACTGCG GCTGCGGCTG GCCTCAGCAC ATGCTCGTGC CCAAGGGCAC TGAAGCCGGC 
ATGCCCTTCC AACTGTTTGT TATGTTATCC AACTATGATT TAGACCGGAT CGATCAAGAT 
GACGGAAAAC AGCTCACTTG TGTGGAAGCG TCGAGCTTCT GTGGATTAAA GGATAAGAAA 
TATCCTGATA GGCGCGCTAT GGGATTCCCG TTCGATCGAC CCTCGAGCAG CGCCACCAGC 
CTCCAGGACT TCATTTTACC AAACATGGGC TTGCAGGACA TCACTATTCA ATTGCAAAAC 
GTCACCGAAC CTAACCCACG GAACCCTCCC ATGTCTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037534.1
CDS	45..2126
Translation	MADVFESLELLFDRPNEPLITPKGENNSVFQLTEQFLTEDYANNGIELNNRFGDDASEKIPLKNLSKLPEFKIATQLPKDAEFSLFLPKHQEMANELLGVLMDVPENELQDLLSTCAFARVNLNPQLFNYCYSVALMHRRDTRKVRVKNFAEVFPSKFLDSQVFTQARETAAVIPPDVPRIPIIIPRDYTATDLEEEHRLAYWREDIGINLHHYHWHLVYPFTANDLSIVAKDRRGELFFYMHQQVIARFNCERLCNSLKRVKKFSNWREPIPEAYFPKLDSLTSSRGWPPRQSGMQWQDLNRAAEGLFVTIDEMERWRRNVEEAIATGTVRLPNGQTRPLDIDTLGNMLESSALSPNRELYGSIHNNGHSFTAYMHDPEHRYLEQFGVIADEATTMRDPFFYRWHAYIDDVFQKHKESAYVRPYTRSELENPGVQVRSVSVETPGGQPNTLNTFWMLSDVNLSRGLDFSDNGPVYARFTHLNYRHFSYRINVNNTGSSRRTTVRIFITPKFDERNVPWIFSDQRKMCIEMDRFVTVLNAGENNIVRQSTESSITIPFEQTFRDLSAQGNDPRRDELATFNYCGCGWPQHMLVPKGTEAGMPFQLFVMLSNYDLDRIDQDDGKQLTCVEASSFCGLKDKKYPDRRAMGFPFDRPSSSATSLQDFILPNMGLQDITIQLQNVTEPNPRNPPMSV

Target ORF information:

RefSeq Version	NM_001044069.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori phenoloxidase subunit 2 (PPO2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044069.1

ORF Insert Sequence:

ATGGCTGACG TTTTTGAAAG CCTCGAGTTG CTGTTCGATC GTCCAAATGA GCCGCTCATC 
ACACCCAAGG GCGAAAACAA TTCTGTGTTT CAACTCACAG AACAATTTTT GACTGAGGAC 
TACGCCAACA ACGGCATCGA ATTAAACAAC CGTTTCGGTG ACGATGCTTC TGAGAAGATA 
CCCCTCAAGA ACCTCAGCAA ACTCCCAGAA TTTAAAATTG CAACTCAACT ACCCAAGGAC 
GCTGAATTCT CATTGTTTCT ACCTAAACAT CAAGAAATGG CAAATGAACT TCTTGGCGTT 
CTCATGGATG TACCAGAGAA CGAATTACAA GATTTGCTAT CGACATGCGC CTTTGCACGA 
GTAAACCTGA ACCCTCAGTT GTTCAACTAT TGTTACTCTG TGGCACTGAT GCACAGACGT 
GATACCAGAA AAGTCAGAGT TAAGAATTTT GCAGAAGTAT TTCCCTCTAA ATTCTTGGAT 
TCCCAAGTAT TCACTCAGGC TCGTGAAACT GCAGCCGTTA TTCCACCAGA TGTTCCACGC 
ATACCTATTA TCATTCCACG AGACTACACT GCGACGGACT TAGAAGAAGA ACACCGCCTT 
GCGTACTGGC GAGAAGATAT CGGCATCAAC CTGCATCATT ATCACTGGCA TTTGGTCTAC 
CCGTTTACGG CCAACGATCT TTCAATCGTA GCTAAGGACC GTCGTGGGGA GTTGTTCTTC 
TATATGCACC AGCAAGTGAT AGCCCGCTTC AACTGCGAGC GCTTGTGCAA TTCATTAAAA 
AGAGTTAAAA AATTCAGCAA CTGGAGGGAG CCAATTCCAG AGGCTTACTT CCCTAAGCTT 
GACAGTTTGA CTTCGTCGCG CGGATGGCCG CCGCGTCAGT CCGGTATGCA ATGGCAGGAC 
CTGAATCGTG CGGCCGAAGG TCTATTTGTG ACCATTGACG AAATGGAACG CTGGAGAAGA 
AACGTTGAAG AAGCTATCGC GACTGGTACC GTTAGATTGC CAAACGGGCA GACTCGGCCT 
CTTGACATCG ATACGCTCGG CAACATGTTG GAGTCCAGCG CCCTCTCGCC GAACAGAGAA 
CTGTACGGTT CGATACACAA CAACGGGCAC AGTTTCACCG CGTACATGCA CGACCCGGAA 
CACAGATACC TTGAACAATT TGGTGTTATC GCTGATGAGG CCACAACGAT GCGCGATCCA 
TTCTTCTACC GCTGGCACGC CTACATCGAT GACGTTTTCC AGAAGCACAA GGAATCTGCC 
TACGTGCGTC CTTACACTCG ATCTGAGCTC GAGAACCCGG GCGTGCAAGT GAGGTCCGTG 
TCGGTGGAGA CGCCCGGCGG ACAACCGAAC ACGCTGAACA CGTTCTGGAT GCTCAGCGAC 
GTGAACTTGT CGCGCGGACT CGACTTCTCC GACAACGGGC CCGTGTACGC TCGCTTCACT 
CACCTCAACT ACAGGCATTT CAGCTACAGA ATAAACGTGA ACAACACCGG TAGCAGCCGT 
CGCACCACAG TGCGTATCTT CATCACTCCG AAGTTCGACG AGCGCAATGT CCCCTGGATA 
TTCTCGGACC AACGCAAGAT GTGTATTGAA ATGGACAGAT TCGTCACTGT CCTGAACGCC 
GGAGAGAATA ATATTGTCCG TCAGTCGACG GAGTCTTCCA TAACTATTCC GTTCGAACAG 
ACATTCCGCG ACCTATCCGC TCAGGGCAAT GATCCACGAC GCGACGAACT CGCTACCTTC 
AATTACTGCG GCTGCGGCTG GCCTCAGCAC ATGCTCGTGC CCAAGGGCAC TGAAGCCGGC 
ATGCCCTTCC AACTGTTTGT TATGTTATCC AACTATGATT TAGACCGGAT CGATCAAGAT 
GACGGAAAAC AGCTCACTTG TGTGGAAGCG TCGAGCTTCT GTGGATTAAA GGATAAGAAA 
TATCCTGATA GGCGCGCTAT GGGATTCCCG TTCGATCGAC CCTCGAGCAG CGCCACCAGC 
CTCCAGGACT TCATTTTACC AAACATGGGC TTGCAGGACA TCACTATTCA ATTGCAAAAC 
GTCACCGAAC CTAACCCACG GAACCCTCCC ATGTCTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A QM protein from Bombyx mori negatively regulates prophenoloxidase activation and melanization by interacting with Jun protein.
Insect molecular biology28(4)578-590(2019 08)
Zhou XS,Chen C,Li TH,Tang JJ,Zhu BJ,Wei GQ,Qian C,Liu CL,Wang L

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)
Suetsugu Y,Futahashi R,Kanamori H,Kadono-Okuda K,Sasanuma S,Narukawa J,Ajimura M,Jouraku A,Namiki N,Shimomura M,Sezutsu H,Osanai-Futahashi M,Suzuki MG,Daimon T,Shinoda T,Taniai K,Asaoka K,Niwa R,Kawaoka S,Katsuma S,Tamura T,Noda H,Kasahara M,Sugano S,Suzuki Y,Fujiwara H,Kataoka H,Arunkumar KP,Tomar A,Nagaraju J,Goldsmith MR,Feng Q,Xia Q,Yamamoto K,Shimada T,Mita K

A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori.
Insect biochemistry and molecular biology38(12)1087-110(2008 Dec)
Tanaka H,Ishibashi J,Fujita K,Nakajima Y,Sagisaka A,Tomimoto K,Suzuki N,Yoshiyama M,Kaneko Y,Iwasaki T,Sunagawa T,Yamaji K,Asaoka A,Mita K,Yamakawa M

Cuticular pro-phenoloxidase of the silkworm, Bombyx mori. Purification and demonstration of its transport from hemolymph.
The Journal of biological chemistry276(14)11100-12(2001 Apr)
Asano T,Ashida M

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PPO2 cDNA ORF clone, Bombyx mori(domestic silkworm)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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