Dnmt1 cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnmt1
Entrez Gene ID	692528
Full Name	DNA cytosine-5 methyltransferase
Synonyms	BmDnmt1
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043515.2	NP_001036980.1	DNA cytosine-5 methyltransferase
XM_012695406.1	XP_012550860.1	DNA cytosine-5 methyltransferase isoform X1
XM_012695406.2	XP_012550860.1	DNA cytosine-5 methyltransferase isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

DNA methyltransferase BmDnmt1 and BmDnmt2 in silkworm (Bombyx mori) and the regulation of silkworm embryonic development.
Li B, Hu P, Zhang SZ, Toufeeq S, Wang J, Zhao K, Xu X, Xu JP, Huang SJ
Archives of insect biochemistry and physiology100(3)e21529(2019 Mar)

DNA methylation mediates BmDeaf1-regulated tissue- and stage-specific expression of BmCHSA-2b in the silkworm, Bombyx mori.
Xu G, Zhang J, Lyu H, Song Q, Feng Q, Xiang H, Zheng S
Epigenetics & chromatin11(1)32(2018 06)

Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori.
Mitsudome T, Mon H, Xu J, Li Z, Lee JM, Patil AA, Masuda A, Iiyama K, Morokuma D, Kusakabe T
Insect biochemistry and molecular biology5855-65(2015 Mar)

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dnmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBg13491	NM_001043515.2 Latest version!	Bombyx mori DNA cytosine-5 methyltransferase (Dnmt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBg13919	XM_012695406.2 Latest version!	Bombyx mori DNA cytosine-5 methyltransferase (Dnmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OBg13919	XM_012695406.1	Bombyx mori DNA cytosine-5 methyltransferase (Dnmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnmt1 ( NM_001043515.2 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001036980.1 Bombyx mori DNA cytosine-5 methyltransferase (Dnmt1), mRNA.

Price & Availability↑

CloneID	OBg13491
Clone ID Related Accession (Same CDS sequence)	NM_001043515.2
Accession Version	NM_001043515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550246400000
Organism	Bombyx mori(domestic silkworm)
Product	DNA cytosine-5 methyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB194008.1. On Dec 1, 2014 this sequence version replaced NM_001043515.1. ##Evidence-Data-START## Transcript exon combination :: AB194008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03699706 [ECO:0000348] ##Evidence-Data-END##

ATGCCCACTT CAACTATTAC TTGCAACAGT ATAACACAAG TGTGCATGGA TAAGATACTT 
GATGGGGATG AAATAATCAA AGAAAACTCT AAGAGAAAAA GGAGCTGTGA AGAAATTGTT 
TCTGCCAATA ATAAACGATC AAAAACAGAA GACAATGATG GACATCAAAT TTCACAAGAT 
TCTACAAGCA ATGACTCTAT TGAAAATCTT AACATTCCAG CAAAAAATAC TAGTCTTATT 
GTTTCTGAAA ACATCAATAA TGTATATAAA AATTATGACG AGGTTGAAAA TAAACCAGTA 
TATAAAAATG GTGACCCTAT CGTTGTTGAA ATAGATAACA AGGATAAGCC TGGATCAGTT 
AACAACAATC ATGACATGAT TGATGATTCA GAGACCATAT CTAATATTAA GAATAGCAAT 
AATATTATCC CTGATACAGA GAAATGTAAC ATATGTGGCC AATTTTTAAA TAATTCTGAC 
TTAATTTATT ACCAAGGTCA CCCACAAGAT GCTGTTGAAG AATATATTGC ATTGACTAAT 
GACAAACTTG TTTTGAGCTC TGGGGAAGAT GGAGACATAA TGGAACGACC CCAAACAAAT 
ATAACTGGCT TTACAATATT TGATGAGCAA GGACATTTGT GTCCAATTGA TGGTGGTTTG 
GTAGAAAACG ATGTTCGAAT TTATATGTCT GGATATTTAA AGTCAATTTG CTCTGATTCA 
TCAGAGATTG ACGAAGAATC CATTCCAGTT AAAGATGTAG GTCCTATAAT AGAATGGTTC 
ATACATGGTT TCGATGGTGG AGATAGGAAC TGTATAACTT TATCAACGGA ATTTGGGGAA 
TATAACTTGC TCAAACCTAG TGAAGCTTAT ACGCCCCTAA TGAATAATTT ATATGAAAAA 
ATATGGCTTA GCAAAGTAGT CGTCGAATTC TTAGAAGAAT ATCACTACTT GCAACCGAGC 
TATGAAGATT TACTTGAAGT TGTAAGAGAT TTTTCCATTC CTGAACTAAA TAATAAAAAG 
ATGACAGAAG AAATGCTACA TAAACATGCC CAGTTTGTTT GTGATCAAGT AGTTAGCTTA 
GAAATAGAGG AAGATGATGA ACCATTAATA ACATTACCTT GTATGAGAGA ACTTATCAAA 
TTAATGGGCA TAAAATTCGG TAAACGAAAA ATACGTACCC AGATACAATA CAAAAAAACA 
GATAAGAAAG CATGGACAAA AGCTACAACA ACTCCACTTG TAAGAAAAAC ATTTGAAAGT 
TTTTTCTCAA ATCAGTTAGA CAAAACCAAT CACGAACTGG TCTTACGAAG AAAACGATGC 
GGGGTTTGTG AAGCCTGTCA ATTGCCTGAT TGCGGCGAAT GTAATGCATG TCGTGCCATG 
GCTAAATTCG GTGGTCATGG AAGAACTAAG AAAGCATGCG TCCGTAGATT ATGTCCTAAT 
ATGGCAGTAG AACAGGCAGA AGATTCTGAT CCCGATGATG AAGATGAGTA TCAGCAAATA 
TCTGAGAAAA AACAAGACAA AATAGATGAT GCAGTACCAG TCAAGCTCAC TGGGTCAAAT 
AGTAAAAATT TAAAATGGAT TGGTGAGCCT GTTAAAGCTG ATGCCACAAA AATATATTAC 
GAAAAAGTTG AAATTGATGG TGCTGAGCTG TGTAATGGTG ACTTTGTCAT GATTGAAACT 
TCCCAAACAA ACATTCCTAC ACTGGTCGCT AAAGTTGTAT ACATGTGGAA AGAAATCCAT 
AACCCGAAAT CAGGCTATTT TCACGGAGAA GTCTTCATAC GAGCCTCGGA CACAGTTTTA 
GGAGAAGTGA GTGACCCAAG AGAAGTATTT TTGGGCGACA GATGTTGCCA TGGTGCTCCG 
CTTTCTTCCA TATTAAGAAA AGCGAATATA GAAAGGAAAG AGACGTCAGC AGATTGGTTT 
AAACTTGGCG GGAAAGAAGT AGACGACGAA CATTTCGAGG ATGATGGAAG AACTTATTTT 
TATAGCAAGT ATTACGACAG GTTCACGTCG CGTTTTGAAG ATTTACCTCC AGATCCAGCG 
TGTCCAAATG CTTTAAGAAA GCACAGATTC TGCCCATCTT GTGAACGTAA AACAAAACGA 
GATGCAAGAA ACATTCCTAA AGTTTTTGAA AAATTGATTG TGAAATCCGA AATTGTTTCA 
GAACAAAACA GGTCGGAATG GTCATATGTA AAATGGCAAG ACTTTGACTA TAAGAAGGGT 
TGTGGTGTTT TCCTAAAACC GGGAACCTTT AAACTAAAAA ATAGTATGAC AAAAGCGAAC 
ACTGTTGCCA AACCGCGGTT TGAAAAAGTC GATGAAACTA TTTATCCTGA ATATTATAGG 
AAGAACGATT CTAATACGCG TGGGTCTAAT ATTGATACCG GGGAACCTTT TTGTGTGGGT 
TACATAGCTG CCGTCACTGC CGCGAGTGAG GGACCTCTAG TTGTACCGCA AGATATTTAC 
CTGAAAGTGA ATGTGCTGCT TAGACCTGAA AACACGAGCA GTAAATTTCC GCAGCACGAG 
GACACTAATG TTTTATATTG GACAACTGAA ATAAGGGAGA TTCCATTTTC GACCGTCGTT 
GGGCATTGTC ATTTAATTTA TGAACAAAAT GTTCCCCAAA ATATTTCACT TCAAGAATGG 
TTAGGAAATG ATCCGTGCAG ATTCTACTTC AGAATGGCGT ATTGTAAGTC CACGGGAGAG 
TTCACGGATC TCCCGCAGAA TGCCATCAGT GTTGGAAGAA CAGATCGTAC TAAAGATAAA 
GGGAAGGGGA AGGGAAAATC TACAAAAACT ATTGAAACGG TTCCCGCTAA AGTTGTAGAG 
GAGAAAATTA GACCGTTGCG AACTTTAGAC GTGTTTGCCG GTTGTGGGGG CCTATCAGAA 
GGCTTGCATC AAGCTGGAGT TGCAGAATGC AAGTGGGCTA TTGAAAACGT GGAAGCTGCT 
TCTCATGCTT ATTCATTAAA TAATAAAAGT TGTATTGTAT TTAATGAAGA CTGTAATGCA 
CTTTTGAAAA CTGTAATGTC TGGTGCTAAG CACAGCGCGA ATGGACTGCG GCTCCCCATG 
CAAGGAGAAG TGGAGCTTTT ATGTGGCGGG CCACCATGTC AAGGCTTCTC CGGAATGAAT 
CGTTTTAATT CAAGAGAGTA TTCAAACTTC AAAAACTCAT TAGTTGCATC GTATTTATCG 
TTTTGTGATT ATTACAGACC TAAATATTTT ATACTGGAAA ACGTTCGTAA CTTTGTGGCC 
TTTAAAAAAG GTATGGTTTT GAAACTCACT CTGCGAGCTT TGTTGGACAT GGGATACCAG 
TGTACCTTTG GTATCTTACA AGCGGGTAAC TACGGAGTTC CCCAAACTAG ACGAAGGTTG 
ATCATATTGG CGGCAGCGCC GGGTTACAAT CTTCCGTTCT ACCCAGAACC CACGCACGTC 
TTCAGTAGAC GTGCTTGTAC ATTAACAACG ACGATTGACG GCAAAAGGTT CTCGACTAAT 
ATTCACTGGG ACGAGTCGGC TCCAAAAAGA ACGTGCACCA TTCAAGACGC CATGAGTGAT 
TTGCCTCAAA TATGTAATGG TGCTAATAAA ATAGAAATCG AATATGGCTC CATGCCAGAA 
AGTCATTTCC AGAGATTAGT GCGAAGCAAC GATGAGAATT CTAAACTACG AGATCACATC 
TGTAAAAACA TGGCGCCATT GATTCAAGCT AGGATCAGCA GGATACCTAC AACACCGGGC 
TCCGACTGGA GAGATCTACC CAACATATCC GTTACTCTCT CCGATGGCAC TAAGTGCAAG 
GTTCTACAGT ATCGCTACGA CGACAAGCGC AATGGGCGGT CGAGCAGCGG CGCAGTGCGC 
GGCGTGTGCG CGTGCGCGAG CGGCCGCGCC TGCTCGCCGC TGGACAAGCA GGAGAACACG 
CTCATCCCGT GGTGTCTGCC GCACACCGGC AACAGGCACA ACCACTGGGC CGGGCTCTAC 
GGTCGGCTAT CGTGGGGCGG GTACTTCAGC ACGACAGTGA CGGATCCCGA ACCGATGGGC 
AAGCAAGGCC GGGTGCTCCA CCCCGACCAG CACCGAGTCG TCTCCGTGCG AGAGTGCGCG 
CGCTCGCAGG GCTTCCCTGA TACGTATCTC TTCGCAGGCT CCGTACAGGA CAAACATCGA 
CAGATCGGAA ATGCAGTTCC ACCACCTCTT GGCGCCGCTC TGGGTAGAGA AATAAAGAAA 
GCGCTTACTC TCAGCCTGAC CACATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036980.1
CDS	105..4334
Translation	MPTSTITCNSITQVCMDKILDGDEIIKENSKRKRSCEEIVSANNKRSKTEDNDGHQISQDSTSNDSIENLNIPAKNTSLIVSENINNVYKNYDEVENKPVYKNGDPIVVEIDNKDKPGSVNNNHDMIDDSETISNIKNSNNIIPDTEKCNICGQFLNNSDLIYYQGHPQDAVEEYIALTNDKLVLSSGEDGDIMERPQTNITGFTIFDEQGHLCPIDGGLVENDVRIYMSGYLKSICSDSSEIDEESIPVKDVGPIIEWFIHGFDGGDRNCITLSTEFGEYNLLKPSEAYTPLMNNLYEKIWLSKVVVEFLEEYHYLQPSYEDLLEVVRDFSIPELNNKKMTEEMLHKHAQFVCDQVVSLEIEEDDEPLITLPCMRELIKLMGIKFGKRKIRTQIQYKKTDKKAWTKATTTPLVRKTFESFFSNQLDKTNHELVLRRKRCGVCEACQLPDCGECNACRAMAKFGGHGRTKKACVRRLCPNMAVEQAEDSDPDDEDEYQQISEKKQDKIDDAVPVKLTGSNSKNLKWIGEPVKADATKIYYEKVEIDGAELCNGDFVMIETSQTNIPTLVAKVVYMWKEIHNPKSGYFHGEVFIRASDTVLGEVSDPREVFLGDRCCHGAPLSSILRKANIERKETSADWFKLGGKEVDDEHFEDDGRTYFYSKYYDRFTSRFEDLPPDPACPNALRKHRFCPSCERKTKRDARNIPKVFEKLIVKSEIVSEQNRSEWSYVKWQDFDYKKGCGVFLKPGTFKLKNSMTKANTVAKPRFEKVDETIYPEYYRKNDSNTRGSNIDTGEPFCVGYIAAVTAASEGPLVVPQDIYLKVNVLLRPENTSSKFPQHEDTNVLYWTTEIREIPFSTVVGHCHLIYEQNVPQNISLQEWLGNDPCRFYFRMAYCKSTGEFTDLPQNAISVGRTDRTKDKGKGKGKSTKTIETVPAKVVEEKIRPLRTLDVFAGCGGLSEGLHQAGVAECKWAIENVEAASHAYSLNNKSCIVFNEDCNALLKTVMSGAKHSANGLRLPMQGEVELLCGGPPCQGFSGMNRFNSREYSNFKNSLVASYLSFCDYYRPKYFILENVRNFVAFKKGMVLKLTLRALLDMGYQCTFGILQAGNYGVPQTRRRLIILAAAPGYNLPFYPEPTHVFSRRACTLTTTIDGKRFSTNIHWDESAPKRTCTIQDAMSDLPQICNGANKIEIEYGSMPESHFQRLVRSNDENSKLRDHICKNMAPLIQARISRIPTTPGSDWRDLPNISVTLSDGTKCKVLQYRYDDKRNGRSSSGAVRGVCACASGRACSPLDKQENTLIPWCLPHTGNRHNHWAGLYGRLSWGGYFSTTVTDPEPMGKQGRVLHPDQHRVVSVRECARSQGFPDTYLFAGSVQDKHRQIGNAVPPPLGAALGREIKKALTLSLTTS

Target ORF information:

RefSeq Version	NM_001043515.2
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori DNA cytosine-5 methyltransferase (Dnmt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043515.2

ORF Insert Sequence:

ATGCCCACTT CAACTATTAC TTGCAACAGT ATAACACAAG TGTGCATGGA TAAGATACTT 
GATGGGGATG AAATAATCAA AGAAAACTCT AAGAGAAAAA GGAGCTGTGA AGAAATTGTT 
TCTGCCAATA ATAAACGATC AAAAACAGAA GACAATGATG GACATCAAAT TTCACAAGAT 
TCTACAAGCA ATGACTCTAT TGAAAATCTT AACATTCCAG CAAAAAATAC TAGTCTTATT 
GTTTCTGAAA ACATCAATAA TGTATATAAA AATTATGACG AGGTTGAAAA TAAACCAGTA 
TATAAAAATG GTGACCCTAT CGTTGTTGAA ATAGATAACA AGGATAAGCC TGGATCAGTT 
AACAACAATC ATGACATGAT TGATGATTCA GAGACCATAT CTAATATTAA GAATAGCAAT 
AATATTATCC CTGATACAGA GAAATGTAAC ATATGTGGCC AATTTTTAAA TAATTCTGAC 
TTAATTTATT ACCAAGGTCA CCCACAAGAT GCTGTTGAAG AATATATTGC ATTGACTAAT 
GACAAACTTG TTTTGAGCTC TGGGGAAGAT GGAGACATAA TGGAACGACC CCAAACAAAT 
ATAACTGGCT TTACAATATT TGATGAGCAA GGACATTTGT GTCCAATTGA TGGTGGTTTG 
GTAGAAAACG ATGTTCGAAT TTATATGTCT GGATATTTAA AGTCAATTTG CTCTGATTCA 
TCAGAGATTG ACGAAGAATC CATTCCAGTT AAAGATGTAG GTCCTATAAT AGAATGGTTC 
ATACATGGTT TCGATGGTGG AGATAGGAAC TGTATAACTT TATCAACGGA ATTTGGGGAA 
TATAACTTGC TCAAACCTAG TGAAGCTTAT ACGCCCCTAA TGAATAATTT ATATGAAAAA 
ATATGGCTTA GCAAAGTAGT CGTCGAATTC TTAGAAGAAT ATCACTACTT GCAACCGAGC 
TATGAAGATT TACTTGAAGT TGTAAGAGAT TTTTCCATTC CTGAACTAAA TAATAAAAAG 
ATGACAGAAG AAATGCTACA TAAACATGCC CAGTTTGTTT GTGATCAAGT AGTTAGCTTA 
GAAATAGAGG AAGATGATGA ACCATTAATA ACATTACCTT GTATGAGAGA ACTTATCAAA 
TTAATGGGCA TAAAATTCGG TAAACGAAAA ATACGTACCC AGATACAATA CAAAAAAACA 
GATAAGAAAG CATGGACAAA AGCTACAACA ACTCCACTTG TAAGAAAAAC ATTTGAAAGT 
TTTTTCTCAA ATCAGTTAGA CAAAACCAAT CACGAACTGG TCTTACGAAG AAAACGATGC 
GGGGTTTGTG AAGCCTGTCA ATTGCCTGAT TGCGGCGAAT GTAATGCATG TCGTGCCATG 
GCTAAATTCG GTGGTCATGG AAGAACTAAG AAAGCATGCG TCCGTAGATT ATGTCCTAAT 
ATGGCAGTAG AACAGGCAGA AGATTCTGAT CCCGATGATG AAGATGAGTA TCAGCAAATA 
TCTGAGAAAA AACAAGACAA AATAGATGAT GCAGTACCAG TCAAGCTCAC TGGGTCAAAT 
AGTAAAAATT TAAAATGGAT TGGTGAGCCT GTTAAAGCTG ATGCCACAAA AATATATTAC 
GAAAAAGTTG AAATTGATGG TGCTGAGCTG TGTAATGGTG ACTTTGTCAT GATTGAAACT 
TCCCAAACAA ACATTCCTAC ACTGGTCGCT AAAGTTGTAT ACATGTGGAA AGAAATCCAT 
AACCCGAAAT CAGGCTATTT TCACGGAGAA GTCTTCATAC GAGCCTCGGA CACAGTTTTA 
GGAGAAGTGA GTGACCCAAG AGAAGTATTT TTGGGCGACA GATGTTGCCA TGGTGCTCCG 
CTTTCTTCCA TATTAAGAAA AGCGAATATA GAAAGGAAAG AGACGTCAGC AGATTGGTTT 
AAACTTGGCG GGAAAGAAGT AGACGACGAA CATTTCGAGG ATGATGGAAG AACTTATTTT 
TATAGCAAGT ATTACGACAG GTTCACGTCG CGTTTTGAAG ATTTACCTCC AGATCCAGCG 
TGTCCAAATG CTTTAAGAAA GCACAGATTC TGCCCATCTT GTGAACGTAA AACAAAACGA 
GATGCAAGAA ACATTCCTAA AGTTTTTGAA AAATTGATTG TGAAATCCGA AATTGTTTCA 
GAACAAAACA GGTCGGAATG GTCATATGTA AAATGGCAAG ACTTTGACTA TAAGAAGGGT 
TGTGGTGTTT TCCTAAAACC GGGAACCTTT AAACTAAAAA ATAGTATGAC AAAAGCGAAC 
ACTGTTGCCA AACCGCGGTT TGAAAAAGTC GATGAAACTA TTTATCCTGA ATATTATAGG 
AAGAACGATT CTAATACGCG TGGGTCTAAT ATTGATACCG GGGAACCTTT TTGTGTGGGT 
TACATAGCTG CCGTCACTGC CGCGAGTGAG GGACCTCTAG TTGTACCGCA AGATATTTAC 
CTGAAAGTGA ATGTGCTGCT TAGACCTGAA AACACGAGCA GTAAATTTCC GCAGCACGAG 
GACACTAATG TTTTATATTG GACAACTGAA ATAAGGGAGA TTCCATTTTC GACCGTCGTT 
GGGCATTGTC ATTTAATTTA TGAACAAAAT GTTCCCCAAA ATATTTCACT TCAAGAATGG 
TTAGGAAATG ATCCGTGCAG ATTCTACTTC AGAATGGCGT ATTGTAAGTC CACGGGAGAG 
TTCACGGATC TCCCGCAGAA TGCCATCAGT GTTGGAAGAA CAGATCGTAC TAAAGATAAA 
GGGAAGGGGA AGGGAAAATC TACAAAAACT ATTGAAACGG TTCCCGCTAA AGTTGTAGAG 
GAGAAAATTA GACCGTTGCG AACTTTAGAC GTGTTTGCCG GTTGTGGGGG CCTATCAGAA 
GGCTTGCATC AAGCTGGAGT TGCAGAATGC AAGTGGGCTA TTGAAAACGT GGAAGCTGCT 
TCTCATGCTT ATTCATTAAA TAATAAAAGT TGTATTGTAT TTAATGAAGA CTGTAATGCA 
CTTTTGAAAA CTGTAATGTC TGGTGCTAAG CACAGCGCGA ATGGACTGCG GCTCCCCATG 
CAAGGAGAAG TGGAGCTTTT ATGTGGCGGG CCACCATGTC AAGGCTTCTC CGGAATGAAT 
CGTTTTAATT CAAGAGAGTA TTCAAACTTC AAAAACTCAT TAGTTGCATC GTATTTATCG 
TTTTGTGATT ATTACAGACC TAAATATTTT ATACTGGAAA ACGTTCGTAA CTTTGTGGCC 
TTTAAAAAAG GTATGGTTTT GAAACTCACT CTGCGAGCTT TGTTGGACAT GGGATACCAG 
TGTACCTTTG GTATCTTACA AGCGGGTAAC TACGGAGTTC CCCAAACTAG ACGAAGGTTG 
ATCATATTGG CGGCAGCGCC GGGTTACAAT CTTCCGTTCT ACCCAGAACC CACGCACGTC 
TTCAGTAGAC GTGCTTGTAC ATTAACAACG ACGATTGACG GCAAAAGGTT CTCGACTAAT 
ATTCACTGGG ACGAGTCGGC TCCAAAAAGA ACGTGCACCA TTCAAGACGC CATGAGTGAT 
TTGCCTCAAA TATGTAATGG TGCTAATAAA ATAGAAATCG AATATGGCTC CATGCCAGAA 
AGTCATTTCC AGAGATTAGT GCGAAGCAAC GATGAGAATT CTAAACTACG AGATCACATC 
TGTAAAAACA TGGCGCCATT GATTCAAGCT AGGATCAGCA GGATACCTAC AACACCGGGC 
TCCGACTGGA GAGATCTACC CAACATATCC GTTACTCTCT CCGATGGCAC TAAGTGCAAG 
GTTCTACAGT ATCGCTACGA CGACAAGCGC AATGGGCGGT CGAGCAGCGG CGCAGTGCGC 
GGCGTGTGCG CGTGCGCGAG CGGCCGCGCC TGCTCGCCGC TGGACAAGCA GGAGAACACG 
CTCATCCCGT GGTGTCTGCC GCACACCGGC AACAGGCACA ACCACTGGGC CGGGCTCTAC 
GGTCGGCTAT CGTGGGGCGG GTACTTCAGC ACGACAGTGA CGGATCCCGA ACCGATGGGC 
AAGCAAGGCC GGGTGCTCCA CCCCGACCAG CACCGAGTCG TCTCCGTGCG AGAGTGCGCG 
CGCTCGCAGG GCTTCCCTGA TACGTATCTC TTCGCAGGCT CCGTACAGGA CAAACATCGA 
CAGATCGGAA ATGCAGTTCC ACCACCTCTT GGCGCCGCTC TGGGTAGAGA AATAAAGAAA 
GCGCTTACTC TCAGCCTGAC CACATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnmt1 ( XM_012695406.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_012550860.1 Bombyx mori DNA cytosine-5 methyltransferase (Dnmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBg13919
Clone ID Related Accession (Same CDS sequence)	XM_012695406.1 , XM_012695406.2
Accession Version	XM_012695406.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433347200000
Organism	Bombyx mori(Domestic silkworm)
Product	DNA cytosine-5 methyltransferase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582026.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTGCA TGGATAAGAT ACTTGATGGG GATGAAATAA TCAAAGAAAA CTCTAAGAGA 
AAAAGGAGCT GTGAAGAAAT TGTTTCTGCC AATAATAAAC GATCAAAAAC AGAAGACAAT 
GATGGACATC AAATTTCACA AGATTCTACA AGCAATGACT CTATTGAAAA TCTTAACATT 
CCAGCAAAAA ATACTAGTCT TATTGTTTCT GAAAACATCA ATAATGTATA TAAAAATTAT 
GACGAGGTTG AAAATAAACC AGTATATAAA AATGGTGACC CTATCGTTGT TGAAATAGAT 
AACAAGGATA AGCCTGGATC AGTTAACAAC AATCATGACA TGATTGATGA TTCAGAGACC 
ATATCTAATA TTAAGAATAG CAATAATATT ATCCCTGATA CAGAGAAATG TAACATATGT 
GGCCAATTTT TAAATAATTC TGACTTAATT TATTACCAAG GTCACCCACA AGATGCTGTT 
GAAGAATATA TTGCATTGAC TAATGACAAA CTTGTTTTGA GCTCTGGGGA AGATGGAGAC 
ATAATGGAAC GACCCCAAAC AAATATAACT GGCTTTACAA TATTTGATGA GCAAGGACAT 
TTGTGTCCAA TTGATGGTGG TTTGGTAGAA AACGATGTTC GAATTTATAT GTCTGGATAT 
TTAAAGTCAA TTTGCTCTGA TTCATCAGAG ATTGACGAAG AATCCATTCC AGTTAAAGAT 
GTAGGTCCTA TAATAGAATG GTTCATACAT GGTTTCGATG GTGGAGATAG GAACTGTATA 
ACTTTATCAA CGGAATTTGG GGAATATAAC TTGCTCAAAC CTAGTGAAGC TTATACGCCC 
CTAATGAATA ATTTATATGA AAAAATATGG CTTAGCAAAG TAGTCGTCGA ATTCTTAGAA 
GAATATCACT ACTTGCAACC GAGCTATGAA GATTTACTTG AAGTTGTAAG AGATTTTTCC 
ATTCCTGAAC TAAATAATAA AAAGATGACA GAAGAAATGC TACATAAACA TGCCCAGTTT 
GTTTGTGATC AAGTAGTTAG CTTAGAAATA GAGGAAGATG ATGAACCATT AATAACATTA 
CCTTGTATGA GAGAACTTAT CAAATTAATG GGCATAAAAT TCGGTAAACG AAAAATACGT 
ACCCAGATAC AATACAAAAA AACAGATAAG AAAGCATGGA CAAAAGCTAC AACAACTCCA 
CTTGTAAGAA AAACATTTGA AAGTTTTTTC TCAAATCAGT TAGACAAAAC CAATCACGAA 
CTGGTCTTAC GAAGAAAACG ATGCGGGGTT TGTGAAGCCT GTCAATTGCC TGATTGCGGC 
GAATGTAATG CATGTCGTGC CATGGCTAAA TTCGGTGGTC ATGGAAGAAC TAAGAAAGCA 
TGCGTCCGTA GATTATGTCC TAATATGGCA GTAGAACAGG CAGAAGATTC TGATCCCGAT 
GATGAAGATG AGTATCAGCA AATATCTGAG AAAAAACAAG ACAAAATAGA TGATGCAGTA 
CCAGTCAAGC TCACTGGGTC AAATAGTAAA AATTTAAAAT GGATTGGTGA GCCTGTTAAA 
GCTGATGCCA CAAAAATATA TTACGAAAAA GTTGAAATTG ATGGTGCTGA GCTGTGTAAT 
GGTGACTTTG TCATGATTGA AACTTCCCAA ACAAACATTC CTACACTGGT CGCTAAAGTT 
GTATACATGT GGAAAGAAAT CCATAACCCG AAATCAGGCT ATTTTCACGG AGAAGTCTTC 
ATACGAGCCT CGGACACAGT TTTAGGAGAA GTGAGTGACC CAAGAGAAGT ATTTTTGGGC 
GACAGATGTT GCCATGGTGC TCCGCTTTCT TCCATATTAA GAAAAGCGAA TATAGAAAGG 
AAAGAGACGT CAGCAGATTG GTTTAAACTT GGCGGGAAAG AAGTAGACGA CGAACATTTC 
GAGGATGATG GAAGAACTTA TTTTTATAGC AAGTATTACG ACAGGTTCAC GTCGCGTTTT 
GAAGATTTAC CTCCAGATCC AGCGTGTCCA AATGCTTTAA GAAAGCACAG ATTCTGCCCA 
TCTTGTGAAC GTAAAACAAA ACGAGATGCA AGAAACATTC CTAAAGTTTT TGAAAAATTG 
ATTGTGAAAT CCGAAATTGT TTCAGAACAA AACAGGTCGG AATGGTCATA TGTAAAATGG 
CAAGACTTTG ACTATAAGAA GGGTTGTGGT GTTTTCCTAA AACCGGGAAC CTTTAAACTA 
AAAAATAGTA TGACAAAAGC GAACACTGTT GCCAAACCGC GGTTTGAAAA AGTCGATGAA 
ACTATTTATC CTGAATATTA TAGGAAGAAC GATTCTAATA CGCGTGGGTC TAATATTGAT 
ACCGGGGAAC CTTTTTGTGT GGGTTACATA GCTGCCGTCA CTGCCGCGAG TGAGGGACCT 
CTAGTTGTAC CGCAAGATAT TTACCTGAAA GTGAATGTGC TGCTTAGACC TGAAAACACG 
AGCAGTAAAT TTCCGCAGCA CGAGGACACT AATGTTTTAT ATTGGACAAC TGAAATAAGG 
GAGATTCCAT TTTCGACCGT CGTTGGGCAT TGTCATTTAA TTTATGAACA AAATGTTCCC 
CAAAATATTT CACTTCAAGA ATGGTTAGGA AATGATCCGT GCAGATTCTA CTTCAGAATG 
GCGTATTGTA AGTCCACGGG AGAGTTCACG GATCTCCCGC AGAATGCCAT CAGTGTTGGA 
AGAACAGATC GTACTAAAGA TAAAGGGAAG GGGAAGGGAA AATCTACAAA AACTATTGAA 
ACGGTTCCCG CTAAAGTTGT AGAGGAGAAA ATTAGACCGT TGCGAACTTT AGACGTGTTT 
GCCGGTTGTG GGGGCCTATC AGAAGGCTTG CATCAAGCTG GAGTTGCAGA ATGCAAGTGG 
GCTATTGAAA ACGTGGAAGC TGCTTCTCAT GCTTATTCAT TAAATAATAA AAGTTGTATT 
GTATTTAATG AAGACTGTAA TGCACTTTTG AAAACTGTAA TGTCTGGTGC TAAGCACAGC 
GCGAATGGAC TGCGGCTCCC CATGCAAGGA GAAGTGGAGC TTTTATGTGG CGGGCCACCA 
TGTCAAGGCT TCTCCGGAAT GAATCGTTTT AATTCAAGAG AGTATTCAAA CTTCAAAAAC 
TCATTAGTTG CATCGTATTT ATCGTTTTGT GATTATTACA GACCTAAATA TTTTATACTG 
GAAAACGTTC GTAACTTTGT GGCCTTTAAA AAAGGTATGG TTTTGAAACT CACTCTGCGA 
GCTTTGTTGG ACATGGGATA CCAGTGTACC TTTGGTATCT TACAAGCGGG TAACTACGGA 
GTTCCCCAAA CTAGACGAAG GTTGATCATA TTGGCGGCAG CGCCGGGTTA CAATCTTCCG 
TTCTACCCAG AACCCACGCA CGTCTTCAGT AGACGTGCTT GTACATTAAC AACGACGATT 
GACGGCAAAA GGTTCTCGAC TAATATTCAC TGGGACGAGT CGGCTCCAAA AAGAACGTGC 
ACCATTCAAG ACGCCATGAG TGATTTGCCT CAAATATGTA ATGGTGCTAA TAAAATAGAA 
ATCGAATATG GCTCCATGCC AGAAAGTCAT TTCCAGAGAT TAGTGCGAAG CAACGATGAG 
AATTCTAAAC TACGAGATCA CATCTGTAAA AACATGGCGC CATTGATTCA AGCTAGGATC 
AGCAGGATAC CTACAACACC GGGCTCCGAC TGGAGAGATC TACCCAACAT ATCGGTTACT 
CTCTCCGATG GCACTAAGTG CAAGGTTCTA CAGTATCGCT ACGACGACAA GCGCAATGGG 
CGGTCGAGCA GCGGCGCAGT GCGCGGCGTG TGCGCGTGCG CGAGCGGCCG CGCCTGCTCG 
CCGCTGGACA AGCAGGAGAA CACGCTCATC CCGTGGTGTC TGCCGCACAC CGGCAACAGG 
CACAACCACT GGGCCGGGCT CTACGGTCGG CTATCGTGGG GCGGGTACTT CAGCACGACA 
GTGACGGATC CCGAACCGAT GGGCAAGCAA GGCCGGGTGC TCCACCCCGA CCAGCACCGA 
GTCGTCTCCG TGCGAGAGTG CGCGCGCTCG CAGGGCTTCC CTGATACGTA TCTCTTCGCA 
GGCTCCGTAC AGGACAAACA TCGACAGATC GGAAATGCAG TTCCACCACC TCTTGGCGCC 
GCTCTGGGTA GAGAAATAAA GAAAGCGCTT ACTCTCAGCC TGACCACATC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012550860.1
CDS	199..4392
Translation	MRCMDKILDGDEIIKENSKRKRSCEEIVSANNKRSKTEDNDGHQISQDSTSNDSIENLNIPAKNTSLIVSENINNVYKNYDEVENKPVYKNGDPIVVEIDNKDKPGSVNNNHDMIDDSETISNIKNSNNIIPDTEKCNICGQFLNNSDLIYYQGHPQDAVEEYIALTNDKLVLSSGEDGDIMERPQTNITGFTIFDEQGHLCPIDGGLVENDVRIYMSGYLKSICSDSSEIDEESIPVKDVGPIIEWFIHGFDGGDRNCITLSTEFGEYNLLKPSEAYTPLMNNLYEKIWLSKVVVEFLEEYHYLQPSYEDLLEVVRDFSIPELNNKKMTEEMLHKHAQFVCDQVVSLEIEEDDEPLITLPCMRELIKLMGIKFGKRKIRTQIQYKKTDKKAWTKATTTPLVRKTFESFFSNQLDKTNHELVLRRKRCGVCEACQLPDCGECNACRAMAKFGGHGRTKKACVRRLCPNMAVEQAEDSDPDDEDEYQQISEKKQDKIDDAVPVKLTGSNSKNLKWIGEPVKADATKIYYEKVEIDGAELCNGDFVMIETSQTNIPTLVAKVVYMWKEIHNPKSGYFHGEVFIRASDTVLGEVSDPREVFLGDRCCHGAPLSSILRKANIERKETSADWFKLGGKEVDDEHFEDDGRTYFYSKYYDRFTSRFEDLPPDPACPNALRKHRFCPSCERKTKRDARNIPKVFEKLIVKSEIVSEQNRSEWSYVKWQDFDYKKGCGVFLKPGTFKLKNSMTKANTVAKPRFEKVDETIYPEYYRKNDSNTRGSNIDTGEPFCVGYIAAVTAASEGPLVVPQDIYLKVNVLLRPENTSSKFPQHEDTNVLYWTTEIREIPFSTVVGHCHLIYEQNVPQNISLQEWLGNDPCRFYFRMAYCKSTGEFTDLPQNAISVGRTDRTKDKGKGKGKSTKTIETVPAKVVEEKIRPLRTLDVFAGCGGLSEGLHQAGVAECKWAIENVEAASHAYSLNNKSCIVFNEDCNALLKTVMSGAKHSANGLRLPMQGEVELLCGGPPCQGFSGMNRFNSREYSNFKNSLVASYLSFCDYYRPKYFILENVRNFVAFKKGMVLKLTLRALLDMGYQCTFGILQAGNYGVPQTRRRLIILAAAPGYNLPFYPEPTHVFSRRACTLTTTIDGKRFSTNIHWDESAPKRTCTIQDAMSDLPQICNGANKIEIEYGSMPESHFQRLVRSNDENSKLRDHICKNMAPLIQARISRIPTTPGSDWRDLPNISVTLSDGTKCKVLQYRYDDKRNGRSSSGAVRGVCACASGRACSPLDKQENTLIPWCLPHTGNRHNHWAGLYGRLSWGGYFSTTVTDPEPMGKQGRVLHPDQHRVVSVRECARSQGFPDTYLFAGSVQDKHRQIGNAVPPPLGAALGREIKKALTLSLTTS

Target ORF information:

RefSeq Version	XM_012695406.1
Organism	Bombyx mori(Domestic silkworm)
Definition	Bombyx mori DNA cytosine-5 methyltransferase (Dnmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012695406.1

ORF Insert Sequence:

ATGAGGTGCA TGGATAAGAT ACTTGATGGG GATGAAATAA TCAAAGAAAA CTCTAAGAGA 
AAAAGGAGCT GTGAAGAAAT TGTTTCTGCC AATAATAAAC GATCAAAAAC AGAAGACAAT 
GATGGACATC AAATTTCACA AGATTCTACA AGCAATGACT CTATTGAAAA TCTTAACATT 
CCAGCAAAAA ATACTAGTCT TATTGTTTCT GAAAACATCA ATAATGTATA TAAAAATTAT 
GACGAGGTTG AAAATAAACC AGTATATAAA AATGGTGACC CTATCGTTGT TGAAATAGAT 
AACAAGGATA AGCCTGGATC AGTTAACAAC AATCATGACA TGATTGATGA TTCAGAGACC 
ATATCTAATA TTAAGAATAG CAATAATATT ATCCCTGATA CAGAGAAATG TAACATATGT 
GGCCAATTTT TAAATAATTC TGACTTAATT TATTACCAAG GTCACCCACA AGATGCTGTT 
GAAGAATATA TTGCATTGAC TAATGACAAA CTTGTTTTGA GCTCTGGGGA AGATGGAGAC 
ATAATGGAAC GACCCCAAAC AAATATAACT GGCTTTACAA TATTTGATGA GCAAGGACAT 
TTGTGTCCAA TTGATGGTGG TTTGGTAGAA AACGATGTTC GAATTTATAT GTCTGGATAT 
TTAAAGTCAA TTTGCTCTGA TTCATCAGAG ATTGACGAAG AATCCATTCC AGTTAAAGAT 
GTAGGTCCTA TAATAGAATG GTTCATACAT GGTTTCGATG GTGGAGATAG GAACTGTATA 
ACTTTATCAA CGGAATTTGG GGAATATAAC TTGCTCAAAC CTAGTGAAGC TTATACGCCC 
CTAATGAATA ATTTATATGA AAAAATATGG CTTAGCAAAG TAGTCGTCGA ATTCTTAGAA 
GAATATCACT ACTTGCAACC GAGCTATGAA GATTTACTTG AAGTTGTAAG AGATTTTTCC 
ATTCCTGAAC TAAATAATAA AAAGATGACA GAAGAAATGC TACATAAACA TGCCCAGTTT 
GTTTGTGATC AAGTAGTTAG CTTAGAAATA GAGGAAGATG ATGAACCATT AATAACATTA 
CCTTGTATGA GAGAACTTAT CAAATTAATG GGCATAAAAT TCGGTAAACG AAAAATACGT 
ACCCAGATAC AATACAAAAA AACAGATAAG AAAGCATGGA CAAAAGCTAC AACAACTCCA 
CTTGTAAGAA AAACATTTGA AAGTTTTTTC TCAAATCAGT TAGACAAAAC CAATCACGAA 
CTGGTCTTAC GAAGAAAACG ATGCGGGGTT TGTGAAGCCT GTCAATTGCC TGATTGCGGC 
GAATGTAATG CATGTCGTGC CATGGCTAAA TTCGGTGGTC ATGGAAGAAC TAAGAAAGCA 
TGCGTCCGTA GATTATGTCC TAATATGGCA GTAGAACAGG CAGAAGATTC TGATCCCGAT 
GATGAAGATG AGTATCAGCA AATATCTGAG AAAAAACAAG ACAAAATAGA TGATGCAGTA 
CCAGTCAAGC TCACTGGGTC AAATAGTAAA AATTTAAAAT GGATTGGTGA GCCTGTTAAA 
GCTGATGCCA CAAAAATATA TTACGAAAAA GTTGAAATTG ATGGTGCTGA GCTGTGTAAT 
GGTGACTTTG TCATGATTGA AACTTCCCAA ACAAACATTC CTACACTGGT CGCTAAAGTT 
GTATACATGT GGAAAGAAAT CCATAACCCG AAATCAGGCT ATTTTCACGG AGAAGTCTTC 
ATACGAGCCT CGGACACAGT TTTAGGAGAA GTGAGTGACC CAAGAGAAGT ATTTTTGGGC 
GACAGATGTT GCCATGGTGC TCCGCTTTCT TCCATATTAA GAAAAGCGAA TATAGAAAGG 
AAAGAGACGT CAGCAGATTG GTTTAAACTT GGCGGGAAAG AAGTAGACGA CGAACATTTC 
GAGGATGATG GAAGAACTTA TTTTTATAGC AAGTATTACG ACAGGTTCAC GTCGCGTTTT 
GAAGATTTAC CTCCAGATCC AGCGTGTCCA AATGCTTTAA GAAAGCACAG ATTCTGCCCA 
TCTTGTGAAC GTAAAACAAA ACGAGATGCA AGAAACATTC CTAAAGTTTT TGAAAAATTG 
ATTGTGAAAT CCGAAATTGT TTCAGAACAA AACAGGTCGG AATGGTCATA TGTAAAATGG 
CAAGACTTTG ACTATAAGAA GGGTTGTGGT GTTTTCCTAA AACCGGGAAC CTTTAAACTA 
AAAAATAGTA TGACAAAAGC GAACACTGTT GCCAAACCGC GGTTTGAAAA AGTCGATGAA 
ACTATTTATC CTGAATATTA TAGGAAGAAC GATTCTAATA CGCGTGGGTC TAATATTGAT 
ACCGGGGAAC CTTTTTGTGT GGGTTACATA GCTGCCGTCA CTGCCGCGAG TGAGGGACCT 
CTAGTTGTAC CGCAAGATAT TTACCTGAAA GTGAATGTGC TGCTTAGACC TGAAAACACG 
AGCAGTAAAT TTCCGCAGCA CGAGGACACT AATGTTTTAT ATTGGACAAC TGAAATAAGG 
GAGATTCCAT TTTCGACCGT CGTTGGGCAT TGTCATTTAA TTTATGAACA AAATGTTCCC 
CAAAATATTT CACTTCAAGA ATGGTTAGGA AATGATCCGT GCAGATTCTA CTTCAGAATG 
GCGTATTGTA AGTCCACGGG AGAGTTCACG GATCTCCCGC AGAATGCCAT CAGTGTTGGA 
AGAACAGATC GTACTAAAGA TAAAGGGAAG GGGAAGGGAA AATCTACAAA AACTATTGAA 
ACGGTTCCCG CTAAAGTTGT AGAGGAGAAA ATTAGACCGT TGCGAACTTT AGACGTGTTT 
GCCGGTTGTG GGGGCCTATC AGAAGGCTTG CATCAAGCTG GAGTTGCAGA ATGCAAGTGG 
GCTATTGAAA ACGTGGAAGC TGCTTCTCAT GCTTATTCAT TAAATAATAA AAGTTGTATT 
GTATTTAATG AAGACTGTAA TGCACTTTTG AAAACTGTAA TGTCTGGTGC TAAGCACAGC 
GCGAATGGAC TGCGGCTCCC CATGCAAGGA GAAGTGGAGC TTTTATGTGG CGGGCCACCA 
TGTCAAGGCT TCTCCGGAAT GAATCGTTTT AATTCAAGAG AGTATTCAAA CTTCAAAAAC 
TCATTAGTTG CATCGTATTT ATCGTTTTGT GATTATTACA GACCTAAATA TTTTATACTG 
GAAAACGTTC GTAACTTTGT GGCCTTTAAA AAAGGTATGG TTTTGAAACT CACTCTGCGA 
GCTTTGTTGG ACATGGGATA CCAGTGTACC TTTGGTATCT TACAAGCGGG TAACTACGGA 
GTTCCCCAAA CTAGACGAAG GTTGATCATA TTGGCGGCAG CGCCGGGTTA CAATCTTCCG 
TTCTACCCAG AACCCACGCA CGTCTTCAGT AGACGTGCTT GTACATTAAC AACGACGATT 
GACGGCAAAA GGTTCTCGAC TAATATTCAC TGGGACGAGT CGGCTCCAAA AAGAACGTGC 
ACCATTCAAG ACGCCATGAG TGATTTGCCT CAAATATGTA ATGGTGCTAA TAAAATAGAA 
ATCGAATATG GCTCCATGCC AGAAAGTCAT TTCCAGAGAT TAGTGCGAAG CAACGATGAG 
AATTCTAAAC TACGAGATCA CATCTGTAAA AACATGGCGC CATTGATTCA AGCTAGGATC 
AGCAGGATAC CTACAACACC GGGCTCCGAC TGGAGAGATC TACCCAACAT ATCGGTTACT 
CTCTCCGATG GCACTAAGTG CAAGGTTCTA CAGTATCGCT ACGACGACAA GCGCAATGGG 
CGGTCGAGCA GCGGCGCAGT GCGCGGCGTG TGCGCGTGCG CGAGCGGCCG CGCCTGCTCG 
CCGCTGGACA AGCAGGAGAA CACGCTCATC CCGTGGTGTC TGCCGCACAC CGGCAACAGG 
CACAACCACT GGGCCGGGCT CTACGGTCGG CTATCGTGGG GCGGGTACTT CAGCACGACA 
GTGACGGATC CCGAACCGAT GGGCAAGCAA GGCCGGGTGC TCCACCCCGA CCAGCACCGA 
GTCGTCTCCG TGCGAGAGTG CGCGCGCTCG CAGGGCTTCC CTGATACGTA TCTCTTCGCA 
GGCTCCGTAC AGGACAAACA TCGACAGATC GGAAATGCAG TTCCACCACC TCTTGGCGCC 
GCTCTGGGTA GAGAAATAAA GAAAGCGCTT ACTCTCAGCC TGACCACATC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnmt1 ( XM_012695406.2 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_012550860.1 Bombyx mori DNA cytosine-5 methyltransferase (Dnmt1), transcript variant X1, mRNA.

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CloneID	OBg13919
Clone ID Related Accession (Same CDS sequence)	XM_012695406.1 , XM_012695406.2
Accession Version	XM_012695406.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	DNA cytosine-5 methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582026.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 5, 2017 this sequence version replaced XM_012695406.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTGCA TGGATAAGAT ACTTGATGGG GATGAAATAA TCAAAGAAAA CTCTAAGAGA 
AAAAGGAGCT GTGAAGAAAT TGTTTCTGCC AATAATAAAC GATCAAAAAC AGAAGACAAT 
GATGGACATC AAATTTCACA AGATTCTACA AGCAATGACT CTATTGAAAA TCTTAACATT 
CCAGCAAAAA ATACTAGTCT TATTGTTTCT GAAAACATCA ATAATGTATA TAAAAATTAT 
GACGAGGTTG AAAATAAACC AGTATATAAA AATGGTGACC CTATCGTTGT TGAAATAGAT 
AACAAGGATA AGCCTGGATC AGTTAACAAC AATCATGACA TGATTGATGA TTCAGAGACC 
ATATCTAATA TTAAGAATAG CAATAATATT ATCCCTGATA CAGAGAAATG TAACATATGT 
GGCCAATTTT TAAATAATTC TGACTTAATT TATTACCAAG GTCACCCACA AGATGCTGTT 
GAAGAATATA TTGCATTGAC TAATGACAAA CTTGTTTTGA GCTCTGGGGA AGATGGAGAC 
ATAATGGAAC GACCCCAAAC AAATATAACT GGCTTTACAA TATTTGATGA GCAAGGACAT 
TTGTGTCCAA TTGATGGTGG TTTGGTAGAA AACGATGTTC GAATTTATAT GTCTGGATAT 
TTAAAGTCAA TTTGCTCTGA TTCATCAGAG ATTGACGAAG AATCCATTCC AGTTAAAGAT 
GTAGGTCCTA TAATAGAATG GTTCATACAT GGTTTCGATG GTGGAGATAG GAACTGTATA 
ACTTTATCAA CGGAATTTGG GGAATATAAC TTGCTCAAAC CTAGTGAAGC TTATACGCCC 
CTAATGAATA ATTTATATGA AAAAATATGG CTTAGCAAAG TAGTCGTCGA ATTCTTAGAA 
GAATATCACT ACTTGCAACC GAGCTATGAA GATTTACTTG AAGTTGTAAG AGATTTTTCC 
ATTCCTGAAC TAAATAATAA AAAGATGACA GAAGAAATGC TACATAAACA TGCCCAGTTT 
GTTTGTGATC AAGTAGTTAG CTTAGAAATA GAGGAAGATG ATGAACCATT AATAACATTA 
CCTTGTATGA GAGAACTTAT CAAATTAATG GGCATAAAAT TCGGTAAACG AAAAATACGT 
ACCCAGATAC AATACAAAAA AACAGATAAG AAAGCATGGA CAAAAGCTAC AACAACTCCA 
CTTGTAAGAA AAACATTTGA AAGTTTTTTC TCAAATCAGT TAGACAAAAC CAATCACGAA 
CTGGTCTTAC GAAGAAAACG ATGCGGGGTT TGTGAAGCCT GTCAATTGCC TGATTGCGGC 
GAATGTAATG CATGTCGTGC CATGGCTAAA TTCGGTGGTC ATGGAAGAAC TAAGAAAGCA 
TGCGTCCGTA GATTATGTCC TAATATGGCA GTAGAACAGG CAGAAGATTC TGATCCCGAT 
GATGAAGATG AGTATCAGCA AATATCTGAG AAAAAACAAG ACAAAATAGA TGATGCAGTA 
CCAGTCAAGC TCACTGGGTC AAATAGTAAA AATTTAAAAT GGATTGGTGA GCCTGTTAAA 
GCTGATGCCA CAAAAATATA TTACGAAAAA GTTGAAATTG ATGGTGCTGA GCTGTGTAAT 
GGTGACTTTG TCATGATTGA AACTTCCCAA ACAAACATTC CTACACTGGT CGCTAAAGTT 
GTATACATGT GGAAAGAAAT CCATAACCCG AAATCAGGCT ATTTTCACGG AGAAGTCTTC 
ATACGAGCCT CGGACACAGT TTTAGGAGAA GTGAGTGACC CAAGAGAAGT ATTTTTGGGC 
GACAGATGTT GCCATGGTGC TCCGCTTTCT TCCATATTAA GAAAAGCGAA TATAGAAAGG 
AAAGAGACGT CAGCAGATTG GTTTAAACTT GGCGGGAAAG AAGTAGACGA CGAACATTTC 
GAGGATGATG GAAGAACTTA TTTTTATAGC AAGTATTACG ACAGGTTCAC GTCGCGTTTT 
GAAGATTTAC CTCCAGATCC AGCGTGTCCA AATGCTTTAA GAAAGCACAG ATTCTGCCCA 
TCTTGTGAAC GTAAAACAAA ACGAGATGCA AGAAACATTC CTAAAGTTTT TGAAAAATTG 
ATTGTGAAAT CCGAAATTGT TTCAGAACAA AACAGGTCGG AATGGTCATA TGTAAAATGG 
CAAGACTTTG ACTATAAGAA GGGTTGTGGT GTTTTCCTAA AACCGGGAAC CTTTAAACTA 
AAAAATAGTA TGACAAAAGC GAACACTGTT GCCAAACCGC GGTTTGAAAA AGTCGATGAA 
ACTATTTATC CTGAATATTA TAGGAAGAAC GATTCTAATA CGCGTGGGTC TAATATTGAT 
ACCGGGGAAC CTTTTTGTGT GGGTTACATA GCTGCCGTCA CTGCCGCGAG TGAGGGACCT 
CTAGTTGTAC CGCAAGATAT TTACCTGAAA GTGAATGTGC TGCTTAGACC TGAAAACACG 
AGCAGTAAAT TTCCGCAGCA CGAGGACACT AATGTTTTAT ATTGGACAAC TGAAATAAGG 
GAGATTCCAT TTTCGACCGT CGTTGGGCAT TGTCATTTAA TTTATGAACA AAATGTTCCC 
CAAAATATTT CACTTCAAGA ATGGTTAGGA AATGATCCGT GCAGATTCTA CTTCAGAATG 
GCGTATTGTA AGTCCACGGG AGAGTTCACG GATCTCCCGC AGAATGCCAT CAGTGTTGGA 
AGAACAGATC GTACTAAAGA TAAAGGGAAG GGGAAGGGAA AATCTACAAA AACTATTGAA 
ACGGTTCCCG CTAAAGTTGT AGAGGAGAAA ATTAGACCGT TGCGAACTTT AGACGTGTTT 
GCCGGTTGTG GGGGCCTATC AGAAGGCTTG CATCAAGCTG GAGTTGCAGA ATGCAAGTGG 
GCTATTGAAA ACGTGGAAGC TGCTTCTCAT GCTTATTCAT TAAATAATAA AAGTTGTATT 
GTATTTAATG AAGACTGTAA TGCACTTTTG AAAACTGTAA TGTCTGGTGC TAAGCACAGC 
GCGAATGGAC TGCGGCTCCC CATGCAAGGA GAAGTGGAGC TTTTATGTGG CGGGCCACCA 
TGTCAAGGCT TCTCCGGAAT GAATCGTTTT AATTCAAGAG AGTATTCAAA CTTCAAAAAC 
TCATTAGTTG CATCGTATTT ATCGTTTTGT GATTATTACA GACCTAAATA TTTTATACTG 
GAAAACGTTC GTAACTTTGT GGCCTTTAAA AAAGGTATGG TTTTGAAACT CACTCTGCGA 
GCTTTGTTGG ACATGGGATA CCAGTGTACC TTTGGTATCT TACAAGCGGG TAACTACGGA 
GTTCCCCAAA CTAGACGAAG GTTGATCATA TTGGCGGCAG CGCCGGGTTA CAATCTTCCG 
TTCTACCCAG AACCCACGCA CGTCTTCAGT AGACGTGCTT GTACATTAAC AACGACGATT 
GACGGCAAAA GGTTCTCGAC TAATATTCAC TGGGACGAGT CGGCTCCAAA AAGAACGTGC 
ACCATTCAAG ACGCCATGAG TGATTTGCCT CAAATATGTA ATGGTGCTAA TAAAATAGAA 
ATCGAATATG GCTCCATGCC AGAAAGTCAT TTCCAGAGAT TAGTGCGAAG CAACGATGAG 
AATTCTAAAC TACGAGATCA CATCTGTAAA AACATGGCGC CATTGATTCA AGCTAGGATC 
AGCAGGATAC CTACAACACC GGGCTCCGAC TGGAGAGATC TACCCAACAT ATCGGTTACT 
CTCTCCGATG GCACTAAGTG CAAGGTTCTA CAGTATCGCT ACGACGACAA GCGCAATGGG 
CGGTCGAGCA GCGGCGCAGT GCGCGGCGTG TGCGCGTGCG CGAGCGGCCG CGCCTGCTCG 
CCGCTGGACA AGCAGGAGAA CACGCTCATC CCGTGGTGTC TGCCGCACAC CGGCAACAGG 
CACAACCACT GGGCCGGGCT CTACGGTCGG CTATCGTGGG GCGGGTACTT CAGCACGACA 
GTGACGGATC CCGAACCGAT GGGCAAGCAA GGCCGGGTGC TCCACCCCGA CCAGCACCGA 
GTCGTCTCCG TGCGAGAGTG CGCGCGCTCG CAGGGCTTCC CTGATACGTA TCTCTTCGCA 
GGCTCCGTAC AGGACAAACA TCGACAGATC GGAAATGCAG TTCCACCACC TCTTGGCGCC 
GCTCTGGGTA GAGAAATAAA GAAAGCGCTT ACTCTCAGCC TGACCACATC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012550860.1
CDS	175..4368
Translation	MRCMDKILDGDEIIKENSKRKRSCEEIVSANNKRSKTEDNDGHQISQDSTSNDSIENLNIPAKNTSLIVSENINNVYKNYDEVENKPVYKNGDPIVVEIDNKDKPGSVNNNHDMIDDSETISNIKNSNNIIPDTEKCNICGQFLNNSDLIYYQGHPQDAVEEYIALTNDKLVLSSGEDGDIMERPQTNITGFTIFDEQGHLCPIDGGLVENDVRIYMSGYLKSICSDSSEIDEESIPVKDVGPIIEWFIHGFDGGDRNCITLSTEFGEYNLLKPSEAYTPLMNNLYEKIWLSKVVVEFLEEYHYLQPSYEDLLEVVRDFSIPELNNKKMTEEMLHKHAQFVCDQVVSLEIEEDDEPLITLPCMRELIKLMGIKFGKRKIRTQIQYKKTDKKAWTKATTTPLVRKTFESFFSNQLDKTNHELVLRRKRCGVCEACQLPDCGECNACRAMAKFGGHGRTKKACVRRLCPNMAVEQAEDSDPDDEDEYQQISEKKQDKIDDAVPVKLTGSNSKNLKWIGEPVKADATKIYYEKVEIDGAELCNGDFVMIETSQTNIPTLVAKVVYMWKEIHNPKSGYFHGEVFIRASDTVLGEVSDPREVFLGDRCCHGAPLSSILRKANIERKETSADWFKLGGKEVDDEHFEDDGRTYFYSKYYDRFTSRFEDLPPDPACPNALRKHRFCPSCERKTKRDARNIPKVFEKLIVKSEIVSEQNRSEWSYVKWQDFDYKKGCGVFLKPGTFKLKNSMTKANTVAKPRFEKVDETIYPEYYRKNDSNTRGSNIDTGEPFCVGYIAAVTAASEGPLVVPQDIYLKVNVLLRPENTSSKFPQHEDTNVLYWTTEIREIPFSTVVGHCHLIYEQNVPQNISLQEWLGNDPCRFYFRMAYCKSTGEFTDLPQNAISVGRTDRTKDKGKGKGKSTKTIETVPAKVVEEKIRPLRTLDVFAGCGGLSEGLHQAGVAECKWAIENVEAASHAYSLNNKSCIVFNEDCNALLKTVMSGAKHSANGLRLPMQGEVELLCGGPPCQGFSGMNRFNSREYSNFKNSLVASYLSFCDYYRPKYFILENVRNFVAFKKGMVLKLTLRALLDMGYQCTFGILQAGNYGVPQTRRRLIILAAAPGYNLPFYPEPTHVFSRRACTLTTTIDGKRFSTNIHWDESAPKRTCTIQDAMSDLPQICNGANKIEIEYGSMPESHFQRLVRSNDENSKLRDHICKNMAPLIQARISRIPTTPGSDWRDLPNISVTLSDGTKCKVLQYRYDDKRNGRSSSGAVRGVCACASGRACSPLDKQENTLIPWCLPHTGNRHNHWAGLYGRLSWGGYFSTTVTDPEPMGKQGRVLHPDQHRVVSVRECARSQGFPDTYLFAGSVQDKHRQIGNAVPPPLGAALGREIKKALTLSLTTS

Target ORF information:

RefSeq Version	XM_012695406.2
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori DNA cytosine-5 methyltransferase (Dnmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012695406.2

ORF Insert Sequence:

ATGAGGTGCA TGGATAAGAT ACTTGATGGG GATGAAATAA TCAAAGAAAA CTCTAAGAGA 
AAAAGGAGCT GTGAAGAAAT TGTTTCTGCC AATAATAAAC GATCAAAAAC AGAAGACAAT 
GATGGACATC AAATTTCACA AGATTCTACA AGCAATGACT CTATTGAAAA TCTTAACATT 
CCAGCAAAAA ATACTAGTCT TATTGTTTCT GAAAACATCA ATAATGTATA TAAAAATTAT 
GACGAGGTTG AAAATAAACC AGTATATAAA AATGGTGACC CTATCGTTGT TGAAATAGAT 
AACAAGGATA AGCCTGGATC AGTTAACAAC AATCATGACA TGATTGATGA TTCAGAGACC 
ATATCTAATA TTAAGAATAG CAATAATATT ATCCCTGATA CAGAGAAATG TAACATATGT 
GGCCAATTTT TAAATAATTC TGACTTAATT TATTACCAAG GTCACCCACA AGATGCTGTT 
GAAGAATATA TTGCATTGAC TAATGACAAA CTTGTTTTGA GCTCTGGGGA AGATGGAGAC 
ATAATGGAAC GACCCCAAAC AAATATAACT GGCTTTACAA TATTTGATGA GCAAGGACAT 
TTGTGTCCAA TTGATGGTGG TTTGGTAGAA AACGATGTTC GAATTTATAT GTCTGGATAT 
TTAAAGTCAA TTTGCTCTGA TTCATCAGAG ATTGACGAAG AATCCATTCC AGTTAAAGAT 
GTAGGTCCTA TAATAGAATG GTTCATACAT GGTTTCGATG GTGGAGATAG GAACTGTATA 
ACTTTATCAA CGGAATTTGG GGAATATAAC TTGCTCAAAC CTAGTGAAGC TTATACGCCC 
CTAATGAATA ATTTATATGA AAAAATATGG CTTAGCAAAG TAGTCGTCGA ATTCTTAGAA 
GAATATCACT ACTTGCAACC GAGCTATGAA GATTTACTTG AAGTTGTAAG AGATTTTTCC 
ATTCCTGAAC TAAATAATAA AAAGATGACA GAAGAAATGC TACATAAACA TGCCCAGTTT 
GTTTGTGATC AAGTAGTTAG CTTAGAAATA GAGGAAGATG ATGAACCATT AATAACATTA 
CCTTGTATGA GAGAACTTAT CAAATTAATG GGCATAAAAT TCGGTAAACG AAAAATACGT 
ACCCAGATAC AATACAAAAA AACAGATAAG AAAGCATGGA CAAAAGCTAC AACAACTCCA 
CTTGTAAGAA AAACATTTGA AAGTTTTTTC TCAAATCAGT TAGACAAAAC CAATCACGAA 
CTGGTCTTAC GAAGAAAACG ATGCGGGGTT TGTGAAGCCT GTCAATTGCC TGATTGCGGC 
GAATGTAATG CATGTCGTGC CATGGCTAAA TTCGGTGGTC ATGGAAGAAC TAAGAAAGCA 
TGCGTCCGTA GATTATGTCC TAATATGGCA GTAGAACAGG CAGAAGATTC TGATCCCGAT 
GATGAAGATG AGTATCAGCA AATATCTGAG AAAAAACAAG ACAAAATAGA TGATGCAGTA 
CCAGTCAAGC TCACTGGGTC AAATAGTAAA AATTTAAAAT GGATTGGTGA GCCTGTTAAA 
GCTGATGCCA CAAAAATATA TTACGAAAAA GTTGAAATTG ATGGTGCTGA GCTGTGTAAT 
GGTGACTTTG TCATGATTGA AACTTCCCAA ACAAACATTC CTACACTGGT CGCTAAAGTT 
GTATACATGT GGAAAGAAAT CCATAACCCG AAATCAGGCT ATTTTCACGG AGAAGTCTTC 
ATACGAGCCT CGGACACAGT TTTAGGAGAA GTGAGTGACC CAAGAGAAGT ATTTTTGGGC 
GACAGATGTT GCCATGGTGC TCCGCTTTCT TCCATATTAA GAAAAGCGAA TATAGAAAGG 
AAAGAGACGT CAGCAGATTG GTTTAAACTT GGCGGGAAAG AAGTAGACGA CGAACATTTC 
GAGGATGATG GAAGAACTTA TTTTTATAGC AAGTATTACG ACAGGTTCAC GTCGCGTTTT 
GAAGATTTAC CTCCAGATCC AGCGTGTCCA AATGCTTTAA GAAAGCACAG ATTCTGCCCA 
TCTTGTGAAC GTAAAACAAA ACGAGATGCA AGAAACATTC CTAAAGTTTT TGAAAAATTG 
ATTGTGAAAT CCGAAATTGT TTCAGAACAA AACAGGTCGG AATGGTCATA TGTAAAATGG 
CAAGACTTTG ACTATAAGAA GGGTTGTGGT GTTTTCCTAA AACCGGGAAC CTTTAAACTA 
AAAAATAGTA TGACAAAAGC GAACACTGTT GCCAAACCGC GGTTTGAAAA AGTCGATGAA 
ACTATTTATC CTGAATATTA TAGGAAGAAC GATTCTAATA CGCGTGGGTC TAATATTGAT 
ACCGGGGAAC CTTTTTGTGT GGGTTACATA GCTGCCGTCA CTGCCGCGAG TGAGGGACCT 
CTAGTTGTAC CGCAAGATAT TTACCTGAAA GTGAATGTGC TGCTTAGACC TGAAAACACG 
AGCAGTAAAT TTCCGCAGCA CGAGGACACT AATGTTTTAT ATTGGACAAC TGAAATAAGG 
GAGATTCCAT TTTCGACCGT CGTTGGGCAT TGTCATTTAA TTTATGAACA AAATGTTCCC 
CAAAATATTT CACTTCAAGA ATGGTTAGGA AATGATCCGT GCAGATTCTA CTTCAGAATG 
GCGTATTGTA AGTCCACGGG AGAGTTCACG GATCTCCCGC AGAATGCCAT CAGTGTTGGA 
AGAACAGATC GTACTAAAGA TAAAGGGAAG GGGAAGGGAA AATCTACAAA AACTATTGAA 
ACGGTTCCCG CTAAAGTTGT AGAGGAGAAA ATTAGACCGT TGCGAACTTT AGACGTGTTT 
GCCGGTTGTG GGGGCCTATC AGAAGGCTTG CATCAAGCTG GAGTTGCAGA ATGCAAGTGG 
GCTATTGAAA ACGTGGAAGC TGCTTCTCAT GCTTATTCAT TAAATAATAA AAGTTGTATT 
GTATTTAATG AAGACTGTAA TGCACTTTTG AAAACTGTAA TGTCTGGTGC TAAGCACAGC 
GCGAATGGAC TGCGGCTCCC CATGCAAGGA GAAGTGGAGC TTTTATGTGG CGGGCCACCA 
TGTCAAGGCT TCTCCGGAAT GAATCGTTTT AATTCAAGAG AGTATTCAAA CTTCAAAAAC 
TCATTAGTTG CATCGTATTT ATCGTTTTGT GATTATTACA GACCTAAATA TTTTATACTG 
GAAAACGTTC GTAACTTTGT GGCCTTTAAA AAAGGTATGG TTTTGAAACT CACTCTGCGA 
GCTTTGTTGG ACATGGGATA CCAGTGTACC TTTGGTATCT TACAAGCGGG TAACTACGGA 
GTTCCCCAAA CTAGACGAAG GTTGATCATA TTGGCGGCAG CGCCGGGTTA CAATCTTCCG 
TTCTACCCAG AACCCACGCA CGTCTTCAGT AGACGTGCTT GTACATTAAC AACGACGATT 
GACGGCAAAA GGTTCTCGAC TAATATTCAC TGGGACGAGT CGGCTCCAAA AAGAACGTGC 
ACCATTCAAG ACGCCATGAG TGATTTGCCT CAAATATGTA ATGGTGCTAA TAAAATAGAA 
ATCGAATATG GCTCCATGCC AGAAAGTCAT TTCCAGAGAT TAGTGCGAAG CAACGATGAG 
AATTCTAAAC TACGAGATCA CATCTGTAAA AACATGGCGC CATTGATTCA AGCTAGGATC 
AGCAGGATAC CTACAACACC GGGCTCCGAC TGGAGAGATC TACCCAACAT ATCGGTTACT 
CTCTCCGATG GCACTAAGTG CAAGGTTCTA CAGTATCGCT ACGACGACAA GCGCAATGGG 
CGGTCGAGCA GCGGCGCAGT GCGCGGCGTG TGCGCGTGCG CGAGCGGCCG CGCCTGCTCG 
CCGCTGGACA AGCAGGAGAA CACGCTCATC CCGTGGTGTC TGCCGCACAC CGGCAACAGG 
CACAACCACT GGGCCGGGCT CTACGGTCGG CTATCGTGGG GCGGGTACTT CAGCACGACA 
GTGACGGATC CCGAACCGAT GGGCAAGCAA GGCCGGGTGC TCCACCCCGA CCAGCACCGA 
GTCGTCTCCG TGCGAGAGTG CGCGCGCTCG CAGGGCTTCC CTGATACGTA TCTCTTCGCA 
GGCTCCGTAC AGGACAAACA TCGACAGATC GGAAATGCAG TTCCACCACC TCTTGGCGCC 
GCTCTGGGTA GAGAAATAAA GAAAGCGCTT ACTCTCAGCC TGACCACATC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA methyltransferase BmDnmt1 and BmDnmt2 in silkworm (Bombyx mori) and the regulation of silkworm embryonic development.
Archives of insect biochemistry and physiology100(3)e21529(2019 Mar)
Li B,Hu P,Zhang SZ,Toufeeq S,Wang J,Zhao K,Xu X,Xu JP,Huang SJ

DNA methylation mediates BmDeaf1-regulated tissue- and stage-specific expression of BmCHSA-2b in the silkworm, Bombyx mori.
Epigenetics & chromatin11(1)32(2018 06)
Xu G,Zhang J,Lyu H,Song Q,Feng Q,Xiang H,Zheng S

Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori.
Insect biochemistry and molecular biology5855-65(2015 Mar)
Mitsudome T,Mon H,Xu J,Li Z,Lee JM,Patil AA,Masuda A,Iiyama K,Morokuma D,Kusakabe T

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

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Dnmt1 cDNA ORF clone, Bombyx mori(domestic silkworm)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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