PLCb1 cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCb1
Entrez Gene ID	100329153
Full Name	phospholipase C beta 1
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001171921.1	NP_001165392.1	phospholipase C beta 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
Suetsugu Y, Futahashi R, Kanamori H, Kadono-Okuda K, Sasanuma S, Narukawa J, Ajimura M, Jouraku A, Namiki N, Shimomura M, Sezutsu H, Osanai-Futahashi M, Suzuki MG, Daimon T, Shinoda T, Taniai K, Asaoka K, Niwa R, Kawaoka S, Katsuma S, Tamura T, Noda H, Kasahara M, Sugano S, Suzuki Y, Fujiwara H, Kataoka H, Arunkumar KP, Tomar A, Nagaraju J, Goldsmith MR, Feng Q, Xia Q, Yamamoto K, Shimada T, Mita K
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)

Gqalpha-linked phospholipase Cbeta1 and phospholipase Cgamma are essential components of the pheromone biosynthesis activating neuropeptide (PBAN) signal transduction cascade.
Hull JJ, Lee JM, Matsumoto S
Insect molecular biology19(4)553-66(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PLCb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBg13774	NM_001171921.1 Latest version!	Bombyx mori phospholipase C beta 1 (PLCb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCb1 ( NM_001171921.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001165392.1 Bombyx mori phospholipase C beta 1 (PLCb1), mRNA.

Price & Availability↑

CloneID	OBg13774
Clone ID Related Accession (Same CDS sequence)	NM_001171921.1
Accession Version	NM_001171921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498320000000
Organism	Bombyx mori(domestic silkworm)
Product	phospholipase C beta 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GU266210.1. ##Evidence-Data-START## Transcript exon combination :: GU266210.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03699703 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGCTA GCACGAGATC AGCCAAAGTT AGCTTAAAGT CCATCGAAGT GCCGAAAGCT 
TTGCAGGATG GAGAAAAATT TATTAAATGG GATGAGGATT CCGGGACAGG CCTTCCAGTT 
ACCTTGAGGG TTGATCCTAA TGGCTTCTAC CTGTACTGGA CTGATCAGAA CATGGAGGTG 
GAGTTGCTAG ACATTGTCAC CATCAGAGAT GTCAGAACTG GAGTTCATGC TAAAGTGCCA 
AAAGATCCGA AAATCCGCAA CGTCGTCCTC ATCGGGAGCC AAGGGTCGCT CGAGGAGAAG 
ACGGTCACGG TTTGTCACGG CACGGACTTC GTCAACGTGA ATTTCATTAA CTTCTGCTGC 
ACTAGGAAGG AAATTGCACA GTTATGGACC GAAGAGTTGT TTGCGTTGGC TTACAACTTG 
AACCAGTTCA ACAATCCAAC GGTGAAGTTT CTAGAGAAAT TGCACACGAA GATAACGTTA 
AAGGCTGACA AATCGGGGAA AATTCTCGTC AAGAACATTG TCCGCCAATT CGCGCAGAAC 
AAGGAAGACA AGAAGCGTGT GGAGAAAGCG CTCAGTGAGT CTGGTCTCCC GACGGGCAAA 
AACGATACCA TCAGCCAGTC CAAGTTCCAG TTCGAAGACT TCTTCGCTTT CTACAAGAGT 
CTCACGCAGA GAACCGAAGT ACAGAAGATA TTTAACGGAC TAACGGACGG AAAACCTTAT 
CTATCAGCGA CTCAATTAGT AGACTTTTTA AACGACGTGC AAAGGGACCC ACGTCTCAAT 
GAGATATTGC ATCCGTACGC TGATCTCCAG CGAGCGAAAG ACCTCATAAA AGCTTACGAA 
CACAATAAGT ATCATCAGCA AAGATCGCAG CTTACCTTCG ATGGATTCCT CAGGTTCCTA 
ATGTCTGAAG ACAATCCTAT AGTGGCACCA AACAAGTTGG ATTTGTGTGA CGATATGGAT 
CAGCCATTAG CTCATTATTT CATCAACAGC TCACACAACA CGTATCTAAC CGGCCATCAG 
ATAACTGGCA AGTCTAGCGT CGAGATATAC AGGCAGAGCT TGCTAGCCGG GTGCAGATGC 
GTGGAGTTGG ACTTCTGGAA TGGTCGAACC GAAGAGCCGG TCATCGTTCA CGGTTACACG 
TTCGTGCCGG AGATAAGCGC CAGGGAAGTG TTGGAGGCGA TAGCGGAAAG CGCTTTCAAG 
ACTTCCGATT TTCCGGTTAT TTTGAGTTTC GAGAACCACT GCAACCCGCG CCAGCAGGCG 
AAGATAGCCA ACTACTGTAG AGAGATTTTC GGGGACATGC TGCTCGATAA GCCTCTGGAC 
TCGCACCAGT TGGAACCAGG AGGTGAACTT CCACCGCCTT CGTTGCTACG GCACAAAATA 
ATAATTAAGA ACAAAAAGAA GCACCATCAC CATCACAAGA AGGAGGACAC ACCGCCGATA 
GAGGAGTGCG AGCAGAGAGC CGAGCTGACA ACGCAGGGGA ACGGCGAGAT GACCCACGCC 
ACATTAGGCA GACAAGGCTC GAAGGATTCG TCCGAGTCGT CGGAGTCGGA GAGCTCGTCT 
GGCGAGGAGG AGGCGGGGGG CGCGGAGGGC GGCGCGGCGG GGGGCGAGGG CGAGCCGCGC 
GAGTGTCACG CCGCCGCCGA GATCTCCGCG CTCGTCAACT ACGTGCAGCC CGTGCACTTT 
AGCTCGTTCG AGAGCGCCGA GAAAAAGAAT CGTTTCTACG AGATGTCATC GTTCGACGAG 
AAACAAGCGA CCACGCTGCT GAAGGAACGT CCCATCGAGT TCGTGAACTA CAACAAGCAC 
CAGCTGTCCC GGGTGTACCC GGCCGGGACC AGGTTCGACT CGTCCAACTT CATGCCACAG 
GTCTTCTGGA ACGCGGGTTG TCAATTAGTC GCTCTCAACT ATCAAACTTT GGACCTCGCC 
ATGCAGCTCA ACCTAGGCAC GTTCGAGTAC AACAAGCGAT GCGGATTCCT ATTGAAACCT 
GAATTTATGA GGAGAAAGGA TCGACGTTTG GATCCATTCG CGGAGAGCAC CGTGGACGGC 
ATCATAGCCG GCAGTCTCAC CGTGACGGTT CTGTCCGGGC AGCTACTGAC CGACAAGCGT 
TGCGGCACGT ACGTGGAGGT GGACATGTTC GGTCTGCCGG CCGACACCGT CAGGAAGAAG 
TTCCGCACCA GGGTCGTGCC CAATAACGGA ATCAATCCGA TTTACGGAGA CGAACCCTTC 
GAATTTAAGA AGGTGGTGCT GCCGGAGCTG GCGATGCTGC GGGTGGCGGC GCACGAGGAG 
GGCGGGCGGC TGCTGGGGCA CCGCGTGGTG CCGGTGCGCG GGCTGCGGCC CGGCCTGCGC 
GCGCTGCCGC TGCGCACGGA GCGGGGCCTG CCGCTGCACG CCGCGCTGCT GCTGCACGTG 
CAGGTCAAGG ACTACGTGCC GGACAAGCTG TCGGAGCTGG CGGAGGCCCT GGCCAACCCC 
ATAAAGTACC AGAGCGAGCT GGACAAGCGC GAGCACCAGC TGGCCGTGCT CACCGACGGC 
ACCGACGAGC CCGAGCAGGA GCCGCGCCGC TCCGTCGCGC AGCCGGTAAG ACACGAGGCG 
GTGCCGGAGA GCAGTCCCAG TAAGTTGGCT TTGGAGACTA AGAAGTTAAT AGAGACCGAG 
TCCATAGTGA CCCTGCCGAT ACCGCCGCCG ATTGATACTA AAGAGCAAGA GAAGAAAGCG 
AACGGCACGG ACGAGAGCCT GGCCGAGACC CTAGAAACGT TGCTGAACTC GAAGCTGGTG 
CGGTTAAAAG AAGCCGAGCT GGCCCGCAAG CTGGACACGC TGCGTCGCAA GTTCGACAAG 
GACAAGAAGC CCTTCTCGGC CAAGTTCTAC GTCAGCAACA AGCTCGTCAA GAAGCTCTCG 
TCCAATAACA TGTGCGGTGA GGCGGGGGGC AGCGGCGCGG CGGAGGAGGG CGCGGCGGAG 
GAGCGCGCGC TGCGGCTGCG CTACCACCAC GCCATCTACG CCGCGCTCGA GAAGCACGTG 
CGCGCCGACC ACTCGCAGCA GATGAAGACG CTCAGCGATC TGTTAGAAGT CGAGAAGAAG 
GAAATATTAA ATCGACTAGC AAACTCGCGG AAGGAAGACG TCAAAGCGCT CGCCAAAAAG 
ACAAATCGAG ATAAAGACGA GATACAGAGG ATAAAGAGAG AGATCCACTC GCAGAGCGTG 
GAGCGCGGCG TGTCGGAATG TTGCCGGCTA GAGCAAGCCT ACACCAAGCG GCGCGAGCAG 
CTCGCCCGCG ACCACGAGAA GCTGCTGCAG ATGCTGCTCA AACATCGAGA TCAGGAGCTC 
GTGAAGCTGG ACCGTCTGTG CGGGGGCACG CCGGAGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165392.1
CDS	1..3342
Translation	MSASTRSAKVSLKSIEVPKALQDGEKFIKWDEDSGTGLPVTLRVDPNGFYLYWTDQNMEVELLDIVTIRDVRTGVHAKVPKDPKIRNVVLIGSQGSLEEKTVTVCHGTDFVNVNFINFCCTRKEIAQLWTEELFALAYNLNQFNNPTVKFLEKLHTKITLKADKSGKILVKNIVRQFAQNKEDKKRVEKALSESGLPTGKNDTISQSKFQFEDFFAFYKSLTQRTEVQKIFNGLTDGKPYLSATQLVDFLNDVQRDPRLNEILHPYADLQRAKDLIKAYEHNKYHQQRSQLTFDGFLRFLMSEDNPIVAPNKLDLCDDMDQPLAHYFINSSHNTYLTGHQITGKSSVEIYRQSLLAGCRCVELDFWNGRTEEPVIVHGYTFVPEISAREVLEAIAESAFKTSDFPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHQLEPGGELPPPSLLRHKIIIKNKKKHHHHHKKEDTPPIEECEQRAELTTQGNGEMTHATLGRQGSKDSSESSESESSSGEEEAGGAEGGAAGGEGEPRECHAAAEISALVNYVQPVHFSSFESAEKKNRFYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQVFWNAGCQLVALNYQTLDLAMQLNLGTFEYNKRCGFLLKPEFMRRKDRRLDPFAESTVDGIIAGSLTVTVLSGQLLTDKRCGTYVEVDMFGLPADTVRKKFRTRVVPNNGINPIYGDEPFEFKKVVLPELAMLRVAAHEEGGRLLGHRVVPVRGLRPGLRALPLRTERGLPLHAALLLHVQVKDYVPDKLSELAEALANPIKYQSELDKREHQLAVLTDGTDEPEQEPRRSVAQPVRHEAVPESSPSKLALETKKLIETESIVTLPIPPPIDTKEQEKKANGTDESLAETLETLLNSKLVRLKEAELARKLDTLRRKFDKDKKPFSAKFYVSNKLVKKLSSNNMCGEAGGSGAAEEGAAEERALRLRYHHAIYAALEKHVRADHSQQMKTLSDLLEVEKKEILNRLANSRKEDVKALAKKTNRDKDEIQRIKREIHSQSVERGVSECCRLEQAYTKRREQLARDHEKLLQMLLKHRDQELVKLDRLCGGTPEG

Target ORF information:

RefSeq Version	NM_001171921.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori phospholipase C beta 1 (PLCb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171921.1

ORF Insert Sequence:

ATGAGTGCTA GCACGAGATC AGCCAAAGTT AGCTTAAAGT CCATCGAAGT GCCGAAAGCT 
TTGCAGGATG GAGAAAAATT TATTAAATGG GATGAGGATT CCGGGACAGG CCTTCCAGTT 
ACCTTGAGGG TTGATCCTAA TGGCTTCTAC CTGTACTGGA CTGATCAGAA CATGGAGGTG 
GAGTTGCTAG ACATTGTCAC CATCAGAGAT GTCAGAACTG GAGTTCATGC TAAAGTGCCA 
AAAGATCCGA AAATCCGCAA CGTCGTCCTC ATCGGGAGCC AAGGGTCGCT CGAGGAGAAG 
ACGGTCACGG TTTGTCACGG CACGGACTTC GTCAACGTGA ATTTCATTAA CTTCTGCTGC 
ACTAGGAAGG AAATTGCACA GTTATGGACC GAAGAGTTGT TTGCGTTGGC TTACAACTTG 
AACCAGTTCA ACAATCCAAC GGTGAAGTTT CTAGAGAAAT TGCACACGAA GATAACGTTA 
AAGGCTGACA AATCGGGGAA AATTCTCGTC AAGAACATTG TCCGCCAATT CGCGCAGAAC 
AAGGAAGACA AGAAGCGTGT GGAGAAAGCG CTCAGTGAGT CTGGTCTCCC GACGGGCAAA 
AACGATACCA TCAGCCAGTC CAAGTTCCAG TTCGAAGACT TCTTCGCTTT CTACAAGAGT 
CTCACGCAGA GAACCGAAGT ACAGAAGATA TTTAACGGAC TAACGGACGG AAAACCTTAT 
CTATCAGCGA CTCAATTAGT AGACTTTTTA AACGACGTGC AAAGGGACCC ACGTCTCAAT 
GAGATATTGC ATCCGTACGC TGATCTCCAG CGAGCGAAAG ACCTCATAAA AGCTTACGAA 
CACAATAAGT ATCATCAGCA AAGATCGCAG CTTACCTTCG ATGGATTCCT CAGGTTCCTA 
ATGTCTGAAG ACAATCCTAT AGTGGCACCA AACAAGTTGG ATTTGTGTGA CGATATGGAT 
CAGCCATTAG CTCATTATTT CATCAACAGC TCACACAACA CGTATCTAAC CGGCCATCAG 
ATAACTGGCA AGTCTAGCGT CGAGATATAC AGGCAGAGCT TGCTAGCCGG GTGCAGATGC 
GTGGAGTTGG ACTTCTGGAA TGGTCGAACC GAAGAGCCGG TCATCGTTCA CGGTTACACG 
TTCGTGCCGG AGATAAGCGC CAGGGAAGTG TTGGAGGCGA TAGCGGAAAG CGCTTTCAAG 
ACTTCCGATT TTCCGGTTAT TTTGAGTTTC GAGAACCACT GCAACCCGCG CCAGCAGGCG 
AAGATAGCCA ACTACTGTAG AGAGATTTTC GGGGACATGC TGCTCGATAA GCCTCTGGAC 
TCGCACCAGT TGGAACCAGG AGGTGAACTT CCACCGCCTT CGTTGCTACG GCACAAAATA 
ATAATTAAGA ACAAAAAGAA GCACCATCAC CATCACAAGA AGGAGGACAC ACCGCCGATA 
GAGGAGTGCG AGCAGAGAGC CGAGCTGACA ACGCAGGGGA ACGGCGAGAT GACCCACGCC 
ACATTAGGCA GACAAGGCTC GAAGGATTCG TCCGAGTCGT CGGAGTCGGA GAGCTCGTCT 
GGCGAGGAGG AGGCGGGGGG CGCGGAGGGC GGCGCGGCGG GGGGCGAGGG CGAGCCGCGC 
GAGTGTCACG CCGCCGCCGA GATCTCCGCG CTCGTCAACT ACGTGCAGCC CGTGCACTTT 
AGCTCGTTCG AGAGCGCCGA GAAAAAGAAT CGTTTCTACG AGATGTCATC GTTCGACGAG 
AAACAAGCGA CCACGCTGCT GAAGGAACGT CCCATCGAGT TCGTGAACTA CAACAAGCAC 
CAGCTGTCCC GGGTGTACCC GGCCGGGACC AGGTTCGACT CGTCCAACTT CATGCCACAG 
GTCTTCTGGA ACGCGGGTTG TCAATTAGTC GCTCTCAACT ATCAAACTTT GGACCTCGCC 
ATGCAGCTCA ACCTAGGCAC GTTCGAGTAC AACAAGCGAT GCGGATTCCT ATTGAAACCT 
GAATTTATGA GGAGAAAGGA TCGACGTTTG GATCCATTCG CGGAGAGCAC CGTGGACGGC 
ATCATAGCCG GCAGTCTCAC CGTGACGGTT CTGTCCGGGC AGCTACTGAC CGACAAGCGT 
TGCGGCACGT ACGTGGAGGT GGACATGTTC GGTCTGCCGG CCGACACCGT CAGGAAGAAG 
TTCCGCACCA GGGTCGTGCC CAATAACGGA ATCAATCCGA TTTACGGAGA CGAACCCTTC 
GAATTTAAGA AGGTGGTGCT GCCGGAGCTG GCGATGCTGC GGGTGGCGGC GCACGAGGAG 
GGCGGGCGGC TGCTGGGGCA CCGCGTGGTG CCGGTGCGCG GGCTGCGGCC CGGCCTGCGC 
GCGCTGCCGC TGCGCACGGA GCGGGGCCTG CCGCTGCACG CCGCGCTGCT GCTGCACGTG 
CAGGTCAAGG ACTACGTGCC GGACAAGCTG TCGGAGCTGG CGGAGGCCCT GGCCAACCCC 
ATAAAGTACC AGAGCGAGCT GGACAAGCGC GAGCACCAGC TGGCCGTGCT CACCGACGGC 
ACCGACGAGC CCGAGCAGGA GCCGCGCCGC TCCGTCGCGC AGCCGGTAAG ACACGAGGCG 
GTGCCGGAGA GCAGTCCCAG TAAGTTGGCT TTGGAGACTA AGAAGTTAAT AGAGACCGAG 
TCCATAGTGA CCCTGCCGAT ACCGCCGCCG ATTGATACTA AAGAGCAAGA GAAGAAAGCG 
AACGGCACGG ACGAGAGCCT GGCCGAGACC CTAGAAACGT TGCTGAACTC GAAGCTGGTG 
CGGTTAAAAG AAGCCGAGCT GGCCCGCAAG CTGGACACGC TGCGTCGCAA GTTCGACAAG 
GACAAGAAGC CCTTCTCGGC CAAGTTCTAC GTCAGCAACA AGCTCGTCAA GAAGCTCTCG 
TCCAATAACA TGTGCGGTGA GGCGGGGGGC AGCGGCGCGG CGGAGGAGGG CGCGGCGGAG 
GAGCGCGCGC TGCGGCTGCG CTACCACCAC GCCATCTACG CCGCGCTCGA GAAGCACGTG 
CGCGCCGACC ACTCGCAGCA GATGAAGACG CTCAGCGATC TGTTAGAAGT CGAGAAGAAG 
GAAATATTAA ATCGACTAGC AAACTCGCGG AAGGAAGACG TCAAAGCGCT CGCCAAAAAG 
ACAAATCGAG ATAAAGACGA GATACAGAGG ATAAAGAGAG AGATCCACTC GCAGAGCGTG 
GAGCGCGGCG TGTCGGAATG TTGCCGGCTA GAGCAAGCCT ACACCAAGCG GCGCGAGCAG 
CTCGCCCGCG ACCACGAGAA GCTGCTGCAG ATGCTGCTCA AACATCGAGA TCAGGAGCTC 
GTGAAGCTGG ACCGTCTGTG CGGGGGCACG CCGGAGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)
Suetsugu Y,Futahashi R,Kanamori H,Kadono-Okuda K,Sasanuma S,Narukawa J,Ajimura M,Jouraku A,Namiki N,Shimomura M,Sezutsu H,Osanai-Futahashi M,Suzuki MG,Daimon T,Shinoda T,Taniai K,Asaoka K,Niwa R,Kawaoka S,Katsuma S,Tamura T,Noda H,Kasahara M,Sugano S,Suzuki Y,Fujiwara H,Kataoka H,Arunkumar KP,Tomar A,Nagaraju J,Goldsmith MR,Feng Q,Xia Q,Yamamoto K,Shimada T,Mita K

Gqalpha-linked phospholipase Cbeta1 and phospholipase Cgamma are essential components of the pheromone biosynthesis activating neuropeptide (PBAN) signal transduction cascade.
Insect molecular biology19(4)553-66(2010 Aug)
Hull JJ,Lee JM,Matsumoto S

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PLCb1 cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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