NOS1 cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS1
Entrez Gene ID	692510
Full Name	nitric oxide synthase 1
Synonyms	BmNOS,BmNOS1,NOS,iNOS-LP
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043498.1	NP_001036963.1	nitric oxide synthase
XM_012690766.1	XP_012546220.1	nitric oxide synthase isoform X1
XM_012690766.2	XP_012546220.1	nitric oxide synthase isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Success in the acquisition of Bombyx mori sperm motility is influenced by the extracellular production of nitric oxide (NO) in the presence of seminal fluid nitric oxide synthase (NOS).
Nagaoka S, Asagoshi M, Kato K, Takata Y
Insect biochemistry and molecular biology8440-47(2017 05)

Nitric oxide synthase during early embryonic development in silkworm Bombyx mori: Gene expression, enzyme activity, and tissue distribution.
Kitta R, Kuwamoto M, Yamahama Y, Mase K, Sawada H
Development, growth & differentiation58(9)750-756(2016 Dec)

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori.
Tanaka H, Ishibashi J, Fujita K, Nakajima Y, Sagisaka A, Tomimoto K, Suzuki N, Yoshiyama M, Kaneko Y, Iwasaki T, Sunagawa T, Yamaji K, Asaoka A, Mita K, Yamakawa M
Insect biochemistry and molecular biology38(12)1087-110(2008 Dec)

cDNA cloning, characterization and gene expression of nitric oxide synthase from the silkworm, Bombyx mori.
Imamura M, Yang J, Yamakawa M
Insect molecular biology11(3)257-65(2002 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBg13838	NM_001043498.1 Latest version!	Bombyx mori nitric oxide synthase 1 (NOS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBg13908	XM_012690766.2 Latest version!	Bombyx mori nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OBg13908	XM_012690766.1	Bombyx mori nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS1 ( NM_001043498.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001036963.1 Bombyx mori nitric oxide synthase 1 (NOS1), mRNA.

Price & Availability↑

CloneID	OBg13838
Clone ID Related Accession (Same CDS sequence)	NM_001043498.1
Accession Version	NM_001043498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Bombyx mori(domestic silkworm)
Product	nitric oxide synthase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB071182.1. ##Evidence-Data-START## Transcript exon combination :: AB071182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00024048, SAMD00024050 [ECO:0000348] ##Evidence-Data-END##

ATGTCAAATT TCTTTAAGAT TTGCTGCGTG AAGGATCCCG GTGGATCCGC GAAAGAGAAC 
CTCAGGAAGA TGGATCAAGT TAACGGGCAT TTCGTTTCGG CTACGTGCCC TTTTAGTGGT 
GAAAGTGTTG AGTTGAAAGT TGACAGTAAT CAGAAACAAG TGAAGCCAAA TCTTCGTATA 
AAAGTTCCTC AACCGATAAG ACTAAAGAAC CATGTAGCTC ACGACGAGAA CTTCGACACG 
CTTCACTCCC GGATCAATGA AGTCACTCAT TTTAACACAA AATGCTCAGA GAAAGTTTGT 
CAGACAAGCA TTATGGATAT ACCTGGTCGA GGTGATACCC CGCGGACGGC GGAAGAAGTG 
TTCAAGGATG CTCAGGCATT CCTCACACAA TACTACGCTT CAATCAAGCG AGAGAACTCA 
GAAGCCCACA AAGCGAGGTT AGACGAAGTG AAGAGGGAAT TGAAAGAAAG AGGAACGTAT 
CAACTTAAAA CTTCGGAATT GGTGTTCGGA GCCAAGTTGG CTTGGCGTAA TGCGACCAGG 
TGCATCGGAA GAATACAGTG GAAAAAGTTA CAGATATTCG ATTGCAGAGA AGTGACCACA 
GCAAGCGGTA TGTTCGAAGC ATTGTGCAAT CACATCAAAT ACGCTACGAA TAAAGGAAAT 
ATTCGTTCAG CAATAACGAT CTTCCCACAA CGCACAGACG GTAAACATGA CTACAGAATA 
TGGAATCCGC AGCTTATCAA TTATGCAGGA TACCAGGAGC CAGATGGAAG CATTACAGGA 
GATCCAGCAC GAGTTGAATT TACAGAGATT TGTATGAAAC TTGGATGGAA AGCGCCGCGC 
ACCCCATGGG ACATTTTGCC GCTCGTACTC TCTGCTGACG GAAAAGATCC TGAATTCTTT 
GAACTACCCA AAGAAATAGT TATGGAAGTG CAATTCGAAC ATCCTGAATA TGACTGGTTC 
AAAGACATGG GTTGGAAATG GTATGCTTTG CCGGCTGTTT CCAACATGAG GCTTGCCTGC 
GGGGGCCTAG AGTTCACAGC TAATTCCTTC AACGGCTGGT ACATGGGCAC TGAGATCGGA 
TGCAGGAACC TCTGCGATGA GAGTCGACTG AATATTGTTG AGGCTGTAGC CAAGAAAATG 
GGTCTAGACA CAAACTCGTT TGTTTCTCTG TGGAAAGATA AGGCATTAGT TGAAGTTAAT 
ATAGCGGTAC TGCACAGTTT CCATAGAGAT AACGTGTCCA TTGTTGATCA TCACTCCGCG 
TCCGAACAGT TCCAGAAGCA TTTAGAAAAT GAAAATAAAA GCAGGGGCGG ATGTCCAGCT 
GATTGGATAT GGATCGTTCC ACCGATGTCT TCATCGTTAA CCACAGTATT CCACCAAGAA 
ATGGCCTTGT ATTACATCAG ACCGTCATAT GATTATCAGG AACCACCATG GAAGACTCAC 
CAATGGACAA AGTCTGACGG CACTAAGACC GTCCACAGGA AGTTCCACTT CAAACAGATC 
GCCAGAGCAG TTAAGTTCAC TTCCAAACTG TTCGGACGGG CGCTCTCCAA GCGCATCAAA 
GCGACTATTC TCTACGCAAC AGAGACGGGC AAATCTGAAC ACTACGCCAA AGAATTAGGA 
ACTATTTTCG GACACGCTTT CAACGCGCAG GTACATTGCA TGTCGGAATA TGATATGTTT 
TCTATAGAAC ATGAGACTTT AGTTCTCATA GTGGCTTCCA CGTTCGGCAA TGGAGAACCT 
CCAGCTAACG GAGTCGATTT TGCAGAGCAT CTATTTCAAA TGCTTTATAA TGAGACAAAC 
AATAATCGAG GAGATGGTAC AAGTGACTTA GGCTCTGGTA CGTTCAAGAC ACCAACACCA 
AAATCACTGA TGAGATCAAA CAGTATGATG ACACCGAGCT TTGAATATAA GCGGCAATTA 
ACGAGATTAG AGTCTAACAA AAGTAGCGTG GCCGGTGCAT CATCTATAGA ACAAATAGGA 
CCTCTTAGCA ACGTTTGTTT CGCTGTATTT GGTCTGGGGT CGAGTGCGTA CCCAAAATTC 
TGTCATTTCG GTAAAACAGT TGACAAAATC CTTGGTGATT TAGGCGGCGA AAGAATATTA 
GAACTGGCAT GTGGTGATGA ATTGTACGGA CAAGAACAAC AGTTTCGCGC TTGGTCTTCG 
AAAATATTTC AGGTTGCCTG TGAAACGTTT TGCTTAGATG AAAACGGCAT GGTAAAAGAT 
GCAAAGAAAG CTTTGGGTGA CGTACCGCTG ACAGAAGAGA CAGTACGTTT CGGAAAATAT 
CAGGGAGATA CAAAGCTAAA GGCAGCTCTG GAGGCCAGCT TCAGAAAACA GCTCATTACT 
TGTAAAGTTA AAGAGAATAA AAACCTAGGG GACTTCTCAG CGGATCGGGC CACAGTATTT 
GTTGATATGC AACCAGAAAC TGAATTCAAA TACGATCCAG GAGATCACGT CGGAGTTTTG 
GCTTGTAATA GAAAAGAAAT AGTAGATGCA GTACTAGAGA GGATGAAAGA TGTTGACGAC 
TATGATAAAA CTGTTCAATT ACAAGTGATG AAGGAGACGT TGACACCTAC AGGTGCTATA 
AAGACATGGG AGCAACATGA AAGATTACCA GCGGTAACGA TACGACAGAT ATTTACGAGA 
TTTCTTGACA TCACGACACC ACCGTCAACG ACTGTACTGA AATACTTATC TAAAGCTTGC 
ACTGACCAGA ATGATGCTGC CCAACTTAAA GAATTAGCCA TAGATTCCAA CAAATACGAC 
GACTGGAGGC ATCTCCACTA TCCACACTTG GCCGAAGTAC TAGCGCAGTT CCCTTCATGT 
CGACCTCAAG CATCTTTGCT AGCGGCCCTA CTACCATCGT TACAGCCGAG GTTCTACTCC 
ATATCTTCAT CACCACTAGC GCATCCTCAT AGAATTCACA TCACATGCGC TGTTGTTGTT 
TACAGGACAC AGAACGGACA AGGCCCGATG CATTATGGAG TATGCTCTAC ATACCTACAA 
AATCTAAAAC CGGAGGACGA AGCCCTAGTT TTCATAAGAC GTGCACCAAG CTTCCATATG 
CCTAAAGATT TATCAGCACC GCTTATTCTT GTCGGCCCCG GCTCTGGTAT AGCGCCGTTC 
AGAGGATTTT GGCATCACAG AAAGCATCAG ATCAAGAATT TGATACCGAA CAAACAAAAA 
CCAGGTCCTG TCTGGTTGTT CTTCGGTTGC CGAAACAGAG GAATGGATTT GTATAAAGAA 
GAAAAAGAAA AAGCGTTAGC CGATGGAGTA TTGAGCAAAG TTTTCTTGGC ACTTTCTAGG 
GAAGACTCTG TTGAAAAGAA ACACATCCAA ACGCTACTGG AGGACGAGGG TGCCGAAATC 
TCACGGATGC TTCTAGACGA AAACGGACAT TTCTACGTTT GTGGTGATTG TAAAATGGCT 
GAAGAAGTTC AACAGAAATT AAAAGAAATC ATGAAGAAAC ATGCCAAAAT GACTGAGCAA 
GAATATGAAG AGTTCATCTT GAACTTGATG GATGAAAACA GATACCACGA AGATATCTTC 
GGGATCACTC TGAGAACAGC AGAAGTTCAA AGTGCATCAA GAGAATTCGC TAAAAGGACA 
AGGCAGGAAT CACAGAAGTC ACAAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036963.1
CDS	224..3853
Translation	MSNFFKICCVKDPGGSAKENLRKMDQVNGHFVSATCPFSGESVELKVDSNQKQVKPNLRIKVPQPIRLKNHVAHDENFDTLHSRINEVTHFNTKCSEKVCQTSIMDIPGRGDTPRTAEEVFKDAQAFLTQYYASIKRENSEAHKARLDEVKRELKERGTYQLKTSELVFGAKLAWRNATRCIGRIQWKKLQIFDCREVTTASGMFEALCNHIKYATNKGNIRSAITIFPQRTDGKHDYRIWNPQLINYAGYQEPDGSITGDPARVEFTEICMKLGWKAPRTPWDILPLVLSADGKDPEFFELPKEIVMEVQFEHPEYDWFKDMGWKWYALPAVSNMRLACGGLEFTANSFNGWYMGTEIGCRNLCDESRLNIVEAVAKKMGLDTNSFVSLWKDKALVEVNIAVLHSFHRDNVSIVDHHSASEQFQKHLENENKSRGGCPADWIWIVPPMSSSLTTVFHQEMALYYIRPSYDYQEPPWKTHQWTKSDGTKTVHRKFHFKQIARAVKFTSKLFGRALSKRIKATILYATETGKSEHYAKELGTIFGHAFNAQVHCMSEYDMFSIEHETLVLIVASTFGNGEPPANGVDFAEHLFQMLYNETNNNRGDGTSDLGSGTFKTPTPKSLMRSNSMMTPSFEYKRQLTRLESNKSSVAGASSIEQIGPLSNVCFAVFGLGSSAYPKFCHFGKTVDKILGDLGGERILELACGDELYGQEQQFRAWSSKIFQVACETFCLDENGMVKDAKKALGDVPLTEETVRFGKYQGDTKLKAALEASFRKQLITCKVKENKNLGDFSADRATVFVDMQPETEFKYDPGDHVGVLACNRKEIVDAVLERMKDVDDYDKTVQLQVMKETLTPTGAIKTWEQHERLPAVTIRQIFTRFLDITTPPSTTVLKYLSKACTDQNDAAQLKELAIDSNKYDDWRHLHYPHLAEVLAQFPSCRPQASLLAALLPSLQPRFYSISSSPLAHPHRIHITCAVVVYRTQNGQGPMHYGVCSTYLQNLKPEDEALVFIRRAPSFHMPKDLSAPLILVGPGSGIAPFRGFWHHRKHQIKNLIPNKQKPGPVWLFFGCRNRGMDLYKEEKEKALADGVLSKVFLALSREDSVEKKHIQTLLEDEGAEISRMLLDENGHFYVCGDCKMAEEVQQKLKEIMKKHAKMTEQEYEEFILNLMDENRYHEDIFGITLRTAEVQSASREFAKRTRQESQKSQT

Target ORF information:

RefSeq Version	NM_001043498.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori nitric oxide synthase 1 (NOS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043498.1

ORF Insert Sequence:

ATGTCAAATT TCTTTAAGAT TTGCTGCGTG AAGGATCCCG GTGGATCCGC GAAAGAGAAC 
CTCAGGAAGA TGGATCAAGT TAACGGGCAT TTCGTTTCGG CTACGTGCCC TTTTAGTGGT 
GAAAGTGTTG AGTTGAAAGT TGACAGTAAT CAGAAACAAG TGAAGCCAAA TCTTCGTATA 
AAAGTTCCTC AACCGATAAG ACTAAAGAAC CATGTAGCTC ACGACGAGAA CTTCGACACG 
CTTCACTCCC GGATCAATGA AGTCACTCAT TTTAACACAA AATGCTCAGA GAAAGTTTGT 
CAGACAAGCA TTATGGATAT ACCTGGTCGA GGTGATACCC CGCGGACGGC GGAAGAAGTG 
TTCAAGGATG CTCAGGCATT CCTCACACAA TACTACGCTT CAATCAAGCG AGAGAACTCA 
GAAGCCCACA AAGCGAGGTT AGACGAAGTG AAGAGGGAAT TGAAAGAAAG AGGAACGTAT 
CAACTTAAAA CTTCGGAATT GGTGTTCGGA GCCAAGTTGG CTTGGCGTAA TGCGACCAGG 
TGCATCGGAA GAATACAGTG GAAAAAGTTA CAGATATTCG ATTGCAGAGA AGTGACCACA 
GCAAGCGGTA TGTTCGAAGC ATTGTGCAAT CACATCAAAT ACGCTACGAA TAAAGGAAAT 
ATTCGTTCAG CAATAACGAT CTTCCCACAA CGCACAGACG GTAAACATGA CTACAGAATA 
TGGAATCCGC AGCTTATCAA TTATGCAGGA TACCAGGAGC CAGATGGAAG CATTACAGGA 
GATCCAGCAC GAGTTGAATT TACAGAGATT TGTATGAAAC TTGGATGGAA AGCGCCGCGC 
ACCCCATGGG ACATTTTGCC GCTCGTACTC TCTGCTGACG GAAAAGATCC TGAATTCTTT 
GAACTACCCA AAGAAATAGT TATGGAAGTG CAATTCGAAC ATCCTGAATA TGACTGGTTC 
AAAGACATGG GTTGGAAATG GTATGCTTTG CCGGCTGTTT CCAACATGAG GCTTGCCTGC 
GGGGGCCTAG AGTTCACAGC TAATTCCTTC AACGGCTGGT ACATGGGCAC TGAGATCGGA 
TGCAGGAACC TCTGCGATGA GAGTCGACTG AATATTGTTG AGGCTGTAGC CAAGAAAATG 
GGTCTAGACA CAAACTCGTT TGTTTCTCTG TGGAAAGATA AGGCATTAGT TGAAGTTAAT 
ATAGCGGTAC TGCACAGTTT CCATAGAGAT AACGTGTCCA TTGTTGATCA TCACTCCGCG 
TCCGAACAGT TCCAGAAGCA TTTAGAAAAT GAAAATAAAA GCAGGGGCGG ATGTCCAGCT 
GATTGGATAT GGATCGTTCC ACCGATGTCT TCATCGTTAA CCACAGTATT CCACCAAGAA 
ATGGCCTTGT ATTACATCAG ACCGTCATAT GATTATCAGG AACCACCATG GAAGACTCAC 
CAATGGACAA AGTCTGACGG CACTAAGACC GTCCACAGGA AGTTCCACTT CAAACAGATC 
GCCAGAGCAG TTAAGTTCAC TTCCAAACTG TTCGGACGGG CGCTCTCCAA GCGCATCAAA 
GCGACTATTC TCTACGCAAC AGAGACGGGC AAATCTGAAC ACTACGCCAA AGAATTAGGA 
ACTATTTTCG GACACGCTTT CAACGCGCAG GTACATTGCA TGTCGGAATA TGATATGTTT 
TCTATAGAAC ATGAGACTTT AGTTCTCATA GTGGCTTCCA CGTTCGGCAA TGGAGAACCT 
CCAGCTAACG GAGTCGATTT TGCAGAGCAT CTATTTCAAA TGCTTTATAA TGAGACAAAC 
AATAATCGAG GAGATGGTAC AAGTGACTTA GGCTCTGGTA CGTTCAAGAC ACCAACACCA 
AAATCACTGA TGAGATCAAA CAGTATGATG ACACCGAGCT TTGAATATAA GCGGCAATTA 
ACGAGATTAG AGTCTAACAA AAGTAGCGTG GCCGGTGCAT CATCTATAGA ACAAATAGGA 
CCTCTTAGCA ACGTTTGTTT CGCTGTATTT GGTCTGGGGT CGAGTGCGTA CCCAAAATTC 
TGTCATTTCG GTAAAACAGT TGACAAAATC CTTGGTGATT TAGGCGGCGA AAGAATATTA 
GAACTGGCAT GTGGTGATGA ATTGTACGGA CAAGAACAAC AGTTTCGCGC TTGGTCTTCG 
AAAATATTTC AGGTTGCCTG TGAAACGTTT TGCTTAGATG AAAACGGCAT GGTAAAAGAT 
GCAAAGAAAG CTTTGGGTGA CGTACCGCTG ACAGAAGAGA CAGTACGTTT CGGAAAATAT 
CAGGGAGATA CAAAGCTAAA GGCAGCTCTG GAGGCCAGCT TCAGAAAACA GCTCATTACT 
TGTAAAGTTA AAGAGAATAA AAACCTAGGG GACTTCTCAG CGGATCGGGC CACAGTATTT 
GTTGATATGC AACCAGAAAC TGAATTCAAA TACGATCCAG GAGATCACGT CGGAGTTTTG 
GCTTGTAATA GAAAAGAAAT AGTAGATGCA GTACTAGAGA GGATGAAAGA TGTTGACGAC 
TATGATAAAA CTGTTCAATT ACAAGTGATG AAGGAGACGT TGACACCTAC AGGTGCTATA 
AAGACATGGG AGCAACATGA AAGATTACCA GCGGTAACGA TACGACAGAT ATTTACGAGA 
TTTCTTGACA TCACGACACC ACCGTCAACG ACTGTACTGA AATACTTATC TAAAGCTTGC 
ACTGACCAGA ATGATGCTGC CCAACTTAAA GAATTAGCCA TAGATTCCAA CAAATACGAC 
GACTGGAGGC ATCTCCACTA TCCACACTTG GCCGAAGTAC TAGCGCAGTT CCCTTCATGT 
CGACCTCAAG CATCTTTGCT AGCGGCCCTA CTACCATCGT TACAGCCGAG GTTCTACTCC 
ATATCTTCAT CACCACTAGC GCATCCTCAT AGAATTCACA TCACATGCGC TGTTGTTGTT 
TACAGGACAC AGAACGGACA AGGCCCGATG CATTATGGAG TATGCTCTAC ATACCTACAA 
AATCTAAAAC CGGAGGACGA AGCCCTAGTT TTCATAAGAC GTGCACCAAG CTTCCATATG 
CCTAAAGATT TATCAGCACC GCTTATTCTT GTCGGCCCCG GCTCTGGTAT AGCGCCGTTC 
AGAGGATTTT GGCATCACAG AAAGCATCAG ATCAAGAATT TGATACCGAA CAAACAAAAA 
CCAGGTCCTG TCTGGTTGTT CTTCGGTTGC CGAAACAGAG GAATGGATTT GTATAAAGAA 
GAAAAAGAAA AAGCGTTAGC CGATGGAGTA TTGAGCAAAG TTTTCTTGGC ACTTTCTAGG 
GAAGACTCTG TTGAAAAGAA ACACATCCAA ACGCTACTGG AGGACGAGGG TGCCGAAATC 
TCACGGATGC TTCTAGACGA AAACGGACAT TTCTACGTTT GTGGTGATTG TAAAATGGCT 
GAAGAAGTTC AACAGAAATT AAAAGAAATC ATGAAGAAAC ATGCCAAAAT GACTGAGCAA 
GAATATGAAG AGTTCATCTT GAACTTGATG GATGAAAACA GATACCACGA AGATATCTTC 
GGGATCACTC TGAGAACAGC AGAAGTTCAA AGTGCATCAA GAGAATTCGC TAAAAGGACA 
AGGCAGGAAT CACAGAAGTC ACAAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_012690766.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_012546220.1 Bombyx mori nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBg13908
Clone ID Related Accession (Same CDS sequence)	XM_012690766.1 , XM_012690766.2
Accession Version	XM_012690766.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433347200000
Organism	Bombyx mori(Domestic silkworm)
Product	nitric oxide synthase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004581679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAAG TTAACGGGCA TTTCGTTTCG GCTACGTGCC CTTTTAGTGG TGAAAGTGTT 
GAGTTGAAAG TTGACAGTAA TCAGAAACAA GTGAAGCCAA ATCTTCGTAT AAAAGTTCCT 
CAACCGATAA GACTAAAGAA CCATGTAGCT CACGACGAGA ACTTCGACAC GCTTCACTCC 
CGGATCAATG AAGTCACTCA TTTTAACACC AAATGCTCAG AAAAAGTTTG TCAGACAAGC 
ATTATGGATA TACCTGGTCG AGGTGATACC CCGCGGACGG CGGAAGAAGT GTTCAAGGAT 
GCTCAGGCAT TCCTCACACA ATACTACGCT TCAATCAAGC GAGAGAACTC AGAAGCCCAC 
AAAGCGAGGT TAGACGAAGT GAAGAGGGAA TTGAAAGAAA AAGGAACGTA TCAACTTAAA 
ACTTCGGAAT TGGTGTTCGG GGCCAAGTTG GCTTGGCGTA ATGCGACCAG GTGCATCGGA 
AGAATACAGT GGAAAAAGTT ACAGATATTC GATTGCAGAG AAGTGACCAC AGCAAGCGGT 
ATGTTCGAAG CATTGTGCAA TCACATCAAA TACGCTACGA ATAAAGGAAA TATTCGTTCA 
GCAATAACGA TCTTCCCACA ACGCACCGAC GGTAAACATG ACTACAGAAT ATGGAATCCG 
CAGCTTATCA ATTATGCAGG ATACCAGGAG CCAGATGGAA GCATTATAGG AGATCCAGCA 
CGAGTTGAAT TTACAGAGAT TTGTATGAAA CTTGGATGGA AAGCGCCGCG CACCCCATGG 
GACATTTTGC CGCTCGTACT CTCTGCTGAC GGAAAAGATC CTGAATTCTT TGAACTACCC 
AAAGAAATAG TTATGGAAGT GCAATTCGAA CATCCTGAAT ATGACTGGTT CAAAGACATG 
GGTTGGAAAT GGTATGCTTT GCCGGCTGTT TCCAACATGA GGCTTGACTG CGGGGGCCTA 
GAGTTCACAG CTAATTCCTT CAACGGCTGG TACATGGGCA CTGAGATCGG ATGCAGGAAC 
CTCTGCGATG AGAGTCGACT GAATATTGTT GAGGCTGTAG CCAAGAAAAT GGGTCTAGAC 
ACAAACTCGT TTGTTTCTCT GTGGAAAGAT AAGGCATTAG TTGAAGTTAA TATAGCGGTA 
CTGCACAGTT TCCATAGAGA TAACGTGTCC ATTGTTGATC ATCACTCCGC GTCCGAACAG 
TTCCAGAAGC ATTTAGAAAA TGAAAATAAA AGCAGGGGCG GATGTCCAGC TGATTGGATA 
TGGATCGTTC CACCGATGTC TTCATCGTTA ACCACAGTGT TCCACCAAGA AATGGCCTTG 
TATTACATCA GACCGTCATA TGATTATCAG GAACCACCAT GGAAGACTCA CCAATGGACA 
AAGTCTGACG GCACTAAGAC CGTCCACAGG AAGTTCCACT TCAAACAGAT CGCCAGAGCA 
GTTAAGTTCA CTTCCAAACT GTTCGGACGG GCGCTCTCCA AGCGCATCAA AGCGACTATT 
CTCTACGCAA CAGAGACGGG CAAATCTGAA CACTACGCCA AAGAATTAGG AACTATTTTC 
GGACACGCTT TCAACGCGCA GGTACATTGC ATGTCGGAAT ATGATATGTT TTCTATAGAA 
CATGAGACTT TAGTTCTCAT AGTGGCTTCC ACGTTCGGCA ATGGAGAACC TCCAGCTAAC 
GGAGTCGATT TTGCAGAGCA TCTATTTCAA ATGCTTTATA ATGAGACAAA CAATAATCGA 
GGAGATGGTA CAAATGACTT AGGCTCTGGT ACGTTCAAGA CACCAACACC AAAATCACTG 
ATGAGATCAA ACAGTATGAT GACACCGAGC TTTGAATATA AGCGGCAATT AACGAGATTA 
GAGTCTAACA AAAGTAGCGT GGCCGGTGCA TCATCTATAG AACAAATAGG ACCTCTTAGC 
AACGTTTGTT TCGCTGTATT TGGTCTGGGG TCGAGTGCGT ACCCAAAATT CTGTCATTTC 
GGTAAAACAG TTGACAAAAT CCTTGGTGAT TTAGGCGGCG AAAGAATATT AGAACTGGCA 
TGTGGTGATG AATTGTACGG ACAAGAACAA CAGTTTCGCG CTTGGTCTTC GAAAATATTT 
CAGGTTGCCT GTGAAACGTT TTGCTTAGAT GAAAACGACA TGGTAAAAGA TGCAAAGAAA 
GCTTTGGGTG ACGTACCGCT GACAGAAGAG ACAGTACGTT TCGGAAAATA TCAGGGAGAT 
ACAAAGCTAA AGGCAGCTCT GGAGGCCAGC TTCAGAAAAC AGCTCATTAC TTGTAAAGTT 
AAAGAGAATA AAAACCTAGG GGACTTCTCA GCGGATCGGG CCACAGTATT TGTTGATATG 
CAACCAGAAA CTGAATTCAA ATACGATCCA GGAGATCACG TCGGAGTTTT GGCTTGTAAT 
AGAAAAGAAA TAGTAGATGC AGTACTAGAG AGGATGAAAG ATGTTGACGA CTATGATAAA 
ACTGTTCAAT TACAAGTGAT GAAGGAGACG TTGACACCTA CAGGTGCTAT AAAGACATGG 
GAGCAACATG AAAGATTACC AGCGGTAACG ATACGACAGA TATTTACGAG ATTTCTTGAC 
ATCACGACAC CACCGTCAAC GACTGTACTG AAATACTTAT CTAAAGCTTG CACTGACCAG 
AATGATGCTG CCCAACTTAA AGAATTAGCC ATAGATTCCA ACAAATACGA CGACTGGAGG 
CATCTCCACT ATCCACACTT GGCCGAAGTA CTAGCGCAGT TCCCTTCATG TCGACCTCAA 
GCATCTTTGC TAGCGGCCCT ACTACCATCG TTACAGCCGA GGTTCTACTC CATATCTTCA 
TCACCACTAG CGCATCCTCA TAGAATTCAC ATCACATGCG CTGTTGTTGT TTACAGGACA 
CAGAACGGAC AAGGCCCGAT GCATTATGGA GTATGCTCTA CATACCTACA AAATCTAAAA 
CCGGAGGACG AAGCCCTAGT TTTCATAAGA CGTGCACCAA GCTTCCATAT GCCTAAAGAT 
TTATCAGCAC CGCTTATTCT TGTCGGCCCC GGCTCTGGTA TAGCGCCGTT CAGAGGATTT 
TGGCATCACA GAAAGCATCA GATCAAGAAT TTGATACCGA ACAAACAAAA ACCAGGTCCT 
GTCTGGTTGT TCTTCGGTTG CCGAAACAGA GGAATGGATT TGTATAAAGA AGAAAAAGAA 
AAAGCGTTAG CCGATGGAGT ATTGAGCAAA GTTTTCTTGG CACTTTCTAG GGAAGACTCT 
GTTGAAAAGA AACACATCCA AACGCTACTG GAGGACGAGG GTGCCGAAAT CTCACGGATG 
CTTCTAGACG AAAACGGACA TTTCTACGTT TGTGGTGATT GTAAAATGGC TGAAGAAGTT 
CAACAGAAAT TAAAAGAAAT CATGAAGAAA CATGCCAAAA TGACTGAGCA AGAATATGAA 
GAGTTCATCT TGAACTTGAT GGATGAAAAC AGATACCACG AAGATATCTT CGGGATCACT 
CTGAGAACAG CAGAAGTTCA AAGTGCATCA AGAGAATTCG CTAAAAGGAC AAGGCAGGAA 
TCACAGAAGT CACAAACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012546220.1
CDS	190..3750
Translation	MDQVNGHFVSATCPFSGESVELKVDSNQKQVKPNLRIKVPQPIRLKNHVAHDENFDTLHSRINEVTHFNTKCSEKVCQTSIMDIPGRGDTPRTAEEVFKDAQAFLTQYYASIKRENSEAHKARLDEVKRELKEKGTYQLKTSELVFGAKLAWRNATRCIGRIQWKKLQIFDCREVTTASGMFEALCNHIKYATNKGNIRSAITIFPQRTDGKHDYRIWNPQLINYAGYQEPDGSIIGDPARVEFTEICMKLGWKAPRTPWDILPLVLSADGKDPEFFELPKEIVMEVQFEHPEYDWFKDMGWKWYALPAVSNMRLDCGGLEFTANSFNGWYMGTEIGCRNLCDESRLNIVEAVAKKMGLDTNSFVSLWKDKALVEVNIAVLHSFHRDNVSIVDHHSASEQFQKHLENENKSRGGCPADWIWIVPPMSSSLTTVFHQEMALYYIRPSYDYQEPPWKTHQWTKSDGTKTVHRKFHFKQIARAVKFTSKLFGRALSKRIKATILYATETGKSEHYAKELGTIFGHAFNAQVHCMSEYDMFSIEHETLVLIVASTFGNGEPPANGVDFAEHLFQMLYNETNNNRGDGTNDLGSGTFKTPTPKSLMRSNSMMTPSFEYKRQLTRLESNKSSVAGASSIEQIGPLSNVCFAVFGLGSSAYPKFCHFGKTVDKILGDLGGERILELACGDELYGQEQQFRAWSSKIFQVACETFCLDENDMVKDAKKALGDVPLTEETVRFGKYQGDTKLKAALEASFRKQLITCKVKENKNLGDFSADRATVFVDMQPETEFKYDPGDHVGVLACNRKEIVDAVLERMKDVDDYDKTVQLQVMKETLTPTGAIKTWEQHERLPAVTIRQIFTRFLDITTPPSTTVLKYLSKACTDQNDAAQLKELAIDSNKYDDWRHLHYPHLAEVLAQFPSCRPQASLLAALLPSLQPRFYSISSSPLAHPHRIHITCAVVVYRTQNGQGPMHYGVCSTYLQNLKPEDEALVFIRRAPSFHMPKDLSAPLILVGPGSGIAPFRGFWHHRKHQIKNLIPNKQKPGPVWLFFGCRNRGMDLYKEEKEKALADGVLSKVFLALSREDSVEKKHIQTLLEDEGAEISRMLLDENGHFYVCGDCKMAEEVQQKLKEIMKKHAKMTEQEYEEFILNLMDENRYHEDIFGITLRTAEVQSASREFAKRTRQESQKSQT

Target ORF information:

RefSeq Version	XM_012690766.1
Organism	Bombyx mori(Domestic silkworm)
Definition	Bombyx mori nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012690766.1

ORF Insert Sequence:

ATGGATCAAG TTAACGGGCA TTTCGTTTCG GCTACGTGCC CTTTTAGTGG TGAAAGTGTT 
GAGTTGAAAG TTGACAGTAA TCAGAAACAA GTGAAGCCAA ATCTTCGTAT AAAAGTTCCT 
CAACCGATAA GACTAAAGAA CCATGTAGCT CACGACGAGA ACTTCGACAC GCTTCACTCC 
CGGATCAATG AAGTCACTCA TTTTAACACC AAATGCTCAG AAAAAGTTTG TCAGACAAGC 
ATTATGGATA TACCTGGTCG AGGTGATACC CCGCGGACGG CGGAAGAAGT GTTCAAGGAT 
GCTCAGGCAT TCCTCACACA ATACTACGCT TCAATCAAGC GAGAGAACTC AGAAGCCCAC 
AAAGCGAGGT TAGACGAAGT GAAGAGGGAA TTGAAAGAAA AAGGAACGTA TCAACTTAAA 
ACTTCGGAAT TGGTGTTCGG GGCCAAGTTG GCTTGGCGTA ATGCGACCAG GTGCATCGGA 
AGAATACAGT GGAAAAAGTT ACAGATATTC GATTGCAGAG AAGTGACCAC AGCAAGCGGT 
ATGTTCGAAG CATTGTGCAA TCACATCAAA TACGCTACGA ATAAAGGAAA TATTCGTTCA 
GCAATAACGA TCTTCCCACA ACGCACCGAC GGTAAACATG ACTACAGAAT ATGGAATCCG 
CAGCTTATCA ATTATGCAGG ATACCAGGAG CCAGATGGAA GCATTATAGG AGATCCAGCA 
CGAGTTGAAT TTACAGAGAT TTGTATGAAA CTTGGATGGA AAGCGCCGCG CACCCCATGG 
GACATTTTGC CGCTCGTACT CTCTGCTGAC GGAAAAGATC CTGAATTCTT TGAACTACCC 
AAAGAAATAG TTATGGAAGT GCAATTCGAA CATCCTGAAT ATGACTGGTT CAAAGACATG 
GGTTGGAAAT GGTATGCTTT GCCGGCTGTT TCCAACATGA GGCTTGACTG CGGGGGCCTA 
GAGTTCACAG CTAATTCCTT CAACGGCTGG TACATGGGCA CTGAGATCGG ATGCAGGAAC 
CTCTGCGATG AGAGTCGACT GAATATTGTT GAGGCTGTAG CCAAGAAAAT GGGTCTAGAC 
ACAAACTCGT TTGTTTCTCT GTGGAAAGAT AAGGCATTAG TTGAAGTTAA TATAGCGGTA 
CTGCACAGTT TCCATAGAGA TAACGTGTCC ATTGTTGATC ATCACTCCGC GTCCGAACAG 
TTCCAGAAGC ATTTAGAAAA TGAAAATAAA AGCAGGGGCG GATGTCCAGC TGATTGGATA 
TGGATCGTTC CACCGATGTC TTCATCGTTA ACCACAGTGT TCCACCAAGA AATGGCCTTG 
TATTACATCA GACCGTCATA TGATTATCAG GAACCACCAT GGAAGACTCA CCAATGGACA 
AAGTCTGACG GCACTAAGAC CGTCCACAGG AAGTTCCACT TCAAACAGAT CGCCAGAGCA 
GTTAAGTTCA CTTCCAAACT GTTCGGACGG GCGCTCTCCA AGCGCATCAA AGCGACTATT 
CTCTACGCAA CAGAGACGGG CAAATCTGAA CACTACGCCA AAGAATTAGG AACTATTTTC 
GGACACGCTT TCAACGCGCA GGTACATTGC ATGTCGGAAT ATGATATGTT TTCTATAGAA 
CATGAGACTT TAGTTCTCAT AGTGGCTTCC ACGTTCGGCA ATGGAGAACC TCCAGCTAAC 
GGAGTCGATT TTGCAGAGCA TCTATTTCAA ATGCTTTATA ATGAGACAAA CAATAATCGA 
GGAGATGGTA CAAATGACTT AGGCTCTGGT ACGTTCAAGA CACCAACACC AAAATCACTG 
ATGAGATCAA ACAGTATGAT GACACCGAGC TTTGAATATA AGCGGCAATT AACGAGATTA 
GAGTCTAACA AAAGTAGCGT GGCCGGTGCA TCATCTATAG AACAAATAGG ACCTCTTAGC 
AACGTTTGTT TCGCTGTATT TGGTCTGGGG TCGAGTGCGT ACCCAAAATT CTGTCATTTC 
GGTAAAACAG TTGACAAAAT CCTTGGTGAT TTAGGCGGCG AAAGAATATT AGAACTGGCA 
TGTGGTGATG AATTGTACGG ACAAGAACAA CAGTTTCGCG CTTGGTCTTC GAAAATATTT 
CAGGTTGCCT GTGAAACGTT TTGCTTAGAT GAAAACGACA TGGTAAAAGA TGCAAAGAAA 
GCTTTGGGTG ACGTACCGCT GACAGAAGAG ACAGTACGTT TCGGAAAATA TCAGGGAGAT 
ACAAAGCTAA AGGCAGCTCT GGAGGCCAGC TTCAGAAAAC AGCTCATTAC TTGTAAAGTT 
AAAGAGAATA AAAACCTAGG GGACTTCTCA GCGGATCGGG CCACAGTATT TGTTGATATG 
CAACCAGAAA CTGAATTCAA ATACGATCCA GGAGATCACG TCGGAGTTTT GGCTTGTAAT 
AGAAAAGAAA TAGTAGATGC AGTACTAGAG AGGATGAAAG ATGTTGACGA CTATGATAAA 
ACTGTTCAAT TACAAGTGAT GAAGGAGACG TTGACACCTA CAGGTGCTAT AAAGACATGG 
GAGCAACATG AAAGATTACC AGCGGTAACG ATACGACAGA TATTTACGAG ATTTCTTGAC 
ATCACGACAC CACCGTCAAC GACTGTACTG AAATACTTAT CTAAAGCTTG CACTGACCAG 
AATGATGCTG CCCAACTTAA AGAATTAGCC ATAGATTCCA ACAAATACGA CGACTGGAGG 
CATCTCCACT ATCCACACTT GGCCGAAGTA CTAGCGCAGT TCCCTTCATG TCGACCTCAA 
GCATCTTTGC TAGCGGCCCT ACTACCATCG TTACAGCCGA GGTTCTACTC CATATCTTCA 
TCACCACTAG CGCATCCTCA TAGAATTCAC ATCACATGCG CTGTTGTTGT TTACAGGACA 
CAGAACGGAC AAGGCCCGAT GCATTATGGA GTATGCTCTA CATACCTACA AAATCTAAAA 
CCGGAGGACG AAGCCCTAGT TTTCATAAGA CGTGCACCAA GCTTCCATAT GCCTAAAGAT 
TTATCAGCAC CGCTTATTCT TGTCGGCCCC GGCTCTGGTA TAGCGCCGTT CAGAGGATTT 
TGGCATCACA GAAAGCATCA GATCAAGAAT TTGATACCGA ACAAACAAAA ACCAGGTCCT 
GTCTGGTTGT TCTTCGGTTG CCGAAACAGA GGAATGGATT TGTATAAAGA AGAAAAAGAA 
AAAGCGTTAG CCGATGGAGT ATTGAGCAAA GTTTTCTTGG CACTTTCTAG GGAAGACTCT 
GTTGAAAAGA AACACATCCA AACGCTACTG GAGGACGAGG GTGCCGAAAT CTCACGGATG 
CTTCTAGACG AAAACGGACA TTTCTACGTT TGTGGTGATT GTAAAATGGC TGAAGAAGTT 
CAACAGAAAT TAAAAGAAAT CATGAAGAAA CATGCCAAAA TGACTGAGCA AGAATATGAA 
GAGTTCATCT TGAACTTGAT GGATGAAAAC AGATACCACG AAGATATCTT CGGGATCACT 
CTGAGAACAG CAGAAGTTCA AAGTGCATCA AGAGAATTCG CTAAAAGGAC AAGGCAGGAA 
TCACAGAAGT CACAAACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_012690766.2 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_012546220.1 Bombyx mori nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

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CloneID	OBg13908
Clone ID Related Accession (Same CDS sequence)	XM_012690766.1 , XM_012690766.2
Accession Version	XM_012690766.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	nitric oxide synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004581679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 5, 2017 this sequence version replaced XM_012690766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAAG TTAACGGGCA TTTCGTTTCG GCTACGTGCC CTTTTAGTGG TGAAAGTGTT 
GAGTTGAAAG TTGACAGTAA TCAGAAACAA GTGAAGCCAA ATCTTCGTAT AAAAGTTCCT 
CAACCGATAA GACTAAAGAA CCATGTAGCT CACGACGAGA ACTTCGACAC GCTTCACTCC 
CGGATCAATG AAGTCACTCA TTTTAACACC AAATGCTCAG AAAAAGTTTG TCAGACAAGC 
ATTATGGATA TACCTGGTCG AGGTGATACC CCGCGGACGG CGGAAGAAGT GTTCAAGGAT 
GCTCAGGCAT TCCTCACACA ATACTACGCT TCAATCAAGC GAGAGAACTC AGAAGCCCAC 
AAAGCGAGGT TAGACGAAGT GAAGAGGGAA TTGAAAGAAA AAGGAACGTA TCAACTTAAA 
ACTTCGGAAT TGGTGTTCGG GGCCAAGTTG GCTTGGCGTA ATGCGACCAG GTGCATCGGA 
AGAATACAGT GGAAAAAGTT ACAGATATTC GATTGCAGAG AAGTGACCAC AGCAAGCGGT 
ATGTTCGAAG CATTGTGCAA TCACATCAAA TACGCTACGA ATAAAGGAAA TATTCGTTCA 
GCAATAACGA TCTTCCCACA ACGCACCGAC GGTAAACATG ACTACAGAAT ATGGAATCCG 
CAGCTTATCA ATTATGCAGG ATACCAGGAG CCAGATGGAA GCATTATAGG AGATCCAGCA 
CGAGTTGAAT TTACAGAGAT TTGTATGAAA CTTGGATGGA AAGCGCCGCG CACCCCATGG 
GACATTTTGC CGCTCGTACT CTCTGCTGAC GGAAAAGATC CTGAATTCTT TGAACTACCC 
AAAGAAATAG TTATGGAAGT GCAATTCGAA CATCCTGAAT ATGACTGGTT CAAAGACATG 
GGTTGGAAAT GGTATGCTTT GCCGGCTGTT TCCAACATGA GGCTTGACTG CGGGGGCCTA 
GAGTTCACAG CTAATTCCTT CAACGGCTGG TACATGGGCA CTGAGATCGG ATGCAGGAAC 
CTCTGCGATG AGAGTCGACT GAATATTGTT GAGGCTGTAG CCAAGAAAAT GGGTCTAGAC 
ACAAACTCGT TTGTTTCTCT GTGGAAAGAT AAGGCATTAG TTGAAGTTAA TATAGCGGTA 
CTGCACAGTT TCCATAGAGA TAACGTGTCC ATTGTTGATC ATCACTCCGC GTCCGAACAG 
TTCCAGAAGC ATTTAGAAAA TGAAAATAAA AGCAGGGGCG GATGTCCAGC TGATTGGATA 
TGGATCGTTC CACCGATGTC TTCATCGTTA ACCACAGTGT TCCACCAAGA AATGGCCTTG 
TATTACATCA GACCGTCATA TGATTATCAG GAACCACCAT GGAAGACTCA CCAATGGACA 
AAGTCTGACG GCACTAAGAC CGTCCACAGG AAGTTCCACT TCAAACAGAT CGCCAGAGCA 
GTTAAGTTCA CTTCCAAACT GTTCGGACGG GCGCTCTCCA AGCGCATCAA AGCGACTATT 
CTCTACGCAA CAGAGACGGG CAAATCTGAA CACTACGCCA AAGAATTAGG AACTATTTTC 
GGACACGCTT TCAACGCGCA GGTACATTGC ATGTCGGAAT ATGATATGTT TTCTATAGAA 
CATGAGACTT TAGTTCTCAT AGTGGCTTCC ACGTTCGGCA ATGGAGAACC TCCAGCTAAC 
GGAGTCGATT TTGCAGAGCA TCTATTTCAA ATGCTTTATA ATGAGACAAA CAATAATCGA 
GGAGATGGTA CAAATGACTT AGGCTCTGGT ACGTTCAAGA CACCAACACC AAAATCACTG 
ATGAGATCAA ACAGTATGAT GACACCGAGC TTTGAATATA AGCGGCAATT AACGAGATTA 
GAGTCTAACA AAAGTAGCGT GGCCGGTGCA TCATCTATAG AACAAATAGG ACCTCTTAGC 
AACGTTTGTT TCGCTGTATT TGGTCTGGGG TCGAGTGCGT ACCCAAAATT CTGTCATTTC 
GGTAAAACAG TTGACAAAAT CCTTGGTGAT TTAGGCGGCG AAAGAATATT AGAACTGGCA 
TGTGGTGATG AATTGTACGG ACAAGAACAA CAGTTTCGCG CTTGGTCTTC GAAAATATTT 
CAGGTTGCCT GTGAAACGTT TTGCTTAGAT GAAAACGACA TGGTAAAAGA TGCAAAGAAA 
GCTTTGGGTG ACGTACCGCT GACAGAAGAG ACAGTACGTT TCGGAAAATA TCAGGGAGAT 
ACAAAGCTAA AGGCAGCTCT GGAGGCCAGC TTCAGAAAAC AGCTCATTAC TTGTAAAGTT 
AAAGAGAATA AAAACCTAGG GGACTTCTCA GCGGATCGGG CCACAGTATT TGTTGATATG 
CAACCAGAAA CTGAATTCAA ATACGATCCA GGAGATCACG TCGGAGTTTT GGCTTGTAAT 
AGAAAAGAAA TAGTAGATGC AGTACTAGAG AGGATGAAAG ATGTTGACGA CTATGATAAA 
ACTGTTCAAT TACAAGTGAT GAAGGAGACG TTGACACCTA CAGGTGCTAT AAAGACATGG 
GAGCAACATG AAAGATTACC AGCGGTAACG ATACGACAGA TATTTACGAG ATTTCTTGAC 
ATCACGACAC CACCGTCAAC GACTGTACTG AAATACTTAT CTAAAGCTTG CACTGACCAG 
AATGATGCTG CCCAACTTAA AGAATTAGCC ATAGATTCCA ACAAATACGA CGACTGGAGG 
CATCTCCACT ATCCACACTT GGCCGAAGTA CTAGCGCAGT TCCCTTCATG TCGACCTCAA 
GCATCTTTGC TAGCGGCCCT ACTACCATCG TTACAGCCGA GGTTCTACTC CATATCTTCA 
TCACCACTAG CGCATCCTCA TAGAATTCAC ATCACATGCG CTGTTGTTGT TTACAGGACA 
CAGAACGGAC AAGGCCCGAT GCATTATGGA GTATGCTCTA CATACCTACA AAATCTAAAA 
CCGGAGGACG AAGCCCTAGT TTTCATAAGA CGTGCACCAA GCTTCCATAT GCCTAAAGAT 
TTATCAGCAC CGCTTATTCT TGTCGGCCCC GGCTCTGGTA TAGCGCCGTT CAGAGGATTT 
TGGCATCACA GAAAGCATCA GATCAAGAAT TTGATACCGA ACAAACAAAA ACCAGGTCCT 
GTCTGGTTGT TCTTCGGTTG CCGAAACAGA GGAATGGATT TGTATAAAGA AGAAAAAGAA 
AAAGCGTTAG CCGATGGAGT ATTGAGCAAA GTTTTCTTGG CACTTTCTAG GGAAGACTCT 
GTTGAAAAGA AACACATCCA AACGCTACTG GAGGACGAGG GTGCCGAAAT CTCACGGATG 
CTTCTAGACG AAAACGGACA TTTCTACGTT TGTGGTGATT GTAAAATGGC TGAAGAAGTT 
CAACAGAAAT TAAAAGAAAT CATGAAGAAA CATGCCAAAA TGACTGAGCA AGAATATGAA 
GAGTTCATCT TGAACTTGAT GGATGAAAAC AGATACCACG AAGATATCTT CGGGATCACT 
CTGAGAACAG CAGAAGTTCA AAGTGCATCA AGAGAATTCG CTAAAAGGAC AAGGCAGGAA 
TCACAGAAGT CACAAACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012546220.1
CDS	547..4107
Translation	MDQVNGHFVSATCPFSGESVELKVDSNQKQVKPNLRIKVPQPIRLKNHVAHDENFDTLHSRINEVTHFNTKCSEKVCQTSIMDIPGRGDTPRTAEEVFKDAQAFLTQYYASIKRENSEAHKARLDEVKRELKEKGTYQLKTSELVFGAKLAWRNATRCIGRIQWKKLQIFDCREVTTASGMFEALCNHIKYATNKGNIRSAITIFPQRTDGKHDYRIWNPQLINYAGYQEPDGSIIGDPARVEFTEICMKLGWKAPRTPWDILPLVLSADGKDPEFFELPKEIVMEVQFEHPEYDWFKDMGWKWYALPAVSNMRLDCGGLEFTANSFNGWYMGTEIGCRNLCDESRLNIVEAVAKKMGLDTNSFVSLWKDKALVEVNIAVLHSFHRDNVSIVDHHSASEQFQKHLENENKSRGGCPADWIWIVPPMSSSLTTVFHQEMALYYIRPSYDYQEPPWKTHQWTKSDGTKTVHRKFHFKQIARAVKFTSKLFGRALSKRIKATILYATETGKSEHYAKELGTIFGHAFNAQVHCMSEYDMFSIEHETLVLIVASTFGNGEPPANGVDFAEHLFQMLYNETNNNRGDGTNDLGSGTFKTPTPKSLMRSNSMMTPSFEYKRQLTRLESNKSSVAGASSIEQIGPLSNVCFAVFGLGSSAYPKFCHFGKTVDKILGDLGGERILELACGDELYGQEQQFRAWSSKIFQVACETFCLDENDMVKDAKKALGDVPLTEETVRFGKYQGDTKLKAALEASFRKQLITCKVKENKNLGDFSADRATVFVDMQPETEFKYDPGDHVGVLACNRKEIVDAVLERMKDVDDYDKTVQLQVMKETLTPTGAIKTWEQHERLPAVTIRQIFTRFLDITTPPSTTVLKYLSKACTDQNDAAQLKELAIDSNKYDDWRHLHYPHLAEVLAQFPSCRPQASLLAALLPSLQPRFYSISSSPLAHPHRIHITCAVVVYRTQNGQGPMHYGVCSTYLQNLKPEDEALVFIRRAPSFHMPKDLSAPLILVGPGSGIAPFRGFWHHRKHQIKNLIPNKQKPGPVWLFFGCRNRGMDLYKEEKEKALADGVLSKVFLALSREDSVEKKHIQTLLEDEGAEISRMLLDENGHFYVCGDCKMAEEVQQKLKEIMKKHAKMTEQEYEEFILNLMDENRYHEDIFGITLRTAEVQSASREFAKRTRQESQKSQT

Target ORF information:

RefSeq Version	XM_012690766.2
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012690766.2

ORF Insert Sequence:

ATGGATCAAG TTAACGGGCA TTTCGTTTCG GCTACGTGCC CTTTTAGTGG TGAAAGTGTT 
GAGTTGAAAG TTGACAGTAA TCAGAAACAA GTGAAGCCAA ATCTTCGTAT AAAAGTTCCT 
CAACCGATAA GACTAAAGAA CCATGTAGCT CACGACGAGA ACTTCGACAC GCTTCACTCC 
CGGATCAATG AAGTCACTCA TTTTAACACC AAATGCTCAG AAAAAGTTTG TCAGACAAGC 
ATTATGGATA TACCTGGTCG AGGTGATACC CCGCGGACGG CGGAAGAAGT GTTCAAGGAT 
GCTCAGGCAT TCCTCACACA ATACTACGCT TCAATCAAGC GAGAGAACTC AGAAGCCCAC 
AAAGCGAGGT TAGACGAAGT GAAGAGGGAA TTGAAAGAAA AAGGAACGTA TCAACTTAAA 
ACTTCGGAAT TGGTGTTCGG GGCCAAGTTG GCTTGGCGTA ATGCGACCAG GTGCATCGGA 
AGAATACAGT GGAAAAAGTT ACAGATATTC GATTGCAGAG AAGTGACCAC AGCAAGCGGT 
ATGTTCGAAG CATTGTGCAA TCACATCAAA TACGCTACGA ATAAAGGAAA TATTCGTTCA 
GCAATAACGA TCTTCCCACA ACGCACCGAC GGTAAACATG ACTACAGAAT ATGGAATCCG 
CAGCTTATCA ATTATGCAGG ATACCAGGAG CCAGATGGAA GCATTATAGG AGATCCAGCA 
CGAGTTGAAT TTACAGAGAT TTGTATGAAA CTTGGATGGA AAGCGCCGCG CACCCCATGG 
GACATTTTGC CGCTCGTACT CTCTGCTGAC GGAAAAGATC CTGAATTCTT TGAACTACCC 
AAAGAAATAG TTATGGAAGT GCAATTCGAA CATCCTGAAT ATGACTGGTT CAAAGACATG 
GGTTGGAAAT GGTATGCTTT GCCGGCTGTT TCCAACATGA GGCTTGACTG CGGGGGCCTA 
GAGTTCACAG CTAATTCCTT CAACGGCTGG TACATGGGCA CTGAGATCGG ATGCAGGAAC 
CTCTGCGATG AGAGTCGACT GAATATTGTT GAGGCTGTAG CCAAGAAAAT GGGTCTAGAC 
ACAAACTCGT TTGTTTCTCT GTGGAAAGAT AAGGCATTAG TTGAAGTTAA TATAGCGGTA 
CTGCACAGTT TCCATAGAGA TAACGTGTCC ATTGTTGATC ATCACTCCGC GTCCGAACAG 
TTCCAGAAGC ATTTAGAAAA TGAAAATAAA AGCAGGGGCG GATGTCCAGC TGATTGGATA 
TGGATCGTTC CACCGATGTC TTCATCGTTA ACCACAGTGT TCCACCAAGA AATGGCCTTG 
TATTACATCA GACCGTCATA TGATTATCAG GAACCACCAT GGAAGACTCA CCAATGGACA 
AAGTCTGACG GCACTAAGAC CGTCCACAGG AAGTTCCACT TCAAACAGAT CGCCAGAGCA 
GTTAAGTTCA CTTCCAAACT GTTCGGACGG GCGCTCTCCA AGCGCATCAA AGCGACTATT 
CTCTACGCAA CAGAGACGGG CAAATCTGAA CACTACGCCA AAGAATTAGG AACTATTTTC 
GGACACGCTT TCAACGCGCA GGTACATTGC ATGTCGGAAT ATGATATGTT TTCTATAGAA 
CATGAGACTT TAGTTCTCAT AGTGGCTTCC ACGTTCGGCA ATGGAGAACC TCCAGCTAAC 
GGAGTCGATT TTGCAGAGCA TCTATTTCAA ATGCTTTATA ATGAGACAAA CAATAATCGA 
GGAGATGGTA CAAATGACTT AGGCTCTGGT ACGTTCAAGA CACCAACACC AAAATCACTG 
ATGAGATCAA ACAGTATGAT GACACCGAGC TTTGAATATA AGCGGCAATT AACGAGATTA 
GAGTCTAACA AAAGTAGCGT GGCCGGTGCA TCATCTATAG AACAAATAGG ACCTCTTAGC 
AACGTTTGTT TCGCTGTATT TGGTCTGGGG TCGAGTGCGT ACCCAAAATT CTGTCATTTC 
GGTAAAACAG TTGACAAAAT CCTTGGTGAT TTAGGCGGCG AAAGAATATT AGAACTGGCA 
TGTGGTGATG AATTGTACGG ACAAGAACAA CAGTTTCGCG CTTGGTCTTC GAAAATATTT 
CAGGTTGCCT GTGAAACGTT TTGCTTAGAT GAAAACGACA TGGTAAAAGA TGCAAAGAAA 
GCTTTGGGTG ACGTACCGCT GACAGAAGAG ACAGTACGTT TCGGAAAATA TCAGGGAGAT 
ACAAAGCTAA AGGCAGCTCT GGAGGCCAGC TTCAGAAAAC AGCTCATTAC TTGTAAAGTT 
AAAGAGAATA AAAACCTAGG GGACTTCTCA GCGGATCGGG CCACAGTATT TGTTGATATG 
CAACCAGAAA CTGAATTCAA ATACGATCCA GGAGATCACG TCGGAGTTTT GGCTTGTAAT 
AGAAAAGAAA TAGTAGATGC AGTACTAGAG AGGATGAAAG ATGTTGACGA CTATGATAAA 
ACTGTTCAAT TACAAGTGAT GAAGGAGACG TTGACACCTA CAGGTGCTAT AAAGACATGG 
GAGCAACATG AAAGATTACC AGCGGTAACG ATACGACAGA TATTTACGAG ATTTCTTGAC 
ATCACGACAC CACCGTCAAC GACTGTACTG AAATACTTAT CTAAAGCTTG CACTGACCAG 
AATGATGCTG CCCAACTTAA AGAATTAGCC ATAGATTCCA ACAAATACGA CGACTGGAGG 
CATCTCCACT ATCCACACTT GGCCGAAGTA CTAGCGCAGT TCCCTTCATG TCGACCTCAA 
GCATCTTTGC TAGCGGCCCT ACTACCATCG TTACAGCCGA GGTTCTACTC CATATCTTCA 
TCACCACTAG CGCATCCTCA TAGAATTCAC ATCACATGCG CTGTTGTTGT TTACAGGACA 
CAGAACGGAC AAGGCCCGAT GCATTATGGA GTATGCTCTA CATACCTACA AAATCTAAAA 
CCGGAGGACG AAGCCCTAGT TTTCATAAGA CGTGCACCAA GCTTCCATAT GCCTAAAGAT 
TTATCAGCAC CGCTTATTCT TGTCGGCCCC GGCTCTGGTA TAGCGCCGTT CAGAGGATTT 
TGGCATCACA GAAAGCATCA GATCAAGAAT TTGATACCGA ACAAACAAAA ACCAGGTCCT 
GTCTGGTTGT TCTTCGGTTG CCGAAACAGA GGAATGGATT TGTATAAAGA AGAAAAAGAA 
AAAGCGTTAG CCGATGGAGT ATTGAGCAAA GTTTTCTTGG CACTTTCTAG GGAAGACTCT 
GTTGAAAAGA AACACATCCA AACGCTACTG GAGGACGAGG GTGCCGAAAT CTCACGGATG 
CTTCTAGACG AAAACGGACA TTTCTACGTT TGTGGTGATT GTAAAATGGC TGAAGAAGTT 
CAACAGAAAT TAAAAGAAAT CATGAAGAAA CATGCCAAAA TGACTGAGCA AGAATATGAA 
GAGTTCATCT TGAACTTGAT GGATGAAAAC AGATACCACG AAGATATCTT CGGGATCACT 
CTGAGAACAG CAGAAGTTCA AAGTGCATCA AGAGAATTCG CTAAAAGGAC AAGGCAGGAA 
TCACAGAAGT CACAAACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Success in the acquisition of Bombyx mori sperm motility is influenced by the extracellular production of nitric oxide (NO) in the presence of seminal fluid nitric oxide synthase (NOS).
Insect biochemistry and molecular biology8440-47(2017 05)
Nagaoka S,Asagoshi M,Kato K,Takata Y

Nitric oxide synthase during early embryonic development in silkworm Bombyx mori: Gene expression, enzyme activity, and tissue distribution.
Development, growth & differentiation58(9)750-756(2016 Dec)
Kitta R,Kuwamoto M,Yamahama Y,Mase K,Sawada H

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori.
Insect biochemistry and molecular biology38(12)1087-110(2008 Dec)
Tanaka H,Ishibashi J,Fujita K,Nakajima Y,Sagisaka A,Tomimoto K,Suzuki N,Yoshiyama M,Kaneko Y,Iwasaki T,Sunagawa T,Yamaji K,Asaoka A,Mita K,Yamakawa M

cDNA cloning, characterization and gene expression of nitric oxide synthase from the silkworm, Bombyx mori.
Insect molecular biology11(3)257-65(2002 Jun)
Imamura M,Yang J,Yamakawa M

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NOS1 cDNA ORF clone, Bombyx mori(domestic silkworm)

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