Hsc70-3 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsc70-3
Entrez Gene ID	409587
Full Name	heat shock 70 kDa protein cognate 3
Synonyms	GB15016
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001160052.1	NP_001153524.1	heat shock protein cognate 3 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Age and natural metabolically-intensive behavior affect oxidative stress and antioxidant mechanisms.
Williams JB, Roberts SP, Elekonich MM
Experimental gerontology43(6)538-49(2008 Jun)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

Annotated expressed sequence tags and cDNA microarrays for studies of brain and behavior in the honey bee.
Whitfield CW, Band MR, Bonaldo MF, Kumar CG, Liu L, Pardinas JR, Robertson HM, Soares MB, Robinson GE
Genome research12(4)555-66(2002 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hsc70-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsc70-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00319	NM_001160052.1 Latest version!	Apis mellifera heat shock 70 kDa protein cognate 3 (Hsc70-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsc70-3 ( NM_001160052.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001153524.1 Apis mellifera heat shock 70 kDa protein cognate 3 (Hsc70-3), mRNA.

Price & Availability↑

CloneID	OHd00319
Clone ID Related Accession (Same CDS sequence)	NM_001160052.1
Accession Version	NM_001160052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1545062400000
Organism	Apis mellifera(honey bee)
Product	heat shock protein cognate 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB736235.1, BI511008.1, QIUM02000013.1, BI513594.1, DB768115.1, DB757570.1 and DB762663.1. On May 10, 2009 this sequence version replaced XM_393090.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: HP517235.1, HP476327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01985570, SAMN02717013 [ECO:0000348] ##Evidence-Data-END##

ATGAAAAAAA TGAAAGGAGT TAAAGCTCTA TTTTTATTAG GCTTAATAAC TTTTGCTTTT 
GCAAAAGAAG AAAAACAGAA AGAAGATATT GGAACTGTTA TTGGAATTGA TTTAGGCACA 
ACATATTCTT GTGTTGGAGT TTATAAAAAT GGAAGAGCAG AAATTATAGC TAATGATCAA 
GGAAATAGAA TTACACCATC ATATGTGGCA TTTACTGCTG ATGGTGAACG ATTAATTGGA 
GATGCTGCAA AGAATCAATT GACTACAAAT CCTGAAAATA CAGTTTTTGA TGCCAAACGA 
TTAATTGGCA GAGAATGGTC AGATCCTACT GTACAACGTG ATATTAAATC CTTTCCATTT 
AAAGTAATTG AAAAAAATAG CAAACCGCAT ATAAGAATGG TAATTAATGG TGAAGAAAAG 
GTATTTGCAC CTGAAGAAAT TTCTGCTATG GTACTTGGTA AAATGAAAGA AACAGCAGAA 
GCATATTTAG GCAAAAAAGT TACTCATGCT GTAGTCACTG TACCAGCTTA TTTTAATGAT 
GCACAAAGAC AAGCTACTAA AGATGCAGGA ACTATTTCTG GACTTGTTGT TATGAGAATT 
ATTAATGAAC CTACAGCTGC TGCAATTGCT TATGGTTTAG ATAAAAAAGA TGGAGAAAAA 
AATGTATTAG TTTTTGATTT AGGTGGTGGT ACTTTTGATG TCAGTTTACT TACAATTGAT 
AATGGAGTAT TTGAAGTTGT GGCAACGAAT GGTGATACTC ATTTAGGTGG TGAAGATTTC 
GATTTACGCG TGATGGATCA TTTTACGAAA CTATATAAAA AGAAAAAAGG CAAAGATATT 
CGTAAAGATA GTAGAACATT ACAAAAATTA CGTCGTGAAG TTGAGAAAGC TAAAAGAGCA 
CTCAGTGTTA GCCACCAAGT GAGGATCGAA ATTGAATCTT TCTTTGAAGG TGAAGACTTT 
TCTGAGACTT TAACACGTGC CAAATTTGAA GAGTTGAATA TGGATCTTTT CCGTAGTACT 
TTGAAACCTG TACAGAAAGT TTTGGAAGAT TCTGATATGA ATAAAAAAGA TGTAGATGAA 
ATTGTACTCG TTGGAGGTTC TACTAGAATT CCTAAAGTTC AACAATTAGT TAAAGAATTC 
TTTGGCGGTA AAGAACCATC ACGAGGTATT AATCCTGACG AGGCTGTTGC ATATGGTGCT 
GCTGTGCAAG CAGGTGTACT TTCTGGGGAA CAAGATACAG ATGCTATTGT ACTTCTGGAT 
GTCAATCCAC TTACTATGGG TATTGAAACA GTTGGAGGTG TAATGACTAA GCTTATTCCA 
AGAAATACTG TAATTCCGAC GAAAAAATCG CAAATCTTTT CTACTGCATC AGACAACCAA 
CATACAGTAA CCATTCAAGT ATATGAAGGT GAACGTCCAA TGACAAAAGA TAATCATCCT 
CTTGGTAAAT TTGATCTTAC TGGCATTCCA CCAGCACCAC GAGGAATACC ACAAATTGAA 
GTTACTTTCG AAATCGATGC TAATGGAATT CTTCAAGTAT CTGCTGAGGA TAAAGGAACT 
GGAAATCGTG AAAAAATTGT TATTACCAAC GATCAAAACC GCCTTACACC CGATGATATA 
GAAAGAATGA TAAAGGATGC AGAGAAATTT GCAGATGATG ATAAAAAATT AAAAGAACGA 
GTAGAAGCAC GTAACGAACT CGAATCGTAT GCATATTCCT TGAAAAATCA ATTGGCCGAT 
AAAGAAAAAC TTGGCTCAAA AGTCAGCGAT TCCGACAAGG CAAAAATGGA AGAAGCTATT 
GATGAAAAGA TTAAATGGCT AGAAGAAAAT GCAGATACTG ATCCAGAAGA ATATAAGAAA 
CAAAAAAAAG AGCTAACAGA TATTGTTCAG CCTATTATTG CCAAATTGTA TCAAGGTGCA 
GGTGGTGGAG TTCCACCAAC TGGTGGAGAT GATGAAGATC TGAAAGATGA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153524.1
CDS	159..2135
Translation	MKKMKGVKALFLLGLITFAFAKEEKQKEDIGTVIGIDLGTTYSCVGVYKNGRAEIIANDQGNRITPSYVAFTADGERLIGDAAKNQLTTNPENTVFDAKRLIGREWSDPTVQRDIKSFPFKVIEKNSKPHIRMVINGEEKVFAPEEISAMVLGKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLVVMRIINEPTAAAIAYGLDKKDGEKNVLVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDLRVMDHFTKLYKKKKGKDIRKDSRTLQKLRREVEKAKRALSVSHQVRIEIESFFEGEDFSETLTRAKFEELNMDLFRSTLKPVQKVLEDSDMNKKDVDEIVLVGGSTRIPKVQQLVKEFFGGKEPSRGINPDEAVAYGAAVQAGVLSGEQDTDAIVLLDVNPLTMGIETVGGVMTKLIPRNTVIPTKKSQIFSTASDNQHTVTIQVYEGERPMTKDNHPLGKFDLTGIPPAPRGIPQIEVTFEIDANGILQVSAEDKGTGNREKIVITNDQNRLTPDDIERMIKDAEKFADDDKKLKERVEARNELESYAYSLKNQLADKEKLGSKVSDSDKAKMEEAIDEKIKWLEENADTDPEEYKKQKKELTDIVQPIIAKLYQGAGGGVPPTGGDDEDLKDEL

Target ORF information:

RefSeq Version	NM_001160052.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera heat shock 70 kDa protein cognate 3 (Hsc70-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160052.1

ORF Insert Sequence:

ATGAAAAAAA TGAAAGGAGT TAAAGCTCTA TTTTTATTAG GCTTAATAAC TTTTGCTTTT 
GCAAAAGAAG AAAAACAGAA AGAAGATATT GGAACTGTTA TTGGAATTGA TTTAGGCACA 
ACATATTCTT GTGTTGGAGT TTATAAAAAT GGAAGAGCAG AAATTATAGC TAATGATCAA 
GGAAATAGAA TTACACCATC ATATGTGGCA TTTACTGCTG ATGGTGAACG ATTAATTGGA 
GATGCTGCAA AGAATCAATT GACTACAAAT CCTGAAAATA CAGTTTTTGA TGCCAAACGA 
TTAATTGGCA GAGAATGGTC AGATCCTACT GTACAACGTG ATATTAAATC CTTTCCATTT 
AAAGTAATTG AAAAAAATAG CAAACCGCAT ATAAGAATGG TAATTAATGG TGAAGAAAAG 
GTATTTGCAC CTGAAGAAAT TTCTGCTATG GTACTTGGTA AAATGAAAGA AACAGCAGAA 
GCATATTTAG GCAAAAAAGT TACTCATGCT GTAGTCACTG TACCAGCTTA TTTTAATGAT 
GCACAAAGAC AAGCTACTAA AGATGCAGGA ACTATTTCTG GACTTGTTGT TATGAGAATT 
ATTAATGAAC CTACAGCTGC TGCAATTGCT TATGGTTTAG ATAAAAAAGA TGGAGAAAAA 
AATGTATTAG TTTTTGATTT AGGTGGTGGT ACTTTTGATG TCAGTTTACT TACAATTGAT 
AATGGAGTAT TTGAAGTTGT GGCAACGAAT GGTGATACTC ATTTAGGTGG TGAAGATTTC 
GATTTACGCG TGATGGATCA TTTTACGAAA CTATATAAAA AGAAAAAAGG CAAAGATATT 
CGTAAAGATA GTAGAACATT ACAAAAATTA CGTCGTGAAG TTGAGAAAGC TAAAAGAGCA 
CTCAGTGTTA GCCACCAAGT GAGGATCGAA ATTGAATCTT TCTTTGAAGG TGAAGACTTT 
TCTGAGACTT TAACACGTGC CAAATTTGAA GAGTTGAATA TGGATCTTTT CCGTAGTACT 
TTGAAACCTG TACAGAAAGT TTTGGAAGAT TCTGATATGA ATAAAAAAGA TGTAGATGAA 
ATTGTACTCG TTGGAGGTTC TACTAGAATT CCTAAAGTTC AACAATTAGT TAAAGAATTC 
TTTGGCGGTA AAGAACCATC ACGAGGTATT AATCCTGACG AGGCTGTTGC ATATGGTGCT 
GCTGTGCAAG CAGGTGTACT TTCTGGGGAA CAAGATACAG ATGCTATTGT ACTTCTGGAT 
GTCAATCCAC TTACTATGGG TATTGAAACA GTTGGAGGTG TAATGACTAA GCTTATTCCA 
AGAAATACTG TAATTCCGAC GAAAAAATCG CAAATCTTTT CTACTGCATC AGACAACCAA 
CATACAGTAA CCATTCAAGT ATATGAAGGT GAACGTCCAA TGACAAAAGA TAATCATCCT 
CTTGGTAAAT TTGATCTTAC TGGCATTCCA CCAGCACCAC GAGGAATACC ACAAATTGAA 
GTTACTTTCG AAATCGATGC TAATGGAATT CTTCAAGTAT CTGCTGAGGA TAAAGGAACT 
GGAAATCGTG AAAAAATTGT TATTACCAAC GATCAAAACC GCCTTACACC CGATGATATA 
GAAAGAATGA TAAAGGATGC AGAGAAATTT GCAGATGATG ATAAAAAATT AAAAGAACGA 
GTAGAAGCAC GTAACGAACT CGAATCGTAT GCATATTCCT TGAAAAATCA ATTGGCCGAT 
AAAGAAAAAC TTGGCTCAAA AGTCAGCGAT TCCGACAAGG CAAAAATGGA AGAAGCTATT 
GATGAAAAGA TTAAATGGCT AGAAGAAAAT GCAGATACTG ATCCAGAAGA ATATAAGAAA 
CAAAAAAAAG AGCTAACAGA TATTGTTCAG CCTATTATTG CCAAATTGTA TCAAGGTGCA 
GGTGGTGGAG TTCCACCAAC TGGTGGAGAT GATGAAGATC TGAAAGATGA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Age and natural metabolically-intensive behavior affect oxidative stress and antioxidant mechanisms.
Experimental gerontology43(6)538-49(2008 Jun)
Williams JB,Roberts SP,Elekonich MM

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

Annotated expressed sequence tags and cDNA microarrays for studies of brain and behavior in the honey bee.
Genome research12(4)555-66(2002 Apr)
Whitfield CW,Band MR,Bonaldo MF,Kumar CG,Liu L,Pardinas JR,Robertson HM,Soares MB,Robinson GE

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Hsc70-3 cDNA ORF clone, Apis mellifera(honey bee)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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