FREM3 cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene
Clones
gRNAs

Summary

Gene Symbol	FREM3
Entrez Gene ID	100476625
Full Name	FRAS1 related extracellular matrix 3
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002916232.2	XP_002916278.2	FRAS1-related extracellular matrix protein 3

Pathways from BioSystems
Gene Ontology

The following FREM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc27487	XM_002916232.2 Latest version!	Ailuropoda melanoleuca FRAS1 related extracellular matrix 3 (FREM3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FREM3 ( XM_002916232.2 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_002916278.2 Ailuropoda melanoleuca FRAS1 related extracellular matrix 3 (FREM3), mRNA.

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CloneID	OAc27487
Clone ID Related Accession (Same CDS sequence)	XM_002916232.2
Accession Version	XM_002916232.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482768000000
Organism	Ailuropoda melanoleuca(giant panda)
Product	FRAS1-related extracellular matrix protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217418.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2015 this sequence version replaced XM_002916232.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGA CTTCTCCGCG CCCCGCTGGG ACGCCCCGGC AGCTTGTCGT GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC GGTGCTACAG GGACAGGTAC CCTCGCCTGG GACTGAACCC 
CACCCTGCGC TTTACCTGCC CACCTGGGGC TCCCTGGCCC GCAGTAGCCC CGACGGCCCC 
AGCGTCCTCA TCGCCAACCC CGGACTCCGG GTTCCCGTGG GCCGCTCGCT TTGGCTGGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCCGGGCACT GGTGCGAGGT GACGGTGCTG 
GACGTCCTGC CGCTGAGCCA CGGTGCGCTC TCTCCGCGCC ACTTCCCCTG CGCCTTCGGG 
CCCCGCCAGG TCCAGTACAC TCACTTAGGC TCCCGAAGCC CGCGACGCGC TCGGGTGCGG 
CTGCAACTGC GCTACGACGC CCCGAATTAC ACGCTGGCTC TGCCCTTCAC GCTGACTGTG 
GACGTGGTCT TCCCCAGGTC GGAGCTGGTG ACGCGCAACA GGCCTTTGTC AGTGGAGAAG 
CTGAGGGGCT GGAGCCAAGC CATCGACAGG AGAGTGCTGG ACTTTGCCTC CCTGGGGAAC 
GGAGCCTCTA CCACCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGATGCCATG GGGGACCCTC TCCCCAGGGG CAAGAGAGTA 
GACTGTGAGA CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACGGCCTC CACCCCGTCG 
CCCAATCGGG ACTATGTGCC CTTGATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGCT 
GGGTCAGAGG CGGTGCTGCC CGGCGAGCAC TTCCAGCTGC TGGTGAGCAT CCTCGACGGA 
GTAGAGAACA CTGCACCTAG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCTCTGAC ACCTGACGCA CTGGCTGCGG AGGACACAGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CGCCAGGGCA CCAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGTCTT CCAGTCTCTT CCTTCACCCA GCGGGAGCTG 
CGGGAACTGA GAATTGCCTA TCAGCCCCCG ACAGAGAATT TGGATTCAGA GCACCTCTTC 
CAGCTGGAGC TGGAGGTGGT GGACGGAGAT GGCGCCACCT CAGACCCCTT TGCCTTCATC 
GTGATAATGA AGCCCGTGAA CACGCTGGCC CCAGTGGCTA GATATAACCA GGGACTGCTG 
CTATTTGAAG GTCAGTTAAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGAGATAAT 
GATAACCTAG AAGAGGTGAT AGTAACTGCA GTAAGGGGCT TGAGACACGG GCAGCTGGTG 
GTGCTTGGGG CACCTGCTGG GTGCAAGCAT TTCACACCAG AGGACCTGGC AGCAGGGCGA 
GTGGCGTACC AGCATGACGG CAGCAACACC TACAGTGACA ATATCATTTT CCGGATGGAG 
GATGGGCACC ACCAGGTGGA ATTCCTCTTC CCTGTCACCA TCATACCTGT TGATGATGAA 
CCACCGAAGG TCAAGGCCAA CACCGGGCTC TCAGTTACAG AAGGCCAGGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC AACTGATATT GACTCTGACG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC GGCCTCTAGA AGAGGAAGAA AGAGGGTGGG ATCTGGCCCC TGGTTCCTCC 
TACTCAACTC AGCACCTGGG GGAGATGCTG CTTCGGCAGG CTGAACCACC TCTGTCCCCG 
GAAGATGAAG ATTGGTATTA TGTGGAAAAG GAAGGACTTT ATGAGAAGGT GGTGACTGAG 
TGGCTTCAGC AAGACATAAT GGAAGGGAAA CTCTTCTATC GCCATCTTGG ACCCCACAGA 
CCTCAACCTG TAATGGCCCA GCTGATTTTC CATGTACAGG ATGACCATGA CCCACCCAAT 
CTATCCAAGC AGCACTTCTT CACCATCAAG GTCCAACCAG TGGATCTGCA GAGTCCAGAA 
CTATTTCCAG GAACTACCCT AGAAATGACC GTGCACCAGT ACCAACTCAC TCACTTCCAG 
AAGAAATTCC TCCAATATGT TGACCAGGAC TCAGATGACC AGAACCTGTG GTACACCTTA 
CTGACACCCC CAACTGACAC AGATGACAAC CACCAAGTCC GGGCTGGAGA AATTGTGCTC 
ACTGATTCAC CAGATATGCC CATCATGCAC TTCACCCAGG CCCAGGTAAA TCTCCATAAA 
GTTGCCTACC AGCCCCCACA GAGGAAGCTG GGTGTTGTAC CACGGGTTGT GCAGTTCACC 
TACCAAGTGG AGGATGCTGC AGGCAACAGC ATACGTGGTA CATTCACATT ATTCCTGAAG 
CCTGTGGACA ACCAGCCTCC AGAAGTCACC AATAGAGGCT TTACTGTCTT AGAGGGAGGA 
AATTTTATCC TCAGCAGTAA TGAACTGGAT GTCATAGACC CTGACACAGA AGTTGACCAG 
ATTGTCTTCA TATTAGTCTG GGGTCCCCAA CATGGACACC TGCGCTACTT AAACAAACAT 
ATGGTTCCAG GGGAACTTTT CATGCAAGCT GATATTATTA ATGGGAGTGT CTCTTACCAG 
CACAGCAGAG ACCAGACCAC CAATGACACC TTCCATCTGG AGGTAAGTGA CAAGGTGCAC 
CACATACCCA TCACCGTCCG GATTTACGTG CATCCCACTT CAGCGGACAA AAGTCCCAAG 
GTCTCTATTA CAGGTAGTCC GTTATTAGAT GTTTCCATAG ATGTACTAGA GAGCAGAGCC 
ACTGAGATCA CCATGGACAT CATACATGGC AAAAAGAAGG GCACGGGTGA CTTGATGCTG 
TCTTTCATTG TAGAGGATAG CCCAAAATTG GGTACTCTTC TAGTGAATGG TTTACCTGCA 
GAACAATTCA CCCAAGAGGA CCTCATCAGT GGGGCAGTAA TCTATGTCCA CACTGGGGGT 
GAAGTTGGCT TCCAGAAACA ACATGATACC TTCAGCCTCA CTCTCTCCAA GGATTCTTGT 
CAATGGGTGG TGGGAGACAG CATAGTTGAG AGAGTGCAGG TGCAAGTGCT TGTGCTGCCT 
GTGGACAATG TGGCACCCAC AGTCTTGGTG GGGGAGTCCT ATATTGTTGA TGAAGGCAGG 
AAGAGTCCCT TGACCTCACA ACATCTCAGT ATTGAAGATG TGGACACACC CCAAGATGAA 
ATCCTATGCA CAGTGACTGG TCAGCCAGCC TCTGGCTACC TGGAAAACAT TGCACCAGCT 
CCTGGTTCAA AAGAATCACG GGCTGGTAGC CCCATCAGTG CTTTCTCCAT CAGAGATGTC 
CAAGCGAGGC ATATCAATTA TGTACAGAGT ATTCACAAGG GCGTAGAGCC ACAAAAAGAT 
CAATTCACCT TTCATTGTTC TGATGGCATC AACTTCTCCC CAAATGTCTT CTTCCCTGAA 
ATCATCTTAC CCACCAATGA TGAGCAGCCT GAACTTTTTG CCAGTGAGTT TGTGGTATTA 
GAGGGGATGA GCCTGGTCAT AGACACCCCA CTGCTCAATG GAGCAGATGC TGACTTGCCA 
CCAAATGAGC TTCACTTTCA GCTCACATCC CTCCCTCAGC ACGGACGAAT CGTACAGCAG 
CTGGCCACCG GCAGCCAGCC CATCCGCAGC TTCACCTTAC AGGAGATCCA GGAGGCCTCC 
ACTATTGTGT ACGAGCATGA TGACTCTGAG ACCACAGAGG ACAGTTTCGA GGTCTGGTTG 
AGTGATGGCA AGCACACCAC TCACAGGAGG GTACCCATTA TGGTGATCCT GGTGGACGAT 
GAAACCCCTC GACTGACCAC CAATGATGGG CTGGAAGTAG AGCCTGGACA CACCGAGGTC 
ATCACAAATT GGGTCCTGAA GGCCACAGAT CTTGACTCTG ATGAAAAGAG CCTCCGTTTC 
GTCCTCCGTT CAGGGCCTCA ACAAGGGCTT CTACAGCGGC TGAGAAAACC CAGAGGGGAG 
GTGAGAAATA ACCTCACGCT AGGGATGAAC TTTACCCAGG ATGAGATTGA CAGAAGCCTC 
ATCTGTTATA CCCACACAGG CCAAGATGGA GTTCTTGACA TTATCAAGTT TGATGTGACT 
GATGGGATTA ACCCTTTGAT AGACCGCTAT TTCTACATCA CTGTTGGCAG CTTAGACAGG 
GTCTTCCCTG AGGCGGTTAG CAAAGGGCTT ACCCTGGCAG AAGGTGGCAG AGTGACCCTT 
ACCACCAGTC TGCTTAGCAC CAGTGACCTG AACAGCCTTA ATGAACAGCT TCACTTCAGA 
ATCACGCGGG CTCCTAGCCT GGGGCACTTG GAGAGCTCTG ACCACCCTGG GAAGCCCATT 
GCCTCTTTCA CTCAGCTTCA GTTGGCTAGC AACAAAATAG TCTATGTCCA CACTTCAAAC 
GATGAGATAA AGACGGACAG CTTTGAGTTT CAAGTGACTG ATGGACTCAA CCCTGTGTCC 
CAGACCTTCA GGATCTTCAT CACAGACGTG GATGATAAGA AACCTGTCTT GACCATCCAT 
AAACTAATAC TGCAGGAAGG GGAAAGCAAA CACATCACTC CGTTTGAGTT GACAGCTGAA 
GACAAGGACA CTCCAGATGA TCTTCTCCTC TTTACTGTCA CCCAGCTCCC CATCCATGGT 
AGGATTTTGT ATAATGGCAG TCATCCTGTG ACCACCTTCA CCAAGCGGGA CCTGAGTGAG 
AACCTGATTA GCTACTGGCA TGATGGCAGT CAGACAACTG AGGACAGTTT CTCCTTGACT 
GTGACAGATG GCACTCATGC TGATTTCTAT GTCTTCCCAG ACACCATCCT AGAGACACAT 
AAACCTCAGG TAATGAGGAT CCACATAAGT TCTCTAGACA ACAGGCTCCC CCAGATTGCC 
ATTAACAGAG GTGCGTCAGC CTTGAAGCGC CTTCACACTG GACACATGAG CTTCCTGATT 
ACCAGCAGGT CTCTGAGGGC AGAGTATCAA GACAGTTTCC AGGGGTTCCT GAAGTATAAA 
GTGACCAGAG GACCAGAGCA TGGTTTCATT ATCAACACTG GCCTTGGAAA CCAGAGCACG 
CGAGTGTTTA TGCAAGCCGA CATTGACGAC AGGAGGATCT GCTACATCTT GAATGAAGGC 
AGCAAAGCAA CAAAGGATAT CTTTTACTTC TCTGTTGAAG ACAACAAAGG AAATAAGCTA 
ACACATCAAC CTTTCCATCT GAACTGGGCT TGGATTTCTT TGGAGAAAGA GTACTACATT 
GTGGATGAAG ATACCACATT CCTAGAAGTG ACCCTCACAC GTAGAGGGTA TCTTGAAGAA 
ACATCCTTTA TCAGCATTGT AACCAAAGAT GAAACTGCGA AGAAAGACAA AGATTTTAAA 
GGAAAGTCCC AGAAACAGGT CCATTTCAAT CCTGGACAGA CTTTGGCCAC ATGGAGAGTG 
AGAATTATAT CTGACAATAA ATACGAGGCT TCAGAGACCT TCCAGATCAT TCTGTTGAAC 
CCTGTCAAGG CTGCACTGGA GTTTCCAGAA ATGGCAACTG TTGAAATTGT GGACCCTGAA 
GATGAATCAA CAGTTTATAT TCCGGAAGCA GAATACAAAA TAGAGGAGCA TGTTGGAGAG 
CTTTTGATTC CAGTAAGACG GTCTGGAGAC GCCGGCCAAG AACTAACAGT CATTTGTTCT 
ACACGTGAAG GTTCTGCCAC AGGCTCCATT CCTTCAACAG CATTATCTTT CTCTGACTAC 
GTCTCACGTC CAGAAGATCG CACCAGCCTC CTCCACTTTG ACAAGGATGA GGCTGAGAAG 
AACTGCCGGG TCTTGATCAT CGATGACTCC CTTTATGAAG AGGAGGAATC CTTTAGTGTT 
TCACTGAGCC TGCCCGTGGG AGGGCGACTG GGAGCCCAGT TCCCCACCAC CAGGGTGGTT 
ATCCTGGCTG ATCACGGTGA TGAGCCCACC CTCCACTTTG GGGATGCCGA GTACCATGTC 
GAGGAAAGTG CTGGCTCCGT GGAGATCTGT GTCTGGAGGA CAGGCCCTGA CCTTTCCCAA 
GCGTCGTCTG TCACTGTGCG CTCCAGGAAG ACAGAGCCAG CAGGAGCAGA AGTTGGAATA 
GATTATGTTG GCATCAACCG ACATCTGGCC TTTGCTCCGG GAGTGCATAA GCAGATATTC 
CAAGTGATGA TCCTTGATGA CCTGGGACAG CCCATCCTGG AGGGTCCAGA GAGGTTTGAA 
TTAGTTCTCC AGAGGCCAGT GGGTGCAGTG CTTGGAGAAC CACATAAAAC CACCATTTTC 
ATCAACGACA CCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002916278.2
CDS	1..6378
Translation	MAATSPRPAGTPRQLVVALAYVLLSCPVLQGQVPSPGTEPHPALYLPTWGSLARSSPDGPSVLIANPGLRVPVGRSLWLDPLRDLVIRVQPGHWCEVTVLDVLPLSHGALSPRHFPCAFGPRQVQYTHLGSRSPRRARVRLQLRYDAPNYTLALPFTLTVDVVFPRSELVTRNRPLSVEKLRGWSQAIDRRVLDFASLGNGASTTSRCRLTLLPPQGGPLPKYGRLVDAMGDPLPRGKRVDCETFVRAGVRYQHTASTPSPNRDYVPLMAELLGPEDRGAGSEAVLPGEHFQLLVSILDGVENTAPRPSFVALMMMEVNQFLLTALTPDALAAEDTESDPDDLVFNILNAPTTTPGHQGYVVSTDDPLGLPVSSFTQRELRELRIAYQPPTENLDSEHLFQLELEVVDGDGATSDPFAFIVIMKPVNTLAPVARYNQGLLLFEGQLKPLSSTHSLQIRDNDNLEEVIVTAVRGLRHGQLVVLGAPAGCKHFTPEDLAAGRVAYQHDGSNTYSDNIIFRMEDGHHQVEFLFPVTIIPVDDEPPKVKANTGLSVTEGQVVQISPFVLSATDIDSDDSTIHFVLEDRPLEEEERGWDLAPGSSYSTQHLGEMLLRQAEPPLSPEDEDWYYVEKEGLYEKVVTEWLQQDIMEGKLFYRHLGPHRPQPVMAQLIFHVQDDHDPPNLSKQHFFTIKVQPVDLQSPELFPGTTLEMTVHQYQLTHFQKKFLQYVDQDSDDQNLWYTLLTPPTDTDDNHQVRAGEIVLTDSPDMPIMHFTQAQVNLHKVAYQPPQRKLGVVPRVVQFTYQVEDAAGNSIRGTFTLFLKPVDNQPPEVTNRGFTVLEGGNFILSSNELDVIDPDTEVDQIVFILVWGPQHGHLRYLNKHMVPGELFMQADIINGSVSYQHSRDQTTNDTFHLEVSDKVHHIPITVRIYVHPTSADKSPKVSITGSPLLDVSIDVLESRATEITMDIIHGKKKGTGDLMLSFIVEDSPKLGTLLVNGLPAEQFTQEDLISGAVIYVHTGGEVGFQKQHDTFSLTLSKDSCQWVVGDSIVERVQVQVLVLPVDNVAPTVLVGESYIVDEGRKSPLTSQHLSIEDVDTPQDEILCTVTGQPASGYLENIAPAPGSKESRAGSPISAFSIRDVQARHINYVQSIHKGVEPQKDQFTFHCSDGINFSPNVFFPEIILPTNDEQPELFASEFVVLEGMSLVIDTPLLNGADADLPPNELHFQLTSLPQHGRIVQQLATGSQPIRSFTLQEIQEASTIVYEHDDSETTEDSFEVWLSDGKHTTHRRVPIMVILVDDETPRLTTNDGLEVEPGHTEVITNWVLKATDLDSDEKSLRFVLRSGPQQGLLQRLRKPRGEVRNNLTLGMNFTQDEIDRSLICYTHTGQDGVLDIIKFDVTDGINPLIDRYFYITVGSLDRVFPEAVSKGLTLAEGGRVTLTTSLLSTSDLNSLNEQLHFRITRAPSLGHLESSDHPGKPIASFTQLQLASNKIVYVHTSNDEIKTDSFEFQVTDGLNPVSQTFRIFITDVDDKKPVLTIHKLILQEGESKHITPFELTAEDKDTPDDLLLFTVTQLPIHGRILYNGSHPVTTFTKRDLSENLISYWHDGSQTTEDSFSLTVTDGTHADFYVFPDTILETHKPQVMRIHISSLDNRLPQIAINRGASALKRLHTGHMSFLITSRSLRAEYQDSFQGFLKYKVTRGPEHGFIINTGLGNQSTRVFMQADIDDRRICYILNEGSKATKDIFYFSVEDNKGNKLTHQPFHLNWAWISLEKEYYIVDEDTTFLEVTLTRRGYLEETSFISIVTKDETAKKDKDFKGKSQKQVHFNPGQTLATWRVRIISDNKYEASETFQIILLNPVKAALEFPEMATVEIVDPEDESTVYIPEAEYKIEEHVGELLIPVRRSGDAGQELTVICSTREGSATGSIPSTALSFSDYVSRPEDRTSLLHFDKDEAEKNCRVLIIDDSLYEEEESFSVSLSLPVGGRLGAQFPTTRVVILADHGDEPTLHFGDAEYHVEESAGSVEICVWRTGPDLSQASSVTVRSRKTEPAGAEVGIDYVGINRHLAFAPGVHKQIFQVMILDDLGQPILEGPERFELVLQRPVGAVLGEPHKTTIFINDTI

Target ORF information:

RefSeq Version	XM_002916232.2
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca FRAS1 related extracellular matrix 3 (FREM3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002916232.2

ORF Insert Sequence:

ATGGCTGCGA CTTCTCCGCG CCCCGCTGGG ACGCCCCGGC AGCTTGTCGT GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC GGTGCTACAG GGACAGGTAC CCTCGCCTGG GACTGAACCC 
CACCCTGCGC TTTACCTGCC CACCTGGGGC TCCCTGGCCC GCAGTAGCCC CGACGGCCCC 
AGCGTCCTCA TCGCCAACCC CGGACTCCGG GTTCCCGTGG GCCGCTCGCT TTGGCTGGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCCGGGCACT GGTGCGAGGT GACGGTGCTG 
GACGTCCTGC CGCTGAGCCA CGGTGCGCTC TCTCCGCGCC ACTTCCCCTG CGCCTTCGGG 
CCCCGCCAGG TCCAGTACAC TCACTTAGGC TCCCGAAGCC CGCGACGCGC TCGGGTGCGG 
CTGCAACTGC GCTACGACGC CCCGAATTAC ACGCTGGCTC TGCCCTTCAC GCTGACTGTG 
GACGTGGTCT TCCCCAGGTC GGAGCTGGTG ACGCGCAACA GGCCTTTGTC AGTGGAGAAG 
CTGAGGGGCT GGAGCCAAGC CATCGACAGG AGAGTGCTGG ACTTTGCCTC CCTGGGGAAC 
GGAGCCTCTA CCACCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGATGCCATG GGGGACCCTC TCCCCAGGGG CAAGAGAGTA 
GACTGTGAGA CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACGGCCTC CACCCCGTCG 
CCCAATCGGG ACTATGTGCC CTTGATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGCT 
GGGTCAGAGG CGGTGCTGCC CGGCGAGCAC TTCCAGCTGC TGGTGAGCAT CCTCGACGGA 
GTAGAGAACA CTGCACCTAG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCTCTGAC ACCTGACGCA CTGGCTGCGG AGGACACAGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CGCCAGGGCA CCAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGTCTT CCAGTCTCTT CCTTCACCCA GCGGGAGCTG 
CGGGAACTGA GAATTGCCTA TCAGCCCCCG ACAGAGAATT TGGATTCAGA GCACCTCTTC 
CAGCTGGAGC TGGAGGTGGT GGACGGAGAT GGCGCCACCT CAGACCCCTT TGCCTTCATC 
GTGATAATGA AGCCCGTGAA CACGCTGGCC CCAGTGGCTA GATATAACCA GGGACTGCTG 
CTATTTGAAG GTCAGTTAAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGAGATAAT 
GATAACCTAG AAGAGGTGAT AGTAACTGCA GTAAGGGGCT TGAGACACGG GCAGCTGGTG 
GTGCTTGGGG CACCTGCTGG GTGCAAGCAT TTCACACCAG AGGACCTGGC AGCAGGGCGA 
GTGGCGTACC AGCATGACGG CAGCAACACC TACAGTGACA ATATCATTTT CCGGATGGAG 
GATGGGCACC ACCAGGTGGA ATTCCTCTTC CCTGTCACCA TCATACCTGT TGATGATGAA 
CCACCGAAGG TCAAGGCCAA CACCGGGCTC TCAGTTACAG AAGGCCAGGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC AACTGATATT GACTCTGACG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC GGCCTCTAGA AGAGGAAGAA AGAGGGTGGG ATCTGGCCCC TGGTTCCTCC 
TACTCAACTC AGCACCTGGG GGAGATGCTG CTTCGGCAGG CTGAACCACC TCTGTCCCCG 
GAAGATGAAG ATTGGTATTA TGTGGAAAAG GAAGGACTTT ATGAGAAGGT GGTGACTGAG 
TGGCTTCAGC AAGACATAAT GGAAGGGAAA CTCTTCTATC GCCATCTTGG ACCCCACAGA 
CCTCAACCTG TAATGGCCCA GCTGATTTTC CATGTACAGG ATGACCATGA CCCACCCAAT 
CTATCCAAGC AGCACTTCTT CACCATCAAG GTCCAACCAG TGGATCTGCA GAGTCCAGAA 
CTATTTCCAG GAACTACCCT AGAAATGACC GTGCACCAGT ACCAACTCAC TCACTTCCAG 
AAGAAATTCC TCCAATATGT TGACCAGGAC TCAGATGACC AGAACCTGTG GTACACCTTA 
CTGACACCCC CAACTGACAC AGATGACAAC CACCAAGTCC GGGCTGGAGA AATTGTGCTC 
ACTGATTCAC CAGATATGCC CATCATGCAC TTCACCCAGG CCCAGGTAAA TCTCCATAAA 
GTTGCCTACC AGCCCCCACA GAGGAAGCTG GGTGTTGTAC CACGGGTTGT GCAGTTCACC 
TACCAAGTGG AGGATGCTGC AGGCAACAGC ATACGTGGTA CATTCACATT ATTCCTGAAG 
CCTGTGGACA ACCAGCCTCC AGAAGTCACC AATAGAGGCT TTACTGTCTT AGAGGGAGGA 
AATTTTATCC TCAGCAGTAA TGAACTGGAT GTCATAGACC CTGACACAGA AGTTGACCAG 
ATTGTCTTCA TATTAGTCTG GGGTCCCCAA CATGGACACC TGCGCTACTT AAACAAACAT 
ATGGTTCCAG GGGAACTTTT CATGCAAGCT GATATTATTA ATGGGAGTGT CTCTTACCAG 
CACAGCAGAG ACCAGACCAC CAATGACACC TTCCATCTGG AGGTAAGTGA CAAGGTGCAC 
CACATACCCA TCACCGTCCG GATTTACGTG CATCCCACTT CAGCGGACAA AAGTCCCAAG 
GTCTCTATTA CAGGTAGTCC GTTATTAGAT GTTTCCATAG ATGTACTAGA GAGCAGAGCC 
ACTGAGATCA CCATGGACAT CATACATGGC AAAAAGAAGG GCACGGGTGA CTTGATGCTG 
TCTTTCATTG TAGAGGATAG CCCAAAATTG GGTACTCTTC TAGTGAATGG TTTACCTGCA 
GAACAATTCA CCCAAGAGGA CCTCATCAGT GGGGCAGTAA TCTATGTCCA CACTGGGGGT 
GAAGTTGGCT TCCAGAAACA ACATGATACC TTCAGCCTCA CTCTCTCCAA GGATTCTTGT 
CAATGGGTGG TGGGAGACAG CATAGTTGAG AGAGTGCAGG TGCAAGTGCT TGTGCTGCCT 
GTGGACAATG TGGCACCCAC AGTCTTGGTG GGGGAGTCCT ATATTGTTGA TGAAGGCAGG 
AAGAGTCCCT TGACCTCACA ACATCTCAGT ATTGAAGATG TGGACACACC CCAAGATGAA 
ATCCTATGCA CAGTGACTGG TCAGCCAGCC TCTGGCTACC TGGAAAACAT TGCACCAGCT 
CCTGGTTCAA AAGAATCACG GGCTGGTAGC CCCATCAGTG CTTTCTCCAT CAGAGATGTC 
CAAGCGAGGC ATATCAATTA TGTACAGAGT ATTCACAAGG GCGTAGAGCC ACAAAAAGAT 
CAATTCACCT TTCATTGTTC TGATGGCATC AACTTCTCCC CAAATGTCTT CTTCCCTGAA 
ATCATCTTAC CCACCAATGA TGAGCAGCCT GAACTTTTTG CCAGTGAGTT TGTGGTATTA 
GAGGGGATGA GCCTGGTCAT AGACACCCCA CTGCTCAATG GAGCAGATGC TGACTTGCCA 
CCAAATGAGC TTCACTTTCA GCTCACATCC CTCCCTCAGC ACGGACGAAT CGTACAGCAG 
CTGGCCACCG GCAGCCAGCC CATCCGCAGC TTCACCTTAC AGGAGATCCA GGAGGCCTCC 
ACTATTGTGT ACGAGCATGA TGACTCTGAG ACCACAGAGG ACAGTTTCGA GGTCTGGTTG 
AGTGATGGCA AGCACACCAC TCACAGGAGG GTACCCATTA TGGTGATCCT GGTGGACGAT 
GAAACCCCTC GACTGACCAC CAATGATGGG CTGGAAGTAG AGCCTGGACA CACCGAGGTC 
ATCACAAATT GGGTCCTGAA GGCCACAGAT CTTGACTCTG ATGAAAAGAG CCTCCGTTTC 
GTCCTCCGTT CAGGGCCTCA ACAAGGGCTT CTACAGCGGC TGAGAAAACC CAGAGGGGAG 
GTGAGAAATA ACCTCACGCT AGGGATGAAC TTTACCCAGG ATGAGATTGA CAGAAGCCTC 
ATCTGTTATA CCCACACAGG CCAAGATGGA GTTCTTGACA TTATCAAGTT TGATGTGACT 
GATGGGATTA ACCCTTTGAT AGACCGCTAT TTCTACATCA CTGTTGGCAG CTTAGACAGG 
GTCTTCCCTG AGGCGGTTAG CAAAGGGCTT ACCCTGGCAG AAGGTGGCAG AGTGACCCTT 
ACCACCAGTC TGCTTAGCAC CAGTGACCTG AACAGCCTTA ATGAACAGCT TCACTTCAGA 
ATCACGCGGG CTCCTAGCCT GGGGCACTTG GAGAGCTCTG ACCACCCTGG GAAGCCCATT 
GCCTCTTTCA CTCAGCTTCA GTTGGCTAGC AACAAAATAG TCTATGTCCA CACTTCAAAC 
GATGAGATAA AGACGGACAG CTTTGAGTTT CAAGTGACTG ATGGACTCAA CCCTGTGTCC 
CAGACCTTCA GGATCTTCAT CACAGACGTG GATGATAAGA AACCTGTCTT GACCATCCAT 
AAACTAATAC TGCAGGAAGG GGAAAGCAAA CACATCACTC CGTTTGAGTT GACAGCTGAA 
GACAAGGACA CTCCAGATGA TCTTCTCCTC TTTACTGTCA CCCAGCTCCC CATCCATGGT 
AGGATTTTGT ATAATGGCAG TCATCCTGTG ACCACCTTCA CCAAGCGGGA CCTGAGTGAG 
AACCTGATTA GCTACTGGCA TGATGGCAGT CAGACAACTG AGGACAGTTT CTCCTTGACT 
GTGACAGATG GCACTCATGC TGATTTCTAT GTCTTCCCAG ACACCATCCT AGAGACACAT 
AAACCTCAGG TAATGAGGAT CCACATAAGT TCTCTAGACA ACAGGCTCCC CCAGATTGCC 
ATTAACAGAG GTGCGTCAGC CTTGAAGCGC CTTCACACTG GACACATGAG CTTCCTGATT 
ACCAGCAGGT CTCTGAGGGC AGAGTATCAA GACAGTTTCC AGGGGTTCCT GAAGTATAAA 
GTGACCAGAG GACCAGAGCA TGGTTTCATT ATCAACACTG GCCTTGGAAA CCAGAGCACG 
CGAGTGTTTA TGCAAGCCGA CATTGACGAC AGGAGGATCT GCTACATCTT GAATGAAGGC 
AGCAAAGCAA CAAAGGATAT CTTTTACTTC TCTGTTGAAG ACAACAAAGG AAATAAGCTA 
ACACATCAAC CTTTCCATCT GAACTGGGCT TGGATTTCTT TGGAGAAAGA GTACTACATT 
GTGGATGAAG ATACCACATT CCTAGAAGTG ACCCTCACAC GTAGAGGGTA TCTTGAAGAA 
ACATCCTTTA TCAGCATTGT AACCAAAGAT GAAACTGCGA AGAAAGACAA AGATTTTAAA 
GGAAAGTCCC AGAAACAGGT CCATTTCAAT CCTGGACAGA CTTTGGCCAC ATGGAGAGTG 
AGAATTATAT CTGACAATAA ATACGAGGCT TCAGAGACCT TCCAGATCAT TCTGTTGAAC 
CCTGTCAAGG CTGCACTGGA GTTTCCAGAA ATGGCAACTG TTGAAATTGT GGACCCTGAA 
GATGAATCAA CAGTTTATAT TCCGGAAGCA GAATACAAAA TAGAGGAGCA TGTTGGAGAG 
CTTTTGATTC CAGTAAGACG GTCTGGAGAC GCCGGCCAAG AACTAACAGT CATTTGTTCT 
ACACGTGAAG GTTCTGCCAC AGGCTCCATT CCTTCAACAG CATTATCTTT CTCTGACTAC 
GTCTCACGTC CAGAAGATCG CACCAGCCTC CTCCACTTTG ACAAGGATGA GGCTGAGAAG 
AACTGCCGGG TCTTGATCAT CGATGACTCC CTTTATGAAG AGGAGGAATC CTTTAGTGTT 
TCACTGAGCC TGCCCGTGGG AGGGCGACTG GGAGCCCAGT TCCCCACCAC CAGGGTGGTT 
ATCCTGGCTG ATCACGGTGA TGAGCCCACC CTCCACTTTG GGGATGCCGA GTACCATGTC 
GAGGAAAGTG CTGGCTCCGT GGAGATCTGT GTCTGGAGGA CAGGCCCTGA CCTTTCCCAA 
GCGTCGTCTG TCACTGTGCG CTCCAGGAAG ACAGAGCCAG CAGGAGCAGA AGTTGGAATA 
GATTATGTTG GCATCAACCG ACATCTGGCC TTTGCTCCGG GAGTGCATAA GCAGATATTC 
CAAGTGATGA TCCTTGATGA CCTGGGACAG CCCATCCTGG AGGGTCCAGA GAGGTTTGAA 
TTAGTTCTCC AGAGGCCAGT GGGTGCAGTG CTTGGAGAAC CACATAAAAC CACCATTTTC 
ATCAACGACA CCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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FREM3 cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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