Alps cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alps
Entrez Gene ID	692396
Full Name	soluble alkaline phosphatase
Synonyms	Alp-s,S-alp
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043391.2	NP_001036856.2	soluble alkaline phosphatase precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
Suetsugu Y, Futahashi R, Kanamori H, Kadono-Okuda K, Sasanuma S, Narukawa J, Ajimura M, Jouraku A, Namiki N, Shimomura M, Sezutsu H, Osanai-Futahashi M, Suzuki MG, Daimon T, Shinoda T, Taniai K, Asaoka K, Niwa R, Kawaoka S, Katsuma S, Tamura T, Noda H, Kasahara M, Sugano S, Suzuki Y, Fujiwara H, Kataoka H, Arunkumar KP, Tomar A, Nagaraju J, Goldsmith MR, Feng Q, Xia Q, Yamamoto K, Shimada T, Mita K
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)

Tandem duplication of alkaline phosphatase genes and polymorphism in the intergenic sequence in Bombyx mori.
Itoh M, Inoue T, Kanamori Y, Nishida S, Yamaguchi M
Molecular genetics and genomics : MGG270(2)114-20(2003 Nov)

Cloning of soluble alkaline phosphatase cDNA and molecular basis of the polymorphic nature in alkaline phosphatase isozymes of Bombyx mori midgut.
Itoh M, Kanamori Y, Takao M, Eguchi M
Insect biochemistry and molecular biology29(2)121-9(1999 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Alps gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alps cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBg13800	NM_001043391.2 Latest version!	Bombyx mori soluble alkaline phosphatase (Alps), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alps ( NM_001043391.2 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001036856.2 Bombyx mori soluble alkaline phosphatase (Alps), mRNA.

Price & Availability↑

CloneID	OBg13800
Clone ID Related Accession (Same CDS sequence)	NM_001043391.2
Accession Version	NM_001043391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498320000000
Organism	Bombyx mori(domestic silkworm)
Product	soluble alkaline phosphatase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB055428.3. On Mar 2, 2010 this sequence version replaced NM_001043391.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00016912, SAMD00016913 [ECO:0000348] ##Evidence-Data-END##

ATGGTGCGTT TGTTGACACT CCTCTCGCTG CTCGCCGCCG GCTCCTGCGG CCGTCGATAT 
GGATTAGATA AGGACGGCTA CCACCGGGAT GACGTGGGGT CGAGGCGCTC CCTGCAGACG 
CCCTCAACGC CGGCCCCCGA GCTGGAGTCG GAGTACTGGT CGCGCGACGC CCAGAGTGAG 
CTGGGGGAGC GCGCCTGGTA CGACGGCAGC AGCGGGTACG CGCGGAACGT CGTCATGTTC 
CTCGGAGACG GCATGTCCGT GGCCACACTC ACGGCCGCCC GCACGCTGCT CGGCCAGCGC 
CGGGGACAGA CTGGAGAGGA GTCCCGATTG TCATTCGAAC ATTTTCCAAC TGTCGGACTT 
TCTAAGACCT ACTGCCTAGA CGCCCAGGTA GCGGACTCGG CTTGCTCGGC CTCCGCCTAC 
CTGTGCGGTG CAAAGGCTAA CCTGGGCACC ATCGGTGTGT CGGGACACGT GGCGCGGCAC 
CACTGTACTG CGGCCACCGA CGCTGCACAC CAGCTCGCCT CCATCGCCTC CTGGGCTCTC 
GACGCCGACA GGGACGCCGG GATCGTGACC ACCACCCGCG TCACGCACGC CTCGCCGGCC 
GGAGCGTACG CCCACACCGC CGACCGGAAC TGGGAGAGCG ACGGTGACGT CACCGCCGGC 
TGCAGCGGCC ACGCTCAGCT GGACATCGCG CAGCAACTCG TCCACGCGCG TCCCGGGAAA 
GACTTCAAGG TTATTTTGGG AGGCGGAAGA AGAGAGTTCC TACCCAACAC TATGACGGAC 
GAAGAGGGCA GTAGAGGAAG AAGATACGAT GGGCGAAATC TGATCTCAGA GTGGCTCACT 
GAGAAGGACA GTCGCGGCGT CACGCACGAA TACGTGTGGA ACAGACAACA GCTGATGGAG 
GTCTCCGAAG AGTTGCCAGA GTACCTGCTG GGACTATTCG AACCGAGTCA CTTACAATAT 
CATATGGAGG CAAACCATAC CACGGAGCCA ACCCTGGCCG AGCTCACGGA AGTGGCGATC 
AAATCGTTGA GTCGCAACAA GGAAGGATTC TTCTTGTTTG TCGAGGGAGG CAGGATCGAC 
CACGCGCACC ACGAGAACTT GGCTCACCTC GCTCTCGACG AGACCATCGA GCTAAGCGCG 
GCGGTGGAGA AGGCACGGGA GCTGCTCTCG GAGCAGGATT CATTAATCGT AGTGACGGCG 
GACCACGCTC ACGTCATGTC CATCAGCGGG TACACACAGC GCGGCGGGGA TATCCTCGGC 
CCCTCAGACG CGCTGGGCGA CGACGGCATC CCCTACATGA CGCTCTCGTA TGGCAACGGT 
CCCGGGTACC GCGAGCCGAG GGGCGGGCAG CGCGTCGACC CGACACGACA AGATTACCGA 
GGGTCAGAGT ACGTATACCC GGCCGCAGTG CCGCTGGACT CGGAGACTCA CGGCGGAGAC 
GACGTGGCGG TATTCGCCTG GGGGCCTCAC CACGCCCTCT TCTCTGGGCT CTACGAGCAG 
AGTCACATCC CGCATCGCAT GGCGTACGCA GCATGCATCG GTCCCGGCAA GCACGCTTGC 
CAACATTCAC CACATTCAAC TACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036856.2
CDS	1..1587
Translation	MVRLLTLLSLLAAGSCGRRYGLDKDGYHRDDVGSRRSLQTPSTPAPELESEYWSRDAQSELGERAWYDGSSGYARNVVMFLGDGMSVATLTAARTLLGQRRGQTGEESRLSFEHFPTVGLSKTYCLDAQVADSACSASAYLCGAKANLGTIGVSGHVARHHCTAATDAAHQLASIASWALDADRDAGIVTTTRVTHASPAGAYAHTADRNWESDGDVTAGCSGHAQLDIAQQLVHARPGKDFKVILGGGRREFLPNTMTDEEGSRGRRYDGRNLISEWLTEKDSRGVTHEYVWNRQQLMEVSEELPEYLLGLFEPSHLQYHMEANHTTEPTLAELTEVAIKSLSRNKEGFFLFVEGGRIDHAHHENLAHLALDETIELSAAVEKARELLSEQDSLIVVTADHAHVMSISGYTQRGGDILGPSDALGDDGIPYMTLSYGNGPGYREPRGGQRVDPTRQDYRGSEYVYPAAVPLDSETHGGDDVAVFAWGPHHALFSGLYEQSHIPHRMAYAACIGPGKHACQHSPHSTT

Target ORF information:

RefSeq Version	NM_001043391.2
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori soluble alkaline phosphatase (Alps), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043391.2

ORF Insert Sequence:

ATGGTGCGTT TGTTGACACT CCTCTCGCTG CTCGCCGCCG GCTCCTGCGG CCGTCGATAT 
GGATTAGATA AGGACGGCTA CCACCGGGAT GACGTGGGGT CGAGGCGCTC CCTGCAGACG 
CCCTCAACGC CGGCCCCCGA GCTGGAGTCG GAGTACTGGT CGCGCGACGC CCAGAGTGAG 
CTGGGGGAGC GCGCCTGGTA CGACGGCAGC AGCGGGTACG CGCGGAACGT CGTCATGTTC 
CTCGGAGACG GCATGTCCGT GGCCACACTC ACGGCCGCCC GCACGCTGCT CGGCCAGCGC 
CGGGGACAGA CTGGAGAGGA GTCCCGATTG TCATTCGAAC ATTTTCCAAC TGTCGGACTT 
TCTAAGACCT ACTGCCTAGA CGCCCAGGTA GCGGACTCGG CTTGCTCGGC CTCCGCCTAC 
CTGTGCGGTG CAAAGGCTAA CCTGGGCACC ATCGGTGTGT CGGGACACGT GGCGCGGCAC 
CACTGTACTG CGGCCACCGA CGCTGCACAC CAGCTCGCCT CCATCGCCTC CTGGGCTCTC 
GACGCCGACA GGGACGCCGG GATCGTGACC ACCACCCGCG TCACGCACGC CTCGCCGGCC 
GGAGCGTACG CCCACACCGC CGACCGGAAC TGGGAGAGCG ACGGTGACGT CACCGCCGGC 
TGCAGCGGCC ACGCTCAGCT GGACATCGCG CAGCAACTCG TCCACGCGCG TCCCGGGAAA 
GACTTCAAGG TTATTTTGGG AGGCGGAAGA AGAGAGTTCC TACCCAACAC TATGACGGAC 
GAAGAGGGCA GTAGAGGAAG AAGATACGAT GGGCGAAATC TGATCTCAGA GTGGCTCACT 
GAGAAGGACA GTCGCGGCGT CACGCACGAA TACGTGTGGA ACAGACAACA GCTGATGGAG 
GTCTCCGAAG AGTTGCCAGA GTACCTGCTG GGACTATTCG AACCGAGTCA CTTACAATAT 
CATATGGAGG CAAACCATAC CACGGAGCCA ACCCTGGCCG AGCTCACGGA AGTGGCGATC 
AAATCGTTGA GTCGCAACAA GGAAGGATTC TTCTTGTTTG TCGAGGGAGG CAGGATCGAC 
CACGCGCACC ACGAGAACTT GGCTCACCTC GCTCTCGACG AGACCATCGA GCTAAGCGCG 
GCGGTGGAGA AGGCACGGGA GCTGCTCTCG GAGCAGGATT CATTAATCGT AGTGACGGCG 
GACCACGCTC ACGTCATGTC CATCAGCGGG TACACACAGC GCGGCGGGGA TATCCTCGGC 
CCCTCAGACG CGCTGGGCGA CGACGGCATC CCCTACATGA CGCTCTCGTA TGGCAACGGT 
CCCGGGTACC GCGAGCCGAG GGGCGGGCAG CGCGTCGACC CGACACGACA AGATTACCGA 
GGGTCAGAGT ACGTATACCC GGCCGCAGTG CCGCTGGACT CGGAGACTCA CGGCGGAGAC 
GACGTGGCGG TATTCGCCTG GGGGCCTCAC CACGCCCTCT TCTCTGGGCT CTACGAGCAG 
AGTCACATCC CGCATCGCAT GGCGTACGCA GCATGCATCG GTCCCGGCAA GCACGCTTGC 
CAACATTCAC CACATTCAAC TACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)
Suetsugu Y,Futahashi R,Kanamori H,Kadono-Okuda K,Sasanuma S,Narukawa J,Ajimura M,Jouraku A,Namiki N,Shimomura M,Sezutsu H,Osanai-Futahashi M,Suzuki MG,Daimon T,Shinoda T,Taniai K,Asaoka K,Niwa R,Kawaoka S,Katsuma S,Tamura T,Noda H,Kasahara M,Sugano S,Suzuki Y,Fujiwara H,Kataoka H,Arunkumar KP,Tomar A,Nagaraju J,Goldsmith MR,Feng Q,Xia Q,Yamamoto K,Shimada T,Mita K

Tandem duplication of alkaline phosphatase genes and polymorphism in the intergenic sequence in Bombyx mori.
Molecular genetics and genomics : MGG270(2)114-20(2003 Nov)
Itoh M,Inoue T,Kanamori Y,Nishida S,Yamaguchi M

Cloning of soluble alkaline phosphatase cDNA and molecular basis of the polymorphic nature in alkaline phosphatase isozymes of Bombyx mori midgut.
Insect biochemistry and molecular biology29(2)121-9(1999 Feb)
Itoh M,Kanamori Y,Takao M,Eguchi M

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Alps cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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